LOCUS       DACDXE010000001        87975 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.001, whole genome shotgun sequence.
ACCESSION   DACDXE010000001 DACDXE010000000
VERSION     DACDXE010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.001"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(218..1015)
                     /locus_tag="IWM23_000001"
     CDS             complement(218..1015)
                     /locus_tag="IWM23_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAP9882682.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(1012..2196)
                     /locus_tag="IWM23_000002"
     CDS             complement(1012..2196)
                     /locus_tag="IWM23_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9882683.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(2221..3468)
                     /locus_tag="IWM23_000003"
     CDS             complement(2221..3468)
                     /locus_tag="IWM23_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAP9882684.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            3683..5428
                     /gene="dnaX"
                     /locus_tag="IWM23_000004"
     CDS             3683..5428
                     /gene="dnaX"
                     /locus_tag="IWM23_000004"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAP9882685.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            5448..5762
                     /locus_tag="IWM23_000005"
     CDS             5448..5762
                     /locus_tag="IWM23_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAP9882686.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            5787..6383
                     /gene="recR"
                     /locus_tag="IWM23_000006"
     CDS             5787..6383
                     /gene="recR"
                     /locus_tag="IWM23_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAP9882687.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            6447..7091
                     /locus_tag="IWM23_000007"
     CDS             6447..7091
                     /locus_tag="IWM23_000007"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAP9882688.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            7106..7435
                     /locus_tag="IWM23_000008"
     CDS             7106..7435
                     /locus_tag="IWM23_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882689.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            7449..8387
                     /gene="holB"
                     /locus_tag="IWM23_000009"
     CDS             7449..8387
                     /gene="holB"
                     /locus_tag="IWM23_000009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAP9882690.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            8423..9247
                     /locus_tag="IWM23_000010"
     CDS             8423..9247
                     /locus_tag="IWM23_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAP9882691.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            9240..9587
                     /locus_tag="IWM23_000011"
     CDS             9240..9587
                     /locus_tag="IWM23_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAP9882692.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            9656..10528
                     /gene="rsmI"
                     /locus_tag="IWM23_000012"
     CDS             9656..10528
                     /gene="rsmI"
                     /locus_tag="IWM23_000012"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAP9882693.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            10637..11758
                     /gene="dinB"
                     /locus_tag="IWM23_000013"
     CDS             10637..11758
                     /gene="dinB"
                     /locus_tag="IWM23_000013"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAP9882694.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWM23_000014"
     CDS             complement(11812..12414)
                     /gene="nrdG"
                     /locus_tag="IWM23_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAP9882695.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWM23_000015"
     CDS             complement(12729..14918)
                     /gene="nrdD"
                     /locus_tag="IWM23_000015"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAP9882696.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            15244..16011
                     /locus_tag="IWM23_000016"
     CDS             15244..16011
                     /locus_tag="IWM23_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9882697.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            16001..18043
                     /locus_tag="IWM23_000017"
     CDS             16001..18043
                     /locus_tag="IWM23_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9882698.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            18324..18473
                     /locus_tag="IWM23_000018"
     CDS             18324..18473
                     /locus_tag="IWM23_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAP9882699.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            18484..19998
                     /gene="dltA"
                     /locus_tag="IWM23_000019"
     CDS             18484..19998
                     /gene="dltA"
                     /locus_tag="IWM23_000019"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAP9882700.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            19995..21200
                     /gene="dltB"
                     /locus_tag="IWM23_000020"
     CDS             19995..21200
                     /gene="dltB"
                     /locus_tag="IWM23_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAP9882701.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            21245..21478
                     /gene="dltC"
                     /locus_tag="IWM23_000021"
     CDS             21245..21478
                     /gene="dltC"
                     /locus_tag="IWM23_000021"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAP9882702.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            21481..22749
                     /gene="dltD"
                     /locus_tag="IWM23_000022"
     CDS             21481..22749
                     /gene="dltD"
                     /locus_tag="IWM23_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAP9882703.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            22890..23972
                     /locus_tag="IWM23_000023"
     CDS             22890..23972
                     /locus_tag="IWM23_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAP9882704.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            23972..24775
                     /locus_tag="IWM23_000024"
     CDS             23972..24775
                     /locus_tag="IWM23_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9882705.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            24845..25585
                     /locus_tag="IWM23_000025"
     CDS             24845..25585
                     /locus_tag="IWM23_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9882706.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            25596..26600
                     /locus_tag="IWM23_000026"
     CDS             25596..26600
                     /locus_tag="IWM23_000026"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAP9882707.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(26645..28489)
                     /locus_tag="IWM23_000027"
     CDS             complement(26645..28489)
                     /locus_tag="IWM23_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882708.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            28701..29219
                     /locus_tag="IWM23_000028"
     CDS             28701..29219
                     /locus_tag="IWM23_000028"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9882709.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            29347..30030
                     /locus_tag="IWM23_000029"
     CDS             29347..30030
                     /locus_tag="IWM23_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAP9882710.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            30339..30412
                     /locus_tag="IWM23_000030"
     tRNA            30339..30412
                     /locus_tag="IWM23_000030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:30373..30375,aa:Arg,seq:tct)
     gene            30571..30930
                     /locus_tag="IWM23_000031"
     CDS             30571..30930
                     /locus_tag="IWM23_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAP9882711.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            31053..32684
                     /locus_tag="IWM23_000032"
     CDS             31053..32684
                     /locus_tag="IWM23_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAP9882712.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(32746..33387)
                     /locus_tag="IWM23_000033"
     CDS             complement(32746..33387)
                     /locus_tag="IWM23_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9882713.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            33669..33977
                     /gene="rplU"
                     /locus_tag="IWM23_000034"
     CDS             33669..33977
                     /gene="rplU"
                     /locus_tag="IWM23_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAP9882714.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            34004..34348
                     /locus_tag="IWM23_000035"
     CDS             34004..34348
                     /locus_tag="IWM23_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAP9882715.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            34361..34654
                     /gene="rpmA"
                     /locus_tag="IWM23_000036"
     CDS             34361..34654
                     /gene="rpmA"
                     /locus_tag="IWM23_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAP9882716.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            35061..37895
                     /locus_tag="IWM23_000037"
     CDS             35061..37895
                     /locus_tag="IWM23_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9882717.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            38086..38577
                     /locus_tag="IWM23_000038"
     CDS             38086..38577
                     /locus_tag="IWM23_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAP9882718.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            38680..39669
                     /gene="manX"
                     /locus_tag="IWM23_000039"
     CDS             38680..39669
                     /gene="manX"
                     /locus_tag="IWM23_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAP9882719.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            39702..40505
                     /locus_tag="IWM23_000040"
     CDS             39702..40505
                     /locus_tag="IWM23_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAP9882720.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            40526..41440
                     /locus_tag="IWM23_000041"
     CDS             40526..41440
                     /locus_tag="IWM23_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAP9882721.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            41523..41897
                     /locus_tag="IWM23_000042"
     CDS             41523..41897
                     /locus_tag="IWM23_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAP9882722.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            42036..42281
                     /locus_tag="IWM23_000043"
     CDS             42036..42281
                     /locus_tag="IWM23_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9882723.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            42453..43517
                     /locus_tag="IWM23_000044"
     CDS             42453..43517
                     /locus_tag="IWM23_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9882724.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            43763..44188
                     /locus_tag="IWM23_000045"
     CDS             43763..44188
                     /locus_tag="IWM23_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9882725.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            44181..44630
                     /gene="nusB"
                     /locus_tag="IWM23_000046"
     CDS             44181..44630
                     /gene="nusB"
                     /locus_tag="IWM23_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAP9882726.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            44727..45581
                     /locus_tag="IWM23_000047"
     CDS             44727..45581
                     /locus_tag="IWM23_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAP9882727.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            45582..46919
                     /locus_tag="IWM23_000048"
     CDS             45582..46919
                     /locus_tag="IWM23_000048"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAP9882728.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            46924..47148
                     /locus_tag="IWM23_000049"
     CDS             46924..47148
                     /locus_tag="IWM23_000049"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAP9882729.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            47150..48040
                     /locus_tag="IWM23_000050"
     CDS             47150..48040
                     /locus_tag="IWM23_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9882730.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            48049..48867
                     /locus_tag="IWM23_000051"
     CDS             48049..48867
                     /locus_tag="IWM23_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAP9882731.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            48974..49423
                     /locus_tag="IWM23_000052"
     CDS             48974..49423
                     /locus_tag="IWM23_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9882732.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            49441..51123
                     /gene="recN"
                     /locus_tag="IWM23_000053"
     CDS             49441..51123
                     /gene="recN"
                     /locus_tag="IWM23_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAP9882733.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(51178..51294)
                     /locus_tag="IWM23_000054"
     CDS             complement(51178..51294)
                     /locus_tag="IWM23_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAP9882734.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(51367..52323)
                     /locus_tag="IWM23_000055"
     CDS             complement(51367..52323)
                     /locus_tag="IWM23_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9882735.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            52494..52862
                     /locus_tag="IWM23_000056"
     CDS             52494..52862
                     /locus_tag="IWM23_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAP9882736.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            53032..53463
                     /gene="mraZ"
                     /locus_tag="IWM23_000057"
     CDS             53032..53463
                     /gene="mraZ"
                     /locus_tag="IWM23_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAP9882737.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            53484..54443
                     /gene="rsmH"
                     /locus_tag="IWM23_000058"
     CDS             53484..54443
                     /gene="rsmH"
                     /locus_tag="IWM23_000058"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAP9882738.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            54448..54849
                     /gene="ftsL"
                     /locus_tag="IWM23_000059"
     CDS             54448..54849
                     /gene="ftsL"
                     /locus_tag="IWM23_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAP9882739.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            54850..57042
                     /locus_tag="IWM23_000060"
     CDS             54850..57042
                     /locus_tag="IWM23_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9882740.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            57068..58030
                     /locus_tag="IWM23_000061"
     CDS             57068..58030
                     /locus_tag="IWM23_000061"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAP9882741.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            58044..59405
                     /locus_tag="IWM23_000062"
     CDS             58044..59405
                     /locus_tag="IWM23_000062"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAP9882742.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            59431..60519
                     /gene="murG"
                     /locus_tag="IWM23_000063"
     CDS             59431..60519
                     /gene="murG"
                     /locus_tag="IWM23_000063"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9882743.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            60534..61754
                     /locus_tag="IWM23_000064"
     CDS             60534..61754
                     /locus_tag="IWM23_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAP9882744.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            61910..63235
                     /gene="ftsA"
                     /locus_tag="IWM23_000065"
     CDS             61910..63235
                     /gene="ftsA"
                     /locus_tag="IWM23_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAP9882745.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            63258..64499
                     /gene="ftsZ"
                     /locus_tag="IWM23_000066"
     CDS             63258..64499
                     /gene="ftsZ"
                     /locus_tag="IWM23_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAP9882746.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            64516..65193
                     /locus_tag="IWM23_000067"
     CDS             64516..65193
                     /locus_tag="IWM23_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAP9882747.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            65207..65806
                     /locus_tag="IWM23_000068"
     CDS             65207..65806
                     /locus_tag="IWM23_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAP9882748.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            65821..66108
                     /locus_tag="IWM23_000069"
     CDS             65821..66108
                     /locus_tag="IWM23_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAP9882749.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            66157..66939
                     /locus_tag="IWM23_000070"
     CDS             66157..66939
                     /locus_tag="IWM23_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAP9882750.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            66960..67667
                     /locus_tag="IWM23_000071"
     CDS             66960..67667
                     /locus_tag="IWM23_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAP9882751.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            67929..70727
                     /gene="ileS"
                     /locus_tag="IWM23_000072"
     CDS             67929..70727
                     /gene="ileS"
                     /locus_tag="IWM23_000072"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAP9882752.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(70776..72302)
                     /locus_tag="IWM23_000073"
     CDS             complement(70776..72302)
                     /locus_tag="IWM23_000073"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAP9882753.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(72317..72964)
                     /locus_tag="IWM23_000074"
     CDS             complement(72317..72964)
                     /locus_tag="IWM23_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAP9882754.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(73148..73477)
                     /locus_tag="IWM23_000075"
     CDS             complement(73148..73477)
                     /locus_tag="IWM23_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAP9882755.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            73654..74382
                     /gene="sfsA"
                     /locus_tag="IWM23_000076"
     CDS             73654..74382
                     /gene="sfsA"
                     /locus_tag="IWM23_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAP9882756.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            74398..75411
                     /locus_tag="IWM23_000077"
     CDS             74398..75411
                     /locus_tag="IWM23_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9882757.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            75411..76688
                     /locus_tag="IWM23_000078"
     CDS             75411..76688
                     /locus_tag="IWM23_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9882758.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            76751..79453
                     /locus_tag="IWM23_000079"
     CDS             76751..79453
                     /locus_tag="IWM23_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9882759.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            79605..79922
                     /locus_tag="IWM23_000080"
     CDS             79605..79922
                     /locus_tag="IWM23_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9882760.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            79952..80272
                     /locus_tag="IWM23_000081"
     CDS             79952..80272
                     /locus_tag="IWM23_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9882761.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            80380..81840
                     /locus_tag="IWM23_000082"
     CDS             80380..81840
                     /locus_tag="IWM23_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9882762.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            81908..82129
                     /locus_tag="IWM23_000083"
     CDS             81908..82129
                     /locus_tag="IWM23_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882763.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            82160..82342
                     /locus_tag="IWM23_000084"
     CDS             82160..82342
                     /locus_tag="IWM23_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAP9882764.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            82342..82755
                     /locus_tag="IWM23_000085"
     CDS             82342..82755
                     /locus_tag="IWM23_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAP9882765.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            82878..84059
                     /locus_tag="IWM23_000086"
     CDS             82878..84059
                     /locus_tag="IWM23_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9882766.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            84448..84521
                     /locus_tag="IWM23_000087"
     tRNA            84448..84521
                     /locus_tag="IWM23_000087"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:84482..84484,aa:Arg,seq:cct)
     gene            complement(84590..85729)
                     /locus_tag="IWM23_000088"
     CDS             complement(84590..85729)
                     /locus_tag="IWM23_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9882767.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(86028..86663)
                     /locus_tag="IWM23_000089"
     CDS             complement(86028..86663)
                     /locus_tag="IWM23_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882768.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINSASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(86776..87411)
                     /locus_tag="IWM23_000090"
     CDS             complement(86776..87411)
                     /locus_tag="IWM23_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAP9882769.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(87445..87906)
                     /locus_tag="IWM23_000091"
     CDS             complement(87445..87906)
                     /locus_tag="IWM23_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
                     /protein_id="HAP9882770.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
BASE COUNT        28510 a        15067 c        18439 g        25959 t
ORIGIN      
        1 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
       61 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
      121 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
      181 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
      241 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
      301 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
      361 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
      421 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
      481 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
      541 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
      601 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
      661 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
      721 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
      781 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
      841 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
      901 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
      961 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa
     1021 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
     1081 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
     1141 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
     1201 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
     1261 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
     1321 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
     1381 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
     1441 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
     1501 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
     1561 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
     1621 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
     1681 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
     1741 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
     1801 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
     1861 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
     1921 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
     1981 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
     2041 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
     2101 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
     2161 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
     2221 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
     2281 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
     2341 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
     2401 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
     2461 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
     2521 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
     2581 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
     2641 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
     2701 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
     2761 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
     2821 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
     2881 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
     2941 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
     3001 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
     3061 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
     3121 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
     3181 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
     3241 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
     3301 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
     3361 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
     3421 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
     3481 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
     3541 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
     3601 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
     3661 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
     3721 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
     3781 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
     3841 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
     3901 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
     3961 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
     4021 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
     4081 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
     4141 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
     4201 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
     4261 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
     4321 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
     4381 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
     4441 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
     4501 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
     4561 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
     4621 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
     4681 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
     4741 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
     4801 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
     4861 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
     4921 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
     4981 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
     5041 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
     5101 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
     5161 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
     5221 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
     5281 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
     5341 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
     5401 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
     5461 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
     5521 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
     5581 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
     5641 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
     5701 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
     5761 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
     5821 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
     5881 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
     5941 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
     6001 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
     6061 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
     6121 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
     6181 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
     6241 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
     6301 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
     6361 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
     6421 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
     6481 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
     6541 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
     6601 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
     6661 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
     6721 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
     6781 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
     6841 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
     6901 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
     6961 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
     7021 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
     7081 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
     7141 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
     7201 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
     7261 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
     7321 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
     7381 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
     7441 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
     7501 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
     7561 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
     7621 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
     7681 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
     7741 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
     7801 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
     7861 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
     7921 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
     7981 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
     8041 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
     8101 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
     8161 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
     8221 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
     8281 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
     8341 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
     8401 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
     8461 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
     8521 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
     8581 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
     8641 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
     8701 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
     8761 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
     8821 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
     8881 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
     8941 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
     9001 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
     9061 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
     9121 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
     9181 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
     9241 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
     9301 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
     9361 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
     9421 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
     9481 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
     9541 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
     9601 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
     9661 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
     9721 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
     9781 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
     9841 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
     9901 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
     9961 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    10021 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    10081 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    10141 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    10201 gaatttctta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    10261 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    10321 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    10381 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    10441 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    10501 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    10561 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    10621 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    10681 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    10741 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    10801 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    10861 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    10921 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    10981 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    11041 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    11101 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    11161 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    11221 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    11281 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    11341 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    11401 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    11461 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    11521 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    11581 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    11641 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    11701 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    11761 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    11821 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    11881 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    11941 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    12001 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc
    12061 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    12121 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    12181 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    12241 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    12301 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    12361 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    12421 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    12481 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    12541 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    12601 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    12661 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    12721 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    12781 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    12841 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    12901 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    12961 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    13021 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    13081 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    13141 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    13201 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    13261 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    13321 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    13381 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    13441 agacgtttcc cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct
    13501 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    13561 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    13621 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    13681 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    13741 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    13801 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    13861 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    13921 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    13981 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    14041 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    14101 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    14161 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    14221 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    14281 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    14341 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    14401 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    14461 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    14521 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    14581 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    14641 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    14701 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    14761 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    14821 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    14881 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    14941 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    15001 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    15061 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    15121 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    15181 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    15241 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    15301 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    15361 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    15421 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    15481 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    15541 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    15601 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    15661 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    15721 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    15781 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    15841 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    15901 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    15961 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    16021 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    16081 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    16141 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    16201 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    16261 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    16321 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    16381 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    16441 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    16501 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    16561 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    16621 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    16681 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    16741 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    16801 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    16861 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    16921 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    16981 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    17041 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    17101 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    17161 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    17221 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    17281 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    17341 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    17401 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    17461 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    17521 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    17581 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    17641 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    17701 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    17761 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    17821 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    17881 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    17941 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    18001 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    18061 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    18121 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    18181 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    18241 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    18301 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    18361 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    18421 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    18481 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    18541 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    18601 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    18661 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    18721 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    18781 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    18841 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    18901 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    18961 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    19021 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    19081 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    19141 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    19201 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    19261 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    19321 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    19381 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    19441 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    19501 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    19561 aatcccgaaa agacagctgc tgcattcttt tgtcaaaatg acttagcaag ttaccataca
    19621 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    19681 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    19741 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    19801 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    19861 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    19921 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    19981 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    20041 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    20101 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    20161 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    20221 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    20281 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    20341 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    20401 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    20461 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    20521 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    20581 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    20641 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    20701 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    20761 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    20821 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    20881 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    20941 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    21001 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    21061 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    21121 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    21181 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    21241 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    21301 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    21361 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    21421 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    21481 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    21541 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    21601 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    21661 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    21721 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    21781 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    21841 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    21901 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    21961 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    22021 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    22081 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    22141 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    22201 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    22261 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    22321 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    22381 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    22441 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    22501 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    22561 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    22621 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    22681 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    22741 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    22801 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    22861 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    22921 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    22981 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    23041 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    23101 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    23161 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    23221 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    23281 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    23341 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    23401 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    23461 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    23521 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    23581 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    23641 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    23701 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    23761 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    23821 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    23881 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    23941 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    24001 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    24061 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    24121 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    24181 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    24241 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    24301 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    24361 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    24421 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    24481 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    24541 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    24601 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    24661 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    24721 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    24781 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    24841 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    24901 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    24961 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    25021 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    25081 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    25141 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    25201 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    25261 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    25321 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    25381 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    25441 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    25501 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    25561 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    25621 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    25681 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    25741 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    25801 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    25861 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    25921 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    25981 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    26041 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    26101 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    26161 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    26221 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    26281 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    26341 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    26401 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    26461 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    26521 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    26581 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    26641 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    26701 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    26761 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    26821 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    26881 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    26941 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    27001 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    27061 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    27121 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    27181 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    27241 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    27301 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    27361 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    27421 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    27481 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    27541 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    27601 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    27661 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    27721 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    27781 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    27841 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    27901 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    27961 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    28021 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    28081 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    28141 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    28201 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    28261 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    28321 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    28381 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac
    28441 gtaaataaaa agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc
    28501 tttctaaaac ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat
    28561 aaaaaatgag ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca
    28621 tttttacagc tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat
    28681 aaagaaagaa gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa
    28741 cgttgattat atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt
    28801 tgactcctta tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga
    28861 tatatgtacg accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg
    28921 aaaattcagg aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag
    28981 atttttatac aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa
    29041 atattatcgg tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg
    29101 catttgcatg gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag
    29161 catggaatac ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag
    29221 aatgttaaaa acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga
    29281 gaaaacactc atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg
    29341 agtagtatgc gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt
    29401 atgcgtaccc agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat
    29461 gtagcagaag taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga
    29521 cagcttcagc ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc
    29581 atggccacga caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg
    29641 atcgagaaag ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt
    29701 gaaggtcaag cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg
    29761 gatagtgcaa aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa
    29821 ggtggcgttt tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa
    29881 gagattcccc gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg
    29941 atggatcatc ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg
    30001 acaaaggaat cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg
    30061 attgaatcga gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta
    30121 aaggatacag gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc
    30181 aattgataac cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag
    30241 ttgggccaaa ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa
    30301 tttaattatc tataattatt gatgttccgc tgattcttgt catagtagct cagctggata
    30361 gagcatccgc cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa
    30421 ccgttagaaa ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt
    30481 ttcaaaggca aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa
    30541 ataaaactca tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact
    30601 ctagttgcaa ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac
    30661 acgacaagta gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca
    30721 ttgtttaaga accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct
    30781 tatctttctt ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt
    30841 gcattatatg gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct
    30901 ctgttggcat tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag
    30961 gaaactttgt gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt
    31021 ttagaaaatt tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa
    31081 aaaaagaatt ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca
    31141 cagtagctgc tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg
    31201 cggccgcagg tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg
    31261 gtttttatca aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg
    31321 caacaaaggg aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg
    31381 cagcaggact tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg
    31441 ggtttacttc aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg
    31501 ttcactcgga aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc
    31561 cgataaacac agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc
    31621 caaaaaaatg ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag
    31681 ttatgctatt gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca
    31741 gtagtaatcg tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc
    31801 cagctattag cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg
    31861 gccgaatggc aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa
    31921 atctgttgct tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg
    31981 tggccatcaa atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac
    32041 tgctctccat gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag
    32101 gtgttttgat tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta
    32161 ctaataaata ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct
    32221 ttgatttaag tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca
    32281 aaagcgctgc tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag
    32341 aaacggaaaa gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt
    32401 cttcagaaaa actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct
    32461 ccatgcgagg cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag
    32521 aaaaagcaga gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa
    32581 aacaactttc tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg
    32641 cgttgacagc tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac
    32701 agacaagact gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa
    32761 aagtgaagct agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa
    32821 aatagctgct ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc
    32881 atccttttgt tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt
    32941 aaccgtatat tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa
    33001 ttttggctgg accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga
    33061 acgagtaggt gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg
    33121 atgagtcacg cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt
    33181 taagccagtt gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac
    33241 atgcgtatca atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag
    33301 aggtccttgc ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc
    33361 ttgtatcagt tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat
    33421 aaaagaaaca caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt
    33481 attgcttgaa tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt
    33541 ctggtaagct agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta
    33601 ataagcacag catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc
    33661 tttatagcat gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac
    33721 aagcaatcta tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag
    33781 ttatcttagt aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg
    33841 ttgaaggtac tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac
    33901 ctaaaaaaca ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg
    33961 acgcgatcaa cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt
    34021 taagcgaaat gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg
    34081 tccttatgga agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa
    34141 cgggattgcc tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg
    34201 ttatctttat accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat
    34261 tctattagaa aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat
    34321 ccaaattaaa acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct
    34381 acaattattt gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc
    34441 aaaacgtcta ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta
    34501 ccgtcaacgc ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac
    34561 tttatttgca aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca
    34621 agtgtctgtt tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt
    34681 gacaagactt ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac
    34741 gaagtttact ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt
    34801 tgataactat cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat
    34861 atggtaaaca gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt
    34921 cttttaaatt gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat
    34981 agttgacgaa acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct
    35041 atagaaaagg agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag
    35101 aaaaaaccgc tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg
    35161 ctgaagtgtt agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg
    35221 aaggaaaact gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta
    35281 cgcacgtaaa gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag
    35341 aaaaaaatac actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg
    35401 gcagcatgaa aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat
    35461 taaattgttt gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt
    35521 atcattttgc aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt
    35581 gtgctgatta tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag
    35641 gtgcttttac aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta
    35701 tgctgttttt ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact
    35761 ttatggatca tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg
    35821 tacggatcgt aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc
    35881 ggcgtatccc aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag
    35941 tcgatttagt aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt
    36001 tgacagaaga tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat
    36061 tgaagtcaaa tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg
    36121 aaatctcgat cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag
    36181 caagcaaccg cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag
    36241 caccaaatga tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat
    36301 atgatatcat cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa
    36361 tcaatcattt catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg
    36421 gtttttcttt taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag
    36481 tgaccaatca gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact
    36541 ttatttatgc tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag
    36601 aacggcatac gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg
    36661 ttgcgaaaga agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg
    36721 aagactatta cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa
    36781 tcggtgtagt cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc
    36841 aacagttgtt agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga
    36901 aaacagcatt ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca
    36961 tactcttagt agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg
    37021 gaattccagt acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa
    37081 aggtctcagt attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag
    37141 gttacgcaga aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa
    37201 aagctgtttt ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga
    37261 tcgaacgagc ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg
    37321 tggaagtgaa agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg
    37381 ggattactga tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa
    37441 atatcgaagt cattttggat cagttgctga tggaatcaga attggaggaa actgaattca
    37501 tctccctaga tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca
    37561 tctttattaa tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc
    37621 agacgacggg aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag
    37681 caggaatgat cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga
    37741 caaatacaac gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag
    37801 atcagataaa agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata
    37861 atcagttaga taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat
    37921 agtgtatcgt ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa
    37981 aagttggcac gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa
    38041 tgataggggt ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg
    38101 gcacgtatag atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca
    38161 ggtgttgaac gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt
    38221 ctactaaaag aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg
    38281 attcaaatct atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat
    38341 ccccaagatg ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc
    38401 ggtatgagtt ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa
    38461 gatattgatt cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta
    38521 cctgctgaca ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa
    38581 gaatggttgt tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt
    38641 ttgaatttgt tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca
    38701 agtcatggac aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa
    38761 gaaaacgtca aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa
    38821 ttggaagaag cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta
    38881 tggggcggaa caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg
    38941 gcaatcgtca gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca
    39001 atggaatctg ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa
    39061 gtaaaaccgg aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa
    39121 cctaaaggag cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta
    39181 gcacgtgttg actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg
    39241 caaccaaacc gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga
    39301 ctaatcgaac aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg
    39361 atcgaagtag caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat
    39421 cctgaagacg tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg
    39481 tcaatggcgc attcggtcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa
    39541 gacgttgaag cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta
    39601 cctaatgatt cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca
    39661 aaagcgtaat aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa
    39721 ttttagtcgt attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat
    39781 ttcatcaacc actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg
    39841 gtatcgtact tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg
    39901 ctgtagcacc agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg
    39961 gacaaggtgt taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag
    40021 ggttgttctt aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg
    40081 cggctgctga agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt
    40141 tacaaggggt tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg
    40201 tgcaatcatt ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg
    40261 gaatggtcgt agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat
    40321 ggccattctt cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc
    40381 taggtgcttt aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt
    40441 catcaaataa tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact
    40501 attaatcttg aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga
    40561 tcgtttagct gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat
    40621 gcaaaacggt ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa
    40681 agaagaacgt tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat
    40741 tgcttctcca attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc
    40801 tgttgatgat gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt
    40861 tggtgaccca gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt
    40921 agctatggga ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg
    40981 ttgggcattc atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga
    41041 tgacctttct ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt
    41101 cgtattagct gcccttgtac aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac
    41161 tgtaaaatta gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg
    41221 aatcaagagt gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac
    41281 aacattacaa gacaacttag accaattgat tcctggttta gctgcattag gattaacatt
    41341 gttatgtatg tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt
    41401 cgtagtcggt gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg
    41461 gcgttgtccc gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga
    41521 aaatggttca atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg
    41581 gtctgacaga ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc
    41641 gagatagacg caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag
    41701 tcatgctaaa gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat
    41761 acacattttc ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg
    41821 aagatagaat ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta
    41881 tgtttaaacg caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt
    41941 actttgcaga agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata
    42001 tgctaaaatt gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg
    42061 ttggagccgt gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct
    42121 gtgtaacgaa tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg
    42181 aatggggacc aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt
    42241 tggtagcagt tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa
    42301 ctaaaaggag ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa
    42361 gaagttttcc ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga
    42421 aaaaagcgtg caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca
    42481 aaaaaatgca agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt
    42541 acttaacgaa ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt
    42601 ttattactga ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta
    42661 ttaaaaatgt cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc
    42721 actcattagc ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa
    42781 tcatcgatgc ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag
    42841 atgaagaaga aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc
    42901 acatcctgaa aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact
    42961 tttatatgcg ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat
    43021 tgcgttcggc tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga
    43081 tcacattgga ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg
    43141 ctattggtga tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat
    43201 tagcagttat tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta
    43261 gagattatat ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga
    43321 tcggtttaga gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac
    43381 caggaaatgt gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta
    43441 ttgaggatga cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag
    43501 aattgatcat cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag
    43561 actttgttat agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta
    43621 agagataata aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca
    43681 gttttcttac cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt
    43741 aggcaaaggg aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca
    43801 agatttagga gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc
    43861 ttctaaagta gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt
    43921 attaggacgt gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa
    43981 agtagatctt tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat
    44041 gcaggaacgg gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa
    44101 tatccatgta gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt
    44161 tgaagacgaa gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg
    44221 cattgcaggc tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt
    44281 tcaacgcaat cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc
    44341 ctgtttatct tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag
    44401 tgattgaaaa acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg
    44461 tgatcttgcg tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg
    44521 ctttaaatga agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg
    44581 tcaacggtgt tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt
    44641 ttttctatga atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa
    44701 cacaaaaaat atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg
    44761 aaaaaatgca ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc
    44821 ccggattagt cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata
    44881 aagaacgtgt agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga
    44941 tttctgaaga cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg
    45001 gtatattggt ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta
    45061 tccgccctga aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg
    45121 gtaaaccgga caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt
    45181 ctatcgatcc ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac
    45241 cgatggctca gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg
    45301 aaaatctggc tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac
    45361 attttgtcac aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc
    45421 gtgatgctaa cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag
    45481 gctggatcac tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc
    45541 aaaccgtaaa atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa
    45601 ctgtcactac attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac
    45661 gcgtatattt aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt
    45721 tcagcttaaa agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa
    45781 aattacgatt ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat
    45841 atgaagcggg aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg
    45901 ctttatatca agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag
    45961 ggcctaaaaa aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg
    46021 gagcggttat ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg
    46081 ttttgttccc aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc
    46141 agagagtaga agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga
    46201 ttgaggattt atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata
    46261 cgccggtgat ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag
    46321 atgttcgggc tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag
    46381 agttgatgcg cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc
    46441 agcgcaaaca agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa
    46501 gattgtatga aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta
    46561 cacagcaaat ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac
    46621 aagtggctcc gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac
    46681 gcttagaaga gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg
    46741 ttcaatctct tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga
    46801 cgttaaatga tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga
    46861 tccattatca agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa
    46921 aatatggaaa atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag
    46981 ttagaacaag gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa
    47041 ttaagcaaac attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg
    47101 acagaatcaa atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac
    47161 tgacttttca gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga
    47221 gcatacaacg gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa
    47281 aagaatacgt cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt
    47341 tcccgtctat caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga
    47401 tgatttgcct gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt
    47461 attcggcgaa gcattagcta tattggccgg agatggtttg cttacaggag cattccagct
    47521 aatcagtatg gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt
    47581 ttgtgcggga tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa
    47641 aaagctttct ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg
    47701 ttatgccttg ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact
    47761 tcaacgatta gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt
    47821 gattggtaca acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa
    47881 tacttacccg cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt
    47941 atccgccaac aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct
    48001 gatgcgccag atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa
    48061 cgcgtagatg tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt
    48121 tcagtgatgg ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa
    48181 aaaattccag tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga
    48241 gggggattaa aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca
    48301 ttgttagata ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca
    48361 aaaatgagtt atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat
    48421 cctagagtcg tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc
    48481 ttaggaattc cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg
    48541 cctccattac atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa
    48601 ttcgaagcag gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat
    48661 caaaaagttt tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa
    48721 ttaagttatt ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg
    48781 aaagtacccg aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat
    48841 gcacacgaac agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc
    48901 ggattcttct ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag
    48961 gagcgaagac gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga
    49021 gcaagatatc cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt
    49081 tactcaagct acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc
    49141 agatggcggc taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt
    49201 ggaaaaacta ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt
    49261 gaaaacattg ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa
    49321 agaattattt tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga
    49381 ccaggcagtt ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg
    49441 atgctacaag aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt
    49501 cacgaaggaa tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat
    49561 gctttaggtc tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa
    49621 aaatgtgtgc ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg
    49681 gttgaattag ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta
    49741 actggaaaaa atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga
    49801 atcggcagtt atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct
    49861 gaatcacatc ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag
    49921 agttatcagc aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag
    49981 caaaatgaaa aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata
    50041 gctgctgctg atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt
    50101 agcaattatc aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat
    50161 gaacaaaaca gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat
    50221 ctagatgtgg agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa
    50281 gatgcaatcg gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta
    50341 gaagaagtta cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc
    50401 attgagtcaa tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt
    50461 tcagaaggtc aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag
    50521 caacaagccg cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc
    50581 attctgcatg aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta
    50641 aaagaagaaa atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg
    50701 accaatccag gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct
    50761 cggatgttgc ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt
    50821 tttgatgaag tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt
    50881 tctcaaatat cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca
    50941 gtcgccgact atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct
    51001 gtttcagagt tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt
    51061 gagattacaa aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc
    51121 tgagccacct cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta
    51181 catgacactc attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca
    51241 aatgacaaat tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt
    51301 caacctcgtc attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg
    51361 gcttttttat aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa
    51421 tgttaataga actagccata taaaggcatc atccctttca gcaataggta acttaatgtt
    51481 cattccatag atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa
    51541 aattttcata atattattca gattattcga tacgattgct gaaaaagtct gattcatttg
    51601 ttctactagt tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac
    51661 atcacgtaaa tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc
    51721 tttcaatgca tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat
    51781 atccatgatt tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt
    51841 atcgcattga gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat
    51901 gaattgaaag aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa
    51961 gactttcttt aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt
    52021 caaaataata gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc
    52081 aaaaggatat tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc
    52141 tgtacgggaa ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa
    52201 ttgatgtgtc agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt
    52261 ttggtcagtt tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat
    52321 catgattttc ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat
    52381 ggaatgtgtt tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg
    52441 tgtaaagtat atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca
    52501 cattcacacc acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc
    52561 aatttcttgt aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg
    52621 cgattccttt tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta
    52681 tcttaccgtt tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag
    52741 catattatta tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt
    52801 ttctgttgac aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat
    52861 gaaaaaaata gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa
    52921 aaaaatgttt atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt
    52981 gggggattgt ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg
    53041 ggtgaattcc aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt
    53101 gaagaacttg gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat
    53161 ccgatgagcg aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa
    53221 gacgcacgta cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa
    53281 caaggacgaa tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc
    53341 gtagtcatcg gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt
    53401 tctgcagcag cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta
    53461 taagaatagg aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga
    53521 aagaaacggt agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg
    53581 gcggtgccgg acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg
    53641 cgtttgacca agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg
    53701 aacaaggtat ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg
    53761 agcatgtaga taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag
    53821 atgaagcaga acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc
    53881 agtcagctcc tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa
    53941 agattttctt ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac
    54001 gagtacgaaa aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag
    54061 tcatcccagc tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg
    54121 tgcggattgc agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc
    54181 ggttattgaa agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa
    54241 ttgtcaaaag tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg
    54301 tcatacctga cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt
    54361 cagaggaaga gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa
    54421 aaataagaat gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa
    54481 tatgatttac ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat
    54541 caagaaatca ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa
    54601 aaaattgctg tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg
    54661 ctgcgcacaa atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat
    54721 gataaaaagc aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa
    54781 cggattaaac aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa
    54841 gtgaagtaaa tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat
    54901 ccgatgaaca atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc
    54961 tttttatttg ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca
    55021 ttagctgaaa aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga
    55081 acgatttatg atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa
    55141 gcgatactct caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat
    55201 tttgataaaa tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg
    55261 aatattttag aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg
    55321 aaaaatatct cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc
    55381 gctggtcttt attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct
    55441 cattttattg gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt
    55501 ttggagtctg cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa
    55561 gataattttc aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga
    55621 caagatatct acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat
    55681 caagtaaatg aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc
    55741 ggagaaatct tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg
    55801 actggaaaag atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca
    55861 accatgaaag ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa
    55921 accttccaat ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt
    55981 gaaaaaggtg tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc
    56041 atactggaac agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga
    56101 caaagtactc attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt
    56161 gtcgaccgag caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg
    56221 aaagctttca cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa
    56281 gtggtggatc cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct
    56341 ttttcaaaag aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa
    56401 aactacggta gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac
    56461 ggaacagccc aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg
    56521 tattcgattg tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg
    56581 aaacacccta agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa
    56641 cgggcaatgg attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa
    56701 gtatccgtag ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa
    56761 agtggactgc aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc
    56821 aacggtgatc agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg
    56881 atgcctgacg taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt
    56941 attgaagtta gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa
    57001 aaaatcactg aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga
    57061 aaagagcatg gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat
    57121 tgccgcaatg ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg
    57181 tgaggaggga ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt
    57241 gtttctgatc ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac
    57301 tcctactctt ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga
    57361 tgattttata aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat
    57421 tggacagatt ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg
    57481 tgtattgaat ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct
    57541 cttctggctt gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt
    57601 tgctggctta ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca
    57661 gtatgatgta ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta
    57721 caacagaaaa cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact
    57781 tttagcggca atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat
    57841 ctatgttatg gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa
    57901 acgaattttt aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg
    57961 gaagattgat acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat
    58021 cgtttggtag gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc
    58081 ttgtcttagg actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg
    58141 cacttgtcac cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc
    58201 tttctttagg gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat
    58261 tttcattgat ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc
    58321 aagaaatggg tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc
    58381 caattattgg gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac
    58441 tactgaatgc aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag
    58501 caagtggtgt agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct
    58561 tccaactcat gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg
    58621 aagcacatat tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc
    58681 aaaaaaacat gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag
    58741 aattagcgca aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag
    58801 acggtgtgta tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat
    58861 ctgaacttgg cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag
    58921 caaaattgta tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg
    58981 taccacatcg tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa
    59041 aagcaaccaa tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg
    59101 tgttattggc tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa
    59161 aaggaatcaa agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa
    59221 aaaaagcagg catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag
    59281 cattagaaaa cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg
    59341 atcaataccc aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa
    59401 aatagaaaaa gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca
    59461 gggggacata tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat
    59521 gctgaattca tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca
    59581 ggaatgcctt tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac
    59641 atcaagacgg tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat
    59701 tttcaaccag atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct
    59761 gcagccaaaa tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc
    59821 aataaatttt taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc
    59881 ttttttcctg aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat
    59941 actaaaaaat cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg
    60001 atttttggcg gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt
    60061 gaatatattc cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag
    60121 atcctaaata aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat
    60181 atggcagagg tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc
    60241 gcagaattca cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat
    60301 gatcatcaga ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca
    60361 gataatgaac taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa
    60421 gaattgcaaa agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa
    60481 cgtctttatg acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta
    60541 agaaaaaaga accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt
    60601 ggcaaaaggc taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa
    60661 aagaagacat aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag
    60721 aggaacagca agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc
    60781 aaaagaaaaa cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa
    60841 gtcaacggca caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac
    60901 cgctgatttt tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat
    60961 caggcaataa gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag
    61021 aaaatgtttg gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac
    61081 agccaagaat cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg
    61141 taacagaata taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg
    61201 aaaatggaac agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg
    61261 aagagttcaa agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat
    61321 cagaattaca aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc
    61381 tgatccaatt gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga
    61441 accaaatgaa atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca
    61501 tggaagttgg tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc
    61561 aatcggaaga aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag
    61621 aggcagaaac gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt
    61681 caaatgattc aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt
    61741 taaatgaaaa ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa
    61801 tttatggtag aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg
    61861 ctagttgttc gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac
    61921 aggaatgtat gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata
    61981 tatcgacagc caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg
    62041 aggcattatc gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc
    62101 agaagaaaaa gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct
    62161 tgaagtagaa aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga
    62221 tgaagatgtc agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca
    62281 aatcgtgtca atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc
    62341 tcgtggaatg atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac
    62401 gatcatccat aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt
    62461 gattactcct ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac
    62521 gatcgtcatt gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt
    62581 gaagtttacc aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt
    62641 attaaatacg tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc
    62701 agaacggaca tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt
    62761 gaaagtcgat gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa
    62821 caaagcaaaa gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt
    62881 gacaggcgga gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt
    62941 aaatgtaaaa ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt
    63001 aataagtatc gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc
    63061 tgttacgggt gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac
    63121 atatgagagc tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc
    63181 cggagaaaat gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat
    63241 cctgggagga ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa
    63301 tcaaagtcat cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa
    63361 acgttaaagg tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa
    63421 aagctgaaac agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc
    63481 agccagaagt tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag
    63541 acggtgcaga tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg
    63601 caccaatcgt tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac
    63661 gtccattcac ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt
    63721 tgaaagaaaa tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag
    63781 ataaaaagac accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg
    63841 tacaaggtat ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg
    63901 tgaaaacagt gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag
    63961 aagatcgcgt agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta
    64021 ttgatggagc tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg
    64081 aagcacaaga tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc
    64141 taggtacatc tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag
    64201 gtatcgatga atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc
    64261 aatcacctgc tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag
    64321 aagaaaacag ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg
    64381 atgattccaa ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa
    64441 caaaatctca tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa
    64501 ggagaggaag agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat
    64561 tcgtgtgctc ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct
    64621 gttgaatccg ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac
    64681 cgagtagata aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg
    64741 catttaattg gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac
    64801 tttcatgcgt tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa
    64861 ttatcctgtt ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca
    64921 gatgagcttt taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg
    64981 ttgatgacta tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg
    65041 ctgaaagaat tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa
    65101 ttaagtatgg ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc
    65161 cgtgtaggaa cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa
    65221 cagaggagca attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga
    65281 ttatcctgaa acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca
    65341 atcaactcat tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg
    65401 tcctgtaacg catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca
    65461 atcacctaca aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa
    65521 agcaaatctt gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc
    65581 gattgctaaa cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga
    65641 aaaacaagcg agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga
    65701 tatcaaacgt gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag
    65761 ttcaaccgct caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg
    65821 atttatacta ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt
    65881 atcttgatgg tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga
    65941 cgattattag ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg
    66001 attggaccct tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa
    66061 ggtttagcac agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa
    66121 aatctttatt ctcagaatag ataggagggg aaaattttgg atgcaaatgt ataccagcac
    66181 tttcgggcag atgaacgtac atttatcgat tcggtaggcg attggattga acaggttcaa
    66241 ggacaatatg caccctatct gaccgaattt cttgacccaa gacaatccta tattttagaa
    66301 acactgattc gacaaagcag tgatttgcgt ttcatgtttt atggcggata tgaacaggca
    66361 gaaagaaaaa gatgtttgat tttccccgat tattacgaac ctgtagagga agattttgaa
    66421 acagcattgt atgaaatcca ttatcctagc aaatttgcca gcctaagtca tgggaaaatc
    66481 cttggaactt tagtgggaac ggggatcaaa cgctcttact ttggcgatat catctcggac
    66541 ggtgatcgct ggcaagtatt tattgcaaat gaggtggatc attttgttgc tgcacaagta
    66601 acaaagattg gaaaggttgc tgtacgcttg gaaccaatca aatacacaga aatgattatg
    66661 ccgaaagata gctggacaca agaaagagga acagttagtt ccatgcgact agacagtgtg
    66721 atctctgaga tttataatat ttccagacag cggtccaagc agctgatcga agctggtaaa
    66781 gtaaaagtga actggacaga aaatacaaga cctgattttg tacttgaact attagatatt
    66841 atttcagtca gaggttatgg cagagtccag atccaagaac ttgaggggaa gacgaaaaaa
    66901 gaaaagtaca gggtactgtt cggtgtatta cgtaaataaa ataaaaaggt ggtttactca
    66961 tggctttaac tccattagat atccaaaata agacattccc gacaaaaatg cggggatata
    67021 atcaagacga agtagatgat ttcttagatt tagttgttcg tgactatgag gaattgactc
    67081 aaagaaatcg agaattagaa aaagcagtca aacattctga agaaaaactt gagtacttca
    67141 atgaattaaa agatgctttg aaccaatcaa tcatcgtagc tcaagatacg gctgataaag
    67201 taaaaacaag tgcaagcaag gaatccgaag tgatcgttac ttctgcacaa aacaaagcag
    67261 atgagttagt tgcgaacgct gaaaaacgtg cacatcaatt aacaactgat gcagaagaaa
    67321 aagcaagaaa aatcttgaca gatgcaacag aaaaagcaag acagcttgcg acagaaactg
    67381 aagatcttaa gaaaaaaaca cgagtattcc atcaacgtat cagcttgatg cttgagtctc
    67441 aattagaaca agtgaaaagt ccggaatggg atgaaatttt acaaccattc tccagctacg
    67501 tgactgacag ccatgaagtg atcaaagaag ttttagcaaa acagcttgac aatgaaaatg
    67561 atactgatgt aaactcagac acaggagtca tagaagcacc tatgacggca tttgatacac
    67621 aggctattga tatctcgatc gtgcctggtg aaaatgaaaa cgagtagaac agaaaagcac
    67681 tttgttattc ttaaagcgag ccgatgagag tggaagttcg gtaagacact gcatggtgaa
    67741 agatcctcta cttgagacct gcttttccga aattagagta agaagcagcg gctgacctcg
    67801 ttattggtca cagagagttt gcactagata gatgcaacta aatttgggtg gtaccacggc
    67861 atgttcgtcc cttgcggctg aacatgcttt tttgtgtccg gaaaacaaat aaaggagtgt
    67921 atggttgaat gaaaatgaaa gaaaccttgc aattaggaaa aacaaaattc cctatgcgtg
    67981 gtaatttgcc aaaccgagaa ggcgagtggc aaaaagaatg ggaagaaaat cgaatctacc
    68041 aaaaaagaca agagttgaac gaaggaaaac ctagctttgt tcttcatgat ggacctccat
    68101 atgccaatgg taatatccat ttaggacatt cattgaataa aatcagtaaa gacattatca
    68161 ttcgttcaaa atcaatgtct ggctatcgtg caccatatgt tccaggatgg gatacacatg
    68221 gattgccgat tgaacaagta ttggctaata aaggcgtgaa acgtaaagaa atgtccatgg
    68281 cagaatactt ggaaaaatgt cgtgaatatg cactttccca agtagataag caacgagcag
    68341 atttcaaacg cctaggagtt gcaggagatt gggaacatcc ttatgttaca ttagatcctt
    68401 cttatgaagc tgcacaagta cgcgtatttg gtaaaatggc tgaaaaaggc tatatttaca
    68461 aaggattgaa accaatttac tggtctccgt caagtgagtc ttctttagcg gaagctgaaa
    68521 ttgaatataa agatgtaaaa tctccatcta tttttgtagc tttcaatgtc aaagacggca
    68581 aagatattct tgatgaagat acagcttttg tcatttggac gacaacacca tggacattgc
    68641 cagcaaacca aggtattgct gtaaatccaa aatttacgta tgtattggtg gaagccgatg
    68701 gccgtaagtt tgtcgtagcg aaagatttgc tagaaacagt ccaatcagaa atcggttggg
    68761 aaaatgtaac agttctaaaa gaatttgctg gacaagaaat ggaatatatg actgcaagtc
    68821 atccattcta tgaacgtgaa tcacttgtga ttttaggcga tcatgttact ttagacgctg
    68881 gtactggatt agtccatact gctccaggac atggggaaga tgactatatc gctggtagca
    68941 aatataaact atctgtagtg tcacctgtag actcaaaagg attgtttaca gaagaagcac
    69001 ctggatttga aggtatcttt tatgacaaag caaatcctat gatcactgat ctattaaaag
    69061 aaaaaggagc tttgctgaag ctagatttct ttactcatag ttatcctcac gattggcgga
    69121 cgaaaaagcc tgtgatctat cgggcaactc cacaatggtt tgcatctatc gataaattcc
    69181 gtcagaatat tttggatgaa gtagaaaaag tagagtgggt tatcccatgg ggtaaaacac
    69241 gtttgtataa tatgatccgt gaccgcggcg attgggtaat ttcccgtcag cgtgcatggg
    69301 gagtaccact tccgattttc tatgctgaaa atggagaagc aatcatcaca ccagaaacaa
    69361 tcgaacatgt ggcaaaatta tttgctgaac atggatcaat tatctggttt gaaaaagaag
    69421 cgaaagaact tcttcctgaa ggatttaccc acccaggttc tcctaatggc gaatttacaa
    69481 aagaaaaaga tatcatggat gtctggtttg attctggttc ttctcatgaa gctgttttac
    69541 gtcagcgacc agaattatca ttcccagcag atatgtattt agaaggatca gaccaatatc
    69601 gtggctggtt caactcaagt atcacgacta gtgtggctat caatggtgta gcaccataca
    69661 aatcagttat ttctcaagga tttgctttag atggtgaagg ccgtaagatg agtaaatctt
    69721 taggaaacat cattgcacca gaaaaagtga tcaaacaatt tggtgcagat atccttcgct
    69781 tatgggtatc tagtgtagat tatgaagcag atgttcgtgt ttctatggat atcttaagtc
    69841 aagtggcaga agtatatcgt aaaatcagaa atacgatgcg tttcttatta gcgaatacag
    69901 aagatttcga tcctaaaaaa gatactgttt cgtacaatga tttacgttct gtagacaaat
    69961 atatgaccgt tcgattgaac caagtaatca aagaaatccg agaagaaggg tacgacaaat
    70021 ataacttcat gcatatttac cgtacagtta tgaacttctt gacggttgat ctatcgtcat
    70081 tctacctgga ttttgctaaa gatgtagttt atattgaagc agaagatgat tatcaacgtc
    70141 gttgcatgca aactgttttc tatcaaacag ctgtagcttt gacaaaatta ttgactccta
    70201 tcattccgca tacagcagaa gaaatctggt cattcctgaa agaagaagag gagtacgttc
    70261 agttaagcga gtttcctggt tatgaagagt ttgcaaacca agaagaattg atggatacct
    70321 ggactgcttt catggacttc cgtgataatg tcttaaaagc attggaagaa gcaagaaaca
    70381 gcaaattgat tggtaaatct ttggaagcaa aactaactgt ttatccaaaa caacaagttc
    70441 gtgaaatgtt aaaagctttg gatgcagata ttgcacaatt attgatcgtt tcaccagact
    70501 actttgaagt tatggcagca gatgaacaag tgccagataa cgctatggta tttgatgatg
    70561 ttgctattac agtggaaaaa gcagacgggg aaacttgcga tcgctgtcgc caagttcgta
    70621 aagatgtagg agtagatgaa aaacttcctc atctatgcgg tcgttgtgca aaaatcgttg
    70681 aagacttcta tcctgaagct gtagctgaag gattcgaaga aaagtaaaag ggaaaagggg
    70741 gtgtgacatg ttctttgtca cacccccttt tgttatcaat cgtttaattt tccacgttca
    70801 cgataccagt tatctggttg ccagatccat tcgaatccgt ctttttcaag tagttcgaaa
    70861 gctgctttag gtcccattga tccagctttg tagttgggaa aatccggttg tgtttggtcc
    70921 caagcttttc gaatgacatc cacgatatgc caagattgag cgacttcttc ccaatgagag
    70981 aagttcgttc catcaccatt caacgcatct aatagtaatt tttcgtaagc ttctgggcta
    71041 ttttcaacca tttctgcact atttctaaat tccaatttga ttggttccgt ttcaaatcct
    71101 tgccctactt ctttcccatt caaggataaa gaaaatcctt cagttggctg aatatagatc
    71161 gttagaacat taggtggaag tgtcgtatcg tcacatggtt catcgacaga tgttttgaat
    71221 acattaacag gaacttgttt gaatacgatg ttgatccgag tccctttttc agtcaaacgt
    71281 ttgcctgtac gtacatagaa aggaacacct gaccaacgga agttatcgat agtgaatttg
    71341 cctgcgacga atgtttcagt ctctgattga gcatccactt tgtcttcttc gcgatagccc
    71401 ttgaattgta caccatccaa ttcaccttga gcatattgcc cgcgaacgaa gttttctaac
    71461 acttcttctt cgttataaat gcggatagct ttcaatgctt tgatcttttc tgtacggatt
    71521 tctttttcgg aaaatgctac tggcggttcc attgcaagta atgctacaac ctgtaaaata
    71581 tgattttgca ccatgtcttt aagtgcgcca ctatgatcgt agtagccccc gcgatcttct
    71641 acaccgaggt tttcgccaaa agtaatttgg atattgtcga tatagcgatt gttccattga
    71701 gcctcaaaaa tattattggc aaaacggata gctgagatgt tttggatcat ttcttttcct
    71761 aaataatgat caatcctgaa aatatcttgt tctgggaata cttctctgat ttgctggttc
    71821 aattcaaaag cagactggta atcagaaccg aacggttttt ctatgatcaa acgattgaag
    71881 ccattttttg tcataatatt ttgtgatttc aaatgagaaa cgatcgttcc aaaaaattga
    71941 ggtgccatag ccaaataata gacacggtta ccgtttaatt gatagtgtcc atctaatcgg
    72001 tcagacaatt cttttaatgt ctgataatgc gttgaatcat ttacatcatg ggattgatag
    72061 taaaaatggc ttgcaaaggt agttgcttct tcatctgaag gattcaatgc acggattgtt
    72121 tctttgacaa cttcacggta gtgctcatcg ctccagggac ggcgagcggt tccgattaca
    72181 gcaaaatgct cgcctaaatt tccttttctg taaaggcgga acaaagacgg gtacaattta
    72241 cgctgggcta ggtcacctgt accgccgaat atcgtaaaca atacattttt ttcattagac
    72301 atgtggatac actcctttat attttatcga caaaaatcgt gctggccgcc ttaaagctga
    72361 cggggatttt ctttctatca ttttggatag tgatcggtcc ttcataagct gcgatgtcaa
    72421 cgatctcata ttcttcgttg atattcaggt caatagtgac taagtaatcc aacaattctt
    72481 tttcatcaag cactcgtgcg atacgcactt ttgtaccgac aggataggac tccaatgttt
    72541 gtcggcgttc ttcatggaca acttcctctt ttttaggaat cgatcctcca tgaggacaat
    72601 gttcaggatg attcaaatag ttttctaaat gatctgcaag atgttccgaa gtcacatgtt
    72661 ccaatacttc agcatcatca tggacttcat tccagctata gtctaaatgt tcaactaaaa
    72721 aaacttccca taagcgatgt ttgcgaatca gactgctagc tttttgcagt ccgcttgcag
    72781 tcaattgtac accttgataa ggggaatgtt ctacaagctc ttctttcaca agtttagaaa
    72841 tcatttcact gacagatgca gctgagacat ctagactaga tacgatttgt ttattgttga
    72901 ctttcgttgt atctccacct agttctaaaa tgattttcaa ataatcttct cgatttggtg
    72961 tcatattcga tcatcctttc atggtaaaaa accatcatta gtaagtgtaa caaaaaaaac
    73021 gttaattgac tatttttcta aataaaaaac aaaggatttt tagacgtttt tacaacaaaa
    73081 aacgaaagat gatagagaag actcattctt tcgtttataa aggatatata tatacattgg
    73141 gagggatcta tttttcagat aacaacttat atgaagtgtc tgcagattcg tcataaagag
    73201 tttgttcggg ataagaaaag ataagataaa aaccatactg atcacgtacg actaaacgat
    73261 attcatctat ttctaccaca gatgtgccaa gagactgacc gttgatcaaa aacgtgtaat
    73321 cagatgtaat ctgtaattgt tgtttttgct ggctatcact aaagaaccat gtaccttgat
    73381 actttgagag tccttctgac ttttccgctt gttttttctt tttcaagtaa ataaaacttg
    73441 agagaacggt aatgagtgaa ccaagaaaat agatcatttt ttttatcacc aaatcactcc
    73501 tttatgagaa aataatcgca tagacatatt ctttcttctt tatttcattg taccgaataa
    73561 aagctaaaat ttctctaaga ctttcgactg gtttttttac tgtttttcta caaattatga
    73621 taggatacat agtaggaaga aaggtgacat aatttgaagt atcagcaagt ggaaatagct
    73681 ttttttattg accgcccgaa tcggtttata gcacattgta tgaaaaaaga cggagaaata
    73741 attattgcgc atgtcaaaaa tacaggtaga ggaaaagagg tcctgatacc aggcgctgaa
    73801 gtggctattg tatttgctcc tggacctaaa cggaaaaccg cctatgatct gattgctgta
    73861 aaaaagaagg gtgactggtt taatatcgat agtcaattgc ctaatcggct ggcaattgat
    73921 ggtattttag acggcacgat ccttttacca ggattaactg gtccatttac ggtatacaag
    73981 cgtgaagtaa cttatcttca ctccaaattt gatatttatt tagagacggt ggtcggacaa
    74041 aaagcatttg ttgaagtcaa aggtatgaca cttgaaaata aagcgattgg tgcttttcct
    74101 gatgcgccca cactcagagg attgaaacat ataggagaat taacgtatgc cgcacaagat
    74161 ggttatgccg cttatgtctt gtttattgct cagttcgaac atcttcatct ggcaacgatc
    74221 catgaagaaa tgcaaccagc actagctgat atggttcgac atgcgcagca aagcggagtt
    74281 caaatcctcg cctataattg tcaagtatcg gcagatgagg taactgtcaa gcaggccatt
    74341 ccatttgatg tagatatccc ttttgaagat cctatacaat agaaagaagg taaaataatg
    74401 atcaacttag gtattatcgg aacaaattgg attactcatc aatttgtcca agcagcttta
    74461 agcacaaaaa aatatgaatt gacagcggta tattctagac atttggaaaa agcacaggaa
    74521 tttggttctc attatgaagg ggatatcgaa ttcgcaacag atttgaagac ttttttcaat
    74581 attactcata tggacaccat ttatattgct tctccgaata gtcttcactt tagtcaagct
    74641 aagcaagcaa ttttggcagg aaaaaatgtg atagttgaaa agccagcttt ttcgacgcca
    74701 aaagaaatgg atgaaatcat ccatttggca aatgaacaca atgtgtattt ttttgaagca
    74761 gcaagaaata tccatgaatc aggttttaaa aagattgccg attacttacc attaaaagac
    74821 gagataatcg gtgcaaattt cagctatatg aaatattctt cgcgctatga ccaagtatta
    74881 gatggagaag aaccgaatat cttctcgact catttctcag gtggtgcatt gatggattta
    74941 ggtgtttacc ttgtatatgc agctgttgct tggtttggaa tgccaaaaga agtccattat
    75001 ttcccaagaa agatttctac tggtgtagat ggtcttggat gggggatctt acgttatgag
    75061 aattttgatg tatcgatcca gccaggtaaa atcggtgatt cttatttacc gtcagagatt
    75121 tattttgact caggaacgct agtgatgaat ggagtcaatg caattgaaca agcacaattt
    75181 catgatcgcg cgcataaaga aatcattgat ttggcggtaa cagctgcaga aaatccaatg
    75241 gcagatgaag cagaggattt tgccaatgtc atggctcatc cgaaagatcc agcttgggga
    75301 ttgttgtatg aagagtggat cgagctagct agaaatgtga accaagtgat ttatgattta
    75361 agaaaaaatg gcggcatcaa attcgatgca gataatgagg aagattttta atgaacgaat
    75421 ttgtacaagt atttcccgaa aattggcaga aaaaatatga ggataaaggg tttatctcac
    75481 cttctcttat tcaacgagca gtttttcagc cactaagtaa taaacaaagt atagtagcgg
    75541 tttctcctac aggttcaggt aaaacactgg cttatctttg gcctctacta ctaaacgtgg
    75601 aaccaggtga agcaagtgcg ttggtcattt ttgcttcttc tcaagaacta gctattcaag
    75661 tagcagatgt tgcacgtgaa tggggaaaag acaaagagtt gaaagtccaa tctttggtgg
    75721 gcggagccaa cgtcaaacgt cagattgagg gtttgaagga aaaaccagaa atcttgattg
    75781 gtacaccagg aagaatattg gaattgatga aagcaaaaaa aataaaagca catcaagtga
    75841 aaacaatggt atttgatgaa gcagaccagc tatttgacgc tgggaacagc caaatcattg
    75901 atcaaattct tcatcaagct ccaacagagt atcagctagc ttttttttca gcgacagcag
    75961 atcgttccct tgaaagcatt gaaaaaatca ctgggaaaac gtttgagaca attgacgtga
    76021 cagcagagga cgattctcga aaaggactga gacattactt tttacgtgta ccgatacgaa
    76081 agaaagatga ctacttgaga cgtctcacgc atatagaaga ttttcatggt ctgattttct
    76141 ttaatcaact gactgaatta ggcgtaatgg aagaaaaatt gctgtataga ggtgtaccgg
    76201 taggaagtct ggcctctgat cagaacaagc tactccgaaa agcagcgcta gaacaattca
    76261 aaaagaaaat ggtcagagca cttttgacca cagatgtggc ggctagaggt ttagatatta
    76321 caggtcttcc ttatgtagtg aatgcagaag ttccgttaag tgaagaagct tatttgcatc
    76381 gttcgggaag aactggtcga atgggtaatg aaggaatagt aattacactc gtacaagata
    76441 ataatctaag agatttaaaa cggcttttac gtccatcaaa tataacactt gaagagattt
    76501 ttttatacgg aggtaagctg caaactgagc agccaaccaa agaagatgca gctcctgcag
    76561 taaataaaaa gtatgcgaaa actttttctg gaaaggagca gaacccagaa tataacaaag
    76621 aaaaaataag aaaaaaagtt aaaaataaat caaaaaaaga gagaaataaa ggagcaaggc
    76681 gcaaataagc gcctttttat gctatgatta tgaaaacggg aacaaaattg gttgccagag
    76741 gaggggttat gtgaaagaac gtcaagaaga gatcttgcag ctattgacag aaaaccaaca
    76801 aggattgaca gcaagtgatg tagctgagcg cttaactatc gatcgaagca atgcaagccg
    76861 atatttaagt gaactataca aggcccatca tattgtaaaa accgctggcc gaccagtcgt
    76921 ttactctttg ccaacggaaa agtcaaaatc tgatgaagtg catgtcgact cttctacgca
    76981 agtgactttt gaaacactcg ttggagaaaa tgattcttta aaagtcagca tacagcaggc
    77041 aaaagctgcg attctttatc caccgcgggg gttgcatacg attatttttg gagaaactgg
    77101 tacagggaag tcgatgtttg ctgaatgtat gtatcatttt gcaatcgatt cagagatgct
    77161 ttctgcagat gcgccatttg tttctttcaa ttgtgcggat tatgctcaaa atccgcaact
    77221 gctctttgga cacatctttg ggatcaaaaa aggggcttac acaggagcag ctcaagatag
    77281 tcccggtcta atagccaaag cagacggcgg tatattgttt ttagatgaga tccaccgttt
    77341 accgccggaa gggcaggaaa tgctgtttac tttcattgat aaaggtattt accgtccgct
    77401 tggagaaagt gcacaagtac atgaagcatc tgttcagatc attggggcga cgactgaatc
    77461 ttcagaaagt ttcttatcta cgttcaatcg acggattcct atggcaatca ccttgccgaa
    77521 tcttgcttca cgttcactgg atgagcgtta tgaaatcatc tctcttttta tcaaacaaga
    77581 agcgaatcgt ttgaatcaac gtatcgatat cgaaaaagaa gcgattcttg ccttcatgct
    77641 ttatgatgcg gaaggaaata tcggtcaagt gaaacgagac ttgaaactag tctgtgcaaa
    77701 atctttttta cattaccgta ctcatcatga gaaaaagcta atcattcgaa aagaagaact
    77761 ttcccttcaa gtacaaaaag gattactgaa aatcaaggaa ataccggaac gtctcgaccg
    77821 attcatggac agcaaaagtc agtatttgac atttgaacca ggatttgctg atgttgtctg
    77881 gtcacaagat ccagaacgaa acatgcaggt ctataacgat attgaggaaa aagtcacttc
    77941 tctttccgaa accggtgtgg agaacattga tctagagacc ctcatttcaa aagatgtgga
    78001 tgcttatttc cagacatatg tcaaagaatt gactaaaagt tcgattccca aagatctttt
    78061 gccagatgaa atctggcggt taaccaatca attgtatgat gtggctgaaa aacggttaga
    78121 ccgtatttat aatgaaaaag ctcgttttgc ttttgctttg catctgcaaa gtacattgga
    78181 tcgtgtgaga gaaggtcata tgatcgtcca cccagatttg aataacgtac ggaaaaattt
    78241 taagagggaa ttccaggtag caatcgatct ttcaacgatc attgaagaag aagaacaagt
    78301 tgagattcct tttgatgaaa tcggctttat tagtatgttt ctttctatca atctaggaga
    78361 aagtgagcct gttaaagaaa acaaagtaga tgttctcata ttgatgcacg gcagacagac
    78421 tgcttcaagc atgttggaga tggcacaaga actattagga acaaaagtag ggaaagcttt
    78481 taatatgcca ctggttatgg aagttcaaac aatgtatgaa gaagtgttac aatatgtcaa
    78541 aaactatcaa gagcatttga cgaatggcct actcttgtta acagatatgg gatcattaaa
    78601 caccttcagt gatttgattt atgaagagac tggcattcgg acgaaagcta tcacgatgac
    78661 aagtaccatg attgtgatag aagcgatacg gatggcaagt gttggaagaa gcttagaaga
    78721 tatttatcaa aatatccagc tttcctttga atcgatcgta cgtgatcagt tcaaagcgga
    78781 aaagtctgaa actttacagc gtaaaaaagc cgtaatcgtg acatgcttta ctggtgaagg
    78841 agtagctgcc aaattatatc agcgtattat gccagtgatt aatcaaaata aggtagaact
    78901 gatccagatg cagtttttag aacgtgaatc attcaaaaaa cacatcgatg ccttattgga
    78961 agaatatgag atcaaagcaa ttgcgggaac agttgatgtt gattatcaaa atatcccatt
    79021 tttttcagcc tatgacgttt ttgatgatga aaaactaaat attttaaagc gtatagctga
    79081 tgatgagatc ccgacagcta aaattgtcga ttcacttgct ggatcattaa gcgatgtctt
    79141 ttctattgat gaactgatcc gttcattgca aaagacagtt cagcagatcc agaccgattt
    79201 gtcgattatc gtggaacctg gagtagatac tggaatcgtt atccatttag ccttcatgtt
    79261 agacggattg atcaaacaag aaagccatcg gcaatttgag aaactgtcta ttttctcaaa
    79321 aatttatcgt ttggaaatgg atatcgttcg ggcaaacttg atggcactag aacgaaaata
    79381 tcaggtaaaa attccagaag atgatatcgc atttcttaca caaatgtttc ttgaaaataa
    79441 aataaaaaaa tgaacacact aatcatactg tttacacact aaacatctgt gtgcgcacta
    79501 taaaaaagag ctaaaaagca tgaaaacgct atttcagctc ttttttttat tggcatgctt
    79561 cttgcgttat ataaaagtgt aaaaaaataa aaggagtgtt aaaaatggca aaaaaaacaa
    79621 tcatgttagt ttgttcagca gggatgagta cgagtttatt ggtcacaaaa atgcaaaaag
    79681 cagcagaaga aaaagggatg gaagccgata tttttgcggt ctctgcctca gatgcagata
    79741 ataatttaga atctaagaat gtcgatgtat tattattagg tcctcaagta cgtttcatga
    79801 aagctcaatt tgaacaaaaa cttgcgccta aaggtattcc attagatgtc atcaatatgc
    79861 aagactatgg catgatgaac ggagaaaaag ttcttgcaca agcagaaaat ctaatgaaat
    79921 aaatcaagta tgcagttggg ggtataacaa cgtggaagat caacaaaatc tagaagccat
    79981 catggggtta atcatgtttg gcggtaatgc aaagagcgat gcaatggaag cgattgcggc
    80041 tgctaaaatc ggtgattttg agttagcgga taggaaaatc gctgatgcag aagaatcact
    80101 tatacaagcg catcatgcac aaacagacat gttgacacaa gaagcacaag gaaatcatat
    80161 gcaagtgaca ttattgactg ttcacagtca agatcactta atgacatcta ttgcgtttac
    80221 tgatttagca aaagaaatca tcgaacttta tcgtcgcgta tacagcgaat aaatacaact
    80281 tcttttttga acggaagaag aaaaattttt gaacggattt tttattttgt tataaggaga
    80341 actctcttta tataaaaaac ttataggagg aagtaaaaaa tgaacggact atcaagttgg
    80401 atggagaaat acatcctgcc ggttgccgca aaaatcggag cgcaaaaaca tttagttgct
    80461 ttgcgtgatg cttttatcgg cacactgccc gcaacaatgg cgggatcagt cgctgtaatg
    80521 atcaatgcaa ttattcgtga tttaccgcaa cagtttaaca gtacctatgc tgacagttta
    80581 gcagcagaca aaggaatctt tgccgtattg aatgtcatca tcggaatcaa tggttttgtc
    80641 tggaacggta cactagcaat cgccggattg atctttgcct tttcttgggg gtataatctt
    80701 gcccgagctt atggagtaaa tgatctagct ggggggatcg ttggtttagc taccttgatt
    80761 caaggtattt cctttggtcc agcattatca gcagcattag atgtgaatgt tccttcaaat
    80821 gtcgcagatg caatcaatag ttctgagatt ggtgcaacga ttgcagatgg cacactgtca
    80881 gttggattat ggggttggct aaaactagat catttgaatg gaaacgctta ttttacagtt
    80941 atgatcatgg gagctatttc agtcattatt ttttgtaaat tgatgcttgc caacattacg
    81001 atcaaaatgc ctgattcagt accaccagca gtatctaaag catttgcagc cattttacca
    81061 gcgacgatcg ctttatacgt cattgctatt atcaattata tcgtaggtcg tgtagctgat
    81121 gggcaactga tcattgattt ggtgcaaaaa tatattgcag agccattcct aggattatct
    81181 caagggttgg gagcagtatt gatcgtcaca gtatttgttc aagtattctg gttctttggt
    81241 attcatggac caaatgtatt ggcaccagtg ctagagggaa tctggggaca agcacaacta
    81301 atcaatattg atattttcca aaaaggttat aacggaaaaa ctggtacacc agccgtgtta
    81361 gatgcaatcg aagatggaaa agcttatatg tgggtccgcg gatcgttcga tgcttttgca
    81421 tggtttggcg gatcaggcgg aacgatcgtc ttactgatag ctatcttgat tttctctaaa
    81481 cgtgcagatt atctaacagt agctaaatta tccattgggc ctggtatctt caatatcaac
    81541 gaaccagtga tgtttggtct gccaatcgta ttgaatgcaa tcatgtttat cccattcttg
    81601 attgctccag tagtagcaac agcaattgga tggacagcta cttatcttgg cctagtcgct
    81661 ccggtatcgc agcaagtcac atgggtagta ccgccgttct tattgtcatt cttagcaaca
    81721 ggagcggatt ggcgggcacc gatcgtaaca ttagtatgta tggtcgtgac tttcttgatt
    81781 tgggcaccat ttgtcatctc tgcaaacaaa atggaccctg ccgcaggcga acaacaataa
    81841 gatgaacgtg aggagcggga cgctttaagt ttcgttcctc ataatgaatt ttcgaggagg
    81901 aactgaaatg attacaattg ccagtgcaat gcaacaagat gaaatcaaag aattgttaga
    81961 aggatacaat gaagaaggaa tgacttttac atttaaagaa aaacaaggaa tcaagctgtt
    82021 ctttgaaaca agtgcagaag atccggaagt tgcggctaaa aaagcaaagg aactaatcaa
    82081 agcacagcct tggggaacag tcctttattt tcaatcaaca gctgtttaga aaatggttta
    82141 aaaagatagg atggttgaga tgataaaaaa cggtttattt ttatgtagta ttggttcttt
    82201 gatcgtgttg tatagtttga atccacattc gcaaaggtat gatttgtata gtatggtcac
    82261 aggtcttttt ctaacaggat taggaatcta tttcttcatg aaggggaaaa aaagagaaga
    82321 agcaaaggag aaaaataact aatggaaatc acgaaaaaat taaatattcc agcatcgttt
    82381 ttttacgata aagttatgga ctctgtcctt ttcgatgtac gtaaagcgac cggcaaatca
    82441 gtaactagaa aacagctgaa aaatttggag tacgtcaaac aattctcgca aaataaccgg
    82501 gctcgcataa aaatcgaaga agtcattgaa aaccagtctt ataagtttcg tacatcgaca
    82561 acgaagaatg aattcgtcgt tcattatcaa atcaaaccat tggacgattc gacctgtgag
    82621 gttcattaca cagaaaaaat ggagtcttat ggcatgcttc aaaagttgaa tgatatgctt
    82681 ttaggaacaa tcttgatgta ttttaaacgg agacgtttca aaaaaatgct agatatgatc
    82741 gaagcaacgt attaaatcaa tgaaacatgg tactatttag ttattgaaaa atgaagtaag
    82801 ctagaaggaa tttttcatag gaagatagat ctttcctgcg aatcctcact agcttttttg
    82861 taggaagggg cagcaaaatg acaaacaaag taagagagct aggtgtatct tcgattgagg
    82921 aaatgtttga tttagccact tatgcattta atggggcaga tacacctgaa aggcgagaac
    82981 gttttcgtac acttgcggag aattcttgga attacggatt ttttgatgag gaggatcggc
    83041 taacaagtca agtcatggcg accccttttc cagtgaattt ttacggacaa gagtacctaa
    83101 tggccggcat tggctatgtt gcttcttatc cagaagcaag gggacaaggc gggatcaatc
    83161 agatcatgga aaagatcctt gaagattgcc gtgatcgtaa agtaagctta tcttatctgg
    83221 cgccattctc ctatcccttc tatcggcgct atggctatga acaatcattt gataaaatca
    83281 gttatcaact aaacagtcgt gatgtgccgt ttatcaaaaa aaaatctgga aaaataaagc
    83341 gtatggattt tgaagatgca aaagctgcaa tcagaagaat ttatgaacag atgccggaaa
    83401 accaacgagc tggtctgaag cgcgaagact ggtggtatac gtataaattc aagcaaaaag
    83461 gcaattacca gtttgctttg cattttgatg ataatgagga agcaacggga tatatcgttt
    83521 atcagcttgc aacgtcttcc tttattattg cggagtgggg atttttggat catgatgctt
    83581 tttgcagcct tgttcgcttc gtaagttctc acaatggtgc ttttgaaaca tttgaatatt
    83641 catgcggcta cggcggtaat gatcaaaatt atctattaga aaatccattt gcttctgtaa
    83701 tgatcacacc ttatatgatg gcgaggatcg tagatatttc tttgtttttg gaaaattatc
    83761 catttaaaaa gagacaagct gcatttgccc tacaaataga agaagatcat tatgcgaaat
    83821 ggaatgaagg aatcgtggaa gtaacaatcg aaaatggaaa aaatcatctc tcaaaagtag
    83881 aacaaacaaa actcccaatc gttcgaggaa gtatccaaca tatcacgcag ctattattag
    83941 ggtatcgtaa agtggaagag ttggtattcc aagaaagaat acaggtgaca aaagaatatg
    84001 aagaaacgtt aagctcaatc ttgccacagc aacagccaat tcttaatgac tatttttgat
    84061 aaaaaacggg tgatgacaat atttttgtca ccaccctttt gtttttcatt tggcatttga
    84121 aggaaaatgt taaagtaaat cctctttttt cgtaaatttg aattttttgt tttcttttgt
    84181 tttagggttt tcttttttgt ggtcttggat tgcttctttc gtaatctttt cccgattttt
    84241 tttagatgaa tctaagcgtt ctgcgtatac tttttctttt ggcttatcat tcattggaaa
    84301 ggacctccct acattatttc attctacctc tagtttatca tctgttaagg actagtggaa
    84361 acgttttgcc taacttataa gctgattttt ttagcagata agcttgcaag taaaaaacaa
    84421 acaagctaca attgatttat tgaaattgtt cctgtagctc agtaggatag agcaatcgcc
    84481 tcctaagcga taggtcgctg gttcaattcc agtcaggaac gtataaataa cccctgtatc
    84541 ctttgcggta caggggtttg ttttgtattt gtgccaaaaa tgtgccaaac tagttatcat
    84601 ttttatataa actatcgaac acagtattga ttttttcttt atcctgatct tcaagctctc
    84661 gaacaatatg aagataagta gacattgtag tttctaaact gctatgtcct aaacgtttcg
    84721 atacgctaag tatatttact ccttgataaa gtaaaataga tgcatgtgta tgccttagtc
    84781 catgcaaagt aaattgcttt tcaatcccta attcttttaa ttttttgcgc aaatatttgc
    84841 tgacggcgtt tgatgaaact aatccatttt ttaagttgaa aaaaacaaaa ttatgagggt
    84901 ttttgatttc aaaattctcg aataattctt tttgattgat tttaaacttt tttaagagat
    84961 caatcgtatg tttatcaatg gaaattttcc tatttgaagt ttcgtttttt gtttttcccc
    85021 attcatttaa tttataattc catgttttat taatagtgat gatttgatct tcgaaatcga
    85081 tatcttccca tgttaatcct agaagttcag caaatcgcat tccagtggca ccagcaacta
    85141 acacgagcat aggagaagag tactgcgcag aaaggttttc ttctacgagt ttcattaaag
    85201 atttaaattg atcataatct aaatatttat cttcttttgg cttcaatgaa tcttttcctt
    85261 tgattactgc ttttctagtt ggatcaaacg gaattaatcc ttcttctacg gcatctttca
    85321 acgacgctct aatatggtta ttaaacttaa taacggtaga ttttacatgg tctttagcat
    85381 acttatttaa aaagcgttga tatcccactc tatctaaatc agaaatcaat accgctggca
    85441 tatatttctt tatgttcatt aacgtatctt cgtattttct ataagtgata ggagatactg
    85501 ttccttcttt ataaagttgc atccaatcct caaagtaatc tgataataat agatttttgc
    85561 gatccccttt aaggcctttg ttcaagttat attctaattc gttggcggca tcttttgctt
    85621 ctccttttgt cttaaatcct gattttctta tcttcgtgta ttttccgtca tcttttttgt
    85681 atgaaatttc gtattgccaa ctatttccac gtttgactaa tcttgccata attgaatcag
    85741 ctctctttct ctgatacaat aggaactata aagaagccca ttgtttgggt tttattattt
    85801 tttgcacatc ctttttgctt gccggcgcaa ggatgtgcta tttgtcgttt taatggtata
    85861 attggatgtg tattagatca tccaatcaga ggtgtttatt atgattaaag tgatttttaa
    85921 aagaataact gaacttccag agtttagatt atttgttgat tattggtatg tctggttttt
    85981 tattgttgca ttgctgatag gtctagatgt catattagaa agaaaactta agtaagagtt
    86041 gagttaataa attgaattcc taaatgagat attttgtagc ccatattttg tttgccgaca
    86101 tcacctaata ctatggagct aatcgttttt tctaacagac caaaatcaga taatctattt
    86161 aagctaccat ctattagctc ttgtgaatag ccagacatgc tgatatttcc agtgaaattt
    86221 tcgtttccac gactcattaa gcatgctaac aattgaatct caatcgatgt taattgattc
    86281 agtacaagaa gataatactc ctcgttatcc catgaagcgc tgttaattcg aagtaaacaa
    86341 ttcttataaa gatttttata catatctatt ttattttgtg cctttgattt ttctacctcg
    86401 tcatttatcc cttcgaatat tccaatgagt tggtccttgt tctctaagtt gaaaacatct
    86461 tctggaattt gatcgcgaat gttttctaat gattcgttta aatttttgta gaatttctcg
    86521 atgcgtttga aacgcttctc gtttttagac ccaaaataaa gtgactgcac aacgccacca
    86581 atataaggaa ttgctccaat accgccttca actattaatt caagatattc ttttttggtt
    86641 agtttttcat tgctattatc catagttacc tcctattttg tcatttcctt tacaagcatt
    86701 gtgactcctg atggcactct tttgtatagg ttttattatt gtgcacaatc taactcttag
    86761 tggggcgtgg ttcatttatt ttttacttac tttactatta atcatatccc attgagcata
    86821 ggatttttct atgttcgatc ctcttgaact aatttctcct gtaaaagtta ctttatcgcc
    86881 ctttgtaaag gtatcagcat tgtttaagtc cttcgcaaaa attacatatg aaactttagg
    86941 tgttgcagaa atatcattcc aacttttatc atcgtactct ttgtctgcta taatggctat
    87001 tctagtagtc attgattcga taacagttcc ttcaaaagta tatgttttac cactaatata
    87061 tttagtaaaa gctgtatttt gctcagtagc tggagtaata gaataataac tatctaagaa
    87121 agcaccccag tcatgattat ttgtctctaa aaattcttca aattgagtta ggcttttatt
    87181 gctgctgtta gatgtagtag tttgagtatc agatgatgag ctttcctgtt tgttattgtc
    87241 atttgcctcg tttgatgagg aaagcgagaa acctacggta gtaaaaataa tcccgataat
    87301 aaggagaatc ataggtgttt tcttatttgt ttttttgata aatcttacaa ttaacatgat
    87361 taaacctata ataaataaaa caataccagt aaaaccaaat ataccgtcca ttaatagatc
    87421 ctctttctct tttgatattg aatattataa accaagcaat tcttttttct taaaatcgaa
    87481 ttcttcttga ttaataatcc cttcatctaa caactcttta tactttttaa tttcatctgc
    87541 acttgaaatt tgtgtttctt tggaattatt atctaatata gattgttgct tctctatata
    87601 tttttttaaa ttttccattt cattccaata tttttttgta aacataacgg tgttttcatc
    87661 tttcgtggcc gctaatatac ctccgcgact ttcgtttccg cctagcaagg taaattgtat
    87721 ataaccatta ctcatacctg gtttttttaa ttgtacggct gatatatttt taaaggggat
    87781 agttttttct cctttaatac cttgattaac taaatttata aatccttttc taataattga
    87841 aatagaatca tcagttactt taatcaaggt ttttcctgga gattttatta aaatctcttt
    87901 ttccattttt tcttcctcat ttctgtgata tgattttttt tgtagaagct catatatgag
    87961 gaaagagccc gtgtt
//
LOCUS       DACDXE010000002        82998 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.002, whole genome shotgun sequence.
ACCESSION   DACDXE010000002 DACDXE010000000
VERSION     DACDXE010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 82998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.002"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..561)
                     /locus_tag="IWM23_000092"
     CDS             complement(<1..561)
                     /locus_tag="IWM23_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HAP9882771.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(748..1398)
                     /locus_tag="IWM23_000093"
     CDS             complement(748..1398)
                     /locus_tag="IWM23_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882772.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWM23_000094"
     CDS             complement(1941..2876)
                     /gene="whiA"
                     /locus_tag="IWM23_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAP9882773.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2910..3908)
                     /locus_tag="IWM23_000095"
     CDS             complement(2910..3908)
                     /locus_tag="IWM23_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAP9882774.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWM23_000096"
     CDS             complement(3905..4789)
                     /gene="rapZ"
                     /locus_tag="IWM23_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAP9882775.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4924..5655)
                     /locus_tag="IWM23_000097"
     CDS             complement(4924..5655)
                     /locus_tag="IWM23_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAP9882776.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5659..6924)
                     /locus_tag="IWM23_000098"
     CDS             complement(5659..6924)
                     /locus_tag="IWM23_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAP9882777.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWM23_000099"
     CDS             complement(7187..10003)
                     /gene="uvrA"
                     /locus_tag="IWM23_000099"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAP9882778.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWM23_000100"
     CDS             complement(10013..12007)
                     /gene="uvrB"
                     /locus_tag="IWM23_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAP9882779.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12266..13768
                     /locus_tag="IWM23_000101"
     CDS             12266..13768
                     /locus_tag="IWM23_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9882780.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13770..14507
                     /locus_tag="IWM23_000102"
     CDS             13770..14507
                     /locus_tag="IWM23_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9882781.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14552..14725)
                     /locus_tag="IWM23_000103"
     CDS             complement(14552..14725)
                     /locus_tag="IWM23_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9882782.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14701..15366
                     /locus_tag="IWM23_000104"
     CDS             14701..15366
                     /locus_tag="IWM23_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar diacid utilization regulator"
                     /protein_id="HAP9882783.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWM23_000105"
     CDS             complement(15448..16614)
                     /gene="dnaJ"
                     /locus_tag="IWM23_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAP9882784.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWM23_000106"
     CDS             complement(16765..18594)
                     /gene="dnaK"
                     /locus_tag="IWM23_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAP9882785.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWM23_000107"
     CDS             complement(18645..19208)
                     /gene="grpE"
                     /locus_tag="IWM23_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAP9882786.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWM23_000108"
     CDS             complement(19302..20345)
                     /gene="hrcA"
                     /locus_tag="IWM23_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAP9882787.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20579..21745)
                     /locus_tag="IWM23_000109"
     CDS             complement(20579..21745)
                     /locus_tag="IWM23_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAP9882788.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21761..21988)
                     /locus_tag="IWM23_000110"
     CDS             complement(21761..21988)
                     /locus_tag="IWM23_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAP9882789.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22472..23062
                     /locus_tag="IWM23_000111"
     CDS             22472..23062
                     /locus_tag="IWM23_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAP9882790.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23252..23965
                     /locus_tag="IWM23_000112"
     CDS             23252..23965
                     /locus_tag="IWM23_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882791.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24080..25090)
                     /locus_tag="IWM23_000113"
     CDS             complement(24080..25090)
                     /locus_tag="IWM23_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAP9882792.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25127..25609)
                     /locus_tag="IWM23_000114"
     CDS             complement(25127..25609)
                     /locus_tag="IWM23_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAP9882793.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25759..26127
                     /locus_tag="IWM23_000115"
     CDS             25759..26127
                     /locus_tag="IWM23_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAP9882794.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26592..27044
                     /locus_tag="IWM23_000116"
     CDS             26592..27044
                     /locus_tag="IWM23_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9882795.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27168..28019)
                     /locus_tag="IWM23_000117"
     CDS             complement(27168..28019)
                     /locus_tag="IWM23_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAP9882796.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28032..28817)
                     /locus_tag="IWM23_000118"
     CDS             complement(28032..28817)
                     /locus_tag="IWM23_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9882797.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWM23_000119"
     CDS             complement(29169..30110)
                     /gene="ribF"
                     /locus_tag="IWM23_000119"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAP9882798.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWM23_000120"
     CDS             complement(30114..31037)
                     /gene="truB"
                     /locus_tag="IWM23_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAP9882799.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31405..33767)
                     /locus_tag="IWM23_000121"
                     /pseudo
     CDS             complement(31405..33767)
                     /locus_tag="IWM23_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34215..34388
                     /locus_tag="IWM23_000122"
     CDS             34215..34388
                     /locus_tag="IWM23_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9882800.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWM23_000123"
     CDS             complement(34545..34892)
                     /gene="rbfA"
                     /locus_tag="IWM23_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAP9882801.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWM23_000124"
     CDS             complement(34919..37225)
                     /gene="infB"
                     /locus_tag="IWM23_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAP9882802.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37238..37549)
                     /locus_tag="IWM23_000125"
     CDS             complement(37238..37549)
                     /locus_tag="IWM23_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAP9882803.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37546..37839)
                     /locus_tag="IWM23_000126"
     CDS             complement(37546..37839)
                     /locus_tag="IWM23_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAP9882804.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWM23_000127"
     CDS             complement(37861..39036)
                     /gene="nusA"
                     /locus_tag="IWM23_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAP9882805.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWM23_000128"
     CDS             complement(39059..39532)
                     /gene="rimP"
                     /locus_tag="IWM23_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAP9882806.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39677..44035)
                     /locus_tag="IWM23_000129"
     CDS             complement(39677..44035)
                     /locus_tag="IWM23_000129"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAP9882807.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44247..45956)
                     /locus_tag="IWM23_000130"
     CDS             complement(44247..45956)
                     /locus_tag="IWM23_000130"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAP9882808.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWM23_000131"
     CDS             complement(46024..47292)
                     /gene="rseP"
                     /locus_tag="IWM23_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAP9882809.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47453..48253)
                     /locus_tag="IWM23_000132"
     CDS             complement(47453..48253)
                     /locus_tag="IWM23_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAP9882810.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48250..49062)
                     /locus_tag="IWM23_000133"
     CDS             complement(48250..49062)
                     /locus_tag="IWM23_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAP9882811.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWM23_000134"
     CDS             complement(49257..49814)
                     /gene="frr"
                     /locus_tag="IWM23_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAP9882812.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49817..50539)
                     /locus_tag="IWM23_000135"
     CDS             complement(49817..50539)
                     /locus_tag="IWM23_000135"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAP9882813.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50675..51556)
                     /locus_tag="IWM23_000136"
     CDS             complement(50675..51556)
                     /locus_tag="IWM23_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAP9882814.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWM23_000137"
     CDS             complement(51655..52437)
                     /gene="rpsB"
                     /locus_tag="IWM23_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAP9882815.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52796..53275
                     /locus_tag="IWM23_000138"
     CDS             52796..53275
                     /locus_tag="IWM23_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAP9882816.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53492..54583
                     /gene="phnW"
                     /locus_tag="IWM23_000139"
     CDS             53492..54583
                     /gene="phnW"
                     /locus_tag="IWM23_000139"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAP9882817.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54576..54704
                     /locus_tag="IWM23_000140"
     CDS             54576..54704
                     /locus_tag="IWM23_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9882818.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54708..55253
                     /locus_tag="IWM23_000141"
     CDS             54708..55253
                     /locus_tag="IWM23_000141"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAP9882819.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55707..57398)
                     /locus_tag="IWM23_000142"
     CDS             complement(55707..57398)
                     /locus_tag="IWM23_000142"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAP9882820.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWM23_000143"
     CDS             complement(57818..58765)
                     /gene="arcC"
                     /locus_tag="IWM23_000143"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAP9882821.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWM23_000144"
     CDS             complement(58880..59899)
                     /gene="argF"
                     /locus_tag="IWM23_000144"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAP9882822.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWM23_000145"
     CDS             complement(59990..61219)
                     /gene="arcA"
                     /locus_tag="IWM23_000145"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAP9882823.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61680..62381
                     /locus_tag="IWM23_000146"
     CDS             61680..62381
                     /locus_tag="IWM23_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9882824.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62990..64006)
                     /gene="tsaD"
                     /locus_tag="IWM23_000147"
     CDS             complement(62990..64006)
                     /gene="tsaD"
                     /locus_tag="IWM23_000147"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAP9882825.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64003..64467)
                     /gene="rimI"
                     /locus_tag="IWM23_000148"
     CDS             complement(64003..64467)
                     /gene="rimI"
                     /locus_tag="IWM23_000148"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9882826.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64474..65016)
                     /gene="rimI"
                     /locus_tag="IWM23_000149"
     CDS             complement(64474..65016)
                     /gene="rimI"
                     /locus_tag="IWM23_000149"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAP9882827.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65000..65824)
                     /gene="tsaB"
                     /locus_tag="IWM23_000150"
     CDS             complement(65000..65824)
                     /gene="tsaB"
                     /locus_tag="IWM23_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAP9882828.1"
                     /translation="MYYCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDNIIVPMFDARRKNVYAGAY
                     RWKENRLETVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFSEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(65913..66893)
                     /locus_tag="IWM23_000151"
     CDS             complement(65913..66893)
                     /locus_tag="IWM23_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9882829.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66917..68401)
                     /gene="galT"
                     /locus_tag="IWM23_000152"
     CDS             complement(66917..68401)
                     /gene="galT"
                     /locus_tag="IWM23_000152"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAP9882830.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68413..69402)
                     /gene="galE"
                     /locus_tag="IWM23_000153"
     CDS             complement(68413..69402)
                     /gene="galE"
                     /locus_tag="IWM23_000153"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAP9882831.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69650..69817
                     /locus_tag="IWM23_000154"
     CDS             69650..69817
                     /locus_tag="IWM23_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAP9882832.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69879..71690)
                     /locus_tag="IWM23_000155"
     CDS             complement(69879..71690)
                     /locus_tag="IWM23_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAP9882833.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71687..72052)
                     /locus_tag="IWM23_000156"
     CDS             complement(71687..72052)
                     /locus_tag="IWM23_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAP9882834.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72215..72610)
                     /locus_tag="IWM23_000157"
     CDS             complement(72215..72610)
                     /locus_tag="IWM23_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9882835.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72628..73590)
                     /locus_tag="IWM23_000158"
     CDS             complement(72628..73590)
                     /locus_tag="IWM23_000158"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAP9882836.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73590..73802)
                     /locus_tag="IWM23_000159"
     CDS             complement(73590..73802)
                     /locus_tag="IWM23_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAP9882837.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73823..74521)
                     /locus_tag="IWM23_000160"
     CDS             complement(73823..74521)
                     /locus_tag="IWM23_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAP9882838.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74541..75083)
                     /locus_tag="IWM23_000161"
     CDS             complement(74541..75083)
                     /locus_tag="IWM23_000161"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAP9882839.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75215..76219)
                     /locus_tag="IWM23_000162"
     CDS             complement(75215..76219)
                     /locus_tag="IWM23_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9882840.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76216..77205)
                     /locus_tag="IWM23_000163"
     CDS             complement(76216..77205)
                     /locus_tag="IWM23_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAP9882841.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77202..78008)
                     /locus_tag="IWM23_000164"
     CDS             complement(77202..78008)
                     /locus_tag="IWM23_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9882842.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78174..79130
                     /locus_tag="IWM23_000165"
     CDS             78174..79130
                     /locus_tag="IWM23_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9882843.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79207..79725)
                     /locus_tag="IWM23_000166"
     CDS             complement(79207..79725)
                     /locus_tag="IWM23_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9882844.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79813..79962)
                     /gene="rpmG"
                     /locus_tag="IWM23_000167"
     CDS             complement(79813..79962)
                     /gene="rpmG"
                     /locus_tag="IWM23_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9882845.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80190..80636
                     /locus_tag="IWM23_000168"
     CDS             80190..80636
                     /locus_tag="IWM23_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAP9882846.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80831..82726)
                     /gene="asnB"
                     /locus_tag="IWM23_000169"
     CDS             complement(80831..82726)
                     /gene="asnB"
                     /locus_tag="IWM23_000169"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAP9882847.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23581 a        17672 c        14335 g        27410 t
ORIGIN      
        1 tataaacatc accccaaata aaacagaaaa cagtataagc attataaaaa ttatcaacaa
       61 ccttgatcct tcttttatag attcaggaaa cagtataatt ttagtactag ataaattgtt
      121 tacaccgatt agtgttaaaa tatctttatt cttcttgcta acataaccct ttatgcacaa
      181 aagaacagct atacttaaag taacaaataa ataacgtatt ccaatagaaa aatcactata
      241 aatactctct agcataggac cgacaattga agaagtcact actgcaaatg ctggacctat
      301 ccaagcaaca ttagaatgtt ctttctctct tttcctttgt aaatctttgt tgagtattaa
      361 tctatttatt tcttctgcag gcaagttaat tttcataacc ttatggggga ttatccaatt
      421 aaaaatcgga aaaaggtagc ctccccaaaa agtatagtga tctgtgtcaa tcagataata
      481 acctgatttt gattccagaa taacatatct caaagccgaa cgaaaatttt tcatgaatat
      541 ccataatctc actgtttcca cacctaattc ctccaattcc aattaaatgg atttatagca
      601 tcaccaatta tgttaactgc ctgaccaacc tcattgataa aattggcacc actatcccat
      661 gcttcaacaa tgctatatat ctatcaaaca aaaaatggaa aactaaatac tcgcgcacat
      721 aaattttaca ttctgttttt tataagacta ataataatca ttgaaaaaat ttcttatcgg
      781 tggcgcatat tcccaattga ttaatgtgat cccatattct gcaagtattt tacgagcttc
      841 ttctaagtca tagtgagaaa aatgcataaa acctgcaata cgatcacgga ctgtgagctg
      901 tggcttgctt ccaccatcat tcataaaata tccagcagag ccactactta aagtgattgt
      961 tacatcttca ctttctatta ttaagaattc ataatgaaat gctttcaact ctccactatt
     1021 atttaaaagt tttatactcc aacttcgtct aggttgagtt gtcacattcc ctctaaaact
     1081 tcctataaat tctgcaatca ccgtatcttc aatcaaaatt ctttcctcat ctatttcata
     1141 ttcaagacta aacggaaatt ctccatatgt aatctctggc aatggtggtg ctgtagttct
     1201 ccaaataaat acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag
     1261 acctgctcga agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag
     1321 aaaaataaaa agtactgcaa tcataatgat agtaaaagtt gtaaaaaagc acattttttg
     1381 ccctcccttt ataaacatcg attctcaaat tctaaaccaa atcttgatag ttcaagttca
     1441 ggtataacgt ataccaacca tacatcccaa ttactattta tgaacttatg attatcactg
     1501 aataaatcac tccatcccct tgttaaaact tctacattaa ttacacctgg ttgttttcga
     1561 ctagctaatc caatgaactt atcaaccatc cttatccacc aaccacgaga atcttgacca
     1621 gaagtaagaa aaacagttct caaggttgat cgagcagttc cttccagttt ttaattaatc
     1681 tccagttata gataaatact tgatcctatc tatatcagga cttctatatt aagcaaaagt
     1741 ttatgtataa aactaaaatc ttcattcagt tgtctccccg ctttttatac gattgtatta
     1801 aaaaacaata gaagcctttc aacattatgt tactccagta aagttggaat tatagtttga
     1861 cggaagaaaa gcaagtatag cttttaaaaa aaactgatac attcgcatct cctgcttcat
     1921 gtatcagttt tttctttgga ttaaactgct tcttcacgca gtttttccgc aaattcattt
     1981 attttgcgaa ttcggtgatt aattcccgat tttgatattg cacctgaggg aatcatttcc
     2041 cctaattctt tcaagctgac ctctggatgt tgaagccgaa gttctgcgat ttcctgaagt
     2101 ttttcaggaa gggcatgtag accgaccttt gcttcgatca attcgatgtt ttcgatttgt
     2161 ttagaagccg catcaatcgt cttgttcaga tttgctgtct cacaattgac tagacgattg
     2221 accgaatttc tcatatcccg tacgattcga acatcttcaa atcggagcat tgagttggtt
     2281 gcaccaatca atgttaagaa atcagcaatt ttttctgctc cttttaaata cgaaatatag
     2341 ccattgcgcc gttcaagcgt tcgagcatgt agaccgtaat aatttaacat atcgcaaatg
     2401 tcttggttat gctgttcata aatagaaaat atttccaaat gataacgact cgtttccgga
     2461 ttattgactg aacctgaggc cataaaagca ccacgtaagt aagaacgcat tttttgcgaa
     2521 tttcccataa tctcgtccga aacgtgtcca ttgaacatca taccatccat gatatcaaga
     2581 tccatcaaca catcttttgt gccttgcttc aaccggacga tatagacatt gttctttttt
     2641 aatttcattt ttttacgaac gagtagctca cttcgtacct gataatgatc tttcaaaaga
     2701 gaatagatac gtctggcaat tgcagcattt tctgtttgga cgttcaaaat aaactgctgg
     2761 ttcacaatac ctaaagagcc gttcattcgc agcaaagcag ctaattccgc tttcgcatgt
     2821 tctcgatgaa cttccaaacc agttagttct ttcttcacat ctgcagcaaa tgacatccta
     2881 ttctcctcct ttctccaaca ataaattgct taatattttg aaccgaaaac taaacggaac
     2941 aattcttcta ctactttgtc tccatcgtgg aagacaccac catctttcaa ttctaaaaag
     3001 tcggtggaaa tcactcgaca tccttcattt ctcaatccgt tgaagtcgtg tgcgacttgt
     3061 actagatact catcatagat ctctgggtcc atataatctt caggcacttt ttccgtattg
     3121 accaatactg tatcgataaa aggctgattc aaatgcttgt gtaaaacacg tatatgatcg
     3181 gcatctgtga aatgttccgt ttcccctttt tgggtcatga tattacagat atagacggtt
     3241 tcagcagttg tcttgagcat tgcttcgccg atttctggta tcactaaatt tggaaggata
     3301 ctggtgaata aacttccggg tcccaaaacg accatgtcgg cgtccataat tgctgaaacg
     3361 acttttcgag ccgctttggc ttcactttta tcatttgtgt tgcagacata cacacggtca
     3421 atggttttac ggttgatcgc gattttagat tctcctactg cagtcgttcc gtctttaaaa
     3481 acagcatgca aggtcaaagg agtttctgaa gaaggataaa catgtccatc tacatgcatc
     3541 atttttgcta ataattgaat cgcctcataa gtgcttcccc gcatctcgga aactgcggca
     3601 atgatcagat taccgatcgt atgattggct aaaaactgat cttccttttt gaaacggtat
     3661 tggaaaatat cttcatagaa ttgtggcata tccgataacg ccactaagac atttctcaag
     3721 tcaccaggag gagccatagc gatcgattct ctgattgcac cgctgcttcc accatcatct
     3781 gcaacagtca ctactgctgt gatatctgct ccttgatttc gtaaactttt caggataact
     3841 ggcagaccag tgccaccgcc gaccactacg atctttggtt ttcttatccg gtaggttttc
     3901 attttcacga ccggttcacc gtctctttcc ttttatcctt gtcccgatgt gtcacattga
     3961 ctttatagtt tttccctaaa gcatttgcca aacgttcagt caatgccaca gaacggtgtt
     4021 gaccgcctgt acagccgatt gcaactgtca agctgctttt tccttcttta atgtatccag
     4081 gcaatacagt ttccagaaga tccaaaaatt tatggtagaa tgattgcgtt tcatcaaagg
     4141 acatcacata atcatagact ggctgatctt ttccggtcaa tggtcttagt tccgggatat
     4201 aatgagggtt gggcaaaaaa cgcacatcca tcacaatatc cgaatcgatc ggcaacccgt
     4261 atttaaaacc aaatgataca aattcgatcc gaaatccttg gtctcccttt gatttgaatg
     4321 actcattgat tttttcacgt aattgtcgtg gtgtcaatgt tgtcgtatca atgatcacgg
     4381 aagcttgggc tttcaagtcg tctagtatcg cccgttcttt acgtatccct tcagtgatca
     4441 aaccgtccat cgccattgga tgtgctcgtc ttgtttcttt gtagcgagcc actagttctt
     4501 cgtctgaagc atctaaaaat aagatagaag tgtcgatgaa gttcgtattt tcgatttcga
     4561 tcagcatgtt ttggatttct tcaaagaagg aacgagaacg caaatccact cctaaagcca
     4621 tttttgtgac tttacctgat tctttgatca attcccagaa ttttggaatc aatcgaggcg
     4681 gcatattatc gatacaaaaa tagcccatat cttcaaaact ttggatagct actgttttcc
     4741 ctgcaccact catacctgtt atgatgacta attgcaaatt atctgtcata tttacgaacc
     4801 cctctcacat tgtgcataat aaaattatat catcccgggc gtatcattac cagttttctt
     4861 tttcttttac aaaaagattt ggaccaaata tcttttagga tatttgcccc aaatccaatt
     4921 tttttacttt tccgatgctg tatccaatgc ctcgcttctt tcagctaatg cacgttcgat
     4981 cgttcgatcc gctaaaatag attgagcatc gatcactggg taatgattat cttcagcaaa
     5041 ttcatgcatc aaagataatt ctgtgcaacc taggatgact ttttcacagt ttaggcgctc
     5101 taccgcttcc tctaagattt cgtaatagag ctcttgattc aaataatccg attccttgat
     5161 ctcatgatag atcaggtaat tgattttttc ttgaagcgca gtatcaggaa tcactgtttc
     5221 gaaaccaaga tttttgactt cgcgttcata gattccagct ttcatacttc cttctgttcc
     5281 tagaatcgct actctccctg tcgtatgctg acggacaagt tcatttgctg cttctcttgg
     5341 catatgcaag ataggaatat ccgttgccgc ttgtaactct tcaaaaaagt agtgtgcggt
     5401 gttacaagtc aacacaatga agtttggtcg caataaattt tgtttctcaa tatcatccag
     5461 cagaaacggc attggatttt cttctgatcg atctaaaata taagctgtac gatcaggaac
     5521 tgttgcatga ttgaataaga cataattcaa atattcttga tcttttgtag ctttcgttcg
     5581 gtggttgatc aagcgaacaa aactttctgt cgccatcgtg cccattccgc ctaaaatact
     5641 gaaaaaattc tccatgaact agccttcttt cacatgaaaa tactttttga atcttggaat
     5701 atagttatgg aacatatatt tcatcaacaa ccagcgcttg attgatcggt ctttttcata
     5761 aaatacagta gtaccgtaac gattttcttt gatcatttgc tcagctagtt ttttgtcttc
     5821 attttcccga gcatattcta aaaagatttt tttgggcaca cccatccata atcttgcttt
     5881 atctgattga ttcgttccat aggtcgtttc aacaaagggt ttgttgaaca ctcgatcttc
     5941 tgtgacaaaa cgagccagat tcaagccgtt caatgtcaca aagaaactgc tacgaccttg
     6001 tctcaaattg atttcaaata atttatattc tccatctcta ggatcatact tcatatcgaa
     6061 attggcaaag cctgtatatt cgattttttc caaaaatgcc ttaatggttt gatagatttt
     6121 ttcgttataa tctggcatga tcacaacgta attaccaatc gatgctggtg taggatcttc
     6181 taatagtgga tgaccaaggc acatcatccg aacttgatga tcttcatcta catacgcatt
     6241 tagcacacgc atattactat catcacccgg gataaaatct tgtacaatca tttcactttt
     6301 ataaccggct tcgtatatcc ggcctaaaat taaatcgaac tcttctctag tctctaaaat
     6361 aaaagctttt tttcttcctt caaattggac agaaagatac tccacactgt ttgcaggttt
     6421 caaagcaact gggaattcaa aaggcaattc ctgttccaaa tgtccattta ctaacatttc
     6481 ttctctgacg atcaatgttt taggatacgg aagatcatat tcttcacaaa cttcataaaa
     6541 gcttacttta ttgatcagcc tttcaaataa agaataatca atatatggac agataaaata
     6601 ttctgacagc tcttgtttat gttgggagat caattctgca tatccatcac cacaagcaat
     6661 caaaagatat tttttactct tatcagaata tctttctttt gccaaacgaa gcatcgtctc
     6721 gataaataca gggtctttat caaatcctgg aataacttct acattgacaa ttttactgta
     6781 gcgggtaggg gctaactgat ccgaagcata tgcttggcag attttgccgt atgcttcatt
     6841 aaaagaacgt gccataccat aaacgttcat gtcgcttcct aataatatag ggataaattc
     6901 ttcattttca atactgttca tcattatctc tcacttactc ataatctctt gtttttcttt
     6961 tccaactaca caaggaatat cataccataa agaaaaaagc aatcaaagga aagtcctccg
     7021 attgctcaat tcttttttga acggattgaa cttataggaa gtttttatct ataccaatca
     7081 gtgtagaaag aactaccatg taatgattct agaacactga tattaataaa acaagcaaaa
     7141 attcattttt ttatgttttt ttaactatct cagctatcta tcctctttat accattattt
     7201 ttttcaaata gtgacctgta taactttctt ttacgttaat gatttcttct ggtgttccag
     7261 tcgcaaggat cgttcctcca ccttctccac cttctggtcc taaatcaatg atataatctg
     7321 ctgttttgat tacatctaga ttgtgttcaa tcactaaaac tgtgttacca gcatctacta
     7381 atctttgtaa tacatgcaac aagcgggcga tgtcatcgct atgaagtcct gtcgttggtt
     7441 catctagtat atagaaattc tttccattag agattttgtg caattcactg gcaagtttca
     7501 tccgttgtgc ctcaccaccg gacaatgtcg ttgctggttg ccccatcgtc acataaccta
     7561 agccaacatc aacaatcgtt tgcagtttgc gatgaatctt tggaatgtgc ttgaagaatt
     7621 ctacagcatc ctctactgtc atttccaaaa tatcagcaat gctttttcct ttgtaatgca
     7681 cttctaatgt ttcagaatta tatcgtttac catgacatac ttcacaagga acatagacat
     7741 caggcaaaaa gtgcatttcg atcttaataa ttccatcccc gcgacaagct tcacaacgac
     7801 cgccttttac gttgaaacta aaacgccctt tcttataacc ccgcattttt gcctcgttcg
     7861 tttgagcaaa taacccacgg atatcatcaa atacacttgt ataagtcgct ggattacttc
     7921 tcggcgtccg gccgattggg ctttgatcga tatcgatgat cttttcgata ctttcgtagc
     7981 cggaaattgt cttgaattta cctggcttag cagaattctt atttaatttt tgcgctaacg
     8041 atttctttaa gatactattg accaatgtac tctttccaga accagacaca cctgtgactg
     8101 ctacaaattc tcctaatgga aattcaacac taatattttt cagattgttt tctgaagcac
     8161 cagtgatctt gattgctttc ccattacctt tacggcgttc ttttggtaca gggattgatt
     8221 ttttccctga aagatatttc cctgtcaaag aattttcatc attagctact tcttcaggag
     8281 tgcctgcagc cacgatttgt ccgccttgtt ctccggcccc aggtccgacg tcaatcaaat
     8341 aatctgctgc ccacatcgta tcttcatcat gttcgactac aacgagggtg ttgccaagat
     8401 cacgcatttt ctttaatgag ccaatcagtc gatcgttgtc gcgttgatgc aatccgatcg
     8461 atggttcatc taaaatatac aaaactccag aaagattcga tccaatttgt gtagccaatc
     8521 tgattcgctg cgcttctccc ccagataatg ttccggaagc gcgactcaaa gttagatagt
     8581 ctagtcccac attttccaag aatgaaagtc tgtcatttac ttcttttaaa atcggtttgg
     8641 caattacttt ctcttgttca gataacgtta agtttgagaa gaaatctact gctttttgaa
     8701 tagacaactc atttgtttgc ccgatattcg ttccattgat ttgaacagat agagcttggg
     8761 gattcaatcg ataaccttta catgtctgac aagttaattc tgtcatataa agacgcattt
     8821 gctcacgcgt gaaatcactg ttcgtatcat gataacgacg ctcgatattc ttcaataccc
     8881 cttcgaaagt cgtttctaca tcgcgaacgc caccaaaatc attttcataa tggaaatgga
     8941 aaagatcatc tgtcccattc aagatgatct cctgatgctc ttccggtaat tcttcgaatg
     9001 gtgtatctaa atcgataccg aaactttcac aagcctgcgc taacatttga ggataatact
     9061 gagagctgat tggattccat ggaacgatag caccttgact aagtgtcttc gttctatctg
     9121 gaatcactaa atcttgatcg acttctaatt tgacacctaa accatcacag tctggacaag
     9181 ctccaaaagg cgcattgaat gagaacagtc ttggttctag ttcgccaaca gtaaatccgc
     9241 aataaggaca ggcatagtgt tcgctaaaca acatttcttc ttcaccgatc acgtcaacca
     9301 gagcatagcc gtctgctaat cgcagggccg cctctaaaga atcaaatagg cgagaacgaa
     9361 tcccttcttt cacaatgatc cggtcgatga cgatcgcgat atcatgtttt ttgtttttct
     9421 ccagctcagg cacttcactt aaatcataga tctcaccatc gactcgaacg cggacgtatc
     9481 cctctttttg gattcgttcg aatatttttt tatgttgtcc ttttttcttt accacgatcg
     9541 gtgccaacac ttggatcttt gtgcgctcag gcaaagccat tacttgatct gccatttgat
     9601 ctactgattg actggtaatc tcgatatgat cgttgggaca aatcggatgt cctacacgtg
     9661 caaacaacaa gcgtaaatag tcattgattt ccgtcacagt acctactgtc gaccgaggat
     9721 ttttacttgt tgttttctga tcaatagaaa tcgccggact caatccgtcg atgctatcta
     9781 cgtccggctt atccatttgt cctaaaaact gacgagcata agcagaaagg ctctctacgt
     9841 atctccgctg cccttcagcg tacagcgtat caaatgctag ggaacttttt ccagaaccgg
     9901 acaaacctgt aacgaccacg aatttatctc gtggaatcgt tacatctata tttttcaagt
     9961 tatgggcacg tgcaccatga atcgtaattt tatcgttagc catgttgtcc tcctactttg
    10021 aagcttttaa ttctaaaatc atatctcgca cattcgcggc tgtttcaaaa tccaaagctt
    10081 ttgccgcatc tttcatttct ttttctaatt tcatcaataa cgtgtctttt tcttccttcg
    10141 tcatttcttc gtacgagact tgtaccgctt cttttgtatc atcttccgat tctttcgtaa
    10201 tagaaatcaa atctcggatt tctttaatga tcgtttttgg aacgatccca tgttcttcat
    10261 tgtacttctg ctgaatcgta cgacgtcttg acgtttcatc catcgctaga cgcatagaat
    10321 ctgttacttt gtctgcgtac atgattactt taccttcttc attccgagca gctcgtccaa
    10381 tcgtttggac taaggaacgt tcactacgta aaaagccttc tttatccgca tccaagatag
    10441 caacaaggga aacttcaggt acgtctaatc cttcacgaag caaattgatt ccaactaaca
    10501 catcaaattc acctaatcga agatctcgaa tgatttctgt tcgttccagc gttttgatat
    10561 cactatgcaa atatttgact ttgatcccta attctttgaa ataatctgtg aggtcttctg
    10621 ccattttttt cgtcaatgtc gttacaaaca cccgctggtc tttttcgact cgttcattta
    10681 tttcgcctac tagatcatcg atttgtccca tgattggacg aacttcgatc actgggtcaa
    10741 gcagccctgt cggacggatg atttgttgga taacggtatc tgtttgttca tgttcatatg
    10801 gtcctggcgt cgcagagaca taaatgatct gattgacatg tttttcaaac tcttctaacc
    10861 gaagtggccg attgtctaaa gcagatggca aacggaaacc gtaatctact agcatctgtt
    10921 ttctcgctcg gtcaccatta tacattcctc tgatttgagg catggttacg tgcgattcgt
    10981 ccgccacgat caaaaaatct tcaggaaaga aatccaatag cgtataaggc ggttcacctt
    11041 ctttacgtcc gtccatatgc cgagaataat tttcaattcc cgacgtgtag cccatttcta
    11101 acatcatttc aatatcgtaa ttggttcgct gttctaaacg ttgagcttcc agaagtttgt
    11161 tttcatttcg caagacagtt aaacgctgtt ctaattccgc tttgatattc gctacggcat
    11221 gctccatatg ttcatcattc gtcacaaagt gagtcgccgg gaagattgct acgtgttccg
    11281 tttctcctaa aacttcacct gtcagtgcat tgacttctcg gatacgttcg acctcatctc
    11341 caaaaaactc cacacgtaag gcacgttcat ctcttgaagc cggaaaaatc tccacgacat
    11401 caccgcgcac tcgaaaacga ccgcgctgaa aatctatatc atttctttcg aattggatac
    11461 tgactaagtc acgaatcaac tgattgcgat ctagttctgc tccctgtcga atcgaaacta
    11521 cttgtttttg gtactcaaaa ggcgagccta atccaaaaat acatgaaacg gatgcaatga
    11581 cgatcacatc atttctttct aataatgaac tagtagccga atgtcgtaat ttgtcgatct
    11641 catcatttac acttgaatct ttttcgatat acgtatcgct tgaaggcaca taagcttcag
    11701 gctgataata atcataataa gaaacaaaat attcaacagc attgttaggg aaaaattcct
    11761 tgaattcccc gtacagctga ccagccaatg ttttgttatg ggcaataatc agtgttggtt
    11821 tattcacttt ctcaataaga ttggaaatcg tatatgtttt ccctgttcca gtcgcaccta
    11881 ataagatttg tgcttttttc ccgccgacta cgccatccac taattggtta atcgcttcag
    11941 gctgatcacc agccggttgg tatttcgaga caagctcaaa gtgtcgggag gtatctctct
    12001 caatcattct tcgtcctcct tttcattcat tccttttcat tttacgacca ataaaaagaa
    12061 aaggaaagca aaggcattcc ttactaaaaa attataccac accgaacata ctttcgccac
    12121 ttttttatct tattatacat gttatgtttt ataacataaa atgttagaaa ctcgcatttt
    12181 tttcattttt tcgagcttac agtcttgtct gattcagcac ttttcgccta taataataaa
    12241 atattggaaa tttaggaggt tgattatgaa aagaaagcat ttatttcact catttgtttt
    12301 cttaatgaca ggacttgtga catttgcgtt tggtcatgtt gcacaagctg acaacactac
    12361 aacggcagag tcaagcacaa gcgagacaat gcaaaaaata gagcccaaaa aagacgtata
    12421 cgtaattgcc agtgactctg cttttgctcc atttgaattt caaaacgcaa agggcgagta
    12481 cgaaggcatc gatgtaaaat tgatggaaaa catcgccaaa ctgcaaggat ttaacatccg
    12541 aatggactac cgtggtttct ctgctgcttt gcaagctttg gaatcaggac aagcagacgg
    12601 aatgattgcc ggaatgactg taacagatga acgaaagaaa agctatgatt tttctgattc
    12661 ttactttgat agcgggatcc aattagctgt caaaaaagga gatacttcga tcaagtctta
    12721 tgaagatttg aaaggaaaaa cagtcggagc aaaagttggt accgaaagtg ctgaattttt
    12781 aagcgaccat gaatctgaat atggctattc tatcaaatat tttgatgctg ctgatcaatt
    12841 gtacgacggg ttaaaagttg gcgcaatcga tgcaatgatg gatgattatc cggtaatcgg
    12901 ttatgcgatc aaacaaggac aagaattgga aacaccgatc aaaaaagaat ctggcggaaa
    12961 atatgcattt gccgttaaaa aaggacaaaa tcctgagctt cttaatatgt tcaatgaagg
    13021 attgaaagaa ttaaaaagaa caggcgaata cgataagatt attaaagaat atgtagcgga
    13081 tggttcagaa acttccagtg agtcttccac tgctgatgaa tcaacatttc ttggacttat
    13141 ccaaaacaac tatcaagcat tgttgaaagg actttggaga acaattctcc taactttgat
    13201 ctcctttgct ttggctatag taatcggtgt gatttttgga ttattcagtg tagcaccaat
    13261 acgcgctctt cgcacaattg ctagtattta tgtcgatatc atccgaggaa ttccaatgat
    13321 ggttcttgcg ttctttattt tctttgggtt gcctggtatc gttggattta cgattcctga
    13381 cttccttgct ggtgttatca ccttgactct taacgcaagt gcttatatcg cagagattgt
    13441 tcgaggcggg atcaacgccg ttcctgtcgg acaaatggaa gcttccagaa gtttaggtct
    13501 ttcttataat cggacaatgc aaaaaatcat tttgccgcaa gcgatacgca tcatgatccc
    13561 atcattcgtc aatcagttcg ttatctcatt aaaagacaca acgatcattt ccgcaatcgg
    13621 tgtagtcgaa ttgctgcaaa ctggtaagat cattgttgca cgtacaacac aaagtacgta
    13681 tgtttacttg atcattgcga tcatgtactt gattttgatc acagcattga caaaattagc
    13741 aaaagtttta gaaaagaagg tgaagtaaga tggcagaaaa aatcttagtt gagcatttag
    13801 tcaaaaaata cggcgataac acagtattaa acgacatcaa tgtatccatc aacgaaggag
    13861 atgtcgtctg tatcattggc ccttcaggtt caggaaaaag tacattcttg cgttgcttga
    13921 atcgtttaga agaaccttct gacggtgaaa ttattattga cggcgcccac ttgatggata
    13981 aaagcacaga tatcaacttg gtgagacaac atatcggaat ggtattccaa cactttaacc
    14041 tattccctca tctttctatt ttggaaaaca tcattttagc accgatggac ctgaagaaag
    14101 aatctagaga agaagcagag aaaaaagcaa tcaaattact gcagacagtt ggtctagaag
    14161 ataaaaaaga tatgtatcct gaaatgcttt ctggtggaca aaaacagcgt gtggctattg
    14221 ctcgtgctct agcgatgaac cctgacatca tgctttttga tgagcctact tctgcactcg
    14281 atcctgaaat ggtcggtgat gtcttgaacg tcatgaaaaa gttagcagaa caaggtatga
    14341 cgatggttat cgttacgcac gaaatggggt tcgctaaaga agtggccaat cgcgtcatgt
    14401 tcattgacga cggaaacttt ttagaagatg gaacgcctca acaaatcttt gaaaatccgc
    14461 aaaatgaacg gacaaaagat ttcttgaata aagtattaaa tatttaataa aaaccaaaaa
    14521 tgcacggtcg ccatgaaggg accgtgtatt tttatcttct tattttctca cctaatcgta
    14581 tcagccgcat cgcttgtttt cctgcacaaa caagcaatcg ctctacctgt tccatcgcat
    14641 aagaaagtac catcggttca ttgatgatat caagtacata gtcgatacca ccctcactta
    14701 attgagcttg atttttcttc tccatattat gtagttgtgg gacaactttc tgacaatcag
    14761 caattgtgtc aagaacggat caatccttta ttggagttgc tcgaaagggt cttctcttat
    14821 tacggacagg ctttgctcac tgtacataga caacaaatca ttatcctagt caatcggatt
    14881 tccagagcct cattgctatc attgcttgat aaaatccaga caaagtttaa taaaatgtat
    14941 cagttacagc taaactttgg aattgggtcg ctttgttata cagagcaaga gacaccgcaa
    15001 tctttccttc atgccaaaca agtttgcgaa tggatagctt tccatcaatc tgtgaacgag
    15061 atccgctttt ttgaagattt agacctcggg atcgttttac cagcaatccc gtcagatcaa
    15121 cgaacattat atgtaaaacg tattttgaaa agcctgacag aagaagaagt acatttgttc
    15181 aagaagactt tggcatgctt cagtaaaaat aacggaagca tcaaaaattg ctcagaagaa
    15241 ttatttattc ataaaaacac cttgcaatat cgattgaaca agttccattc tctcactggt
    15301 tataccccta gaaactatga cgattatcat attctaaagc tggctttttt gttggtacaa
    15361 acatgacaac tacaaaaaaa ccaggcgaac atatgattgt tcgtctggtt tttaattatt
    15421 aatgacttag cgtttttact agaaaaacta cttccgcttt ttcccgccaa aagcatcttt
    15481 aaacttatcg aagaaaccgt cttcttgctg ttcttcaact ttgatgccgc cagcttcagc
    15541 gaatgcacgt aatgcttctc tttgttgttc atttagattt ttcggtgtga tcagttttac
    15601 tttgacatgt tggtcgcctg ttgcaccacc acgtaacttc ggagcgcctt ttccacgtaa
    15661 acggaagttc gtacctgtct gtgttccagc agggatcttc aatttcacat ctccatggac
    15721 agttggtact tggacttcat cccccaaagc tgcttgtaca aaacttaaag gcaattcata
    15781 gtaaatttca gaaccttcac gatcaaagat atcactttct tctacacgga agaccacata
    15841 caaatcacca aatggtccgc cgttttctcc agcttctcct tgaccagcta aacgcatttg
    15901 ttgtccttct tctacaccag ctggtacatt cactttaact gaatgtgctt tcttctcatg
    15961 tcctgagcca tgacaggttg gacaaggttc tttgatttct ttccctgtcc catgacaaac
    16021 atcacaagtt tgacggctca tcactcgtcc aagtggtgtt tggcgttcta cattaatcgt
    16081 acctgcacca tgacatttat gacaagtagt tggttgtgtg cctggttttg caccgttccc
    16141 accacaagta tgacagattt cttcccgatt atacttgatt tctttttcta caccaaaaat
    16201 cgcttcttca aaagacaagt cgaccgtata ttgcaaatca gcaccttgac gaggcgcatt
    16261 tggatcgact gaccgaccgc ctcctccaaa gaaagagtcg aagatatctt caaaaccacc
    16321 gaagccgcca ccagagaagc cgccgaatcc accaaatccg ccaccagctc caccagcacc
    16381 gtaatttgga tctgtgcctg catgcccgta ttggtcatat gcagcgcgtt tttgcgggtc
    16441 acttaaaatc tcatatgctt ctgatacttc tttgaatttt tcttccgcat caggttcttt
    16501 attgatgtca ggatggtatt ttttcgaaag cttacgataa gctttcttta tttcatcttc
    16561 tgatgctcct tttgagagcc ctaagacttc ataataatca cgttttgttg ccataggctt
    16621 tcctcctatt ggttctcatc attttcacgt cgcttgttat catatcatat tttgtttgaa
    16681 acaaaaaccg atacttcaaa ctatttttca aaagaaaaag ccaaagtagc aactttggcc
    16741 ttctctcgaa attcagtgaa ctgattattt atcgtcgcca tcgacttctt caaaatcagc
    16801 atcaacgaca tcatctgctc cgccttgagc tgcttctggg ttttcttgag cttgttgttg
    16861 tgccgcttgt tcgtatagtt taacagtcaa gttttgaacg atttcgttca atgcatcgcg
    16921 tttagttttc atttcttcga tgttgtttgc ttcaacagcg gctttcaatt catcgcgagc
    16981 tgtttctgct tttttcactt cttcttcgtc cactttgcct tctaattctt tcaatgtttt
    17041 gtccacagag aatagcaatg catctacatc attacgtagg tcaacttcct ctttacgagc
    17101 tttatctgct tctgcatttg cttctgcatc ttttaccata cgttcgattt cttcgtctgt
    17161 tagacctgaa gaagatttga tcgtgatttt ttgttctttt tgtgtaccta gatctttcgc
    17221 agatacattt acaatcccgt ttttatcaat atcgaatgtt acttcgattt gtggaatacc
    17281 acgaggagct gctggaatat ctgtcaattg gaatcttcct agagttttgt tgtcagcagc
    17341 cattgggcgt tcaccttgta atacatggat gtctactgct ggttgattat ctgccgcagt
    17401 tgagaatact tgtgatttac ttgttggaat cgttgtattg cggtcaatca atttagtgaa
    17461 cactccaccc attgtttcga tacctaatga caatggtgta acgtcaagta aaacaacatc
    17521 tttcacatca ccagtgatca cgccaccttg gatagcagca cccatagcaa ctacttcatc
    17581 agggttaact gatttgtttg gttcttttcc tgtttcttta cgtactgctt ctacaactgc
    17641 agggatacga gtagatccac cgactaaaat aacttcgtcg atttctgatt gtgataaacc
    17701 tgcatctttt agcgcttgac gtactggtac ttttgtacgt tctactaagt cagctgttaa
    17761 ttcatcaaat tttgcacgtg tcaatgtcat ttccaaatgc aatggtccag attctcctgc
    17821 tgtgataaat ggcaagctga tttgtgtgct tgatacgcca gataaatctt ttttcgcttt
    17881 ttcagcagcg tctttcaagc gttgtaaagc cattttgtct tgagacaagt caatgctgtt
    17941 ttcttttttg aattctgcta ccatgtagtc gatgattttg ttatcaaagt cgtccccacc
    18001 tagatggttg tcgccagctg ttgacaatac atcaaagaca ccgtctccta gttcaaggat
    18061 agatacgtca aaagtaccgc caccaaggtc gaatactaag attttttcgt cgcgatctgt
    18121 tttatctaaa ccataggcaa gagcagctgc agttggttca ttgacgatac gttctacttc
    18181 tagaccagcg atcttaccag catcttttgt tgcttgacgt tgtgcatcat taaagtaagc
    18241 tggcacagtg ataactgctt tttcgacttt ttcacctaaa tagtcttctg cgaatccttt
    18301 gatatattga aggatcattg ctgagatttc ttgtggtgta tatgatttac cttctacatc
    18361 tactttgtaa cctgcttcac cgatatggcg tttgattgat gaaatcgtat ttgggtttgt
    18421 cacagcttga cgttttgcaa cttcccctac ttggatttct ccatttttga atgatacaac
    18481 agaaggtgtt gtacggtttc cttctgggtt agcgatgatt tttgcttcgt ttccttccaa
    18541 tactgctact gcagagttag tcgtacctaa gtcaataccg ataattttac tcataattaa
    18601 cgtctccttc tttaattgaa agtgtaagtt ttatatccgc acaattattg tgcaacaatg
    18661 accatggttg gtcttaacac gcgatcatgt aatttgtagc cttcttgtag tacttctaca
    18721 atcgtatcgg ctggtgtatc ttcagtcgct ggaacagttt gtactgcttg atgaagatta
    18781 ggatcaaagg cttctccttt agcagggatt ttttcaattc cttcttcttc cagtgcgtta
    18841 cgcaaacttt ccaagaccat ttccacgcct tttttcaaac ctgccccttg atcatctgac
    18901 acttctgtgg ctaatgcacg ttccaggttg tcaattgatg gaagtaattt tttggctaaa
    18961 tcttgtgaac ggtatttttg taattgctca cgctcatttt ttcctcgatt tgccatattt
    19021 gcgatttcag cacgagcccg caagtattta tcttccattt cttctaactc agcttttaat
    19081 ttcgctgttt cgaattcttc cgcttcagct tctgaaatac ccgaatcttc tgcagcttcg
    19141 acatcgattt ccggttctgg ctgagcatct gtcatttctt tatctagttc ttcttgattt
    19201 tctttcacga tacagtcttc ctttcattat tcagtcagct gtttgattgc tcttactaag
    19261 aaagagagaa cgatcagctg caaatagctt cgttattcac attacgagtt tgacaagtca
    19321 agcgaacggt agtaatcagc aagtttagct gctagttctc ttcgataaac atcgactaga
    19381 ccgaacatcc gagaataagg catactagta ggccctaaca aggctattgt acctcttcca
    19441 tgaccatcta tctcgtaact tgcttgaatc atactcatat tctgcaataa atcattgcct
    19501 aattcagcac caatcttgat atgaatattg ctatccatcg gagtgatcat ttgtgtaagt
    19561 ccgtctgagt ctttcataaa agaaaacatc gatttgaact gatcaagatc ttgacggggc
    19621 tcataatcca agagattcat ttgtccactg acataaacct tatcttcaaa aacatgacca
    19681 agaacggtgt caaataactg agacatccct tctgtcgttt ggaaatattt atgaagaatc
    19741 atcggaatct ccgtacgaag tttatggtac accgtcatca gaggatctcc gactagacgg
    19801 tcgtttacaa tctgtaccat tttttctagt tcctgacttc ccatatattc gggaagagtg
    19861 aacacttggc tttctacatt ccccatatct gttacgacga tagcaataat ctgtcgatta
    19921 ttcaagggta cgatacgaaa gccagtcagt cggcggtctt ttatttcagg tcccaatgaa
    19981 aaagtcgtat agcttgtcag ctcagataat atctcagcag attgcttaat aatctcattg
    20041 atttcatgaa attctctgcc tagagactga cgaatcagca gcaaatcatc ttctgctacg
    20101 cgagcaggtt ttaacaaatg atccacgtaa taacgatagc ctaaagcaga tggaatccgc
    20161 ccagaagaag agtgagtctt caacagcaat ccttcatctt ctaatgcttt catatcattg
    20221 cgaatcgttg ctgagctggc cttgactccc tcagacatca atgttttgga accaacgggc
    20281 acgccactac tcgtatagtg ttggatgatc agacgtaaaa tgtcgttttg tcgctttgtt
    20341 aacatcttcc tcacctctca ttagcactca tatattctaa gtgctaatcc acttataaat
    20401 atagcaagaa gcccttctcc tgtcaagaga aagactccat tttttagcac tctaaacaca
    20461 agagtgctaa ttctaaataa ttgatgtaaa tccagttata tcaacgtttc aatagtcctt
    20521 tattgtattt ttgaactttt tttatttaaa ttcagataaa aaacaaatat tctttaaact
    20581 atttttcaaa caaaaagcgc tcaaacacat tgttccccaa aaaggtccct cttggtgtca
    20641 aataaacacg gtcttcttca ttaacaagta acccttcttg ctctaattcc cttagcacat
    20701 caccgtacac cgattcaatt gttttttgat agcgatcata gaaatgtttt ttagtaatcc
    20761 ctgttttttt tcttagtcct aagaacattt cttcttctat ttgatttttt cgtgtcaatt
    20821 tttcttcttc caataccggg cgttcatttg ctcgtaatgg gcgtaaatag tgttggattg
    20881 gtcctttgtt gcggtaacga atatctccaa gataaccgct agcaccagct ccaaacccaa
    20941 aatagtggtc attgttccag tacatgaggt tatgttgaga ttcatgtccg gagagcccaa
    21001 agttacttat ttcatattgg tgacgtccat gcttttccat tgcttcaatc gtctcttcaa
    21061 acattctggt ttccgtttcc tgatcaggca gttcgagtct tccttgtcgt acccagttca
    21121 tgaacattgt tttgttttcg aggatcaatg agtacaaaga atagtgaggc agatccaaag
    21181 ccaaagcttt ttccaaagta tccagatatc cttctagtgt ttgtcccggt aatgcataaa
    21241 tcaaatcgat gcttacattg gtaaaatttt ctgcttctaa aaattgcata gtttgataca
    21301 catcttctgc ggtgtgttta cgtccaattt ttttcaataa gcgattatca aatgtctgca
    21361 ctcccatgga taaacgattg acgccatagt ttttcattac ttgaagtttt tcacgggtca
    21421 aatcacctgg attcgcttct acagtaaact cattaccatt ttgaaaaggc aggatctctc
    21481 tagcacctga taacagtcga tcaagttgtt tagcagataa agaagttggt gtcccgcccc
    21541 ctacgtaaag tgtttctaaa ttcgctgtcg gatgactttc tagcattatt ttcatttcat
    21601 tcagcagcat ttccacatac tcatctactg gctgcccttc taaaaaaact ttattaaaat
    21661 cacagtaata gcagatatgc tcgcaaaatg ggatatgaat gtacgcagaa accttgtttt
    21721 ctgcgggatt tattgtttct atcattgaaa ttctttctcc ttacgtcgaa aagtcgtgcc
    21781 tgttttcgac cttcttaaaa tcgcgttcaa gcaccaactc attgaaataa gccgcatatt
    21841 tagaatcaag gaaacctagc tctaaaaatg cttgcttatt gtcatcggag ctacacggtg
    21901 cttttacagc ctctcgataa acggtaatcg caaagctgac cttcggcatt aagtcaaaat
    21961 ctgcaaaaat atgaaaagct attttcacag attcctcctt aagtgctcag accacaacgc
    22021 tttgcttaca acctctcata tacgcatact aaacgtattt ggcgaacaaa tccactaaaa
    22081 aatccaagaa tcgacttctt ggatgatttt ttctttcatt aaactggtta cctagttata
    22141 tatctctagg gttgttttca tgtctgagtg cccgatatga tttatttttc gtttctataa
    22201 ttactttttg ccttccagtt tcttttaata ttgtactcaa gcaagtggga attggttctt
    22261 ttgtcatcca tgtatgcaaa atgttataat gctggtaaac aaaaatgatt acctgtcatt
    22321 tttatttagt tcgtatttaa ttaagttgac tatctgtata ctctgtctaa aacttaagaa
    22381 ttgttgtttt cgctgtcgct acccaatttt aatgaatgtc tgtaggttaa aactgttgtc
    22441 cttgttatag atagtaaagg agagcttgaa aatgaaaact gcgattacgt ttctattaga
    22501 ggaatatgcc gattgggaag gtgcttatat tactagcaca ctaaaccaaa atgagaaatg
    22561 gcaaaacaaa actgcttcta tccaatcaga ggtcacttcc attggaggat taaaaacaaa
    22621 aatcgattac ttaatcgaac aaatacctga aaaagttgac ctttttatta tgatcggtgg
    22681 caattcatgg gacataaaaa atcatcattt atatcaaata atcgataaat tgttaaaaac
    22741 agacgtacca gtagctgcta tttgtggggc agttgattat ttagctcaga gcggattatt
    22801 gcaaggttac aaacacaccg gaaatgcgca atttccttgg ttagaatcgg aaaattatca
    22861 gaataaagaa gattttatag aacaacaagc tgtgaaagat cgtaatttga ttactgcgaa
    22921 tggaacagct cctatagaat tcacagactt gattcttcaa gcagtccatt ttgaaacaga
    22981 agaaataaga gaaaaaacga ttcgtctata caaacttgga ttctatgatt actataaaaa
    23041 gtacggagat cctttctcct aatcattctc ttcaattaaa tactccttat caatagtgtg
    23101 acaagtaatt ttactgatga ttatttgtca cactttttct gacatttatc aaagaagata
    23161 cactctattt atattcatag acaaggtttt acggattcaa aaattcagga ttatgctata
    23221 ctggtattaa aaaaataatg aggtatttta tatgaaaaag ttaggtttta ttttattgtt
    23281 ggtctcttta ttccttgtag gatgtacgtc tcctagtaaa gaaaatagtg atccatctgc
    23341 gaccacagac aaaagttcaa tcagacaaac cagttcagct gaaaaatcaa gtactcagaa
    23401 atcagcttct actgaaacga atacatcttc aacaagcagt aaagtctcag agcccaatac
    23461 ttcttctaca tctagtagta cagttgagac aaatatttct tcttcaacaa tagacacaac
    23521 ctcctcacag tcttctcagt caatagatcc gttagcagga tattctgatc tacaaattga
    23581 gtacgcacgt gtttggctag ctattagagg taatactttt agagatacat cgggtgattt
    23641 cgaattacat gttattcatt atcctgcagg aacacctatc gccccttatg atgcagggag
    23701 tgcagtctat ccgaaagatg tggttatgct cactgggaaa tacagttacc agggagtttt
    23761 agtgtattca agtaaccatg acgggacaat cactagttat ccagttcctt cacattggca
    23821 aatgcctgca gatcaaacat cttatccagc gttcattaga gaaacaactc aagaaatcat
    23881 tgataatgca tctgtagtag ctattcctac aggaaatccc aatgacgtga aacagttaat
    23941 cgatgtgaca gttatagata attagacttt ctaatatgtt atacaaaaag aagctgacag
    24001 cattttgtca gcttctttca ctttcttttc tatttagatt tattggtctt tgatagactc
    24061 caaggcagga aaaaattttt taaataaacg tttgcaaaac agtcgctttt ctatcttcta
    24121 ccgctacttc tgcataggca ccattaatca atgtcagttg tggcggtaca tgaccacaat
    24181 caatatcata caagatcggt agttttaatt cctttgacag atcttcgtat acatccttca
    24241 ttttataatc taacaaatcc tctgccgcac ttctaccgaa catgatcccc gagctgccat
    24301 caaaccagcc tgccagtttc atctgaacaa gtgatcttct taaatcaact gacgatagct
    24361 cacagttttc tagaaaccac aagatcggct ttccatcatt atactttctt ttaaaagatt
    24421 ggacatctcc ataaggagta ccgattaaat gcctgatcgt atcaatacaa ccacctaata
    24481 atcgtccctt catccttact gcatctccgc tgactgattg ccaatttgtt ggttcagtga
    24541 gatggaaaac agaagatgat gaatgctcat ggggccacac tttttgatac ttttcagaag
    24601 attgctgtag aatcgattct ccttctcttg ttttcagtac actttcccac attgctgtcg
    24661 tttcatccaa gtactctcct cttaaatcaa taaggttagt tccatgtgca gtggctatcc
    24721 ctgtttttaa tgtgacagca agtagcaacg tactaatatc tgaataacct actatccatt
    24781 ttggttgtag agcatcaaaa tcgacaaaat cgagaatctc aatcaataat tctcctcccc
    24841 aagtcggaaa gatcatttgg atattttcat cttgcatcat ttgattgaat tcatctgctc
    24901 gttttcttgc tggacctgat ttttccttat cttgcgtcca aagcgtcttt ccagtaacta
    24961 cttggaaaga tttttcttcc attctcttta aagctgattc gagtaacgaa tggtgttcct
    25021 ctgatacgcc tgaggatggt gcagtaaccc caatcgttgc atttctttct aataatggat
    25081 aacttatcat gttgcttcac ctccaagtat gcactatttt acttggttaa ttgttttttt
    25141 cgtttgccgg catcaaaaat atcggcatct caagtagctg gatgattttg tttaagtcaa
    25201 ccgtttggat gaccatcgtt tgatctggtc tacctggtgc aaaaacaagt ttttcatttg
    25261 ataagagctc ttcatcaata ataatcggaa tctgtttttc ataaccaaac ggagaaacag
    25321 cgccctgctt ttgtcccgtt tgttcttcca tgcgttctga tgaaacaata gaaagccttt
    25381 taccgacgat cttccctaat gctttgaaat ccgtttgttt cgtagtaaaa gtcagatata
    25441 cgtagttatt atctttttta tcttttaaga ataagctttt ggtctctgta cctgtaaatc
    25501 cttgttcttt ctttattacc aaagcatctt catttgtata aatggctcga tgatgataca
    25561 actcaaaata tatattttcg tgtaagaatt tctttgccaa gtcaatcatt tttatgtctc
    25621 ttttctattt tattatttga gacatcttat catataaata tagcggttac aacattactt
    25681 gtgttttgtt cgttttttat acataactat tgtttttctg gtaaaataaa tcaaaccact
    25741 taatggaggc gttttagaat ggaaaaattt cagccgatca tcaatcaaat caaagaaaaa
    25801 gaacatcaac aaaagatcaa agaccttttt gctaagataa cagaaacgta tccccagcta
    25861 gacacaactg taaaatggaa ccaacctatg tttactgacc atggtacctt tattattgca
    25921 tttagttttg ctaaaaatca tttttctatc gcaccggaaa aagcagcgat tcgtgccctt
    25981 gaaaaaaata tccaagaagc aggctacgta tatacggata acgtcatcaa agttccttgg
    26041 aaaagtgtaa tcaactggga attgattgaa caattgattt catttaatat tgaagataaa
    26101 aaaggtcatg ataaattttg gcgataaagc tttatacgaa taaacctttt actggcgagg
    26161 aaactattca tcaccttata caggttttgt tcaatcctat cctttttatc atccctggtt
    26221 aaacacttaa ttgacaaagt atctgctatt tttataagga gtctttcttg tgctgaaaaa
    26281 aatcactgat tgaatggctt tgaattttct acatagtaaa attatttttt cagcaatttt
    26341 cctgcaaaac ttttgaataa ggaaaaaata cgagaaaata tcagtgatct ttcataatac
    26401 tgcccattat tttcaggtat atttaaatat aatatcactt taggagagaa cgaatttctt
    26461 tagagaaata aaagttagaa ttttctgact tgatttttac aatgacctta attaaaatcg
    26521 ttctttctac atttcgtgat taagcaaata tcaactaagt cagtagaacc attcacatag
    26581 gagggtgaac attgaaagat ttttactcag taaatgaatt agccgaacaa ttaggtgtta
    26641 ctacacgttc tatcagaaat tatcttcatg aaggaaaact aaaaggaaca aaggttggcg
    26701 ggcaatggaa attttctgaa cgtaatcttt ttgagttcct atacggagat caagcagagg
    26761 aagcagcaaa agaaatgcag cgattcatgc taaatgcacc aatcacgatg cgtttcaacc
    26821 ttcaatatcg tgattttaca gcaatcaatc aattccgaga acaattagtc cagtaccata
    26881 acgatgttta cgcaaataaa aaagaccgtc ttttacaata cgatctttat aaagacaatc
    26941 atgcggagat actaatcggt ggtaacttca attatgtcgt aaactttagt caatggatca
    27001 atgaaaaatt attgatccag acagatattt cccttgtatc ttaaacaatt aaaaagcgct
    27061 agaaattcca gagatgttgg aaattctagc gctatttttt tataagtact tactgtttgg
    27121 ttcaaacaaa tattttttta ataaatgaaa ttttaccatc tttatcctca aataaagacc
    27181 gtgaccatgc ttcctactac gatcagcaat aatccgataa tcgttaattt tttttctttt
    27241 ggtgtcttat tttcatgcag gatgtacata ccgcctaatg tagagataac tacgtttagc
    27301 tgtgtaatga tatatgcagt agcaacccca ttttcatttg ctgaaaaaat ataagccaat
    27361 gctgacacac taaagatggc tccaacgatc gttgctttcc aactcttgtc ttcagaaaat
    27421 gcggttggtt tacggaaaat cacataaatc actgccccta aaaagacacc gatcatttgc
    27481 ggaaagaaaa tggaaagtcc gcttgcatca actagtttag gcaatgcact gtatacccaa
    27541 taaccaatac ttgttgatgc taatagacta attccttttg tgataccgcc tttttttcca
    27601 ggatcatcac tgattgccgt taaagcagaa ccaaatacaa gaagaatgat tgctgctgct
    27661 ccaatgattt tccccatact tccagaccat tctccaaaag cgaagacacc aatcaacgag
    27721 gttccgacta actgtaatgc agtcgaaatt ggcatcgttt tagaaacacc aatcaagttg
    27781 aaagatgtat actgtcctgt ttgtccaata acccaaaatg caccagaaag taaactaatc
    27841 aaaaatgtag tcactgaaat accagctgga gaagcgaata attgaacgag taagccgaca
    27901 agcaccgctc caataccagt tcctaatatc tcatttgttg gtctgccacc aatctttttc
    27961 aaaatcagtg gctggatccc ccagccaact gccgggatca atgcaatcaa taaacccatt
    28021 tttattcacc cttatccttt tccaccttgg aaagaaggat ataaagtcat cccaccatct
    28081 gcaaatagtg taatacctgt aacataactg gattcgtctg aagccaacca tgcagctgtc
    28141 gccgctattt cttttggttc acctacgcgt cctagtggga tcatactttc caagagttct
    28201 ttttgttcag gatcatcaaa ttttgccgca ttaataggtg tacggatagc accagggcct
    28261 atactattga ttctgattcc cttcggtgca tactccattg ctaccgtttc ggtaaataat
    28321 tttaccccgc cttttgaagc agcgtaatga gcaaacgttg gccatgggat ctgttcatga
    28381 acagaagaca tattgatgat atttcctttt ttattattct ctgtaaagta atttaaagcc
    28441 gctttcgttc cgataaaaac gccagtcaaa ttgactttta acactctttc ccaatcttca
    28501 aggctaagtt catgggttgg atgctgattt tccatcccag cattattaac ccaaacgtcc
    28561 agtgtcccaa aattttctaa agccgtatca agtaaacgct gtattccttc ttcagaactg
    28621 acatctgctt gtacagagac tgcttttccg cctgcctctt cgattgcttt gactgctttt
    28681 tctgctcctt ctttatccga gttatagttg acaactacat tcattttttc tttcccgaat
    28741 cgttgagcaa tcgctgttcc aatccccttt gagccaccag ttactactgc gactttgttt
    28801 tctaattcct tgtacatagt acccactcct ttttcatacc taaacctgtt attacggata
    28861 acttttattt ccgccttaaa gtgagtgtac accaagatat atagtctaaa gcaaatgata
    28921 cgctctagca atcgttttca tgaattgcta gtgtcctttt atttctataa agaaagaggt
    28981 tgtgacagaa gtggtcagct tcaagaaatt tcggcttatt tccgaagaag ctacttctga
    29041 aaaaccattt atttggaaaa aaaggctgtg acaagacttt tgtcacagcc cctttcctgg
    29101 gcttatttat cacccatttt attgtaattg ctagttatac ataaacaaga gattttatgc
    29161 ataatgtttt attgctgaga aaaatattca gctgttgctt gttcatcctc cttgagttgt
    29221 tcaatcaatt cctctacact agaaaatttc acttgatctc ttaaaaattg attccagcgt
    29281 accgcgacat gttcaccata gatatcttga ttaaagtcca aaatatacaa ctctacagtc
    29341 agctcccgtc cttcaccgaa tgtatcatta tgtccaattg aacccatggc tggataccat
    29401 gtttccccaa tttttatttc cgacacataa acaccttctt taggcaaatg aaccgtgctc
    29461 tttactttga catttgctgt agggaatcct agtagtctgc ctcgtgcatc gccatggaca
    29521 actacccctt ctacctcata tgtgcaacct aatagttttg ctgcttcttt tacttgtccg
    29581 ctgtctagta atccacgaat acgggtagag ctgatttttt caccttgttc ttcttcttta
    29641 gaaactgtga cgattttgaa tcttcctttg gcatattcag gaaaatgagc gacatctgca
    29701 atgtcttttg gtccatacgt ataatcaaaa ccggaaacag catattttgc atgtaaccct
    29761 acgatgtatt gatcaacaaa ttcctgcggt gctaaatgag caaaagaaga agtaaaatca
    29821 acttcatata aataatctat acccaaagcg gccatttttt cttctttttg cttaaggcta
    29881 gtcaaatatt tcacgctgct aggatcgatc tttttaaaga caatagatgg atgctgattg
    29941 aaagtcatca atgctaattt caatccttct ttttgtgcaa tttccatacc tgtctttatg
    30001 actttctggt gtccgatatg cacgccgtcg aaaaatccca gcactaaaac gacatcgtct
    30061 tctggaattt gttctgattg gtaaggatgt ctgatcttga tgatattcat tcgttattcc
    30121 tcatttctca atactttact aggttttaat ttgttttttt ctttcgggtt tggctgataa
    30181 atactgacaa cttttccttt ataaaacaaa gcaatttctt ctgacggcat ttctgggagc
    30241 gcaaatacag tataatcaag tctcataccg ttttttactt tctgccatac ttcatcagta
    30301 atatctactc gtttgaaccg ttccactcct ttttctattg gcagcaaaca atcatcgatt
    30361 gtcccatttt tcacagcttg agctacttct tctaatgtca gagcatcctt ctgggacata
    30421 ccggcacttg ctgttcgggt cagatcagac atgtgcgcag ggtaaccaag tttcttgcct
    30481 gtatctacag ctaatgtgcg gacataggtt cctttcccgc attctacttc gaagcgccaa
    30541 gaaatggttc ctgtcacttc gtctcttgtc aattcacttg tccggataaa acggtaaatc
    30601 tgagcctttc taacaggacg ctccactgtt tcattattgc gggcatattc atataaccgc
    30661 ttaccgttga cttttaccgc tgagtacatt ggtggtatct gggtgatctc accaataaat
    30721 gaagccatca cttcatctat ttttttttca gataaagaca tatctactgg ttttgtttct
    30781 acccgttctc cggatcgatc ttccgtcgtt gtactgaaac ctagagttat ctcgcctaca
    30841 tagattttcc cagaatctgt caaatattcg ataacttttg tggcttttcc aatacaaatg
    30901 ggcaacacac cgtctacgtc tgggtcaagc gtacccccat gtccgatttt tttcgtatgt
    30961 aagatttttc ttaatttgaa tacgcaatca tgacttgtca ttccgcgttc tttccataaa
    31021 ggtaataacc cgtccatgtc ttttctccat ttcatttttg atttaactga attattataa
    31081 cacattgcat caattactga cgaatttgac atcataatga acggttttta tttttcggca
    31141 ataaaaacgt aacaaaattc atgctcacgt aaatagaaat catgttatgc ttttttaaag
    31201 gagggaatag aatggaagat tttttctttg caccatttga ttggatttat gggtggctta
    31261 caggtaaata gtttggtttt cacttcccaa aaacgaaaaa aaagactggc cattcttcca
    31321 gtcttttttc cgtttttttt atttattttt tcagtttctt aatcaatgta aaaaattcta
    31381 ttttgcggga tcgattttat cattttattg gtcaagattt ttccatttcc aatccaatac
    31441 ttctggcaga tcaataccat tttcacgaat atagttttga tgttcttgta atttctgatc
    31501 catttttttc gcaaaatctt ctgcttgact tcctttgatt tttaaagctg cgtcttttgc
    31561 caaatgataa cgatcaagtt catttactac acgcatgtca aatggtgtcg tgatatctcc
    31621 attttctttg tagccatgaa tcattaaatg atggttatga cgatcaaaga aaatcgtgcg
    31681 gaccaatgtt tcataaccgt ggaaagcaaa taagatcggt ttgtcttttg taaaatattg
    31741 atcaaattct tcatcgctca acccacgcgg atcttgactt ggatgacgta atttcaataa
    31801 atctacgaca ttgatgaacc gaatttttag ttttgggaat tcttccagca gcaaagttac
    31861 agctgctaat gcttctaagt tagattccgt tcctgcagca gcaatgacca attcaggttc
    31921 ctcctctttt gctgtcgaag cccaatcaat gattttcaag ccttcattca ctaagacggc
    31981 tgcttcttct gctgagtaaa attgagcacg cggatgtttg gaagagatga tcaaattgat
    32041 tttttctgac gagcggaatg ctttatccat gactgcgagc aatgtatttg catccgcagg
    32101 cagatattca cggatgaact ccgccttctt ttctgccaaa tgtgtcaata ttcctggatc
    32161 ttggtggcta tatccattgt gatcttgctg gaagactgtt gagcttgcaa tgagatttag
    32221 cgaaggataa tcatttcgcc aagaaaggtc atgtgatttc cgcatccatt tgaagtgctg
    32281 agtcaacata gagtcaacca ctcgcaagaa tgattcgtaa cttgcaaaaa accatgccgt
    32341 ccagtcagaa cgtacccttc taagaaccct tcatcttggt gttccgataa ttgactgtcg
    32401 atgacgcgac cagatggcga aagccattca tcattttctg gttcgatttt ttccatccat
    32461 tggcgatttg tcgttttgaa aacttgattc aatcggtttg attttgtttc atctggtcca
    32521 aatatacgga aattttctgg atttttctca atgatctcgc gaacgaattt accaagttcg
    32581 gtcatatctt ctgctttaac ggagcctggt ttttcaaatt gcaaagtata ttttttccaa
    32641 tctggcataa taagcggtct tggatcgatt ccaccattgg tgataggatt ttttgccatt
    32701 ctttggtcac cagtaggcgc gatttctctg atttcttcta aaatacgtcc ttgtgcatca
    32761 aataattttt ctggttcata ggatttcagc caacggagta aagcatctgc atgatccata
    32821 tgttcttgat ctacaggaat cggtacttgg tgtgctcgga aggttccttc aattggttcc
    32881 ccgtcccatt cttttggacc ggtccatcct tttggtgtgc gtacgatcaa tactggccat
    32941 tttggcatca cgacatctgt tgctgtcttc aggcgagctt ctttttggat ggatttaatt
    33001 ttctcgattg cttgatccat tttttcagcc ataacaggat ttaatttttc tgaatcattt
    33061 ccttcaataa agaaaggttc ccagccaagt ccgttaaagt aatttgctaa ttctccatct
    33121 gatttacgtg ccaagatcgt tgggttggct atttttgcac catttaaatg aaggatcggt
    33181 aaaacagctc catctatcac tggattgata aagacatttg agaaccagct tccagctaaa
    33241 ggaccggttt ctgcttcacc atcaccaatc acgacagcag agattacttc aggattgtct
    33301 aatacagctc cgactgcatg tgaaagagag tatccaagct ctccgccttc atggatagaa
    33361 cctggtgttt ctggtgctgc atgagaagca atgcctccag gaaatgaaaa tcgtttgaat
    33421 aatttttgca ttcctgtctc atcttccgtt acttctggat agatctctgt gtaacttcca
    33481 tctaaataag cattcgatac catgacttgt ccgccgtgtc ctggcccttc aatataaaac
    33541 atgttcaaat catatttatt gatgactcgg ttcaaatgga cataaataaa attttggcca
    33601 ggaatcgttc cccaatgacc gattggatgt tttttgacat cttcgggttt cagtgtacgt
    33661 ctcagtaagg ggttatcttt aagataaagt tgcccaacag aaagataatt agcagcacgc
    33721 caccaagccg tcattttatc aaagtattct ttactagaat aatccataca taacactcct
    33781 tttcattatt actcttattc tagcgcagat actcccaatt gaaacctaat aagcacttct
    33841 gttcatttgt ttggatttta tatctagcca aatgaacagg tataaaaaac cacgaaattt
    33901 ttcctttcgc tgacgttttt tacattgatc caaaaaactt gtcgttatca caatggctgt
    33961 tccgcagttc atttcgctcc tcatcatgca taatttgctg aatgacgctg ggccagttaa
    34021 cacattgctg gaaaactggg gctggatcga gcaattcctt tcctgattga tccgttttat
    34081 gtaaaagtct ctgttatctt tgtcaatatg tgaattggta tcccagctac tatgctaatt
    34141 tctacaggga ttatccaaaa tttggatcgt gaccaaatcg aagcggcgta atcggaaata
    34201 tcaataactt caacgtgatt tttcttttaa ctgtcaacgg accgacaaac tcaaactttt
    34261 atggtgcagg ttccactgat cttctagtta cttggctata cctgactgtc actaaaatag
    34321 attacaatct agcttccgtt atcggtatct tgatctttat ctatatgcaa aaatttatat
    34381 actactagaa aaaagaaagt cgaatttttt acttttcttt ttctcttttt tggttagctg
    34441 acacgccgca agactaaagt catggaactc ttaggtaata cgtcttaatg aaaacaaaaa
    34501 aatcccgcaa cccctgattt cacaggattt gcgggagttt ttgattagtc tttgttcaag
    34561 tcgcgaagca attcatcgat atggttgcca tagacaactg attcatcaag ttcaaagaac
    34621 aattcaggtg tcttataaat acttagattg tgccctaact ctcgacgaat caatcctgat
    34681 gctttattca atccatcttg tgctgttttc ttatctgatg ctaagtcaga taaaatgctg
    34741 taatagatcg ttgcttgctg taagtccccc gtaactttca catctgtgat cgttacgttc
    34801 tccacacgag ggtcacggac ctttttgcgc aaaatatcat tgacttcttt taagatttct
    34861 tgaccgactc ggcggtcacg gtagttagcc ataatgattg gcctcctttt atcagttttt
    34921 agtcattttt gacttcttcc atgataaatc cttcaattac gtcatctact ttgatgtcat
    34981 tgaatttttc aatcattgca ccacattcat aacccatttt gacttctttc acgtcatctt
    35041 tgaatcgttt cagacttgca agttggcctt catagattac gattccgtca cggattacac
    35101 ggacaccgct atctctacga atatatcctt ctgttacata ggcacctgca atcgtaccaa
    35161 ctttagaaac tttgaaggtt tcacgaacga tcatttgacc ggtgattttt tcttcaaatt
    35221 cagggtctag catccctttc atcgcagttt cgatttcttc gatggcttta tagatgatac
    35281 ggtgaagacg aatatcgact tcttcagtat cagcttgcac ttttgcctgc ggtgtaggac
    35341 gaacgttgaa accgatgata atagcgttgc tggctgctgc taaggttaca tcactttcat
    35401 tgattgctcc gacagctgaa tggacgattt ttacacggac accttccaca tcaatcttct
    35461 tcaatgatgc agccagtgct tctgctgaac cttgtacatc tgctttgatg attacgttaa
    35521 cttctttcaa ttcgccttct ttcaggcttt caaataagtt atccaaagtc acacggcttg
    35581 tggcagcacg gttttctaat aatgcacgtt tcgcgcgttc ttcacccgct gcacgtgctg
    35641 tcttttcgtc ttcgaagact acgaaacggt cacctgcttg aggtacatca ttcaagccag
    35701 tgatttctac tggcgttgca ggtcctgctt ctttatcgcg acggccaaga tcgtttgtca
    35761 ttacacggac acgtccgtaa gtattaccga caacgattgg gtcaccaaca tgaagtgttc
    35821 cttcttggac aagtaaggtt gacacaggac ctttaccttt atcaagacgt gcttcgatca
    35881 ctgttccgat tgcgcgttgt gttggatctg cttttaaatc ttctacttct gcgactaaca
    35941 ggatcatttc cagcaattct tcgatgtttt ggccgaattt cgctgagatc tcaacaaaga
    36001 tcgtttctcc accccatgct tcaggaatta actcatactc acttaattct tgcataacat
    36061 gttgagggtt tgcacctggt ttatcaattt tatttacagc gacgatgatt ggaacgcctg
    36121 cagctttggc atggttgatt gcttcaactg tttgtggcat gacgccatca tctgcagcaa
    36181 caactaagat cgtgatatct gtgatacttg ctccacgtgc acgcatactt gtaaaggccg
    36241 catgtcctgg tgtatccaag aaagtgattg gtttaccatc aatatcgatc tggtaggcac
    36301 cgatatgctg tgtgattccg ccggcttcgc cgcttgttac acgtgaatgg cgtaatgtat
    36361 ccaataaagt tgttttccca tggtctacgt gtcccataat cgttacgact ggcggacgtg
    36421 agactaattt atctggatcg acttcttctg cctcgaagaa tttatcgata tcagcaatgt
    36481 caacttggac tttttcttgt ggttccatgc catagtccgt cgctaacagt tcaatcgtat
    36541 ctttatccag tgcttggttt tgattgacca tgacaccaag cataaataat tttttaatga
    36601 tttctgctgg ctcacgatga atttttttcg caatatctgc tacgttcata ccttcagtat
    36661 actctaatac atctggcaat tcacggaatt tgcgtggtgg tacagctggt ttagttgatt
    36721 gttgctgttt gcctttttta ccttttttat tataacgatt acggttatta ttattaaatt
    36781 tgccgcgatt gttattgttt ggtttgttgt tattgttagt acggttttga ttgccgcctt
    36841 ggctcgttcc ttgacgattt tggctgctgg tatttcccgt attattttgg tttgttgttg
    36901 gtttttttcc ttgattcact tgaccgctcc cttgaccgcg atcttgataa ctgcggttat
    36961 ttttattttt ttgttgattg ttcgttgttt cttttttacg ttgatcgttc ggctgttgat
    37021 tggctggttt tctgcttgct tgttgtgcag cttgttgttt cgtgcttggg tttttgaacg
    37081 cttgttgcaa tttcttctca tcattgctag taatcgcacc catatggttt ttcacatcga
    37141 tgccaagttg ttgtgctttt tccacgacgt ctttactaga ttttttgagt tcttttgcta
    37201 attcatagat tcttttgttg cccatgcaat caccttccta accttcaatt agctccttgg
    37261 tcttcttcgc aaatccggca tccattattc cgaatacctt gcgaggtttt ccgatcatct
    37321 gcgtgatttc tgcttcagaa aatagctcaa agcaaggaac ttcataatat gaactcttat
    37381 cttttatctt ttttcttgta ttctcgcttg catcggttgc cacaaacacc aattttgctt
    37441 tttgtttgcg gatttcagca atggtcagtt cttcacccgt gattagtttt cccgcgcgca
    37501 tcgctagacc gatgagattc atggcttttt gccgattaac tgtattcatt ttccaaataa
    37561 ctctttacgt gctttttggt gagtgacata gtcaagaagt tcttgataaa actcatctgt
    37621 taatgttgtt tcgagtactc gatcgagaat ttttttatcc caagcttgtt gtacttcttc
    37681 cggctcaagt gagacatacg ctcctcgtcc aggcattttt ccagttggat cgatcgatac
    37741 ttccccttct tttgaacggg tgattcggac catctctttt ttaggtttca tttcacctga
    37801 aaccacggat ttgcgtaaag gaattttcct ttgtttcatc tctttgcctc cttactaaag
    37861 ttaagcttct ggtttttctt cgaccgtttc attgttgaat gcaattgttt catattcatc
    37921 gtctgttaaa tctgactgaa cgattgcttc atcatgcagt tcttctgttt ctttttgttg
    37981 ttcttgattt tcataaaatt ccgtcatgtc agattctgat ttgatatcga ttttatggtt
    38041 agtcaattta gcagctaaac gtgcgttttg tccgcgttta ccgattgcta gcgataattg
    38101 gtagtctgga acgacaactg tgcaggcctt tggatttgtc tcatcgaaga tcacatccat
    38161 gacttgtgca ggattcaatg catttgcaat gaatacagct ggatcttcat tccattcgac
    38221 gatatccatg ttttcgcctt ttaattcatt gacgattgct tgaactcgtt gtccctttgg
    38281 cccgacacat gtaccgactg catcgatatt tggatcattt gagcgaactg ccacttttga
    38341 acgatcgcca gcttcacggg cgatgctgac aatttcaacg attccatcat agacttctgg
    38401 tacttcttgc tcaaataagc ggcgcaatag gtctggatgg ctgcggctaa caaatacttg
    38461 cggaccttta gacgtatttt ctactcggga aacataaact ttgattcgat catgtggttg
    38521 ataaaattca tttggcattt gatcttgttt agaaagaact gcctcaattt taccaagatt
    38581 gacataaata taacgtttat cctggcgttc gacgattcct tgcatgatgt ctttttcata
    38641 agcactgaat tcgttataaa taatcgttct ttctgcttca cgtacacgct gtaaaataac
    38701 ttgtttagcc gtttgcgcag cgatccgccc aaaatctttt ggtgttactt caaaacggat
    38761 cgtatcaccg atctcatatg ctggattgat taaaagtgca tctttcaatg agacttccaa
    38821 ctgtgaatcc atcacttctt ccgttacttc ttttaccgca tagacatgga tctttccttt
    38881 tttctgatca aattccactt cgacgttttg tgcttggcca tagtgacgtt tgtatgcaga
    38941 aaccaatgct gcttctaaag catcgatcac gatttctttt gagatccctt tttcagcttc
    39001 taaagcgtct aacgcattta acatttcttt actcattttt tctgattcct ctccgtgatt
    39061 aaaattggat agctaagcga gcttttgcaa tattttttcg gtcaaaagtc aattcttttt
    39121 ctcgtgtttt tatgcgtatt ttcaatgtaa gctgttcagc atcaaatgat tgaagaaatc
    39181 cttcatattg tttttcgcca tctactggtt ggtataagga gacatggaca taatcaccaa
    39241 gtgccttttc atagtcgctt tcttttttca atggccgctc tgctcccggg gaggatacct
    39301 ccaggaaata agcttgagga atcggatctg gatctgctgc atcaagtttt tcactcagtt
    39361 tctcactgac aaaggcgcat tcttcgatat cgataccgcc ttctttgtca ataaatactc
    39421 gtaagaacca gctttttcct tcttttacaa attcgacttc gacaagttca aaattttgtt
    39481 catctaaaat cggtgtcacc atttctgtga ctgtttcaac gacgctactc aaaagtttcg
    39541 cctccttaaa aaattaagtg cgtaattcca ttaaaaagag tgagcgaaaa atccgctcac
    39601 tcacagttag tatcattacc tataagaact ataccatact ctgcttaaaa ttacaagcag
    39661 acaggaaaac agatgcttat agcatatcga ataacgataa ttgattttca tctggtagat
    39721 ctttcaatac gccattgtcg ttcatatatt cgatcaatgt ttttgaaact ttaccgcggg
    39781 tggcaagatc ttctttggat aaaaatggac cgtcttttct tgcttcaacg atttgttttg
    39841 ccacgttggt tcccaaactt gggacagctc taaatggtgc gatcaacgta tcaccttcaa
    39901 taacaaagtt aacggcatcc gatttataca aatcaatcat tccaaatttg aatcctcgtt
    39961 ccagcatttc attggctaac tccagcactg tcagttgatt cttttctttt acggaagcat
    40021 ctaatccttt gtccgtgatt tctttcattg cttgtttcac agcatctttt cctttgcaca
    40081 tggagactag atcaaagtcg tctgcacgta cagaaaagta tgcacagtaa tacaaaatag
    40141 ggaaataaac cttgaaataa gcgacacgaa gagccatcaa tacgtaagct gcagcatggg
    40201 ctttcggaaa catatatttg atttttgaac aagaatcgat ataccagtcg ggcacattat
    40261 tttctttcat cgccgataga taggtttctc gcaattcatc agggatcttg ttccattgtc
    40321 ctttacgaac agtttccatg attttaaatg ccatcccgct atctaatcca gcatggatca
    40381 aataaaccat aatatcatca cgacagccga ttacttccgc cagtgtcgca tctcctcgtc
    40441 gaatcaattc ttccgcattt cctaaccata cgtctgtacc atgagatagt ccggaaattt
    40501 gcagtagttc tgcaaaagtt gtaggatgcg tttcttctag catccctcga acaaaacgag
    40561 tgccgaattc tggaatacct aatgtccctg ttttggaata aatttgttcc tgacttactc
    40621 caagtacttc aggaccggca aatatccgca tcacttccgg gtcatccgta gggatcgttt
    40681 gtggatcgat ccctgataaa tcttgaagca tccgaataac agttgggtca tcatgcccta
    40741 gaatatctaa tttcaataca ttatcgtgaa tcgagtgaaa atcaaaatga gtcgttttcc
    40801 attccgaatt ttgatcgtct gctggatatt ggatcggcgt aaaatcatag acatccatat
    40861 aatcgggaat aacgatgatc ccccctggat gctggccggt tgtccgtttg actccagtag
    40921 cgccttttgc taatctgtcg acttctgcac ttcgaaactg cagattattg tccctttcgt
    40981 atccttttac atagccatat gctgttttgt cggcgactgt accaatcgtt ccggcacggt
    41041 aaacatagtc ttcgccaaac aatactttcg tatagttatg ggcttctgct tgataatcac
    41101 ctgagaagtt caaatcaata tcgggcactt tgtctccatg gaatcctaaa aatgtctcaa
    41161 atggaatatc atgaccgtct ttgtttaagc gtgcaccaca ttttggacat tgtttttctg
    41221 gcatgtcaaa tccagaacca tacgtaccat cttcaaaaaa ctcggaatat tggcactctg
    41281 gacaataata atgaggggct aacggattaa cttcagtgat tcctgtcatc gtcgcaacaa
    41341 agctagagcc aacagagcca cgtgaaccta ctagatagcc atcttcatta cttttatgca
    41401 ctagtttttg ggcgatcaga taaataaccg agaagccatt accattaata gaattcaact
    41461 ctttttttaa tcgtttttga atgatctcag gtaacggatc tccgtacatt tgttttgctt
    41521 ttgtgtaact caattctgat atttcatctt ctgaacctgg aatcttcggt gtataaagtt
    41581 catctttgac tggaatcact tcttcgcaga tatctgcgat tttattcgta ttttctacta
    41641 cgatctcttt cgctgtttct tcccctaaaa agtgaaatgc agttagcatc tcatcggttg
    41701 ttcggaaatg gacatccggc aaactatgac gattcaacgg attcgctccg cccatagaat
    41761 tgatcaaaat ttttcggtaa atggcatctt cttcgttcag ataatgtaca ttacctgttg
    41821 caacaacgat tttatccaaa ctttttccga tttctaccaa attttggata atttcttcca
    41881 aatcatgttc attttttacc aattcttgct cgatcaaagg cgcatagacc gcttttggca
    41941 taacttcaat ataatcgtag aattttgctc ggtttcttgc ctcttctacc cctttttgca
    42001 tcatggcttc gaatatttct cccttatcac aagctgagcc gatcaataga ttttcacgca
    42061 tctttttcaa ttgggagcgt ggtattcgtg ggacgcgttc gaagtattcg acattcgaca
    42121 ttgaaatcag tttgaacaaa tctttcagtc ctgcctgatt ttttgctaaa atcgttacat
    42181 ggaaaggtcg agcccttttg taagaatctc cttctccgat atgctcattt agctgatcat
    42241 gataaagcat gttatgattg tccattgcct ctttcacaaa gatccatgct aagtgaccag
    42301 ttgcttcagc atcatagatt gctcggtgat gctgttccaa gctgacacca aattttttag
    42361 ataacacacc taatccgaaa cgtttgaatt gaggatacag atatctagct aattccaacg
    42421 tatcaatgac tggattggcc gcttcaggaa taccatacct tgcgtagcta gtattcaaga
    42481 accccatatc aaaagccgcg ttgtgggcta ccaaaattgt atcttttgaa aattcaagga
    42541 acatccgcaa tacttcttct tccgactttg atcctcgaac cattccatcc gtgataccag
    42601 tcagatctac cgttgttcgt gacaaaggat gtcctggatc gataaattca tcaaagcttt
    42661 cgatcacatt tcctttatac attttgactg cggctagctc gatgatcgtg tcatatactg
    42721 cagacaatcc tgtcgtttcc acgtcaaata cgacatatgt ggcttcactc aatgcttcat
    42781 gtgcatcatt ataagcaatt gggacaccat catctacgac attcgcttcc acgccgtata
    42841 agattttcac acctgctttt tttccagcac tatgtgcttc cggaaaggct tgcgcaccac
    42901 cgtggtctgt aatggcgata gctcggtgtc cccatttccc tgcttgtgcc acaagatcag
    42961 agatgctgtt tgttgcatcc atcgtactca tattgctatg aacatgaagc tctactcgtt
    43021 tttccccttc aggagcataa tctttgcgag gtgtatgttt gacttctatg atatcttgcg
    43081 cattcatgac caaatcacgg acaaacgtat cttcttgaat acttcctcgt acctttaacc
    43141 agctaccgac actgatcgca tcaaatactt gttcatcttt ttcgccgttc gagaattttt
    43201 tgacgataaa tgatgaagta taatctgtga ttttcaaagt caagatcttg cgttttgaac
    43261 gtagttctct gacttctttg tcgaatacgt acccctcaag tgtgactctt cgttcttctt
    43321 caacgatact gatcattggt gtcgttggtt catcggctgg aatatttcgt cctagctgga
    43381 tgtggatggg tccttcaaga gctggcgatt gctcttttct ttcctttttc ttttgttcgt
    43441 ggacaactag actttcagct gcttgcttca taaaggcttc tgcctgttct tgtttgcgtt
    43501 cttcaaaaag tttcaatact cgttccgctt gctgctcatc cacttctggt tcgatacgaa
    43561 attttgggaa tccgtaactg acgaacaact cttctactaa tggcaagtat tgttgcttca
    43621 aataagaaat agctcctgta ctgtcgattg gcaagatgac tttacgatct tttatcacag
    43681 gtacctgcgt tttcaatact tgctgtgcta gtggtgtatc gcattgctga ttttccaaag
    43741 cttgggccca gtaatcttgc aagagctgtt catcgaatgt ctggtcatct gctaagatat
    43801 tcagtttgac ttgtgcaata tctttaaaag cgatagtcag atgctgagtc aatgaccgat
    43861 atagcataat aggcaaaatt ttggaaaaat gcagcgtaaa ttcccataaa cgcgatttac
    43921 gatggaccac tacactatcc atttcaccag cagtaagcag tggatgattt ttttctgctt
    43981 cctctagttg gatttgttcc aacagttttt caaataaatc tcgtttttct gacaaagata
    44041 tacgcccttt ctgatgagaa acaatgcaat tgatcgcatc ttatcctctt ctttttcagt
    44101 accttttagt atactcgttt tttctgagtt ttacattaaa aaattggaac tgcgattcaa
    44161 atcgcagttc caattttaaa agcgaatcat cagattgtca aaaacagctg attcagctat
    44221 ttttattttc tgtgttttaa cgcttattat tcgccttggt tcaagaggat tggtaaagta
    44281 ttcaccaatt cttctttacg tacttcaacc atttcgccag ttttcttgat tttcacttct
    44341 acgattccat ccactgcttt tttaccgaca gtgattctga ttggacagcc aatcaaatca
    44401 gaatccgcga atttcactcc tgcgcgttca tttctgtcat caaccagtac ttcatagcca
    44461 gcttttgtca tcgcttcttc tagttcattc gtcaggtttg tttggtattc gtcttttaga
    44521 ttcatttgga cgatatgcaa atcaaatggc gcaattcctt ttggccagtt gattccttgt
    44581 tcatctgaat gttgttctac gattgctgat aataaacgac ttacaccgat accataacag
    44641 cccataatca caaatttttc tcgtccattt tcgtctagta cagttgctcc catagattca
    44701 ctgtaacgag tgccgatttt gaagatatgt ccgatttcga ttcctcgagt aaaggcaaga
    44761 acaccattgt tgtctggtga aggatctcct tcttgtacaa aacgaagatc ttcataagaa
    44821 agtacagtga aatcgcgttc tggattcaca tttttatagt ggaatcctgt ttcgttcgct
    44881 ccagtaatgg cattcgctaa atcttggaca taacggtctg catatacttt tacgtcatct
    44941 gaaacaccga ctggtccgac agaaccaaac tcagcaccta gatatttttg tgcttcttct
    45001 tctgttgctt cttctaaaaa gtcagcagat aaaaagtttt tcagttttac gtcattgact
    45061 tcatggtctc cgcgtaccaa aacaaggaca ggttgttcat ccgcaatgaa caatacagac
    45121 ttgatgattt tttgaggttc aacagaaaag aaatctgcta cttcgtcaat tgattttacg
    45181 ttaggtgtag ctactttctc caattcaagc tgtgtttcat gcgatttttt tgatacatag
    45241 tagcttgtag ccatctccaa gtttgcagca taatcacttt cagtcgaata acagatcgta
    45301 tcctccccga tttctgagat cgccatgaat tcttttgagt ctttcccgcc cattgcgcca
    45361 ccgtctccga taatcgagcg aaattctaag ccacaacgct tgaatacttc ggtgtaggct
    45421 ttttcgtaat ctttatatgt ctcgtccaaa ctttcttccg ttgcatggaa agaatacgca
    45481 tctttcatga taaactctct tccgcgaagc aaaccgaagc gaggtcgttt ttcgtcacga
    45541 tatttaggtt ggatttgata aagatttaaa ggtaatttct tatatgaatt gatttcatta
    45601 cgcatcaaat cagtaaaagt ttcttcgtga gttggtccaa ggatcatttt tcgatcattg
    45661 cggtctttga aacgatacaa gtttggtcca tatgtttcgt aacgcccgga ttcttcccaa
    45721 agttctgctg gcaagatggc aggcatcagc atttctaccg ctccgatttt ttcaaattct
    45781 tcccgcataa tgcgtttcag tttttccaat acacgatttg ctaatggcag atatgaataa
    45841 atacccgctg atacttggcg gatatatcca gctcttagca agatttgatg gcttaatact
    45901 tctgcatcat ttggaacttc gcgtaatgtt ggaatcaaca tttttgattg tctcattcta
    45961 tatactcctt tatttcctca aaatagttat cgtttgttat tataccctat ttgtcaactt
    46021 tttctagaaa aagaatcgtt gaatatcgtt ccatgtaact agcaccatca acaacattaa
    46081 aaatccaaag ccagctaatg tcaatattcc ttctttttct tgacttaatg gtttcccgcg
    46141 aatcccttca aaaatattca agactaattt cccgccatct aaggcaggaa tcggaagcag
    46201 attgacgatt ccgagattca ttgaaagaag cgccatcaaa ctaataacag ttgttacacc
    46261 ttgatttgct gcttctgaag acaattggta catcatgaca ggtccgccta atttatctaa
    46321 actgaaacct gtaaatagtg atcctaaggc tttgaaaatt tccaatgaac cgctgaaagc
    46381 ttgacgcgtc cctccaatga tcttatccat aaaacctgta ttcattgggg cttttatacc
    46441 aagttggcca actttttcgc catttgactc tacagatttc ggtgtcacgt cgacagattg
    46501 cacctgacct tctcgttcga tcttaaagtc taatgtttta tccggatttt tcgtaatgac
    46561 tgttgtcaga tcattccatg aatggatttc ttttccgtcc acactgacta cctcatcatt
    46621 ttcttttaag cctgcttctg ccgcagctcc attaggcatg atctctccta cgcgattagt
    46681 gttcgtcact tgaacaccac cttgcataaa tgccagaatg atgaataaca ggattgccaa
    46741 aataaagtta ttcatgggtc cagcaaagtt ggtcagcata cgctgccaca atttagccga
    46801 ttggaactgt acgtcttttg gcgcaattct gatttctgtt ccgtcgtttt caataatcgt
    46861 cgcatcgtga tcaacagggt aggttactac ttctgtttcg tctccattca cataaccagt
    46921 gatagtcagc tcatcttcta aatcataacg gctcaattcc atcgggatag catttgtcaa
    46981 ttggattttc ttacttaaat ttatcttctc tacgatgccg tcagaattta gtaaaagaga
    47041 aagaggcatt cctggtgcca tttctgtttc atcatcccca ttgcctgcca ttcgaacata
    47101 accgccaata ggtaataggc gaagcgtata tgttgtcccg tcttttgctt gatgcccata
    47161 aattttcgga cccataccga tagcaaattc gcgaacgagg attcctgatc gtttcgcaaa
    47221 gaagaaatga ccaaactcat gaacaatcac taatatacca aaaacgatga taaatgtcag
    47281 aatcgttttc atagcttttt tctcctttac ttgttttttg tgttttttat aaaacaacga
    47341 taaaacacta taattttacc atagtatacc aaagacacca atagtatcaa tataatgaaa
    47401 aataaaaacc tgcgtctttt atgaaattca caaagaaacg caggtatact tactaaataa
    47461 tgccaaataa atgcatgata ggaaatacaa acagcaaact gtcgaaacgg tctaagattc
    47521 caccatggcc tggtaagata tttcccgagt ctttcacatc ataatgacgt ttgattgcag
    47581 attcgaccaa atcaccaaat tgtccgactg ctgaaaaaat aatcgtcatc agaagcatga
    47641 caaacatacc atgattaaat agttcttttg ctgggtatag caataaataa agaaatgcga
    47701 ctacaacagc actaccaata ccaccgagcg caccttcaat cgttttgttg ggcgaaatat
    47761 caggacttaa cttatgctga ccataacgtc gaccaaccat atatgctccg atatcagttg
    47821 cccaaacaat aaatagacca aatatcaaaa cagccaagct ttctgttcgt gcattgacaa
    47881 aattctggaa acctattccc acatacaagc tgacaagcac aggaaaacct gcttcatcaa
    47941 tcgtatacgt atttttagaa ataactgatg ttcctaaaag aatcataact ataagataaa
    48001 ataacataaa tgtacttaca ttctctggaa gaaagaaaaa ccatctttct tttggcaaaa
    48061 caagtaacac tgcaccaacg gcggataaaa ctccttcaaa acttaagagt gtcaatcctt
    48121 tcatccgaaa cagctcgtaa actcctatta ctgctaagat tgctgctgta agctcaaccg
    48181 ctatgcctcc gatccatata atcggaataa acaagactag cgccacggct gctgtgacca
    48241 cacgttgttt catgactgat cccctttctc atcttctgtt ttttttacac cgccgaatcg
    48301 acggtctcga ttttgataag aggcgattgc ttcttcaaga tgcgcaccat caaaatcagg
    48361 ccaaagcgct tttgtaaaat acagctcact atatgcgatc tgccataaca gaaaattact
    48421 aattcgctct tctccacttg tacggatcat cagctcagga tcttgtaact ctttaggcaa
    48481 gaaacctgtc attaaatgct tagcaatcaa ttcatcatcg atttcttctg cgtgtatttc
    48541 tccttttgct acttcttcgg caatttcttg gacagcagtc acgatctctg ctctgcttcc
    48601 ataattcaaa gcaaaattca ataccattcc agtattgttt tttgtttgct caattgcacg
    48661 tcttacagca tcttgcgtat gttctggcaa tacgttttca taacccatga catgaacttt
    48721 gacattttct ttaatcaatt ctggtacgaa cgtatcaaag aagtcgacag gtagttgcat
    48781 gagaaaactt acttcatctt ttggtcgctt ccagttttca gtcgaaaagg cataaagcgt
    48841 caacactttc actccaagcc ttgatgcttt ttttgtgact ttctttactg tttccatgcc
    48901 ttctttatgg ccagcaactc gtggcaaacg acggttttgc gcccaacgac cattgccgtc
    48961 catgatgatg gcaatgtgct gaggaattgg tccatcggta ttaaattgaa attcgctttt
    49021 ttcttgaata tatttgtttt tttgcggaaa aaaacgtaac attctttttc ctcctatact
    49081 taaaagattc ccactatctt aagtattata gcaatatcta actgtttttc ctatgaaatc
    49141 attgtttttt tatttaaaaa ctgaataatt ttttcttttt ttaattttta aataaaagaa
    49201 atataaataa aaacagagga agcggaatga tccgcccctc tgtttttatg attttattaa
    49261 acttccaata attctttttc tttatcagca gcgatcttat ctacttcttt gatgctgtta
    49321 tctgtcaatg tttgtacgtc tttttctaat ccgcgcaaat catcttcggt aatatcacca
    49381 tttttttgtt gttttttata ttcatcgatc gcatcacgac ggatgttacg aatcgcgatt
    49441 tttgcatttt ctgcttcttt tttaacatct ttagctaatt cttttctgcg ttcttctgtc
    49501 agttgcggga taactaagcg aatgacattt ccatcattgg ttggactgat tccgatatca
    49561 cttgccataa tagctttttc aacatcttga agaatgctct tgtcaaaagg tgtgatcatt
    49621 aaaacacgtg cttctggaat ttggattgat gccatttggt tcaatggtgt tggcgcaccg
    49681 tagtagttca cagtaatacg gtccaaaagg ctagcgttcg cacggcctgc gcggatttgc
    49741 ccaagttcac gttgaagatt ttgtgctgct tttttcattt tttcttttgc ttctgtcata
    49801 attgcatctg ccatttttat ttccccctta cagttgttcc gatattttcg cctaagattg
    49861 cacggcgaat atttccgtgt tcatttaaat tgaagacaag cagtggaata tcattatcca
    49921 tgcttagtga acttgcagtt gaatccatga cttgtaatcc tttggcaata acttccagat
    49981 gagtcagttc ttcaaatttt actgcatttg catctacttt cggatcagca gaataaacac
    50041 cgtctacatt gtttttagcc atcaggataa catctgcgcc gatttctgcc gcgcgtaatg
    50101 ctgctgttgt gtctgtcgag aagtacgggt tgccggtacc gcctgcaaag ataacgatcc
    50161 gtcctttttc taaatgacgt tctgctttac ggcgaatata tggctctgcg atttgacgca
    50221 tttcaatcga agtttgcacg cgtgtcggta cacccacgtt ttccaacgta tcctgtaaag
    50281 ctaaggcatt cataactgta gctaacatac ccatgtaatc agcttgagca cgttccatgc
    50341 ccatttgtgc tccgatttgt ccgcgccaga tatttccgcc gcccacaacg attgccattt
    50401 caacgcccag ctcgtgaact tctttgattt cttggatgat ttctttgatt actggtggtt
    50461 tgattccaaa tcctgcgtcc ccagctaaag cttctccact caattttaag actacacgct
    50521 gatatttcgg tgtgaccatg ttcattcctc caatattttc tattgccatt ttaccatact
    50581 tagatgattt cgacactcta agactggttt ttcttgtttt gcttaaaaaa agggagcgca
    50641 caaaccgtgc gttcccgtct gttattcaac agaattattt tttcacttgg ctcataactt
    50701 cttctacaaa gttatcttcg cgtttttcga taccttcgcc aacttcgaaa cgtacgaatg
    50761 ttttaacagt tgcgcctttt gatgcaacgt atttttcaac agtcatatca ggatctttaa
    50821 cgaatggttg gtccaccaaa gcgatttctg ctttgaattt cttcaagcgt ccttctacca
    50881 ttttttcaac gatattcgct ggtttgcctt cgttcaatgc ttgttctgta agaacagttt
    50941 tttcatgttc taattcagct tctggaattt gtgattcgtt tacataacga ggattgatag
    51001 ctgctacgtg catagctacg tctcttgcaa ctgtttcgtc agttgttcca tctagaacag
    51061 tcaatacggc aatacgtccg cccatatgca agtatccacc aaatgcagca ttgtcgtctt
    51121 tttctacaac ttcaaagcga cggaagctga ttttttctcc gataacttga gttgcttcga
    51181 tcaagtcaga ttcaattgta cctttatctg ttttgatttt cattgcagct tccatgtctg
    51241 ctggtttgtt ttcagcaaca agttctgcaa tttcttttac taaatcttgg aacatttcgt
    51301 ttttagaaac aaagtctgtt tctgagttta cttcaacgat tgctgcagtg ttacctttca
    51361 cagcaactga agccaatcct tcagctgcaa tgcggtcatt tttcttcgca gcctttgcca
    51421 ttcctttttc acgtaaaaga tcaactgctt tttccatgtc gccttctact tcaactaatg
    51481 cttttttcgc atccatcatt cctacgccag tcatttcgcg tagttctttt accattttag
    51541 ctgtaacgtc tgccatgttt aactcctcct gtgtgttttt ctttttaaaa aaagctgctt
    51601 caaaggagag tgaggcttgt cgcccagcct ttgaaacagc tccatcattc aaaattattc
    51661 tgctgaagaa ttgtcgcctt caacaacatc tacgatttct tcgattgatg ttgctgtgtt
    51721 ttcttctgtg aaatcttctt caactacttg atcttcacct tggtttcctt cgatgaaagc
    51781 atcagccatt tttgaagtaa tcaatttaac ggcacgaatt gcatcgtcgt ttgaagggat
    51841 tactacgtcg atttcatctg gatcgcagtt tgtatcaacc atagctacga ttgggatatt
    51901 taatttgtgt gcttcttgaa cagcaatacg ttctttacgt gggtcaacga tgtacattac
    51961 atctgggatt cttggcatat cagcgatacc gcctaagaat ttttcaagac gttcacgttc
    52021 tttgttcaaa ccagcaactt cttttttagg tagaacttca aaagttccat cttcttccat
    52081 tgcattgatt tgtttcaaac ggctgatacg tttttggata gtatcccagt ttgtcaatgt
    52141 tccgcctaac caacgatggt taacgaagta ttgtcctgca cgagttgctt cttctttgat
    52201 tgcttcttgt gcttgttttt ttgtacctac gaataatgct acgccgcctt cttcagcaac
    52261 gctcttcatg taatcataag ctgcatctac taatttaact gttttttgca agtcaatgat
    52321 gtagattccg tttctttctg tgaagatata tttcttcatt tttggattcc agcgacgagt
    52381 ttggtgacca aagtgtacgc cggcttctag taattgtttc atagaaatta ctgccatgtt
    52441 tgtttcctcc gatttggttt tatttttcct cttcttatct tctaccttgc cggcaaactt
    52501 tttcaagcac ctaccgacaa tccaataaga atgtggattt gctgatgcat aaagcaccct
    52561 cagctattat acagacaaaa gatccgtatc gcaagagcaa atatactttt ctgctcaaca
    52621 ttcaatagtt tacctgaaag ataaaaagaa ttcaagaaaa acagtagatt tctctttgtt
    52681 tctttattat cctttttcca taaaataatt ttaggtataa aagacgaata ttattgggca
    52741 atccttcttt tttttgttat gatacatgtg aaatctttcg taaaggagaa agaaaatgcg
    52801 caaagctcaa agacatttgc tgattaaaca agtcattgaa gaattcccga tccgtacgca
    52861 agaagaacta ttaaacaaac ttgggcatta tggcgttacc gccacgcagg caacgatttc
    52921 tcgagatatc cgtgatctaa aaatcgtgaa ggcacctgat gaaaatggcg tttcccgctt
    52981 cgtattgttt cgcgaaaaag gaactcctga gactaggact gaggaagagg atcggctggt
    53041 tcaaatgatt gaagacattg tattgaaagt cgaacgagtc cactttttga cgatcgtcaa
    53101 taccttgcca gacaatgctc atttatttgc atctgtttta gatgagatca aacctccctc
    53161 tattgtcagt acaattgctg gattcgatac gatcatggtc atctcggaaa atgaggagga
    53221 cgcaaaacga gtagaagaat ttcttcacca tccaactgaa gtatcacttt tttaatcaga
    53281 gcacgtattc ctaatatgga atgcgtgttt ttttattttc aatattttcc ttattgaata
    53341 aacctttaca ctctttacaa aaatacatca ttttttattc aaatttttca acaccttgat
    53401 tgaatcacaa taatcatcat ttatattgga tgttaacaag agcagcttct tgctctttgt
    53461 gtatttcaaa aaagaaatga aaggaaatca tatgtataaa ttattaactt ctggaccatt
    53521 gacgacttct gaaacagtga aaaatgctat gctgatcgat cattgtactt gggatcaaga
    53581 atacaaagaa attacacaat ggataagaaa aaaattattg gatcttgctc aagtatcgga
    53641 aggaatatac acaacggtct taatgcaagg tagcagctcc ttcggggtag aatcggtatt
    53701 aagttctgtg atcaggccag aagatactgt actgatttgt tcaaacggcg catatagaca
    53761 acgaatgatc aacatgtgcg aatgtctgaa actgaactat accgtttatg aggtatcgga
    53821 acatcaaatc ctccaagctc atgtgattac acagctgatt gaagagaatc cgtctatctc
    53881 cgcactagca atggtccata gcgagactac ttccggtatt ttgaataatt tggaagagat
    53941 tgctgctgtc acacaagaaa gagatctttt gtttatagtg gacgcgatgt caagctttgg
    54001 tgggataccg atttgtgtag ccgaactagg gattgatttt ttgatttcca gctcaaacaa
    54061 atgtattcaa ggtgttccag gcttttcttt tgtacttgct aataaagcgg aactggaaaa
    54121 acgaaaaaat gttagccgaa gtttgtcact tgatctatat gaccagtacc gcgtgatgga
    54181 aaacgagaat ggcaaatggc gtttcacttc ccctacccat acggttttgg cttttcaaca
    54241 agcgctgaag gaactagagg cagaaggagg aatcgaaact cgatattcac gttatctcgc
    54301 caataatcat gtgctacgac aaaaaatgac agagatgggg ttccagcctt tcattcaaga
    54361 atatcagggg ccattcatca cctcttttct ttaccctaag gactggcgat ttgattttaa
    54421 tcatttttat cagtatttaa agcaaaatgg atttgtgatc tatcccggga aaatcagcga
    54481 tcatgaatgt tttcgtatcg gaaatattgg cgagatctat ccggaagata ttttggaact
    54541 gacagcaatt atcaaagact ataaggagaa cgttaatgat taaaactgta atttttgatt
    54601 gggcaggaac aactgtggac tttggctgta tggctcctgt ccacgctttt agaaatgctt
    54661 ttctagaaaa aggaatccaa ctgactgaca aagaaatccg ctaaccgatg ggaaaattaa
    54721 aatgggatca tatccaacag cttctttccc tcccttctgt caaaaaacaa tggatagcta
    54781 tttatggaca actacctcag gaaaaagagg tggaggaatt gtatcaacaa tttgaacatt
    54841 atctgtttaa agatttggtc aagcattcta ccttgaaacc cgacacattg gaaacgatcg
    54901 aacggttgag aaacgctggg atcaatattg gttccacgaa tggatttaat gctcctatga
    54961 tgacaatcgt agcagaaacc gcaaaaaaag aaaggtattc accagacttc atggcaactt
    55021 ttgaagacgt cgaagggtat ggtcgaccgt ttccttatat gatccataaa aacatgcagt
    55081 acttcaaaaa caagtcggtt gatgaagtaa tcaaagtcgg agatacggtc tcagatatcc
    55141 aggaagggaa aaatgccgga gtgttgacag taggtgtaat tgaaggtagt tcgctaatgg
    55201 ggatttctta tgatgagtac caaaaattag agatggggaa aaagatcgtt taaaacagtc
    55261 tgttgctctt agattccaac aagccggcgc tgatatttgt gtaaacaatt taaaagagtt
    55321 aacagaatac attttgtacg ctgacatgaa tccagaatga cttttataca aaataagagg
    55381 ttgtgaaaaa gctgtcctgt atcaagtaat aaaaacagcc aagcaaaaat agctcctcat
    55441 atttttcgtt tggttaggct tattaccgca gatgcaagct tttgaacacc gtttattcga
    55501 aaaaaaaaag ggagctgtga cacgactttt gtcacagtcc ccttatttgc aaacaaacag
    55561 tgtttaagag acaacctctc cactaaacgg tgttcattca gctgactctc ggtatttgct
    55621 caaaaaacga taaaatttga aaagctattt tctcttttgt ttctctaata ctcggatcag
    55681 aacgctgatt tcacattctc tttttcttac atcttatctg gtgcatgaag tcctagcaaa
    55741 cgaaggtctt ctttcaataa tacagtcact gcataaacta gagctaagcg tgcttctttt
    55801 tgttcatcat cagctaaaat cttcgtatga gcatagtatt tgttgaatgc ttgtgctaat
    55861 ttgattgcat gtttcgcaat cactgacggt tcgtattttt ccccagcaga caagaccgtt
    55921 tcagggtatt tttgaactaa tttaaccact tcccagctgg tatcgtcatt caatgcatag
    55981 tctgctgttt ctgaagggac aaatcctgct ttttccaaca ggctgactgc acgagcatgt
    56041 gtatattgca cataaggtcc agtttctcct tcaaatcgaa caacttcttc caggttaaag
    56101 tcaaatgtat tcagtcgatc gtttttcaaa tcgtggaaaa tgactgcccc aaccccaact
    56161 tgtttagcta cagcatcttt gttttccaaa tcagggtttt tctcgctgat ttgttcttta
    56221 gcagattgga ttgcttcatt taatacttct tctaataaga cgatctttcc tttacgagtt
    56281 gataattttt taccaccttg cgtgatcaaa ccaaatggaa tatggtgcat gtcgtcagac
    56341 cagtcaaagc ccatttcctt caagacagct ttcaactgtt taaaatgata gctttgttca
    56401 ttccctacta catatagaga ttgtttgaag tcgtaagtac gtttacggta caatgcagca
    56461 gctaagtcac gtgtaatgta tagtgtcgca ccgtctgact tcttaatcaa cgctggattc
    56521 aggtcataag cagataggtc aacgatctct gcacctttat cctcgttcag caaatgtttt
    56581 tcttctaaaa gtttgacgat ttcatccatc ttgtcattgt agaaagcttc accgttcaac
    56641 gaatcgaaac gtacttccag cagatcatag attttattga attctttcat cgattcatca
    56701 cggaaccatt gccataattg aatagcctct ttatcgcctt cttccagacg tttgaaccaa
    56761 gcacgagctt cttcatttaa ttctggttct gtttcagcta cttcatggaa ttggacgtat
    56821 aaacgaagca attcattgat tggttctgct ttaacagctt cttctgtacc ccattttttg
    56881 taagcgacga tcagttttcc aaactgtgtt ccccagtcac ctaaatggtt gatgcggatt
    56941 ggctgataac cgattttttc cataatgaaa ccgatcgaat taccgataac tgtcgaacgc
    57001 aaatgtccca ttgagattgg tttggcaata ttaggagaag acatgtcgat tggtactgtt
    57061 ccttgattcc cgatgttgct atccccataa tgttcttttt ctttcactac tgtttgcaaa
    57121 acttttttgc tgataagttc tttattcatg aaaaagttca agtaaggtcc gacaacttcg
    57181 attttttcaa agttggcaga atcgattttt tctgctaaat ctgcagcgat ttgctgtggc
    57241 gcttttcggt aaacttttgc taatgaaaag gctggaaatg caacatctcc gtgttcagct
    57301 gattttggat tttctagtaa ttgctcgact tgttccagtg ttaaatcatc tttgacagcg
    57361 tcatagacag cttttgcgac aagatcttta ttattcatct ttattcctcc taaagtgttc
    57421 ccttgattgt taaacaaaaa aaatctctag cgacggcata cttagtatgc catcactaga
    57481 gacgagattt cccgcggtac cactccaatt gtcctaaaaa aggaccagct ccatctgata
    57541 tcggtagacg accgtctgtt taaaacagaa ttctccaagt gcgcttcgtt gtatcttctt
    57601 cattcggctt ccaccacttc cgaattcgct agccaagaat gatacaagta ctctcttgtt
    57661 caacaaatct tagtcttatt aaactttctt gaattatatc agaaaaaaaa acgaaaggct
    57721 agtcgtttca taaaaaaaca atccagatgg cctaaaggag aagaaatacc acctggatag
    57781 ctataaatca tttcgtaaat acgaaagact taacaaatta atcagctacg ataatcgttc
    57841 cttcttcaga agctaaaaga ttctcgatat tttctaaaga agtgatgatt gccttagcgt
    57901 ttggttttgc ttcaacaaat gcaatagctg cttctacttt cggaagcatg cttcctggag
    57961 cgaattgctt ttcatcaatg tattgtttca tttctgaaac agtaacagtt tctaatttct
    58021 tttgatctgg tttttggtaa ttgacataca cattgtctac tcctgtcaat acgatcagca
    58081 aatccgcatc gatgatttcg gctaattttt ctgaagcaaa atctttatcg attacggctt
    58141 ctcgtccttc taatccagta gctgtttcta caacaggaat cccaccgcca ccaacagaga
    58201 ctgtgataac tccttgatcg accagcgttt cgatgacacg tgcttcttta atcgagatgg
    58261 gtttagggga agcgactact tttctccatc cacgtccagc atcttctttg aatgtcacag
    58321 tggagtcagc attcatttgt tctttggctt cttcttccgt gtagaatgga ccgactggtt
    58381 tacttggatt tttgaatgaa ggatcatttt catccacaat aacttgagtg actaaagaaa
    58441 ccacatcttt atcgatccct ttttccttca gtacttcgcc catggcgttt tgtaaccagt
    58501 aaccgataga tccttctgtc attgcaacac atgtatctaa aggcattgcc ggtgttttct
    58561 cagaatcagc agcttgctgc tgaatcaata ggttaccgac ttgcggacca tttccgtggg
    58621 agatgatcaa ttcatctcct tgttcgataa atttcactaa atactttgct gtttccatta
    58681 acgcttcttg ttgggctttt gcacttgcat ccgtagacaa gatggcattg ccccctaaag
    58741 cgacaactac ttttcgtttg gccatttcat tcgtcctttc ttcagttcta ccgaattttc
    58801 tagataacaa aacgagccag cgttttgtga ttttccctca atgctgactc gttttttcgt
    58861 gagatgaatg tggttatgct taaacgcgag ggataaacaa gttacctaat gttgctgcca
    58921 tgatcgcttt gattgagtgc atacgatttt ctgcttggtc aaattgacga gcatatttgc
    58981 tgcggaagac ttcatctgtt acttccattt cagtgatgcc aaagcgttct ttcatttgtt
    59041 caccgtaaac agtgtttgta tcatggaaag ctggcaagca gtgcaagaag atcaaacgat
    59101 ctgtgttgtg tgttttttct accatttcca tattgatttg gtaaggtttc aacaatttga
    59161 tacgttcttc gaatttatct tcttcaccca ttgataccca tacatcagaa taaaggacgt
    59221 ctgctccgtc tacaccttta tcaacatcgt cggtaatcat caattgtgca ccggattttt
    59281 cagcaaagcc ttctgccatt ttgacgattt cttcttcagg ttgtaattct tttggtgcaa
    59341 cgatacgcat attcgctcct aaaattgcac ctgtgactaa taaagagtta gccatattgt
    59401 ttcgaccatc tccgcagtaa gcaacagtga taccttctac tgttccaaag ttttcttgga
    59461 tagttaaaaa gtcagcgatc atttgtgttg gatgccattc atcagtcaag ccattccaaa
    59521 ctggaacacc agagaattct gctagttcct ctaccatttt ttgactgaat ccacggaatt
    59581 cgattccgtc aaacatacgt cctagtactt tagctgtatc ttctgtggat tcttttttac
    59641 ctaattggat atcatttgca cctagatatt caggatgtgc acctagatcg atcgctgcag
    59701 tagtaaatgc agaacgagta cgtgtagatg ttttttcaaa taaaagcgca atattttttc
    59761 cttctagata gtgatgtggg ataccacgtt ttttcaagtc tttcaagtgt tctgaaaaat
    59821 cgatcaaata ttgtaattct tcttttgtaa agtctttttc tgctaataag cttcttcctt
    59881 ggaatactga ttctttcata ttaaacattc tcctttagat tgtttttatt taagattaaa
    59941 gaggaaatcc cgacggcagc tgacagtaag actgccgtca ggaaacaaat tacttcaagt
    60001 cttcacggac aagtggttgg ctcatgcagc gtggaccacc acgacctcgt gacaattcac
    60061 ttgaattgat ttctaacact ttgatgccat agctacgaag caattcgttc gatacataat
    60121 ttcggttata agtgactaca acacctggag cgattgccaa tgtgtttgat ccatcattcc
    60181 attgttctcg aggtgcaacg atctcatctc cattaccagt tgggatcagt acaagatcat
    60241 ctaatccaag agcagctttc atcgtttcgt gtaagttatc tgaatgagta atcttgatat
    60301 catcaccatc tggttcgatt gtaaagacat ccatcttacc gtctttactt tgaattgctg
    60361 gatggatagt gaatttatcg tgatctacca ttgtaaatac ggtatccaaa tgcatcattg
    60421 cacggttgtt cggaattttg attgccaaca ctttttcaaa tcctgaattt tttgcgaata
    60481 ggtggcgtgc taatttttct aaagctttcg cattagttcg ttgagaaatc ccaacagcta
    60541 ctactttatc gcttaagatc aattcgtctc cgccttcgat atgatctgga ttttcacgat
    60601 ccaaccatac ttcgattcct ttatttgcaa atcttggatg atgtttcaaa atatattctg
    60661 taaacatcga ttcacgacga cgtgcttcaa aagtcatctt attgactgtc atcccatttc
    60721 ccattgatgc ggaagggtcg cgtgtaaagt acaagtttgg cattgggtcc atgtaaaatg
    60781 gatagtcatc gtctgctgac agatcatata gactgctaga acgtacttcg atatcttttg
    60841 tacgaacccc agccatgatt ttatctacca tttcttgcgt ttccattgat agaaggaact
    60901 catgaagacc ctctcttaca gcattcgatg caatatgaga ttcatccaac attttattca
    60961 agaattgttc tttgacattg cctgcgtcaa tcgcttctgc cgctaatttt tccaagtaaa
    61021 gagtctcgac gccttcattt tgcaatgtct tcgcaaagtt gtcatgttct tcttgagcaa
    61081 tcggaagata tggaatatca tcgaacaaca gacgatccat aatatctggt gtcaaatttt
    61141 ccacttcttg tccaggccgt ttcagtaaaa ctgtctttaa tttccctatt tcagagaaaa
    61201 cgtgaatagg tttatccatg ttgtgttgcc tccttctttt tgattacatg tataggatat
    61261 cctccttttg taatcacttt cataattttg tctcattcaa aatgagacat tcctcacatt
    61321 aatattcgat aattaaataa ttaaacgttt ttctattttt aagcgtaatt tattcatttt
    61381 ttattctgta tacactttca aaaaaatatt tattcattga aaaaattttt ttgaataaat
    61441 taaaaaaata aattatatta tcgttgttag cgtttactat atgtattatt gttgtttttt
    61501 atacacaaat cattcgcttc aaaacaaatt attcaatacc attgtatatt ttttacataa
    61561 taacaaatta tatttgtaaa gtattaaaac ttgtttctaa tgttggtatg ttaaaacttt
    61621 acaaattctt taaaaaaagt gacaataaat atagtgaaca ataaggagag gactttttaa
    61681 tgcgtgttaa taaaatcgat tttcactttt caaaactcag atatcaacct gattttgaac
    61741 atttctcgga tgaagagttc caacagatca aggaacatac tgttcttcgt tcttataaaa
    61801 aaggacagat ccttttcgac gatggtgatc ccagacaacg gttgtatatc ttgaccaaag
    61861 gactcgttcg tttagaacgt tatgatgaaa gtgcaactta ttactactat gactatgtga
    61921 atacaaataa cctttttcct atgtcaggaa tgtttggaga agagacctac acttatacag
    61981 ctcaagcgat gactgatatt gaaacattct accttcctgt ttcctactat gaaaaattag
    62041 cacgaaaaaa tcccgaacag ttgatctata tgatacaaaa aatttctaaa gtagttgaaa
    62101 tacatgaatt gcgtatccaa atcggattga cctctagcgc ctttgaccgg gtaaaacaaa
    62161 atttattcat tttgaaagaa gaattgggcg taccaacaaa agatggtcat atcaccattc
    62221 cttatccgat caccttaaaa gaattagcag tgaatagcgg cacgacacgt gaaactgctg
    62281 gacaagtaat caaacaacta cgtgattccg ggctgttaga ttatgacaaa aagcgtttta
    62341 ctttctgtaa gacagctact actccagcta tgagagaata acgcgaacaa aaataagcag
    62401 tgaccctgat aaatatcagg gtcactgctt attttcctcc ctaaaacttc tgaaagatcc
    62461 ggcagtttta gatcactgga caagactcaa agattttcta tgtgtgtttc agttatgtaa
    62521 tattcactgt ttgcaaaaaa tactgttaga ttaactagat gaagcgtctt gtcctcatcc
    62581 gatctagatt attcgatatt cctcgacaat aaacgattac tcagtgaggg caatcagagg
    62641 aacggataat gataaataag ctgtaaaaaa tagcaaaata acaataatga ttaaaagtat
    62701 gtaaaaaata gggctttgac aaaaacgtgt cacagcccta tttcgattaa acggtgtgta
    62761 cctgtcaact cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa
    62821 attgttcctt gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt
    62881 caaaagcttg catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt
    62941 cctgggctgt tcttattact tgatgcaggg cagctttttc acaacctctt tattcagata
    63001 aaaccaagct tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca
    63061 catgtgctgc tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca
    63121 ctagttctac ctcagggagt ttagctgcca aagcatcctg caatccttca cgcaaccctt
    63181 gattggccgc tacacctcca gctataatca gttgcttgac tggataattt tgacaagctc
    63241 gaagtgtttt agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat
    63301 ttttgtctag atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt
    63361 ttaatccgct aaaactgaaa tcataattat cttcatggat catcgctcgc ggaaaatggt
    63421 aattatcttt tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac
    63481 ctaatactct tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa
    63541 tgatttcata tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa
    63601 gaagtgccat caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg
    63661 ccatatggtt tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag
    63721 aaataccaat cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata
    63781 aatcttctgc cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt
    63841 ctacgtgatg gcggctagct acttcaggta ctacgccacc aaaacgttta tgactgttta
    63901 tttgagaagc aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg
    63961 tttcatcaca acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt
    64021 ttttaacttt ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg
    64081 tttacctaat atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag
    64141 acgtacttct aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa
    64201 caaatgccgt gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc
    64261 gacttcatct attactttcg agtatccaat aaagccacag atctggttat cttcagctaa
    64321 aagcagataa tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg
    64381 tgaaccatgt ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc
    64441 atcaaaatct ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat
    64501 atccaacgca tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa
    64561 ccccaatttc cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat
    64621 cgtctcacag cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat
    64681 tcgtttacgc tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa
    64741 cgctcttgaa ccgataaaac caatcattct tccattatca aagataccta gatatagatg
    64801 aggaagcggt gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt
    64861 atagacttct cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag
    64921 tacgtaacga cgatcgattt gttcgactgt tttttctgta taaatcattt tatcttgaaa
    64981 aaagcttctg gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg
    65041 tctccaacca tttttcttct gcttctactc gttttaaata gcgaggaaga aaagcttgtt
    65101 tctctgcaac tggttcaact aaagcaccta attgagccac agttacacca cttggaatat
    65161 cccatgctgg aactgtatta atcatcgcac taggcaatat ttcctttatt tcagagaaga
    65221 atttgtgaca atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga
    65281 acaaatcaga agcagaaata tgtacatctt caagtacagt ttctaatcta ttttctttcc
    65341 agcggtatgc cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataatat
    65401 tatccacacc gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt
    65461 tatctaatgt ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag
    65521 aaccgggacc atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg
    65581 caaacaattg gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt
    65641 gtatttgtcc aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag
    65701 tatctattcc taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca
    65761 ttgtagcata cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaataat
    65821 acatgattat ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt
    65881 atcactccat tttcccacag cttttccatc ttttatttcg ttgattctcg aataatcaac
    65941 tctgtttcca caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc
    66001 gtctccactg ctagctctcc catccattcg gtataaactt ttacagtaga aagcgagggc
    66061 gttacatatt tcgctacact gacatcattg aagcccatca cagaaatatc ttctggtacc
    66121 actaaacctt tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca
    66181 aaaagcgcat caggaacatg cggattcgtt tttaaatatt gcttcactgc ttgatatcct
    66241 gactcgacag aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa
    66301 acttgttgaa atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat
    66361 tctgttccag caagcatagc gatatgttgc gctttatggg agacaaagtg ttcaatgact
    66421 ttttgaacac ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca
    66481 gaatctacca aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg
    66541 aattttccta gtgcgatcgt tccatctgcc ggaagatcac taatggaatc ccacgtttct
    66601 ttcaataaag taacgtttgc ttcttctgcc tttttttcga ttcctaggcg aatagataaa
    66661 taataaagat cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc
    66721 ttagtatttg ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa
    66781 attttttgct ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa
    66841 acagtagcag gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc
    66901 tcctcctttt ttacgtttat aaaatcgcag taaatcgttc aaacgctgcc tgtccttttt
    66961 catctcgctt gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt
    67021 tttgtacgac atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt
    67081 ctgcccacgg acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta
    67141 cttctactag ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca
    67201 acccaatgtt ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag
    67261 gatgttcttc tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc
    67321 ttcttcgtgc aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga
    67381 tagatactgt ttcatccgaa tatgctttcc atttttccaa tactgagtta gctgcttcaa
    67441 taagcaagtt tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc
    67501 ctgcgatgac ttgcggataa tcttctagtt tgataagttt ttctatcggt gcttttgcca
    67561 taggaaattc atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa
    67621 ttggtaagtc agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca
    67681 gtctagcaaa tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg
    67741 aatgttcatt gtaataagca taaggggaat attggaatcc ccagctttca ccgtctagat
    67801 tcattcgaat aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt
    67861 cgttttccat gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg
    67921 cgatttgttt tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat
    67981 actccgactc tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat
    68041 ttttcttact taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct
    68101 gtgcaaagaa tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt
    68161 caaaaatctc ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga
    68221 caagctgatc caataagtct acagattctt ccttaactgg tcttacttca atgctttcta
    68281 gagcatcttc tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca
    68341 tccatccgcc agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca
    68401 tatctcattc ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt
    68461 tttgataatc gctttgatat ctgtgtattc aggtttccag cccagaatct ctctcgcttt
    68521 ttcacttgaa gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg
    68581 aatttctttt cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt
    68641 gctgctgcct aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa
    68701 aatatgtgca gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg
    68761 tgtatcataa tcgtctccat aaacagccaa ggcttttctt tgaccaagag ctacttgtaa
    68821 aatgattggg acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt
    68881 tgctccggct acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca
    68941 ccacttcatc attttttcca tcatcaattt actttcacca tacggatttt taggattagc
    69001 tggtgtatct tctgtgattg gagactcttt cggctctcca taagttgctg cagtagaaga
    69061 gaatacgatt ttattcacgt tgaactcgtg catgacttca agtaagattt gcataccgta
    69121 tacgttgttg ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc
    69181 agcaaaatgg attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt
    69241 atcccggatg tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt
    69301 gagcaaatta tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg
    69361 cgaaccgata taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat
    69421 tagtaaaatt gtgtttgttg attctatata atttaaaaaa cgaagtttac ttttatggtg
    69481 aaaacaaaca aagtaaaatt tcgtaaattc atatggtaaa tttataataa aacatttagt
    69541 aaacattttc aagggttttt tgcaaaggtt tacaaacaaa cgtaaaacta gccttttttt
    69601 ctcttttgtt ccataataga tataacaaat aataaatgag gtggattcaa tgaaaaaatt
    69661 tgtatctgga atattagtag gaagtctggc aacagccgct gccgttgctg gtttggttgc
    69721 ttctgtgaaa aaaacagtga tcgatccaat tgacgaaaaa gaagcaatga tcgaagaaaa
    69781 ccgtaaaaaa gcaatgcgta aacgcgtttc tcgttaataa ttatttaaaa atacgcatga
    69841 agactttcta aaaaacagat aagtcatcat gcgtattttt atttgtcagt caatataagt
    69901 tgtgctattt tatctgcctg tctcagattc catggatctg tataaggtct agctactgct
    69961 gcagttagcc aatccacacc ttcaacaggt attccatagc aataaaagtc agactggatt
    70021 tcccctgaag catcaatcac gcgattcgta cctcgctcaa catctatagc ttctgaatga
    70081 taacttcctg tattatctga aaatgaaaaa gggcgaacaa gtttgttttc cttcaaattt
    70141 tttatgacag gattacttga acgagaaaag cttgtttgtg gcaatcgagc ttcaatcaaa
    70201 tagcgactgc tgatttcttt atcttgtaga gaaataacat agcggttgtg cttttgctga
    70261 atatcaaatg gcggttcgat caaatgaaaa atacccgcat ctatcagtgc tgctaattct
    70321 ttcgtccgaa tgattggcgg accgatcgtt aaaaagctat ttagatgagt aaaccatccc
    70381 catagtattt ctttgtattc cttagcagaa aaaacgtgcc attcaatagc ctgccggatt
    70441 ggatcacgcc agtcttttaa tgcatcaaat gttgaagcaa atgctcctgt gcaattgcct
    70501 ttttccgctt cttttatctg cccttcaata aatattcggc tagcttctga aaagggctca
    70561 ttctgatagg caagaggagt ttctaaatac gaccaatccc atctataagg gattagtgct
    70621 ggatgctttc cgagacactc ctctgaagag agggagagaa aatcatgctc aaaatttttc
    70681 tttggaatcg gcaagtgata ttcttcgatg attttcttgt aataaaacaa ctcaacgtct
    70741 tttttcagat agtcaaaaaa tgtttcgcca gtcaaaaatc ccgactgatg ccattgctcc
    70801 aggttttctt ttgttagtaa ccgtggccat gccattgctc cgccttgctt ttgatttctt
    70861 cctcgagggt aataagggag cccccttctt gatccactgt atacaatcgg ttccttgcct
    70921 gatggatgat agaccagttt tcccttttcg ttctcgaagc gccctcctct tgatactgta
    70981 aacaatccga cataatcaaa aaaacttaag cctagacctc ttaaaaagac attttcttta
    71041 ggcggtatct tcgaaagatc tacatctgca ggattagaag gaagctgata cagtaaaaca
    71101 tttttctttg cgtagtctac ccacactctt tcttcttctg tctcatcatt ttcccagtga
    71161 ccgcttgcta aaacaagctg atcgatagaa tagctttgct gatctgtctg taaaagataa
    71221 ccatcttttc ttttgcttac tttttttact aaagaataaa caaactgaat agaagctgga
    71281 aattcttctt gcaactttga gaaaaaccag cgttgataga gtccgtataa acatcgagta
    71341 gattgctgat ctttttccag acgattgatt tcttctataa ttagttgctt atttttggtt
    71401 accgtctgtt cgatataatc tgtagcaggt cctttactcc attgaaacaa gttgggtccc
    71461 tgtgttacca atcctccact ggacaatgtt tcatctgtaa aaagcgtcac ttgttgagaa
    71521 acagaattca ttaataactc cggtggctga ttcaaccgcc aaactcgacc tccaggacca
    71581 ttgggatcga aaagaaaaag ttcaagagaa tgtctgtcct gattattatg gagaatcctt
    71641 tctgctacac ttaatcctct aggtccggca ccaatgatcc ctattttcat ttgcatgcct
    71701 ccatttcgat aaattttttc aataaaacag catggttctg atgttcattt tttgcaccat
    71761 aaacaaaagt aatgactgga tgttctttca taagttgact gagtttaaaa atagcttgct
    71821 tcttttcttc ttcgaaaatc aattcttgct ggtatttctt tgcaaatgaa gcaaatcgtt
    71881 ctggtacatg gtcaaaagtt ttgcgtgctt cattactagg tgcaatttct ttcaaccaca
    71941 gatctaactg cgctttttct tttgaaattc ctcgtggcca gaatctatcc acgagtattc
    72001 tatagccatc ctctttttca gcatcaaggt aaacacgttt taatttgatc atagttgttt
    72061 ccttctttct accttttcgc ttagcttata tatgtagata atagcacaaa ataaaaaaaa
    72121 tgaacggcac aactatccat tgcgccgttc atgtgatctt ggtcaaaatc taatgattga
    72181 ttcggttttt aaagctgtca tacaatcatc tgttttatac ttttttactt ttgatttttc
    72241 cagaccatcc gtcatatcca cccttaagta aataaatctc ttgataacct gctttacgta
    72301 atttatttgc tgtacggatg ctcaaacttt tcttttgatc gtaaatatag acaggctggt
    72361 cttttcgaat agatcctaat gaatctttca aaacagtgta agggatgttt cttgcaccta
    72421 aaatatgccc agcatcaaac acatcttttt cgcgaacatc gattacttgt gcttttctca
    72481 ttccttctcg aaattcttct tctgtcaaag tcgttgctga acgtttggcc attactcgta
    72541 gatagacttc ccaaaggatg attgcaagca caatcaatag caaaatgata ttaattaccc
    72601 ataaaaccat gtaatgcctc cgttttttta agcaaatttc aaggctaatg aggcagcacc
    72661 aatgacacca gcttcattac caagctctgc taatttgatt tttgttgatt ctcttacttg
    72721 cgggaaagta aattctttaa aatatttctc tacacggcta cgtaaaaatt ctcctgctgc
    72781 cgatactcct ccacctaaca cgatacttga cggatttaat gtattcccca agtttccgca
    72841 agctaaacct aagtagaagc atacgcgatc aacaaccatc aatgcaaacg gatcgtcttc
    72901 ttgagccatt tcaaagatat ctttacttgt tacttcttct ccgttgtcta gcattgcttt
    72961 cagttttgaa tcccctgcgt attcttctgc caagaaacga cctaggcgta ccacacctgt
    73021 agcgctagat acagtttcta aacatccgcg tttaccgcaa gtacattcga acccttcagg
    73081 atccacagtg atatgtccga tttctcctcc agctccgctc acgccgtgaa tcaaatttcc
    73141 gccagcaacg atacctccgc caacacctgt tcctagagta acgaatacaa cgtctggatc
    73201 gttttctcca gcacctttcc atcgttcacc taaagcagca acattcgcgt cattatcgat
    73261 agcgaatttg attcctgttc ctgcctcgat cagttttttc acaaattgaa cttctgtcca
    73321 gtttaagtta tacgcaccaa taaccgttcc agcttcgctg tccacgcttc ctggggtacc
    73381 catgccgatt ccgataaagt cttcggcttt catattatat aaattcaaac gatgattgat
    73441 tgattcaatg atttcaggaa cgatatgctt accatcatcc aaaatattgg tatcgatgct
    73501 ccatttttgc tgaatctctc cttctggtgt taaaattgca aattttgctg ttgttccacc
    73561 taaatcaatc ccaataatct tcttgtccat tacaaatttc ctttctttcg gctttcttca
    73621 attcggtgct ctctttttaa tatgcgccac gctgtcatgt atgtatcatg atcaatcaaa
    73681 cggctatcat acagattacg caattcgata gtcattaatt caatgtcata gatgcgtgca
    73741 cccacataga taaagatgcc aaattttttg aacagctgct gtacatcata taagctctcc
    73801 atctttaagc acttccttca tattatacaa gtaaaccata tttttttaaa cccagtaaaa
    73861 tacatattac aaaaagaaat acaaaaacta gtgtagaaag aatcctttgg tgtatcgaat
    73921 agcttgtctg ttgtttcggt agacccaaaa cattccctaa cactaatccg ccaattagcc
    73981 caccgacatg tccccaaaca tctacagagg tatccagaag accaaaaaca aatgtcaaca
    74041 caataaataa aagaaaatgt ctgactagct gctggatcgc tgggttatgt ttaaaatgaa
    74101 accctaagat aaacaacgca ccgaacaaac cgaatatcga agtacttgca ccaccagaca
    74161 gaacattcgc ttcgttaaaa gcaaagcttg ctgtatttcc cattatcccg ctgaacatat
    74221 aaatcaagaa aaaacgccaa tggccataga ttgcctcgat ttgctgcccc ataaaataca
    74281 gtatcagtga gttcattacg aaatgcatca gtccaaaatg gatgaagatt ggtgtcacta
    74341 aacgccacca ttcattgaag tgaacaagat aaggaccata cattccggca taataaggga
    74401 tctcaagtcg cggaaataat tccatcatga ggaagaccgc tgtctgtatc cctaaaaaag
    74461 cataggtcca taacggtcgt cttaaccatg cacgcaattt tatttgctgt tgataattca
    74521 tgaaaacctc ccatttcatt ttagtcaata aacaattgtt ggatcttctg atcaaagctc
    74581 tccggttgcc attgttcatt caattgttca cgaaaaacaa ggctacatgt ttttttatga
    74641 aaattattta aataccggtc gtaataacca ccgccaaaac cgattcgata accggctcta
    74701 ttgtagataa tacctggtac aatgaggaga tcgatcatct ccggcaaata gagcttctct
    74761 gattgtggct caagaacacc aaaactcgaa cgtacaagaa cggactgttc atccatttca
    74821 taaaacgcca tctgacgttt cggcaaagtt ttaggtacaa ctattttctt ttgttctttt
    74881 tctgctcgtt ggataaccgg ctgtgtatcc agttcaaatt cattcgataa agtcgtccct
    74941 atcacttttg ctgacttcca gtactcgctg gcgaatagct gtttatagat tcgctgttct
    75001 ttttcctttt tcttgccact tttggccaga tttttcagct tttcgattcc tttccggcgt
    75061 aatcttactt tcaattggtc caaaaactca cccttccttc tctcataaaa tggtatagga
    75121 aaaaaagaaa aatagcaatc gaactttcaa ataaatgcat aaatagaata aaaagaagac
    75181 ctagaaatga ttggccttcc ttttttaatg aatttcaact gttcagcgcg tgtttttgtc
    75241 tgataaaaag tgcatacaga aaatatggcg caccaaaaag ggcaatcaaa attcctgtag
    75301 gtacttcccc acttggtaga atcactctag caaccatatc tgcactaagc agaaaaatgg
    75361 aaccgaacaa accgctcaga acaaaggtcc agttattttc tttacgtacg actagcttcg
    75421 cagcatgagg agctagtagt cctacaaaag ataaactacc tgtcaccgct acactaccgc
    75481 ttgccaaagc aacagctaac aacaaaagga ttttttgact tttagccacg ttaaggccta
    75541 atccttgcgc gcgttcttga ccaaatgata gcaatacaat cctctgtgca taaagtcctc
    75601 ctagcggaat taagataacc gcccatggaa gcaatgccca aacataaggc cagctcgcac
    75661 cccagattga tcccgctaac catgcattca caaatccata attttcctta gataattgga
    75721 tcgtgcatag caatgttaat gcagaaaggc cggcattaac ggcaataccg gacaataaga
    75781 ttcgattcat acccaaaaag ccatttcgat cataggccgc aagatagaca cagcaagcag
    75841 ctaaaacacc acctagaaag gctacaacta gcaatagaaa cggcagcgaa tagtgcgaaa
    75901 agaaacctaa ataaagcatc actaaaaatc ctgctccggc attaatgccc aatatactag
    75961 aatctgccaa ttcatttctt gttactcctt gcaagatata accagaaacg ccaagacaag
    76021 ctcctgctaa taaactgatc agtaatcgag gaactcgaaa ttctctaaca atcagcgcca
    76081 aagaagatga ttctttccct atgaacgtgg caaaaagctg atccaggtaa ataaaaggcg
    76141 taccaatcat taaactacct atagacagga tgacaagtac aaaaagcaag aaatagaacg
    76201 tccaatatat ttttttcacc gttgttccct ccttacttgt atcagtaaaa acggaacgcc
    76261 aatcaatgca atgactaatc caaaaggtgt ttcaaaaggg ggattgatca tcctcgctgt
    76321 aaaatccgcc aacataacca atccaccgcc aactaaaaaa gagacaggta gaacataacg
    76381 atagtccgtt ccaaccattc cgcgaacaac tggcggaacc aacagcccaa taaatgaaac
    76441 aggtccgact aacgaaacgg caatggctgc taacactgca actagacaga aaacaatcaa
    76501 ccgcgttcgt ttaggatgac ttccaagagc taatgcatgt gtttcactca aatgaatcaa
    76561 agatattgct tttcctaatg aaaaagaaac caaaacagca agtatatata aaggaaacaa
    76621 tactgctaat tgtttccatg tgatattggc agctccgcct acaaaccaaa aagcaagatc
    76681 ctgctgcaga ttaaagaatt gggtaagtgc ttggctgata gcagtaaaaa aagaactaat
    76741 agctacgcca gctaaaatca gctgtaaagg attcatgccc attttcgaaa actgcaaaaa
    76801 accaaatacg agcaaagtta cagcaaaaga acctgccaat gacattagaa acacagaaaa
    76861 atcagaagct ttaggccaaa aagcaaaact aagggccatt gccaacgaag cacccgcgtt
    76921 gattcctagt aagcctgaat ctgctaaagg atttcttgtc accccttgca tcaatgcacc
    76981 gctcaatgca aaacaactcc ctacaagaaa tgctcccgcc acacgaggca aacgaatatt
    77041 tcgtatgatt tggtgacttt gttttgatct atcaaaatca aacaacgcct gccaaaaatc
    77101 tgctttttcc acagtcgctg cgccatttga aagagcaaca aaaaaaataa tcaaaagaac
    77161 aaaacagacc aaaagcagga gtctattttt cttcatagtc ctcataattg atcctcctgc
    77221 tcttttggca acaaatcata ggttagaaaa actggtttat ttgttcttgg aagcgtgaca
    77281 atcgttgcat caatctcaaa tatttttttc atacactctt ttgtgatcac ttcttcaggt
    77341 gtaccttctg tgatcaactt accgtttttc attgctaata aataatctga aaaacgtgaa
    77401 gcatgattaa tgtcatgaat cgacatcaag atcgttgttt gtttcatttg attgatttca
    77461 gtcaataact gtaggatctc taattggtgt gccggatcta aaaatgttgt cggttcatcc
    77521 aagatcaaga tatctgtttc ctgagcaagc gccatagcga tccagacacg ctgggtttgt
    77581 ccaccagaca gcgttgcgat atcccgcata cgaaatccag ctagacctgt agcatccaac
    77641 gcccactcaa ttgcttcatg atcttgcgtg tccatgccac tgaacagttt ttgataaggg
    77701 tatcgtccgt aagatactac ttcttctact gtcatacctg tagggtgttc actagtttgt
    77761 gctaataatg caattttttt tgcaagttct tttgaactgt atgtagagat atcagagtta
    77821 ttgacaatga tctttcctga tttcacaggt agtatacggg taaaactttt caataaagta
    77881 gattttccac ttccatttgg cccaatcaaa ctagtgattt tcccttcagg aatcgtaaca
    77941 gacatgtttt caatgatttt tttgttttcg tagccggctg caatgtcagc tgctcttaat
    78001 ctcgtcattt cacccatcct ttcgctgaaa atcattctca attatgacgc tgtttcaaca
    78061 tttcgtcaag cgctactttc atatctatag ccaaatgaag cggttgacag cgttcgaaat
    78121 atcaggaata ataaccaatg agaaagattc tcaattatgt taggaggaaa gaaatgaaat
    78181 taaaaacaaa agtattcaca ttatctagca tattgctgct ggctggagta ctgagcgctt
    78241 gctccccctc ttcagataaa gaaacagcta caactgccaa ttcgacgact tcatccaata
    78301 gtactcatac agtcacagat acacttggac ataaagtaga gattccaaac gcgcccaaac
    78361 ggatcatcgg cagttactta gaagactatc tgattgcact aggggaaaaa cctatcgccc
    78421 agtggactgt tggcagtggt tccattcaag attatctgca agatgattta aaggatgtcc
    78481 caacgatttc ttatgacctt ccttatgaga aagtgttaag ctttgagcct gatttgctgt
    78541 tgatttcttc ttccgctact gtcgaaggcg ggaaatacga acaatatagc aaaatcgcgc
    78601 caacttatgt tgtaaaaaac ggaacagatg tcacatggga agaacaacta aaagatgtcg
    78661 gcaaagcttt ggcaaaagaa aaagaggcag aaaaaatcat ttccgattat caaaaatcca
    78721 tcaagaaaac tcgagaagaa ctagcagata aaatcgagaa caagacagct gcagtgttgt
    78781 gggtgaccaa caactcagcc tttatggtca gcaaaaatcg gtcaagtgga agaatcgtat
    78841 acgacgatct ggaatttggt gttcctgatc tagtggaaga ggtctccaaa aaagccactt
    78901 ctgactggtc tgctgtttcc tctgaaaagc tagctgaatt agatgctgac tacatcatcc
    78961 ttgtgaacag tgacaaagat gctgccatgt tcaatgaacc aagctggaag aatctaaaag
    79021 cagtgacaga caatcatgta ctgcaattcg gacctgaatc tagttggcta tataacggac
    79081 ctattgccag tcaggaaatg gtcaatgata tcaaagaaaa actaaattga ttttaactaa
    79141 aaaaagcatc ttcacgatta gtcgtgagat gctttttttc ttattaacaa actagacgca
    79201 tagattttat tcttcttttg aagcaaccct attttttact ctttcccatt tttgaaaacg
    79261 atgggcatat agattttctt gatccgttat cccctcatac tcctctataa gggcgaagtc
    79321 tgaaaaatca atattcccaa tatacgtatc gccttcaaac gtgctatcga tcaatgttcg
    79381 ccagatccga ccagtgtaag gaagaagttc ttgaaaaatc cgactaccac ccgaaacata
    79441 gatatcttct gagatcgttt gactatacga caaaagttca tcgatcgaat gcattacttc
    79501 cgcattttct ttcgtgcgaa aatttggttg cgttgtcaac acgatgatat ggcgataagg
    79561 aagtgaaaga tcccccattc cttcataggt ctttcttccc atcactaaaa ttcgatccat
    79621 tgtatgttcg cgaaagaaac gcatatcatt tggcaatcgc catggtaaca aaccattttt
    79681 gccgatcaga ccatttttat cttgtgccca catggaaata aacatattat ccgctcctta
    79741 tgattttaga tgttttgaga taaaaaaagt ctcaattctt tgaccagcaa ggaattgaga
    79801 actttaaaaa cattattttg tttcacggtg caaagtaact ttgcgttcgc gtgggcaata
    79861 tttttgtttt tccaaacgat caggattgtt acgtttgttt ttgcttgtta gatagttacg
    79921 ttctttacaa gaagtacact ctaaagtgat gtttacgcgc atgtttttcc ctcccatttt
    79981 tacaagaatc ctctaagatg actctcagac ttgattatct taccatgatt ttttctagat
    80041 tgctagtgtt tttcacgatt ttgttaagta aaactacttg acgataaata caacggtttt
    80101 acttctcaaa caaaatcttt ttctttttgt ataaataccc ttacacttgt ttatatagat
    80161 tatgtttttt aataaagggg gatactaata tgccatggaa catgaaggac tttcctgcat
    80221 caatgaaaaa cttagataaa ttgacaagaa aaaaagcaat cgacatcgca aatgcattat
    80281 tagatgaagg gtaccctgat aaccgtgcga tcccaattgc cattgatcaa gcaaaaaaat
    80341 ggtatgaaga tgctagtgaa tcagaaaaac gaactttcga aaaagaaaaa aatccaagca
    80401 agacggatga acacgatacg aatcctcgtg ctggaaaact gcttgattca gatgtgatcg
    80461 tagaatatga ggaagaacaa tggatcgtga aatctaaagg ggcaaaaaag gcaagcaacc
    80521 attttgatac aaaaaaagaa gcaatcgaaa aaggaaagca agtagcacaa aacaaagaat
    80581 caactttgtt catttataaa aaagatggga caaaagaaaa agaaatcgat tattgaacat
    80641 atgtaaaggg gttgtgacga aaatcgtgtc acaaccccct tatgttcgaa acaatggtgt
    80701 tcctctgcca actccgaacc ataagtcata atatctataa aacgggaaga gctgtttttt
    80761 gaaattgttt cttatcactc agtgttaaac ggtaggagaa aacctctttt ttattgatat
    80821 ttgattttca tcaaacagtc acatagtcga ttgcttctgc ttgcggaatc gtttcatcta
    80881 caaagtagac tttataccat gtcaagaaac tgaagattgt ccagattttt ctttgctctt
    80941 ctttacgtcc ttcgtgatgg tcatctagca acttcatgat tttttcttga tcaaaaaatt
    81001 cttttgcaaa atctgcttcg aataatgctt tgacttgttc gtagccgtgt tcttctttca
    81061 accaagcttt gattggcaca gggaatccta atttcacacg attcgaccat tcttccggaa
    81121 gatgtttgtt cgcagcttgt ctgaaaatat cttttgtgtt gttttgattc aataaaaact
    81181 tacttggtat ttgttgagcc aatttcatga cttcgatatc caataaaggt actcggactt
    81241 ccaaagaact tgccatagaa agtttatctg cctttaacaa gatatctttt ggcatccatt
    81301 gatgaagatc gacatattgc atcttattga cttcatcttt gattccttct gttttctcgt
    81361 aatgaacagt catgatttca tccacagatg gtgctgtttg atattttggt gttaacactt
    81421 ctgatgcttc tgattcttcg aacactcgag catgaccgat aaaatattct tttgcaggag
    81481 caagtgattc atataaatgg atccgtccat ggaagttttt catttttccg attttacgtc
    81541 ctaaattata gcgtgttcct tttggtaatt ttttcaatcc ttgagcaatc acacggatga
    81601 attttgaatt cgtatggaat ccatacgttt ggtatcccgc aaacagttca tccgcccctt
    81661 ctccagattg gactacccgc acactttgtg aagcaagatt tgctaaaaag tacaaaggta
    81721 cgcaagaagg atttgaatca ggttcatcta agtgatactg gatcaaaggg aatgctttga
    81781 acgacatgtc tccgtcgatc actgctgctg tattatctaa gtccaatagt tcggttagtt
    81841 ttctggcttc aatcgcttca ttataggttt tatcatcaaa cccgattgag aaagaatgat
    81901 ctggtttcaa tacagaggtt acataactgg aatcaacccc gctggataag aaagaaccaa
    81961 cttctacatc actgaccgta tgtgcttcaa tagaagcttg aacggtttcg tcgatttttt
    82021 cgatccattc ttgtcgggaa tgcgtttctt ttgtttcaaa atctgcatcc caatattgct
    82081 gaatgtctat ttttccatct ttatacgtat aataatggcc ttcaggcaaa cgatagacat
    82141 ctttgaagaa tgtttcgccg tttaatggag aatattggaa agtcatataa ggtttcaacg
    82201 cttctttgtt caattctttg ttaaaatcag gatgaggcag aaaacttttg atttctgaac
    82261 caaacataaa tgttccgttc atttctgcat aataatatgg tttgatccca aagtggtctc
    82321 ttgcaccgaa taactcattc ttttcattat cccaaatagc aaaagcaaac attccgcgga
    82381 tcttttgcaa taattctgtt ccccattctt cgtaaccatg caaaaggact tctgtatctg
    82441 catgtgtttg aaaaacgtgg ccagctgcaa tcaattcttc tctcaaaggc tggtaattat
    82501 agatttcgcc attaaaaata atgatctttg tccgatcttc attgtaaata ggctgagtac
    82561 cgccctctaa gtcaataata cttaatcttc tgaatcctaa agcaacatgt ttatcgatat
    82621 attctcctga actattaggt ccacggtgaa cgattcgatc catcattgta ttaataattg
    82681 tttttttgtt gtccttgtcg ttaacaaaac ctacaatacc gcacatttaa ctttcacatc
    82741 ctaatataaa attactgttt atttcattca tatgcatctt atcatagaat aaatagcgga
    82801 cgaatatttt ctctcttaca atttcgtcac gtgaaaacaa aagatgaaag tttctgtttt
    82861 tacagaaaat aaagtcccaa tatctaattt accatgtttt cttttttgag gctaccttat
    82921 tctcttcgtg ttaaaaaaat attagtggat gctatttcca aaaaaaccag caaaaaaaga
    82981 cacgacctaa agatcgtg
//
LOCUS       DACDXE010000003        72303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.003, whole genome shotgun sequence.
ACCESSION   DACDXE010000003 DACDXE010000000
VERSION     DACDXE010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 72303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.003"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..933)
                     /locus_tag="IWM23_000170"
     CDS             complement(<1..933)
                     /locus_tag="IWM23_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882848.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEE"
     gene            complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="IWM23_000171"
     CDS             complement(1188..1697)
                     /gene="mreD"
                     /locus_tag="IWM23_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAP9882849.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="IWM23_000172"
     CDS             complement(1701..2555)
                     /gene="mreC"
                     /locus_tag="IWM23_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAP9882850.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(2710..3348)
                     /locus_tag="IWM23_000173"
     CDS             complement(2710..3348)
                     /locus_tag="IWM23_000173"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAP9882851.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(3349..3999)
                     /locus_tag="IWM23_000174"
     CDS             complement(3349..3999)
                     /locus_tag="IWM23_000174"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAP9882852.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="IWM23_000175"
     CDS             complement(4012..4911)
                     /gene="rsgA"
                     /locus_tag="IWM23_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAP9882853.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="IWM23_000176"
     CDS             complement(5076..7145)
                     /gene="pknB"
                     /locus_tag="IWM23_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAP9882854.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(7142..7882)
                     /locus_tag="IWM23_000177"
     CDS             complement(7142..7882)
                     /locus_tag="IWM23_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAP9882855.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="IWM23_000178"
     CDS             complement(7895..9253)
                     /gene="rsmB"
                     /locus_tag="IWM23_000178"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAP9882856.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(9253..10191)
                     /locus_tag="IWM23_000179"
     CDS             complement(9253..10191)
                     /locus_tag="IWM23_000179"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAP9882857.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(10184..10675)
                     /gene="def"
                     /locus_tag="IWM23_000180"
     CDS             complement(10184..10675)
                     /gene="def"
                     /locus_tag="IWM23_000180"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9882858.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(10690..13098)
                     /gene="priA"
                     /locus_tag="IWM23_000181"
     CDS             complement(10690..13098)
                     /gene="priA"
                     /locus_tag="IWM23_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAP9882859.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(13351..13656)
                     /locus_tag="IWM23_000182"
     CDS             complement(13351..13656)
                     /locus_tag="IWM23_000182"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9882860.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="IWM23_000183"
     CDS             complement(13661..14275)
                     /gene="gmk"
                     /locus_tag="IWM23_000183"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAP9882861.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(14762..15046)
                     /locus_tag="IWM23_000184"
     CDS             complement(14762..15046)
                     /locus_tag="IWM23_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882862.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(15064..15954)
                     /locus_tag="IWM23_000185"
     CDS             complement(15064..15954)
                     /locus_tag="IWM23_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAP9882863.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(15974..16192)
                     /locus_tag="IWM23_000186"
     CDS             complement(15974..16192)
                     /locus_tag="IWM23_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882864.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(16162..17862)
                     /locus_tag="IWM23_000187"
     CDS             complement(16162..17862)
                     /locus_tag="IWM23_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAP9882865.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            18129..18902
                     /locus_tag="IWM23_000188"
     CDS             18129..18902
                     /locus_tag="IWM23_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882866.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="IWM23_000189"
     CDS             complement(18983..19552)
                     /gene="thiT"
                     /locus_tag="IWM23_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAP9882867.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(19618..19720)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19726..20697)
                     /locus_tag="IWM23_000190"
     CDS             complement(19726..20697)
                     /locus_tag="IWM23_000190"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9882868.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="IWM23_000191"
     CDS             complement(20791..22164)
                     /gene="glmU"
                     /locus_tag="IWM23_000191"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAP9882869.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(22667..23485)
                     /gene="purR"
                     /locus_tag="IWM23_000192"
     CDS             complement(22667..23485)
                     /gene="purR"
                     /locus_tag="IWM23_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAP9882870.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(23652..24476)
                     /locus_tag="IWM23_000193"
     CDS             complement(23652..24476)
                     /locus_tag="IWM23_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9882871.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(24449..25129)
                     /locus_tag="IWM23_000194"
     CDS             complement(24449..25129)
                     /locus_tag="IWM23_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9882872.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(25152..26090)
                     /locus_tag="IWM23_000195"
     CDS             complement(25152..26090)
                     /locus_tag="IWM23_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAP9882873.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(26335..27186)
                     /locus_tag="IWM23_000196"
     CDS             complement(26335..27186)
                     /locus_tag="IWM23_000196"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAP9882874.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="IWM23_000197"
     CDS             complement(27340..29913)
                     /gene="mprF"
                     /locus_tag="IWM23_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9882875.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            30054..30860
                     /gene="proC"
                     /locus_tag="IWM23_000198"
     CDS             30054..30860
                     /gene="proC"
                     /locus_tag="IWM23_000198"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAP9882876.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(30939..32495)
                     /gene="rny"
                     /locus_tag="IWM23_000199"
     CDS             complement(30939..32495)
                     /gene="rny"
                     /locus_tag="IWM23_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAP9882877.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(32906..33955)
                     /gene="recA"
                     /locus_tag="IWM23_000200"
     CDS             complement(32906..33955)
                     /gene="recA"
                     /locus_tag="IWM23_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAP9882878.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(34064..35305)
                     /locus_tag="IWM23_000201"
     CDS             complement(34064..35305)
                     /locus_tag="IWM23_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAP9882879.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="IWM23_000202"
     CDS             complement(35545..36123)
                     /gene="pgsA"
                     /locus_tag="IWM23_000202"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAP9882880.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(36332..37216)
                     /locus_tag="IWM23_000203"
     CDS             complement(36332..37216)
                     /locus_tag="IWM23_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9882881.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(37994..39280)
                     /locus_tag="IWM23_000204"
     CDS             complement(37994..39280)
                     /locus_tag="IWM23_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9882882.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(39273..40541)
                     /locus_tag="IWM23_000205"
     CDS             complement(39273..40541)
                     /locus_tag="IWM23_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAP9882883.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            40793..41254
                     /gene="mscL"
                     /locus_tag="IWM23_000206"
     CDS             40793..41254
                     /gene="mscL"
                     /locus_tag="IWM23_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAP9882884.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(41314..41760)
                     /locus_tag="IWM23_000207"
     CDS             complement(41314..41760)
                     /locus_tag="IWM23_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9882885.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(41853..42098)
                     /locus_tag="IWM23_000208"
     CDS             complement(41853..42098)
                     /locus_tag="IWM23_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882886.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(42214..42792)
                     /locus_tag="IWM23_000209"
     CDS             complement(42214..42792)
                     /locus_tag="IWM23_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9882887.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(42869..43399)
                     /locus_tag="IWM23_000210"
     CDS             complement(42869..43399)
                     /locus_tag="IWM23_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAP9882888.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(43403..44263)
                     /gene="rlmB"
                     /locus_tag="IWM23_000211"
     CDS             complement(43403..44263)
                     /gene="rlmB"
                     /locus_tag="IWM23_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAP9882889.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(44256..44651)
                     /locus_tag="IWM23_000212"
     CDS             complement(44256..44651)
                     /locus_tag="IWM23_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9882890.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(44648..46060)
                     /locus_tag="IWM23_000213"
     CDS             complement(44648..46060)
                     /locus_tag="IWM23_000213"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAP9882891.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(46057..46593)
                     /gene="cysE"
                     /locus_tag="IWM23_000214"
     CDS             complement(46057..46593)
                     /gene="cysE"
                     /locus_tag="IWM23_000214"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAP9882892.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(46915..48375)
                     /locus_tag="IWM23_000215"
     CDS             complement(46915..48375)
                     /locus_tag="IWM23_000215"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAP9882893.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(48545..49702)
                     /locus_tag="IWM23_000216"
     CDS             complement(48545..49702)
                     /locus_tag="IWM23_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAP9882894.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(49736..51106)
                     /gene="radA"
                     /locus_tag="IWM23_000217"
     CDS             complement(49736..51106)
                     /gene="radA"
                     /locus_tag="IWM23_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAP9882895.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(51169..51696)
                     /locus_tag="IWM23_000218"
     CDS             complement(51169..51696)
                     /locus_tag="IWM23_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAP9882896.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            51926..52732
                     /gene="proB"
                     /locus_tag="IWM23_000219"
     CDS             51926..52732
                     /gene="proB"
                     /locus_tag="IWM23_000219"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAP9882897.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            52729..53976
                     /locus_tag="IWM23_000220"
     CDS             52729..53976
                     /locus_tag="IWM23_000220"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAP9882898.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            54052..54396
                     /locus_tag="IWM23_000221"
     CDS             54052..54396
                     /locus_tag="IWM23_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882899.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(54513..54839)
                     /locus_tag="IWM23_000222"
     CDS             complement(54513..54839)
                     /locus_tag="IWM23_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882900.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(55454..56656)
                     /locus_tag="IWM23_000223"
     CDS             complement(55454..56656)
                     /locus_tag="IWM23_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9882901.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            56773..57288
                     /locus_tag="IWM23_000224"
     CDS             56773..57288
                     /locus_tag="IWM23_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9882902.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(57487..57933)
                     /locus_tag="IWM23_000225"
     CDS             complement(57487..57933)
                     /locus_tag="IWM23_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9882903.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(57980..59458)
                     /gene="msr"
                     /locus_tag="IWM23_000226"
     CDS             complement(57980..59458)
                     /gene="msr"
                     /locus_tag="IWM23_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9882904.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(59812..60441)
                     /locus_tag="IWM23_000227"
     CDS             complement(59812..60441)
                     /locus_tag="IWM23_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9882905.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(60425..60889)
                     /locus_tag="IWM23_000228"
     CDS             complement(60425..60889)
                     /locus_tag="IWM23_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9882906.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(61376..62644)
                     /locus_tag="IWM23_000229"
     CDS             complement(61376..62644)
                     /locus_tag="IWM23_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAP9882907.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(62875..63612)
                     /locus_tag="IWM23_000230"
     CDS             complement(62875..63612)
                     /locus_tag="IWM23_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9882908.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPERMSVSNMIIRPTTQEV"
     gene            complement(63679..64248)
                     /locus_tag="IWM23_000231"
     CDS             complement(63679..64248)
                     /locus_tag="IWM23_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAP9882909.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            64376..65248
                     /locus_tag="IWM23_000232"
     CDS             64376..65248
                     /locus_tag="IWM23_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9882910.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            65590..66462
                     /locus_tag="IWM23_000233"
     CDS             65590..66462
                     /locus_tag="IWM23_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9882911.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            66459..68417
                     /locus_tag="IWM23_000234"
     CDS             66459..68417
                     /locus_tag="IWM23_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9882912.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(68749..69600)
                     /locus_tag="IWM23_000235"
     CDS             complement(68749..69600)
                     /locus_tag="IWM23_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9882913.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            69723..70190
                     /locus_tag="IWM23_000236"
     CDS             69723..70190
                     /locus_tag="IWM23_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAP9882914.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(70453..70935)
                     /locus_tag="IWM23_000237"
     CDS             complement(70453..70935)
                     /locus_tag="IWM23_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9882915.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(71035..71898)
                     /locus_tag="IWM23_000238"
     CDS             complement(71035..71898)
                     /locus_tag="IWM23_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9882916.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        20267 a        15493 c        12935 g        23608 t
ORIGIN      
        1 ttcttctgtt gaagattcag gtgttgttga ttcttctgtt gctgaagatt gtgtagctga
       61 tgatgattct tcagtcattg aagattgtgt agctgatgat tcttctgttg ctgagctttg
      121 tgcagtagat gaagcttgag tagcttgtgc ttcagcctct gcttgttcgc gtgcttcttt
      181 ttcagctttt tcttgtgcag cttgttgacg agcttgttct gctagacgtt gttgttcacg
      241 aatacgagct tgctctgctt cagcttctgc tttttgacgg ttcaagtcag ctttcttgtc
      301 ttcagcagtt gcttgttcag cagctaatga agttttcagt acgttcaaat cagcttgttt
      361 agataaaaga tcgccttttt gagattccaa agcagcttgg ttttcagcta attcttttag
      421 tttagcatcg ttttcagctt ttttgtcttc tacagctttt ttatcttgtt tttgttgttc
      481 catcaagtcg ttgtttgctt ttaccatcgt tgtcattgct tgaacgcgtc caatagcatc
      541 tgctaatgaa tcagcattca atacagcatc gatgtagttt gagcttgtgt tgcttacttg
      601 tgcttcgcgt gcttgttttt ggattgtatc ttcacgtttt tcaatacgtt cttgtaaatc
      661 tgcaatgtct tttactaatt gtgcagattc ttgacgcaat gtatcttgtt ttgctaatag
      721 gtcttgtgct tgtgtgttga tttcagaaac ttggctttct aaggcatcga tttgtgattg
      781 tgcatctgct tgttgatttt tcaagtctgc aatcttttga tcttgctgtt gtatctgaga
      841 atcaaaatcg tccgccgcgg cagcgatcgg tgatgctacg gcagtcaagg tcattgaaca
      901 taccattact gctgatatta aactcttttt cactcttcat tcctccgact ggcttaattt
      961 aataaatatc taaatatatt taagtctttc ttaacgatat aaaaattgta ccatagcagt
     1021 gtgtcaacga tataatagtt atgttacaaa aatgtttcaa aacaactttt aaaaatgaca
     1081 aaactgttcg tttcagaaaa tgatcatgaa aaagccgcgt taagtctagc atttcttaag
     1141 ataacaacga ttctttttcg ctttcgacct aaaaataaaa aaagaaatta ttcatcactg
     1201 aataatttct taaagggaaa aatgaataat acaaatataa tcatgttcaa cagcagtgta
     1261 ggtcctaaaa accttgtaat aaaatacgtg ttatttacta ctgctaattt aaaaatcaac
     1321 tggaccacca tcgtaaacaa ttcataaccc gtgacaaaga tgatcatact gaaaaattca
     1381 gtaaagatgt tgacatggat aacgtctttc attacataca tcaaccatac aattaaaggg
     1441 agagctactg catagatacc gataacgcca atatagtaag cgtcatagat agccccgaga
     1501 acaatcgtgg taatcagtaa ataacgtttt tcaaacgcca tactgcaaca taataatgcc
     1561 aatatcagaa agtgggcatt tgacatatag gtgcctttcg accactcccc tagcattctt
     1621 gttaaatgac catctatcaa catcaaaaag aacaaaacga ttggcagata gtacttcatt
     1681 gtttctttct tcaacatgga ctaatcctcc gctagccgtt ggacaatcgt cacaactgac
     1741 acgtcataca tttctgcata aggtttcaca taaacttccc gatccagccc ataactatct
     1801 ggtttcgttt taataaccgt accgatcggc aagttagctg gagaattccc tccaagaccg
     1861 gatgtctgga caacatcccc ttctttgata tccgtatcac cagttaattg ggtcaccact
     1921 aaggcatgga gcttttcatc atagttttta agcaaaccaa acgcttcgcc attagccgaa
     1981 gatacccgta ctggaaaatg attggagctt tcattagagg atgacagtaa ttcgatttta
     2041 gacgaagccg tattgacctc gatcactcgg ccaatcaatc ctttttgcga catgactgcc
     2101 atatttgctt cgataccgtc tttagaaccc ttgtctacga tcagcatgtc ctgccaactg
     2161 tcaggcgaac gagtaatgac atttgccgtt actttttcgt aactagtcag cgtctgattc
     2221 aactcaagtt cttttttcaa cgcttcgatt tctctctcgt aattcttgtt ttgctgtgaa
     2281 acgtcatcat agctatcgat tttttctttt agccgctcat tttcggcata agttgaaaaa
     2341 agattatgga cagaatctac tccatttcgt acccaccgta ctggggcaga gattgtttta
     2401 tcaaccattg caacactgtc attaacgatg gattggaaga aattgggctt tccttcattc
     2461 gctcgctgcg cagcggtcac acttagtatc gtcaccacga tgataacaat aaccaaggta
     2521 ataataatat ttttattagg attgaacttc ttcacaaaga cacctcgaat taattagact
     2581 cttgtgttca attttatcac taaatgccgt gaaacaaaag gtaagttgct cctcttaagc
     2641 ttttttttag caaataatag attctgtcat ttttagcctg aaagacaaat ttttttattt
     2701 gtttatggat caatctttgc tttgaataac tgcaatcata ccatctgaaa aagaaaattc
     2761 tgcggtatct gaaagaaatt cgttacttgc ataagatgtc ggatattcta cttctttatt
     2821 ttccaataca tacttgctgc cttttaatgt aagattcgag acgggagtca agcagcaata
     2881 agctaaatac ttcatatctg gtatctgtct tactatatgc tctccaggca aataataaga
     2941 caaataattt tgacaatctc tgataataag ctgatgggca aagggtttga agcgaggttc
     3001 catacctaac cagagattgg ctaaaagatg atccaatctt ccgcccgtag ctccaatcaa
     3061 agtgacttcc gcttgcgggt aacgttcaaa tgtttttaag atacctaact gtgtatctgt
     3121 atcgtctttt tctgcagggg aacgggtata ctctttggct tttgcaaata cttccttctg
     3181 ctcttcgttc gacaatgaat caaaatcacc cactgccaga tctactgggt agcctgcttg
     3241 aagcaggtac agcgcaccac ggtctatccc gacataggca tcgtatttgg acagcagaga
     3301 ctgcggccag ttttgcggat tacctccagc agctaataaa attttcattt agtctaatgc
     3361 ctcttttaat cgttcgattt gtccgactgg attttcggct ccatagatat atgatcctgc
     3421 aacgaagaca tccgcccctg catctttaca gatttttgct gtttctggaa cgattccgcc
     3481 atctacttcg attgcataag tccaattgtt ctctctacgt agatcagcga gttccgtaat
     3541 tttttctact gtttcttcaa taaagctttg tccgccgaac ccaggattga cagtcatgac
     3601 tagtacttga tcagccaaag gcaaaacatg tttgatcatg gagactggtg ttcctggatt
     3661 gataactact ccagctttaa ctcctaagct ttttgtcatt tgaagtgccc gatgaatatg
     3721 aggagttgct tcgacgtgga cagtaatgat atctgctcca gcttttgcaa atgctggaat
     3781 gtagttttct ggatcaacga tcatcagatg gacgtctaac ggtaatttag taacaggacg
     3841 gattgcctga acaatatttg gccctaatgt gatatttggg acaaatcggc catccattac
     3901 atctacgtgg atgtaatctg cgcctccttt ttccactaac tcaatgtctc tttgtaaatt
     3961 ggcaaaatct gcactcaaaa tggatggtgc tatcttcatt cttgtttcct cctattttcg
     4021 tttaccttct ttttttgcat acattggttt tcttttttcg atttcttgta agaattgcaa
     4081 ataattgtcg taacgtgtct gtgcgatacc acctgtttct actctatgtt ttacttcgca
     4141 gccaggttct ttagcatgca tgcactctct aaatttgcaa tggacagaag cttctacaaa
     4201 ttcaggaaat tgtctcggca gttctcctgc ttcaattgtc aaaaagtcaa tcgagctaaa
     4261 tcctggtgta tctgctacta acccgccata gataggaatc agttccacat gacgtgttgt
     4321 atgccgtcct cttccaagtg catcagaaat ctctcctgtt gctaaattca gttccggtga
     4381 tatttgattc agtagcgtcg attttcctgc tccagactga cccataaaga ccgatagccg
     4441 ttctgggaag taacggatca gttctgtcgt atcacccgta tgattgggta caatcaccgg
     4501 atagcctatt ttagtatagg tttgcttgat ctcttcgatt ttttctgggt cgtctgacaa
     4561 gtcggtcttt gtcaaaaaaa taatcggttc gatcgactcg tattccaagg taactagaaa
     4621 gcggtcaagt agattataag aaaatgcagg ctctacgata cttgtgacaa tgattccaag
     4681 atcaacattt gtgactggtg ggcgaactaa ttgatttttt ctcggcaaga tttccagaag
     4741 atagccgtct gtttgatttt cactctcaaa gatcacctga tctccgacaa gcggcgtgat
     4801 tttccgattc cggaaatttc ctcttcctct tgtttgatag gtctctccgt ttgcgtatac
     4861 atagtaaaaa ccgctgattg cttttctgat ttgtcctttg ataagtgcca cctccgtttt
     4921 tgtttatttt accatacgaa gaggaaagta ggtgttattc catgcaaact tctcaaagta
     4981 tatggcttga aaattgatca aaagagatag aaacacaaaa aagagaatgc aacaaatcaa
     5041 tgtcgcattc ccttctgata tattttattc agcacttatt ttgaactttc tgaggtgcta
     5101 gctgatgaac ttgtggagct ggaagattct tcttttgaag agttgcttgt gctactggag
     5161 ctacttgagc tgctagaact gtctgaagag tctttttcag aactagaaga cgctggatct
     5221 ggtcctttag agtaaattac gttcacaacc attcctttgg taattgtcgt tcctgcccct
     5281 ggtactgtac gaatcacttt atctttatcc accgtgtttg aatattcgga agtctcatgc
     5341 ccgatatatt ctgcaccgac actagctaaa taactttgtg cttcgctttt tgtatagccg
     5401 gagatatcat tcaatttagg ctctgttcct tggcttacga caaaattgat gtaagtttct
     5461 tctgctacaa cttcacccga ggctggatcc tgactgatga tcgaacctga tggtacagtg
     5521 tcgctatagt cataagtagc actaatatta ttgatattga atccttgtgt aacaagccaa
     5581 ttacgaacag tttcgtagtt ttctcctaca tgactactca aagtcactgt ttctggtcct
     5641 ttgctgactg tgaaagtaat cgtgtctgtt ttaggatcga cttctgtatc ttctgatgga
     5701 gattgcgaaa tgatcattcc gctttctacc tctgaactga attcctcttc tttttgaatc
     5761 aagtcagcag aaaaccctaa ttctttcaaa cttgcttcgg catcttcata acttttacca
     5821 ctgtaatcat ctaatttgat ttttttcgtg ccgctactaa tatacagagt gacttcgcgt
     5881 ttttgcttga ctgtcgttcc cgctgccggg tctgtcttca ctacattacc ttcttcgatt
     5941 ttgtcatctg caatttcttc tgtttttgtt gctacttcta atttcgcttt tgcgagtgtt
     6001 tctctagcag aagcttcaga aagatcagaa acatcaggaa caacaacttc tcctcgcccg
     6061 ccagaagcaa agaatacagc tgttccgatt cctaaaacag caagaatagc taataggatc
     6121 agccaccatt ttttctgtcc tttcttttta ggatcaggca gaacttcttc ctcctctggt
     6181 aaggtttcgt tttctggctt gtcttttggc agaggcatag atttgaatga atcaggcatt
     6241 ggtgcatctt ccgtaagggg tgtgataact tttgtttcat tatccatcac atgaggctgc
     6301 catttttctt cgtttgcacg agctggtgat aatactgtag aaagatcatc agacatttcc
     6361 tcggctgact tataacggtc tgcaggttct ttagccgttg cccgtaaaat cacgttttcc
     6421 agtgcttgtg gcacatttgg atcaagtgct tttatagatg gaagatcatc ttgaaaatgt
     6481 tttaaagcaa ttgttacagc agattcacca tcaaacggta cgctacccgt caacatctca
     6541 taaaggatga tccctaaagc ataaatatct gactgcttcg ttgccatact tcctctggcc
     6601 tgctcagggg acagataatg aacagatcct agcatcgtat tcgtctgcgt gatcgatgtt
     6661 tcagataaag cgatagcaat cccgaaatcc gtgattttta caacaccttc attatcgatc
     6721 agtacgtttt ggggtttcaa atctcggtgg atgatttgat ggttatgagc taaagaaata
     6781 gcagaaagga tctgctgcat aatatttact gctgtttcat aaggaacagg ataatgtgtt
     6841 tggatatatc gtttcaagtc cattcctttg acgtattcca tcaccagata ctgcatgttg
     6901 tcttcttcac caacatcgta aacactaaca atgtttggat gaacgagttc tgttgctgct
     6961 aaagcttctc tctgaaatcg tcgaatcgca gtctggtcat tttgaaagtc aaatcgtaag
     7021 actttaactg ctacatcacg atccaagatt aaatcgtgcg ccaaaaatac atttgccata
     7081 ccaccgctac cgatatttcc agtgatttga taacggccat ttaatttttt gcccaactcg
     7141 atcatacact gtcctcctta tattcgataa gaagaacggt gatattatcc gctccgcctg
     7201 cttcattggc acgatcaatc agctcagtta ctttatgaga caatgggcct tcttgattga
     7261 tgatcgttcc tatttcttct tcagacagca tgttcgtcaa gccatctgaa cataacagca
     7321 gtcgatcttt tagttgccaa agatgagtag aaacatctac ttcgactgtt ccaggcatgc
     7381 cgacagaacg tgttaaaata tttttccttg gatggttcac tgccatttct tcgctgattt
     7441 ctcctgattt gactagttca ttgactaaag aatgatcctc cgtcaattgc atgattttat
     7501 cttcacgaat caaataagct ctgctatccc caacattggc aatcgtgaac cagtcatctg
     7561 acagagcggc cgctacgatt gttgttccca taccgttata ttccggctgt tcttgtccac
     7621 gctgataaat cagcatattt tcttcttgaa tcgattgaat gaaccattgg gcgatttttt
     7681 catcgtcaac agcttcttgt ttttcccaag cttcgcctag attcgttacc gccatctgac
     7741 tggcaatgtc tccggcacga tgaccaccca tcccgtctgc taatacagca agcttgagac
     7801 ctgaacggct ggtaaataca ctggcatagt cttgatttgt gttacgtcgt cttcctacat
     7861 ctgattgata ttcaatctgc attttttcac ctcattactt tttgcgcaaa caacaaatga
     7921 aaaatccatc tgtgtaaaac tgatgcggat acaaagtcaa cattttctct tcgattgatg
     7981 attgtagcat aggattggtc aaaacatcaa tcctctcaaa atcagggtgt ctttccaaga
     8041 aagcagaaac aacgtcttgg ttttcttctt ttaagatcgt acaagtgcta taagtcagaa
     8101 taccggaaga ctttaaagtt ggtgcgcagc tttctaaaat ttccaattgg atttttggta
     8161 aattcgctaa ttcatccggc tgttttttat aacgaatatc tggttttcgc cgaagcagcc
     8221 caagacctga acaaggcgcg tctactaaaa tccgatcaaa ctgctctgct tcgaactgtt
     8281 tactgacttc tctagcatcc atcttttgag cgtagacagc agaagagaca cctagacgtg
     8341 ctgcattatc ttcaatgagt ttgactttgt gttcgtgtac atctaaagcg accacttctc
     8401 caccgcatga cggatctaaa aaagaagcaa tatgcgtcgt cttacctccg ggtgctgcac
     8461 aggcatctaa gacatgatgt tcaggctcga tttgcataga cggcgctaca agcatcgaac
     8521 tttcatcttg gatcgtcatg acgccatcgt ggaacaatga gctagaagct aaatgccctt
     8581 ttttcgctac tataccgtat ggactgactt ggcttttttc agctgtgatc tcttcttctt
     8641 gcaaaacagt gatggcctga tctctggaaa gtcggcgtgt atctactcgg gcactagcat
     8701 ggctaggctg atatagcgat aaaccgagct tttcagtttc ttcgtatccg atttgatcca
     8761 ccagtcgttt cgtcatccat agaggcaggc tgattttgac agaaagtctt tcgataggat
     8821 cagcaatgcc ttgcagatct ggtgctcctt gacgctgata attcctcaag acaccattta
     8881 caaatttccc tgtcccagga tttcctttta cttttgcgat ttcgaccgcc tcgtttaaaa
     8941 tagcatgtgc agggacttta tctaaaaatt ctaattggta tacagaaaga taaagaagca
     9001 tcttgaccca gtcatccact ttttttgctt tagcgatcaa tggtgcaacg taaaattcca
     9061 atagtcgctg atggctaatc gtaccataga tcaattctgt aaataaaccc acatcttttt
     9121 gatttagctc gctttgtttg atcatctcat tcaacaaaag attcgagtag gcgccaccct
     9181 tgttcactcg ctctaatgtc agtaatgcca tatatcgtac tgattttttc atttttttag
     9241 ggattttttt cattatttca cctgctgacc aatctgaact tgctgaccgc ttccgttaag
     9301 gaaatcatgt acggcctgtt ttcctttacc agctggctgt aattcattga tggctaagac
     9361 ggtctgttta ccgcaagcaa tccatagatt ttttttgtct ttccggatga tcgtaccagg
     9421 tgctttgtct gtagtttctg ccaatacttc aaccgataat aatttccagc gtatgccctc
     9481 ataggtcgtg aatgcaattg gccaaggacg cattccgcgt acttggttgt caatttcttc
     9541 tgcagtttta tgccaatcga ttgcttcttg ttcccgtgtg atgtttggcg aaaaagttgc
     9601 ttctgactcg tcttgaggta ctggtttttg tccatccaaa atattgggca atgtttctag
     9661 caatagcttt cttcctaaaa ggctaagctt ttcaaacatc gtccctacat catcttgttt
     9721 agtgattggt atgctttctt gcgcatagat tccgcctgca tccatttttt tgatcatttc
     9781 catgatcgtt acaccagttt ctttctcccc attcatgatg gcataatgaa caggcgcacc
     9841 tccccgatat ttaggaagta aagaagcatg gacattgatt gcccctaatt ttggtacttg
     9901 caataacttt tcaggcaaga attggccaaa tgccgcagtg acgatcacat caggcaccaa
     9961 ttcaatgatt tcttccattt ctggcgaacc ggagattttc tctggctgca gcacacggat
    10021 accgtgcttc agagccgctt tttttaccgg tgtcggcgta attactcgtt tccgcccaac
    10081 tggacggtcg ggctgtgtta ccactgcctg gatctcgtaa cctgattcta tcagcccttc
    10141 aagaatcggc acagaaaaag caggtgtgcc cataaatact agcttagtca tcaagatgtt
    10201 cctccatata ttcttcaagt tcttcttccg ggatctgctg tatcattttc tcaatgaaca
    10261 agataccgtc taagtgatcg atttcatgct ggaaggcacg ggctaaatag ccaaaagccg
    10321 tcacttctat ctcttctccg tctcgatcat aataacgcac ggtcacttca tctgctcgct
    10381 tgaccgttcc atagacatgc ggaatactga gacaaccttc tacgtcaagg ctttcacctt
    10441 ttgcttcgat gatttctggg ttaatcagtt caaatttctc gccttcatct acttcgatca
    10501 cagcaattct cttgttttgt cctacttgag gagcagcgat ccctattccg tcattcgcaa
    10561 tcattgtctc atataaattg tctaataaag cgattgtctc gtccgtgatt acatcgattg
    10621 gctgagcagt tctttttaat ttatcattag ggtggatcaa tataggatag cgcataattt
    10681 ggccctttct taaataaagt ttaatggttc gttgtcaatt gccacaaaaa gaccttgtcg
    10741 ctgttctttc tggcttccat tcaaaatttc atccaaaaca tgaggaagat tcggttcatg
    10801 tttatatttg atgattagtt gataataata tcggtttttc actctcaaaa tcatgctggg
    10861 cgttggccct aatagcaagc tttgagggct taacacttct tttagctgat tggcaatctg
    10921 aaagattttc tttgccgcta cttgttcttc aggatggctg gcggttattt tcacagtaaa
    10981 gtaataagga ggatagcctc cacgatgccg tacatgcatt tcctgctgat aaaactgctc
    11041 ataattttgt tttttagcca attctatggc gtaatgttga ggattgaatg tttggatcac
    11101 gacttcgcct gctttttccg ctcgccctgc tcgtcctgaa acttgggtaa gcaattggaa
    11161 tgtatgctca ctagaacgaa aatccggtaa attcagggag gtatcagcat tcaacacccc
    11221 aactaatgtc acttctggaa agtccaaacc tttagcaatc atttgtgtcc ctaataatat
    11281 atctgcttct tttgcaccga atttttgtaa aatctgttcg tgtgcccctt ttcttcgagt
    11341 ggtatctaca tccatacgca aaatcctagc ttgcggataa agttctcgga gttcctcttc
    11401 tactttttga gtacctgttc catagtagcg gattttattg ccgccacaat taggacagcg
    11461 attgggaata ggttcttcat gtccgcaata atggcagcgc attgaacgtg tatccatatg
    11521 cagtgtaaga gagatatcgc aattaggaca tggaagaaca aatccacagt ccctacacat
    11581 cacaaaagac gaatatcctc gacgattgag cagtaggaca gtttgttctt ttttgtctaa
    11641 tcgattttgt attttttcct gcaaattagc agaaaaagta gaggtccgat gattctgaaa
    11701 ctcttcacgc atatctacga tctcaatagc tggaagctgt gcagctgctt tggctcggtg
    11761 gtttaactgc aataactgat aaacgccttt ttgagctcgt gctcttgatt ctaaagaggg
    11821 cgtcgcgctt cctagaacta ttgggcaatg atgatattta ctgcgccaaa ttgctagatc
    11881 tctcgcatga taacgcggtg tctcatcttg tttgtaactt gattcatgtt cttcatcgat
    11941 aatgatcagc ccgatatttt tcagcggggc aaaaatagca gatcgtgcgc ctactactac
    12001 ttgcgcttct tcccgctcaa tcttgcgcca ttcatcatat ttttcacctt gggaaagtcc
    12061 gctatgtaag accgcaactg attctccgaa gcgtcctttg aatcgctcga ccatttgcgg
    12121 agtcaatgcg atttcaggca ctaacataat agccgtttta ttttctgaca aaacatcagc
    12181 gattgcctga agatatactt ctgttttccc gctgccagtg attccctcca gcaaaaagac
    12241 ctgatctttt tgctgctgac ctgctgtgat gatttgttcg acagcatttt tttgttctgc
    12301 attcagttcc aatgccgtcg tttggtcaaa tactcggtct ttataagggt cgcgataacg
    12361 ttctgattct ataaactcca gccagccctt ttcttttcct tgattcaaga cagcagtact
    12421 gattccttta tttttgaaat aagcaattgg ttcttcttgg ctcagttcag ataaacacaa
    12481 aagaagttct ttttgtttct tggcattttg ccgtaatttt gcccattctt cttcgatttg
    12541 cttttcagtt aacaaagaac ggaccaaacg aatcgttttt actttatttc gtgtatggac
    12601 ttcatatttg atatctacta attgctcttg gcgccagcga atcaattgag aaaggctgcc
    12661 ttcttcttct gcttccttcc aggaaatcac ttctcgctga tcaaacaata cctgaacttc
    12721 tggtgtgtct gccaaaggat agatcaattt atcatattct gctttcatca cactcggaag
    12781 catcgtttgt agacaagtga ttttgaatgc gtaagtaatt tttttcatat aatcagctaa
    12841 tgaaagtaat tcttcattga ccaccggagc aaggtctagc aaacgaataa ctgccttcaa
    12901 agatggatta gctgattctt ctgatttttg gatagccata acaaatcctt ggacatgacg
    12961 attgccatta ccaaaaggga cttctactcg catcccgact tgcaaagctg tttccatttc
    13021 agagggaacc aaataagtga atggctgatc ggtctgcata gtaggtacgt ccacaatgac
    13081 ttcagctgtt aatgtcatgg ttcgtttcct cctttttttg tcttttctta tcaagcgaca
    13141 aaaagttcct tgcctatttt actagagatc gatggatttg tcttatcttc tgccaaatcc
    13201 taacaaaaaa ttaacagcag ataagaaaaa aaagaactgt gtcaaaaagt tcagacacag
    13261 ccttttctac cagacaataa gtggcgaatg tttgaaaagg aaacagctgg gtttgctttt
    13321 ctttttagat gttcgctcta tttttcttta ttacattttt tgctcttcac ggatacgtga
    13381 ttccaattcg tgttgttcac gctctttttg tgcttgacgt tcctcttctt ccatcttcaa
    13441 acgttcacgt tttaattcag gatgtggatc attgactaca tttcctgcat cgatttcttc
    13501 taaagcttga ccgacgcttt ttactgaatc aaaagaatct agagtaggct gtgcacttgc
    13561 atccaattca tgcgcgcgtt tacttgctaa aatcactaga gaatattttg aatttacacg
    13621 atctaatagt gagtcaattg aaggttttaa catcataaga ctacatctcc tttaacattt
    13681 taatatattt accgattacg cgatctactc gaaaatgttc actggcaata atgtttttga
    13741 ttcgttcaac tgctagcggc acttcatcat ttacaacagc atagtcgtaa agagccatca
    13801 tttcgatttc ttcttttgca actttcattc gtttttcgat tacttcatct gcatctgttc
    13861 ctcggcctac gatccgtgat tttaattccg ctaaatctgg cggagtcaaa aagatgaata
    13921 caccatcggg cactttttct ttgacttgtt ttgctccttg gacttcaatc tccaagaaaa
    13981 catcttttcc ttcatccaat gtcttgttga catatgacaa aggagttcca taataattcc
    14041 cgacatattc tgcgtattcc agcatctctc cagcttcgat cattgattca aactcttctt
    14101 tgcttcggaa gaaataatca acgccttcga cctccccttc acgcatttta cgggtagtca
    14161 ttgagatcga atactgaaaa tcattatcct cactctcaaa aattgctttt cgtactgtgc
    14221 cttttcctac accggatgga ccagacaaca cgattaataa tccacgctct gacatgacga
    14281 cgtcctttca actagtcttt ttgtacttat cgtttatttt gcactttttt tctgcaactt
    14341 tcaaggttat ctttaattat agctagaaat tggtaaaaaa gctaccctgt accaagaggt
    14401 cgtaaaaaag ctgtcctgca tcaagtaata agaacagcca aacataaaca gcttctcatg
    14461 tttttcgttt ggttggggtt attatcgcag atgcaagctt ttgaacgccg tgtataagag
    14521 gtcgtgaaga agccgattag tttcgagtat gaagaaaaat tttgtgaaaa tagtttctca
    14581 catttttgca taaatttggc ttaataccga gaaaatggtt tttgaacatc acttattcaa
    14641 aataaaaaaa gattgtgaca cgaattttgt cacaaccttt aaatgtattc ctagagcaaa
    14701 gaagctaagc gaagaaatcc aattctgttt atttattgta tttctttgtt cagcatcctt
    14761 gctaaaagcc catattggac aaagtattca ctacacccat gatctcatct tttcgagaac
    14821 gtgctttttc tgtcggatct tctacctctt ctgtgtcaat tcgaacaata aatttccgat
    14881 tcatcgtcac gtaagcaccg tcgttgatca aaataaacag ttcgttctca ttatagtctg
    14941 cacaggcatc tttccatacg tcaaagccgt ttttttcaga agggatatcc cgtttaacca
    15001 cagtttttcc acttaattcg tggaacgtta cgtcataata tttcattatc tctacctctt
    15061 tcgtcattca atattttgta cttgttctct gattttttct aagatcgtct tagactggac
    15121 gacttgattt ttaatttcga ttgcatttga tttggaaccg atcgtgttga tttcccgatt
    15181 catttcttgg atcaggaaat ctaattcccg acctacagga cattgtgctt cgataagttc
    15241 atctagtttt tgtacatgga tcgccagacg atccagttcc tcgtgaatat ctccgcgttc
    15301 caacaagatg gctagctccg tcaacaaccg ttgctcatcg actttcgctc caagaaattc
    15361 ctccagcttt ttttgatacc ttatttgaaa ttccttttca tagatttcta caaaactatc
    15421 gagttttttt agtacttctt tgaattcttc agtattcttt ttcaacacta aagctaaagc
    15481 ttgaccttct ttttctcgac tcgcctcaat agaatcaagt gcgcttttca atgtatctag
    15541 agctaactgt gccaattctt ccatgctctc ttccggtttt tcttctaccg ttacgaagtt
    15601 ttcgtccagc acgaagcgtt ccgccatccg actaattgac acttgatttt gactaccgta
    15661 tcgttgttga attccgtctg tcaattgagt gataagtgaa tcaatcaatt cccagttgat
    15721 gacgatctgt ttttcgtttt tgccaatata agctagattc acaaatactt cgattcgacc
    15781 gcggcttaac tgctgtttaa taagctgacg aaaatcgttt tcaacaaagt tcaggatttt
    15841 aggacttcgt atttgaacat ctagaaaacg ctggttgaca ctttttattt caatatctaa
    15901 ctgatacgct tttgtttcgc ggatggcttt gccaaaacca gtcatacttt tcattcgaca
    15961 gggtccttcc tcattacata ttttctattg ctttttgcag ttgttcaaac tcttcgttcg
    16021 tcaatgtcag gcctttcccc attttttcat ggtctggcgc ccaatctcga agatcgaatt
    16081 ttggcgggcg accattccag ctgactaggt tcagttcttt acgccacccc ttgttgtttt
    16141 ctgacaatat agcgatttct tctacgattt catatgaaaa ttcttgtgcc atggttgttc
    16201 ctccttgaga taatataaat ataggatcag taatgtgtca actggttgct taagcaattc
    16261 atgctgcaaa cgatccttgc gttttccttg tagcagataa tcaaaataaa cagttttcgt
    16321 gcgctcattt tgtatttttt gctggtattt tcgatacctt cgccattctt cagtacttaa
    16381 ttgaccaatt tgtgccaata aatcatccgg catacttttt atatcttgtt ggcagatcat
    16441 ttgaatcatc atgtctactt gatagatttc attgggcagt aaaaaatgtt tttttgctaa
    16501 aagttctagc atcgccaatc cgcatgtctc tggtacgacc ctgttcttat ataaattacg
    16561 cagtttttgc caaaatttta tgcttttgaa aaaagataat tctaactgaa tttcttttgt
    16621 taagtaagac aaaaatcctc gccagtatgt tcttgcaggt ttaacagaat gaaatgtata
    16681 ccagttccca tagtaatagc ctaatcgttt tttcatttcc agatagccta attgtaaatt
    16741 taattggttc ctttggctat caaaaagcag aaagctgcct agtgtccaca atgtttgaca
    16801 tttccaatgg ataaaaatgt ctggcatacg aatttttttc gctgggcctg gcccttgcac
    16861 atctacgaca aatgcctctg ttgctccttt gttaatcgca atatcaatag gtattttgtt
    16921 gcgataccca ccgtctgcat atttattttt tccgatcttt cgataagcca tagcgggata
    16981 aaatgaagca gaggcgagta tccaatctgg tatttcttct gtttttgtct gttggatatc
    17041 agtcactact tccgctagtt ctggcagttt agtagacacc gtatataaac gtattttggg
    17101 attatgtttg agtaaggaca aatccaaatt gtctttgacc atcttttcca atggagcaga
    17161 agaaaccccg ccttcaatta gtgctgtttt cgtcatttgg cgtctttctc gatagaagcg
    17221 ttcacgaagg tcttcaactg cagccatttc tggtaacgca agcacttgtc ttgtcgacag
    17281 ctttttccat aaattcacag cttttttcat atctccttgt aaaatcaatg cgccgttcaa
    17341 cgcacctacc gaggtacctg tgataatctc aaagttgata tcatgttcct tcagcgcctg
    17401 ccacacacca atctgataag caccgtgcgc tcctcctcca cctaaaacaa gtgctgtatg
    17461 atagaccagc tttttattta attgtgtggt tccttgatag ccgtttttat gaaaaatacg
    17521 caaccattct tctgatagag gagattgaaa taaaaaacaa cagttttctt taataaaaaa
    17581 acgagcgcaa gcttccatat accgaaaaaa ttttttccat tcaaaagttt caccttcaat
    17641 caacagatta ttaaaaataa catctcctct acttttagca atagtaaaat agacggtata
    17701 ttttttcgat actgctttaa aagcacattg cttttttgca gaacgccaaa aagcgtctct
    17761 actttcttga tgactttgaa aaaatggaag gggttgtttt gcttgctgga taggcgaata
    17821 cagcgacatt ttctgtgaaa agtaaacttt ttttatctcc atcgcgctct cctttttctt
    17881 caagtatacc agtacgtcat aaaaaggtca tgagtctcgt attcgacttt gtagattcca
    17941 taagaaaaca attcataaaa cctcctttaa ataaaaaaat acaaaaatca caaaaattaa
    18001 ttgaaaatga gagtgttttc aatgaaaacg tgattttgta atggaatttt aaaataaaag
    18061 aaacaagctt ttttaatgca aatgttgtaa aataaaagag attaagagga aataagagga
    18121 ggaacatgat gactgagtca gtttatgtac atattgatac cacaagtaat gctgttttaa
    18181 caaaagggct gactgctact gactttgcaa acagtatcgt gcattttcct caaaacttgc
    18241 tcttgcttga tccatctgct tctgctggag aatatgaatc ccatacaggg ctgaaagtaa
    18301 tccgtggtac agaaaatatt cagcgtttct tttcctcttc tcgtaacagg cgtggattcg
    18361 atgaattgaa gtggatcgac tttactgatt taacgatgct gaaagaattg acacctttag
    18421 aaatttcaga attattatat tttggtcata tgaaaacaca tcttcattcg ccgttttttt
    18481 ataaactaca gaataatttt gtatacttcg atcttaatga tcagctgaat cggatttatt
    18541 atcgctattt agatgaattt tataggattt ttgctagtaa attaagttcg atcttgtcag
    18601 ataagatcaa tgtaaaaaaa tcttttttca aaaaagaaac acctgttgaa aaaatcagtg
    18661 tggaattatt gaaagaaatg cgtaatttga tgcaggaagg gatcattttt tcttttgatc
    18721 aagttggttt gatcgatggt aaatatcgta tcccaatcta tgtgattgaa gattatattt
    18781 ggaaattaaa aggcacccgg tatcgagaag aggaaatcat cgctacttta atctactctg
    18841 tcaccgatcg acactggcaa ttggtagaag atgccgatct ttcttatctg ccattgaagt
    18901 agatctattt cttatcaaaa aataaaaagg aaacaagaaa ctaggacatg attctgtctc
    18961 aagtttcttg cttctttttt atttacagct cctgtcgttt atctggcaca aaaatcgaag
    19021 gagtcgtttg ttggatccct aataaaacga tgatcgttac aatagctgta ctcaacccgc
    19081 tggcaccatt cattacgaaa gaaaagagcc aaggattcat cccccatagt gcatacgctc
    19141 cccagaaaac cactcctgca ataaaatgcc aaaaatatct ggaaagtgcg ccaatcaatg
    19201 caccaaacac gatgcttttt ctaatttgtt tcgtattatc gtctacatag cctttttgga
    19261 catttgaagc catgagccct gctgcgcctg caaaagtata ggcaatcggg tactcaatca
    19321 acacttgaac gacacttaaa taataaactt gtcccaacgg aaaatgtagg atgccccaaa
    19381 tgaaacctgc cagcatccct gcctttgtcc ctcttcttaa cgcaaaaaga gtcaatggaa
    19441 tctgccctaa tgaaatagaa aaactgctgc cgatctggat cggaatcaac gacaacacca
    19501 tcgctaacgc tgcaacaatc gttccttcta cccaaactct attttttccc ataaaaaaac
    19561 ttcctccttt gaataaaaaa gtcacaaagg aggaagcagc tttcgaaaat agtcaaacag
    19621 ctgtttattc acaattccta cgctcgcata accgatacag gtacgaaggg tttagaaata
    19681 gattcaatct cagcccaatt ggactcccct ttgtgagttt atttattatt tattttttgt
    19741 ttcaaacaat ggactaactg gtttgttttc atggatacgt ttgattgcat ctcccattag
    19801 tccgccaaca cttacttcat cgattttgtc gattttacga tcttctggaa gatagatcga
    19861 gtctgtaaca actagacgct cgatagctga atcttctatt ctttgtaaag cagggccgga
    19921 taatactgga tgtgtacatg aagcatacac actagttgca ccagcttctt tcaaagcatt
    19981 ggcagccaat gtgatcgtac ctgctgtatc gatcatatca tcgatcagta cacaagtttt
    20041 gcctttgact tgtccaatga tattcattac ttctgcaaca tttgcttttg gacggcgttt
    20101 gtctatgatc gcaatcggag atttcaaaaa ttccgctaat ttacgtgccc gtgtgacacc
    20161 accatggtct ggtgaaacaa cgacaacatc atcgcctttg attccttttt ctaagaaata
    20221 attggcaata agtggcgcac ccatcaaatg atcgactgga atatcaaaga aaccttggat
    20281 ttggactgca tgaaggtcca aagtcagcat tcttgttgca ccagcttttt gaatcatgtt
    20341 tgctactaat ttcgctgtga ttggttcccg tgcacgtgct ttacggtctt gacgcgcata
    20401 gccataataa ggcatgacta cgttgattgt ttttgcactt gctcgtttca gtgcgtcaat
    20461 catgatcagc aactccatca agttatcatt gactgggctg ctcgttgatt gaataacata
    20521 gacatgcgat ccacgaatac tttcttcaat gtttacttga atttctccat cactaaattg
    20581 agtaacagat gattttccta actcgacgcc gactgctgct gcaattttct ctgctaatgg
    20641 gcgattggaa tttaacgcaa aaatttttaa tcttgggtca aagtaatgtt ttgacatggg
    20701 gacctccact ttctttttca caactctaga attctaccta ataaatagta gtcatatttt
    20761 ttaaataaat caagtgattt tgcaaaagtc ttagtttaga taagggagtt tttttgcata
    20821 tccttcttta ttgacttgtc ttgctctcgc aattgccagc gcatattctg gaatattgtc
    20881 tgtaattgtt gacccggcag caatcgaact gtttttagct acctcgacag gtccgatgat
    20941 attcgtgcta gaaccaataa agctgtggtc gccgacagtc gtatgatgtt tgttctttcc
    21001 atcatagttg acgaagacaa caccacatcc gacattgatt tcttctccca gcgtagcatc
    21061 accaacataa gtcaagtgac cgactttagt tcctttgccg atttgagcat ttttcacttc
    21121 cacaaagttt ccgatatgga cgttttcgcc aatttcagct tttggtcgta aatgtgcata
    21181 aggtcccaca tctgcatgtt tcttgacatg gctgctttca atgactgagt tttcaatgac
    21241 gacgtgatcc tcaatcctac tgtctacgat ttttgaatgt gacccgatga cacaatcaga
    21301 accaatgact gtatttcctt ggatttgtac acctgcttcg atcaatgtgt ctggaccgat
    21361 ttctacgcct gcatcgatgt atgtcgttgc tgaatcgaca aagcttacgc cattgaccat
    21421 atgcatctgg ttaattcgct ttctcatgat ctcatttgct ttagctaacg cgatccgatc
    21481 attgactccc attgattctt caaaatcatc cgtttgataa gcagcaactg tatggccttc
    21541 ttcttttaag atttcgatga tatctgttag atagtattca ccttgagcat tgtttgttcc
    21601 aactttgctt aacgcatcga acaaagcttc gttgtcaaaa cagtaagttc ctgtattgat
    21661 ttcttgtaca agagcttctt ctggtgttgc atctttttgt tccacgattt tttctacgat
    21721 cccaatatgg tcacggatga ttcgtccata tcctgttggg ttttctgctt gtgccgtcaa
    21781 gatcgttgca cttgcatttt ttccttggtg atattcaaaa agattgttca gcgtttcagt
    21841 agtcaataat ggtgtatcgc cactgataac taacgttgtt cctttttttc cttccaagaa
    21901 agaagcagct tgcaaaacag catgtcctgt tcccaattgt tctgcttgta acgcatattt
    21961 actgcgttct cctagttgtg ctttaacttg ttctgctccg tgtccaacga tcgtaatcac
    22021 ttcatctggt ttcgtttcac tgacccgatt aatgatatgt tctaccatag gtttaccaga
    22081 aacgggatgt aaaactttat ataattttga cttcatgcgg gtacctttcc ctgccgccaa
    22141 aataattgca taacgtgcgt ccaactgcgt cactcctaaa tatagtttgc tgatttcagt
    22201 ttatcccagt atacccattt tttcaacaaa acaagtgatt ttcttttatc ccagcgtttt
    22261 ctggataaaa aaatagatag accataacgg gcctatctat ttggtaaccg gtgctctcct
    22321 gccaactcct cggtcacaag tcacactact cataaaacat gaaaagctgt tttttgaaat
    22381 cgtttcttgt gacttagagt taaacggtag gcttacaacc tcttcattaa acgtttttca
    22441 atcagctgac cttcggtatt agctcaataa acggcaaaat atgaggagca atttttccgt
    22501 ttattgagct aatactcagg acaggacgct gatttcacaa cctctagata aacggtgttc
    22561 aaaagccagt ttctcggtat taagccaaat ttatgaaaaa atgtggaaag ccattttcac
    22621 aaaatttttc ttactactcg aaactaagcg gctttttcac aacctcttat tctgaaaaat
    22681 aatttcctgg cacaaccgta atatttttcg tttgagtatc tacatctttg acataaagca
    22741 gtgacgtata atcatcgatg gcgcgatgac cgttgaattt tgattcggca aatacagtga
    22801 tgccaacaag ttctgattcg aattcttcaa tcaagctctt cattccgttg actgttccgc
    22861 cgcctttcat gaaatcatcc actacaagga cacgtgaacc acgtttcaaa cttctcttag
    22921 acagttccat cttttcaatt ctttcagaag atccagacac atagttgaca cttactgttg
    22981 atccttctgt tatttttgag tcccggcgta caataacgaa tggcgcattc agataatagg
    23041 acacagcttg ggcaataggt acgccttttg tggctacagt cattacagca tcgatttttt
    23101 caccaaggta tttagaagca atgattcttc caacttgacg cagcagttca ggttctccta
    23161 acaaatcaga gagataaaca tatcctcctg gaagcagacg gtcttgttct gatagacgtt
    23221 ctgctagaga ttcaatatat tttttcgcct cttcatatga aatttcaggg atgaacaata
    23281 caccacctgc agcacccggg attgtttcca aggttccata ccctcgttct ttaaatgttt
    23341 tcttgatgat tcccaaatct tcactgatag aagatttagc agattcataa cgttcggcaa
    23401 agcttgttaa ggaaatcaat tcatgcgggt gattcaataa atactgtgtc atgtcaacta
    23461 gacgttcact gcgacgtact ttcacctatt ccaactcctt ttaagaaatt ttatcgtttt
    23521 acattatata agtttttctt caaaaattcg agcattttat gaaaaaataa gaaaatagtt
    23581 cggtatttat taaaataaaa agaagcctat aaaaaatttt tcgtttttat aaggcttctt
    23641 ttctttataa tttagttatt ttttcttctg aaggcaacca atattcgttt gatgatattt
    23701 acaatcagga acaaagcaat aaagactaat gtgattgttg ctcctggagg tgtatctaga
    23761 taaaacgagc tagttagtcc tgacagcatc cctagaaatc ctacacaaac actgatagca
    23821 atcacgacat tgaagctttt gcctaatcgc ataccgattg ctgcgggaag aaccatgatt
    23881 gcggatatca aaagtgcacc ggcaatcggg atcatcacag caatagccac accagtaatc
    23941 acattgaaca tcatcgacat caaatgaacg ggtagcccat ccacgtgggc ggtatcttca
    24001 tcaaaagtca gaacatacat cggccgtttg aataaaaaga acaatatacc gatggcaacg
    24061 aataagattc ctaaaaagat gacttgctcc catgtaatgg tcacgatcga accaaacaga
    24121 taggattgga tacttgttgc tgaattccct ccgctcaaat tcatcaatac taacgctagc
    24181 gccaaaccac cagccattaa aatggcaatg gagatttctg aataggttcg gtagatcgtt
    24241 cgcagatact ccaatatcac agctgctaat acaacgacta gcatggtcgt aagtgtagga
    24301 ttaaggttca aaaagaatcc taacgctacc cctgctaaag agacatgaga aagcgtatca
    24361 gccatcagcg attgtcttct gatcactaaa aacacaccta gcatcggggc gattccggca
    24421 atgaagatag cggcaagaaa cgctcttctc atgaattcat atgaaagcaa cgccattgag
    24481 aatcctcctt tctcaccagt cggatctgtc tgtccgcata ctgtttgata tcttcatgat
    24541 catgggtgat cattagaact gatttcccat ggacatgcgc actatgtcgg agaagacgat
    24601 aaaattcatt tctggattct tcatccatac ctgttgttgg ttcatcaaga acaaataagt
    24661 cagggtcagt agcaaaaata cgcgctaaac tgattcgctg cttttgtccg ccagacaatt
    24721 cacctattcg ccgatttcgc atatcccaca tccctacagc ttctaaagca cgctgtacat
    24781 gttgatgatc tttttcagag agacgcttga accagcggcc tcttggatat cttcctgaac
    24841 ggactaattc gatcaccgta ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa
    24901 tataaccgat acttaatttt tttccttctt gatttttctc agcaatcttt atcattcctt
    24961 ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt tgacttggct gctccatttt
    25021 ctccagttaa aatgacaaat tcacccgcat ctacatgata agagacatct tctagtacag
    25081 gttcatcatc atagtaaaag gtcaaatcag atatttcgat ataacgcacc ttacaacctc
    25141 cattctaaaa cttaattgat actttctttt aaagctttca gatttttttc catgacagaa
    25201 atataatttt cgcctgcttt gatttgttca ttagtcaagc tttctaaagg attcaacact
    25261 tctagcttca ctcctgtttc attcgacaat gtctcagcta ccttcgaaga ggcattttct
    25321 tcaaaataaa tcactttgat gttgtttttc ttcacaaatt cttttagctc agctaagcga
    25381 cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat
    25441 tgattcgcta aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct
    25501 tttagctcat cggagtattt ttgatccagt gcttcaagct ttttgatata agcatcggca
    25561 ttcttcgtaa aagtttcctt tttatctgga tatttttcgc ctaattcttc agcaatcgtt
    25621 ctgacttctt ttatagctaa taccggatcg agccaaacat gtggatcgta ttcatgtgaa
    25681 tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg
    25741 attcctttag ctgcttcgat catcaaggtc tgtttgctat ccactgaatc ctttaacgaa
    25801 tccacaaaaa attccatgtc tgaactatta tacacgaaaa catctgcatc ggaaatttct
    25861 gctctttctt ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt
    25921 ttcacgtttc cttcatcccc tacgatctct tttgtaaaat catacatcgg ataaaaagtc
    25981 gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc acatgctgca
    26041 aacagtaaag cacctatcat taccgtagct gtcaaaagat attttttcat ttatatgctc
    26101 ctttcaatga acaaatcgga attttacgat ttattaacgt gaactaatgt atcaaaataa
    26161 aaaaacacag tcaatagaaa tccatcaatt tctgatttaa gcatgtatat tcttgattaa
    26221 atcgtaaaat ggatgagttg ctgctcatat ctagccattt cactacaata aaaaactgaa
    26281 gaataccatt ttaggattcc tcagtttttc atcacctatt ttacttctta tattttattg
    26341 cagggttcgt actaaataaa cttcctcaca aaaacctttt aacccattat atactctctg
    26401 cgctcttgaa taatgttgac acaaggcaaa aacggttggt ccgcttccac tcatcaatgc
    26461 agcatctgct ccgtacttga tcatacgctc tttgatttga tggatgactg gatgccgctt
    26521 tcccgttata tcttctaaac tgtttcccat ttttttgatc atctgctgat aatcttgctc
    26581 tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg tatctacttc
    26641 tctaaacact gtacgtgtag agacactcat tcttggtttg accaacacaa cccagcactg
    26701 tggcatagaa ggtaatactt ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc
    26761 gtagacacag taaggaacat ctgtaccgac ttccaaaccg atttctgcca gttcttctaa
    26821 cgaacagccc agattccata attggttgat ccctcttaga gcagctgcac agtctgtact
    26881 tcctccgcct aatccagctg ctactggaat attcttctta acaaagatct tgacaccggt
    26941 tttgatattg tatttatttt ttagcaataa agcagcttga taaacattgt tccgttcatc
    27001 taccggtaaa aatgctttat ttgtttctac aatgattttg tcttctgtta tttcttcaaa
    27061 aaatagatga tctgccaagt ctacgctcga catcaccatt tccaactcat gatacccatc
    27121 ttgccgtcta tataatacat ccagtcctaa atttatcttg gctggcgctt tttcaatcaa
    27181 ttccatcctc tcacccattc gaaaagtcta agtcgcaagt attctatcat agggaaaaat
    27241 aaaagtatag cttatgtctg cctagtggtt gattttgtca tcgtttcaac agctttcaca
    27301 gaattttctc ttcataatcc aaacaagttc tagtgattct tataattctg tgagcacttc
    27361 ttctgccaaa ctcggaacat ttcctttttc ttttcctatc agactgagta gctgcatggt
    27421 agcaaatccg aaattacttc ctttcctata agcaaaataa cgtccttccc aatgatcgac
    27481 atattttaat ttcttttcat aagcagactg gaaggaatag aaaaaattac cataattata
    27541 tacgatatgc atgacttttt cttgaaagaa agaaaaatct gtttctccaa cattagctaa
    27601 tggagctaag cctagattgg cttccgagta gcccattttc tcgtatacaa taaacagatt
    27661 ggctaacaga tagtcagcta attcctcaga ttctttaggt aagacccgca acaaatcata
    27721 tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt tttgatcttt
    27781 acggacaata ccgacaccgc tagataatac atatgattta tcaaatcgtc ccacagagaa
    27841 gtatttttct cgtgcacctt gaagccattt ttgtgaaact tcatttacag cttgatgtaa
    27901 ttcttccggc agttgatcgt accaagtaaa atggtatcct tctttcatca aacgttgata
    27961 ttcaagttgc tgactcagcc gagacaagtc ttctgttgct agatcaacaa tccctgcttc
    28021 acctactttc gtgaagtgaa aacccaattc atgaagcatc actacgtagc tttctgatat
    28081 cttataaaac gcaagctgat agcctaaaca atctgcttgt ttcataaact gattcgttgc
    28141 tgcccgcatc attttaggat tgccaactgg atctcctaaa acaaagcatt tgtttccttt
    28201 gatctgataa ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa aataactgta
    28261 acctgacaag cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc
    28321 aaaacggtct ttttcgtact cactgccaag cttcgtagta tcttcagcta aatattgata
    28381 aagcgccact aaagctagca atgagatgcc caaaccaata agccctgaga accacacatc
    28441 atctgaagga aacaaaataa aatgatttcc tgttattcga ccgactaaaa agcgtccgct
    28501 gtgatagcct gcaattgcgt aaacgataaa caaaaagccg aacaagctag catcaaagat
    28561 tatagctccc caagaataaa cgaattttgt tcgataaaat tcttttctgg ataaccacac
    28621 agctaacaga atcaagacat ataccaatat caactgccaa gataacgtac gtgtgattgt
    28681 atttaaaata ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt tgacttttac
    28741 tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc aatagattca ctgtctgatc
    28801 taaaaaattg aatgagaacg gcaataatac ttgaaatagc cgactgattg cagacagatt
    28861 agtgattgtt gatagaagta ccatcatgat ccccgcaaaa tagacagcac ttacgagcac
    28921 gatatgcgca accttttgga aaaacagtcg cggaatgtta tctaaaaatc gattgatttt
    28981 gactcctgta tgtgtgatga acaatagaag tcccgtcata aaaggtagaa cataataaaa
    29041 caaccgataa tataatagcc aaactactgc tacatcttgc cgaacagaca attgactcat
    29101 tcctaagatc atcaaaacgt caaaagtccc cattccacct gggaccatcg tcagcattcc
    29161 tatcaatgta gcaatcatga acataggata gatcgaaagc atcgaaatat ctattctcat
    29221 caacttgccg atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa aggatgccag
    29281 tagcagacca agcaagcctt ttggataaaa ctcgaagaac aatgtctttc tctttagata
    29341 agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg aaaagccaga tccaatagct
    29401 tcgaaaaata ctttctgact gaataaaaaa gacatcaata aatgtaagaa acgaccatac
    29461 agacaaacca gtcagcataa atagtgcgac ttttgataca gtggctaaaa ccatttttct
    29521 atttgtctgc tttccataaa aactagcccg cagacttgct cctaccacac caccaaaacc
    29581 ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt ttctttccat
    29641 ttttggtttt ccttgttttt ctaagactct gatcgtcacc caatcataca acaacatcgg
    29701 agtgacaccc agcagtccag ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc
    29761 catcctttgc caaatatctt gccaactcat gccattagca atatttgcga cttgatttgc
    29821 cacaaagact aacacggagc cgaaaaaaat cagttttaaa atcagactat gttcctttat
    29881 ccattgcagc atcaagtatc gtttattttt cacattttcc cattcctaac ttgtatactt
    29941 ttgtgtcata ccccaatgtt atcttattta ttttaatttt tccactttag acctgttctt
    30001 tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa aggagaggat aacatgaaag
    30061 taggatttat cggcacaggg aatatggcac aagctattat tgatggttta ttaaaaaaag
    30121 gaacagtctc gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat
    30181 ttgctgctga aactggcgtc catgcatgca aaagtaatca ggaagttgca tcacaatcag
    30241 atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa gaactaagag
    30301 aatatagcca gaaagtctta ttcatttcta ttgcttcagg aaaatcatta atagatttag
    30361 aaacagcgct tgaagatcct caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca
    30421 gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc tgccacggag gaacagcttc
    30481 agcaagctac gatgattttt gaagcaatcg gcagcgtcta tcctttagca gaatcagatt
    30541 tcagtacatt cattggcctt gctggcagct cacccgcatt cgtttacatg ctgatcgatg
    30601 cgatgggacg gactggtgta ctgaatggtt tgtccaaaca gacagccact gagattgctg
    30661 caaaagcaat tatgggttct tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac
    30721 ttgtagacca agtttcttct cctggcggta caactgttgc aggtgtagtt gccttagaag
    30781 aagcaggatt catcccagca atcatcaaag gaatcaatac gacgatcgag aaagatcttg
    30841 agatgttgaa taaaaaataa tgatagcaat gttttttcgt gccttacaaa aaaagaagag
    30901 ctgtggcttt tgccgcggct cttcttttgt attttttatt atttagcata atcagttgca
    30961 cgagtttccc gaataacgat tactttgatg tgtccaggat aatccagttc ttcttcgatt
    31021 cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat ccgagatttc ttctggcttg
    31081 accattaccc gaacttcgcg tccagcttga actgcgtaac ttgattctac accggcgaag
    31141 ctgttcgaaa tattttccag acttcttaaa cgacggatgt agttttctaa tgattcactt
    31201 cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag ctacaaggac agagataaca
    31261 gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt tgattactac tgggttttct
    31321 ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag atccttcaat ctcatggtca
    31381 agtgctttcc caatatcatg gagcaacccg gcacgttttg ctaattggat atcttcgcca
    31441 agctctgctg ctaagatacc agctaatttt gcaacctcga ccgaatgctt caagacgttt
    31501 tgtccataac tagttctgaa tcgcagacgt ccgatgatct tgattagatc tggatgaagt
    31561 gtatgagcac cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg ttcatccatt
    31621 tctttacgag atttctcgac catttcttcg atacgcgctg gatggattcg tccatcttgt
    31681 atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga
    31741 acaactgctt ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt
    31801 cgaatattac gaccttctcg tccaatgata cgacctttca tttcatcatt cggaagggta
    31861 acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat cgctaatgat
    31921 aataagtttt tcgctttgcg atctgcttct tctttggcac gctgttcaga ttcttttacc
    31981 attagagtta actcatgatt cagctcttct tcagtcgatt tcatgatgac atctcgtgcg
    32041 tcctcttttg ataaaccagc aattttttct agttcttttt gttgatcttc aatcattttt
    32101 tcaacgtctt tttctcgctc ttcaattaat tgctgttttg aactaagtgc ttcttcttta
    32161 ctttcaagtg agttctcgcg tttttctaac gagtcatctt tacgattcag cgtttgttcg
    32221 cgttgaatca aacgattctc ttgagatttt aattctaact tactttctct cagctcactt
    32281 tcaacttcag aacggtattt ctgattttct tccttagctt ctaacaatgc ttcttttttt
    32341 agagtttctg cttctttttt tgcactatcg atgattccag cagctgatga ttgtgcacca
    32401 gctatttctt tctcgtgacg tgactttgcg accataaacc ctaaaccaag accgacaatt
    32461 aaaccgatga tagcgaggag aatgttgaat accataaaat ccacctccat actatctttt
    32521 tcatttttat catctataca gcttctatga taaattattc taaactcgtc aatatgcatc
    32581 ttcgcatata tgtgtacaaa aaacatgcac aactttattc taatgtttgt accccgtagt
    32641 gtcaacttta aataaacaaa gaaacggctt tctacttatt cattaaacga tggtttttaa
    32701 aataaatgac actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat
    32761 tattctttgt agatgaacag atatttttat tttatgtacc cactcgtata attaaataac
    32821 tcacgatttc tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac
    32881 cagccgtttt tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt
    32941 tttcatcttc tgggacagcg acttcttcac caataccata agctgctcta actagagcag
    33001 aaacttctgc catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt
    33061 gaccaatccg atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt
    33121 ctactgccat gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta
    33181 cttcagcgat tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt
    33241 taccaacaat atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg
    33301 ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc tggattccca aacatcacgc
    33361 ctactttttc acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac
    33421 cagagagttt acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac
    33481 ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga
    33541 tatctacagc cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt
    33601 caggctgtga aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat
    33661 ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa
    33721 tagcgtgtag tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac
    33781 gtccacgggg gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa
    33841 ttgtagagat ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc
    33901 tcttttcaat ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga
    33961 taatcctcct tatatttaca aatcagagca acatttcatt atccttatca tatctttttt
    34021 agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct
    34081 gttttctcaa aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc
    34141 tggcaaagtg atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg
    34201 ttcctgcaga ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt
    34261 aatctgttct tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga
    34321 ctgttccata ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga
    34381 gcgagtaggt aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg
    34441 cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt
    34501 tcagtagttc cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt
    34561 cccctactcg ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa
    34621 catcatttgt tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga
    34681 tcgttggatt gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac
    34741 caatcccata aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct
    34801 ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa
    34861 gaaggatata cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat
    34921 ttggtagagg aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt
    34981 ttcgatgggt tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga
    35041 gagacttacc gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta
    35101 atccgccaca taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta
    35161 aacgctgctg attatctcct acaactgttt gataataaac ttcgatacct agatcagcta
    35221 gctgttctga caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc
    35281 cgacagctat tatttctgct ttcattactt cccgctccct tatatagtat acgctgtatt
    35341 tttatatttt tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc
    35401 cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat
    35461 aaaacatgaa aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct
    35521 cacaacctct ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat
    35581 gaagtaatcc acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc
    35641 tactggaata ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac
    35701 catttgtgta gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc
    35761 gacaagtagt aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac
    35821 ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt
    35881 atctgccaac gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat
    35941 ttttccatct aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg
    36001 tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac
    36061 aaccaaaatg aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt
    36121 cacactatcg actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata
    36181 ttatttactt ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt
    36241 gaattaacga agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca
    36301 agtaaacaga tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca
    36361 cattttttgg actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt
    36421 tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta
    36481 acacggtact ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta
    36541 ctagcacacc taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag
    36601 cgtctgtgat atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt
    36661 tcttttcttc acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc
    36721 tttgctctga ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt
    36781 ctccgattat tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa
    36841 tcgccaaagc aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat
    36901 gcgattcttc ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt
    36961 cagggaccga tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat
    37021 cttctcctac agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca
    37081 attggtcaaa atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct
    37141 gctgtaattc atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga
    37201 tggtttcaaa agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct
    37261 gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt
    37321 ttccgaataa acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa
    37381 tataagaagc tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg
    37441 acctctttct taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa
    37501 aatataagga gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca
    37561 caacctcttc cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt
    37621 taaaaacatt aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    37681 tttcacaacc tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat
    37741 atcacaaaaa tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac
    37801 ttctgtcaca acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa
    37861 gagagcatga tttgttttat tttctcacta atttttccaa cacaactaag aataccataa
    37921 aagctgattt tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc
    37981 ataaaatact ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa
    38041 atgcttcccc agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat
    38101 caaataaagt tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg
    38161 aatttaatga ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt
    38221 ctgacacttc cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt
    38281 tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga
    38341 attcaaaacc aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag
    38401 aagtattgcc taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt
    38461 ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg
    38521 taatggctgc ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac
    38581 gtacaatttc ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca
    38641 tcaatttctc aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa
    38701 acgtccgata gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc
    38761 ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca
    38821 tccgccagtt agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct
    38881 tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat
    38941 tcttttctac ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag
    39001 cattggcgga agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa
    39061 acaacttatg ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac
    39121 catagggaat aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct
    39181 tgtgatagtc atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat
    39241 aaagtgtttc attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa
    39301 aagaagattg cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg
    39361 ctctccatac gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc
    39421 aaaaaagaag cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc
    39481 agcattgctt ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct
    39541 aattgctcgt gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg
    39601 acactcatga atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct
    39661 ttctctctga cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag
    39721 accattagcg caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc
    39781 agctttgctt gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt
    39841 tgggtataaa acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact
    39901 gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt
    39961 gccatcattt cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg
    40021 actacgccca accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag
    40081 gcataggaag caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg
    40141 ttcttttcca agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat
    40201 aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca
    40261 ccattcacaa aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat
    40321 aatccaaagc ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgaa
    40381 taattcaaac tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct
    40441 tgttgcagac gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt
    40501 aagacatgaa gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca
    40561 cagttgattg cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg
    40621 cttgaaattt ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag
    40681 tatagcatag atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt
    40741 attttttatt tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa
    40801 agaattcaaa gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat
    40861 tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat
    40921 aggttggttt gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct
    40981 tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat
    41041 tacatttctg atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa
    41101 gaatttacgc tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta
    41161 tcttagtgac atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt
    41221 agagcaaaca aatctttccg atacttctaa ataataaaga atctaaaaaa aaagaaagcc
    41281 gattttcagt gtcggctttc tttttttaga ttcttaactt gctacttgtt cctttttcat
    41341 gtcgatatgt ggaatatcat cttccaaata cacatctgag attgccttga atccaaacga
    41401 ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg attggttttt ctggaaaatt
    41461 tttctggatc atgttaatcg tttcttgaac aatctttctt cctaaaccat ctttacggtt
    41521 gtcctgatga acaaggacac gtccaaaatg gatactctcc gcttcttcat atactcgcgt
    41581 gtaggcaagg atcttcccat cagtatcttg aaaaaacgta tgccaagctt ctccatcaat
    41641 gtcatcaact tcttgataag gacactgttg ttccacaaca aaaactgcga ttcgttcttt
    41701 gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat ttctttacct gatattccac
    41761 taagcaaacg ccccctgctt ttttattctt agcataccga ttcgcacaca aaaaagcaaa
    41821 cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa aaattcgatc
    41881 tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa agaattttct
    41941 tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac gccgcggcgt
    42001 tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc gatacgacct
    42061 tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc tactatcaac
    42121 tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaaagaa atgcaataaa
    42181 aataaataag aagctcatta agatttttct aaattattcc tcattataaa atttcaagtg
    42241 tttttttatt ttgagtttta ctctcccgta agcgctcatt acttgcttgt gatccattgc
    42301 taaacattca gccatgtcta caaaatcaac gccttgcata taataatgaa aaattttagc
    42361 ttctattttt gaaaactgga tttcgcaatt ttcgattgct tcttttaata aaagatgctg
    42421 aattgctaaa ggttcttgca atgtgatctc tgctaaaaag tctggtcctt ctgtctctat
    42481 tttttggtcg attgagactt catctttttg atcacgacgc ttcagcgcgt tctgcttcct
    42541 caacagagaa tagatttgat tgcttataac gcttttataa taacttccaa agctaacacc
    42601 tttgcttttt tcgtaagttc tcaatgcctg atataaagcg atccttgctt cttgttccca
    42661 atcctcagaa acataatttc ttaaataata ggtgtttttc aaacgtagta aaagtggaaa
    42721 atatttttga tacagggcag caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa
    42781 ctcctcagac ataaaataat tcctccataa attcgtttat ggaggaatct tatcatcaat
    42841 gattcatcga actgattaga acgatatgtt atttattttt tctcattaaa tcgtccaatt
    42901 tttgggacaa tttttttaac tgctcttcat tccatggaga attacgtcgg aaatcaataa
    42961 aatgcatatc tttattttct tgggctattg ttttttccgt tttttttatc gtcttatata
    43021 attcataagc agaagttcgt aatgctcctt gggaaaagat catccactgt tctgctaaat
    43081 cgcttgtcgc cactgtcact tgtgtcaaac ggtcattcag ctgaccagcg attctctcga
    43141 tataactgtc agccgtttcg tcttctttcg taaaaacaac atccaactgg tatttcttat
    43201 aattttgttg gatgcccgga actagttggg catcgaagac aacgatgact tccattcctt
    43261 catatttcgc ataatttgat aaacgatgta aaagagcttc tcttgcatcc gccattttgt
    43321 cctgcttttt aagctggaca agttccggcc acgaaccaat catgttgtag ccgtctacaa
    43381 tcaacagctg ttttttcatg gtctactccc cctgacgttg ggaaaaagcc tgatacataa
    43441 gcaaaccagc agcaacactg gcatttaagc tctggacgtg cccggtcatc gggatcgtca
    43501 gcaattcatc catttctttt ttcaatcctg gactcattcc tttgccttca ttaccaatga
    43561 ctaaagcaat tgatcctttg gcattccatt tttggaagtt tgtgccggtc atatctgtgc
    43621 caaataccca aaatcctgtt tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc
    43681 gtgcaacagg gacatgttcg actgccccag tggaagtctt tgttacgata ggtgtgattc
    43741 caactgctcg gtgttttggt atgatcacac catcgacgcc acttgcgtct gctgtccgta
    43801 aaatcgaccc aaaattgtgt ggatcttcga tgctatccaa aatcaaaaag aaggggtctt
    43861 cttttttctc ttttgttcgt tcaatcagtt ggtcaaggga taaatattca taaggtgtaa
    43921 tcgcaagaac gactccttga tgcacgccgt gatcgctcaa agtttctaat ttttgtttag
    43981 gaacccattt gactggcaca gcttgttcac gtgcgatttc tttcagttct tctacttttt
    44041 ctcctcgtga atcttcttga agaaagagtt tattccctcg cccttgttgt agcgcttcta
    44101 ctgcggcatg gtgcccaaag acgaaattat cttctaatgc ttcttcattt tgatcagaaa
    44161 ctgactgatt tcgtccagtt tgctttttat ttgaatatct attttgtttt ttgtcttcaa
    44221 atccacgttt cgattgatgt ttgtttgttc tttctttacc catttttttc accaactttc
    44281 tgaatacacc agcggatcaa ttcttccatc cgctcggttt gttttgtcaa atgcagatag
    44341 cccatgagcg cttcgaaccc tgttgcgacc cgatacgtcg taacatcagc attttttgcg
    44401 ctcgtatggc ttttagcatt tcttcctctg cgataataaa gttcttcttc ttctgtcaaa
    44461 agatgttctt ccagcatttg ctgaatcaaa aaggcttgtg cattcgcaga aacataattc
    44521 gttgccgcac gatgcagctt attgggtttt gtttctcctt gatcaatcag gtggtcacga
    44581 acatagattt cgtaaatcgc atctccaaca taagccaagg ctaaaccgtt caattgtgtg
    44641 taatctctca tgcttctctt ctccatcggg ttccttgcgg tgtatcttct aataagatcc
    44701 ctttttcttt caacaaatca cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg
    44761 cttgatttcg ttcttcgatc aattgttcaa tctcttcatc taataactct tctgctgtaa
    44821 ataaaatacc gaagatcgaa agccattgcg agaataaagc ttctccttgt gccaagacag
    44881 cggcagaaac tttcggctgg tctagataag tgttcagcca cttagcaagc tcatagacca
    44941 ctgtgatccc atttgctgca ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta
    45001 aattttctaa caaagcgatt ttatccgcat catctgccaa attctctgag gcagtttctt
    45061 ggcggaattt caaattgtca aacgtattct tcagtcgttg taaattggtg gctgcttctt
    45121 tcatcgtcgt ttcactgtag cggatcggac gacggtattg tgtggtcgcc atgaagaaac
    45181 gcaagacttg tggatcaacg tttttgatca tttcatgtac agtgatgaaa ttccctagag
    45241 atttactcat tttttcatcg tcttcgccaa tcgttacata gccattgtgc atccagtaat
    45301 tagcgaattt tttccctgtt ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa
    45361 attctaaatc ttgcccgcca ccatggatat cgatcgtttc tcctaaatgt tttgttgcca
    45421 taacggaaca ttcaatatgc caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa
    45481 tctcgccagg ttttgcttgt ttccataaag cgaaatctaa tgggtcttct ttttgctgct
    45541 gttcttcccc tgtacgctga cttgctccga cttccagttc atcgatggat tggtcactta
    45601 atttcccata attttcaaat ttacgggtac ggtaatacac atcacctgaa gattcataag
    45661 catagccttt ttcaaccaac acttgaataa atgctaaaat atctggcata tgatccatga
    45721 ctcttggatg cagtgtagca ggttcgacat tcaacgcttt cgtatcttct tcaaatgctc
    45781 ggatgaaacg atcagcaact tctggtgctg taatacctaa ttcgtttgct gtacggatga
    45841 ttttatcgtc tacatccgtg aaattcgata cgaaatcgac tttatagcct cgatattcaa
    45901 aatagcgtcg aattgtatcg aaagcaatgg tgctacgagc atttccgata tggatataat
    45961 tatacacagt cggtccgcag acatacatac cgacatgtcc cttttgtata gggatgaatt
    46021 cttctttttt tctggtcaat gtattgtaga tttttatcat tctgatctcc tcactttttc
    46081 tcctttgatt cgaacgactt ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc
    46141 tttcaacaca acggcacctg caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa
    46201 tatctgcgca ttcgcagaga gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt
    46261 ttcttttcct gtgccgccta atgttacgcc atggaataaa atcacatcgt cctcgatttc
    46321 tgcggtttcg ccaatgacga ttcccattcc atggtcgatg aacacgccag ttccaatcgt
    46381 tgctcctgga tggatctcaa caccagttag gaaacgccag aattgagcat tgatcttcgc
    46441 caataaaaag agccggtgct gatataaaaa atgagagaaa cgatgccaaa acaaggcatg
    46501 aaaaccaggg tatgtcaaaa cgatctcagc tgtcgaacgt gcagccggat cgtttctctt
    46561 tgctgcatca atcgctcggg taaaccaact catgactttc cactcctttc gtaagaattt
    46621 tatgtaggaa atcatcttct tataaagaaa agaagcgcct tttaaataag tttccttatc
    46681 aaaagacgct ttccgcgtgg ttccactttt cttcataatc aatgattatc tctgacagct
    46741 aacgcctgtt atacgttctt ggctactttg tttcctataa aacagttttt cctgttttct
    46801 gtgtttcgcc aagaccactc ccagaggcat ttcggcaacc attcattgat accttgcacc
    46861 acccggtatc tctcttgtaa tgatcagctg cttacttttt tctgttcatc gtgtttagtt
    46921 cagtacttga tttaaatgtg ctaatgattt ttcacgacca agcaattcga tcgtttcagg
    46981 caattctgga ccatgcattt gtcctgatac agctacacgg attggcatga aaagattttt
    47041 tccttttacg ccagtttctt tttgaacagc tttgatggca gctttgacag ttggagcatc
    47101 tactacttcc atttgttcta gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc
    47161 accagcaagt acttcttttg ctgcttcatt caatacagga tgttcattga aaaatagcgt
    47221 agacagatca acgatttctg cagcatagct catttgaggt tgatacaggc taacgatctt
    47281 cttcacccat tcgattgtct tttcatctgg gttttctgct acacgtccat cagcgattag
    47341 ataaggcaga cacatgtcag taaatgcatc tagatccatt gctttgatgt aatggttgtt
    47401 tacccattgc agttttttcg catcgaaagc tgcgggtgat ttacttaggc gttttggatc
    47461 gaacattttg atcaattctt ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc
    47521 caataatgca ataaagttga acattgcatc tggtagatag cctaattcgc gatattgttc
    47581 gataaattgc aagattgatt cgtcacgttt gcttagtttt ttacctgttt cactgttgat
    47641 gatcaatgtc atatgtccga aggttggtgg tgtccagcca aatgcttcat agatcatcag
    47701 ttgttttggt gtattggcaa tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa
    47761 atgatcatcg accgcaacag caaaattata agttggcata ccatcgcgtt tttggatgac
    47821 aaagtcgcca ccgatattat cggattcgaa cgaaatagcg cctttcacca tatctgtaaa
    47881 tgcgtattct gtattgcgag gcacacggaa acgtacaaca gattctagac cttgtgcttc
    47941 tttttctgct tgttcttcag gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc
    48001 tcctcttgcg cgttgtgctt cccgttctgc ttctaattct tcttctgtgc aatagcattt
    48061 gtatgctcga ttgctagcaa gcagctgatc aatcagcggt tgatagattt ctttacgttc
    48121 agattgacgg taagggccgt attctccagg attttcagga gattcatccc aattcattcc
    48181 caaccaagca aggttttcca actggctttt ttcgccatct tcgatattac gtttttggtc
    48241 agtatcctca atacgaataa taaagtctcc atcattgtgg cgggcaaaca aataattgaa
    48301 taatgcagtt cttgcatttc caatatgcag gtgcccagtt ggacttggtg catagcgtac
    48361 gcgtactttc gtcattttct tcctcttctt tcaaactaga ctttgactaa cagcaaaaat
    48421 tccgtcagtt acggcttctc aaatatcatt tatttaaagc cactcagcac aaattttacc
    48481 ttttctaagg taaatagtaa agccaactgg ttgaatttcc ttaccagttg gctgaatttc
    48541 ttttttattt tgattgttta gtctttttct ctgtctcttt tttattgtcg attcctcgtc
    48601 ctgcatgtgc aggtttagca aagatcatgc gtccagcagc tgtttgaagg gcacttgtta
    48661 caacgacttc gatacgttca ttcatgtaat gctgtccatc ttctacgaca accatcgtgc
    48721 cgtcatcaag gtaggctact ccttgttggc gttctgtccc ggcttttact actagaacat
    48781 tcatcgtttc accagggatg acgactggtt ttacggcatt tgccaacgca ttgatattaa
    48841 gaacaggtac attttggaat tcagagactt tattcaagtt atagtcattg gttacaatga
    48901 caccatctaa aagcttagcc aatttgatca atttgctgtc tacttcactg atatcttcaa
    48961 agtctccatc gtacatttcg acagatatac cctcttcttt ttgcaaggca tttaggatat
    49021 ctaatccgcg acgaccacgc acacgtttca aactatcccc tgagtccgcg atgtattgta
    49081 gttcgtaaag aacaaaatta gggatcatca aggttccttc taagaatcca gtctttgtaa
    49141 tatcataaat ccgaccgtca atgatcacgc ttgtatccaa gattttatat ttatggaaat
    49201 tttctcctga ttttcgttct aacagttgac tctcaacttg actagctgtt tcttgctcga
    49261 tttttttgct tcgtgaacca aaaactttct tccattcttc gattcgtgtc gttcccattc
    49321 tcaatcccag atagcctaag atgatcatca gtaaaatcgg caatacacta ttgacgaatg
    49381 gaatttccat attgtaaaga ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg
    49441 caccgatcgc accgaagaga agataagtca agctgacctc acttagtttt tgttctactt
    49501 gtttgataat agctgagata tgtttggcca acagcaaaga taataaaaag aaaataagtg
    49561 cgcctaacag gctgttggta aatgtattat tcagccatcc gtttgactgt tgaccgattg
    49621 cctgccaagc tgagggtaaa aatgaaatac ccaagcttgc gccgacaatg atcatcagca
    49681 aagtaatgac acgtttttgc atcgtttttc ctccgtttcg acaatcattt attagttagc
    49741 ggaacacacg cttcaacgtt tcagccaaag ttgctactcc aactatctcg attccttttg
    49801 gcgcttccca gttacctaag ttgtttttag gtaggtatac ttttgtgaag ccgagttttt
    49861 ggacttcttt gactcgttgt tcaatatgac tgacacgtct gatttctcca gtcagtccaa
    49921 tttcaccgat gaaacattcc gttggctgtg ttcctttttc tttatagctt gaagcaatac
    49981 tgacagcgat agctaaatca atcgctggtt catttaattt taccccacct gcagctttta
    50041 agtaagcatc ttgattctga agaagcaacc cagctctttt ttccagcact gccatgatca
    50101 gagatacccg attgaaatca agacctgttg ttgttcgttt ggcatttccg aacatagtag
    50161 gtgtgactaa tgcttggatc tctaccaaaa ttggacgtgt cccttccatt gccacaacga
    50221 tagcagagcc agttgcaccg tctaggcgtt cttctaagaa aatctgtgaa ggattagcga
    50281 cttcttccaa tccatgctca tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc
    50341 ggttttttac tgctcgcaaa atccgaaaac tatgatgttg ttcaccttca aaatataaca
    50401 ccgtatccac catatgttct aacattcttg ggccggcgat cgagccttct ttggtaacat
    50461 gaccgacgat aaaaatggct atcccatttg ttttagcggt tttgagtagt tctgcagtgg
    50521 tttctcttac ttggctgaca cttcctgcca cacttgtgat gtctggctgc gtcatcgttt
    50581 ggatggaatc aatgataaca taatcagggg agatatgttc gatggcacga ctgatttcgt
    50641 tcatatctgt ttcagcgtat agataaaatt ctgtgttgat acttccgagg cgttccgcgc
    50701 gtaattttat ctgctctgcg ctttcttcgc cggatacata taagactttt ccgcctatgg
    50761 ctgctaattg ctgagaaact tgtaacagca aagtggattt cccgatacct ggatcgccac
    50821 caatcaagac catagaccct ggcaccacac ctccgcctaa cacacggttc agttcttcta
    50881 attttgtttt gatccgaggt tcttttttag ggacgacgtc tgcgattttt gttggttttg
    50941 ctttttctcc tgtcaaactt gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt
    51001 cgaccatcgt attccatttt ccgcaatttg ggcagcgtcc caagaactta ggcgacacat
    51061 acccgcaatt ttggcaaaca aattgtactt ttgatttttt tgccattgta ccgcctcctt
    51121 ttatcctaat catgagacat taaatcaagc ttaagtatct ttaacgtttt atttactgga
    51181 agaaccaaat ccgccttgac gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg
    51241 caaaaagatc ccttggccga tacgctcgcc ttttttgatc actacatcct tgaaaccaaa
    51301 gttggtgaat tggaacatga tatggccttc atttgcctca ttattgtaat aatcactgtc
    51361 aatcacacct actccgtttg tcaacactaa aaaccgcttc aacggattgc ttgaacggtt
    51421 agcaagctgc aaaaattcat cttctcccat gtaagctttg attccagtag gcacgagggt
    51481 tggtttcaat tgtttttcat tttctgatac atcttttttc aatccatctg cgactgtttc
    51541 caaccccttt ttccagcctg ctttccagat acttggaatc acgacctctt ctgctgcttc
    51601 aaaatcatag ccagcagcat gatgtgtagc gcgctgcggt agattgatat tttgatcttt
    51661 atatttagaa ataacttcaa atcctctttt tctcaccggt tttcctccct aaataaacta
    51721 ctcctattct aacatatccg aggctgtaaa agactttctt tacaaattct tcaagaaatt
    51781 ctgctatctt aaagtaaacc attaattcgt tctttttcca aacgactgca agttgtacaa
    51841 aaaaactatt ttttacatga ctttactcgt caaaacaagt agaatagaac gaaattaatg
    51901 agtggatatt tgattgaggt gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat
    51961 tgtcaaagta ggaacaagta cattgattta tcccaatgga aatatcaatc ttgcggcaat
    52021 cgatcagtta gcttttgtcc tttctgatct tagaaatcgg ggaaaggaag tgatcttagt
    52081 ctcatcagga gctgtaggcg ttggtatgca taaattacat ttgtcagaac gtccgacttt
    52141 gattcctgaa cagcaagctg cggcggctgt tggtcaagcc gaactgatga atatctataa
    52201 tcaaagattc agtgtatata atcaacagac ggcacagatc ctcgtgacaa gagacgtggt
    52261 agattatcct gaaagtcgta aaaatgtgag gaatacattg gaacagcttt tgattatgga
    52321 cacgatccct atcgtgaacg aaaacgatac tgtagcagtg gatgaattag atcatctgac
    52381 aaaatttggc gataatgatc agctgtcagc catcatggca aaacttgtcg cggcagacct
    52441 tttgatcgtc ctttctgata tcgacgggtt ttattcagat aatccgacta caaatcccga
    52501 ggctcagatg tatcgctata tcgataaaat cgatgaaaag attctggcac aagcaggtgg
    52561 tgctggtagc acatttggaa caggcggtat gcagagtaaa ttaaaagccg cttctaggat
    52621 atttgaagca aacagcgcaa tgattttagc aaatggaaaa aatccggcaa ttatttttga
    52681 tattcttgca ggaaaagaag taggtacttt atttaaggag gacttgaaat gactgacttg
    52741 atgcaattag gacaacaggc aaaagatagc gcgcaacaac tagcacttat gcctacaaac
    52801 cgcaaaaatg agctacttgt tcaaatggca caaaccatca aaaagaatca acaagcgatc
    52861 attgaagcaa atcataaaga cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg
    52921 caagaccgct tgcagctgac acctgagcga atcaatgcca tggctacaga gatcgaaaaa
    52981 attgcttcgt tagacgatcc tattggaaaa gtagatgaga tgtggacgaa caccgacgga
    53041 ttgagaatcg gcaaaaagcg cgtgccacta ggcgtgatcg gcatcattta cgaatctcgt
    53101 ccaaatgtca cgacagatgc agctagtcta gcattcaaat cagggaatgc cgttatcctt
    53161 cgcggtggca aagaagcatt cttctcgaat caattattag tccaattatt gcagcaagta
    53221 ctggtcagtg ctggcgaatc tccttatgcc atccagtttg tcgatgatac ttctcatgaa
    53281 actgcaggac agttgatgcg attgacagaa tacttagatg tgctgattcc tcgaggcgga
    53341 gcaaaattaa tccaacgagt caaagaacag gcaacggtac ctatcattga aacaggtaca
    53401 ggaaataatc acgtatatat cgataaagaa gcacagttat ctatggcagt gaacatcatc
    53461 gtcaatgcaa aagcttcccg tccatctgtc tgtaacgcgg cagaaacatt gctgatccat
    53521 tctgagatcg cacctttttt cttgccagct attgaaaaag aactggtaga acacggggtc
    53581 tctttacgtg cggatgctcg tgcgttagaa tatctagaga cggctgcttt agcagaagac
    53641 acagattggg atactgaata tctagattat atccttgctg tcaaagtggt cgattctttg
    53701 gatgaagcaa ttgcacatat caatcggcat aatactaaac attcagaaac gatcgtgaca
    53761 gatagttatg cggctagtca acgtttctta aatgaagtgg acgcggcggc tgtctatgtc
    53821 aatgcctcca ctcgttttac agacggatcg gtatttggtt ttggtgcaga gattggtatc
    53881 tctactcaaa agttgcatgc ccgtggtcca atggggttaa atgaattaac ttccacaaaa
    53941 tatatcattt atggcgatgg acaaatccgc gaataatgtt tcgtgtgaaa cattattccc
    54001 gccttttgcc ttttttctgt aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa
    54061 attttaatga ttttttttat ttaccttatt tctctgaata tcgctgcttt tctgggagtc
    54121 ggtattttat cccttttctt tcaattcaaa aaacgaagca tagggagtca acgggaaaaa
    54181 tggtcgcagt attttgacaa aatcgggcaa aaagggctgg ttacacgctt gcacattagt
    54241 tacatggttg cattatgctt attagcgaca atcaattact attctttttt cgatcattca
    54301 atcgcttata cgattacgct actgattgct gggatttttc acttatctta taaatatcag
    54361 ctgaataaaa accacttgaa tcggactttc cgctaaaaaa tatccgtacg ataagtagag
    54421 tgccttgaag atcggcaaaa aactgccgga ctttttagag gctgtgacaa agattttgtc
    54481 acagcctctt tttccatata aacggcgttc gcctaccagc tgcctggtaa atctgtttcc
    54541 tcttcgttaa gaagcgaaaa tccgtcaaat gtttcgccat cataataacg aagcagctca
    54601 tccatggaca agttcatcgt tttttctttt tcttcttccg ctgattcagc cagtagctgt
    54661 tcccgataca cctgatacat accagctatt ttttgctgca tcgcttttct agtttcacct
    54721 tccgcattga ttattggcaa ttctttgata tagccacgat aaatcaaccg atcatctgaa
    54781 aattcaatgg gtaattcaac gagtgtcaac ccgacttcct gaaaatattc ttccagcatc
    54841 tattatcccc ccattctttt tcttaaatga attttagctt acctactgaa aattttccag
    54901 tttcattatt ttgagaaaaa aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc
    54961 gaataaatgg tgttcttctg ccaactcctc aatcataagt cacaatattc aacaaacatg
    55021 agaaactgtt ttttgaaatt gttccttgtg attcagagtt aaacgacagt ctcacaacct
    55081 cttggtaaac ggcgttcaat cagctgaccc tcggtattag ctcaaaaaac ggtaaaatat
    55141 gagaagctat ttttccgttt tgtttctcta atacttagga taaaacgctg attttacaac
    55201 ctctgttaaa cggtgttcaa tcagctgtcc ttcggtatta gctcaaaaaa cggcaaaata
    55261 tgaggaacta tttttccgtt tgtttctcta atactcagga caaaacgctg attttacaac
    55321 ctcctgataa acggtgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
    55381 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
    55441 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
    55501 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
    55561 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
    55621 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
    55681 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
    55741 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
    55801 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
    55861 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
    55921 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
    55981 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
    56041 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
    56101 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
    56161 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
    56221 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
    56281 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
    56341 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gtttttttca
    56401 aaccgatccg actagataag aaacctgtaa atagcatcac cacaccagtg actaacatgt
    56461 aactagtaaa taacagggtc gtttcagctg gcgtagcgtt taactgtgca gcaatcgttt
    56521 ttaagattgg gtctactaga cccactccca taaaagcgat catacaggta gctgctacaa
    56581 ctagcgctga acttttcccc tttgtttctt catttgtttg ttcttgtata tctatttgat
    56641 tctccaatat ttccatccct ttctctctat tagcatcata catgatttat tttacatgtc
    56701 aatatgcaca tgtaaaacgt ggaaacttta ctgtgatata ataaagaaaa taagacgaga
    56761 ggtaggattt ttatgaatac tttagcctat atattattat gcgtgctttt atccaaacca
    56821 cgttctgggt atgaattgaa gcagctgatc acaatttttt gggaagccca tcacagtcag
    56881 atctatacaa cgcttgccaa gttagaccag cagggttatg tgaccgtttt ggacaaagac
    56941 gagcactctc aaaaaaagat ctaccacttg actgatgaag gacgtgctct tgtagaggaa
    57001 tggatcaaag aggaaactcc cgctcctacg caaaaagatg aatttctagc aaaaatctat
    57061 gctttttcga cgctcgataa aacaacagct actggactgc tattcactag agaacaacag
    57121 ctgaacaaaa tcatgcaaaa aaatcaaaaa aaattgaatg acctctcgct agacaagaaa
    57181 caagaatttg gacgttatgt tgtgatccag cgcagaatta tgctttgtca gcaggaactc
    57241 caatggtgtc agcaagtaag agaacaaatg cagcagtttt ttgagtaagg gataaacaga
    57301 acctttctag aaatatctat tgaagagcaa attatcttca ataaaagagc aatacagttg
    57361 gaaaagaaaa agcgatccga acgacaggcc aaaacctatc gttcggatca ctatttttct
    57421 agaactgcat tcaagagact atgacaaaaa gcatgccaag gccttttttc aaataacagt
    57481 gttccatcat tcattacttt gaattgcttt tagcacactt cttgaaagcg ggccaacgat
    57541 gatcaactgc aacggtaaag cggcaatcac attcattttc caagcattca tataagcaga
    57601 gaataattga tcaggtattc cttgttccat gatcacaccg aataaagaca tacatgtcac
    57661 cattcctaaa accatcaagc atgaaatagt taagatcatt tggaccttgc tttctggatt
    57721 tactggtagt ttaaaggcaa tttttttcgc cagcacaccg atgatgaaga catccaaaat
    57781 gaaagccacc acgaatccag gcactagacc aatcaacaaa cttgataaat gaagatcttg
    57841 atgcagccat aaattatacg cgctcatccc caagaccatc aaaaaacaca tcattgttgt
    57901 aaaaattatt ccttcttttt tatttgtagg caatctgtac cactccttta aatttgtcac
    57961 gaaaatcatc gtagcacatt taaaaatttc tcgtaagtac tttttttatt ttctgccctt
    58021 ttatttcata aacttcatca gccgtacgct cgacaaagta agaatcatga gaagtaaaca
    58081 aaatcgttcc cggatatatt tgcattagct tctctaaagc ttcgatcgtt gccatatcaa
    58141 taaaattagt cggttcatct aacagcaaca cattacttgg cttagtaaaa agcaaagcaa
    58201 tcgtcagacg ggtcgcttct cccccactta atgtacataa cggtttcgtc aaagtttcct
    58261 gagcaaaccc taagtgattc agcaaactac ggacaagcga ttctgaaaac tctgtttgtc
    58321 tcatgacaaa acgaatgatg gattcttcag atgtcatttg ataacccttt tgctgatata
    58381 cttgaaagct tactttcgga gagagaagga ttccttctcc ttgtttgcgg atatgttcta
    58441 ataaggttgt cttacctgaa ccattttcac cgacaatcgc gattttttta cctaaagaaa
    58501 actgaaaatc accatttacc agtaatgttc tcccacttct ttccaattgg acattttgtc
    58561 ccatgatggg atagcggctg tggagttcaa gagattttgg tactggaaaa cggatttgtt
    58621 tcatctgctg cggcttggtt gtttctccta ttttttggag tcttctctct aatgttttcg
    58681 cattcttttg gatggctttt tgtacactgt ctttttgttt agaggaagac aaccgatcca
    58741 gtctgatttg ttgctttttc tttttactgg acactttttc caacttttcc gcttgttttt
    58801 tcttttcttg gatcgatttt tccaatcgtt ttttctcttt catgatcgac tctgcttctc
    58861 gtagctgagt ctttttctcc aattcctttt gagtgagata ggcactataa ttccccgaaa
    58921 actctcggat ttctccgtct tttacttccc agattttcga tgctagctca tctagaaaat
    58981 agcgatcatg actaacaaaa accagcgtac cataataata acgaagctct tcaatcagtc
    59041 gatcaatact ttttctatct agatgggttg ttggctcgtc tagaagcatc ccttgttcat
    59101 aagaagaaag aagacgtatc aactcgatct ttcgttcttc ccctccgctc catttccgct
    59161 ctgaagtttc ttccagtctt aactggaaat gactggctaa ttccaaattt aggtcctttg
    59221 cttcagcgag ataggttaat tgaggcaggt agttgaaggt gatctctgtt tggattttcc
    59281 catgatcggg aaaaagttca ccggctatta gtttgagcaa agtcgattta ccgacaccat
    59341 tttctcctat gatccctata cgatcttgct gatagacacg aagagaatca atagatagtt
    59401 ctagctggtt gccaaagctg acttgtagat ttgttatatt tactgctaaa ttttccatat
    59461 gttttacact cctatttcat gtaaaacaac aattatcgat actaaaaaag agcagaaaaa
    59521 tctgtttgca aaaaaactgc atcacatctt ttcctactct gaacgaaata actatgacta
    59581 aatcaccaaa tctgtcatag ctttcaccta ttctactgtc gttcgaaaaa atggacatac
    59641 tccatccatg aaattgttgt ttatacatgt cttttgaatt tatttagttg ttattcaaaa
    59701 gttcgaaacg taatttcatc gatgcagtca tttttccgac ctcctttttt attaggttac
    59761 cacacacgat tcaaaaaaag caatagctac tgaatagtaa ctgaaaacct tttattcgaa
    59821 catagtgatc ttttctggtg ctaaacgaac agactcgtaa ccttcttcta ccgcttttcc
    59881 tattgataaa ataataactg gaacataacg gtctttatcc aaaccaaatg cctctgccaa
    59941 ctgatctgct tcgaatccac caattggatt tgtttcatag ccatgatcac gcgcaactaa
    60001 catgaattgc atagccgcta agctagaatc gactttgact acatcgttca tttcttctct
    60061 tgaaaaactc ttgtaataag gaatgatcgc agctaattgt tgatctctga cttcttttgg
    60121 catcttccct gattcgtaag cttggttgta gatttcttcg ccatattcgt aacattgcat
    60181 atcgccaaaa atcatgacca ttgcagaaga agtatcattt tgtctcgtat tgaaacggat
    60241 caaaggacgc aagatttctt ttgcttcctc gctttcagcg accacaaagc gccatggttt
    60301 catattgaca gaagaaggtg cagtcgtcgc ttctttgatc atttccagca tttcttcgtg
    60361 tgggattttg tatgtttcat catatacgcg aacggatctt ctaccagtca taattgttga
    60421 aaaatcattt ttttgttcca ttattattaa cctctttcgt ttctgttgtt atattgcaaa
    60481 gttgggtatt caatttatct aaaagtgtta gaaattgttt catttctaca tcgctgaaag
    60541 ccggggatag cacggaacct gtatctttat ggtccatttc acaatgcttg attttcttct
    60601 gggctttttc tgttagcgct acatacactt cccggttgtt ctgaggattt ctctgacgcg
    60661 aaacatagcc atttgcttcc aataccttca gatgacgagt aactgctcct tgatcaatct
    60721 gcaacttttc ttgcaaactg ctttgtagca atggattttc ttctttcagt ctcatcatga
    60781 tctcgtatct agtaagactt ataccgatat tttcttcaaa gatctggacg attttttgat
    60841 cggctaattt gatttgatat aaaaggtgac taatctcttc taaagacata atttcctcct
    60901 ttctaataat tgactcgtca acgattgact tgtcaataat agaataaaaa aagaaaaaag
    60961 tcaaagaatc agctgtgtaa taaaaaaaac gctctcaata gaaaagtata gagaaaagaa
    61021 attttttgta gaatgaacgg aaaaaatgat aaagaaagca caacgctaaa accaagatta
    61081 ttgattctta tcaaactata gaagatagct cttctgcagt agataatttt acaccgccaa
    61141 aaatttccga aatcgccttt tgcaagaatc ccgtcgaata ttagaaattg gatcagtatg
    61201 cttaaacagt aataaaaaag ttatgacaag atgttgtcac aacctttttt gagtaaacgg
    61261 tgttcaaaaa cttgcatctg cggtaataag ttcaaccaaa caaaaaacat gaaaatctgt
    61321 ttttgtttgg ctgttcttac tacttgatgc agggcagctt tttcactacc tctttttaat
    61381 aaccataaaa tcctattcct tcatatctcc agcccatcgg tgttagccaa tctctttcct
    61441 tagcactcaa ggtatagtga tgggtaccag ttttttcatt tggattaaat agtcgataaa
    61501 ttggcttgga accaccagaa taaaaactta gaccttcata acgccaaccg atttttgtta
    61561 aagagttgat ctcagattgt gccattgtgt aatgatggtc tccactgtaa ggattgtata
    61621 aacggtagac aggttgtccg ctgtttggtg cttgccaagc aatcccttca tattcccagc
    61681 ctacagttgg cagataattt ttttctccgg cattcaatgt atataaatgt tctcctgtat
    61741 gacggttata cagacgatac atcggagaag ttgaagttgg tgctgtatca tactgtgtca
    61801 gattataggt ttcgattaca ttgttcaatg tagaaccata attaggcgca gtggcataac
    61861 gacctgttag ccaagccgta gcatctttgt aagagttcgt attgcttttc catgcaccgg
    61921 aatagtagta aacgcctggc tgaaaagaag tcgtcttcaa taccgttacg ttatcttgca
    61981 atgattcttt gtacgaagga tattttctaa aagcggcatt caccgtaatc cattgcccat
    62041 tcacatattc cgaagtaggc atcgtaacag attggccatt atagcttcct ttaatcccaa
    62101 atagattgta attaggggca gaagccaaag tactgttccc ccagccactt tcaacgatgg
    62161 cttgagcaat catgactgat gcatataagt cattggcact tgccaaactt tgtgcagttg
    62221 aggcaatact atcaatgaac gcttgttgag aattagcaga tgagcgagca taaaaagcat
    62281 agccagtata gttttcgaat tcagacaaat ctgcttgatg ggtatgtgtc aatgtttgcc
    62341 catctgtcaa ttggttcgtg atttcttctg cattctctgg attgttctga gcttctgctg
    62401 atgtactgtc ttcggtttgt gtttgttctg ttgtttcagt tgttggttct tcggttgaaa
    62461 ttgtattttc tgagacagta gctgactgca aagtactttc tgtatcgttt tcaattcctg
    62521 tttctgaaga actcgatgtc tgagttgcga cagtttctgt cgattcttca gctagcactg
    62581 ttgtgttggc aactgctaga tttcctaaaa gaataacgct aaataataaa ttctttgctt
    62641 tcatcttttt tctcctttga attatgaaac ttttcctaaa taacagtgta actaaaaaag
    62701 taaaagcaag tcaatgtcca tatttaaata acatttgttt ctttttaata aatttgcttt
    62761 ttctgcataa aaagacgtaa atgaagaata ttcttctcca ttcacgcctt aaattgatgg
    62821 tcgtttgaat aactatttaa caagtaattc tctctggata ttatccgtta tggattatac
    62881 ttcctgtgtc gtcggacgga taatcatgtt gctgacagac atccgttctg gcgtttctat
    62941 cgcaaatgct acagcagaag ctatatcttc agatgttaat ccccattctt tttgcgcttc
    63001 atgaagttca agagcagcct ttttgtcgct gatggtttga tataattcag tttgaacagc
    63061 tcctggagag atgatcgtag atttgatgtt attttgccgt tgctcttgac gtaatccttc
    63121 catgattgca cggacagcaa attttgttcc gcaataaacg gcggaatcag gataaacaac
    63181 atgtcccgcc acagaatcag ttgaaatgat atgcccagat ttttgttttt ccatgattgg
    63241 aagtacggcg gaaattccat ttaacacacc catgatgttg atatcaagca tattttgcca
    63301 ttcatcacgg tgaccttcaa ctaatggagc ggttggcatg attcctgcat tattgtacaa
    63361 tacatcgatt cgaccgaact tttccatcgc taaatcgata actgcttgga cttgtgcaaa
    63421 atctcttaca tctgctgttt gaatatatag ctcagcttct ggtaaagatt cttttattgc
    63481 ttttaggcga tcttctcggc gtgcagctat aactagttta gccccttttt ctgctaatag
    63541 cttcgttgtt gcttcaccga ttccgcttga agcgcccatg atgacaatca ctttttctga
    63601 taaatcattc attctattct cctcctgttt tgaaaaatct cttgcttata ctcactatct
    63661 cttatttcat cttactgttc aaataatccg ttcgacaggt ttgacgattt tggcagggat
    63721 tcctgcaacg atggtattgt ctgggacatc tttcgtaaca gttgcattcg ctgcaacaat
    63781 ggcattctcg ccaatcgtta cgcctggcag aatagttgct cctgctccga tccaagcatt
    63841 tttcttgatg tggattggct ttactgacaa gcctcttcgt tcttttggat cggttagatg
    63901 gttcaccgtg atgaggttga cacgaggacc aatcaatact gaatcttcta aacagatgcc
    63961 acctaagtca acgaacatca cttgacgatt gataaagaca tttttcccta tactaatatg
    64021 tttgccgaaa tctgtataaa aaggcagcag aacagtcacc gtatcatcta ctttgtcact
    64081 aatgatattt tctagccgtt tgcggatatc tgctggttca tggtacccag tattcagcgc
    64141 tgctaaaggc tcttcgtttt ccgcttggat ctgatggatc acttcaaaga gcggatccgt
    64201 tactcgaatc tctttattga cgatttgttt ttcgatgagg cttgtcattt tgctccctcc
    64261 ttttatcctt actttataac ggacggctta tgatgtctaa tacttataaa acatacttaa
    64321 ctattccttt taactatact tagtggtatg cttttttaaa aacaggaggg aaaaaatgga
    64381 tattcgtata ctgacgtatt ttttagcagt agctagagaa aaaaatatca gccgagcagc
    64441 aaaatctctg catatcacgc aacctactct ctcaaaacaa ctgaaagatt tagaagaaga
    64501 acttggtgtg acacttttca ctcgaggctc ccgagagata cagttgacgg attctggtcg
    64561 atatctttat tcaaaaggaa aagaaatctt gtcgttggta gaaaaaacgg cgaacaattt
    64621 aacaaatgac gcaatcatct ctggagaaat ctacattggc ggaggcgaaa ctaaagcaat
    64681 ctctttcatt tctgatagcc ttcattcgtt gatcaaggcg catcctgata tccagtttca
    64741 tttatatagc gggaatgcag atgatatcat agacaaactg gatagcggat tacttgattt
    64801 tggactcgta atcgaaccga caaacaaaca aaaataccag tcatttcgac tgccagccca
    64861 agatcgttgg ggacttttga tacatcggaa tcatccttta gcaaagcaaa cagtcatcca
    64921 tccgcaggaa ctgaaaactg ttcctttgat catttcccgg caaacgtcag tggacagtca
    64981 actagctgaa tggctaggtt tcagcataga tcagttatcg atcgtcggaa catataactt
    65041 attgtacaat gcttctttga tggtcgataa cgggactttt ggtgcattag ctctagatgg
    65101 gatcatcaat acttatggga cggaaaaagt attcatccca ctcgatccgc cattacaagc
    65161 ttcactgaat ctcatttgga aaaaagacca gcctctttct cctgctgctc gtaaattttt
    65221 agatgtccta acggagaaat acagttaagt agagtttttt tccttcgctt tcagcttttt
    65281 aacaacgaat aagttaagca aaagatccaa tgattaaata acataagttt cttaatttgt
    65341 tccttccttt tatttctgat aattccagta aattactata attataaaaa aagaattgac
    65401 aaaacataac tgtctatata aaattgatta cgttaacaat aattcgtttc atgtgactag
    65461 atcatactag gcatgaaaaa tctctgagga acatggtttc cctaggaggt ttttcgtgtc
    65521 tatttttgtt tggccgcaat caactggtct agcaaacgtg aaaaccaaat cttcaaaaag
    65581 ggtgcgcgca tgaaaatcaa aaagatcttt aatcaaaatg cagtattggt agatgacggt
    65641 ggacaggaaa aagtagctat tggaaaaggg attggatttg acagaaaaag aaatgatttg
    65701 atttttgatc gtgatgtcga acggatattt gtcatggaac cagaaggtca aatcaagttg
    65761 caaaatttac ttaatcagat tgatgaacgc tttttatttg cagcagaaac gatcattgat
    65821 catgcggaaa ctgttttgat ggaaaagttg aatgaacatg tattgatcgc tttgaccgac
    65881 cacattgctt tttctgcgga gaacattaat aacggtatcg tcatccgcaa caaactttta
    65941 agagaaatcg aagtgcttta cagtgatgaa ttcagtattg ctcaatgggc agtagagtat
    66001 ctgacaaaag agcttgggat tccatatact tatgacgaag caggatatat cgcaatccat
    66061 attcacagcg gacgaagcgg tcggaacaat aaccatcgca gtatccgtga agtgacgatt
    66121 gtatctgaca tcatccattt agtggaatca gaattggaaa cagatatcca ttcagaacca
    66181 ttttcactga attattcaag actggtgaat catttacgac tgcttctaca acgaacccat
    66241 gcgcaacaat atgctgtttt agacacggaa atcgtagaaa tggtcaaacg aaaataccca
    66301 gaaagctata agatttctaa aaaaatccgt gtgttgctga ccaaagaata ccagctcgcg
    66361 atcacgaaag aagaactggg ttacttggcc atccatatcg agcgtttaag aagcgcaatc
    66421 gtacaaacaa atgttaacaa tcatgaggag gaaaaaaatt gaaagcatat ctacaacgaa
    66481 tgggacgttc ccttcaactt ccagttgcgg tcttacctgc tgctgcttta ctagtaggga
    66541 tcggaaactg gtgggcatct tatagtaacg acattgtcgc tcatttttta caagctggcg
    66601 gtaacgcagt tttaggtcaa ttacctttgc tgtttgccgt tgggttagca ctagggatgt
    66661 cagtggacaa atcaggtgct gctgccttgg caggtttggt cgcatttgaa gttccagtaa
    66721 atgttttaaa aactgattca gtagctactt tattgaatat caaaccagaa tcagttgatc
    66781 catcttttgc tcagatcagt aacgtattta ttggtattat tgccggattg attgcggcag
    66841 ctctttataa tcgtttccac gaagcaaaat taccgatggc tttatctttc tttagcggca
    66901 agcgtctagt gccaatcttg gctgcctttg tcatgttgct gatcagtgca gtgctactgc
    66961 tcgtatggcc tccggtttac tctgctttag tttcttttgc taaattcatt ttgagtttag
    67021 gctggggtgg tgcaggtctt tatggcttct tcaaccgttt actgatccct acaggattgc
    67081 atcaagcaat gaactccgtg ttctggtttg acgtagcagg aatcaatgat attggtaaat
    67141 ttttggcttc tgaaggaaca aaaggcgtaa caggaatgta tcaagctgga tttttcccaa
    67201 tcatgatgtt cgggcttcca gcaggagcat atgctattta cagaaatgca cgtccagaaa
    67261 acaaagcaaa aacagcttcc ttgatgttag cagcagcttt tgcttcattc tttactggtg
    67321 taacagaacc acttgaattc tcatttatgt tcgttgcttg gccattatat gtcttgcatg
    67381 caattttcac tgggatttct ttagcagtga gtgcattctt ccattggaca gcaggatttg
    67441 cctttagtgc cggatttgtc gattacttcc tatctttgaa aaatccgatt gcgaatcagc
    67501 cattgatgtt gatcgtccaa ggattagtaa cagcagtgat ttactacttt ggctttaact
    67561 ttgctatcaa aaaattccat ctgatgactc ctggccgcga ggaagcagat ctttcagatg
    67621 acgatactgc aacgactaat tcagacaata aatatgcggc acaagcaagc aaaatctacg
    67681 ctgcattagg cggagccgac aatgtaactt cgatcgataa ctgtactaca cgccttcgtt
    67741 tgcaagtcaa agatacaggc ctgatcgatc aaaataaaat caaagccact ggcgtacctg
    67801 gtatgaagat catcgacgga aaaaatgcgc aagtcatcgt aggtacagaa gtacaattcg
    67861 ttgcagatga gatggcaaaa ctacatggtg gtgctgcaac acgacctgca caaacaaaca
    67921 ccgtggttaa aacagaaaca tttgccggtg gaacgatcga acaagatatt tacgctatcg
    67981 caaatgggaa actgattcca atcactgaag tttcagatga tgtattcgcg gaaaaaatga
    68041 tgggtgacgg ctatgctgta ttaccagaaa atggagaaat cttctctcca atcgctggta
    68101 cgatcacaaa cattttccca acgaaacatg ctgtcggaat ccaaacagat gctggtatcg
    68161 aagtcttgct tcatatgggc atcaacactg ttgacttaaa aggcgaacca ttcactttat
    68221 atgtaaaaga aggtcaaaaa gtcgcaagag gtcaattgat tgctttagtt gatcttgccg
    68281 ctattcaatc tgcaggcaaa aacacggata tggtcgttgt attcacaaat ggcgacaaag
    68341 tccaaagctt agaaatcgaa cccgcacgtg atgtaaaagc aaatgataaa atcggctctg
    68401 tttccaacaa agcctgagcc atcgtcttcc ccagactttt ttatcgataa acaaacagga
    68461 gctgagacaa aaatcttgtc tcagctcctt tttccgaata aaaacggcgt tctcctgcca
    68521 actccgcggt aataagtcca gccaaacgta acatatgaag agctattttt tatttgactc
    68581 ttcttattac ttgatgcaga acagcttttt tacaacctct gataaacggt gttcaaaaac
    68641 cagtttatcg gtatgaagtc aaatttatgc aaaaatgtgg aaagccattt tcacaaaatt
    68701 tttcttcata ctcaaaacta atcggctttt tcacaacctc tttatacatt attcctgatc
    68761 caatatttct tttaatgctg cttcaagaga agttaatggt tttcccaata ctttttctaa
    68821 atcatcagag gaaatagcta actgatcctt tttaatatca tgttggatcg ctaaaaacat
    68881 gtcagctgct tcttgaggga atccggcttc ttttagattt tcgacaaacg ctgcatcact
    68941 tgcttcaata atttctaact ttttacctgt cgcacgctct aatgccttgg ctaattcttc
    69001 atagtcagtt agattgccgg ataattctaa gatttctgga gagtcacttt ctactagtac
    69061 ttttgctgca gcttctgcat attcacgttt caatgcccaa cctgtttttc catttcctgc
    69121 agcataaacg aattgaccag tttttaaagc ctgcccaatg ataggtaatt cattctctac
    69181 ataccaatta ttccgtaaaa atgtatggga aatacctgaa tccttaatca ttttttcagt
    69241 gaattggtga tctggcgcta atacactgat cgagtgatcg gcatctgcaa aacttgtata
    69301 agcgatataa gataccccag ctgcttgtgc tgccttcact acattcttat gttcggcttg
    69361 acgatttcct ggaacgccag agacaaaaag caatcgatct attcctttca acgcttcttt
    69421 cattgagtct aaatcatcat aatcaccaat acgtatatta aatccagctt ctttcagtgc
    69481 ttctcctttt tcttcactac gtactagtac atagatttca ttttgtggaa ccatcttttt
    69541 taagaattcc agcgcataac caccaaaacc tccagttgct cctgttacta aataatgcat
    69601 atttgccact ccctattctt tttatttgtt aagatttaca gctgtacatt tataaattac
    69661 acctttatca taaagagtgt ggtaagataa gtcaagcaaa aaaaatgaaa ggatgacaac
    69721 tgatgaagta ttctctgcaa tttagtgatg caatccatgt acttgcctat attgaaatgt
    69781 ttcaagatac ccgatcactt tctagtgaga tgattgccag tagtatcgaa acaaatgcag
    69841 caaatgtacg taaaatcatg agtcagctca aaaaatccgg tttgatcttt acaaaaaccg
    69901 gaaaaccgca gccttccctt gccaaaagtc cagatcagat ttctttacta gatgtctatc
    69961 gcagcatcga aggtaataca cagcttatcc aagtcgatcc aaaaacaaat ccaaattgcg
    70021 tcgttggtgc gaatatccaa gaagctttag aagaaagcta cgctcggctg cagaaaaagg
    70081 cagaagaaga aatggctaag atcacacttg atagtctgat acataaaata gccgttttgg
    70141 aaaaagaaaa aagaccagaa aacacaggcc tcgtagagag atttttataa ttctctctac
    70201 tgtgcctgca tttttctgat cggaatcgtc tacataaatt cttttattct ccgggggaca
    70261 aaagccttgt catgcccctt tttcctctct aatgctcagg tcaaagcgct gaagtcatga
    70321 cgtcttgata aacggtgttc aagtagctga ccctcggcat tagttctgaa aaatgcaaaa
    70381 tatggaaagc tatttcccat ttttcctctc taatgctcag gtcaaagcgc tactttcacg
    70441 acctctatat tatcatggtt tattgactac cggtatccag atttctccca tatatttccc
    70501 gttggtaact cccatttcga ctacagcatt tggaccccca acataggcgt aatcttctac
    70561 ttctcttaaa acatgcccaa aggccgtatt cgtcaaatca tttcttaatt cttcaggcga
    70621 ttctgctaaa gcgattacaa caagatacgt tccttttggg aatgggatga ttcgggtagt
    70681 gtttggaaca tcctgactcg tttgaacacc gacatagtac atcattttcc cgttataggg
    70741 ttcgttgact gtaaagaaat aatgattttc agcagcttgt ttcaatgcat ctattgttcc
    70801 attaccgata actttttgct caaatgcttg tttctctgca gccaaaccga caaaatccgt
    70861 attgctactc ttcaattcca caccaatacc cgtcaccgta aaatctggtt tttcttcgat
    70921 cctatacttt gccatttctc ccaccctttc tattatatta agttcatctt acataaaatt
    70981 aaagggaaat gacacgatta cgctcttttt tctagggata tttttatttt ttaactattt
    71041 ttttgctttc actgcaatcc aaatctcact gtaaacatta ctgaaatcct ctccccattt
    71101 tgtaaaagag atctcgggag cttcgactaa ctcataatct gaagaaggaa gccattctgc
    71161 atagattttc ccccaagtat cttgtaaagt ttggggaaat ggccctttat taggaaaaat
    71221 ggcccaagtg tgttcttcta tagcaagcat ttctaaatca gcgtacggat tttcttttgt
    71281 cgtagcaaag ccaatcatgt gcatcaaact gcccttttct tccaagcggg ctccatcaaa
    71341 tccataagaa acgttgatta cttgatttgg atataaatct cctaacttat gcatctcttc
    71401 cctttgagtc tccgtgatcg tctgcgctaa cttttgtatt tcttgattga ctccttcaaa
    71461 tttgacagga acttttttcg ccacacctgc cacataaaac tgttctttct tttcgatacg
    71521 aaattccatc gattttcctc ctctgacatc aataaaaaat gtcaagcgtg gaaaagattt
    71581 ttgtatgccg tatctgctga tttctgaagg cagatatcct gaccaatcac gaaaagctct
    71641 tgaaaaacct tcgactgttt catatccata tctaaacgcc gtttctgtca ctcttgatcc
    71701 atctaggaga tcgcgattag ccaaagccaa tctacggttt ttgatatagt ccattagcga
    71761 catccctgca ataaacgtaa agatgcgttt taattgatat tcagagattc ctgtattttc
    71821 ggctattatt ttataggaaa gtccttctgt caaatgttct tcgatgtaat cgatcaattg
    71881 gttcagatct ttaagcatat ttatcttcct ccttgcttgc ctttttatca tattatcaaa
    71941 gaaagtcaat tacttgatga atcaggaacc aatttagtca gatgtagaaa atgttgtatg
    72001 gatctaaaga aaattctcaa gcagcgtttc gtaacggata attggtatta tctgcctatc
    72061 tatgttttac ctttagaaaa ataaattttg gggaagacac attctaagta aacaaagcgg
    72121 taaaaatatc ttttattccc ttagttaatc cgcttactac ctactgtaca aagcaaattg
    72181 ttgttcaaac tgctgatcag aacgctgctt ggatttttct ttgcgataca tcatacctct
    72241 tttatcaatt ggaacaatta tttatttcta acaaaatcac ttcatttttg tcagataaat
    72301 aaa
//
LOCUS       DACDXE010000004        71080 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.004, whole genome shotgun sequence.
ACCESSION   DACDXE010000004 DACDXE010000000
VERSION     DACDXE010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.004"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            161..391
                     /locus_tag="IWM23_000239"
     CDS             161..391
                     /locus_tag="IWM23_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAP9882917.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            488..1789
                     /gene="murA"
                     /locus_tag="IWM23_000240"
     CDS             488..1789
                     /gene="murA"
                     /locus_tag="IWM23_000240"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9882918.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1802..1978
                     /locus_tag="IWM23_000241"
     CDS             1802..1978
                     /locus_tag="IWM23_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9882919.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2113..2409
                     /gene="yidD"
                     /locus_tag="IWM23_000242"
     CDS             2113..2409
                     /gene="yidD"
                     /locus_tag="IWM23_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAP9882920.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2593..3060
                     /locus_tag="IWM23_000243"
     CDS             2593..3060
                     /locus_tag="IWM23_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9882921.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3145..4029
                     /locus_tag="IWM23_000244"
     CDS             3145..4029
                     /locus_tag="IWM23_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9882922.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4146..4379
                     /locus_tag="IWM23_000245"
     CDS             4146..4379
                     /locus_tag="IWM23_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAP9882923.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4467..4547
                     /locus_tag="IWM23_000246"
     tRNA            4467..4547
                     /locus_tag="IWM23_000246"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4501..4503,aa:Tyr,seq:gta)
     gene            4554..4625
                     /locus_tag="IWM23_000247"
     tRNA            4554..4625
                     /locus_tag="IWM23_000247"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4586..4588,aa:Gln,seq:ttg)
     gene            5140..5847
                     /locus_tag="IWM23_000248"
     CDS             5140..5847
                     /locus_tag="IWM23_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9882924.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5907..6551)
                     /locus_tag="IWM23_000249"
     CDS             complement(5907..6551)
                     /locus_tag="IWM23_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAP9882925.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6678..6965
                     /locus_tag="IWM23_000250"
     CDS             6678..6965
                     /locus_tag="IWM23_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882926.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7073..7393
                     /locus_tag="IWM23_000251"
     CDS             7073..7393
                     /locus_tag="IWM23_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9882927.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7474..8787
                     /locus_tag="IWM23_000252"
     CDS             7474..8787
                     /locus_tag="IWM23_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9882928.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8784..9503
                     /locus_tag="IWM23_000253"
     CDS             8784..9503
                     /locus_tag="IWM23_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAP9882929.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9631..10182
                     /gene="raiA"
                     /locus_tag="IWM23_000254"
     CDS             9631..10182
                     /gene="raiA"
                     /locus_tag="IWM23_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAP9882930.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10404..12938
                     /gene="secA"
                     /locus_tag="IWM23_000255"
     CDS             10404..12938
                     /gene="secA"
                     /locus_tag="IWM23_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAP9882931.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IWM23_000256"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IWM23_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAP9882932.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IWM23_000257"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IWM23_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAP9882933.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IWM23_000258"
     CDS             14940..15833
                     /locus_tag="IWM23_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9882934.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IWM23_000259"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IWM23_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9882935.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IWM23_000260"
     CDS             17770..18627
                     /locus_tag="IWM23_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAP9882936.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IWM23_000261"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IWM23_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAP9882937.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IWM23_000262"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IWM23_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAP9882938.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IWM23_000263"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IWM23_000263"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9882939.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IWM23_000264"
     CDS             21383..22141
                     /locus_tag="IWM23_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAP9882940.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IWM23_000265"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IWM23_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAP9882941.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23069..24100
                     /locus_tag="IWM23_000266"
     CDS             23069..24100
                     /locus_tag="IWM23_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9882942.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGKLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSIFIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            24097..24864
                     /locus_tag="IWM23_000267"
     CDS             24097..24864
                     /locus_tag="IWM23_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAP9882943.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            24964..26532
                     /gene="liaX"
                     /locus_tag="IWM23_000268"
     CDS             24964..26532
                     /gene="liaX"
                     /locus_tag="IWM23_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAP9882944.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26832..27149
                     /locus_tag="IWM23_000269"
     CDS             26832..27149
                     /locus_tag="IWM23_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAP9882945.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDEW
                     SDF"
     gene            27149..27499
                     /locus_tag="IWM23_000270"
     CDS             27149..27499
                     /locus_tag="IWM23_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAP9882946.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            27666..28604
                     /locus_tag="IWM23_000271"
     CDS             27666..28604
                     /locus_tag="IWM23_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAP9882947.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            28619..29449
                     /locus_tag="IWM23_000272"
     CDS             28619..29449
                     /locus_tag="IWM23_000272"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAP9882948.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            29493..30518
                     /locus_tag="IWM23_000273"
     CDS             29493..30518
                     /locus_tag="IWM23_000273"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9882949.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            30533..31471
                     /gene="galU"
                     /locus_tag="IWM23_000274"
     CDS             30533..31471
                     /gene="galU"
                     /locus_tag="IWM23_000274"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9882950.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            31596..32036
                     /locus_tag="IWM23_000275"
     CDS             31596..32036
                     /locus_tag="IWM23_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAP9882951.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            32039..32641
                     /locus_tag="IWM23_000276"
     CDS             32039..32641
                     /locus_tag="IWM23_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9882952.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(32700..33806)
                     /locus_tag="IWM23_000277"
     CDS             complement(32700..33806)
                     /locus_tag="IWM23_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAP9882953.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            34016..35017
                     /gene="ccpA"
                     /locus_tag="IWM23_000278"
     CDS             34016..35017
                     /gene="ccpA"
                     /locus_tag="IWM23_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAP9882954.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(35354..37699)
                     /locus_tag="IWM23_000279"
     CDS             complement(35354..37699)
                     /locus_tag="IWM23_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAP9882955.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            37978..38880
                     /locus_tag="IWM23_000280"
     CDS             37978..38880
                     /locus_tag="IWM23_000280"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAP9882956.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            39093..40868
                     /locus_tag="IWM23_000281"
     CDS             39093..40868
                     /locus_tag="IWM23_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAP9882957.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(40934..42520)
                     /locus_tag="IWM23_000282"
     CDS             complement(40934..42520)
                     /locus_tag="IWM23_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAP9882958.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            42771..42995
                     /locus_tag="IWM23_000283"
     CDS             42771..42995
                     /locus_tag="IWM23_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAP9882959.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            43137..44888
                     /locus_tag="IWM23_000284"
     CDS             43137..44888
                     /locus_tag="IWM23_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9882960.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            44888..46672
                     /locus_tag="IWM23_000285"
     CDS             44888..46672
                     /locus_tag="IWM23_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9882961.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            46998..47849
                     /locus_tag="IWM23_000286"
     CDS             46998..47849
                     /locus_tag="IWM23_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9882962.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            47903..49654
                     /locus_tag="IWM23_000287"
     CDS             47903..49654
                     /locus_tag="IWM23_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9882963.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            49658..49828
                     /locus_tag="IWM23_000288"
     CDS             49658..49828
                     /locus_tag="IWM23_000288"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882964.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(49868..50770)
                     /locus_tag="IWM23_000289"
     CDS             complement(49868..50770)
                     /locus_tag="IWM23_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9882965.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            51001..52404
                     /locus_tag="IWM23_000290"
     CDS             51001..52404
                     /locus_tag="IWM23_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9882966.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            52404..53837
                     /gene="ascB"
                     /locus_tag="IWM23_000291"
     CDS             52404..53837
                     /gene="ascB"
                     /locus_tag="IWM23_000291"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9882967.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            53982..54908
                     /gene="trxB"
                     /locus_tag="IWM23_000292"
     CDS             53982..54908
                     /gene="trxB"
                     /locus_tag="IWM23_000292"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAP9882968.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            55122..56849
                     /locus_tag="IWM23_000293"
     CDS             55122..56849
                     /locus_tag="IWM23_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAP9882969.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            56921..57214
                     /locus_tag="IWM23_000294"
     CDS             56921..57214
                     /locus_tag="IWM23_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9882970.1"
                     /translation="MPDTQKSKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(57306..58166)
                     /locus_tag="IWM23_000295"
     CDS             complement(57306..58166)
                     /locus_tag="IWM23_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAP9882971.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            58258..58686
                     /locus_tag="IWM23_000296"
     CDS             58258..58686
                     /locus_tag="IWM23_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9882972.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            58848..59024
                     /locus_tag="IWM23_000297"
     CDS             58848..59024
                     /locus_tag="IWM23_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAP9882973.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            59051..59497
                     /locus_tag="IWM23_000298"
     CDS             59051..59497
                     /locus_tag="IWM23_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAP9882974.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IWM23_000299"
     CDS             complement(59651..61030)
                     /gene="brnQ"
                     /locus_tag="IWM23_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9882975.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            61736..62707
                     /locus_tag="IWM23_000300"
     CDS             61736..62707
                     /locus_tag="IWM23_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAP9882976.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            62731..64923
                     /locus_tag="IWM23_000301"
     CDS             62731..64923
                     /locus_tag="IWM23_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAP9882977.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            64940..65416
                     /gene="ybeY"
                     /locus_tag="IWM23_000302"
     CDS             64940..65416
                     /gene="ybeY"
                     /locus_tag="IWM23_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAP9882978.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            65394..65795
                     /locus_tag="IWM23_000303"
     CDS             65394..65795
                     /locus_tag="IWM23_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAP9882979.1"
                     /translation="MPMDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            65810..66709
                     /locus_tag="IWM23_000304"
     CDS             65810..66709
                     /locus_tag="IWM23_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAP9882980.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            66852..67664
                     /gene="recO"
                     /locus_tag="IWM23_000305"
     CDS             66852..67664
                     /gene="recO"
                     /locus_tag="IWM23_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAP9882981.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            68048..68965
                     /gene="glyQ"
                     /locus_tag="IWM23_000306"
     CDS             68048..68965
                     /gene="glyQ"
                     /locus_tag="IWM23_000306"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAP9882982.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            68967..71042
                     /locus_tag="IWM23_000307"
     CDS             68967..71042
                     /locus_tag="IWM23_000307"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAP9882983.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        23266 a        12442 c        15206 g        20166 t
ORIGIN      
        1 tttgtctcag tccctttttt tgcatatttt tagcatttat ttcctaaaaa atgtaagggg
       61 attcctatat ttttaagtat acttgttttt ccttttatac attttatggt attatcattt
      121 tattagttat tataaaacaa tcgttagaaa ggacgtcatt atgcaagtat acggaattga
      181 tgcggtgctt cgaatcgtga gtcatttggc gtttatctac ttagccttct ggtctttacg
      241 ttcgttacgc attgaaactt ttttcagacc aatgcataat acgcaaatta gaatggcaat
      301 cgtacttttt tcaatttttt tagggtacac tgcaagtaat tttttcttag aagttatcgc
      361 tttatgccga aatcttttcg ttagcctgta aattttaaaa aaaggtacta ttttaaaagc
      421 aaggatgtta taatagaaaa gattttgagt aagtgtatgc tcattggaat tttttggagg
      481 gaacaacatg gaagagatca tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat
      541 tgaaggagct aaaaatgctg ttttgcctat tttagctgct agtttgttag cagaagaagg
      601 tattacgaca ttagacaacg ttccgattct ttctgatgtc ttcactatga atcaagtgat
      661 tcgtcactta aacgtagatg tagattttga tgaacaaaag aatcaagtaa caatcgatgc
      721 ttcaagacaa ttagaaatcg aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat
      781 cgttgtgatg ggtccgctac ttgccagaaa tggacatgca aaagtagcta tgcctggtgg
      841 atgtgcgatt ggtaaacgac caatcgattt gcatttaaaa ggatttcaag ctttaggagc
      901 aaaaattatt caaaaaaacg gctatatcga agcaattgcg gatgaattga tcggtaacac
      961 gatttattta gatttcccta gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa
     1021 agcaaaagga acaaccatca ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc
     1081 aaacattttg aataaaatgg gcgctcaagt ctatggagct ggtactgaaa caatgcgtat
     1141 tgaaggcgtg gatcatttac atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc
     1201 aggtaccttt atggttgctg ctgcaatgac acaaggcaat gtgctgattg cagatgctat
     1261 ctctgaacat aaccgcccat tgatttctaa attgatcgaa atgggtgcag aaatcattga
     1321 agaagaaggc ggagtccgtg tcatcggacc aaaacatatc ttacctacag atgtaaaaac
     1381 gatgcctcat cctggtttcc ctacagatat gcaagctcaa atgacagcta tccaattagt
     1441 tgcagaaggt acaagtgtag tgactgaaac agtgtttgaa aatcgtttcc aacatttaga
     1501 agagatgcgc cgtatgaacg ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg
     1561 aaaccatgaa ttgcaaggtg cagaagtata tgcaacagac ttgcgggcag cagcagcatt
     1621 agttttagct ggcttgaaag caaatggaat cacacgtgtg cgtaacttga attatctaga
     1681 ccgtggatat tataatttcc atatcaaact acagcaatta ggtgcagacg ttgaacgtgt
     1741 agacatggat caaacttctg ctgaaaaaac agcacaaacg attgcttaag gaagtggctg
     1801 aatgagtaac caacgatata tcttattaac tttgataaag attttagtgg tgatcttatt
     1861 gcttatcctt ctttttgtgg ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa
     1921 tccatttaaa gtattccagc caagtttgtg gattcatatt cgagactttt ttcattgata
     1981 aataagaatc acagtggttc tagtcgatat gaagtgagcc atgaagcaga acatgacaaa
     2041 agaaaacaga agggaacgtt gacgttagtc gcgatccctt tttttagtaa aagaaaagaa
     2101 ggaatctaat tgatgaagaa gatattgatt tatttagtta gaggatatca gcgctttatt
     2161 tcgcctgcat ttccacctag ctgccgttat tacccaacct gttccaatta tatgataaaa
     2221 gcaattcaaa cccatggtgc actaaaaggg acggtaatgg ggactgcacg gatcttacgc
     2281 tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat tgacattcca attaacgcga
     2341 aataagaaag ataaaaaaga accagattat aaaaaggttg tgaaaaatac gagtagcact
     2401 gagcgataaa gaaacaattt ggaaaatagc ttcttatatt ttgcaaaatt gtgagttatt
     2461 gccgaagtgt tggattttga acaccgtcaa aaaaaggttg tgaaaaatac gggtagcact
     2521 gagcgataaa gaaaaagagg aaaattattg tcaaagtaac aactccgcaa ctacttatat
     2581 gggggacaaa aaatgatcga agaactaaac aagaaagatt taccatggtc cttgttatta
     2641 gatgcagatc ctgataaaga aaaagttcaa gtatatgttt ccagagggag tggctggata
     2701 tggaaagaaa aagaaaaaac aataggtgta ctgatctatg tggcaaggga aaaagaatac
     2761 gaaatcgtca atgtagcagt agccccaacg tatcaaggaa aggggattgg cggaaaatta
     2821 cttgagacag cttttcagaa gctttcacaa ctcgcttcca gtcaaactca gatcatcata
     2881 cgaaccggca gtactagcag tgcggcgtta catctctatc aaaaaatggg ctatgtggag
     2941 attggtcgag tcaaagacta ttttatccat cattactctg agcccatttt tgaaaatgga
     3001 gatcaattaa gggatcaagt aattttagca attcacttac ctaaaaatag ctttaactaa
     3061 taaactttca attcgagaaa atgctttcaa atgctaaata gatacattac aatagcttta
     3121 gcaaacaaaa aggagaatat ttttatggga atattagttt ttgattttgg tggatcggct
     3181 gtcaaatatg gttgttggga tggcaaagag attaaaggta agggacaatt tgcaacgcct
     3241 gaatcttggg aagaaatgaa agcgcaatta tttcaagttt ataaaaaaat actcgtttct
     3301 tttgaagtcg aaggaattgg aatcagctct cctggagtag tgaataatga gaaacaggtg
     3361 attgagggaa tcagtgcgat tccttatatt catggattta atattttccg agatttggaa
     3421 actctgtttc aactcccagt aacgattgaa aatgacgcca actgtgccgg aatggctgaa
     3481 ttttatgaag gtgctgcccg tgattatcaa gatgttgctt ttgttgtggc aggaactggg
     3541 attggcgggg cgttgttcac gaaaggacaa atcaataaag gggcacatct ttatggtgga
     3601 gaatttgggt tgatgttctt aaatggcgat aagacattca gtaaacttgg tacagcggta
     3661 cagatggctt ggcgttattg tgaacgcaaa ggtctcgata aaaattctta taccggaaaa
     3721 gatgtctttg aattagcaga aactggagat gcaatagcaa aagaagaagt tgagagtttt
     3781 tatacgtatc ttacaaaggg attattcagt attcaatttt cgtttgatcc agaagtgatc
     3841 gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg atgagatcaa aagccgaatg
     3901 aagaagctga cagaacaatt cgatctgcat gatttcgaac cgcagatttt attatgtgaa
     3961 tacagaaacg atgctaatct tgtaggtgca gcagcaaact atctcgctgt acgtcaaggg
     4021 gagaattaag agctttagag aaggtgcagc ttgcataaaa gaagtgaatg aaatgaatag
     4081 gagattttta cctaaggaat gttttatttg ctgcagtatg ataaaatatc tgagaggtga
     4141 aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat agaagaagtg aaaaaagata
     4201 tcaaaggtaa gaattatgaa gtcactcagt tatttatagg gaaaaaaatg attggtgaga
     4261 tcttagccta tgggccaaaa gaatatgaaa ttttctttgg tgaagaagat tttggaaaag
     4321 aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca ctggaatctg cacgaataaa
     4381 aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc tggaaaatta ggtattataa
     4441 ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa gtggctaaac gcgacggact
     4501 gtaaatccgt tccttagggt tcagtggttc gaatccactc tcctccattt cattggggta
     4561 tagccaagcg gtaaggcaag ggactttgac tccctcatgc gttggttcga atccagctac
     4621 cccagtatac cacaatcgta agctctcgtt agttcgagag ctcttttttt tgctttgcta
     4681 ccttgttcct tgaagattct gctgatttaa gatagagtta agttaactac aagaattgga
     4741 gggatccaca tgaaatggaa agaaaaagta gacgaagcag ctgaaaagtt atatgatttg
     4801 gtcaaatcag aaaaatacaa tattgaagtg aatattccga aaaaaggcgg aaaagcggtt
     4861 caggtaaaat caaaacgccc aacgaatcat acaaaaaaat ggatggctaa aaaccgctaa
     4921 taataacaaa agatccataa gtcggcattt tcatcgattt tagggtcttt tgttattttc
     4981 taggttgaac atatctaaaa aattgtttct gacaatagga gtaggaagaa cgatgtgtgt
     5041 ctaaatcctc tgttgatgga tagaaacctt tatctttttc agttgtgaat caggtatata
     5101 ccagttataa tagaagaaac gaaaggacgg gtgtacgtta tgtcgaatca attacctgta
     5161 tatatacaga ttcatgacca attaaaaaat gaaatcgaac aaggtatatg gaaagtcgga
     5221 gaccgtcttc cttcagaaag agaattagca gtgaaattca atgtcagccg tatgacgttg
     5281 agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag aaagaaagat cggttcgggt
     5341 acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg gtgcaacaag ttttacagaa
     5401 attatggaat cgcaaaatcg agtaccgtct agtcggacaa tctcttattt cttgacttcg
     5461 ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag atgacacgat cttgcgaatg
     5521 gaacgtattc gtttcgcaga tgatgtccct atttgttttg aagttgccag tgtgccacaa
     5581 aagctgatcg atggctacag caaagcagag attactgaat cgttgtatcg cacgctagaa
     5641 gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga ttactgctgt gttggcttct
     5701 gagaaaattg ctgactattt agaggtaaaa cgaggagatg cagttcttcg tttgcgccag
     5761 atttcttatt ttgaagacgg tacgccgttt gagtatgtgc gtacgcaata cgtaggaaat
     5821 cgttttgagt tttatttgga aaaataaaaa acaggtgatc cctgattcct tacttagaaa
     5881 taaaagagaa tcagaatcac ctgtttttat atatgatcag ttaccagttg ctcgtgataa
     5941 gaaacaggac cctcttcaaa ttggacttgt ccgttcagca gctcaatgat ggattcttta
     6001 aagtggtcag cacttttttc atctacagca cagatgacgc taacttgatc agtatattgg
     6061 atttctttta taaaaaaatg ttggagttca agataattct gtaattttcc tagttgcgga
     6121 tacgtgatca tcacagagat ctcctgttgc aaggtacctt ctacgattcc aatgtcagac
     6181 aatgcttgag agacagcatg agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt
     6241 cctccaaaat atcgagtgac tactgcagct acattgatca gttgattttt tttgaggact
     6301 tccaacatcg gtacgccagc cgttccactt ggttctccat catcgctgct tcgctggatt
     6361 tcattttttt ctccaatgat gaaggcgctg cagttatggt tcgctttcca atgttctttt
     6421 tttatttctt gaatgaaact ttttgcttgg tcctctgatt ctattcgtct aagagaacag
     6481 ataaagcgtg attttttgat ctcaatctca ctttgtccat tttcttttat tgtacgatag
     6541 cgctcgatca tcttgtcacc tctttttttc attttcttta ttcagtataa ataaagttct
     6601 aggttgtttc aacactgtaa cggttgcgca atcgctgtag tatgcgataa taaagacaaa
     6661 ggtaggtgga tctggggatg caggaacgaa tgaaaaagta cgatgcaatt acgcattatt
     6721 tgaagaacaa tggcggctct caagtaacgt tgacctttac ccaatttgac gaactgctgt
     6781 ttcccagtaa cggacttccc aaaacagcaa gggaaagtac agattggtgg gcaaatgatt
     6841 ataaacaccc tgaaaaaggt gcctatggct ggataaatgc aggctatgaa gtagttgtca
     6901 tcaatttgga caaagaatat gtcgtgttca ataaattagt gaaatctagt tggctatttg
     6961 attaaataaa ccgcgtttag aaaacgttga caaatttctt tctttttata taatagagta
     7021 acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa ggagagaaaa gtatggctaa
     7081 aaaaacaatc atgcttgtat gttcggctgg tatgagcaca agtttactag taacaaaaat
     7141 gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt ttcgctgtgt ccgcttctga
     7201 cgcagataac aatcttgcaa ataaagatgt agatgtactg ttattaggac ctcaagttcg
     7261 ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa ggcattcctt tagacgtcat
     7321 caatatggct gattatggta tgatgaatgg cgaaaaagtg ttagatcaag caatcacgct
     7381 cataggtgaa taaatagaac agtataacta tcgaacaagc gatccaaaaa atagatcgct
     7441 tgttttttcg tatatttttt tagggcgaat aatatgaatc atttttgggg tagaaggctt
     7501 ctgacgagag atattaaagc aatagactgt gaggaggtaa atttaccaaa ttatgagaag
     7561 atcgcagcag tggaacgttt gggcaactgg attctttgtc atcgctgtgg aatgaggacg
     7621 tccgtcattg atggacagct tgcagattac ggttattttt gtacacattg tctatattta
     7681 gggcgatgcg acactcaaca agatttatat ttgtttgatc agcctgaagc aaaacctcga
     7741 gaagtcgtct ttttttggac aggacagctt actgaaaaac aaacggaaat cgcaaagaga
     7801 atactttgcc attcagaaaa aagacatcat cttatttggg ctgtaacagg tgcagggaaa
     7861 acagagatgc tttatcctat tttagtggaa actttaaaag ctggaggaag agtagcgatt
     7921 tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca
     7981 gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg cttatacctt tacttctttt
     8041 gttatctgta cgattcatca gctataccgt ttttatcggt cctttgattt aatagtgatc
     8101 gatgagatcg atgcgtttcc ttataacggg gatgcaggat tagctcatgc tgtgcaaaca
     8161 gcgattaaac cagacggacg attcatctac ttaagtgcca cacctgataa aaagttacta
     8221 aaggaaatca agcagacgtt tgacatccac aaacttgctt tgcgctttca tcggcgttta
     8281 cttcctgaac ccgtcttggt tttcatgaat aattggcgac aaaaatgcaa gaacaagaag
     8341 aagataaaat ctttggtgcg gatcattcat caattaatta aagaaaacca tatcctttta
     8401 ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca cacttcaaga ggctcttccg
     8461 agatatgaga tcacagatgt ttcttcaaga gacaatcagc gggcggaaaa agtccagaaa
     8521 atgcgagaag caaaatatga catactgtta accacgatga tccttgaacg tggagtcaca
     8581 tttgaaagaa tatccgttat cgttttagga gcggatcatc cggtttttac taaatcttct
     8641 ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt atacgaacag tcaagtatac
     8701 tttttctatg aagaaaaaac tgcagctatt cgaaaagctt gtcaggaaat caaagaaatg
     8761 aatgaagaag ggaagaagat gctgtgaggt gcagttgctg cagtaaagat atcgtccaaa
     8821 atttaacatt cagcgagatt attggtgttt cgaaatacag attacctcag acttgttcag
     8881 tttgtcgcgc acaatttcat ttattggaaa caagaggctg ttctggctgt caaaaaccaa
     8941 tccatttcaa tctagagagt acgaaaaaga aagtaaagtc tattcccgtt tactgcagtg
     9001 actgtcaaga atggaagaag aaatatccga cctatgattt tcagcaccgt gccttttttg
     9061 tgtacgatga aaatatgaag gcctggctca atcaatacaa gtttatggga gatatccgac
     9121 tagcaggcac ctttgcatat aaatggacgc agataaaaaa agaatacccg acatacatct
     9181 attgtccaat acctttatct gaaaaaaggc gaacagaaag aggattcaat caagtaagcg
     9241 agatgttgaa agcagcaaga attccttttc agatgctact caaaagagag aagcatctgc
     9301 tggctcaagc aaaaaacacg cgaaaggaaa gattggagat gcctcaacct tttgaacttg
     9361 cagtcacctt ttctaagata caaaataaaa aaatattgat tattgatgat gtctatacaa
     9421 caggacagac tcttttccac gcagcagatt gtttgcaggt cgcacagccg aaaatcatcc
     9481 gaaccatttc tttagcaaga tgaattagat gtggaaattt gatagcgatt taatttgcaa
     9541 ataacctaga gttcgatata atagaattaa gaagagggaa agagtggtcc ttctccttct
     9601 tgattgtggt agacgaaagg ggtaattttt atgtttagat ataatgtacg cggcgaaaat
     9661 atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa aagtaggcaa actagaacgc
     9721 tatttcagtg acgcacccga tgcaacggcg catgtgaact taaaagttta tacagaaaaa
     9781 acagcaaaag tggaagtaac gatcccatta ccttatcttg tattacgtgc cgaagaaact
     9841 tctccagatt tatacgcaag tatcgatttg gttgttgaca aattggaacg ccaaatccgc
     9901 aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct
     9961 actgctttat tcaatgaaga ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt
    10021 acgaaacgcc tttcattgaa accaatggat agtgaagaag cagtcttaca aatgaacatg
    10081 ctgggacata acttctttat ttttgaagat gcagaaacaa atggtacaag tatcgtttac
    10141 cgccgtaaag atggaaaata cggactgatt gaaacagact gatcagcctt acttaaataa
    10201 caacgaaaac ttgcagcagc tactgacagc tgccgcaagt ttttttgagt ttcctcctct
    10261 tttagatgaa atatgaataa attatgaagt tcttacttta atacgcttaa gatatttcaa
    10321 ttatctataa ctaatgataa aatgaaaagt aaagtctaca tagtctggat aatacacaga
    10381 aggaaaagga gattacacaa cgcatggcca atttgattcg ttcacttatc gaaaacgata
    10441 agaaagaact aaaacgttta gatagcattg caaaaaaagt tgaagcatac gctgaccaaa
    10501 tggcagcatt aactgatgaa caattacagg caaaaactgc tgaattcaag ggcagatacc
    10561 aaaaaggaga aacattagat caattacttc cggaagcttt tgcagtcgtc cgagaagcag
    10621 caaaacgtgt attggggtta tatccacacc acgtacaatt gatgggtgga atcgtgcttc
    10681 acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc
    10741 cagtatatct aaatgctctg actggcgaag gggtacacgt agttaccgtc aatgaatatt
    10801 tagcaacccg tgactcgacg gaaatgggcg aactttataa tttcttagga ttaacagtgg
    10861 ggttgaatat caactcaaaa tcttcggatg aaaaacgcga ggcttataat tgtgatatca
    10921 cttatagtac gaataatgaa ttaggatttg actacttgag agacaatatg gtcgtttacc
    10981 gccatcaaat ggtacaacgt ccgctgaatt atgccattgt cgatgaagtt gactcaatct
    11041 tgattgatga agcgagaacg ccgctgatca tttcagggca agcagaaaaa tccacagctt
    11101 tatatacaag agcagacaac ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg
    11161 atgtacaatc aaaaacaatc agtttaaccg aagcagggat cgaaaaagca gaagaaacat
    11221 tcggtttgga taacttatac gacattgaaa atactgcatt gacccatcac ttggatcaag
    11281 cattgagagc caactatatc atgcttttag atatcgatta tgttgtccaa gacggaaaag
    11341 tactgattgt tgaccagttc actggtcgga tcatggatgg acgccgttat tcagatggac
    11401 ttcaccaagc aatcgaagct aaagaaggcg tagagatcga agacgaaacg aaaaccatgg
    11461 cgacgatcac tttccagaac tatttccgta tgtacaagaa acttgcggga atgactggta
    11521 cagctaaaac agaagaagaa gaattcagag aaatttataa catccaagta taccagattc
    11581 cgacaaatcg tccggttatc cgtgatgatc gtccagattt gctttaccca acattacaaa
    11641 gcaagttcca cgctgtcgtg caagatatca aagatcgcta ccgcaaaggt caacctgtgt
    11701 tagtcggaac tgttgcggtt gaaacatctg agttattatc agatatgctg aatcaagaac
    11761 gtattcctca tgaagtgctg aatgcgaaaa atcactttaa agaagcagag atcatcatga
    11821 atgctggtca aaaaggtgct gtgacgattg ctaccaacat ggctggtcgg ggaacagata
    11881 tcaagttagg acttggggta agagaacttg gtggtctagc agttatcggg acagaacgac
    11941 atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg
    12001 gtatgtcaca gttctacttg tcattggaag atgatttgat gaaacgattc ggttcagaac
    12061 gaatcaaatc tttcttagat cggatgaaga tcggtgagga agatgctgtt atccaaagca
    12121 agatgctgac gaaacaagta gaatctgcac aaaaacgtgt agaaggaaat aactatgaca
    12181 cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga acaacgtgaa gtaatctata
    12241 aacagcgaca agaagtaatc atggaagaaa aaagcttatc caagccacta atgaacatgg
    12301 tcaaacggac aatcagtcgt gtagtagatg cgcatacaca attagagaaa gaaaattgga
    12361 atttggaaag tatcatcgat tttgccggca gtactcttgt ccatgaagat agtattactc
    12421 ttgcagatat cgaaggaaaa acagctcagg agatcaaaga gtatctaaac gaacgagcac
    12481 aagaagtatt taataccaaa gcggctcaac taaatggtcc agagcaactg ttggaatttg
    12541 aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac agaccatatt gatgcaatgg
    12601 atcaattacg tcagtcaatc ggcttacgtg cttatggaca gaacaatcca ttagtcgaat
    12661 atcaaacaga aggctataag atgtttgaag atatggtagg cgccatcgag tatgaagtca
    12721 ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag
    12781 gaacagcttc tcactcagaa gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa
    12841 aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga
    12901 aaaaatataa gaactgtcat ggaaaaaatg caaactaatt tctgacagca ggattggtga
    12961 gacaaattct ttttttaaga attcgtctca ccttctttct gttatttgct gattctgtta
    13021 gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gccatgcaag aagaagcaga aacacgtatc aatgcgctag
    13381 atgaaaaaat gaagacatat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt tgaattgcat ccaggggctg gcggaacgga gtcccaagat tggggcagta
    13501 tgcttttacg tatgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggtgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc gaaacgacgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa aatcgatacg taccgagcaa
    13801 gtggtgcagg tggtcagcac atcaataaaa cagaatctgc tgtacggatc actcacttgc
    13861 ctacaggaac agtagtagct agtcaggcac aacgttcaca attgaaaaac cgtgagcaag
    13921 cgatggggat gttaaaagct aagctttatc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact tcgaggagaa caaaaagaaa tcggctgggg ttcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagcagttat ggacggtgac cttgatttat ttattgacgg ctatttaaaa atgcatctac
    14161 aataagaacc cattgttaaa ttgaaataaa attgtaagat tttgcaagca tgttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaatgg tacgaccgct atccgcaatc tctcaattgt
    14341 catcgaacaa ggagagtttg tctatgtggt tggtccgtct ggagccggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gcaatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgctat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggacct
    14641 tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa ttatccggtg gggaacagca
    14701 acgtgtctct attgctcggg caatagtgaa tacgcctaaa gtacttatcg cggatgaacc
    14761 gacaggaaat ctggatccag aaaactcatg ggaaatcatg aaattattag atcggatcaa
    14821 tgcccaagga actactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 gcaccgagta attgctatcg aaaacggccg cattattcga gatcaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagtt gcttcagtca gtgcggtaac gatcacattg actttagtcg
    15061 gtgtattcat ggcagttatc atgaacgcaa ctaaattagc tcaagatata gaaggaaacg
    15121 ttgatgtctc tgtattcgta gatatcggta caaaagacga agagatgaaa aagctagaaa
    15181 aagaattgaa tgacttagat catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat
    15241 tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt gtttgaaggt gacaataacc
    15301 cgttatatga tgtgtatgta gtaagtgcta ctgatccttc ttatacaaaa caagtagcag
    15361 aagaagcagc agatttgaag aatgtttctc gttctgatta cggtggagct tcctcagatc
    15421 ggattttcca aatcgctggt gcagttcgga catggggatt agttgcagcg gcattgctgc
    15481 tgttcgtagc gatgtttctc atttcgaaca cgattcggat cacgattttg tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg atatattcgc tggccgtttt
    15601 tccttgaagg tggctggatc ggtctgatcg gtgcgattct tccgattttg atcatcacat
    15661 ttggttatgc atggtttttc caattgatca atccatcatt attacgttcc cattattcat
    15721 tgattcatcc acaaaatatc gtttggaaaa tcaatctttt aatggtcggc attggtgtga
    15781 tcattggttc acttggctcg atcatttcta tgagacgttt cttgaaattc taataccaca
    15841 atataaataa aatgagaaag ttggacagtt tcgttccagc tttctttttt tattggctag
    15901 gagaatagaa ggaagcggct tttttccctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa gaagaggtga agccgtgaaa attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatc atcgtttttc gagaaagaaa ggaaactgct
    16081 caaacatggg catgggtgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga acctggaaat tgaagaagac aaacaggcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgctcac ggtatttgaa
    16321 agtacgctct atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga ttgaagatat caagcaggca gaagaccata tccacttcca gtattatatt
    16441 tatcgcagtg atgcattagg tgaagaagtt cgagatgcat tgacagaggc ggcaagacga
    16501 ggggtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttccagcttt
    16561 tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt tctttcctct atttgttccg
    16621 tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa tcgtagttat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aatgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tttgcgtatt tacggagaag cggtgtatac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggctattc tcccaaattt
    16861 tttccgtcca ttcactctac tggtgatata gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg aacaaatcaa aatgacttat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgcctta ttacataccg gatggatcga ttcatgaagc actgaagttg
    17041 gctcttcttt caggcgtaaa agttcatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctaatcg agtatggagc aaggatcgaa
    17161 acatatgaga atggatttat tcatgcaaag acgatgatta tcgatggtgg cattgtttca
    17221 gttggctcag caaatatcga tgttcgttca tttcgcttgg actttgaggt caatacactg
    17281 gtttatgatg aacgaatggc ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt
    17341 tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa
    17401 ggattagcaa gattgatttc gccgctcctt taaaacataa aaaggacatg agaaccatca
    17461 acagatggag atctcgtgtc cttttctaac ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagtt ttttttacaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gataaaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact taaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta cggctgttgg ctcttctgct
    17881 ttacagcctt tagtagaaac agttgctgaa acttaccaga cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact ggtttgtctc aagtacagtc aggagcagtc
    18001 gacatcggaa actctgattt gtttgcggaa gaaaaatcgg ggatcgatgc ctcagcatta
    18061 gtcgatcatc gagtagcagt tgttgggatc actcctattg tgaacaagga tattggcgta
    18121 aaagacatca gtatggaaaa cctgaaaaaa ctatttacag gcgagatcaa aaactggagt
    18181 gaagtcggtg gaaaagatca gccagtagtt atattgaacc gcgcttcagg aagcggaacc
    18241 cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccagatcgtt gccgacacac ccggtgcaat cagctatgtt
    18361 gctttttctt atgtttccga tgatgtagat acgttgaatg ttaacggtgt agaaccgaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gatcatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataacatcg ttggagaact aggttatatc cctgtttctc atatgaaagt cgaacgtgat
    18601 tggcaaggaa atatcgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg tggaaaattc atcagttttt tatgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgta gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatcgga
    18961 gcaaatggga aaccaatggt aggggcattg cctatgatca caggttcgtt tattgtgacc
    19021 tttttatctg ctatcgttgc cacgccattt gccattggtg cagcagtatt catgacggag
    19081 atctctccta aaaacggaac gaagatctta cagccggtca tcgagctgct tgtaggtatt
    19141 ccttctgtcg tttatggttt tatcggttta tcagttatcg tgccttttgt cagaagtatt
    19201 tttggcggga ctgggtttgg tatcttagct ggtacatttg tgttgttcgt tatgatcttg
    19261 ccgactgtca caacgatgac tgttgatgca ttaaaggcag tgccacggca ctatcgggaa
    19321 gcttcattag cgcttggtgc cacaagatgg caaacaatct acaaagtcgt gctacgagca
    19381 gccgtgcccg ggattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggtgaagcc
    19441 ttagctatcc aaatggtcat cggtaatgca gcattgatgc caacaggtct tactactcct
    19501 gcttctacat tgacgtccat cttaacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ttatatatag tttcggggat catcgtattg attttagcag
    19741 ctttactact ttatatctta gttcggggaa taccgcacat ttcatggtcc ttcttgaccg
    19801 aaccttccaa agcctaccaa gaaggcggtg gaatcgggat ccagctattt aactcgattt
    19861 atttgctttt gatcacgatg atcatcagtt ttcctatttc tttaggtgcg ggtatctatc
    19921 tttcagaata tgctggaaaa aactggctaa cagatgtgat tcgcacgtcg attgaaatct
    19981 tgagctcgtt gccttctgtc gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcgatt atttctggtg cattggcttt gactgtattc aacttgcctc
    20101 ttttgactag aacagttgag gagtctttac aagcggtcca ttacacacaa cgagaagcag
    20161 gattggcctt aggtttgtca cgctgggaaa cagtgataaa agtggtggtt ccagaagcga
    20221 caccagggat tttgactggt gtgatcctaa gttctggaag aatttttggt gaagcagctg
    20281 cattgatcta tacggctgga caaagtgcac ctgcgttaga ttttacgaac tggaatccat
    20341 taagtgtttc aagcccgatc agcattttcc gtcaagctga aacattagct gtccatattt
    20401 ggaagatcaa tacagaaggc acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat tattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagcaaa atgaaaaaat ataactggaa
    20581 tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta gcactatata cggatgatct
    20641 acatgtctgg tatggcgaca atgaagcaat caaaggagtg gatctggaat ttgaaaaaaa
    20701 taagatcact gcactgattg gtccttccgg ctgcggaaaa tctacgtatc ttcgttcgtt
    20761 gaatcgaatg aatgatggga tcgctaatac gaaaatcact gggaaaatca tgtatcaagg
    20821 cgtagatatt aatgcgcctc aagtggatgt ttatgagatg cgcaaacgga ttggcatggt
    20881 ttttcaacgt cctaatccgt tcagcaagtc gatttatgac aatattacat ttgctttgaa
    20941 acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agctgccttg tgggaccaag taaaagacag cttggacaaa agtgccttgg ctttatctgg
    21061 cgggcaacag caacgtttat gtattgctcg tgcgatcgcg atgaaacctg atatcttgct
    21121 gctagacgag ccggcaagtg ctttggaccc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact atacgatcat catcgtgacg cataacatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tttaggaaaa gtaatggaat atgacaaaac
    21301 taacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agagattatt tcatctaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaagg gatcgatcta agcttcaatc aaggggaaat
    21481 cactgcgatg atcggtcctt ctggttgcgg aaaatccact tatctgcgtt ctttgaaccg
    21541 gatgaatgac ctgattccga atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccaaaaacag atgttgttga actaagaaaa gaaatcggca tggtattcca
    21661 gcagccaaac ccatttcctt tttctatcta tgaaaatgtc atctatggat tgaaattaaa
    21721 aggggaaaaa gataaaaaag tccttgatca agtagttgaa gaaagcctga aggcagcgtc
    21781 tgtttgggat gatgtgaaag acaaattgca taaaagtgct ttatctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat ccggaaatca tcttattgga
    21901 tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaatg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgccgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag cccaagaagt tatcgaaaac gatataaata tcaataacat
    22321 ggaaacacgc ttggagaaga aaagttttga aatgatcgca ttgcaacagc cagttacaac
    22381 tgatttgcgt atgatcatta cggtcatgaa agctagctcg gatttagaac gtatggccga
    22441 tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa gggcagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaacaatc aaaaatatgc aggaaaatcc
    22681 tgagacagtc atcagtggaa cagactactt gaatgttgcc agttatctag aacggatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 gttaaatacg aaccacaatg agattttcta aactgaagct gtgattatgc cgtttgttta
    22861 ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt tctgcatcaa gtaataagaa
    22921 cagtcaaaca aaaaatagct cctcatattt ttcgtttggc tggacttatt accgcagatg
    22981 caagcttttg aacaccgttt attcgggaaa gggtttgtga caaaacatgt cacaaaccct
    23041 tttcatcatt tcatagaaag gataaagaat gcaaaaaaaa attagcatca tcactatatt
    23101 ttttatttgt gtgcttactt catgggttat tcagcatcaa gccgctttat atgaagtacc
    23161 tgtcatcgag atagaaaaag tacgaataaa agaggatcat caattgatta caggaaaact
    23221 gatgaacacg gatcaaaaag gagaatcgat ttatttatcg gttccttatc aaaaaaatca
    23281 aatagaaaca gcaaatataa aagcgagaca aatgtatcta gtccaaatgc atggagatga
    23341 atggcagcta gtagcaaaaa aaaatgatgg ctggcttttc ttcttcacaa gtatcttcat
    23401 tggcgccatg ttgcttattg gtggaaaaca gggtctattt gctttaggaa gtctcatcat
    23461 caatatcgtt ttacttttac ttttgttgct catctttact aaaacagaag ggatttctct
    23521 attaaatata tctatcctgt tcgtcgttat agggatgatt attagcatat tggctttaga
    23581 tggatggaat agaagcagta taatcaaaat ttcagctgca gttacgagtg tttttttagc
    23641 attttttatt tgtcttttga caatgaatca ttggaaagac caaggactgc gctatgagga
    23701 actgaattat ctgacacgac cgtatcgttc tgtttttctt tctagcgtac tgattggaac
    23761 agctggagga acactcgata ccgttatcac agtcattgcg acattagaag aattgactaa
    23821 gcgacaacca gaaataacag cgaaacagtt atggctttca ggaaggcaag tgggtcgaga
    23881 tgtgattggc tcgatgacca atatcctttt gttcgtctat atcagtgggt cagttccatc
    23941 gcttctttta tatttaggaa atcaatggtc gttcaaagaa acgatagaac agcatttatc
    24001 attggaattt ttacgcgtca ttgctggcag cttaggaata gtattatcga tccctatctc
    24061 ggtcctcttt tttctgactg taaggaggct gaaacgatga atgtacttat tatacttgtt
    24121 gggattttag caatcagcgt gctacttatt ggcggtacgc aaggtatgta tattctttta
    24181 ggcttattca tcaatttagg gatctttttc ctgttgttat tcggttatca tcagaagtgg
    24241 ccgatactgg cattgtcggt cataggattt ttattgattg ctgtggttat cttatttttt
    24301 atcaacggtt ataatctaaa aatgcgtgca gcttttgtga gtatactgat ctttttattt
    24361 tgtttcctac tattgcttcc tatcactgat tttttagcaa tccaaggttt tacatcggta
    24421 gaactagaag aattatctgg cttagataag actttagcga ttgattttag attacttacg
    24481 agatcgttgc ttctgattag tcttagtggt gctgttttag atgcgagtgt agcaatttct
    24541 agtgggacat tcgaagtata tcaggcaaat ccgcaattat ctttttacca attgcgccat
    24601 tctagtttcg ctattgctaa aaaaatattg gcgtcaacag taatgacact tctttttgca
    24661 ttcatgggaa gttctttagc gttgatcctc tggtttattg atttagacat tccttttcag
    24721 caagtgatca atgaaaaatc atttgttcta gaatatacga tggctattct taccacattg
    24781 tccgcactac ttgttcttcc tttaacctgc gtgactacag catatctctt tacaaaaaag
    24841 aaagaacaac tcaagatgga gtaaaagtta caacttaagt cctatgacat ttttactgaa
    24901 tcagtgcata atatggatgt aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt
    24961 tccatgaaag aaagagaacg tattttagat ttagtgaaaa aaggaatcct ttcgactgaa
    25021 gaaggcttag atttattaga aagcatggca actgaaaaag acgaaaagca aatcaaaaaa
    25081 gaagcagaca aggtaactgc tagtcataaa gaaaaagatc aggcaagtca attgatcgat
    25141 aaacttgaaa acggcgaaga agaaatttct gagccagttg atccaaagga aagagaaaga
    25201 caagatcaag aaaacttaga aaagatctta gatgaactag caactgaagc gaataaaact
    25261 tcggcgcgtc tagatgaagt aaatgctcaa ttcgctgaca ataaggcagc acgcaatgag
    25321 aaacaggaag cattgatgca gctgaataca aaagaagaat taggcgagtt gacagaagaa
    25381 gaattagcac agcgtcagac gttagaagca gaaatcaaag aattagaagc tagtggcgat
    25441 acactgttag aagaacaatt gaaattagaa gcagaattaa aagatatccg taagaatcaa
    25501 tggtctgaga aaaaagaaac gtttactgat aagtttgaat tgccagatga ttggaaagat
    25561 caagcaacag atacactgaa tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta
    25621 ggcaaattct tgaagaaaac attccaaact gtttcagaaa cagtgaatga caacatggaa
    25681 tggaaagatg tcagcttacg tgtccctggg attgcgacaa cgaaatttga gcatgaattt
    25741 tactatgaag ctcctgcagc aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg
    25801 ttaaaaacgt gggacagcaa cgatgtgaaa gttgaagcta agatcaaact ttacggaaaa
    25861 atgggtgctg aaccatttga agcattctct gaacggagcc agattgaggt taatgaagat
    25921 catatctcct tccaaattcc aaataaacgt gtgcgtgcag accttgtttt ctacttacct
    25981 aaacgagtat acgaccacgc agctattaaa cttctaaatg gcaacatcat gattgaaaca
    26041 ttggaagcta aagatattta tacaaaatct acgaatggta acatcatcgt ggaccaatta
    26101 actgcaacga tgctggaagt agaaggtgta aacggcaata tcgatattcg aaacgggaac
    26161 atcttagatt ctattatcga aacagtaaat ggtacagtga catttggtgc gacagcagaa
    26221 aacctttctg tgtctttggt aaatggtgat gtacgtctta cgattaaaga agataatttg
    26281 aaaaaagtag aagcaagttc agtgaacgga aatgtcaaag tcgctttacc agatggcatc
    26341 ggtttggaag gacatgctaa aacaagtctt ggaagtatca acagccgtct gtccaattac
    26401 gaagtagttc gtgagaaaaa agaacggaca aaccaaatgt tgcaattccg tcgtgtttca
    26461 gatggtgaaa tcgcgcaagt gcaactttca acgacaacag gaagtatcta tttaaaagat
    26521 acagataaat aaagaggttg tgaaagagat gttcagcctt gagcaataag aagcgatttt
    26581 gtaaaatagc ttcccctatt ttacaaaatt gtgccttatt gccgatagtc tatctcttga
    26641 acaccgttta ccaagagatt atgaacctgc caattatcgc cgagtcacaa ggaataattt
    26701 cagaaaaagc tcgtcacgtt ttatgaatat ggtaatttgt gaccgagaat tttgcagaaa
    26761 gacaccgatt aagaagaagg ttgtcacaac gattttcttc atttaaaata gtagaacaga
    26821 ggtgagacat gatgaaaaaa aagttgacaa aatctaataa aaatgttgtt ttgacaggga
    26881 ctttagcagg aataggagaa tacatcggga ttgatccgac tgttatccgt gtgctttatg
    26941 tattcctgag cttagttgct tttggaagtc caattatttt atacatttta ttagctttga
    27001 ttatcccatc tgcgcgttct gcacgaacaa actatggaca taacaatgca tattatgaaa
    27061 aaaacaatta tcgtgaacaa actcgtgaac aaacaaaaaa acgcaaagaa gcggaaaaaa
    27121 tcgatgatga cgaatggagt gacttttaat gtcgtacttt caacgtattt tagttaatac
    27181 gttaacattc atatcattgg ctgttctttt tcctgcgatg gtccatgtga gcagtatttg
    27241 gacggccgtt cttgccagct tcgtattgtc tgtgttgaat atgtttgtca aaccgatttt
    27301 gacgatactg tccctgccct ttacattagt cacattggga ttgtttacgt ttgttatcaa
    27361 tgcagcgatt cttaaaatga cctccttttt tgtgggagca gaacatttta gtttttcttc
    27421 attcggtgca gcgttgttag tagctatctt gatgtcgctt gtcaatgtaa ttgtcagcga
    27481 acataacagt agaaaataaa agaaagctgt gccgaactat cggcgcagtt tttttattta
    27541 gaatcaggaa tgcgttttct attacattgg taattaaata aaaacgtttt ttttaattgg
    27601 tagtactttt ggctagacaa tgataaaatg atagagaaca ctctaataga aatgaggtag
    27661 catgaatgtc agaagttgta aaagttagcc aattagtgaa aaaattatct ttggaaatcg
    27721 tgacaggcga cgagaaaagc ttagaccgtg tcatcgtaac tggggacatc tcacgcccag
    27781 gactagagtt gactggttat tttaattatt attcccataa tcgtttacag ctatttggca
    27841 gtaaagaaat cagttttgcg gaacggatga tcccagaaga gcgtttgatc atcatgagaa
    27901 gattatgtag tgaagatatg cccgcattta ttatttcaag aggattacca gctccagaag
    27961 aaatgatcca ggcagcaaat gaaaatggga tagctgtact gcgttctccc atctcaactt
    28021 ctcgtttatt aggagaatta tccagttatt tagatggtcg ccttgcccct cgaacaagtg
    28081 tccacggtgt tttagtggat gtttacggat taggtgtact gattcaaggt gacagtggga
    28141 taggaaaaag tgaaacagcg ttggagttga tcaaacgcgg ccatcgattg atcgcagacg
    28201 atcgagtaga tgtttatcag caagatgaat taacggtgat cggagaaccg cctaaaatcc
    28261 tggaacatct gattgaaatc cgaggaatcg ggatcattga tgtaatgaat ctttttggtg
    28321 cgagtgcggt acgtggctct atgcaagtcc agttggctgt ttatctggaa gcatgggcaa
    28381 aagataagaa atacgaccgt ctgggctctg aagacacgac cgttgaaatc gcggaagtgg
    28441 gcattccaca aataaaaatc cctgtcaaaa caggacgaaa cgttgcaatc atcattgaag
    28501 tagccgcgat gaatttccgc gcacgtacaa tgggctttga cgcaacgaaa acatttgaag
    28561 aaagattaag tcgcttgatt gaagaaaact caggcaacga ctagcaagga gtgacaatat
    28621 gttagcacaa atcaatcgag ttgcttttga gcttgtaggg attcctatct actggtatgc
    28681 attgatcatc gtatcaggta tcattatcgc tatgtggctt agcagcagag aggctgttcg
    28741 ggtaggatta aaagcagatg atgtgacaga ttttatgttg tggggattgc ctatctccat
    28801 tattggcgct cgcttatatt atgttttatt tgatcttccc cagtatatag ctgatccaat
    28861 ccaaatattc aatatccgtt caggaggctt agctatttat ggcggactga ttgcaggcgc
    28921 gatcacgctg tattttttta ctaaatatca ttttatttct ttgtggactt ttttagatat
    28981 cgctgcacca agtgtattat tagcacaggc aatcggtcgt tggggaaatt tcatgaacca
    29041 cgaagcatat ggaccagaca cgacgcgaag cttcttagaa agtctgcatt taccgcaatt
    29101 catcattgat aatatgtata tcgaagggat ctatcgtcaa ccaacatttc tttatgaatc
    29161 actttggagt ttgattggat ttattttgct tttgttttta cgaaagaaac cacacctttt
    29221 gaaaaaaggc gaagtccttt tatgttatct aatgtggtat agtttcgggc gtttctttat
    29281 tgaaggatta cgtacagaca gtctttactt tttaggtgtg atccgtattt ctcaattatt
    29341 atctgctgtg ttatttgtcg gtacattgat tctatttatt tggcgcagaa aaaaaggaaa
    29401 tttaccggat tatgaccgct cattaggaaa aaatcaacaa attatctgat ttttagagcg
    29461 ttaaaaatga aaaaaatttc aggagtggta aaatgaatta tagagtagcg gttttaggac
    29521 caggctcttg gggtactgct ttggcacaag tcctcgccga aaacggacat gacgttcgga
    29581 tttggggaca tcgtgcttca caagtagaag aaatcaacac ccaacacacg aatcaacgtt
    29641 atttaccaga tcattatcta cccgattcca tccaagcatt caccaatatg gaagatgcag
    29701 taaaagacgc agatgcggtg ctattcgtca ttccaacgaa agcaatacgt tctgttgcaa
    29761 aagaactagt gcctaaattg aatacaaagc cagtgatcat ccatgcaagt aaaggattag
    29821 aacagggaac acataaaagg atctctgaca tcttgttaga ggaaatccca gcagaaaaaa
    29881 gaaaagctgt tgttgttctt tctggaccaa gccatgcaga agaagtagca aaacatgaca
    29941 tcactacgat cacagctgca agtacagatg caaaagcagc aaaatatgta caagagctgt
    30001 tcatgaataa ttattttcgg atctatacca atcctgatgt gatcggtgta gagatggggg
    30061 cagctttgaa aaatattatt gctattggtg ctggcgctat tcacggctta gggtttggcg
    30121 ataatgcaaa ggcagcaatc atgacgcgcg gattagctga gatcagccgt ttaggtgtag
    30181 caatgggtgc caacccgctg acctttatcg gattaagcgg aataggcgat ctagtcgtta
    30241 cttgcacaag tgttcattct aggaactggc gcgcaggcaa cttgttagga caagggcagc
    30301 ccctagaaga ggtgctagcc aatatgggaa tggtcgttga aggtgtgaat actacccaag
    30361 cagcgatgga actagcccaa atgatggaaa tcgaaatgcc catcactcaa acaatctatg
    30421 aagtgctgta tgaacaaaaa gaaatcaaaa cagccgcaaa agaaatcatg ttacgtgatg
    30481 gaaaaaaaga aaatgaattc tcattaacaa ttttttgaag gagcgaatgt atatgaaagt
    30541 taaaaaagca gttattccag cagcaggatt aggaacaaga tttttaccag caacaaaagc
    30601 tatggcaaag gaaatgttgc caattgttga taaaccaaca atccagttca tcgttgaaga
    30661 agcgttgaaa tcaggtatcg aagacatctt gatcgttaca ggaaaagcta aacgaccaat
    30721 cgaagatcac tttgattcaa atattgaatt agaaacaaac ctaagtgaaa aaggaaagac
    30781 cgaattattg aaattagtag aagaaacgac agatgtcaac ttgcatttca tccgtcaatc
    30841 tcatccaaag ggtttaggac atgccgttct tcaagcaaga gcctttattg gaaatgaacc
    30901 atttgtcgtt atgctaggcg atgatataat ggaagacgaa gtaccattga cacagcaatt
    30961 aatcgatgat tacgaacgta ctcatgcttc tacgatcgcc gttatgcaag ttcctcatga
    31021 tgaaacttca aaatacggaa ttatcaatcc aggagaagta ttggaagatg ggttatacaa
    31081 tgtcaaaaac tttgttgaaa aaccagatcc aagcaaagca ccaagtgatt tagctattat
    31141 tggacgttac ttattgactc cagaaatttt tgatgtattg tccgagcaac aaccaggagc
    31201 cggaaatgaa atccaactaa ctgacgcaat cgatacattg aataaaacac aacgcgtatt
    31261 tgcccgtaaa ttcacaggta aacgttacga tgtcggtgac aaatttggtt ttatgaaaac
    31321 aagtattgaa tatggtctaa cacatccaga agtaggtgca cctttacgtg agtacatcct
    31381 gtctttaggt aaaacattag aagcagaagg aagcaagatc gatcataatc caaaaggaat
    31441 aacagaaatc aaagaagaaa agaaaaaata accctttttt ctaacgagct tcagctgcta
    31501 attgaataaa aagtagttga agctcgtttt tatttgttta aaaaacacga aaatgctatc
    31561 cttatagaaa agagagacaa gaagggagtt tgatcatgtc aggaggagaa attgcaggat
    31621 tgatcgcagc aatcgccttt gcgatacttg tcgtttttat cgttcgtgtg ttgttgcaag
    31681 tttctaaaac tttagaaaaa gtagatcaga ctgtagcaga agcaaatacg acgatcgaaa
    31741 tcgtcacaaa agacgtagat cttttgtcta gacaagtaga aggcttactg gtcaaaagta
    31801 atgaactgct cttggatatc aatggaaaag tcgcaacgat cgatccattg ttcacagcag
    31861 ttgctgattt aagtgaaagt gtatcagaac ttaatcattc tagtaaaaac atcgcgacga
    31921 aagtcggcgg aatcggtaaa acgacagcta aagcgacggt tgccggaaaa gtcggtggga
    31981 cagcgatgaa attttttaaa aacaaaaaac atactgaaac gacaggagga aaataaaaat
    32041 ggctaaaaaa ggtggatttt tattaggtgc agtagtcgga ggcacagccg cagcaatcgc
    32101 cgcgttattg ttagcaccaa aatctggtaa agaacttcgg gatgaattag ctgcccaagc
    32161 ggatgatttg atggatatgg cttcagatta cacagatatg gcgaaagaaa aatcgactga
    32221 gttaacaggt attgcaaaag aaaaagcgca agatctttct caacaagcag gtgatctagc
    32281 aggtagtgtc aaacaaaaag taagtgaatc agcagatgaa gcaggaagtg aatctgacga
    32341 tatcttgtta aatcttaaag aacaatcagc tgacttatct aatcgtttca aaaaaacggc
    32401 agaagatatg aaaaaacctg cagatgaatt aggaagtatt gctaaagata cttcagaaga
    32461 tatcatgatt gatgtaaaag acgctgctgg taaagcagga gatactgtca atgaaggaat
    32521 gtcagaagca aaaccagttg cagatgactc aaaagaaatg atgaaaatgg caaaagaaga
    32581 tgcgaaagaa gctaaaaaag aaataaaaga tacaatgaca gattcatcta caaatcacta
    32641 aaatcacaaa tttatagaat aaaaaggtgg gagcggacta gctctcacct tttttactct
    32701 tatttttcta aaacaagtaa ttcttttggt gtagtcgtga atggcacaca gccatcgctt
    32761 gtcacatata cacagtcttc gatacgtacc cctacttttt caggaatata gattcctggt
    32821 tcaagagaga agcacatccc ttcttcgatt actaagtcat ttccttctac aagggaaggg
    32881 aattcgtgga cggttgttcc gatcccgtga cctaaacggt gattgaaata ttcgccgtag
    32941 ccatagctgt tgatcacgtt tcgtgcgatt tgatcaagtt cacctgctgt cacacctgga
    33001 cggacggctt cttgtgcggc aagctgtgct tcaagagtga tgttgtagat tttttcttgg
    33061 aagtcggttg gtttttgata agcaattgta cgagtagcat cactgcaata gccattccaa
    33121 acaacgccaa gatcgaataa cacaagttca ttcggtgaaa tcttcgtatt tcctggtgta
    33181 ccatgagggc ttgcagcatt tgctcctgca aggactaatg tgtcgaaaga catttgtgaa
    33241 acaccttgtt ttttcaattg gtattcaatt tctgcaatga tcgcttgttc agtcacgcct
    33301 tctttcactg ctttaaagcc aatctcaaat gcaacatcgg cccatttacc agcttcgatc
    33361 agtttgtctt tttcctctag agttttgatc agttgtagac gttggatgat ggaagttaga
    33421 tcagtgtaag aatccgttcc cgtcaagata tcagagatag catcgaagcg ctcgacagtc
    33481 aaggcgttct tttcaatccc tacattgcca ggctgaccat agcgagcacg aagttctttt
    33541 ccgatgatct cccaagggtt ttcgctgtct aaatagccac ggacatcata cgcccaacta
    33601 ctgttttgag catcttcaac ttccaaagca ggagtgaaaa ggaaaggatc ttgttcaaga
    33661 gggataaaaa gagctagtac acgctcatga ggatcacttt cgtatccgga aaagtaagcg
    33721 atgtggccag ggtcgctgat ataagccaat tcgacttggt gttgtgccat ccattgacgt
    33781 agttcattga tttttacgtt attcatttgg caacctactt tctaaattaa ttttgtattc
    33841 aataattgta ccactgattt attaaaagac caacgttcta aaaactgaaa aaagtaaaaa
    33901 ttttcatgaa aacagagata aaacggttgc aattcttgag attatttgat attctatttt
    33961 agaaatgtaa gacgtatttt cagaaaacat ataaaatatg aataggagaa cacaaatgga
    34021 aaagcaaaca atcactatct atgatgtagc gagagaggca aatgtttcga tggctactgt
    34081 ttcacgtgtc gtaaatggca atccaaacgt taaaccagca acaagaaaaa aagtcttgga
    34141 agttatcgat cgcttggatt atcgtccaaa cgcagttgcg cgtggcttgg caagtaagaa
    34201 aacaacaaca gttggtgtaa tcatccctga tgtcagcaat atgttctttg cttctttagc
    34261 acgtggtatc gatgatgtgg caacaatgta caaatacaat attatcttag caaattcaga
    34321 tggcaacgac caaaaagaag tcaatgtatt gaataactta ctggctaagc aagtggacgg
    34381 cgtgatcttc atgggtcacc atatcacaga cgaaatccgt ggagaatttt cacgttcaaa
    34441 aactcctgtt gtattagctg gctctattga tccagatgaa caagtaggta gcgtaaacat
    34501 tgattatgta tccgcaacaa aagatgcagt aaacaaattg gcaaaaagcg gcaataaaaa
    34561 aatcgctttt gtcagtggtg cgttgatcga tcccatcaat ggacaaaatc gtctgaaagg
    34621 ctataaagaa gcattaagcg aaaataacct accatatagc gaaggattga tctttgaagc
    34681 acaatacaac ttcaaagatg gtctatctct agtcgatcgg atccataata gcggtgcaac
    34741 agctgtctat gtttcagatg atgaattagc catcggtatt ttggatggtt tattagaccg
    34801 tggcgtgaaa gtaccggaag aattcgaaat catcacaagt aacaattcac tactgacaga
    34861 agtagctcgt cctcgtttaa caagtatcac acaaccatta tacgatttag gggcggtatc
    34921 tatgcgccta ttgacgaaaa tgatgaataa agaagaagtc gaagaaaaaa cgattatctt
    34981 gccttatgga atcgaagaaa aaggatctac aaaataataa aaggacaacc attcagaggt
    35041 tgtgtgcctg ccgtttaact ctgcgttaca aggagcaatt tcaaaaaaca gcatctcatg
    35101 tttgatgaat attgtgactc gtgattcgga gttggcagga gaacaccgtt tatttgggaa
    35161 aaggggttat gacaaaattt tgttataatc ctttttttgt gtcttaaaaa agagagaaag
    35221 tggttgaaat tacatttatc gtatataaaa caatccgtgt atagatgctg cattttgttc
    35281 accaccatca aaaaaataag ggactgtgac aatactattg tcacaatccc ttattactgt
    35341 gtattctatt aaatcaatca tctttctcat ctgattcttt agttgcttga ttcgctcttg
    35401 cataagctga agcggtgttc cagtaatctg tatagttttc atcggtaccg ccaacgccaa
    35461 atttaaaggc gcttttcttc ggtccatttt tcgcccatag cgattcaact gtttttcctg
    35521 gtgtttggat ggatttttta ttgacagtaa cttttccagg tgtttgacca gtaaatccag
    35581 atactttttt cttcatcaca tcagatgata attcgaattt ctcatcagtg ccaaaaatgt
    35641 caggttttgc ttggtaagct tgattgatca gattggcaag ataattagcc gaacgtatcc
    35701 ttgctttgct gtccattcca gtattgtcat cgtgccctgc ccagctactg atggtaatag
    35761 acggagtaga gacaattagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    35821 cccaatctgc ttttcctaga tttccgttta agcttgagat ggcatctttg aatggtgtag
    35881 tgatttttgc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttccg
    35941 aatagatccg gacagagttg ctttcgtgtt tataaattac atttcctgcg ttatctgtga
    36001 tggaatcgat gatatagcct tcttcataga cgccgccgtt tgctaaagcc tgaaatccgt
    36061 tggtctgttg aagcgtcgtg acatctactg tacctaaagg agcagattca acgccccaat
    36121 tgtcatttgc cgggtagttc atctttgcca agtagtgctc gtaagcatat tgtttcgatc
    36181 caccggaatt caacacatct tgatataagt gataagccgg gatattgttg gaccattcta
    36241 gtgactcacg gacggtttgg aaggtgttcg atccttcgtt agtagcattg acgattttct
    36301 cgccagcatt ttctccttct tgccaagttg ttgcataatc tgatacacgg gattctgatc
    36361 cgataagccc catgtcgatt gcaggtccat aaacaagaac tggtttgatt gatgagccag
    36421 cttgacgttc tgtatcgaag gcatggttat tttgattctg actgtaattc cgtccgccaa
    36481 cgaatccgta gatgcggcca gttctgttat ccatcaatac atttccggtt tctacttgac
    36541 tgccagcacc atcatctaac atgtatccat aattttcaac gccattttgc aatgcagcat
    36601 agacatcttt atcgattgtc gaatggattg tatatccttt attttggatc gtttgctgtg
    36661 ccttttgata ataagcgtca ctgaccgatg aatcagacat atcagcgttg tctttttcag
    36721 ccaattgttg tgcaatgatt cttgttgctt cattcatcac agtataatac aagaattctc
    36781 tgtcagtctg ttctgcgatt tgttgtggta gaaagtcttt tgtaaggtca taggcttttg
    36841 cttcttcgta ttgttctttc gtaatttggc cttcacgata catactgaat aaaacaaaat
    36901 cttttctggc aaggccggca gaaagatctt cttttagcgc gcctgtgttc gtgtatggac
    36961 tatacgtgat cgggctttgg ggtaatccag cgatataagc agcttgcggc agcgtcacat
    37021 cttttgcatc tacaccaaag attccttttg cagcttcctg gactccagca atgttttgtc
    37081 ccttattatt acgaccaaat ggtgagacat tcaaatacgt agcaatgatc tcatcttttg
    37141 aaaagtattt ttctacttgt gcagaaagaa tgatctcgtt tgctttacgt ttgaatgttg
    37201 tttcatctgt aaggatctgc tgtttgacta attgctgtgt caaagttgat ccaccagaag
    37261 agccaatacc agtagcttca gatactaatg ctcgtaaaac agctttagga acgtaacctt
    37321 ggtgtttatc gaagtattca tcctctgtgc tgataattgc agttttcaat aaaggtgaaa
    37381 tcttatcaga tgaaatagtc gtgcgcatca gatctgtctg gatttttgat atctctgtat
    37441 tatctgcata aacaatcttt gacgtttctg taatgtttcc tagttctgtc tgcagtgctt
    37501 ttttagtcgg caattgtgtg tcttcgacga gataggcaaa atagccagcg cctattccaa
    37561 tacctagagc tgcgaacatc aaaagaagaa cagtaataaa aacagtcaat gattgaaaaa
    37621 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    37681 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    37741 caaataatag aacaaaaaaa cagacaaggc tgtttttggc aggaatttct tttttaaaag
    37801 agttaaaacg tgagaaaatg atctttatta taaaagagtt cttaagcttt tctttagaga
    37861 aaatttcagt tactatggag aacagggcaa agaaaaatca atcattttct ttgtatatca
    37921 tcttaccaat atataggaat tgttagtaac aattgccttt caaggaggaa tccatccatg
    37981 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggagc agcagaagaa
    38041 gcagccagtt atggatcttc gacttttatg atctatacag gcgcccctca aaacactaga
    38101 agaaaagcca tcgaagagat gaatattgat gccggaaaag cgtacatggc agaaaaaggt
    38161 ctaacaaata tcgtcgttca tgccccttat atcattaatt tggggaatac gatcaaacca
    38221 gaaatgtttc cttttgccgt ccaatttctg agagaagaga ttgttcgtgc ggaagcttta
    38281 ggcgctactc aaattacact tcacccaggt gctcatgtag gggcaggaac agaagccgga
    38341 attgctagaa ttattgaagg cttgaatgaa gttttgacaa aagaacagac ggcacaaatc
    38401 gctttggaga caatggcagg taaaggtacg gaaatcggtc gaacatttga ggaattagcg
    38461 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga cacctgtcat
    38521 acgaatgatg ccggttataa tattaaagaa gacttttcag gtgtaatgga agagtttgat
    38581 aagatcatcg ggttagatcg gcttaaagta ctccatatca atgattctaa aaatccgcaa
    38641 ggttcgcata aagaccggca cgccaatatt ggttttggaa caatcggatt cgacacgctg
    38701 aataagatcg tccatgatcc aatgtttgac aatatttcga aaatcttgga aactccctat
    38761 gtaggcaaag ataaaaagaa agcttacgca ccatatggaa aagaaatcgc catgttcaaa
    38821 gcgcaaacat tcgatccaga agtgtttcct gagttattag cagaagaagt gatactctaa
    38881 gattttctat ctgacagcag caaaaaagga ccaggcacat tgccttgtcc ttttttgttt
    38941 aagaaacaaa ggagtacttt ttgcagtctt tttcatcatg catcttctcc cccctctttt
    39001 taaatttaga caaacaaggt atactggtag aaaagtaaaa aaacgaaaag tttcacaagc
    39061 aagtggcaaa acgtgattgg aaggacgaaa agatgctgaa cactttaaaa cccttgatca
    39121 accaactaaa aaagaaacat acatacttta ttcttggcat agccctatta tttacaatta
    39181 ttcgtttgta tttatatagt aaagcaatct atggaacaga catgtcggca acctatgatg
    39241 accagctgct gattcgctat gctcaaaata ttttggacgg taaatggcta ggggagtata
    39301 cgactacaac actttccaaa ggaatctcct acagcttatt tttagtactt gcacataaac
    39361 tgcatctttc ttatgcagtt ttgttcggca tgttcagtat ttttgccagt atattggttg
    39421 cactcgcgtt taaaccgatt gtgaagaaca agtatcttct tttaagtatc tatttttatt
    39481 ttttgttttc tccgattagt tttacacatg aatattccac tcgaacatat cgtaatgccc
    39541 tcgtgattcc ggctgttgag atcattatcg cttgtttatt agctatttat taccgcaaat
    39601 acggttctag cagaaaattg ctgccatggt ttttagggct ttctgtcgct tttcctttct
    39661 tttggttcat tcgcgaagat tctatctggc ttcttccttt tactgtagta gctttgggta
    39721 tcagtttcgt ccagatcttg ctagggcaag acttttcttt caaagaagta aagaagttaa
    39781 aaaaacgact ctttcttctc tcgaaacggg aatggctgaa actttcattg tttttacttc
    39841 ctatagttgg cttttccatc gtctatcaat ggatcgaata taaaaatgag acaacatacg
    39901 gtattgcaac catcaatgac cgttccagtg gagaatttgg acgtttgatg aaacaactga
    39961 tccgaataga tgatgaaacg aacttgaata aagaaaacag tgagatatgg gtctcacatg
    40021 ctgcactaaa taaagcatta gccgcatctc ctacattcca tactgtttct acccgtattg
    40081 acgaattata tacgactcac gcgtggactc aaggtggaaa agtaaaagaa ttacccggag
    40141 atatcatctt ttgggcattg cgagatgtgg taagcgaatc tggttattac caaaacaatg
    40201 caaaagaaac caatgatttt tggaaaaaag tcaaccaaga attagaagaa gcctatcaca
    40261 agggaacaat caaaaagaaa aaagagttct atctgatggg atcaggagat ggaaagacga
    40321 tgagtgatct gccaatcgta ttcgatttcc ttcaatcagg atacgcttat gcatggcgat
    40381 ataaagattt ccatcaagga ggaaattata gtttcggaac gcaagaacaa gtcaaacagg
    40441 ctcaagaatt attaaacctt ccgctaatgg agaattggat cgatagtaat gaaccgagaa
    40501 cacctaattt ccaattaaca aaatcagcaa aagtcgcgaa tgtcatcata aaaatttacc
    40561 aagttctggc tattcccgtg ctgattgctt caatagcagg gatattactt atgatactag
    40621 gaaccgtatt tgcaaaagct gatcgtgcct attatagttc ctctttgatt gtggtcaccg
    40681 gattaatact gacacaattg gtttttcttt tcggtgtctc atggttttgt tcttatgcgc
    40741 cagaaaaaaa ggactatttt ttaagtgtct acactggtgc aggcgtgcca ttaacgcaat
    40801 tcatcgtagt aacaagcatg ctagttttca gtaaattaaa tctgaaaaaa gctttatctt
    40861 ctaaataaaa agacccttgg tagctagatt tttaacctaa ctatcaaggg tctttgtgtg
    40921 gaaaaatcag tttttattcg atttcttcta ccgtttcctg caaaagctgt tcatattttt
    40981 tgtttccttt atataaatca ccaatcgtcc atgccaacag gagaaggaca agaacaatga
    41041 ttacataagc gatgtgatca gccaatgcta gttgagcgga agtggcttgc tcgccgaaga
    41101 aactttcgat ctgtcctggt aatcctcgta agttcaagta agttagacta atcacagaga
    41161 accagccaag aagttttacg atgaatgaat ttttaaatcg gctacccatc tccgcttggc
    41221 tgttggtcat catcagtaaa ggaatcatcg aaaacggcaa cgcaaaagcc aagaacacct
    41281 gggattcatt cattaagtta ttcaaagcgg catgttcttc gatcgttcct tttgtacttg
    41341 taaacaaaac acaaatcaat acaggaatca cagaaagcaa tcgtgtgacc agtcttctta
    41401 gccaaatcgg gatacgcata tggatgaatc cttccatgat gacttgaccg gtcaacgtac
    41461 cagtaatagt agagttctgg ccagaagcaa gaagtgctac agcaaataaa gtggacaaga
    41521 taccagattt tgctacagca atcaaaatac cattgctcat agctgatgga tcagataaag
    41581 cttcatacag accaaaaaag gaaggatctt ggacagaacc ggttttgaag acagcgacac
    41641 ccatgatcaa tagcagtgca ttgactagaa aagccattga caactggata ttagaatccc
    41701 aagtagaaaa acgtacagct tgtgctacat cttcttcgtt ttggtgatcg atcttccgag
    41761 tttgtgaaac tgctgaatga agatataagt tatgcggcat gactgttgca ccaataatcc
    41821 ctaaagctcc agtcagcgga gtttcgccgc cgatgaccgg tgagtctgag aatgtatgac
    41881 tgttagggat aaagccagca aataaactgt gccaatctgg gttagacaag ccaacttgat
    41941 agacaaaaac gaataaaatg acgaaaatta agcagacaac cagtgcttcg atcttacgaa
    42001 acccgacttt tgtcagtagt aagagaacga aaacatctaa tacagtaata aagacggcaa
    42061 tcaccaatgg aatatcaaaa agtaagtaga gggcgattgc tgcaccaatg acttctgcaa
    42121 tatctgttgc cataatggct aattcagtca agatccataa aatgacacct aatgtcttac
    42181 ttgtgcgtgc tcggattgcc tgtgccaaat ccatttggct cacgattcct aattttgctg
    42241 ccatatactg taaaagcata gcaatcagac tagacatcaa aataactgac atcaataaat
    42301 attggaaatt ttgtcctccg gtgatcgaag tagaccagtt acccgggtcc atatacccaa
    42361 ctgctactaa cgcaccaggt ccagagtaag caagaagtgt tttaaaaaaa cttttcccct
    42421 ttggtacatc aactgttcca tttacttcag ctaaagataa accatttgta tgttcaataa
    42481 gtcgatgctt atgaaattgt ttattggatt gattattcaa gaaaaaacca cctttcctat
    42541 ttatctacga ttacattcta cacctcaaaa agaaaaaatt aaagaaaaag attcggtacg
    42601 cctaattttt tttcatggcg cgtcttgcta gatgacgaaa gaaattttgt tctcctctaa
    42661 aaaaaccact attttttgca tgaaactaaa gaatacgcct ttcttttttc aataaacttt
    42721 agtaaaataa gtcattgaga gcaattcgga aatagaaagg agagatttat atgttatcaa
    42781 caggtattgt cgtacttatt gctattattg ctttgctaat cggcgcagta ggtggctttt
    42841 tcctagcgcg taaatatatg aaagactatt tagaaaaaaa tcctcccgtt aatgaggaca
    42901 tgttgcgatc aatgatgatg tcaatggggc aaaaaccatc tgagaagaaa attcgccaga
    42961 tgatgcagca aatgaagaac caagggaaaa aataggttct gacccaacga ctttttacgg
    43021 gctgattttt tcagtccgtt ttttatttcg taatttgtta gactattcag attattcagc
    43081 aaaaaagaca agcacagaca atcctaggct cttgataaag acaaaggagg agtaaaatgt
    43141 cgatttttag aaaattaggc tggttttttc gtcaggaaaa gaaacattat ttaattgggg
    43201 ttttctcgct attcgcagtg gccttggtac agttagttcc tccaaaagtg attgggatca
    43261 tcattgatga gatcgccgat aaaaatttat caataaagat cattttcggg tggatcttcc
    43321 tattgattat cgctgctttt ttacaatatc tttttcgtta tatctggcgg acgaatattt
    43381 ggggaagtgc agctcgattg gagaaagaac ttcggcagca gttattcaaa cattttacag
    43441 aaatggacaa tctcttttac caaaaatatc ggactgggga tttgatggct cacgcaacga
    43501 atgatttgaa tgcgatccag aatgtagcag gagctggtat cttgaccttt gcagattcag
    43561 tgattacggg cggaacaacg atcattgcga tgattttgtt tattgattgg cgcttaacac
    43621 tgattgcgct gattcctctg cctttgctgg ccgtcacttc acgagtgctg gggtcaaaac
    43681 tccatgatgc ttttcgtgat tcgcaagaag ctttttctag tatcaatgat aagacacaag
    43741 aaagtatcac tgggatcaaa gtcattaaaa catttggaca agaaaaagaa gatctagcgg
    43801 attttgaaga aaagattgaa gatgcaatta ttaaaaacaa acgagtcaat tttttagact
    43861 ccttatttga tccattcatt accttgatta tcggtctgtc ttatgttgca acaattattt
    43921 taggtggaaa gtatgtcttg gaaggcacga tctcgatcgg gcagatggtc tcatttatta
    43981 gttatatcgg tatgcttgtt tggccgatgt ttgcgattgg tcgattgttc aacgtattgg
    44041 aaagaggaaa tgcttcttat gatcgcgtca atgaactttt gcatgaaaag acgcatatcg
    44101 tggaacaact gggtgcagtg cagaccccgg caaaaggcga gctgaatgta gctatttcct
    44161 cttttaatta tccgaaagaa gaatcaccag cattagaaca aatcaagttt gcgatccaag
    44221 aaggagaaac attaggtatt gttgggaaaa ctggtgcggg aaaaacgacg atcatcaaat
    44281 tactgatgcg tgaatacgat gattatcaag ggaaaatcca atttggcgga cgttcgatca
    44341 aagaatattc attgaatgct ttattaggat caatcggcta tgtgcctcag gatcacttct
    44401 tgttttcgat gaccattcga gataatatcc gtttttctaa tccaaaactt tcagaagaag
    44461 aagtagagca tgcggcttct ttagcattta tcaacaatga aatcaaggaa ttaccaaaag
    44521 gttatgatac cttagttgga gaacgtggag tctctttatc tggcggacag aaacaaagga
    44581 tttcgattgc tcgagcattg atcaaaaatc ctgaattatt aatattagat gatgcactct
    44641 ctgcagtaga tgccaaaaca gaagaagcta tcttgcataa tttaaaagaa ataagaaaag
    44701 agaaaacaac tatcattgca gctcaccgtt taagtagcgt gatgcatgca aaagaaatca
    44761 ttgttgtcga tcaagggaaa atcattgaac gaggaaccca tcatgagctg cttgcactag
    44821 gcggctggta tgaacaaatg tgggagaaac agcaattaga agcgaagata gaaggaggcg
    44881 aggcctgatg gatgaaaatt atcaatcgga atggacaaaa tcgattccac ttaaagaaca
    44941 gaaaaagatc atcaaacgac tgatgaagtt cgccaaacct tttagaagaa cgttcatcgt
    45001 tgctatctta tttgcgtttg ctttgtctgt gatcaatgtc ctattgccaa ggatcatcca
    45061 aacttttatg gatgatcatc tggctaaaca gtcggctacg acacaagtta ttttgttttt
    45121 tgctggtatg tacttatttg gagtcatagt aaaaagtatc atttggtttt tccaatggta
    45181 tttgtattcc atggcctcat tgaagaccta tcagtatatt agggtaaaat tatttgaaaa
    45241 gctgcataca ctaggtatgc gttattttga tcagacaccg gcaggatcaa ccgtttctcg
    45301 agtgacgaat gacacagaaa cgctatttga attttggtac gtctttttga tggtattgac
    45361 tgggatcttt gcagtcgttt cttcattcct tgccatgttt caaatcaatg gagaaattgc
    45421 cttgtggact ttggtgtttt tgccgatttt aggaatcgtc atctggtatt accagaaatt
    45481 cagttctcgt atttaccgaa atatgcggga aaaattaagt cagctgaaca cgaaattgaa
    45541 tgaatcgatt tccggcatgc gtattatcca gcagtttcgc caagaagctc gtttagctaa
    45601 agaatttgaa caagtgaaca atgagtattt agacacgcgt tatgcgatga tcaaaacgaa
    45661 ttcgattctg ttaagtccga tcatcaattt tctatatgca ttggcgattg cgttggtgtt
    45721 gactttgttt gggatcgatg cgttgcattc tcccgtagaa gttggactga tctatgcatt
    45781 tacgacctat gtccagggat tttttaatcc aatgtctcaa atgatggact ttttgagcat
    45841 cttcacagat gggatggttg ctggtagtcg tatcttgaag attatggata cggaagaata
    45901 tgcaccacaa caaaaccctg gagcagatca aatgattact tcaggtaaga ttgagtttcg
    45961 caatgtaagc ttttcttatg atggagagca caatgtatta aacaatatct cttttgttgc
    46021 taatccggga gagaccgttg cattagtggg acacaccgga agcggaaaaa gctcgatcat
    46081 caacgtattg atgcggtttt acgaatttta tgaaggtcag atattgattg atgatcatga
    46141 cattcgagaa tatccaatgg aagagttacg taagaaaatg gggctggtct tgcaagatgc
    46201 tttcttgttt tatggggata tcgcaggaaa tattcggatg atgaatccag ctatcacaga
    46261 cgaacagata cgccatgcgg ctgagtttgt tcaagcaaac aaattcatcg atgaattacc
    46321 aaaaggatat cactcaaaag tgatcgaaca tggtgccagc tattctagtg gacaacgaca
    46381 gctgatcact tttgcgcgaa caatcgtgac tgatcctaaa attctagtat tggatgaagc
    46441 aacagcaaat atcgatactg aaacagaagg attgatccaa gaaggattgg caaaaatgcg
    46501 tcaaggacgg acaacgattg cgattgccca tcggctgtca acgattcgag atgcagaatt
    46561 gattttagtt ttagacaaag gtcatatagt agaacgcggg aatcatgaaa cacttctagc
    46621 gcaaaatggt ttgtatgcag atatgtacaa actgcaaagc agtggtatat aaaagatgtt
    46681 gtgacagtag aattttgacc taagcactag agtgaaaata aaacatagct tctcatattt
    46741 ttcatctgtg agagttagtg tcgtagtcac cagatttaaa aaaagattcg acagctagtc
    46801 agaagtgcct aatagccact aagaaagcgt tgacaaaagc atttgctttc gataatataa
    46861 agaaaacgat acaacttgga tagtgattgc ctgatgcaag ctaaacctga tgagtctccc
    46921 atggtttcat ggacggcttt ttgggttttt ctttttgttt tggatacagc cctatacatc
    46981 atggaaagga gcaaaacatg ctgattgaga aagtactgaa taacaacgtc gtaataacca
    47041 gggatgaaag aaatcaggaa atgatcgtga tgggaaaagg tttggcattc aaaaagaaag
    47101 tgggtgaccc gatttttgac tcatgcatcg acaaaaaatt tcagttgaca aatgacgggc
    47161 tttcacaaca gttccaagaa ttggcacaag cgattcctct tgagtactta gaaatttctt
    47221 gtgaaatcat tgaatataca aaagaggctc tacaagtcca gttgaatgat tctgtgtata
    47281 ttgcattgac ggatcatttg tatacggcta tcgaacgtca caaaatggga atatttgtac
    47341 caaatgtatt gctgtatgat atcaaacaac tatttcctaa agagtatgcc atcgggaaaa
    47401 aaacagtgaa gaaaatctcg gataaatacc acatcaagtt atcagaagat gaagccggat
    47461 ttatcgcatt gcatttagtc aatgcacaag gcattcaaag ccagcaagac aatacctacc
    47521 tgtttactat aacaatacag gatattctaa atatcgtccg ctattactat aagattacat
    47581 ttgatgaaga ttcagtccat ttttttcgat tcgtcacaca cttacgtttt tttctgacac
    47641 gcgtcctaga tggagaaaaa tcagaagtgg gagaagtttc tgaggaattg tttcaggtta
    47701 ttcaaaaaaa atataaggat acgtttggtt gcgttgagaa aatagcagac tttttagccg
    47761 agacgttcaa ctaccagatg tccacggacg aaaaaatgta tttaacgatc catactgcgc
    47821 gtttagtgca tgagaatcgt tgtaaataaa aaaaggagga taaattaaca agaacgtatg
    47881 aaataagggt agtgacattc agttggctaa accttccact aataaattgg agggaataga
    47941 aatgggaaaa taccaagaat tagcgagaaa aatcgtagaa aatgtcggcg ggaaggaaaa
    48001 tatcaatggt ttgacacatt gtatcacaag gcttcgtttc aaattaaaaa acgaagaaaa
    48061 agcaaatgat gagatcttga aaaatatgga cggtattgta acggtaatgc gagcgggagg
    48121 gcagtatcaa gttgtcattg ggaatcatgt gccagtcgtg tttgaagagg ttataaaagc
    48181 aggaaacctt acttttgatg aggctgtttc aacagaaaaa atgagaccgt ttgatatgct
    48241 tattgatgga atatcgggct gtttccagcc attccttgct atcctagctg ctggcgggat
    48301 gattcgtgga ctgacagcat tccttgtctt cttaggcgcg tttgaccgcg gttctggcac
    48361 atttgttatg tttgataata tcggtgattc tgtattccaa tttatgcctg ttattatcgg
    48421 tttaacagct gcccgtaaat ttaaagtaaa tgaattcgta gggatgttgc ttggcgcagc
    48481 attaatgaat cctagcttgt cgctggaagc tttgagtggt gcagcagaag caccactaac
    48541 gaccatattt tctggcacta tttttgaagc acctatttat caaacagtat tcggcattcc
    48601 ctggattgcg cgtaactacg cttcttctgt cattcccatt atctttattg tgttgttagc
    48661 tagccaaatt cagaaaccaa ttaaaaagtt agtacctgag atgatcgcga atttcttcgt
    48721 tccattcttc accgttctta ttacaatgcc tttaggtttt ttactcgttg gaccagtatt
    48781 cacattcgca actgatattt taatggctgg atttgaaaca cttttagcac tttcgccagt
    48841 tatttatgga gcaatcgtag gcttcttctg gcaaattctg gtgatgtttg gtctccattg
    48901 ggcaatcgta ccaatgggat tgatgcaatt ttctgtgaat ggttggcaaa atattatgac
    48961 acctgttgca gtagtgtctt ttggacaaac tgcagcactt accgcactct actttaagct
    49021 tagaaatcca aaagacaaag caattgcaat accagccatt gtttctggta ttgtggggat
    49081 tacagagcca gctatttatg ggtttacatt accacgtaaa aaaatattta tttttacctg
    49141 tgtcggtggt gcgttagggg gcgcgtatag tgggttaatg aatttaactt cttggaatca
    49201 aggtggacta ggcatcttta caattcccaa ctatattaga ccagatggtg atttaacaga
    49261 tgtgattaat gtattgattg ggatcgccat tgcgatggtg gtatccttta cgctgacttt
    49321 tttcttttgg aaggatgaag caggtgaaac agatgatata cagaaaaaat caggtaaaga
    49381 aattgtaaaa acaccgatac aaggacaaat cgctccacta aatgcagcaa aggatgcagc
    49441 ttttgcacaa ggaacactag gtcgaggaat cttgatctac cctgaaaaag gagaagtacg
    49501 tgcccctttt gacggaacag tcatgaccct gttcccgacg aaacaggcaa ttggaatggt
    49561 ttctgaaaca gggctggaat tgctgattca tgtcggattg gatactgtgc agttggaagg
    49621 gaaatatttt gaaagcttag tgcagcaagg ataaagagtg aaacaaggcg atctgttgct
    49681 acgcttcgac aaaaagaaaa tcgaaaagga aggatacagt ttagaaacac ctgtgatcgt
    49741 caccaattac acggattatt tggatatttt ggaaaaccaa ggggagacgg tcgggccaga
    49801 cgatgcactt atcaccgcac tgacctgaac ggttaggtga agaatggact aggcttaact
    49861 attcttttta ttccacttct ttattttcaa aattgaaagg ctctttcatg atggctgagc
    49921 tgatagggcg tttatcgaat ttttgaccga cagaaattag atggactaaa ttctgttgat
    49981 aattttctgc aaaaacacaa ctatataaca cgtctagaag ataacaaatt gatgtattga
    50041 tcgtaaaatt accaattttt gaatacagtc gctcgcgtgt cgtaagtcgc aaagcggctg
    50101 tactcattgc agatagcgtt gaatcaccga tactggtcaa agctaaaatt ggaacatgat
    50161 tttgtttcaa tatttctgcg gtacgaagga tcacattgtt ttcacccgta taagaaatca
    50221 gaatcccgca catatcactc gtgcaattgt acgcttcata gaatgattca ccaaaagtgg
    50281 tgacaatcgt gacatccatt ttgatgcgct tcattttcag catgaaatct tgagaaatca
    50341 aagtattcga gttactggcg aaaatcacga ttttttttgc ttgaagcatt agttgtttgg
    50401 ctttttgcag ttggtcatga tgaatgagag aaagtgtatc atgaatcgtc atctgttcta
    50461 aagaagccat tttatttgcg atcgtcataa taccgtctgt ggtggaaaaa ggaagattcg
    50521 catctacgtc ttgaaaattc ccttcaagat aggctgtttc agctaaaaaa gcattacgaa
    50581 aactgaccca gccatcaaaa cccatttttt ttgctacacg gatgagtgta gaggggtgga
    50641 cataacatcg ctcagcaatc gcttggatgg tcatttcgcc aagtgctgga gaatcgctta
    50701 agagaaactg gatcacttca ttttctgaag gagaaaagga gatttctttg attttttcac
    50761 gtaataacat gtagatacct tcttcttatt tttttacgta aattataact tttttgaaaa
    50821 tgatttgtaa acggatttat actatataac ttaaaataga cattttttga caagatattt
    50881 acaaaaatcc actagaaaac agtaaatttt gtaaatgtta ctggaaagaa acagatttct
    50941 attgtttgtt ttgtttacaa aaaagataat gaaagtgtaa aaaagtaaag gagtgagaac
    51001 atggcaaaag gaatcaaaat cgctacgatt ggtggaggca gcagttacac ccccgagttg
    51061 atggaaggat ttatcaaaag atatgaggaa ttaccgatac gagaaatctg gctcgtagat
    51121 gtagaagaag gaaaagaaaa gcttgagatc gttagtcaga tgtctcagcg tatgtgggat
    51181 gcttctccgt atgaagtcaa gatctatgca actctggatc gggaagaagc attgaaagat
    51241 gcggattttg ttacgactca gtttcgtgtc ggtcaattag atgcacgaat caaagacgag
    51301 cggatcccta gttattatgg gatgattggg caagaaacaa atggtgctgg cgggatattc
    51361 aaagcttttc gtaccgttcc agtcgttttg gcaatcgttg aggacatgaa gcgtttatgt
    51421 ccaaatgctt ggctgatcaa tttcgcgaac ccaagtggga tggtgacaga agcagttatc
    51481 agatatggca aatgggataa agtgattgga ttatgcaatg tgccagtgat ggcgatgatg
    51541 acagaaccag caatgcttgg caagaaaaaa gaagagctga tctacaagtt tgccggtcta
    51601 aatcatttcc attggcacaa agtagcagat aataaaggaa atgaccgcac ggcagaattg
    51661 attgatcgtt tatacgatga agagaatgga ttaccgaaaa atatctttga tatcccgttt
    51721 ttcaaagaac aattaaaaca aatgaacatg atcccttgtg gctaccaccg ctattactat
    51781 cgtgaagaag aaatgctgga acatgcttta gaagaatatc aaacgattgg tacaagagcg
    51841 cagcaagtga aagaaacaga agcagagcta tttgaattat acaaagatcc agaattagac
    51901 cataaacctg aacaattgca aaaacgaggt ggcgcttatt attcagacgc tgcctgtgaa
    51961 acgattgctt caatctatgg aaataaagaa acacagatcg tggtatctac aaagaatgat
    52021 ggagctgttc ctgacctgcc ggcagattgt gtagtagaag tcactgccta tgttggcgca
    52081 caaggagcga gaaatgtagc gtttggttct cttcctgctg ctgaaagagg ctggttgcaa
    52141 gtaatgaaga atatggaatt gctgacgatc gaagcagctg tgactggtga ttatgggaca
    52201 gctttacagg cgtttacgat caatccactt atacgatcag gaaagacagc aaaacggatc
    52261 atggatgagt tgtttattgc acaccagcag tatttaccgc aattcaagga gacaatcagc
    52321 cgattaaaag aagaaggaat cgaagttcaa gatgaactgg caagagaatt atcaggtaat
    52381 acacatataa tggaggaagt ataatgggat tcagaaaaga ttttttatgg ggcggtgcta
    52441 cagcagccaa tcaatgtgag ggcggttatg atgaaggtgg acgcggacta gcaaacgtag
    52501 atgttgtgcc aatcggaaaa gaccgttttc ctgttatcac cggaaagaaa aaaatgtttg
    52561 aatttgatga ggatcatttt tatccagcaa aaattgcaat cgatatgtat catcgttata
    52621 aagaagatat tgctttgttt gcagaaatgg ggttcaagac ttatcgaatg tccatcgcct
    52681 ggagtcgtat ttttccaatg ggggatgaga aagagccgaa tgaagctggc ttgaaatttt
    52741 atgaggatgc ctttaaagaa tgccaaaagc acggaattga gccacttgtg acgattacgc
    52801 attttgattg tccaatgcat ttgatcaaag aatatggtgc gtggcgttcc agagaactcg
    52861 ttggttttta tgaaaatctg tgtcgagtca tcttcaatcg ctataaagga cttgtcaaat
    52921 attggctgac attcaatgag atcaatatga tccttcatgc accattcatg ggcgcagggc
    52981 tgtattttga aaaaggagaa aatgaagaac aaatcaaata tcaagcggca catcatgagt
    53041 tggttgcaag tgccatcgca acgaagattg ctcatgaagt agatccggag aacaaagtag
    53101 gctgtatgct ggcagcagga gcatattatc catacacttg caatcctgaa gatgtttggg
    53161 cttctagaaa atcagatcga gaaaattatt tcttcattga tgtccagtca cgaggagaat
    53221 atcccgctta tgcattgaaa gaaatggagc gaaatggcat tcagatcgaa atggaaccag
    53281 gggatgccgc attgctgaaa gaacatactg ttgattttat ctctttttct tattatgctt
    53341 cccgagtttc gactaccgac aacgaactct tggaacagac tgcaggaaat atctttgctt
    53401 ctgtcaaaaa tccatatctg caagcaagtg aatgggggtg gcaaattgat ccattaggct
    53461 tgagaataac gatgaatgat atttatgatc gttatcaaaa accattgttt attgtagaaa
    53521 acggcttagg tgcagtcgat acgccagatg aaaatggtta tgtggaagat gattaccgaa
    53581 tcgattacct ggcagcgcat atccaggcga tgaaggatgc agtagaacta gacggagtcg
    53641 atctattagg ctatacgact tggggatgta ttgatttagt atcagctggt accggtgaga
    53701 tgaagaaaag gtacggattc atttatgtcg atcgtgataa ccaaggaaat ggcacattag
    53761 cccgttcaaa gaaaaaatca ttcaactggt ataaaaaagt aattgattca aatggagaaa
    53821 atctttcgaa cgattgatca atgatttata aaagtctatt gtttttcagc ggatttttcg
    53881 agtataataa agctattcga gaaacgcaag cagaacaata gacttttttt cgcattggct
    53941 gtaaagtatg cttacttgat taagtaaagg agaatctaca tatgtatgat gtaatcgtta
    54001 ttggcgcagg tcctgctggg atgactgctg ctttatatgc ttcacgttca aacttatccg
    54061 tattgatgat cgaacaaggt gcccctggcg gccaaatgaa caatacggca gaaatcgaaa
    54121 attatcctgg attcgattcg atcatgggcc ccgatctagc ttataagatg tatgaaagtg
    54181 tctcaagatt tgggacagaa aatgcttatg gaatcgttca agatatcaag gatcacggca
    54241 gccataaaga agtcatctgt gaagataaaa gttacgaagg aaaaacagtc attatcgcaa
    54301 caggatgtat ccaccgtaaa ttaggcgtac ctggcgagga agaatttgct ggacgaggcg
    54361 tttcttactg tgcagtctgt gatggtgcgt ttttccgtaa caaacgtcta gtagtcatcg
    54421 ggggtgggga ctcagcagta gaagaagcca tctatttgac acaatttgct tctgaagtag
    54481 tgatcgtcca ccgtagagat gaattacgtg cacaaaagat catccaagac cgtgcatttg
    54541 caaatgaaaa aatttccttt atgtgggata cagtcgttga cgaaatcgtt ggaaacgaaa
    54601 tggtagtgac aggtgtgaaa gcacgtaatg tcaaaaccgg caatgaattt gatttagcag
    54661 cagacggagt attcatctat gttggactgg atccattaac tgcacctttt agagaagcag
    54721 gtttgacgaa cgaagctggc tggattccaa cagacaacga aatgaaaacg aatatgccag
    54781 gcgtttttgc catcggagat gttcgtgaaa aagatttgcg acaaatcact acagctgttg
    54841 gtgacggcgg tattgctgga caacaagtgt ataaatactt ggagtcttta gaagaaacaa
    54901 cagcataagt ttaaaaagag tagaagaacg ggttcaattg ttcttttact cttttttttc
    54961 gcgtgaaatt tactgaatct gatttatgcg aacacgtcca acaccttttg ataaaagaat
    55021 cctttcttga tttaagcgtt ttttggtcct catactttca tatctaataa gtgaatgcta
    55081 tacttattaa gaaccatatt tcagaaagag gtgttatttt catgtcttgg gaacaagtgt
    55141 atgaacaatg ggtacatgaa gaaaatttag aagaaaactt aaaaaaacaa ttaaatgatc
    55201 tgggtgaaga tcctgaaaaa cttgaagatg ctttttatgc accgttggaa ttcggtactg
    55261 caggtatgcg gggaatctta ggacctggga tcaatcgaat gaacatctat acggtaagac
    55321 aagcaacaga aggactagca cgtttcatgg atacacaagg tgaagaaaca aaacgccgag
    55381 gtgtcgctat cgcttatgac tcacgccatc agtcaccaga atttgcaatg gaagcagcca
    55441 aaacactagc acatcacaat atcccgtctt atgtattcga aagtctgcgt ccaacaccag
    55501 agctttcttt tgcagttcgt catttgaaga cgtttgcggg aatcatggta accgcatctc
    55561 acaacccagc ggcttacaat ggatataaag tctacggaga agatggcgga caaatgcctc
    55621 ctgcagacgc agatgcattg acgaaatatg tccgtgaagt cagcaatccg ctaaaagtcg
    55681 aagtattatc tgacgaagaa gctaaacata gcgggttgat cacgatcatc ggagaagaag
    55741 tggacgctgc ttatctagaa gaaatcaaag cagtgacgat tgatcgtgaa ttggttgaaa
    55801 caatggggaa agatttgaaa cttgtgtaca cgccacttca tggaacaggg aaaatgttgg
    55861 gcgaacgtgc gttgaaacaa gctggattcg aacaattcgt actagttccc gaacaagctg
    55921 tggcagatcc agatttcact acggtcaaat cgccaaatcc agaagaacat tcagcttttg
    55981 aatatgctat acgacttgga gaaaaagaag gagctgactt gctgattgca acagatcctg
    56041 atgcagaccg tttaggcgca gcggttcgtt tgccagatgg ttcttatgaa gtattgactg
    56101 ggaaccaaat cggagcattg tttgctcaat atattttgga agcacatcaa tcaagaggca
    56161 ctttaccaga aaacgcagcg gtattgaaat caatcgtttc cagcgaattg cctacagcaa
    56221 ttgcggaaca ctacggtgct accatgttca atgtgttgac agggtttaaa ttcattgctg
    56281 aaaaaatcca acaatttgaa gaagaccatt cacatacttt tatgtttgga ttcgaagaaa
    56341 gttatggtta tttagttaaa ccttttgttc gcgataaaga cgcgatccaa gcattgcttt
    56401 tattagcaga agtagcagct tactataaaa aacaaggcaa aacgttgtat gacggcttgc
    56461 aaacgatttt tgaaacctat ggctatttcg cagaaaaaac gatttctgtg acaatgagcg
    56521 gacaagaagg ttctgcgaaa atctctgctt tgatggaaaa attccgtaaa gaagcaccga
    56581 cagaatttgc cggagcaaaa gttattcaaa cagaagactt taaagaattg acgagaacag
    56641 accgtgaagg aaacgtagaa aaactaacaa caccgccatc cgacgtattg aagtatacat
    56701 tggaagaaaa tggctggatc gctatccgcc cttcaggaac tgagccgaaa atcaagtttt
    56761 atattggtgt aaaaggaagc tctacagaag acgcagccga aaaagtagcc tcttatgaag
    56821 cgattatcaa aaagatgaca gaagaataaa aaacttgaga ctgatggaaa aagtggtaaa
    56881 ctttagaagt cagtcttttt tcaagttgga ggtgaatgga ttgccagaca cgcaaaaatc
    56941 aaaagtagag aaagtcagtc aattattcaa aatgttgagt gatcccactc gtcttaaaat
    57001 tctgctatat ctgaaagacg gggaacaaaa tgtgacagca atcacccaag cagtggaaat
    57061 ggagcagtct gctgtatcgc atcaactacg tttattaaga gaaaatcatg tcgtcaagtc
    57121 tcatcgcgag ggaaaagcga ttttgtatag tcttgatgat tctcatgtac tggatatttt
    57181 gaaccagaca ctcaaacatg tggaacatca ataacgatag gtgaaaatag ataaacactg
    57241 gagtgtgaca acgaagaaat ttcgttgtca cactccagtg ttttttaaga aaaagacaaa
    57301 aatgattaat cgcttttttc tgttgtttca gttccataat aactatgatc ttctaggtct
    57361 aaagcattca aaataagcga agccgtttct tcaatagata aagatgccac attgatcact
    57421 acacatccta gtttttcata aagatcattc gcaaaatcga gttcagctct aattttttcg
    57481 atatcggaat aagcagtatc agggtttaat ccgtaagagc gcatccgttc tttacggata
    57541 ttatttaaaa tatctgggtt attcgttagt cccacgattt ttttaggatc gatttcccac
    57601 agctgtttgg gaatatgtgc ttggggaatc aaaggtaaat tagcaacttt taaattttta
    57661 tttgctaaaa acaagctcaa aggcgttttg gaagttcggg atacgcctag caaaacgaca
    57721 tctgcttcta aaaaaccacg aggatctttc ccatcatcgt attttacagc aaattccatt
    57781 gctttgatcc gtttgaaata gttctcattc aataaatgga gggcaccagg ttgccgaatt
    57841 ggcttgatac cagttaaacg ttcaatttct gatatcggtg gcgtcatcac atcgaaacta
    57901 aataattgat gttcttcaca aaaagaacgt gttatctcga tcagttcttg attgattaat
    57961 gtatgcagga caagtgcttt ttcttctaat gcatcttgca atgcctgcct taatgttttt
    58021 tcatctgtca cgaacgttct gcggaatagg ttgaactcaa cggtaggata ctgagccata
    58081 gtagcttgcg ctagttttgt cgcagtttct ccagcagagt cggagatgac aaaaaaagta
    58141 acgatttctg tagaatcgga agacatggat aaaacctcac ttgcattttt tttatgttta
    58201 ttataccata ctctagaatg cgtacttcgt aatcattttg atttggagga ataagtcatg
    58261 aaaaaacaga cattgatcga acaatcatta gaaacattga aaaataacgg ttttaaatat
    58321 actaaaaaaa gagaagcatt gttaacttat ttagtgaagc gtaaccgcta cgtatctgct
    58381 aaagaagttt ttgactatat gaatcaggaa ttcaaagggg taagttatga tacgatttac
    58441 cggaatctcc atgattttgc agagatcgat ttactggaag aaaccgattt aaatggtgaa
    58501 atgaagtttc gttttcattg ctgtcagggg gaaatcggcc atcaccatca ttttatctgt
    58561 acagtgtgcg gaacaacgaa agaaatccat atgtgtccaa tgaattattt cgaagaacag
    58621 ctaggtggat gcacgatcga agggcataga ttcgagattt tcggaagatg tgaaaattgt
    58681 caataaggat ttaaataaaa taacatttga taatttaaaa aaaaagcaag atttccttga
    58741 cggtcaaaac agcattagat ataatattta caggacagtc ttgttttttg agatatattt
    58801 caataacaat tacggttact ttttaagctc ggagggaggg aattaacatg tcaaaaacag
    58861 tcgttcgcaa aaacgaatca cttgacgatg ctcttcgtcg cttcaaacgt tccgtgtcaa
    58921 aagcgggtac tttacaagaa tctcgtaaac gtgaattcta cgaaaaacca agtgtaagac
    58981 gcaagaaaaa atctgaagca gctagaaaac gcaaaaaatt ctagttctgt gatgaattgg
    59041 agttggtagt gtgtcactac taagtagatt aaacgatgac atgaaagcag cgatgaaagc
    59101 gaaagataag gaatctttac aagtcatccg aatgatcaag tcatctatac aaaacgagca
    59161 gattaaagaa ggtcacgact tgacagaaga agaagaacta acggtgttgt ctcgcgaaat
    59221 gaaacagcga cgtgattctt tgcatgagtt tgaagaagca ggacgtgacg atttagctga
    59281 aaaagtaaaa agtgaaattg taatagtcga aaaatatatg ccggaacaac tttccgacga
    59341 agaaattcga caacttgttc aagaagcaat cactcaaact ggtgcttcat caatgaaaga
    59401 atttggcaaa gttatgggtg caattatgcc taaagtcaaa ggaaaagcag atggaaacca
    59461 agtgaatgcg attgtgaaag aactattaca atcttaattt tatcatttaa actgcggaca
    59521 gtgattgtcc gcagtttttt gtgttcgtaa aaaaagaact aaaaaacagc tagaagaaag
    59581 aataagattc tttcttctag ctgttttttt cctgtttaat atctcttatg ttaaattgtg
    59641 tttgatttgg ttattcttct ggtagttcat ttgcttcttg tttataccct gataggcgat
    59701 aggcgtaata aaagagtaag ctgccagcgt aaccaagtaa cgttgggacc atccagccaa
    59761 gtcctaatgc agcaaaagga acgtgatgat gataaaatcc tagtaagctt gctacgacag
    59821 tttgctgact cactaatgca ggcgaatttg ccaacatatc caatacggct ggaacggcag
    59881 aaaagaacat cgttacttga tagacaaatg gactcttttg gaagaatttt gctgttaagg
    59941 aaagcaagat taacgcaagt gacaatggat acaaaagcat caatactggt aaagaccatt
    60001 ggatgatatt atccaatccg gcattcgcaa cgacaaaaga gacaaaagtc gttaaccgca
    60061 gccatgtcat ataactgact ttagggaaca gtttatggaa atcttgtgca aaagaaacaa
    60121 cgagtcccat agcggtagtg aagacaccta atgtgaccat tacccctaag aagctagcac
    60181 cgaagttacc taaataaaaa ttagtgatct gagaaagtgc agttccgcca ttttctgata
    60241 aaggcatctt attcaaactc atcgcaccga gaaggaccag accaaaataa atcatgactt
    60301 ctaacgcaac gctcaggcta ccagcttttg ctgttaattc tgtgactttt ttatctttaa
    60361 aaccaagatc acgaactgca tggacaaaag tgacacttaa agcaagtaaa gctactgcat
    60421 caaccgtgtt gtatccttct aaaacgccat ttgtcaaagc gttcgtctga taagaatctg
    60481 cagcagattg gtttagattc cccatgggtt ggaataaaga aacgacaaaa atgatagcta
    60541 ataatgcaag aaacaaggag tttaaatact ttccgacata tttgaccaaa cgtgttggat
    60601 gtacagtaag gaaataagca agtaaaaaga aaagagcaga gaataaaaac atacctaatg
    60661 aatcatattt ttcaggcaaa aagggtttag cagccatttc aaaagcagtt gctgctgtcc
    60721 gcggtgtacc aaagaatggt ccgatcgtta aatgaacaag gatcaagaac aaggcagcat
    60781 accattttcc gacaggttta gctaagtcat acagaccatc actttttgtc actactacag
    60841 ctaagatagc taataatggg aaaagagcgc ctgagatggc aaatcctaca ccagcgctga
    60901 tccagtttga tccagccatt tgacctaagt ggacaggaaa gatcagatta cctgacccga
    60961 agaacatccc gaagataagt gaactgacca tcaataattg cttccatgaa taagtttttg
    61021 tttcttccat aaatttttcc tccaataatt atcattttga gtaacaaaat aaacgttgca
    61081 gaaactagaa atgccactaa tatcagattc catgaaaacc accaccttaa aatataaaaa
    61141 aaagcccttt tccacttggg aaaagggctc tagattctag aacggtacca ccttttttcg
    61201 cttaaaaaag cttcttcatt cacggacgct tttcgataaa gttatccgct agcctgataa
    61261 tggaggcagc cacaatgatc tacgttttcg acattgactc ggaaatgatg ttcgtctatt
    61321 cgatttattg tcatttctca cttacatgac tcactgggaa taaatcgctg aatagcctac
    61381 tgttttcctc gacgctttgc tgtatattta tttgttgacg ttataataca cctgtttttt
    61441 ttgaaatgca agaatatttc aaatattttg ttttcttttt ttgaaacaag aaaaataccc
    61501 agagaatact tgctaagtta gacggaatac ttgtcctcta tcttatgcat agcttacatg
    61561 atcagctttt tttattcaaa cgagaccttt agagaatgga agcagctatt ttttaacgtt
    61621 tcttggtact tatggttaag caactctttt agaacctttt cttatttgct atatagtttc
    61681 ttctgagtat ggtaaaatag gaaagaatga atcgaaaaaa aggggatgtc tatttttgac
    61741 ggaagagtct agttctttag agattagatt gacggctgct gatgatgtca gcatgctttt
    61801 aggtgcacac gacaagcata taaaactgat tgaagaaaca acagaaacaa ccatccatac
    61861 acgtggagaa atcattcaga tcattggcga acaatcccag atttccttgg ctgcctcagt
    61921 cattcggaca ttgcaggaat tgatcaaacg aggaatccat gtaagtacac cagatgtcgt
    61981 gacggcactg aaaatggggc aaaaaggcac attagattat tttgttgaaa tgtatgagga
    62041 agagatcgtc aaagaccgta atggaaaacc aatacgggta aaaaacagcg gccaaaagaa
    62101 atatgtcgaa tcggtagcca aacatgatat cgtatttggc gtaggaccag ccgggacagg
    62161 aaaaactttt cttgcagtag tattagcaat cgctgccttg aaaaaaggtg aagtgcaaaa
    62221 aatcattttg acgcggcctg ctgtggaagc aggggaaaat ctaggctttc ttcccgggga
    62281 tctaaaagaa aaagtagatc cttatctgcg tcctgtttat gatgctttat accaaatttt
    62341 tggattagat catacgaacc gattgatgga acgaggcgtg atcgaaattg ctcctttagc
    62401 ttacatgcgg gggcggacat tggatgatgc attcgttatt ttggatgaag cacagaacac
    62461 gacagttgct cagatgaaaa tgttcctgac acgtcttggt tatcaatcaa aaatgattgt
    62521 caatggagat acgagtcaga ttgatctgcc tcgagggaca acaagtggtc ttgttcatgc
    62581 agaacgaaca ttaaagcaaa tcaagaaaat cgattttgtt aattttgaag caagtgacgt
    62641 tgttcggcac cctgtagttg ccgaaatcat caaagcttat gaaaaggcag atctccatca
    62701 agaataagct ttgcttagga ggataagcac atgttgaata aaacactgca gaaactacaa
    62761 gaaaaacttg gaaaggcagc attgcctttc ctgctgttct tgttttttat cattatctgc
    62821 ggcgttacat ttagtagtgt caaacaaaag gccaatgatt tcagagaagg ccaagtagca
    62881 gaagaaagta tccgtgcgaa caaaacgatc gagaacacgg aagaaaccga acaaaaaaga
    62941 aaactggcag cagaagcagt cactccagaa tacacttacc aaaaagatct tgcagatgac
    63001 cagaacaatc ggatcaagca actatttgaa ttgatcgata aaacgaatga tgccatcaac
    63061 aaatcttatg acgaaaagat tgataaggca aaggaagatg aaaatatccc aaagcctact
    63121 acagaagagc ggatcgccgc gttgaagaaa aattttgaaa atgtgaatgc agaaaatgtt
    63181 gcgttttatc aaaaactacc gaacaatttt tattcgacga tatttgaaat gactgaaact
    63241 gagatccata ctgtgaggga tgagagtctg aagctcattg acgaacagat gagcaaacaa
    63301 gttcgtgaaa gtgaattgga agcttttaaa caagaagcag aagagcaaat ccaatatttg
    63361 aatgtcacac cagcacaaca acaaatgatt cgctacttag tggatcaagg tatcgtagtg
    63421 aatgacgtat tgaatgagaa aaagacagaa gaattgaaac aatccgcacg agaagctgta
    63481 caaccagtga tgatcttcca aggagaaatc attgttcgtg aaggtaatca aatcgatgct
    63541 tcagctatga aaaaattgga attactagga ctgactaatc aaacaacttc gatttttccg
    63601 ctaattgcca tggttttagc tgttttgctt caaatcggcg tactgattta taattcactt
    63661 caattccaaa atgcaggaga aagaacaaaa tacgtgcttt tctatgtgac agctatgtca
    63721 gtcagcatag tgctgatgaa atttttccaa ttattccaaa cggaacaagc tacttatatc
    63781 ccgctgtttt atcccgctgc ttttgcacca cttgttttaa gctttttctt gaaccgaaga
    63841 gcagggatca tggctggtat tttccaagca gtatctgcac ttttcatttt ttatgattcg
    63901 attggcacga actttctaac gattatcttg atgtcttatg ttttctctgg gctcatggca
    63961 acgattgtca gacgcaaacg gatctcagaa caaggattat ctgctgccat gtgggtcatt
    64021 atttttcctg tgatgatgga tgtcattttg atcgtctatc aaggaatgag ctttggtgat
    64081 gcaacgactt ggttaatgct tgtctgcggg ttcactggtg cactcttctc attcttgctg
    64141 acgatcggtc tacacccata tattgaattg atggtcaatg atgacagtgt aattgtatta
    64201 aatgaactca gcaatccaaa tcatccttta ttgaaacagt tgctggaaga agcgccaggt
    64261 acttatcatc atagtatgat ggtagctaat ctaagtgcta acgctgtagc agaaatcggc
    64321 ggacgttctt tgctaactcg agtagcttgc tattatcatg atatcggcaa gatcaaacat
    64381 gctagctttt tcgttgagaa cttgccttca ggagcggaaa acccgcacaa tttcttattg
    64441 ccagaagaca gtaaacatat tatctttgga catgtgacag atggagccaa aatattggaa
    64501 gattataata tgccgcaaat ggtgatcgat atttgcaaac agcatcatgg aaccacgcta
    64561 atgcgttatt tctacatcaa agcgaaagaa cgcaatccgg aagtaacaga agagcaattc
    64621 cgttatcctg gaccaaaacc gcaaagtcgt gaagcaggga tcgtcaatat tgctgacagt
    64681 tgtgaggctg cagtacgggc aatggatcat ccgacgagcg aaaaaatcga aagttttgtc
    64741 cataatctta tcgaagagcg tatttccgat ggtcaattag acgatagtgg actgaccttg
    64801 aaagaaatcc gaaaagtgga aaaatcattg atcagcggtt taagtagcac cttccattct
    64861 cggatcaaat atcctaaaat gaaaacagaa gttgagaaga taaaggaaga acaagaagaa
    64921 tagaaagaag cgatgtataa tggatattac ctttatggat gaaacaaacc aagtctcaga
    64981 agagaaaatg aaagaaatcg atgacttgct gcaatttgca gcatcttatt taaagctgcc
    65041 agaggaaaca gaaatgtctg ttactttcat ggacaatgcg gcaatccaag taatcaatcg
    65101 tgattaccgt ggaaaagatg caccgacaga cgtcatcagt tttgctctgg aagaagaagg
    65161 cgaagacgaa atcccagtga tttttgaaga tggagagaat ccattgcctc gtgtactagg
    65221 agacatcatg atttctattg aacgtgctgc tgaacaagca gaagcttacg gccattcata
    65281 tgatcgggaa cttggctttt tagcggtaca tggattcttg catatcaacg ggtatgacca
    65341 tatgacacca gaagatgaga aagaaatgtt tggtttacag aaagagatct tggatgccta
    65401 tggactcaaa agataaaaac gtagagaaaa acaaacattt cctgatgtct gtggaatttg
    65461 ctgtccaagg agtcaaaacc gtttttgagg aagaacgcaa tatgaggaaa catgttgcgt
    65521 tcggtatcct tgctttattt atgggcgtga ttttccagct tgatcggata gagtggttat
    65581 ggctgctttt agctgttttt ctcgtttgga tagtagagat catcaatact gtttttgaaa
    65641 atgtagtgga catgtttacg gattttcatt ttcatccgat tgggaaaaaa atcaaagata
    65701 tggcagctgg agcagtttta gtgaccgctt gttttgctgt catcattggg ttgattttat
    65761 ttgtccctaa aatatatcat ttgttgttta attaaaaagg agaacgtata tgccagaaca
    65821 taaatcaggg tttgtcgcaa tcgtcggccg cccgaatgta ggaaaatcca cattattgaa
    65881 ccgaatcgtg ggtcaaaaaa ttgccattat gagtgacaag gcacagacaa ctagaaataa
    65941 aatccaagga gtatatacta caccagaagc tcagctgatt tttatcgata caccaggaat
    66001 ccataaacca aagcatcgtc taggagattt tatggtagaa gcagcttaca gtgcgttgaa
    66061 agaagtggat gcagtcttat ttatgatcag tgctgatcaa aaacgcggcc ggggagacga
    66121 ttttattatt gaacgtctaa aaaacgtgca ttctccagtt tatctgatta tcaataaaat
    66181 cgataagatc catccagacg agttgctggg aatcatcgaa gattattcga cgcagatgcc
    66241 ttttgcacaa gtgattccga tctctgctac agaaggaaac aatgtcgaac gcttaatgga
    66301 tgtattagtc agtgaaatgc cagagggccc acaatatttt ccagatgatc aagtgactga
    66361 tcatcctgaa tactttatcg tttctgaatt gatccgtgag aaagtcttgt ttttaacacg
    66421 agacgaagtg cctcattctg tcgcagtcgt tgttgattcg atgaagcgaa acgagaatga
    66481 taaaatccat atccaggcca cgatcatcgt cgaaagagat agccaaaaag gaatcattat
    66541 cggcaaaggc ggaaaaatgc tgaagcaagt aggaacgaaa gcaagaaagg atatcgaaaa
    66601 tcttttagga gacaaagtat ttctagaact ttgggtcaaa gtccaaaaag actggcgaga
    66661 caaaaaagtc tatctgcaag actttggcta ccgaaaagac gaatactgat attctgaact
    66721 tcttataggt taagtataga aataaactgg ataaaaatcc gcacgttgag aaaaacggcg
    66781 gaagatcgaa acataaataa tctggatcgg cagaatctgc ttttccagat ttatttttta
    66841 ggagggagaa tatgagccag cttgcagaat cgaaaggctt gattttatct agccgtaatt
    66901 acaaagaaaa agataaatta gtcaaaatct ttacggaatc ttctggcaaa atgatgtttt
    66961 atatcaaagg aatccatcgg caaaatcagc cattagctcc ggctttgctc ccctttaccg
    67021 aagcagtcta tatcggtaat tttcgaagtg aaggtttgtc gtttttaagc agtgtcaaag
    67081 acgtccatgc gtttcggaac atccaagaag atatcttttt gagcggttat ggtacctatt
    67141 tgctaaatct agtagatgca gcaatcgaag atcatcaata tgacccgcat ttattccaat
    67201 ttacgcaaca agctctgcag cggatggatc aaggagatga tgcagaaatc atcacgaata
    67261 tctttgaagt tcaaatactg caacgcttcg gaatcgcgcc aatttggacg cactgtgtcg
    67321 tatgcgggga aacaaaagga aaatttgact attcttccaa atctggcggt gtgatatgcg
    67381 agaaacactg gccaatggac gaacaccgtt atcatgcaga tcctcgcgct gtttatttcg
    67441 ttcgaatgtt ttcggctatt tcttatgata agatcagtgg aatccacttg aaagaagaaa
    67501 cgaagcaggc aatccgtgac ctcattgacc agttgtatga tgaatatgtc ggtattcatc
    67561 taaaaagcaa aaagttcatt gatcaaatga agtcttgggc acacgtatta aagacgccag
    67621 agaaaaatat gcctgaaaaa gagatacctg aaaaagaaga ataaatgaaa gatacaacaa
    67681 aaaaatcaaa actatttgac aaatacaggg acttccgtct attattaacc gtagattaaa
    67741 tagatcgttc actgagagga tctattatat aaacaagaaa actgcaaaat gaatacgtac
    67801 attgaccaag atcagtaaaa aacacgtggt ttagcgagtt cgggagagtg gaagccggac
    67861 ccaaacgatt tttgaagagg cacttgcgag tacgataaat gaagctgccg aatctttatt
    67921 cggaagtagg gtggaaccgc gaaattattc gtccctatgt ctgtaatcag acatagggac
    67981 tttttgtcgt tttctttcct gtattcttat tgacagcaac gcttaaaaat tgaaaggggc
    68041 aaaacagatg agtaaaaaat tgaccgtcca agatatgatt ttaacattac aaaaattttg
    68101 gtcatcaaac ggctgcatgc tgatgcaagc ttatgacaca gaaaaaggag caggtacgat
    68161 gagcccatat acattcttac gggcaatcgg accggaacca tggaatgctg cttatgtaga
    68221 gccatcccgc cgaccagctg acgggcgtta tggagaaaac ccaaaccgtt tgtatcagca
    68281 tcatcaattt caagtcgtaa tgaaaccttc acctgaaaat atccaagagc tttatttaga
    68341 aagcttgcgt cttttaggaa ttgatccatt agaacacgat attcgttttg ttgaagacaa
    68401 ctgggagaac ccatcgatgg ggtgtgccgg tgtgggttgg gaagtatggc ttgatgggat
    68461 ggagatcaca caattcacgt acttccagca agtcggagga ctaccttgta aaccagttac
    68521 ttctgagatc acttatggac tagaacgtct tgcttcatat attcaagaag ttgaaagtgt
    68581 ctatgatctg gaatgggctg atggtgtgaa atatggcgag atcttcaaac aaccagaata
    68641 tgaacattcc aaatattctt ttgaagtaag tgaccaagac ttgctactaa gcaactttga
    68701 ccgttttgaa aaagaagcta aacgctgtat cgatgaaaat cttgttcatc cagcgtatga
    68761 ttatattttg aaatgcagcc atacattcaa tttattagat gctcgcggtg cagtatccgt
    68821 aacagaacga gcaggttatc tttcccgtat ccgcaacatg gctcgtgctg tagccaaaat
    68881 ctttgtggca gaacgcgaaa aattaggatt ccctttattg aataaaaaca cagcaaatcc
    68941 agaaaacatg aaggaggctg aataagatgg caaaagactt attacttgaa atcgggctgg
    69001 aagaaatgcc tgctcatgtt gtcacaccaa gcatgaaaca attagaagaa aaaacagcaa
    69061 aatttttaga tgaaaaccaa ttgacttatg atacgatcga aacgttttct acaccgcgcc
    69121 gcttagcaat caaagtaaca aacattccag aacgtcaagc agacagtgaa gaagaagtca
    69181 aaggaccagc caaaaaaatt gctttggacg cagatggaaa ctggacgaaa gcagctgaag
    69241 gatttgtccg cggacaaggt ctgacgacag atgctatcac ttttagagaa ttaaaaggcg
    69301 tggaatatgt ctatgtaacg aaatacactc acggcaaaga agcgaaagaa gtattgacag
    69361 gtttagcaga tgtcattaaa agcctcactt tcccagtaac gatgcattgg gcagaatacg
    69421 attttgaata tatccgccct attcactgga tcgtggcatt gttagatgac gaagtaatcc
    69481 cgtttgagat cttagatgtc aaaacaggca gagcctctcg aggacatcgt ttcttaggcg
    69541 aagatgtgac atttgcgcat gcaaaagaat atgaagaaaa attgaatgaa caattcgtta
    69601 ttgcttcacc gcaaaaaaga caagaaatga tcgttacaca agcaaatgaa ttagccgaaa
    69661 aaaatcactg ggttattgat ctagacgaag atttactaga agaagtcaac aatttagtcg
    69721 aatatccaac tgtcttttct ggcgacttcg aagaaaaata tctttctgtt cctgaagaag
    69781 tactagtcac ttctatgaaa gaacatcaac gttatttcga ggtacggaac gatcaaggta
    69841 tgctgcttcc tcattttatt tctgctcgta atggtgatcg ggtaaaattg gaaaatgtgg
    69901 ttcgaggcaa tcaaaaagta ttgacggctc gtttagaaga tgcggaattc ttttataatg
    69961 aagacaagaa agcttcaatc gaccaatacg ttgaaaaact aaaaactgtt actttccatg
    70021 aaaaaatcgg tagtatctat gagaaaatgc agcgggtaca agtcattgcc cgtatcattg
    70081 gcgaacaagt aggactatct gaggaagaat tgatggatct gcaacgagct gctgaaatct
    70141 ataaatttga tctagtaacc aacatggtag gggaatttcc tgaactacaa gggatcatgg
    70201 gcgaaaaata tgctctgctg aaaggcgaga aacctgctgt agcaacagcg attcgtgagc
    70261 attacatgcc tatatccagt gaaggagagc tacctcagtc tacagtaggt tctgttttag
    70321 cgattgcaga taaattagat agcatttttt ctttctttgc agttgggatg attccaagcg
    70381 ggtcaaacga tccttatgcc ttacgtcgcc aagcatacgg tgtcgttcgg atcatcgaat
    70441 cgaaaaaatg ggcctttcct ttgtctgttc tgcaagaaac gattagtgat gttatcagta
    70501 aagatacgga tcgtttcggc attggtttat cagaagggca acaacaggtg atcgacttta
    70561 tcaaaggccg tctgcgccaa ttattgacaa cgaagaatat tcgtcatgat gtcatcgaag
    70621 ctgttttgaa tgcagaacaa aaagatctga caaaagtatt tgcagcagca caattgttca
    70681 agcagcatct tgcagatgaa gacttcaaac catcgatgga agctttgaca cgtgtcgtga
    70741 acttagcgaa aaaagcggag cttgaaaaac aatcagaagt agaccctgaa ctgttcgaaa
    70801 atgaagcgga gaaagaactg cacaaagcag tagaggcaat gaaaaaaagc tttgctgacc
    70861 aaacgatcaa cgaaaattat gatcagcttg taagccttcg cccactgatc gaaaattatt
    70921 ttgatcagac gatggtcatg gcagaagaac cagcagtacg taagaaccga ttaacgcaat
    70981 taagtcaaat tgctgccatg gctttgtcgc tagcaagttt agatcaaatc atcacaaaat
    71041 aatttctaat atgtaaataa accagagaat aggcacaaaa
//
LOCUS       DACDXE010000005        64262 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.005, whole genome shotgun sequence.
ACCESSION   DACDXE010000005 DACDXE010000000
VERSION     DACDXE010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 64262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64262
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.005"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            567..1404
                     /locus_tag="IWM23_000308"
                     /pseudo
     CDS             567..1404
                     /locus_tag="IWM23_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            1469..1903
                     /locus_tag="IWM23_000309"
     CDS             1469..1903
                     /locus_tag="IWM23_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9882984.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(1971..3293)
                     /locus_tag="IWM23_000310"
     CDS             complement(1971..3293)
                     /locus_tag="IWM23_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAP9882985.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(3447..5087)
                     /locus_tag="IWM23_000311"
     CDS             complement(3447..5087)
                     /locus_tag="IWM23_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAP9882986.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            5417..5887
                     /locus_tag="IWM23_000312"
     CDS             5417..5887
                     /locus_tag="IWM23_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAP9882987.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            6516..7619
                     /locus_tag="IWM23_000313"
     CDS             6516..7619
                     /locus_tag="IWM23_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9882988.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            7903..8583
                     /locus_tag="IWM23_000314"
     CDS             7903..8583
                     /locus_tag="IWM23_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9882989.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            8673..9707
                     /locus_tag="IWM23_000315"
     CDS             8673..9707
                     /locus_tag="IWM23_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9882990.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            9721..10056
                     /locus_tag="IWM23_000316"
     CDS             9721..10056
                     /locus_tag="IWM23_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882991.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            10040..12520
                     /locus_tag="IWM23_000317"
     CDS             10040..12520
                     /locus_tag="IWM23_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9882992.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            12576..14135
                     /locus_tag="IWM23_000318"
     CDS             12576..14135
                     /locus_tag="IWM23_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9882993.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDCPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            14132..15496
                     /locus_tag="IWM23_000319"
     CDS             14132..15496
                     /locus_tag="IWM23_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9882994.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            15667..16737
                     /locus_tag="IWM23_000320"
     CDS             15667..16737
                     /locus_tag="IWM23_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9882995.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            16752..17420
                     /locus_tag="IWM23_000321"
     CDS             16752..17420
                     /locus_tag="IWM23_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9882996.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            17440..18018
                     /locus_tag="IWM23_000322"
     CDS             17440..18018
                     /locus_tag="IWM23_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9882997.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            18421..19332
                     /locus_tag="IWM23_000323"
     CDS             18421..19332
                     /locus_tag="IWM23_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9882998.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(19588..20505)
                     /locus_tag="IWM23_000324"
     CDS             complement(19588..20505)
                     /locus_tag="IWM23_000324"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAP9882999.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            20707..21144
                     /gene="zapA"
                     /locus_tag="IWM23_000325"
     CDS             20707..21144
                     /gene="zapA"
                     /locus_tag="IWM23_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAP9883000.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            21202..21732
                     /locus_tag="IWM23_000326"
     CDS             21202..21732
                     /locus_tag="IWM23_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAP9883001.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            21864..24224
                     /locus_tag="IWM23_000327"
     CDS             21864..24224
                     /locus_tag="IWM23_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9883002.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            24311..24625
                     /gene="trxA"
                     /locus_tag="IWM23_000328"
     CDS             24311..24625
                     /gene="trxA"
                     /locus_tag="IWM23_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAP9883003.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            24807..26585
                     /gene="uvrC"
                     /locus_tag="IWM23_000329"
     CDS             24807..26585
                     /gene="uvrC"
                     /locus_tag="IWM23_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAP9883004.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            26730..27629
                     /locus_tag="IWM23_000330"
     CDS             26730..27629
                     /locus_tag="IWM23_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883005.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            27622..28884
                     /locus_tag="IWM23_000331"
     CDS             27622..28884
                     /locus_tag="IWM23_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883006.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(28978..30270)
                     /locus_tag="IWM23_000332"
     CDS             complement(28978..30270)
                     /locus_tag="IWM23_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9883007.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSECRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            30554..32452
                     /locus_tag="IWM23_000333"
     CDS             30554..32452
                     /locus_tag="IWM23_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAP9883008.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(32751..33470)
                     /locus_tag="IWM23_000334"
     CDS             complement(32751..33470)
                     /locus_tag="IWM23_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAP9883009.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            33807..34139
                     /locus_tag="IWM23_000335"
     CDS             33807..34139
                     /locus_tag="IWM23_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883010.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            34126..36129
                     /locus_tag="IWM23_000336"
     CDS             34126..36129
                     /locus_tag="IWM23_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAP9883011.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            36148..36618
                     /locus_tag="IWM23_000337"
     CDS             36148..36618
                     /locus_tag="IWM23_000337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAP9883012.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            36639..37223
                     /locus_tag="IWM23_000338"
     CDS             36639..37223
                     /locus_tag="IWM23_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAP9883013.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            37238..38224
                     /locus_tag="IWM23_000339"
     CDS             37238..38224
                     /locus_tag="IWM23_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAP9883014.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            38214..38525
                     /locus_tag="IWM23_000340"
     CDS             38214..38525
                     /locus_tag="IWM23_000340"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAP9883015.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            38547..40328
                     /locus_tag="IWM23_000341"
     CDS             38547..40328
                     /locus_tag="IWM23_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAP9883016.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            40321..41697
                     /locus_tag="IWM23_000342"
     CDS             40321..41697
                     /locus_tag="IWM23_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAP9883017.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            41704..42339
                     /locus_tag="IWM23_000343"
     CDS             41704..42339
                     /locus_tag="IWM23_000343"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAP9883018.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            42369..43724
                     /locus_tag="IWM23_000344"
     CDS             42369..43724
                     /locus_tag="IWM23_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAP9883019.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(43916..45310)
                     /locus_tag="IWM23_000345"
     CDS             complement(43916..45310)
                     /locus_tag="IWM23_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9883020.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            45402..46373
                     /locus_tag="IWM23_000346"
     CDS             45402..46373
                     /locus_tag="IWM23_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAP9883021.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            46370..47152
                     /locus_tag="IWM23_000347"
     CDS             46370..47152
                     /locus_tag="IWM23_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAP9883022.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(47321..47692)
                     /locus_tag="IWM23_000348"
     CDS             complement(47321..47692)
                     /locus_tag="IWM23_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9883023.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            47826..48269
                     /locus_tag="IWM23_000349"
     CDS             47826..48269
                     /locus_tag="IWM23_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9883024.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(48312..49121)
                     /gene="yidA"
                     /locus_tag="IWM23_000350"
     CDS             complement(48312..49121)
                     /gene="yidA"
                     /locus_tag="IWM23_000350"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAP9883025.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(49140..50507)
                     /locus_tag="IWM23_000351"
     CDS             complement(49140..50507)
                     /locus_tag="IWM23_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883026.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(50491..51321)
                     /locus_tag="IWM23_000352"
     CDS             complement(50491..51321)
                     /locus_tag="IWM23_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAP9883027.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            51484..51921
                     /locus_tag="IWM23_000353"
     CDS             51484..51921
                     /locus_tag="IWM23_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAP9883028.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            51997..52617
                     /locus_tag="IWM23_000354"
     CDS             51997..52617
                     /locus_tag="IWM23_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAP9883029.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            52806..53312
                     /locus_tag="IWM23_000355"
     CDS             52806..53312
                     /locus_tag="IWM23_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAP9883030.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            53329..54228
                     /locus_tag="IWM23_000356"
     CDS             53329..54228
                     /locus_tag="IWM23_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAP9883031.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            54315..55199
                     /locus_tag="IWM23_000357"
     CDS             54315..55199
                     /locus_tag="IWM23_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9883032.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(55391..55462)
                     /locus_tag="IWM23_000358"
     tRNA            complement(55391..55462)
                     /locus_tag="IWM23_000358"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(55427..55429),aa:Arg,seq:ccg)
     gene            55522..56856
                     /gene="rpoN"
                     /locus_tag="IWM23_000359"
     CDS             55522..56856
                     /gene="rpoN"
                     /locus_tag="IWM23_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAP9883033.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            57059..58096
                     /locus_tag="IWM23_000360"
     CDS             57059..58096
                     /locus_tag="IWM23_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAP9883034.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            58137..59138
                     /gene="gap"
                     /locus_tag="IWM23_000361"
     CDS             58137..59138
                     /gene="gap"
                     /locus_tag="IWM23_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9883035.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            59248..60438
                     /locus_tag="IWM23_000362"
     CDS             59248..60438
                     /locus_tag="IWM23_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAP9883036.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            60542..61297
                     /locus_tag="IWM23_000363"
     CDS             60542..61297
                     /locus_tag="IWM23_000363"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAP9883037.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            61413..62711
                     /gene="eno"
                     /locus_tag="IWM23_000364"
     CDS             61413..62711
                     /gene="eno"
                     /locus_tag="IWM23_000364"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAP9883038.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            63110..64249
                     /locus_tag="IWM23_000365"
     CDS             63110..64249
                     /locus_tag="IWM23_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAP9883039.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        21188 a        11273 c        13516 g        18285 t
ORIGIN      
        1 caaaagaatg aattaataag atataaaaaa agggattgtg acaaacgtct tgtcacaatc
       61 cctttttcca aataaacgat gttcaatcag ctgcccctcg gcattcgctc gaaaaacggc
      121 gaaatatgaa gaactatttt gccatttgtt tctctaataa tcaggtcaaa acgttgactt
      181 cacagtctct tccttaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt
      241 caacaaacat gagaagctgt tttttgaaat tgttccttgt gactcggagc tgaacggcag
      301 gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa taagttcaac
      361 caaacgaaaa ttatgaggag ctatttttgt ttggctgttc ttattacttg atgcaggaca
      421 gctttttcac aacctctttt cgtttagcac taaatttcct taaaaagtag aaaagctttc
      481 gaagcctcgt ttgtttcttt tactgtataa taaaagaaaa gagtaacaat cgctaaaaga
      541 tataaacaag agaaaggaaa tacaagatga ttggtctgat ttattttgtg gtgatcgttc
      601 tggcgaacac agttggcgct atttccggga tgggtggagg cgtgttgatc aaaccgattc
      661 ttgatcttat cggcgcccat tctgttgcag ggatttcttt ttactcaaca gttgcggtat
      721 tcacgatgtc cattgtttcg actgtgcgac aagtatcaag cgggaaatca ctgaattggc
      781 agattgtcgg ttgggtatct agtggagctg ttgttggagg agtagtcggc aacattgtat
      841 tcgaagtttt tttacagtta tttgaaaacg agaagcatgt gcagctgatc caaatttttt
      901 tgacggtatt gaccttggtt tttgcgttct tctacaccaa gcatcatcag ccaaaatttc
      961 atttgacgtc atggacttgg taccttatat gtgggggggg ttctgggctt tttagctagt
     1021 tttttaggta ttggtggtgg accagtcaat gtttctttgt tgatgctgat gtttgcattg
     1081 ccaataaaag aagcaacact atattctcta tctacgattt tcttttctca actggcaaaa
     1141 ttggtaacga ttgctttaac aagtagcttt atgcgctttg atttatctat gttgttttat
     1201 gttatccccg ctgctgttat tggaggtttg tggggagcaa gatttagcag ggttttgtct
     1261 ccaaaaaaag tgacctttat ttttcaagta attgtcctcg tggttctttt ggtcaattgt
     1321 tacaatgcct ttgtcatctt acaaacatta ggagaatcta gactaacgca agtcaaaatc
     1381 actgaaacat attgttctaa atgaaaaaaa gagtatacta tgataaaaaa attcatttta
     1441 aaaaatcaga aattggctgg tgatttggat gttacgggaa atgcgtttag aagatgcaga
     1501 agcactacgt gaaatcaatg cggatcaatt agggtatgat ataccattaa ccgttacggt
     1561 gagacaaatg aagaaactgc tgaaggagcc agagaaaaat tttttcttgg tttatgaaga
     1621 tcctacttct aaaaaagcag ctggctatgt acaagcggag tcctatgaat cgatttttag
     1681 cgatccaatg ttcaatatta tggcacttgc tgtgtctaaa tatgctgaaa aaagaggcat
     1741 cggaaaggct ttgatgactg gcgtagaaga agaagcgaaa aaacgaggaa taacgacaat
     1801 aaggcttaat tctgcagaat acagagtaga agcacaccaa ttttatgaac acatcggtta
     1861 tcattccgat aaaatgcaaa aaagattttt gaaaatcctg taaatcttta tcaaaaaaat
     1921 acccgatcac caagccgata cttgatgatc aggtattttt gtttttttga ctaagaaagt
     1981 aattgcatca agaaaccaga gatgatcaaa acgatggcac caccaagtcg attgcccatt
     2041 tgagcaaaag caataagttc catccgatta gctgcagata agacggctac atttcctgtt
     2101 ccgcccatcg agttgttgca taagccagca gtaatagttg attcaattgg atataaacca
     2161 aacaaacgac cgacaaatcc tgaaacaact gaaatcgcga taacacttgt cagacataag
     2221 acaacaaatt gccatgtcaa agcagaagct aatacattca gattcagcaa cgcaataccg
     2281 ataccggcaa gaacagctgc tgtcaagttt ttgacaatca agttattgaa catgatagct
     2341 gcatcttcat aatactctgg taataggttg gcgattttac agatgacaac gataataatc
     2401 ataaatgcgt aagcatggac tttaggaaca aaatagttac caatttgtcc taagatgaag
     2461 aaagtcaatg ataccatcaa accaacacct aactgtggaa tggaaggttt cacagttcga
     2521 ggttttccat cgccaagatc gcctttttcc attaatttac cattgccgtt gaattttggc
     2581 attgattgtc cagcacgagc aactaatgct gcaccgatga tagctaagac atttgtcatt
     2641 gctgtcgcag gaatcaggcg ggaaatgata gaagaagcat ccgtacctaa aacatttgca
     2701 taaatcgttg ataaaggaac ggctccggct ccaacaccac cagccatcgc aggtaatgag
     2761 atgtataaaa cggaatttgc aaaaccattt ccgataagca tgcccacgag tcctacggca
     2821 aaaaaggaga cgaccattga aataagtgca acgggaataa aacgaacaga agctttcatc
     2881 aatagattac ggttcatacc aaggatgcta ccagtaatca aggcagcaat ataaaagtcc
     2941 caaaagccca tattgctgac aaagttttct gtggatttca ctacatattc aggtaagatc
     3001 ccaaatgtag caatcgcagc agaagcgaag atcgcaaaaa cggaaccacc acccaaataa
     3061 cttttgacga tagggatttt tgtcccgata aaatgaaata aattaccgaa aataaccaaa
     3121 actgcgattg gtccaatcat attcgtcggt aatttcccta aagccattgc aatgatcagg
     3181 acaaggacca tgatcaaata aagtggcaaa cttacgccgg tgatttttgt ggaccaaaaa
     3241 ccttcttttt tcttttcctc aactgcagtt gcagttgttg ttttttgtgc catttttaca
     3301 gatcctttct tattctgtat catatgaaaa aagccaaaga aaagcgacaa aagagcagga
     3361 gttggctggc cgctgttttg ccgcagtcgc ttttttcttt ttagacgtca tacaaagttt
     3421 ctgtatgttc ttttactata acgatcttaa tcttctaaag atttatattc aggaacccat
     3481 ttagcagatt caactgcttc tttgatatcc gtgatttctt cacgattcaa gttttgtgcg
     3541 acaactgatt ttgcgacagt ttcagcaatc gtttgagaaa attcagtgat ttttgctact
     3601 ggaggcaaga tagctgctcc tggttttgtt acatcaacga ttccgcctaa tgctcgacta
     3661 gcttgagaaa tgatttcagc atttactcgt gtagctgttg aagcaatcaa accaagtcct
     3721 aaacctgggt acatcaaagc attatttgct tgaccgattt ggtaagttac accattatat
     3781 tcaacatcag cagcagggat acctgttcca actaatgctt ttccgtccgt ccattctatt
     3841 aaatcttttg cttttgcttc tgccaatttt gttggattag acaaaggcat gatgatcgga
     3901 cgtggtgtat gagcagccat ttctttcacg atttcttcag tgaatgctcc aggttgggta
     3961 gaagtaccaa tcatgattgt cggatggatc gcttttacaa ctgcttctaa gtttgtcaat
     4021 tcatcagcat tagtgaaatc tgagcgtttg cgagcgaatg ggatttgacc aggagtcaat
     4081 ccttctgtat cttcaaacaa taaaccttgt ttatctactt gatagaaatg ttggcgtgct
     4141 tcttcttcag gtacaccttg acggatcatc tcatctaaaa ggatgtttgc gatacccaca
     4201 ccagcagtac cagcaccaaa tgtcaaaatt atttgatctt tcaattgttc accggatata
     4261 ttcaatccgc ctaatacacc tgctaggaca acaattcctg taccttggat atcgtcgttg
     4321 aaagtagtga tttgatcttc gtatttttct aagatcgctg cagcatttcc tcgtccaaaa
     4381 tcttcccaat gcaataacaa ttctgggaat aattcggtag ctgattgaac gaattgatcg
     4441 atgaattcat agtaagtttc gccttctaca cgagcatgac gattgcctaa gtataaggga
     4501 ttattcaata attcttcatt attagtacca gcgtcgatcg atacaggcaa aacttgtgct
     4561 ggattaatac cagcagcagc agtatagacc atcaatttgc cgatagcgat gtcaacaccg
     4621 ttcacacccc agtcgcccat acctaagatt ccttctgcat cagtcacaac aatcaaacga
     4681 atgtctcgtc catctgccgc gttcttcaaa cttgctttga tgttttctgg ttcgtcgatt
     4741 gaaagaaatg cagcatcttg cggtttcaag aaaatttcgt tgtattgttc gattgaatcc
     4801 gcaacgacag gatcataaac gacaggcatg aattcaacta aatgttcgcc catcaattta
     4861 taaaacaatg tccgattcgt attgaataga ttcattaaga agatacgttt ttccaaatct
     4921 gtttgtttgc ttaagtattg accataagct tgaacagatt gttgttccag tgtttggaca
     4981 gtacttggaa gcataccagt gattccatat tttgcccgtt cttcttttgt aaaagctgtt
     5041 cctttattta gaaaaggatt atttaataag tttaaccctg taagcatgaa tgattcctcc
     5101 taagttttaa tacaaacgaa gtatagaagt cagatgatga tatagtcaaa aagagcaggt
     5161 atgataaatt ttatttatat gtaatatagc caaaagaaat caaaatagca gtatttgttc
     5221 tttttatatc gacaatcctt gcttgaatac tatgcgtatc tcttatatgc tagagaaaga
     5281 aaagtagaag aataagggat agatggaatg gataaatagg atttctgagg aaaaaagcac
     5341 atgatagcgt tgataggagt cacctatgtc aggtgataaa aatacataag tagaaaatta
     5401 agaggaggaa gagaaaatga aagaagttca attgattaga aaaagcgaac tatcagaagg
     5461 cggttgcaat gcatgtggcg tagtagaagc gacaagctat acattaaaat tagattccaa
     5521 tcaagcaata atctctgaac tgacagtagg cggtcttgtg gacagtctgg cgcttgctga
     5581 aggttttact ggagaagata tttatgagat gttttcagaa gtccgtcagt taaaaaaagg
     5641 agaaaaatgc atagaggttc atcatgagag cccgaatgtg aggttcaaac gtggtgataa
     5701 tgagatgatc ttcaagaatc atgtgtctga tcacactgaa ctatatgaaa tcgtgaatca
     5761 aatactgtct gggatttttg aattaggacc ttatgagttc aaggaagaaa acggtaatcc
     5821 aaaattaaat gaagaatggc aagaaacgat tgaaacacaa agaaacaatc cacacttatt
     5881 tcaatagtct tccacagtat ttcgtaaaaa tgttgatgaa aaagaaacac cgtgaatcag
     5941 aggtcgtgaa aaacctgccc tgcatcaagt aataagaaca gccaaacaaa aatagctcct
     6001 cctatttttc gtttggttgg gcttattacc gcagatgcaa gtttttgaac accgtttaag
     6061 gaagagactg tgaagtcaac gttctgacct gagtattaga gagacaaatg gcaaaatagt
     6121 tcctcatatt ttgccatttt ttgagctaat accgagggtc agctgattga acaccgtttt
     6181 atcgagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaaaaattt tgtgaaaatg
     6241 gctttccaca tttttgcata aatttgactt aataccgaga aaatggcttt tgaacaccgt
     6301 ttattcaaaa aaagggactt tgacactact tttgtcaaag tcccatcggt tttttatcta
     6361 tttttattac aatcatttct tccagagtag tgacatctgg aatggctgaa atgtaaacag
     6421 attatttgcc tgtatcaatg tcgaagagta atataaaaag tgtaaacgct atcatattgt
     6481 tcatcattca aattttatag agagaaggca ttgagatgga aaaaacatat caagcaggta
     6541 caaatgaagg aatagtagat tttatcaata tggaagattt agaaatcgcg gcttcacaag
     6601 tgattccagc tggtggttat ggttacatca gcagtggtgc tggcgactta tttacttatc
     6661 aagaaaatga acgggcattc aatcatcgtt tgattattcc tcatgtatta agagatgtag
     6721 aattacccga tacaactaca cattttgacg aagaaatgct gactgcacca atcatcatgg
     6781 caccagttgc tgctcatgga ttagctcatg tgaaggcgga gaaagcttct gcaaaaggtg
     6841 tagcagattt tgggacgatc tatacagcaa gttcttacgc ttcttgtact ttagaagaga
     6901 taagagaagc aggcggggag aaagcaccgc aatggtttca attttatatg agcaaagata
     6961 atggaatcaa tttggatatt cttgaagtgg ccaagcgtaa tggagcaaaa gcaatcgtat
     7021 tgacagcgga tgcaacagtt ggcggaaacc gtgaaacaga ccgcagaaac gggtttacct
     7081 tcccgctgcc aatgccgatc gttcaggctt atcaatcagg agtaggccaa acgatggatg
     7141 ctgtatataa atcttccaaa caaaaactta gtccaaaaga tgtagaattt atcgctgctc
     7201 attctgattt gccagtctac gtgaaaggtg ttcaatcaga agaagatgtc tatcgatcat
     7261 tagaatctgg tgctggtgga atttgggtat ctaaccatgg tggccgtcaa ttagatgggg
     7321 ggcctgctgc gtttgattcg ttgcaatatg tggcagaagc agtagataaa cgtgtaccaa
     7381 tcgtgtttga cagcggtgta cgacgtggac aacatgtttt caaagcaatc gcatcaggtg
     7441 ccgatcttgt tgctattggc agacccgtta tttatggatt gtctttagga ggaagtacag
     7501 gagtccatca agtgtttgat ttctttaaaa cagaattgga aatggtcatg caattagctg
     7561 gaacacaaac agtagaagat atcaaaaaaa taaaattgcg tgagaatcgt tttatttaag
     7621 aaataaatga cgaaaaaaat aggagtgcgg cagatgatgc cgtactccta tttttatagt
     7681 tcaaggatgt cactaataga ataatttgaa acgaaaaaat aaaattgaaa tattttttca
     7741 tacttaataa aaaaggaaga atattcatgt aaacgatttt attgtttttt tctcttttat
     7801 acaaaaaggg tcataatttc aagtaagcgg atgaattcat gaggattttt tgcatactgt
     7861 ctctactata cttaggtcac taagaaaaag gagtgattta ttatgaaaaa gaacatgatt
     7921 gggacattat tactttcaac acttcttcta ggcggcttgg caacaccagc ttttgctgag
     7981 ggtcaagcga cttcaaaagg agatattacc tttactgaac caacaaatac cgttgagcct
     8041 cttaacccta ctgatccttc aaaaccagta gagccagcag atccggaaaa tccagcaact
     8101 ggccagacag gttctttaac attggatgta gttcctgaat tgccatttgg tacacacgag
     8161 attgaaagcg gaacaaaaac gtatcaagta gacgcttcaa aaaacgatac accttatctc
     8221 caagtatcag atcgtcgagg tgtaggtgca gatggccaag cacaagggtg gaacgtgaca
     8281 gtttcggttt cagattttgt aaacggttca caagttttgc aaggcgctga attagatttt
     8341 ggaacatcca ctgttaaaag tacgtcagat aacgaatcca ctggaccaac tagtcaaact
     8401 gtaactgggt tgagtaaagc aagtgcagcg acaccaatat ttacagcagc aaaagatcaa
     8461 ggtctgggaa catggctttc tgtttatgat ccagcaaata tcacactgaa agtgccgaaa
     8521 gcagcagctg gaacattcac ggctgatttg acttggaacc tcgtggctgg cccagtagca
     8581 tagagtatga aataaattca acaaactgcg gtgaaaatac tataggtgaa ggatttttta
     8641 ccgcagttgt tgttgaaagg atgtggtgga caatgaatca aagtaaacga ctgtttgttt
     8701 cgtttttttc tattttaagt ctatttttca tttttccgtc aatcagccaa gcaaaggatt
     8761 cagcaggaga ctttggaatc aaaccggtat ttccagagaa ccaaatagat aaagcaatcg
     8821 gttattttga tttattagtg gcaccggaac aaaatcagac actagaagta attatcagca
     8881 attcttcaga tgaagaacga acatttgagg tctctgtcaa tccggcggta acgagtgatg
     8941 gtggaacgat cgattatagt caaaaaaacc caacgttaga tgagacattg ccgtttgatg
     9001 tccgtgacgt tttgctcatt gcgaaaaagg aaatcaatgt atcagcacat gcagaaacaa
     9061 ctatacctat agaagtgaaa atcccagcaa aaagctttaa gggacgtgta ttagcgggta
     9121 ttcatgtttc acctaaagaa gaagctgaaa cagaaaatgc tacagaagga gcacaaatca
     9181 aaaatcgtat tgcttataat ctcgcagtcg ttttacaaga aagtcaagag acgatagaac
     9241 cggatctgaa acttttatca ggagatttgg acgaggtcaa cgcaaagcca actgttcagc
     9301 ttcgtttcca aaatcctcag cctaggatta tcagcaattt gatatttacg agcaagatct
     9361 tttatgaaaa tcagctttat attgaaaata cgtctaatga atttttagtt gcaccaaatt
     9421 cgaattttca cttgaatctt gatttagctg gtgataaagc aaaagccggc gattaccgcg
     9481 cagaaatcat agcaaaatca ggcgacagta atgaatggcg atttacacaa aactttacga
     9541 tcaaaaaaga aaaagcacaa aaagtaaatg aaaattccgt ttttgcagta caagaacaat
     9601 ctttcccatg gctttatgca ggattggttg ctgcagttct agtgttatgg ttgattttgc
     9661 tgtttttgtg ggtccacaag aaaaagaaaa aaggagaaac aacatgatga ggtgatttcg
     9721 ttgaaaacat cgatcttatt gggagggatt attgcaagtt tgctgttctt cagctggcaa
     9781 gagccagtcc aagcagatac caaaagttca ggtacgatta cattttatga tctaagtcca
     9841 gttccgcatc caagtggttc agtcaatggc gcatcaaaac agcagacaca gctaccgatt
     9901 tataaagaat ctaatcaaca tggcgtattc ccggagacga atgaagaaaa gatccgtttt
     9961 ctttatttga tcattggtat agaattatgg cttcttgcgt ggttgttgtt atggagaaga
    10021 aaggaaaagg aggatgaaca tgttgctaca aaataaattt tcattttttc gctccttctt
    10081 ttttacttcg ctttttgttt taagtatatt tatcttttca ccttcggcag aagctgttgg
    10141 agcaacgcct cctgccattt cttatgatcc atacacgtat gaaaatatcg tacctccaaa
    10201 tggtggacgt agtgcagtca atggcgggaa gcttgtccaa ctaactgggg ctaaccaatt
    10261 gcctaaattg actgctactt atttgaacga tggttcaaat gatccttgga aaggtggcac
    10321 ggtccgtttt cattgggaaa gccatgtgaa actaacaaat gtacttgatt tggtgaatat
    10381 cactgcacaa ggatttgcat ttgtggcgat catcaatttg ccggaacatg tatcaagcag
    10441 tgatgtatta aaagcaattg actggaacaa cgcctacttg tatattgccg gtgcccccat
    10501 caaggttact agcggtagtt tattatcagc aggaagccat accttgcgtt ttgggatggg
    10561 gtcgtgggat acaaatactc tattggcaga tattattgct attattacgc gaggcgaatt
    10621 caacgtggac gatatcccgc ttcaatttga tgttacagct aatgtcgcga agatgacaga
    10681 aaacggagat aaatatgata cgagtccaaa caaaattttg accaagggta aattacaacc
    10741 ttctatgtct aaaaaggccg ttttttctgt tgatttttat gattcgaata atattatcca
    10801 agatcgtatg ttcaattcga ttttagctcc agctgcaggt agattttatc ctaaattaac
    10861 tggcggaaac ttgaatccag gagcatatgc attttctgca agtgcagaga taaatacgtg
    10921 gaatagttac atctcaacaa gtgattcaac aggaaaatat aatacatttg ctgataccac
    10981 agaatacgtg gatggaaata accagaacct tccaggctct accaatagtc ggacattgac
    11041 gatggggctg actgaaggac gttttgatca gttgccattt agtcgctttg atcgcgtagt
    11101 caactatttt acaggaaaaa acgttacaca aggagcaata ctgactcata ctccagcaag
    11161 cacaagtgga ttgaatcaga cagccaatat cgtgtacagt gggaaagatg cgcaaggcgg
    11221 attgctttct cctgttgctt taaaggtatt ggagcagtac aagctagacg gaacggtttt
    11281 accaacaaac ttaacagccg atagtcagtg gggaaagctc ctttcttctg catcgtacca
    11341 agtcacggcc aattggtcag caactgaact aaaaaagggt tcgatccatt acaggcttta
    11401 ttcgaaagga acccaaaaat tagcgacagg atggacagat caacttttcc aaacgatcca
    11461 atcaaataac gggggtgtaa atacagctac ttctacacta cctgcattgg cttcagggca
    11521 atattatttt gattatcgct tggtggacga tgtacttact aacatgtatc cgtcttttgc
    11581 gtacaaatgg cagtctgaac aagctggaat caccactctt cctcaaataa cagtggctaa
    11641 ttttccatct gtgagtgcag atagtcagct aaaaaacctt agcagacaac ctgagacagg
    11701 agatcccctg tctgctctat caggagatac gattcaagaa aatgtgacat tcacgttaac
    11761 aaaatctggg gatgcgataa ccgacaaaaa aatcacgatc tcacttcctg aaaatactac
    11821 ttatatcaat ggttccttaa aattaaatgg tacaactatc gcagataccg gcattcaaca
    11881 aggagtttct gtcccagcag atctattgag caaaatagga gataccattc atcttactta
    11941 caactatcaa ttaaatactg tagatacttc tgtacaatcc gtcagcatac tgacaaaggc
    12001 agctgtactt tccagcaata ttacattagc agatggtgct aagcttccta acccagttgt
    12061 ccaaacatct gctaaaacaa ttcttgtccc caaacaagaa ttaacattag ttaatgtacc
    12121 agatgatttt acatttggta acgatctccc caaaccgttg aagacaagtt attatgaagc
    12181 aaaaggcgat ttttcttttg atgtgcggga tactcggctt ccaagcacgt ctccttggca
    12241 actgacaggg acattgactt ctttatttaa gaatgatcaa ggtcaagaat taagcggaac
    12301 aaaattatac ttcaatcatt ctggatcaaa acaattgatt cagcaaggtc aaaacacgtt
    12361 gatttatgag agtgatggaa cagctaaagg agaggttctc gttgactttc ccgataccga
    12421 tggtttactc ttagaggtaa atagcagtac gaatgctcaa cctggcgcaa cgtatcaagg
    12481 catggtcaca tgggaactga cagctgggcc aaccagctaa gtgattagaa tttgtatgga
    12541 tctgtgataa ctgtcacaga tcctcttttt caaggatgag aaggaaactt ctgctgttaa
    12601 aatcagaaat gcttggagga gtggacttgg aacttttcag tttgcttgaa aaaaatgaac
    12661 aacggcaaat caaacttttc caacttttat tagaggaaaa aggagagatt tccgttaata
    12721 acgtacttac ccgcttatca gttgatcggg ctaccttgaa agaagatctg ttttatttac
    12781 aagctaactt ggaaaatatc aaacaattgg aaattgttat ccaagaaggg aatattttat
    12841 taatcagaca tggaaatata gcagcagtcg atgtttatta tcattgctac ttgcgctatt
    12901 ctacaaaata tcagatactt ctttatctgt tggaaaatgg ctcatttgat cggcagaaac
    12961 ttgtcatgga acttaatatc agttcggcta ctttgtctcg cagagtgaag gaattaaatg
    13021 attctttaaa tgagtttcat ctgaaaataa aaagcgggcg gctgaccggt ccagaaagtc
    13081 aaatttgtta tttttatttc caattatttt ggtttggacg tccttatctt gaaaacaaaa
    13141 aagaatacag tgatttatca ggaaacactt ttttagatct gctgcaaaaa gagtttagtt
    13201 ttactattac agaagaagga cgattgaaat ttgaaatatg gctttccatt atgaagaagc
    13261 gaatgcgctg tacgaaaaaa tccaaaccag attatagcaa tcttcctgtt ggattgtttc
    13321 aaacgacaca atttttattg gacctccata catttttgac ccgttacttc tttcaatatg
    13381 cctttccttg gaatcaaaat gaaacgttgg ttttttatct tttcatgatc agtaacttta
    13441 cattagatac acataactcg catgtccagg agtttttgat aagcgaaaaa tacaaagacg
    13501 acgtagtagc tcaattgaat cgaacgttta aacaatcgat agaagaatat ttctcttttt
    13561 atgtttttcc agaagagatt tcgcaaaaga ttgcgttgac agtgatgcaa aatcacgctc
    13621 attttgccta ttttagagga tggatgaata tttttggcca agaaggatta aaacagcggt
    13681 taactgaagt agaagataaa caattcctgg ttttatgtga aagattggtc agacaaacat
    13741 ttgctattgt tgattttact gaagtagacg aggaaaatgc ccgctttgaa ttatttgctc
    13801 gctatgcatc tattttgcgg acgctgatca agcagttaga attacgtctg caaattgctt
    13861 atgatcttcc ttatgaaagg ctaatggcgg atatcgtgaa agaaatcatc caggaacaat
    13921 tgctgaatac gatcaaatat gatttagtcg aatacgagaa agaaaagcag tatgatatca
    13981 ttttgactag ccaattgaaa gagtacccgt cacagaagtc cgctaaagtt tttgtattca
    14041 ctagcaacga atataattat gattgcccct atttgaacca atttttaaaa gaatgttatt
    14101 tagaaaaatt gaatttaagg atgaataaaa catgaaaatc gtgatcattg gtgcttccca
    14161 tgcaggcatc acagctgccc taaatctaag aaaacttcaa ccgaaagcag aagttttact
    14221 gatcgatgaa gatcataaag atggactagg ctatgtttca aatggaatca acctttattt
    14281 aaaaggaaag atacgtagtt tagctgaagt tgcccataac atgcggactc ttcaagaaag
    14341 tggggcaaaa ctgatcaccg aatggagagt aactgaactt gatccagaca aacatcagct
    14401 catcctcgca tccaaagaag gaaaaaacga gaccataacc tatgataaat taatcgtagc
    14461 aacaggttcc tctcctgtca ccttgtataa acaaattgaa gctgaaaatg tctacaccta
    14521 taaaaatcta gtgcaatcta agcaagtact tgccgcttta aaagaagcaa aagaagtagt
    14581 gatcttcggt gctggttata ttggattgga attagcagat gctttgagaa ataaaggata
    14641 cattattcat ctagttgatt atatgccgaa tgttttatca agatatttcg ataaggatat
    14701 gatcaacagt tttcaaaatc agctgcaaac taagcaaatc aatttttatc caaatgaatt
    14761 cttgatcgat tggaaaaaat cagaagagaa agtagtatcc gtacaacttc tatctcaagc
    14821 aataaaggca gatatggtca ttttttctgc ccaaactcgt cctaatacga cattgttaaa
    14881 agaaaaagtg gctttgtatg aggatgagac tgtgatagtc aatgaatatc tgcagacaag
    14941 tgatccggac atctatgcga ttggagatat cgtcccagtc agttttgaca aaaataaacg
    15001 gcatctcttt ttacctttag ttactcgagc tgtacatatg gcaagagcag tagcgctaac
    15061 tttgagtggt cagccaaccg cttacgacct cagacaaaag ataacagcag cagtcattat
    15121 agactacttt ttaggaactg tagggctgac agaagatgaa gcgccattct tggaacaaaa
    15181 taccggttcg tgttcaggag aatttgatct atttccacaa tacgatgaag aaaacaaaac
    15241 agtaaatgca aagctgatct accatccgga tacattagaa ataattggcg gacagctgat
    15301 aagccaagaa ttcttattat cagatttgaa cctgcttgcg gatatcgtga aacacaaaac
    15361 tacgattcca cagctagcag ttgaggattt tggatttctg tctgaacaca cctctcgttt
    15421 tcactacctc aatgagttag cttttaaagt actagctaag agagcaaata gcaatagagt
    15481 agataaaaga ctgtgagaaa tatcacggtc ttttattgtg tggggatata ataaatatta
    15541 atcaatcgag aataaaaaat tcatataatg aaatagcgga agaatttttt gagcataata
    15601 ttcgccaaaa atttatcaag tgataaaatt gttgtataaa atgattattt caggaggagg
    15661 agttaaatgt ttttagcatt aaatgaaatt atgcattcaa agttacgtta tgcgttagtt
    15721 gcaggggtga tgtttttgat cgcctacctg gtattttttt tgactgggtt agcttatggc
    15781 ttagctcaag ataatcgaac agcagtcgac aagtgggagg cagattcgat tgtgctttcg
    15841 aaagatgcaa attcaaatct agggatgtcc atgattacta agaagatagc agaagaagtc
    15901 gaaggtggga aagtggcgta tcttgcccag acacctggag tcgtgactag taaagacagt
    15961 acagaagaag gaaaaatcaa tgttagcttc ttcggtatcg ataaaaatca attcatcatg
    16021 cccaatcttg ttgaaggaaa agcttttgat aacgatgatg aggcagtagg cgatattagt
    16081 ttgaaagaag aatatggtct agccgtagga gatacagtta agttatctgg tagtgacaag
    16141 acgtttaaac taactggctt tacggatcat gccaaattca atgtctcacc agttctctat
    16201 acaaccatca atgcttacca agagatccgc ttcgaaaaag aagatacaag tgaaaatgca
    16261 cggattaacg caatagttgt cagaggtaaa atcagtaatt tacctgaaga tttagaacag
    16321 atcaaaattt ctaaattcat caatgaatta cctggatata atgcgcaagt attgactttt
    16381 ggatttatga tcggattttt gatcgttatt gcggctattg tgatcgggat tttcatttat
    16441 gtactgacga tgcaaaagat taatatcttt ggtgtaatga aagcacaagg gatcacagga
    16501 ggatttattg cgcgttctgt agtggcacaa acattcattt tatcatttgt tggtattctt
    16561 cttggtctat taggaacagt gggaacatcc cttgttctac ctgatgcggt tccattccaa
    16621 agcaattggc tgttttttgg cgtcattagc ttgttgatgc tggtagtagc tgtgttaggc
    16681 gcactatttt ctgttcggac aatcgtaaaa atcgatccgt tgaaggcaat tggataagaa
    16741 ggaggaagag catgaaagca atagaattta aagaagtaga aaaaagtttc caagatggcg
    16801 accaagtagt taaagcattg aaagaaacaa ccatgagtat cgataaaggg gaatttgttg
    16861 caatcatcgg tccttctggt tcaggtaaaa gtactttttt gactttggca ggcggattgc
    16921 agacgcctag cagtggctct gtatttatca atgatcaaga attcagtaat caaaaagaaa
    16981 gtgaacgcgt taaactacgt tttaaagaaa ttggctttat tttgcaagct tccaatctgg
    17041 ttccttttct gacagtaaaa aagcagttat tattagtaga taagatcaaa catgaacatc
    17101 gtcaaaaaga agcaaaagaa ttatttgagc agcttggcgt ggataagttg atcaacaagt
    17161 atccagaaga actatctggt ggagaacggc aaagggtcgc aatcgctcgt gcattgtaca
    17221 atgatccgtc cattattcta gcagatgaac caactgctag tttggattca gaaaaggcac
    17281 gagaagtagt cgatatcttg gcaaaagaat caaaagacaa aaataaagca acgatcatgg
    17341 ttactcacga tacacggctg attgacaaat gcgatagaat atttatgatg gaagatggtg
    17401 tattgaaaga aaaagaataa ccaaaaagca ggtggaaaaa tggtccatcg atacaacaaa
    17461 gatacacagc aaaaaatttt gaaagaagcg actgacttgt ttatgtccaa gggatatctc
    17521 ggcacttcaa caagagagat cgcacaaaaa gttgggatca cccaacccaa tctctatcat
    17581 tatttcggtg ataaagaaaa actatatacg gcagttttag aaaaccactt aaaagatgtt
    17641 ggaaaagctt tgagagaaat cgttcaaaca agcgaaacgg gatttcaagt aaccttgaca
    17701 aaaatggcgc agtttttgat tgagacacat ctggtcgatt tgtttctgat gcttcacgat
    17761 cttgaaagca atctttcaaa ggaaacaaga gatcatcttt tctttctatg gaagaaaaat
    17821 tatcgtgaac cttttgaaga aatcttttca gcaaaccaat ctgtgctaag aaatgggatt
    17881 tcaaaagaaa tcgcagcaag gcattttttc ttagtgttag caccttacat cactcggtcg
    17941 aaaagacctg tggaagaacc gctaaatgtg gcgcaattga tcgatttata tttgaatggt
    18001 gtattaggag aagcgtaaag ctgttatata gggagaaaaa tagttaggtg aagaagcaag
    18061 atatgacgaa gatcgttata tcttgttttt tttatatagg tagtttttca taaatacaaa
    18121 tttgttattc tctcatgatt atcagggtat atttttattt attatgtcgg tgttccagga
    18181 agtgttttaa tttaataacg aagaaattaa ttgtttgtag aatgaaggaa actatttatt
    18241 gggtagatga atcaccacga atagtcaaaa ggagatttca ggatatgcca aaaatgaaaa
    18301 aaatcatttt agcagtaatg ttattttcta cggttatatc ttttacagcc ccttttttgc
    18361 gtacttaagc tgattttaag ttttgaccct tagtctggat ctacgaggtg agagtcaaga
    18421 atgaaaaaaa taattggagc ttttgtattt gcaatcatta tcattattat ctgttttcaa
    18481 atagatgttt ttggtcaaaa aaagatagtt ggacaaacgg tcagcagtac gaacagtacg
    18541 gttacaaaga caaaggaatc atttgatgat acagaacaag agacattaat caaaatcgga
    18601 aaaaatgacc gtacactata tacagatgac tcgctaacta ccccacttgc aactataaac
    18661 ggcggagagc tggcggagtt tctttctgaa acaaaaaatg cttatcagat caagacaaat
    18721 gatggctata ctggatattt agccctcgaa gatggaacca aagtcacaaa gaatatacaa
    18781 acaaaaccgg aagagctatc tgatgcagtg attgtattag atcctgggca tggcggaaat
    18841 gatactgggg cattaagcaa tgatgaacaa acagaagaaa aagatataac attaagtaca
    18901 gccataaaag taaaaaaggc gctagaggat gctggagcaa cggtttattt gacccatact
    18961 acagatgaat tagtacagct tggagatata tgcgactaca gtgaagaaaa aaaggcagat
    19021 gtctttatca gtttacacgc agactctacg gaatatgcga atgaagcaac tggaatcacg
    19081 acatattatt actatggaca agaggaaaca ttagcacaga caattgctga ctccttcact
    19141 gatctgcctc tggattcaag aggaatctcc actgggaact atcaagttct tcgagaaaat
    19201 cttcaacctt ctattttaat cgagatgggt tatatgaata atgatagtga tttaaaggaa
    19261 ttagtaacag acagttatca gcagcagatt gctgcaagtt tgacacaagc attgaccact
    19321 tattttcagt aataaataag cccagccaaa caaaaatagc tcctcatatt tttcgtttgg
    19381 ccgggcttat tatcgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgagcc
    19441 tgccgtttaa ctccgcgtca caaggagcaa tttcaaaaaa cagcttctca tgtttgatga
    19501 ctattgtgac ttgtgaccga ggagttggca tgagaacacc gtttattcgg aacaagggac
    19561 tgtgacatga cttttgtcac agttccatta tttttttagc agcttcaaag ctttttctgt
    19621 attggcaaaa tgaagtttgg catatttttc aagcataggc atgccgcctt tctccaatag
    19681 ttttgccgct acttggtcag atttcgttcc agctcctgat ggaagggtga ttccagcttc
    19741 agctgatgct ttgtgtaact cttccaaaaa gctggcacga ctgatgatag aagcagcagc
    19801 aacagctaaa tgatattgtt ctcctttagt aacgaagtac agtttttctt gtaattgatt
    19861 tttttctaaa cgcgcatatt ttttaaaatt tgcttccggc gtaaactgat cgatcaaaat
    19921 cgcttctggt ttttctggct cgattttttt tagcagcaaa tggatcgctt gattgtgcaa
    19981 agcgactttc atccgaacgg cattgtattt tggttggatc tcattgtatt tttctggaga
    20041 taagacaagc agtttatagg ggatggtttt ttttaagatg tcagcaagct ggatgatttg
    20101 gctatctttt aactcttttg aatctctaac accaagttta cgtaattctg caagctgatc
    20161 acgtttcaca taagcagcac atactgttag cggaccaaaa tagctgccat ttccgacctc
    20221 atcactacct aaaacggata attgactgat attttttggc aaagaagagg ctgtttttgc
    20281 tggcttggct tgtttatcct gtgcctcccc ccatttatca gcttctattg ctgcattcgt
    20341 accttgaaac atcacttttc cggatgtata agcggtaatg gtcgtcccgt ctttttttgc
    20401 agcaaagcta gtataaggaa tctgtttatt taacaaataa gagcgatagt atgctttcat
    20461 ttgactaagt tggtcgtttg ttgctttgat aatgacgttg ctcataaaat tctcctcgtt
    20521 tttatacata aaactatacc gcaactcacg acttttcgtt ttctaagaga ttgcgagaca
    20581 tagcattttt ttaaagttta tcatataata gttaagtatg tgaaaaatat ttgtggagaa
    20641 cggtgaaaga accaacttca tggtacacta aataaaactt tcatcaaata ggaggaaatg
    20701 attcgtatgg cgcacgaaaa aacacgctac aaagcagtga ttgccaacca aacatatact
    20761 attatcggac gggaaaccaa gcatcatatg gatatcgtaa cgaagctgat caatgaacag
    20821 ttagcagaat tgaaacaatt atcaccacaa atggataatg aacaagcagc aatcttgatg
    20881 gctgtcaatg cgctttcgga tcaattgaag aaacaagaaa gaatcttgga gctggaagaa
    20941 gaaacagcag aattaaagaa aaaaatgatc aaatttacag agcttgaaaa tcgtgtgaaa
    21001 cgcattgaag caatcgagaa cgaagctcgg gaagtattga aagaaaatgg tcaagcagat
    21061 cataccatta aaaatcatgt ggaagcacaa cagattttaa acgagaaacg aaaaggacaa
    21121 attaaacaaa aatcttctaa ttaagatcct tttatcaaaa taagaaaaat ttaaaaaagt
    21181 tagaagagaa aggaacgtag catgctgagt ttgctgattt tatttttatt gctaattgcg
    21241 tttttttctg gagcacgtcg cgggtttgct ttgcaaatca tattcgcgat tggatatgta
    21301 ttgtcattta ttgctgccca gcatttttac aaaccattag caagtcattt ggaattatac
    21361 attccatacc cagctgtcac cccaacatca aagttagcat tttttgatca ggtatttgct
    21421 tttcacctag atgaagcttt ttatgcagga actgcttttc tgattatttt gctaattggc
    21481 tggctgctta cccgatttgt gggggttttt gtccatggat tgacgtatgt gccgatcttg
    21541 agacaggtag actggattgc aggaggcatc ctgtcactaa tcatggctta cgttactata
    21601 tttttgattt tgcagttact tgcctttgtt ccattagatg tggtccaaaa tcaattcggc
    21661 gagcatagtt tcgctcgttt tatcgttgag agaacgccat ttcttaccaa caagatgtat
    21721 gagctttggt aacgaatatt ttgaattaag gaaaatagtg ctggaaaata tttcgttttt
    21781 tgtacagagt agtttattta agaaggctgc gtagcgtatg tccggcacag ctcttttttc
    21841 atagtgaaag ataaggtgat aaaatgaata accgaatcct tgaaacattg gaatttgaaa
    21901 aagtcaaaca gatggtcaga caatttgtag tgactgcaca aggaaaagaa gaactggcag
    21961 aattggtacc agtgagtgag aaacaaacga tcacaaactg gcttcaagaa actgaggatg
    22021 gattgaaagt gcaacgtctg cgaggcggga tccctatacc taaacttgaa aatatccgtc
    22081 cccatatgaa acggatcgag atcggagcag acttgaatgg agtggagctt gcacaagttt
    22141 ccagagtatt gtctacgaca agcgaattga atcgtttcat tgatgatctt tctgacagtg
    22201 aaatcgaatt tgcacgtttg tatatgtggg cagatcagtt agtcacgatc cctgttttga
    22261 gccgccgttt aaaggaagca atcgatgaag acggacgcgt gactgatgac gcttctcctg
    22321 agttaaagag cataagacag aacattcggc gaagtgaaca agctgttcgt gaacagttag
    22381 atgggatcgt ccgtgggaaa aatgcaaagt atttaagtga tgcgatcatc accatgcgaa
    22441 atgaccgcta cgttatccca gtcaaacaag aataccgcgg tgtatttggc ggtgtagtcc
    22501 acgatcaaag tgcttcagga cagactttat ttattgaacc aaaacaagtg gtggatttga
    22561 ataatcgttt gcgtcaatac caaatcgctg aacgaaatga gattcaacgt attttaagtg
    22621 aactttctgc tgaacttgtt ccccatcgtc aagaaatcat ccacaatgct tatgtgattg
    22681 gtaaaatgga tctgatgaat gcaaaagcac gatttggtaa agaagtaaaa gccatcgtgc
    22741 cgggaatcag tgaagacaat catgtcgtct tgaaacaagc aagacatcca ctgatcgatc
    22801 aggaaaaagt agtttctaat gacatcacaa tcggaaaaga ctatcaagcc atcgtcatta
    22861 caggacctaa cacaggtggg aaaacgatca cgttgaagac tttaggtctg ttacaactga
    22921 tggggcaagc aggattaccg attcctgcag gtgaagaaag tcaaatcggt atttttgagg
    22981 aagtatttgc ggatatagga gacgaacagt cgattgagca aagtctatcg accttttctt
    23041 ctcatatgac gaacactgta gatattttat caaaagtcaa tgaaaaaagt ctggtattat
    23101 ttgacgagct cggtgcagga acagatccgc aagaaggtgc tgctctagca atcgcaatct
    23161 tagatgatct tggtaaaaag tctgcttatg tgatggcaac aacgcattat cctgaattga
    23221 aagtttatgg atacaatcgg gcaaatacga ttaatgccag catggagttc gatgtggata
    23281 cactaagtcc aacgtatcgt ttgctgattg gcgttcctgg acgaagcaat gcgtttgaaa
    23341 tctctagccg attaggtctt gatacagaag ttattgatga agcgaagcag ttgatgaatg
    23401 atgagagcca agatttaaac gaaatgatca cagatttaga gaatcgccgg aaaatggcag
    23461 agaccgaata tctggaaatg cgtcattttg tttctgaggc acaagaactt cacgatgatt
    23521 tgaaagaagc ttacagctat ttctttgaag aacgtgaaaa agaaatggaa aaagcaaaga
    23581 aaaaagcaaa cgaagtcgtg tcagaggcgg aagaaaaagc agaaaaaatc attgctgata
    23641 ttcgtaaaat gcaacagcag attgggcaag gaaatgtgaa agagcatcag ttgatcgatg
    23701 caaagaccca attagcaaat ctacatcaag aagaaacttt gaagaaaaat aaagtattga
    23761 agaaggcgaa agaacaaaaa acgttgaaac cgggagacga agtgttggtt acgacttacg
    23821 gccaaagagg gacgctttta cgtaaaaacg ggaatcagtg gcaagtagaa atcggtatat
    23881 taaaaatgaa tgtgtccgaa gacgagctga cgcctgttgc tcctcaaaaa gagccgacac
    23941 aacgagttat ccatgctgta cgatctgaat ctagtagtca cgtgccgaat caattggacc
    24001 ttcgaggaaa acgatacgaa gaagcgctaa gtgaggtaga tcaatatttg gattctgcca
    24061 ttctagctgg ttatcctcaa gtgactattg tccacggaaa aggaacaggc gcacttcgca
    24121 agggaattac tgattatctt aaaaaccatc ggagcgtcaa aagctttgag tttgcaccgg
    24181 caaaccaagg cggaaatggt gcgacaattg ttaaatttaa gtaaagctta ctaaaggtaa
    24241 gcagatggtt cgctatccaa gtatagaaat gctatactaa tcatagattt taaaaatgga
    24301 ggtctttgat atgtcacaag taattacaga taagacattt aatgaagaaa cagataaagg
    24361 acttgtattg attgattttt gggcaacttg gtgtggtcca tgtcgtatgc aagcaccgat
    24421 tttggatcag ttggaacaag aatatgatga agaagaattc agaattgcta aaatggatgt
    24481 ggatgaaaat ccagaaactc ctcaacaatt cggtatcatg agcattccaa cattaatgtt
    24541 gaaaaaagat ggtcaagtag tagaaaaagc tgttggtgtt cattcaaaag aacaactacg
    24601 tcaaatgatc gatcaatatt tataaaaact tacagcaaaa ataagctgag ggtgggactg
    24661 aatccagttc cgtcctcggt ttttttgcgg ttgagttaag gttattcaaa ttaatatttt
    24721 ttgagtataa ttacgttgtc aggctggatt gtttttttga cagacatgct ataataactt
    24781 taattgaata tgaaggggag taccagatga acgaacgaat caaaaacaaa ttagctttac
    24841 ttcctgatca gcccggctgt tacttgatga aagataaaaa tggaacgatc atttatgtcg
    24901 gcaaagccaa aatattaaaa aatcgtgtcc gctcatattt tactggcagc cataatacga
    24961 aaacggaacg gcttgttagt gaaatcgaag actttgaata catcgttaca gaatcgaata
    25021 tcgaagcatt gctcttagag atcaatctga taaagaaaaa tgatcctaaa tacaacatta
    25081 tgttaaagga tgataaaacc tatcctttcc ttaagataac gaatgaaaaa tatccccgtt
    25141 tggtgattac tcgtaaagta ctaaaagaca aagcactgta ttttggacca tatcccgatg
    25201 tcggtgcggc gaatgagaca aagaagatcc tcgatcgatt atttcctctg agaaaatgca
    25261 aacctagtca gacgaaggag ccttgtcttt actatcattt gggacaatgt ctgtgtcctt
    25321 attatttcga cgtagatccg aaagtctata cgaaaatcgt tgatgaagtg aaaaagtttt
    25381 taaacggagg acacgaaaaa atcgagtcag agatccatca gaaaatggaa aaagctgctg
    25441 aaaacatgga gtttgaaaaa gctgcagaat accgagacca gatccgtgcc attgagacaa
    25501 tcatgactcg ccaaaaaatg acaaatactg atttactgga tcgagatgta tttggttatg
    25561 cgattgacaa aggatggatg tgtgtccaag tgtttttcgt aagacaagga aagctaattg
    25621 aacgagatgt ttcaatgttt cctttctata acgaagcaga agaagatttc ttgacttacc
    25681 tcggtcagtt ttatcaggaa aatgagcatt tcattcctaa agaagtcttg attcctgata
    25741 cgatcgacaa gcccagtgta gaagctttgt tagctacgaa agtgctgcaa ccaaaacgag
    25801 gagaaaagaa aaaattagtt gaattggcga ataagaatgc ctcagttgct ttgaatgaac
    25861 ggtttgatct gattgccaga aaacaggaac ggacgatcgg agcagttgaa cgtttaggaa
    25921 acgcgatgaa tatcccgacc ccaatacgta tcgagtcttt tgataactct aatatcatgg
    25981 ggacgaatcc tgtggctgcg atggtcgtat tcattgatgg aaaaccggca agaaaagaat
    26041 atcggaaata taaaataaaa acagtcaacg gaccggatga ttatgcatca atgagagaag
    26101 tcatctatcg cagatattca cgagtgattc gcgaagggtt gcctttacct gatttgatct
    26161 tgatcgatgg aggaaaagga caagtagatg tggcaaaaga tgtcttagaa aatcaattag
    26221 gaatcgatat tcctattgct ggcatggcaa aaaacgataa acataaaaca aatgagttgc
    26281 tattcggtat agatttggct gtagtaccgc ttgagcgaaa ttcaccagag tttttcttac
    26341 tccagcgaat ccaagatgaa gtccatcgat tcgcaatcac tttccatcgg cagctacgca
    26401 gtaaaaacag ttttgcttcc cgattagatg aaattgaagg gttaggacca aaacgaaaaa
    26461 aaatgctgct taaagagttc aaatcgttga aaaatatcac agcagccacg acagaagaat
    26521 tacaagaaat cggactgccg aaaaatgtcg cacaaaacgt atttgataaa ttacatcagg
    26581 aatgaggaaa gggcacttta caaagaaagt aaagtgtcct atcattttat gtacaacaaa
    26641 aagagcgttt acgttttgtt ggtaaataat aaatcgctta cgtctggtgt ctaaggatat
    26701 atgtgatttc ttgaagaagg agtgaagaaa tgttagaagt caaggatctt gttaaaactt
    26761 ttgggaacta cacagctgtc gatcatgtct ctttccaaat ccccgatgga aaaatcatgg
    26821 ggctaattgg ccaaaacggg gctgggaaga caacgacttt tcgtttgatc ttggattttt
    26881 tgacacaaga ccaaggagaa gtgttatgga atggccatca tcttggggag aaagattatg
    26941 atgtcatcgg ttatcttccg gaggaacggg gactttatcc gaaagtaacg attcaagacc
    27001 aactgcttta ttttgcagaa cttagaggga aatcaagaaa agagattgaa ccaaaaatcg
    27061 atttttggat ggaaaaattt caggtaaaag ggaaaaagac ggataaagta aaatctttgt
    27121 ctaaaggaaa tcagcaaaaa gtccagctga tcgccacgtt aatccatgaa cctaaattga
    27181 tcattttgga tgagccattc agcgggttag atccggtaaa tgctgaattg ctaaaagatg
    27241 gaatattgga attgaaaaaa catggttcat gtgtcatttt ttctagccac aatatggata
    27301 atgtagaaaa aatctgcgat catttgatca tgttgcgaaa cggaaaaatg gttttgaatg
    27361 gaaaagtaca tgagattcgt gaatcatttg gccgtacgaa gttattcttg gaatctggtt
    27421 taagccaaca ggaagtagaa gaaatcgaag gcgtgaaaaa agtgatcctt catgaagaca
    27481 atacgttaga gattacctta gaagatcctg aagctgggaa agaaatcttt actcgtgcga
    27541 ctcagttcgg ctacattcct atgtttaacc agcaaccgcc aactttggaa gagattttca
    27601 aattgaaagc aggtgaaatc gatgcgtaag ttttggatta ttaccaaaga tgtctattta
    27661 aaaaatgtta agtcgatttc tttcttgatc atgatattag tcccttttat catgctgggt
    27721 atcatttatg tagcaggaaa ctttgctcaa caaaatagtg atgtcaataa aatcggcgta
    27781 ttggctgata atgagcaaat cagtcagcaa atgagccaag tcaaaacaga tgattttgag
    27841 ttcaaagcag ttgattctga agaaaaagca caaaaagagc tttcggaaga aaaaatcgat
    27901 gcgtatttag tagtctcaac tgagaatgat aagataaatg ggatgcttta tagcgaaaat
    27961 tcattaggac aaagtacaca actactgata caacaacaac tgactggctt gcaatcgatg
    28021 cttcgagcaa gtcaattagg catttcacca gaagaagtag ctagcttgag ccaacaagca
    28081 agtttgactc gacaaaaagt caattttgat tcaaaaggaa aaatgacagt tggagaagat
    28141 aactcgacag tccaatatgt cgtaagttat gtagcaacga ttgtcttgtt cattattatt
    28201 ttgacgtatg cgcagatcat tgcacaagag atcgcttctg aaaaaggcac gcggatcatg
    28261 gaagtgatcc tgtccagtac gactgcacaa aagcattttt acggaaaact aacaggtgtt
    28321 ttacttgttg cattaacgca aatggcatta tacggtatta tttttggtat aggatacagt
    28381 caattcaaag atatggaaat ggtcaaaaat atattagacg gtatctcatt ggataacttg
    28441 attggaccat tcttgtggta ttctcttcta ttcatgatct ttggcatttt gattttttct
    28501 gttttagcag ctttatgtgg ctctttggtc aacaaagcag aagatacagc aaaagcaatt
    28561 ttgccagtga cttatctatc tctaggcgga tatatgcttg gattgatact aggagcttca
    28621 gatccaaaca atatcgttat tcggatcact tcgtatattc cattcttatc ttcatatatt
    28681 atgccggttc gcttagcaaa tgagacagtc ggtatgggtg gagcattgat ttccttgttg
    28741 gtacttgtag ccgtaacatt cttattgatg atcttgtctg caaacatgta caaatcgaat
    28801 gtactcgtgt atagcgaagg cggattatgg agttcattga aacaatcgat ttcaattatg
    28861 agaaatgaac gaaaaaaagc ataaaatata gaaaaatggc tctttgtcaa atgatgttgg
    28921 taaagagaaa tcttagaata attggagatc aagtggatca gttcctcttg atctttttta
    28981 gttgaaaatc aatgcgttgc catgacgaat catgatgcgc aatttgaaat tataaaagct
    29041 acgaaagcca tatgcatttc gttttaacac tttgatatgg ttgttcagac attcaagtgg
    29101 gccatttgaa taactttgtt gtaaagcatt tttgatttgt ggcagaaatt tctggaacgt
    29161 tctcaaggtc gtctgatagc cttcgggaag catgatatta ttggtaagta gttcttcaaa
    29221 cgatacgtag tctcgttcct ttacagcgta aagaaagtct tgatataact gatagcctac
    29281 acgcaaagca gggctccctt tcagtaagcg atccacaata tcggtctctg ttaaatgagc
    29341 tttgaagcca ggtcgccaaa ggcgtcgagt ataatcaagc ttgcgttcat ctttttgaag
    29401 aagtttccag tatcttttta attgcttacc taattgatag tgtttttgtt ctttgctttt
    29461 taatagttgg ttcgtttctt tgattcgatg attgcgaaag gtacgaccaa tatgttggac
    29521 gatgtgaaac cggtcaatga tcagctgtgc ttttggaaac agctttttaa cgagcgatac
    29581 ataaggagcg tacatatcaa tcacgatcca ttcgaccgct tcacgaattt ctcgtgaaaa
    29641 atgactaaaa taacgtttta aaaaaggcag ttgtcggttc tcgacaatgt ccaacaagcg
    29701 ctgtgtttgt ccatccatca tgatgaaact catagcacct gaaacacccc gaactgattt
    29761 gaattcatca aaacaaagta ccttaggtaa cggtgtccga aaaggattca acggacgata
    29821 aaatcgcttc ataatacggt aaacagaaga gaccgataga tttttctgag cggcgatctc
    29881 tgtcattgag gtattttttt tcagacgagt cacgacagac cagcggactt tctcactgat
    29941 gaaacagtgt tttttcgcaa cagaagtatc tgcgacaaag gtccgctgac aagctttgca
    30001 tttgaagcgt tgctttttca attcgaggta ggtacggcat tcagaaacat cgttcattaa
    30061 caaccggacg gttgttgtac cccaacgcac aatatctgaa gcaatctggt gtttacagtg
    30121 gatgcattct tgggctgtat aaatcaagtt tccacgataa atatgacaga tttgcccttt
    30181 gtatttttct tttgtgagac aattttcgtt aaaagtaact gataagtcta gaatatctaa
    30241 tgtttctttg ataaggtgag tgtaagacat cttgattcct ccttttgggt tggttcgcca
    30301 aattaattct aaaggatttc aaggtgtctt ttctatatgt tttggaaaat aaaaaagatg
    30361 ctggtgtcat ccaccaacat caaaaagtat agagctagaa aaatatagaa ggaagctgga
    30421 catttattgt tcccagcttc ttttttgtat agacctattt ctattttttt attatattgg
    30481 atatatatat tcattgaaaa tagagcataa caactttata atgttgagta taccaatttt
    30541 tggaggcgtt ttaatggacc gctatttaga tttactttca gagaagttcc ctacaacgga
    30601 agatgtcatc acggaaatca tcaatctcga agcaattttg cagcttccaa aaggaacaga
    30661 gttatttttg agcgatatac atggcgaatt tcctgctttt gatcatattc tgcgtatcgg
    30721 ttcaggaaat cttaaagaaa agatcaaaga ccttttctct gaacaaatga cggaaacgga
    30781 gatcagccaa ttcactattt ttacggctta tccagaatat gtgttgacga ctgattggta
    30841 caaaaaacaa gataaatccc agctgatcca tcgtttgatc gatcttttac ggtttacgac
    30901 agtcaaatac actcgatcaa aagttcgaaa aggattgccg aaagaatata gctatatcat
    30961 tgaggaactc ttgtatatcg atgatcgtat caacgggaaa aaagactatg taaaaaaaat
    31021 cattgaacaa cttgtcttga tgaaggaaga agaaaaattt ttaacaaaac ttgcgcagac
    31081 gatccaaaaa tctgtcatcg atcatttgca tatcgtgggt gatatttttg atcgaggaac
    31141 tcaagcagcc aaagtaatgg atcgaatcat ggatttttcc tctgtagata tccagtgggg
    31201 gaatcatgat attttgtgga ttggtgcgta ttgcggctct gaggcatgtt tattgacgct
    31261 tttacggatt gccgcacgct acaactattt atttgaatta gagaaagaat atggattaaa
    31321 tttacgtcca ttattttctt ttgcccatca aacctatcag aagaatccag tatttacccc
    31381 taaaaacagg aaagatctat ctgaaagaga gcaagaagaa ttagaaaaga tccatcaagc
    31441 actttctatc ctccaattca aatcagaaca tcagttgttg aatcgacgac ctgaatttaa
    31501 aatggacgat cgtaaattgt tggaaaaaat caactatgag gaatcaacga tcgttttaga
    31561 aggtcagctc tatgagttga aagggacttg ttttcaaacg atccagccag atgatcctaa
    31621 aaaattcttg gaagaggaac aaaaagtgat cgatagtttg atgtattcat tccagcactc
    31681 gttaagaatg caaaaacaca tgacgttttt gatcgaaaaa gggggaatgt atctcacgaa
    31741 caatcagcat gtactgttcc atggctgtat acctgtagat gaaaaggggc agctcttagc
    31801 atttgaatta gaccaatctt atcgcggaaa agaactgctg ggattttttg aaaaacagat
    31861 aactgaaagc gcaaaagatt tgactgcaaa tgaagatttg gcgaccgatt tgatttggta
    31921 tgcgtggagc gggccatttt cgcctttatt cggcaagagt aaaatggcaa cttttgagcg
    31981 ctattttctc acagagcctc atacgcacgt cgaaaaaagc aatccctatt atcacttgcg
    32041 agatgaagag tggttcagtg aaaaaatttt ggcagaattc gatgcaaaag aagaaggttc
    32101 agcaatcatc aatggacaca cgccagtaaa agtaaaaaaa ggagagtcac cgatcaaagc
    32161 aaacggaaaa gcatttgtta ttgacggagg tctatccaaa gcttatcaaa aaacaaccgg
    32221 gattgctggc tactcattac tatgcaattc ttacggtttc caaatcgtga cacattatcc
    32281 gttcttgcct attgaacaac aatttgcaaa aaaatctgat caaacgaatg taaaaaaagt
    32341 gatcgagcag cagctcccac gcaaacttaa tcggaatacg acaaaaggaa tggaattaca
    32401 gcaacagatt actcaactcc aaaggctgtt ggattatcga aaggacgatt gagaaaaatg
    32461 ctagccaaca ttgctcaata gactaaaaag cgactgcaac aaaaggcgtg tcgcagtctc
    32521 cttcggaatt agctcaaaaa acggtaaaat attagaagct atttttctgt ttatttctct
    32581 aatactcagg tcagggcgct gatttcacga cctcttcctt aaacggtgtt ctcctgccaa
    32641 ctcctcggtc acaagtcaca atatccaaca aacatgagaa gctgtttttt gaaattgctc
    32701 cttgtgactc agagttgaac ggcaggctca cgacctaatc agaaaaacga ttaaaattca
    32761 ttcacaaaga aatcgaataa acgctgttca gatgaatcta cacgatgaga aagttccgga
    32821 tgccattgga caccaaggat tttgtgcata ttatctttac tttggactgc ttcaatgagt
    32881 ccatcgttgg aaaaggcgat ggcttttaac aaaggagaaa gatctttgac tgcctgatga
    32941 tgataggaat tcacaaagta tgaatcaggg agaaggtcac ttaataaact gtccgaaacg
    33001 attttgactt cgtgagttgc aaactgcggg gcagtgggct gttgttcgtg ttttacggac
    33061 cattttggat ataatgaaag atcttgataa agtgttccct ccaacacgac attcaatagt
    33121 tgaagtcctc ggcagactgc aaaaatcggt tttctttgtt tcaatgcttc caagatcaat
    33181 gcctgttcga attggtctcg ttgaatattt gtttccgtca gttttggatg agggtcttcc
    33241 atgtaaagtt gcggactaac atcctgaccg cctgcaagaa tgagtttgtc gattttctca
    33301 attagttgag gcgctgaatc tggagacatg actggcagaa taagaggtaa tccttgagca
    33361 tctaaaactg catccacaaa tccctgcggt gtatagctta cttgatttcc ttggaaagta
    33421 tctgtcgctc gaatcagttg attcccggca atgccgatga ttggtttcat ttttattccc
    33481 cctaaagtag actaattgta acaaggattt aaaaaaattt aaactatgta agacttgccc
    33541 ctatacttat ctgttcttgc tgtgttacta tctgtaaaga gaattttcgt gtgaaattca
    33601 tgacgatagt tgaaaatatt gcttaatttg ttataataaa aagtaagcag aaataaaaag
    33661 gttgggcgta agcttcaagg atctagttct tcaaggggtg agaaggacat tttattgaag
    33721 gggcctgcct atactaagtt atctttagag gtacatactt tcaatgaaaa aagaaagcat
    33781 gtacctcttt tttaaatcga aaggaagtga agcaagtgtt agaagctttg gaacaaatcc
    33841 gaaaagcaga agaagaaaat gagaagcgtg ctcgaaccct acggcagcag ttgaagacct
    33901 atgcagaaga aaaagagcaa gaattggaga taatccaaac gaacagtcgg caacagctga
    33961 aacagttggt agaagaaaaa gaacaattcg aaaagcaaga attagaaaaa gaaaaaatga
    34021 aattgaaagc agaagcagac aaggtgcaag caactttgaa agaccaatat caaaagcatc
    34081 agagtcaagc aatcggtgca atcattgaaa gggtgaaaga aacttatggc cgtcactaag
    34141 atggaaaaag tgaccctcat ttcagataaa agaaatcagg agaggatttt acaagcagta
    34201 caaggaatcc aaaatgttga aatccgggat ctgtatcaaa atagaacaaa caaccagtgg
    34261 gtagaaaaat attttccaga taccacgatt attgataaag aagcaaaatt aagcgcttta
    34321 ggcaatcggc tgaaagacat ccaagaagct atccaattca tacaacacca cggcgatagc
    34381 cgtcaaaaga aactccattt gaaacgtcaa gaactttcct tacaagactt ggaagcaaat
    34441 tattccgaag aaaatttcat gaataaatta gaagaagtcc ttgatttaaa agaaaagtgg
    34501 gaacagctaa ctgaaaaacg tgatcgttta gaagaagagg aagaatggct tcttcattgg
    34561 caacagctgg atattgctcc aagtatgtat gcaagtaaga cgaccatttt tgagatgggg
    34621 actgtgagtg cagcaaattt tgaaagtttc aaggaagaac taagtaagct tgatgacgtt
    34681 tatatggaag aaatagatta tacgccaaaa caagtttatc tttcgtatgt tgctttaaaa
    34741 aaatcagttg ctgctataga agaaattgct gggaaatacg ggttcaataa agaaacgtat
    34801 ccttatgaac aaaatcctaa agaaaaattg caagaaacca aacagcaatt atccattgtt
    34861 cacgagcagc aaaaaaagat aacgacagcg ttaggtcagt gcagcggcta tatccaagaa
    34921 tttgaatggg tagaagaagt cacacttgcc gttgcagaac gggaaaaagt aaaagaacgg
    34981 tttatccatg ccacttatct gatcgttttg caaggttggg tagatgcaga agaaaaacag
    35041 aatttgatgc atgtgttaaa cgaaactgta tcagaagaag atatatacgt tagtttcgaa
    35101 acaccaaact cagaagagat tcagcaggaa gtccctacta aattgaaaaa ccatccgctt
    35161 atcgaacctt ttgaattgct gactgaaatg tacagtctgc caaaatatga agaagtagat
    35221 ccaacgcctt ggatgacacc attttatctt gtcttcttcg ggatgatggt cgcagatgtt
    35281 ggttacggat tgctgatgct cattgggaca atactagctc aaaaactatt tgttttaccc
    35341 cgcgggatga aacgtttcgt aaaattcttt gagattctct cggtaccatc gatcatctgg
    35401 ggattgatct acagttcatt tttcggacag gcattaccaa aagaactatt tggtatccgc
    35461 ttgcctttcc caatcctatc tacgacggat gacgtcaata cgattttgat tttatccgtg
    35521 attttcggtc tgatacagat cttggttgga ttattcgtgg cagcaaagga aaatatcagg
    35581 aggaaagatt acttgagtgc tgtcagtgat ggttttgcct ggcaagggat cctgattggg
    35641 atcgttattg cattgattgg tgtcatgttg ataaaaaata cagcatttgt ttatcttggc
    35701 gcggggctat cgatcattgc tgctttatgt atcttaatcg tgccgattat ccaatcgccg
    35761 tctaaagtca agggtgctgc caaaggagcg tataatcttt acggattgac tggttatatc
    35821 ggtgatttag tcagttatac acgtttgatg gcactaggta tttcaggcgg aagtattggc
    35881 gcggcattca atatgctagt tgctttcatg ccgccagccg ctcgattcag tgtagggatc
    35941 cttttgatca tcgctttaca cgcactgaat atgtttttga cactgttaag tgcctatgtc
    36001 catggtgcac gattgcagta tgtcgaattt tttggaaaat tttatacagg aggcggacgt
    36061 gcctttgacc cgctgaaaac ggcggaaaaa tacgtcaaca tcaatcataa gaaaaaaaac
    36121 aataaataat tggaggattt ttatacaatg atggattact taattagtca aaatggtgga
    36181 atggtatttg cagtattagc gatggcaaca gcgacgattt tttcagggat tggttcagct
    36241 aaaggtgttg gaatgacagg tgaggcagca gcagcgttaa caacaagtca acccgaaaaa
    36301 ttcggacagg ctttgattct gcaattgctt ccaggtacgc aagggttgta tggattcgtt
    36361 atcgcctttt tgatttttat caacttagga aatgatatgt cagttgtaca aggattgaac
    36421 tttctaggcg cttctctgcc gattgctttt acaggtttgt tttcaggtat cgcccaaggg
    36481 aaagttgcag cagcaggtat ccaaatttta gcgaaaaaac ctgaacatgc tacaaaagga
    36541 atcatttttg ctgcgatggt tgaaacatat gcgattttag gtttcgttat ttccttccta
    36601 ttagtattga atgcctaagc ataaaggagg aaaagcagat gaacgccatt gaaaaaatca
    36661 tctctcagat gaatgaagca gcagagcaag aaagagcggc gttagagcaa gaagagcgaa
    36721 tgaagatcga tcaaaacttt gaacaaaaac gtacgcaaat cgaaacagaa catcaaaaac
    36781 aaaaagaaaa acaaatcgaa ttacttgaaa aaaaataccg tcagcttagg aatcgccaac
    36841 aagtagaagt acgacaagaa aatttgaatg cgaagcagga atttttacga cgattgtttg
    36901 cagatgcaat agctgaaatg gagaattggg atgaatctga acaaatccag tttatcaaaa
    36961 attcattgta ttcattacct ttgacaggca aagtaacatt cattgctgga gaaaaatcag
    37021 cagcttatct ttctcagaca ttgctggatg aatggaacaa tgaacttccc tttatgatgg
    37081 ttttaagtga tgaaactgtt gctgatcaag cgggttttct gataaacgat caaggagttc
    37141 agtataattt tcttttcagc agccttgtac aagatatcca aggaacgatg agttttgaga
    37201 ttgccaatca attattcgaa taaggaggga tcagtgtatg gaataccacg aactaaatcc
    37261 cttgattcgc ggaagagagc ttgaactttt gtccaatgag acatttgaac ggatgattca
    37321 aacaaattct gtcgaagcac ttggtgaaat attgaaatcc acgatttaca gtccatatat
    37381 ttatgacgga tatgaatatg acttcgaaca gaatctttta aaggagcaga gccgtctatt
    37441 tcgctggtta aaggaatcag caccggagcc tgaaatcgtt tggatctata cgatgcgcta
    37501 tactttccat aatctgaagg ttttgacaaa agccgagatg acaaatcaaa atctggacca
    37561 tttgtatatc gacgatggat tttattcatt agaaaccttg aaggatgcca ttcatacgca
    37621 agtttcaaat gaactccctg aaagcattat ggacagtata cgagaagtac gtgattattt
    37681 tgaagagtct accattcttc agggaattga cgtgatttat gatcgacagt ttttgacaga
    37741 gcagcgacgg cttgccgata agctaggata tcaagaattg ctgacagaag tcattgcttt
    37801 tattgacttg accaatatca caacgatggc tcgaggaata ttgcagaaaa gaacatatgg
    37861 gttcatgacg acggtacttt ccagctcggg aagtatcgaa aaagaagtat ttttgaactt
    37921 tgtagataaa gatatggaag attacacgaa ttttctatta gagacagatt atgctgattt
    37981 tctgcgccct gtcatccgtg agggaaatct tgatctcgta aagcttgaac agttgaaaga
    38041 tgattatcta agttcattgt atcaagaagc acaaacccag gctttcggac cactgccact
    38101 actagccttt ttgaacgcaa aagaggtaga aagcaaaaac cttcgtttac tggttatcgg
    38161 caaaagaagc catttcgaac cagaaacaat cagaaagcgg gtgagacaag tctatgacgc
    38221 ataagatcgg tgtagttggc gacaaagatt ctgttttgcc attcaaattg tttggctttg
    38281 atgttcgtta tggtacaacc aagcaaaccg ttcgtcaggc aattgaagaa atggcaaaga
    38341 atgagtatgg cgtgatttat gtcacagagc aatgtgcagc attgagccca gaaacgatcg
    38401 aacgttacaa ggaacaaatg atacctgcta tcgtattgat tcccaatcat caaggatcat
    38461 tagggatagg gctggaagaa attcaaaaaa acgtagaaaa agcagtaggt caaaatatct
    38521 tatagtatga aaaggagata gaaattttgc aaattggaaa aatcataaaa gtctccggac
    38581 ctcttgtaat ggctgagaac atgtctgatg ccagtatcca agacatgtgt ctagtgggag
    38641 atctaggagt tatcggagaa attatcgaga tgcgtggcga tgtggcgtct attcaagtat
    38701 atgaagagac ttctggaatt ggtccaggcg aaccggttcg ctctactggt gaagctttat
    38761 ctgttgaatt aggaccaggg atcatttcac aaatgtttga cggaatccaa cggccattag
    38821 atacctttat ggaaatcaca caaagtaatt tcttagggcg cggagttcaa ttgcccgcat
    38881 tggaccatga gaaaaaatgg tggttcgaac caacagtaga agccggagaa acggtgagtg
    38941 ctggagatgt tatcgggatc gtagaagaaa caaaagttat aaaacataaa atcatggtgc
    39001 caaatgggat aaaaggaacg atcaaaacaa tcgaaaaagg ttcattcact attgatgaaa
    39061 cagtatgtgt cattgaaacc gaaaatggtg acaaaaattt gacgatgctt caaaaatggc
    39121 cagtaagacg tagtcggcca atcaaagaaa aattaaaccc ggatgcaccc atgatcactg
    39181 gacagcgagt gattgataca ttcttcccag tgacaaaagg aggagcagca gctgtacctg
    39241 gtccttttgg tgcagggaaa acagtcgttc agcatcaaat tgcaaaatgg gccgatgtgg
    39301 acatggttgt ctatgtggga tgtggcgagc gtggaaatga gatgacggat gttttaaatg
    39361 aattccctga attgattgac cctaatacag gagaatcgtt gatggaacgt acggtattga
    39421 ttgctaatac atcaaacatg ccagtagcag ctcgagaagc ttctatctat acgggaatca
    39481 caattgcgga atatttccgt gacatgggtt atgacgttgc tatcatggca gactccactt
    39541 cacgatgggc agaagcactt cgtgagatga gcggacgttt ggaagaaatg cctggtgatg
    39601 aaggttatcc agcttatctt ggttcacggc ttgcggaata ctatgaacga tctggaagag
    39661 tcattgcatt aggatcagaa ggtcgcgaag gaagtataac cgcaatcagt gctgtatccc
    39721 cgtctggagg ggatatttcg gaaccagtca cacaaaatac attgcgggtc gtaaaagtat
    39781 tctggggtct tgattccagt ttagcccaaa aaagacattt tccttcgatc gattggatac
    39841 aaagctattc tctttattca acagaagtag ggaagtatat ggatcatatc cttcaagctg
    39901 actgggcatc catggtcacc gagggcatgc gtattttgca ggaagaacag cagctgaatg
    39961 aaatcgtgcg tttggtaggg attgattccc tttcagataa tgaccgtctg acattagaag
    40021 tggcaaaatc cattcgtgaa gattatttgc agcaaaatgc ttttgatgat gtcgatacat
    40081 tcacttcaag agagaaacaa ttcaaaatgt tgaagatgat cttgaccttt ggtgaagaag
    40141 gacgccgtgc gttgtcgtta ggtgcttatt taagtgagat catggacggt accgtatcga
    40201 ttcgtgaaag gatcggacga agcaaatata tcccggaaac agaattagaa aaactagatc
    40261 aaattattgg tgaaatcaag caaacgatgc aaacgatcgt ttcagaagga ggcatgagca
    40321 atgattaaag aatatcgtac gatcggtgaa gtcgtcggtc cattgatggc ggttgaaaaa
    40381 gttgctggtg tcaaatatga agaattaatc gaagtccgta tgcaaaatgg cgagattcgt
    40441 cgtggacaag tattggaagt ccaagaagat aaagcaatgg tccaaatatt cgaaggaaca
    40501 agtggtatca atttacgtga ctcttctgta cgatttttag gccatccttt agaactaggt
    40561 gtatcggaag acatgatcgg tcgagtattc gatggtttgg gacgaccgaa agataacgga
    40621 ccagagatcc tgcctgaaaa gcatttggat attaacggag aagtaatcaa tccagtagct
    40681 cgggattatc cagatgagtt tatccaaaca ggtatctctg caatcgatca tttgaataca
    40741 ttagttcgtg ggcaaaaatt accagtattt tctggctctg gtcttcctca taaagaatta
    40801 gctgctcaga tcgcgcgaca agcaacggta ctcgattcat cagatgattt cgccgtagtc
    40861 tttgctgcta ttggaatcac ttttgaagaa gctgaatttt tcatggaaga ttttcgtcaa
    40921 acgggcgcaa tcgaccgttc tgtcatgttt atgaatttag caaacgaccc tgcaatcgaa
    40981 cgtatcgcta ctcctcgtat ggctttgact gctgcagaat atcttgctta tgaaaaggga
    41041 atgcatgttt tggttatcat gacagacatg accaactatg cagaggcatt gcgagagatt
    41101 tctgcagcac ggcgagaagt tccaggaaga agaggatatc ctgggtatct ttacacgaac
    41161 ttagctactt tattcgaacg agcaggaaga atccgcggac taaaaggatc ggttactcaa
    41221 attccgattc taacaatgcc agaagacgat aaaactcatc caatccctga cttgactggt
    41281 tatattacag aaggacagat catcttgact agagagcttt acaagagtgg gattcagcca
    41341 ccaattgatg tactcccgtc tctttcgcgg ttaaaagata agggaactgg acccggaaag
    41401 acaagagaag accatgcagc tacgatgaat cagctttttg cagcctatgc acaaggaaaa
    41461 caggcaaaag aattagccgt agttttaggt gaatctgctt tatctgatgt agacaagatc
    41521 tatgcgaagt ttgctgagcg gtttgaaaaa gaatatgtca atcaaggatt ctacacgaac
    41581 cgttcgatta ctgaaacact tgatctaggg tgggaactgc tatcgatgtt gccaagaacc
    41641 gaattaaaac gaatcaaaga cgacatgctg gacaaatatc tgccagaagg gaagtgagtt
    41701 ctgatggcac gattgaatgt gaatccgacc cgcatggaat tgactcgatt gaaaaaacag
    41761 ttgacaactg ccacaagagg gcataaatta ttaaaagata agcaagatga actgatgcgt
    41821 caattcatcc tgttgattcg aaagaacaac cagctgcgtc aagaaatgga agagtctatg
    41881 acagaagcaa tgtctgattt tgtattggca aatgcttcga tcacggaacc tttcatcgaa
    41941 gaattatttg ttttgccggc ggaaaacgtt gatttgtcta tcgtagacaa aaatatcatg
    42001 agtgtaaaag tacctgtgat gaattttgaa tatgatgaac agctgacaga cacacctttg
    42061 gaatacgggt atttgaattc cagtgccgaa ctggatcggt caatcgatcg gtttactacc
    42121 ctgcttccaa aacttttgga actgacagaa atcgaaaaaa cttgtcaact gatggcgagt
    42181 gaaatcgaaa agacacgtcg gcgagtcaat gcattagaat atatgacgat ccctcaatta
    42241 gaagaaacga tctattatat tcgtatgaag ctggaagaaa acgagcgcgc agaagtaaca
    42301 aggttgatca aagtaaaaaa tatgggtacc actgactaag tggtatcttt ttttaaaaga
    42361 ggaaaacgat gattaaacaa agcaaacaaa taaagaaacg tttgccggct gttcagcttc
    42421 tcgcattagg attttttctc ctgattttag ttggcggcgg cctacttacc ctaccatttt
    42481 ttagtaatag tgggcatggg acaaatttta tcgatgcgct tttcactgca acttctgctg
    42541 tctgtgtaac gggattgaca acattgaata cagcagagca ttggaacagt gctggtcaat
    42601 ttttgatcat gcttttgatt gagatcggcg gtcttggttt tatgatggtt ccgattctat
    42661 gctttgcatt gataaagaag aaagtcagtc tcagcacacg tattatatta aaagaagcgc
    42721 tgaatctaga agaagtatca ggtgtgatga agctcatgct ttacatccta aaattggcct
    42781 ttgttatcca attgattggt acggttgctt tatcttttgt atttgtgcct gaatttggct
    42841 ggttgaaagg tatctggtac agtttgttcc atgcaatctc tagtttttgt aatgcagggt
    42901 ttgatttgtt aggagacagt ttagccaatc accagcaaaa tgtttatttg atcatggtgg
    42961 tatctgcttt gatcatttct ggtgggttag gcttcatcgt atggcgtgat ttactggaat
    43021 atcatcagaa aaagaagatg acgattcatt caaaaatcgc attaaccgtt acaggtaccc
    43081 ttttattagg cggattcgtt gtgttctttt tcacagagcg caatggggca acgctagtgg
    43141 aagggaatat gtttcaacga ttggcaaaca ccttttttat gagtgtgaca cctagaacgg
    43201 ctggatatta ttcagttgat tacctccata tgagccatgc aggtctgatt ttaacgatgt
    43261 ttttgatgta catcggcgga acatctgggt cgacggccgg cggattgaaa acaactacgt
    43321 taggtgtgtt gctgatccag atgcgttcga tgttgaaagg acgtacgaga gcggaagcat
    43381 ttggccgtac catccgacaa ggcgcagtgt tgcgggcact tactttattt tttgttacgt
    43441 tgacgttgtg tgtagtcgcc atcatgattt tatcggtgac tgaaacgata cctaaaactt
    43501 ctggcatcga atatattgct tttgaggtct tctctgcgtt tggtacagta ggtcttacga
    43561 tgggattgac gccagacttg actgcaatag ggaaattgat cattatagcg cttatgtaca
    43621 ttggacgagt tggtgtattg actgtggtct tctctctgat gacaaaagca caaagaacac
    43681 aatcaaaata caaatatcca gaagaaagtg tcatgattgg ataatttcat cttctgacag
    43741 aaacctagtg atttagtaaa ataagaaaat aaaataattt ttcttgcttc ttggatactt
    43801 taagtgcaaa atgttcgttt attttttgag gcactacccg ttgttgaacc tcgggtagtg
    43861 cctctttttt attgaaagga atcaataaga tttgattcct ttatttatca ataggttata
    43921 attcatattt ttttattttt tcttttacga tacttagtcc agaagctaaa atttcaaggt
    43981 tattagagga tagggaaatt cgaacgaatg catctggttg cgcacgtgta gtaaagcgtc
    44041 tggaatgata aagtcggata ccgttagcaa gaaagtcatt ctcaattatc ttagggtcaa
    44101 tctcaggatc aactggaaga attcgatagt aagggacacg attcgttggt ttttctactt
    44161 tgaaaagaga atcaaaaagc tgattggctc tttttgtaag acttaattta gtttctaaga
    44221 tcctatttgc ttcatttgaa tggaggattt gagtgatgac ttccacatct aatccagaag
    44281 ttttcacatt tatattaaaa attgcttgtt ctatcttgct ttggacatgt actggaacta
    44341 caagataagc aactcgcaat ccagaacaaa taaattttgt cattccagct aagtagattg
    44401 tttggttagg taataaattt tggaaaggag gaatgatcgt atgttgatag taagttgtta
    44461 aaaatgaatg gatatcatct tcaataaccc atagaccttc tttttcaatc acttcggcta
    44521 attgctctcg tcttttgtga gagatttgaa accctatagg attattacaa gaaggcataa
    44581 gaaagacacc atgaatcttc ttttttctac attcctgaat caatatatca ggtcgcattc
    44641 cttgctcgtc aaaatcaatt ggaacgatct ctaaatgata taactgagcc aattcaatga
    44701 aattagcgta tgtatatcgg tctgttgcta ttctatcacc tggagagaaa agtgcagaaa
    44761 gtgtgatagc caatccattt tgaacaccgg aaacaataga tagagattct aaggacggaa
    44821 tgactccttg agtttttaac caatttgctg cagtagtcaa ttgaaaattt gtgcctttgg
    44881 gatcttggta attaagcaaa ccaacaagat ttgggttatt actagttgat tgaataaacg
    44941 ggacaacgaa tttattacat tcttcaaaag aactaaccag accaaaatca atgatatgtt
    45001 cggttacttc attggtagag atggtgatgg actcaaagac attgggagaa ataaatgtgc
    45061 cacttccaat attcgtatac agcaaacctt tctcaattcc atatttataa gccttgccaa
    45121 cggtagtaaa atttaaatct aaataatcag ctagctctct ttgggagggt aactgagtgt
    45181 ttttctgtaa tttattcttt aaaatatctt gttctaattg ttgaatcaac gatcgataat
    45241 aaggacgctt caatttttgt ttgtctggtc gccaagttaa cgggtaattg tcataagaat
    45301 taataggcat tttctccact ccttaaaatt gtataccata caattttacc attgattgta
    45361 ttgattttag aagttaatct tttgtgagga gggaattgtc tatgaaaatt attaattttg
    45421 aggaagtagt agcttctttt agttttgaag aagcgattca agtaatgaaa aaatgttttt
    45481 ttgattatga aagtggtaaa atcagccaaa atcctcgatt ggtggaaatt ttacctgacg
    45541 gaaaaggcaa aaatatgttt gcgatgatgc ctgcttattt gggggagaac agatattttg
    45601 gcacaaaagt cattaccgct tttccggata atcatcagta tcatttacct tctcatttag
    45661 gagagatttt cttgtttgat gctcaaaacg gactacccaa agcattgata aacgcaaatg
    45721 cagtcacctg gattcgaaca gctgctgtat ctgcgctggc aacagattac ttagcaaatc
    45781 cgcatgcaga aacacttgct ttaattggag ctggtcaaca agcattgtcg catttgactg
    45841 cgatttctac cattcgtccc atcactacag tgtttgttta cgatatttta gaagaaagac
    45901 gaaatacttt tatcgctaaa gctaaaaaac agtatcctaa aataacgttt ataaatgcaa
    45961 tatcagtaga agatgcagta cgtccagcgg aaatcatttg tacgttgacc tctagtaaag
    46021 aggcatttct tcaaaaagag tgggtaaggg aagacgcaca tatcaatgct gtgggcacat
    46081 ttactcctga tacaagagag ataagtacat cgttgatgga aacttctcaa atctatgtag
    46141 atgattactc agctgcctta acagaaagcg gggacctgct tatagccatg gcagaaggag
    46201 atttagagga atcttgtatt gttggtacat taggcgaatt agtttctgag aaaaaaacat
    46261 tttataaagg agaatcgagg ccgacgattt tcgacgcagt tggtctagct attgaagatc
    46321 tttgttgtgc agaatatatt tataacaaat tagagaaagg ggctttaaaa tgaacttaga
    46381 ttattacgta gttgatgcat ttgcagatga ggtgttcaaa ggaaatccag cagcggttta
    46441 tgtgctagaa gaatggctgc cagaaggtac gatgcaaaaa attgccattg aaaacaattt
    46501 atctgaaaca gcatttacag tgaagaaaaa ccaagaattt gagcttagat ggtttacgcc
    46561 agatcgagaa atcgatcttt gtggtcacgc gacattagca acagcatttg ttttatttaa
    46621 ctattacaaa atacctgatg aaacgattaa attttctact caaagcggta atctatttgt
    46681 gacaagaatt aaagattatt attacatgga ttttcctagt attatgccga aaaaagtgcc
    46741 gatattgtct gaatatgaag aagcgattgg ggctaagatt aaagaagcct acttagcacg
    46801 tgatttattt ttcgtattag aagatgaaaa aacggtggct aaattgcagc cagattttac
    46861 agctatcaaa aattttgaat taggaatagg agtcatcgtg acagcagaaa gtgtgcaaaa
    46921 agactttgtt tctagaacct tctttccaaa attaacaatc aatgaagatc cagtatgtgg
    46981 atcagcacat tctaatctta ttccttattg ggcagaaaga atgaataaaa caaaattgtc
    47041 agcttatcaa atgtcatcaa ggggcgggga tctagaatgt gaattaaaag aagatcgtgt
    47101 gataatagga ggaaaagcag ttttgttttc taaaggtacc tcgtttattt gatttctttc
    47161 aaatgaatta aaaataaatt ggtggagagt ggcaacctta tagctacaat ggttgttaaa
    47221 aaaagacgca aactttttca atttaatgaa aagtttgcgt ctcattttaa ttattggtgt
    47281 ttgtttttta ttcaatcaag agtaaaatgc cccatactaa ctatcctcgg atgacttcga
    47341 ttttataacc atcagggtca atgacaaagt agtaagaagg cgcagtgcca ggtaatcctt
    47401 ttaagtcagt aatctcaaga ccggcagctt tatgttgctc gtgtaatttt tctagatcat
    47461 cagctgctat agcgatatga ccatagccgt ctcctagatc atacgcacca tgatcatagt
    47521 tgtaagtcaa ctctaattca tagtcatccc caggaagtgt caaataaaca agtgtaaatt
    47581 tgttttctgg aaaatcacgg cggcggcttt cttcaaaacc aaatgccttt tgataaaatt
    47641 cgattgatgc ttcaagatct ttcactcgaa cacatgtgtg agccattttc atagattcat
    47701 ctctcctttt ccttttactc aatcataaca aaaagaagta aggaaatagt tgatttgtgc
    47761 ttgttcatga gaaatttctc atgtaaaatg gatagaaaat aaaactgtta aggtggaaac
    47821 tattcatgga taaaccaatt tttggtgaaa aaaagcaatt tgctgattat caaacccgtt
    47881 atgcagcata tattgtcata gaaagagatc agcagattgc agtaatcgag gctccaaatg
    47941 gtgctttttt tcttcctggc ggggaaattg aaggagaaga gacgaaagaa gaagcgattg
    48001 tccgtgagct gatagaagaa atgggaattg tggcagaaat ttcttcttat ctgggagaag
    48061 ctgatgagta tttttactcg aattatcgtg caacttatta ttataatcca ggatatttct
    48121 ttgttgctga ttcttggaag cgtataggag aaccaactga aaagaccaat aaaatttggt
    48181 gggtgagccc tcaagaagcg cttgtgaaat tgaaacgtgg tagccaccga tgggcagtcg
    48241 agagatggat cgaaaaaaat aaaaactgaa agaaagcgag tagatcagct ttctctcagt
    48301 ttttttattt attacagtac atattttttg attgcttcag ctacaccgtg ctcgtcgttt
    48361 gatgaagtga tgacatttgc tgcttctttg acaagagaaa cggcatttcc cattgctact
    48421 cctagaccag catattcaat catggataag tcgttctcgt tatctccgat agccatgatt
    48481 tcatcttgat caataccaag gtgctgtgcg agattcgcaa ctgccgcacc tttacttgct
    48541 tctttattta gtacttcgaa gtaatagggc gcacttttga ccgttgtgta tttatcacgg
    48601 aaagtttgcg gtaaacgcgc aattgctgca tctaaaattt ctggttcgtc gatcatcatc
    48661 attttgacga tgttcatgtc tggtgtcatt tcttcaggtg ttcgatattt tagcggcatc
    48721 ttgactaaac ttgcttcgtg gatcgtataa gcgctgatat ctcgattagg cgtgtagatc
    48781 gctttgtcat cgatcgaatg gagatgactg ccgacacggc gtgccatcac ttcgatctct
    48841 aggaaatctt catgtgtcag tccgtgacgg gcgatgattt tacctgattt cgtttgttgt
    48901 acgagtgccc cgttataagt aataacgtaa tcattatctt gaaaaaggtt caactcgttt
    48961 aactgttcct ggacacctgg taagggacgg ccagtgcata aaacgatcga aacacctttt
    49021 gcaacagcag cggcaatcgc ttcttttact tcttttgtta cttctctttt ctcatttaat
    49081 aaagtaccat cgatgtcgat agcgactagt ttgatagaca taatttcctc caatatattt
    49141 taatttattt caatcaatgc gccattacgg atatggctgg caaattcttg ataagtttcg
    49201 tcaaataaat cataatgatc acgcaaggac ggatcaacca tttcttttgg aaagaaaaat
    49261 cgctcatctc cttgttcttg tcccgctaat gcagcaacaa gctgactaac ctgtgataat
    49321 tctatcaaag ttccgtcatt tcgcaaaatc tcgatttgtg tgcgatgtct gccttgttct
    49381 gggcgataaa aatcataagg tagatcgtag ctagaattca cagcagtgta ataaacagga
    49441 ttatatccga ccttttctac taacaaagtg agttcttgga ctaattttga atcccggttg
    49501 ccagaaaaag tagcagattt cagtggttta cgattcaaaa aacgtttagc taaatcattt
    49561 aaaataggat cagcagattc agaccattga gtaaagtaag tgcccaagac cccatcatca
    49621 agtttgaggt attcttctaa agtaaagctt ccgtcaagaa atggcagaag taattgagaa
    49681 tggagttcaa aactgctggc atcttcttga tacaactcgt gtgcgcgatg aagcagatgc
    49741 tccaaaatta cttccatacc acgggaaaca ggatggaagt acacttgcac atacatttga
    49801 tatcggctaa cgatataatc ttccactgca tgcatgccac tcatggaaaa agcgatcccg
    49861 ccttcataag gacgaatcac acgtaatatt cgggtaagat caaatgttcc atattctgtt
    49921 ccagtgaaat aagcatcccg aagcaaatag tccattcgat ctgcatcgat ctgactagaa
    49981 atcatttgga caacttgtgg gttggggtaa gttttttgga tcacactagc taccttttca
    50041 ggaaaacctt cttctactcg gttaagaatc tgatacactt ctgtttctgg agaagtgatg
    50101 atctctacag tgatggcttc atgattcgta tggaagatat gttcaaatgt atgagagtaa
    50161 ggaccatgac cgacatcatg caacagtgcc gcacataaag tcaccagccg ttcctgatca
    50221 tcccagcctc cgttgcctat tttttctttt gaaaaatttc gagaaaagat atcgcagatt
    50281 cgtcttgaaa tctcataaac gcctaatgaa tgagtaaaac ggctatgttc tgctccgtga
    50341 aaagtgaaag aagaagtgcc taattgttta atgcgtcgta gacgttggac ttctttcgaa
    50401 ttgatcaaat ctaagattac ttgatgctgg acatggatat agttatggac tggatcacga
    50461 aaaacttttt ccataggcag catttgatat ttataagggg ttgtcataat gtagctcctt
    50521 tatgttatta cgttgttcca ttctctggat ttggttgttc cattgctcca gctggatttc
    50581 tttttttagc cagtcggtag aatcctgtaa atcacttgca gaatacagct tttcaaaagc
    50641 ttttgcaaat gcttctttga cttcttttac aaaaaggggt tggtcaagca gttcttcaag
    50701 tgttgccatt gtagcaggat ccactgcagg atagccattt gtaccaaagt cttcttttaa
    50761 agcagattga taaaatcgct tcatgacttg cccgcgtttt tcctgattac cgttgacact
    50821 taagtacatc ataacagaaa tcccattttt gatccgacgc tgagcgatac cggcaaattt
    50881 tttgccatgg atgcttaaat cataagttcc aggacaataa gaatgggtga tttcgaatgc
    50941 ttgtatcggt gcctttggaa aagcgtattt agtcaatagg agcatttgct cgtaagcgtc
    51001 atctatggtt attttttttg tgtcactttg ggggaaaatc aaagaaatat tcaaaattcc
    51061 ttcatctgat acgatcccta atccacctga atttctgatc actggagcgt aacctgcatc
    51121 tttcagtgtt tggatcccgt cacttagaaa aggagttcga acatccttca tacctaagat
    51181 gactgtgcag tcatattgcc agaaatgtaa aaagatttgc tggtgttctt tagaatattc
    51241 cgttaatata tctgttaatg caaaagggga aaagtcgttt ttttgataaa tgtgctgatc
    51301 tagtcgaatc gcagggttca ttcaaccggc tcctttgtat aaattcatat tttctacaat
    51361 attatactct aaaatcaaag agttgttttg gaaaaaaggt tttctgtaaa aatggacaag
    51421 ttggttgaca aaacaaaaga aactaggaac aatgtagcta tgagaagtaa aggagaatga
    51481 ataatggaat tatcacaagg aacaccgatt tcaatcaaat tgcgtacaaa agtaaaacag
    51541 caaggggaag tgcaagattt ttattttgat ttgaaagggc aaatggtgac aattggcgat
    51601 acgctgtaca tacgttataa agagattcag gaggagacgg gcgatgagat tcctgtaacg
    51661 atcaaattag ttccagatgg tcatgtacag ctgatccgtg caggagaaat gcggatgcgt
    51721 ttgaaatttg gctataaaga acggttagaa acatcctatc gaacaccata cggtatgatc
    51781 cagattgcta cttttacgaa agaactgcat gttagtttga aagaccgtcc cacaggaggg
    51841 aaggttcgaa tcgattatga tctgttcatg ggaccggaaa aaatcggcga atattatttg
    51901 accttggatt ttacagcgta aatataaaag aaaagtttga ttttttgtgt ctgattttgt
    51961 atgataagga gtagactgtg aaaggacgtg tcctgattgg aaattagtgt atttgaaggt
    52021 gcaaacaaaa gcgaattatc aatgatcgaa gtagcacacg cgatcttaga acaacgtggc
    52081 gatgtcatgg atttttctga tttagctaac cagatccaaa actatcttgg gaaatcagac
    52141 agtgaaattc gtgattcact cgcacaattt tatactgact tgaatattga cggcagcttt
    52201 atctcattag gggataatcg ttggggcttg cgttcatggt atgctatcga ttcgatcgat
    52261 gaagaagtaa accatggaat ggacgaagag gatgaagata ctccacgtcg ccgtaaacgc
    52321 aagaaagtca acgcctttat caatgatgat gaagacgcga tcgattacaa tgacgatgac
    52381 ccagaagata cagatctgac agaagaagat gacgatgatc tgtttgatga cgatgatgat
    52441 gaagatgaag aaatcgctgc ttataattct gacttgcaag aaatcggagc agatgacccg
    52501 acagatgatg aagaagactt gccaggaaat atcgaagaag atttaagtat catagacgaa
    52561 gacgatgatg atgaagatta tgaagaggaa gaattttctg actctgaaaa agaataaaca
    52621 ttattttctt acgagaggtt gtgacagaga cgatcagcct atcagaataa ggaggtgtga
    52681 cacgattttt gtcacacctt cttttttgta tataaaaatc ccaagtaaat acataaaaaa
    52741 taaaaaaatt gaaactttta gcgcaccttg tacgtcatat taagtgaggc agaaaggagt
    52801 ggagaatgat ttttactgga tataagaaaa aacggcagat cgaaaaatac cgtcacctgc
    52861 tgaatggact catcgaaact gattatcaaa aaatgttccg tatcacgatg aattacgtac
    52921 atgataaaga agaggcctta gatgtgatgc aagatagttt tcataaagcc ctgaactatt
    52981 ttgaaaaaga aaaagagatc gagcatttta gtgcatggtt ttatcggata ttgatccgca
    53041 cggcattaga tagttggcgc agacaaaaaa ggacgcctgt ggacttattt gaaattcaag
    53101 atatccccca acagttacct ttagaaactt ccgttgcaga gctccatatg attttagata
    53161 aaatagacag tccggaaaaa gagattctga tcctctactt ttttgaagga tttcgtttga
    53221 aagaaattgc gactatcttg gatatgaatg aaaacacagt taaaacgaaa atgtaccgct
    53281 cattgaatgc tttacgtcaa atcctagaat gaaggagaga agagtgccat gtattcccaa
    53341 aaagaaaatt taaaaaaact aaagaaaact tataaagaac aagcaattcc caaagggctt
    53401 aaagaggaga tccgccagcg ttttattatc gaagagcaat catttataaa agcaaagcgg
    53461 agaaagagac gtctccaagg gttcggagta tctttggcta tcgtaatgat tacaacaagt
    53521 agccttcttt ttaatagtca agttcgaagc tttgcagaag atttacccat cttaggatca
    53581 gtcattgaat tgatcttagg cgaacgcttt actgatcgat ctgaaaaaat cgatattcag
    53641 gtaccgaaga tcaatacaca aaatgagaaa gaaaataaaa caattcatgg attaaatcaa
    53701 aaatactttc gcgaaggaca agcagatttt gaaaaagctg aaaaagaata tggaaatttt
    53761 gaaacggatc attaccaagt tttgggagat tatcagaaaa tcgtagacga taaccggttt
    53821 ctcgtgattg aaaggcagat cacccaaaca gcagcagata gccatgttga aaaaagatac
    53881 gacacaatcg acaagaaaaa cagtgtccag ctctcacttc cacttttatt caaagatgac
    53941 acctatctat ctgttttgac aaaagaagtc aaacggcaga tggccgaaca agtcaaggaa
    54001 gatccatcta agtactattg gactgagcaa gatattcaag aagggacgat agaaaaacca
    54061 acacttgtta cgcctacacg aagtttttat ttgaacaaag agcatcaatt agttttgact
    54121 ttttctcaat acgaaattgc tcctggttat atgggtacac cagaatttgt cattcctaag
    54181 tccgtgacaa aaacgatttt ggcatcggaa gattatttgg atcattagac gagaagtagt
    54241 tgcgactagc agtttcaatt ttcaagtaag tacaagtaaa taataagaag agaaaaagag
    54301 aaagaagaga tatagtgaaa agaaattatt tagttatcag tattattgct ttatccgtgc
    54361 ttttgttttt cgaaattact tcggtggcct ccttgatcac cgaagtaaaa ggacaaacac
    54421 actcttcgga gcattcgtca caatctttca aacaatcaga acaatcaacc attaaaagcc
    54481 aatcaactga aacagctaac gtcaaagaag ccattcaaga atcagcggaa aaggtacaaa
    54541 gtcagtcatc acaatcaaca caagacagta caaaaacgag ccgagatccg gctgtcaaaa
    54601 gcgtcgcaat cagctttgat gatggaccag gagcgaccac aacaccgcag cttctgcgaa
    54661 tcttaaaaga aaaaaacgtg catgccactt ttttcgtctt aggagaaaat acggcgcagc
    54721 atcctgaaat cgtgaagcag acagctgaag cagggcacga gattggcaat tacacatacg
    54781 atcatcagaa tctggccact ctttctgccc agtcgatgac ggaggaagtg acgaaagcag
    54841 acactgagat caaaaaagtc actggcaaaa cccccacttt tgttcgtccg ccctatggtt
    54901 ctgtcacgaa tgtaggagca acgatcatcc agcgaccgat catcgaatgg tctgttgatt
    54961 cagaagattg gaagacaaga aatcctgatt tgattttgca gaagatccaa gcaacagtat
    55021 acgatggggc aattatttta ttccatgata tctatcctga aacgattcgt gccgttcctc
    55081 aagtgatcga ttatctgaaa gagcaagggt atcgcatcac gacagttggg gatttattag
    55141 ggcatccaac ggctgtcgag aactattatg gcagaaacga tcatcggcca gttcagtaag
    55201 tgagacaagt atctttaata aataacacat tttaataagt agttgaacac ataactacct
    55261 tacttgcaaa ggcttaacaa tatctataca ggtacaacaa aaaactgtcc accaattttc
    55321 tttggcggac agttttcact taaaaataaa gaagcaacta ttcgtaaatt atgctaaaat
    55381 agcagtagaa cgcgtcctga gggaatcgaa cccccgtctc aagaaccgga atcttacgtg
    55441 atatccacta cactaaggac gcaagcactg ggataattgt aactaaacag ataaaaaatt
    55501 acaaggggat tggacaaaaa aatgaaattt gagcaaaatt tttcacaaaa acagcagcag
    55561 acacagaaac tagccatgac gcaacagttg caacagtcca tccaagtttt acaatataac
    55621 agtgaggagc tgttggcatt cgttgagaat caagcaatgg aaaatccatt ggtggaagtt
    55681 gttgagccgg aatggcagcc agattacata aaagcttctt catcttctta tgaaggagaa
    55741 gagacaaatt acctaaatca gattcctgat acaaaagggt ctttatttga ttcattgatc
    55801 gaacaagtcc atttgaatta ccgagatact tttttgagga aaatcgttct ttatcttgta
    55861 gaatacatcg atttgaacgg atttttgacg attagtttag aagaagccgg aaaagagatt
    55921 ggagctacgc ctatccaaat gctcgatgcg ttgactctga ttcagcaatt agaaccagct
    55981 ggagtaggtg cacgtagttt gcaagaatgt ctcatgcttc aaacagaacg agatgattat
    56041 gcgccggaat tagcttatat cgtgttggaa gaatgttttg aggaactggt ggagcgaaaa
    56101 tggaaagaaa tcgcacaacg tttttctgtt gatctacatg ctgttcagca gatattcgac
    56161 tatttacaaa cgttgagtcc aagccctggc cggattttcg accgtagcag cgaattgtac
    56221 attattcctg atgtccgggt attagtggac gacgataaaa atgtacaagt tatctcaaac
    56281 cgaaaaaatc agccaaatat acgctttcaa gagagttact tcaaacaaat gtcacaacaa
    56341 gcagataaag aaactgaaaa ttatttaaaa gaacggaaac aggaattcga atggttgaag
    56401 aaaacgatcc tacaacgtgg ggatacaatt ttgcgggttg ctcaagtcat tgtttcccgg
    56461 caaaaagctt tctttatcga caaggaacgt ccaatcaagc ctcttacgtt aaaggaagtt
    56521 gcaactgaga tagatgtgca tgaatccacg gtcagtcgtg cagtcaacgg gaaatatttg
    56581 gaaacagatt tcggtgtatt tgaattaaaa aaatttttca ctacacgaat tcctacgaat
    56641 agtacagaac agacagaaga cctttcagca gatacagcga agaaaaagct gcaggaatta
    56701 gtcgatcaag aagacaaaaa caagccgttg tcggaccaga aattggttga attgctgaaa
    56761 aaagatgaga tagcgatctc aagacgaaca gtagcaaaat atcgtgattt attaggaatc
    56821 ccgagttcat caaaaagaaa acgttatgat aactaatcag tcaaaagact agaaaaagta
    56881 tttcactttt tctagtcttt ttagttgaaa aacgagagcg aaactgttag acttacagtc
    56941 gtgaggttga gatttccctt tattgatgtg aaattcgaat agttaatttc tttttttatc
    57001 gtatatgggt cgttttaagt ctacattgga cgtaaagtgt ccaactaagg agatcgctat
    57061 gctggatgaa ataaaaatga ttgaatctgt tgcaccagat attatcgaag tgttgcaaga
    57121 acgctataaa attttacgaa atatctattg gatgcagccg attgggcgtc gaagtttatc
    57181 ggaaagtatg gggatcacag aacgagtctt acgaacagaa acagaccttt tgaaaaatct
    57241 gaatttgatc gacacctcaa aaagcggtat gacgctgaca actaagggcg aagaagttta
    57301 tcaaagcctt gaaaacttca tggatcagct tttaggtacg catcaaaccg aacaacagtt
    57361 ggcagaatat tttggtaacc aacgctgtat cgttgtttca ggaaacagcg aagagcagac
    57421 caaagtggct gatgcattcg gcgaagcgct gagcgaagcc ttagaccgac tacttccaga
    57481 aggcgagaat atcatcgctg tgatgggagg aactactatg gctgtggtag ccgaacaatt
    57541 gtctaatctg gaaaataaga aacggcataa cctgtttgtc cctgcaagag gcgggattgg
    57601 tgaagcgata actgtacagg caaattctgt cagtgcaaga atggctgcta aagcaaacgg
    57661 taaccaccgc gcactttatg ttccggaaca attaagtctg gcaacttaca attctttact
    57721 caatgagcca tctatccaag aagtcttaaa cttgattagt gaagcgaatt gtgttattca
    57781 tagtattggg cgtgcattac acatggcagc acgtcgtaag atgactgaaa aagaattagt
    57841 catgctgaaa caagcaaatg ctgttgcgga atcttttggc tactttttca atgaaaaagg
    57901 tgaagtcgtt tataaggtcc ctagaatcgg catagaattg caagatttgg aaaaagtccc
    57961 gatcatcatg gcaatcgcgg gcggaaaatc aaaagctaaa gcaattcgag cttatatgaa
    58021 aaatgcacca aaacaaacgt ggctcatcac tgatgaagct gctgcaaatg agattttaaa
    58081 aggggtaacc ctttaaaata aatatttttt tgattttcat aaggaggaaa tcttaaatga
    58141 cagttaaagt aggtattaat ggttttggac gtattggacg tctagcattc cgtcgtatcc
    58201 aagatgtaga tggaatcgaa gtagtagcaa tcaacgactt gacagatgct aaaatgttgg
    58261 cacacttgtt aaaatacgac acaactcaag gacgtttcaa cggaacagtt gaagttcatg
    58321 aaggttcatt caacgtaaac ggaaaagaag ttaaagtatt agctaaccgc aacccagaag
    58381 aattaccatg gggcgaacta ggcgtagata tcgttctaga atgtactggt ttcttcactt
    58441 ctaaatcagc agctgaaaaa catttaacag ctggtgctaa acgcgttgtt atctctgctc
    58501 ctggcggaaa cgatgttcca acaatcgttt ataacacaaa ccacgaaaca ttaactggta
    58561 aagaaacagt tatctcaggt gcttcatgta ctactaactg tttagctcct atggctaaaa
    58621 cattgaacga caaatttggt gttgttgaag gattaatgac aactatccac gcttacacag
    58681 gtgaccaaat gactctagac ggacctcatc ctaaaggtga cttccgccgt gcacgcgctg
    58741 ctgctgcaaa catcgttcct aactcaacag gtgctgctaa agctatcggt ttagtaattc
    58801 ccgaattgaa cggtaaatta gacggagctg ctcaacgtgt tcctgtacca acaggttcat
    58861 taacagaatt agtaacagtt cttgaaaaag aagtaactgt tgacgaaatc aatgcagcaa
    58921 tgaaagaagc ttcaaacgaa tcttacggat acaacacaga cgaaatcgtt tcttcagata
    58981 tcgttggtat gacttacggt tcattatttg atgctacaca aactaaagta atgacagttg
    59041 gcgacaaaca attagttaaa actgttgctt ggtacgacaa cgaaatgtca tacactgcac
    59101 aattagtacg tactttagaa tacttcgcta acttataaga tagacaaacg tacaagtgta
    59161 aattatagac atgtacattt aaaagcgggg aagcaatcgc gcttcttccg ctttttttga
    59221 taataagttt attcaggagg aaataaaatg gctaagaaaa cagtgaaaga tatcgattta
    59281 aaagacaaaa aagttcttgt ccgtgttgac ttcaacgttc ctttgaaaga cggcgtgatc
    59341 actgacgaca ctcgtatcaa agcagcattg ccaacaatca actacgttct agaacaaggc
    59401 ggaaaagcaa tccttttctc tcaccttgga cgtgtaaaaa cagaagaaga taaagaagga
    59461 aaatctttag ctccagttgc aaaacgtcta ggcgaattat taggtaaaga agtaacattc
    59521 gtacctgaaa ctcgcggaga acaattagaa gaagcaatcc gcaacatgaa tgacggcgac
    59581 gttgtcgtat tcgaaaatac acgatttgaa gatattgacg gtaaaaaaga aagcaaaaat
    59641 gatacagaac taggtaaata ctgggcttca ttaggcgatg tatttgttaa cgatgctttc
    59701 ggtacagctc accgtgcaca tgcttctaac gtaggtatcg cttcaactgg tatcccaaca
    59761 gttgctggat tcttgatgga aaaagaaatc aagttcatcg gtgaagcagt agaagaacca
    59821 aaacgaccaa tgattgcaat ccttggtggc gcaaaagttt ctgataaaat cggcgtaatc
    59881 gaaaacttga ttcctaaagc agataaaatc ttgatcggtg gcggaatgac ttacacattc
    59941 tacgaagcaa aaggaatcaa gatcggtaac tctctagtag aagctgataa agtggaacta
    60001 gcaaaagaat taatcgaaaa agcaggcgac aaacttgtct tgccaatcga caatgtctgt
    60061 gcaccagaat tttcaaatga tgttgaaaca caagtaatcg aaggagacat tcctgatggt
    60121 ttgatggctt tagacatcgg cccagcatca gtaaaattat ttgctgatac attacaaggt
    60181 gcaaaaacag ttgtttggaa cggacctatg ggtgtatttg aaatgagtaa ctttgctaaa
    60241 ggtacaatcg gtgtttgtga agcaatcgct aacttagaag gcgcaacaac aatcatcggt
    60301 ggcggagact cagccgcagc agcagaacaa ttaggatttg ctgacaaatt cacacacatc
    60361 tcaacaggtg gcggcgcaag cttggaattg cttgaaggta aagaattacc aggactagca
    60421 gcaatcaacg ataaataaac gaccaagttt gctcaattct gacgagtaat cgaacaaaag
    60481 gtgaagggag ttttcatgac tccctagcca tttttcaata aattaaggag tgtgttcctc
    60541 aatgcgtaaa cctattatcg caggtaactg gaaaatgaac aaaactgcat cagaagcaaa
    60601 agaatttgct gaagcagtaa aaaacaaaat cccttcaaat gacaaagttg attcagtcat
    60661 cggatctcct gcattattct tacaagaatt aatgacatca gctgaaggta cagaactaaa
    60721 aatctcagca caaaactgct actgggaaaa ctcaggtgcg ttcactggtg aaacttcacc
    60781 agcagctctt gctgatctag gtgttcatta tgtgatcatc ggacattcag aacgtcgtga
    60841 atatttccat gaaacagacg aagacatcaa taaaaaagcg aaagctatct ttgcaaatgg
    60901 tatgacacca atcctatgct gcggcgaatc tttagaaacc tatgaagcag gcaaaactgc
    60961 tgaatggatc gaaggacaaa ttacagctgg tttagctgat ctttcagctg aacaggtgtc
    61021 aaacatggtt attgcttatg aaccaatctg ggcaatcggt actggtaaat cagctgacgc
    61081 acaaatcgct gacgaaattt gtggagttgt acgtcaaaca gttgaaaaac tttatggaaa
    61141 agaagtatca gaagcagttc gtatccaata cggcggttct gtaaaaccag aaaatattgc
    61201 tgaatacatg gcaaaagaaa atgttgacgg tgctttagtc ggtggcgcaa gcttgcaagc
    61261 agattcattc ttgtcattgt tagacgctgt aaaataattg ttgaaaattt gcgaaaaatg
    61321 caagaaatgt ttgcactaat gagcaaaatt aactaaaatc gtatatgagg gaaatccctt
    61381 aggaaaaaac aaactcaaag gagagacaaa acatgtcaat tattactgat gtttacgctc
    61441 gcgagatctt agactcacgt ggtaacccaa caatcgaagt agaagtatac acagaaagcg
    61501 gagcttttgg ccgcggaatg gttccttcag gtgcttcaac tggtgaatac gaagcagttg
    61561 aattacgtga cggagacaaa gctcgttatg gcggtaaagg tgtaactaaa gctgtcgaca
    61621 acgtaaacaa catcattgct gaagcaatca ttggctacga tgttcgtgac caaatggcta
    61681 tcgacaaagc tatgatcgct ttagacggaa ctcctaacaa aggtaaatta ggtgcgaacg
    61741 ctattttagg tgtttctatc gctgttgcac gtgcagctgc tgactaccta gaagtacctt
    61801 tataccacta cctaggcgga ttcaacacaa aagtattgcc aacaccaatg atgaacatca
    61861 tcaacggcgg atcacatgct gataactcaa tcgacttcca agaattcatg atcatgcctg
    61921 ttggcgctcc aacattcaaa gaagctctac gtatgggtgc tgaagtattc cacgcattag
    61981 cttcaatctt gaaagctcgc ggattagcaa cttctgttgg tgacgaaggt ggatttgctc
    62041 caaaccttgg ttcaaacgaa gaaggttttg aagtaatcat cgaagcaatc gaaaaagctg
    62101 gctatgtacc tggtaaagac gttgttcttg ctatggatgc tgcttcttca gaattctacg
    62161 acaaagaaaa aggtgtttac gtactagctg actcaggcga aggcgaaaaa acaactgacg
    62221 aaatgatcaa attctacgaa gaattagttt ctaaatatcc aatcatctca atcgaagatg
    62281 gattagacga aaacgactgg gacggattca agaaattaac tgacgtatta ggcgacaaag
    62341 ttcaattagt tggtgacgac ttgttcgtaa caaacactca aaaattatct gaaggtatcg
    62401 aaaaaggaat tgctaactca atccttatca aagtgaacca aatcggtaca ttaacagaaa
    62461 ctttcgaagc tatcgaaatg gctaaagaag ctggctacac agcagttgta tctcaccgtt
    62521 ctggtgaaac agaagattca acaatctctg atatcgctgt tgcaacaaac gctggccaaa
    62581 tcaaaactgg ttctctatca cgtactgacc gtattgctaa atacaaccaa ttactacgta
    62641 tcgaagacca acttggtgaa gttgcagaat acaaaggttt gaaatctttc tacaacttaa
    62701 aaaacaaata attcttttct aatgaagata catgatcgac tattgaaaga tagtcgagta
    62761 tgatcactta gttaagtaat cgaactctct acttgctgaa atatcagcag gtagagagtt
    62821 tttttgcaac tagcttctgt atatgatgtt tacaaagaat ggtacaagaa aagatcatcc
    62881 ttgaaaaagg aaagctaaca ataactagca tcatataaga gcttttaagg atagaacagt
    62941 agaataggaa tctatgggca agtcctctcg tataaataaa ataggaggga agtagcagca
    63001 tatttttcat aaagtggctc tctttcttca tttttacttt ggttgctcct atttgaaaat
    63061 tcgagcacac tcaaatacaa taaaaagaaa tggcaaggga gaggattaga tgaaaaaaga
    63121 agaaaaaatc gcgattttac aagagattat ccgaattaaa tcagtgaatg gaaacgaagg
    63181 tgaagtagca gcctatttga ataagctttt ggcaaagcat gacataacag gagagattgt
    63241 ttcttacaga gatggtagag ataatttgat tgctcgttac caaaagggcc aatcaggaaa
    63301 agtgttaggc ctatctggac atatggatgt cgtggcggct ggtgatgaat cgtcttggac
    63361 ctatgcaccc tttgcagcag aaatccatgg gaaccgtttg tacggacgtg gtgctacaga
    63421 tatgaagagt ggcttggcag caatggtgat tgccatgatc gaactgaaag agtcaggaaa
    63481 accatttaac ggaacagtca aattattagc cactgtcgga gaagaagtgg gtgaactagg
    63541 tggggaacag ctcacgaaag caggctatgt agatgactta gatgccttga tcatcggaga
    63601 gccaaccaat tacagtttga tgtatacgca catgggatca atcaactata cagtcacctc
    63661 gcacggaaaa gaagcgcata gctctatgcc agaccaaggt tataatgcaa tcaatcacct
    63721 aaatgaattc attacgaaag caaatgctga aatgaatcac ctcgctgaaa caatcgagaa
    63781 tcctgtccta ggaaaaacaa tccataatgt tacattgatc agtggcggga atcaagtcaa
    63841 cagcatccca agccatgcgc agctccaagg gaacattcga tcgattccag aatacccaaa
    63901 tgataaaatc attgcattgc tgcaatcaat cgtcaatgaa ttgaatcaag agacagacta
    63961 tcatttggaa ttgatgatcg attacaacaa aatacctgtc aaagctgatc cagattctcc
    64021 tttgattcat tgcattcagc aacaattcag ccagccatta cctttagttg gagcggcagc
    64081 aacaacggat gcagccgaat ttacgaaagc taaccattcc ttcgactttg tcgtctttgg
    64141 gcctggtgtc gttactttgc ctcatcaagt agatgagtat gttgaaatcg ataattattt
    64201 ggatatgatc gaaaaatatc aaggaattat tttatcctac ttagcgtaag atttttttaa
    64261 ta
//
LOCUS       DACDXE010000006        61688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.006, whole genome shotgun sequence.
ACCESSION   DACDXE010000006 DACDXE010000000
VERSION     DACDXE010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.006"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..2405)
                     /gene="esp"
                     /locus_tag="IWM23_000366"
     CDS             complement(<1..2405)
                     /gene="esp"
                     /locus_tag="IWM23_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAP9883040.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPE
                     DKLP"
     gene            complement(3022..3456)
                     /locus_tag="IWM23_000367"
     CDS             complement(3022..3456)
                     /locus_tag="IWM23_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883041.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3473..4678)
                     /locus_tag="IWM23_000368"
     CDS             complement(3473..4678)
                     /locus_tag="IWM23_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAP9883042.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5372..5758
                     /locus_tag="IWM23_000369"
     CDS             5372..5758
                     /locus_tag="IWM23_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9883043.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5852..6043
                     /locus_tag="IWM23_000370"
     CDS             5852..6043
                     /locus_tag="IWM23_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883044.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6557..6889
                     /locus_tag="IWM23_000371"
     CDS             6557..6889
                     /locus_tag="IWM23_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9883045.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7185..7340
                     /locus_tag="IWM23_000372"
     CDS             7185..7340
                     /locus_tag="IWM23_000372"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAP9883046.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7616..8956
                     /locus_tag="IWM23_000373"
                     /pseudo
     CDS             7616..8956
                     /locus_tag="IWM23_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9197..10813)
                     /locus_tag="IWM23_000374"
     CDS             complement(9197..10813)
                     /locus_tag="IWM23_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9883047.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10931..11149
                     /locus_tag="IWM23_000375"
     CDS             10931..11149
                     /locus_tag="IWM23_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883048.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11344..11805
                     /locus_tag="IWM23_000376"
     CDS             11344..11805
                     /locus_tag="IWM23_000376"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9883049.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11998..12237)
                     /locus_tag="IWM23_000377"
     CDS             complement(11998..12237)
                     /locus_tag="IWM23_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883050.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12261..13784)
                     /locus_tag="IWM23_000378"
     CDS             complement(12261..13784)
                     /locus_tag="IWM23_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9883051.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13802..13924)
                     /locus_tag="IWM23_000379"
     CDS             complement(13802..13924)
                     /locus_tag="IWM23_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9883052.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13954..14937)
                     /locus_tag="IWM23_000380"
     CDS             complement(13954..14937)
                     /locus_tag="IWM23_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9883053.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14961..15110)
                     /gene="rpmG"
                     /locus_tag="IWM23_000381"
     CDS             complement(14961..15110)
                     /gene="rpmG"
                     /locus_tag="IWM23_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9883054.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15131..15508)
                     /locus_tag="IWM23_000382"
     CDS             complement(15131..15508)
                     /locus_tag="IWM23_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883055.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15540..15719)
                     /gene="rpmF"
                     /locus_tag="IWM23_000383"
     CDS             complement(15540..15719)
                     /gene="rpmF"
                     /locus_tag="IWM23_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9883056.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15734..16003)
                     /gene="rpsN"
                     /locus_tag="IWM23_000384"
     CDS             complement(15734..16003)
                     /gene="rpsN"
                     /locus_tag="IWM23_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9883057.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16526..18298)
                     /locus_tag="IWM23_000385"
     CDS             complement(16526..18298)
                     /locus_tag="IWM23_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883058.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINM
                     VPAFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     VQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18252..20006)
                     /locus_tag="IWM23_000386"
     CDS             complement(18252..20006)
                     /locus_tag="IWM23_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883059.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20116..20685)
                     /locus_tag="IWM23_000387"
     CDS             complement(20116..20685)
                     /locus_tag="IWM23_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAP9883060.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20703..22127)
                     /locus_tag="IWM23_000388"
     CDS             complement(20703..22127)
                     /locus_tag="IWM23_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9883061.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(22129..22797)
                     /locus_tag="IWM23_000389"
     CDS             complement(22129..22797)
                     /locus_tag="IWM23_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9883062.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22927..23511)
                     /locus_tag="IWM23_000390"
     CDS             complement(22927..23511)
                     /locus_tag="IWM23_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9883063.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23842..24075)
                     /locus_tag="IWM23_000391"
     CDS             complement(23842..24075)
                     /locus_tag="IWM23_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAP9883064.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24090..25040)
                     /locus_tag="IWM23_000392"
     CDS             complement(24090..25040)
                     /locus_tag="IWM23_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9883065.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(25037..25879)
                     /locus_tag="IWM23_000393"
     CDS             complement(25037..25879)
                     /locus_tag="IWM23_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9883066.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25881..26600)
                     /locus_tag="IWM23_000394"
     CDS             complement(25881..26600)
                     /locus_tag="IWM23_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9883067.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27292..27450
                     /locus_tag="IWM23_000395"
     CDS             27292..27450
                     /locus_tag="IWM23_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883068.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27522..28748
                     /locus_tag="IWM23_000396"
     CDS             27522..28748
                     /locus_tag="IWM23_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9883069.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28836..29228)
                     /gene="rpsI"
                     /locus_tag="IWM23_000397"
     CDS             complement(28836..29228)
                     /gene="rpsI"
                     /locus_tag="IWM23_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9883070.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29244..29687)
                     /gene="rplM"
                     /locus_tag="IWM23_000398"
     CDS             complement(29244..29687)
                     /gene="rplM"
                     /locus_tag="IWM23_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAP9883071.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30206..30673)
                     /locus_tag="IWM23_000399"
     CDS             complement(30206..30673)
                     /locus_tag="IWM23_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAP9883072.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30793..32331)
                     /locus_tag="IWM23_000400"
     CDS             complement(30793..32331)
                     /locus_tag="IWM23_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAP9883073.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32411..33097
                     /locus_tag="IWM23_000401"
     CDS             32411..33097
                     /locus_tag="IWM23_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAP9883074.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33298..33825)
                     /gene="msrA"
                     /locus_tag="IWM23_000402"
     CDS             complement(33298..33825)
                     /gene="msrA"
                     /locus_tag="IWM23_000402"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9883075.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33909..34586)
                     /gene="pgmB"
                     /locus_tag="IWM23_000403"
     CDS             complement(33909..34586)
                     /gene="pgmB"
                     /locus_tag="IWM23_000403"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9883076.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34583..35713)
                     /locus_tag="IWM23_000404"
     CDS             complement(34583..35713)
                     /locus_tag="IWM23_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9883077.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35713..37896)
                     /locus_tag="IWM23_000405"
     CDS             complement(35713..37896)
                     /locus_tag="IWM23_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9883078.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37893..38918)
                     /locus_tag="IWM23_000406"
     CDS             complement(37893..38918)
                     /locus_tag="IWM23_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9883079.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38963..39790)
                     /locus_tag="IWM23_000407"
     CDS             complement(38963..39790)
                     /locus_tag="IWM23_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9883080.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39787..40854)
                     /locus_tag="IWM23_000408"
     CDS             complement(39787..40854)
                     /locus_tag="IWM23_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAP9883081.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40880..41716)
                     /locus_tag="IWM23_000409"
     CDS             complement(40880..41716)
                     /locus_tag="IWM23_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9883082.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41713..42582)
                     /locus_tag="IWM23_000410"
     CDS             complement(41713..42582)
                     /locus_tag="IWM23_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883083.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42652..43935)
                     /locus_tag="IWM23_000411"
     CDS             complement(42652..43935)
                     /locus_tag="IWM23_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9883084.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43949..45634)
                     /locus_tag="IWM23_000412"
     CDS             complement(43949..45634)
                     /locus_tag="IWM23_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAP9883085.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45909..46934
                     /locus_tag="IWM23_000413"
     CDS             45909..46934
                     /locus_tag="IWM23_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9883086.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47529..51182)
                     /gene="rpoC"
                     /locus_tag="IWM23_000414"
     CDS             complement(47529..51182)
                     /gene="rpoC"
                     /locus_tag="IWM23_000414"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAP9883087.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(51261..54878)
                     /gene="rpoB"
                     /locus_tag="IWM23_000415"
     CDS             complement(51261..54878)
                     /gene="rpoB"
                     /locus_tag="IWM23_000415"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAP9883088.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55606..56574
                     /locus_tag="IWM23_000416"
     CDS             55606..56574
                     /locus_tag="IWM23_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9883089.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56592..57497
                     /locus_tag="IWM23_000417"
     CDS             56592..57497
                     /locus_tag="IWM23_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883090.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57494..58300
                     /locus_tag="IWM23_000418"
     CDS             57494..58300
                     /locus_tag="IWM23_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883091.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58457..59425
                     /locus_tag="IWM23_000419"
     CDS             58457..59425
                     /locus_tag="IWM23_000419"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAP9883092.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59742..60266)
                     /locus_tag="IWM23_000420"
     CDS             complement(59742..60266)
                     /locus_tag="IWM23_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAP9883093.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60553..>61688
                     /gene="scm"
                     /locus_tag="IWM23_000421"
     CDS             60553..>61688
                     /gene="scm"
                     /locus_tag="IWM23_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAP9883094.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSD"
BASE COUNT        18711 a        12389 c        10331 g        20257 t
ORIGIN      
        1 ggaagtttat cttctggcaa gcttacgccg tctttcactg caaagactgt tgttccactt
       61 tcaatacttg ttccttcccc tgcggtgaag gttactttgt ggtagcctgc aggaatgttt
      121 tctcctggat tttcataaat atctttactt acagttactg ctaaatcggt cgtgcctaca
      181 gaaccatctt ggtaaacaat tttaatagga attgttccag taatcggctc cgattctcct
      241 acacctttag ctggagtgaa tgtaacaata ccagtgtttg catcaacact tgcattaccg
      301 aagtacttat tatagacatc tgaaattgaa aaggcatctt tagctaaagg aacatctggt
      361 ttatcaaatg taggttttcc actttgaact gtcgtttctg gagttgttgt tataggtacg
      421 tatgttgcat cattttccat agcttgagaa gcttttacag caaactctac atccacgtcg
      481 aaagttcgat ttccgatctt aacagttaca atacctttcg tactatcagt aacgttagaa
      541 gtatcaggct ctgttttcca tgaatactct gcatcttcag gtaagttttt attattccga
      601 attgattctt tagcatctgg tttaaatcca actaccacgg tttgtttatc aaccgctttt
      661 ggtgattcct taataacggt tgaaccttct tctggtttat caaaacctgg agaaacgatt
      721 tggattttta attcatcttt cgcgattaat ttacttgaat ctacacccgt aaattcaaat
      781 tctacagtat ctgaaattgg agccccatct ttttcatttg gagcgatagt tttttctgct
      841 aatacagtac cttctttatc aacaagttgg gctttgtgac ctgttcccgc taactcgtgg
      901 atgaatactt tccccttaac tgttgtgtca acatcatata cttcttctaa ttcaggtttc
      961 ccgatatatg gattcgtgtc tccgctctct tctttatcgt cagggtttcc atcaccatca
     1021 atatcttgca aataataata acctgttcca taagtgttct ggattaattt tttattcgta
     1081 tccgacaacc aagaatcaaa gatgaaatct tcaccaaatg gtttattttc ttcaacttgt
     1141 gctgttgctt tcatatcttc taagatttca tctaatggct tagtcaattg taaaacatat
     1201 cgaataacga ctggacgtgg acttgcatta gcaaaatcga taagtttagt taaatcatta
     1261 ttagttattg aacctatgcc ataattttca tgaccttccc gtcgctcgat tgctaaatca
     1321 gctacttttg ttccggattc aaacgaaaca tcatatgtta ctttgtcagc ctgaaccatg
     1381 tgcatctgta tacccgctac atatggaact aatcgttcat caattttgta attagcatac
     1441 caaggcgtgc cctttgcgga agatgaatat gagaaattat aatttttcgt taacttatga
     1501 tctaagatta tagctccatt agcacctgtg gatcctgaac catcttttgt ttgagtatta
     1561 ggtttctcat attttgcact tactgatcca tgtttaaact ggtcaggatt attttcagat
     1621 acatctaagc tattatagaa atcatctgaa tctgttaaaa agtagcccgt actatcaata
     1681 cccggtacaa ttttatcatc gtcttgatga cttaccaagt agattcgata cattaaacga
     1741 tcggtcttga gattgtctat gcctatctct ttaaaaattt catctaggct cttttcaaac
     1801 gtaattaccc cattaggagc ggtttgcgta tcggttgttt ctgccccagc aaataatcca
     1861 tcacttgcac gaatataatt gaccttccaa atatttgtag gtctacccag cgaatcatta
     1921 attcttacaa aggtccgtct agaagatcca attcctttct ttggatcgac ctgaattttt
     1981 tctacatatt tagctaatcg ttcatctaat tgaagacgga tttcccagtt tttagttgat
     2041 gctgcaatat tatgttttcc gtaaacttca aatccaattg tattgccttt tagtgtgtct
     2101 ccagttagtt cttgaggact aaattgtaaa tccgaaaagt tataattttt cgtataacca
     2161 ggattctcct gaaaagtatt ccccgaagct actttgtcac ctacttgtac ggagcttatc
     2221 gctgtttcaa gtgggctgct tggagaaatt tcatcttgtg ctttaactag ttcagttgtt
     2281 cccaagacaa gaccaacacc aatcaaaaca gatgcaaccc caacacttaa ttttttgata
     2341 ctgtacttca atactcgttt ctttgttttt tctagaaata ctctcttatt attcttgcta
     2401 accattattc aaatcaactc ccttaataat tactttccta gtaagaataa aagataaatc
     2461 tgatacgtat tattcacaga ataggaatct tactaacctt atgttaacta atttacttag
     2521 agaatttcat gaacattaca acaaaaaaag tacatttctt catgtttttt tcggatattt
     2581 gtgcttttta accattcagc agctgaaaac atataaaaag atatgtatgt attctttctg
     2641 aaattaagtt catttctatg cgatgaagca tgttttcaaa atattaccct ttcttagatt
     2701 aaacttcaga gtattcaaat aacggaataa ggagccttat cccgcattac tgaatgagac
     2761 tgtgacataa gtaaaaaggt gacttcaaat tccttcttcg gaatttgaaa tcaccttctt
     2821 tataattata aagtatcggt ttcacagcat ttctgaatga ttcctagaat cttaaacgaa
     2881 tgattggagt tatcaacatt ttttcactcg tttttagcga aacaaaaaat gacatttgtc
     2941 acagcctcat taaattattg attctttttc tttataattg aattgtccat atttaaagtg
     3001 tctagtactc tacatgaata attaattgct ttcactcaaa tacttataat ctattaactt
     3061 gtagttgttt aacttttctg aaaaagttag acccacgtaa taatcttccc agctttctct
     3121 ttcaattaac tgatccaatt tttgataatc cataaattca tcatgtatca aaaagaataa
     3181 ataacttgat attttagcat ctagcttaat cagattaaat aatgtttgtt taaaattgct
     3241 accattaata tgactgatag attctctagc ttcttcacat aaacttttta ctttattaat
     3301 tactactttg tcatattcat ttaacacact attattacca ttacttagac gctcagattc
     3361 tacgaaacaa ctcaaatcag aaatttccca aaaaaatttt ttgttaaaat ccaatttcct
     3421 caactcctcc aaactacgta aatgatatag tctcataata aaataaaacc tctcagctaa
     3481 tgttgttgaa attgtccact aaacctaatt gatattgctt aggagataca cctgcgtact
     3541 ttttgaatac tctcgaaaag tgtgaagaat ctgaaaatcc taaacctaaa gaaacttcac
     3601 tgatagtatg gtttgatttc aacattattt tagccgtaga aatttttcgc ttagagatat
     3661 aattataaat cgtaatatgc atttcattct taaagctttc tcttaattta gtttcactta
     3721 cagaaaaata attagctatt tcctttacag taattctttt atagatattt aagccgatga
     3781 attgaacgac agaagaaatt aagggagaaa gatgctctat cttagttttt ccgatttcct
     3841 tagtataata aacaatccca gaatccctca cttttaagac ctcaggtaac gatacagcta
     3901 attcgttttc ttgtatcaaa gcatctctac tttgatacgc ttctattata tccattccta
     3961 atgttataga cgtttgtgct aacttttcaa aaacaatgat agaataattt ttttcagacc
     4021 gaatcgtatc tccactaaca cttggaatta ttccattact cagattaaaa accatgtttt
     4081 ctaaatttcg aatatctcca tttttaacat attccagtat tttattttca taaagaaaca
     4141 gatatatttc tggatcgtaa ttttgtgata acagattatc tattttgatt tgctcgatag
     4201 ttttacttag attttctgta tattcaatta acggttcatg gaatagatct tctatttttc
     4261 cagtaaaaaa ataatgaact aaaatcagaa tatctcgaat gtctcctaaa gaatacaaag
     4321 tcaaattttg caatgaatta tagagtcgtt ctctatcagc cattgcaaca ttcaaattgt
     4381 tcatttttag tttaaaaaaa actttatcaa tattatttgc tctaaatgga ccaaataagt
     4441 aatatctggt tttaaacatg tatagaagaa acatctcatt aaaatcacca gtaaagaaat
     4501 ggaaatcttt agttttttta atattatcag ttagtaactt tttataatca taaaaaaaat
     4561 aagtagttct atctgagcga tactctttta taatactgaa ttcggaatta aaaaccacga
     4621 taggtaactt taaacaaaca tgaagagact ttaataagac ttctttatct gaaaacactt
     4681 ttgtttctct ctttcctttc tacatcatta aatagtattt tatacgttat ctaaaatact
     4741 attcctctgt caaaataagc taacttactt cattataaaa tatccactta tcgctttcat
     4801 gtatattcca ccgtaaaaag tacattttat caccgagaac tcatcatttt tcatcatgat
     4861 gaatactatt tacatatatc tagttcaaag acaataatta ttatgatccg ttattatcta
     4921 agcactatac agataatagg aatttttcgt ttctaaatat tccaatttgg gaatccaata
     4981 aaaattttcg ttcaaatacg tgtctattcg aactcttttc tagttgttaa ttatgaaggg
     5041 ggaagaaata aaaaatacaa tctatacacg aataagaata ttatccaaaa ggaggtttta
     5101 gaaaagattt attatacgta aaaagctcaa gacattttct tagccataag gacatgtttg
     5161 atatttaaga agatatatga attttctttt ctaagtataa agataactat ccattatcaa
     5221 ctaaaaaaac atttttgaaa ataaacatca gcagtcctat caatgcatgt gagcttatag
     5281 aagttattcg cagcgcagca taattgcatt agcttctttt cgtgtttttt gcctcttttc
     5341 cctttaaatc ttaatggagg aggtgtgcaa tatgtcagat aacgacgaac gcgtaaatgc
     5401 ccagttgatt cgattctttg aaaaggtcat tttgaacacc gctaatactt attataaaaa
     5461 acaagaagaa attcaaaaac atgaacaact tgatcaactc ccaccatatt tgtttactaa
     5521 agatactata aaatttaaac aaccgattaa tgttttaaat gtgactctcc ttgttgaaga
     5581 tataaaatta gcaaaaatcc ttcctttgtt gaaagaacct gaacaaattt ttcttatcga
     5641 aaaatttatc tttgataaaa ctgataaaga aattggaaag gttctgggga ttacaagaca
     5701 gggtgcgaca aatttaaaac atcgacttta caaaaaaatt caaaaaatgc tggtttaata
     5761 tctgcataat aactaatttt gtatgcttct ttgctcgttc tcctccttcc caaagaaata
     5821 gattataagt ccatgggaag aaggtagatt tatgaattct ttaaaagaag atttcatttt
     5881 agcaaaggct ggaaatgaag aagctgttga ggcaattctc aaaagattta gctctttgat
     5941 tcataagcaa tcctggagaa ctggaaaata tgatcaagat tgttatcaag aatgtatgct
     6001 tgcaatatat ctagctatct cgaaattcga aattaaagaa taactcaact aagggagtct
     6061 gtgacataag taaaaagata gtttcaaatt tcgaacaata aatttgaaac tatcttcttt
     6121 ataattataa agtatcgatt ttacagcgtt ctataatgat tcatagaatc taaaacgaat
     6181 gattttaatt atcaacgttt tttaattcgt ttttagctta aaaataactt ttgtcacagt
     6241 ctcactccta aaattacttt atttacgtat gaagtgggca ggaggtcgaa tccagaactg
     6301 ggctatggta cttcaacgag gacttctcct ctagaattga aaagcatgcg atttaccttc
     6361 cgtaacgacg gtagatttat tcaactatgt gtccaatcaa aatttgtctg atttcaaaaa
     6421 tagtcaagag aattttggtt gttaaaaact cttatctatc aacttccaca gtttagttga
     6481 caaaccctga tactaagttg ggctatgata cacttttctt gactatcagt tacgttaccg
     6541 gaaggaatga actctattga gaaaaacata tgatcgtaaa tttaaactaa agatttcaca
     6601 ggatatcctt gagaaaaaag taactaccaa gaaaattgct gaagaataca atatctctcg
     6661 tccaacaatt tcccgatggg tttcagaata tcgtcgatac gggaaaaatg cttttgctgg
     6721 acaaggaaaa agattacctg ataaagcaga tttctacatt cttgaacagg aaaataaacg
     6781 actaaaagag gaagacgagt gttctacgaa cacaaagatc gatacggatc tccagggatc
     6841 actcaaaaac tttacgatga aggaatcgaa atcaataaac gtgttgtagc tgttcttatg
     6901 cgtgaaatga atttatgcac aagaggattt catcgacgca aatcttctca tggtagagaa
     6961 aaaacaatca aagaaatggt aaaaaaatgt tgaatcgata atttgaacag aaccaaattg
     7021 atgctgtttg ggtgatggat attacttaca ttccatgtaa tgacggtcgc ctctatttgt
     7081 ccacctatat tgacttagca actcgtattc tacgttacta tatggtagat tcacacatga
     7141 aaaaagagat tgtcgtccgt ccattacaaa tttattaagg agaattgcct acggtaattc
     7201 attccgatag aggtagccag catcggtctt ttatttatca agagctgttg aacgaccacc
     7261 acatcacaca ttcagtgagc caaactggga ctcctgttga caatgcagta attgaagcgt
     7321 ttcatcgatc aatcaaatga gaattaatcg actccaacaa atataaaagt aaaatagaga
     7381 tgaaggtgtt aatccaagat tatttagaag actactaccc aaacaaaaga atccatacaa
     7441 aatttatgat gactccttga caatttgaag cgaagaaaat aagggatcta taatatctag
     7501 tgttggtgac gccttctgtt gccaatactt tttctttttt ctaataaacg caaacagaca
     7561 ccttaaaatc ctttagaata aagtcgacga aaaccattca aaggagtgtt tcaagatgtc
     7621 ctattcagag tttaccaaag agttactaga tattctagac ctaaatctta tctttcatga
     7681 agatgccttt cgcaaagagc gaatcaatga cgaaacatgt tttgtttttg atggcacatt
     7741 gacctatcaa ccggaggaat gctttcatcg ccactatcaa aatgaacaaa cgatcattaa
     7801 atagggctgg aaaaaagtat ccatcttatt gaatgatgtc agtaactaca agaccattct
     7861 gcggattaac aaacagcgct tcaaatgtaa acactgtgga aagacttttt tagcagaaga
     7921 ttcagttagt gatcgtcgct gctccattgc tcgcagagtc aaacaagcca ttcttgagct
     7981 attaagcgaa cctctttcta tgtctttgat cgcacgtatg aagcacattt ctccgaccac
     8041 tgtcattcga atacttcgca gccttcggcc taaaaccatt tctttgagcc agctgttacc
     8101 agaagtggtg tgtttcgatg aattcaaatc agtgaaaaat gtctcgggag ccatgagctt
     8161 tgtcatgatg gacggcaaga cccatcaact gattgatatt gtggaaaatc ggcaactcaa
     8221 tcctttacgc gactatttct tgcgctaccc tataaaagtc cgtgaacaag tgcgtctagt
     8281 ggtttcagat ttctatacac cctatcgcac attggtgaaa gagtgttttc cgaatgcccg
     8341 aatcgtcgcc gatcgctttc atattagcca acacattggt cgggctttta ccaaccaccg
     8401 aattcaagta atgaagacct ttaaaaaagg ggatcgtcgg tcgaagcact taaagaaata
     8461 ctggaaacta cttcaaaaga atgcgtggga attgaaaggt cagcatcgct actggcgtcc
     8521 gtcatttcga gatcatctga cagaagcaga gatagtcgat cggctattat cttatgatga
     8581 ttccttaaag cgaggctacg aagtctacca ggattttctt tccgttatca gaagacaaga
     8641 tgttcctgaa ttcgtcgcgc tcctaaaaga agactacaag gagttgccag agcactacca
     8701 accagtgttc acgaccttca agaaatacca aacagaaatc aaacgagcgt tgcgcgttcc
     8761 ctattccaac ggccctatag aatgtttgaa taaccatatc aaagtactaa aacgaattgc
     8821 ctatggctat agaaactttc aaaactaccg agaacgaatc tttttatatc gaggaaaata
     8881 ttttaagaaa actaagaata ctacccaact gactaaagcc agaacaacaa cacggctaga
     8941 caagatagct gtctagccgt gtgagtatct cgattattct attgaatttc ttcaccaaca
     9001 ctcattgacg aagagccaaa ataagtaatc taaccgactg ttaactaaac cattgcaggt
     9061 cccgttaaag atacttgtgc ctgttgtcac cgcttctttt tcttgtccac aagcgcttaa
     9121 cacacttaat aaagcccctg ctattattaa taataatatc tttttcttat taaaaacctc
     9181 ctcatctatt tccagattag taaatgtagt aatctgtgct ttcttttttc tcaatactaa
     9241 taactaactt atggggtaaa caaatactcg tttgaccggg ttttgagatc catccagttc
     9301 taaccgcgat ttgatcagga ctattatctt ctttaactcg aattcttcca tctttaatct
     9361 caacaatatt atattgtccc ttcgcaggat gataattcac gagtttatga ttgatcttat
     9421 ccaaatcgaa gcgatccatt tccttgccat caattcgaac gatggcatat ttgatagcat
     9481 cttcagttgt ttcattctcc ttttctatag agaatacaaa taagggaata aaagaggtaa
     9541 taatcaaaat gccaataatt atgacatcgc ctatcttgac accagctttt cttaaaaatt
     9601 tcattaacga tatctggcta aacatattag actttttcca tggaaatggt gaaatttttc
     9661 ggaaattatc tgcattaaga gaatcagtta aacaaccctc aagttgttcc ttaatacatt
     9721 gttgatccat cttcatacca ataaaaacta gtttattaat gcgttcacca aacaacggat
     9781 gccaggcttc cttcaattcg ggattttttt gtataatcat ttgacactca attttaggta
     9841 aactagctac ccaataattt aatgattcaa tacttttata gctacccgat gtttcaaaga
     9901 tcgtcgaaaa atcactatgt tgggcaaacc aaagaaaccc ttttgctctt gtaatattgc
     9961 caggaaaatt tttatccgta agatgggcaa atttttgtgc gtcaaggagt aaccgatttc
    10021 gatagataaa acttgagata ccatattctt ctgtttcagg aatatgattt tgatagccaa
    10081 gctctaactc tttgaaccag cccactgaat aggaagtctc atctatatta aaaagatttg
    10141 tatttagaat tttcgataag tctacttcac ctttaattgt cctaattatt ttggcattgg
    10201 actgtaactt agcaattaga ccgttaattt tgtaaatcgt cttggttgac actaagtcac
    10261 tattatttaa aagtaatatg ttacaacact cgaattgatc aattaacaag ccacgaatat
    10321 cctgttcttc ttcaactaca ttaccttcag cttttctttc ctgaatcttt tcacccgttt
    10381 cgaaattatc ccaaaaacgt tttacatcca ctactataat aatggcacca atctctaatc
    10441 gttgggtcaa atcaatgtct aaagggtttt tagctaatat aattgcttga ataattggaa
    10501 ttggtttgct gattcctgaa gcttcaataa caatttgatt caagtcagaa aattgcacta
    10561 attgatctag ttcaacaatc aaatcttcgc gtaatgtaca acaaatgcat ccattcggca
    10621 tcgaaacaag tttttctcca gttcttttaa aacctttctt gtcgactaat accccatcaa
    10681 catttacttc ccctatgtca tttacaatga cagcaatttc ctttccgtga tcaccgtaca
    10741 gaatatgatt tagaagtatc gtttttcctg aaccaagata gccagttaag attgtaatag
    10801 gtattttttt catagggaac ctctttctat accttgaatt tacactttaa gtgcaacaca
    10861 taaaaatgag tcataatcga tatccaacta gtataagttt gtcgagaaca ctcctagaag
    10921 gaaaataatt atgagccagt atcgtcatct tactatactc gaacgagaaa gaatctttct
    10981 gcttcacgaa gaaggatttt ctattagtat aattggcaac gacattcagc atagcccgtt
    11041 tactatttta catgaactga gaagaaacaa gaaaaaaact attcaccttc caaagcggaa
    11101 aaaaattatg cttatcgaag aaaaaaatgc ggacgttatc acattctaaa tgatccgtga
    11161 gtttggtcaa ttgttcgtca tttatttgtc gattttcagt ggtcgccaga ggaaatctcg
    11221 aatcgtttga agcatgaaca tatggacttt tcaattagtt ttatcacgat ctaacgtgca
    11281 atttgcaatg atcttttcga tacagaaccc ctctcacacg gcaataaagg cctgatccgt
    11341 tcattgcacc atcgaggaaa aacaagacat acaaaaaatc atgtcgagtc tcgtggaaag
    11401 atttgcctta ctcattcgat ccatggccgt ccaaaatccg cagatgatag aagtgatttt
    11461 ggtcattgga aagccgatat tgtagctggc aaaactggtg gtacttgttt agtgactttg
    11521 acagacagaa aatcatgctt ccttttagca ggaaagaagg tccccagaaa gaaagccgct
    11581 tatgtgagcg agaaaatgat tgaactttta agcagtattc caaatgaggc ggtcaaaacg
    11641 atcactccag atagaggaaa agaattctcc aatcatttcg cagttacaga agcattgaat
    11701 aatatgccct tttattttcc agatcctcat gcccttggca acaatgaaca aattaaaata
    11761 cgaatagact cattcgagaa tacttgccaa aatcgaaaga gatagctacg gtagataact
    11821 caacgataaa tcagatgatt gaaaaactga ataacagacc tagaaagtgt ttagattgga
    11881 gaacacctta tgaagtcttc tttgataaag tgttgcgctt gatttgacaa ttcaagacat
    11941 aataaaaagc agatcaaatc caaataaggg aaaaatgatc tactctggta ttatcaatta
    12001 attggccaag ctattatgcc gttttgctaa acgtctactt cttccagtat tgaccttgat
    12061 aaaacaacaa tcaggatccc gttttaatac tctatgaact gtgtcaatac taacatgtaa
    12121 agttgcagca atatcagcta atgtactccc atttaatctc atttgctttg caaatcgata
    12181 acgccgataa gcatcaatat tcttggaatc catagacaat aatacttttc ttaccatgta
    12241 tttcctccaa aaattgaatt ttaatgagcc atcatttctt gagcaatttc atgtttagac
    12301 aatgaagctg gatagaaagt aggccaatta tgcaattcat tatagagttt ttcttgactt
    12361 tctccaccaa agaaaatatg gaaatgctct gccttacttg gtgcaattcc atgatcacta
    12421 aattgaatat atttaaaacg accagcacct gcatcatcag tttcaaaatt aaaacgaaca
    12481 ccacggtttc ctttttcata attcaaaatt ttgtatccaa catatttgta tgtgtattga
    12541 tgatgttcac cgttgactac aaaatcaatg gtgttgtctt taatgttgat agtatcaata
    12601 tctgtcttat agccagtagt ataataatct ttgtactcgg cagctgtcat gtctttattg
    12661 atttttgctt tgtaatcgaa aacttcatcc aaaattccat cttttaacaa cggataaaca
    12721 gattgccatt cgcctgtata atcactcaat tcgcgatcct tcacatcggc atctttaaag
    12781 tacccattag caactgtctt ttcatccttc gcttcttttt ctggaactac ctcttcacca
    12841 gcaaccatag ttgttttttc taacgctttt agattgtctt ccattactga aacataatct
    12901 tcaccattat ccatttgttc ttttgtaaga ctttcaagag gattcagtac ttccaatttt
    12961 atcccagctt cgttggccaa agtttttgca attttatcat tagcattctt ttcaaagtaa
    13021 atataattga tcttattttt tttaacatac tcttttaatt cagctaatcg accagaagat
    13081 ggctcttctt caggacttag tccagcaata ggaacttgtt tcaatccata atccaaagct
    13141 aaatatccaa aggctgcgtg ttgcgtcaca aaactttttt gtttcgcttc tttaagacta
    13201 gtggtatagt cagcatccaa ccgtttcagt ttagtcaaat acttttcagc atttgtctca
    13261 aatacttttg cttttttcgg atacaattta actaactgat ccttgatatt tgaaacttcc
    13321 ttaattgcca ttttgggtga aacccacgta tgaggatcta gttcatgatg gtgatcttct
    13381 tctccgtgat catgatcttc ttcttcacta cctggcaata aaatcatgtc ttttgttcct
    13441 tctactacat taggcccagc tttcttccaa ctttctttag cttttggtac ccatgattcc
    13501 atgttttcgt tatgatatac aaaaacatca gcatcatgag cttttgccat atccttagct
    13561 gagggctcat agtcatgagg ttctgatccc gctggaacca ttaaatccac gttcgcttca
    13621 tcgccgacga tgtttttggt gaaatcatac attggataga atgttgttac aatttctaat
    13681 tttccatctt tatcattcga tacttttgca tcattagtgc tggagcaagc tcccaataat
    13741 cctagagctg ctatcccaat aagtcctaca ctgattttct tcattctttt tccttcctta
    13801 attatgctaa tttcttcatt ttatttcgtt tatgttcttt gataattttt aatgcccttt
    13861 ttctagtttt aatattcggg cttttaagtc gtcgatatgc actagatata tccgttttct
    13921 ccattagaaa ttacccccaa tcactactta tcattatttg ctttccgcca gtaatttatc
    13981 aaaactctgt ttgatccttt cttcatccaa catttttccg attagaataa tagtggtttg
    14041 gtctttttgt cgagtggtgt attgaaaatt aacctgttcg tttacctctt gcatggcaac
    14101 tataaaatcc tgctcttgta aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt
    14161 ggttaaaatc agccagtcaa tccattggag taaaaaatct ttttttatgt cttgatctga
    14221 agttaagaga attgatttaa actcatgtac ttgatggtgt tgatgatagt atttgccatt
    14281 atagtgattt tctgatgaat cactttcaaa aactaaggga tgattaaatt ttttcaaatt
    14341 ataaaaatcc tttttttgaa tcgtttcact atgtgtgaac attcgaatat cggataaagg
    14401 gttaatactt ttgatttctc tctgtaattt ttttagtgca gataaattca agttttgttt
    14461 tctagacaca aaaatgcgat ctgccatgac aagttgctta agggcttgtg gttctctttc
    14521 aataagttca aaactgtcac tatcaataac agtcaataaa ctatcgatat atactcttcc
    14581 tcttagttga ggcgctgtta aaagtgtttg aatgattggt tggggatctg ctatacctgt
    14641 agtttcaatg atgatttgtg aaattggttg ttgatgttct tctattgttt ccagtaaaga
    14701 aatcaatatt gctaataaat cactgcgaag actgcaacac atacatccat tatttagttg
    14761 taacacccgt tcctcgctat gaactaataa tttgtgatca atcccaactt caccaaactc
    14821 attttcaatt ataataattt cttccctggg gtagggtgag acttgcaagg cttgattgat
    14881 tagcgttgtt tttccagccc ctaaaaaccc agaaacgatt gtaactggta tgcccatttt
    14941 gtcacctttt taccacttag ctatttcact tctttaaaaa ttgcccgtct gcgcaatttt
    15001 ggcgaatact tttttaattc taaccgttca gggttattcc gtttattttt gctggtgaga
    15061 tacagacgtt ctccagtttc gacacattcc aaaataatat tttgacgcat aaatcttcct
    15121 ccttcaatac ttatcgatct tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta
    15181 actcgccaga ttccaattgt gaaactaaat tttctttcat atcaacaggt atctcccaat
    15241 aaccatcgat acattgaaat aactcctcgt catactcttc aaaaattgga tagattctta
    15301 aaaaatcatc aaatgataag ccttggtcct catagtaatc tttgactaat ccattttcaa
    15361 ttgcctgttg ggtcggtaaa cccgtttcag taataattcc tctctctacc atcgaacgat
    15421 aaaaagtgca tttatctagc gattgctggg tcgtttcaag ataatacttt aatccttctt
    15481 caactaaagg tgggtaaata atttccattt tgattccctc ctaaaagtta tatattaaac
    15541 tattttgaag ctcctttaat tactttcttt ccattatagt acccttttaa agatacatga
    15601 tggctacgtc tataatcacc attcgcttcg tcaaatgaaa tttctggttt tactacagtt
    15661 tgatgagttc ttcttaaccg tttcttcgcc ttagatgttt ttcttgctgg tactgccatg
    15721 atatttcctc cttttaccaa cttgcttttt ttactccagg aatcaaccct tgatgggcaa
    15781 gttctctaaa cttaatacgg gacataccga actttcgcat ataccctctt ggacgaccat
    15841 ccgtctgatc acgaagcctc agacgattcg gatttgaatc tcttggcaat ttagcgagag
    15901 cggtgcgatc tcctgcggct tttaattcct gacgtttatc tgcatacttt tcaatcatcg
    15961 ccatttgttt ctttgcttta gcaattttcg acttttttgc catatacctt tatactcctt
    16021 ttattttgat ttagacacct caaatcgtaa tcgttacgtt ttgacactga tactttacca
    16081 acaagagttg ctcttgtcaa caataaataa catgaaataa aactcatatg aatagataac
    16141 tatttgaaaa cgaatttatc ggtacataga gataatcaaa tcatttaaaa gaatatgcag
    16201 tttaggctct gaaaaataag aattcacttt tattagtcca atcaaacaca aataccgttt
    16261 taagtagctt ttcctctact taaaacggca atatgaaaga aactataact ttttattcaa
    16321 tcttttatgt ttaactggtt tatcgccaat tttttaaaac tttgattatt ccttatatta
    16381 taagaataga cattaagtga actaattcaa tcatactgat tctttcgtgt attcaaaatt
    16441 agatgctgtt taataaaatt attctttttg aaaaagaatt ataactatat cccaaaaatt
    16501 gtaggttaat ttaaagccta tttcatcatc gtgattgagc ttctttttca tagacgaatt
    16561 tctcagatag tttagttttc tttatgagat ttttgtatat ttctgacctt cgatacaact
    16621 cattatgagt accttgatca actagttttc cttgatccat cactacaatt ttatttacct
    16681 tttcaataga cttcaagcga tgcgatataa ttagtactgt cttatcttgt attaaacgat
    16741 ttaaactttc ctgaatacgt ttttcatttt ccgcatctaa actggcagta atttcatcta
    16801 aaattaaaat tggcgcattt ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc
    16861 ctccagaaag tgaacttccg ttttctccga ccaatgtatg ataaccgtca ggtagttttt
    16921 caataaagtc ttcgcagtta gctaaacgag ctgctttttt cacttcctca tctgacgctt
    16981 gactatttcc aatacgtata ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa
    17041 aaacaatagc gattttgcta aacaaagaag ctggagatac acgtttaata tcatacccat
    17101 caattaaaat acagccttca tcataatcat acagtctaga cactaatttc aagatactag
    17161 ttttcccgga accactagca cctactaacg cagtcacttc tccttgttta gccgtaaaag
    17221 agatatgatc taaaattggg gtattgttat catatgagaa agagacatct cgcaactcaa
    17281 tgtcaaaaga tttcagagga ctatctgatc cctcttggat actggtttcc ttcatagctc
    17341 taattcgctg tattttagga gctaaataaa atatttcaag cactgcttct ttcatagaat
    17401 cgaaggaatc ctttattttt attgcggcta gtaagtaacc aacgacgtag agtatagtca
    17461 cttcacccgt taacagtaag tgaacaccta ctagaatgac tactgctaaa ctaacatatg
    17521 agaaaataga ggataaggcc atgactgaaa aagtacttaa ttctaccttt aaatgaatcc
    17581 tttcactctc ttccattttt ttgaaaagtc tgtcttgaac ttttttagat agattaaagc
    17641 tattaatctc ttgctgcaat tctatagttt cttgaaattc ttccgagttt tctctcaaag
    17701 tatcgtaata tttagtattg gctttcgttt gggatttctt tgataacaag attaagacaa
    17761 aactaaataa cgttggcaaa ataacagcta atcccatttt gacattacca accaaaagca
    17821 tcactgaaat aaaagggaaa aacagagcca taccgccggc tttaggtatt gcatgactca
    17881 tcgcatgctc aataccttca acgtcagaca tgatagtttg agataaatct gataaattat
    17941 gttttgaaaa ataggataat ggtaaatttg acaaatcgtc tgcaatttgc actcttaaat
    18001 gcgcactttc tttataagta ctattatata agttttcata ctccctatct aacaaccaat
    18061 acatgaaaag caaagtaaca aacgatacgg caaaataaag ccaacggggc tttgtatttt
    18121 ctaaaactaa ttcgtctatc agcatcatta taatgaatgc tggtaccata ttaatgcagt
    18181 atacaaaaaa actagaaata gaagctttag ttaaagcttc tgcgccctga tcacttaaag
    18241 caaatttgtt tttataaaaa cttttcattg tcaagcctcc aatcatttgc attctcataa
    18301 ttagtgacca attgtttata cacttgatgt ttagccatta attcattatg atttccacgt
    18361 tctactattt tcccttctga caagacaaga atttcatcta cagatttaat gctggataat
    18421 cgatgagcta tcataataac tgttttttct ttcatcagat gttgaaaagc cttttgtagt
    18481 tcatactcat tatctggatc aatagatgca ctggcttcat ccataataac aatctttgca
    18541 tcttttaaaa ttgctctggc aatagctata cgttgttttt ctcctcctga cagataaact
    18601 cctttactac caattaaggt atgctctctt tcgggaaatt tttctaaaat cgattcgcaa
    18661 ccggctaatc ttagcgcgtc aaatacttta cttgctggcg cttgaggatt tgctatagaa
    18721 acgttatcgt aaatactctg attgaatagt ttcgtatctt gaaagacaaa agcaattgtg
    18781 ttaataattg tgtcttttga atagtgattt aaaggcttac caccaatttt aatctgtccc
    18841 ttatccacat tgtagaaacc tgaaattagt ttagctatcg tactctttcc gcttcctgat
    18901 tttcctacta atgcataaat tcttttttgt tctaaaacta agttgaaatt ttctaaaaca
    18961 cactcttctt tgtatgaaaa gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt
    19021 gtctcgttcc catattttag ttggtcttgc ttcatttcta aataaaggga gtctagtgtt
    19081 tcgactgcat atcttgcttc aaacaagtgc atacttacat acattattct catgaaagaa
    19141 acaaaaataa ttccacttaa aaaaaacaac atcaacaagt caacagctaa tatcttggga
    19201 gtggtaagag aagttataaa aaaggttata ggaactatca aaatgcaaac tatgccaaaa
    19261 aatagccatt gaaaaagaac aaaaggtgtt ttcccactca aagaatactt atatgcaaac
    19321 ttagcataat cgttaattgc tttattcaag gctttaaatg atgttacttt agcaccaaat
    19381 atctttataa cttgaattcc gcggatatat tctaccgttt cagagcttaa cacatctaac
    19441 gattcttgat atttttgaat aaaatttgta ttctctgtca ttttctttaa caagaataca
    19501 ccaattacta acatggcgat tagaaccaat ccaattttaa agttaatgaa aaagcctaag
    19561 gcaactgata aaacaggtgt tactatggct tgagcactat ctggaatcaa gtgagcaatt
    19621 gccatatgtg ttttagccgc gttgtcatca attgtctttc gaatatatcc cgaagaattt
    19681 aaatcaaaaa aacgaaaact tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta
    19741 gtctctaagc gaaacccaag gatatgagaa aataagccag aaaaaatata aaataaagct
    19801 cctaaagtta accaagcaac agtttgaacc gcataactct tagctaaata ttcatctcct
    19861 acaataatta gagactgaag aaatttgtaa atactgtaat atccatagac tgttaatacg
    19921 acagaaaaat cagaaaataa agtggctaac catccgtagt atttcatttt agggacataa
    19981 ctaaataatt ttttgtatac tttcattttt tcctcccccg cattcaattt cttctcttat
    20041 tcgtttacaa ctgttaatgc tttccgtaat agcgtaaatt agagatgtac aaacagataa
    20101 aaggtaaact acgttctatt ttttgtattt ttttaagact tttcttccaa tcaatacacc
    20161 aatcatagat aaacttgcta caactaacac tgaaatgatt ccccatatac ctaccgcata
    20221 gtggatcatt gcatccattg ttgcagcaga ttgtccttta tctatccaat cttgacgaaa
    20281 acgatctgta aagaaccata taggaatcca ctgtccggca gcactgccta attgcattag
    20341 agcccaagac gcaacttgag gaattgctcg atcatatcca aatacagata gaataatatc
    20401 agctaagatc gccatactaa tcgaactgat aaaccaaggt aagtacgccc cgcccattaa
    20461 cgtaaaaaat aacaataaaa caattgtgta aatagtaaaa attccttttc tagggtagtt
    20521 atagctaata taaacaaata tgattccaga taataatcca aaaatcccgg gggatatgct
    20581 gtgaccaaac gccccaccaa tactactaac cattattgaa aaaaagtaaa ttactaggga
    20641 aataactgta gctaaaccaa taaaaatata atcttttgtt ttcatatata ttctcctcaa
    20701 ttctatttcg ttatgtcatt aaaaatctgt tctatcaaag ttgaatcttc tactctaagt
    20761 tttatatctt tttttattcc accatttttt aaataaataa ttctattgca tgttttataa
    20821 agaaattccg gatcatgagt agctataact actgcaccct tttcttttaa gtctttaata
    20881 aggtcagcaa tcctttccat attaatataa tccaacccac ttgtaggttc atcaaataga
    20941 aataaattag tttttgacaa tgaagctata gaaaccaata atctttgttt ttctccaccg
    21001 gataaatcaa acggtaattg atctttcatt ttataaagtt ccattttttt taaaattttt
    21061 tcttgctgat ctttactaac tatttcattc cccagggcta actcgctcct cactgattct
    21121 gtgaaaaatt ggtaatccat ttcttgcatt actaaaaacg gcgatgacaa atttttttta
    21181 ttttcgatta tctttccttt ggtagggcga ataatcttca tgagtattct tagtaaggtt
    21241 gttttcccca caccgttttt ccctaccaaa ccaataacat ctccaacttt gacatcaaaa
    21301 tttatgtctt ttagaatatt ttcataagta atttcttcaa cttttaatac atctgcactt
    21361 accttttcga tatagggaac ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg
    21421 ttattgtttt cagtttttaa caactgaata gttgtagttt ttacagttct atttttaaca
    21481 aatagaactt gatcaattaa ttctttcaaa tagtaaaaac gatgttcagc tataacaata
    21541 gtaaatcctt tattttttaa atcacaaagt aagtcagcaa gtttcttagt cgctttgaag
    21601 tccaaatttg aagacggttc atccaatata attaatttag agtttagcga taaactacat
    21661 ccaatagaaa ttttctgccg ttcaccactt gatagcgaaa aaatgttttt atctaatata
    21721 tttgaaagat ctaataattc agctaattca ttgattcttt tatccatatg ctcttttgta
    21781 aatccaaagt tttccatcga aaatactaac tcactcgttg tatttgttgt aaagaattgt
    21841 cctctaggat cttgacttac gtagccaata ttttttgata tactttcttg tgttaaggat
    21901 tgtagcgagt tattttcaaa taaaatttcc ccaaccaatt ttccgtcata aagtcttgga
    21961 ataagtccat tcattaactt taatagtgtg gatttcccac tcccagaaga accagtaatc
    22021 aatgtaagac tgcctttttt tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca
    22081 tccttatatg aaaaagaaac attgttaaat tcaaacaatt ctttcacctc aaatcaaaat
    22141 acttaagaaa aaaatagtaa cggaaaacag gatgatatag tctaataagc tcatgtttgt
    22201 tttatagtaa gaacttcttt tcacaggaga ttctatcccc tttgttattg cattagcggt
    22261 catttcttca gcaatcatag ttgcacgtat aagaacggga actaacagaa attccaaata
    22321 ttgcagaggc tgaaaaaaag acaagcgaat gtttctcatt tttaaagatt ctttaatcat
    22381 tcttatttca gaacgataag tcggaaagaa tctgatcact actgtaatac ttaacattat
    22441 ttttttcggt aaatgtattt tttctaaaga ggataataat tcattgctag tatagacact
    22501 aaccaacaac catgagatca ttacgaccgg caacattcga aatacaatat ataaggatgt
    22561 cgcaaaaaat atagtaaaaa actctatctt gatagcatca ataattttca ttactaaaaa
    22621 cagaactgaa taaatcaaga tgaaataaat acatgtcttt attttacgaa acatcaatac
    22681 taaaactgta gcaataatga aacaccccac ttgaaatata tttccctttc ctaaaaaaat
    22741 aatgaccgga aaaaaaatat ttaaagtaat caaggttaac ggattgattt ttgacactct
    22801 ttcacctcca aaaaataaga tcgcactgtt atcttaagct gataaaaatg caccgttatt
    22861 cagataacag tgcgatctta tcaaaaagca taactgagaa caattctcaa tgcaagcttt
    22921 ttttaattaa ctcagtaatt caatccaccc aggaacagaa aattgataca atcttctggt
    22981 taatagcata gtcttttctt ttgaatagct atgcttcaat atatcataga atgtagcgta
    23041 gaatgagtaa atgtagaagt ggatttcttc ttcattaaag actaaatttt gattgatcat
    23101 tccgatagtc tttaacttat ctagacaaca gactgtcttt ttcgtcacaa atgatgtaat
    23161 tttttcaaca atatcttgat atttggttcc agctgattgg aataaaatca aatcgaatat
    23221 acgtttattc tcaaaaatat aattaataaa tggatctaat tcattgaagt tctctctcca
    23281 acttttctct atatctatat tttctaagtt taagaggtat ttggtaaact cttcattata
    23341 catattaata agcgcattaa tatgcggctg aataatttct tcaaataacg cttccttaga
    23401 agtaaaatgt ttataaaaag cacccgtagt tacgcctgcg gtcaggcaca gttgccttat
    23461 cttagtttgc tgaaatcctt ctttttcaaa atggcttagt ccactttcta ttaacttttg
    23521 gtgagttata atataatctc tggtttctat gggtacttcc tcctaataag atgaattaac
    23581 tttattatct tacacaggac ttgaagtttc aatcgttaac gtttattact gaactcattc
    23641 cttagagcta aactaatgag aaagagcaga ataactgata caaaaagcaa tcctacaaga
    23701 attaatagac tcatctaaat cattcacctt ttctaaaata atccgagaaa gatttaaaaa
    23761 aattatatcc agctattagg ataagtccta tcataatcac tccaatagcc attttcttat
    23821 cctccctaaa aataaagtct tttacttaaa accttttttg taatcgtcaa taaatttttt
    23881 ccctttatca gttaattgaa atcctatatc taaatagtcc tcatatctaa gtttagccag
    23941 catctcactt acagatgctg gactaacact tagaactttt gctaaatcat tattttttaa
    24001 tttctttttt ctttttgata attcctgaat tgctagtaaa taaattagta tagtagtcgt
    24061 tttaatttgt accataccta ccctccaaat tatttcgaaa ggccttcagc aattttatcc
    24121 aggttccatt tcatcatcgc atagtaacta tcaccatctt gtcctttttc tgcaattgaa
    24181 tcagtaaaaa tcgttgaata gataggaata ttggtatctt ttgatactgt tttcattggt
    24241 ctatcatcca cactgctttc tacgaataag gagggaactt ttgttgtgcg taatttttca
    24301 actaagtgtt ttatttgatc tggtgttcct tcttcttcag tgttgatttc ccaaatgtaa
    24361 gcagaaggca cattatacgc tttcgagaaa tatttaaagc atccttcact tgtaacaatc
    24421 atttttttat cattcggaat tgaagcaaat ttagatttag cttctttgtc tagagaatcc
    24481 aatttttcaa tatacttatc tagattttct ttatagaatt ttttattatc aggatctttt
    24541 tccgctaact gtttttcaat atttttagcg taaataatac cgttttctaa atttaaccaa
    24601 gcatgaggat cttccttccc tttctcactc tgaccctcta agtaaataac atctatgcca
    24661 tcacttgctg caaaatagtc tttgttttcc tctttgttcg catttttaac taattttgta
    24721 aaccaagcat ttcctccagt ttccaagtta acaccgttat aaaaaatcaa atctgctttt
    24781 gaagtttttt gaacatcttc aggcaaaggt tcatattcgt ggggatcttt cccaataggt
    24841 acgatactgt gtaaatcaat tttatctttt gctatatttt cggtaatatc tgctaaaatc
    24901 gaattagtaa ctactacttt taatttttcg ttcgaagcca ctttatcttt gtctttacta
    24961 tttattgaac atgccccgaa tataaaaaga gcaatcaaaa tccccagact tcccagtact
    25021 gctttccatt ttctcatcat aacgatctct ccttctttct taaaaatttt tgttttggtg
    25081 agattagaaa actaattaca aataagaatg ccgctgttaa aacaatactt gatcctgctg
    25141 ctatgttaaa actataacca ataaacaatc caagcaatga tgaacttgct ccgactgttg
    25201 cagaaataaa tatcatagtt ttcaagctat ttgcatataa atatgctgta gctgctggtg
    25261 taattaacat tgcaacaatt aatatagttc ccacactttg catagctgtt actgctacta
    25321 aagttaatag aaccatcaat agataatgat aaaaactgac attcatccct attgcttttg
    25381 ccattaatgg atcaaaagaa gtgattaata attctttaaa aaatagaact actataacca
    25441 acactaaaac cgaaacgcca attgtaatcc acttatcaat atcttgtaca gctaaaatat
    25501 ttccaaataa aatatgaaat aaatccgttg agctatttgc aactcctata aggataactc
    25561 ctagtgctaa aaaagaactg aaagtaatac caattgctgt atctcctttt attacactat
    25621 tgctttttat aaaagtaata atgatagaag ctaatagacc aaatacaatc gcaccaataa
    25681 aaaaattaat acctaaaata taagaaattg ctactcctgg caaaacagca tgagaaatag
    25741 catcacccat aagagacatt cctcttaaaa taataaaaca acctactgtt ccagaaacaa
    25801 tccctataac tacagatgta attagagcat tctgtaaaaa gtgaaagtca tacagaccat
    25861 taataaaatt ttgaagcata ttattccccc tctccaataa atatagtatc tccataggct
    25921 ttttttaaat tttcttcatt aaatacagat tctacacttc cgtgagcaat tagatttcga
    25981 ttcaaaagaa taatattatc aaaatacttc tttaccttgc ttaaatcatg atgcacaatt
    26041 agcactgttt ttccctgatg tttcaatttt tttaacgtat ccataattat tcgttcacta
    26101 actaagtcta tccctacaaa tggttcatct aaaaatatga aatccgcatt ttgagcaaga
    26161 catcttgcta aaagtacacg ttgaaattgt cctccagata attctcctat ttgatgatta
    26221 gaatattttc ccatatcgac cttttccaaa gcctttgaaa ctctttgcca ttctgcattt
    26281 ttaattcttt gaaaaacttt cattccggca tatgttccca tagatacaca ttcttttact
    26341 tttattggaa aagtaaaatc aatatcagct ttttgctcta catatgcaat cttcaccagt
    26401 tttttattga tatcttcatt attaatggag acctctccct gatgagggat aatatttaac
    26461 attgctttaa gcaatgtgga ttttccagca ccatttggac caataatacc tgttaaagca
    26521 ccttcttgta tttttaaaga aatctcgttc aatgctaaaa aatcgttata acaaacatta
    26581 agctttttca cttctaacat aactttctcc ttctaaatga taatgattat cacgatcata
    26641 tggtatacaa aaattatatt ttagtcaacc ctaaatttta tagtttagat ttttttctgt
    26701 tgctgaagat tagaatgaca aaactatttg aaactacgcg tatagtaagt agctttcaaa
    26761 atatataaaa aaggagcgtt ctcaaatgag aacatgctcc taaaactact ttattggcgt
    26821 atggaaaaat tatacttgaa gtggatagcg ggttgaataa gttgataagc atcaaaacat
    26881 ttataaaaat tattcaagac tatgtctgtt ccatatacaa atatgaaaat cagtaaactc
    26941 catttaactt ttttcgtagt gtgtcttttt atacatgttt atcgttatta atagtgaata
    27001 ggaaaaaaac tatttttgat cattgacaat taaatacaat ttttgaataa cgatccctgc
    27061 tattcgagct agaaactcaa gtacgccatg acctgattag cgacaaatac ttagagattt
    27121 ctttttgttc tgggaaaaca aaatgccatg acaatagcag caaataatga tacttccgta
    27181 aggctcgccg taaactgggg agaggtgaaa ttcctatgtg cttacccaaa gcaattcaaa
    27241 aatttcataa ctactatttt aggagatgat tttatagaat ccagagtcta cctgaataaa
    27301 aaacaaacct gtgcatttct aggaatcgat aataatactc tagataagtg gattcgtata
    27361 tataatttcc cagtaataag agttggtggt gtagttcgct ttgacgtaga agatattaaa
    27421 gactttatga atacatttaa aataacttga aattttcttc tcgccgcata tactaaaatt
    27481 agtgaaatat actgccttac tttttttaag gagtgaggat tttgcctaca aagctttcaa
    27541 atgggaaata taaaactaat ctgagatatc caaaaaggtt taaagaaata acaggaattg
    27601 catcagaaaa gtatcaaaaa acatttccaa ataggcaatt ggcgattaaa gccgaaaatg
    27661 atatgaagaa gaaaatagaa aaagttttga gagaggagaa tgcaaactca ttagaattaa
    27721 aaggaaaaat aacgtttaaa aaattttacg aatctaaatg gcttcctaga tatgaattag
    27781 gtcaaacaat tagatcaaac aggcctccta gtgatataac cattagtaat acaaaagata
    27841 tatttcggtt gcatatttta cctatgtttg gtgaatacgc aatgaattat ctaaatatca
    27901 atactgagat aatatcagat gaattaacca aaaaatcgaa agaatatgca aatataaaaa
    27961 tcataaaagg atatgttcgt tccatgttcg atattgcaga aatactaaat tatatcgaat
    28021 ttaatcgaac tactaaaatt attcaaagta tcactgcacc taaaaaaaat gctttagaag
    28081 aaaaaagaat acaagaaggt aagcaagcac tatcatcaaa agaacttact aattggatag
    28141 aagcagtaaa tgatgatttt aataatcatt tacttacttt tcatgattac acacttttta
    28201 tgattaccct gtatcttggt gatagaaaaa gtgagactta cgctttgcaa tggaaatata
    28261 ttgattttga aaaacaaacg gtccgtctta aacatacttt agataaatat caaagaaaaa
    28321 agtttacaaa aggtcgaaaa gataccgtca ttcaagtacc agaagttgta atgacattgc
    28381 taagtgaatg gaaatcggta caagctgatc aattgttgaa attaaaaatt aagcaaacgt
    28441 tagatcagta tctctttact tatactaaac catctggaga agtaaattgt cctgttcatg
    28501 ccgattacct aaactatcga atcaattcaa ttaaaagaag gcatcctgac ttagttcatt
    28561 tatctccaca caaactccgt catacttatg caactatagc tagacaaggt ggagcagata
    28621 tgaatcaaat ttcaaatgct ttaacacact cagatatttc tacaacaaag atatatgtga
    28681 acacaccaga tatagttgat aaggctgttt ttgaagcttt tcagagagga ttgaataagt
    28741 gtgattgata tgtgttctta aatgtgttct taaaaacaaa agaagcactt tccaaataat
    28801 tgaaaagtgc cagaaacgtt gatatataaa ggatattaac gttttgagaa ttgtgaagct
    28861 ttacgtgctt tcttaagacc tggtttttta cgttcgacca tacgtgcgtc acgagtaagt
    28921 aagccagcac gtttaagagc taaacggaag tcaggatcaa cttctagcaa tgcacgagcg
    28981 ataccatgac ggatagctcc tgattgtcca gcgtatcctc cgccgtttac gttaacaaat
    29041 acgtcatatg cgccttttgt ttcagtaaca gcgaaaggtt gattgattac ttcacgcaag
    29101 tcagcatgtg gaatgtattc ttcaacatct tttttattta cagtaatttt gccagttcct
    29161 ggtactagac gtacgcgagc aactgcgttt ttacgacggc ctgtgccgat atattgaact
    29221 tgtgccaatg aaattccctc ctattagatt aagtttgtga tgtctaaaac ttctggttgt
    29281 tgtgctgcat gtggatgttc tgctccaccg tatacgttca attttgtaaa ttgtttgcgg
    29341 cctaaagtgt ttttaggaag catacctttt acagaagttt cgatcaaacg acgagaattt
    29401 ttagcacgca attcgccagc agtgattgat ttcaatcctc ctgggtagtt gctgtgacgg
    29461 taataaattt tgtcagtcgc ttttttacct gttaatttca ctttgtctgc attgatgacg
    29521 atgacgaaat cgccagtatc caaatgaggt gtgaaagttg gtttattttt accacgtagt
    29581 acagatgcaa caactgttga aagacgtccc aatggaacat cagttgcgtc tactacatac
    29641 catttacgtt ctacttcgcc tggtttggcc atatatgttg tacgcacgtt attttcctcc
    29701 aatttaagtt ctgatgtttg acatacactt cgagtttccg gggctctttg tggggcaaac
    29761 aataccattt aacatattac ccgctaatct agtcaatgtc aacgtttttc tgcttgattg
    29821 aaaaatttat atgaaacttt acttcttttc tttcttttat ataaaagggt cgtttcctgt
    29881 caaaatgaaa aacaaaaaga ggctgtgaca aaagtcttgt cacaacccct tattttgaat
    29941 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atatccataa aacataagaa
    30001 gctgtttttt aaaattgttt cttgtgactc ggagctgaac ggcaggctta caacctcttg
    30061 gtaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca aaatatgagg
    30121 aactattttg acgtttgttt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    30181 tttcggtcaa gttcaacaac agtttttacg cgtcgatctc tggcgctttg cccaaatgag
    30241 cttggatcat ccagatacgt ttttctgtgg ctgttttgaa ttcgataaag atatcttgtg
    30301 ttggatcgtc gccttcttct ccagaaactt cgattccttt ttggtaaaga tctgctagat
    30361 aacgatagcc tgcaacaagt gtttccaaac gttctggaat agtacgatcc cagcggccaa
    30421 tttcatcagg gatttttgtg ttatctgcaa attcacgcaa tgtagaataa ggtgaaccgt
    30481 ctaatgtgat caagcgttcg gatacttcat ctaactgagc gttcaaatca tccatgaact
    30541 catccatcat tgggtgcaat gtaaggaaac ctggtcctct catgtaccaa tgtgtttggt
    30601 gaacaacaac agacatttgg cttagatctg caactaattg atttaatact tctttggttt
    30661 gttggaattt catctttctt gcctcctaac attttcttac acttaaaata atagtagact
    30721 ttcaatgaaa agtacaacaa aatcatttac ctgagaacga ttttcagttc gcttttttat
    30781 ccgtaaagcc aatcattttt cagaatcagc taattcttct cgcagttgag caaattgctg
    30841 ataagtctgt gctaaatgct tttgtgtcga aacaaataat ggatagattt tctgatattt
    30901 tttatgatca gtttcttgta ctccaacttg ctctttcaat tctatcaatt cctgtgcttc
    30961 actgatactt tggatctttc cgatgcttct ccagcctaaa atcacagctc ctaatgcgga
    31021 tgcttctgtg acagtaggga aaacaagcgg gtaacccagt acatcagcca tcatctgacg
    31081 aaaaatcgga gattgagcaa acccgcctgt agcacggatc tctgtgagct gattttcctg
    31141 gcaaactcct tttaaaatcc gataaaggtt gagacagatc ccttctagaa tcgccttcat
    31201 catttctttt tctgtatgtt gccgctgtaa accgataaaa ctgccaaacg catctgcgtc
    31261 ccaaagcggt gctcgctcac caagtaaata gggatgaaaa atcagaccat tggcacccgc
    31321 tggcacaaaa gcgatagccg aaagcagtgc atcatatgga ttttctcctt tttctttagc
    31381 agattgaacg acttcctgta aaaatgtatg ccctgcccaa tcaaagatgc cagcaccatt
    31441 gctagtcgct cctcccacta cccaatgttt ctgatccaga acataacaaa acgtctccat
    31501 ttctggatgg aactgaggct gagtagtaat aaaacgaagt gctccgctcg ttcctacggt
    31561 caaagcggcg atcgtatcac caaaagcacc taaccctaga ttagaaagcg gcccgtctgc
    31621 ccctcccaaa acaaatgata tatcatttgg caaacctagt tctgctgctg tttttgatgg
    31681 tagacctgtt acttgatagg tggaaggaaa gacttgcggc aattgctcta ttcgaatgtt
    31741 caagtagtca agcacttctt ctgcccattg gaattcatgg atatcaaaat atccagttcc
    31801 tgaagctgtt ccgtagtcgc tgatcagttc gccaaacaat ctataaaaga tatagtcttt
    31861 gataccgaca aaataagcag tttttttgaa taattcaggt tggctgtttt gcagccactt
    31921 gattttgttc aatggtgtca ttggatgaat cggcgtccca gtctgccgat aaaattcttt
    31981 gccaagcacg gtatatttca gtttttctgc ttcgtccgct gctcggttat ctgcccaagt
    32041 gatcattcga gataacggtg ttttctcttc atccaacaat atcaggccat gcatcgcaga
    32101 ggagaaggag acaaattgaa tgattgtgtc tgaaggcaac tgtttaatta cttcttttat
    32161 aacggtgcag acaccttgaa agatcaattc ctgatcttgt tctgccatcc cttgtgtttc
    32221 ttggatcaaa gggtagcgaa aataagcgga tgctgcttct tgggcttcat ctgtgaaagc
    32281 aactgctttg gtttgtgtgg ttccgatatc taatccgatt gaaacgttca ttttttcacc
    32341 tcaaatttta tgtatgtaca aaaatttagt ttaagcttgc gtatcttggt tttgaaaagg
    32401 gggagatggc gtgttacttt attttatcat tggttgctgt atcggatcat ttttatgttt
    32461 aaccgcccag cggattccgt taggtcactc tattatttat cctagatctc attgtgtcaa
    32521 atgctgtcgt tcgctttctt ggtatgaatt gattccgatt ctctcgatca tcgtccaacg
    32581 atttcgctgc cgctattgca ggtgtcggtt gcccttctac tatttgttgg cagaagcctt
    32641 atgtggtggt ctttttgcct ggttctttac tttttcttcc gcaagaaatt tttcgacgtt
    32701 catatggatg ctttcggctt tgctattttc gttgatggat ttattttact tcatggtcca
    32761 ttctcacacg ctattctgtt cttgggcaat tttatggatt ttttggttgc aggccggcgt
    32821 cttccaatgg caaagtgtgc tgttaacgtt tatcgtaggc ggtgtgtgcc ttcgctatgg
    32881 tcaatcctat ctcggagctg gcgatactct gttgatgctt tcttggagtg gtggcttgtc
    32941 tctagaagag ttgcttcaga tcttgtttct tgctagtctg tttggaatat ccgttttttc
    33001 tgtttacttt atctgtcata aaaaaaagtt ggaaaagctg cctttcattc cttttttgag
    33061 cagtgcgttg ctgattgtcc ttcatcttag gtattgaaaa aaggttgtga caaacgactt
    33121 gtcacaacct ctgatatata gtgttctctt accaaatcct tgtgacttgg agctaggcga
    33181 caggttcaca acctctccac taaatggtgt tcaatcagct gaccctcagt attagctcaa
    33241 aatgctggtt ttacaacctc tgatatacgg tgttccataa gtagcttttt cggcttctta
    33301 tttcttgacg ccccaatgtc gacgaaggaa acctgcacgt ccagaacctt ctcgataaag
    33361 attgtaatgg agcttgtttt tcttgtaata atcctgatgg taatcttctg caggataaaa
    33421 ttcgcttctt ggttcgattt ttgtcacgat tggcttatcg aatcgcccgc tgtcttgcaa
    33481 tgcttgtttt gaacgctctg caatatttcg ttcttcttca tcattatagt agatcactgg
    33541 acgataagaa tcaccgcgat cagcgaactg tcctaatgcg tcagtcggat cggtttgctg
    33601 ccaatagatt tccaccaact gatcataaga aataacttct ggatcatatt cgatctctac
    33661 tgcttctgta tgccctgttg tgcctgttgt tacttgttca taagttggat ttggtacatg
    33721 cccgccagta taaccggatg tcacagaaat gatccctggc tgctcttcaa atggtttgac
    33781 catacaccaa aagcatccgc ctgcgaaaac tgcttttttt gtcatataaa atcccccttt
    33841 tttctcatca taccttgatt tccaaaagga ggataacgtt ttgcttgtga cagaaaaaac
    33901 ggaaactatt atatacgtga tacggcttct gataaatgcc aaaacgaacg tacttgatca
    33961 aaaacggtta aatcaggaat cacatattca gcagaaggaa gatttatgtt ttcacctatt
    34021 cccaatacat gcatgttacc agccaaagct gcttgacaac cagcttctga atcttcaatg
    34081 accaagcatg catttggcgg tacattcaat tgctgtgctc ctttgagaaa aacttctgga
    34141 tcaggttttg cttttgatac ttgcgtgccg tcaataagga catcaaaata acttgtcaaa
    34201 ttcgtttttt ctaaaatgag gcgtgcattt ttgcttgctg atcccaatcc gatcttgatg
    34261 tttgtttttt tcaagtactc aagaacagct attgcaccag gcaaaacgga tgtttcatcc
    34321 attttttgaa tcgcttgaag atatagattg tttttttgct cagcaaaggc tgatttctct
    34381 ttttctgaga atttctgttc catttttcca aatgacagca atcgctctag tgactcggtt
    34441 ctggatattc ctttcagttg ctcgttttct ttctcagtga atggaatgga cagctggtta
    34501 gctaaaacca gccacgcttc gtaatgataa cgagctgtgt ctacaagaac gccatctaaa
    34561 tcgaatagtg ctgctttgat tttcatcgat tacctccttg aaaatgaacg acttgaagta
    34621 aagatgcgat gtttttagct gtgcgatgaa gattatcagt ggcttgctgt aaagcttctt
    34681 gtaaaggcac agctgcagga aggatcggaa aaacggccga aatgccaaca tctgctgctt
    34741 tttcgcttcc tttaccaaca cttccgcaaa tagctatcac aggaacgccc tctggtactc
    34801 ttctagcaat cccaactggc gcttttcctt ttagactttg tccatctaga cgtccttcgc
    34861 ctacgattac gaggtctgct tgctgtaccc gtttatcgaa ctccagtaaa tccaatacgt
    34921 agtcgatgcc agagactatt ttccctgaag caaatgctaa cagtcctgca cccattccac
    34981 caccagcact actgcccgat gcctcgatta cttgaggggc aaatctcata taaaaatcat
    35041 gcatggcttg gtcagccact gctaattcat tttctttcaa gcctttttgt ggtccgaaaa
    35101 caaaagaagc gccttctttt ccgcataaaa gattagtaac atcagtaatg acagtgattt
    35161 tggctttaat cagctcaata ggcacgttcg ttttatcaac tgtagcaatt ttacctagat
    35221 tttctcccag tgcttctact ttttgattat cttgatcgta aaaagcataa cctagacctt
    35281 ctgccatgcc gatgccgcca tcgttggtac tacttccgcc aacgccgatg atgatgtgtc
    35341 gaacaccttg ttgcaacaat tgtcgtatca gttctcctac tcctcgcgtg gataacctaa
    35401 gtggctgttg ttggtttagt gggacatctt tcaatccgac gatttctgcc atttcaaaaa
    35461 tcgcagtttg accgtctgaa gcgtactgga tttctttgtt tttgccaaat gcattagtaa
    35521 cgactgctgt ttgccgcttc aaccttaaac tgtctgctaa tgatgaaacc gttccttctc
    35581 ctccatcgcc tataggtagc agatcatatt cagcctgaac aaaaacttct tgaaaacctg
    35641 attgaatggc ttgtgctgcc tctaaggctg tcagactttc tttgaaagaa tctggcgcaa
    35701 tcagaatacg cattattttc ccctccgttc caagatcagt tctttttcca gctggtattg
    35761 gtctccattg acaaaaatag gtaatgtttc tttcgtctct ttttttatta ctatttgttt
    35821 ttttgtcaaa acaaatagca acctgtgatt tttccaaata aaaggaaaag aaattttttc
    35881 ccatgctgaa ggaagtcttg gagaaataga aagagttcca ttcgttactg taatacctga
    35941 aaaaccaaat ataactgcta gccatagtgc tcctaatgaa gcagcatgaa ttccgtcatc
    36001 tgatgaatgc ggatctggtc ctaaatcaat caggcacgct tcttggaata aatgatacgc
    36061 ccattccatt tcttggatac ggcaagcctc tatggcgtga atcgctttac tcaacgatga
    36121 gtcgtggatt gttttgtttt catagtaatg caaattctta gaaacgattg gttgagaaaa
    36181 atactcaggt aataagtata acagcatgac aagatctgct tgcttgctga cttggagttc
    36241 atttacttcg ctacgggcat aatcaagtaa aatagactgg ctgccaggac gttctttgta
    36301 agtggtcaaa tcaataactg gcttactgag atacgtgtca tcttgcggaa tcagctgttc
    36361 tttgttaggc actggcaaat ataacttatc taaaaaattt tgacattctt tcaaaaaaag
    36421 cggatcgcca gcttgatatt taacagctaa ggccacatta taatgcgcca gatagttcgt
    36481 atatgcatta ttgtcgatat attctgtgta ttcatctgga ccaatgactc tgtgtatctc
    36541 taaatgatca tcgactttta cagctctgct cagccaaaaa gctgctgttt catgaatgag
    36601 cgtttttccc tttgtttcta aaaacgattg atcctctgtt gcttggacat actcttgaac
    36661 ggcataagca aggtctgcac tgatatgatg ttctgcggca gctgaagcaa cttcttgtct
    36721 tgttccggtt ttgatattca atgcagcaaa tttgggtgtc tcttcctgac cagtgtgtgc
    36781 gctttcccat gggaaaagtg caccttgata gtgataatct cttgcttttt gccgtgcgcc
    36841 ttctaatcga tcataacggt acagcagcaa attctttgct acttcaggaa actgatataa
    36901 aaagaaaggc aggataaaaa tttccgtatc ccagaaaaca tgccctttat aaccttcgcc
    36961 agttagtccc ttcgccccta cactaacact tggatcattg cggggagtca taatctgtaa
    37021 atggtaagca gcgaagtcca tcgctaactg atcgaaagaa gaatcgcctt ggatcgttat
    37081 ccgatgctga tgccaaaaat ctgtccaata agacatcgac tgctctaaaa tttctgtata
    37141 actaacgctt ttctgggctt gggcaatact tttcttaaac gggtctgttt gctgaagaga
    37201 ggtagagagc acgatgatct tctccagcgt aaaggacgta ttttttctga tcttttctga
    37261 aaaggaagcg gtgatccggc gattttttgc ttggaataca cctgctttat tgaatttagc
    37321 tgcaatccca atatcaattt tactttctgt tgtctggtag gatgcactga tcaaatcgtc
    37381 accaaatact cgcagttctt tttcaaccaa ctgctgtgta ccaaaattgg tttgctgtcc
    37441 atcgataccc gttttcacat ctacttggca gtcttcggtc aacggcttga tgaccacttt
    37501 cagggctgcc tgatggagat tattttgatc agcaacacga caagtaatga cttccacttt
    37561 ttttccttga gcagtttcaa tgagcgcata gcgtttaagc tctcccattt ctaaatctag
    37621 tgcccgctcg tatgcaagaa ctttcatcgt ctcaagagaa aaatgttcac cgtcgatttt
    37681 tatttcccat ctgatgacat ctgggagatt cactaactcg gctgattctg ctcctaaagg
    37741 tcggttatat acaccagctg caaacattcc ccttacttga ttccgatagt cttcctcctg
    37801 gcttcctctg ataccaaaaa atccatttcc aatggtcatc tgactcgcat acttattgat
    37861 atactctaaa tcaaactgtt tttcttctac tttcataagg taataacttc tcctgtttct
    37921 gccgaacgat ataaattttc caccagattc tggatgacat atccttgcca gccatctgca
    37981 atcatctgag tctggtcacc gagcgcccga ttcacaaaat gagccatact tagttcatgt
    38041 gggtcattaa ctggcgtggt cttttgtaag gaaaccagct ttccatctac atcttcgaat
    38101 agttcgcctg gatacagatt agcacctgcc tgatcgccac aaaaagccac attcagtttg
    38161 tcttctctga tattcaaagc aaacgaagta ttcagttgga tgatcttgcc gttttctaat
    38221 tccagtgtgc caaaaattgc atcttctact gtgaatttgc ttggatccca tttcccgaat
    38281 ttccctgaat tttttttcgt accaattttt tggaagctgt acgcccgaac gctttttact
    38341 ttaggaaatc cagtaatata catcgctgta tcaagcatgt gaatgccata atcgatcaaa
    38401 ggcccgcctc cttgaagttc tttgtcgata aacgttcccc agcctggaac accagaccga
    38461 cgaagcgcat tcgcttctac gtaataaata tccccgagaa catcaagatg attcttgatc
    38521 attgttgctt cgtcagaaaa acgatgctgg aatccataag ccaaaatctg cccgttttta
    38581 tcggctgtct ctgccatctt tcttgcttga tcagctgtca tagctggtgg tttctcgcac
    38641 atcactgaag cattagcttc gagggcagcc aatacatgtt cgaagtgaaa gcgattgggt
    38701 gtacagacac tcacgatatc gggagaaatc tcattcagca tttgtgtaac tgatgtatac
    38761 gcatgagaaa atcctgtacg tcggcaaaag tcttctgctc gctggctgac tggatcaacc
    38821 acagcggcca attctaagac atctgatctt ttttgataat aagcagcatg tactttttct
    38881 gctacttgtc cagcgcctat gattgctact ttcttcattt atttctctct cctttgctta
    38941 tttacttatt gttattcttt ctctattctt tctctaaatc atttaaatga cgcgctgttt
    39001 ccagacagtt tttcaagtat tgcaatgatt cctgataaat tttcacttca ttttctcctc
    39061 gcaaacgaca ttcatatacc atataacctt catactcatt ttgaagtagc gtttgaaaat
    39121 ggcgagtaaa atcaattgcg ccagatcctg gttggtagcg atgattatct gctaaatgga
    39181 tatgacccac atactctttt tgcgtagtca atgcttttga aatatcatct tcttcaatat
    39241 tcatatgata aaaatcagcg atgatctgca catgtttcaa ttgattagag tcaatgtaat
    39301 gacgcgcatc cgctaacaaa ttgatcatat ggtcttggta tcggttcaaa ggctccaaat
    39361 aaataaccgt acctgtttct tcggctactt tatctaaata acgaagtgat tcgctgactg
    39421 ctttatagtc tccttctttg cttcggggtg aaaccatcgg cggtaatcga taagtgaaca
    39481 ttccccaagc tgctgggata acgattcctt tgcctccaac ctcgcttagt gcacgcaaaa
    39541 tctcagcaat ttgacgtaca ccattaagac gccgttcttc gataaagtca ccgatccacc
    39601 catcgtagcc gttacaagct gtcactactg ggatccctgt ttctttgatt gcctgtttga
    39661 ctgtgtcgat attttctatt aacaacttac cgtcgatttc gtattcttca aatccacagt
    39721 ctttgataaa tctaaatttt tctaaaatgt ctcttgggaa aaatgcttga tcttgcgtac
    39781 cgaatttcat tgttctcctc ctcggacttg aacgcccatt ttgatacttt cttctgggtg
    39841 ctggtctaca taacgcatgt aagcttctgc gctttctgca aatggcacaa ctggatcaat
    39901 gatttcttca caatccaagt aaccgttcat caataattcc caacatgtgt cctcgattcg
    39961 tttacggttc catcttgggt aatctgggtt tggttcacta gatgctcgag caaagacgat
    40021 ttttgcatta ttgaaatgtg cttcgcgacc aagattaaac ccagctggaa aaggtttagc
    40081 aaaggcaaca taagaaatga tcccgccata gccgatccct attaaagaag cttgtaatgc
    40141 ggcagcactg ccactcgttt caataactga atctactcca cgtttatctg tcaattcctt
    40201 tagacgaaaa ccgacatctg ctttcatagg atctagcgtg taatcggcac cgtgtttttc
    40261 tgctaattct ctacgctgcg ctatgggatc gatcccaacg atggtagtag cacctgcttt
    40321 tttagctaat tgaatcgcaa tttgcccgat agcgcctaaa ccgaagattg ctacagcatc
    40381 acctgctcga acatgtgctt cccggatacc acctaaagcg aattgagcag gatcgtaaca
    40441 tactgcattt ttccattgat ccaaccgatc aagtttacgt agtttataat tatcaacacc
    40501 gtttgtaatc agcgtttcac gaatgggtcc ataagtgcag actaactctc ctttttgata
    40561 ctctgtcacc aaactgcccg tttcttcgat cgtaccgaca atcatatttc ctaattgaaa
    40621 ttcgccaaat accacaccgc gttggcttcc ttcttctctt ggcaagaata ggcgccattc
    40681 ttctgaaaat tcttcatcga taaatggact taatcctcta aagtcgacaa tttctgttcc
    40741 tggttttgga gtagcgaaaa ttactcggat acgtacttcg ttcttttcga tctcacggtc
    40801 ttcgtaatct ttgaatccgg ctattcttgg ttctaatgca actaattttt tcatcttttc
    40861 tttcctcttt cttttctttt tatcctttta cgccgccttc gactaaatca cctttaataa
    40921 atcgttcaga taacgcatac ataatgatga ctggtaaagc ggtcaccaat gaagcggcca
    40981 tcattcgtcc ccaaatgtag tctggtgtac tgaataatgt gttcaaaccg attggtaaag
    41041 taaatttttc ggaggtcgac aggaaaatcg aagcaaataa ataatcattc cacgaaacca
    41101 taaagcaata aacgaagaca gaaatgattc cagaaatagc caatggtata atgatccgaa
    41161 taatgatctg gatccggcta agaccatcga tcattgctgc ttcttcaata tcagtcggaa
    41221 tcgtgtcaaa ataacttttt agcatgaata tcgcagtagg aagtgtttga acgaccatcg
    41281 tgataatcag tgctgtattg gtatcataca aacctagacc ggaaatgatt ttaaataatg
    41341 gaacaacgat caaaataccg gaaaacatat aaacggtgta aaagctagca ttgattttcg
    41401 agcgagcttt gaagcgtagt tttgacaaag cgtatgcccc gctgatccca atcactactg
    41461 cagttacgga ggctatcaag gagatcacta aactattttt gaaatacatg atgaatggaa
    41521 aaatctcagc attgaaaata tcctgataat gttcaaaagt ccattctttc ggaaaaatag
    41581 tttgtgtcgt tgcaattgct tcttgtgaat ccttgaatga tgtcatcagc atgatcaaaa
    41641 atggaaacaa attgacagct aatagcaaca aaatcgtcgc gtaaaataag attttcctgc
    41701 tctttttatt tttcatttca agtccacctt ctttcgtgag agcaagacga ccgctgcaat
    41761 aatgatgaac aatagaatcg atatagctgc tgcttttcca agatcattga acgcaaaggc
    41821 tgtctgatac aaataaacgc ctaagatatt gactttattc gtcagtagat agacatctgt
    41881 aaacatatag aacatccaga tagatcgtaa aacaatcaca gttgctagta ctggtttgat
    41941 tgccggcagt gtcacgatct tgaacttctg ccaagcattt gcaccgtcca tttcagctgc
    42001 ttcataaagc gttttgtcga ctgtttgcaa aatagccaaa aatgaaataa acgcatacgg
    42061 atagtatctc cagatagcaa acaaaacgac cataaagaag ctagcaactg gctggtcgaa
    42121 ccacagcggt gcttcgttga aaagctgcaa cctatctact aacagatcat tgacgattcc
    42181 ataactatta ttgaacatat atttccaagc aaaaatcaaa gaaatagatg gcgtaacata
    42241 acttaataaa atcagtgaac gcaccgtttt cctaaatctg aacttgcggt taagcaaaat
    42301 agctacggct aatcctaaag ctgtactgcc tgctacaact aagaatgtgt aagcaactgt
    42361 taaacctaaa gaacgataaa attcactatc acttaaaatt tcaagataat tgcttaatcc
    42421 gataaaaacg gcgtctgagt taggattcag cggcatatcg aaaaaactga tctgaatatt
    42481 ggaaagcatt ggaatcagca ctaaaacagc taataaaagc atgcttggtg ctaaaagtcc
    42541 catggctaat tttaagtcag aatgtttcct tggacgattc attgaaacct ccccctgata
    42601 atctgccaat agctatctac ttttttcgaa caccattggc agaacttctt gttatttcgt
    42661 atttttttgt gcgtcagcta aagcttgatc gacatcttcg tttccgacag ttacttggtt
    42721 cactgctttt ccgatcacac ctgagcttgt gatatctccc attttcgtga aattcttttc
    42781 cccgactagt ccaaagactt gtacttcatc aaaagcacta gcaatctcag atggcagctc
    42841 gccaaaagac ttgatgactt cattttcttt gtaaccatcc agttcgacta cttgtttatt
    42901 cactggttga gcaccaccag gagacatcaa gacccacttt tccatgtttt tcggttgaga
    42961 taagaaagct gtgaatgctt cagcagcctc tttttgtttc tcgtctagac cagaagaaat
    43021 cgtcaatcca gatactgtgc catagacagc ttgatctttt tgtgttggga tagcaaaacc
    43081 gatattttct gagttccccg cttcataaac agaaggcaaa atgtaagttg aatagattgc
    43141 cattggtgct gttccattca tgaaagcatc tttgatttct gtcacatcat tagagcctgg
    43201 catcgtgtat tgggataagt tttgataaaa cgacaatgct tctctcatct tttccgtatc
    43261 gatcgttacc tcgccttcat catccaaaac atttgctcca tttgacaaag caaattgaga
    43321 gaacgcttgc tcactcattg ttccttcagc agtaggcata gcaattccat attgtttatt
    43381 ctctttatct gtgaaatgtt gagcaatttt taaaatatca tcccaatttt ctggttctga
    43441 aaaaccagct tctgataatt tttctttgtc ataccaaata ccttgaaccc agccgctaat
    43501 tggtgcagca atatagcttt ttccatcttc tgaacgaaca aggtttttgg ctccgtcata
    43561 ataattatct tctccagctt cctcgataac gttttggact gccgtctgat caatcagttc
    43621 atctttatcc atcactttag caaaatcctg actgacttcc attacttctg gtaatttacc
    43681 ggaacgagct aacgtaacga tttttgtatt gaacgctgat tcttctaccg gcacttgctt
    43741 gattttgatg tctggatttt ctttttcaaa atcagcaacc agttcattga tcacacttaa
    43801 tcgttcctgt tcgacagaag agtgcatgaa ttgaatcgtt gtctgttgtg attcgttaga
    43861 gctttgactt tcaccacaag cagtaagaag aaatccagta gctagcagac aggtagtaac
    43921 aataattttt ttcatttttt tccacctctt attttattat tttcagccac atatattcat
    43981 acggctggaa tatcaacttt cgttcttgac tgcaatggat aatttgtcca gaaaacaggt
    44041 ctaggtaatc tccttttggt aaaagataac ctgctgattg attggaaaaa ttatggataa
    44101 ctaagacttt ctcttccttc gcttcgcgaa taaaactgat aaaagaatct gctgagtcca
    44161 gtaagtaaat caaactattc ggatggaagg ctttttcttt ttttcgaata gatagcatct
    44221 gggtcaattc accatatact gctgttctta atggatctgt ctgcaactct ttctcaatat
    44281 ctgctaacaa aaatttttgc cgattgattt ttcgattctc tcctgttttt tctacgcctt
    44341 tgtaatcatt tcgacttccc agcacacttt gaatataaat tgccggcacg cctggtaacg
    44401 ttaccaaaaa tgaatgagca gctaaaaagc gtttgcttct ccattccgtc gtatctgttg
    44461 gagaacttaa cgcatctaga tacgtagcgt ttatctcata tggactggtc gttccatctg
    44521 ggtttttctt gtagtttact cgggcaccct ctttcttcag ctgttccact aattcaagga
    44581 tcgttttttc cggcagaata cctcgtaccg gattcaatcc gataccgtca tgggaggcta
    44641 aaaaattaaa aaaagttgtt tttttcgatg cgaaatcgat ggtttttaac cattctttca
    44701 acttacttgt attacccgaa cgaatcgctt gaagggtcaa aggtggtagc gaaaactgat
    44761 agaccatctg tgcttcattc tcgccattcc caaaataact gatattatct ttgtgtggca
    44821 catttgtctc agtgatcaat accgttcctg gagctgcttc atcagcgatt gcacggaata
    44881 attggatgat tttatgcgtt tctggcaggt gtatacaaga agttcctagt tctttccata
    44941 aaaatcccac agcgtccaag cgaataaatg aagcgccttt ttctaagtaa aacaaaagaa
    45001 tatcgatcat tttcaataaa acttctggat ttttaaaatt caagtctact tgatcttcgc
    45061 taaaagtcgt ccatacgtgt ttttctttcc cgtttttgtc aatgaacgtt gtaagtaacg
    45121 ggctggtcct tggtctagtc acgctgcttg tatctgtatc cagtggcaaa tcaataaaaa
    45181 aatctttgta cgtatcattc aagtttaaat attcctgaaa ccaagtactt tcggcagaca
    45241 tatgattgca gacaaagtcg aacattaacc gtgtttcgct tctcatttct tctatatctt
    45301 cccatgtgcc caaacggtcg ttgattgcct gataatttac aacggagaaa ccatcatcag
    45361 atgtatatgg gaaaaatggc aagaaatgta caactctaaa tgtatctgcc aaatactttt
    45421 gataaaattt cttaaatgta cgcaaagttg gttgctgctc ttcaaaaaat tgatccccat
    45481 aagtaatcaa gaaaatatct tcttcgtccc aatgcagttt gcctgcctgg cctttcttct
    45541 gctgataaac actgactttt tgttcaatca tagaggcggt tttctcagac aaatctgagt
    45601 cgcgataaat cgcttgtaaa agttttgtat acatcggatt cccctttctt ttttgggagc
    45661 gttcccaacg atttgatcat attatggaaa acgcttactg tcaatagcat ttttttaaat
    45721 aattttgata tacacaaaaa tacaaataag aaagcttatg tatcaataat taatagcttc
    45781 ttaaaaaaat atggtctctc atgatcatgc aagctattcc taaattttca aaatcataga
    45841 caaaaaagaa tgtctattgt actatggaat tattctaaaa cgctcccaaa aaggaggtgc
    45901 atataaaatt gaaacctact atttatgaca ttgcgaaaaa agctaatgtc tcaaaatcaa
    45961 ctgtctccag agtattaaat aaccagccaa atatctctga taaagcaaaa gcaagtgtac
    46021 ttcaagcaat caaagaattg aattatgaac cgagcaaaat tgcaagagga ctgtcaggaa
    46081 atggttttga cgcgattcta gcattatctc atcgtacttc tcatacgacc tatggcaatc
    46141 cttttttttc agatgttatt caatccattt ctgccgtttc tgaacaaaat aacttcgact
    46201 tgattctaca aacggcaaaa acaccagaag aagagctaga aaaatgcgcg aaaaagatcc
    46261 aagaaaaaat gatccgagga attattattt taagctctac gatggatgaa acacttcttt
    46321 ttgaattgga tcaatataat attcctattg tggtaatcgg aaaagtcgga aacgactaca
    46381 cgaacatcta ctcagtggac acggataatt tccaggatag ttacgatttg gtggaaacac
    46441 tgatcaagaa tggacaccgt catatcggct gtctccatgc tccgacagaa gtttatgtat
    46501 ccaatgatcg tctcaatggt taccgtcaat gcttatttga tcaccaccta gatatccgta
    46561 atgaatggat tatagacggc ggctttcgct tagaagatag catggcagca atgaatgatt
    46621 taatgaagct acgagaaaga ccaactgcga ttttttctac cgatgcatta aaatcattga
    46681 gtatttacaa agtggcagaa caatctggaa agcagatacc tgatgatctt tcgatcgcag
    46741 gattcagtga tcccttattg tcctccctat tttcgccaca gttgactcgg atagacattc
    46801 cgacaaatca gttaggaatc aaagccacag aacttttatt ccaactaatc caccaccaat
    46861 accctgaaga aaaaagaatc attctttcaa ccgagattac tctcacagat tcgatccaac
    46921 aacaagaaat gtagaacaga attataaaag aacagaaaca ctgaggataa gtcagttact
    46981 ctgttctttt ttcaatcact catccctcgt taccccctcc cggtatttta gcgaattttt
    47041 cttattttcc atctgtttta gatagctgtg gataactata atgtttgtgt taaactattt
    47101 tttccacagg gaagaaaaaa ctatttttaa ccacgttgtg tataagtttt tatagttcgt
    47161 ttttttaaac acatttaccc ttatttcaat ccatgttatt tggggatact tatctctaaa
    47221 acataaatag aataacagac atgtctactg gttttgtttt attcgaggaa ttattcaata
    47281 atagctagca aaatattaaa aataaccgct ctatcttccc atcaatttat tttgcattca
    47341 aaaaatgatc tatctttact tttgttgatt gacttctctt ggaagatgat cgttccgata
    47401 ccttaattgg atacgaaccg atgttgttaa gatcctttcc cacttttctt ctagttagac
    47461 aaattatttt agacaaacca aaaaagaggt tgtgacaata atttgtcaca acctcttttt
    47521 taaaatatct attttgtttg attgttcatt gcgtcttctg cagccatttg cgcttcaata
    47581 tctgaaatac tgtagacatt ttcgcttgct acaccgactt cttttggttc catgtcacga
    47641 taacgttgca taccagtacc agctgggatg atcttaccta taataacatt ttctttcaaa
    47701 ccaagaagag gatctttctt accgcggatc gcagcatccg tcaagacacg agttgtttct
    47761 tggaatgatg cagcagataa gaaactattt gtttctaatg atgcttttgt gatacctagt
    47821 aagacaggtc gagaagttgc aggaacgcct cctgagacta gcgtattgta gttttgatct
    47881 ttaaagtctg cgatgtctaa taatgtacct ggtaagatat ctgtatcacc tggatccatt
    47941 acacgaactt tacgcagcat ttgacgaacc attacttcga tatgcttgtc gccgatttct
    48001 accccttgca tacggtaaac acgttgtact tcacgaagca agtagttttc aacagataat
    48061 acatcgcgaa cttgcaacaa ttgttttgga tcgattgatc cttctgtcaa tggtgcacca
    48121 cggtggatca tatcgccttc tgctactttc atacgtgctg tataaggaac agtatacgta
    48181 cgtgtatctg tttttccttt gattgttact tctttggtac gtgttgcagg atcttctgag
    48241 atatcgatga catcccctgt tacttctgtg atcactgctt gccctttcgg attacgtgct
    48301 tcaaagattt cttggacacg aggaagacct tgagtgatat cgtctccggc aaccccacct
    48361 gtatggaatg tacgcatcgt caactgagta ccaggttcac cgattgattg agcggcgatc
    48421 gtaccaactg cttctccgac ttcaacgtca gagccagttg caaggttgcg tccgtaacaa
    48481 tgtttgcaga caccatgttt tgtattacat gtgaagacag aacggattgt cactgtttcg
    48541 acacctgcat caacgatttg ttttgcgatg tcttcggaaa tcaattggtt tcctgcaatg
    48601 atcaattcat ttgtttcagg atgacgtact tctttacggg tgtaacgtcc cactaaacga
    48661 tcttctaatg attcgatgat ttcatttccc tcgcggatgg cttggatttc aagacctcgg
    48721 tcagtaccgc aatcttcttc acggatgatt acatcttggg ccacgtcaac cagacgacga
    48781 gtcaagtaac ctgaatcggc agtcttcaag gctgtatcgg tcatcccttt acgagccccg
    48841 tgagttgaga taaacatttc caagacggat agtccttcac ggaagttcga aatgattggc
    48901 aattccatga ttcgaccatt aggagcggcc atcaaaccac gcattccggc aagctgtgta
    48961 aagttggaaa tgttaccacg agctccagaa tcagacatca tgaagattgg atttttcgct
    49021 tccaaacctt ccatcagttt ttgttggatg ctgtcttttg cagcattcca aacagcaatg
    49081 acacgttcat aacgttcgtc atctgtgatc agaccacgac ggaattgttt tgtaatagtt
    49141 tcaacttgtt tatgggcatt ttcaatgatt tcttgttttt cattcagtac actgatgtca
    49201 gcgatcccta ctgtgatacc ggcatgtgtc gaatgtttgt accctaagtc tttcatacgg
    49261 tcaagcatct tagaagtttc tgttactttg aatcgtttga agacttctgc gatgatgttt
    49321 cctaagtttt tcttcttgaa cggtaatact aattcttgct ctttgatatg tgcaggaata
    49381 tcagtaccag cttcaacaaa gtatttgtcc ggtgtttgga cggtcaagtt gaagtctgtt
    49441 ggttcattca gataagggaa ttcaggaggc atgatctcgt tgaagatgat cttaccaact
    49501 gttgtgatca tcgtacgttc tttttgccat tcagtaaatg gtttttcacc taatgagtta
    49561 gggttcacac cgatacgtga atgtaaatga acatatccgt tgcgccatgc gataacggct
    49621 tcgttttgat ctctgaagac cattccttcg ccttcgcgtc cttcttcttc cattgtcaag
    49681 tagtagttac ctagaaccat atcttgagaa ggagtaacaa ctggtttacc atctttaggg
    49741 ttcaagatgt tttgagcagc taacatcaac atacgtgctt ctgcttgtgc ttcttcattc
    49801 aatggaacgt ggacagccat ttggtctcca tcgaaatcgg cattataagc ttcacataca
    49861 agtggatgca aacggattgc gcgtccttgt actaaaactg gttcgaatgc ttggatacca
    49921 agtcggtgaa gcgtaggtgc ccggttcagc aatactgggt gttccttgat tacgtcttct
    49981 aaaacatccc atacttcgtc ttcttgacgt tcgattttac gtttcgcatt tttgatgttt
    50041 gaagctagtt cgcgttgaac aagctcacgc atcacgaatg gtttgaataa ttcaatggcc
    50101 atttcttttg gaagtccaca ttggtacatc ttcaagaaag gtccaacaac gataacggaa
    50161 cgacctgagt agtcgacacg tttacctagc aagttttgac ggaaacgacc ttgtttccct
    50221 ttcaacatat gagaaagaga tttcaatggg cggttacctg gtccagtaac tggacgtccg
    50281 cgacgaccgt tatcgatcaa tgcatctact gcttcttgca gcatacgttt ttcattttga
    50341 acgatgattc caggtgcatt caagtctaac aaacgtttca aacggttatt acggttgatt
    50401 acacgacggt aaagatcatt caagtcactt gttgcaaaac gtccaccttc taattgaacc
    50461 attgggcgta aatctggtgg aataacaggg ataacgtcca taaccatcca gcttggttga
    50521 tttcctgaag cgcggaatgc ttctaaaata tccaaacggc ggattgcacg tgtacgtttt
    50581 tgtccttgtg cagatttcaa ttcttctttc aattcggctg cttctgcatc tagatcaaca
    50641 ttatcaagta attgtttaat cgcttctgca cccatagcag cttggaattc ttgtccatat
    50701 tgttcacgtt tttcgcgata ttcgcgttct gtcaacaatt gttttttctc taaattcgta
    50761 tttcctggtt cgatgactac atatgaagca aaataaatga tttcttccaa agcgcgtggg
    50821 ctcatgtcta atacaagacc catacgtgaa ggaatccctt taaagtacca gatatgtgaa
    50881 acaggagctg ctaattcaat atgtcccatg cgttcacggc gaactttcga acgagtaact
    50941 tccactccgc atcgatcaca aacgattcct ttataacgga tacgtttgta ttttccgcag
    51001 gcacattccc agtcttttgt aggaccaaaa atccgctcac agaacaaccc ttcacgttca
    51061 ggttttaagg tacgatagtt aatcgtctca ggttttttta cttcaccgta agaccagctt
    51121 ctgatttttt cgggagaagc caaacctatt tgcatacttt cgaatttatt tacatcgatc
    51181 aaaaggggtt ccctccattt catttaatgg caaaccgcaa gacggctgat cttcgaccgc
    51241 cttgcagctc atagaccgga ttagtctaaa ttatcttctg cggtttcaaa gttttggatg
    51301 acatcgtctt tttcatcttc cacttgttca gcagctttct tttctaattc tttggcagtt
    51361 tgctgttcgg caaattttgt caaagcatca acagtgatca aatcgtcgtc ttcatcgtcc
    51421 atgtcgcgaa gttcgatctc tgtttcttcg atatccaaga cacgcatgtc cagacctagt
    51481 gattgcaatt ctttcaccaa tacgcggaat gattccggta cacctggttt tggaattggt
    51541 tcacccttga cgattgcttc gtatgttttc acacgtccga ctacgtcatc tgatttgtat
    51601 gtcaagattt cttgtaaagt ataagccgca ccgtaagctt ccagtgccca tacttccatt
    51661 tcaccaaaac gttgtccacc aaattgagct ttacctccca atggttgttg tgtaaccaat
    51721 gagtaaggtc cgattgaacg tgcatgcaat ttgtcatcga ccatgtgagc aagtttgatc
    51781 atgtacatca caccgacaga cacacgtcca tcaaatggtt cacctgtacg tccgtcgtac
    51841 agaacagttt tcgcatcgct ggccataccg gcttcacgaa ctgtttccca tacgtcttca
    51901 tcacttgcac catcaaatac tggcgttgct acgtggatac ctaactgacg tgcagccata
    51961 cccaagtgta attccaatac ttgcccgatg ttcatacgtg aaggcacccc taaaggattc
    52021 agcatgatat cgattggtgt tccatcaggt aggaatggca tatcttcttc cggcataata
    52081 cgggatacaa cccctttatt tccgtgacga ccggccattt tatccccttc atggatttta
    52141 cgtttttgaa cgatataaac acgaactaac atgtttacgc ctggtgacaa ttcatctcca
    52201 gcttcacgag taaagatttt cacatcgtga acgataccgc cgccgccatg tggtacacgt
    52261 aatgatgtat cgcggacttc acgagctttt tcgccaaaga tcgcatgcaa tagacgttct
    52321 tctgcagata attctgttac cccttttggc gtgactttcc ctacaagcaa atcgccatct
    52381 tggacttctg caccaatacg gatgataccc atttcgtcta aatcttttaa tgcgtcttcc
    52441 ccgacattag ggatttcgcg tgtgatttct tcaggtccta attttgtatc gcgggcttct
    52501 gattcatatt cttcaatatg gatagaagtg tatacgtcat cttttaccaa acgacggctc
    52561 atgatgatcg catcttcata gttgtaccct tcccaagtca tgaaaccaac taaaacgttt
    52621 tggcctagag ccatttcgcc ttgttccata gatggtccgt ctgccagagt atcccctttt
    52681 tcgactttct cacccaaatg aacgattgga cgttggttgt agcttgttcc ggagtttgaa
    52741 cgacggaatt ttgttacatc gtatttatct aatgcaccgt tgtcgcggcg tacgcggatt
    52801 tcagaagcat caacgtattc aacgacaccg tcatgtttac ataacaaagc agctcctgag
    52861 tcatgcgctg atttgtattc cataccagta ccaacccatg gtgcttgagg attgatcaac
    52921 ggcactgctt gacgctgcat gttggcaccc atcaaggcac ggttggagtc atcgttttcc
    52981 aagaaaggaa tacatgctgt cgcaacggca actacttgtt taggtgatac gtccatgtag
    53041 tctactttat caatagatac ttctaagttt tcactttgag cacgagccat aacaacgtct
    53101 tgagcaaatg ttccatcttc attcaatggg ctgtttgctt gggctacgat gtaatggtct
    53161 tcgatatcag ctgtcaagta gtcgatttgg tcagtaacac gacctgtttc acggtctaca
    53221 cgacgataag gtgtttcgat aaatccgaat ttatttactt tcgcataact ggacaaacta
    53281 ttgatcaacc cgatgtttgg gccttcagga gtttcgatcg gacacatacg gccatagtgg
    53341 gagtagtgaa cgtcacgaac ttcataaccg gcgcggtcac gagtcaatcc gccaggtcct
    53401 aaggctgaaa gacgacgttt gtgtgtcaat tcgccaagag ggtttgtttg atccatgaac
    53461 tgtgacaact gagaagaacc aaagaattct ttgatacttg ccacaactgg acggatattg
    53521 atcaattgtt gaggtgtcaa tgtttcagta tcttggatcg acattctttc acgaaccaca
    53581 cgttccatac gtgctagccc gatacggaat tggttttgta aaagttcacc gactgaacgg
    53641 atacgacgat ttcctaagtg gtcgatatca tcgacattgc cgataccttc catcaagttc
    53701 aagaaatagc tcattgaagc aatgatgtcg gctggacgaa ccgtttttac agctgcttct
    53761 ggatagccgt ttccgatcac gtttacttca cgttcagggt ctcttggtga aaatactttg
    53821 atgacttgga cagtcattgg atctgtgacc acaccgtctt ctgatggata gtaagtcact
    53881 gaatttaggc cattatcaat gaaaggagct agtgtttcca tgacttggtg agtcagaact
    53941 gttccttttt caacgatgat ctcgcctgtt tctggatcaa ctaatgtttc agctaatgtc
    54001 agattcaata gacgtgtttt caaatctaat tttttgttta ctttgtaacg accaacgttt
    54061 gctaagtcgt aacgttttgg atcgaagaaa cgagcattca gcaagttacg tgagctgtct
    54121 gctgtttttg gttcgcctgg acgcaaacgt tcgtaaacgt ctttcaaacc ttcttctgtt
    54181 cttgagtcgc ttgcgttttt atgcaagtct ttttcgactg tattgcgtaa tgtttcgctg
    54241 tcgccgaaga tttcaaagat cgtatcatcg gaaccaaatc ctaacgcacg aactaatacg
    54301 gttaaaggaa tcttacgtgt acggtcgata cgtacataag agatgtcttt tgcatcagtt
    54361 tccatttcta gccaagctcc acggttaggg ataacagtag aaccgaatcc ttcttttccg
    54421 tttttgtcga cttttccatg gaaatagaca cctggtgaac ggactaactg agaaacgatt
    54481 actcgctctg ccccgttgat gatgaatgtt ccttgttctg tcattaatgg gaaatcaccg
    54541 aagaatactt cttgtgcttt gatttcgcca gtctcgcggt ttgtcaaacg caatgttaca
    54601 tgtaatggtg cagagtagtt tgcatcatgc gcacgagctt ctgctactgt atactttggt
    54661 tctttcaatt cataatctac aaattccaaa gatagattgc cattgaagtc atcgattggc
    54721 aaaatgtctt caaacatttc tctaagtccc tcatctaaga accattggta agagtctgtt
    54781 tgaatttcga tcaaatttgg taattccaac acttcactga ttcttgcgaa acttctacgt
    54841 tcgcgatgtt ttccgtattt tactacgtgt ccagccaagc tcttcacccc tctaaaatgt
    54901 cattcaaaaa gactacccga tcaattgtta caataatatt tcaaatatta tttttaggta
    54961 acaacagaca ttttttgcgg cttttaggca aaaaaaaacc aaaaatagaa cattctcttg
    55021 aaaccttact tcgtgagctt ctacttttgt ttccttatat taaagccgaa acggacaata
    55081 tttcgtttct gccttttctt catcagatag tttttgcaac ttcatatact acacttattt
    55141 tctataaaag tcaacacttc ctggcgtttc ttctgctttt tcgcaacatt ttcttaatgt
    55201 tttcaatttt atgttttcaa ttatctgaca attcttaatt gacagacctt tttatctcgg
    55261 ttatacttta gaaaattcat tttgtggagg aacttctatg cgaaccaaca tcaaccaaat
    55321 cgcaccaaca caactgaata acttttactt tagctacttt agataggttt gtattcatca
    55381 tgatggatac aaacgttaaa cgacagtttg tatctatgat ggctaaagaa ctttgtattg
    55441 cagcaaaaag ccacgtagac aaaatgctaa gtggctgagc agtgaaaata caaagtaaaa
    55501 gtatcaagca ttctactttt tcaatttatt ttccgacttt tcaatccatt tggacatttt
    55561 gtctgaatgg attttttgtt tcccaaaatt aaggaggaaa aagaaatgaa aaaaaatata
    55621 atcatcggat taggcgctgc cctgctacta gctttaggag gttgcgcaca aaaagacaat
    55681 tccgcagagg catcagatgg acaaaaaaca attggcgtat tgcaacttgt acaacataac
    55741 tcgttagatg ctgcctataa aggattcaaa gaaggattag cagaagaagg gtataaagaa
    55801 ggcgataatc taacaatcga ttatcaaaat gcccaaaaca atcaagacaa cctgaaaagt
    55861 atgagtgaga aactcgtcaa agaagatcct gatttgctac ttggaatcgc cactccagca
    55921 gcacaatctt tattaaatga aacaaccact atcccgatga ctgtcacagc ggtcacagac
    55981 ttggaacaag cccatttagt cacatccaat gaaaaacctg gcggcaatgt gactggcacg
    56041 acagatatgg tgccaatcga aaaacaaatc ggcttactgt taaatatcgt gccagacgcc
    56101 aaaacaatcg gcatcatgta taacaacggt gaagcaaact ctaagatcca agctgatttg
    56161 gcagaaaaag aattgaaaaa agcgggcgtc aaagtcaaag tactgacagc aaattccaca
    56221 aatgatgtcc aacaagtgac gaaaagtcta gctaaagatg tagatggaat ctatattcct
    56281 actgataaca cgttcgcaag tgcggcaagt gttgtcggtg aagtcgcaaa agaaacaaaa
    56341 acacctgtcg ttgctggcgc agttgatcaa gtcaaagacg gcggtctagc aacagtagga
    56401 atcgactacg aaagcttggg caaacaaaca gggatcatgg cagcaaaaat cttagacggc
    56461 aagaatccaa gtgaacttcc tgtcgaaaca gcgaatgatt tggaactgta tgtcaataaa
    56521 gacatggcca aagcattagg catcaatcca gacagcatca aagaagctga ataaaacaca
    56581 ggaggattct catgacaaat ttgctctctc ttttcttatc atctgcatcg caaggggtct
    56641 tatgggccct acttgcgatt ggtgtatacg tcactttccg tatcttggac attgccgact
    56701 tgactgctga aggaagcttt cctttaggtg cagggatcgc ggcagtaagt attacaaatg
    56761 gctatcatcc attgacagct tgtcttctag gctttttagg gggtgcggca gcagggcttg
    56821 tttccggact gcttcacaca aaattgaaaa ttcccgccct tcttgcaggg atcatcacca
    56881 tgactggttt atattctgtc acatcccgtg tgatgggtgc gccgaacatc tctttattgg
    56941 gtcagccaac gatctttcat tgggtcgagt cattcggcat aacaaaagac aacgctgtac
    57001 tgatcgtcgg tctgcttttt gctttgatcg ttgtatgttt acttgttttg ttcttacgta
    57061 cagaaacagg acttgctatt cgtgcaactg gcgataatct ggcaatgagc gaagcaaacg
    57121 ggatcaacac agataatatg aagatcatcg gttatatgat ttctaatgga tgcattgctc
    57181 tttctggctc cttacttgcg caaaacaacg gctttgctga tttaaattct ggtattggaa
    57241 cgatcgtaat tggacttgct tctattatca tcgcagaagt tttgttacgt aaccagccac
    57301 tatttctccg tctcttgact gtcgttttag gtgcggtcat ttatcgtttt attttagcgt
    57361 tagtcttcga actgaatgtt gaaccaagtg attcgaaact tgcttctgct ttcgtgctag
    57421 tgatctgtct ttctttcccg cagatccagc aaaaattcca tttacctcaa aaaaagaaag
    57481 gcggtagtgt caaatgaatg acgtcttaac attggaaaat ctccatcata cctttgaaaa
    57541 aggaacgatc aacgaaaatc atgtcctgaa aggcattaat ctttccttag atcccggcga
    57601 cttcgtgacg atcatcggtg gaaatggggc tggtaaatct actttgctga acagcatcgc
    57661 cggtacattt tctgtcgatc agggtcaaat tttattgaat ggaaaaaata tcacgaaaaa
    57721 atctgttgtc gaacgttcga aatccatcag tcgcgtattc caagatccta aactagggac
    57781 agccgttcgt ttaacggtag aagaaaatct tgctcttgct atgaaacgag gaaaaaaacg
    57841 cggcttcttc cgtggtgtca aaccacaaga ccgaacattt ttcaaagacc agctggttcg
    57901 gctaaatttg gggttggaaa atcgtttatc ctcagaaatc ggtttgctgt ctggaggaca
    57961 acgtcaagct attacgttat tgatggccac attacagcgt ccagaactga tcttattaga
    58021 tgaacacact gcagcgcttg accctgctac ttccaaaacg gtgatggaat tgacagaaaa
    58081 gctgatcact gagcagaatc tcactgcttt tatggtcact catgacatgg aagacgcgat
    58141 ccgctatggg agtcgactga tcatgctgca tcaaggaagg atcgtcgtag atatccagca
    58201 agaggaaaaa cagcagctca cagtgcccca attattagcg ttgttcaaac aaaacagcgg
    58261 cacggcatta aaagacgatc aagtactttt atcttcataa tcagaaagag gatgggacaa
    58321 aaaatgaacg cccaccctct tcttttttct ctttccttct ttcgtttttg ctttatgtat
    58381 caaccgttgt ttcttcgaaa actattttgt tctctccttc ttcctgatag aatggatggg
    58441 aaaggagctt gatgccatga caacgaaaga caaggtactt caattattga aaaagacacc
    58501 agattttctt tccggagaaa agttggcaca gcaactggaa gtttcccgga caagtatttg
    58561 gaaagccatc aaagaattgg aaaaggcagg ctatcgtttt gagcacgggc ctacagggta
    58621 tcgttacctg ccctctgacg tactagatgc aaaagaaatc cagcaagagc tacggtcgtt
    58681 tattcctgat ttgaaagtac aaacgatgtt gacttctgaa tctacgatga aagatgccaa
    58741 actggcagca gctcaaggag attctgtttc gaagttattt gttgctgata cacaagaaca
    58801 agctcgtgga cgttttggac ggcctttttt cgctgaacct ggacgaggaa tctacatgag
    58861 tctcttgctc catccagaca aacggtttga tgaattgcct caatatacga tcatcgcagc
    58921 cagtgcctgt gtgaaagcca ttgaatctgt aaccggtcgt tctgcttcga tcaaatgggt
    58981 caatgatatc tacatggatg gaaaaaaagt ctgtgggatt ttatctgaag cagtcagtga
    59041 tatggaaagc ggcaggatca gtcatatcat cattgggatc gggcttaatt tttctgttcc
    59101 tcaaaatcat tttccagctg atctccaaga aaaagccatc tctgtctttc ccgacggaca
    59161 agctagtgtc acaaggaatc agctaatcag caaaatctgg caagaattgt tcacttatct
    59221 ttctcaccca gaaaaagcgt tggacttcta taaagaaaaa tcattcgtat tgggaaaaac
    59281 cgtgtcgttc aatcaaaaag gaacgactta tgtcggtctt gcaaaagaaa tcggtgatca
    59341 tggcgaactg atcgtccaaa caaatgaaca gaagatgagt ttgtcttctg gcgaaatcag
    59401 tctcacatct atccgtacgc aataattatc agatttcaca aaaggacacc tgatctcata
    59461 cagtgtcctt ttttctgaat tctcttcaaa agactctcct tattctatca gctgacagtt
    59521 tctctatcct ctagaaaaag gtattctaaa aatatctata aagcatggta cactttcttt
    59581 aaatgaatca atgcaaaaaa caagagagtc atcagtttac acgatcaaaa gagatagcag
    59641 gccgcttcct ttttcaatct tatagataga ccatgggtaa cgataaaaac aaaaaagaac
    59701 ttgcgactta ttttgccgta agttcttttt tagctagtgc cttaatctct taatcccaac
    59761 aaacgtctcg catagatcac ctgaccagag tgcatagcag catcatcaat aatagaaacg
    59821 agacgcattc ctcgtgtgac tggtggtgtc cagttctcgt caattacttc atctagctcg
    59881 gaaggttcca gccttcttaa gtaagaaata gcaaaatcag cagctcctcg caagtatcct
    59941 agcagatctt ctatttgatt agcttggatc tgctttgctt gttccagact atgcttccaa
    60001 tcttgttcgt tatctgctat cgtcatcgga aaccggcttt cccagtcttg actatgccaa
    60061 attggagaag ttcctgctaa atcagcaatc tggaaatcta attctcgagc ggtatgccaa
    60121 gccagccaag tcattgattt gatctgcggg gctctatctt ccactggaaa acgattggcc
    60181 tcctcaatag agacgccttc aaacaaacgt tcaaaccgat cgtatgcccg atccacactc
    60241 tcgcatagca tatctaaata atccatttat tttcctcctt tttatttatc ctacttactt
    60301 tttcctaatt ctaggatacc tacttatatg ttactacaca cccttatttt ttacacttta
    60361 acagtcacca tcatcaatag tttggagatt gaaagaaaaa gatagtgtcg aactatcaat
    60421 tgaacaagaa gcaacaatat gaacgtttca ttaatattct ttaaataaac aatgattttt
    60481 caaacgaaca gtttaagact aggagaaaaa gagtataata ggaaaatgtt tgaaaggaaa
    60541 ggaaggggaa aaatgaaaaa aagggcaagt ttactagtcc tagttgcttt gttgttcaat
    60601 atcttttcgt atggtgtggc cgtttcagcg gaaacataca ttcctaaaga ccctgggaat
    60661 atttataatg atctaactgg taactatggc ttgttaggaa ttgctagtca attccacgta
    60721 tttgctaaaa ataagacaac tgtgaatgca catacagacg gaaatatagc aacacatgaa
    60781 cttgatgcaa aaaataattt tggtacagag cttcatgaag gtttattgaa ccaagaaata
    60841 aattatatac aaaaaactga ttctttcgct aattcttcag gcatacctac aggtagcaca
    60901 agaatgaata aatttgtcgt tggcagtaac gtggatgtag cacttcaaga tggtcatcct
    60961 acagtcaatg gatccaggct tgatcactta actccagatg atttttataa agatcgtgct
    61021 gggcatgaat atattgattt cgagcaggaa tttgctaaat tgaatgtagc agcaaatgat
    61081 cttgcaacga tcactccagc taaaacatat actgctgcag attttccaga catgaacaat
    61141 cggacaatcg atttaactga tttatcagat accgggtttt tattggtaaa tatcgatgca
    61201 gaagtgttaa cgatgaatac gccacttcaa atcattaatc caaatgatca agtagttgta
    61261 tttaatgtca tcaacagttc ttctgcattg aacgtacagt caccaatcaa atacaatgat
    61321 cgttcaaatc atgaaacaga agatttttcg gatgcgaata tctcttggaa ttttggaaat
    61381 gagatgactg atttgacaat cagtgctcct tttcaaggaa caatccttgc gcctaatgct
    61441 actatccgca ttactcaaaa ccaagacgga acgattatag gaacaaatgt gattttagat
    61501 gcagctacca atcgttggga tcctaacgaa atatttatca gcgacaatgg tacagatacg
    61561 acagacacaa gcgacagtac agatacatct accacaagtg atagctcaga ttcatcaact
    61621 tcaagcgaca gtacagatac tacctctacc acaagtgata gcacggatac atcagcttca
    61681 agcgacag
//
LOCUS       DACDXE010000007        57916 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.007, whole genome shotgun sequence.
ACCESSION   DACDXE010000007 DACDXE010000000
VERSION     DACDXE010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.007"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(279..833)
                     /locus_tag="IWM23_000422"
     CDS             complement(279..833)
                     /locus_tag="IWM23_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAP9883095.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(959..2887)
                     /locus_tag="IWM23_000423"
     CDS             complement(959..2887)
                     /locus_tag="IWM23_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAP9883096.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(3193..3909)
                     /locus_tag="IWM23_000424"
     CDS             complement(3193..3909)
                     /locus_tag="IWM23_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9883097.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(3910..5733)
                     /gene="glpO"
                     /locus_tag="IWM23_000425"
     CDS             complement(3910..5733)
                     /gene="glpO"
                     /locus_tag="IWM23_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAP9883098.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(5747..7243)
                     /gene="glpK"
                     /locus_tag="IWM23_000426"
     CDS             complement(5747..7243)
                     /gene="glpK"
                     /locus_tag="IWM23_000426"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAP9883099.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(7379..8839)
                     /locus_tag="IWM23_000427"
     CDS             complement(7379..8839)
                     /locus_tag="IWM23_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9883100.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(8829..10124)
                     /locus_tag="IWM23_000428"
     CDS             complement(8829..10124)
                     /locus_tag="IWM23_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9883101.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(10456..11133)
                     /locus_tag="IWM23_000429"
     CDS             complement(10456..11133)
                     /locus_tag="IWM23_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAP9883102.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(11163..12254)
                     /locus_tag="IWM23_000430"
     CDS             complement(11163..12254)
                     /locus_tag="IWM23_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAP9883103.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(12355..12789)
                     /locus_tag="IWM23_000431"
     CDS             complement(12355..12789)
                     /locus_tag="IWM23_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9883104.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(13373..14830)
                     /gene="ascB"
                     /locus_tag="IWM23_000432"
     CDS             complement(13373..14830)
                     /gene="ascB"
                     /locus_tag="IWM23_000432"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAP9883105.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(14827..16722)
                     /locus_tag="IWM23_000433"
     CDS             complement(14827..16722)
                     /locus_tag="IWM23_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9883106.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(16780..18555)
                     /locus_tag="IWM23_000434"
     CDS             complement(16780..18555)
                     /locus_tag="IWM23_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9883107.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(18605..20101)
                     /locus_tag="IWM23_000435"
     CDS             complement(18605..20101)
                     /locus_tag="IWM23_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9883108.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(20295..21917)
                     /locus_tag="IWM23_000436"
     CDS             complement(20295..21917)
                     /locus_tag="IWM23_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9883109.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(22366..23679)
                     /locus_tag="IWM23_000437"
     CDS             complement(22366..23679)
                     /locus_tag="IWM23_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9883110.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(23924..24148)
                     /locus_tag="IWM23_000438"
     CDS             complement(23924..24148)
                     /locus_tag="IWM23_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883111.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(24168..24536)
                     /locus_tag="IWM23_000439"
     CDS             complement(24168..24536)
                     /locus_tag="IWM23_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9883112.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(24734..25444)
                     /locus_tag="IWM23_000440"
     CDS             complement(24734..25444)
                     /locus_tag="IWM23_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883113.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(25462..25959)
                     /locus_tag="IWM23_000441"
     CDS             complement(25462..25959)
                     /locus_tag="IWM23_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883114.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(25996..26448)
                     /locus_tag="IWM23_000442"
     CDS             complement(25996..26448)
                     /locus_tag="IWM23_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9883115.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(26583..27188)
                     /locus_tag="IWM23_000443"
     CDS             complement(26583..27188)
                     /locus_tag="IWM23_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9883116.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(27638..28258)
                     /locus_tag="IWM23_000444"
     CDS             complement(27638..28258)
                     /locus_tag="IWM23_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9883117.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(28260..29129)
                     /locus_tag="IWM23_000445"
     CDS             complement(28260..29129)
                     /locus_tag="IWM23_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAP9883118.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(29152..29559)
                     /locus_tag="IWM23_000446"
     CDS             complement(29152..29559)
                     /locus_tag="IWM23_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9883119.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(29578..30867)
                     /locus_tag="IWM23_000447"
     CDS             complement(29578..30867)
                     /locus_tag="IWM23_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883120.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(30894..31196)
                     /locus_tag="IWM23_000448"
     CDS             complement(30894..31196)
                     /locus_tag="IWM23_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883121.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(31221..33314)
                     /locus_tag="IWM23_000449"
     CDS             complement(31221..33314)
                     /locus_tag="IWM23_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9883122.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(33803..34567)
                     /locus_tag="IWM23_000450"
     CDS             complement(33803..34567)
                     /locus_tag="IWM23_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAP9883123.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(34678..35547)
                     /gene="mdcB"
                     /locus_tag="IWM23_000451"
     CDS             complement(34678..35547)
                     /gene="mdcB"
                     /locus_tag="IWM23_000451"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAP9883124.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(35572..36648)
                     /locus_tag="IWM23_000452"
     CDS             complement(35572..36648)
                     /locus_tag="IWM23_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAP9883125.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(36672..37265)
                     /locus_tag="IWM23_000453"
     CDS             complement(36672..37265)
                     /locus_tag="IWM23_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAP9883126.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(37269..38945)
                     /gene="mdcD"
                     /locus_tag="IWM23_000454"
     CDS             complement(37269..38945)
                     /gene="mdcD"
                     /locus_tag="IWM23_000454"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAP9883127.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(38935..39234)
                     /locus_tag="IWM23_000455"
     CDS             complement(38935..39234)
                     /locus_tag="IWM23_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAP9883128.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(39336..40982)
                     /gene="mdcA"
                     /locus_tag="IWM23_000456"
     CDS             complement(39336..40982)
                     /gene="mdcA"
                     /locus_tag="IWM23_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAP9883129.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(40989..41900)
                     /locus_tag="IWM23_000457"
     CDS             complement(40989..41900)
                     /locus_tag="IWM23_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9883130.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            42020..42877
                     /locus_tag="IWM23_000458"
     CDS             42020..42877
                     /locus_tag="IWM23_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9883131.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(43064..43426)
                     /locus_tag="IWM23_000459"
     CDS             complement(43064..43426)
                     /locus_tag="IWM23_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883132.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            43772..45211
                     /locus_tag="IWM23_000460"
     CDS             43772..45211
                     /locus_tag="IWM23_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9883133.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(45218..47770)
                     /locus_tag="IWM23_000461"
     CDS             complement(45218..47770)
                     /locus_tag="IWM23_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAP9883134.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(47884..49215)
                     /locus_tag="IWM23_000462"
     CDS             complement(47884..49215)
                     /locus_tag="IWM23_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883135.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(49181..50659)
                     /locus_tag="IWM23_000463"
     CDS             complement(49181..50659)
                     /locus_tag="IWM23_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883136.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(50964..52799)
                     /gene="tdc"
                     /locus_tag="IWM23_000464"
     CDS             complement(50964..52799)
                     /gene="tdc"
                     /locus_tag="IWM23_000464"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9883137.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            53269..54696
                     /locus_tag="IWM23_000465"
     CDS             53269..54696
                     /locus_tag="IWM23_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9883138.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(54788..55153)
                     /locus_tag="IWM23_000466"
     CDS             complement(54788..55153)
                     /locus_tag="IWM23_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9883139.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(55150..55515)
                     /locus_tag="IWM23_000467"
     CDS             complement(55150..55515)
                     /locus_tag="IWM23_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAP9883140.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(55707..55955)
                     /locus_tag="IWM23_000468"
     CDS             complement(55707..55955)
                     /locus_tag="IWM23_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883141.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            55954..56082
                     /locus_tag="IWM23_000469"
     CDS             55954..56082
                     /locus_tag="IWM23_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAP9883142.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            56139..57833
                     /locus_tag="IWM23_000470"
     CDS             56139..57833
                     /locus_tag="IWM23_000470"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAP9883143.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
BASE COUNT        16849 a        12340 c         9738 g        18989 t
ORIGIN      
        1 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
       61 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
      121 accaagaggt tgtgagccta ccatttaact ccgaatcaca agaaataatt tcaaaaacaa
      181 cttctcctgt ttttggtttg gttggcttat tatcgagaag tagctactgg gagtctattt
      241 atcaaaaaaa ggaatgcgac ataggtcaca cccctttttt attttctttg aaacatcttt
      301 cggtactcga cagcatacaa gacaaaagat tgtaaaagac cgtaagtgat gacggcaaaa
      361 atgactctca cccacagcgt tggtaattct gggaaaagca gcaaaaggat catgccaaca
      421 tagatgacag cagtcgctac tttgccatac cacttcgctc cacttaattt gatattttta
      481 gatagcccga ttaatccagc tacaaacata aagccttctt ttatcaagaa tagaccaaac
      541 aacagccaaa gccacggtcg ataccatgcc atagacaaaa tcagaaaaag ttgtgtcaat
      601 ttatctgcaa acggatcaag caccttccct aagtctgtta cctgattata tctccgagca
      661 atgaccccgt ctagataatc agacaaacct gagacaacaa tgaccgctcc tgctatataa
      721 aatgactgtc tcagcacaaa ataaagataa atcggcagca aaaaaatacg gaatatcgat
      781 aaaagattcg gaatcgtttg tatttcttta cgccagtctg tgcttaattt cattttatcg
      841 ccctacctcc tcagcactct tttcttttta ttttagctta cacggggaat aaaaccaatg
      901 atttcactgt ttttattttt ctttacagcc actgatatta tgagctaatt tgagataatc
      961 acttgctgtt ttcatcgttt actcttcttt cttcctgtcg tttgcgcatt tcttttacta
     1021 gtactttatg agcttttttt gatcctcgct ctaggtcacg ctgccgttta cgtgttttat
     1081 aatcgataaa cagactgtct aaatcgtaac ctgtcgggta cagatcttct gctgaacgca
     1141 ccagacgaac acgctgacga ggcacgtgga ttattttttt gtctaaatat acatcaatcg
     1201 tatcttcacc tatatcttca taaactaacg cttctttttg gtattcattg acaaatatcc
     1261 gatcaccttt tgcaaagagc tggcgttctt ttttattctc cttcctattt gtaactgctg
     1321 tcttaggaag aaattctttt ttctgtgtcc gataggcttt ttccgccaag taacgctctg
     1381 cttgatggat caatttcatc gacatatcca ttttatgagc aatccacaaa gcttggcttt
     1441 ctcctgtttc tccaacccgc aactgatatt taggacgaag tgcttcccga tcgaaagcca
     1501 tagcagccgg cacaaaatct tcatgatctc cggcgaattt cttgatttct ccataatggg
     1561 tcgtggcaat gatcaatgcc ccttgctcat acatagattc catgattgcg atcgctagtg
     1621 cagccccttc atttggctct gttccactgc caatctcatc taaaagtacc aacgtattcc
     1681 tttttatctt tttcaaaata gcagcgatgt tttgcatatg accagaaaat gtactcaacg
     1741 cattttctaa attttggtgg tcaccgacat cgacgaaaat ctcatcaaag acagcaagct
     1801 ctgtcccttc ttttgccgga acttgcatac cgaacatcgc catcaaagtc aataaaccga
     1861 ctgtcttcaa cacgactgtt ttgcctccgg catttgctcc ggtgataatc aagcctcgat
     1921 agtctttacc gatttcaaca tccaatggta ccgcatgctc aggtaaaaat ggatgtctgc
     1981 cttgcttgat aatgatatgt tccgatttat tgattctagg tgtgcttcca ttgatttctc
     2041 ggctaaattt tgcccgagca aagatgatat ctaacaccgt gatcgtttcg atcaataaat
     2101 cgatcacttc ttcgttttct gcgatagcac cagtcaatgc cgccaggact tgatattctt
     2161 cactgatttc ttcagctttt aacaactgat agtgttcatt caatttagaa atcacagttg
     2221 gttcaataaa aaccgtcgtt cttttatttg attcatcgat aatcgttcca tctattttat
     2281 ttttatagct ggctttgata ggaatcgtat agtattcgcc tttttgaaca atcattgctt
     2341 cttggatcat ttctttgttt ttcggatgac gcaagaattt cagtaatttt gactggattt
     2401 ctttttcgat tagttgtaat tgtttgcgta ctttacgcaa attacgtgac gcgtcgtcac
     2461 tgactttatt attttttatt ttctggtcaa tcaactcttc tacatttatc aaatcaggaa
     2521 gatgtttgct ataagcaaac aacaagggtg tctggtattg attcttatca aaaaactttg
     2581 tgatcatccg actgcttcta agaaaatccg catattcgat caaatcggtt ggctgcagaa
     2641 tcaacccttt tttgacttgt tctgtcagtg catcgatcct cggcaacccc ataaatggaa
     2701 catgctgact gctttctaag atcaagcgtg cttctcttgt ttcttcttga cgatccaaga
     2761 cagtttgtag gtttgtcgag accgtcatat cgctgattcg ttttttgcta tagtttccaa
     2821 ttgctcttgc ttggatttcc tttttgatcc gatcaaattg taattgacta atcatttcgt
     2881 tgtccatatt ctctcctttt cttgaagaga tacctgtccc aaaataaata agggcctaga
     2941 gataaataaa aggatagaac acagcttaaa aaagtcatgt cctatcctta ttagaataca
     3001 aagatattgt tttcctgaca ggtaccgttt cataagcaca ccaaataaac tttgtttatt
     3061 ggctattcga cttactgatt agataccagc tacactcaca aaacataccc cattcttcta
     3121 ggtcttattt attttccact taactatagc aagacacatt ttggttgtca aactttagta
     3181 tgttttatag atttacacca ttaatgaata gacacctacc gcgataattg ctccgatcat
     3241 tggacctgcg attggtaccc atgaatatgc ccaatctgaa gttcctttat tagctattgg
     3301 caataccgca tgtgctaatc gaggaccaaa gtcccgagca gggttgattg catatcctgt
     3361 tggccctcct aatgaaagac cgatggctaa gatcaatcca cctacagcga atacgttcgt
     3421 tccaggtgca aattcgtttt gtccaaaagc tagcaaccca agaactaata caaatgtacc
     3481 aattaattct gtgatcacat ttgctggata attccgtata gcaggacctg ttgcaaacgt
     3541 tcctaaaatc gcgctttcat ctttcgttgc attccaatgt ggcaaataag aaagccaaac
     3601 aagaatggcc cctgcaaaag ctcctaaaac ttgcgcaatg atgaaaggaa cgacaagatt
     3661 ccaactaatt gcccctgtca tcgccatagc tagagagact gcaggattca aatgagctgg
     3721 gcccatgaat ccagaaatat agacagccat tgttacagcg agtccccaac caaatgcgat
     3781 gacaatccag ccagaagcct gtgctttact tttgtttaaa ttcacagccg cacacacacc
     3841 atctccaagc aaaactagga tcagtgtccc aataaactcc cccatgattt gagtcatatc
     3901 actgttcatt tatttctccc cctttagttc ccgcagatct gattctgcaa tcaatgcttc
     3961 taaacgttta gtctgttgcg ccttctcctc ttctgtccat tcaaaatatc cagcaattgc
     4021 atcgatcaca ggttgcttga tctcatcaag ctgatctcgt tcaaacaaca gatgatttgt
     4081 acgacgaatg agataatcgc ctggggccaa aaccatttct tcttctaaac cataacgtaa
     4141 ccgagctgac tcagcgagac tcaaaccagg ataaggtgcc atttcttttg ctaattcaaa
     4201 gatacgtcgg gcattagtcc cgtaaaaatc agcgatataa gtagcatctt cttcctctaa
     4261 accggctgct actccttctt ttgctaattc tgtcactgtt tcttctaatt tagttggatc
     4321 gaagtttcca cctgaaatct gatatttttt agagtcgatt tctttcgtct ctattccgta
     4381 ttcttctttt aataattggc gtatcaatcg caaagctcct tctgccatct tacggtaatc
     4441 ggtgattttc ccaccagata aagtcagcaa accatctggt tctctttcta aagaactacc
     4501 tctggaaatc gtagaaggtg ctttttcatc acggctgttt tccaaatggt tcaacacgtc
     4561 ttctacttca gcacgagaaa ctttgttttc cttatattca cttactgtat cgacaacttt
     4621 attgaagctc ttgtctgaaa tcgatccatt atctccacca ttgtaatcag aaccagaatt
     4681 accaatcaac agtggacgaa gtcctgccca gcttgcttcg atatcattca atgtgatatt
     4741 tgcttctgga tagcgatgat tgatcacatc caatagataa tctacatctt cttgtgtgac
     4801 tttcggatcc gtaaaatctc cttggtaatc cgtatctgtc gtaccaaagt aagtcttgtt
     4861 ttctcttgga atagcaaaaa ccatccgccc atcttgcttt cctgtatcga agtatgtggg
     4921 ttgcggtaca ggcagttttt tcgcatctac aactaaatgg atccctttag ttggacgcat
     4981 tttaggagag actggacgcg taaaattcaa gtttcgtact ttatctaccc aaggaccact
     5041 cgtattgatc actaattttg ctttgatttc gatcacttca tctgtcagca ggtcacgagc
     5101 tttgacgcca acgatttgat cgccatcata taaaaagcca accgctttca ttttacttac
     5161 tagataagcc ccatcttcgg cagccttttt gatattatca atcactaaac gggcatcatt
     5221 gttgcggaaa tctagataga cacccgcgcc ttttagccct tcttttttca aaaacggttc
     5281 tctttccaat acttcttctg gtgtcagcgt ataattctca tatttggttc ctgtcacatt
     5341 tgccagtttg tcataaaggt ccattgctac tttgaccgag aacatattga aggttgttgc
     5401 tccttcgccc tcatagattg gcaaaagcat tggatctggt tttgggatgt gtggggcaat
     5461 actttgtacg actgcacgtt caccaactgt gtcagctact acttccacat caaatgtctt
     5521 cagataacga ataccgccat gaacaagttt ggtcgagcga gaggaagtcc cttctgcaaa
     5581 atcctgcatt tcgatcaatc ctgttttgat tcctgatgct gctgactgga ctgccacacc
     5641 ggcaccagtg attccgccac cgatgatcaa cagatccaat tcttcttgct gcattttttg
     5701 aatgctatct tgtcttgtct tgtttgaaaa catatggatt tcctccttat tcaaatgctt
     5761 gggtagcttt tactgctttt ttccaaccgt tgtaaagttt ttctcggcgc tcttcctcca
     5821 tttgaacttc aaatagcttg ccagcttcat aaaattcgcg gatctcgtct gtatctttcc
     5881 aataaccgac agctaatcct gctaaaaatg ctgcccctag cgccgttgtt tccaagtttt
     5941 ctgctcgttg gatcggtaca ttcaagatat ctgtttggaa ttgcatcaga tattcattgt
     6001 ttgctgcacc gccatctact ttcaatacag gaatcttgat tccagtgtca tcttgcatcg
     6061 tatcgatgat atctctcact tgataagcaa tcgactgtaa agtagcttta atgatatctt
     6121 cttttgtcgt accacgtgtc aagccaaaca tagcccctcg tgctgcctga tcccaataag
     6181 gagcacctag cccaacgaat gcaggaacaa cataaacctc atcttcgctt gtagagcgtt
     6241 tcgcggcgtc ttctgaatcg ctagcttttt gcaacatttg aagtccatct ctcagccatt
     6301 gaatcgaaga accagccaca aaaatacttc cttctaaagc atagtaaacc tttccattta
     6361 tgccgtaacc aattgtcgtc aataaattat ttttggataa ttgcggtttt tcaccggtat
     6421 tcatgacaat aaatgaacct gtaccataag tattcttgac catgcctggt tcgaatgcca
     6481 tttgtccgaa taatgcggct tgctggtctc ctgccatccc tgaaattggt gcttcgcttc
     6541 cataaaaatg ataaccttgc gtataaccat aaacttctga gttagaagtg acttttggta
     6601 acatcactcg aggaatattt agtaaatcta aaatctcctg atcccaatct agctgatgga
     6661 tattgaacaa catcgtccga ctggcattcg agtaatcagt aacatgtgct tgtccatcgg
     6721 tcagtttcca tactaaccaa gaatcgatcg ttccaaacat caattcgcct ttttcagctc
     6781 gttcttgcgc gccttctaca tgatcaagaa tccagcgtat cttcgttgct gagaaatagg
     6841 catcgatcac taaccctgtt ttttcgtgga aaaattcttg atagccttca ttttttaagt
     6901 tatctgcaat tccagctgac tgtctggatt gccaaacaac tgcatgatag attggacgtc
     6961 ctgtatcttt ttcccatatt actgttgttt cacgctggtt cgttatgcca atcccagcaa
     7021 tctgatttgg tttgatccct gattcgatga acgctccggc aataactgac tgtacagaat
     7081 tccagatttc atttgcatca tgttccaccc aactttcttg cgggaaatat tgcgtgaact
     7141 ctttttgaga actgccgatg tgtcttcctt tcttgtcaaa gataattgct cgtgagctag
     7201 ttgtaccttg gtcaatcgcc atgatatacg ttgattctgt cattgtgatt ccctccatta
     7261 tatgcaaacg ctttctttaa ctactcttat catactactg ttttaaaaaa agaaactgtc
     7321 aaaattttat tctttttttc tttttttgac agttttttat tatgaagcta ttttttaatc
     7381 aattcttttc ttttttcagc gtctctattc ttcttttcaa tcgaataatg tcgtaaggtg
     7441 acgcaaattc tgacagataa tattcacact gttttttgtt tctctggcaa aatgtgatca
     7501 ccaaatcata ttctttacct tcttcataac ttccaatctc aataccgccg attcccctca
     7561 gttctctgat cagatattgt tgaaaagcaa tacgataaat cggcagactt tctaaatcga
     7621 ttccaatcac tactggtttt gcaatataaa aatcgatcat caaaagaaca ttggcatact
     7681 ggatcatcag gtaactaagc tcactatctt cagctctttt cctcggcaat tgctgcaccg
     7741 ctgtttcctg taatttcgcc agtaactgtg ttaaggaatg cccgagcatt ttctgctgct
     7801 gttccaaaag ccgatcacgc tcatagactt cgatctttcc aacaaagaaa aaccattcat
     7861 tgttgatctg ggaaatttgg tagcctactg ccttttcttc tttgattttc aaacgacgag
     7921 gacgataatg ccataacatc gtttcccgaa tgtaagtatc taaaacagcg gttggtaact
     7981 ttttgctccg tgtcaaatca tactgataaa atatttcttc gtctactatc gaaaaagaaa
     8041 caaaaaagct gtaaaacatc attgtttcgt atacattcag ttctgctgct gttcgactaa
     8101 gatgcatcac gatgattgga tcgattgctt gataaagccg gtccgactga tagagtaatt
     8161 tcttttcttt caaagttgcg tacgtacttt tttcattcaa tagtcttttt ttcgtaatcc
     8221 cgagccagca agcaatctgt gcttctgctg atgccgtaaa cgttgtttct aatacacggt
     8281 ccaatcctag aatgaacggt cttttttctg gtgtattttg caaaaaaagc ggtcgctgat
     8341 cttccgggat atattggaat agttcataat aaaaatatcg tatctgtagc tcttctccgt
     8401 ataattgacc gtttttgatt tgtaatccaa attcctctaa gagctggttc aattctttga
     8461 tctttcgaaa aacagaagac tcactgatat taaacgctgt cgctaatcga acgatggata
     8521 cttgctgatg ttctaataaa tagacgagca tcttaaactt caagctttcc tgcaataaaa
     8581 acaatagaat tttttccaaa cttacatctt cgtccaacac aagcagcgcc tgatactcat
     8641 ttcgctgtac ttctactttt ttttccatca ttcggccaat caattcaata tcttccaaat
     8701 attgatcaaa cgaggctttt gacagttgga catgctctct caaaccattg atagaggctg
     8761 ttcccccttg cagaacaagg tttcgcaaaa tcaggatctg taactgctct tttctttcta
     8821 ataatgcttc aatcttcata ggcagtattc gccacctttg accacaccat atctggcaca
     8881 cggtattctg gctggaaaaa tagctcatgt tcttctaact gttcaaacgt ccatccttct
     8941 tctactgcaa ttgtcagttg gtcaagaagg tggaacatct cttttttgct ttggagctgt
     9001 gctccaagaa tctttttttc agaatcaaat agaaatttga gtttaacttt gctttgattt
     9061 attgctgtaa gcttgtagtc tacgtatcct gtttcaattt tttggggata aaaaatgctc
     9121 tcttcttctg tcaaacctac gcttcctaag aaatagccaa accactgatt gccgactgga
     9181 cgataggttc cctgatcctt tgtctgtgag ccactaattg tcttgctgac atttcgtgct
     9241 gtacggacag cgttatttac tagagaaaca tacatttttt catttgttgg acgaaaagtt
     9301 acttggatcg catcgccaat cgcataaacg tctttttgag acgtttgcaa gtagtcatct
     9361 acccagatcg ttccatcatc attcaatgtt aattgttctt cccacatctg attgtcgggg
     9421 cgagaatgat tagccagcaa aacaagatca tagttctcct ttcttttttc cgtctctaca
     9481 atagcggttt cattttcggt caaagacaca acttgttctt ctaaaaaaag tgaaagccct
     9541 tgatttttca tttcctttac taacggttct gtcatttcag gatctagata acgaaaaaga
     9601 attgactttc ggctttcgta aagatggatt tgtttttttc ttgatgccaa cccttcagct
     9661 atttcgattc ctgcttgtcc tgcaccaata attgcaattt tctttgcatt catgatcttt
     9721 tgctgaagag ccgttacgtt ttgtatcttt ttaactgggt gaataagcgt tgattctgca
     9781 gaaatatttc gaaacttttg atttgaacca gtcgctagaa tcaaacgatc aaaatccaat
     9841 ttttttcctc ctgctaaaac gacttttttt tctttcaatc ctattactgt cttttctgtg
     9901 taaacatgga ttcgatagcg gtcagtcagt tcttctttcg tgatccaaga aagttgtttt
     9961 tcactggtaa cctttccttt taatatcaaa ttgaacccgc tgggaatcca tcctaacttc
    10021 gattgctttt caatcaaata tatttcactt gttggcatca attggcgaag ataaatagcc
    10081 gcatgaatcc ctgcaaacga cccgccgaca attacaatgt tcatctgcat tcctctttct
    10141 tataaggtgt tctactgcta acttctcaat cagaaaacac agtattcata actcgctttc
    10201 aaccttatga tacatagcga tgcagcagaa tcaaccctct ccatcatacc gtgttctagg
    10261 tctgcttctc aataatgtac tagtggacct aattgaagag tatcatttct gaagagatgt
    10321 ttcaaaaaat gtactcttat aaaagaaaaa aactcgcctt ttacgtacct ctgtccaaat
    10381 aggaaacaga aaagaacgaa aagacgagtt ttagcgtgtg taatgtaata gttagaatag
    10441 acacatcatt tatttttaat tctttaatgc ttcaacaact gcttcgccta attgatgagc
    10501 agattgagga ttttgccctg taagaagccg cccatcaatt tgaaccactt ctgaaaaagc
    10561 tgctccagac tgatagtttg ctcctttttc tttcaaagca tcttctgcta agaatggtac
    10621 ttcattcgtt gtaccatttg cttcctcttc ttcattactg aacccagtca ctgtacgtcc
    10681 agctaaaata gattttccag actcatctgt aactggcaac aatcctacaa caccgtgaca
    10741 aacagctgag accagccctt catttcgata gatttgtgaa gcgactttcc caagatctat
    10801 tgcttctgga aaatcccaca ttgttccgtg gccaccagca taataaaccg ctgcataatc
    10861 atttgcatcc acatcatcag gacgtaaggt atttcccaag gcccgatttc tgaaatcttc
    10921 atcttcataa taggcccaat cgatttcatc catattcaaa acactttgcg gttctaaagg
    10981 tacatatccg cctttaggac tgacaaaatc tgcatcgatc ccatttttta ctaatacatc
    11041 ataaaaatga gtcagttcac ttaaccacaa acctgtagga cgctcgatct tttcgtactt
    11101 ttctacattt gttaatacga tcaatacttt cattttccca tcctctttct ataaaaattg
    11161 cgttattgat aacgtgcttt caagaaatta tataatgggc ttggcaaata aagctcttta
    11221 tatgcttctt caaaaggttt cgttacaaat tcatcctcac ggttttccaa taatttctct
    11281 gcccagtcag gatcgacgat catttgctgt ccaattgctg caagttctgc atatttcaat
    11341 acctgctcta catcttcgcg attgcggatc ccaccgacac caactaaagg gatctttcct
    11401 tgaatcgtgt tatggaccac ttcaagaatc gatttatcgt tgtatttatc agaacgagct
    11461 attcgatcat acatatttaa tgaaacatgc agataatcta gttttgactc acgtaatcgt
    11521 tcaagaagcc agatcgtgtc ttcaaaacgg atacctggtg tttcatattc ttctggtgag
    11581 aaacgatacc ctacgatgaa tggtttttta gcatattgtt caaccgctga gaaaaccgtt
    11641 gttaataatt cttcaataaa acgataacga ttttccaagc taccgcccca ttgatcctct
    11701 cgtctattcg aatgcggaga gaaaaattgt tggatcaaat acgtatttgc tccatgcaac
    11761 tcgattccat caaatcctga ttcaatcgct ctacgtgtgg ctttgccaaa tgcttggatt
    11821 gtttcaacga tttcttcttc actcattgct cgtggtgttt ccgcatcagg acgttttgct
    11881 gcaactgcac ttgcagatac aggctgttct ccagataaag ttgctcgact tgtcattcgt
    11941 ccaccgtgga aaatctgaat aatcgctttt gcgccttctc cttggattgc tttcgctagc
    12001 tcacttaatc gaggaatcat ttcatcatga gcgatgctga gttcacctgg ccagcctttt
    12061 ccgatatctt ggacatttgc agcaccagta ataacagcac caagaccttt agaacgttgt
    12121 ttataataag tgatctcatc ttcggtgatc actccattga aaaagctgag ttgtgtcgtc
    12181 ataggcgaca gcatcaatcg gttcttcaat tgaactcctg aaggtaaagt aattgattga
    12241 tcaaattgtg acattgtcat tcctcccata attcgctctg attaacctat cgagaatcat
    12301 ctatcattaa accactcgat tagttgtaca tgcaactaat ttgcttaaaa aattttattt
    12361 tttttgtaac ttatctgttg catcattcat tgtctgggct aattctttac ttgcttcatt
    12421 tcccaaaact gctttgtatg actgatgaag ttcttcccag ctaccatcga cttccttagc
    12481 aaattggcgt ccttgttctg taagataaac taatgacaag cgaccttcgc taactgtttt
    12541 gacgtatcct ttatattcta atttttcaac aaaacgagca atcgttgatg gggcggaata
    12601 gagcatttgt gcaatatctt tttgtggtat tccatctttt tcttttaata tcaataaaat
    12661 aaaaccataa gttggtgcca gacctgtcct tctaaacgct tcatcagcta atttattcaa
    12721 catgcgatct agctttttca cgctaaagaa aagacattca tctaacgctt tttcaattgg
    12781 cttttccatt tcatcccctc cattggcgta catatcatag tatgattttt attttttgtc
    12841 aaaaaacacg gaataatgtc cgcctcctct gtctagattt gaaaaaaaga agtagccatc
    12901 ttcaaagaat ttcggcttgt tttcgacaaa actccttctg gaacaccgtg aatcagaggt
    12961 tgtgaaaaag tcgtttagtt ttgagtatga agaaaaattt tgtgaaaatg gctttccaca
    13021 tttttgcata aatttggctt aataccgaaa aactggcttt tgaacaccgt ttatatcaga
    13081 ggttgtgaaa aagccgtcct gcatcaagta ataagaacag ccaaacaaaa atagttcctc
    13141 atattttgtc gttttttgag ctaatgccga tggtcagctg attgtacacc gtttattgag
    13201 aggtcgtgag cctgccgttg aactccgagt cacaaggagc aatttcaaaa aacagcttct
    13261 catgttttat gactatggtg acttgtgatc gaggagttgg caggagaata ctgtttattc
    13321 gtaaagaagg ggctgtgaca agacttttgt cacagtccct tatttccttt tattatagga
    13381 tttcgccatt tgattggatg acttgtctgt accaatcaaa tgattttttc tttaaccgtt
    13441 tctttgtgcc attcccttcg tcatcttgat caacatagat atatccataa cgttttgaca
    13501 tttgtgaagt agaagcgctg acgatatcaa tgcagcccca cgacgtataa ccaaataaat
    13561 ctactccatc tttgatagct tccttcatct gctcaatatg ctgtttcaaa tagtcaatgc
    13621 ggtaatcgtc ttgaacggtt ccatcttctt ctactttgtc tatagctccg agtccgtttt
    13681 cagctatgaa cagtggtttt tgatacctat catataattg attcaaagca attctcaaac
    13741 ccttaggatc aatctgccag ccccattcac ttgatggaag atacggattt ttaattcctc
    13801 cagttattaa gtttccttgg accccttccc aattttctgg atgagcactg actgcactag
    13861 acatgtagta gctaaaagaa acaaaatcaa cagggtaggc tgctaagatt tcttcatcac
    13921 ctacttctat ttttaagttg acaccttttt cttcaaacat tcgttgcgta tacgctggat
    13981 aatatcctct tgcttgaaca tcactaaaga acaaagaagt tctttgctgc tcaattgttt
    14041 tttgaatatc atccggatca caagaatacg gaacaagaag ctgatagctg atcatacaac
    14101 cgacttgtga acctttgata atttcatgac aagcttttgt agctaaagca cttgctataa
    14161 actgatggtg tgcaccttgg tatttcgctt gttccaaatg gggatggttt tcttcaatta
    14221 ctcctacgct aacaaaaggg tgatgtttta cacagttgat ctcattgaaa gtcatccaat
    14281 attttacttt atttttatag cgtttgaata ctgtttcagc gaaacgaacg taaaactgaa
    14341 taagctttcg attaggccat ccaccatatt ctgtaaccaa atataacggc atttcataat
    14401 gggaaagaga aacgactggc tcaatcccat attttagaca ttcgtcaaac acttgatcat
    14461 aaaaaaataa tcccgcttca ttgggtgttt cttcatcacc atgaggaaaa attcttgtcc
    14521 atgcgataga catccgatag catttaaaac ccatttcagc aaataaacgg atgtcttctt
    14581 taaagtgatg atagaaatca tttccgtgtc ttttaggata ataatacttg ctgttgggat
    14641 cctgcgccgc ttttacttgc tgatgcgtca tcatatgcca ttgatctgtc catttttctt
    14701 taggtaaatc gctgtcgaat gtatacacat cagacacaga taatcctttt tcatctacat
    14761 cccatgcacc ttcacattga ttggccgctg tcgctccgcc ccataaaaat ccttctggaa
    14821 attttttcat atcgatcccc ctaaacttgc tagttttctt gtttgattat ttttcttatg
    14881 actagtaatg atatttttga atctactact atttgtaaca ataaccattg tggtcggatc
    14941 atatccctct ttgattaatt cttgcaaatc tacttccatc aataaatcgc ctttacgtac
    15001 tttttttcca tcagatactg cactttgaaa gtatttgcca tttaaattca ctgtatcaat
    15061 accgatatgg atcagtatct ctgctccttc ttcactgact accccaattg catgtttcgt
    15121 tggaaaaact gttgaaactt ccccgtttac aggactgtag atttttccgt ccgttggaat
    15181 gatcgcaaac ccttctccta aagatccact agcaaatgtt ggatcgttta cttcagataa
    15241 atcaattatt tcacctgctg caggagtact taacactata tcctttgagg attcttcttc
    15301 gatttcatcc ttgtagccga aaagaaaagt tagcatacag gaactgatca tcgctaagaa
    15361 ataactagca actagccaaa caaatccttc accaatataa atcgggaaag acaaaatact
    15421 tcttgtagca actgccaaag caccggctcc tgcggcacca gctactccac caaatatagc
    15481 tccacatata caagcaatgt aaaatggtcg tttatattta agagtcacac cataaacagc
    15541 aggttcagtg attccaaata atgctgtgat aaatgcagat aaagataaac tttttacttt
    15601 cttatttttc gttttcaaaa ataccccaag tactgctcct gcttgcgcag ctacggcagt
    15661 catacatgct ggacctagcg tatcacgacc gtagaaagaa agattgttgg tcattactgg
    15721 tacaagtccc caatgtaacc caaaaatcac taacacttgc caaaatccgc cgataacagc
    15781 accagataaa acagggctaa agttataaac agctaaatat ccagctgcaa tcagctgact
    15841 tgcataatct gcaattggac ctacaactaa taaagtaatt gggaccatca cgatgataca
    15901 aatgaatggt gtcaataaat ttcgaatact ttcgtgcaag cttttgcgaa tcttaggctc
    15961 aatataagaa agaacccata cagctaaaat tgctggaata accgtacttg tataccttgc
    16021 tagaatcacg ggcatcccta gaaatcgcaa ggttatgcta tcactatatg cactgacaat
    16081 cgtaggataa acgagagctg ctgcaataac catagcgatg aaacgatctg tattgaactt
    16141 ttttgcagca gtatatgcca aaaatatagg caagaaatag aaaaatgcat cagctgctgc
    16201 attcaaaatg atataagttc ctgattcagt agttaataaa ttcaaactcg tacataaggc
    16261 gagtactcct ttgatcaaac cacttcctgc caagacgcct aaagtcgggg caaaaacagc
    16321 ggcgatcatg tctataaaag agtttaaaat gccttttttc tcagttcctc tctttccact
    16381 agatgcatca tcttctagtt tcatttgttt ggataatacg tcatacactt catttactgc
    16441 atttcctact acgacttgaa acattcctcc agcttcgact acagtgatca caccttctag
    16501 ttgctctacc ttctccttat ctgccttctt attatctttt agggtaaagc gtaaccttgt
    16561 tgcacaatgg taaacattgc tgacattttc ttcgcctccg acaaatcgga caatatcttt
    16621 cgctaattgt gaatagtcca tttttacttc ccccttgtta attaatgccg tagcaatcct
    16681 tcttacaaga taagtatagt tcttagaaaa cggtttctcc attcaattag taccccttat
    16741 aatagcgaac gaaactgtac aatagtgtgt atggtttact tagccgagca aatcactcac
    16801 tgcttgaaag aaaacaagta tatcgtttct cttatagtca aaggagacaa aaaaaacacg
    16861 cttgatcttc ctttgctgat gtataagtgg tatctcaagc tgatactctg tgatttttgt
    16921 ttctacttgg cttttgataa gacagcacaa caaagtctcg gcatttaacg gaagcataac
    16981 taggtcagaa ctaggagaga ttagttgctg aatggaatct atctttttat ggatttttat
    17041 ttgattttct tctttgtagt aatgatcaag cagttttgtt ttcttttttt cttcaactat
    17101 ggtcagcttt tcacgcaaaa tctgataaat ctcccaagat tcaaactcat ctagaacact
    17161 aggtagataa atcaaatcat cccactcaaa aataagctct tgctcagtag tcaaaattgc
    17221 atcatattcc gttattttgt ttgattgatt tttgaaaaga tacactggtt ctatccggca
    17281 ttctttgatc cacatgttca tactttgttg aatctgtttt tttagtgtat tcaaaatact
    17341 aattggctgg ttactaacaa tcagtatctc cccttgtttt cgaaaaggat tcaacgcttc
    17401 ggcaagatac aaaacaaaat aagcgctttc agctaaattg ggacgtaatt gaaaatgtat
    17461 tgggaagaaa cgccgaatta aataaatttc taaaggataa cgggagatga tcttttgtac
    17521 aaaatgattc atggtattat gtccggcatc catcctcgtt ttcatttgtt tgatatgggt
    17581 tagcaaaaat tctgtttcat ggaacaataa cgaaacagga agatctaaaa aacgaataat
    17641 cgtttgctca aatgctctta gattcttctc tatttctttg tttttttcat cattaatcat
    17701 caaataattt aactctaata gtcgattttc aattgcttgt ttttcttgat tggaaaaaga
    17761 atagggtagt tctgatgata acttttttgt gagggtctct accatcggaa taaataatgt
    17821 atacctgttt gttttttcta aaagttcttc tcttcgaaga atggtcaaaa cgagataccg
    17881 agaaaatcgt atgaagtcct ctcctgaaac aatataatta tatgatataa gaacctcttt
    17941 taatgtcttt tggacagtcg gcatgatatt ggaaaaaatt gtactgattt gttgaggcaa
    18001 ccttgcgtgt tggatcaccg tttctgttcc aacaagagat aagaaaattc tttttgaatt
    18061 ttcttcagca atgattttta gtccttttgt cgacgaaact tccagttcta atttaggatt
    18121 gcgctcgatc caacgcaaaa tcgtttttat ttgtaatgat acggctgttt ttgaaagaaa
    18181 gaaaatgtct gctatctttt gcatactgac ataccctttt tgaaacaata agtacgtcac
    18241 aatagttgaa gctcggaaat ccatgctgga atagatttta tgtttcgtca cctcttgtat
    18301 gatttgctgt ttagtttcag attcaattga ttctaaaaaa taacctgaac gtttatttga
    18361 ctggataatg attttcttct cgtagatagc aagcatattt ttgatttcag tttggatcgt
    18421 tttcttgttg gtacctaaag cttcagctaa ttcttcgctt gttatccaat gattcttatt
    18481 tcttaatact acaagaattt ggagttgtcg tttatttaag tccatttgct tacacctcct
    18541 ctttgttata aacatacagg aaaaacctct caaatgacaa tagtcacttg cagaggttta
    18601 aaaatcattt gaagaaagat ctatattcaa ggtttttacc attagactgg ataatttgtt
    18661 tataatattc aaaactatct tttttcaatc tcctaaagct tccgctccca tcattttgtc
    18721 tatccacata aataaagcca tatcgcttcc tcatttcccc tgttcctgcc gatacgatat
    18781 cgattggtcc ccaccataag taaccaatga tttggcaacc atcttttatt gcctcagcaa
    18841 tttcctgaag atgtattttt agatactcac aacgttttgg atcgtggatt cgattcaatt
    18901 catctggtgc ttcatctagt ccaatgccat tttcaactat gaataatggt aattcataac
    18961 ggtctgtcaa ttcattcatg acataacgaa accctagagg atcaattggc caactccaag
    19021 gttcgggaga tttttccttc aaaaagggat tatcgaaacc atttgctccg ccagtatcta
    19081 cgcgcatttc tgcttctttt ttatagacag cgctacgata ataactaaat gcaatataat
    19141 ctgccgtgtt agcttgcaag cactgttcgt ctccatctaa cattactggt gcacagtctt
    19201 cttccttcca tgtcctgtaa atataagctg gataatgccc tcggcacatg acatcggaga
    19261 aaaaccatga attgcgttta aattgcagtg aaccaaatac attctctgga tcacagtcat
    19321 atgcataagt cgctaatgca gaaaagctac acataatacc catcttagca tttggatcga
    19381 ttttctttaa taattgagta gcacgggcgt tcgctagaaa catatgatga caggcttgat
    19441 aaatcacttt ttttgttagt gtagcaatgg gttcaagttt atctctagca gggaatgtaa
    19501 ccaatccagc agctgcataa ggcatacgaa aagcattatt gatctcatta aaggtcatcc
    19561 aatatttcac tttcccttgg tagcgttcaa agacggtctg cgcataaaat tcaaaaaagt
    19621 cgattaattt tcgattttgc caccccccat actctcctac caatgctaag ggggtttcat
    19681 aatgactaag agtaattact ggctcgatat catatcgatt taatgtattg attagtctgt
    19741 cataaaatac gagaccagcc tcatttggct tctgttcgtc tcctcttgga aatattcgtg
    19801 cccatgagat gcttgttcga aaagctttta aacctaactc cttcagtaaa cgaatatcct
    19861 cttcaaatcg atgataaaaa tcaatcgctt catgagaaag ataatggagt gattcgtcta
    19921 aagcaatctc ccaaatcttt ttttcttcat tccactgaat actaggatgc tggttcatga
    19981 tcccgataag cgtatctgtc acattaggta atttgccgtc ttccaaccat gcaccttcac
    20041 attgattagc agctactgct ccgccccata aaaaattttc tggaaatatt tcctgatcca
    20101 taactatctc tcctcgctat caacaatttc ttacttgaag tatagctcga ttcatcaaaa
    20161 caagatattc taactatttc ggcagaagag aagaaaaaaa ttgaatgcta ctcttcattt
    20221 ttaatattta tatataacaa atacataaga cgatccccga aagaaaacac tcgttaacca
    20281 tctaagcaaa cgatttattt tacttccaac acaaatgctt cataaggtcg taaatcaact
    20341 ttgccgtctg gtaactgttt cagaggataa tttgacacaa taacccttaa agcttcacgt
    20401 ggaacagtga acgtctgctt ttcactggac atattacaaa taacgagcca ttgctggtct
    20461 tcatatatgc gagaataggc aaagacatgc tcaggcgtct tttccactaa ttcatagtct
    20521 ccccagacga tgatgtcatg ttttctacgc aaatcgatca atttcttgta catataaaaa
    20581 acagaatctt gatctgctaa cgcttcttct gcattgattt ctttataatt tggattgact
    20641 cgaagccatg gaattccagt agtaaaccca gcatgttcac gggcgttcca ttgcataggc
    20701 gtcctagcat tgtctcttcc tttcgcattg attgattcca agatttcctc ttttgtaaat
    20761 ccagaagaaa tcctttcgtt atacatattg attgtctcaa tatcttttgc ttccgcgata
    20821 tcatcgattg ggcaattcgt catgcctaac tcttctcctt gatagatata aggggtgcct
    20881 ttcatcatat ggagcaagat agcaaacatc ttggcacttt cttttcgata cacatcgtca
    20941 tttccccaac gagaaacaat tcgcggaaga tcgtgattat tccaaaataa actattccat
    21001 ccttcatcac ctaaagatgt ttgccatttg gataatactt ttttcagttc agagatactc
    21061 aagggtttca aatcccattt gtctttgcct tcttgctgat ctaatcctat gtgttcgaat
    21121 tggaagacca tggataattc atttctttta ggattggaat aaagtttagc gatttcggta
    21181 gttgctcccc atgtttcgcc aacagtcatc agatttttgt caccaaaagt tgcttgattc
    21241 atttcctgaa gatactcatg cagtttaggt ccattacttg tgatttcttt atccggttct
    21301 ttgccaatca tatcgatcac gtccatacga aagcccccaa tacctttatc gatccagaaa
    21361 ttcatcattt gataaatctc ttggcgaacc tgtttatttt tccaattcaa atcaggttga
    21421 cgcttgctat acaagtgcag aaaatattgc cccgtttttt catccaactg ccaagcagaa
    21481 ccactgaata ttgagcgcaa tctattaggc tcttttccat tgaccggatc acgccaaata
    21541 taatagtccc gataaggatt ttctttattt ttacgggcct caatgaacca cggatgttca
    21601 tcagaagtat gattcaccac cagatccatg atgatacgga tatttctttt ctttgcttct
    21661 tcaatcagtt cttccatttc ttccatcgta ccgaattctg ccatgattgc ttgataatta
    21721 ctgatatcat acccattgtc atcattcgga gactcgtata ctggagaaag ccaaattgca
    21781 tcaataccta aatacgccag ataatccaac ctggaaataa tcccccgcaa atctccaata
    21841 ccatcttgat ttgaatcttg aaaacttctt ggatagattt gataaacaac tgaatggtgc
    21901 caccattttt gttccatgat tttcctccta taaatggatg tttctatttt ttgcttaatc
    21961 atttatttta gtctctctca ctaaaaattg taacgctttc aaatagctat tgcaccttgt
    22021 tatcggtaac agaataaaac aaaaaaacag ctgaggaaat tccctcaact gttttttgaa
    22081 aggattgatt tatgcaggga agaatgctga tttcacaact tctttataaa cggtgttcac
    22141 tcagctgtcc cttggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttttcgtt
    22201 tttttctcta atactcaggc cagaacactg atttcacaac ctctttataa acggtgttca
    22261 ctcagctgtc cctcggtatt agctcaaaaa acggcaaaat ataagaaact attttttcgt
    22321 ttttttctct aatactcagg ccagaacgct gatttcacaa cctctttatt tcacattcaa
    22381 tatccacatg aatcccacaa atacaaagaa cagaccatac atgattggag atacttcttt
    22441 tcctctttta gctgctaaca tggtaattgg atagaagata aaacctaatg caatcccatc
    22501 tgaaatacta taagtcaaag gcatgccgat caagatcaag aatgaaggga tcgcaatctc
    22561 taagtttccc cattcgattt gtcgcaaaga ttgtgccatc aatactccta ccataatcaa
    22621 tgctggtgca gttacttgtg aagtaacaac tgatagtaat ggagaaaaga acaagcctaa
    22681 caagaagaaa atccctgtag taatagctgt gatacctgaa cgtcctccaa cggcgatacc
    22741 agctgatgat tctacaaatg caccaacagg agaagtacca aataaggaac ccgcaagcat
    22801 agctgttgaa tcagaagcta aagctttccc tacacgaggc atcttgttgt ctttcatgaa
    22861 acctgcttgg tttgctaatc caactaaagt tcctgctgta tcaaagaaag tcacaagcaa
    22921 gaatgtcagc actacgaccc acatttgaag agaattgatg tcccctacat gttttaatgc
    22981 aactaaaaat gttggtttca aacttggtgc tgcggcaaca atttcagaag gcattggaat
    23041 caaacctgtg accagaccta aaatcgttgt agctgctatc ccgatgaaga tgccacctgg
    23101 tacacgccga acaagcaaga tagcagttac gactaatcca aaaatcgtca accaagtaga
    23161 accaacgttg agtgatccta atcctactaa tgtagattca ttcgaaacga tgatcccacc
    23221 ttcgctcaga ccaaggaaag cgataaatag accgataccg ccagaaatcg catatttcaa
    23281 atctgctggg atcgcatcaa tgatcatttc ccgcagcttg aagattgtga tcaaaataaa
    23341 aatcaaagaa gcaacgaata cgccggctaa tgctgtttcc catggaatcc ccattcctaa
    23401 acaaacagaa taagcaaaaa aagcattgat cccaagagca ggtgccgttc caattggata
    23461 acgggctaat acccccatta aaacacagcc gatcgcactg gctaatgcag tcgcagtaaa
    23521 cactgctcct tcatccattc cagaagcacc taatacagtt ggatttacaa ataagatata
    23581 agccattgag ataaatgttg taaatcctgc cagtacctca cgttttaccg tggtgttcaa
    23641 tttttctatt tcgaagtagg aaagtatttt ttccttcatc ttccatcccc ctaaatccga
    23701 acattataaa taaagtccgt taaaatttcg atttattaat aaaacaacgt gttttatcat
    23761 atactaaaac aaaacataag taaacaaaaa attcggattt taagcaaaat ccgaacgttc
    23821 catctgatta aatcccttta tctcaaagtt catgaacatt cactttggat tacaagaatt
    23881 tctttattgt gttaataaag tttcttcatt ctagtctcaa aaatcaatag tgtgatttga
    23941 ttttaccatt tcgtttctgt tcttctttga tgaattcttt tgtatcgaca ggattcagat
    24001 tatttagtcc gccaaaacct gtatcgacca tttttcccca gccttttgtc ttcttttttc
    24061 ctgatttgag attctttgct cgaagcttct cactatgttt tgctcgccga tacttcaaat
    24121 aaccttctaa tcttcccatt agatccactg tttttcctcc tttttattca atcagacgac
    24181 tcagcttttc ctataacaaa ataattgcct tcagggtcag aaaaattgaa agtcaagatg
    24241 ccattgttat ctgtgatttc tcctggttct tccatgttct cgtagatagt ttcaaattca
    24301 tccgtaaaaa atactaaaga aggcggtggt cctaatacct ctggtgaaaa cttctgcaca
    24361 aatacttttg ggaatacgcc taattctaat tcttttgaga tgctgagtac aatactctta
    24421 aattcttctg gcaattcgat tgtttcagaa atttctgcac caattttttc tacaaagaat
    24481 cggcaaatca tctctacgtc attgacataa agcatcacac gtgttttttg tatcatcatt
    24541 tgacccttct ttctatttga ttttctttcc tgtctataaa ctgattccat tatatcacga
    24601 aagcgcaaac accttgtgtt tgatactatt caagaaaaat aaagcaatag tttctaggat
    24661 tgctttcctc cacaaacttt ttttacaaaa aaaaaaaacg gcaatgaatg ccgctcttct
    24721 tatttcgatt cttttataat tctgcttctg attcatcttt ggttagtgtg tgtctaacac
    24781 tagttacgcc aaatgtttga tcgatttttt ccttcaacgc ttcgattgcc tgtgcgccac
    24841 gtgccgcttg ttcagcattg atggattcaa tgaccagcac cgctgcttgt gtttcttccg
    24901 tcagcattgt tctttgggct tcacgccagt tgagacagcg acagatcgct ccttgttcat
    24961 catagtagat gatttcttcc ggaagtgccg gagcatcgga atctgcacct aaaggcagga
    25021 atgattcgcc accttgggct tttcctaaat gcagatttcc tgcgatcttt tcgatatctt
    25081 ctccgccgca aggtacgcca aaagacaagg aaatactatt atagatatct acaagcggat
    25141 tgattgggaa aaattcccgt tcttgatttg cacgtttcag taaagcttca atagaagaac
    25201 gtgcaccttt tttagtcttg aatgtggtaa atgcctgacg ccactgagca atgacttcgt
    25261 tttggctgaa tgtttcttct gtaaggtaat ttcttgcttc ttttgctgcc tcattcaata
    25321 attgatgata cgtttcttca ctttcttttg ttacgtggtt atcgatacca tttacggata
    25381 agatttcgat ggttgcttca ggaaatactt cccagaaatt ggtatccact attacttttt
    25441 tcatctgttc acacctcttg attagaataa ttgcttgatg aatagatagt ctgtttgggg
    25501 atcatctccc atataaaaaa catgttcgct gtatttgata aatccttttt tacgataaaa
    25561 agcgagcgca acatcgttgt gttcccaaac accgagccaa atggctgttt tttgcttttc
    25621 ttttgctttt tcaatagctg tatctagtaa aaatgtaccg ataccttgat gtttaaattt
    25681 ttttcgtaca tagattcgct cgatttctaa aaaatgatca gccatttcct cagattgact
    25741 cgttcctgta tttagtttca ggtaacccgc aggctcttga tcgatactca ctaggaaaaa
    25801 atcggtttct ttatctttta gctcttctgt aagtttttct agtgcataag cttgtgtcaa
    25861 gtaatcagct aaatgctccg gtgaattatc ttttccaaat gtatctgtaa atgtttcgat
    25921 actaatttgt tgtagtaagt ctacatctga aggcgtcatt tttcgaattt cgtatttatt
    25981 cacggcttct tcctttcaat agatcctttt attgcctttt ttgacaaaat tccaatcttc
    26041 gctaacattc ttgcatactt tatttaaaag atattccaat tgttttgctt cttcgtcaga
    26101 aagaccattt aatgcgactg cattggagta gttgttttct cgaatgatga aaggatagat
    26161 ttctgctcct tttttagtcg gatagagttt cttgattttt ttattttctt tatcttccaa
    26221 acgttcgatc atcccattgg attctaattt cttgattgct cgggcagtcg ttgtacgatc
    26281 tactttgatc atttctgcta gtttttcttg gatgattcca ggattttcgc aaatacgtac
    26341 taaataaaga tactgtcctc ttgttaaatc gacttctttg aattcgatat tgctaataga
    26401 atctagcgct cgggcaatca ttccgatgtc tcgaaggatt tcagccattt catcccacct
    26461 ttattttgtt tgtaagacta gtttacctct ttttgttgca aatgcaacta ttttcaataa
    26521 aaaaatcaag ttctttttta ataactagta aagtatattc ttttcttctt ttacgaaaaa
    26581 gctcaatcac aatagatctg gtttttgttt tctaaaataa gtaactgttt cttcgttgtt
    26641 cgcttttctg atagctttgg caactcttct atactaaaaa atcccatgtt ggaagtttca
    26701 ttattctcaa taaacttggc ctcttcaccg tggatcgcac aagcaaaaac aagtttataa
    26761 tactgaaaaa gctgtggaat atctttttgt ttattcgtat caaaaactgc tcgcaactga
    26821 gtagtttcta ctgttaatcc tgtttcttca tagacttctt tacgcacatt ttcttcagga
    26881 gacaatccta tttcagcaaa tccaccagga agtgaccatt ctttcgtccg tttatcttcg
    26941 attagtagga ccttgccatc ttttttgata taggcacgaa catctacttt tggtgttgga
    27001 tgtccttctc caatcgttgt gagcgctttt atttcttcga aagtttcttt tcctatttca
    27061 ctgatcagtt ccaatgaaat cgtccggatc tcctcaaaac gctctttatc aaatacatct
    27121 tttccataat atagaccagc ttgagaaagt gcgagtagtc ttttgtattg agccaacttc
    27181 ttgttcattc tcttcccttc ttttttagca aatgtatgac tatgtaatca agtatagaag
    27241 actggtttaa ttacggcaaa taaatataat gaaatctgca ataaataaaa gaatgttaaa
    27301 aaactcgaga acaaaagaca ttcgacttct ctggggctgt gaataaatgg cgttcaaaag
    27361 cttgcatctg cggtaataag ccccaccaaa cgaaaaatat gacgagctat tcttgtctgg
    27421 ctgttcttat taattgaggc aggacagctt tttcacaacc tccccactaa acggtgttct
    27481 cctaccaact cctcggtcac aagtcacaat attcatcaaa catgagaagc tgtttttttg
    27541 aaattgctcc ttgtgactcg gagctgaacg acaggctcac gacctctcgg taaacagcgt
    27601 tcaaaagcca atttttcgat attaagccat ttctttgtta cttattgtca ttgatttctt
    27661 tttctaattt atccaatagt ggagtgtaat ccacttgagt atagtctgtt acatcaaatt
    27721 catatagtgt tcctaactgg aatttgtcat attttcggtc ggcgatgatc tttttgaatg
    27781 cttctttcgt aatatgattg gtccctttcg ttcgatcttc caatacgtca ccatagtgat
    27841 aatgatccat gatatagctc aaatgacgat ctgattcacg atcacgtttg taccgacgtt
    27901 gacataacac ttcaaagtct gctgttaggc gaatcgtgat cacctgatat ccgtatctat
    27961 ctgctaattc ttggagtcgt gctttttgct tatcactaaa aggatactcg gacacaatca
    28021 ctttctttcc tgcttccata tacaaatcaa gtgctttgta atagaaagga taaactttcg
    28081 tcctttcttg ttccgcccgt tcttccaaat tgttaaaccc gatggattct gcatagtatt
    28141 ccttgatttc atcgagcgtg atggcataca tatctggaaa tcgttcccgt agtttattca
    28201 taagatatgt ttttcctgta ccaggactac cagccagaag aatgaaatac tttttcatct
    28261 catttatttc ctaagttaat tggactttcc aagttcataa acagtccaat cttatgcttg
    28321 acaatttctt ttactgcttc atttgctgga atcacattat ggcgaagaga cttgttgatt
    28381 tctgtttcac taaaggtttc tttagctact ttcgtccagt tgactaaaag ctccgttcca
    28441 acattgaatt tgcggatccc gttattgatc aattcaggat aatcttcttc tttcacacct
    28501 gttcctccat ggataactag ggggacatca actactgcat cgatttcttt cagcaaggga
    28561 atgtttactt ctgtttttga tttgaattgt ccatgattcg taccgatagc aatagctaat
    28621 gcatccacgc ctgtagcttc tacaaatttc actgcgtctt ccggtcttgt atataccttg
    28681 tcattttcag cgacatggat tccttcttct gtaccgccga ttgttccaag ttctccttct
    28741 acggaaacac cgtgcgcatg ggcatattct actactgctt ttgtcttcag gatattttct
    28801 ttgaaaggca gatgggaccc atcatacatg acagaagtat atccgctgtc gattgcttct
    28861 ttgatatcat caaagtctcg ggcgtggtct aagtgcaaga ctgcatcaac catttcttct
    28921 tctgcaatag ctgtcattac attgactaac acatgcatgc cgatatattt agcggtatcg
    28981 acacttgttt gaataatgat cggcgaaccc atttcttttg ctgcgcctag catagcagga
    29041 agcatttcta ggttatgcgt attaaaagct ccaactgtaa aattcaactc ttctgctgtt
    29101 tttgttactt ctttcaatgt tgtatacatc ttttattgcc accttatctt tttatttgtt
    29161 tcctttgatc gttacttgtt ttgcaatttg tcgcatctca tccattttgc ccatgtagta
    29221 atcgatccct tgatcatcac cagcacgagc agcatctgca cgcttctggt tatagaggct
    29281 catcagtttt ttatacccat ctccgatcgt ctgtttattt tccaaactct tttctaaata
    29341 ttggatggct tgatcgttca catacaattc agaaaattta agtccaactt gttcataaag
    29401 tttcgctaat ttatgcttat cttgggtagt ggaaatctct tgtttcaatt gtgtaatctc
    29461 tgattgaatc ttttcggtcg cttctggcga tagggctttt tcttcttttg ccagaacttt
    29521 tttcggttct ttttcttttt tcttaaaaaa tccaaacatc attttcccta ctttcaatta
    29581 aaatgctttc aaaactgggc ttaagatcca ggcatataag agtgctgcgg ctactgtatc
    29641 tacatcggtt gctgtagcat tgatgaatcc catcgaatta aagatcgtta ctagcaaagc
    29701 gggcaataat gtaataaaga aaccatgagc aataccgcca atgatcgctc ctcggcgtcc
    29761 gccaactgcg ttgccaaata tccctgctgt ccctccggca aagaaattgg ttagcatacc
    29821 aggtaaaatc atcgctaagc cgaacatggg caaagtaaac atggcaataa tcgtaccaat
    29881 cgttgtcgta atgaaaccta aaatgacagc atttgggcta taaggaaaga aaactggaca
    29941 atctaaggca gggatcgcat caggaactaa tttcatagct atccctcgaa atgccggcac
    30001 gatttcacct aacagtagac gtacaccagc tagcaataca tacacaccca ctacgaattg
    30061 gatggcttgt aaaaaggcaa acatgatata gttttgatct cctgataatt cagaagcgaa
    30121 tggttcgcct gcaaataaaa cagtgatgat atatagcggg accatcacga ccatgacaga
    30181 caaataagta tcttgcagaa attcgaaatg tgtcggcaac ttgatatctt cgatcgactt
    30241 tttattttcg cctttctcac caaaaagctt agcaacaccg gcttcaaata agtacccgat
    30301 cgtacaaaaa tgacctaaag caatatcatt tgatccagtg attttacgaa tgatcggttg
    30361 cgctactgcc ggcatagcaa tggcgaaaca agctccgata aaaccaccga ccaagatcaa
    30421 tacgatccct cgcagaccgg caaaatagcc aaagactgtc gtcatcgttg ccatccataa
    30481 aattgcttga ccagttaaaa aaatgtactt ccattttgtg aaacgagcca aaataatatt
    30541 gaatacaaaa atggctaaga acgtcagtgc aatatctcta cctaaaccca aatcattcat
    30601 tgcttgacca ttgatagatt caatagaagg gatgatccct tgcatatgaa atccttcagt
    30661 aaagattttc ccgaaataag ttaaacttcc aacgatgatg ctcgaaccgg cactcaacac
    30721 ttgaaatcct aggagtgtct ttaacgtgcc agagatgact tgtcctgctg atttcttctg
    30781 cagtagtaga cccaacatag caatcaatgc aatggtgatc gatgcctgag tcaaaatatt
    30841 ttcgatgata aaattgataa cgcccatgtt tttaccctct tttctttttg tatttagata
    30901 actcccttat cttgcagaac tggggtgatt ttttcttcaa tttcattttt tgaaacaata
    30961 tttttcaaat agatcatcgc cgttttagga tcaactgcga acttttcgaa ctgcgaacga
    31021 aaattttctg ctgtgatgat cacatctggt ttcatggaaa ctgcggaaga aatatcacaa
    31081 tggtccagtt tggcttctac tcctaatttg ttcaaaacat cctcagcaga catctgtgct
    31141 gcaaaactac ttcctaaacc tgcaccgcat acgaataaaa tgttcaattt gttcatcttt
    31201 gttctccccc gtatcattta ttatttctca actactgaaa aaagcagttg tttgaaatct
    31261 tcgatatttg tgcattcaat caatcgatcc aacttctctt cgttgttgat caattcaatt
    31321 agttctttca tgatgttcag atgcgagtag gagtctactg ctgccaaaca aaaaataatc
    31381 tttactggat ccatttcagg attaccgaaa tcgattggtt gctcaatcgt agctacgctg
    31441 actcctaatt tattgacccc atcttctggt cgggcatggg ctagtgccat gtgtttgcca
    31501 atcacgatat acggtccgta atcattcact gcacgaatca tcgcttcgac atagcgctct
    31561 tcgacaatgt tttctttaac caatggtcta gcagctagtt caatcgattc ctcccaagta
    31621 gtggcttttt ctttgatcat gatattgctg tccgtcaaaa tatcttttaa cataggctgg
    31681 atctcccttt tgttgatttc taaattgttt gtttcgaata atttttctaa cttgccatag
    31741 atgattcgat ctactcggcc accactttct ttcatgacat ctattatttg agtgaataac
    31801 tccgtcgatt catgactgtt aggctctaac cgctgtagtt cttcatttct tttcaaaaag
    31861 tcttctataa ccaagatatt cgtctcagtt aaaatcggat cgacaactaa aagcggctta
    31921 tttcgataat tcaattgaaa ggtgctgaaa accagattga catccaattt atctactaat
    31981 tcgatttctt ttgaacttaa aactgcagta atctcgatct gaggaaactt ttctttcaga
    32041 ttctctgcta acagatttcc agtagccatc ccatggttac agacaacgac tgatttataa
    32101 acataattca agtctcgctt gattacactg gcgatcgtcg aaaagtggat gacaagaaaa
    32161 gctatctcgt cctccagtat cttgccacca attacttctt cgatcgtagg aacaaaatga
    32221 gtgactgcgc ggtagattgg gaaataattt gttcggatgt tttctttcaa tggattcatg
    32281 acttggatat gattcttgat tcttacaatc agtgcagcca tgtgcttata gagattttcg
    32341 aataaagctt cttcctttag atggaaaggt acgtgtgtct cgttctctac aaattggatc
    32401 aattggatcg ttagtaattg agcttgtacc cactcgattg aattggagat atctctagca
    32461 ttgaacgtct caatagtgaa acgaagataa tttctttcta cttgcgggac attgtccaat
    32521 tgaaattcct cgatcatgtg gtcaataaat gcagaaaagc tactgtcttt tatttctgta
    32581 tttttccaat taatggcaga gataaattca tgtttcctga ttcgctggat ccagatcaat
    32641 gtaaaaagca ggatttgttt tttataaata tcatcttctt gtttcaacaa cttattggaa
    32701 tagatctcct gtattttttt gatttccacc aaaggaaaat aatggagaaa gattttttga
    32761 gccggtgtta attgtgcatc ttgattcata aagaagtcgg tcattcccag attctgattg
    32821 ataaaatcat agatcatcgt acggatagat cgttctgaac ctttataaac cagtccttgc
    32881 tttccataac tgacgatctc tatcccataa ctgaccagtg ttgctcgtaa ccgtctcata
    32941 tcttcatcta atgtactttt agagatttga aattcttctt cttttttgtt tagatggatc
    33001 gggattcggc tgtaagcaac ggctaaaact agatcaaaaa tacgttcgcg ccgattcaaa
    33061 aagccgtctt tcacatcttt tatttcagtt tctaatcgag cttgcttttc cttttccagc
    33121 ttcaacaaaa aaactttgtt gcgaattgta cggatctccg gaagcttttt actgctcaac
    33181 cagatattcg cttcaagaac ttcctgacgg atcgtccgct gactgacatt gaactcactt
    33241 gctagatgtt ttgcttttac tggatattgg gctgtcatga ttctgtgaag tatttcaatt
    33301 gttcttgttt tcatagaaat gatctccttc tgatttcaag tatatggttc cgcttacata
    33361 ataaaaagta gatatttctg ccacttgaag tggaaagaaa gtaagcaaag caaacaaaaa
    33421 ataagccata tgctttttca tctcctcaac gaaaaagaca tacgcctatt attacaaaaa
    33481 ttttgcccat ctatacaaaa aagaggttgt gaaaaagctg tcctgcatca agtaataaga
    33541 acaactaaac taaaatagtg cttcctattt ttcgtttggt tgttcttatt atcgcagatg
    33601 caagctttta aacaccattt agtggagagg ttgtgacaga gatgttcagc cttgagcgat
    33661 aagaagcgat tttgaaaaat cgcttcccat attttacaaa atcgtgcctt attgccgata
    33721 ggctatctct ggcacactgt ttattcagaa aaaggagctg cgacaaggct tttgtcacaa
    33781 ctccgttcat aaatatttcc tgtcaatcca gcataatcgg ctgaataaaa tcggcttgcc
    33841 atcttttttc tttctcagaa aaatcaaaat accatatctg agaaaaatac tgtcgttgtg
    33901 tcttacttcc tgagatcaat cgcccatctt ttcgatcgac tgaataatca cagcatctga
    33961 aatcaattcg gatggcaaaa ctattttcgt ggatctgtcg ataatttcct acttcctgaa
    34021 tagttatttt tttagtatga tttcttacac cgtctgcttt gtttttttgc agaactttct
    34081 gatgttcggc aaacagcttg tctgtataac agcttctggc tttttcagga gtttcttctt
    34141 cccaagcgga ttggatcttc atgaatatct tttggatttc cagaaactgt gctttctttt
    34201 ctttagccgt tcctgacatg cgggacaata tctccgcttt cagccttttc ttttgtcttt
    34261 tattcttgac gatagaaaaa gcagaaaccc ctcctactga caaaacagct aatgcaagaa
    34321 atcccgtttg ctcataacta gaaggacctg tacctggacc ataataccca taagaatctc
    34381 tgccgtaaga acgactgcca aaataactgc ccccatatga acgaccacca aatgaacctc
    34441 tgcccgaact tcgaccgcta gatgtacctc caccatgacc acccgcgaca gcgaatcctt
    34501 gggtcggcag agcaaacaaa cataggccca ctaacaaaat gattgctaaa taattgattt
    34561 ttttcattgt cttcctcgtt atcttttatt acaaaaaact atttttatga tagccttgct
    34621 aaaaggaaga gtcaataacc agatgcataa actgtttttt tagcaatttt gtcttattta
    34681 gtcattgagt aacatcggac aaatgtatgt gtcggcagtc cataattcct ctatcttcag
    34741 catgaacaag cttgcagaca agagatctgc gcttcctcca ggagagattt ggttttcctg
    34801 acaaaagtgc aacagtgctg taaatggacg atttggtaaa gcactttgac tagcagcatg
    34861 agcaagtttt tgcatttcca ctaacgtttt ctgatcactg cgatgcaata cgcatgtgtc
    34921 atctagcgaa ctcatcaaaa ctaacaacat atgcatttgt ttctgtacat atgaatcatt
    34981 atctagctcc ttagctgctt ggattgcaag cctgatatgc ggatacccta atatcgcttc
    35041 ttcatatgca ccatttacgc catatttttg cttcacccta tgaccatgag tggttttttt
    35101 atgacgatat tttgtatcag gaaaagcagc aagcttgctg acatcatcaa gtattttcga
    35161 taaagaaaaa ggctgttcca tactgatttt ttgtgcaatg acactgatca atagccccat
    35221 tgcccaaatc gctcctttat gggtattgat gccatttgta gcaatcagca ttgccttttc
    35281 cgcttttcgc ccaatctcag caattctttc tcttaactcc tgatcaatag catgatgcca
    35341 tgctatttga gccatttctt caaaaaaagg agttagcgtc ttagcagact taagaaataa
    35401 agcatagtcc atatcggaat gtgcacccgc atccagtgca tccactaatc caggttttgg
    35461 acttaatgtt acttctgcga tcaatgcttc ctctaccaac gcagcgagtt gctgagtcac
    35521 tgcttttttt cgttcgtcat ttatcatgac actcactcct tcttcctact attacttgct
    35581 gaaacctttt ttgatagcta cccaaatgat atgaagtaac cagcttaaag caaatgcaca
    35641 acttgccgtc attagataag ctacaagaat catgactcct gaaggtaact gcagccaact
    35701 atctttaaac aataataaca gtacttggaa aacaaataca ttggctaaat acgtacgata
    35761 agcataaaca gacaaccatt tgaacaatgg caattttggc gaatcgttta caatcatttt
    35821 ggaagcgcct atgaagacag caaagatgat gactaaactg tataaacttt ggatcgtatt
    35881 caagtaaggt gcattcgcaa agctcaagtc tcctggatgt gcgagcaatg aacggacaga
    35941 aaagaacgcg ataactaagg caacaggtaa gaaaagataa cgtactctgt ccaaatattg
    36001 atagactttt tcgtgataaa caaatgcggc taccccataa atcatgaaac tagcaacaaa
    36061 ccgatctaat aagtaccagc tgtcatgata aggcccagtc aatacttgtg tttgatagaa
    36121 cagataccat gcgacatata aaatcgttga accaatcaat actggccata catatttttt
    36181 attagcaaat actttgtaac cgagccaaac aaaaaacggc atgaacaatt ggatctgcag
    36241 catcataacg gtataccata aatgaggcgc tccatcacct gtgacatatt tcagtaaaaa
    36301 gcttgcagta tccgtatacg gagtttgttg ttggactgct ggaaatagcc aaagataagc
    36361 cgtcgtccat agaagatacg gaacgactag ttcaaaaaac ttttctttca aaaattccca
    36421 ataagacaaa tgctgacttg tttttaccat attataaaca attgcaaaaa taaacattgg
    36481 tgctgtgtat ttaacgatga cgtaaaaggt accaagaaaa gctgtatttg ttgcgctgtg
    36541 gctgtttgcc aaagcaaagc tcaatatcgt ttgcatcata acagccgtta cagctgatac
    36601 tttctggaag tcacttacgt tgattttgat ttttttgttt tcaaacatct ctttctctct
    36661 ctttttcttt attaccaaag ttctttggtt aatttaggtc cttcgactgt tttcaataga
    36721 atttttttct ctggctctct caaatactct tttagtgaaa aaccgccttg ccgagtcact
    36781 acttgggtat cgaattgctc aaaaaaagca ggatgcgatt gaattacctg aattggcaat
    36841 tcaatcggtg aatttgcgta cagcagcaaa tcaaaatcac tggtttcttt tactgccgga
    36901 atcccagtcg ataattcaaa tcctaagctg ccgccgattc cccatttaca tcctgataat
    36961 atgtttctag cagcatgaaa ttgctgataa acaggataat ccatgatttt ctcttgtcta
    37021 tacgattctt gttcgatgat gtcccacgga tgaatgactt gtttgacggc atgttttgag
    37081 atttctgttg accatctctg attgcgggca ggtccacgta aaccaatcgg tagtatttct
    37141 gcgtgatccg tcgacgcacg acgaactgtt ccgaacatct tttcagttag aacgacccat
    37201 tcaggcaagg tgaacccttc tatttcatgt ttatcaaaaa ggacaatatc atgtgcttga
    37261 atcatttttt atgcatccca ttcttcgtcc attttggcac ggactttatt tgtttcgaca
    37321 cgtccgaatg ttttggctgt ttcgtttgtg tatcgatttg tcagcatcgt gtcatttgta
    37381 ttgcggacat cttgaatggc ttcattgatt gcttcgatga ctttgctgac attttgttct
    37441 gaagcagctt ggtcggtgat atcttctagg aaatgataga tcgctcctag ttttgtataa
    37501 ttattgatat cgtaagcgat agctggaact ttttcagcag ctgcttcgat ttcagcaacc
    37561 gaacgttttg tcacacgtgc agcactttct ttagacatcg cttggacggt aatttcttta
    37621 ttgtttaaag caatcaagcg gcttgattgt aatccatgtg ctaaaaatgc acctgaaata
    37681 gcatttcctg caatcaatcc aataactgga tgtccagctt gacggagttt tgcataagca
    37741 gcagcactat tagccaaagc cacatggata ccgatcaatt cttcgttata gccgtatgct
    37801 tggcttggta catcgatcac aacgatgatt ggccgtttca cttttttgtc tttatcttct
    37861 tcgtaaacgt gatccaaaat ttgtcccatg cggaaacctt caactaaacc aacttcaccg
    37921 tttcgtacac ggtacatcgg gtttttagga tcaggtacga ttgatgtcac gatggcttct
    37981 tctatgaatt tacttgattt accataatac acagtactaa tagaagaagt tggatttttc
    38041 atgccagtta gtgccgcaaa ccaatcgtaa ccaacacttg ctgcagctgg ttcatttcct
    38101 tctgctactt ctggtaagtc aattgatttt gcttgatggt tagcaattgc ttcattgtaa
    38161 tcgtcaatgg ccattggttt tgaaagatct aaagaatcaa gtaatgataa ataaaatgct
    38221 gagctttcac tgcgatgcgt gtcttttcgt tcgtccattg ctgcgatgac agcagctttg
    38281 attgcttcta cactatcagt cactaattca tcaatgattt ttgattctac ccgttgacga
    38341 gatcctaatg tattccaaat caagtctttg tcactagaat caaattctcg aacgcctgct
    38401 tcttgttcaa taacttctgg tccattcaaa ccgactctcg ctttttttgt tgcgattaaa
    38461 taactcatta atgctgaagt aatcgacata ccgccaaatg atcctacacg tcctggaatc
    38521 aaaccgatga ctggtacata ttgttttaga gcaatgatta catttccgat ttctgaaata
    38581 gataacagtc cgtagtttgc ttcttgcaaa cgaacaccac ctgtatctag tacaatgact
    38641 ggaaagatct catttccttt tttattttct tctaacacat gttcaagtgc agcaacgatt
    38701 tttgcaccgg aaacttcacc gataccgcca ccttggaatt ttccttccat agcgattact
    38761 actgcttgtt tgccattgat cgtgccacgg ctgacaacga tcccgtcatc actttcaggg
    38821 acgattcctt gtgctggtaa acttggcgcg atgatttgat cgaatggatc aatcaattca
    38881 cgtccattgt caagtagagc gttcactcgt tctctggcgt ttaattctac aaaactattt
    38941 ttcatctgaa agttcctcca ttgcttgcgt caatcgtagg tacacaactc ctggtgtagc
    39001 accgaagtca ttgatcacga tatctgctga aattggataa cgatcaaaaa atctcgttaa
    39061 tacattgttc catacttcgc cgaagccatc gcttcctgta caaattttta cagtagattc
    39121 tgttttttct gttggaaaca tcaagatttc caaatctcct gaaccgacca ctcctacatg
    39181 gattttctta ttgatcggct gtttcgttgg atatttataa gctaattgtt ccatggatct
    39241 ctcccctttt tgtaaaacag agcatacacg ttctgcttat tcgtctttct tcttccatct
    39301 gggtaaatgg cgacaaaagg tttttctcgc caattttatg accaagttct gaatttcatt
    39361 ggtggttcat acaggccatc tgaccattcg atcaaatcat cgatggtttg tgcagctaat
    39421 aatgaacgtt tcgcttcact acgtttgatg cctagatctt ctggtaattt cacgatgccg
    39481 gctgcacgca agctatctag tgattctttc gtgctagtca ttccaagagg tgtaacaccg
    39541 ccaattgctg caattgctgc ttgtcgttct tcttttgttt ttgctttgta caaataagca
    39601 attccttctt ctgtcacgat atgtgtagta tcttcgctat aaatcatcac tggtgcattg
    39661 gctaagcctg cttgtttttg aacagctagt gcatctaatt cttcgacaaa gactggtttt
    39721 ttatttgaac catatgtttc aaccatttga acaactaatt tttggccttt ccctaatgga
    39781 tcatcattgt cgcgaagtga ttgccatgct ggtgtagaat gacgacgacc gccaggatta
    39841 tgtcccatgt taggcgcacc gccaaaacca gataaacgtc cttttgttac ggtagaagag
    39901 tttccttcgt aatctaattg caaggttgaa ccgataaaca tatcgatggc atattgtcct
    39961 gcagcttgtg ataatgttcg gttactgcgc atcgtgccgt ctttaccgac aaaaaagaca
    40021 tctgggcgag cagcaatata tttttccata ccaacttcgc caccaaaact atggatgctt
    40081 tcaacccaac ctgattcgat ggctggaatc atcgttggat gtgggttcaa tacccagttc
    40141 ttcgcgattt tcccttttaa ccctagtgat tcaccataag ttggcagtaa caattcgata
    40201 gctgctgtat tgaatccaat accgtgattc aaagattgta cgccgtgttc tgcgtagatt
    40261 cctttgattg ccatcattgc cattagaatt tgcaattctg tgatgttttg aggatctcgt
    40321 gtgaataaag cttctaattg ataaggttta tctgctttca cgacaacgtc tacccagtca
    40381 ccagggatat ctacacgtgg taaagaatcg acaacttcgt tgacttgaac gatcacgata
    40441 ccatctttga aagctgcagc ttcgatgatt gttggtgttt cttccgtatt gaaacctgtg
    40501 tacaagttgc cgtctttatc tgctttatct gcagctacta aaacaacatt tggaatcaaa
    40561 tcgatgaata agcgtccata aagttccaaa taagtatgga tatcacctaa tttcattgtg
    40621 ccgtcttcaa tcatttgggc gacacgcaag ctttgtgcac cagcataaga gaaatcgatc
    40681 ttgcttgcga ttcctttttc aaaaatattc aaatgttctg gtcgtgagat actagacatg
    40741 atcaaatgca gatcattcac tttttctgca tctacttttt caagactttc tgaaagaaaa
    40801 cttgcttgtt tttgattgtc gccttctaat acaactttgt cgccgggagc aatcagtttt
    40861 tccattactt ctacgatttt ttcagtttct acatacttgc cattgagcaa gttgctgatt
    40921 ttttccaatc gctgcgcttt tttttcgcgt ttagaagccc agttgcggtt tggtttttcc
    40981 ataattagtc atctccattt ttgatataga aacgtggcgt catcaatacc atgttggtct
    41041 agattgatcg tacggatccc tttatctaca ccaaattttg tatgaatcag ctttgtcaaa
    41101 gtattgccgg ggggaaattc aatcgtcaga tccattccta attcttttgc caagtccatc
    41161 atttggttcc actgcacagg gaaagctagg ttttccaaca aatctttacg aatctcttct
    41221 gcgtcctgag tggctcttcc atccgtattt tttatataca aacatctagc atcagataga
    41281 tggacagact gcatatacgg cttgaatttc ttgaccgtat cattcatcaa gagacagtga
    41341 gatggcacag ggactttcag cagcttcgct gtttgcgctt ggtgccgttt ggcttctgcc
    41401 aacgtaacgg ataaaccttc gatactccca gataacgtat gttgcatcgg acaattttca
    41461 tttgacagat aaactggatg gtcaggatca aatgtttttg ctacaatttc ctcagcttcg
    41521 cttctcgtca cacctactac tacgcccatt gcataaccgc ttggataagc ttctttcatt
    41581 aagactgcgc ggtgatagac tagttccgct gcattctcaa atctcaaaga tcgacaagca
    41641 acagctgcac aaaaagcacc aatcgaatgt cctgctgcaa tatcaggctg aataccgttt
    41701 ttttccattt catctaaata aaatactgct tttgtcagta atgctagctg gataaaaacc
    41761 gtatcttgat agttttcttc tttatcaact aaccgatacc ccgtcacatt ttctaaacgt
    41821 tccatgtaca tttcagggat atccgctaac atccctaact tctgactgcc ttgtccagga
    41881 aataagaaac ctgtactcat gagcctcgcc tcctcagcac ttactttttc cactgctatc
    41941 ttagtcaatt tctgctataa taaaaaatgc caaaaaaaga aaaaaccata acaaaaaggt
    42001 atggatggga tggataatta tgaaattgct tcatctgcag tattttaaaa aagtattcga
    42061 aacaaaaaat gttacccaag cagccaaaga attatttatt tctcaacctg ccttgagccg
    42121 tgcgatcaaa catttggaaa acgaattagg tgtccctttg ttttaccata gcggcagaaa
    42181 cattgagacg accgtttatg cagaagaatt ttatccatat gcagtcaaga cattagacac
    42241 attagaagaa ggaatcaaat tattgtcttc ggtcaatgag aagatcgtta cttccgtcat
    42301 attgtattta gaggtagcct ctgtttctat tccgaacttg gtccgaattt ttatggaaaa
    42361 gcatcctgat atccaattaa cgatcatgca acatcattta ccagatgata ttaccgatcc
    42421 tgtactgtac atcacttcgg agatgaagcc tggcatgacc aatattccta tcatgaaaga
    42481 acctgtttat gtcgccattc ctaagaaaca tcctcttgct aaaaaagaac atctgcaatt
    42541 agaagatatc caacgcaccc ccatcttgat gctgtcaaag agcaacgcct tccgtcatac
    42601 actggatgca gcacttgaag cgaaaagtat cgaattgacg atcagttcga cgacagatga
    42661 tcctgcgaca ttacgttcta ttgtcaagca aggattagct gttagctttt tcccaaaagt
    42721 ttcttggtct tacgataaaa atgatccttt cgtcttgcgg gaaatcaaag atttgccctt
    42781 gacacgaaca atttatttat cttctagctt cacagaagat catcctttga tcaaaatgat
    42841 tgcaaaaacg atgcgggaat tttatttagg aaaataaaaa aaaaagagct gggacaaaat
    42901 tcttgtcgca gctctttttt cgaacaaacg gtgttccaga agtaatttct tagaaaataa
    42961 gccgaaattt cccaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
    43021 ctaaacattt ctgtcacaac ctcttctcta ttgtcctaga cattcattgc gttgatttaa
    43081 aacttccatc aatcaaatct gctagttcat ctagcttttc tattttacgt agatcaatag
    43141 agatccaatg ttctttattc atgtgatagg ctggaaaata cccctctttt cctcgcaaaa
    43201 ttccgccaag ttcaggatcg accttcaaat caagaatatt gatgatccta tggtcacttg
    43261 catcatcata gattttttct tctggaacag acatcaggac cccaaaccat tttttgcttt
    43321 tttgatgtct gaatacgcaa taatcaggaa attttttaaa aatatattct ggagacaaat
    43381 tgtatgtttc attgatgtga tcgattagtt cttttttcgt tagcatctgc ttccatctct
    43441 ttcttattga ttcgctttat tcaaagatac cacgtaagaa tagaaaaatg aatacaagta
    43501 gttgctaatc aaatacttcg atatttttct tttttatcaa ctcttaaatg ttcaatccgc
    43561 ctgtgttggt cttgctacca tctactatta aaatgtttat tattgtttgc ccacctattt
    43621 tttgatttta gtggtatcat ttttcaagtt catgatgaaa ttcttatcat cagtaaatgc
    43681 tccagaaaaa taaacaatga ttgaaaaatc agttgaaact tgaccaccgt ttattttctc
    43741 cgtattctta gaatagaagg gagtctgatt gatgtattca ctaatgaaaa aaatcattac
    43801 cgaaaaagat attcgacgtc atgtttcttt agtagaacaa ttgctgaatc atacaaagat
    43861 aaccgtaaat gaattagctg aaatcatagg gacaaccgaa cgaacgattt tttctgacct
    43921 tcaatctatt cgctctcagc tgcccgaagg ctgggacatc atcagcgatc aagcaggaat
    43981 cagtttgcaa aaccagcaaa atttattaac aaatgacctt tgggagattt ttttcaaaca
    44041 atcagtcagt gtcgaactgc tgaaaaatct gctttttaca aaaaaagttg ctgtccctga
    44101 ttttctagcc gaccatgggc tttcttatgg gacattgaaa agacatgtga ctaaaatcaa
    44161 tcagcgttta gcaagttacg acttgcagat cgatttgacg aaatatacag cttgtattct
    44221 tggaaaagag cgtgcgattc gtacttttta tcatcgattg ctgattcctt tcactcataa
    44281 caactatttt ttcgaggatt attcgatcca tgaatcccat tattttcaat ttttaagaaa
    44341 cctcagtcaa actgaactag cggttgaaac agaagagatt ttcggaacat gttggttctt
    44401 tatcaatacg atccgtataa aagcgaattg ccgattagat tcttctatcc ataccaacag
    44461 cacactctcc tccctgtatg actctgcatt gaaaaaattg tatttaacgg aaggtattta
    44521 tcttaaagac actgagcttt cttttgcttc tttctgcttt ctggaaagtt ggaattacaa
    44581 caataactat ggacaagaaa ccgctcgctg cctgcatcat tctccatttt tagaggtcct
    44641 cgaaacgttt gtagaggaac tagcttcaga gctgtctctt gatcaactaa aaaaaacatc
    44701 tctgactgat aacttggctc tcctcatatt aaaatatcat gaatcgccga tattgattga
    44761 agcattggac cgtcaatacc atcgtttttt agaaacatat gatcagcaat atgcacactt
    44821 gtatcaacag aaagagaatt tactgaacga tctaaaaaaa gaaatagaaa tcaatgaacc
    44881 aaattacttt ctccaactct tgtctctact gatccaacaa acgatttttt ctatcaaacc
    44941 gaatttgttc aatgtctatt ttattttcca aggagaacct tcatggaagg cgttcttgca
    45001 gcaggaatta aaagattact taggaaaacg tgtctgcttc ttacctatcg aactaaccga
    45061 tctttccact atttcttttg aaaaaacaga tatcttgatt tccaatttcc ctctagattc
    45121 tctagatatc cctattgtat acatttcttc tattccaacg aaaaatgagc tcaatcgttt
    45181 gactgaactc actttcaaaa cttttttata gtaacggcta gcgactgtat tctctagctg
    45241 ttttttcttt tttgcgaaac agtttgttca tgatcccttt ttctatttct acaactaaga
    45301 atagaagaat cacatttgct ccaatcaata actgttgtgt catcgttaaa cttactgtac
    45361 cgatcagctg ctggccgact ggtagaaaaa taaccatccc ttgcaataaa gcaaggatac
    45421 ctaatgaaag aaaaagcgct ttgttttgca aaagaccttg gttcaatgac ggcttggaca
    45481 actctctgca attgatcatg taaacagctt gagctaagac gatattttgc agaagaatgg
    45541 tttgctgtaa gctttgccct tcaaactgca tagccaataa ataggcagga accatgatca
    45601 gtagggagac atagatgatc cgaaacaaat gataaccaga taggattcct tgctttggat
    45661 ctcttggtgg gcgcttcatg atttctggat ctggcgactc gaaccctagt gcataagata
    45721 aagtgattgt cgttaccata ttgacccata aaatctgtac tggggacaaa ggtagcgggc
    45781 gattcagcat aagtgcccaa atcaagatca atccttgagc taatgcagtt ggcaagaaga
    45841 aattgatggt tttttgcaaa ttatccaaaa tccgccggcc ctcttttacg gcttttgcaa
    45901 tcgtatggaa attatcatct cctaaaacca tatcagctgc ctgtttgctt acttcactac
    45961 ctttgattcc catagcaatt cctacatcgg atcttttcaa agcaggtgca tcatttacgc
    46021 cgtctccggt cattccgacg atttctccat ttttctgcaa agctttaaca attcgcagtt
    46081 tatgttctgg tgtcgttcga gcaaagacat cgactattgg cacttgctga atcaactctt
    46141 catcagacat aagatccaaa tcgatccctt ctaaaacttt ggcagtatgt tttaagccaa
    46201 tttgttccgc aatcgctttt gctgtgtctt tgtgatcccc tgtaatcatt ttcactgaga
    46261 tccctgcttg ttggcattcc tttacagctt tgactgcact ttcttttggc ggatcgatca
    46321 atccggcgat ccctgcaaaa gttaattggg ttaaagtctc atgcgtgagt tcctgtttcg
    46381 aatctacttt tttataagca aatcctaaaa cacgctgacc ttttctcgca aattctgcag
    46441 ctgtctgact ccatgcttct tgttctgcag aagaaagagt tgatttagca aataatactt
    46501 ctggtgcacc tttgacgtaa atcaccgctc ctttttcctc cgcatgcatc gttgccatat
    46561 atttgtattc agaactgaac ggaatctttg cttctatttt tttcaaaggt agattggctg
    46621 tttctgtgtg tcgaatcagc gcaacttctg tcggattgcc acttaaattt tctgttttct
    46681 gttcttcttt tgtttttaat tcctgacagt ttttcataat ggacaacacg caggcttctt
    46741 cttttgcagc tacatccaca actgtcattt cattttttgt caatgttcct gtcttatctg
    46801 aacagatcac tgtcatagaa cctaatgttt ctaccgaagg aagtcctttg atgatcgcct
    46861 tttcttttgc catttcatgt actccaacgg atagaatcat ggtcaaaaca gctggcaatc
    46921 cttcgggtac cattgacacc accaaagcaa tcgtagccga aagaaggata tgccaatcca
    46981 taccatatcg aaatgtcgta aaaataacta agaataggct caaacaaaga atgccttgaa
    47041 aaatttgttt gtttaatcgg ttgatttttt taatcagagg cgtcgtttgt tgttcgaccg
    47101 attgcaatgc ttgattgatt ttaccgattt ctgtcgtgtt tcctgtttct acaacgacac
    47161 cgactgcaga accagattgt acaagcgttc ctgaaaaagc catatttttc tggtcgccga
    47221 tcgattcttc ttctgtcaaa acggcatgac ttttttcaga tgcttctgat tctcccgtca
    47281 aaatcgattc ttccactaat aaatcatgtg cttcgattaa tcggatatct gctggaacga
    47341 catctccggc acgtaagaca atgacatctc cttttaccag ttctttcgca gggatttttt
    47401 cttgcatccc gtttgatagt actatcgctt cttgtcccat cagctgcttc aaaccattca
    47461 atgattcttc cgcttttctt tcttgccagt aactaacgag tccattgacg atcactacga
    47521 ataaaataat acttccttca atatattcac tagaagcaaa tttcaaaaaa gaggcaatga
    47581 tcaacacgat catcagcaga tcagtaaaat gctttgctat ttttttccat cgttttgtct
    47641 gctgccttgc ttttatttca ttatatcctt gatcagtcag gcgtttctgt cgttcttctt
    47701 gcgacaagcc ttctgttgat gtttgatagt ttttcaatac atcttctacc gtcaatctgt
    47761 accagttcat cctatcccct cctgtttaat tgatactgtt attatacgca gggaagtttt
    47821 ttcaggcgta tcgattattt caggggggtc tgaaataatt cgttttgcaa aatgtatctg
    47881 ggcctaactg atatttctat tgcttattct tttatataca aaatttaaga atacaaacca
    47941 gataaccaat aaaaaaggaa taaagcgtgt cgtgctttcg aataacaagc tgaaagcaat
    48001 tgaagtaaaa aacaccaaca aagcaagatc tttatatctt tgtgtagtgt tccgtttctt
    48061 ttctttaata aattgaacat gtgcacacaa gatcgaagac cacacaaata gaaaacaact
    48121 cgttgtcatg cctgccaaaa gctgaaaaaa caatcgacta tcagctacaa aaaaatgtag
    48181 acaaagaccg gcaaaaaata acattcctgt aattaaaata ccgtgtaaag gtacgccttt
    48241 cttagaaagc gcacaaaacc aatggggcaa acttttttct tttgctgttt gatataaaat
    48301 cctgctagta ctgtagactg cactatttgc tgaggacaaa gaagcaacca aaatcattag
    48361 attgacaatt gctgctccgc cttggcaatg gatcacttgc agcatagtca ctaaaggact
    48421 ttcttttaaa tctaaacaat gccatggaac aacaaataaa atcgacaaaa tcgttcctat
    48481 atagaaaaag agaatacgcc aaggcagaca gttgattgct tttttcaaat tcttttgcgg
    48541 atcacttgtc tcgccaacgg ttaagccgat catttcaatt ccagcaaaac tgaataaaac
    48601 cataggaaat gagactacca ttccagcaaa gccaaacggt agtaattcat cgatctgtcc
    48661 aaaattttct ttgattccga tagactgggt atgaaaatct gagacactca atcctatacc
    48721 caataagata aaaaggaaaa tccccaagat cttgatgatt gagaaaaaag attccaattc
    48781 accaaaccac ttgactgcaa caaggttgac tgccattaag atcattagtg cgctacctcc
    48841 gcttatcaaa gctggtacat gtacaaacca gaaatggata tacaatccta ttgctgttaa
    48901 ttccgcaagt ccagctccaa gccagcaaag ccaataagtc caattgacaa aaaatgccca
    48961 ctttttgcct aaataatagt caacgaaatc actgatagag ttaaatgtac tatttgctaa
    49021 aaataaatca cccatggccc gcatcaaacc ataaatagcc aaaccgcata tcaaataaac
    49081 gagaataaca gctggccctg ctgatgcaat tgcctttgct gaacctaaaa ataatcctgt
    49141 tccaattgcg ccacctaatg cgattaactg gatatttcga ttagttagag tccgtttcat
    49201 tgccggtttt tccatcaagt tcaccttttt ctttttcaag cagcatggcg acttcctgtt
    49261 cccaagaagg tttctttatt ttttcaatac agagtggtac tacaaacaaa atgacactga
    49321 tggctgtgac tgcaattaga ttgatccatg ttccagcttt catcgtcagt ccgacagaaa
    49381 atccgattgc cgcaaataaa acaataacga cagtccaaac tccaagattg ctctttttct
    49441 ctttttttcc aattctaaat ggtcgattca actttgcatg tgtataacgc aatctcaaaa
    49501 ctgcgatccc catgatgaca tagacgatac agtagataac agtagttgaa tttacgagca
    49561 tcaaaaatgc ttgattgacg ccaggaatca gcaaataaaa aagtgcaaag attgagataa
    49621 tgacagactg agtcaaaata acggatttgg atacgtcaaa ttgattcgtc ttccaaaaat
    49681 tgaattttgg tggatacgca ccttttctag cactttctgt cactgtcttt gctggtcctg
    49741 aggcccaagc agataattgg actgctacac caatgaagac aagtaaacta aacagattca
    49801 caataagatg tggcaaacca agaatttggc aataaatcga aatagattgc gcgatattat
    49861 tcaattccat ttgtcctttt ggtacgacat ttgctacaac taaagcattc aaaatattga
    49921 cgaaaaatat aaaaattaac gcggcaaata ctccgcgaat atacgtttta actggatttt
    49981 ccaatcgagt gatatacaca gaggacattt cgataccagt aaaaataaac atgatcggcg
    50041 caaaataaac aaatgaggat gcagtcgtag tatctggtat taatttgtcc catgaaaaac
    50101 tgcctaatgt tccggttggt acgatcccaa ctttgatcca cgcagcaaac cctaataaga
    50161 gcatcatcgt caaaggcacg tacaatccta accaaacacc gacttttccg ccgatttttg
    50221 ccatatcaaa tttcaaattc aaaagagtga tgatccaata aacgactaaa atgacaacta
    50281 atgtaaattt gctgttcaaa ccaagcggca cattatcgat aatattacca aacaacggag
    50341 cgagtgctga agcgaccatc accatcccag gaaacatttg gacccataac agccatgaga
    50401 caacgaaccc ccattttttt cctagagcat tcgataccca aagttctgga ccaccttttt
    50461 ggggaaacat tccagcaaat tcaccagaga tcaatgagat tggaaaagcg tacaataata
    50521 ctgcaaccag catgtaaaaa atcatcgtcc agctcgttgc tgctacatct ggaatatttc
    50581 gtacacttgc tacaagggcg caggtcattc cgataaaggt catggtcgtg atttgctttg
    50641 atgaaccagt atctttcatc tttattcctt ctttcaatag cgacatatgt ttttgttttc
    50701 ttttattttt gttcaagaac agaaatgaca aggtttcatc gattcagtac aaaacccagt
    50761 atcaccgaaa catccattga acgatcttag tgaactgggt ttgatacatg aaacgaacga
    50821 tccaacttat agaacgtttt ataaaaaaga atatcttggc gaggaatata tgaaaaacag
    50881 ggggaaatac aattgttttg atgaagcaaa taattgtctg ttctccatat gtctcttgtt
    50941 attctttcac acaaaaatca gttctattgg atgatttcgt ttagtttctt ttccattgct
    51001 ttggcgattt tttttgtata aaaattgaaa attcgatcat cattcaaata aggagtcatg
    51061 atagccgcac gtagcagagt gattttctgt tcttttttcc attcttcttt cgttagaccc
    51121 attctttcaa caaaacgaat cggagaatca ccgtaatctt cttgtgtaaa tgtagtatga
    51181 gaagtaataa accgttcacc ataaacatcg ccatccatat aagaagaccg atcgaacatt
    51241 ttttcattta attcattgat agcgttcaaa tctgtacagc cttgttcttt gaatacccaa
    51301 ttgaccatat taaaatcagg atgattcaat ggatagactt cgattgtttt tcctttaaca
    51361 gtaaaagtca gatgatcgag aaattctctg aatctttgag ctgcctcgat tgaagcccca
    51421 atcaattgac catatccggt cacattcaat ggcaatacac gatgtgctgt ccagactgcg
    51481 gcggcggttg ctccagcttt tgatccttcc aaaatataag ctcctaacat atctggagcc
    51541 tttactgatt tttcaaacac atatggcgcg aaataagaaa tgatatttct catattttta
    51601 tgtttgatta cgattcctcc agcagcataa gggacatagc ccatcttatg aggatctatc
    51661 gtgacagaat ccgcttctga aatagctctg aacccttcat atacctcttt gtcaatcgtt
    51721 acgcagtggt ggaaaacatg atgttcttca aagaattcag ctaaggaagc atacggcaca
    51781 aattctcctg cttcgtttag aaacagtgaa cgcgcatagc cgccatatgc ggcatcaaca
    51841 tgtaaataga aatagatccc ttcatctttt aacctttctc ttaattggac gattttatct
    51901 acactgtcta cttgaccttc ttctgtcgtt cctacgacag caaccacacc tagaattggg
    51961 attttttgtc cagctaattc acgaattgtt ttttccaaag cattaatatc cattcgataa
    52021 tcctcttgaa ctgggatagc aaccatctga tctaagccga ctccgcaaat atctaacgcc
    52081 ttcatccaag aatagtgttt tgtttgaggg accagccatt tccctaatct ttggatattt
    52141 tttccgctac gggaagaagc tgctttgacc tcatccattt cttcgtccgt aaagctttcg
    52201 gtcatttcca aaatttcttc aacagataaa ttcaaaagtt cccactcaga catttttttc
    52261 actttttcag gcagcacttc cttgacagct aaaggaatcg atttgatgca acgggcgtac
    52321 cataatcctt caagattagc aatagaacca tctgctgcaa tgtgtcccca tccgtctgtc
    52381 atattgaata atgaagcaaa atcctgtcct acttctgctt ccatttgcga agttgccata
    52441 gaagattcca atgcaacatt atttgggttc cacagcatcg catagttgta agctaataaa
    52501 gcaggcatca atgtttcagc attcatttgt ccccagtaac ggcctgctga atgccatggg
    52561 atcgatcctg cgcgtaatcg ctgactgact tcatccaaga cctctatcat atggtcgcgg
    52621 gtggctagat aatctggagt gagcttatct ccttcgctaa ttgcaggcat atctgaagga
    52681 atataatttt ctctccatcc aagatgttca tctaccatct tgttcaacaa cattttataa
    52741 actggtccat tttcagcttt gtcgcctata aagacggcct tgatatccat atctttcatt
    52801 tttcttccct ccatattttg ttggtcaatg tcttgcagat acatgtgttt atctgtaaca
    52861 cctcttcact atagcgccaa aaatgagaaa ttttttttta taacatcaaa tccacgtaga
    52921 tttttacaaa caaaaataaa taaagtcgga tttttctgat aattcaacgc aaaaaagcga
    52981 acaatttttc aataaatcag tcttctaaaa tagataacct aaaaaaagcg aacaaacaaa
    53041 taaaatgact gtccttcatt cactaaacat atttttattt ttttatattg ttaaaaattc
    53101 acaatttatt ttttaataga attatctgtt ttccaaattt tataacataa aaaacctttt
    53161 ttaacggcat ttatatgctt tttcagaaat taactgatga tttcaaaaag aaaacatgtc
    53221 ataatatttc ccatattgtg caaaaaaata caaggcggag gctctactat gattgaagaa
    53281 tatattgaga aaaatatttt aagacagctg tttttatgtg ggcaattcta tgtaaataaa
    53341 gaagtcaatc tagaaaagct ctccaatctc ctgcatgttt gcaaaaccac tttactcaat
    53401 gatatcaata acataaaaaa agaatttgaa gaacagatcg cttatacaca tcgggaaaag
    53461 gaccgttaca ctttatattt ttcagaacat atacctcgat gcaagatcat gcagcaattg
    53521 tctcaaaatt cgttgttttt aaaaacttgt ctgctttatt tggaagaaga tgaacctgat
    53581 tatcttcaat taactgaatg tgaattcatc tctgtcagca aagcttattc attaaaaaaa
    53641 caagtattag cttactttaa tgactgcggg atcgaaatcg accgctactc ccctcgattc
    53701 actgaaatgg aaagacgtct gctgcttctt aatgtgtcgt atcgtttagg cggttttaat
    53761 tcttgggagc tacctgaatc gttttttgaa cgtgccgatc gtttcattga atccgtaacg
    53821 gaaaatagtg gcagatttta tgataaagaa aataaagaaa tcttatcgat tggttttgct
    53881 atcagtttcc tacgccagca agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa
    53941 atcaaaaaac gtcctattta caattatgtc gaatcagcat gggaaaacac tgatttccaa
    54001 acctattata aaaaagaaga attcgccttt attttgacac tattcaatct ttgtaattat
    54061 ggtttccatt cttatcagtt gattgcggaa gactttcaac agctacacca agtgtttatt
    54121 gacaatactc ccgaaataaa agaactagtt gctacctttg aatctcactt caatcaagaa
    54181 ttattcggaa accaaccgtt cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat
    54241 aactatcaat tattcatgcc agaaaaattt tatcttttaa atgaggaaca gacaaattta
    54301 tataaagaag tccagacaat tttttccagc tggtccagcc agttacctta tgatctacgg
    54361 ttgaatccta attgcatgcg ggcttttgtg atcgaattga gcggaattct ccgtctgaca
    54421 aaagaacatc tgactatcta tatcgtcaca aattcggatg ttcattatct gatttatcgg
    54481 gaagccttgg aagctgtaac tactttcgat tttcaagttg caccaacaat ttattcaagt
    54541 atatctgata taaagaagta tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca
    54601 ttatatacac cagatgcggt ccaatatgaa aatattattc ctatttcgat caatacgatc
    54661 gatcgagcta tcatttcagc tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca
    54721 cagcctctta tttgttgaag atgcccattt ctgtaacaat ctcttgatac acgtcaggct
    54781 cacaacctca cttattttgg acatctaggg gtccgatctc tttatttaaa aaataagaaa
    54841 tgatggttcg gataactacc aacgccgcta gttggaaaat atcctgcaag gtaggtttga
    54901 taatcgattc gatgatccca gctgaaacaa gaatttccaa acttagaagt atatagcctc
    54961 ccaacatctt tttaatagca ttgttctcgc ttactcggtc gctttgcact tttgtatgga
    55021 caaataaata tttcaaaaaa tccttgattg atagacaaac accccaaacc aataaacaga
    55081 tagcaaagat atcaagtccc agtacaagtt tttcgaaata tggaagaaga aactgcatca
    55141 actctgtcgt catcctacct ctcctcttct ttgtttttct ttgtcttcta tttcctgatt
    55201 caagaaataa gaaatgactg ttcgaatgat aataataagt gcaagcttca aaatatctgt
    55261 ccaagtcggc ttgataactg actcgatgat atcagcaacg actagtactt ccaagcttaa
    55321 caaaatataa cttgctagca gttttcttgt atctgcatta cgagtattac gttctgcata
    55381 gtttttcttg acatgagacc aatcaaaaag tttgaataga acaagaacga tcccgacaat
    55441 taagataccg atagaacaaa tatttaaaat agcaatgatc cattctagaa attcaattaa
    55501 atttgagcga aacatatacg ctacctactt tcttttaatt tgttcctatt cttttattgt
    55561 aaaagaaagt taccttgttc gtctatttaa aagttgttct aatgataacg gttcgcttaa
    55621 atttttcttt ttcactctcc catttttctc cctccatcca taaatcgtgg aagtatccaa
    55681 aacaacgtag caagttaaaa taaggcttag tccgtcatca gcttttcaaa gaagcactgt
    55741 ctcggtgctt tttttgtacc caaatttcta aaaatagaac catacacctc ctcaaatctt
    55801 ttcttcttac ctaccctatt tttatttaca ggaaaatcag acacttcttc ttttttgtca
    55861 aaatttcctt tttcttctcg tcctatcatt tggcattcta ataaaaacga actatccttg
    55921 gatatagaaa gagaaaacaa ggaggcgttt ttcatgagta aaaaattaat caaagtcggc
    55981 atcggtttag gtttgcttgc ccttggcgct gcatatctag gaaaaaagac aggactattc
    56041 gaagatgaca gtcatttata tgatgaattc gaatcaatct agcatcctat ttagttgaat
    56101 cactacggac atacttggaa gacattggag gaatcacaat gagaaaaagt aaagcaataa
    56161 tgattggtgc cggtattgcc aatatggcag cggctgttta tttgattcaa gaaggcaaat
    56221 ggaaagggga tcagatcacc ttttattcat tggatgatca cggttcaaat gatggtgcac
    56281 cgaccgacac tgtagtggat gaatattgga ataaaaacca tccattagaa aaccaaaaag
    56341 gctatattgc tcgaggcggt cgcatgctta attaccgtac ctatgtagat ttgatggatc
    56401 tgttaagtcg aataccttct gttactgagc cagggatgac tgctgaagaa gatacgagag
    56461 attttgatgc aaaacaccga acctttgaca aagcacgttt gatggaaggt ggcaaaggaa
    56521 tcattgatgg cggaaaactg ggattcaata ataaagaccg tattcttctg actaaactga
    56581 ttgctatgcc tgattcagaa gaagaaaaac tagacaatat cactatcgaa gaatatttca
    56641 aagatgatcc acactttttt gaaacaaact tctggtttat gtgggaaaca acatttgctt
    56701 tcagaacacg tagttctgct caagaattgc gtcgctatat gcatcaaatg atttatgaat
    56761 tcactcaaat cgaacatcta gtgggcgtca atcgaacacg ttacaatcaa tacgaaagta
    56821 ttatgcttcc tttgatcgag tacttgaaag aacaaggatg caaaatcatt ttgaaccgcc
    56881 gtgtcactga ctgggaattc aaagaaacac cgatgcaaga tgaaatcact gtgactggtt
    56941 tgaaaattac caatacactg actcaagaag aagaatacgt tgtggtcgat gaagatacag
    57001 cagttatttt cactaatggg tccattacag attcagctac tttaggagat tacgaaacac
    57061 cagctgtaga aaatatggat tatggtgcag cttctagtct ctggaaaaaa gccagtgaac
    57121 gtttctacaa cttgggaaat cctgacaaat tctttgctga tcgagatgca agcgaatggg
    57181 tcagctttac cctgacgacg aaaaaccatt tgttgttgaa tgaaatcacc cgtatcacga
    57241 ctcaagaacc aggaaatgcc ttgaattcat ttatttcaac cacaccgatc acgccgttag
    57301 gccaaaaaga tgtcaatatg tctattgtcg tgcatcatca accgcatttt acgtctcaga
    57361 aaccaaatga aacagttatt tggggttatt tcctttatcc aagacgacgt ggcgaatttg
    57421 tggataaaca gtatattaaa atgaatggaa aagaaatgct agaagaactg attggacaac
    57481 tttcaaaagt agatcctgga ccagtgaata ttcgtgaaaa agaaacagaa attttcgaca
    57541 gcgtaataaa taatatccct gtctatatgc cttatgcttc cgcgttgttc aataatcgtg
    57601 ccaaaagcga tcgaccaaaa gtcattccta aacattcgac caatctggcc tttacaggag
    57661 aatttgtcga gcagccttat cagatgatct ttactgaaca aagtgctgta cgttctggag
    57721 aaatcgctgc tttccatttt gcgggtattc ctatgtcccg tcttgtcaaa acaccgcgtt
    57781 acgacaaaga tattcctact ctagctcgtg ccgcaaaaaa aatgtttgaa taatcaagag
    57841 gttgtgaaaa agctgccctg catcaagtaa taagaacaac caggctaaaa cagcttctta
    57901 tgtttttgat ttggtt
//
LOCUS       DACDXE010000008        57914 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.008, whole genome shotgun sequence.
ACCESSION   DACDXE010000008 DACDXE010000000
VERSION     DACDXE010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57914
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.008"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            273..839
                     /gene="amaP"
                     /locus_tag="IWM23_000471"
     CDS             273..839
                     /gene="amaP"
                     /locus_tag="IWM23_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9883144.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            852..1040
                     /locus_tag="IWM23_000472"
     CDS             852..1040
                     /locus_tag="IWM23_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9883145.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1053..1610
                     /locus_tag="IWM23_000473"
     CDS             1053..1610
                     /locus_tag="IWM23_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9883146.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1642..1884
                     /locus_tag="IWM23_000474"
     CDS             1642..1884
                     /locus_tag="IWM23_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9883147.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            2225..3439
                     /locus_tag="IWM23_000475"
     CDS             2225..3439
                     /locus_tag="IWM23_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAP9883148.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            3470..4897
                     /locus_tag="IWM23_000476"
     CDS             3470..4897
                     /locus_tag="IWM23_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883149.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            4920..5846
                     /gene="glsA"
                     /locus_tag="IWM23_000477"
     CDS             4920..5846
                     /gene="glsA"
                     /locus_tag="IWM23_000477"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAP9883150.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            6054..7514
                     /locus_tag="IWM23_000478"
     CDS             6054..7514
                     /locus_tag="IWM23_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883151.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            7735..9498
                     /locus_tag="IWM23_000479"
     CDS             7735..9498
                     /locus_tag="IWM23_000479"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAP9883152.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            9582..10040
                     /locus_tag="IWM23_000480"
     CDS             9582..10040
                     /locus_tag="IWM23_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAP9883153.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            10149..10832
                     /locus_tag="IWM23_000481"
     CDS             10149..10832
                     /locus_tag="IWM23_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9883154.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            10979..11593
                     /locus_tag="IWM23_000482"
     CDS             10979..11593
                     /locus_tag="IWM23_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAP9883155.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            11609..11815
                     /locus_tag="IWM23_000483"
     CDS             11609..11815
                     /locus_tag="IWM23_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883156.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            12354..12911
                     /locus_tag="IWM23_000484"
                     /pseudo
     CDS             12354..12911
                     /locus_tag="IWM23_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
     gene            12969..15008
                     /locus_tag="IWM23_000485"
     CDS             12969..15008
                     /locus_tag="IWM23_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAP9883157.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(15117..16268)
                     /locus_tag="IWM23_000486"
     CDS             complement(15117..16268)
                     /locus_tag="IWM23_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9883158.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(16422..16952)
                     /locus_tag="IWM23_000487"
     CDS             complement(16422..16952)
                     /locus_tag="IWM23_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883159.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(17051..17401)
                     /locus_tag="IWM23_000488"
     CDS             complement(17051..17401)
                     /locus_tag="IWM23_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAP9883160.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWM23_000489"
     CDS             complement(17558..19087)
                     /gene="ahpF"
                     /locus_tag="IWM23_000489"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAP9883161.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWM23_000490"
     CDS             complement(19097..19660)
                     /gene="ahpC"
                     /locus_tag="IWM23_000490"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAP9883162.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(19804..20466)
                     /locus_tag="IWM23_000491"
     CDS             complement(19804..20466)
                     /locus_tag="IWM23_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9883163.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            20650..22296
                     /locus_tag="IWM23_000492"
     CDS             20650..22296
                     /locus_tag="IWM23_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9883164.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            22287..23369
                     /gene="uxuA"
                     /locus_tag="IWM23_000493"
     CDS             22287..23369
                     /gene="uxuA"
                     /locus_tag="IWM23_000493"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9883165.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(23477..25030)
                     /locus_tag="IWM23_000494"
                     /pseudo
     CDS             complement(23477..25030)
                     /locus_tag="IWM23_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            25178..26319
                     /locus_tag="IWM23_000495"
                     /pseudo
     CDS             25178..26319
                     /locus_tag="IWM23_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(26445..27524)
                     /locus_tag="IWM23_000496"
     CDS             complement(26445..27524)
                     /locus_tag="IWM23_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9883166.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(27627..28067)
                     /locus_tag="IWM23_000497"
     CDS             complement(27627..28067)
                     /locus_tag="IWM23_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9883167.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(28124..28534)
                     /locus_tag="IWM23_000498"
     CDS             complement(28124..28534)
                     /locus_tag="IWM23_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9883168.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(28714..29148)
                     /locus_tag="IWM23_000499"
     CDS             complement(28714..29148)
                     /locus_tag="IWM23_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9883169.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(29248..30096)
                     /locus_tag="IWM23_000500"
     CDS             complement(29248..30096)
                     /locus_tag="IWM23_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9883170.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            30485..32251
                     /locus_tag="IWM23_000501"
     CDS             30485..32251
                     /locus_tag="IWM23_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAP9883171.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            32353..33612
                     /locus_tag="IWM23_000502"
     CDS             32353..33612
                     /locus_tag="IWM23_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9883172.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            33759..35060
                     /locus_tag="IWM23_000503"
     CDS             33759..35060
                     /locus_tag="IWM23_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883173.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            35063..35914
                     /locus_tag="IWM23_000504"
     CDS             35063..35914
                     /locus_tag="IWM23_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883174.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            35927..36742
                     /locus_tag="IWM23_000505"
     CDS             35927..36742
                     /locus_tag="IWM23_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAP9883175.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            36893..38059
                     /locus_tag="IWM23_000506"
     CDS             36893..38059
                     /locus_tag="IWM23_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9883176.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            38289..38936
                     /locus_tag="IWM23_000507"
     CDS             38289..38936
                     /locus_tag="IWM23_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAP9883177.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            39020..39964
                     /locus_tag="IWM23_000508"
     CDS             39020..39964
                     /locus_tag="IWM23_000508"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAP9883178.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            40099..41550
                     /locus_tag="IWM23_000509"
     CDS             40099..41550
                     /locus_tag="IWM23_000509"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAP9883179.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            41571..42407
                     /locus_tag="IWM23_000510"
     CDS             41571..42407
                     /locus_tag="IWM23_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9883180.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            42407..43066
                     /locus_tag="IWM23_000511"
     CDS             42407..43066
                     /locus_tag="IWM23_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9883181.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            43192..43881
                     /locus_tag="IWM23_000512"
     CDS             43192..43881
                     /locus_tag="IWM23_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883182.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            43887..44843
                     /locus_tag="IWM23_000513"
     CDS             43887..44843
                     /locus_tag="IWM23_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAP9883183.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            44887..45399
                     /locus_tag="IWM23_000514"
     CDS             44887..45399
                     /locus_tag="IWM23_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883184.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(45655..46203)
                     /locus_tag="IWM23_000515"
     CDS             complement(45655..46203)
                     /locus_tag="IWM23_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9883185.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(46292..47473)
                     /locus_tag="IWM23_000516"
     CDS             complement(46292..47473)
                     /locus_tag="IWM23_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAP9883186.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(47709..49217)
                     /locus_tag="IWM23_000517"
     CDS             complement(47709..49217)
                     /locus_tag="IWM23_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAP9883187.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            49376..49567
                     /locus_tag="IWM23_000518"
     CDS             49376..49567
                     /locus_tag="IWM23_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883188.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            49770..50687
                     /locus_tag="IWM23_000519"
     CDS             49770..50687
                     /locus_tag="IWM23_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAP9883189.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            50680..51753
                     /locus_tag="IWM23_000520"
     CDS             50680..51753
                     /locus_tag="IWM23_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883190.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            51901..52419
                     /locus_tag="IWM23_000521"
     CDS             51901..52419
                     /locus_tag="IWM23_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883191.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            52400..52792
                     /locus_tag="IWM23_000522"
     CDS             52400..52792
                     /locus_tag="IWM23_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAP9883192.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            52868..53803
                     /locus_tag="IWM23_000523"
     CDS             52868..53803
                     /locus_tag="IWM23_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAP9883193.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            53936..54715
                     /locus_tag="IWM23_000524"
     CDS             53936..54715
                     /locus_tag="IWM23_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAP9883194.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            54708..55025
                     /locus_tag="IWM23_000525"
     CDS             54708..55025
                     /locus_tag="IWM23_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9883195.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            55269..56879
                     /locus_tag="IWM23_000526"
     CDS             55269..56879
                     /locus_tag="IWM23_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9883196.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            57036..57884
                     /locus_tag="IWM23_000527"
     CDS             57036..57884
                     /locus_tag="IWM23_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9883197.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        19268 a        10230 c        11305 g        17111 t
ORIGIN      
        1 aataaataga atcttgtaat aataactgag cataatcttt gcaattttta atcttgaata
       61 atatccttta agtatagcca gcaatcaaaa gtaaatagag aataagtaag caattgttat
      121 ttcagtatca gactggcaat aaaacgccgt tatgatccag caatgtttgg tagcgcttta
      181 tttactgatg ctatgctaac tgttcgctat ctatactgtg attgtcggta gagttattta
      241 cagggtaaaa ataagtttat aggaggtgga aaatgaataa ggggttaaaa ataatcggtg
      301 ttatcatgag tttactgcta ctttcagtat ttctagcgat tgcgttgatc aatgcgccgt
      361 atattatgcc tgaacaattg gaacgatttc gcttctttac tttaacgaat tactacatgc
      421 aacaatatat tttctgggca gctgtagtat ttgccgttct tgctattatc atcttgctag
      481 tcgttctttt ctatccaaag tcaagaggaa ctttcgttat gaaacgggaa gatggcaaac
      541 taacaatcga caaaaaagcg atcgaagggt tggtacgttc ccatttacac gaagaagagt
      601 tcattcattc acctaaagta cggatacgtt caacgaaaaa tcgaatccat atccatgtca
      661 acggagactt gaaacgaact tcttcattag tagggaaaac aggtgtattg atgcaagata
      721 tcgaagaaga agtaactaaa gtactcggaa caaaagaaac ggtcaaagtg gcagtcacat
      781 acagcggtta tgaagaacaa gaagatacaa gagactacaa gcactctcga gtggaatagg
      841 agggccaaat aatgaaagaa ctattttcag catacaagct ccctattatc tttgggggtc
      901 ttggactact gctagctgtt ttatggttaa cgattggatt tttcaagaca ttgttgatca
      961 taatcatgac agcaattggc gcaggaatcg gtttttattt gaaacagtca ggaatagttg
     1021 atcaattttt taaatcatag gaggaaacaa agatggaaaa caaaaacatg aatacaacaa
     1081 atggatcagt tttggcaaat gaaaaaggag aaaaccgcat gatgaatggt gaattgactt
     1141 atgaagacaa agtcatccaa aaaatcatcg gttatgcatt gaaagatgta gatggtctat
     1201 tgacattaga tggcggattt ttctcaaata tggctgaaaa attagtcaac acagataacg
     1261 taacagcagg tatcaataca gaagtaggta agaaacaagt agctgtggat ctagatgtga
     1321 tcgtagaata cggtaaagac atcgaagata tttacaacca aatcaaagaa ttgatcagta
     1381 ctgaagtaaa caatatgaca catttagatg ttatcgaagt gaatgtgaat gttgcagaca
     1441 tcaaaacaca agaagaatac caaaaagatc aagaaacagt acaagacaaa gtaacagaag
     1501 cagcaaaatc cacaggacaa tttgcttcac gccaaacaga taaagcaaaa tctgctgtag
     1561 gcaaaggtgc acaaaaagtc aaagaaaata acgaaccacg tgtacaataa gaagtaagaa
     1621 gtcagttagg aggaatttaa aatgggattt atttgggcat taatcgtcgg cggtatcatt
     1681 ggagcaattg caggagcaat cacgaataaa ggatcatcaa tggggatcat cgcaaacatc
     1741 attgctggtt tagtaggttc agcaattgga caagctttac taggaacttg gggaccaaca
     1801 attgcaggaa tggctatcat tccatcattg atcggtgcaa tcattttagt attagtcgtt
     1861 tcattctttg cacgtaaatt ataagcaaat tgaagaaaaa cttatgttga aatgaagaac
     1921 aaatttgaga accgtagcga cactcgctgc ggttcttttt tatatatttt atatgaagta
     1981 ttctttattc cttatgaaaa aatatcttta aaaataaaaa aagacgaaaa taaacggttt
     2041 tctataaaaa gaagaccgtt tattttttgt ctttatttgt gtgaaaaacg aaaaagagca
     2101 caacacatca agcttttatt aattgtctgt cttttataaa tgcaagtatt aattcaaaaa
     2161 aacagaagaa aaaaacaact acaccttctt ttatactcgt aatcgtagaa aggaggcgaa
     2221 aaaaatgaat acattaccta tcaatacagg agatacagca ttcatgattt tatgtacagc
     2281 aatggtttgc ctgatgactc ctggtctagc ttttttttac ggcggattag cacggaagaa
     2341 taatatacta acgatcatgt ctcaatcttt tctctccgtt gggataacaa cgattatgtg
     2401 ggtatttggt ggattcggat tggcctttgg acgggatatc ggcggtgtta ttggaaaccc
     2461 ggcagatttc tttttgatga gacatgttac aagcttcccc aacatgtttc atggtgcaac
     2521 gattccattt ttaatgttct ttctttacca actgatgttt atcgtgatta ccgttccttt
     2581 gatgacagga gcttttgcag ggagattgaa tttaaaaggc tatattatac ttgttatttt
     2641 ttggaatctg ctgatttatt tccctgtttg ccactggata tggggtggcg gatttttgga
     2701 tcagatggga tttagagatt tcgctggtgg agcagtcatc cacacgacag ctggattcgg
     2761 ctctttagca tgtatcctca cacttggtca aagaaagatt cctttaggag aaagaagtca
     2821 gcatagcagt cttatggcag ctgctatcgg tacaggactg ctttggtttg gctggttcgg
     2881 ttttaattcc ggtggtgcac ttcgagcaga tgaacaagca gtcaatgcat ttggttcaac
     2941 attcatcgct cttgcatttg cgatgattac ttggctgatc attgcaaaag taaaaggaaa
     3001 tggctttgat tttgtcgacg tactaacagg atccgttgct ggattggcag ccattacgcc
     3061 ttgtgccggt tatgtagaag caaaaagtgc catgctgatt gggatcattg cgggtatcgt
     3121 ctgtcatgct gctgttgatt ttcgtaaaaa gaaacaatgg gatgatgcac ttgatgtgtg
     3181 gggtgttcat ggcatgggcg gattcacagg tacgattctg atagggatct ttgcatctaa
     3241 cagccattta ctaacgaatc caagcagctg gtacttctta gggattcaag tgattggtgt
     3301 catcattact gccgtgtatg cctatgtgct gacaacagtg attttaaaat cggcgatgca
     3361 ctttacaacg atcactacta caaaagaaga acaagaagaa ggattggact ttttcttctt
     3421 tggcaacaaa ctcaattgaa acaaattcaa atagaaaaga ggaagattca tgcaacaaag
     3481 caaaacgaaa attcctgcaa aaaaattaac gttctttggc tttttggcta tgaccatctc
     3541 aatggtcgtt tcattatatg aatatcccac attcgcgacc tcaggcttca gcttagtatt
     3601 tttcttgtta ttaggcggtt tgctatggtt tatccctgtg gcattgtgcg cagcagagat
     3661 ggcaacggtg aaaggatggg aaaaaggcgg tgtctatact tgggtcagtc gcacattggg
     3721 aaaacgattc ggttttgccg caatcttttt ccaatggttt gagataacag ttggctactt
     3781 gaccatgctg tatttcttaa ctggtgcatt atcctatgca acgggtattg gtgctataca
     3841 aaacaataaa tatctgaaat tggcaatcct attgattatt ttctgggcta ttttactctc
     3901 acagttaaaa ggaaccaaat acacttcatt gattgctcgg atcggttttg tagctggtat
     3961 cttgttgcca gcattagtct tgtttggctt agggatccat tatgtcgctt caggcgctcc
     4021 actacaaacg atactgtcta tgaaaacatt gattcctgat ttttcgaaat tgcctacgct
     4081 ggttgttttt gtttccttta ttttagcata tatgggcgta gaaacatctg ccagtcatgc
     4141 aaatgaaatg gaaaacccta aaaaggatta tccattagca atgtttgtat tggttattct
     4201 ggcaatcatt ttagatactt ttggcggatt gaccgtcgct actacagtac ctcaacatgg
     4261 tttgtctctt aatacagggg tgattcaatc gttagaattc ctattaagac acattgatcc
     4321 aaatcttgca tggattgcga aaattcttgc gattcttgtt tgtttaggtg tcatcggaga
     4381 aattgcttct tgggtaacct ctccttcaaa agcacttcac gtagcagcag aagaaggatt
     4441 acttcctgaa tattttgcaa aagaaaacac acacggtgtt cctgctcatt tgatgatcgc
     4501 aaatgggatc gttgcaacaa tctgggcagc cgtctttaca ttaagcggcg gggggaataa
     4561 tctttcattt cttgcagcga tgtcattgac tgttgtaatc tacctgatgt tgtactttat
     4621 gtttttcgcg ggctatttga agctggtttt taaagatgac agtcttgaaa gagcctatca
     4681 ggttccaggc ggaaaagttg taaaagtaat cgttgcttgt ttaggtcttt tgacttcact
     4741 ttttgctttt tgtatctcat ttgttccacc tgctagtatc gcagctggac agcatgggat
     4801 ttatgaaacg attctgattg tttcttatct tatcgtctta gttattccat ttacgatcta
     4861 cgcatgccgt cataaattcc agcatgcaga aaaataaaaa atgaaagctg gttattcaaa
     4921 tggaagtaaa agaattagaa caattgattg gaaaaaattt ccactattat caaacaggac
     4981 aagtggcgga ttacattcca gcattagctg aagtggatcc caaacaatta ggtatggcga
     5041 tttatgattt ggatacaaaa caactgattg agggaggaga cgccgatgtg cgctttgcca
     5101 tcgaaagcat gtcaaaagtt cccgtattgt tattagcaat caaagacaac ggaatagaaa
     5161 aagtctttca aacaatcaac acggaaccaa ccggttttgc cttcaattct atcatgaata
     5221 tggaaatcaa tcatcgcaaa catccaatga atccttttgt taatgcagga gcgattgcaa
     5281 caacctcgtt gattgccggt aaagatgcag atgaaaaatt tgagcgaatc ttgtcattta
     5341 tgaaagagat ctgtgatgat cctaatatca cattgaatga agaaatctat cattctgaat
     5401 caagaacagg tgatatcaat cgctctcttg cttactatat gaaaggtaat caaatgatcg
     5461 aaggagacgt accggagata ctcgatgctt atttcagaca atgctccctc aatgtcaccg
     5521 ctacaggcat agcaaaatta gctgctgtgc tggcaaataa aggaattgct ccttggaatg
     5581 gcaaacaaat cattactgaa gaaagtgcta caatcgtcaa atcaattatg acaactgctg
     5641 gtttgtatga tgaatcagga gagttttccg tccatgttgg tgtacctgca aaaagcggtg
     5701 taggcggcgg gttgatggca gctgtgccaa atcgttacgg cataggtgtc ttcagcccgg
     5761 cactagatcc atttggtaac agcgcagctg gtatccaatt gttgaaagat gtagtcaaag
     5821 aactagacgc tgacatcttt gaataaccat tcttgaaaga ggctggggta aaagtccaaa
     5881 cggctcaaag ctacctacaa tgaggctgaa attgtgagct tgtttgactt ttgtcccagt
     5941 ttcttttttt attggttttt gaaagggctt ttctaaataa aaaggaaaca aaaatacgat
     6001 ttacagatat gattaaataa aatcataaga aaaactggag gcgtgtatca ggaatgctgg
     6061 aaaattacat cgagcgaaat atatttagaa aagtgtatct gtgtgaacaa ttatttgaat
     6121 tccaagaaat agacatcgaa caaacagcta tttctctaag ggtgactaca ccaacgatcc
     6181 ttcacgatct agaaagtcta gccgagtgtc tagaatattg tataaaagaa caagtaagag
     6241 agaagcataa gtataaactc gtattcaaac atggtattgc tctttctgag ctcacacagt
     6301 ttctgtacgg tcaatcttat tttttaaagt ttttaagtta ttattttaat ggtatattca
     6361 cgagtacaga actggctgat cttgaattta tttctttgag caaagtatat acaatcaaaa
     6421 aaatcgtatt ggattttttt aaagaaaatg attatttgaa aaataaagaa atcattatcc
     6481 cagaatttga ctcgcggaac atcttgctag ctttagtacg ttatgtcaat tgggaaggct
     6541 atgaaaataa aaatccgtat gtagaagaag cttgtaacga gttgattgat tatgtagaga
     6601 ctcatttttt taaaagaaga tattcggaag atgaaaagtc ctttatcata cgaggaatag
     6661 agatcgcaat cggaagaaag gcatttcccc ttggactgaa agaagaagat aagaaaagcg
     6721 cagaaacgaa gccgttattt caattagtct gtcaaggatt ggaagagcaa aaaggaattc
     6781 tagacttaca agaagaagat gtctattata ttttttcttt gttcaacagt agaaattata
     6841 ccaatgagaa catggagctg ttaagaaaag atttcgaagt tgtttataaa aattttgttc
     6901 tcaatgacct atcactgaat gaattgattt tacagattat tagtatcgct gaaatcgacg
     6961 atgcagaaaa ttttattttc aagcaatcct ttttacaatt tgtccgtacg ttgtgggcag
     7021 atggacaagt atttttaccg gaaaggatct atcttctatc agaaaaagaa caacttctgt
     7081 acggaaaaat tgtagaaata ttggaagagt ggaaaaagaa aaatcaaatc caactaagat
     7141 ggaatgaaaa tttcattcga aaattcgtca aaagtctttc tttgatcaac agtgaacaag
     7201 cggggagaca ggaaatagaa atttttgttg tttcagaaag ttcagtcaaa caattttttt
     7261 atcgaaccca atttcatttg tatgcggaag aaggcatcgc tgaaataaat cctattttat
     7321 ttcgttcatt agaagaattg cctgatgaat gtctatgtgc gaaaaaaaga gtggttttgt
     7381 gcgatgctgc tagctatcaa aatggattag aaactgaaaa gacaaagatc tatccaatct
     7441 cattaaaagg tgcaaacatc catttgtatc atattgctca ggaacttcac ctattaaaaa
     7501 aacatatttc ttgatgagac cttaattagg agaccttcta caaagctttt taattgtctg
     7561 tagaaggttt ttatttgagt cctgattcgt gcgaagatct acttatataa caagctttct
     7621 ctgaaatttt tttaaaatgc tattgactta gagtcaactc taagtagtag aatagataaa
     7681 gtgtaacaaa tctattttca ttaaaagaag atagtacgga ggagagaatg tattatgagt
     7741 acagtattac atgtattgga tgcaatcaat agccccgcag atgtcaaaaa attatctgtt
     7801 gaggaaatga aacagttggc agaagagatg cgccaaaaaa tcttgcaaaa ggacagcgat
     7861 gtaggtgggc acgttggtcc gaatctaggt gtagtagaat taacgattgc gtttcattat
     7921 gtatttgatt caccaaagga taaaatcgtt tgggatgttt cccatcagtc ttatggtcat
     7981 aagcttttaa ctggtcgaaa aaatggtttt gaaaaaggac attaccgtga agtgagcgga
     8041 tattccaacc aacatgaaag taaacatgac tattttacag tggggcacac atctacatct
     8101 atcagcttag caacaggttt agctaaagcg agagatctga agaaagaatc aggaaatgtg
     8161 gtagcattta tcggtgacgg ctccctaagt ggtggcttag cttttgaagg attgaacaat
     8221 ggtggtgttt tacaatccaa tctaatcgta ttagtaaacg ataatgagat gtctattgat
     8281 gagaatcatg gcagtttata taaaaactta gctgagcttc gtcaaaccaa cggtaaaagc
     8341 agcaataacc tatttaaagc aatgggattt gattatcgtt acgtggataa tggaaacgat
     8401 ctggaaacgg tgatccgttt atttgaagaa gtaaaagata ttgatcatcc gattgtactt
     8461 cacgtccata cggaaaaagg tcgcggttat cagccggcaa tcgataataa aatgaaatat
     8521 cattggcatg taccatttga tctgtccacc ggggaggcaa agcaagtaac ttctggagaa
     8581 acatttaata tgatcatcat ggattatttg gataagaaaa tgcaaacaag tacagctcct
     8641 attgccgcag tcaatgcagc gataccaggc gcctttggac tgaaaacatt ccaagaaaaa
     8701 catccagatc attattttga cgtggggatc gctgaacagc actctatcac tttcgcttct
     8761 gctttggctt ctcagggagt acgtccagtc gtcttccaat caagtacttt cttgcaacgg
     8821 gcatatgatc aattatcaca tgatttagcg atcaacgaga atccagtgat tattttagtc
     8881 ggtggcggaa cgatctcagc tagtgaccga acacaccaag gaacatttga cattccttac
     8941 ttatcaagta tcccgaacat caaatatctt gctccagcaa caaaagagga tatgatctct
     9001 atgctagatt gggcgttcga acaaacagcg cagcctgtag caatccgaat tcctacacat
     9061 ggtgtagaac atggtacagc tatcgcaaat gattatagta ttccagccta tcaaacagta
     9121 aataatggag gaaaagtagc aattttagct ttaggcggtt ttcttgaatt aggaaaagaa
     9181 acagtaaaag cttttagaaa aaaaggaatc gaagcaacgt tgatcaatcc aggaacgatc
     9241 acagaaaatg acgtagaaac attagataaa ttaaaagaaa cacacgaatt agtcatcaca
     9301 ttagaagatg gtagtttgtc aggaggattt ggcgaaaata tcagtcgtta ttatggtgat
     9361 tcttcaatga acgtattaaa ttttggtgca gagaaagaat tcacggatag cgttcctgta
     9421 gggcagcttt atgaacgtta tcatttaaca ccaaaacaaa tcgttgaaga tagtctgcgt
     9481 gtcttgaata ttcaataatt cattatgctc accatccaga taattgttta tactagtatc
     9541 tggatggttt tttcattata aaaagcaaag gggtggatgt tatgtatcag caagtagaag
     9601 aatatgtcaa aaagttccaa gaaaaatatc ctagttatca atcagaaaaa ttaggtttct
     9661 tttcatttgg ggatacagaa aaaatggcag acgagttgag cgaactagtt gtaaaaaaga
     9721 taaagactgg tacatcaagt ggttacgatc tgtataaaca agagaatgag cctttaccaa
     9781 aagttggtca attagacgtg gtcctgaatg gaaaaaatga accagtttgt atcatcaaaa
     9841 atataaatgt gaccatcttg ccttttagtg aagtcacgaa agagcatgct tggaaagaag
     9901 gagaagggga tcggacatta gcttattggc ggaaagtcca tgctgagttt tttaaacatg
     9961 cttataaaga agcgttgaac atagaattta cagaagagaa gctggtcgta tacgaagaat
    10021 ttgaagtaat tttcttttag taagaaaggt ctgctgaaag aaatagctca ttattgtctc
    10081 ctgaatagaa aagggttaca tttttatatg aagctgtatt tttatataac attactggag
    10141 gagatattgt ggatacactg gaacaaatca aaggcggagt cgtcgtttct tgtcaagctt
    10201 tggaaaacga gccgctacac agttctttta ttatggggag aatggctttg gctgcaaaag
    10261 aaggcggagc agttggtatt cgagcaaata cgacagaaga tatcaatgaa atcaagcgaa
    10321 tcgttgattt accggtaata gggattatca aaagagatta cgatgggtca gaagtgttta
    10381 ttactgcgac agaaaaagaa gtggaagaac ttttggagac gcaagcagaa atcattgcac
    10441 tggatgcaac ttctcgtaaa cgtccgacag aaaaaacaac tgctgaactt gtccaaatga
    10501 ttcacgaaca cggtcggtta gctatggcag atatttcgac ttttgaggaa gcgcttcaag
    10561 cgcaagaaga tggttttgat cttttgtcta cgactttagc aggctataca gaatattcac
    10621 gaaagacgca gacacctgat tttgatttat tgaatcaaat catagaacaa gtcgatatcc
    10681 cagtaattat ggaaggacat actgactctc ctgaacaagt agagaaagca tatgaattag
    10741 gtgctttttc agtcgtagtt ggatctatta tcacccgtcc gcaattgatc actaaaagat
    10801 atgtcgaagc cgcaagtaaa gcaaataact aaaaaacacg cacggcacag attcttgaca
    10861 aaaaagaaaa attgatagat agtttaaagc agattctaag aagatgaaaa aagcattact
    10921 tttcattttg tcttcaagat ctgcttttta acgaatttta tagaaagaag gcgaaaccat
    10981 gtttgaacgg agtccttcag aattgacaga acgtgaaaat tacaagttgc tgattggttc
    11041 agtcatccct agaccagtag cagtagtaac gactaaatca gcaaaagata tagtgaatat
    11101 cgcacccttc agttacttca atatcgtcag ttcgaatcga cccatcttat ccttggcgat
    11161 ccagagaaaa caagatgcag taaaagacac agcaaagaat attcttgaga caagagaagc
    11221 tgttatccat atccttgatg aggacaacgt agaagaagta aataaaacag cagccaatct
    11281 gcctaatgag gaaagcgaac tgtacctgac tgacttgact ttaactgatt cagtaatcat
    11341 ttcagtacca gcattgacag aagcaaaagt tcgttttgaa acatccttat atgaacatgt
    11401 cgagatacgg aataaaaata aagtaacagc tgatttattt ttgctagaag tacagaaata
    11461 tcatttaaat gaaacagtct atgatgataa gacaggaagg atcgatccaa gaaaattgaa
    11521 tgctgtaagc cgtttggcag gaaatgatta tgcagcaata ggagaaattt tcacgattgc
    11581 acgtcccgaa taaaaaggag cactagctat gttaaaaata agctataaac catcgacaga
    11641 ttcaaaagaa atgaaaaaag aatatgaaac agtgaatgat tttctgcaag ggcagtactt
    11701 agaagttccg ccattgcaag accattttgt cgttactact gttactttag atggtaagga
    11761 aatagaaatg ccagatcaaa cgattagcgg attgtttaat tactttaaca aataaatatt
    11821 tattccgaaa agtgaaaaaa catataaaaa aacacataaa actaaaaaat tatcaaagaa
    11881 tcctggtgca actgaaagaa tttgcaacgg gattctttca acttatttgt tatttttttc
    11941 taaaaaatat taaagattgt taaatttcac tatttcattt caaattccat tgcctaatca
    12001 ttaggaaaag tctatcatga actcgtaaat agaatatagt aataaccttg ttaggtgagg
    12061 ctcctataca aacataggct gttgcccaga aacgtcgaaa gtcgccaatg ggtaaaacag
    12121 aaactgttgg attaaggctt tttccaatag ctaagaatgt tttctttacg ttgtatagtg
    12181 ctaaaactcg gacgatgaga tgatgctagg tcagaattct tgttcgaaaa ggtttctgac
    12241 cttttcttta tatttttgtg actgacttat ttgatgagac aaactcgctc ctcacctgac
    12301 aagtggattg ctgtgtgagg aggagatgcg gtgaaaaaag atcgattggt ggaggtcgct
    12361 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
    12421 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
    12481 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
    12541 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
    12601 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
    12661 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
    12721 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
    12781 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
    12841 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
    12901 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
    12961 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
    13021 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
    13081 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
    13141 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
    13201 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
    13261 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
    13321 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
    13381 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
    13441 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
    13501 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
    13561 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
    13621 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
    13681 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
    13741 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
    13801 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
    13861 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
    13921 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
    13981 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaata gcttttgaca
    14041 gtctaggagg aaaagctgca tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
    14101 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
    14161 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
    14221 atgctcctgg atctacgttt aaaatgatta cagcagcaat cggtctcgac aacggcacta
    14281 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
    14341 gatcttttaa aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
    14401 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
    14461 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
    14521 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
    14581 gatatggaca gggcgaactt ctaattactc ctatccagca agcagcaatg tattctgttt
    14641 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
    14701 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
    14761 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
    14821 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
    14881 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
    14941 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
    15001 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
    15061 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
    15121 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
    15181 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
    15241 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
    15301 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
    15361 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
    15421 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
    15481 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
    15541 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
    15601 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
    15661 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
    15721 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
    15781 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
    15841 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
    15901 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
    15961 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
    16021 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
    16081 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
    16141 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
    16201 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
    16261 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
    16321 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
    16381 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
    16441 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
    16501 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
    16561 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
    16621 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
    16681 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
    16741 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
    16801 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
    16861 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
    16921 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
    16981 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
    17041 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
    17101 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
    17161 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
    17221 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
    17281 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
    17341 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
    17401 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
    17461 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
    17521 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
    17581 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
    17641 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
    17701 tcactatttc atttcttgga ttcatttcga tcgttctatc tagccaatcc gtattaggga
    17761 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
    17821 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
    17881 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
    17941 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
    18001 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
    18061 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
    18121 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
    18181 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
    18241 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
    18301 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
    18361 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
    18421 ctgcactact agctgctggt ccaccgccaa tcactaatac atcataaggt tctttttcaa
    18481 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
    18541 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
    18601 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
    18661 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
    18721 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
    18781 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
    18841 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
    18901 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
    18961 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
    19021 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
    19081 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
    19141 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
    19201 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
    19261 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
    19321 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
    19381 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
    19441 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
    19501 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
    19561 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
    19621 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
    19681 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
    19741 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
    19801 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
    19861 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
    19921 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
    19981 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
    20041 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
    20101 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
    20161 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
    20221 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
    20281 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
    20341 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
    20401 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
    20461 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
    20521 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
    20581 catagcgatt tttattgcat aaaattcact tgaatacaaa aactaatatg ttagtttagg
    20641 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
    20701 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
    20761 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
    20821 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
    20881 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
    20941 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
    21001 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
    21061 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
    21121 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
    21181 attcttgctg tgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
    21241 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
    21301 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
    21361 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
    21421 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
    21481 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
    21541 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
    21601 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
    21661 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
    21721 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
    21781 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
    21841 ccaaatattc ctaatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
    21901 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
    21961 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
    22021 ggaaaaccat tcatcccgag tccagaccca ctattgacag accttcagca taagctttcc
    22081 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
    22141 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    22201 gaaatgttca tcaagatggt agcaggacca aatgccgtac gaaaaacatt acaggattac
    22261 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    22321 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    22381 cattgatgga tataccagta ggagaggtat ggcctaggga gaggatcaaa gaactaaaag
    22441 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    22501 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    22561 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    22621 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    22681 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    22741 aatttggatt gccgggatgg gaacctgagc gcttagctcc tatcaaatcc ttgcttgaag
    22801 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    22861 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    22921 actcgatttt tggattagct cgaatcgtaa agaatcgaga agatttagat tggttgtgtc
    22981 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    23041 cagaaaatga tgtatatggg attttagcag aattttgcca aagagatcga atcccatttg
    23101 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccacataaat
    23161 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    23221 acggatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    23281 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    23341 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    23401 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    23461 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    23521 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    23581 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    23641 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    23701 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    23761 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    23821 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    23881 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaata
    23941 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    24001 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    24061 caggtataaa caaatcaaat acagtacaaa gggactccac catttctctt gaataaagaa
    24121 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    24181 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    24241 ttggcagatt aggtcgcaaa atatctacag agagatcaat aaaaaagtca atatattcat
    24301 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    24361 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    24421 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    24481 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    24541 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtactatg
    24601 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    24661 gctgactgaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    24721 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    24781 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    24841 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    24901 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    24961 gaaaaatatc taattttgta ttgctttttt tcttcaagat aaacgtttta atcgcttctt
    25021 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    25081 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    25141 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    25201 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    25261 gacagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    25321 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    25381 catcaaagca aaaattgact attcgggaga cttagcacct atactacaat cagctgttac
    25441 atttgagaat gttgatccca ctttgtcgat tggctatgac gagaacactt tgactatcca
    25501 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    25561 gctttttact gtcaaggtat cagatggact accgaacgat catcatttaa cgccgtatag
    25621 tcagcggcag acaattgagc aagattcagc catcgactca ggaggagatc ttgttgaaga
    25681 accaacagat aaaccagaaa atgaaaataa gccagaagta ccgcctacag aaaatccaga
    25741 cggagaacag aaaccggaaa tagaacctgg agaagaaccc gatacagaaa ctcaaccaga
    25801 gccagacaat aaatcaaaac ctgaaataac ccctggagaa aaaccagatg taactccaga
    25861 accagatacg gattctggaa accaaacagt accggaaaca aatccagaca cagataatga
    25921 aactgaaaat ccagaaaaac cagaggtaga tccagaagaa aaaccagatg tgactccaga
    25981 accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa aaacaattca
    26041 agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa taaatgaaga
    26101 gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa ccttaaaaag
    26161 tagcaaggat acaacctttg ctgtcctgaa acgaaaaata aacagctacc aaaatccgga
    26221 gagtcacaaa acaaagttat actgtggagc ggaatcattc tattaagtat agcaacaatg
    26281 ctttctgcaa aaagatttaa acaaaacaga tctctctaac atttgaataa tgtctgtgaa
    26341 cgcatttttc aaataaaaag aaatatccag aaaatgaccc caaaatacca gattgaaatc
    26401 tgatatttgg ggtcgttcat actttatctt tcttcatatt tattttaagg gatatctgtt
    26461 tcttttggaa gatggttcgg gatctttttc actttcaata catctaaaaa gaaggtaaag
    26521 atcggaatgc cgacaatcaa gccccaaacg ccaaagaacc gttcgctaac aagtaagacc
    26581 acaaatgtat aaaaaatcgg cagttctgtt ttgctggaca tcaacttcgg attcagcaca
    26641 taagactcaa ttagatggac aatcaaaatc gtaatcagga tataaacaac atctcggatg
    26701 cctccttggg aataagctat gaaagataga ggaatacaag aaatgataac acctgctact
    26761 gggatcagac ttaaaatgaa aatcatgatc gctaaactca gcagctgatg aaagccaaat
    26821 acagccaagg cgattgtagt gagcaacgta tttaaaagag cgacaacaaa ttgagcttct
    26881 aataccaaac caaatgtatt gacaaactta tcggcaaaat agtaaatatc ttgaaagaac
    26941 caagaaaatt cactttttaa aaacaatctt gagaatacaa ttgttttttt cttttcaatc
    27001 ataaaaaaga aacttaagat gaatgacata gcaaacgcca ctgccatttt tcctatatcc
    27061 tgtaaatagc caagcgcaat cgaagcgcca ttttgaagct gttcaaaaaa atcgtttcgc
    27121 tcaatataag tatggatata ttgtaaaaca ggatctgcat cttttggctg atgctcgtaa
    27181 aaattaataa cggactggat catgtcaaat gtttgatgaa taagcatcgg gatatacttt
    27241 gtgaatgcca gatagataag gtaaacgatc agtgtataaa tcatgatagc taaaacacct
    27301 gtagggattc ggacaaatcg ctgaatgatt tgaacaagat gaatcatcaa ataagtaaag
    27361 ataaatgtga gcaaaatggt tgtaatcata cttctaacta agaaaagaac aaaaacgaca
    27421 aataataaaa cagtgagacg gcgtattttt tcattcgcca aaaaacgctg ataaacggtc
    27481 aaatcgacaa cctccaaata acaaaaaata aacacgttat tcattatatg atggaatatg
    27541 aaaataagaa agagcaaaaa gaaaaaccgc taaatgacgt tatcttatga gttggttttc
    27601 ctcataaaat cgttcatcta gcagtttcag ccctttttct ggtcttgatg aaaattatct
    27661 agcatttttc gggtcagatg ttgcagtgta tccaattctt cctgttcaag tggaagataa
    27721 gacttgatct tttgtggaat accttctgct tgtttttgca acgcagcacc ttgagcagtc
    27781 agcgtaacat ttacgattct ttcatcaccc gtttcacgct gacgattaat ataatttttt
    27841 tctgctaact tttttagtaa gggagtcagc gtaccagaat ccagacacaa atcatttcct
    27901 agctctttca ctgaagtctt tttcttttcc cataaaacta acatcacaag atactgggta
    27961 taggtaagat tcagcggctt gaggagggga gtgtagtgtc taatgatttc tttagaggcg
    28021 gcatataaag gaaaacatac ttgattaact aaatatagat cttccatttt tccacctccg
    28081 ctgttttact atagtgtatc atagcggatt tacacaaaaa agtctattca ctaactgctt
    28141 tcagagtaac ttctatattt ccagctgtag cttttgaata cggacacact ttatgcgctg
    28201 cctctaataa ttcttgcgtt tcttccagag aaagaccttc gatagatcct tcaatctcta
    28261 cacctaagac aaagctcgtt tcactttctg aataaaggga aacttgtgca ctgacagtag
    28321 aagccccaga gatatttttt tgctgtttga tcaattctaa tgcactgtta aaacatgcac
    28381 tgaaaccagc tgcaaacaat tgttcagggt tggttgcatt ttcgacgcgt ttaccaggag
    28441 ctgcaatatt caattcgaat gtacgatcgg gattatgcac ttctccagag cgtccgcctg
    28501 tgttgatcat tttcgtagaa tacatttttt tcataaagtt accaccttta attatatttt
    28561 atacaattga attttacaca atataattgt ggaaagcaaa gaaatggatt ggcataagat
    28621 aaataagaga aagcacatga aacagacgcg ctttctcgaa tagcaaacat atacaaaaaa
    28681 tatcagagac aaaaaataat ctcatcataa acattacata tatttctcaa aaagttgttt
    28741 tctttcttca aaaattactt cacgatggac ctgatgaagc atccataaat aaccaaaagg
    28801 atctctgaac attgagttag tcacaccaaa gtccatcatt tctgtcattt cttgaatctc
    28861 tacacagcca tttcgtaatg ctttattatg tgtgtcctcg atatcagaga cgatgatatt
    28921 gaaccagatc gtatttggat gttcagcagt aggagcagtc aaaccaagag acgtgttttc
    28981 atccagcata tggaatctcg taccatacaa ggtaaaaatg acttcattat tgcctctagg
    29041 gtaatctgtt gcctcaatca gttgtatttc atcaaagatt gtttgataca gttctagtgc
    29101 tttcaaacta tcatttaata caaaatcgat ttccgttcct ttatgcataa agatctcctc
    29161 tccttttcag tcgaacagtt actagattct tttattatac cagctaaaga aaacgcattc
    29221 aaaaaaatag cggatttact taccaactca ttcgattgga tgataagtaa atccgcgtcc
    29281 cattacttct gctacgctgc tgacaatgac aaaggcactt ggatcgattt cttctatcaa
    29341 tggtttcatt cgggaaaatt catgatcagt cacaacgatc aataaaacct cttttgactt
    29401 tcgattatac ccgccttttg cctctagcag ggtaaggcca cgtccaatct cagcacttaa
    29461 gcgaaggcgg atgtcttcca aataatgttc actcatgacc ataacagatt tctttcgatt
    29521 gatacctgtt tcgatataag tcatgacaat tgaagtgatt gcaatagata aaatagcata
    29581 aagaaactct tctattccaa aaataaacag attaagagaa acaacgaatg cgtctgtaaa
    29641 aaataagcca atcgaagtat tcaagtggaa atatttttta aaaatgaggg gagggatcgt
    29701 cgtaccgccg ctagaagagt tgtttttata taaaatcgca actcctaaag caaaaatcgc
    29761 acttccaaaa atgacagaca acaaccgatc tgaagtaatc attgtttctg gaactattgc
    29821 aatgattaaa ggaaaaacca agcttccgaa aagtacttta aaaaagggtt ttttacctaa
    29881 aaatagaaga gctagtacca acataacgat attcaacact aaaataacta tagctcggtc
    29941 aaagcctaaa gcagcttcca gtaatattcc tagaccgctg acacctccag cagcaatatg
    30001 atgaggccct aaaaacatat tgatagcgac tcctaatatg aagagggcaa aaataatctt
    30061 aatgtatgtc catataaatt gtctcgtttc tttcatttat tcacttcctt aattcattaa
    30121 taatactctt atcttattat aacttacttt tcaccttaag ttaactttaa ttaataaaaa
    30181 aattaggcta gtctaaatta ttatgtttca ttcttctaga atatacttct acgccaaaaa
    30241 ctgttaaaaa acttttactt tagaaaattg gagaaattga tcgcttaaga taaattcagg
    30301 acactaaata gtacaggaag tagactaaaa cttttaaaag ttttaagtct atctatttgg
    30361 caagtttaaa aaataaaaat tcggtatcaa aaaactgtta ccgataacat ttttgaaagc
    30421 gttgtctttt gcttctctat cacttatagt gatgacgtca tattaaaaca caaggaggaa
    30481 atcaatggat acagctgcaa tttatcatcg accggaaagt gaatatgcat ttctatatac
    30541 gaatacaaga tttcgtattc ggcttcgtac taggaaaaat gatatcaaaa aagtatatgt
    30601 gctatgtgga gatccatata ccataacaac tgaaaaatgg tatcaaaaac aacgaccaat
    30661 gaaaaaatgt ctaagtacca atgttcatga ttattgggaa attgaagtga cgagcgaaac
    30721 aagtcgctta cagtatgcgt tccatgttgt tggggaagat aacacggact gtttttacgg
    30781 tgatcaagga atttttcctt atcgagaaaa tgtgattact gaaccaaatt tttattttcg
    30841 aataccttat tttcatcaaa tcgatcgatt cactattcca gaatgggtaa aagaaacagt
    30901 atggtatcaa atttttcccg aaagatttgc aaacggggac aaagcaaatg accccgaaca
    30961 cactttacct tggggaagta aagatccaga ccgagaagac tttttcgggg gagatttgca
    31021 aggtgtgatg gatcatttgg attacttgac ggaccttggc atcaatggca tctatttttg
    31081 tcccatattt aaagccacat ccaatcataa atacgatacg attgattatt atgaggtgga
    31141 tcccgctttt ggcgataaaa aacttttaaa aaacttgatt gatgaagcac ataaaagagg
    31201 gatacgtatc atgttcgatg ctgtattcaa tcatatgggt gtccattcac cacagtggca
    31261 agacgttctc aaaaacggag aaaaatcgat ctataaagac tggttccaca tccgtttttt
    31321 cccagtagat tcttatcaga tgactgactt accagaaact gctgaaaata tcccatatga
    31381 tacttttgct tttactccat tcatgccgaa actaaataca gcaaatccag aagtgcaaaa
    31441 atatttgctg gatatcgcaa cttattggat aaaagaattc gatattgatg gatggcgtct
    31501 tgatgttgct aatgaagtcg atcatcattt ttggaaaaaa ttcagagaag cagttacaga
    31561 aatcaaacca gacatctata tccttggaga gatttggcat tcctctcaag cttggctgca
    31621 aggggatgaa ttccatgcag tgatgaatta tgcatttaca gattcgatta aagattattt
    31681 tgcaaaaaag aaaatcacag cttcacaaat ggtcagtgga atgaatcatc agcaaatgct
    31741 ttacagagat caggtgaacg aaggaacatt caacttatta gattctcatg atactgccag
    31801 gatcttgacc ctttgccaag gtaacaaaga attgatgaag tctgtgatgg cctttatgtt
    31861 tcttcaaaaa gggtctcctt gtatctatta cgggacagaa atagggatga ctggtgaaga
    31921 tgacccggat tgccgcaaat gtatgatatg ggagaaggag gaacaagatc tagagctatt
    31981 cggttttata aaagaacttg tttctctaag gaaacagttg tctaagatta tttctgaagg
    32041 ttctactcag tggctaatag taaatgatcg tgaagataag ctctatttca cacggaaact
    32101 agagggacag atcatctatg tttactttaa tcagtcaaaa gaaccttggg tagtagaaca
    32161 ggaaaatgag gtgattctta gtcaaaattg tcagttgtta gaagacggga aagcagagat
    32221 acgacaatac ggatttttga tcatgtgttg aaaactgtta ccggtaacaa caagaaagcg
    32281 tttgcaaatg aagatttgtg cccattataa taagggtgta acagcacgtt gaatacaaag
    32341 gaggaaatga agatgaaaaa aagaagtgtg aaacagttgg gtttagggtt aatctcttta
    32401 ggaatcttag gcggattagc ggcttgtggc aatggtaact cttcagcaga aggggaagcc
    32461 gatgaaggaa aaacgttaac agtttccgtt gattctggat ataaagatta tataaacgaa
    32521 ataaaaaaca ctttcgaaaa agaaaacgat gtgaaaatca agctagtaga aaaggacatg
    32581 ttcgatcagt tggaatcatt agcgttagat gggcctgctg gaaagggacc tgacgttatg
    32641 atggcagcat atgaccggat cggtgcgtta ggacagcaag gacatctggc ggaagtaaaa
    32701 ctgggaaatg aagcagctta cgatgaaaca gacaaagccc aagtgactta tgatggaaaa
    32761 atctacggag aacctgcagt aatcgagacc cttgttttgt actataacaa agatttagtc
    32821 gatacagcac catcaacgtt taaagatttg gagaatctat ccaaagacag tcgttttgct
    32881 tttgagtcag aagcaggaaa aaatacagga tttttagcta aatggactga cttttattat
    32941 tcctatggtt taattgcagg ttacggtggc tatgtatttg gtgatgatgg cacaaatcct
    33001 tcagatatcg gtttgaataa tgcaggtgct gtagaaggaa tctcttatgc aacagattgg
    33061 ttcaaaaatg tttggccaaa gggaatgcaa gacataaaaa gtgcaggaga cttcgccagc
    33121 cagcaattca tgtccaataa aactgcagca atcattgacg gtccatggca agcgcaaaca
    33181 tacaaagaaa acaatatcaa ttatggtgta gccaaaattc ctactctaaa taatggacag
    33241 ccttatcaac cgtttggcgg aggaaaagga tgggtagtca gcaattatgc taaaaacaaa
    33301 gacctctctc aaaaatggct tgattatgta acgaatcagg aaaatcaaga aaaattcttt
    33361 gaaatggtaa atgaaatccc tgcaaaccaa caagctaggg aaacagcaaa ggctaaaaac
    33421 gatgaattaa caacagctgt gattgaacaa tatgaaacag ctcaagcgat gccaaacatc
    33481 ccagaaatgg gcgaagtttg gacaggtgca gaaaacttga tgttcgatgc agcatcaggt
    33541 tcaaaaacac caaaagaatc cgcagatgaa gcagtaaaaa caatcagtga agccatagaa
    33601 caaaaatact gattaaaatg ataaccgtcc atttttataa aaaatggaac aggtgcttca
    33661 gataaaagtg gcaagagcga gatggaaaat tatcttgctc ttgttgccat tatcttaaaa
    33721 gcagtttgcg gtttagcaag tttagagggg gcattcagat gcagcaagat aaaacaagca
    33781 atatcaacga aaaaaaaatc acgctttgtt cacttattcc agggatgggg cagtttgtaa
    33841 atggccaagc ttttaaaggt atcgtttttt tcgccatatt cgtcctattc attggtcaga
    33901 tgcttctggg cggatttcat gcattggtag gattggttac tttgggaagt ataccaatgg
    33961 aagatcattc attatttatt ttgattgaag gcactttgca attcatcatt acaatcattt
    34021 tcttgatttt ttacggtttg aatcttttag acgcttatcg agtagcgaag atgcgtcaag
    34081 agggcaaaaa aatcaatcgg acgatgaagg aagtcataca taatataggt gatgaaggtt
    34141 ttccttatct attgacactt cccgcttatc ttctcatgat ttttacgatc attttcccag
    34201 tcctagttac attgttcact gcatttacta attatgactt caatcatatt ccaccagcca
    34261 gtttaattga ttgggtaggg gctaaaaact tttttagtat cttcttttta agctcatatc
    34321 gtaatacttt ttttgcagtc ttttcgtgga ccgtgatttg gactttatgt gcaacaactt
    34381 tgcaaatcac ccttggtatt ctcacagcgg ttgttgctca ccagaaattt attaaattca
    34441 aacgtatttt cggtatcatt ttcttattac catgggcggt cccagctttt attactatca
    34501 tgagtttttc gaatatattc aacgatagcg taggtgccat caatactcaa gtgattcctt
    34561 tcataaacca gcttcctttt gtagatatca gtgcaatctc ttggaaaacg gacccgaact
    34621 ggacgaaagt agcaattatc ttagtgcagg gctggttagg atttccctat atctatgtga
    34681 tggttacagg aatcttacaa tcgatttcag aagacttata tgaagcagca aaaatcgacg
    34741 gagcaaatgc atggcagcgc ttttcaaaca tcacgcttcc tgctgtcttc ctagtggcag
    34801 cgccaacatt tattacacag tatacgggga atttcaataa tttctccatg atctatctgt
    34861 ttaacaatgg cggtcccgga agcgtaggcg gaggagctgg ttctactgat attcttattt
    34921 cctggatcta taaacttacg actggaactt ctccgcagta ttctatggct tcagcaatca
    34981 cgttgattat ctccgtcatc gttatctgtg tgtcattgct ggtatttaaa aagacaaatg
    35041 cgttcaatat gggggattaa caatgaaaaa atttcattcg agtgctcgat tccaaaaacg
    35101 actttctcac ttttttactt acttttttct tttaatactg tcagtcatta tcatctatcc
    35161 gcttttgatc acagcaagta ctgccttcaa gtcaggaaat attgcagctt ttagtttaga
    35221 tctttcaagt gactggactt taggaaactt tactcggcta ttcagtgaaa cactttacgg
    35281 gacttggtat aaaaatacat tgatcatttc tgtcctaaca atgatcgtac aagtgactgt
    35341 agtaactttg gcgggctata cttatagtcg ctatcgtttt atcggccgca aatcaagttt
    35401 gatgttcttt ttagtgattc agatggtacc gactatggca gcattgactg ccttttatgt
    35461 catggctttc ttattagaag cgctcgataa gtactggttc ttgacaatga tctatatagg
    35521 cggtggaatt ccaatgaata cttggctgat gaaaggatac tttgacactg taccaataga
    35581 tttagatgaa tctgctaaac ttgatggggc agggcatttc aggatttttt tccagattgt
    35641 attaccgctt gtcaaaccga tgctcgccgt tcaagcatta tgggctttta tgggaccttt
    35701 tggggatttc atgttagcta agttcctgct tcgaacacct gaaaatcaga ctgttgctgt
    35761 cggcttacaa acatttattt ctgatgtgac aaaccaaaga gtctctcttt ttgctgctgg
    35821 ggcaattttg attgcactac cgatttgtct attattcttc tttttacaaa agaactttgt
    35881 taccggcctg acatctggcg gaacaaaagg ataggagcag agtatcatgc aaacatttcc
    35941 gttaaactat ttttccgcat tacgtacgcc aactcaagtg tttgcgggac gcaaactatt
    36001 atcatggcct aaatttttct tgatatttgt atttttagtt tccttgatgg tcatgcctgt
    36061 gactcttttt tatgccaatc agatccaagc gattccatta gaacaatttc tatccgttca
    36121 ttcgctgatc gatgaacaag gaacccaaaa attttctgaa cttgaattat ctgaaactgg
    36181 gttacaagca tcgcaacaaa cgatagctgt gacccctgaa attttggtag gtgtgtcttt
    36241 atctgagaaa caacaatccg atcatggaac ttttattgat tttgagaagg aacaatgggt
    36301 gatccaacaa aaggataaat ctggtatacg ccgctatacg atgaattata gcccatcttt
    36361 ccaaccagat tctgtacgta cgcccgaaga tttccaacga tttttagaaa gagaatttta
    36421 tgccagtaat cgtccaacaa tcattttatc ttatagttta agtcttggtc ttgtcctatt
    36481 tgtcatgacg agtctcattt tattcggggc ttcctttttc ttgtggatga caagaaaaag
    36541 ccaactttca tccattcata cattcaaaga gagtgcaaat ttgatgctga atgttatggg
    36601 aataggttcc atggcagcag cagttgtagg gtttatccac tttgacttta tcttgatgct
    36661 aagtgtccaa acaacgataa ctgtcttgct gcttttatgg gtttttgtca aaacaagatt
    36721 caaagataag cgtgtcgaat gacaagttta agcgatttat gcgacaatga atgtgaaaaa
    36781 aatgaaaaat aaagtagttt ttgaaaaatt tctgaaaaaa ttaaacttct tagttgattc
    36841 ttttcttttt tagtattaac gtaatagggt agttttttta aggaggaaaa tcatgttaga
    36901 gaaaacttta tacaatcaat tattttcacg ttctttctcg ctccctgtcg aagtaaatta
    36961 ttgggacggt acaacgaaac aatacggaga tacggacact cctccccaaa tcaaaatcaa
    37021 tatccatgaa gaaatcccaa tgaaagagat tacaagtaac gcttctttgg cactaggaga
    37081 agcctatatg gatcatcgaa tcgagatcga gggaagtatc caagcattga tcaacgatgc
    37141 ttatcagcaa gcaaacagct ttttacgagg aaaagattat ctaaaatggc tgccaaagaa
    37201 agaaaaacat acgaaacaaa aaaacaaaga agatatccat gcgcattatg acttaggtaa
    37261 cgacttttat aaactttggc tagatccaac tttgacttat tcttgtgctt atttcaatac
    37321 acctgaagat acgttggaac aagcacaaat caataaagtc catcatatct tagacaaact
    37381 ctttatcaaa gaaggcgaaa cgttgcttga tatcggttgt ggctggggaa cattgatgtt
    37441 cactgctacc aaagagtata atgtcaaggc aactgggatc acactaagtg aagaacaata
    37501 cgatttgatt accaaaagaa taaaagaaga acatttagaa gataaatgcc gtgtattatt
    37561 aatggattac cgcgaactaa aaggcgaaac gtttgatcat atcacaagtg taggtatgtt
    37621 cgaacatgta ggttctgaaa atcttgaagg ttatttcaaa gtggtaaaag atttattgaa
    37681 accaaaagga acagctttga tccatggaat cagccgacaa caaggcggtg cgacaaatgc
    37741 ttggatcaat aaatatattt tccctggcgg ttatgtccct ggtgttactg aattagttga
    37801 taatattaca aaaaacgatt tgcaactgat tgatttggaa agcttacgtc gtgactacca
    37861 attgacatta gaacattgga caaagaattt tcatcaagtc caagatcaag tcacgaaaga
    37921 aatgggtgat tccttctatc gaatgtggga tctatattta caagcttgtg cagcttcatt
    37981 tgaatcaagt aatatcgatg tgattcaata tttgcttgtt caacctggaa acaatgacat
    38041 cccaatgcat cgtgcataat tgaggcaaaa gtactgttta aagactattt aaaaagaagc
    38101 taagttttat gatgctccag aagtgaactt ttttgtctaa cttctaggat tgtctctaaa
    38161 gcttagtttc tttttcttta tattttcata ataaaagttt ggttctttaa tttttaaaca
    38221 tgacagaaaa ttttttcttc gttatacttg taatagaata ctattttata acaaagggga
    38281 tcgaatcaat gaaaaaaact gttatttata ctagtttatt tgcagctttg tttttagctg
    38341 cttgtcaatc aaataccaat caaggcacat ctgggaatac gtcttccagt agcgaacagc
    38401 gctcttcaca ggtatcggac tcctcaacaa aagcaagcac atcttcgtct agccagacaa
    38461 aaagttctag tgaagcaagt actcagtcag atatcaaagt gtctgcagct cgtgcgatcg
    38521 aattgttcca agataaatac ccagatactg caattactag tttggaatta gatagtgatt
    38581 ggggatctta cttctacaag atagaaggtg tcgatgatca aaaagaatac gaagcaagaa
    38641 ttgatgctca aaatgaaaaa atggagacag agaatccaga aactctagat cgagatgaac
    38701 aaaatggtca aaagaaaaaa gaagatggtc tggatgttga taaattgatc acgattgagg
    38761 aagcagcaaa aatcgccgtc gacaaagtag gaagcggtac ggcaacagat tgggatttgg
    38821 ataaagattt aggtactact tattgggaag tcaaagtaaa agacggcaat caaagtacat
    38881 cagtcaagat caatagccaa acaggcgaag tactgtcaac tgaacaagat gattaagaat
    38941 ttcttcggaa aacaagagtt gatttttctt ttagtcatga tacactagac aaaataactt
    39001 gtttgaagga gcgatttact tgagtttaga aacgaccttt gccaatcata tctttgccaa
    39061 tcctttgatg aatgcatctg gtgtccactg catgactacc caggaattag atgaactggc
    39121 tcattcagaa gcaggagcat ttatcactaa gagctgtacg atcaatgaac gaaaaggaaa
    39181 tcccgaacca cgctattttg atgttccttt agggagtatc aattctatgg gacttccaaa
    39241 cttaggtttt tcttattatc tagagtatgc tcttgcgtat gaaaaagtgc aggaaaacca
    39301 aaatcaaccg ttattttttt ccattgccgg gatgagtgtc caggaaaact tggaaatgct
    39361 tgagaaaata gaaaaaagtg ggtttaatgg gatcactgaa ttaaatttat cttgtcccaa
    39421 tgtgcctgga aaaccacaac tagcttacga ttttgaggcc acttatgaaa cactgaaaga
    39481 agtattcagc atcttttcta agccgctagg aatcaaattg cctccttatt ttgattttgc
    39541 tcattttgat cagatggctg atatcttgaa tcagtttcca ttgacctatg tcaatgctat
    39601 caacagtgtg ggcaatggtt tgtatatcga tacagaacaa gaagcagtag tcatcaaacc
    39661 aaaagaaggg ttcggcggca ttggcggaga gtatatcaag ccgactgctt tggcgaatgt
    39721 tcgcgctttt tatacacgtt tgaaaccaga aatccaaatc attggtacag gtgggattcg
    39781 gacaggacaa gatgcatttg aacatctgct atgtggcgca agcatgttac agattggtac
    39841 cgagcttcat aaagaagggc cggaaatttt ttctagaatc ataaaagaat taacgcagat
    39901 tatgtcagaa aaaggatata cgtcaattga cgaatttaaa ggcaaattac gcaccatttc
    39961 atagcaaaga gacgaaggtc atgattgatc ttcgtctttt tttataaaaa acactcgtct
    40021 gttcattcct gtgcaagcgt actcattgta tgttagacga acttgtggga aaataacaga
    40081 ggcaaaaagg aggataatat gaatgatgca acagaattag caaaagagct taaagaaaaa
    40141 gaatttagtt ttcaagaact agttacggat atacagaaaa aaataaagaa gcaaaatcat
    40201 gaattgaatg cgtttgtcgc gcttgaacca ttcgagagta attcccacga gctattgatt
    40261 ccccagtcag aaaaagggcc attggctggt atcccatttc ctttaaaaat gctcggtcaa
    40321 gaaaaaaagg gatggctagc tacttcagga tctcgtttat ttgaaacaca ccgagcttct
    40381 catacatcta attatgtgcg gcaagcagaa gcgataggtc tagttccttt tggacaaacc
    40441 aatgcgccag agtttggttt caaaaatatt acagacccag tcatctatgg cccagcaaga
    40501 aacccttgga accttgaatg tacaccgggc ggatcaagtg gaggagctgc tgctgcagtt
    40561 tcatcaggaa tcgttccttt agctggtgct agtgatggcg gtggttctat tagaataccc
    40621 gcttcgtttt gcggattgat tgggctgaaa ccttcgcgag ggacgatgcc agttggtcca
    40681 tatgcttgga gaggatggca aggagctgct attgatttcg gcttgacagt ttctatgaga
    40741 gacacggagg ctttatttta tgggatgaga agtatccatt ccggtgcgcc atatcaagta
    40801 tcgccagttg agtggcaaac acatccaaga aaaaaacggt tgaagatagc tttttgtgtt
    40861 gattcaccga tccactcaca gattagtgaa gaggcagtac ttgctgtaaa aaaagctgta
    40921 gatttccttg gaaaagaagg gcatgatttg acagagattc cttacccact agatggtcgt
    40981 aaactgatca cgtcttatta tcagatgaat ggcgcagaaa cagcagcaat gatccgctcg
    41041 attgaaacag gcatccagcg agctgtcaga aaagaagaac ttgaacccat ttcttggact
    41101 ttgttccaat atggtcagaa gctctctgcc gctacttaca tcgattctct gcatgtatgg
    41161 gatgaagcag caattgtcat ggaaaaccta catcaagaat acgatctatt cctttcacca
    41221 acgactgcat ttaccgcccc taaaatcagc gaagatttgc aaagtgatgc gataagagca
    41281 cagatggaac acatcagtga ttgttctgaa gtagaaggtt tagacgttgt ttcttccatg
    41341 tttgaggaga gcttgaagat cacaccttat acacagttgg ctaatctgac tggacaacca
    41401 gcaatcagct tgcctactca tttgacaaaa gaaaacatgc ctctaggaat ccaatttatg
    41461 gctgccagag gaagagaaga tttgctattt caaatcggct atctatttga agcaaatcat
    41521 cagttccatt taccaaatcc atggaaatag cagaaacaag gagaagaaga atgaagaaaa
    41581 aaagattgtt tgctctaata actagtgcct tttttatttt aagcgcttgc ggtacagctg
    41641 ccgctaatca ggaagataac agctggagta atgtagaaaa aaagaaagaa ttagtagtag
    41701 cgacttctgg cacactgttt cccgcatctt attataatga tcaaaatgaa ttagttggtt
    41761 atgatgtaga tttggcaaaa gaggtggcta aacggttaaa tgtcaaaatc tcctttaaag
    41821 aatacaacgt ggatggacaa atcacttctg tttcaaaaag agaatcagat tttgctgcga
    41881 acgattttgg tatcacaaaa gaaagagaaa aaaaattctc cctttcagag actttcaaat
    41941 attcatttga cagtatgatc gtacgaaaaa aagatgattc aggaatccac tcacttgaag
    42001 atcttaaagg gaaaaaagca gctggcgaac ccaatactag ctatatgcgt ttagcggaaa
    42061 aacacggagc taaattagtc acatatgaca atgccacgaa tgatcagtat ttaagcgatg
    42121 tagcaaatgg tcgaacagat gttattctaa atgattatta tctgcagaaa atggccgtat
    42181 ctgctttacc agatatccct gtaaaaatac tggagaacgt ttatttcaat gaaaacgaaa
    42241 gtggtttttt attgaaagaa ggaaatgatg ctctgaaaaa gaaaatcgat ggcgtattaa
    42301 aagagatgaa agaagatggg acgacaaaga aaatatctga aaaatatttt caaactgatg
    42361 tttcagtcaa accgaaagaa acgatggctg agtcggtgag tgagtgatgt actttcctct
    42421 aattgatttt tccctcatgt ggacttcact tcctttcgta ctgcaaggct tggtatacac
    42481 attagggatt ggcttcgtgt cgtttctttt aggaaatctg gtagggttgc ttttgacagt
    42541 tttaggtctt ttggattggc tgcctctgaa tgtatttatt cgtttttatt tatcgttctt
    42601 tagaggcatt cccgcactag tcctgctgtt tctgttatat tttgggttac cttatcaatt
    42661 aagtgcctta acggcttcag tcatctgctt tacgattacc agcagtgcat ttattggtga
    42721 aatctatcga ggatcactag caggcgtttc ttcaggtcag tgggacgctg gtttagcttc
    42781 tggtttttct ttctttgggg tattgaaata tatcgttttg ccgcaagctt ttcgaatctc
    42841 agtaccggca ctcagtaacg tagctatgga tctagtgaaa ggtacttcgt tagcagcaac
    42901 aatcacagtg ccagaaatat ttcaaaaagc aaagatcata ggcggtcgga cattcgatta
    42961 tttttctctc tatattttag ttgcactgat ttattgggtc atttgtatcg ggatcgaaaa
    43021 agtgcaacag tatttagaaa aacgttctat gcatcagtca atctgaaaaa tagaaatggt
    43081 gctagcgtgg ctggcaccat ttctattttt tgtttcttgt atattctgcc tagtatttta
    43141 aaaccatttc atgtaaaatg taaatcgaaa atatgttcgg aggtagaaaa catgaagacc
    43201 ccgctaacca tcgaaataga atcctcgtta taccaatatt gcatcgagca aggcggcttg
    43261 gttgtagaag aagtcacgat gcctgatgac caaggaattg tagatacgct tagctgcttg
    43321 acaaaatttg atggaagccg agaatggcga tgctatgagc tgaaagtgac aaaagcagat
    43381 tttcgctcta cagccaaact gtcatttgtt ggacattata attattttgt tttaccaagt
    43441 aaactatatg aagaagtaaa aactgaaatc cctacaggaa taggtgtttt agtgtatcgt
    43501 ccgtacacgg ttgaagagga gatgcctgcc tctggtacat ttctgattgc gaaaaaagca
    43561 gcaaagaaag agcttgctgt accggaagaa gctttgacaa accgcttcat ggcttcttta
    43621 ttcagggaag taagaaaagc aaagcaaatg gagcgaggcg taaaatattt tccaacagat
    43681 caattgtaca aagaattgaa aagacgttca gaaaatcaag atccattttc taacgaacga
    43741 tattatgaac ggtttttaga ggaaatagct agtcaggcaa tcaatgagtt gcaagaagaa
    43801 gttatcgcgc ttcagcatga ttatgaatat ttgaggcaac aacagcagat ccgtcggttg
    43861 cctacagagc cattggagtg aaaaatatgt actatcctta tttaagagga aaacaatttg
    43921 acctattagc attaaaagaa gcactgtcgc gaggattgct ctccaataaa atccaaccag
    43981 tgatcgaacc agtacgtgat tccgctacgc ttaaaaatgt gatcgaatta tttcaaaaaa
    44041 agcaccatcc aatcgctgtg atccaaaatc cccaagtagg acaattcaaa ttattcgatc
    44101 agcatgtgca cagttggaca gtcaaagaaa atagctctgt cgtccctgca cagatcttaa
    44161 cgccagaaaa tctcagtgaa gtactggatt cacctccagc gtttcttgtt ttcgatggac
    44221 agcactaccc aaaagataca gaggtgtgga aacagttggc aggagttgat tcgaaatttt
    44281 tgataccaga tactagtcgc tttcgcatct ggctgcccga aaacaaaatt gtgatccgtg
    44341 actcttttca aacgagaaag catgttgaaa gttatgcaga taaaacagat gactttttta
    44401 gtgatgacta tctttttttc cacgcagacg gatatattgg attttctgat ttcacgatcg
    44461 aaggtagccg ttatttcgat aaagggttcc ctagccgagc gcttgctatc catctaacgt
    44521 atattgatgc ttacggcaat atacgcgtga aacattttgt ctctgacagt aacgactctg
    44581 ctaaagacca agcattgaag tttcttgaag caggagataa aatgaaaaaa tggatcatgc
    44641 gtcaccatca tcaactcttg attacttcag gaatgattga attattggct ctttaccagc
    44701 aacaaaaatt ccctggactg ggagtgttga aaaagtggtc acttatgcat catttggaac
    44761 ttattagtca cctgctagat catccaaaag attggcttaa atattattca aaagttcagg
    44821 aactatatga agtgatccaa tgattagata atgtaaatga aaaaggaata gaaaagagga
    44881 aactggatgt atattcggca aacaaggaaa gaagaagtag aagaagtggt agaaatcatt
    44941 aatgccgcag cggatttcat tgcttcgcaa ggctctcctc aatggcaaaa cgggcagaaa
    45001 ccaacaaaag aaacgatttt gaacgatatt gaaagaaaag aaagctatgt attaatagtg
    45061 gaagatcaaa ttgctggaac agctgcatta gtctcaagcg tggatcccgt ttacacaaaa
    45121 atagatggta gttggaaaga atcaactggt ccttatttat cgattcatcg tgtcgcttta
    45181 gcagaaagta ctcgaggaaa aaaactagca aaaccgttct tagaacattt gttgacgatt
    45241 gcagccagca aaaatatcca agatatccgt atcgatactt atccagctaa tgaaccgatg
    45301 gaaaagacca tctattctgc tggattcact tatcaaggga tgattgaatt tccttttgaa
    45361 cacggcgaaa gaaaagcata tcaaataatc atcaattgac tataaaatca tctgagcaaa
    45421 gataaagaca ttaacactac tgaaaaaaac aatcatatga tagccaagag gttgtaacag
    45481 aagtggccag cttcaaaaaa caagaaagaa tttccgaaaa tgcttctcaa atttttgtgg
    45541 aattttagcg tattttacga aatagctgct ttctgaaact cccttcatcg agaggtcgtg
    45601 acatagcttt tgtcacgacc tctcggctta tcctacgagt attattcgat acatttatat
    45661 caataatcct ttttttattt tatctgcagc ttcttggaac gctccatcgt ttgacacgac
    45721 taaaagtgag gggatagctt tcaaacgttt caactgtgct aaatcttcta cataaaccgc
    45781 cagtctggct gaaatctgtt cagatgaatc tccacgtttt ttcaggcgcg attgcaatgt
    45841 ttctttagag atcgtaataa agataggacg gactaccgtc ccgaaatact catataaatg
    45901 ccaaaaacca tttgctgtca aaacggcgta acaatttcct actgtcagtt tctttttaat
    45961 ttctgcttta ctggaaccat atttatgttg agcatattgg tcccactctg caaactcatt
    46021 tcccttgatc atctgattga atgtattgtc agaaacaaaa taataatcct cttgatcttt
    46081 ttccccaggt cttggtgatc gattcgtgta agtaatgatc ttttgttccg gctgaaaaat
    46141 agcttctgcg agtgaagttt ttccagaacc agaaggaccg ataaatacat agcaataatt
    46201 cattgtcatt tctcctttga tgattgaaca agtgaaaaag ctttggaaaa tatttccaaa
    46261 gcttttaaaa gtaactttgc ttttttgtat attataccag caagccagtc acaatagcac
    46321 ttaaaaagct gactaaagtc gctgttagca aaattttcaa tccgtgtttt gcgacaagat
    46381 ttcctttttc ttcatttaat cccttcatcg caccagaaat gatcccgata gaagagaagt
    46441 tggcaaatga aacgaggaag acagatataa ttgctgttgt acgctcagta aaatggaaat
    46501 taccatttgc taattctgtc atagcaacaa attcatttgt aaccagttta gtcgccatga
    46561 tactaccggc atccactgct tctttccatg ggattccgct gatgaaggca agaggggcaa
    46621 agatgtaacc taggatctct tggaaagtga tgccgaatat cccgtggaag atagcattga
    46681 tcatggcaat caaagcaaca aaaccgataa gcatagcagc aaccgtgatc gccacatgga
    46741 aaccatcaag aatgtattct cccaacattt ggaaaaatgt ctgttctttg ttttcttcaa
    46801 tgatcagctc atcttctttt tcttctaata catagggatt tacaattgaa gccagaatat
    46861 aaccgccaaa taagttcaaa acaagggcag tgatcacata tttagaatcg atcagtgcca
    46921 tataagatcc gacgatcgac atagaaactg tagacatagc cgatacactc atggtgaaca
    46981 aacgtttctc agataggaag ggtaattcct tttttatgga aataaaaact tccgattgtc
    47041 ctaaaatagc agaagctact ccattataag attccaattt gcccatcccg ttgattttac
    47101 ttaatcctag accaacagct ttcataaata atggaagaat cttgatatag cgtagaatac
    47161 cgataatagc agaaataaat acaattggca tcaatacgga aagaaagaaa ggtgcacttc
    47221 ctttatttgc tacaccggca aaaacaaagt caacaccttg cccagcaaaa gcaagaagat
    47281 gatcaaacat ttttgccaat acagaaacaa ccgctgtacc aaaagttgta cgtaataata
    47341 cgaaaccaag aacaagctgt aaaacgaaca tcacaattag tggtttcaat ttgattgctt
    47401 ttttgttact gctgatcaaa aaagcaagcc ctaatgtgac aaataaacca ataataccta
    47461 ataaataact caaataatca ctcctattcc aaattccgtt ttattttacc tgttttgcaa
    47521 ataaaatact aatctttagt gaaattttca ttactttttt agagtgaaac gcattcattt
    47581 tccattagaa aataaagcga acaaaataaa aggtttaaaa agtgtaacgt cttagtaaag
    47641 tctataaaaa aacgaaacta tcgattaagc taatagtttc gttttcaaca tttataagta
    47701 aactttttct attggtaata tagattttcg gaagggtaga caggtgcgca ggtaatatcg
    47761 attcctagtt ttttcaatgt ttgttcatca tttttattga ccatcacagt agagtgggct
    47821 tgtgctccct caagttcagc tagtttatca taagcaactt gagcgattgg attcgttact
    47881 gcactgattg ataaagcgat caagacttca ttgatgctta gtgcatccaa agaggaatga
    47941 agtgtctttg atttcaatcc ttgtattgtt tcaagaacta atggtgacaa taaaggaatg
    48001 tcatctgcga tatgggctaa atgttttact gcatttaaga taactgccgc tgacgcatcc
    48061 atcaatgaac ttgtacgccc ggtaatgatg acttcatctg gtaattcgat tgcaatcaca
    48121 gcagaaggat cttttgtagg actttgcgct tgaatcttat ctgcatagtc atgggcaggg
    48181 actaccacgt ttcggtcttc aggacgtaaa ccgacttctt ccataatcag tttgattcga
    48241 ttaactgctt cttcgtcgat caatcctttt ttgaagtcgc attctgtttg gaaataacga
    48301 cggatgattt cttgtttaga ggcttcttgg acaacggtat catccacgat cccaaatcct
    48361 acacgattca cacccatgtc agtaggagat tgataaacag attctttacc agtaattttt
    48421 tcaataatac gtttgattac aggaaatgtc tcaacatccc gattatagtt cacagctact
    48481 ttattgtagg catcaaagtg gaaggaatcg atcatattga catcttttag atcgactgtg
    48541 gctgcttcat aagcaatgtt cagcggatgt ttcaacggaa cattccatac tgggaaagtt
    48601 tcgaacttgg agtaaccagc tgcatgtcct ttttggcttt catgatacag ttggttcaag
    48661 gaagtagcga gctttccact tcctgggcca ggagctgtca ctacaacgat tggtttagtt
    48721 gtttcaatat actcattttt tccaaaacca tcttctgaga cgattttttc tacgttgact
    48781 ggatagtctt cgatttctga gtgtttatag accttgatgt ttcgtctttc tagtttattg
    48841 ataaagactt tcgtggcagg ctgtccacta tagcgagtga taacgacact gttgattaat
    48901 aagccataac cacgtaattc atcgattaac cgcaagatat ccatatcata agtaatccca
    48961 taatcaccac ggatcttatt acgttcaata tctccagcat agacgcaaat cagtatttca
    49021 gcttgatctt tcaatttttg cagcaatttg attttcgcat cttcttcaaa tcctggaagt
    49081 acacgtttcg catgcttgtc gtcaactagc ttcccaccaa attctaaata taatttgtca
    49141 tagtggttca ctcgttccaa aatatacgct gactgctctt caatatattt ttctgaatca
    49201 aaaccaattt ttttcactag gttccctcct taagtactaa agcatttttt catttttttg
    49261 aggattcgtc aactggaaga gcgaaattcc tcaaaaaaac aaagaaaatg gtttaaacca
    49321 acttgtctag atagttattc attggtatga tagtttttga taaggaaggt gctacatgaa
    49381 aaaaagacag aaaaagaaaa acgcatataa acactatatc cgttcgattt ttaccggtta
    49441 tgaaagaatg cttgaagatc ccgaattgga acaattgacg tttacttatc taaacgaaga
    49501 aacgcagcta actcgtgatg agcatcagcg cattcatttc acaacaagag atctgcctag
    49561 taaataaaaa ataagataaa ggtcatcgga ttcgattctg tcctatcaga tggaaagact
    49621 agttgatcga aaaaaaggct gtaccaaatt ttggtgcagc ctgttttatg gagcattgcc
    49681 cgctcttttt tataacaatt ttttacagaa aatggtacta ttgaagtatt aataagtgaa
    49741 aaacagatga gtaagaggag gcaagtagta tgaaagagcc agatacaaat gcaaattatg
    49801 agccagacga actagaggag acgaatgcgg atcaggatac aggacaagaa tcgtcttctt
    49861 tttacgatat caaagaaaaa gcaagcatga atctttccca attattagaa gacttacagg
    49921 cttttgaaaa agctgtccgc gaagaagata tccctgagat ctaccgttta tacaatggcc
    49981 gattgaatga agaattaaaa agaagctcaa atgaaaatca cgagatcgat caattgctga
    50041 tgcgtaagat ccatgatcgt tttttacagg aatttccttt tatggaacaa gtagaaaaca
    50101 tctcgccaac gatgagctat tacaaaatcg gcacttatta ccatgatcgg cccacggtcg
    50161 gtattgatgc aagtctgcca gaaatttttg ttttgcctaa gatcgatgaa gagtgggaga
    50221 aatattccca accggagtca acggactatg acaaaaaaat caatgagcta gacgcaaagg
    50281 tgatcactgc tcagacagaa attgaacgtt tagatgaaca aataaaagaa atcaatcgac
    50341 aaatgactga tttgaacgac aataaaggat tcttgaatcg taaaaaaatc gaggaagaaa
    50401 ttcaagaatt agagaagaag aagcaagtac tatctaatga aaaattaggc tggcttcctt
    50461 atattgagac gcctgaaaca atccagcagc aaaaagaagc attgaagcaa gaagcaagag
    50521 cagaccagct tcgtgcagcc atcgtagaaa aagaacaaag gcaaatcaga cgctatttcg
    50581 gtagcaaaga gggattcggt caagcaatcc atgaattctt gatgaattat cttggcaatg
    50641 agaatcctaa taatcaatct aatgaaggag gttccgagta tgaatgaaca cgatgaagca
    50701 gaatggacaa aaaatgaaga acaaacagaa gatcatgaag aaacgaacac ttcattggaa
    50761 gaacaaacaa gtaatcctct ggatgacaca gaagaaatcc ctatgctaga ggaaacaaaa
    50821 atacacgaaa aagaaaaagt aactccgacg aatacgaata ctgcagaaaa cacccatgct
    50881 tacgaaataa ctgaaaatcc cgggtattta gaaggaaaag agcaagatca tcaagatatc
    50941 gtcagtttga ttgaaacagc ggaagcagaa ttagcggatt tacgtatgag aaaagatttg
    51001 atcgaatcag attttccaca attattagaa tcatatgatc gttttatatt gaatgaagaa
    51061 ccaatctcca cgatcggaaa gacaagcctg atcgaatact tcacgtatga attactgaaa
    51121 ccgttgaatg atattggtct ggaacggtca aattcagctt atgacacatg gaaaacaaga
    51181 catttttcga tcaattatac gttgaatgga caaaacgaat tagtcttttc ctttgtatta
    51241 ccgaccatcg atcaacgata ccaagtggaa tcgatgagtc tcctaaacgt atcaccagag
    51301 acaatggaaa tcaatgtcca agatgaccgg gtgctttctt tgatccgaca ctggtcagtg
    51361 gatcgagtat tctctgcagg gcagatcacg atattcaatc acaaattgaa tcaattatta
    51421 gcccatgcaa gaacacttgg ttttaccgtt aatcagacat tactagacaa tacgaagcca
    51481 cttcgtttga atcttcagag cgaatttgaa ttgacgaatg aagtattgga tgatattttt
    51541 atcgtggcaa tgaaaaatcc atcctacgat atggaaaaga tcaacgaaac tacttatcaa
    51601 gtacgattag ataaaggaca gtctttaacg ataacaacaa acgaaaaaga tcaaacagtc
    51661 ttgtcgatct catctggaga ctacccacga tctgtcattg atttctttat caattacgaa
    51721 ttcttggtcc cattaatggt gagaaagtcc tgattggata agttggatta ccagatgtca
    51781 ttcagataat tctgttccaa aggacttgcg gcagttatcc agaataattg tcacaagtct
    51841 tttctgtatg tactaaatgg acgtgaataa cttgctgatg aaggagggat ttcattagga
    51901 atgtccacaa ataaaatagc acagcttagc ttattgtctg ctgcttgtgt agcaggtagg
    51961 atcgcttttc aatttattcc gaatttccag ccaatgactg ctattttttt atttatagta
    52021 ctttacttaa gcttaaaaga tgcgttaatt gtcatgagtc tttctattgc gatcagcagt
    52081 ttttattttg gtgttggacc atgggtcatc ggccaatgga tcagctatac tgttgttctg
    52141 agtttgtttt caatcgtctg cttgagaaaa acagtgcaaa ggaatctatg gttaaaggga
    52201 gtctgttttt ttctggcggg catggtttac ggaataggta tgaccgtttt tgatacgctg
    52261 ttatatcgtt tgcctcaacc ttggatctat taccttcaag gggtgtcgtt tgatttgatg
    52321 cacagtatag gcaatgttgt gttctttctt gtttttctgc cagtcgtcaa acgtttttac
    52381 aatcattcag gaggaaaaaa tgaaaaaaat aatttatagt ttgcttgcaa tgagtgtcag
    52441 tatactcatt ttgacaagtt gcagtacaac ctatacacaa tcggcttcca accaaaagga
    52501 ggaagtaaaa acatcgcaaa aagcaacaat cacgttacaa gaaaatggtc aaaacttttc
    52561 ggaaaaagaa gtgttcttca aaaaaggcga tgatttactg agtattttga aacgaaattt
    52621 tgaagtcaaa gaagataacg gcttcatcac ttcgcttgaa gggcactcac aggatgagaa
    52681 aaatcaactc tattggatgt ttactgtgga cgggaaaagt gctactactg gtgccaaaga
    52741 gatcaaattg aatgatggac aatcggtcat tttcaatttg tctaaactat aagtttcata
    52801 aaaaactgtt aaagggcgaa tattgccata attcagtgag ttcagttgat gaaagaagga
    52861 aaataacatg gaaaatttta aaaaaagcgg aatgttttcc gtaattgctg ctctaggggc
    52921 taatattcta gttgctatca gcaaatttgt cggctatgca atcagtggca gtgccgccat
    52981 gctaaacgaa agcatccaca gtatcgttga ttgcagcaat cagattttgc tgttattcgg
    53041 tgataaacaa gccaataaag gacagagcga gttgcatcaa tttggtgaag gacgagcaaa
    53101 atactttttc agtacaatcg tcgcaatgat gcttttcttc ggcggtgggg cacttggggt
    53161 gatggaagct tttgaaaagt tgacgcatcc atcccatgaa gtgggtaatc cgatcattgt
    53221 tatagcaata ctgttatttg gtttggtaat cgaaggaagt tcgttacgcg tggcaatgaa
    53281 agaaatcaaa gaattgaata ctgaaaaact ttccacgatg cgctttttgc gcgaaagtcg
    53341 acacagtgaa atactgatta tttttacgga agacttgtgt gctgtaatcg gtttggtctt
    53401 ggctttagca ggaacaatat tgacttatgc aaccgggatt gctgcatttg atgcgatcag
    53461 cggattgctg atcggtttct tgctgatggg agccgccatt ttcctagcaa aagaatttta
    53521 tagtctgatc atcggtgaaa gcgtgacagc aagcgattta tcaaaaatca agttagcttt
    53581 tgaacgtcct gagatcagtc ggctgatcga tataaaaaca gttcatttaa gtccaacgga
    53641 gattttagtt gcagcaaaag cagatatcgt tggtaacaaa gagaatgaag cttactcggt
    53701 catcaatgat attgaaaaaa atatgcgtca gtctcttcct gacaagaaga tgtatatata
    53761 cattgaaaca gataaatatg acccaaatta ttctcgtaaa taaaacgttt attcatgaat
    53821 tgaagcaaaa atgagaaaaa tataatcttg tttttaaata aaacggctac aatttagcga
    53881 atttatgtta caatgagtcc gctgtttatt tgttgtaatt aaaggagata ttttaatgaa
    53941 caaaaagaaa agtgcgccat ggcttcaagc tttgcatgca gtgatgccac tttgtataag
    54001 ctatatacca gtaggattag cttgcggagt attgttgcaa aaagttggat ttgatcctct
    54061 cttatcagga ctgttatcga ttctggtctt ttccggtggc gcgcagtttt tagttgcatc
    54121 tatgcttacg acgcaagcat catttgcaac gacattattg atggttttct ttttagaatt
    54181 acgctataca ttattaggag caagtcttgc cggttttatg aaaaaagaaa agcggacgtt
    54241 tttggctgtc ttttctcaat cactgaatga tgaaaactat gcggtgaact acttaaagtt
    54301 ttcaactgac ccttcctgga ataaaagaaa ggctttatat gtcaattggt tctcaatgac
    54361 tgcatgggcc ggtagcaata tgatcggtaa ttttttcggc tcggtgattc gagtagatgc
    54421 tgaccttgtc cattttgctt tgacggccat gttcattttc atgtttatca tgcagatgaa
    54481 aagcgcacta ctgatcttta caggtttatt ctcaggcgta ttaggggtcg tattcatggt
    54541 attattccaa aatactttcg gattgattgc agcaacagtc attgcttcat ttgccggatt
    54601 tatgctggag aaggcattca aaaaagaaaa agctaagaaa ctaagagacc gcggtaaaga
    54661 tgtcaatgag ccgctcttca acttcccgga acaggaggcc aatcaccatg actaaactat
    54721 atttgctact tttagttgct tgtctgttca tcgtggcata tatcccgcgc ttgttcccga
    54781 tgctttattt tactcatcga aaagtgccat cctggttcag tgactggatg aaatatgtac
    54841 cggtggcatt atttgcggcg ttagctttta aagatgtttt cattactcat gaacatctgg
    54901 atattgcttg gaatatcaaa atagctgcaa tgattctagt agcaggcgtg gcttacaaaa
    54961 cacgttcgat ggcgttgtca gttttgacag gacttgcgtc tgtctttttg ctgtctatgc
    55021 tttaaactgt tttattttat taaaaacaga gattgcgaca taagttgttc aaactgatct
    55081 atccgaactg catttgcaac agtcaattct ggatgcaagt ttattcgtag ggataaaaaa
    55141 gattagtgtt ggcttatgat gcaagctctt ttttatttta ggaattagag agggatttgt
    55201 ccttctatga ttttttgcta taatagggaa gatgtttatt ttatgaaaga atagttgagg
    55261 gaatttaaat gaataaaaaa ttgatgcaag gaacattttg gctaaccttt gctaatcttc
    55321 tatgtaaagt tttaggggta gtctatttga ttccctggct aagtatgatg gggaacaatc
    55381 aagatggtat gcttgctacg acattgtata atgttggcta tcttccttat ggattgttcc
    55441 tgatgcttgg tacagtcgga tttccaaatg cgattgcaaa aaaagtagct gtagccacaa
    55501 aagaaggaga taacgctgct tgtcggctga tttttagaag tacgatcaat atcatgtttg
    55561 tcatcggtat cgcctctgct gcactgatgt atttatttgc tcctttacta gccggaatca
    55621 gtccgattgc taacgttaat aatgggatac tagctatcag aagtttatgc ccatctttga
    55681 ttgctatccc aattcttagt gcaatgagag gatatttcca aggaaaaaat gatctgcggc
    55741 cttatggaac ttctttgatt atcgaacagg ctgtgcgagt gatcgttatc ttggctggta
    55801 cttattactt gcgcgtattg actgacggaa ccatattaga agctgtattg atcagtacag
    55861 tcgcttcttt cttcggtggg ttagctgcga ttatccatat gtatatcgtc ggacgcagaa
    55921 aagacttctt cgaacttaaa gattttttga tttccagccg ttatttcgaa agagaaaacc
    55981 gatctgcctc tgtctctatc attcgggaaa cactaccgtt tatttttgtc ggctcagtca
    56041 ttactatcgt tcaaatgatc gaccaagtaa cgatgaaacc actgcttcac tttttccgtc
    56101 cagagatcgc agatcagcaa ctggaattct tattcagccg tgcttctgtc aatccaaata
    56161 aattgacatt gatcctgatc tccatggtag gaacagtagc tataagcagc ttgccaatat
    56221 taagcacgat gaagaaaaaa gatcggatac aaatcgaaaa aacagtagga gatagttttt
    56281 cgattgcgtt attgattctt cttccttcat tgactggaat gtctttatta gctggaccac
    56341 tatacacatt gttttttggc tatgatcctg aaagtgtcgg atatttccaa atggctttgc
    56401 tagcttcttt gtttttctct ttattcacga tcctatctac gatgcttcaa tctttgaatc
    56461 accatcgttt tgcgatccga ttaactgtag aagcaattat cctgaaagtc atcttccaag
    56521 taatcggatt aggattagtt ggcggatacg ggatgagctt atcaaatggc ttagcattcg
    56581 gtattgtctt tgttcgcggc tatcacttta tgtgcaaaga ataccgaata gcacctttag
    56641 caaagatcag caattttttc ttaaaaactt tccgctccac cctgattatg cttgcagtat
    56701 gttttgccgt tttctttttg ctgaatcagc gattgtcaat ggaatcaaaa agctatgcaa
    56761 tgatttattg tgtggctgta ggaagtattg gcggcttagt atttttattt tctcaattcg
    56821 gaaaaaatgg gtttcgaatg ttgaaaaatg ttaaacatca tagcagaagc aaagaatagg
    56881 ttaactaatc gttttttgct gtcacttgaa aaacggcaac tcgtatgatt acgcttgtcg
    56941 tttttctatt ttccaatttt agtttcatat ctaatcttac ttatgatata gttataaggt
    57001 ctggaaaaat tctttgaaac gaggaaatta aacacatgaa acttgcagta gttacagata
    57061 gttcggcata tctgccggaa cgtatcaaga accatcccga cttattcgtt attccaatcc
    57121 ctttgattat ggatgggaaa atttataacg aaggaatcga tatcgaagcg gatgaatatt
    57181 atggactttt aaaaaacagt aaagattttc ctactacttc acaacctgtc gtaggggaag
    57241 ttttgggttt atatgaggaa ttgaaagaga aagggtatga cacgatttta agtatccatc
    57301 tttcttcagg gatttctggc tttgtcaaca cattatttgc gatgaaagat gacatcagcg
    57361 gagtaacggt cattccttat gactctaaga tcactagtat gccgatggga cacatggtag
    57421 aagctgcctt agacttaaat gatgaaggtc agtcccttga tgaaatactt gcacatatcg
    57481 atcgaatccg agataatacc tatgcttatc tgattgtaga tgatttgaac aatttagtac
    57541 gaggaggacg tttgacaaat ggtgcagcgc tgattggcag cttattaaaa atcaaaccga
    57601 tcctgacatt ttctgaaggg aaaattgttt tattcgaaaa aatccgttca agtaaaaaag
    57661 catttgctcg agctgaagaa attattggta gaagaaataa cgaaatacaa ccgccagtca
    57721 aactttacgt gatacatgca aataatttag aagtagcgat tgcggagaaa gagaaattgc
    57781 agaaaaaata tccaaatgca gaaatcgaaa ttggtcattt cggaccagtc attggtacac
    57841 atttaggtga aaaagccatc ggcattgcca tttcagcgca ataattcctt ttattcaaaa
    57901 aacataccaa aaat
//
LOCUS       DACDXE010000009        57233 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.009, whole genome shotgun sequence.
ACCESSION   DACDXE010000009 DACDXE010000000
VERSION     DACDXE010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.009"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            281..973
                     /locus_tag="IWM23_000528"
     CDS             281..973
                     /locus_tag="IWM23_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9883198.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1181..1471
                     /locus_tag="IWM23_000529"
     CDS             1181..1471
                     /locus_tag="IWM23_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883199.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            2185..3555
                     /locus_tag="IWM23_000530"
     CDS             2185..3555
                     /locus_tag="IWM23_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883200.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(3795..3878)
                     /locus_tag="IWM23_000531"
     tRNA            complement(3795..3878)
                     /locus_tag="IWM23_000531"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3842..3844),aa:Leu,seq:gag)
     gene            4261..5478
                     /locus_tag="IWM23_000532"
     CDS             4261..5478
                     /locus_tag="IWM23_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9883201.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            5662..6498
                     /locus_tag="IWM23_000533"
     CDS             5662..6498
                     /locus_tag="IWM23_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9883202.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            6574..7722
                     /gene="nagA"
                     /locus_tag="IWM23_000534"
     CDS             6574..7722
                     /gene="nagA"
                     /locus_tag="IWM23_000534"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAP9883203.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            7942..9000
                     /locus_tag="IWM23_000535"
     CDS             7942..9000
                     /locus_tag="IWM23_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAP9883204.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            9019..10038
                     /locus_tag="IWM23_000536"
     CDS             9019..10038
                     /locus_tag="IWM23_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAP9883205.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(10140..10856)
                     /locus_tag="IWM23_000537"
     CDS             complement(10140..10856)
                     /locus_tag="IWM23_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9883206.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(10825..11610)
                     /locus_tag="IWM23_000538"
     CDS             complement(10825..11610)
                     /locus_tag="IWM23_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9883207.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(11601..12350)
                     /locus_tag="IWM23_000539"
     CDS             complement(11601..12350)
                     /locus_tag="IWM23_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAP9883208.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            12648..13172
                     /locus_tag="IWM23_000540"
     CDS             12648..13172
                     /locus_tag="IWM23_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883209.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            13188..14687
                     /locus_tag="IWM23_000541"
     CDS             13188..14687
                     /locus_tag="IWM23_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAP9883210.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(14775..15377)
                     /locus_tag="IWM23_000542"
     CDS             complement(14775..15377)
                     /locus_tag="IWM23_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9883211.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            15481..16437
                     /locus_tag="IWM23_000543"
     CDS             15481..16437
                     /locus_tag="IWM23_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAP9883212.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(16742..17698)
                     /locus_tag="IWM23_000544"
     CDS             complement(16742..17698)
                     /locus_tag="IWM23_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9883213.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            17872..18432
                     /locus_tag="IWM23_000545"
     CDS             17872..18432
                     /locus_tag="IWM23_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883214.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            18425..19249
                     /locus_tag="IWM23_000546"
     CDS             18425..19249
                     /locus_tag="IWM23_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAP9883215.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            19259..19876
                     /locus_tag="IWM23_000547"
     CDS             19259..19876
                     /locus_tag="IWM23_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAP9883216.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            19894..20577
                     /locus_tag="IWM23_000548"
     CDS             19894..20577
                     /locus_tag="IWM23_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAP9883217.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            21260..22495
                     /locus_tag="IWM23_000549"
                     /pseudo
     CDS             21260..22495
                     /locus_tag="IWM23_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            22503..23390
                     /locus_tag="IWM23_000550"
     CDS             22503..23390
                     /locus_tag="IWM23_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883218.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            23407..24243
                     /locus_tag="IWM23_000551"
     CDS             23407..24243
                     /locus_tag="IWM23_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9883219.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            24246..24869
                     /locus_tag="IWM23_000552"
     CDS             24246..24869
                     /locus_tag="IWM23_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883220.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            24866..26158
                     /locus_tag="IWM23_000553"
     CDS             24866..26158
                     /locus_tag="IWM23_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9883221.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      26517..26614
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            26730..27311
                     /locus_tag="IWM23_000554"
     CDS             26730..27311
                     /locus_tag="IWM23_000554"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAP9883222.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            27314..28621
                     /locus_tag="IWM23_000555"
     CDS             27314..28621
                     /locus_tag="IWM23_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAP9883223.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            28914..29402
                     /gene="purE"
                     /locus_tag="IWM23_000556"
     CDS             28914..29402
                     /gene="purE"
                     /locus_tag="IWM23_000556"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAP9883224.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            29386..30519
                     /gene="purK"
                     /locus_tag="IWM23_000557"
     CDS             29386..30519
                     /gene="purK"
                     /locus_tag="IWM23_000557"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAP9883225.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            30532..31827
                     /gene="purB"
                     /locus_tag="IWM23_000558"
     CDS             30532..31827
                     /gene="purB"
                     /locus_tag="IWM23_000558"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAP9883226.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(32007..32384)
                     /locus_tag="IWM23_000559"
     CDS             complement(32007..32384)
                     /locus_tag="IWM23_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883227.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            32640..33368
                     /locus_tag="IWM23_000560"
     CDS             32640..33368
                     /locus_tag="IWM23_000560"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAP9883228.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            33368..33622
                     /gene="purS"
                     /locus_tag="IWM23_000561"
     CDS             33368..33622
                     /gene="purS"
                     /locus_tag="IWM23_000561"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAP9883229.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            33624..34295
                     /gene="purQ"
                     /locus_tag="IWM23_000562"
     CDS             33624..34295
                     /gene="purQ"
                     /locus_tag="IWM23_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAP9883230.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            34296..36518
                     /gene="purL"
                     /locus_tag="IWM23_000563"
     CDS             34296..36518
                     /gene="purL"
                     /locus_tag="IWM23_000563"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAP9883231.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            36503..37942
                     /locus_tag="IWM23_000564"
     CDS             36503..37942
                     /locus_tag="IWM23_000564"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAP9883232.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            37974..39017
                     /locus_tag="IWM23_000565"
     CDS             37974..39017
                     /locus_tag="IWM23_000565"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAP9883233.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            39014..39592
                     /gene="purN"
                     /locus_tag="IWM23_000566"
     CDS             39014..39592
                     /gene="purN"
                     /locus_tag="IWM23_000566"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAP9883234.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            39589..41124
                     /gene="purH"
                     /locus_tag="IWM23_000567"
     CDS             39589..41124
                     /gene="purH"
                     /locus_tag="IWM23_000567"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAP9883235.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            41140..42414
                     /gene="purD"
                     /locus_tag="IWM23_000568"
     CDS             41140..42414
                     /gene="purD"
                     /locus_tag="IWM23_000568"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAP9883236.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            42536..43450
                     /locus_tag="IWM23_000569"
     CDS             42536..43450
                     /locus_tag="IWM23_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAP9883237.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(43583..45148)
                     /locus_tag="IWM23_000570"
     CDS             complement(43583..45148)
                     /locus_tag="IWM23_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9883238.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(45141..46592)
                     /locus_tag="IWM23_000571"
     CDS             complement(45141..46592)
                     /locus_tag="IWM23_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9883239.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(46606..47622)
                     /locus_tag="IWM23_000572"
     CDS             complement(46606..47622)
                     /locus_tag="IWM23_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9883240.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            47842..48930
                     /locus_tag="IWM23_000573"
     CDS             47842..48930
                     /locus_tag="IWM23_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9883241.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            48984..50510
                     /locus_tag="IWM23_000574"
     CDS             48984..50510
                     /locus_tag="IWM23_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAP9883242.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            50512..51717
                     /locus_tag="IWM23_000575"
     CDS             50512..51717
                     /locus_tag="IWM23_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883243.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            51974..52951
                     /gene="guaC"
                     /locus_tag="IWM23_000576"
     CDS             51974..52951
                     /gene="guaC"
                     /locus_tag="IWM23_000576"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAP9883244.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            53215..54639
                     /locus_tag="IWM23_000577"
     CDS             53215..54639
                     /locus_tag="IWM23_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAP9883245.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            55100..56209
                     /gene="pdhA"
                     /locus_tag="IWM23_000578"
     CDS             55100..56209
                     /gene="pdhA"
                     /locus_tag="IWM23_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAP9883246.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            56212..57189
                     /locus_tag="IWM23_000579"
     CDS             56212..57189
                     /locus_tag="IWM23_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAP9883247.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        18451 a         9703 c        12410 g        16669 t
ORIGIN      
        1 cgttttttgt aataaataca gtaatctatg aaatgaaaag ggttacgttt aataaaactt
       61 gcttttttgt ttttgttcac cataaatatt tatttaagaa agtattaacc tgcatttttc
      121 tctataaaaa agatttttta aatagataaa aaaggaggat taaatgagta tttgatagta
      181 tttatattaa aattacacat gtaatttaaa aacgcttgac attgtgtttg agatcactta
      241 cttttagcat gtaaaagtat tggattaagg agctatgcaa atgaaaaaga aatttattac
      301 tggtgggatt gtcgttgcat ttctatgtct aattggctac acaggcacgc ggtacgcaaa
      361 tgaaattatt ttgtggggtg gagagacaga tattgataca atcaatgaga atctgacaca
      421 gcttgatcat acattgacag ataaagagca agcgcttaca gatcttaccg ctcgactagc
      481 acaaaaagaa cagacattgc aagattcaaa ggcagatgtc caagaatacc aaacaaaagt
      541 aaatgaatta gaaaaccaaa aaaatcaatc ggcagcagaa aaacaaaact tagaaagtca
      601 attagtcagc aaggatgcac aactacaaag caaacaaaat gaaatcaatg tcaaactaga
      661 tgagatcaac caaaaaaatg atgaaatcaa tcatctgaat gcagattgga cagcaagact
      721 acaagaagca caagcaaaac tttcagaatt acaaaatact gtaaatggtt tgaatcaaca
      781 aatcgcaaca ctgacacaag aaaaaacaaa tgtagaaact cagttaacag atacacaaaa
      841 gaaattagac gaagcaactg ctgaaaactc aagcttgaaa caatatattc aaaagttaga
      901 aaaagcaaaa gatgaagtgg aagatacagc tgataaatct cagcaaattg ttgaagaaca
      961 tttgcgtaaa taatctttct taacaagtaa acctaaatta gtaaagaagc aacagtaatc
     1021 tatactgctg cctctttttt ttagtacatg tccttcatct tcgcaggaag tgccaatatg
     1081 attttaagaa aggtaataga caaaaagtca aaaagataaa acagagtttc ttctttacaa
     1141 agggaatgta tgttcgtata atggtattga ggtgaaggtt atggagaaaa aatatggaac
     1201 agtagataag atccgtactt tgcaagttag tgccagaccg ttgatccgtt tcagtttaaa
     1261 tggggtcaat tgtttgattg cttcccatag tttgaacttc ctagctgaag tagacgaagg
     1321 gatgaagtta gtcgttacag gttattataa taatagaaag caatttgttg tgagatccta
     1381 tcatgtgtta gggaaaccca aaattgtggt agaatatgag aaaagtctat acccaagaaa
     1441 aaaggtccga caagatgaaa gaataaacta gaaaacaagc ggctgagaca aaaatgtcct
     1501 agccgcttgt tttggaaaga tggatgcaga cgatgggatt tgaacccaca cgagcatacg
     1561 ctcacaagaa cctcaatcta tatataacta catgtattta agtatttgtg atgaatcact
     1621 aaactttcca aactcatttg agggatattg cttaaagatt gaacagatca attcgaagcc
     1681 ctattagtat tcgctcaatg gggggatcag aggccataaa aagagactag tcattactag
     1741 tctcaggtag tggaattgta ggattttaaa cgttcctgga gagtaccggt atgtaactcg
     1801 aagaggtgat tatcatagtc gtaaaagtaa attgaagaag cctcgccttt cacacgagaa
     1861 cgtcctggtt cgacagttaa acctaacatt tggatttttg agacaaacga atctatatct
     1921 tgttcgtcaa ttttaaaagc aatatgattg tatgtctttg gcagtttatt cgacgaattc
     1981 tgcattactg caatccatag gtccttaact aagaaaaatt tttctggata taatgaaaat
     2041 ttttttgttg actatcgtag atttcttttg catcaaataa ggtatttaac aattaagtat
     2101 ttaaaagata acttttaagt tatctttaag taatgcttaa agctatgtta tactctatta
     2161 ggaaatataa gaaggagtat aaaaatgaac aaacataata gaaaaataac gaataacaaa
     2221 gaactgatct tgttactaat agggatttgg ggtatctttt tactctttaa tatgtatatg
     2281 ccgactatga gagcggatga cgtagtttat gcaagtagat tagatgaact tggctatttg
     2341 ggtgcttcga tagaacatta taagacatgg agttcaagaa ttattataga actattctta
     2401 atgttctttt ctaaacactt tatgctatgg aaattattaa attctacgat tatgttagga
     2461 attgtagtat tactttgcaa gtatgttttt gagaaaataa atgttaaaaa tttattattg
     2521 gtttgctcta tatactgttt aataccattg acagtaatgg gggaaaccgg ttggagagca
     2581 accaccttga actatcagtg gccagtagca tttagtttac taacttttta tccgtttttt
     2641 caactcttaa gaggagaaga aataaataga aaaatatatt gggtaagtat tcctttattg
     2701 atatttttaa ctaatcaaga acaagtcaac gcctgttttt ttgttctaac aagtatagtt
     2761 agtttatatt tgattgtcaa tggacgatat aattataagt tatcagtatt ttcaataata
     2821 agcttagcag aattaatttt ctctttgaca actccaggga atgcattacg tgctgcacat
     2881 gaaattaata aatggtttcc tgaatataaa aatttcaact ttcttaataa gttagattta
     2941 ggtatttcat catttggaaa gccgtttttt ttagacatga atatcttatt tttattgcta
     3001 tttttcttaa ttttccttct aacatatagg aaatgtcaaa actattatgt gcgtatattg
     3061 actgcattac cgtttttctt aaacctgatt atttattttg gtaatacaat gggtcaaagc
     3121 tttacttacg tcaatggaaa taaaagggca atgatttgga gtagtagtaa cctgaataat
     3181 ctctttacag agttaggaac gaaactgtca cttttttatc caggtacttg gatagctact
     3241 ttagtagtct tggcattatt actatgttta attgttggaa tttatttaag ttttgacaat
     3301 aagaaaactt ctattttttt agttattttg atgataatgg gattttgttc aaggttaatt
     3361 atgggatttt ctcccacagt atgggcttcc ggaatgcgga cgtactatat actttatgtt
     3421 gttatcgcca tcttagtact aatggcagtt aaagagctaa tgaaaagcat gagtgtacaa
     3481 aaaaatgaat ttatgcaatt tggattaacc gtgcttggta tttgtacctt tataataacg
     3541 gtgataaaca gataatatac aaaaaatccc tcgctttagt attaggtgag ggattttact
     3601 cgtttttcga ttaattgttt taacacttgc aagtttctac gagtagcttg tttccgacct
     3661 tcatatactc acctcaggct gtttacatga ttgtacgttc tttttgtaaa ggatacagtg
     3721 atatgtatat aatggcataa agaaacgttc aaacaaaaaa actcgtgaag caaaattaac
     3781 ttcacgagtt gtagtgcaga tgatgggact cgaacccaca cgagctaacg ctcacatgac
     3841 cctcaatcat gcttgtctgc caattccaac acatctgcgt aataataaca cactaatgat
     3901 agcgaggttt tacatagttg tcaagctcaa aaatgggagg acttattaag atggagaaat
     3961 atgacggaga attttcagga ctgggaatga tcttaggtgt attgattgga cttgcattcg
     4021 gacgtttttt gcttggtttt atgctaggaa tcatttgtgg aattgcaatg gattgggcag
     4081 cgaatctatg gaatgactat catgacaatt aagttctcaa cgcttgtttg tgttttttta
     4141 atacttacgt ttttcctaaa taagtggcaa aattttctag gatggagtat gatattgaaa
     4201 tagtaaataa caaaaaattt ggaaagttct aactttttga aatgagggat tggctaagtg
     4261 atgagagaat ttgaaaaatc caacaaatta gaaggtgtca gttacgatgt tcgcggtccc
     4321 gttctagaag aagcggagcg gatgcaagaa gagggcatta gtattttaaa attgaacacg
     4381 ggaaatcccg caccttttgg atttgaagca ccaaacgaag tcattcgtga tttgatcatg
     4441 aatgcccgtg actcagaagg ctactcagat tccaagggga tcttttctgc tagaaaagca
     4501 attgagcaat attgccaact gaaacatttt ccgaatgtga cgatcaatga tatctacacc
     4561 ggcaacggag tgagcgaatt gatcacgatg tgtatgcagg ggttgctgga caatggagat
     4621 gaagtactgg ttccaatgcc ggactatccg ttgtggacgg cttctattgc attagctgga
     4681 ggcacacctg ttcattatat ttgcgatgaa gaggcagaat ggtatcctga tattgatgat
     4741 atcaaatcaa aaataacgag tcggacaaaa gctattgtta tcattaatcc taataatcct
     4801 actggggcac tttatccaaa agaattatta gaagaaatcg ttgaagtagc tcgacaaaat
     4861 aacctgatta tttattccga cgaaatctat gatcgcttag tgatggatgg tctagaacat
     4921 attccgattg ctacattagc gcctgatttg ttcgttgtta cgctgaacgg tctatccaaa
     4981 tctcatcgtg tagcaggatt tcgagtaggc tggatggtcc taagtggaga taagtcacac
     5041 gtcaaaggct atatagaagg gctgaatatg ttgtcctcca tgcgtttgtg ttccaatgta
     5101 ttgtctcagc aaattatcca aaccgcgcta ggcggttatc aaagtgtcga tgagctgctc
     5161 ctgccaggcg gaaggatcta tgaacaaaga gaatatatct ataatgcaat caatgatatt
     5221 cctggtcttt cagccgtaaa accaaaagct gctttttata tattcccaaa aattgataca
     5281 aagcgattca atattttgga tgatgaaaaa ttcgttttgg actttttaca tgaacatcat
     5341 attctacttg tacatggtgg tggatttaat tggaaccagc cagatcattt tcgaatcgtt
     5401 tatcttccta aaatggatga tttgaaacaa acagctaaga aaatgagaga attcctagca
     5461 acctatcgcc aaaaatgatt ttaaaaacca aatttttcag taggagggcc acagacaaac
     5521 acactgaggc tctctttcta gtggagtttt cttaaataac taaagtatat tatttagttg
     5581 caagtctttt cttgaagttt gcgacttcct aaagtaaaat acgaatagaa tgcgaatacg
     5641 catattttgg ggaggatact tatggaaacg ttaatcgagt cctttggaac gatcattaaa
     5701 gagattcgaa aagaacaaaa aatgacgcaa aaaatgcttt cgcatgatat ttgttcacaa
     5761 agtgtattaa gtcggatcga aaataatgag gaactgccga atgtattggt gatgatgcag
     5821 atttgtcaac ggctaggggt aacgattgat catgtgatga atctctctcg aaaaactact
     5881 cggacaaacg ataaattatt tgaactgttg gatagttgtt tccaaaaacg tgattatcgg
     5941 aagttagaac aagtactaaa atcttctgat atttctgaaa atctgtatca agcaactgac
     6001 tttcaacgct attattatta tctcggtgtt tgcgaattca cgttgcgtca aaatatctct
     6061 cagtcccttt attatttgaa agaagcatta agctactcta gccaaaaaga aagaatccat
     6121 gtttctgaca cagaaataca actgattagt tgtatcggaa aaatatatgg tgtggcagga
     6181 aaaacggctg aagcacgcta ctatttgagt cgaagcatcc aactgctgca tcaatcagct
     6241 gatgaacgat caaaaagtga attttcgcaa attttttata attatggaaa tttcttattc
     6301 catcaaaatg aaatcgacga agcgttagaa caggtgaatc aaggaatcta ttgggctcaa
     6361 gagaagaata gctactatta cctcaatgat ttatttgtgc ttaaaagctt aatatacaag
     6421 cgtaaagaag aaccagaaag agcagcattc tatgaaggct tagcacaagc tgtgaaacaa
     6481 atttcaaaaa atttgtaaat ggtgtagacc attcttgact tcttctctaa ataagagtac
     6541 tttagaggta gaaaaacaaa gggagagaat agaatgagaa cttttatcta tgctgacaaa
     6601 ttctttttag cgtcaggtgt caaacatgca ggctatcttg aaattaccga tggacttttt
     6661 ggtgaataca ccaaagaact acctgaggga gaagtcaaca tcattgacca aagcggcaaa
     6721 tggattgctc ctggtttggt agatacacat attcacggct atatgaatca tgatgtaatg
     6781 gataatgatg cagaaggcat caaagtgatg tctgaaggtc tgttatcttg tggagtgact
     6841 tcatttttac caacaacatt gacgtcaagc aaagaacgtt taaaagatgt agctgagaca
     6901 atcggcaaaa tgtatcagga agcaccagga gctaaaatca aaggaatcta ttttgaaggt
     6961 cctttcttca cagaagaaca caaaggggca caaaacccaa gttatttcgg ggatcctgat
     7021 ttggatactt tccatgaatg gcaagaagct tcaggtggtt taatcaaaaa aattgcttta
     7081 gcaccggaac gtcaaggtgt agaagagttt gtcaaacaag tgactgaaga aggagtagtt
     7141 gtttctctag gacacagtaa tgctacacta gaagaagcgc aaaaagctgt tgaagcaggc
     7201 gcaagtgtat ttgtccatgc tttcaatggt atgcgtggat tgaatcatcg cgaaccaggt
     7261 atggtgggtg cgttattatc tttagaacat gtcttttctg aattgatttg tgatggccat
     7321 catgtacatc caaatgctgc gggaatccta atggaaaaag caggacatga tcatgtcgca
     7381 ttaattacag actgtatgat ggccggtggg atgcctgatg gaaattacaa tttaggcgaa
     7441 tttccagtag tagtaaaaga aggaacagca cgtctagaat ctggcaactt agctggaagt
     7501 atattgaaat tgaaagaagc aattaaaaat gttgtagact ggggattagc aacaccagaa
     7561 caagcagtca tgatggctag tctgattcca gcaatcagct gtaaaatcga tgatacatgt
     7621 ggaaaaatcg ctcaaggacg cgatgcagac tttatcgtat tgaaccctga tatgacattg
     7681 gaagcaactt atctagatgg cgtggaacgt ttccacgcat aatataattt aagttaacct
     7741 taaaactaaa cttcttaagt ttggttttaa ggtttttttg tgttctagta aaaatcactt
     7801 tacataagtt tcaaaagttt ctttaatttc ctattgttca ataagtaaca atgtaaagat
     7861 aaaattaaaa atgttaatac gtattagcaa aataaaagct ttttttattg tgaataaatg
     7921 aacataatta ggaggagaaa gatgaactta tttgacattt ttcgatcgac tttgacagat
     7981 atgtcgattg tcagtgcaat cacttcaaca gtttttatta tattattagg gtttgtttgt
     8041 agaaaaaagg ggattttttc tgaatctgtt ggaaaaattc tatcgaaagt cgtcttaaca
     8101 gtggctttgc cagcactggc atttcgagct ttcatgcaag atatcaatac agaattactg
     8161 atgcaaggaa tcaatgtctt gatttgggga ttagttattt atattattct tattctagtt
     8221 tcaaaacctt ttttcttaag ctataaaggg gataagcaaa caacgttgcg tgtcctgacg
     8281 atctttggtt ctacgacctt tttcggaacg cctatcgtca gtgcgattta tggtcctgtc
     8341 ggcgtgatgt attcctcaat tttcaatatt ggctaccgaa tctttttgta ttcttatgga
     8401 tacataaaaa tgagtggctt gaagatggaa gctaaaaata tcaaagcgat gttcgtcaat
     8461 ccaatcatta tcgctacttt tgccggcctt ttcatttggc tgttccaagg gtatctgcca
     8521 caagtgtcag ttacaggtgc agaaggagag ataagccaag tagcgttttt gcgaattgac
     8581 cagactgcac cgtggctatt tcagccgatg gattatttag ctggtttaag ttcaccattg
     8641 gcgtggctag caatcggcgc aacattaggt gaagtaagct tcaaaaaagc tgcatctgaa
     8701 aaaacttcat ggtattacag tctagtcaaa gtggtatttg tacctgcatt gaatatcgtg
     8761 attctagcag ttttgacggc aacgaatatt ctaccagtca attttgaagc actggcaaca
     8821 atcgttatca tgatggctac accagctgca acagtggcag cagcctatgc tatcagtttt
     8881 gatagggagg cacttttagc ctccaatgca tccttactgt caactatttt tgctgtggta
     8941 ctgacacctg tatggatcgt tgtattacaa atgattcatg gcataggttt attttcataa
     9001 gaaatggagg aagaaagtat gagaatcgca tgttacggtg ttcgtccgaa cgagatagat
     9061 ttttttgaaa aattgaatat ttaccattat gatctcagtc tttatgaaga actgttgaca
     9121 catgacaata tcgtgtcagc aaaggaccat gatgctgtat tgttgagggg aaattgtata
     9181 gcagatgaga ccaatcttgc aaaaatgcag gaatatggga ttcgttatgt ttttacacgg
     9241 actgtgggag tcaatcatat cgatctacaa gcggcagctg attttggtat gacagtagcc
     9301 cgtgtaccat cgtattcgcc aaatgcgata gcagagttat ctttgacttt tgccatgatg
     9361 cttttgagaa atactgcgta tacgacgatc cgaacatctt tcaaagattt tcgagtggat
     9421 gagcaaatgt ttagtcgtga aatcagaaat tgcacagtag ggatcatagg aacaggcagg
     9481 atagggttga cggaagctgt tttgtttaag ggactaggag ccaaagtgat cggctacgat
     9541 attcatcaga cagaagctgc taaaaaagta ttgacgttcc actcgcttga agagttatta
     9601 gccaaaagcg atattgtcag tattcatgta cctcatatcc caggtgaaaa cgatcaaatg
     9661 atcaatgaag gatttttagc tcaaatgaaa aaaggtagca tcttgattaa tacagcccgt
     9721 ggagagcttc aagataatga agctatttta agagctttga aaagcaatca tttagctggt
     9781 tttgcaaccg atgtttttgc aaatgaaaca gaggtttttt tccgatcctt caagccatgg
     9841 gagacgattc cagaccctgc tatccagcaa ttagtagaaa tgtaccctag agttcttgta
     9901 accccgcatg taggttcaaa tacagatgag gcattatcaa atatgatttc gacaagtttc
     9961 gagaactttc gtgaaataat agaaactgga aaaacgaaaa atgaggtatc tcttcctaaa
    10021 gcccgtcaat taagataaga aaacatggat attcgtcaat aaaatgttta aaaatagtcc
    10081 aagtaaacag ttgttacttc tgtatacttg gactattttg tatgggataa ttcgatgact
    10141 caacagtaat ctgtgagctt tttccgccca ttctttgtca attcataaca ggttagcggc
    10201 tggccgattt ccaaatattt gattctttcg ttcaatatat cttgttctac taaaaaattc
    10261 aaatatttct tgacggatat acgtgacagt gagctttttt gagccagttc ttttgttgaa
    10321 aaaatagtat cttcttttag aataaatgca cttatttttt ttaaagtcag ttttgacaga
    10381 cctttaggca aatcgatgaa tgatggtatt tcgtgaatgg gagtggtgtg gttgaatact
    10441 tgatcgatca atttctgatc ggctttttca gtcgtttgga ttgtctgaga gaaagcaaga
    10501 tacttttcga tcgccagctg aaaacgttgc aaagtgaacg gtttgatcaa ataatctaag
    10561 gcaccgtagc ggatcacatt ctttatggtt tcgatatcat ctgcagcagt gatcaaaatc
    10621 actgggatat catttccttg ctgtctaagc caagataaaa aatggatacc actttcgcca
    10681 ggcatgtata catcaagcag cagcagatct gcgttttttt gtctgaaata tttttttcct
    10741 tcttctacat ttctgaaact ttcgattacg ttcatattag ggatgctttc cacaaaagta
    10801 cgattcaatt ctgcaaccat tggatcatct tcgattatca ggacatttgt catcattttg
    10861 atcttccttt tttggtaatt ctagatagac gattgtaccg ccatttgttt gtttggaaaa
    10921 ttctaaaaat ccaccgtggc gtttgacgat tgaatggatg agattcaatc caaacccacg
    10981 attgattccc ttcgtcgaaa aacctagcgt aaatattttt tctgagattt cttctgatat
    11041 cccgacaccg ccatcgcaga cggtcacgat gaatacttca gcttctgtgt catagtttaa
    11101 atgaatccgg acctgtccgt ttctttcatg tttttgtagc acggcttctt ttgcattatc
    11161 aagtaagttc cctaacactt gcaaaagtga atggatggag tcatccatct ccattttagg
    11221 gataaaagaa tcgctttcta acagcatttc aacagattgt tctttggctt cgtttatttt
    11281 gcctaataaa aaaccggcaa ttgcaggtac tttgatcaga cttgagactt ggccaatctc
    11341 ttttttataa ttttgattta gctgctggat aaaatgggtg acttcttcat acttcttcaa
    11401 atcaatcaaa ccagaaatga catgaagttt attcatgaat tcatgggttt gagctctcat
    11461 ggagtttata tattgtttcg tccccccaag ctcctccatt aatttcatta cttctgatcg
    11521 atttcgtaaa gttgcgactg ctcctaaaaa acggtcattg ctaaaaagag gtgtaacgct
    11581 agaaatagtt tctatcccat ttaaatacat ggattgagtc cgaacggtcg tttgatgctc
    11641 aatcgtctct ttaaacaaaa cgtcatatag attatcactg atttttttcc tacttcgaaa
    11701 tccaatagct gctctgtttg gtagaaaatg ttttttgcag gtgtattgat caagaggata
    11761 gttttatcag aagagatggc taagattcct tcatctactt cattttcgat aatcgttttt
    11821 tcacttaact gttctgctat ttcttgtggt tcaagtccaa gcagtacttt tttcattttt
    11881 tgggcaagaa aaacggcgcc tatgatgcct acgctaagtc caataatcaa tgaaaaaaca
    11941 atcatttttt gtccatcttt gacttgattg tcaatcgtgt tgtttgttaa gccaacacaa
    12001 actgcaccaa taatctcttg atcataataa atagggacga aatagcggaa gccgaaccct
    12061 aacattcctt ctttttcaga gaaaaattct tgtcctccaa tcactttttt cacatcttca
    12121 tgacttgaga aaggaagacc gatgtaactt gggtctgcgt gagaatatcg aatgccttca
    12181 gggtccatga caacgataaa atctaaattc gatgcaatca tgatttcatt tgttattcct
    12241 tgaatcgcat gtgtcgggtc accagtctgc agcttttttt ggatgagttt atcattggca
    12301 acgattttag caatgctaga gattttatct gacacactag atcggttcat cgaaagaata
    12361 aaattcctga tcaatatggc agaaacaatc aaagaaacga atgtggagaa acaaacgaca
    12421 aggatgatcg tgttctggag ctttaatggt tttttcttaa aaaaaggaaa cataggctac
    12481 ctcgaaagaa tttattgtga aaattttaac atgaaaacaa aaatttaaca aattgtcaac
    12541 gataaacaca accttcctat aaaaaacacc actgagtcgg taaaagaagc tatacagaat
    12601 tttcaaacat tggtatgata gacgtagaaa tggaaggagg cgtgtatatg atagggggaa
    12661 tcgcacttga gacgatttat tggtatacat tgctgatttg tgcaggattg gcagtcctat
    12721 tgattatttt tggagatgta ttcgattttg atggcccgct tgatccgatg cttattatcc
    12781 cttggttaac tttcacaagc ttatttggtt ttctaggtga gcgtttaaca gatcaatcat
    12841 ctttattttt atttattgct agtggaggca tatcgatagt gttggtattt ttgcttaatt
    12901 tctatatatt gatgccgatg aaacatgccg aatcaacgat gtcgtcttct gaaaaaactc
    12961 tggaaggaca agtagctgtt gtgatcacac cgatccctgt acgaggtatg ggagaaattc
    13021 agttgaaaag tgttaccggt tccattagtc gtccagcttg tttttacagc ccccaagaac
    13081 agacagctga gcggggaacg aaagtattga tcattgagat caaggaaaga atctgttatg
    13141 tcatgcctta tgaaagcatg ataaaagctt aaaggagcgt gtgttaaatg gaaataccag
    13201 ttgtttttat cgcgatcgca atcgcagctt tgattttact gatgttgctg atcgtctttg
    13261 tagcgaagta ccagacagca aaaccggatg aagcgttgat catcagtgga agttatttag
    13321 gaagcaaaaa tgtgcacatt gatgaaagca ataacaaaat caaaattgtg cgtggaggtg
    13381 gcgcattcgt cttgccagtt tttcaacgtt cgaatcggat cagtctatta tcgagtaaat
    13441 tggacgtctc cacccctgaa gtgtataccg aacaaggcgt accggtgatg tgtgatggta
    13501 cttcgatcat caaaatcggt tcatctgtag aagagattgc tacagcagca gaacagtttt
    13561 taggcaagac aagagaagaa ctggaaaatg aagcaagaga agtattagaa ggacacttgc
    13621 gctcgattct tggttctatg actgtagaag aaatctatca aaatcgcgat aagttcagtc
    13681 aaagcgtaca agaagtcgct tctgtagacc ttgcaaaaat ggggttgatc atcgtttctt
    13741 tcactatcaa agaggtcaga gacaaaaacg gttatcttga ctctttaggg aaaccaagaa
    13801 tcgcccaagt caaaagagat gcagatatag cagaagcgga agctctaaaa gaaacgcgta
    13861 tcaaaaaagc tcaagcagaa aaagaatcac aagcagctga attacagcga caaacagaga
    13921 tcgcagaatc attaaaagaa aaagaactga aattagcaac ctacaaacaa gaacaagatg
    13981 ttgctaaagc aaaagcagac caagcctaca atttagaaag tgcccgagca caacaacaag
    14041 taatcgaaca ggaaatgcag attaaaatcg tagaacgcca aaaacaaatc gaactggaag
    14101 aaaaagaaat cacacgtcgt gaaaagcaat atgattcgga agtgaagaaa aaagcagatg
    14161 ccgatcggta cgctaaagaa caagaagcac aagctcaaaa agtcaaagaa gtgactgaag
    14221 ccgaagcaga acgattccgt gtggaagcac tggcagaagc ggaagccaat aagacacgtt
    14281 tagctggaca agcagaagcc gaagcagcac tagcaaaagg aaaagcagaa gccgaagcaa
    14341 aacaaaagat cgccaatgcc ttcaaggaat atggcgaagc agctgtcttg agtatggtta
    14401 tcgatatgct accgcaattg atgcgcgaag cagcacagcc gctaggaaat atcgaaaaaa
    14461 tctctgttgt cgatacagga agcagttcag gagaaaccag tggagcgaac cgagtgacaa
    14521 attatgccac aaatttgctt tcttctactc aagaaacact aaaagcgaca acaggacttg
    14581 acttgaagga aatcgtagag aatttgtcga cacgtgggac tactagccaa gtaaatatgt
    14641 atgaagcgcc aaaagaggca gaacaaaact cgatgaagga agaataaagt agtacacttc
    14701 accgttcaat caaaaatgga ggaagacttt ttaaagttct tctctccatt tttttatttt
    14761 attgcaaaag aaaattagtc ctcgctattt ttcttttgtt tttggatctt agcttctatc
    14821 tcttgtaaat ttgttcttag caatcgttct ttcttttgga gttcgaccaa atcttctgat
    14881 agttcttcct tcttttctgt ttcaggagga cgaagaaaga aattgatacc gattgcaatg
    14941 aatgtcccta taaaagtatc tagtacacgt tggatagcat acaaagcgga ttcgccttga
    15001 gggatgctca acgcaatcag caacaacgta gctattgcgg aaatgatacc ggaattattg
    15061 ttaataccat ctgacacgac gatcacaata ataactagaa taggaagcaa gatcaattct
    15121 accaaaaaat cattgtgaaa atagcttttg acaaaaaaat aaatgatagc taatgtacca
    15181 cctaatgtat tgccgatgat cctagacttt ccaaaagaaa cggttgtagt caagtcctga
    15241 cgcaaagaaa aaacagcaga taatgcagca atcatcggga ttcctcgttg agtcaaatga
    15301 aaaaacaaga tacataaaaa aacagctaag gcacttttga tcgtacgcat acctagtcga
    15361 aaacggccaa attgcataga gattctcctt tttggtcttt ttactatcat acctaaactt
    15421 ttgtctgata gctagttttt cattattcaa gtataattaa aataaaaaag ggggaagtaa
    15481 atgcgtatag gaatgcgaac agtcaaaaca gtgatcagtg tcttcttttc gatggtcaca
    15541 gcgggtagct tatcgctgtt gtattggcca gctgcaggga tcattgcatt attaagtgta
    15601 ggaaatacca agcgctctac attgcgtacg ggcatgaatc gaatcgcagc ctttatatta
    15661 gctacggcaa cagcactgtt cagttttttt gctgttgggt atactatttt tggcttcagt
    15721 ctttttttat tcctgttcat cccgcttgcc tctcgcttca aattaaccga agggatagta
    15781 gtaaattcgg ttttggtcac tcattattta gtggaggaaa acatgtcttg gcagctgatc
    15841 ggcaatgaag cgggattaat ggcgattgga ttagtatttg ctttgcttgc aaatgtttac
    15901 atgccagatc gtacgaagca gctgaaggaa aaccaaatac agatcgaaaa agaatttcga
    15961 aatattttga gacaaatggc agaatttctt ttatcagaaa ataaagaaga tgtgcagatc
    16021 aaatgcgaac atttactgac attcattcgc gaaagccaag aagatgccag agaacatcaa
    16081 gaaaactact ggctcagaca gccgctatac tacgaaactt acttctctat gagaagagca
    16141 caggccaatg tgattaaaga catgctggaa aatctagaga gaatccagca accagcttat
    16201 tatggaaaac atatctatgg tttattgatt tatactgcag aaacattttc agagtcaaat
    16261 gatggcagac aaattttaac acgtattgaa gaagtttatg tattgtatcg gcaaatgcct
    16321 ctgccgacta gtcgccctga attcgaggat cgagcagaat tgttccagtt tttgcagtct
    16381 tttaaaagtt ttattgaaat caaggcagaa ttctcccaac aaaaaatcca aaaatagagg
    16441 ttgtgaattc agctaatatt caggtcaaaa agctgatttc acaacctctt ctttaaacgg
    16501 tgttctcctg tcaactcctc ggtcacaagt cacaatattc aacaaacatg agaagctgtt
    16561 ttttgaaatt gctccttgtg actcggagct gaacggtagg ctcacaacct ctttcttaaa
    16621 cggtgttcaa aagctttcct ctgcggtaat aagtccagcc aaacgaaaaa taggaggagc
    16681 tatttttgtt tggctgttct tattacttga tgcagaacag ctttttcaca accttttaaa
    16741 attacatatt tttccataga acttgcattt gatccaagat ttcgatgaca tcttttgtca
    16801 agaagagaga gcgattgggc aagtcacctt caatcatgtg taccatattc tttatttcgt
    16861 aattcattgc ttcagatgta ttcccgcttt cgatgaattc ctttgtacca tctgtaaata
    16921 agatctccgc acgatcagca cgcgggtaat cagcaatcgt cacataaccg ttctcaaagg
    16981 caacgatacc gacttttggc atttttgcct gaaaagtcaa gctgacagca gcaagttcat
    17041 tttctttgtt acgcaagata gtgacggatt gttcgtccac gcctgtttca aaaggaacca
    17101 ttgtagaggc aactacttca ggttgagagc tcaaaaagaa acgagcgaaa gatacagcat
    17161 acgtaccgat atccaacaga gctcctccag ctaattctgg attgaagaac cggtttttag
    17221 ggtctggttc tttgtaactg ccgaaaggtg cttggatcat ttttaaagcc cccagttttc
    17281 ctgtatccat tattgagcgt aactgctgat aaagtggcat gttgaagatt gtcatcgctt
    17341 ctgctaagat caaattcttt tcttctgcta agcgcatagc gtcagccagt tcttttttat
    17401 tcatcgtgat ggctttttca catagaacgt gtttgcctgc ttccaatgct gccaagatat
    17461 ggtcgatatg ctgtctattt ggtacagcaa tgtacacgat gtcgatttca gcatcgtcta
    17521 acatttcttg atagcttccg taagcttttg gaatattgta acggtgagcg aagttttccg
    17581 ctttttctga agttctggaa gcaactgctg ttagttcgct cgtctcttga ttgaagcttt
    17641 cggcaaactc atgtgcgatg gtaccaagac caacgattcc ccattgatat tttttcatct
    17701 gaaaacgctc cttcatagtt ggattgagtt tcttgtctgt ctattattat aacaaaaatc
    17761 acgaagaata aagaggagag gacttgaaac attaaaaaaa atgaaagcag cgatttaaga
    17821 ggtgcttttt gatcaaaact taagtagaat agttaaaagg aggggataaa cgtgcatcaa
    17881 gtatatattg atcttattat gagtgtatta ttagaacagt ttgaaactga aaaaaatttt
    17941 ttagatgaat acttgctgat cagtccagaa caatggcaaa aatggaaaga tggcgagaaa
    18001 aatctgccat ctgaagcgat gcagaaagta aaaaatcttt tcactgatta tgaatggatg
    18061 ctaacgcaaa agattttgcg tcaaacgatt atttttcccg aaaaacgaaa cgttgccgtt
    18121 gcagagtaca agcaaatcaa aacacgcatc gcgcaaaaat ggcttagcag caaagcagca
    18181 aaagtcgagc tgatcccttt gaaaaataat cagcaggctg gtagatacct tgacttgaaa
    18241 gtaagtattc aatatgatga atggggctat gatgatattt tgaattttcg tctgccagca
    18301 gatgttcaaa aacagatcga aggagaaaaa atcgaacttc tcgattgggt gaatgaaaat
    18361 ctggaagaaa cttacgttca tgaggcgaat ggggaacgtg aagaacaacg aaaggatttt
    18421 tcaagtgaat aaatttttag gatttggagc agttgtgatt atgatcgcag caatgggcta
    18481 tgttattttt agcgatgatg attcgcatgt tcaagccggc aatcaagcag tcgaacgaac
    18541 aagcaccaaa acaactgcta gtaaagaaaa gaacagtcaa acaaccagca gcaaggtaaa
    18601 ggacaaagaa acaaaagatc gtacgtccaa acaattgcca gatgttaaaa cagacgattg
    18661 gaagctagtt ttggttggtc cagatcacaa attagaaaaa gaaattgatg agtctactca
    18721 attggcatca ttaagtaacg gatacttgat cgacaaacgc attgctccag aatatgaagc
    18781 atttgccaag gctgctgaag cagctggatt tccattagtg atggtctcag cttaccggtc
    18841 tgtatcatcc caacaacaag ttttcgaaca aaatgtccaa gatgtgatga gtcgtcaagg
    18901 tgtatcagaa gaggaggcga caaagattac taaagaaacg attacggagc caggatacag
    18961 cgaacaccac acaggtctgg cagtcgatgt cgtcgatcaa aactggtaca acagttatcc
    19021 ttctactgta ttggatgcaa gttacggaga tcagcctgga gctaaatgga tcgcagataa
    19081 tgctgcgcag tatggattta tcgttcgtta tccaaaagga cacgaagata taactaagat
    19141 cacatatgaa ccttggcact tacgttatgt cggtaaagaa aacgctgagt atatcaccaa
    19201 acaccaattg acgctagaag aatatctgaa tgaattaaat gaaaagtagg aattgatcat
    19261 ggcgaaaaaa aagtggaaaa agcaaaagcg caagaatatc cgttggccag cagtggttgc
    19321 cggtgtagca gtgatactct tagcctttgt tttttccttg aagaatctgt cttctccttt
    19381 tactgataat cagcaagaaa atgaagcgca atcccatcag caatttattg aacggcttgt
    19441 tccgcatgcc caagaactac aagatggtta tggagtgctt cctagtatca ttttaggaca
    19501 agctatatta gaatcaaatt ggggaaaaag taccttggct agcaaataca ataatctttt
    19561 cgggatcaag gcttatgggg atcagaagaa agtaagcctg gaaacaaagg aatttgttaa
    19621 cgaagaatgg ataactatcc aaggagactt caaagtgtat gattcatggg aacagtccat
    19681 ggatgaccat acacaactgt tcgtccaagg agtagattgg aatcctgcgc tgtatgaaaa
    19741 agtgattacg gccaccaact atcaggaagc cgcacaagca ttgcaagatg caggttatgc
    19801 gacagacccc ggttatgcgc aaaaaatcat ccaggtcatc gagacgtatc aattgaatca
    19861 gtatgaccgg agatagcaaa ggagaagtgg ctgatggcag aacaatttac gccagaaatc
    19921 atgacggaat tgagaaaaga tatcgtaaga gtaccggaaa tcattttaga agcaagcggg
    19981 atcaagatat ttggaaaatt gatccgttcg atcatcttta ctacggatat tgcgatcata
    20041 cgtaatacaa atgcgaatgc agtaattgct gtgtatcctt ttacacctca tccagcgatt
    20101 actaaaagca ttattgaggc agctgacatt ccagttttct ccggtgtagg tggtggtttg
    20161 acacaaggtg ctcgttcttc ctacatgagc atgttcgcag aagcgcaagg ttcgttaggg
    20221 gtcgttttga acggtcctac gcctttagag acagtaaaag aagtttgtcg agtagttgat
    20281 attccagtta tcagtacggt aacgtcgaaa tatacagaaa tagatgaaaa gttagcagct
    20341 ggtatttcga ttatcaatat cagtgcaggt aaagaaactg cagaagcagt acgccatttt
    20401 cgtaaaaaat atcctgaact tcctatcatt gcgacaggtg gtccaacgga agaaagtatt
    20461 gctgagacca ttcaagcagg agcaaatgcc atcacatata cgccaccaag tagcggagag
    20521 ctttttagtc gtaaaatgga aaaatatcgg gaaactgaga aaaagaatca tgaataacag
    20581 gtgagaatca aaagacgagg aagagcagat agtggctctt cctcgtcttt tgtactccag
    20641 tactatttgt tcagaattca aaaaaaagag aaattttttt gtttttttac ctgtaatgta
    20701 cataagaaga gttaaaacct tttgttatta ttagcattct aacaaaaaaa tgtcttgatt
    20761 tattgaagcg ctttctatag aatgaaaaac gaataaaaac taaaaaaggc acccgacaat
    20821 ttagtagcag ctaaattgtc ggggcttgaa aagtcagcgg gaactgacca atcaagttgc
    20881 catagtcaat gcttgtgcat cgacgtgtat attgtaccta atttcataca gaattactag
    20941 ttatttttct gttttttagg gaagagcgca cacgaagaag catgcttatt tagctatgtc
    21001 aaggatattg ggcagttgtc tgatatcctt ttttctttgc gtaattatcc tcccatcatt
    21061 gaagcaagaa gtgtttttag cgatgaaaga agaatcagca ctatctaatt tttcttgaaa
    21121 gtattgagtt gttcatccta ggcagaacga taaggcaagt cttggttttt attccccttg
    21181 aaaaagatcg tttaataaat atgttctgta taaaaataca gtatattgaa aaataacaat
    21241 ggaagtagag gtgcagagaa tgaagaaaag aatgagaatc atcttagctt taagtgttat
    21301 gttattcttc ttgacggctt gtgggcaaga agatgacaaa aaaatcacgc tgaacatggc
    21361 ttggtggggt tcgcaagtcc gacacgatgc aacagtaaaa gtaatcgagc tttatgaaga
    21421 aaaaaatccc catgtgacga tcgattatga attttatgat tatgaaggat attacacaaa
    21481 attaggtaca ctagcagcat cagatgatat ctgggacatt ttccaactga acaatcaatt
    21541 tcctcaatac atcaatcaga ttgaaccatt agatgaatat gtggagcaag ggataattga
    21601 tatttctaat gcagatgaat cctatttagc aacgacgact tacgacgggc aattagttgg
    21661 actgtctaac ggggtgaatt cgtttgcgat tgcttacaat aaaaatatat ttgaacaatt
    21721 aaatatccct gaaccagctt caaattggac atgggatgaa tttgaacaaa tcagctatca
    21781 aatcactgaa gaattaggaa tctatgctat ttcaagattc gaagatttta tagctggctg
    21841 tattattcaa ataccacaag ttgagaaagg attgaatttt tataatcggg aagatctatc
    21901 taacagcttg ggatttgaaa atccagacta tctaacagat tttttccagc tgcgtaaaaa
    21961 tctggtagac aaaggggccc atccagaacc aggtggtgga gctgcaagta cgacggatct
    22021 gtcttttcag gcggttaggg atagagaagc ggcaatggtg tttctatcaa gtaaccagtt
    22081 gacagaaata ttgtcagggg ctcctgaaga tatggagatt cagttgatta atccacctcg
    22141 cagaaaagca gatggagaat ctggtatccc tttacgttct tcgcaaatgc tgagtatggc
    22201 aaaaagctca gcccataaag aagaagctgc taagtttatt gatttctttc aaaacagtga
    22261 agaagccaat gaaattttaa aaggagaacg tggtgtacca atcatgtcga atattcgaga
    22321 acagctcgct cgtgcagatg atgcaacttt gcaagcgaca tttgattatt tagaaatgat
    22381 ttccgatatg gacaacggcg aagtgaatgt ctttgatagt gctttgaatc cggaaattca
    22441 agaccaatac aatcttttag ttgaaaaagt catttatgaa gaagtaacag cagaaggaag
    22501 gcatgaaaaa agaaaaacta aaacggttct tggatcaaga caatgtggtc ggttacgtat
    22561 ttgcttctcc attcatccta ggatttctat tgataacagt gataccgatg atcttgtcac
    22621 tttattattc gttcaccgat tatcgtttgg gaaatccagt ctcatggatc ggcttggata
    22681 attatataaa gatgctgagt gatgcccgtt ttttgaactc catccgagtc acttttctct
    22741 atgtatttgc atctgtacct gtgaaactga tctttggtct attgattgct tatcttttaa
    22801 cgaggaatat caaaggaacg actttctttc gttctttgta ttatgtgcct agtttgatcg
    22861 gtggcagtat ttctatcgct ctagtttggc gggagttgtt ttctaaaaac gggttagtga
    22921 atattttcct ggaacgatta ggcgcacctg cgctttcctg gtttagcgat caaaaactgg
    22981 tcatgatccc tcttgttttg atgtctgctt ggcaattcgg ttcggcaatg atcatctttg
    23041 ctgcaggtat caaagagatc ccaggctcgt attatgaagc ggcagagatc gatggagcga
    23101 acaaatggca gacgttttac aagatcacgt tacctcttct ttcgccgatc attttgtata
    23161 acttggttat gcagacaatc gcggcgttta tgacttttac gcaggcattt atcattaccc
    23221 aaggtggtcc gaatgatgcc acgaactttt atgctcttta tgtgtataac caagcttttt
    23281 cttttaattc tatggggtat gctagtgcta tttcgtggat catgctgttg attatgggag
    23341 ctgtcacctt tctgattttc aaatcatcaa aaaaatgggt cgttgtgtga atgagaggag
    23401 gaagaaatga ataatacagt aacgaaatgg tttcaatacg gttttatttt gctattaggt
    23461 ctagtcatga tttatccggt cttatggatg atcgcaggtt cttttaaaac ggatcaagag
    23521 attatgagtg gttcgctgaa tttgattcca gagacttttc aatgggaaaa ttacgcaaaa
    23581 ggatgggcgg ggtttgctgg aatttcattt tttactttct ttaggaattc gtttgtcatc
    23641 tctgcagttt cgacgatagg gacagttctg agctcgactt gtattgctta cgcattatcg
    23701 agaatcgatt tcaaaggaaa aagattctgg ttcgtcgtca tgttagtaac gatgatgatt
    23761 cctgcacaag tgattttgat tccgcagttt attatttaca atcggctgaa tttggttggg
    23821 acgtatgtgc cgctgatttt accacatttt tttggacaag catttttcat ttaccagatc
    23881 atgcaattca tggtcagcat ccccaaagag ttagatgaat cagcgattat tgacggatgc
    23941 agcaagtata gtgtctttac caaaattatt tttcctttgc tgaaaccttc gatcatcacg
    24001 acgatcatca tccagtttta ttggaaatgg gacgacttca tgggaccatt gatttattta
    24061 aacaagccga gatcttacac ggtatcgatt gccatcaaat tatttgcaga cgctggatct
    24121 agtacgactt acgcgagtat gtttgcgatg tctacgttgt cgctgctacc ggtttttctg
    24181 attttcttat tcttcaataa atatcttgtc caggggatca gtacaagcgg tctgaagggc
    24241 tgaatatgaa aaagccaata gaagagatgt ttctttacaa gctcaactac tatgtctgtc
    24301 ggattttttt aaccagtctt atcagtaatg ctcttttcgc tttagttcta ggatgtttgt
    24361 tcactttagg attagtgctg tctgctcgtg ttgtctatct gatttatttt ttgatgatgt
    24421 atccttctta ttatctatct gtccattact tggcagaaaa aaaggtatgt tggaggaaag
    24481 ggaaaatcaa acggatgtgt ttcatcatta cgttgtttca gctgtttgtc attatttttg
    24541 cagttgattg gtatttatta gtgggaaaga ttggagcttg gtatctgatt ccttttctat
    24601 tgtgtctgtt gctttttgta tcccatagtt tttttagtac gctaattgat atctgcaaag
    24661 aagataaagc agatagtctt gtttatttga tcaaaaggaa tatctggctg ttgtcaaaaa
    24721 acgtcaaggg gtttctgtgg tatttatgcc ttttgtatgg actgttctgg tgtatacagc
    24781 ctgatttttt actcactgca ttactgtatc cagggctgcc atttgtcaca gtattttgct
    24841 taaaagaaaa ggagaatgac tttaaatgaa gaaaaaatat gtacaagtcg gacttggtgg
    24901 gagagcgcgt tttttttatg aggcaattgc ctctacgtat catcaaactt gtgaactagt
    24961 cggtttttgt gatgtatctc aaacaaggat ggattatgca aatcggatac ttgtgaacaa
    25021 gtatggacag aaaaaagtaa agacatgtat gccatcacaa tttgagcaac tactggatga
    25081 cgtacggccg gctgaagtca ttattacgtc agtagatcga acgcatcatg attatatcat
    25141 tcgtgcaatg aaaaaaggat atgatgttat ttgcgaaaaa cctctgacta tcgatgagga
    25201 aaaagctcaa gaaataataa cggtccaaaa acagacggga agaagcctgc gagtcacttt
    25261 taattatcgt tatgcaccgt atcacacgaa gataaaggaa ctgatagaaa gtgatgtgat
    25321 tggtgatgtg ttttctatcc acttcgaatg gctgttaaat acgcaacacg gggcagatta
    25381 tttcagaaga tggcatcgaa ataagcacaa tagcggcggg cttcttgttc ataaatctac
    25441 ccatcatttt gatctagtga atttttggtt gaacagccgt ccccaaaagg tatttgcatt
    25501 cggtggatta aacttttatg ggatgcataa tgcggagaaa cgcggagtag aaatatttta
    25561 cagtcgatgc tatggaagcg aactagctaa agctgatcca tttgctatag acctgtcagc
    25621 caatgaggaa ttgcgagaaa tgtatttaga agcagaaaaa gacagtggat atattcgtga
    25681 ccgcagtgta ttttccgatg atatctctat tgaagataca ctaggcgtaa cggtcaaata
    25741 tgaaactggt gcgattttga cgtattcgct aaacgcgtat atgccgtggg aaggattcaa
    25801 tgtttctttc aatggcagca aaggacggat cgaaatgaaa gtgatcgaaa agtcttacat
    25861 caatgcaggt ggagataaaa aggatgaagg agcagccgtt cttcagtcca tccatgtcta
    25921 tccgatgttc ggtgagccat atgaggtaat cgtcgaacaa gaagcgggcg gacatggcgg
    25981 aggagatccg cgtttattag atgatctatt tggtgaagaa aaagaagaag atccgtggaa
    26041 tcgggcagca actcacgtag atggtatttt gtcgattttg acaggaatcg cagcaaatca
    26101 ttctattgct tcaggaaaag cagtagtgat tgacgagctt gtcagcttaa aagaataaga
    26161 atagatttac ggaaattact tcccgtagtt tataaaatct tgccttattg ccgataggct
    26221 gtcttttgaa cactatttat tcggaaaaag gggctatgac atgctttttg tcatgccttc
    26281 tttttatgtg ggctgctttt ttagatagga gaaaataaaa ataactattt aaacccgaac
    26341 aaaaaggaat aatgcattaa aaatcattag attttacaaa aaaataagta aaccacgttg
    26401 tcagtttcag aaagtagtgt tagactgttt tcaatatttg aaacaacatt ttttattcaa
    26461 atatggcaaa ctgccttcac gtgcaaaaca gttgcaagaa aatgaattta cactattatt
    26521 agttttacat acctacttat atatcgtcat aatatggatg acagtttcta gccagtaccg
    26581 taaatgctgg actataagta aaagattggc tatttcaccc ttcttttctt atgataaagt
    26641 ggagggtgtt tttctttaac agaaacatag taggtttttg cacaaaagcg gttatttcgg
    26701 tattaataaa tgataaagga gcaaataaag tgaaagaatt agttgaacgt attcaaaaag
    26761 acggacgtat attaggcgag ggcgtattaa aagtcgacag cttcgtcact caccaagtag
    26821 accctgtatt gatggaacaa atgggaaaac ggtttgcaga agtatttaaa gaacaaggaa
    26881 tcacgaaagt tgtgaccatt gaagcttctg ggatcgcacc agctttattt gctgcaagag
    26941 aattagatgt tccaatgatc ttcgctagaa aagcaaaaag tttgacgatg gatgaagaat
    27001 tattgacagc ttctgtttat tcttttacga aacaagtgac aagtacgatt tctatttctc
    27061 gtaaattttt atcttcagaa gacaaagtat tgattatcga cgatttcttg gcaaacggac
    27121 aagcggcaaa aggattgatc gaattatgcc agcaagctgg agcgcaagta gaaggaatcg
    27181 ggatcgtcat cgaaaagtct ttccaagatg gacgtcagtt acttgaagaa atgggattga
    27241 gagtcgtttc tcttgcgcgt attgcttcct tgacgaatgg acaagttgag tttcttgagg
    27301 aggacgcata atcgtggaaa aagaaacaca aaatggaaaa gctgcggttt tagggctgca
    27361 gcatctgcta gcgatgtatg caggagcagt agcggtgcct cttttgatcg gtacaggact
    27421 aggctttaat caagaacaga tgacttattt gatttcaatt gatatcttta tgtgtggatt
    27481 agcgacatta ttgcaattga cagttaaccg atttttcgga attggcttgc ctgtcgtttt
    27541 aggatgtgcg atccaagcag tagctccact gatcttgatc ggaaccaatg aaggagtagg
    27601 agctatctac ggatctatta tcgcttctgg gatctttgtt gtgttagttt ctggtgtctt
    27661 ttctaaaatt aaaaaactat tccctcctat agtaacagga acggttatca ccgttattgg
    27721 tttgactttg attcctgtag caatcgagaa aatgggtgga ggtaatgcaa cagcagctga
    27781 ttttggtgac aaaacaaatc tgctacttgc atttgtgacg attctcctaa ttgtaggcgt
    27841 gcaaatgctg gcaaaagggt tcattcgttc aatcgcggta ttgattggtt tagtcggagg
    27901 aagcatctta gctgctttca tgggaatgat tgatctgagc gcgattggag atgcgccgtt
    27961 gttccatgtt ccacaaccct tttattttgg taaaccaact ttcgacgtat ggtcgatttt
    28021 actaatgatc atcatttcca tggtcagcat ggtagaatca acaggtgtat attttgcact
    28081 tggtgacatt actgggaaaa atgttggaga agaagaattg aaaaagggat atcgtgcaga
    28141 aggacttgcc gttatcttag gcggtatttt caatacattc ccttatactg gcttttctca
    28201 aaatgtcgga ttggttcaac tttcgggaat caaaacaaga aaacccatct atttctctgc
    28261 attcttcttg attgttttag gattattccc taaaatcggt gcgttagcac aaatcatccc
    28321 tgagccggta cttggtggag gaatgttagt catgttcggg atggtggcag tccaaggaat
    28381 ccgtatgctg ttggaagttg atttcagcaa tgataaaaat ctattgattg ttgctgtttc
    28441 tgttggattt gggctgggtt tcaatgttac gccgacatta ttcagtcaaa tgccgcaaac
    28501 attgcaaatg ttcactggaa atggcatcgt aatgagtagc attacggcta tcatattgaa
    28561 tttgatcttt aatggattga aaaaagatga agaaatattg gaagaagcct tcaagaagta
    28621 atagagcaaa agaaaggttg tgaaatcagc gttttgacct gattgttaga gaaacaaacg
    28681 gaaaaatagt tcctcgtatt ttgccgtttg tttgagctaa taccgagggt cagctgattg
    28741 aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc tttttttcat
    28801 ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat ttctttgaca
    28861 tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa acaatgacag
    28921 caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa gaagcatgtc
    28981 aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct catcgaaccc
    29041 cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa atcattattg
    29101 caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca actttgccgg
    29161 ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt ctttcgattg
    29221 tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg ggagcaatta
    29281 atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg gaggctaaat
    29341 tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat caacttggtt
    29401 aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt aggtcgtatg
    29461 atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc tgtgagcgat
    29521 tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga cacctttgcg
    29581 ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa cgtcagtgta
    29641 gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga cttgcttgcg
    29701 attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat cgtgattgct
    29761 ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg gatcggctat
    29821 ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta tgttttgaaa
    29881 agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg tgttttagaa
    29941 gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg tcaaggagat
    30001 ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca tgaaacgatt
    30061 gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc ccgtgtgatt
    30121 gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac aaaaacaggc
    30181 gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta ttctattgaa
    30241 gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg gccattagga
    30301 gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga acaactgctg
    30361 gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta tggaaaaaaa
    30421 caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga catccagcaa
    30481 acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa catgttagaa
    30541 cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata ccgtgcgtgg
    30601 ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat cccgcaagaa
    30661 gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt ggaaatcgaa
    30721 aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac attgggagaa
    30781 gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac tgcttatggc
    30841 tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt tacggaaatc
    30901 attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac acacggtgtc
    30961 catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga aatgaaacgg
    31021 aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat ctcaggagca
    31081 gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa acaattgggg
    31141 atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc tgaatatttc
    31201 tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat acgcggattg
    31261 caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa aggttcttct
    31321 gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct agccagagtg
    31381 gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga aagagatatt
    31441 tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt gaattacatg
    31501 ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa catgaagcgg
    31561 aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa attgatcgat
    31621 aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc gtacgcttgg
    31681 gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac atcggtatta
    31741 tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa cgttgacgtg
    31801 atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca attaaaatag
    31861 aatagcaaaa ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta
    31921 ttatattttc ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc
    31981 ctctttacat acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa
    32041 tgctggttat ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca
    32101 ttgaataaga tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg
    32161 attgaaagtt cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca
    32221 cagcgcttgg ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat
    32281 aaataaacgt ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt
    32341 aattctgata aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc
    32401 gaacccatta tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa
    32461 cgcttgttga tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa
    32521 tgccttaaat gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg
    32581 aaaaaacgaa caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca
    32641 tggagaagaa agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg
    32701 agaacttgat ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag
    32761 accagataag tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc
    32821 tgactcaaag aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag
    32881 caaaaaaagt acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt
    32941 tctcaaaacg tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct
    33001 actataagga tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac
    33061 aacttgcaaa accggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa
    33121 ttttgaccga attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg
    33181 gccggtcgaa tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat
    33241 gggatgaacg tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt
    33301 tgatcccagt ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc
    33361 gagtatgatg tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca
    33421 aggggaggca gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg
    33481 aataggaaaa tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat
    33541 tgaaacgatt tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat
    33601 caaaaaaatg gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt
    33661 gtgacgcaga tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac
    33721 ggcatgatga gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct
    33781 atggtgacta tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga
    33841 ttcgttttgc tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga
    33901 cagaagcagg acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca
    33961 aaactgtacc attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg
    34021 aaataatcta tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac
    34081 ttcaagaact aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca
    34141 gcgtggaaaa cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc
    34201 atccagaacg tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt
    34261 ccattttaaa aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag
    34321 ctgaagagat caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc
    34381 gaatgatcga agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt
    34441 ctgttatgtg gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc
    34501 ctactagtgg acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg
    34561 gtgatggact agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc
    34621 cttatgaagg agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg
    34681 ctcgtccaat tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga
    34741 aatatttact agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac
    34801 cgactgtagg cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg
    34861 caatgtgcgt aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg
    34921 gtaattcgat catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt
    34981 ttgcctcaga agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg
    35041 atccttttat ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg
    35101 attctctaat aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa
    35161 tggcttcaaa agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa
    35221 ctgggatgac accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg
    35281 ttaaatcagg agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag
    35341 tcactatcgg taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag
    35401 tagcaaatct gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga
    35461 aagaaccagc tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag
    35521 aacctggaca agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa
    35581 tctacgaaac ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg
    35641 ctgctgtgat gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg
    35701 cgcgctatct gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta
    35761 gaaatattat tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt
    35821 ctcctgataa accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg
    35881 cgtgtgaaac gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg
    35941 atggtcaagc aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata
    36001 aacatatcac tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga
    36061 cgtttgcaga ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg
    36121 tgatccgaaa ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca
    36181 tccaaaacgg attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac
    36241 tggcagaatc agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg
    36301 ctcaattatt tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga
    36361 cacgattcga agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag
    36421 cgataatcga aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa
    36481 agtgctggga ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg
    36541 gatcttcggt gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag
    36601 tcttcagcat cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa
    36661 ggggcatcga gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca
    36721 gttaacagga gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt
    36781 ggacaatatt cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa
    36841 tggtaacttg atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt
    36901 ccattctaac tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga
    36961 ttttttaaat aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt
    37021 aatgacagaa acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat
    37081 cggacaaatg aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat
    37141 tggggcacgt tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg
    37201 aatccagatt gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat
    37261 ctattttgcc agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa
    37321 tatgggacgg aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc
    37381 taattcttct ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat
    37441 cggattagta aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag
    37501 agagcaaggt gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt
    37561 gattatggtc gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt
    37621 gaaagaagca ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc
    37681 atgtttttac gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat
    37741 tgatgaaatc aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct
    37801 gatcgaatcg atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt
    37861 caacggagat tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa
    37921 agaaaaagta tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa
    37981 atgcatacgc aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca
    38041 agaagcatgt caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg
    38101 gttgttttga tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg
    38161 gcgtggggac gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg
    38221 actgcgtagc gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct
    38281 tagactactt agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg
    38341 tagcagatgg atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc
    38401 ctggtatgta ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa
    38461 aatctcaatt attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat
    38521 ctagcgggat tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc
    38581 agttttcttt atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg
    38641 agccaacgaa aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag
    38701 gagctgcaca tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat
    38761 ctatggctgc acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac
    38821 aaaaatatgg tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta
    38881 tggtcctggc agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg
    38941 gcgaaaaagt ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta
    39001 taggagaaga acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc
    39061 gctagccgat tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga
    39121 tcagcctgaa gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt
    39181 ttcacctaaa gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa
    39241 agaaaaaaag atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt
    39301 gctgaaaaat tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc
    39361 tggtttgcat ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat
    39421 tcattatatc gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat
    39481 tgaacaggag gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat
    39541 ctatccagaa gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca
    39601 ttgatcagtg tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa
    39661 ggagtagaaa tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa
    39721 acgctttcga tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca
    39781 cttcatccta aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag
    39841 gcaatggaaa cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc
    39901 aaagaaacga ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc
    39961 ggtggtccta gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt
    40021 gatccatctg actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt
    40081 tctttcagac aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg
    40141 atcgctcaat acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg
    40201 tatgaacgaa aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat
    40261 cagtccgctc ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa
    40321 ctttcttata ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac
    40381 gagccaacgg tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg
    40441 attttagaag cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc
    40501 atcgttttga atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta
    40561 gaaatcatca ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa
    40621 aacatccgat tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg
    40681 gtctcagtct taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa
    40741 gagtggaaag tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt
    40801 gcttggagag ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag
    40861 acagtaggaa tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa
    40921 caagcgcaag agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat
    40981 gattcggttg aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc
    41041 atcaaagacc aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact
    41101 gatgttcggc attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt
    41161 gcaggtggtc gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg
    41221 gtgtattgtg cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc
    41281 agtgagcgag atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt
    41341 atcgttggtc ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga
    41401 ttgaaaatct tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct
    41461 aaacaattaa tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac
    41521 gaaaaagcag cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc
    41581 ttagcagctg ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca
    41641 gaacaaatgt tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag
    41701 tttttggcag gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg
    41761 atggtcattt cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc
    41821 ggaatgggtg cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg
    41881 gaagaagttt taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt
    41941 gttttatatg cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg
    42001 cgtttcggtg atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa
    42061 gtaatcgatg atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac
    42121 aatgtaggcg ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt
    42181 ctaccagatt ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac
    42241 ggcaaactag ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc
    42301 aaagaagcac agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt
    42361 tatcgtacag atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc
    42421 tgcttccatt tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct
    42481 tttcataaag agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa
    42541 aaaaattcaa ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg
    42601 tatcaacaat gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga
    42661 tttttttgac catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc
    42721 tctagcaaaa acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt
    42781 tccaggtgtg atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt
    42841 gacccgtctg aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt
    42901 agaaccagag gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta
    42961 ttttggcgtc agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca
    43021 accattagta gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg
    43081 gattcatgtc aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta
    43141 ttcccgattg catgatatga cgatccaagc atggtcacca taccagtatg gattttttga
    43201 aggtgtattt atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc
    43261 agctaaatat caaacgacac caactggctt ggcttctgct tggattttac gccatccagc
    43321 caatatgcaa gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc
    43381 ttcagaaatt aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat
    43441 tttaccttaa agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa
    43501 cttgaaagct gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt
    43561 atcattgtac ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat
    43621 attcttttgg agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat
    43681 tataaccaat ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt
    43741 ttgctaattc gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag
    43801 aaaaaatcgt actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg
    43861 acatatctgg atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct
    43921 gatatttgtt ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa
    43981 ctgtcgaacg gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc
    44041 aatgaaccat ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag
    44101 tcaagacaaa taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt
    44161 gttcgatgta cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa
    44221 agtaaaagac agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa
    44281 tttgttcttt aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag
    44341 gtaattcgct gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc
    44401 ccttttctaa ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt
    44461 cttcaagaga atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca
    44521 cttgttgatt ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa
    44581 agtctgtact gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc
    44641 gattgaaaga ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac
    44701 cgttcaagaa atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt
    44761 tttgctgcaa atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt
    44821 taatcggttt aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga
    44881 aatcgtcgaa tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt
    44941 ttgaaaaagt caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct
    45001 gtaagtcgat aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga
    45061 ttggataata agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt
    45121 cgtcttcgat gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga
    45181 gggatcatca gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt
    45241 tgatcatttc cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata
    45301 ctgaaatctg aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca
    45361 gggatgccaa tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgcg
    45421 gtaatacgaa taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca
    45481 atcaatggct gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg
    45541 atcttatagt caagcatatg gccgtatctt atttttaaga tgactagata atcattgata
    45601 tggctaattt ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa
    45661 aagccggaaa gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa
    45721 gaaataatgg catccaaact gttataaata aaatggggag taatctgagc ttgcaataca
    45781 cgaacttcac tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt
    45841 agatcttccg ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc
    45901 agggttaaac gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga
    45961 ttgatcggtt cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa
    46021 atggtactaa gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc
    46081 gtattttttt tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac
    46141 agcagttgag tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct
    46201 tttgccaaga tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt
    46261 agcgatacat ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga
    46321 atatcattcc taagttcttc taggatattt tcctcattga actcatctac ggttacttgg
    46381 ccagaaatca agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca
    46441 tctgcttttt ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac
    46501 agtatgctca gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca
    46561 aaaatctgtt ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt
    46621 tgggatgtta gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg
    46681 taatgttttt cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca
    46741 gccgacatca ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat
    46801 catatcttct tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt
    46861 ttcttttatc gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc
    46921 gatttcggat aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg
    46981 aataaaatca ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc
    47041 atcttttttt agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt
    47101 ggtcggtgtg gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga
    47161 atttgtgaag tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg
    47221 gccaatgtgg gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat
    47281 gacagggatc cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa
    47341 aggagtaaaa atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg
    47401 ccgttcctga ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc
    47461 agtgataatt gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa
    47521 accaacgact agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta
    47581 agcgattaca acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg
    47641 gatttgttag tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg
    47701 aaaaaaatcc aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa
    47761 agtaaatccc ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc
    47821 aattgaaatg ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct
    47881 attttgaccg caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt
    47941 acggggtatg taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg
    48001 aacaacatgg tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa
    48061 gataaagtag aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca
    48121 cttgtgattg cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa
    48181 gcagatatca aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat
    48241 tacgcgactt ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac
    48301 ttaggattga aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac
    48361 gacaacaacg cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac
    48421 aacggtcagt tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc
    48481 tgggatggtt ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac
    48541 caaacattgg atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca
    48601 ttaaaaggcg tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat
    48661 gcgacagttg ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa
    48721 gacacacgta tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa
    48781 gaggttccgg tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat
    48841 ttagcaaata cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat
    48901 tattataaag aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct
    48961 aagtcagaga gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca
    49021 aaaaattttc tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa
    49081 tccatgcatt gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg
    49141 gcttgtatcc atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat
    49201 ttaaagattt gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac
    49261 taagtcctta tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag
    49321 gcgtgattga ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt
    49381 tgtctatcga cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg
    49441 aaatagctaa agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg
    49501 cattgaatga agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag
    49561 gtgtaacctc catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa
    49621 tcaccatttt acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag
    49681 agcatattat tcgcgggatg gtgggcagag atttagttga tcgttatcct gaaagaaaac
    49741 cggatattgg agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc
    49801 acgagcgaat cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa
    49861 tagctggact gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt
    49921 atggaagcaa gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg
    49981 ttccttcagc cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac
    50041 tgaatctgct gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc
    50101 aaaaaggagt attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga
    50161 tgcgtataaa aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac
    50221 aaaaagtcgt tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc
    50281 caacccgtgg gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg
    50341 cagcaaatgg caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt
    50401 gtgatcgaat ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg
    50461 cagatcagga agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag
    50521 gcaaaaaatc ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat
    50581 ctttttggtc gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg
    50641 atgacggatg gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc
    50701 catatattag tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg
    50761 tcggtagggt cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc
    50821 caaatgaacc cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca
    50881 tggcaaggtt tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga
    50941 ttgctgatgt ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt
    51001 ccagctgttt tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc
    51061 cttcacttct tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa
    51121 tggcgaacaa gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc
    51181 ttggtaaagg cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct
    51241 tatcaagggt atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt
    51301 ttgacaaata aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg
    51361 gctgaactat cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga
    51421 gtcatggcag ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa
    51481 gcaggtacat cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact
    51541 tccggcggca ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg
    51601 aacaatggca tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg
    51661 atcttgcttt tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag
    51721 aacgccggtt attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct
    51781 aaaagcgaac attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca
    51841 ttcgtataaa ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg
    51901 gcgctaaaaa aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca
    51961 ggaggataat gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg
    52021 tatcgtaaat agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa
    52081 aatgccagta gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt
    52141 agcggaaaat ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt
    52201 tatcaaaaaa atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga
    52261 ggaatattcc tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat
    52321 cgacatcgcg catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata
    52381 tttgcctgaa acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt
    52441 ggaaaatgcg ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac
    52501 aaaaatcaag acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc
    52561 gaaagcagct cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc
    52621 aaaatctgtc cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga
    52681 ggaatctcct ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc
    52741 tgcttcagaa ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca
    52801 acacaaagga tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat
    52861 ttcttatgct ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa
    52921 aaattcgatt tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga
    52981 aaaagttgtc ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt
    53041 tgtttggctg gaattattat cgcagataca agcttttgaa catcattatt cagaataagg
    53101 gactgtgata agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt
    53161 ctcgtaaatt cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg
    53221 gaaaaagtag agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga
    53281 gaaatcgaaa acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg
    53341 caactttttg gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt
    53401 ggggtactaa cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa
    53461 ttcgctgttc caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac
    53521 tggtatgagc aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta
    53581 tacgctagcg agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt
    53641 gaagaaatct tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac
    53701 aaaatcaaac ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt
    53761 gtgactggtg gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga
    53821 gaggggatag ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca
    53881 ggaacgcgtt tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa
    53941 gaatcgatcc ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct
    54001 acaggaaaag tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat
    54061 cggcttccaa ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa
    54121 gtccttccta tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg
    54181 gttttaggaa aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa
    54241 ctagctcaag cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg
    54301 gctaatgtat ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa
    54361 gaaaaaagcc gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca
    54421 attttaggtg gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta
    54481 gcagaaaaag tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat
    54541 ataaatggaa tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat
    54601 tcaaagatga aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta
    54661 aagaatataa cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata
    54721 cgatataaag aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa
    54781 aataaatgtt tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg
    54841 taagtttgat tagaaaatag aaaaaatgca gatgaaaaat aaaacgcttt ccttattgtg
    54901 attttattca ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat
    54961 ttgtgctttt agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa
    55021 atagtgatac agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg
    55081 agagaggaat gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct
    55141 agatgaagtc aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt
    55201 taacaaagat ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat
    55261 ggtatggtct cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg
    55321 cttttttgca ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga
    55381 taaagaagat gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt
    55441 acctttatct caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga
    55501 agaattgaaa gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg
    55561 tgttgcatta ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga
    55621 tggcggttca tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc
    55681 aaatgccgta ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca
    55741 aactgcagca aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt
    55801 cgatggaatg gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat
    55861 cagtggaaat ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact
    55921 atctggtgac gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa
    55981 agatccattg atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa
    56041 agaagaacaa gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga
    56101 caaagtacca aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca
    56161 atcaatcaaa gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa
    56221 aaaacaatga tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat
    56281 gtcgttgttt ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga
    56341 ttgcaggaaa agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc
    56401 gcaggattga gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc
    56461 tttggtttta ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat
    56521 cgaatgagcg gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt
    56581 catacaccag aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt
    56641 cgagtagtga ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga
    56701 agtaacgatc cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa
    56761 gtgccggatg aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca
    56821 gatgtatcta ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat
    56881 cttgcaaaag aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat
    56941 gtagaaacga tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca
    57001 caacgacaag cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt
    57061 tcattagaag ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa
    57121 gcagaaaatg tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg
    57181 gaattttaag aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACDXE010000010        57128 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.010, whole genome shotgun sequence.
ACCESSION   DACDXE010000010 DACDXE010000000
VERSION     DACDXE010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 57128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.010"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..257)
                     /locus_tag="IWM23_000580"
     CDS             complement(<1..257)
                     /locus_tag="IWM23_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883248.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAF"
     gene            complement(273..923)
                     /locus_tag="IWM23_000581"
     CDS             complement(273..923)
                     /locus_tag="IWM23_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883249.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(934..2106)
                     /locus_tag="IWM23_000582"
     CDS             complement(934..2106)
                     /locus_tag="IWM23_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAP9883250.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2125..2997)
                     /locus_tag="IWM23_000583"
     CDS             complement(2125..2997)
                     /locus_tag="IWM23_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9883251.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3019..3939)
                     /locus_tag="IWM23_000584"
     CDS             complement(3019..3939)
                     /locus_tag="IWM23_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883252.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3939..4208)
                     /locus_tag="IWM23_000585"
     CDS             complement(3939..4208)
                     /locus_tag="IWM23_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883253.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4205..4585)
                     /locus_tag="IWM23_000586"
     CDS             complement(4205..4585)
                     /locus_tag="IWM23_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HAP9883254.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5052..6515)
                     /locus_tag="IWM23_000587"
     CDS             complement(5052..6515)
                     /locus_tag="IWM23_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9883255.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6579..9596)
                     /locus_tag="IWM23_000588"
     CDS             complement(6579..9596)
                     /locus_tag="IWM23_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9883256.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9747..10586)
                     /locus_tag="IWM23_000589"
     CDS             complement(9747..10586)
                     /locus_tag="IWM23_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9883257.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10731..10976
                     /locus_tag="IWM23_000590"
     CDS             10731..10976
                     /locus_tag="IWM23_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883258.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11039..11461
                     /locus_tag="IWM23_000591"
     CDS             11039..11461
                     /locus_tag="IWM23_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883259.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11555..11872)
                     /locus_tag="IWM23_000592"
     CDS             complement(11555..11872)
                     /locus_tag="IWM23_000592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883260.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11899..12105)
                     /locus_tag="IWM23_000593"
     CDS             complement(11899..12105)
                     /locus_tag="IWM23_000593"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883261.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12086..12268)
                     /locus_tag="IWM23_000594"
     CDS             complement(12086..12268)
                     /locus_tag="IWM23_000594"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883262.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12282..12632)
                     /locus_tag="IWM23_000595"
     CDS             complement(12282..12632)
                     /locus_tag="IWM23_000595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883263.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12658..12960)
                     /locus_tag="IWM23_000596"
     CDS             complement(12658..12960)
                     /locus_tag="IWM23_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883264.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12963..13196)
                     /locus_tag="IWM23_000597"
     CDS             complement(12963..13196)
                     /locus_tag="IWM23_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883265.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14182..14504)
                     /locus_tag="IWM23_000598"
                     /pseudo
     CDS             complement(14182..14504)
                     /locus_tag="IWM23_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14627..15742)
                     /locus_tag="IWM23_000599"
     CDS             complement(14627..15742)
                     /locus_tag="IWM23_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883266.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15748..16146)
                     /locus_tag="IWM23_000600"
     CDS             complement(15748..16146)
                     /locus_tag="IWM23_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883267.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
     gene            complement(17175..17561)
                     /locus_tag="IWM23_000601"
     CDS             complement(17175..17561)
                     /locus_tag="IWM23_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9883268.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(17558..18079)
                     /locus_tag="IWM23_000602"
     CDS             complement(17558..18079)
                     /locus_tag="IWM23_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9883269.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWM23_000603"
     CDS             complement(18081..19115)
                     /gene="sppA"
                     /locus_tag="IWM23_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9883270.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(19148..20563)
                     /locus_tag="IWM23_000604"
     CDS             complement(19148..20563)
                     /locus_tag="IWM23_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HAP9883271.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(20532..22685)
                     /locus_tag="IWM23_000605"
     CDS             complement(20532..22685)
                     /locus_tag="IWM23_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HAP9883272.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGE
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            22943..23179
                     /locus_tag="IWM23_000606"
     CDS             22943..23179
                     /locus_tag="IWM23_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9883273.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            23198..23413
                     /locus_tag="IWM23_000607"
     CDS             23198..23413
                     /locus_tag="IWM23_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9883274.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(24116..24868)
                     /locus_tag="IWM23_000608"
     CDS             complement(24116..24868)
                     /locus_tag="IWM23_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9883275.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(24883..26130)
                     /locus_tag="IWM23_000609"
     CDS             complement(24883..26130)
                     /locus_tag="IWM23_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9883276.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(26206..26352)
                     /locus_tag="IWM23_000610"
     CDS             complement(26206..26352)
                     /locus_tag="IWM23_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HAP9883277.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
     gene            complement(26456..26767)
                     /locus_tag="IWM23_000611"
     CDS             complement(26456..26767)
                     /locus_tag="IWM23_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9883278.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(26769..26966)
                     /locus_tag="IWM23_000612"
     CDS             complement(26769..26966)
                     /locus_tag="IWM23_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAP9883279.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(27360..29087)
                     /locus_tag="IWM23_000613"
     CDS             complement(27360..29087)
                     /locus_tag="IWM23_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9883280.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(29080..30273)
                     /locus_tag="IWM23_000614"
     CDS             complement(29080..30273)
                     /locus_tag="IWM23_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9883281.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(30460..31104)
                     /locus_tag="IWM23_000615"
     CDS             complement(30460..31104)
                     /locus_tag="IWM23_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9883282.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(31198..31332)
                     /locus_tag="IWM23_000616"
     CDS             complement(31198..31332)
                     /locus_tag="IWM23_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAP9883283.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWM23_000617"
     CDS             complement(31334..33466)
                     /gene="feoB"
                     /locus_tag="IWM23_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAP9883284.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(33463..33936)
                     /locus_tag="IWM23_000618"
     CDS             complement(33463..33936)
                     /locus_tag="IWM23_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAP9883285.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            34337..34561
                     /gene="nrdH"
                     /locus_tag="IWM23_000619"
     CDS             34337..34561
                     /gene="nrdH"
                     /locus_tag="IWM23_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAP9883286.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            34720..36879
                     /gene="nrdE"
                     /locus_tag="IWM23_000620"
     CDS             34720..36879
                     /gene="nrdE"
                     /locus_tag="IWM23_000620"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAP9883287.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            36929..37894
                     /gene="nrdF"
                     /locus_tag="IWM23_000621"
     CDS             36929..37894
                     /gene="nrdF"
                     /locus_tag="IWM23_000621"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAP9883288.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(37983..39122)
                     /locus_tag="IWM23_000622"
     CDS             complement(37983..39122)
                     /locus_tag="IWM23_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9883289.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(39431..40144)
                     /locus_tag="IWM23_000623"
     CDS             complement(39431..40144)
                     /locus_tag="IWM23_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAP9883290.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWM23_000624"
     CDS             complement(40398..41099)
                     /gene="nagB"
                     /locus_tag="IWM23_000624"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAP9883291.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            41333..42865
                     /locus_tag="IWM23_000625"
     CDS             41333..42865
                     /locus_tag="IWM23_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9883292.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(43056..44258)
                     /locus_tag="IWM23_000626"
     CDS             complement(43056..44258)
                     /locus_tag="IWM23_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9883293.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(44340..45029)
                     /locus_tag="IWM23_000627"
     CDS             complement(44340..45029)
                     /locus_tag="IWM23_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9883294.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWM23_000628"
     CDS             complement(45135..45503)
                     /gene="rplL"
                     /locus_tag="IWM23_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAP9883295.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(45556..46059)
                     /locus_tag="IWM23_000629"
     CDS             complement(45556..46059)
                     /locus_tag="IWM23_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAP9883296.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWM23_000630"
     CDS             complement(46469..47158)
                     /gene="rplA"
                     /locus_tag="IWM23_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAP9883297.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWM23_000631"
     CDS             complement(47262..47684)
                     /gene="rplK"
                     /locus_tag="IWM23_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAP9883298.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWM23_000632"
     CDS             48027..48695
                     /gene="sdaAB"
                     /locus_tag="IWM23_000632"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAP9883299.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWM23_000633"
     CDS             48708..49580
                     /gene="sdaAA"
                     /locus_tag="IWM23_000633"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAP9883300.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(49671..50690)
                     /locus_tag="IWM23_000634"
     CDS             complement(49671..50690)
                     /locus_tag="IWM23_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAP9883301.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(50863..51750)
                     /locus_tag="IWM23_000635"
     CDS             complement(50863..51750)
                     /locus_tag="IWM23_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9883302.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWM23_000636"
     CDS             complement(51827..52372)
                     /gene="nusG"
                     /locus_tag="IWM23_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAP9883303.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWM23_000637"
     CDS             complement(52473..52643)
                     /gene="secE"
                     /locus_tag="IWM23_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAP9883304.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWM23_000638"
     CDS             complement(52667..52819)
                     /gene="rpmG"
                     /locus_tag="IWM23_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAP9883305.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            52981..53625
                     /locus_tag="IWM23_000639"
     CDS             52981..53625
                     /locus_tag="IWM23_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9883306.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(53681..54184)
                     /locus_tag="IWM23_000640"
     CDS             complement(53681..54184)
                     /locus_tag="IWM23_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9883307.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(54197..54243)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            54781..55746
                     /locus_tag="IWM23_000641"
     CDS             54781..55746
                     /locus_tag="IWM23_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9883308.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(55845..57017)
                     /locus_tag="IWM23_000642"
     CDS             complement(55845..57017)
                     /locus_tag="IWM23_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883309.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(57004..>57128)
                     /locus_tag="IWM23_000643"
     CDS             complement(57004..>57128)
                     /locus_tag="IWM23_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883310.1"
                     /translation="LYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
BASE COUNT        17012 a        11483 c         9386 g        19247 t
ORIGIN      
        1 aaaaaggcaa gtgcttcttt gtaggcattt tgactgtatt tttttgataa agcatgacaa
       61 atttcttgat aaaatagttg actgcctacc gaataccgat tattgtgtat ccgccacaaa
      121 acgaacacac tgatttgaat cgacgggcgt aaaaaagaaa aattctttcc ataagaaaaa
      181 gaacgtcctt cttgggtttc ttttgcttgt tctgttggta cttcttgtac ctctcttatt
      241 gattcttgtc tctccataac cttctccttt tcttattctt ctaaaagctg ttgattaaag
      301 ctacttccca aattttgcat aaacgtgatt ttctttgacg ctggatcaaa ggttacgtca
      361 aaaatattca tggcattatt ccaggaacta tcaccaattt tattgcgata cgacaccaca
      421 acgagccctt ggatagtcgc tttttgtgtg ctttttaaat aaacagaaca tttctctaat
      481 tcagaagaca ccccgtttga atcaccatag gaaacgcctt ctttgaaata ttgcttcatc
      541 agtttgtcag aaagatatgg tgcacaattg ttttttcgtt cttgaaaaga atcaggctca
      601 taattaaaca gaacctcgaa cgcttctgtc actgtttttt gaaaggtttg aatgtttcct
      661 ttttctgttg aatcattgtt tttcaattct tcatttgctt tttttaacgt cgtgacctcg
      721 ctttctactt gttgaagttg ttctttgagt gacgttacaa ggtcttcctg tcctttcttc
      781 tcttcttgga acttgtttat ctccaccgtc agtttctctt cctttgctgt tagctgtagc
      841 ttttcatgaa taagaaaaaa tgtactgcct acaccaaata agaacaatcc gcctacacaa
      901 acataaataa atcgtttctt catttgacac ctcttatttc tttatcggtc gagcaaaaca
      961 aatatcacca ccattaagca aatacccaaa ggataaattg acttgccctt cacctacacc
     1021 gttagtattc attccccctt gttcgattac tttcgtagaa agtccatgcc aatcttccgc
     1081 taaaacagct gtgtgtccat catttcccac accagaacct aagttcacaa tgataatatc
     1141 gccagctttt gcctcgctct caggtatttc ttgtaagtat tgcttacttc ctcttgcgtc
     1201 gctcgtcatt gagccggtaa accacccaac gttcgcaggc gttttatacc ctgctttcgt
     1261 taaaacaagc cacacaaagc tcgaacaatc ggcatagcca tttttatctg gattttcgac
     1321 actaccaaat tgtgcccgtt gcggttgtga ataatgaaaa taaccaatca gactttttgc
     1381 tacgtttaat acattactat cggaagaact gcttgttcct gttgtttcta gacaataatc
     1441 ggtcaagtct ttacttcctg cgtttgcccc attttctaat gtaattgaag cgacgggatc
     1501 actgtcattc tttcctagtt ttgcgtacca ttgattcgca aaagtaatac gattccccat
     1561 cattggtatc cctgcacgct caaatttcac ttgccactgt tctgttgctt cctctacgct
     1621 ttttgcttgt aatgcaactt gtgaagcttt ttctgttgtg tttagttcat gaatcaaaaa
     1681 ctcaacttgt acacctaaat ctgaccactc tttgtttcgc tctttggcaa aatttttcaa
     1741 agcagtcgtt cgccctgccg tccattgtgc aatcccatgc ccagataatg gatcgtttgg
     1801 tcgttcaatc gctgtgggac tcatactcga ctcttgttga aaattgccta acatacccgc
     1861 tagaccctct aatgtcgtat ttggaatatg tttttgtact tcctttgcaa tttgaagccc
     1921 gtttgtctcc atgccttggt tcgttaatga gatagtactt gcgacaattt cggttggact
     1981 acagtcgatt tcttcctctt cagaaccact actaatcaaa agtatcataa acagaaaacc
     2041 aaacatacat agtaatagtg tgggtagaca accgaaccca caccataaaa gaagtttttt
     2101 cttcatgttt gatccttcat tcgcttagtt cattttttgt gttgcttttc ttttgcgcca
     2161 aaaagcaaaa ccgcttcctg aagccaataa tccaccaagt gctgtcgcaa cgagagacac
     2221 ttcttcgcca gtcttaggca atactttcat ttttccatca gaccctttca ccgcaatatt
     2281 tccgcttgga agttgttcga ccttgtctga aatgggcgtc aagccatttc ctgattcttt
     2341 ataagcgaca ccatcaacga cggagacaat tttttctcct gaagctgttt caattggtgt
     2401 acttggtgtt ggaatatggg ttaattcaga cgcattattg gtttcaattt gaacattttg
     2461 tccacctgtt tgttggctgc caatacttcc atctggtttt actacgacgt cctgtactgg
     2521 aggcgtagtg ttcgtccccc cattattggt aggtggtgtc gttggaggag tcgtatttcc
     2581 ttcattgcct cctggattcg acggcttttc tggtgtctct gtattaccac catttcctgg
     2641 gttttcctca acgggtggaa cttcaggtgt tccttcccct ccgccagtat ctggattttc
     2701 tggtacttca ggcgtttctg tgcctccacc tgtatctgga ttttcttccg ttggtggaat
     2761 ctctggggtt tcaggagttt ctggtatttc gggaatttct ggggtttctt ctcctccatc
     2821 tgttggtggt aaaacaggtt cttcaggaac ttcaggtgtt tcttccgttg gtggaataat
     2881 ttctgtcggt ggtacacttg gatcacctgg tgacgtagta tctgcatacg cactggttcc
     2941 taccatacct agcaatacag ttgcactaaa taaacttcct aaaatcattt ttttcatgtg
     3001 tttttcctct tctcttattt attgatttat ttcaaaatca tctagttcgg gaagcgtgga
     3061 aactagataa tttttttcct gttcttccaa ttgaaaagac actttttttg tttcttgtgt
     3121 ctttttctcg gtttccaatg taatgacata cgcaatttgc caatgcttgc cttctttctt
     3181 catttcccag gataaaatac ttttgacttt ttccttttga ggactgattt gtttcgcaac
     3241 tttttccgtt agaaacgatt gaagcttttt cctataccgt tcttctgcca tttcacccga
     3301 ataataattc gtaataaaat aacgactaaa tgtatcaatt tgtggttgtt ttgtcgcaag
     3361 ttcttccgca aatgcaatgc gttcttgtaa ctggtgctcc tttttcgtta gttgttcaat
     3421 ctctctttgc gttgaaagtt gcataataaa aagaccacaa gatacaacga cgataaatcc
     3481 aaacaaaagc cattgaaaag acattttttt cttctgaaga gagccgttgc tttgtgtaaa
     3541 cgctgtttct acagaaagtg gtgttttctt ttcctttttg atttttgttg gtcgacgttc
     3601 gttcttttgg tctttcttta cttgataaaa cttcggtaat tcggaattta aggtttgaat
     3661 aaaggtttgt gcttcttcac ttaattgccc attttctgtt aaagattgtt caatcaatga
     3721 aatcaaatga aactcttcta ctggaaaatc atacgagcct tcaaatactg gaatttcatg
     3781 gttttctgtg tagatttcca aggtacacca gccaatttcc gctcgtgcca atagctcttc
     3841 attttctctt ttcaattgaa taaatgcctt atctaatgca atttttttat ctgttagtaa
     3901 agacatgtta tcaacttctg taaatcgaat catcatcatt agtcaaacac ttcttccacc
     3961 acttctaccg catacaccac ttgaataggg ttatttaaag aaaatttcac ccgatattct
     4021 tgattgttca aactgacgat atagctatcc tcatcttcag cataataggt atattcgttg
     4081 cctttgactt gaaaatattt ttggatcgct tgtctttgtt gccattcgcc aatctttgga
     4141 aaataggcga aatgatagcc aaataaaacg cctatcccta ataaaacaat tcctgttact
     4201 ataatcatct ggtttcctcc tgtacctgtt tatcttctat tgactctttt gtcggtaagc
     4261 aataaagaac cagtaacaca agagagccaa tcgttgggac aaaatagaaa aaaataaatg
     4321 cccaatgaaa acctgcgtct cgtaaacgtc gaacgctgag agaaagagac gggacaagaa
     4381 ctacgaaaca atacacaatc aatacgccta tcaaacaacc aattattgtt tcagaagcat
     4441 tcaacgctcc tgctagtcca atcgctaagc ctaggccaaa cccaatgagg gcattcatta
     4501 acgcaacgag ccaatactct tttcttgtgg ctttcccttt gtaattaaag ctattctgcc
     4561 aaaatttttt ataatttgtg atcatatctt tgtcctttct aatgtttatt tttgattcta
     4621 gtttcttctg ttttactaac aatgaaactt gccataaaaa aggtggccaa tacaccacca
     4681 gctagaaatc cacttacaaa taacaaaatt ttttcctcct ttctaaaaaa tttcgccata
     4741 aaaaggcaaa gtatgaacac gataaatacc atgtacatac tttgcctttt tatgcaatat
     4801 atgttaactc tccaagcaat tactgatttt taaattcttt ataaataaaa taaaaatcgt
     4861 ttgagtaaag aaataagtca atgaagaccc aaacgataaa aaatattcct aatactatat
     4921 ttaataaact acgttctaga tagatacgag atttcaaatt aaaaaacgaa aatgagagat
     4981 acaaatatat caaatattta aaaagtaacc acattttcta taccactcct tatcagaaac
     5041 agccttattc tttagaataa gactgtttct gggttgatta atttgtcttt tgttaaacta
     5101 ccatctaagg caagttctaa atgtaaatgt ggtcctgtac tatttcctgt tgaacccaca
     5161 taaccaataa tttgtccttg ttcaactgtt tgtcctacgt tcacgcaata ttcctgttgg
     5221 tgcgcataaa gagccacact tccgtcttca tgctcaattg tgacataatt tcctgacaaa
     5281 gtatgatacg aagcttctat gaccgttcct gccttgctcg catagattgg ttctccttga
     5341 ttggctgcca tgtctaatcc tctatgaaat tcttctcctc gccaaccgaa ggaactagaa
     5401 attgaataat tttttagagg taccatatag ccttctttgc ttagttgtgg accagctact
     5461 tctttatcat atttgattaa gtcataggtt tcgattaacc catttaattt tttatcgtag
     5521 ctagtgtctg ttgcgtatct tcctgttaaa aatttagtcg cttcctgata ggtttttgcc
     5581 ttacttttcc aaacaccatc ataaaaagag gtatttccaa ctaagccttc ttttaacagt
     5641 ttggcatagt cttttaagct ttcttcatag ctctcatatt gccgaaaagt tgcttgggtc
     5701 gtataaagtg tcccattgcc taaatcttct tgtgtcgcaa aagagacgcc ctttccgtta
     5761 tgtgtgcctt tgattccaaa tagattgtag tttggtgcct gagccaattg actttgtccg
     5821 cttgcggatt ctaaaatggc ttgcgcgatc ataacagaag cgtataaatc attttcttgt
     5881 ccaattttac gagcagattc accgatttta cggataaacg tttctacaga cgcgtctttt
     5941 tcaaaatgaa tagtcccttc gttatcttgt gtgctggaag ggactaattc ttgtatcggt
     6001 gcgcttggtg cttggtggta gttttttgtc tcttctgaac cgattgattg tgcctgcggt
     6061 acgtcattca taggtacagt cacactctgt tcaggtgcct tttgtgggcg ttccgttttt
     6121 gttggcggtt ccgcttctgt ttcggtactt ggtttttgag gagtctgtga gctttcatca
     6181 gaaggttgct catttggttc ttctgacggt tcctcctcag gttgttgatt gtcttctggt
     6241 ttgtctggtt gattcggtgt ttctggttgt tcttcagaac ctgttggtgg agcgttcatt
     6301 tctggattat ctggtgtatc tattgtatct tcagtcggtg tttctggttg attttcttct
     6361 ccagtggaat ttgttgtcgt ttcttcagtg atcgttgcag attgcgaatt tgtcagttga
     6421 ttgttgcctt gttcattagc caaaaccgct ggcgtatacc caaatacttg acctactaat
     6481 aacaaagcag ataaacattt actcgttttt ttcacaagat tgcctccatt cttcaatcaa
     6541 tcatctagag acagctgatt gctgtctcta gtcatacttt aattgctttg tttcatttta
     6601 cgacgaacca gaacagcccc cacaccaaca aaaagaaaca gtcccaatgc aattaacaca
     6661 tcgttcactg tttctcctgt ttttggtaag ctttgttttg gtgtgctcgt cggcgtttgt
     6721 ttgacagggg ttgtcggcgt ttctttttta atgccttttg ctgtcaatgt ttgtttttca
     6781 ttcttcaaat caaagtgacg tgcttcttcc tctttatggt ctttatcctt atagacaata
     6841 tgagttgcga ccaatttctc cccgtctttt aacgtgcctg ctttgtagtc aaaggatact
     6901 ttaaactctt cagacgcttt agaaacttta cgtgcagttt ctaccgtacc aacgactgta
     6961 tcttttccat ttttatcaat cttatggaat tgagtcaccc agtatttggt ttcccctacc
     7021 gtagattctg gaaattcttg cgatacatga tcgatcaact cttggtcttt gtctggatca
     7081 aatgtttggt cgccattcac tgtaataaag cgtgtttcga tttttggttc tttcgcagtc
     7141 actgtttgat ctttattggt taaatcaaaa tgacgagcga attcttcttc ctcttctttg
     7201 tcgttataga caatgtgcgt tgcgacaatc gtgtcaccgt ctgaaaccat gtctgctttg
     7261 tagttgaacg tcacatcaaa tgtttctttt tcatcggtca cttttcgtgt ttcttttacc
     7321 gtatctaata cagtgacttt tccatcggca gtaattttat ggaattgcgt cacccaatat
     7381 ttctctgtcc caaccgcaga tttagggaat acttgggaca ctttatccac taattcttgg
     7441 tcttttttcg ggttgaaatc ttttccacct gtcgttgtca caaacaaagt agaaatttct
     7501 ggttggtctt cttctggaac cacgatttct tcgtttttca cagtaattgg ttgtacttct
     7561 ccgtctttga ccgttactaa aatcgtttct gtttgtcctg acgcttgccc ataatgctca
     7621 gggaacacaa tttccgtaaa tttatacgta ccgtctggta attttagttt ttctggttct
     7681 tcttttgttt catacacaaa ggacacattt ttattatgtg cagtcatacc ttcaatcagg
     7741 aatttcgctc ctggtaattg tagatgattg attgaatcga ctttattgaa gtctacttca
     7801 cccgttttta acaagttgtc aatcacatct aattcaaacg taagcacagg cgtcgtgtta
     7861 tcgccatagg ccacagagaa cgtggtttgt tcttcagaag gttgacggta gttgtctggc
     7921 acattttttt caatcaattg gtatttgttt tgatcgccat tgtagaattt tttgtttgcc
     7981 ttccagtgtc cgtctttgtc agtttttact gaatccaaga cttctccttt tttcacaagg
     8041 actgtttctt tatctggcgc agtaatatct gttttggcga taatatcaaa ttcgatattt
     8101 tctgcaggaa cggtatcata aacaggtttg ccttgattcc aggattcttc cgcattatag
     8161 acttctttga ctttctttcc ttctggaatg aactgttgta agtcattttt tgcagttaac
     8221 gtcacttctt tgtttggttc aatcgtaatc gtatgagttt gtttgtctag aatataccca
     8281 gctggtgctt tgacttcttt gacatccaat ttattttgaa catattttcc gtctttttca
     8341 tagaatgcag ggatttcttt taaggcgatt tcccctttgg catccgttgt atattgtttt
     8401 gtttctcccg tatcatgacg tttgatttca tacgtcacat ttggaacttt tttccctgtg
     8461 acagcgtcta gtttggttag tttgaagtcg ccatttttta gaactttaat agttagtcca
     8521 gctctattag gatctggcac ataaagcttt cctagatttt gagcaacagg atctacatag
     8581 gcaaaacttg aacctacccc tctagaattt ggatccagtg aagaaacaga aattcgtccg
     8641 tcatttgagt tttgatttgc ttttataaac aaactatttc ctgattttct tacttgaacg
     8701 ttagctgtgt tttcaacaac cgacccatcc cattgagaaa acacaccttt tgtatcctta
     8761 acttctactt cttcccctac tttaaccgtc actgtttggc tatcaaaaga aggatcttca
     8821 agtaaattgt ttatttttct atctacagtc tgtttgaaag cttcataatt agattgtcca
     8881 atagttccat aattaacaaa tgaaccacct aaaacttccc aaatatatgt ttgtgctaat
     8941 acatagttat ccattgaacc actagaatag tagccaaaat aaccaattaa agaaatcttt
     9001 tttctaatag cacttgtcgt aattaaattt tcgagtggtg tcacatcata ttgaataccc
     9061 gcactcgtga ttgcgccttc ttgaatgcaa aaaacatctt ctccgtcaat ttgccagcga
     9121 accatgtaat catgagtata cattgttcct tcaggagtaa ttcgagtcat attcacatct
     9181 ttacgaccat tttcagtcaa tgttagattg gtagcacgtg tctgtgcatt cttttccaag
     9241 attgcttgcc attcttcaga ttcttgcgcc aatcgttctt gttcagaaaa agaagtcgct
     9301 ttttgaatat cttcaatggt tataaagtat ttctcataac cttcagggac ctcttgagtc
     9361 attacattgg gcggagaaac atcttctgtc gtgtcctgcg tagttgtctc ttctgttgtt
     9421 tcacttgtgg tttcagtact gatcgcttct tcactacttt ttgattcttc agtcttcatt
     9481 acttcttcag tagaatttga ttcatttatc gtttctgcaa atgccgttac agatcctaac
     9541 atgggattga gaaatgtaac gaaaattgcc aaccaagttg ccattctttt aaacatattt
     9601 ttttcgttcc tttcatttta tttttcagtc ttctaaagta atttcttatg gacttcgaca
     9661 ccgaaacatt tttatcctcc tcaaaacaaa aaaagactag aacttataat tctagtcttc
     9721 atcaaccact tacttttctt ttatttttag tagaaatcta cagtgtgttt ttgtgacccg
     9781 tcactgtaaa taataacatc caccataaaa cctttgttgt accactgcga atttggatcg
     9841 ctaacctctg cattagccca atcataggct tcatctacag tgttaaatac ctttccacta
     9901 ttaccaatct ctccaactac aattgttggc tcttcaggtg tcgttgtgcc accatttccg
     9961 ccttgatcgc ctccaccagg attgcttggt tcttcaggcg ttgttgttcc tccacctgtg
    10021 cttgggttag aaggagttgt ttctcctcca gtatttccac cattacctgg ttgtgtaggt
    10081 gtttgagtag tgccaccatt gttttctggt gtacttggcg ttgttgtacc accagttgtt
    10141 cctgtttgat ctccagctgg tgttgttggt gtcgttgtat ttggtgtcgt ttgtgtattt
    10201 gaagtcgttc cttgtcctac tggttgattc ggatcaactt tgtctttctc ttgaatcact
    10261 gtttgagcca cagtttcacc tgcgctgttt tgtacacttg ctacattgcc attaaatact
    10321 aatttatttc ctgcatagat taaatccaca tttgtaatct tattcatgtc cgctaatttt
    10381 tggacagtaa tatttgttgc ttgactgatt gcgcttaggg tatctcccca gacaattgtg
    10441 tattcattcc ctttaatttc tgctttgatt tgttccgccg aacgaggtgt ccaaactttg
    10501 ttttctgctg cttcagcatt cgttcccaca gcaagactaa ctcctccgac tacgattcct
    10561 agtgccaata ctttcaacat tttcattgtt tttcgcctcc gatttgaata aatttattta
    10621 caaaaacata gtaccaaact tatgtctatc agtcgatata ttttttgtag atttttttat
    10681 tttttgatat actaactttg gtactaaaat aactaaaagg aatgtcacac atgcaactta
    10741 ataatttaaa tgaaatacat gattgggctc aaaataattt acttaatcgt aaacaagctg
    10801 ccaaaatcac tggacaaaac tatgatgctt tttctagctc tttacgtaga aataatatca
    10861 aaccatttct agaaataggc gacgaacctg gtccttccat cattcgctta tatctaagag
    10921 aagatattga aaaatacgca aaacagctcg aagaaaaaag acagaaacgt caatgaggcg
    10981 ttttctgtct tggatttaca agtaaaacaa taataaacac ttttacaacg gaggttctat
    11041 gaataagaaa tacattgttt cagttggttc aatcgtactg attgtactcg ctattattgc
    11101 tttttctgta tccaacaaaa cagaaacaca gccacaaaat acggttcaat cgactactac
    11161 aagtcaatct agcgatcatt ccgtctatcc ttcactaaat agtttaaaag aaacgcatgg
    11221 acaagattta attgatcttg gttcttctca aattactact gtcttatttg aaaagaaaaa
    11281 tattgcgttt attactccta aagaaggaac tgaccttgaa ttttctatca aagtaacaaa
    11341 taccaaagaa aatttattct ccattcctga cattcaatat acccctaaca agttaaagat
    11401 tggggatacg tttggtttgg ctaaagtaaa tgaacaatac tttgcttact cactgaaata
    11461 agtttctaat agcaagagga aaaaccctct tgttattttt atcacataat cattttataa
    11521 aaacgcttcg aaaatatcaa catttttgaa gaaactattt ttctaatttc tcaatttctt
    11581 ccaagggatt ttcgctaatg acccatacag gcacagattg ctctcgaggc tccccttctt
    11641 ctaaccacca acctgcataa tgaacttctt cctcctcagc attaccatga taccaaccaa
    11701 caaaggattc atctacaacg aatgaaattt cttcttctgg atcaaataat tcatttttcc
    11761 atgcttcaat tgcttgttct ttggtgtact tttgtttgtt aaatacacca caaatttcgt
    11821 catctacata aaaaatttca aaatcaaaat tactcgttcc ttttactggc atcttaactt
    11881 ttcctccttt gactcacttc aatagatata ccactcatgt tcattccatt gtttactagt
    11941 taattggagt tttatcttag ggctttgtgc actctttcca aaaacttgag cgtcgttttt
    12001 ttcttctaac caaaaaaggt aatttcttac attcggtact aaatgttttt cctgcatttc
    12061 ttcatgaaaa acatataatt ctgcttcatt tttcaaccaa ttcattattc attccctcat
    12121 tcacttcttc ataccgccat tcagatagtg agatttcttt ttgtttctcc acttgtccta
    12181 taaaaatcgc tgtattcaac aagctgatca cacttaataa taaagcaatg aaactaatgg
    12241 ttacacttac tctttcacat tttgtcattt tgtttccttc tttactcgtt tgttatttct
    12301 tgtaataggt ctaataactc cttcaccgaa atttgcaaat ggttcgcaaa aatttcaata
    12361 aaagcactcg cacacattac cagaccttca actgatatat cactgcgaca ataaaattct
    12421 ggagaaacac tgatttctga ttctttctta caattgaacg aaaaaactat ttttgaatca
    12481 aattccggta attcttccac aactatgttt tgggattcta ccaagaaagg gagtgcctgt
    12541 tctaatgctt cttctggtgt ttgagcagat agaataattg cttcttgttc ttttatttca
    12601 ttgttctcaa atactttata actttttttc attgttagtt cctctttcat tcctctctca
    12661 attagctaag ccgttactgg tacgccattc tctaaattta gtatcgctaa tccttaatgc
    12721 tcgtcgaata tcaatcattc gataacctat tgcacgaagt tctttatatc ttttagcagt
    12781 tagcgtctct aacgtcattt caggcaatgg gttcgaatca aaaatagaat tcgaaccgat
    12841 tatttgggcc aattgttgta cttgtaacag ctcttctgaa cttaattcag tgattgctcg
    12901 attgcctatc attgcataca ttttctgtcg aatatacttc ttttcttgtt tagacaacat
    12961 ttctatccac tcctattttc attatcattt gttacctcca cttcagtaaa tccacaaata
    13021 aatgaattca tattccaagt accattccct tcaactacaa cttttctcct gtcctctttc
    13081 ggatattcaa gaatcagacc gtttaccaat atgtttttca ctactactaa tttgtttgta
    13141 taccgtggaa ttgctgttcc gatatacttc actttatttc ctacgctaat tgtcatttcc
    13201 tcactcctat tcttttttat ccaaaacttt ttcctgtcct attcctgcca acgaatatac
    13261 ttcactgcat gcttaaacaa aacaaaatgt tgccctgttt tatttccgtc ttttacttgt
    13321 tccacataca gttcatattt ggtcacacct tttaaaacgc cagatacttt ttttcatcca
    13381 ttgtcacaat tagttttttc atcaatcgat tgatactgca ctgctcattt ttcttgttca
    13441 tatagtattc ctttcttttt caaaactagc ctcttttgtt tactagtttt catcaatcat
    13501 caacgattta acagattttt tctttcgcag ggtttctagc gtcagcaacc ccaaactcta
    13561 aaaaagtgcc tttgctccct tgaaaaacga gcggacgatt gtactcgtca tcatatgttt
    13621 ttcatactac ctgatccacg caaataacct ttttttgttc tgtgccacac ccatccatac
    13681 gtatcgcttg agattcacca tactttttta ggtggtttat ccttcctaag tccctgttag
    13741 acacgctgat taacttagaa agtcctgttt gtatccaatt ttcaaagatc tcccaaaaga
    13801 aataagtagg tttacgtgta tcgcttctgt tcaaagtacc tgtttgtact tgagtatttc
    13861 tactcccctc gttcaaggga ttccttccac cagctagctt ttcctttcat catcaaatgt
    13921 tcctgaaaag catttacgtt tcgtgtccgt gtgcttactt attctttttt cgatagcgat
    13981 tatccgctcg ctactcgcta ttttatactt tgtttgttag ctcaaataaa aactaacaaa
    14041 caaaaaaggc tagtttcttt ccgttttatt tttttaactg tcgataaata gttgttagtt
    14101 taattccaag ttctaaaacc aatacgccca acaaaaccac aggaaaaaca attggggcaa
    14161 acaagtaaaa tcggatagtt ttcatgtctt ccctctttct ttttggtgtc gctgtggtcg
    14221 ttcttgccgt tcaatacttc gatacatttt ttattcatta tttgctcgtt ttgttttcct
    14281 ctggactgct tttcttgctt tttgaatact ttatacgctt ttcgtgcctg aattaatagg
    14341 tcttgtcggc gtttcattcg tgagaaaaca atatttgaaa ccatcaaatc ctggttcgaa
    14401 caaccacgat tcgcaataat tttcccatca aaaaaaatag ttttcacttg agaaaaaaac
    14461 agttggaaat cttgttttgt tgctaatttt tttgtcaaaa tcactgtgct tttccctgtt
    14521 gaatctacaa tgattcttcg aacaatccga ttcgataact tccaacgttg ccgattaaac
    14581 aatgttactt cacaacaaat atgacgtaag tgattagggg tcattttcat gtgtccaatt
    14641 cctccaacca attatgcatc gaaattttgg gcaattgtga cagcccataa atctgcgttt
    14701 ccaggtcttc ataactacaa gaacgcttgg cttgaagtaa tgaatattct gcttgatctg
    14761 atagttttgt tgccattgga tctagattca attctcttac acaacattcc caaaaattca
    14821 atagcgctct aaaataaaat ccttttcgat tctgaattgc ttttccttca tgacgagcaa
    14881 ttttttcatt ttgaatacac cgttgatacg ttttgcttaa atcttctgca aatgtttccc
    14941 ctaaaattcg atactcgctt ccatcagata aaccatgtgt ttgattatac tttcgttgcc
    15001 gcttttctat aattcgttta ctgtcgtata tcatgtttag ctgctctttc gcttcacgcc
    15061 attcagctga actgcgaaca atccatgtca aaacatcttt cggaacaaac gtactttcgc
    15121 tgtaatgagc aataaactct ttttgccaat catcagccat ttcaacagga ctagtcgtat
    15181 cgatttttgt atctagattt tctgtatcta gattgttaga agaattatta aataattgtg
    15241 cagaattttc acatggattt ttttcgtcaa aatcatcgaa aatcgctaaa ttatcaacag
    15301 attcctcctc ttcttttcct tgtgcagaat ctacactggt tgagtcaaca ggacctaatg
    15361 aatacacatc attttctgtc gtatctaatt ctcctagata caaccgagct ttgtttcttt
    15421 ttcctgtttg ttgatcaaat cccatatcct ctatttctag tagctcatat tctttcaatt
    15481 cttttttgat tgtattaaca gaacgtctgc aacaattcaa tgcattggct aattcttgtt
    15541 ccgtaaattc aaaataaaca aaaccttgac tatctacttg atttcgttga atggctaaat
    15601 ctaagcgtga ccgaaaaacc atatacgcga tttttgcttt atcagacaat gagcgatatt
    15661 tttcacttgt aataaatacc tttggaaatt gatagtataa ttcattgtat ttacgagttg
    15721 cggtaaatct ttttgttgcc atcgttatta cacctcatct tgtccatata ttaagaaaga
    15781 attttggtgt tggtagacac gaacatcttc cattgtagca atcaattctt ttagataaat
    15841 tcgaccgaat tcaattgtta gtaagtcgta atcttctaat tcatttttga tttcttcaat
    15901 ttcttcttca gacacattga gtaaaatggc taattcttga ttggtataag aaatatagac
    15961 atgtttcttt tcatcaatcc gtttttcaat taaggattct tctaagtact cattcagtaa
    16021 gctataggcg tattttgctt gtgcagtcat cgcataatat tttgacgatt ttataaagaa
    16081 tagttttggc atatgaaaag ttgcttgtgt cagcacatct gttgcgcgaa atttgttttt
    16141 taccatattt tttcctccta gaaaaacacg caaaaaaagc tctgtataaa tcgaattaca
    16201 cagagtttct tcttgttgat atttaatttc taaaatacgt ttcgggtaaa aactaaacac
    16261 ttactaatat ttagattgta gaatcttcgc cttcatttat tcattttaaa gagaaaaaga
    16321 tcaagtgaaa tgtgcccaaa agtttgattc tattaatttt taaacaccat tcataatata
    16381 tttcctataa tgtaaaaagc gtcgatactt attaaagcat cgacgttatt tttatagtaa
    16441 atttatcaaa atttttgcca acattttttc cgattttttt ccctatttct atctttcctt
    16501 atctttctta gttaatatta tttgatgtta atatatcact actaatatta aaaaacattg
    16561 atttatcata ggataaagag tgatagttta atacttaaag agacatatta attttattta
    16621 aaaattgaca ttaatgactc ttaatcagcg ggtcgcgggt tcgagcccct cacggcccat
    16681 tgggtgccaa acccacggaa caggtaaatg ttggtgcgaa agcatttcga aaacatttac
    16741 gctgttcttt ttttattttt caaaataact agggcttttt atttttaact caaagtccac
    16801 tgtagccctt tggactttga gttaaaaaag aagcataatt atgaacagta aagtttctaa
    16861 tataacaata attcgagaag ctttgcaaaa tcatgctgcc aatgaactct ctaaacatat
    16921 aggattaaat ctaagtacta taaaaggtta aaaagtgaag aatatttaat agaaaaacta
    16981 aatttacttg ctgctttccc cttttccatt aatacaacaa tcagagagtg tctttttttc
    17041 gaaaaaatta gaataaataa aagacacgta gtttttttat ctacatgtct tccatcgact
    17101 acaagttatc atttctcaaa acgtatttag cttttaattg attaatcgtt tcacgttgtc
    17161 tacacattgc ctaattaaga ttttcttatt agaagcatca atcccgcaat aatacctaaa
    17221 actccaggaa ttacccacaa taatgttaaa agaaaaaatg gaataataga taaaatgagt
    17281 aaactaatac catagaccac tgttttttga ttttttctta ctaaaaaacc tattatgaaa
    17341 atcattatcg aaaaaactag ggtaattcct ccaaataaaa caacattccc gcttagattg
    17401 actaacatat ctatatcctt actggttagt tctgtagtat ttaaagtagt tgaatcaata
    17461 gaagatagaa acataccacc catcaatgct aaaagactac ataaaatccc tgttattcca
    17521 ctaattaaaa tcaaaataaa ctctgttttt ctcttattca tttaattctc tccagattaa
    17581 gtgttttaga attagaaatt ttttgaccat agttaatagc ttctaatgaa cgaataacaa
    17641 ccactgacgt gttagaaaaa taatccccaa tatgttgttt cttgggcgtg aaagcagcca
    17701 ccaaataccc aatttgaaat acaaaatttt ttaatataaa cctacatata cactcacgaa
    17761 taaagatagt ttgccaactt agctcttttt cctcaaagct cactactctt atcccaaaaa
    17821 tcattttccc aattgtttgt cctgaattca gcttagtcaa aagtacaaaa taggttaaat
    17881 aaatagctaa tgacgctata ccataaaaag taataatgga tttgtcagat tccagtccca
    17941 tgattttgta aaagagacct atagtagcat gggtgatgat gctaatacaa attaaatcaa
    18001 taagaaaagc aaaaaatcgt aaccaaaatc ccgcaaatac ataattaggc aaagtttcaa
    18061 tgccactctt cttattcatt ttatagccct ccatagtaat acattggttt tggagtatca
    18121 gaaaatccaa gtttttcaaa aactgaaatt acacgatctg atgtagttgc ctttagccct
    18181 tgccattcag caatcttatt acctaaccaa gaagaagcaa agccagttgt gtcattttta
    18241 tattctataa ccgtcgcatc agttaatttc ttctcttttt ttagggaatc taatgcatct
    18301 tcagagtaac caatttgatc caccaaccca ttttctactg cttggttacc atcataaatt
    18361 cttccatccg caagttcttt cactgcatta gtatccatat ttcttccttt tgctacaaca
    18421 ttgacaaaac gatcataagc actcatcaca aattcctgta taactttatt ctcttcctct
    18481 gaaggtttgt gttgaggtga catcatatct tttaatgcac cacttttata cgtcgcatca
    18541 gttacgccaa gtttttctaa taagccagaa taatttaaac cagaaataat gacaccaatt
    18601 gatccagttg ttgtttcttc cgtagcaaat attttatctg aaccagcaga aatgtagtac
    18661 cctccacttg ccgctgtatt tttaaatgca gtatagatag gaatatctag ctttttaatt
    18721 tttgccattt ctttagcaat ctcagcactt tcatatattc ctcccccagg tgaatttact
    18781 tctaataaca ctcccttaac cgctttgtct tcttgaattt tctttagctg tgttaaaaaa
    18841 ttctgatggt tatattgttc cctactaaaa agattgcttt cacctgtatc tgcaataact
    18901 ccattgacgg atagtttgac aatcttttta tttgatgcgc cttcttctaa cgtttcttct
    18961 gtgatttcgt tagatccgta aaaaatctta tttattcctg ttagggttgt atttgatgct
    19021 tcctctttat cttttcctgg aattagcgcg acgataatgg atagtaacaa taatccgcac
    19081 gctactccca cagcaatcca tcttctttta ttcatataaa ctcctcctat attcttagta
    19141 aaaataatta ttttttattt agaaacacat cgacaatttg ttgccaatat gttttttctc
    19201 ctacaattaa agacaactct ccagttaatc cataacgaaa cggaaacgat tgagttgttt
    19261 ttaacgttcc tttcactgca taaaaaacac ctttctcagt ggcagtagaa ttttctgaaa
    19321 tttcatctaa agttccgtcg atcgtaatcg gagccccacc tttttgatcc aatttaaaat
    19381 gaaccttcat cccttcttta atataggtac tttcattcgc aggtaaaagt gctgtaaatt
    19441 tcatttttga tttttcgtgg atcggataaa tttctgcaag cactgttcct ttggctagtt
    19501 ctgcttgtcc ctttgtttct tcatttaaat gaaccgttcc agatacagga gcttttagta
    19561 ttccttttga aagttgatcg tccaaacttt tgataactga ctggttcttc tcttgttttt
    19621 catttagctc tgtaactgtt tgttttacag ttgttattgc ttgttctagc tcttgctttc
    19681 ctttttccgt ttgactaatt ctgtcattct cagaagtagt tggtttaact aatttagctt
    19741 tttctgactg atactgttct acttcttttt ttagttgctc aatttgttca tttatgcttg
    19801 ctgaaattgt cagtttcact tgattttttt gatcatcaga aacattattt agttgttctt
    19861 gccaattctc atagtttgcc atcatttctt ttgaaaaatc ttttaatgat tgattattac
    19921 tccaagctac ctgtacttgt tgccattctt gtaatttact atctgaactc tcaatttgtt
    19981 tttctagttg tctttttgtc ttttcgtata cttctaattg cttttgatca tttaattcac
    20041 tttgcttcaa agcgtagcgg agtgattctt tttctgacaa catgctcttt aattgattac
    20101 tgtagccaaa actgtcatcc gttgaaaaca aattttttcc tttacttata ctttcaataa
    20161 atagctgagc catctttatt tgttgagtaa tacgttggtt ttcttgttct atctgagcct
    20221 tttcattctc tattaaagaa caatccaatc taaccaacac atcgtcctta tgaacaaatt
    20281 gattttcttt taggaagttt tccattattt tagcttctaa tggcacttgt aacttctcta
    20341 ttttttcggg gttcaattga gccgttgtcc gaataacaac ctctttttta gcaaaggtta
    20401 aaaacaatcc aataatacat aaaaaaagaa ttacagggta taacacccaa cggtagaagc
    20461 tatgatgttg tttagtatag atactagaat gttcgaacca attattattc tgcataaatt
    20521 gtttccctct cctagatttc ccataatttt tgatagatac catcggcaaa acgaagttca
    20581 tcgtgcgttc ctttttctac caaatgccct tcatggagaa ctaaaatttg atcacatgct
    20641 tttgctattg gtaaatgatg cgcaataaaa ataatcgttt tttcttttaa attgaggaga
    20701 taattcataa ttgcatgttc taacaaggta tctaatccac tagttgcttc atctaaaatt
    20761 aatatgtccg gattttttaa taaagcccgt gctaaggcta atcgttgtcg ttgctctcct
    20821 gaaagattat ttccaccttc ttccaacaat gtctcgtaac gtaatggctg ttgattaata
    20881 aattcattta gttggactat ctcacaaact tctattattt gttcgaaact tggtgggcta
    20941 tctaaaccga atataagatt ctcaatgatt gttccactaa agaaaaatga ctcttgaggc
    21001 aaatatgtca ctcgatcgcg taatatctga aaaggaatgt ctaagtgatt aatgtttcca
    21061 taatggatcg atccttctga tgcttcataa aaacgaacaa ataactttgc aagagttgat
    21121 tttccagaac cactaactcc aactatagcc gttttactgt gcgaaggaat tgtacatgaa
    21181 atttcgttca acgttggggc cttcatgcta tacgagaatg aaacgttttt tacagtgata
    21241 tcttgttgaa ataattgttc agaaagcata tattcaaaag actggttcga tttttctgaa
    21301 tcaatcgcga aaatttcatt taaacgatta tttgcaactt gagccgtttg cattttcact
    21361 tgtaaattaa taatgttttg taaaggatct gtcaaaaata ctagtaaagc attgtatgtg
    21421 ataagttgac ctagactaat tctattatcc ataacaaaat aagaacctag ccataaaatt
    21481 aatgcactgc taattaattg aatcaaatgt ttaattcctt gttgaatatt atcaagcgta
    21541 accgtacgaa atgatttttt cattaattta acaaattcat ggtctatacg gttatatact
    21601 ttctcttcac cgctatatgc tttaattgtt tctattcctt taagactttc tatgatacta
    21661 gaatttacag ctgcaccggc tgccatttct tccctgttcg atttttcata gcttttcacg
    21721 aaagcgaata ttgcaactac ataaaaaggc aaagagcata gagtgattaa aaacaacgta
    21781 ctattctgaa tagctaacgt aaccccaaca agcaacacca taccaatatc taaaaatatt
    21841 gataacgtag cacttgctaa agcatcaata atcttactgg catcaagaaa acgagaaata
    21901 atttcccctg attttctcgt agcaaaaaaa ttcattggta aagttaacac atgtttgaaa
    21961 tactgaagca taattgccat actcattcgc tgtcctaaaa taataagaag ataactacga
    22021 ctatattcaa acaatacccg aaataaatag acagcgatca agccaatgga aattatattt
    22081 aaagttgaac gtgcttgatt agggatgaaa aaatcaagaa taccttgaaa ataatatgag
    22141 cttcctatgc caaaaaacgt aatgaaaatc gatgctaaaa cgatgtggaa tactaatcct
    22201 ttttgtttcc aaattatagg caaaaaagaa cttaatccag ctactttttc tttaatagga
    22261 atataagttt ctctaggcat aggtaataaa agaatacctg tccattcttt ttgaaacgct
    22321 tcaatcgttt ttctattttt tccattagcg ggatctgcaa tccatagtac gtttcccttc
    22381 gttttataaa ttacaacgta gtgcatatag ctgttatcaa ccaatatatg tgcaattaat
    22441 ggtaagataa gatctctttc tttccagatc tcactatctg cttgaatagc catacaatca
    22501 aaagacaatt tttcaaatgt ctttttcaat ccaaatgctg aagtaccctc taaatcagtc
    22561 ccagataggt cccgtaattt atgaatcggg atatcagttt taaaatattt taaaatcatc
    22621 gaaaggcaag caacaccaca atctttttca tcttgttgct gcacaaaagg aaatgatttt
    22681 atcacttatc taacacaact ccttctaaaa aatttttata gaaaaattct caatatagct
    22741 ttaatacagt gaaaatattg tctcataatt ccacaaaaac tttgaaaaca gttcttgaac
    22801 actaactttt tcattcctga atcaaattta ttttggaaat tttactattc aagaataaac
    22861 ttgataagtt tcaggaagat ttttactgtt aatcaaatac gtatgttaca gtagcaccgt
    22921 aaaaaatatt atttaaagaa gcgtgtttaa acatgcaaaa tgtaaaggaa ctaagtattg
    22981 tggaaatgca caaactgttg gtggaggcaa taatgcttgg ggaaaattgg ggcaagttgt
    23041 aggtggactt accacagggg cagtaggtgg tgcaggttta ggaacagcaa tttgcggacc
    23101 agcttgcggt gttgttggag ggttatatgg agctgtcgct ggaggtgctg cagctggctg
    23161 ggatgctcga aaaaaataaa aaaggagaat aagtagtatg catattaaaa acacaaaaac
    23221 aacatttata ttatcttcag aagagttgaa aaatattcaa ggtgggagtg ccgtgggagt
    23281 tttaggcact actttttctg gggcaacagc aggagtaaaa ctttgttcag ccggaggacc
    23341 ttatgcaata gctgcttgtg gtgtaggtgg agctttatta ggtgctggat tcagcatgtg
    23401 gaccggtgcg taaacaatta aaaaatatca aacctcaaga ttggtttgtg tcgattacga
    23461 acataggtat gttctttatc ttttcaaaag tttggaatgg acagaacttt ttactaaagc
    23521 ttcttgtgct agtttttata attatattaa atttttatct tttattaaag tcagataaaa
    23581 attctttgga aatgtaatgg tagagatatt tttacaaaag gacttgcagc gctgtagttg
    23641 gtgctgcagg aggacctatt tcggccgtta caggaggtat tggtaatgta cttgtcggat
    23701 gtgcattaaa gcctgttcct gcaagttaaa tttataaaaa caggaaagta attatctcaa
    23761 atcctatcaa gtacaatcat aggattgcac ttgacaggac aaatttttta actaaaaaat
    23821 gaaaaacact ttctaaaaat tagtagaatt tgccaaatct ttcgcatctt ccaccataca
    23881 tttaatttta attccccttt atggtactta ataaaaacaa gaaaagaact attcatttgg
    23941 cccttttttg gttccagacc atagaaatat tcctccagta actaatattg ctgttatgca
    24001 cgtaactaat aacagctata aaaattggta caactttaat aggattcaga aaaatttgca
    24061 ctagtgaagg aatctatttt attagaaaaa tgcaatctcc ctaaagaaaa aaccactaaa
    24121 cagtgctttc tttcaacttc tccttaattt tttgtgcttt ccctaaagta aagtttctgg
    24181 ccaactctga atcaaaatat agtgaacgtt ttttaaaatc tatttcttta acgttaaaga
    24241 tatttactaa acacgctcga ctaattcttg ttaacatagg atatttcttt tcaagctcac
    24301 taattcttcc ataaaattcg tactgcccat ctttagtaca caatgataat ctatgtggta
    24361 attttgaagc ttctacaaaa taaatgtcat ttatatcaaa agtaaatgtt tgagaaccta
    24421 tggaaaaaac aaatgcttga ttcgtaacct gttttgttgc atctatcctt tcttgcgcta
    24481 ataacaataa ctcaacaatc tcgtttcgat actcatctag tgtttgatct tttgttacaa
    24541 atcctaacgt ttcaactctt ctttttattg ttacgggtaa catctcatca tgagtagttg
    24601 taaaaatgat ttttgcttga acatcatatt ttctgattac ttctgctaat tctataccgt
    24661 taacttcatg atttaaatca atatctaaga aatatattcc ttgctttggt cggaattgtt
    24721 tgaggtattt tttaacttct aacggacttt gtgttttcaa tactatctta aatactttat
    24781 cgtgaaaaag aatgaaatta tcaataattc gttctaactg atttaattgt ataatttgat
    24841 cttcacataa tattattgga taactcataa atcctccttc cctcaaaaca acaaaataac
    24901 ttgagtagta aattgatacg gatcgttttg atagttcaca aacatattgg gattcttttt
    24961 attgatttcc tctatactag ataaacctaa cccttgatgt cccttttttg ttgttattcc
    25021 ttttttcatt agagtatcta tagagagtag tatctctcta caactattcg atatcgaaat
    25081 ttctatctgt ttatcatctc ggtaaatagc tagagacagt tctctatctt gactctctat
    25141 agatgcttca attgcattat cgagaataat tccaagcacc ctaatacagt caaatattgg
    25201 aacaggaact tcaataatag tacttggaca ttcgaattta caacaaatct tattgttatt
    25261 tgcttggtat agcttcgcta tcaacaaact tttcaaatat tcatttttta tattttttaa
    25321 ataacgatat tcaaacttat tggttagata agaattagaa tagatctcta attcttttat
    25381 ttctttttcc aatatcgaat catggttttg attagcgatt tcttttaaac tcagcaataa
    25441 atttttatag tcatgtctaa atctggcaaa tttttcttga tctttttcca gtgaatcagt
    25501 atattttttt agataaacca attcttttct tttcaattgc tgttcatatt gttctcgttg
    25561 cctaatagtt attctaataa ataaaaatag aacaacaaac atttgaatga ttaaaaatat
    25621 taagacacca aacacaaaat gatcaaatac ttgatattga tgagcaacat aagaaacaaa
    25681 taaaccaatt gtaagtaaat aaatcatgaa tatagcagta agtttagaac tggactgttc
    25741 aacaatccta tttatagcta attttctata taaccaaata aacatcagca aaaacaaaga
    25801 atcaagcatt atctggaata ctacataacc ataccctttt atcccttcaa atgaaaacat
    25861 atacgtcata accatggaag taataataga tctgaaatca tcaataagca tacataatag
    25921 taatttattt aaaagcacta tattagtttt cttcttttcc tttaatataa aataacttcc
    25981 gattataggt atagctgcgg aaacatcagt aaataattca acaaaaaaag tgagtggtat
    26041 caataaacat acaaaaagat attttctttt gttaagatca gtattagtca tataaaataa
    26101 gagaaaaaag aaaaagtttc ccacgaacat tcttaataga taagcttttg tatcgatagt
    26161 aatcaagaga tcccctcctg attttaggat atcataaaaa aaattttagt tacacttttt
    26221 aaataatgaa aaaacacatt ccactaaatt tgatgccggt tttccttgag gttttgttcc
    26281 agcacctcct ttaatttttt taagctcttg atctgaacat tgtttagcat ttagtctatt
    26341 tttttcttcc attacgattc cccctacaaa ctataaatac ttttatgtca ctgattcaat
    26401 atacctattt ttctaatcaa aaaattttga gacatattta atattataag tatcattaaa
    26461 attgagattt atctccataa tctgctcgat ataaaccatt tattccagca gttcttccaa
    26521 tttcactcaa atttcttatt aattcctctt gatattcagt gaatcttatt ttattagtat
    26581 aagcagtaaa atatagataa ttcaccaatc tatttattaa aggaccggga tctaattcat
    26641 atttttgttt ttctaatttt aaatatacct tttgtaaaac ctcaagaata tcagaatatt
    26701 taggatcttt acttatagat atatcattat ataattcatg aacaatttgc ttagcatttt
    26761 ttttcatttt agcacttccc tggaattgct ccacctaaaa aaccacctat agacattcct
    26821 gcaatacaag tagttgcctt ggcccaatcg accgtacatt tatttttagt gcaatacact
    26881 ccatttccat aatattttcc actatgagtg gtacccccat agataagttg tgtctcttta
    26941 atagacaaaa tttttaaatg tttcataatc taatcacccc taaattaatg tttttagact
    27001 attattctaa cattcattaa atttcaaaaa acaattcctt cttgaaacgt agcaaaataa
    27061 ttcctgaata agaatttaat cacttttaag tatataaact atgtatattc tttaatattt
    27121 tttgagaatg cacttcgtac ataataaact ttaatcacat tagtctttca tacctacgat
    27181 cgatctcttt gactcgcacc cataaattca taatagtttg tcggcgtctt tggacgtttc
    27241 gaagatgagc tacgcattct ctttttttat agacaaaaaa gatagagacg aacatggagc
    27301 ttccaaattt ggatatttga tccaattttg gaagctccat ttatttcgtg tctgtctttt
    27361 tattttttcc agctttcgac taattcttgg ttctctttga tccagtcttt tgctgcttgc
    27421 tgtggatctt ttccattgtt gatttccaac ataacttttt ccatatcttt ttctgaccaa
    27481 tggaaattgt ctaatacttt gtaagcttct ggctggtctt ctttcaatcc ttttcgaacc
    27541 atcgtatgga tctgctcttc gcttcccatt ccgttttcag gatcttctaa gtatttcaga
    27601 tcatatttgg caaacatcca atgaggtgtc catcctgtaa ctacaattgg ttcgtgtttt
    27661 ttgatcgctt gacctagtgc aacggtcatt gctcctgaag aagatgtctc tactttccag
    27721 tctttcaagt tgtcatattt ttggatcgtg ttttcagcag ccgtaactac accagctcct
    27781 ggctcgattc ctatgatttt cttacctgcc tgatcggtta actcatcgat cgaatttaca
    27841 tccatgtagg ctggcactgc taaaccaact tttgctcctg tcaaattggc acctaggtct
    27901 tcgacttgat ctttatattg tgcatattgt gaaccatgag ttttaggtag ccaagctgaa
    27961 accatggcat ctgactcgcc atttgaaaca gatttccaca tgattgaatt atctaatggc
    28021 gtcatcgtta catcatagcc catttgtttc aatacttcac cgactacatt tgttgaagca
    28081 acttctgtat cccattcaac ataagataag ttgatacgtt ttgtttcttt ggcagatgaa
    28141 aaactcccta taccaatcgc acctaagatc actactacta cagcaccaat gatcagtcct
    28201 tgacgttttt tacttttctg tgttgttgct cctgcagctt ttttattgtt tggctggttc
    28261 aaatgctgtg taaaacggtc aatgatgatt gctaagataa ctaatgcaac cccgttgaca
    28321 aatccattcc cgacttgtgc tctttgcaat gctgataata cgccgcgtcc taagccgggt
    28381 gcaccgatca tagaggcaat aacgaccatt gaaagtgaca gcattgttgt ttgattgatc
    28441 cctgccatga tcgtactttt agctaaaggc aattctagtt tgaatagttt ttgacgactt
    28501 gtgctcccga atgaatcaga agcttctact aattctttag gaacttgtcg aatccctaaa
    28561 ttcgtaaaac gaacggttgg tggcagagca aagatgactg aggcaaatac ccctggaacc
    28621 atcccgatac cgaaaaatgc aactgctgga atcaaataaa cgaatcctgg cattgtctgc
    28681 ataaagtcaa ggatcggttt gataatactt tgggctttac tgctttttgc catcaagatc
    28741 cctaatggta ccccaatgat gatcgagatc acactagata aaagaacaag tgtgacagta
    28801 ttcatcaaat cattccatag accttggtta taaataaacc atagaccgat taatgtgaac
    28861 aacgtaagtc ctggacgttt cttagaaata aagaaagctg ctaccgtcaa taacaagata
    28921 aacaaaggag ctggaatcac taacagtaaa tttgtaatcc ccgacatcac tgcctgtcca
    28981 atcgtttgta aaaatgaaaa taggccagaa aaagttgtcg taaaccattc tgtgatattt
    29041 tctacccagt ttgccactgg taattgatag ttcaatagat tattcatgtt cactcacctc
    29101 gcttgtttct gctaatgctt caatcacact acctcggatg acgacacctt ttaattttcc
    29161 atcctctaca actgcaagtg gagtcggtga gtcataaata atattgaaaa tatcacttac
    29221 caccatgtct ttcccaatca ccgtcacatt ttcatctagg aagtcgatca atggacgttt
    29281 ttgcttccta gcttctaacg cttgatctgc agtgatgatc cctttcaggt gtctctttct
    29341 gtcaacagcc atcagcatgc tgacttcttc attacgcatc cttgtcaatg caacgtttgg
    29401 tccatcagat tcgatattcg tcgtcaatgc aggtaccatg atgttttgag ccgtaagcac
    29461 tttggaacga tcgacttctt ccacaaattc acgtacataa tcatttgctg gattggtcaa
    29521 tatttcttca cctgttccga tctgcatgat ttcgccgtct ttcatcaagg cgattctgtc
    29581 cccaatccgt aaagcttcat tcaaatcatg ggtaatgaaa ataatcgttt tttgaacatt
    29641 ggcttgcagg tccagtaatt cgtcttgcat ttctcgccgg atcagcggat caagagccga
    29701 gaacgcttcg tccatcagca agatctcagg atcatttgct aaagctctgg ctaggccaac
    29761 ccgttgttgc atcccaccag ataactgact aggatattgg tctttaaatg aaagcaaact
    29821 agaattttct aatgcttttt ctgctttttc ttgtcgttct tctttaggta caccacggac
    29881 ttctaacccg tattctgtat tttccaagat cgttcgatgt ggaaacagac cgaagttttg
    29941 gaaaaccata ttgattttat gacgacggac ttctcgcaat tcttctttgc ttaacttcga
    30001 tacatcttct ccatcaatat agatactgcc cgaagttggc tcaatcagac gattcaatag
    30061 acggatcaat gtggatttcc cactcccgga aagccccatg atcacgaaga tttctccttc
    30121 ttttacgtca aaattcacgt catacacacc aaccgttgca ccggttttct ttaaaatctc
    30181 cgttttcggc tggtgatcat tcatcatatc taaagcggct tgtgtctttt taccaaatat
    30241 ctttgttaaa tgttttactt ctacttttac caaacaaatt ctcctttttt atgtaaattc
    30301 atttttcttt ctgaaaaaag tgaccacact atgttaacat catgtagtca ctttgtcaaa
    30361 aatgaaagaa atttgcttgt ccaaactgaa aaaaacttgg ctaaatcttt gtaaataaaa
    30421 ggtttagcca agttaatatt tttttaatat ttcacacaat cattctgcaa gctgatgctc
    30481 cgggtaaaaa aagtttgtca tacgctgtag tgtatattca tttccaataa aatgaacgat
    30541 gtcccctgcc gtaaatttag cataggggcc aggtgaaaca agcagttctt cattatgaga
    30601 aattgcgata actgtggcgc cagtcgcctg ccagatattc aactcgctaa ttgaacggtc
    30661 cagatgcatt gctttttctg ataaagataa ttcaaacgga agtaaaggat tcatcttatg
    30721 tattcgcttc gtttgattga ccagctgatc caataattga gaaaaatgat tcagctcatc
    30781 ttgttgcttc tccacacttg ccatgatttc tgcttttgta tcttggatgg attgtacatc
    30841 ttgatattgc aataggaaat cttttgcttt ttccttcgaa gcaacaaatg ccccacttcc
    30901 atgccgtact tccatgattc caagatctac cagcacattg atagccttgc gggtagtctc
    30961 aggagaagca ttgtacgtac tggcaagtgt tgagcgggca tggatcttct cacccacttt
    31021 atatcggttt tcaacgatac gttcggctaa atcaacagca atctgctggt agcgcgggcg
    31081 gacgattttt ttctttccag acattttctt tcctctttct ttttttactt tccctcatct
    31141 tactatgctc tagcaaaata agcaaaaaag gggagaatag cctcttgcaa cgactgatca
    31201 tcgcttgatc tgttcctttt ttactgggca agctgtttga cagtcatcac aattcttacc
    31261 ggattttaaa cgagtataga caattgcgcc tgcacttcca aagattaaca cacttaataa
    31321 gaatgtcgcc atcctattcc tctccttcta atgacaatat agtataaaac tggttcttgg
    31381 attttggctg acggaacaaa acataaacga gtcctatcat cagtaaaata gcaagatacg
    31441 tccctaacgc tacctgtcca ccctccaata agtgtcccaa ctgataaatc acaaaactaa
    31501 ctaaataagc taaaccgcat tgatagccta tggcgatcca agtccatttc ttggcattca
    31561 tttcccgatg gatagcccca attgccgcaa aacaaggcgc acaaagtaaa ttgaaggcta
    31621 acaatgaata agctgctgcg ggagtatatg tgtgttgtag cacttgccag atttctctgc
    31681 cattttcgga tacttccgca tgaccaaaca aaataccaaa tgtgccaata atattttctt
    31741 tagcgaccaa tcctgtaatc gctgcgactg ttccttgcca gtttccccaa ccaagcggct
    31801 gaaaaacaac tgccaggatt cgcccgaaaa aggctaagat actttggtct tcagaaacag
    31861 gttgagcata aaaattataa gaagagctga accagatcac gatactagaa acaaagataa
    31921 tcgttcctgc ttttttaaca aaagacttgc tgcggtcata ggtttgtctt aggaccgctg
    31981 accattgagg aaaatgatat gcgggcaatt ccatgataaa aggagctgta tctccagcaa
    32041 acaaacgtgt ttttttcaag ctgattccag agaaaataat tgcagtcata ccaagaaaat
    32101 aggcagaagg agctacccaa gattgatgag gaaaaaaggc ccctgctact aaggcaataa
    32161 tcggcaactt tgcagaacaa ggcatgaagg ttgtgaccat gatcgtcaaa cgacgatctt
    32221 gctcgttttc aatcgtgcga ctggccatga cgcctggcac tccacatcct gtagcaatca
    32281 gcataggaat gaaggatttt cctgaaagac cgaatttcct gaaaagtcga tccatgacaa
    32341 aagcgatcct cgccatatat ccgcagtctt ccagaaagct caagcataaa aacaatataa
    32401 tcaattgcgg tagaaatcct aacactgctc ccacgcctgc gatgatgccg ttcaagatca
    32461 gctcctgcat ccatcccgct acttgccatt cagctaacaa tctgccaaca tgttctggta
    32521 accatgtacc aaacaatgtg tcattgatcc aatctgtccc cattgttcct actgtttgaa
    32581 tagccagata atagatcagc cacatcacaa atgcgaagat cggcaaggct aaccaacgat
    32641 tcgttgcaat ctgatcgatt ttgtcactca tcgataattg gaaagaatca tttgacacaa
    32701 cacagagtgt acatagttgg gtaataaatt cataacgttc attgaccaga atcgtctcac
    32761 tatcgtcgtc caatagtttc tctgttaact ggatcagttg ctcgatctct ttttgctgag
    32821 aaactgctat ctcgtaatcc tctcttacct tctgatctcg ttcaaaatat ttcattgcat
    32881 accagcgttt cttgccgctt tgttctccta gtacatcttc aatttcatgt aaagctgctt
    32941 ctaaacgtgg atcatatgta cagggaacag gaatagaagg ccgctttttt gcagactcct
    33001 gaatcttttc gattagtttt tcgattcctt gctttttcaa tacgctgatt ggtaaaactg
    33061 gtacaccaag accataggaa agcttttcta gattgatttt ttttccttct tttttgatga
    33121 catccatcat attcagacca atgatcatag gaatgtctaa atccaataac tgcgtagtta
    33181 aataaagatt tcgttctaaa ttggtagcat caataatgtt gatgatcagt tcaggctgtt
    33241 cctgaaccaa ataatctctt gcaatcactt cttctggtgt ataaggagac aaagaataga
    33301 tgccgggtag gtcctgaata tgaatgttcg gatttttttt caacgatcct tctttttttt
    33361 ctactgttac gcccggccaa ttccctacat attgattcga accagtcaag agattgaaca
    33421 agctagtttt cccgctgttt ggatttccga ctaacgcgat attcattctt tttcctccct
    33481 ttgtatcaag attagttcgg cctctgactt tcgtaacgtc agttcatacc ctcgcactgt
    33541 cagttcgatt ggatctccca gaggggctag ttttcggata actattttta catttcgcgt
    33601 caatcccata tccattaacc gtcgcttcac tgcgccttgt ccgtggattg ctacgacgat
    33661 cccagtctct cctgctttta attgatcaag agaagtccag ctttcagagg aagcagtctt
    33721 tttattgaca aagatttgac ctagtatttc ccgacttact gccactcgat gactttttac
    33781 cagtaaaatg gcattgttgc ctgataaatt gacgacgacg attttacttc cggcaacgat
    33841 cccgatattt tgcaaatggc gttttgtttt ttcttctgtt tcgattcgtt cgacttgata
    33901 aacgaaattc tgctttgcct gcgctaaggt ctgcatattg attcctctct tgtgaaataa
    33961 atcatgtatt aaaatcattc attatatgtt ctaattttct tacgtgttac gcaaataaat
    34021 gagaatcatt ctcacataga atactctcgg taattgagaa tgtcaattat acttgtgata
    34081 ttatgagcga atctttagta ttgatagata aaaatgagaa aaaaatattt cttcatagtt
    34141 ttccacaatt ttttctctat taaatttgct attttcccac acgatacttg ttttcagctc
    34201 ttttttcaaa aacacaaaac ttagtattcc acagtttcca caacacaata tgtagtgttt
    34261 gttgtttgac ttttttacta agagttttat actatctatt atagataata agaatgtgtt
    34321 agaggaggca ctcaccatga acgtaaaaat cttttctaaa aataattgta tgcaatgtaa
    34381 gatggtcaaa cggtttttaa cagaaaatca aattgctttt gaagaagtca atatcgatga
    34441 acagcctgaa gcagtagact ggttgaaagc acaaggattc caaagtgttc ctgtcattac
    34501 ttctgatgca gaaacagtta tcggattccg tcctgatcag ttgcgtaaac ttgccagcta
    34561 acatgactaa aagaatcaaa acgtgataca tggatttgaa gggcttgaga cagcaccagt
    34621 ttttgtcgca aacttccaaa tcctttttta aagcttaaaa acaatgcaat gatttgcaat
    34681 caagcatcat caaatacgaa atgaaggttg ggcgtacgta tgagtcttaa aaatttacaa
    34741 gatgtcagtt atttcaagct gaataatgaa atcaatcggc cagtcaatgg tcaaatccct
    34801 ttgaataagg ataaagaagc tttagttgcc ttttttgagg aaaatgtcaa accaaatacg
    34861 atgacttttc tatctattat ggaaaaaatc aattacttga tcgatcaaga atatatagaa
    34921 gcagaattca ttcaattgta ttcaccatca tttattgaaa agctatatca atttttggac
    34981 gagcaacatt tttctttcaa atcttttatg gctgcttaca aattttattc tcaatatgcg
    35041 ttgaaaacca acgacggttc cgcttatctg gaaacttatg aagatcgagt agccttcaat
    35101 gcgctttact ttgcgaacgg ggatgaagaa ttagcacttg ctcttgcaga cgaaatgatc
    35161 catcaacgat atcagccagc tacaccttct ttcttgaatg ctggacgtaa acgtcggggc
    35221 gaattggtgt cttgtttctt gactcaagtc actgacgata tgaactctat cgggcgctca
    35281 atcaactctg ctttgcaatt gtcccgtatt ggtggcggcg tagggattac tttatcaaat
    35341 cttcgtgaag ccggcgctcc tatcaaagga tatgagggtg ctgcaagtgg tgtcgttcca
    35401 gtcatgaaat tattcgaaga tagtttcagc tattctaatc aattaggtca gcgtcaagga
    35461 gctggcgtgg tttatttgaa cgttttccat ccagacatta ttgctttcct ttcagcaaaa
    35521 aaagaaaatg cagatgaaaa gattcgcgta aaaactcttt ctcttggcgt gatcgtacct
    35581 gataaatttt atgaattgac acgtaaaaac gaagatatgt atttatttag tccatatagt
    35641 gtcgaaaaag aatatggtgt cccattctct tatgtaaata ttacagctga gtatgatcat
    35701 ctagttgcaa atccaaacat tcgcaaacaa aaaatcaaag cgcgtgatct tgaaaatgaa
    35761 atctcaaaat tacaacaaga atctggctat ccttacgtaa tcaatattga tacagcgaac
    35821 cgcgagaatc caatcgatgg caagatcatc atgagtaatt tatgttcaga gattttacaa
    35881 gttcaaacgc cttctgtgat taacggcaaa caggaatatg aagtattagg aacggatatc
    35941 tcatgtaact taggctcgac aaatatcgtg aatttgatgc aaagccctaa ctttggtcaa
    36001 tctgtgcgta cgatgactcg tgcattgaca tttattacag atgcatctga tatcgatgtc
    36061 gtaccaccga tccaaaacgg aaataagctg aatcatacga ttggattagg tgccatgggc
    36121 ttacacactt atcttgcaaa agaacaaatg gaatatggct cagaggattc attagacttt
    36181 acaaatattt acttcatgct gttgaattat tggacacttg ttgaaagcaa caatatcgct
    36241 cgtgaaagaa aacaagtttt ccataatttt gaaaaatcag cttatgctga cggcacttat
    36301 ttcgaaaaat acgtaactgg agaattccag ccaaaatcag aaaaagtcaa agcattgttt
    36361 gaaggcattt ttatcccaac tgctgaagat tggaatgctt tgaaacaagc agtaatgaaa
    36421 gatggactgt atcatcaaaa tcgcttggca gtagcaccaa atggctcaat ttcatatatc
    36481 aacgatacga gtgcaagtat tcatcctatc actcgcatga tcgaagaacg ccaagagaag
    36541 aaaatcggta aaatttatta cccagcgcct tatctatcca atgagacgat tccgtattac
    36601 acttctgctt atgatatgga catgcgtaaa gtaatcgatg tttatgcaat ggcacaacaa
    36661 cacgtcgatc aaggcatgag tttgacttta ttcatgcgtt ctgaaatccc agaaggcatt
    36721 tatgaatgga agacaacaac gaagcaaacg acacgtgatt tgaatatttt aagacattac
    36781 gctttccaca aaggaatcaa atccatttat tacgtacgta cctttactga tgatgcagaa
    36841 gaaatcggaa gcaatcaatg tgaaagctgc gtgatctaat atcatagata gattcgctta
    36901 ttcatatttt aattaaagga gcattctcat gtcagaaaca tattatgaag caataaactg
    36961 gaatgaaatt gaagatatca ttgataaatc cacttgggag aagctgacag agcaattttg
    37021 gctagataca cgtatcccac tttcaaatga cctagatgat tggcgtacat tgtctcaatt
    37081 ggaaaaagat acagtaggac atgtatttgg cggtttgaca ctgctagata ctgtccaatc
    37141 tgaaagtggc atggatcagt tgcgtaaaga tgtccgcact ccccatgaag aagccgtttt
    37201 gaacaatatt caattcatgg aatccgttca tgccaaaagt tattcttcta tttttagtac
    37261 actgaatacg aaaaaagaaa tcgaagaaat ttttgactgg acaaatacga atccttatct
    37321 gcaaaaaaaa gctgagcgta tcaatgaaat ctacaaacac ggtacaccgc tagaaaagaa
    37381 aattgccagc gtctttttag aaacattctt gttctattca ggtttttata ctcctcttta
    37441 ttatttagga aacaacaaat tggctaacgt agcagagatc atcaaactaa tcatccgaga
    37501 tgaatctgta catggaacct acatcggtta caaattccaa ttaggattca acgagttacc
    37561 tgaagcagaa caagaaacgt taaaagactg gatgtacaat cttctttatg agctttacga
    37621 aaacgaagaa cgttatacag aagaattata cgatccaatc ggctggacag aagaagtcaa
    37681 gacattccta cgctataatg ccaacaaagc attgatgaac ctagggatgg atccactctt
    37741 tccagatact gccaatgatg tcaacccaat cgtaatgaac gggatttcaa caggcacaag
    37801 caaccatgac ttcttctcac aagtcggaaa cggctatttg ttaggtcatg tagaagcgat
    37861 gaaagacgac gattatttga ttggattgga ataaggattt attttaattc aaaaattttt
    37921 cgaaaaataa aaagagttta gcgtacagat attttaaatg tacactgaac tcttttttat
    37981 gcttattcac ttgtttcttc cagcactttt ggactgtcta acttttccga aacatgttcc
    38041 attcttctca ataaatagat tttccggata tttaatatta tcgtcttaat cactgcatag
    38101 acaggtatgc aaatcagcat gcctaatatt cctgcgacac ttcctgctcc aatcaatacc
    38161 aagatgatcg tcaaaggatg gatattcatt gatttcccaa ataacaacgg cttgatcaaa
    38221 tttccatcta attgttggat cactaaaatc gacaacgcca tatacaaagc ttgaaatgga
    38281 gagataaaca aaccgacaat gattgctggt accgctccaa taaaagggcc gacatacggg
    38341 ataatattcg tgattccgca aatgaccgca agcaacaagc tataaggctg atcgaatatt
    38401 ctcattgcca aaaagctcat tacaccaata atcaaagcat ctaagatcgt actgctgata
    38461 taggcagata atgtttcgtt taattctttt accgtttggc gtaactcttt acgaatcgct
    38521 ttcggaaaga agtgagacaa cgcatcaata aatttgtgcc catccttgaa cataaatagc
    38581 aaaataaatg gaacggtaaa taacaaaata aaaaattgca tcataaatga aatgattttt
    38641 gagacacttg cagataaact gactaatatg atattgaaca aattactaag agataaattt
    38701 gctgatgcta attgttcttc caaattgaaa tttttaaact cttcccgctg cgccaattca
    38761 tttaaccatt tgcttgtttc atctgcatac attggcaagc tctgggtcaa ttgaattgtc
    38821 tgttccatta actgaggcac tacattcatg acagttaaaa caactaaaat gattacgacc
    38881 aaaaacgtca ataaaaaacc gaacactctc gggatccgat gcttttcgag aaaaacaacg
    38941 attggatcga acatataata gataaaacca gcaacaagca atggtaaaaa aactgccgaa
    39001 agcattttca atattggact aaaaatattc ggcatttgta aaataaagta cacgccgatg
    39061 atcgtgagta ctagctccac tgtccagaaa aataattttg attgtttaaa acgtgattgc
    39121 atacatctcc tccaaggtcc tttgatactt ctattctatc atttaacaaa gagttacgcg
    39181 ctgagatttt catttgctta aaaaaaacaa aaaaaataaa catattttaa tgagtagacg
    39241 agtacttaaa caaaaacacg gtttggagta gtgacaaaaa ttatactgca gtcccttttt
    39301 ccaaataaac ggtgttcaaa agcttgcatc ttcgtaataa gcccagccaa acgaaaaata
    39361 taaggaacta tttttatttg gctgggctta ttacttgatg taggacatct attttacaac
    39421 ctcagatcat ttattgcgca atactgacta aatgcaactt tgtgatattt ttgtgtacgg
    39481 acaactcctc gatcacatcg gcataagtga gcccttttgg aagatcaatc gtatagatat
    39541 ttttgtacac tcggtagtca tcaacgaatt ttacgtcaaa attcacatct tcgattacga
    39601 tatttttctc ttcaaaataa tcggtaagaa aatctttggt ttcttttcta tggataaatt
    39661 ggatttctaa ctttttagtc gtaggtacat ggatcacgcg tttcactact gtgagtgcaa
    39721 tagcaatccc gataaaactg atgatcgcaa tggaatagta acccatgccg atggcgatcc
    39781 ctaatccagc aattgcccat aaagaagcgg cagtcgtaag tcccgtcacc gattgtttcg
    39841 tgacgatgat cgttcccgcg cctaagaaac cgacaccact caccacttga gcaatcagcc
    39901 ttgcttcatc tgaacgaatc gttccagcaa gctgtggata ctctaaagca tctcttaatg
    39961 caccactagc gatctcaact tggatcaatg caatgataca tgctcccata caaactaaaa
    40021 tatgcgtacg catacctgct ggacgatttt tatattgtcg ttcaaaaccg attgctccgc
    40081 caaatagcat agcgatccct aaacgtaaaa taatctctgg aattgtcaat accatatcat
    40141 tcatctaaac accctccagt ccaatatatc atgtctgaaa ggagcagaca aaaaaagtgc
    40201 aatttttacc tcttacgaaa aaaggactgt gacaaaattt ttgtcatagt cctttttatg
    40261 aataaacggt gttcaatcag ctgaccctcg gtattactca acaaacggca aaatatgggg
    40321 agctattttt cctttgtctt ctctaatact caggtcaaaa cgctgatttc acaacctctt
    40381 tagcctacta ttgaaaatta caacttactt gctgcttctt catcgatgat cacaacaaca
    40441 ttgtcgtgat tttgcaatac acttgctggc aaatgattcg ttacaggtcc ttcaatcatc
    40501 cctttgattg catcagcttt gctttcgcca aatgccatca agacgatttc tttacttttc
    40561 ataattgaac cgattcccat tgaaacagct tttgttggta catcttctgc tttatcgaag
    40621 aatcgtttgt ttgcattgat tgttgattct gttaagtcaa ctactgaagt cgttccgtca
    40681 aatgaagcac caggttcatt gaaaccgata tgtccatttt gcccgattcc taagatttgg
    40741 atatcgattg gatgtgcatc gatgatttga tcatactttt tgcatgctgc ttccagatct
    40801 tctgcttttc catctggtac aaacgtttct ttgaatggtt ttttatcaaa taattggaca
    40861 tccatgaaat gacggtagct ttgttcatct gaaccgccta gtccaacgta ctcatctaag
    40921 tttacagaag tcatttcact gaaatccaaa tcgcttgaag tcatttcttt atataaagta
    40981 actggtgtgc ttcctgttgc caatcctaat acttttgcac ctttgttcat accttctttg
    41041 atgatttcaa atgctttttt tccgccttct tcagcgtttt ttacacgaat gatttccata
    41101 gtgattgctc ctttatattt tggtatagcc caattatagc attctttttt tctttttaca
    41161 ataattagta tagaccaaat tgcatttttt tcattttctt gacaataaat aggaatccgt
    41221 gttaggtttc ataagatggt tcaatcaaac aatccatgtt gcatgaaaac caagcaagtg
    41281 attcactgtg gacgctttat gtctgcagtt tttttatgaa aaaggagtta ttatgagtat
    41341 actgacatta aacaacatta cccagcaatt tggcgacaaa atcctctatg aaggtgtcga
    41401 tctgcaatta aatgctggcg aacatcttgg gctgattgga caaaacggag ccggtaaaag
    41461 tacattgatt aaaatcatca ctggtgagat cttaccagat gacggacaga tccattggca
    41521 aaaaaatatt cacaaaggtt acctcgatca atatgtggaa gtcgatgaaa cattaacaat
    41581 cgaagaattt ctaaaaactg cgtacgcaaa agaatacgaa aaggaagaaa aaattgcttt
    41641 tctttaccaa acatatagcg agaccttcga tgattccttg ctggaaaaag ctgggaaact
    41701 ccaaacggaa ttagaccaag gagtgtttta tcaaatcgac accttagttg ctgagatgag
    41761 cagcgggttg gggatcgatg tcttaggttt agattcactg ttgaaaaatc taagtggtgg
    41821 acaacgttca aaggtgattt tagcaaaact acttttagaa aacccagatg ttctgatctt
    41881 agatgaacca accaatcact tagatgatca gcatatccaa tggctgacgc agtttcttca
    41941 ggattttgac ggtgcgtata tcgtgatttc ccatgaccaa gaatttcttg atcagatcac
    42001 cacccatatc gcagacatcg agtttggaaa gattactaag tatactggcc atctaaaaca
    42061 agcattgaaa caaaaggaac aaaatcgcga aagctatttg cgccaatatc atgctcaaca
    42121 aaaacaaatc gaaaagaccg aagcttatat tcgaaaatac aaagccggtt cccgatcaac
    42181 gatggcaaaa agccgacaaa aacaattaga taaaatcgaa cggctgacgc ctccagcttc
    42241 agcaagtaaa gctgtctttg attttccatt ctcgcctatt gtcacgacat tagcagtgga
    42301 aacaactgat ttagtcatcg gatatgatcg accgctactt gcacccatca atttgaccat
    42361 ccgctttggt gaaaatattg ccattcgagg cttcaacggg attggaaaat ctactttatt
    42421 aaaaacactg atcggtgaga ttgaaaaaat aagtggcgat ttccacttcc cagataacac
    42481 aaaaatcaat tacttttcac aagatctgta ctgggaaaat ccattagaaa caccactaca
    42541 atatttaagc agtcaatttc caaaagcgac tatcaaagaa ttacgtcgcc aactagctaa
    42601 agcagggtta gttaatcaat tagcaagtga acctctcact acattaagtg gtggggaaca
    42661 aactaaggtg aaacttgcac agttgacaat gaaccaaggt aatttactga ttctcgatga
    42721 accgacgaac catatcgatc aagaaacgaa agaaagctta caagacggga tacagaaatt
    42781 tccaggaaca gtaatcatcg tctctcatga acaagaattt tatcaagatc ttgtgcaacg
    42841 agtcattgag attgaaccaa aataaacgtt tgattgaata gttgaaaaaa aggagctgtg
    42901 acaaaagttt tgtcacaact ccttcttctg aataaatggt ggccaattag ctgaccttcg
    42961 gtattagctc aaaaaacggc aaaatatgag gagctatttt tatttggcta ttcttattac
    43021 ttgatgcaga acagcttttc acaacctctt ttcgtctatt ttttattttc gagatttctc
    43081 tttcctttaa aaacaactgt ttgaaagaag aacgcacatc cttgaaaaac aagcccgcta
    43141 accgttacac ctaaccagcc atactgctgc cacattgtcg tccctagcaa tgaaccagcc
    43201 gcacctccaa taaagtacat aaacataaat acagtattat ttcggttgct tgctgcttca
    43261 cctagccctt gtactcgtgt ttgattggcg acttgaccaa attgattccc gacgtctaat
    43321 aaaataatag ctaaaagcaa aatggccaaa tggcttccgc caaccaataa agcaataaag
    43381 cttattgttt gcataacaag tcccatatac acgatttttc tttcagaata agaatctgcc
    43441 agtcgtccca cgataggtgc agcaactgct ccagacaaac caaaaatcgc taatatccct
    43501 gcttcgaatg ttccccaatt gtaggcggga ctgctgatat aaaaaatcaa cgtagaccaa
    43561 aagatcgaaa atgttccaaa catgaaaaat ccactgactg ccgcttctct taatagacgt
    43621 tgagaagcaa ctaaatgcgg caaactttta agtgaatcaa ggtatgaaac agctgtacta
    43681 ctttgctgga cagaacgcgg cattttccag taaagcagaa gtgtaagaat tccgactgca
    43741 cataaagcaa ataaatagat catccgccaa gaaaaaagac tggcaacaat accagatacc
    43801 gtccgagaaa gcaaaatccc cacgagtaaa ccgctaagca atgtacccat gaccttccct
    43861 cttgcttttg gtcccgctag taccgcaccg taaggaatga tgatttgcgg cacgatcgaa
    43921 agtaatccta ttaaaaaaga agcgcatgca aataacgtaa acgaaggcgc aaaaaaagcg
    43981 gccaacagag agacggcaga taacgctgcc attcgaatga tcaattttgg acgatccacc
    44041 acgtccccta aaggaactaa aaacagtaac cctaacgcat agcctaattg ggtcaacatc
    44101 gtcaatatcc cgactgcact tgtgctaaca gataaactag ctgctacttt tgttccaatc
    44161 ggctggatgt aatacatatt ggcaacaaca acgccacaag tgattgataa tagtaaaata
    44221 agcgaagttg ttaaaggttt tttttcatta ctcttcattg atttccccca tttcctatct
    44281 tagtttacaa cttatggaaa agattggaaa tagaaaaata caactattcg ccacacttat
    44341 tatttgtgct tttgttttcc ttgttttttc ttgcgatctt tataaatgaa gacaactaac
    44401 cctactagca cagtaacact tgcaaccaac gtgactgcag ttttgttata tccgtgaaac
    44461 agagcttctc caccacacgc atacaacaca gacgaaggga tgactccaat cacgatcgac
    44521 aatatgattt gctgtagagg caatttcatc gcatctgcag caaaattgac catagaagat
    44581 ggaataaccg gtatcatata gcctaacatt acacctattt ttggatgttt catttgtcgg
    44641 attgcttgca cccaatgatt cgtttcggtt ttcttatcta aaaattttaa gcgatgcata
    44701 aggaaaattg cagtgatgtt accaagagca ttacccaaaa tgttgatgat cgcacctgtc
    44761 agtggtccaa agctcaaacc agcaatgact ccaactactg aagtaggaat acctggaaca
    44821 gcacacatca ccgctgtcaa tacgatcaag agtatcgatg caaaagtccc atgcgaacga
    44881 atatgtgcga gtagctctgc cttactcgct ctcggattta aaaataattg gatgtctgtc
    44941 tgatattcta aatacaaccg atagcccact accagtatca gaatgattcc caacgagatc
    45001 aatatcaatc ggattatttt ctttttcata tttcctcctg aaaaaaatct atattcatta
    45061 aataatagtt taacataaaa acaaaaaagc accgaaatcc aacataggat ctcagtgctt
    45121 ttaaatagta aaaattattt aacagttaca gatgcgccaa cttcttcaag tttagctttc
    45181 aattcttcag cttcttcttt agatacgcct tctttaactg gtgcaggagc gccatcaact
    45241 acagctttag cttctttcaa gcctaaacca gttgcttcac gaactgcttt gataacttta
    45301 actttttggt ctccagctgc agttaattct acagtgaatt ctgtttgttc ttcagcagca
    45361 gcagcgccac cagcagcagc tacagctaca ggagcagcag cagatacgcc aaattcttct
    45421 tcaatagctt taactaggtc gttaagttct aggatagttg cttcttttaa ctcagcaacg
    45481 atgttttcaa tgttcaatgc cattataatt tcctccgttt aattgttctt ttttatttaa
    45541 tgctttatgc ggcaattaag ctgcatctcc atcttctttg tctgccactg ctttgacagc
    45601 gtaagccacg ttgcggactg gcgcttgtag tacagatagc aacatagata gaagtccttc
    45661 gcgatttggc aattttgcca atgctgtgat ttcttccaat gaagagactt taccttcgat
    45721 gataccgcct ttgatttcta atgcttttgc ttctttagca aattcgtcca tgattttcgc
    45781 tggtgcgact acatcttcgt tactgaatgc aacagctgta ggtccagtga atacttcgtc
    45841 catgccttca aggccagctt ttttagctgc acgtgaaaga atagaatttt tgataacttt
    45901 catttctacg ttagcatcac gaagttgttt acgtaaacgt gttacttcat caactgttaa
    45961 tccgcggtag tcaacgacta ctactgatgc agctgctgta aatttttcgc ttacttcatc
    46021 aacgattgct gctttttttg cgataattgc ttcactcatt tttgagtttc acctcctgaa
    46081 ttttgatgga agagtttttt gcactgaaaa ggcaagtcac ttttatagtt actttgctca
    46141 accagtatta ttcatcacct attcacttcg ttcatagtga ttctactcgc tttgctcgaa
    46201 aggaaactcc tttcgctttg ctcaaccagt attgttcatc atctcttcac ttcgttcata
    46261 gtgattcaca ataaaaaact ctatgccacc gcagacatag agggactatt gaatatattc
    46321 aataaacgtc ctcggtagga aattaaggct tgatgccacc tactgtcttc ggtacaagtt
    46381 aattattaac ctcaactagc ttagcatgct aaactagttg agtcaagaat tatttttcag
    46441 ttggatcaat caacattcta atatttaatt agaaagaagc ttggtcaaca tggataccag
    46501 gtccaaatgt tgttgtgatc gttacatttt tcatgtattg acctttagct gctgatggtt
    46561 tagcttttag caatacatca tggattgctg caaagttttc aactaatttt tcatcgtcaa
    46621 atgaaacttt tccgattggt acatggatat ttccagcttt gtccacacgg taagttactt
    46681 taccagcttt cacttcgttt actgcttttg ttacgtccat tgtaactgta ccagttttag
    46741 ggtttggcat taagcctttt ggtcctaata ctcgtcctag acgacctaca gtagccatca
    46801 tatcaggtgt tgcaacaact acatcaaagt caaaccatcc gttttggatt ttttgaacca
    46861 tgtcgtcgtc acctacgaaa tctgcaccag ctgcttcagc ttcttttgct ttgtctcctt
    46921 tagcaaaaac taaaacagtt tgtgttttac cagttccgtt tggtaataca acagcaccac
    46981 ggatttgttg gtctgctttt ttagggtcta cgttcaaacg gtatgcaact tcaactgttg
    47041 catcaaattt tgcaatgttt gtttcttttg ctaaagctac tgcttcggca acaggataaa
    47101 ctttacttga atcaactttt tttaatgcat cttgcatttt tttgctcttt ttagccattt
    47161 tggttcctcc ttgattgtgg ttataacggt tgtacctccc acgtgttcta tatctttcaa
    47221 tatagagcca actgagaagc tgctgtctta gggtgtatag attattctac agtgatcccc
    47281 atgcttcgtg cagttccttc aaccatgcgc attgctgctt caacgttagc tgcgtttagg
    47341 tcttccattt ttaattcagc gatttctctt acttgatcgc tagaaacttt cgcaacttta
    47401 tttttgtttg gttcgcctga acctttttcg attttagctg cttttttcaa tagaacagca
    47461 gctggcggtg tttttgtaac gaatgtgaat gaacggtctt cgtatacaga aataacaacc
    47521 ggaatgatca aacctgcttg atcagctgta cgagcgttga attcttttgt gaatcccatg
    47581 atattgatac ccgcttgacc tagtgcagga cctactggtg gagctggagt tgctttacct
    47641 gcaggaattt gcaatttaac gattttttct actttctttg ccacgagaca tacctcctta
    47701 agtccgtgat gtggttaatt ggagatgcat atttctcctc ccacgtttta tctgatctga
    47761 aaaaatccag acaaataatg tgcaaaacaa tgcacactgg tacagtatag caaatttatt
    47821 tgttttttca agattttttt ctgctatttt tacattttgc ttgaataaac gctaaattta
    47881 ttctaaaaaa acaacaattt ttcgttctca tttcttcttt tatgttacac ttgcgctagc
    47941 atggttcaac agatgaaagc aaacaataac acggtttttt agacccttgt acaaagattc
    48001 ctacataaca aaatgaggtg gagaaaatga aagatttaag gtatagaagt gtctttgata
    48061 ttattggacc tgtgatgatt ggtccaagta gctctcacac tgccggagct gctagaatcg
    48121 ggaaaatcat acgtagtata tttggcgaac agcctgattc tgtagatatt tatctatatg
    48181 aatcatttgc aaaaacctat cgggggcacg gaacagatat tgcattagtt ggcggcctgt
    48241 tagggatgga acccgatgat gaacgtttag ctgattcttt gaaattggca tatgaagccg
    48301 gtatggaagt cttgtttgtt cctaagagtg aaaaagccga acatccaaac tctgtaaaga
    48361 tgcttctaaa aaaaggaaca cgaaagcttt cagtcactgg catttcgatc ggcggtggca
    48421 atatccagat ttctgaattg aatggattca aaatttcttt aagcatgggt actcctacct
    48481 tgatcgttgt ccaccaagat gttccaggta tgattgctaa agtgactagt atactttctg
    48541 agaccaatac gaatatcggc actatgacag tgacacggga atcaaaaggt gaaaatgcca
    48601 ttatgataat tgaagtagat gatcctcaag tagaagaaac ggtaaaaaga ctcaaacagc
    48661 tgccaaatat cgacagcgta aattatttcg attaaggagc taaaaaaatg tttttatcga
    48721 ttgaagaatt agtagatcaa gcaaaagctt atccaagtgt agcagaattg atgattgcag
    48781 ttgaaatgga aatgagtggc cgttcccgta gtcacatcat cgagatcatg gaaaaaaacc
    48841 tctcagtaat ggaacaatca atcaacgagg gaaaagaagg tgttacctcc gtcacaggaa
    48901 ttaccggtgg tgatgcaaaa aaattagatg attatttaaa tgaggcagac tttttaagtg
    48961 gagaaacaat cttaaaagct gtacgcaatg ctgtagctgt taatgaagtc aatgcaaaaa
    49021 tggggttgat ttgtgctaca ccgactgccg gaagcgcggg tgttgttcct ggtgtgctga
    49081 tggctgtccg agatcggctt gatttgacac atgaacagca attggaattc ttatttaccg
    49141 ctggcgcttt cgggttagtc atcgcaaaca acgcttctat cagtggtgca gaagggggat
    49201 gtcaagcaga agttggttct gcaagtgcaa tggcagcagc agctttggtt tgtgcaaaaa
    49261 atgggactcc tcatcaagca gcacaagcag tagccattac attgaaaaat atgatgggac
    49321 tgatttgcga tcccgttgca ggcttagttg aagtcccgtg tgtcaaacgt aatgctttag
    49381 gctcttctca agcttttatc tctgctgata tggcattagc gggtatcgaa agtgtcattc
    49441 cgccggatga agtcgttgct gcaatgtacc aagtcggacg tcaaatgccg tcgattttca
    49501 aagaaacagc agaaggtggc ttagctgtta cgccaactgc taaacgactt gcggaagaga
    49561 ttttaaataa gagggcgtga taaagctcct taatttcgag tattaagaga ttagttatct
    49621 tcataacaaa aaacgaagag aggactaatt gcccttctct tcgtttttta ttaatccatc
    49681 aatttcgtat tttttacttc atcgatcgtt tttgttcctg caagttgcat cgtgatcgac
    49741 aattctttgt tcaggtgttc aaaaacagat gttactcctt ctgccccacc tagattcaac
    49801 ccataaatca ctggtcggcc aatcgcaact aagtcagcac cacttgccag agctttgaag
    49861 acatgctctc ctcgacggac accagagtcg aaaatcactg gtacacgttt gttgactact
    49921 tcggcaattt ttggcaatac ttcaaaggac gcaggacctc catctagttg acgtccacca
    49981 tgattggata cccagatacc atctgcccct gcagagatag cgattactgc atcttctggc
    50041 gattgtattc cttttacgat aactggaaga tgggtgattt ctttgattgt tttgatgtca
    50101 tctggtgtca agccttgttt tgcagctgca taaatttctg cgattccttt tccttcgcca
    50161 ttaccacttg cactttgttc gctatacgca gctaagttag gcattggtaa tggaaattgg
    50221 aattgattga tgacgtcttc ttcacggtaa ccaccgagcg ttgaatctgc cgttaaaatg
    50281 atagctttag cccctgcttc tactgctttg ttcaaaatga actcattgaa tctgtcgtct
    50341 ttactcatat ataattggaa aaactgcggt gcgcctggcg ctgcatcggc tgcgtctttg
    50401 attgtcgtat ttgcataagt gctgatggaa aagattgatc cagcagcagc tactccttta
    50461 gctgtatcag cttccccttt ttcatgtgcc aatccttggg ccgctgatgg cgcttggata
    50521 atcggtgtat ctaattcaat tccgaaaaca gatgtatgca aatcggctga gtcgattccg
    50581 cgtaatatcc gtggcatgat tttctttgta ttaaaagaag tcgtattttc tttcatcgtc
    50641 cactcatctt cagaaccacc gcgaatatag ccaaaagctc ctgcttccat gcgctctttt
    50701 acacgacctt ctagttaggc aatgtttaca atatcgattg gatgttcttc attacttgta
    50761 tgataagtca tagtagatcc ttctttcatc ttcttgatta catataaatc atactaaact
    50821 tacaaaaagt aagcaaacat ttactctcag atacttttat cgctattctt tccaaataaa
    50881 tcgacttaca agctgatcta cttcttggtt ttcatgaagc atactatgag aggcattttc
    50941 acctgtcact actttttcct ctatttcatt tccgcgttgc ttcaacaacg catagaccgc
    51001 taatgcactg tttaacggta ctgttccatc actcaagtct gtttcatcta gttgaccagc
    51061 aatcaagaaa aatcgagtgg ttataggaat attaccgatc atttgctggt aatcttgata
    51121 gcggttagaa acgacagcag gcccctttgc taactcatct gtcagagact gcgcactatc
    51181 atcgacaaaa tcattaaaag gcgcgccaat tgctgttaat gtctgaactt gtggcaatga
    51241 cgtatcctgt ccatacgttc ctaaatagcg tagtgcacta actcctccca tggaatgccc
    51301 cactatgttg actttatcta tgccgtagtt ttctttcaga tacaaaagac aagttttgat
    51361 ccattctgct tgattccact cgttattttt attatcttca aacaatactt ggaccatcgg
    51421 attgtctgct tttccagaca aatctcctgt tgcttgtatt tgtccatctg ttcctactgt
    51481 taaaaccaat tcttttactg cttgctgatt tttttccaaa cgccgcaaca tccctttaaa
    51541 ggaattttct gtcccgctgt agccgtggaa aaaaagtgtt ggaatagtgg ttttttctat
    51601 cactttactg gattctgccg ttttttcttt atcagaaact tcagtagtat ttatcttcgt
    51661 cgtctggcaa ccacttaacg caaacaaact gataaggact aaagctgctg cagttgcacg
    51721 tttgcataaa tttttgattg gttctgtcat acacctcttc ctttccatat agaaaaaaga
    51781 aagacttgaa aggccaatca tatgatcgac atttcaagtc tctttattaa tcgattttat
    51841 ctacttgatc gaaatcaagt tctgtactgg tttcacgtcc aaacatatcg atgttgactt
    51901 tcaatttttc tttttctccg tcaacttctg ttacttgtcc ttctaatcca gcaaatgcgc
    51961 cttcgatgat tttcacgact tcgccaactt ctacttctag atcattttgt cgcaggctca
    52021 taccgataga acgcaagata tgattgactt cttctggaag taatggtgct ggtttgcttc
    52081 cggctccgtg tgaaccaacg aatcctgtca caccaggtgt gttgcggaca acataccatg
    52141 aagcgtctgt catgatcatt tcaacaagta cgtatcctgg gaatgtttta tgaacgattt
    52201 ctttttcttt cccatttttg acttcagttt ctttttcttc aggaacaacg acacggaaaa
    52261 taaagtcggc cattttcata ctttgcgcac gagattcgat atttgctttt actttgtttt
    52321 catatcctga atatgtatgc aagacatacc agtttttttc aaaagattcc attgaaggct
    52381 cctcatttca ttgtaaaata aaaaaacctc agtacacccg aagtttttct ttcgcttcat
    52441 tatagcacta aaagtacgtt gtggctagcg agttatttta aaatccagcc aaataagctt
    52501 tggatggctg tatccattac gtaaaacatc aatccgaaaa taatagaagt ttcgatgact
    52561 actaacgtat ctttacggag ttgttttctg cttggccaag ttacttgttt gatttcatct
    52621 tttacgcttt ttagaaattt catgagacaa cctcccttgc tactgtttat ttcgtttctt
    52681 tatgtaatgt atattgatta caatatttac aaaacttatt gatttccaaa cgttcgccgc
    52741 tttttccttc actgactgat tttgtgtagt tgcgagaccc acaaacggaa caagcgagtg
    52801 ctgctttttt ctttgccatt tacttctgcc cccttgctga gaatcagact tatacttcat
    52861 aacagtatca cgtaactttt tacttgtcaa tttgtgaaat aaactatcta ttgttttgga
    52921 taaaattatg atatgattaa tttgaaatta agctaaaatg agacaattgg aggtatagcg
    52981 atgctattaa aaacacttgt atataagaaa aaagatctga caaccgttac agagactagt
    53041 acattagaag aagcattaaa aattttagaa acttccggat accgttgtgt gccgatctta
    53101 gattcttcag gtaatatttt ccgtggaaac atttacaaaa tgcatatcta tcgtcataaa
    53161 gcaaatggcg gtgatatgac gcttcctgtc actcatcttt tgaaaaacgc tacaaagttc
    53221 atttacctgg atacttcttt cttcaaagta ttctttacga tcaaagagct tccttatatt
    53281 gctgtccttg acagtgaaaa tcatttttat ggtattttga cacatagtac gttgcttaat
    53341 atgctctctc aatcttggag cgtagaacaa ggaagctatg tactgacaat tgcttccaca
    53401 ggaaagcaag gagatttggc tgctattgca aaaatcattt caaaacacag cagcattgcc
    53461 agctgtatta ctcttgatat cgaaaaagac gaatacattc gacgtacatt gatcacgcta
    53521 ccggctaaca ctgaagcgaa agtatgtgaa acgatcgtca accacttaga gaaaaagaat
    53581 tataaagtgg tagaaattga aaatttgcaa aaccaagaag cataatcaaa aagcgaaaaa
    53641 agaaaacagc taatctgctt tcttttttcg ctttttataa ttagacacgc ttttcaaaat
    53701 caagcgcgcg atctaaataa tacatcaatg gtgctgaaac agccatgacg ataaactcac
    53761 ttaatgcaag cgtgccatag gttgtccaaa atgcttggcc acctgttgga actaacatat
    53821 aagcaatcag tcccatgctg atggtaaaga agatcgtgtt caatactaaa cgcatcttta
    53881 catttttcac tttgttttgc aataaagctg tcaggcctaa agaaagcaat gtttggccgc
    53941 caccaaaaac cacatccatg ataccaaaac caaagaaggc attatagacg atgaccccgc
    54001 ctaaaactcc ccaaagcaat ttacggttaa agacaactaa atgattcaaa ctttctgaaa
    54061 tacgaaactg gatcggccct gaggatacag gcgcaactaa aatagttaac gctagataaa
    54121 gagccatgat gatgccattt acaacgatta cggataaccg ttgtctgctt ttcgtttttt
    54181 gcattttctc tcctcttttc tcctagtttt tttaacgcgg gatggttgat gaaccgcggt
    54241 tatgtgaaac aaacaataat tataacagac ttttcattta tttcaatccg tcttatctgc
    54301 ttttaaaaaa gcaaaagctt aaacaatgta aacgtttgct aaaaatcgtg ggatatggta
    54361 taaagtaagt gtaaggggtc ataagaattc ccagtaaagc agacgaagta ggatctgcaa
    54421 gaacagaatt gttgtgaaac ttaaataaat aaaaaaggat gtgggaattt gagcaacctc
    54481 ttactagaga acggtctagc aagcagctga agatctaagt ggacatgccg aaccttggaa
    54541 cttctcctta cgtaagtaat ggatcatgcg cttgctaggc ttttttgtgt ttttcggtct
    54601 tcggaccttt ttcattttcc tagacttctg ctataataac caagaattaa ttttgtaaca
    54661 gatttttttg aaaaaacaag tttctttcat tcaaaaaata gaaaaagctt gctaattatt
    54721 ggttaaccag caaacaggag tattaaaata tcttgtttta ttgaatagca ggaggaaatc
    54781 atgaaattcc attatcatat attgatcaat ccatcggcag gcagcggaaa tggctataaa
    54841 gtagcagaaa gaattttacc ggtattaaaa aataagcata ttgactatac acttcattat
    54901 agtgaataca aaggacacga tgctgagatt gctgaaactt tggcaaaaga aacattgatt
    54961 ccttggatag aagaagaaca gaaaacaatc gatactttcc cccttttgat catcgtagga
    55021 ggtgacggca ccttacatca ggtacttgat actttctatc agatggaagt agaattccca
    55081 gttgcttata ttccagcagg ttctggagat gattttgctc gaggggcagg ccttccaaaa
    55141 aatcccaaaa aaggacttca gctgatttta gcagctcaat caccagaaaa agtccacata
    55201 atggcttatg aagaaaaaat aagtgaaaaa aaaggaatag ctgttaataa tttcggtata
    55261 ggattagatg cagctatcgt ccatgcgaca aatcattctt ccacaaaaaa gcgattgaac
    55321 aaatacaact taggatcatt ttcgtattta ttctctattc tccgtgcttt attcactcaa
    55381 aaaggatttc caattctggt agatgcagga ggaaaacaat taagtttttc aaacgcattt
    55441 ttatgtacag caacgaagca tccttttttt ggcggaggaa tcgcgattgt tccaactgct
    55501 gatgcgtcta aaccagtcat tgattttgtc gtggtcgaac gaatcaattt ctttaaaatc
    55561 ttgtggctca ttcttttact tatacagaaa aaacaattga aatcgaaata tttccatcac
    55621 tacacaacaa acagattacg tatcgtttca acaacaccgc aacatggtca agaagatgga
    55681 gaagaaatgg aaaaacgacc ttttgatatc acaataacca caactaaaca gttgttttgg
    55741 tgttaataaa agaggttttg tcagaagcta catctggaac accgtttatt cggaaaaaag
    55801 gactgtgaca cgacttttgt cacagtcctt tttttactat gcagttaatt ctttgcccaa
    55861 ttgggttgcc atcgatagtt tctttcttta cccaaaataa attggatcat tgaaaacacc
    55921 gcagcaaaag caactaagac acaaccattg aaccaaaaag attctgcaaa tgtttgattg
    55981 taactgagtg tttcactgat caagttatta ttttgaacaa accaatatgt tggtgtgaat
    56041 gccgctattt tgctaaccat ttctggcaag tattctgctg gtacaaacac cccaccgata
    56101 aagcaactgc ctgtgataaa aatatctttg atcccaccgc tggtttctga gttttttacc
    56161 aatgaagtga tacaagccga aaagctaatg atcggcaata aaaacaagaa agtatttaaa
    56221 atatgatacc ataccgttat atcccacgag gtgtggatca ctaacagaac cattagaagt
    56281 aagaagccac tgaacaacag cagcgagtaa ctaatcaaac taaaggttac tttgcgattc
    56341 aagtttcgtt tggtaatggg cgcactctgg tttcgtttac tgatctcttt gcgattaaat
    56401 gctagattga tgacgctgaa accgctaaaa atggatagaa acaacccata agacaagaaa
    56461 ttaaaggttg tgcccgtcaa cagctttttc atccgctgac tatatcccgc acttagctca
    56521 acttctccag taaccgagag tgccatttgt gtctgttcca tagctgcatc gattgtttta
    56581 tcttcttctt gaaacagaat cagtgtttgc aaaaaggtgt tcacttgctg tgtaacaact
    56641 gttttagaaa aggcatctgg gcgactctgt atcttgatcg tcggcatctt tcctacttcg
    56701 acttgttcgg taaagtctgc aggcagatgt aagataatgt ttacacgatt aaaataaaga
    56761 gcatcatcga tttctttttg tgaggttcca tccaatgtaa cgactgtttc cttgtcattc
    56821 aaatatttac gaaggccatc gactacaggc gactcttctt cactaatcaa ggcgattttc
    56881 gcatcttgca gagaggtatt ttcaacatca atattttgcg caaaaaagaa tgtggcaaca
    56941 acagtgataa ttacacctat cagtaaattc gatttatttg ctttaagcaa tgtccaaatc
    57001 gttttataaa gcgtcataac gcacccctct ttccatccaa gcatttccta acgcaaaaag
    57061 gaaaataaat ccgatcaacc atagaagatt ggtataaaaa ctagccaaat cagtgtagaa
    57121 ataaagtt
//
LOCUS       DACDXE010000011        56523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.011, whole genome shotgun sequence.
ACCESSION   DACDXE010000011 DACDXE010000000
VERSION     DACDXE010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.011"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWM23_000644"
     CDS             complement(79..765)
                     /gene="gpmA"
                     /locus_tag="IWM23_000644"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9883311.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(937..1518)
                     /locus_tag="IWM23_000645"
     CDS             complement(937..1518)
                     /locus_tag="IWM23_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9883312.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWM23_000646"
     CDS             complement(1651..2334)
                     /gene="rpiA"
                     /locus_tag="IWM23_000646"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAP9883313.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            2653..3066
                     /gene="rpsL"
                     /locus_tag="IWM23_000647"
     CDS             2653..3066
                     /gene="rpsL"
                     /locus_tag="IWM23_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAP9883314.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            3150..3620
                     /gene="rpsG"
                     /locus_tag="IWM23_000648"
     CDS             3150..3620
                     /gene="rpsG"
                     /locus_tag="IWM23_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAP9883315.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            3714..5801
                     /gene="fusA"
                     /locus_tag="IWM23_000649"
     CDS             3714..5801
                     /gene="fusA"
                     /locus_tag="IWM23_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9883316.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            5973..7160
                     /gene="tuf"
                     /locus_tag="IWM23_000650"
     CDS             5973..7160
                     /gene="tuf"
                     /locus_tag="IWM23_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9883317.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWM23_000651"
     CDS             7567..7875
                     /gene="rpsJ"
                     /locus_tag="IWM23_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAP9883318.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            7906..8535
                     /gene="rplC"
                     /locus_tag="IWM23_000652"
     CDS             7906..8535
                     /gene="rplC"
                     /locus_tag="IWM23_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAP9883319.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            8563..9186
                     /gene="rplD"
                     /locus_tag="IWM23_000653"
     CDS             8563..9186
                     /gene="rplD"
                     /locus_tag="IWM23_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAP9883320.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            9186..9476
                     /gene="rplW"
                     /locus_tag="IWM23_000654"
     CDS             9186..9476
                     /gene="rplW"
                     /locus_tag="IWM23_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAP9883321.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            9515..10348
                     /gene="rplB"
                     /locus_tag="IWM23_000655"
     CDS             9515..10348
                     /gene="rplB"
                     /locus_tag="IWM23_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAP9883322.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            10391..10669
                     /gene="rpsS"
                     /locus_tag="IWM23_000656"
     CDS             10391..10669
                     /gene="rpsS"
                     /locus_tag="IWM23_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAP9883323.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            10692..11039
                     /gene="rplV"
                     /locus_tag="IWM23_000657"
     CDS             10692..11039
                     /gene="rplV"
                     /locus_tag="IWM23_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAP9883324.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            11053..11709
                     /gene="rpsC"
                     /locus_tag="IWM23_000658"
     CDS             11053..11709
                     /gene="rpsC"
                     /locus_tag="IWM23_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAP9883325.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            11712..12146
                     /gene="rplP"
                     /locus_tag="IWM23_000659"
     CDS             11712..12146
                     /gene="rplP"
                     /locus_tag="IWM23_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAP9883326.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            12136..12324
                     /gene="rpmC"
                     /locus_tag="IWM23_000660"
     CDS             12136..12324
                     /gene="rpmC"
                     /locus_tag="IWM23_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAP9883327.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWM23_000661"
     CDS             12349..12615
                     /gene="rpsQ"
                     /locus_tag="IWM23_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAP9883328.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            12673..13041
                     /gene="rplN"
                     /locus_tag="IWM23_000662"
     CDS             12673..13041
                     /gene="rplN"
                     /locus_tag="IWM23_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAP9883329.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            13078..13386
                     /gene="rplX"
                     /locus_tag="IWM23_000663"
     CDS             13078..13386
                     /gene="rplX"
                     /locus_tag="IWM23_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAP9883330.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            13414..13953
                     /gene="rplE"
                     /locus_tag="IWM23_000664"
     CDS             13414..13953
                     /gene="rplE"
                     /locus_tag="IWM23_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAP9883331.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            13972..14157
                     /locus_tag="IWM23_000665"
     CDS             13972..14157
                     /locus_tag="IWM23_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAP9883332.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            14193..14591
                     /gene="rpsH"
                     /locus_tag="IWM23_000666"
     CDS             14193..14591
                     /gene="rpsH"
                     /locus_tag="IWM23_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAP9883333.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            14623..15159
                     /gene="rplF"
                     /locus_tag="IWM23_000667"
     CDS             14623..15159
                     /gene="rplF"
                     /locus_tag="IWM23_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAP9883334.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            15320..15676
                     /gene="rplR"
                     /locus_tag="IWM23_000668"
     CDS             15320..15676
                     /gene="rplR"
                     /locus_tag="IWM23_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAP9883335.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            15697..16197
                     /gene="rpsE"
                     /locus_tag="IWM23_000669"
     CDS             15697..16197
                     /gene="rpsE"
                     /locus_tag="IWM23_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAP9883336.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            16212..16391
                     /gene="rpmD"
                     /locus_tag="IWM23_000670"
     CDS             16212..16391
                     /gene="rpmD"
                     /locus_tag="IWM23_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAP9883337.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            16435..16875
                     /gene="rplO"
                     /locus_tag="IWM23_000671"
     CDS             16435..16875
                     /gene="rplO"
                     /locus_tag="IWM23_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAP9883338.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            16875..18170
                     /gene="secY"
                     /locus_tag="IWM23_000672"
     CDS             16875..18170
                     /gene="secY"
                     /locus_tag="IWM23_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAP9883339.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            18232..18879
                     /locus_tag="IWM23_000673"
     CDS             18232..18879
                     /locus_tag="IWM23_000673"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAP9883340.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            19070..19288
                     /gene="infA"
                     /locus_tag="IWM23_000674"
     CDS             19070..19288
                     /gene="infA"
                     /locus_tag="IWM23_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAP9883341.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWM23_000675"
     CDS             19321..19437
                     /gene="rpmJ"
                     /locus_tag="IWM23_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAP9883342.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            19455..19820
                     /gene="rpsM"
                     /locus_tag="IWM23_000676"
     CDS             19455..19820
                     /gene="rpsM"
                     /locus_tag="IWM23_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAP9883343.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            19848..20237
                     /gene="rpsK"
                     /locus_tag="IWM23_000677"
     CDS             19848..20237
                     /gene="rpsK"
                     /locus_tag="IWM23_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAP9883344.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            20317..21255
                     /locus_tag="IWM23_000678"
     CDS             20317..21255
                     /locus_tag="IWM23_000678"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAP9883345.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            21283..21663
                     /gene="rplQ"
                     /locus_tag="IWM23_000679"
     CDS             21283..21663
                     /gene="rplQ"
                     /locus_tag="IWM23_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAP9883346.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            21935..22774
                     /locus_tag="IWM23_000680"
     CDS             21935..22774
                     /locus_tag="IWM23_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9883347.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            22750..23619
                     /locus_tag="IWM23_000681"
     CDS             22750..23619
                     /locus_tag="IWM23_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9883348.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            23609..24406
                     /locus_tag="IWM23_000682"
     CDS             23609..24406
                     /locus_tag="IWM23_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAP9883349.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            24609..25355
                     /gene="truA"
                     /locus_tag="IWM23_000683"
     CDS             24609..25355
                     /gene="truA"
                     /locus_tag="IWM23_000683"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9883350.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(25688..28357)
                     /locus_tag="IWM23_000684"
     CDS             complement(25688..28357)
                     /locus_tag="IWM23_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9883351.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(28595..28783)
                     /locus_tag="IWM23_000685"
     CDS             complement(28595..28783)
                     /locus_tag="IWM23_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAP9883352.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            29053..29415
                     /locus_tag="IWM23_000686"
     CDS             29053..29415
                     /locus_tag="IWM23_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9883353.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            29467..31149
                     /locus_tag="IWM23_000687"
     CDS             29467..31149
                     /locus_tag="IWM23_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAP9883354.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            31266..33302
                     /gene="recG"
                     /locus_tag="IWM23_000688"
     CDS             31266..33302
                     /gene="recG"
                     /locus_tag="IWM23_000688"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAP9883355.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            33351..34352
                     /gene="plsX"
                     /locus_tag="IWM23_000689"
     CDS             33351..34352
                     /gene="plsX"
                     /locus_tag="IWM23_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAP9883356.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            34474..34716
                     /gene="acpP"
                     /locus_tag="IWM23_000690"
     CDS             34474..34716
                     /gene="acpP"
                     /locus_tag="IWM23_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9883357.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            34984..35988
                     /locus_tag="IWM23_000691"
     CDS             34984..35988
                     /locus_tag="IWM23_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883358.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            35989..36933
                     /locus_tag="IWM23_000692"
     CDS             35989..36933
                     /locus_tag="IWM23_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883359.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            36933..37895
                     /locus_tag="IWM23_000693"
     CDS             36933..37895
                     /locus_tag="IWM23_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883360.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            37922..38836
                     /locus_tag="IWM23_000694"
     CDS             37922..38836
                     /locus_tag="IWM23_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883361.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            38862..40643
                     /locus_tag="IWM23_000695"
     CDS             38862..40643
                     /locus_tag="IWM23_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9883362.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            40991..41677
                     /locus_tag="IWM23_000696"
     CDS             40991..41677
                     /locus_tag="IWM23_000696"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAP9883363.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            41697..45278
                     /gene="smc"
                     /locus_tag="IWM23_000697"
     CDS             41697..45278
                     /gene="smc"
                     /locus_tag="IWM23_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAP9883364.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            45287..46096
                     /locus_tag="IWM23_000698"
     CDS             45287..46096
                     /locus_tag="IWM23_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9883365.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            46107..47105
                     /gene="ftsY"
                     /locus_tag="IWM23_000699"
     CDS             46107..47105
                     /gene="ftsY"
                     /locus_tag="IWM23_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAP9883366.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            47292..49559
                     /gene="gshAB"
                     /locus_tag="IWM23_000700"
     CDS             47292..49559
                     /gene="gshAB"
                     /locus_tag="IWM23_000700"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAP9883367.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(49648..50100)
                     /locus_tag="IWM23_000701"
     CDS             complement(49648..50100)
                     /locus_tag="IWM23_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAP9883368.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            50250..51200
                     /locus_tag="IWM23_000702"
     CDS             50250..51200
                     /locus_tag="IWM23_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9883369.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            51193..52152
                     /locus_tag="IWM23_000703"
     CDS             51193..52152
                     /locus_tag="IWM23_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAP9883370.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            52149..52904
                     /locus_tag="IWM23_000704"
     CDS             52149..52904
                     /locus_tag="IWM23_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883371.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            52927..53880
                     /locus_tag="IWM23_000705"
     CDS             52927..53880
                     /locus_tag="IWM23_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9883372.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(54403..54699)
                     /locus_tag="IWM23_000706"
     CDS             complement(54403..54699)
                     /locus_tag="IWM23_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAP9883373.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            55054..55410
                     /locus_tag="IWM23_000707"
     CDS             55054..55410
                     /locus_tag="IWM23_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9883374.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            55420..56319
                     /locus_tag="IWM23_000708"
     CDS             55420..56319
                     /locus_tag="IWM23_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAP9883375.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
BASE COUNT        18544 a        10222 c        12115 g        15642 t
ORIGIN      
        1 agttggcagg agaaaaccgt ttatacgaaa aaagggactg tgacaaatcg tgtcacagtc
       61 ccttttttat agtgtttttt ataggtagta ttttttagca actgttaagt catcatttaa
      121 ttcataaaca agtggttgac ctgttggaat ttcaagatcc atgatgtctt cgtcagagat
      181 accttcgata tgtttagcta gggcgcgtaa tgagttacca tgtgcagcta ctaaaacagt
      241 tttgttgtct agtaatgctg gagcaatttc atcttgccag aatggcaaag cgcgttctaa
      301 tgtcactttc aagttttctc caccaggaat atcgcgtttg tctaacattg cgtaacgacg
      361 gtcatttgct gcagaacctt cgtcagtttc ttccattaat ggaggaagag tatcgtaaga
      421 acgacgccag atgtgcactt gttcgtcgcc gtatttttca gctgtttctt ttttgttcaa
      481 accttgtaat ttaccatagt gacgttcgtt taagcgccat gatttgattt gtggtaccca
      541 aagttgatct gaatattcta agatcaagtt acatgttttg attgcacgag ttaaaacaga
      601 agtgtaagca acatcaaatt cgattccagc ttctttgatt ttgcgtccgc cttcttttgc
      661 ttcttcgatt ccttctggtg ctagatttac atcagcccaa ccagtaaatt ggttcaatgc
      721 attccattca ctaagaccgt gacgagaaaa aactaatttt ggcatgttta gctctccttt
      781 taagataggt ttatatttct gaaattttca gttctgtttt attgtacaat tttttggaag
      841 agatttccat cgaaaaatta gataagattc tatgagaaaa caagtagaga atataaaaaa
      901 acgaacagtg cccacgcaac tgttcgtttt taggtattag atgcataatt gctcgatttc
      961 tttttcaggt aaattgatat atttataaca tgttccaact gaaacaccgc attttgaaga
     1021 aataaattgg atcgtttctt ttttttcata atagagacgt cggatttttt tgatcgtttt
     1081 tgcatcgatt tttggtcttc cgccgatttt tcctttttta cgagcttcat ctaatccaac
     1141 taatgtacgt tctttgatca atgcgcactc catgtttgct aggttttcca ttaattggaa
     1201 atagattttt cccatttcgc gcgtatcgat tccttcatca agactaatca agtgcagccc
     1261 gcgatctttg aataattgcg tgagttctgt taattgccgc gtagatttgc ctaatcgatg
     1321 aagttggcaa atgactagtg catcgccttg ttgcatgtca gcgatcactg actccaaatg
     1381 aacagcagta gaatcatttt gttccacttc atatgtctca tggaaaattt cttgacaacc
     1441 aaagtcagat aattgatcaa cctgagttgt taaatcaacg tcatttatgg ttgtgcgtgc
     1501 ataaccaata attttcatgg tgattccttt ctagcagtga ttttctaact ggatataaca
     1561 gtaaaaatgt agcataaagt acgtcaaaaa gatagttttt tcgcaaattt tccgctgtct
     1621 ttttttattg gatgatcaag cagtttttct ttatttacga cggattgcgt caagaatctc
     1681 aacgcctttt tctgagccga caactacttt agaaaccatg tttaggaaaa gcccatgttc
     1741 tactacccca acaagttcat ctaaataggt agctaagctt tctggctgtt cgatgacttc
     1801 tagatgcaga tcaatgatgt aatgaccacc atctgtggtt aagacttctc cgttttcgtc
     1861 tgtacgcaag acgggttgga atcttttttc ttcaaaaata tgaagtagct gttgggagcc
     1921 ataagggatc acttcaactg ggagtgggaa cttcccaagt ttatcgacta atttagaaga
     1981 atcaacgatc caaatcgttt cttttgagta cgtagcgact attttctcga aaagcaaagc
     2041 agcgccgccg cctttgatcc cttgaaaatc agcactgatt tcatcagcac cgtctattgt
     2101 caaatcgact acggggactt catcaatact ctttaacggt attcctaatt tttccgcttg
     2161 ttcttttgtc gcatttgaag tagtaactcc tgtgatacgt aaaccttctt cacgcatacg
     2221 gcgaccaatc tcttctacca tataataggc ggttgaaccg gtcccgagac ctacgatcat
     2281 cccgtccttt acgaatttcg cggcttcaat gccagctaat tgttttaaat tcatctttgc
     2341 gtccaccttt ttttagtttg agcatcgtgc tttcctcact attttagcgt gatttacggt
     2401 aaaaagatac cattcttttt tattccaata aagctttttc aaaaaaaatt gcatctcttg
     2461 ttgacatgtt ttttcattcg tgatattcta acaaaggtgc tttatctaca ggtttctgtg
     2521 aagaaacgtg ctgactgttt atcaaagcat atttgataag gatgcagaca ttgcatttat
     2581 tttttatcac aaaaaaagaa ccacctggat gtgtggaact agacgcaaat cgtgggaagg
     2641 aggaaacaag gaatgcctac aattaaccaa ttagtacgta aacctcgtaa atcaaaggtg
     2701 gaaaaatcta attcacctgc tttgaacaaa ggatataaca gctttaagaa aactcaaact
     2761 aacgtaaact cacctcaaaa acgtggggta gcaactcgtg tgggtacaat gacacctaaa
     2821 aaaccgaact cagctttacg taaatatgca cgtgttcgtt tgtcaaactt aattgaagtt
     2881 actgcttata tcccagggat tggtcataac ttacaagaac acagtgttgt attattacgc
     2941 ggtggtcgtg taaaagactt accaggggta cgttaccata tcgttcgcgg tgctttagat
     3001 actgctggtg ttaacgaccg taaacaaagc cgttctaaat acggaactaa aaaacctaaa
     3061 gcttaatttt aattattaaa caaatgaaac ttgcttctac tatatagaag taattactat
     3121 gaacaatatt tcggaaggag gagttacgga tgccacgtaa aggtcctgtt gcaaaacgtg
     3181 atgttttacc agatcctatt tataactcaa aattagtaac tcgcttgatc aaccgtgtaa
     3241 tggttgacgg aaaacgcggg attgcagcta acattatcta taatgccttt gacttgatca
     3301 aagaatctac aggtaacgat ccattagaag tgtttgaaca agcaatgaaa aacgttcaac
     3361 ctgttcttga agttaaagca cgtcgtgttg ggggttcaaa ctaccaagta ccagttgaag
     3421 ttcgccctga acgccgcgtt actctaggtc ttcgttgggt agtaaactac gctcgcttgc
     3481 gcggtgaaca cacaatggaa gaacgcttag caaaagaaat catggatgct gcaaacaaca
     3541 caggtgcttc tgttaaaaaa cgtgaagaca ctcataaaat ggctgatgcc aaccgtgcgt
     3601 ttgctcacta tcgctggtaa gattctctct gtttggtcgg cttttataag tcgacctcag
     3661 atagtgtcct tatttttagc aactagatag taaagagagg agaaacaagg agaatgacca
     3721 gagaattttc tttacaaaac actcgtaata tcggtatcat ggcccacatc gatgctggta
     3781 aaacgactgc tactgaacgt atcttgtact atactggtaa aatccataaa attggtgaaa
     3841 ctcatgaagg agcttcacaa atggactgga tggaacaaga acaagaacgt ggtattacga
     3901 tcacttctgc tgctactaca gcacaatgga agggtcaccg aatcaacatc atcgacacac
     3961 caggacacgt agacttcaca gttgaagttg aacgttcatt gcgtgtactt gatggtgctg
     4021 taactgttct tgatgcacaa tcaggtgttg aacctcaaac tgaaacagtt tggcgtcaag
     4081 caacaactta tggtgtacct cgtatcgtat ttgcaaacaa aatggacaag atcggtgctg
     4141 acttcttata ttcagtatca actttacatg accgtttgca agcaaacgct cacccaatcc
     4201 aattaccaat tggtgcggaa gacgacttca caggaattat cgaccttgta acaatgaagg
     4261 ctgaaatgta tacaaacgat cttggaacag agatcgaaga aacagaaatt cctgaagaat
     4321 accgcgaatt agctgaagaa tggcgcgaaa aattggttga agctgtagct gaaactgatg
     4381 aagaacttac tttgaaatac ttagaaggtg aagaaatcac tgaagctgag ttaaaagaag
     4441 gtatccgccg cgcaacagtt aacgtagaat tctacccagt actatgtggt tcagcattta
     4501 agaacaaagg ggttcaatta ctattggacg ctgttcttga ttacttgcca tcaccgttag
     4561 atatcccagc tatcaaaggg attgacccta aaactgatga agaagtagaa cgtcctgcag
     4621 acgacagcgc tcctttctca gctttagcat ttaaagttat gactgaccca ttcgtaggtc
     4681 gtttaacttt cttccgtgtg tactctggtg ttttacaatc aggttcatac gtacaaaacg
     4741 caacaaaagg caaacgtgaa cgtgttggtc gtatcctaca gatgcatgcc aactctcgtt
     4801 cagaaatctc tgaagtttac gctggagata tcgctgctgc tgttggtttg aaagacacaa
     4861 ctactgggga cacactatgt gatgaaaaga gtctagttat tcttgaatcc atggaattcc
     4921 cagaaccagt tatccaagtc gctattgaac ctaaatcaaa agctgaccaa gataaaatgg
     4981 gtgtggcttt acaaaaacta tctgaagaag atcctacttt ccgtgctgaa acaaacgtgg
     5041 aaacaggcga aacaatcatc gctggtatgg gtgaacttca cttagatatc atcgttgacc
     5101 gtatgcgtcg tgaattcaaa gttgaagcta acgtaggtgc gcctcaagta tcttaccgtg
     5161 aaactttccg tgctggcact caagccgaag gtaagtttgt acgtcagtct ggtggtaaag
     5221 gtcaatacgg tcacgtatgg attgaattta caccaaatga agaaggtgct ggtttcgaat
     5281 ttgaaaatgc gatcgtcggt ggggttgttc ctcgtgaata cattccagct gttgaaactg
     5341 gtttgaaaga tgctatggaa aatggtgtct tagctggcta tccattagta gatatcaaag
     5401 ctaaacttta cgatggttca taccatgatg tcgattcaaa tgaaactgcc ttccgtgttg
     5461 ctgcttctat ggcattacgt gcagcagcta agaaagctaa tccagtaatc cttgaaccaa
     5521 tcatggctgt agaagttgtt atccctgaag attatttagg tgatgttatg ggtcacgtaa
     5581 ctgctcgtcg tggacgtgtt gaaggtatgg aagctcgcgc tggcggccaa caagttgttc
     5641 gtgcgatggt tccacttgct gaaatgtttg gttatgctac aactcttcgt tctgctactc
     5701 aaggtcgtgg tacatttaca atgacatttg accactacga agatgtacca aaatcagtac
     5761 aagaagaaat cattaagaaa aacggcggaa aagctgaata atttcttaaa accggatagt
     5821 cctgcaaaga gattatccaa agagactata tttttaatat agtttcgtgt aaaataatta
     5881 tcgatgatat gggcatgtgg tgtaccttgt gtacatcata tacctatcag acaaataaaa
     5941 aatatattgt atttttagga ggacatttta aaatggcaaa agaaaaattt gaccgttcta
     6001 aaccacacgt taacattggt actatcggac acgttgacca tggtaaaact acattaacag
     6061 ctgcaatcac aactgtacta tctaagaaaa acggcggcca agctatggct tacgatcaaa
     6121 tcgatggtgc tccagaagaa cgcgaacgcg gaatcacaat ctcaacagct cacgttgagt
     6181 atgaaacaga tacacgccac tacgctcacg ttgactgccc aggacacgcg gattacgtta
     6241 aaaacatgat cactggtgct gctcaaatgg acggagctat cttggtagtt tctgctgctg
     6301 acggcccaat gcctcaaact cgtgaacaca tcctattgtc tcgtcaagtt ggtgttcctt
     6361 acatcgttgt attcttgaac aaagtagaca tggttgatga cgaagaatta ctagaattag
     6421 ttgaaatgga agttcgtgac ctattaacag aatacgaatt ccctggtgac gatgttcctg
     6481 tagttgctgg atcagctttg aaagctctag aaggcgacgc ttcatacgaa gaaaaaattc
     6541 ttgaattaat ggctgcagtt gacgaataca tcccaactcc agaacgtgac aacgacaaac
     6601 cattcatgat gccagttgaa gacgtgttct caattactgg acgtggtact gttgctacag
     6661 gtcgtgttga acgtggacaa gttcgcgttg gtgacgaagt tgaagttgtt ggtattgctg
     6721 aagaaacttc aaaaacaaca gttactggtg ttgaaatgtt ccgtaaattg ttagactacg
     6781 ctgaagctgg agacaacatt ggtgctttac tacgtggtgt tgcacgtgaa gacatccaac
     6841 gtggacaagt tttagctaaa ccaggtacaa tcacacctca tacaaaattc tctgcagaag
     6901 tatacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcact aactaccgtc
     6961 cacaattcta cttccgtaca actgacgtaa caggtgttgt tgaattacca gaaggaactg
     7021 aaatggttat gcctggcgac aacgtaacaa tggaagttga attaatccac ccaatcgcta
     7081 tcgaaaacgg tactaaattc tcaatccgtg aaggcggacg tacagttggt gccggtgtcg
     7141 ttacagaaat caaagcttaa ttcacttaag ttttaaagta aagcaacatt tattcggacg
     7201 taatcgctcg gaacgaaggt cttaggatct ttgttccgag cttttttatt tattgcagga
     7261 tcaggaaaaa agacaggagt attataaata gaagaaacac gaaataaaag agtcagtatt
     7321 ttctaaatga aactttatga aaacaaaatg ttttttacaa gaaaaagcct tgctttgtga
     7381 gtttatatat attataatga caaaggtgcg tttactttag tgaattgcac agacgtaatc
     7441 agaatgattt cgtcggctaa gaaacgagag gttgcgacac gcccggacgc tttgccacga
     7501 acgagcgtga cggaaatttt cgtggagcta tgtctacttt tcaaaatagg cgaaggaggg
     7561 aacaagatgg caaaacaaaa aattcgtatc cgtttaaaag cgtatgaaca ccgtatttta
     7621 gatcaatcag cggataaaat cgtggaaact gcaaaaagaa ctggagctag cgtgtctggt
     7681 ccaattccat taccaactga acgcagtctt tacacaatca tccgtgcgac tcataaatac
     7741 aaagattcac gcgaacaatt cgaaatgcgt acacacaaac gtcttatcga cattgtgaac
     7801 ccaacaccaa aaactgttga tgctttgatg aagctagact taccatctgg tgtaaacatc
     7861 gaaatcaaat tataaaacta aaacaaaaat aatggaggtg tactcatgac caaaggaatc
     7921 ttagggaaaa aagtgggaat gacacaaatc ttcactgaat ctggtgaatt aattccagta
     7981 actgttgtag aagctactcc aaacgtagta ctacaagtga aaacaatgga aacggatggc
     8041 tacgaagcta ttcaagttgg ttaccaagat atgcgtgaag ttttatcaaa caaacctgcg
     8101 aaaggtcatg ttgcaaaagc aaacacggct cctaagcgct tcattcgtga attcaaaaat
     8161 gttgagctag gagaatatga agtaggaaaa gaaattacag tagatgtttt ccaagcagga
     8221 gacattattg atgtaacagg tactacgaaa ggtaaaggat tccaaggggt tatcaaacgc
     8281 cacggacaaa gccgcggacc aatggctcac ggttctcgtt accatcgtcg tcctgggtca
     8341 atgggtccag ttgcacctaa ccgtgtattt aaaaacaaac gtcttgctgg ccgtatgggt
     8401 ggcaaccgcg taactatcca aaacttggaa gttgtacgtg tcgatgcaga aagaaatgtt
     8461 atcttgatca aaggtaatgt tcctggagct aaaaaatctt taattactat caaatcagct
     8521 gtgaaagcta aataattgta ggaagaaagg aggaactaag gaatgccgaa tgtagcatta
     8581 ttcaaacaag atggaagcca aaacggtgaa atcacactta acgaagaaat cttcggaatt
     8641 gaacctaatg aaagtgttgt ttacgatgca atcgtaatgc aacgtgcgtc attaagacaa
     8701 ggaacacacg cggttaaaaa ccgtagcgct gttcgcggcg gcggacgtaa accatggcgt
     8761 caaaaaggaa caggtcgtgc acgtcaagga tctatccgct caccacaatg gcgtggaggt
     8821 ggcgtagtct ttggaccaac accacgttct tacagctaca aacttcctaa aaaagttcgt
     8881 cgtttagcaa tgaaatctgt attgtcagaa aaagttgctg aaaacaactt ggtagcaatc
     8941 gaaggcttga acttcgacgc accaaaaaca aaagaattca aacaagttct tgctaactta
     9001 tctatcgact ctaaagtatt ggttgttcta gaaaaaggta acgactttgc tgctttatca
     9061 gcacgtaact taccaaacgt ttctgtagta acttctgata atgtgagtgt gctagacgtt
     9121 gtatcaaata caaaagtgtt agcaacacaa actgctctta ctcaaattga ggaggtgctt
     9181 gcataatgaa cttactagac gtaatcaaac gcccagtgat cactgaaaaa tcaatgcttg
     9241 ccatggatga caagaaatac acttttgaag tggacactcg cgcaaacaaa actttagtaa
     9301 aacaagcggt agaagctgct tttgacgtta aagtcaaaaa cgtgaacatc gtgaacgtac
     9361 gtgcgaaatt caaacgtatg ggcaaatatg caggatatac aaaaaaacgt cgcaaagcga
     9421 tcgttacatt gactgaagat tcaaaagaaa tccaattatt cgaagctgct gaataagcac
     9481 taatagataa ctcattaaat taggagggaa aaacgtggcg attaaaaagt acaaacctac
     9541 ctcaaatggt cgtcgtaaca tgacaggttc tgattttgct gaaatcacaa catcaacacc
     9601 tgaaaaatca ttgttacaac cattgaaaaa ccatgctgga cgtaacaaca acggccgcat
     9661 cactgtacgt catcaaggtg gcggacacaa acgccaatat cgcgtgatcg acttcaaacg
     9721 taacaaagat aacgttgttg caactgttca aacgatcgaa tatgatccaa accgttcagc
     9781 taacatcgcg ttagtacact atgaagatgg agtgaaagct tacatcttag cacctaaagg
     9841 actagaagtg ggtatgaaac ttgtttcagg tccagaagca gatatcaaag tcggaaatgc
     9901 tcttccatta gaaaacattc cagttggtac tgttatccac aacattgaaa tgaaacctgg
     9961 taaaggtgga caattgatcc gttctgctgg tacaagtgct caagtacttg gtaaagaagg
    10021 caaatacgta ttgatccgct tgaactctgg tgaagttcgt atgatcttag ctgcatgccg
    10081 tgctacaatc ggttctgtag gtaacgaaca acacgaatta atcaacatcg gtaaagctgg
    10141 acgctctcgt tggatgcgta aacgcccaac tgtacgtggt agcgtaatga accctaatga
    10201 tcacccacac ggtggtggtg aaggtaaagc tcctatcgga cgtaaagcac cagtttctcc
    10261 ttggggtcaa ccagctcttg gttacaaaac tcgtaacaaa aaagctaaat cagacaaact
    10321 tattgttcgt cgtcgcaaaa ataaataatt actttgacca tgtgtcgcac attttgagag
    10381 gaggttcacc atgggtcgta gtttaaagaa aggacctttt gtcgatgagc atttaatgaa
    10441 aaaagttgaa gctcaagcag gggttgaaaa gaaaaaagtt atcaaaactt ggtcacgccg
    10501 ttcaacagtt ttcccaagtt ttgttggata taccatcgca gtttatgatg gacgtaaaca
    10561 tgtaccagtt tacatccaag aagacatggt aggacacaaa ttaggtgaat ttgcacctac
    10621 aagaacttat cgtggacatg ctgctgacga taagaaaacg aaacgctaat ttgaggggag
    10681 gataaatcca aatggcagaa caaatcacat cagctaaagc aactgcaaaa acagttcgca
    10741 cttcacctcg aaaagcccgt ttagtaattg accttatcag aggtaaaagt gtcgcagatg
    10801 caatttcaat cttgaaattc acaccaaaca aatctgctgg aatcattgaa aaagtattga
    10861 tgtcagcagt tgcgaatgca gaaaataact ttgacttaga tgttgaaaac ttggtagtaa
    10921 gcgaagcttt tgttaacgaa ggacctacaa tgaaacgttt ccgtcctcgt gcaaaaggat
    10981 cagcttctcc aatcaacaaa cgtacaagtc atatcacggt agtcgtatca gaaaaataag
    11041 gagggataat cagtgggtca aaaagtacat ccaattggaa tgcgtgtagg catcatccgc
    11101 gactgggatg ctaaatggta tgctgaaaaa gagtatgctg agttcttaca cgaagattta
    11161 agaatccgta aatttatttc aactaaactt gctgatgctg ctgtatcaac agttgaaatc
    11221 gaacgcgcaa caggtcgcgt aaacatttca attcacacag ctaaaccagg tatggttatt
    11281 ggtaaaggcg gttctgaagt cgaaagccta agaaaagaat taaacaaatt aactggtaaa
    11341 agaatccata tcaatatcgt ggaaatcaaa aaaccagatc tagatgcaaa attagtaggt
    11401 gaaggaatcg cacgtcaatt agaaaaccgt gttgctttcc gtcgcgctca aaaacaagcc
    11461 atccaacgct caatgcgtgc tggcgctcaa gggatcaaaa ctcaagtatc tggtcgtcta
    11521 aacggagcag atatcgctcg ttcagaagga tactcagaag gaactgttcc tcttcatact
    11581 ttacgtgcgg acattgatta cgcatgggaa gaagcagata caacatacgg aaaactagga
    11641 gttaaagtgt ggatctatcg tggagaaatt cttccaacta aaaaaaacac tgagaaagga
    11701 gggaaataat catgttagta cctaaacgtg taaaacaccg tcgtgaattc cgcggaaaaa
    11761 tgcgcgggga agctaaaggc ggaaaagaag tagcattcgg tgaatacggt ttgcaagctg
    11821 ttgattcaca ttggatcaca aaccgccaaa tcgaagctgc tcgtatcgca atgactcgtt
    11881 acatgaaacg tggtgggaaa gtatggatta aaattttccc tcacaaatct tatactgcca
    11941 aagcaattgg ggtacgtatg ggttctggta aaggggcacc tgaaggatgg gttgcaccag
    12001 taaaacgtgg taaaatcatg tttgaaatcg caggcgttcc tgaagaagta gctcgtgaag
    12061 cgttacgtct agcttctcac aaattaccaa tgaaaactaa gatcgtaaaa cgtgaggaaa
    12121 tgggtggtga atcgaatgaa ggttaaagaa atcagagaat taaccactgc cgaaatgctt
    12181 gatcaagaaa aacaattaaa agaagaattg tttaatctta gattccaatt agcaacaggt
    12241 caattagaaa acactgcacg tattaaagaa gtacgtaaat cgattgcacg catcaaaact
    12301 gtgttgcgtg aacaagctaa gtaattatgg aaggaggcca ttaagcgtat gactgaagga
    12361 agaaatcaac gcaaagttta tcaaggtcgc gtggtttcag acaaaatgga taaaacaatt
    12421 accgttgctg tcgaaacaaa gaaaaaccac cctatctatg gcaaacgcat gaactattct
    12481 aagaaataca aagtgcatga tgaaaacaac acagcaaaag ttggcgacat cgtgagaatt
    12541 atggaaactc gtccattatc agctacaaaa cgtttccgtt tgttggaagt agtcgaagaa
    12601 gcagttatta tctaataaca cgagattttc ctatatagat tgaatgcaaa atctgaaagg
    12661 aggatacaca acgtgatcca agcagaaagt cgtttaaaag tcgctgataa ctcaggcgca
    12721 cgtgaaattt tgacgatcaa agtccttggt ggatctggtc gtaaaactgc taacatcggt
    12781 gatgtgatcg ttgctacggt taaacaagca acgccaggtg gggttgtcaa aaaaggtgac
    12841 gtcgttaaag ccgttatcgt tcgaacaaaa tcaggagctc gtcgtacaga cggttcatat
    12901 attaaatttg atgaaaatgc tgcagtgatt attcgtgatg ataaaagccc tcgtggaaca
    12961 cgtatctttg ggccagttgc acgtgaacta cgtgaaaaca acttcatgaa gatcgtttca
    13021 ctagcaccag aagttttata atcttgagac acttatcaaa ggaggtgcga aacagttatg
    13081 tttgttaaaa aaggcgataa agttaaagtt atcactggaa aagacaaaaa caaagaaggc
    13141 gttgtactag cagcgttccc gaaaaaagat aaagttattg tcgaaggtgt aaatgtcgtt
    13201 aaaaaacacc aaaaaccttc tcaagctgct cctcaaggtg gaattgttga gatggaagcg
    13261 ccaattcatg tttctaacgt gatggtggtt gactctactg gtgtagctgg ccgagttggc
    13321 tacaaagaag tagacggcaa aaaagtccgt gtttctaaaa aaaccggtga agttttagat
    13381 aaataaaagt ttaaggaagg agggtcttac tgaatgaacc gcctaaaaga aaaatatctt
    13441 aaagaagtaa ctccatcatt gatggaaaaa tttaactata gctctgttat gcaaacacct
    13501 aaagttgaaa aaatcgtcat caacatgggt gttggtgatg cggtatcaaa cgctaaaaac
    13561 ttagataaag cagttgaaga attaactttg atcactggtc aaaaaccaat gattacaaaa
    13621 gctaaaaaat caatcgctgg attccgttta cgtgaaggaa tgccaattgg tgcaaaagtt
    13681 acattgcgcg gagaaagaat gtacgaattc ttagacaaat tggtttctgt ttcactacca
    13741 cgtgtacgtg acttccatgg tgtaagtaaa aaagcctttg acggtcgtgg aaactatact
    13801 ttaggtatta aagaacaatt gatcttccca gaagtagatt atgatttagt agataaagta
    13861 cgtggtatgg acatcgttat tgtaacaaca gcaaactctg atgaagaatc tcgtgagttg
    13921 ttgacacaat taggcatgcc attccaaaaa taattaaagg aggcgaacta cgtggctaaa
    13981 aaatcaatga ttgctaaaaa caaacgtcct gcaaaatttt caacacaagc ttatactcgt
    14041 tgcgaacgtt gcggacgtcc acattcagtt tatcgtaagt tccatctttg ccgtatttgc
    14101 ttccgcgaac ttgcctataa aggtcaaatt cccggcgtga agaaagctag ctggtaaaaa
    14161 gtaaattcgc ataagaagga ggtaaaagat caatggtcat gacagatcca attgcagatt
    14221 ttctaactcg tatccgtaac gcaaacatgg ttaaacatga gtctttagaa gtacctgcat
    14281 caaaaattaa acgtgacatc gctgaaatct tgaaacgtga aggtttcgtt cgtgatgttg
    14341 aatacatcga agatgacaaa caaggcgtga tccgtgtttt cctaaaattt ggtaaaaacg
    14401 gcgaacgtgt tatcacaaac ttaaaacgta tctctaaacc aggtttgcgt gcttatgtca
    14461 aagctgacga agtaccaaaa gtattgaacg gtttaggtat cgcaatcatt tcgacttctg
    14521 aaggtgttgt cactgataaa gaagctcgtg aaaaaaatat cggcggcgaa gttatcgctt
    14581 acgtatggta atcaagtaga atctacaagg aggtgtcttt aagtgagccg tatcggaaat
    14641 aaagtcgttg ttatccctga aggagtaaca gttactcaag acggaaacaa cgttacagtt
    14701 aaaggaccaa aaggtgaatt gactcgtact ttttcagcag atatcaaaat gaatatcgaa
    14761 ggaaacgaag taactttcac tcgtccaaac gacagtaaag aaatgaaaac aattcatgga
    14821 acaactcgtg cgaacttcaa caacatggtt gtaggtgtca gcgaaggttt ccaaaaagcg
    14881 ttagaactaa tcggggttgg gtaccgtgca caattacaag gatcaaaact tgtattgaac
    14941 gttggttact cacatccagt agaaatcaca ccaccagctg gagtaactgt tgaagtacca
    15001 gctaacacac aagtaattgt taaaggtgcg aacaaagaag aagttggcga attagctgca
    15061 aacattcgtg gaactcgtcc tccagaacct tataaaggta aaggtattcg ttatgttggc
    15121 gaatatgtac gccgtaaaga aggtaaaact ggtaaataat cgtttagaaa aattgcatag
    15181 aagccagcag caggaaaaag gcatttggct gaagtttacc ttcaaaaaaa tacattttta
    15241 cttgtgccgc agcttctatt tttcgaaacg tatttactgc ttgaaatagt agtacaataa
    15301 aaataaagag gtgacaattg tgattacaaa accagataag aacaaaacac gtcaaaagag
    15361 acatcgtcgt gtacgtaaca atatctctgg tactgctgag cgcccacgct tgaacgtttt
    15421 tcgttctaac aaaaacatct acgctcaaat tattgatgac gtagcgggtg taacgctagc
    15481 aagtgcctct accttggata aagaaatttc aggtggaaca aaaacagaag cagcacaagc
    15541 tgtcggtaaa ctagttgctg aacgtgcagc tgaaaaaggc attaaagtag tagtctttga
    15601 ccgtggtgga tacctttacc atggccgtgt gcaagcttta gctgaagctg ctcgcgaaaa
    15661 tggactagaa ttttagaaga aggaggaata ccattcatgg tttatatcga tccaaaacat
    15721 ttggaattag aagaccgcgt tgttgcaatt aaccgtgtaa caaaagttgt taaaggtgga
    15781 cgtcgtctac gttttgctgc tttagttgtt gtcggtgaca aaaacggaca cgtagggttt
    15841 ggtacaggta aagctcaaga agttcctgaa gcaatccgta aagcaattga agatgcgaag
    15901 aaaaacttag ttgaagtacc tatggttggt tcaacaatcc cacacgaagt aatcggtgct
    15961 tttagtggtg gtagaatcct aatgaaacct gctgtagaag gttctggggt tgctgctggt
    16021 ggaccagttc gtgccgtatt agaattagct ggggtagcag atatcacttc taaatcttta
    16081 ggctcaaata caccaatcaa cgttgttcgc gcaacagttg aaggtttgaa acaattgaaa
    16141 cgtgcagaag aagttgcaga acttcgcggc aaatcagttg aagaattaat cggataagga
    16201 ggacaacaat aatggctgaa ttaaaagtta ctttaaaacg cagtattatc ggacgtcctc
    16261 aaaaccaacg cgatacagtt aaagcgttag gtcttacaaa gattaacagt tcagtggtta
    16321 agccttctaa tgaagcaatt aaaggcatga tcaatactgt ttctcaccta gtggacgttg
    16381 aagaaattta atttagaaaa atcataacta gaacattaag gaggtgccaa acctatgaaa
    16441 cttcatgaat taaaacctgc tgaaggttca cgccaagtac gtaaccgtgt tggtcgtggt
    16501 acttcatctg gtaacggaaa aactgccgga cgcggacaaa aaggacaaaa agctcgttca
    16561 ggcggcggtg tgcgtttagg atttgaaggg ggacaaacac cattgttccg tcgtttacca
    16621 aaacgaggat tcacaaacgt gaaccgtaaa gactatgcag tcattaatct tgatgtattg
    16681 aaccgctttg aagacggagc tgaagtaact cctgttgctt tagttgaagc tggaatcgtg
    16741 aaaaacgaaa aagctggaat caaagtatta gctaatgggg aactaaacaa gaaattaact
    16801 gtgaaagcag caaaattctc taaagcagct caagaagcaa ttgaagcggc tggtggatca
    16861 atcgaggtga tctaatgttt agactattaa aagatgcttt taaagtaaaa gacattagat
    16921 cgaaaatcct atttactgta tttgtgttgt ttgtatttcg cctaggtgca catattactg
    16981 taccaggcgt aaatgctgct tcattgcaaa acctttcttc tttgccattt ttcaacatgt
    17041 tgaacttggt aagtggtagt gcgatgcaaa acttttccat tttctcaatg ggggtttcac
    17101 catacattac tgcttctatc gtcattcagc ttcttcaaat ggacatcgtg cctcgatttg
    17161 ttgaatggtc gaaacaaggg gaagttggtc gtaaaaaact gaatcaagcg actcgatatc
    17221 tgacattagt attggcattt atccaatctg tcggtatcac tgctggattc aatcaatacg
    17281 ctcaattagg tttcgtgaat aatcctaacg tagcaactta cgtaacgatc ggtttgattc
    17341 taacagccgg tacgatgttt gttacttggc taggagaaca gatcacagaa aaaggtatcg
    17401 gaaatggcgt atcaatgatc atttttgcag gtatcatttc tagattgcct gaaagtatca
    17461 gagaaattta tgaagattac tttataaata ttgatccttc agacatatgg aaatctgcta
    17521 tttttgtggc cattttacta gtagcggtct tgttgatcgt gacattcgta acatttttcc
    17581 aacaagctga acgcaagatc ccgatccaat atacgaaacg cgtagctggc gcgcctacaa
    17641 gcagctatct gccgttgaaa gtaaatgcag ctggagttat tccagttatc tttgctagtt
    17701 cgtttattgc tacgccaaat gctgttttac aagcattgag cggtagattc ggttctgaaa
    17761 actggtacaa agtgatgatg gagatcttta actataatac agcacctggt gctacgattt
    17821 atacgattct gatcgttgcc ttcactttct tctacgcctt tgttcaagta aaccctgaga
    17881 aattagcgga aaacttgcaa aagcaaggaa gttatatacc aagcgtacgc cctggtaagg
    17941 ggactgaaga gtatgtctca agattactga tgcgtttaag tacagttggt gcagtcttcc
    18001 ttggtttagt ggcattgctt ccaatcattg ctcaaatggt ttgggatctt ccacaatcaa
    18061 tcggattagg cggtacaagc ttactgatcg ttatcggtgt agctttagaa actgcgaaac
    18121 agttagaagg cttaatgctg aaacgtaaat atgttggatt cattgagtaa gaaagcgtgt
    18181 tgaggtttcc cctcaacaca cgttctcaca catttattag gagggaaaac aatgaacctc
    18241 attttaatgg ggctgccagg cgcaggtaaa ggaacacaag ctgaaaaaat cattgatgct
    18301 tatggtattc cgcacatctc aacaggagat atgttccgcg cagcgatgca aaacgaaaca
    18361 gctcttggcc tggaagcaaa gtcttatatg gataaaggtg cattagttcc tgatgaagta
    18421 acaaatggaa tcgtgaaaga gcgactagct gaaccagata cagagaaagg cttcttatta
    18481 gatggctttc cacgtacgct agatcaagca aaagctctgg acgcaatgct aaaagatttg
    18541 aataaaaaaa ttgatgctgt catcgatatc catgttggtg aagaaatctt ggtcgaacgt
    18601 ctagccggcc gatttatctg cagcaattgt ggagcaactt accataaaat tttcaaccct
    18661 acaaaagttg aagacacatg tgatcgttgc ggtgggcatg aattctatca aagagaagat
    18721 gataaacctg agacggttaa aaatcgtctg gctatcaaca tcaaaaatag tgaaccaatc
    18781 ttggcttatt ataaagaaca aggtttgcta aacactattg atggtgctcg tgaaatcgat
    18841 gctgtatttg cagatgtaca aaaaatcatt gaaaaatagc acgattgccc gtagccgact
    18901 tgcattatgc agttactctt gtcaagttca ataaattatg atagaatatg tcagtccgac
    18961 ttctagagaa tcctctagat gatccatttt gagaattttg aggtgggaac tgcagtttcc
    19021 gccattttat cgtgtgaaaa tgcgaaacaa gtacaaggag gtactgtgcg tggcaaaaga
    19081 agatatgatt gaagtcgaag gtacagtcgt cgaaactttg ccgaatgcaa tgtttaaagt
    19141 tgaactagaa aacggacacc aagttcttgc tactgtttca ggtaaaattc gtatgcatta
    19201 catccgtatc ttacctggtg acaaagtaac ggttgaattg tctccatacg atcttaaccg
    19261 tggccgtatt acttatcgct ttaaataatt gtactccgta aaactacagg aggtattatc
    19321 atgaaagtaa gaccatcagt aaaaccaatg tgcgaacatt gtaaagtaat tcgtcgtaaa
    19381 ggacgcgtta tggtgatttg cccagcaaat ccaaaacata aacaacgtca aggataattg
    19441 gaggtgctat aaatatggct cgtattgcag gagtagatat tcctcgtgat aaacgtgtag
    19501 tagtttcact tacttatatc tatggtattg gtaacactac tgctaaaaag atcttagctg
    19561 atgctggtgt atctgaagat gttcgcgttc gtgaattaac gaatgaacaa acagatgcta
    19621 tccgtgcaga agtagataaa ttaaaagttg aaggggatct tcgtcgtgaa gtgaacttga
    19681 acatcaaacg tttgatggaa atcggttctt accgtggaat ccgtcatcgc cgtggattgc
    19741 ctgttcgtgg acaaaacacg aaaaacaatg cacgtactcg taaaggccca gctcgcgcta
    19801 tcgctggcaa gaaaaaataa ttcttaagtg aaggaggtta aaacttcatg gcagcaaaaa
    19861 aagtgaatcg taaacgccgt gtgaagaaga atatagaagc aggtattgca catatccatt
    19921 ctacattcaa taacactatc atcatgatca ctgatactca cggaaatgca ttagcgtggt
    19981 catcagctgg ttcattaggt ttcaaaggaa gcaaaaaatc aactcctttt gccgctcaaa
    20041 tggcagcaga aactgctaca aaagcagcaa tggaacacgg attaaagact gttgaagtaa
    20101 cagttaaagg acctggttct ggacgtgaag cagcaattcg ttcattacaa gctacaggtt
    20161 tagaagtgac tgcaattcgt gacgtgactc cagttcctca taatggtgcc cgccctccaa
    20221 aacgccgtcg tgtttaatga gtgccgctca ttttgagttt tatcagtaac gtcattgaac
    20281 aagacacttt tcgttttgaa aggggtaaag ataagaatga ttgagttcga aaaaccaaga
    20341 atcgcaaaaa ttgatgaaga aaaagattat ggcaagttca tcgttgaacc tcttgaaaga
    20401 gggtatggga ctactttagg gaattcccta cgtcgtattt tattatcttc tttgccaggt
    20461 gctgccatca ctaatattca aatcgatggt gtactgcatg aattctctac catcaaaggt
    20521 gttagagaag acgtcactca aatcatcttg aacattaaag gcttagcgct gaaaatgtat
    20581 ggccaagaag aaaaaaccct tgaaatcgat attaccggac ctgctacagt aactgctggc
    20641 gatattatcg ttgatagtga tgtagaaatt cttaacaaag atatgtacat ctgtagtgtc
    20701 tctgaaggag ctacgttcca tgctcgctta acagtgaaac ctggacgtgg ttatgttcaa
    20761 gcagatgaaa ataaaaaaga agatatgcca attggcgttc ttccagtcga ttcaatctac
    20821 acacctgttc gtcgtgtgaa ctatcaagtt gagaacacac gtgttggaca ccgtgaagat
    20881 tttgataaat tgacgatgga gatttggaca gatggttcaa ttgaaccgtt ggaagcaatg
    20941 agtttgtctg cgaaaatcat gactgaacat ttggatattt ttgtgaactt aactgatgaa
    21001 gcaaaaaatg ctgaaatcat ggtcgaaaaa gaagaaacac aaaaagaaaa aatgttggaa
    21061 atgacgatcg aagagttaga cttgtctgtt cgctcttaca attgtttgaa acgtgcagga
    21121 atcaatactg tacaagaact tacaaataaa tctgagcctg aaatgatcaa agtccgtaac
    21181 ttaggacgta aatcattaga agaagttaaa gcaaaattgc acgacttagg tttaggttta
    21241 cgtaaagaag attaaaacct tttacgaagg agggaaacca acgtgagtta tcgtaaacta
    21301 ggacgcacat ctagccaacg taaagcgatg ttgcgtgatt taacaactga tttattaatc
    21361 aacgaacgta ttgtgactac tgaagctcgt gcgaaagaag ttcgctcaac tgcagaaaaa
    21421 atgatcactt taggtaaacg cggagatttg catgcacgtc gtcaagctgc ggctttcgta
    21481 cgtaatgaag ttgccagcgt gcgtgaagaa aatgaggaca tcgtcattga atcagcttta
    21541 caaaaactgt tcaacgatat tgcacctcgt tacgcagaac gtcaaggcgg atacacacgc
    21601 attttgaaga cagaaccaag acgcggtgat ggtgcaccaa tggttgtctt agaacttgtc
    21661 taaaatcaca ctaataacat cactttatag atgattcttt tagagtgtta tgatgttgga
    21721 agatattttt ccgagtctag ctcagcgctg ctcctcaagc gaatatcgct tggggagtaa
    21781 gcactcatca taaagtgttt ttttatacct tcaattacct gtcatgacag atatgtttga
    21841 aggcataaaa aaacatttta agcgagaaat aattgaaaat tttgtctttt tttgatagtg
    21901 ttagtaggaa ctttaggatg aagaaggagt aatcatggag ccaattattg aattagaaaa
    21961 gataaattat aaataccagc cagatgatct tcgccccgca ttgaaagatg tctcctttac
    22021 aattgataaa ggtgagtgga tcgctattat tggacacaat ggttcaggaa aatcgacact
    22081 tgccaaaacg atcaatggat tgcttctgcc agaatcaggg atcgtgaaag tgggaaatca
    22141 aatattggat gaagaaaata tttggacgat ccgacagatg gttggaatga tttttcaaaa
    22201 tccagacaat cagtttgttg gttcgacagt agaagatgac gttgcttttg gtcttgaaaa
    22261 tcaagggatt cccagagaag aaatgctagt gcgtgtgaaa gatgcacttg agaaagttag
    22321 aatggctgag tttgcttcac gcgagcctgc tcgtttatca ggagggcaaa aacaacgtgt
    22381 tgccattgcc ggcgtggttg ccttacgacc tgatatcatt attttagatg aagcaaccag
    22441 tatgctagat ccagaaggtc gtgaagaagt gatctctacg atcaagaaga tcaaagaaga
    22501 aagtcaattg accgttatat ctatcactca tgatattgat gaagcagcta atgccaatcg
    22561 gatactagtg atgagacaag gcgaacttgt ccgtgaaggt acaccaaaag aaattttttc
    22621 tgctggtcct gaactgatcg acttaggtct agatctacca tttcctgaaa aactgaagag
    22681 cgcactgaaa gaacgtgggg ttgatgtgcc cagtgaatat atgacagaag aaaggatggt
    22741 ggattggtta tggacatccg ttttgaacaa gtagacttca cttatcagcc gaatacacca
    22801 tttgaacaaa gagccttatt tgacatcaat atgacgatca aagaaaacag ttatactgca
    22861 ttagtagggc ataccggaag cgggaaatca accttacttc agcatttgaa tgcgcttgtt
    22921 aagccaacaa gcggtacagt ccatattgga gaacgagata ttcagccgga tacggataat
    22981 aagaatttaa agcccatccg aaaaaaagtt ggcatcgtct ttcagtttcc agaagcacag
    23041 ctattcgaag aaacggtagc aaaagacatt gctttcggtc ctaaaaactt tggtgtcagc
    23101 gaagaagaag cactagtcct agcaaaagaa acattagaac aagttgggct ggatgaaagc
    23161 tatttggaac gttcgccatt tgaactttca ggagggcaaa tgcgtagagt agcaatcgct
    23221 ggtgtgcttg ccatgaggcc ggaagtactt gtattggatg aaccaacggc aggacttgat
    23281 ccccagggca gaaaagaaat gatggagatg ttttggcgtc ttcataagga acagcagatc
    23341 actattgtat tagttacgca tttaatggat gatgtagcca attttgcaga ttatgtctat
    23401 gtgttggaaa agggacgaat cgttaatagc ggggagccgc aagaggtctt tcaaaacatt
    23461 gaatggctaa aagaaaaaca actaggcgta cctactgcca ccgagtttgc agaagaatta
    23521 atggctaaag ggatgaactt tgctaccttg cctttgacag ctgaagaatt agcagacgct
    23581 atcgtccatt atgctggagg aaatgcccat gatgaataaa ttgatatttg gtcgttatat
    23641 gcctggagat tccttgatcc atcgattgga tccccgagct aaattattag ccagttttta
    23701 ttttatcgga atcatttttt tagcaaataa ttgggaaacg taccttttgt tggcattttt
    23761 cacattactt tctgtcttct tgtcaaaagt agatttgaaa ttttttatcc gaggcattcg
    23821 cccattgatc tggctaatcc tcttcactgt agctttgcag gtcttgttta caagtggcgg
    23881 agaaacttat tggagttggg gtgttttcca tatcactgaa tttggtgtga tgaacggcct
    23941 ctttattttc tgtcgattcg tgttgatcat ttttatgtcg actctactca cgcttactac
    24001 accgcctttg gaattatcgg atgcgattga gtacttgctt cgtccattga aagccgtacg
    24061 gttcccagtc cacgaaattt cattgatgct gtcgattgct cttcgttttg tgcctacatt
    24121 gatggacgaa acagaaaaga tcatgaacgc tcagcgcgct cgcggtgttg attttggtga
    24181 aggaaaccta gttcagaaaa tgaaagcagt cattcctttg ctgattcctt tatttgtcag
    24241 cagcttcaac cgtgcggaag atcttgcgac tgctatggaa gcaagaggct acaaaggtgg
    24301 ggaaggaaga acgaaatatc gtgtattgca ttggcataat agagacacga tggttatcat
    24361 tgcttttgta ttattaactg tgctactgat ctttttaaga ggataaacgt tcagtgggcc
    24421 gctatctatt cttttcttgc tgatctgttt gcaagaaagg atgcaagcag tgtatgacaa
    24481 gaaatattag ggtctgtggc agactaaggt cacaggcctc tttcttttat ctgaaaagaa
    24541 agaacgaaaa aattttatag aaaaatcatt attccagata accttttagg aacaaaggag
    24601 agaaaaacat ggtacgttac aaagcaatca ttgcgtacga cggaacaaat tttaatggat
    24661 tccaaaaaca gccaaatggg cgtactgttc aagaagaagt agaaaaaaca ttacaaaaaa
    24721 tggcaaatgg aaaagaaatc acggtctttg gttcgggaag gacagatgcc ggagtacatg
    24781 cgatcgggca agtaatccat tttgattatc cagaggagcg tccgttagaa cgaatgcgtt
    24841 tcgcgctaga tacccagtca ccagaggata ttgctgtaag acaagtagaa attgtttcag
    24901 aagacttcca cgcaagatat ttggtcaaag aaaagacgta tcaatttcga gtggatatag
    24961 ggaaaccacg cagtcctttt cgacgccatt atgcaagtta ttttccttac ccacttgatt
    25021 tggaaaaaat tcagcgggcg cttcctgatt tattagggac tcacgatttc acctcttttt
    25081 gtgcatcagg aagctcgata gaggataaaa tacgtacgat ctacgaggct aagatggagg
    25141 taaatgaaac aaaagacgaa ttgttgttta catttagggg aaatggcttc ttgtataaga
    25201 tgatacggat cttagtagga acgcttttga aaattggcaa tggccgattg cctgaagata
    25261 gtatcccaga tattattgcg aaaaaagatc gtaatgcagc aggacctaca gcacaccctg
    25321 aaggacttta tttgtatgaa gtgaaatatg aataagaggt tatgatagaa gtggtcagct
    25381 tcaagaaata agaaggtatt tacgaaaatt acttctcaaa attttgcaaa atttcggctt
    25441 attttcgaag aagctacttc tggaacaccg tttaagaaag aggtcgtgag cctgtcattt
    25501 aactctgagt cacaaagagc aatttcaaaa aacagcttct catctttgat gaatatcgtg
    25561 acttgtggac gaggcgttga taagtgaatc atctttttgt tacctatttt ttctgtaacc
    25621 aaaaaacgat ggaacgttgt tttgattgcc cttggtgcgg acagtctgaa caggttccat
    25681 cgtttcttta ttttctacgt cttcttctgt gtgtgtttcg tggttgttct tctattgctg
    25741 gatcaatcgt gccataaaag gggcgattgc ggtcaaggta ccagacataa agaataaaca
    25801 atgcaataca taggacaaaa aggattgcac ctatcgtaaa tccttcaggc aagtaactga
    25861 aagtgacagt atgtttacct gctggaactt ttatcatcaa gaatgcgtct ttgaaattgc
    25921 tgaccttgac tggtttccca tcaattttag ctttccatcc tttgtcataa ggaatagttg
    25981 tcaccaacat ggaatcttca gcggtattta cagtcccaga agcttttctg ccagtggctt
    26041 ccatttcgac acctttttct ttgatctgat tcattgcggc ttgataggct acaacatcta
    26101 gaccaacgac tttaggctcc atgaaactta tttctttcgt cccataaaaa cttgcagtaa
    26161 atgtcactgt tgtctcttga ggataatagc caagatcata gtattgacca gaaatgttga
    26221 tttgcgtttt tcgcgatgta ccattgacag tgatcgtcgc actagaactt tctaattgcg
    26281 caaaattggt tgggaacaaa cttaagtaag cttgtgtatt tgcaggcaca tggacttccc
    26341 aagtgagctc ttttggctgg ttatctgctg tttctgagta agtgacgcca gtcgttgtct
    26401 ttgtgatcgt cgtattcaac tgattcttca aagtaggtgt atagaatgta aaatactgtt
    26461 catttagacc agacaatgta ttgatcaagt ttgtctgact ggtcaaatta tctgtcactg
    26521 gctgctcaac atcattaacg gacaacggtg cagtaaatgc tagtggcaat gcattgacat
    26581 tctgatacag tgtatagtcg ccgctttgac cagcttggcg gaagccatat ttactgaacg
    26641 tgctttcgtt tttagaaatg ttatatttga ttcctgtaaa cccatccatc agcaaagtat
    26701 tattgttata acggatattc aaatttgtcc ctcttgaacg gaaacctaat ttatccaggt
    26761 aagaggaaga attacgattt cttatagaag aaaacaaact gataccactg taaccatagt
    26821 tgatcgaatc gtttgcagat actggatcca gattttccaa gcggtaaaac gagtcggaat
    26881 ctttttttgt ttcatttaca agagctttca agtcaggata aggatcagta tataaactgc
    26941 gggaagcata attccaatca tcaagaatgc catggatcat actgttcgta ttgaccagtg
    27001 cttcagcaga catgatcaat aataacaata tactcagatg attgaatgaa agttttttat
    27061 gctgataaaa tcccacagca aaagcataaa tccctaagaa aaccactgtc aatacaaagg
    27121 aagtcatcgt cacataggtg tagcttcctt caggtttgat gccccatgcc accgcaaaga
    27181 gagcagctac gaccaaaatg attcctaaca acagcccacc atcgtcttgt tctatctttt
    27241 cccatccttt tcctgcaagc aggagcacta agaaagagaa cagataggca taacggaata
    27301 aaaacatatt cggtgcatgc ataccatgcc agaacaaatt caatggaaca agataaaaac
    27361 ttgcgataag tatggcgaaa agactgccgt acaaaaattt atttttcaac ggtactttcc
    27421 gactggcaaa ataaaacaga cagaggacta atggtagcag acctgcgtaa acgaacggaa
    27481 tcgatccgta ttttgtcgtg tcataaacac caatcatatt tttcatgatt agatccagat
    27541 aagaagtcgc ttctgttttc aacgtagtga tctgactcag tgcctcgcca tttgaccgaa
    27601 gatccagcac tgctggcaat acaatgatca ttgaagcgcc gcctgccagt aaagaagtga
    27661 taccataagg aacaatcgtt tttttatatt ctgaccagtt tgtcaataga cgggcgataa
    27721 aatataagaa agaaaatacg ccaatcataa aacccatata aaaactggat aaaaacaaga
    27781 gtaaataact gataaataaa agtttcggcc gtttaagatc cataacacga tgaatcccca
    27841 aaatcactaa tggaagataa acaaatgcat ccagccacat gatgatctcc gaatgtgcag
    27901 tgataaatga gatcaatgcg taactgacgc taagtgcaac atgcccccag cgaggaatct
    27961 tgaacgtacg tgcaagaaac caaaaagcca atccggcaca gccgacctta agcaaagtca
    28021 gaacatacag tgcatctggc atcatctggt ttggaaaaaa gaacaccagt ggcgtgaaga
    28081 tcccgcctag atagtaagaa attaaagata aataattcaa tcctagagaa gcattccatg
    28141 tataaaaaat actttgcttt ccaagaagaa catttcggaa gctggcatga aaattagaaa
    28201 actgtgaaaa ggcatcactt gccaaaatac ttctgctgct tccaggatag attccgatac
    28261 ttagataaac aagtgctaaa atcaaaaacg gaatcaggaa actagctgct gcgtagcgtc
    28321 cgttttctct aaagaatgac tttaaatttt ttttcatatt tattttccac caaatttcta
    28381 tgaagttgta ttcagtttat catatccttg tttttctctg cttaaaaaga gtctagtttt
    28441 cacaaaaaaa taaaatttag aataaataac ttcataattt gttcgtataa caaaaataaa
    28501 cgcaaaaaaa gggtaggaat caaaagtccc acccttaaaa atttttatta aataaaaaac
    28561 cgttcactat ctgaacggtc caagtatcat tcaattaaac gcgttcaact tttcctgatt
    28621 tcaaagcacg agttgacacc caaacttttt taggttttcc gtcgaccata acgcgaactt
    28681 tttgtaagtt aggtttaaca gtacgttttg ttgagttcat agcgtgtgaa cggttgtttc
    28741 cactttttgt cttgcgacct gtaaagtaac aaacttttgc catggtaatt tcctcctttg
    28801 ccttgatttg gagcaataga ataattcagc tcataccaga ctaacatatc atatagaatg
    28861 ggaatttgca agtacatttt catagataca attgacaatt gagaagaata attgtaaatt
    28921 aaacaaggtt atacctgaaa ttttgcaagg tttttgagta ctttgggtaa ctgcctttta
    28981 tttcaagttg cgctatgata aaatatgata gaagaaaaat gggagttttt tacccgttaa
    29041 ggaggctcat tcatggctgt aaaaattcaa acgtcagctg gaaccattga aattaccaat
    29101 gatgtaattg caacagttgt tggtggcgcc gcgacagatg tgttcggtgt tgtaggaatg
    29161 gccagcaaaa atcaaattaa agataatatt actgagattt tgcgtaaaga aaattactca
    29221 agaggagtag ttgtccgcca agaagataat ggtattgctg tagatgttta tacgattatc
    29281 agctatggta cgaaaatctc tgaagtttcc cgcaacgtcc aagaaaaagt gaaatacaat
    29341 ctagaggcat tattaggcgt aactgccaat tcagttaatg tctttgtaca aggtgttcgt
    29401 gtactgccag actaggcagg agactggctg gtaactcagc agtcgatctg aaggaggatt
    29461 atctaggtga gtgtaacaga aataagtgca agccagttcc aggaaatggt ccaggcaggt
    29521 gcgaatcgtc tgcaagtaaa tgcagaatac gtcaattcat taaatgtatt ccctgtgcca
    29581 gatggcgata cgggaacaaa tatgaatcta tctatgacta gtggagctaa ggcagtagta
    29641 gattcaacct cagaaaaagt aggagaatta gcagcaatgc tttcaaaagg attgctgatg
    29701 ggtgcgcgcg gaaattccgg cgttattttg tctcaattgt ttcgtggatt ttctaagcaa
    29761 attccagatg ttgtctcatt aaatgcttct gatcttgctg cagccttcac tcatggagtt
    29821 gaaactgcgt ataaagcagt aatgaaacca gtagaaggaa cgattttgac agtagctaga
    29881 gaagcagcga aagctggaga aaagaaagca aaaagtactg atgatgtcgt tgaagtaatg
    29941 acagctgttg taaaaggagc aaagcgtgct ttagctaaaa ctccagactt gcttccagtc
    30001 ctaaaagaag tcggtgtcgt agacagtgga gggcaaggac tgctgtttgt ttatgagggc
    30061 tttttaagtg cattgaatgg agaattccaa gcggatgaaa catatgaacc ttctccagca
    30121 gaaatggatg atatggtcaa tgcagaacat catcgcagtg tccaaggtca aatggcgaca
    30181 gaagatatcc attttggtta ctgtacagaa atcatggtaa aaatcggaga aggacctaca
    30241 gtcgatagta cattcgatta cgaagaattc cgtaactatt tagatggtat aggagattct
    30301 ttacttgtgg tcaatgatga cgagatcatc aaagtccacg tgcatacaga aaacccagga
    30361 gaagtcttga attatggaca aaaattcggt tcattagtca aaatcaaagt cgacaacatg
    30421 cgcttgcagc atgagacgat tttggaacac gatgaagaag tgaacgagtt tgcgaaaaaa
    30481 gaaataccac gtatcccgta tggtatcgta gcgatcgctg ctggtaaagg cgtccaagag
    30541 ctgtttgaaa gtttaggcgc taattatgtt atcagtggtg gacaaacaat gaatccaagt
    30601 acggaagaca ttgttaaagc gattcaagaa gtaaacgcag ataaagtgat tgtcttgcca
    30661 aacaataaga acatcttcat ggcagctgat caagcagcag aagtagcaga tatcccagta
    30721 gcagttgttc cttcaagaac gatttcccaa ggattgacag ctatgctttc attcaatgat
    30781 caagcttctt tagaagaaaa caaagaaacg atgacagaaa tgttgtctag cgttgtgagc
    30841 ggtcaagtca ctcacgcgat cagagatacc tcaattgacg gtgtagagat caaagaagga
    30901 gactttttgg gcatgatcga tggaaaaatc ctgatctcta acccagatat cacagcaaca
    30961 gcgttaagta cactagaaca aatgatcaat gaggacacag agatcgtaac gatcttggca
    31021 ggtgatgaag gaacagttga acaagcaaat gaatttgctg agaaactaac agaaaaacat
    31081 cctgatttgg aaatcgagat ccaccaagga gatcagcctg tctatcctta tctattttct
    31141 gctgaataat ttctgtgtca gaatatcttt ctaacacagt attcattcat aaaaggcctt
    31201 cggttagtga tcaggtatga tcgctgacca agtccttttt ttgttaaagg agggaggaaa
    31261 aatttatgcg ttctttagat gatccaatca ctatgctggc aggagtcgga gaaaaaagag
    31321 cagctagtct tgccagcttg ggcatccaaa cgattgaaga cttactgact tattatcctt
    31381 ttcgctacga agatattcag gaaagaaatc taaacgagat ccaggatcaa gaaaaggtta
    31441 ctttgaaagg aatcgtagtc tcgcctcctg tgatgaatcg atttggttat aaaaaaagcc
    31501 gattgcaatt tcgtatgatg caagaacatg atgtgttcaa cgtttctttt tttaatcaac
    31561 cttatctaaa agaaaaagtc attttatcgg aggaaatcgc cgtatacggt aaatgggatg
    31621 caaagcgtaa agccttgaac gggatgaaga ttttaggttc tcaaacaatg gatgactttt
    31681 cgccgatata tcacgtcaac aaatcaattc gtcaaacgac attagttgat ctaatcaaaa
    31741 aagggtttca agaatacggc acattgattg aagaaaatct gccaaacaac cttgtggaga
    31801 aatatcgact gcttgaccga gcttctgctg taagaagtat gcattttcca aaagaccacg
    31861 aagaaaacca tcaagccaaa cgacgtgtcg tttttgaaga gttcttttta tttcaaatgc
    31921 gtcttcaagg attgaaaaaa gcagaaaaag cagaaacgaa tggtttggag atcctttatg
    31981 atgtccaaaa attgaaacaa tttactcaaa agctaccttt tgaactgaca aatgcacaaa
    32041 aacgtgtaac gaatgaaatc tgtcgggatc ttcgcagtcc gcagcatatg cagcgacttt
    32101 tgcagggaga tgtaggtagc ggaaaaacag tggttgccgc gattgcgtta tacgctaccg
    32161 ttacagcagg tttccaaggt gcgttgatgg tgcctacaga aatcttggca caacaacata
    32221 tggaaagctt gcaacagctg tttgatccca atgaagtaac catcgcattg attacggggt
    32281 caacgaaagc aaaagaacga cgtgagctcc ttgaaaaact agagcaaggg gaaattaacg
    32341 tcgtgatcgg cacacatgca ttgatccaag atggagtcga atttcagaat ttaggtctag
    32401 ttattacgga tgagcagcac cgttttggcg tgaaccagcg gaaagtactt cgtgaaaaag
    32461 gctggcgacc agatgtctta tttatgacag cgactccgat tcctagaaca ttagctatta
    32521 cagcatatgg tgagatggat gtatctatca ttgatgaact tccagctgga cgtatcccga
    32581 tcgaaacacg ctgggtaaga acacctcaat tggacagtgt actggattgg acgaaaaaag
    32641 aactggcaag aggacatcaa atgtatgtca tctgtccgct gattgaagaa tcagaagcat
    32701 tagatgtaaa aaatgcagtg gaaatctatg aaaaacttcg tgaactattt gcaccaaaat
    32761 atgaagttgg cttgttacat ggtaaaatga agaatcagga aaaagaagta atcatggaag
    32821 ccttcaaaga caaccagatg cagatattag tttcgactac tgtaattgaa gtaggggtaa
    32881 acgtgccgaa cgcgaccgtc atgctgatca tggatgcgga tcgatttgga ttggcacagc
    32941 tgcaccaact tcgtggacgt gttggacgcg gaagtgatgc ttcctactgt atcttagttg
    33001 ctaatcctaa aaatgaacta ggtgtggaac ggatgaaaat catgacagag accaccaatg
    33061 gttttgtact aagcgaaaaa gatctggaat tacgtggtcc tggtgaagta tttggcttcc
    33121 gtcaatctgg tctgcctcaa tttgcgattg ctgacttggt gacggatgga aatgttttgg
    33181 aggtagccag agaagaggct gcagcccttt ggcaaataaa ggattggcag atgcttcctg
    33241 agtatcagcc tttagcgcaa agcctccaaa tagaaaatca ggataatcgt tatttcgatt
    33301 agcagactgt gctatacttt atactgatga cggaaaaagg agtggaaaca atgaaaattg
    33361 ccgtagatgc aatgggcgga gatcacgcac ctcaagcaat tgtagaaggc gtggctttag
    33421 cgcagcaaga ttttccagaa gtagaattct tactttacgg aaaagaagca gaaatcaaga
    33481 aatacttaac aaatgaaaaa aacatcacga ttatccatac agacgaaaaa atcaatagtg
    33541 atgatgagcc agtaaaagca attcgacgaa aaaagactgc atcaatggtc cttgctgcac
    33601 aagcagtgaa aaacggtgaa gcagatgctg tattttcagc agggaataca ggcgcgctac
    33661 tagcagcagg gttgttcatc gtaggacgta tcaaaaatat cgaacgccct ggcttgatgt
    33721 ctaccttgcc agtagtaggg aaagaaggtg ccggtttcga tatgctggat cttggcgcta
    33781 acgcagaaaa caaaccagaa catttgttgc aatacggtat tttaggttct ttttatgcac
    33841 gtaaagtccg aggtatcgga cgtcctcgtg tcgcactctt aaacaacgga acagaagcaa
    33901 caaaaggtag tgaagtgacg aaaaaagcct atgaactcct tcagaatgaa acaagcttga
    33961 attttattgg aaacgtagaa gctcgtgagc tactcaatgg tgtggctgat gtagtggtca
    34021 cagatggatt tacaggcaac gctgttttga aatctattga aggaacagca atgaatatga
    34081 tgaatctatt aaaatctgct attctaaatg aagggatcaa aggaaaaatg ggcgctatgc
    34141 ttttaaaaga cggcttacgt tctttgaaag cagaaatgga ttattccaaa catggcggtg
    34201 ccgttttgtt tggcttgaaa gcaccagtca ttaaaactca tggagcaaca ggccccgatg
    34261 ctgttcgcta tacgatccgc caaatccaca caatgcttga gacagatgta gttggacaat
    34321 tagtcgaaca atttgaaaaa agcgaagaat agtatttgga tatctgaagc gatccgttcc
    34381 attgaaaaaa aggtggacat tcctgaaaaa tgccagtaaa attagccatg gtgtaggtaa
    34441 ttgacaagtg tatgattctt ggaggtgaaa aagttgactc gcgaagaagt atttaacaaa
    34501 gtagcgaaaa ttatttcaaa tcattttgag atagataccg ataaagtgac ggatgaattg
    34561 aacatcaaag atgatttaaa agctgattca attagtatta tggaattcgt tttagagctt
    34621 gaagatgaat tcggtacgga aatttcagat gaagacgctg aacaaattga aacagtcggt
    34681 ggcgcagtag attatatcag cagccatctt aaataatttc aaataaaagc aaagaaggat
    34741 aaaaagctgc atgcagcttt ttatccttct ttttttaatc tttttgacac gtaaaagtca
    34801 tttttctgat aattctggcg ataaacagaa attatgttgc aaagtaaagc ggttttctat
    34861 ataattatta ataattcatt acacaaagat aagaataacc tttgttataa aacgatttat
    34921 tctgtgtaat gtcctttttt attaagaaaa acttccattt aggaagcaga gaggggtatt
    34981 cgattgtccg ataaccaatt attagaagtt cagaacctac atacaggatt ccgactcaaa
    35041 gatgactatt acgatgcagt agatgacgtt tcatttactt tagacaaaaa cgagatttta
    35101 gcaatcgttg gggaatctgg gtgcgggaaa agtaccttgg ctactacgat tatgggatta
    35161 cttgatccta ataacacaaa aattacaggt gagattttat ataacgatct gaatttaatc
    35221 gacctgaacg aaacgctcta taataaaatc cgcggaaatg atatcggaat gattttccaa
    35281 gatccgttgt ctgcattaaa tcctctcatg agaatcgaag accagatcaa agaaggactt
    35341 tcctatcaca caaagatgaa tgcagaacag cgtcaagctc gtgcactaga attgctagaa
    35401 caagtaggga ttcctaatcc tgcgagagtt ggacgccaat atccccatga actatcaggc
    35461 ggtatgagac aacgtgtcat catcgcaatc gcaatcgcct gcaaacctcc aatcgtcatt
    35521 gcagatgaac cgacgacagc cctagatgta accatccaag cacagattct tgatttactg
    35581 aaagaccttc aggaagaaac aaaaacaggg atcatcttga tcactcatga cttaggagtc
    35641 gtagcagaaa tggctgatcg agtggcagtg atgtatggcg gacagttcgt tgaagtagca
    35701 ggtgtaaaag aactgtttga tcatcctaag catccttata cacgctcttt actgaactct
    35761 ataccacaag aaggaaatca tgatgcagaa ctccatgtga tcgaaggtgt cgttccttct
    35821 ctgaaaaaca tgcctcgtaa aggttgtcgg tttgcaccac gtattccatg gataccagca
    35881 agcgatcatg aagaagagcc tatccttcat gaagtcgcac cgaaccactt ggtacgttgc
    35941 tcctgctata agcatttcca ctttagagac gaaaaaggag aggtgtagat gacagaatta
    36001 atcacaatca aagacctgaa agttcattac cctattcgca gtggattctt caatcgagta
    36061 acggatcatg tatatgctgt agatggtgtg gattttatta tcgaaaaagg aaagacatat
    36121 ggtttagtgg gagagtctgg ctctggaaaa tctactacgg gaaaagccgt tgtgggatta
    36181 gaaaaggtaa cagccggaca aatcatgtat gaaggaaaag atgtaacgaa gcgaagcaac
    36241 cgcaaaaaaa tgggttataa caaggatgtg cagatgatct tccaagattc aatgtccagt
    36301 ttgaatccta aaaagcgagt gttggatatc attgctgaac ccatccgaaa ttttgaacgg
    36361 ttaagcgatc aagaagaaaa gaaaaaggta aaaggactgc tagatatcgt tgggatgccg
    36421 gaagatgcgc tatataaata tcctcatgaa ttttcaggcg gacaacgtca acgtctagga
    36481 gtagcccgtg ctgttgctac caatccaaaa ctgatcgtag ccgatgaacc tgtatctgct
    36541 ctggatctat ctgttcaagc gcaagtattg aatttcatga aacgaatcca gcaagaatac
    36601 ggcctcagct atttattcat ttcacatgat ttaggtgtag taaaacacat gtgcgacaat
    36661 atcgcaatta tgtacaaagg tcgatttgta gaaatcggta caagagaaga catttataat
    36721 gacccgcgac atatctatac gaaacgtctg ctttcagcta ttccgcaaat cgatgtagaa
    36781 aatagggaaa gccacaaaga aaatcgacga agagtagaac aagagtatat ccaaaaccaa
    36841 aaagaatact atgatgcgac tggtcgagtc tacgatttgc gtacgatcac aactacacac
    36901 aaagtggcgt tgaaagatgg aggtgcaagc taatgtggaa aacgattctg cgacgtgttt
    36961 tattgatgat cccgcaagtt attattttaa gtgttctgat cttcttatta gcccaagcga
    37021 tgccggggga tccatttacc ggtttgatca atccaaatca ggatccggca gtgatcgaac
    37081 agatgcgaca agcagctggc ttgaatgacc catggtatgt ccagtacttc cgttggatcg
    37141 gaaatgcttt gcaaggcgat tttggtcaaa gtttcttata caaacgctct gttgctgact
    37201 tgatcggcga acggatcatc aacactttat atctatcgat cttgacagtt gtccttactt
    37261 atttgattgc tgtgcctctg gggatgcttg ctggccggta tcaaaattca atcttggata
    37321 agattgtcgt aatctacaac ttctttagtt ttgctgttcc tttatttatc ttcggcttga
    37381 tcatgctatt tgtttttggt tatcgtttag gctggttccc aacaagtgga tcacaaacac
    37441 taggtttgca aggtggattt atcactcaat ggatagaccg tttgaaatat attctgttac
    37501 catcaattac ccaagcattt ttggcgactg ccgtcacaat ccaatatctg cgaagtgaag
    37561 taatcgattc gaaatcgctc gattttgtca gaacagcccg ttccaaaggg gtaccgacga
    37621 ataaagtatt cagtcgacat attttccgaa atgcagcgtt gccgatggct tctcaaatgg
    37681 ggtatgaaat cacttcgttg attgctggtt ctgtagttat cgaaaagatt tttggttatc
    37741 cgggagtcgg aaaactattt attgattcca tcggccaacg ggactatacc gtgatcacat
    37801 ccttagtatt gattttagga atcgcgacgc tagtggggac attactttca gatatcatca
    37861 tgagtatcgt tgacccgcgt attcggattc aataaaacca atcagaaagg agaatggatc
    37921 gatgagtgaa aaaaacaata cagcagttca agaaagtatt cctcctatgg ggatacgaat
    37981 gattgctcga gaatttaaaa aagataaaat tgctattttt tcattaggtt tgctagtggt
    38041 tttattatta gtgatcttta tcggtgcgat gctgaccgat caagacaaag tgatgtatgt
    38101 tagtatcttc gacaaatacg ctgcaccagg aacagtttcg acacaaggac aaaagttcat
    38161 tctgggagca gatgaaggcg gacgtgatgt attaggacaa ttgatcatag gtgctagaaa
    38221 ctctgttacg atcggatttg cgatcacgat ccttacttcg atcatcgggg ttggattagg
    38281 gatcatctct ggctactacg gagggatcat tgacaacatc ttgatgcgga tcgttgattt
    38341 tatcatgatt ctaccgatca tgctgatcat tatcgtcttc gtatcgatta tttccagata
    38401 caatgtctgg tcctttatat tcatcatgag tgcattttat tgggtagcta aagcccgatt
    38461 attccgaagc aagacattat ctgaagcaag aagagattat gtcagtgcct caaaaacaat
    38521 gggaaccaat gattttaaaa tcatgttccg cgaaatcatg ccgaatctaa gttcattaat
    38581 cattactaat ttgacgatga actttgcggc aaacatcggt atcgagacaa ctttgacttt
    38641 cttaggtttc ggcttgccgg caaatgtacc tagtttaggt acattgatcg gttatgcaag
    38701 taacggagaa gtcttagtga atcggcaatg gatctggtta cccgcatcaa tccttatttt
    38761 agttttgatg ttgagtatca attatgttgg acaagcattc aagcgttctg cagatgcgcg
    38821 ccaacggtta ggctagtata gaagcgaagg gggaaatagg aatgaagaaa acgatttttg
    38881 gatttgcagc gctgatgtct gctgtcgttc ttgcagcatg cgggggcgga ggtacagata
    38941 cctctggaaa caactctgga ggcaacggca ataacaaaga ggaattaact tttccaattg
    39001 caacaaaaaa taatgatgat gcaatcgaag acggcgaatt agaagctgcg gtcgcaacgg
    39061 acagtcagtt taaaggattg ttccagtggg agttttacca agattcatat gatgcagctt
    39121 ttatggctcc ttctcatgaa ccgctatttg caaacgatga cgatttcaga atcacagacg
    39181 acggagctgc aacgcttgat ctagatgaag atacgaaaaa agcgacaatc aaagtaaaag
    39241 acaacgtcaa atggtcagat ggtgaagatg taacggcgga cgatgtgatt ttcccttacg
    39301 aaatcattgg gaataaagat tacactggta tccgttatga cgacactttc cgtaacatcg
    39361 taggtatgga agaatatcat gatggaagtg ccgacacaat ctccggaatc aaaaaaatag
    39421 acgacaaaac agttgaaatc gaatataaag aagtaaaccc aagcatgctt caatcgggtg
    39481 gtggtatctg gtcatatgca gcaccaaaac atacgttaga aggtgtagca atcaaagata
    39541 tggaatcaag cgatcaagta cgtaaaaatc cagtgacttt tggcccttat tacatgagta
    39601 atatcgttgc aggagaatca gtagaatact tgccaaacga gcactactgg aatggtacgc
    39661 ctaaattgaa aaagatcacg atgaaatctg taccgatggc aagtgcaact gaagcattga
    39721 actctaaatt atatgatatg attttccaaa tgccaacaga tacttacgat acctataaag
    39781 aaattgctgg ctatacaaac ttaggccgtg aacaaacttc ttatacttat ctaggattca
    39841 agttaggtaa atggaatgca gatacaaaga gtgtggaata caatccagat tcaaaaatgg
    39901 ctgataaatc attacgtcaa gcaatgggct atgcattaga taataatgca gtaggtgaac
    39961 gtttctatgc aggtttgaga agcaatgcga cttcattgat tcctccagta ttccagtcat
    40021 accatgatga tgagatgaaa ggattcacac aagatctaga caaagcgaaa aaactattag
    40081 atgatgctgg ctacaaagat gtggatgacg atggtttaag agaagacaaa gatggaaata
    40141 aactaacaat caactttgct tcgatgtccg gtggggaaac agcgcaacca ttggcagatt
    40201 actatatcca acaatggaaa gaaattggtt tggatgtcca atacacaaca ggtcgtctga
    40261 tcgaattcaa ttctttctat gataaattag aaaacgatga tcctgaaatt gatgtttacc
    40321 aagctgcttg gaatacagga actgatccaa atccaagtgg actatgggga ccaaatgctg
    40381 cgttcaacta cactcgttat gaatcagaag aaaatacaaa attgatcaac gatatcagtt
    40441 caagcaaagc atttgatgaa gagtaccaaa aagaagcata tcaaaaatgg caacaatatg
    40501 catttgaaga agcatttgcg attccaacgt tgttccgtaa tgaagtattg ccagtcaacg
    40561 accgtgtcaa agactgggat tggtcttatg atgtaccaaa cccatgggca aatgtttctg
    40621 ttacagcaga ttcacgttca taatcactaa aaatgaccga aaaagggcga tgtccttttt
    40681 cggttttttg atatttttct gtttatttct ctaatattca ggtcaaggcg ctgatttcat
    40741 gacttctcca ataaacggtg ttcaaagtcc agtttctcgg tattaagtca aatttatgca
    40801 aaaatatgga aagccatttt cacaaaattt tttcttcata ctcgaaacta aacgaatttt
    40861 tcacaacttc tcgtcaatca ctaggattat taaagcttta cttacctttt gaccgaacca
    40921 tttcaaattt gacaaaaaac cagtataata aggaagtatc gaaaaaatat ccataaaagg
    40981 aatgtggaaa atggacaacc agctgacaaa agaactaaaa gaaaactata acatcgtttt
    41041 tcacgatcta aatttgcttg aacaagcttt tacccattca tcctatgtga atgagcatcg
    41101 caatctgcaa ttatcggata atgagcgact tgaattttta ggagatgccg ttttagaact
    41161 gatggtttct cagtatttat tccgtttata tcctcatttg ccagaaggaa aattgacaaa
    41221 aacacgagct gcgatcgttc gcgaagacag cttgtcgaaa tttgccaaag aatgtcactt
    41281 tgatcaatat gttttgttgg gcaagggaga agagaattca ggaggacgta cgcgaccagc
    41341 attgctttgc gacctattcg aagctttttt aggcgcgtta tatcttgacc agggattcga
    41401 cacgacccat gcattcatcg aaaaagtgat ttttcctaaa gtcaaagcag gtgctttttc
    41461 acatgagatg gatcataaaa caaaactaca agaagtcttg caaaaatcag gtgatgtatc
    41521 aatcgaatac cgattgatca atgaagaagg tccagctcat gaacgagtat tttggatcga
    41581 agtttatgta gatgatcaat tgattggcac tggacaaggg aaatcgaaaa aattagcaga
    41641 acaagctgcg gcggaaaatg cattggcagc actgtaaaag taagtgaggg aaagctttgt
    41701 atttgaaacg aattgaaata gccggtttta aatcatttgc agacaagaca gtcattgatt
    41761 ttgaaaatag cgtcacagca gtggttggac caaatggcag cggaaaaagc aatattacag
    41821 aggctatccg ctgggtatta ggagaacaat ctgctaaaag tctgcgaggc ggaaaaatgc
    41881 cggatatcat ctttgccggt tccgataccc gtaagcagct gaatattgca gaagtcacag
    41941 tgattttaga caatagtgat cattatctgc cattggagta caatgagatc agtgtaaccc
    42001 gccgttatcg acggactggg gaaagcgagt ttttcatcaa taaacaaagc tgcagattaa
    42061 aagatatcca agaattgttc ttagattctg gattaggaaa agaatcgttc tcaatcattt
    42121 ctcaaggaaa agtagaagcg attttcagta gtaaaccgga agaccgtcgt gggatctttg
    42181 aagaagcagc tggtgtcttg aaatacaagc agcggaaaaa gaaagcagaa cagaagcttt
    42241 ttgaaacaga agacaacctc agccgcgtcc aagacatcat ccacgaattg gaagaacagt
    42301 taactccttt ggccgcccag agtgaggcag caaaggagtt tttacggcta aaagaaacac
    42361 tgactcaaac cgatgtctct ttgatggttg ctgaaatcaa gacagcaaaa aaagattggg
    42421 acaacaaaca agcacagctt gcgaaattca atctagaatt aggcaaacta tctgagtcga
    42481 tccaagaaca agaatccatt ttggcgaagc agcgtaaaga aaatgcgcaa gccgatcgtc
    42541 tgatcgaaaa aaatcagcaa gtattattag atctttcgga aaaactgaag cagacagaag
    42601 gacaaaaaga tgttttgcag gagcggacaa agcatacaca aaaaagctcg caagaatatc
    42661 aaacttcatt agcagaagca caaaaaaagg ttaaacattt tgaaaagctt caagaaagct
    42721 tgatgaaagc agcagcagaa aaagaaacag agatccaaaa agctgaagca aatctcataa
    42781 aaacacagca agaattagaa aaataccaaa aatcaacgaa agaattattg gcagaattaa
    42841 gagatcaata tgttgatttg atgcaagaac aagcagctgt aggaaacgaa ctgaagtatc
    42901 tagaacgtca atatatacaa gaaacagcaa aaagcaagca gacgttagcc aagcaatcgg
    42961 aagtagaagc atcagtagac cgattgatct tacaaaaaga agaattgaca caaaagcagg
    43021 cgcagctaaa atcatcttta actgaaacaa aagagaagtt agaaatgatc caacaaaacg
    43081 ggaaaaagtt ccaagaagca ttagcaaaag aacagcctaa aatgtatcaa ttaatgaatc
    43141 aagtccagca gctacgtgct cgtaagaaaa gccttcaaga aatccaagaa aactattttg
    43201 gtttttacca aggggttcga ttggtcttgc agcataaaca gcagctttcg ggtattgtcg
    43261 gagcagtggc tgaactgatc gatgttcctg cagatttcac gttagctatc gaaacagctt
    43321 taggtggggc agcccagcat gtcatcgttg aaaatgaaaa agatgctcgg caagccatca
    43381 cgtatttgaa acagcaaaga ggaggaagag caacattcct tccactgacg acaatcaaac
    43441 caagacaatt accggctcat atattgacac aagcagcagc tgtagaaggg tttataggga
    43501 ttgccagtga acaagtcagt tatcccgatc aaatccaaac tgtggttcaa aacctgctag
    43561 gaacgatcct tttagccaaa gacttaacta gtgccaatgc aatcgcgcaa acgatccgct
    43621 atcaatatcg agtggtttcc ttagaaggtg atgtgatgaa tgccggagga tccatgacag
    43681 gtggagcgaa taaacgagga aaccaaggca gtttgtttgt acaaaatcaa gaattgaaac
    43741 agttgactag tgaatttgaa gaagcagata aacaactcca agcacaagaa aaaaaagttc
    43801 aggagctgca acaagaaaca gctcgtttag ccgaagaaca agaagttttg cgtacacgag
    43861 gcgaacaatt acgttttgaa gaacaagaag cgacaaatca gctacaaaac atcataaatg
    43921 aacttgaacg ttttgaaaaa gaaaagcaaa tctcaacttt tgagacacgt gaactgcagc
    43981 agtttatcga agattacgaa aagcaaacga acgaattgaa agacaaacaa acagatcttg
    44041 aatcgcaacg acaaaaaatc gacgaagaaa tcaaatcgtt aagtcaagaa agcgaccaga
    44101 tggaagccag aagagcacaa gtacaaagtc aaaaagcaca agaacaagca gatcttgctg
    44161 tcttgaaaga gcagtttaat catctgcaga tccagctccg cggtgcccga gtccaaaaag
    44221 cagaggcaac cgaacgtcaa gaagcgatag aaaagcagtt ggcgacattg acagcagact
    44281 tttctgatca tgaagtcaca gaagaaagtt tggaaaaaca aattaacgaa ttatctgctc
    44341 agcgagaaac attaaaagcc gagttggcaa aagccaaaga gcagcgagat agaaggcaaa
    44401 aggagatcga taagcttgaa gcagtcttgg cagagcgtaa tcgtgagcaa aaagcacgac
    44461 tgtcggaaca atccaaatta gaagtccaaa aagaccgagc tgaaatgatt ttagataatc
    44521 atttgaccta tctgcagtca gaatatcagc tgacatttga gaaagcttcc caagactatc
    44581 aagaaacaac cgatatcgaa gacagtagaa caaaggtttc atccttaaaa gaacagattg
    44641 aaaaactggg accagtaaac ttaaattcaa tcgaacaata cgaacaagtc agtgaaagac
    44701 atacattctt agcaacacag cgagatgatc tgttagcagc gaaaaaccaa ttgtttgaaa
    44761 cgatggatga gatggatgat gaagtgcgga caagattcaa agaagtattt gaagctatcc
    44821 gtcaagaatt caaaattgtc tttccaaata tgtttggcgg cggtaaagct gaattagtac
    44881 tgacggaacc aactgatttg ctgaaaacag gtattgagat tgaagtacaa cctcctggca
    44941 aaaaactgca aagtctaagt ttgctttctg gaggagaacg agcacttaca gccatcgcgt
    45001 tgctcttttc cattatccgt gtctgtcctg ttccgttttg tatcttagat gaggtagaag
    45061 cagcgttaga tgaagccaac gtgaaacgtt ttggtcgtta cttgagcgat ttccaagatg
    45121 acacacaatt tatcgtagtc acacaccgca aaggaacgat ggtcgcagca gatgtactgt
    45181 acggagtgac gatgcaggaa tccggtgtat caaaaatcgt ttctgtacgg atggaagata
    45241 tcaatgaaga ggcgaaaatc aacgaaacga aagtgtagga acgaaaatga tcaaattagt
    45301 agcaatcgat ttggacggta cattattaaa tgaacaaaag ctgatttcag atgagaataa
    45361 acaagcatta gcacaagcaa aacagcaagg ggtcaagatc gttctatgta caggacgccc
    45421 cttagctgcg atggcgcatt atctgcaaga attaggactg gtagatgaag gtgactacag
    45481 catcactttc aacggcggtt tggtacaaaa aaatgatact ggtgaaatca tggaaaagaa
    45541 agtgatggaa gtatcagata tccaacgctt atacaaactg gctcagcaat tggatctgcc
    45601 actagatgtt ttgtctgaca atgtggtttt acagctgcct agtgccccaa ggaagcaatc
    45661 cttgtacaat gtgttgaata agctgctgca attccaacca gcggatataa gcgatattac
    45721 agatgaattt gtgcttaata aggcagtaat cgcttatgac caagaagaat tagatcctaa
    45781 aatcaaagaa ataccagcag aattccatga aatgtatgag atcatcaaaa caagaagtat
    45841 gcttttggaa tttatgccaa aaggtgttac aaaagcttat ggtatttctt tattggcaaa
    45901 agatcttggt ttgaaacaag aagaaatcat ggcgatcggt gatgaggaaa atgatttacc
    45961 gatgatccaa tatgctggac taggcgtagc gatggagaat gctgtgcctt tcgtcaaaga
    46021 agcagcagat ttcgtgacag ccagcaatga agaaaatggc gtagccaaag tgatccaaaa
    46081 atttgtatta gagtaggggg agtcaaatgg gattttttga taaaatcaag cgtgctttta
    46141 ctggcgaaga gaaaacagaa gaagttgaag tacaggagaa gtacgacaag ggtttagaaa
    46201 agacgcgtaa aacatttggt gaacggatga atgagttgtt tgccaatttt cgaacagtcg
    46261 atgaagactt ttttgaagaa ttagaagaaa cacttatcgg tgcagatgta ggttttgaaa
    46321 cagcgatacg aattaccgaa gcattacgtc aagaagtgaa actcagaaat gcgaaaaaat
    46381 cggcagaagt ccaaaatgcg atcatcgaga aacttgtgga tctctatgaa gcggaaggaa
    46441 tcaatgaagt aaatgaacta aatatccaag aagacggact aacagttatt ttgtttgtcg
    46501 gtgttaatgg tgtagggaaa acaacaagta ttggtaaact tgcccatcag ttcaaaaacg
    46561 aaggtaaaaa agtattgatg gcagcagcag ataccttccg cgctggagca atcgaccagt
    46621 tagttgtctg gggcgaacgc gctggtgttg aagttgttcg tggcaatgca ggaggcgatc
    46681 cagcagctgt cgtattcgat gctttggaaa gagcaaagac agagcaggca gatgttttac
    46741 tagttgatac tgcaggccga ctgcaaaata aagtgaactt gatgaacgaa ttagaaaaaa
    46801 taaaacgagt aatccagcgg gaattaccag aagcacccca agaaacactt cttgtattag
    46861 atgcaaccac tggtcaaaac gcgatggttc aagcaaaaca attcaaagaa acaacagatg
    46921 tgaccggtct tgttttgaca aaattagatg gtacagcaaa gggcggtatc gtacttgcta
    46981 tccgtaatga attgcattta ccagtgaaat tggtcggtct tggtgaagga atcgatgact
    47041 tggagccgtt tgatccaaat gattttgtcg ttggattgtt caaaggatta ctaaaagaag
    47101 aataacccat gtctaaaaag caggagctgt gacaaaagtt gcagctcctt gtttgaatga
    47161 ttgtttttaa catagctgtg tttttgatgc cttcattttt atacttattt gtaagcggtt
    47221 ctcaaacaat ttcgttcttt ttcttagact ttttgattat aattagattc atatgaattg
    47281 gaggtcactt aatgatgaat tttaagcaat tattattgca tgtcaatgcg cgccctttta
    47341 tcgaccaagc tcgatttggt atagaacggg aaggacagcg ggttgatctt gcaggaaatc
    47401 tagcaaaaac cgatcatcca gcaatctttg gcgatcgatc ctatcacccc tatatccaaa
    47461 cagattttag cgaaacacaa acagagatga tcacccctgt taccgattct attcccgaat
    47521 tatttcagta tctcgctgct gtttatgatg tgactgctcg ttctataccg aaagaagaga
    47581 tgatctggcc attaagtatg ccacctgcct taccggaaaa agacgaagag atcattattg
    47641 caaaattaaa aaatttcgaa gatgtcttgt atcgacgtta tttagcaaaa gaatacggga
    47701 agcgaaaaca gatggtaagc ggtattcatt tcaattttga atttggcgat gagttgctaa
    47761 gaacattgtt cagccatcag gaagaatttc aagatttttc cgaattcaaa acagaacttt
    47821 atttgaaaac agcgagaaac tttatgcgct atcgctggat gatcacttat ttattcggtg
    47881 cttcaccaat gagtgagaag aactactttt tagatgaatc acatccgcaa gaacctgttc
    47941 gcagtattcg caacagtgcg ctgggctata cgaatcatcc aaatgtgaaa gtttcctatg
    48001 cttctatgaa acagtattta gcagatatcg agcgaatgat cgaagaaggg aaactttcgg
    48061 aggagaaaga attttataca ccgcttcgtt tccgaggcgg aaagaaagtc gcagatttag
    48121 caacaacagg tgttcgttat attgaattgc ggaatatcga tttgaatcct tatgcaagat
    48181 tgggtatcaa tccagagcaa gttcggttct tacaattatt cctgatgtac atgttatgga
    48241 cagaagaaaa agaagactgt gaccaatggg tagcagaagg tacgactcga aataacaaag
    48301 tagcactaga acaaccatca gatcaaacag aatttcatca agaaggtaga gaaattcttg
    48361 aaggaatgaa gcaaatgctg gttgaattgg attggttgga ttctctttat ttagtagagg
    48421 aagcattgac tcaaatggat catcctgaac aaacgttagc agcgaaactc tatcaagaag
    48481 cacagctatc gagtcagcaa gaagttgctg tggcattggg acatcaatat tataaagaaa
    48541 gccatgaacg tccctatcag ttggccggtt ttcgcgaaat ggaactttct actcagatat
    48601 ttatgttcga tgccatccaa aaaggtgtcc aagtcaaagt attagatgag tcggatcaat
    48661 ttttgcgtct gcaattccaa gaccatgtag aatatgtgaa aaatgcaaac atgacaagta
    48721 aggacagcta tatcgtccca cttatcatgg aaaacaaaac agtaacgaaa aaagtcttaa
    48781 aagaagctgg gttccgggta ccaggaggcg cagaattttc atccatggaa gaagcggtaa
    48841 aagcttatcc gagatttgcc gagcaggcat ttgtgatcaa acctaaatca accaattacg
    48901 ggttaggtat cacgattttc aaggaaggag ctagtctgga agattatcaa gcaggtttag
    48961 ccattgcttt tcgtgaagac agttcggttt tagtagaaga atttatgccg ggaacggaat
    49021 atcgtttctt tgtgatcgac ggagaagtcc aagccatcat gttgcgagtc cctgctaatg
    49081 ttattggtga cagtatccga acagtgaaag aacttgttga agaaaaaaat agtgaccctt
    49141 tgcgtggaac gaatcatcgt gcacctttag aattgatcca attaggtgaa ttagaacaat
    49201 tgatgttgaa agaacaaggg ttgacgatcg aatcagttcc ccaagccaat caaattgttt
    49261 atctaaggga gaactcaaat atcagtacag gtggtgattc gatcgatatg actgatgaat
    49321 tttctgaagc ttacaaaaaa atcgccgttt ctgcggtaga agcactagga gctaagatta
    49381 gtggtatcga tttgattata ccagataaag aaatcgatcc tacaactgat aaaaaagcat
    49441 atgggatcat cgaagcaaat ttcaatccag ccatgcatat gcatgtttat ccttttgcag
    49501 gaaaaggcag aagattgacg atgaatgtat tgaaattgtt atatcctgaa gtattttaaa
    49561 agaaaaagct tttgacagat tgtcctattt gatgtcaatc tgatcaaaag ctttttttgc
    49621 atgtaaaaat ttatgtgttt tttcgtttta aaataaacga tcccagaagc tttttttctt
    49681 ttttggtttt tctttttcag ctggcgaaga gttttcttct gggtattttt tttcgatatc
    49741 aatcacaggt tcatttactg cttcttttgc tgttaagagc aatccgaatt cctcaggatt
    49801 tgcagtgacg taatcgttga ctacagtgaa acggctattg tttttcgttg ctagctggat
    49861 atattggttt tggacagaaa ggggtagagc cccattcaag agtatggaag catctggatg
    49921 ttttttgagt tcttttaaaa agttttcttt gttttttggt tggatcattt cagcaacagt
    49981 catactaaga tagcagcgtt ccctgaatgt ccctaaatat ttatgctgtt cttcagggtt
    50041 cactaaaggt gtaccataaa gtcccttatc caaatggctt tttacatcat cttgtgccat
    50101 aaaaaatacc ctccttcttc tctttgtctt tcttacgatt atagcatatt cccaataata
    50161 cctaaaacat atgaaaaagc ggttgctttt gttcaacgat tcacttaaaa taagaatgaa
    50221 aatcattctc aattagaaag gtggattgga tgcgctacgg acgactagta gcattattag
    50281 ttattttaag tagtatttca ttgttcatcg gagtatcgaa tgtacaattg tcagaactgc
    50341 tccagttaga tgggcaaagc ttacaaatct tgctagtgac tcgtttgcca cgaacgattt
    50401 gtttgatcct tgtgggagcg accagtagta tttgtgggtt gatcatgcaa catttgaccc
    50461 aaaataaatt tgtttcacca acgactgcgg gcacgatgga tagtgcgcgt ttaggtattt
    50521 tagtggctat gattttccta cctggtgcat ccttgattgt ccgatccttg acagcatttt
    50581 gctttgcttt tgcaggaaca ttgctttttt tatcccttac gcgattgttc ctccagaaaa
    50641 atcaattgat gcttccgttg attggagtca tgttagggaa tatcattggt tcagttgcta
    50701 ctttttttgc ttatcaattc cagttggtac aaaatatgac ttcatggctt caagggaatt
    50761 ttgcgactgt gatacgagga aattatgagt tattgtatgc tgttgttcca ttgttgctct
    50821 tggtttggct atttgcttac cgatttacga tcattggtct gggcgaatcg tttgcagtaa
    50881 atctgggaat agattaccag aagatgcagt ttttaggcat tggcttagta tcattggcta
    50941 gtgcagtcat gttgatcatg gtaggaagca ttccttttat cggagtgatc attccgaatc
    51001 ttgtctctag aatatatgga gatcaagtgc acaaaacaat tggcgtaact gctttagcag
    51061 gtagtttgtt cttagtattt tgcgatcttg tcgctcgcgt gttgattttt ccttatgaaa
    51121 taccagtcag tgtgatcgtt ggaatcatcg gtggggctat cttcctttat cttctgataa
    51181 ggggacgacg atatgcttaa aaaaaagcca tctattttta ttggtatgct tgttcttgtt
    51241 ttatttgtta caggaatgta cttgacgatc catacatatg gaaactggca ttttgcatgt
    51301 gcattgagga gtaagaaaat ttttgcattt atccttgtct cgctagctgt cagcagtgcc
    51361 acaatcagct ttcagacaat tacgcataac cagtttttga ctccttcagt attgggactt
    51421 gagtcgttat atgtccttgt ccaaacggtc cttttcttct ttatcggagg agtgacgatg
    51481 cttcagcaga caggtacagt ttttttctta ttgaacgttg ggctgatggt cgggctgagt
    51541 ctattgttat ttttctttct tttgaaaaaa atgagagcga atcttttttt attactgatg
    51601 tcagggatga ttttaggtac gctttttggt agtgtcagta cctttttaca agtcatcatg
    51661 gatccgaatg aatatgattt gcttcaaggc agattatttg caagctttgg caatatccag
    51721 agtcagtatc ttttgcctgc ttctttgatc attatcggct gctggtacct gttatttcga
    51781 tatcgacatg tgctagacgt tttccatctc ggaaatgaac aagcagtaaa cctcgggata
    51841 gacacagatc gttttgaatt tttgatttta tgttgtgtca gtcttttgac agggacatca
    51901 acggctcttg tgggtccagt tactttttta gggtttattg tcgcaaatat cagttatcgt
    51961 gtcttttcta cttatcgtca tggtcagcta tttatcggaa gctttctggt agctatgctg
    52021 tttctgacag gaggacagct cgcagtagag cagattttcg gctggaatac aacagtgagt
    52081 gtcgtcgttg aatttgtcgg aggaatttat tttgtcacaa aattgattcg cgaaaggaag
    52141 agtatcctat gattgattta caaaaggtca ataaactgta ccatggaaaa tcagtggtta
    52201 aagatgtaca gttaacgatt cctgaaaaga aattgactgc ttttatcggt ccaaatggtg
    52261 ctggaaagtc aacggtgttg tcgatgatca gtcgtttgat accaaaagat acaggtgaaa
    52321 tctatttaga ccataacgaa gtaaaggctt ggaaatccaa tgaattagcc aaaaaattat
    52381 cgatcctacg acaatcgaat gaaataaatg tccaaatcac cgtgaaagaa ttagtagaat
    52441 ttggacgatt tccttatacg aaaggaaaat tgacgacaaa agacgaggaa atcgttcagg
    52501 aatcactgga acaccttggg ttagaagaaa tagcggatca atcgattgac actctttcag
    52561 gcggtcaact ccaacgtgcg tatattgcaa tggtgctagc gcaagacacg gattatattt
    52621 tactggatga acctttgaac aatctagata tgaattttgc ggttcaaatc atgcagattt
    52681 taaaaaattt agtacaagaa atcgggaaaa caattgttat tgttcttcat gatatcaact
    52741 ttgctgcaag ttatgcagac gaaattgttg caatgaaaga tggacagtta tttacgcaag
    52801 gaacgaccca agaaatgata aaaaaagatg tattggatga attatacgaa atggacatac
    52861 ggatttgtga gattgaaggc cgcagattct gtctttattt ctaaaactaa cattaggaga
    52921 gaagagatga aaaaattagt agtaggtatc ttgattctag catcatttgg tttagcagca
    52981 tgtggaaata gcaacaacac cagtcaggca gacacaaaaa aaagcagcac acaaactgaa
    53041 actacattga cgataacaga tagtaatgga gaccaaatcg aggtgccaaa taatccagaa
    53101 aaagtcgtcg tattcgacaa tggttcttta gatacgatgg atgctcttgg tgttggagat
    53161 aaggtagttg gagcagctac aagcagcctt cctgagtatc tttcatccta taaaaaagta
    53221 gaatcagcag gtggaataaa agaaccagac ttagaaaaaa ttaatcagct tcagccagat
    53281 ctaatcatca tttctggccg acaaagagat tttcaaaaag atctaagtca aattgctccg
    53341 accattttcc tttctttaga tgcgaaaaat ccatgggaat ctttccagca aaatgtcact
    53401 gctttagggg agatctttgg taagcaagaa gaagctaaaa ctcaattaga ggaattatct
    53461 agtgcaatcg atcagacgaa aaagaaagca gaagctactg acaaaaaagc actagtcaca
    53521 cttgtgaatg aagggcaatt atcagcctac ggatctggtt ctagattcgg tttcatccat
    53581 gatttatttg gctttgaaca agcagatgat caaatcgaag catccactca cggtcaaagt
    53641 gtttcttatg aatatgtgct ggaaaaaaat ccggatatct tatttgtagt agacagaaca
    53701 aaagctattg gtggagacga cagcaaagat gatatttctg ccaatgaact ggtagcacaa
    53761 acgaatgctg gtaaaaatca gcagatcatc tcccttgaac cagatgtctg gtatttaagc
    53821 gggggcggtt tagaatcaat gaaactgatg atcgaagatg tgaatcaagc atttaaatag
    53881 cataaataac agaggattaa actctcatac tatgtaacaa taatgtttct tacattctta
    53941 aatagcaaga gcctatcctc aaagaagtta tcaaaaacca tccgtaaaat cgtattgatg
    54001 attttacgga tggtttttat ttatttgaga aatctttttt caacgtgaat tcattatcta
    54061 catcatacgg gttgattcta gaatggggag tggcaaaaaa tagtgtgatc cattttttca
    54121 gcatcaagat cgcctccttt gatttgaatc aaaggtacaa agcgaaactt agataaaact
    54181 taaatttaga tatttttatt ttgtaccgat ctcttcttta aacgatgttc catctgctgc
    54241 ttctcggcaa taagtccact agactagaaa atatgagaag ctattttctg tccagcgttc
    54301 ttattactca aagcaaatcg cagttgtcac aatctcttct aaacaatgtt caatcagctg
    54361 accttcggta ttagctcaaa gcactgattt cacaacctct atttaggcat gtgtctcagc
    54421 aagcggttga tagttgatga tctgcatttt tcgataaaaa tcggcgggaa gcttactcca
    54481 ccgttttaat aattgataga aataactctc ttctgctttg attttatact cgcttaatat
    54541 caatccaaaa ctattcgttt tttttgaagc taatttttta taaatatcca atgctttggt
    54601 tttatcttca ttgacaaaga gtgtttttac ttcaatataa gaaccctcga tattttgtgt
    54661 ttcttcgata aaatagatgt tttttatata ttccttcata gtgattcctc caaagaagtc
    54721 ttttatttga taagtttact ctaccgaaga gttgttaata atccgtgaac aaaaagtaat
    54781 tctttttcca atagttgtta aaggtttttt attttttata gtattttttg ttatttaaga
    54841 aaggaataag gttaaacaaa taacaggttg ctttttgggt ctttttccgt tcaagaaact
    54901 gaacgaagaa aagggaaagt tgtctctttt gtgataagag aaatcataat aaacgaatga
    54961 cttactttcc aataaattga gaacagtcag aaccagacaa attagttgca tctgatgaat
    55021 aaagtgatta ttctatgaca tgaggtgata cagatgaaat tagaagtgac aaaagaagca
    55081 caagaagtat tgaagaataa attagaagca gacgatcaac ttttattaga tatcgaaaac
    55141 ggtgacggtc catttgcaga aagccaagtc agttgccaat tggatacggc atttcgattg
    55201 atcattgtca aaaaagatac acaaacagat ctaagtcttt attctgtagt ggctgataca
    55261 ccagttggac caatcaaaat caaagacagt gcaatacttt acatggatga tccatccaca
    55321 cttgctttag aaccaactta taatagttta caattaaaag gaccaagcgg cttgagaaaa
    55381 ggcaacttgc aagttgttcg aaaagactag agggataaaa tgggaaaatt ggcgatcatc
    55441 agcgacctac atgtggacat caataaattc ggcgaatcag agctgatgca gctttggcaa
    55501 gttttgcaaa agcacaagat cactcggctt catctagccg gagatacggc taataaagta
    55561 gaccgttgcc tagctgttgt cgaatttttc gtcaataaag gattatctac gacatttcat
    55621 ttcggcaatc acgagttggc agatgtgccc aatgaacaga tgatggacca ttttccagac
    55681 ccacattttt taaatgaacg gtatattgct ttaaatgagc agaccgttct tttagggatg
    55741 aatggctggt acgactatca gttttccgaa ttgaaagatt acgaggcaat cgtacgaatg
    55801 aagcgtttgt attggtatga tcggttcatc cagcgtggga aaacagatct cgaggtcaat
    55861 gcagaaatgc ttgcagctgc aaaaaatttg ctagaccagc tggaagcaaa aaaacttgag
    55921 gtgattttat ccactcattt cgttcctaaa cgggcattta tcatgtatca atctgctcct
    55981 tatgagcgtt ggaacaaatt aaatgcattt ttaggatcag aatcattcgg agaattactt
    56041 gaccgttatt caaatatcaa acaagtagtt tttggccata cgcatcgtcg ctttgaagat
    56101 aaatccatcg gtcacacgat ttacagctgc agaccttttg gttattatta tgaatggcag
    56161 ctgacccgag attttgtctt gaaagaacag ttgctagaca gctatgatcc tatgaaatta
    56221 cgtgtcctct tgcgtgccca tcacccagct tttcaggcct atcaaacaca acatttgcaa
    56281 gaggaatttg aacaagcgat gacaatcgtt ccgtattgag acagcattag taatgtgtaa
    56341 aagaaaatac cttgtaaaac ataaggataa gtaagagcag tagatccatt tatcgtggat
    56401 atgctgcttt ttttgcggaa aataatcagc gaatgtttat ttgatcggat gatttgtaac
    56461 tcgcagagta ctagatgcaa gcgtgtattc tttttttgaa cgataatcac aaaaaaaaat
    56521 ata
//
LOCUS       DACDXE010000012        52066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.012, whole genome shotgun sequence.
ACCESSION   DACDXE010000012 DACDXE010000000
VERSION     DACDXE010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.012"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            256..1101
                     /locus_tag="IWM23_000709"
     CDS             256..1101
                     /locus_tag="IWM23_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9883376.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1382..2602
                     /locus_tag="IWM23_000710"
     CDS             1382..2602
                     /locus_tag="IWM23_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAP9883377.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2599..5319
                     /locus_tag="IWM23_000711"
     CDS             2599..5319
                     /locus_tag="IWM23_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9883378.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5303..6247
                     /locus_tag="IWM23_000712"
     CDS             5303..6247
                     /locus_tag="IWM23_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883379.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6316..7326)
                     /locus_tag="IWM23_000713"
     CDS             complement(6316..7326)
                     /locus_tag="IWM23_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9883380.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7506..7850)
                     /locus_tag="IWM23_000714"
     CDS             complement(7506..7850)
                     /locus_tag="IWM23_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9883381.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7843..8277)
                     /locus_tag="IWM23_000715"
     CDS             complement(7843..8277)
                     /locus_tag="IWM23_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAP9883382.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8415..9152
                     /locus_tag="IWM23_000716"
     CDS             8415..9152
                     /locus_tag="IWM23_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883383.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9145..10356
                     /locus_tag="IWM23_000717"
     CDS             9145..10356
                     /locus_tag="IWM23_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883384.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10563..11342
                     /locus_tag="IWM23_000718"
     CDS             10563..11342
                     /locus_tag="IWM23_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAP9883385.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11412..12074
                     /gene="trmB"
                     /locus_tag="IWM23_000719"
     CDS             11412..12074
                     /gene="trmB"
                     /locus_tag="IWM23_000719"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAP9883386.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12172..13926)
                     /gene="ade"
                     /locus_tag="IWM23_000720"
     CDS             complement(12172..13926)
                     /gene="ade"
                     /locus_tag="IWM23_000720"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAP9883387.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14058..14987
                     /gene="cpaF"
                     /locus_tag="IWM23_000721"
     CDS             14058..14987
                     /gene="cpaF"
                     /locus_tag="IWM23_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAP9883388.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14944..15993
                     /locus_tag="IWM23_000722"
     CDS             14944..15993
                     /locus_tag="IWM23_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAP9883389.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15990..16268
                     /locus_tag="IWM23_000723"
     CDS             15990..16268
                     /locus_tag="IWM23_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAP9883390.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16243..16713
                     /locus_tag="IWM23_000724"
     CDS             16243..16713
                     /locus_tag="IWM23_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAP9883391.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16730..17032
                     /locus_tag="IWM23_000725"
     CDS             16730..17032
                     /locus_tag="IWM23_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883392.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17049..17420
                     /locus_tag="IWM23_000726"
     CDS             17049..17420
                     /locus_tag="IWM23_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAP9883393.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17392..17751
                     /locus_tag="IWM23_000727"
     CDS             17392..17751
                     /locus_tag="IWM23_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883394.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17923..18930
                     /locus_tag="IWM23_000728"
     CDS             17923..18930
                     /locus_tag="IWM23_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9883395.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18955..20139
                     /locus_tag="IWM23_000729"
     CDS             18955..20139
                     /locus_tag="IWM23_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAP9883396.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20576..22159)
                     /locus_tag="IWM23_000730"
     CDS             complement(20576..22159)
                     /locus_tag="IWM23_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9883397.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22737..22880)
                     /locus_tag="IWM23_000731"
     CDS             complement(22737..22880)
                     /locus_tag="IWM23_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAP9883398.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23102..23581)
                     /locus_tag="IWM23_000732"
     CDS             complement(23102..23581)
                     /locus_tag="IWM23_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9883399.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23654..23938)
                     /locus_tag="IWM23_000733"
     CDS             complement(23654..23938)
                     /locus_tag="IWM23_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883400.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24016..24210)
                     /gene="ssrS"
                     /locus_tag="IWM23_000734"
     ncRNA           complement(24016..24210)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IWM23_000734"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24282..25568)
                     /locus_tag="IWM23_000735"
     CDS             complement(24282..25568)
                     /locus_tag="IWM23_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAP9883401.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25706..26377
                     /locus_tag="IWM23_000736"
     CDS             25706..26377
                     /locus_tag="IWM23_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAP9883402.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26343..26831
                     /locus_tag="IWM23_000737"
     CDS             26343..26831
                     /locus_tag="IWM23_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAP9883403.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26844..27791
                     /gene="prmA"
                     /locus_tag="IWM23_000738"
     CDS             26844..27791
                     /gene="prmA"
                     /locus_tag="IWM23_000738"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAP9883404.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27792..28556
                     /locus_tag="IWM23_000739"
     CDS             27792..28556
                     /locus_tag="IWM23_000739"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAP9883405.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28789..31002
                     /locus_tag="IWM23_000740"
     CDS             28789..31002
                     /locus_tag="IWM23_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAP9883406.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31013..31459
                     /locus_tag="IWM23_000741"
     CDS             31013..31459
                     /locus_tag="IWM23_000741"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAP9883407.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31630..32889
                     /locus_tag="IWM23_000742"
     CDS             31630..32889
                     /locus_tag="IWM23_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAP9883408.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32978..33925)
                     /locus_tag="IWM23_000743"
     CDS             complement(32978..33925)
                     /locus_tag="IWM23_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9883409.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34319..35611
                     /locus_tag="IWM23_000744"
     CDS             34319..35611
                     /locus_tag="IWM23_000744"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAP9883410.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35626..37395
                     /gene="aspS"
                     /locus_tag="IWM23_000745"
     CDS             35626..37395
                     /gene="aspS"
                     /locus_tag="IWM23_000745"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAP9883411.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37504..38379
                     /locus_tag="IWM23_000746"
     CDS             37504..38379
                     /locus_tag="IWM23_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9883412.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38645..40528
                     /locus_tag="IWM23_000747"
     CDS             38645..40528
                     /locus_tag="IWM23_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9883413.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40603..40887)
                     /locus_tag="IWM23_000748"
     CDS             complement(40603..40887)
                     /locus_tag="IWM23_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9883414.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40877..41611)
                     /locus_tag="IWM23_000749"
     CDS             complement(40877..41611)
                     /locus_tag="IWM23_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAP9883415.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41740..42363
                     /locus_tag="IWM23_000750"
     CDS             41740..42363
                     /locus_tag="IWM23_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAP9883416.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42555..43676
                     /locus_tag="IWM23_000751"
     CDS             42555..43676
                     /locus_tag="IWM23_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAP9883417.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43683..46811
                     /locus_tag="IWM23_000752"
     CDS             43683..46811
                     /locus_tag="IWM23_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAP9883418.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46830..50024
                     /locus_tag="IWM23_000753"
     CDS             46830..50024
                     /locus_tag="IWM23_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9883419.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50170..50877)
                     /locus_tag="IWM23_000754"
     CDS             complement(50170..50877)
                     /locus_tag="IWM23_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9883420.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51642..52040
                     /gene="spxA"
                     /locus_tag="IWM23_000755"
     CDS             51642..52040
                     /gene="spxA"
                     /locus_tag="IWM23_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAP9883421.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT        17087 a         9056 c        10776 g        15147 t
ORIGIN      
        1 ttttatgaat atcgaagtta gtttttcacc taacttcgtt ttttatagtt ggttattata
       61 aaatagtaat atagctgatt tggattcaat tcatatttat ttataaatca aaaatacaga
      121 gaatagtata taaatgtcct ttcctattct taaaatgatg tttgtactta cttaaattag
      181 gtataattag tatatgtttt gtgagaaatt tagacttaca ataagcagtg aatttataat
      241 caacgtatag gagacatgga tgaaatgaaa ctaattggta tagatttaga cggtactctt
      301 ttgaattctg accagagcat tagtcaaaaa aatgcaagaa caatgaaaca tttaccggaa
      361 gactgttttc cattcatttg ttctggaaga gaagtagaag acattaataa tattctcaaa
      421 aaatctggtt taaggatacc agcagtaggg ttaaatggtt ctgttggatt tgatggagaa
      481 aaaaagatat ttgagtcttc cttcgataaa aatgatgtaa aaaaaattga ttctgtccta
      541 tcgaaatttc caaccaaaat atatacaaat ctcggaagtt atgaatctca gaattataaa
      601 aataaaatga aaaaaatttt tcacgaggta gggagtgaat tttctatcaa agagctaaac
      661 tatgagcttg agtatgaaaa atctatttgt tcaatccctt ataataatat tgaggaaatt
      721 atagaaaagg atgctatcag catttacaaa ttttttattt ttattcctaa tcgtcaattg
      781 aaagctaata tatttaacca tttgaaaaag atgctaaatg tgtctgttac tgaatcggca
      841 cctgtaaata ttgaaatcgt acctcaaaat gtatctaaag gtgtggtgtt tgatcgtttg
      901 gaatcaatct acaatttaaa gaaacctttg agaatcgcga tcggagatag tttaaatgac
      961 ttatctatgt ttgaaagtgc agatattagt tttgcaatgg gaaatagtca tcaagaaatc
     1021 atgaaaaaag ctgattatgt cactgtttca aatgataaag acggagtatc tcaagcattt
     1081 gaaagaattc ttaccatata aactaattaa ggtagtatag aacatagaat tctaaatacg
     1141 gaaaaatgga gcataaattc cagtacaaat agtacttctt cagtgtcaaa cgaagcgaat
     1201 ataacataga tatttaggat tgtgacaaaa atcatgtcac aatctttttt tcgaataaac
     1261 ttattttttg aagtcggcca tttatcgtaa tttctttgca taatacgaac aaattttcgg
     1321 ttattgatga aaaaaaacat tgaaaatggt atgataaaag aaaaaaagga ggaaattttt
     1381 aatggtgaaa tttatccatg cggcagattt acatatggat cggtcatttg aaggactgac
     1441 aactcttgat aaaactgtac aggaaaaact attgaagacg aatctgaccg ttttatcaaa
     1501 tatcattgaa caggcaataa taaataacgt tgattttgtt ttacttgcag gagataattt
     1561 tcatcaaaat cgtccttcat taaaaataca gaaacatttt tcagaacaga tgaaacggtt
     1621 agaaaagaac catatacctg tatttataat attcggaaat catgatttct atcaaaaaga
     1681 gcggtattgg ttctcctttc ctaaaaatgt ccatttattt acatccgaaa cggtagagac
     1741 aaaaaaaata acgataaaaa gtggagaaac tgtctctctc agtggattta gttatcgtca
     1801 accgtggatt caaaaagaca aagtgatgga atttccttct agagaactca cagattacca
     1861 tattgggttg taccacgggg aacctggcgt ctctcaaaaa ggaaactatg ctcctttcca
     1921 accctcaaag atgcaagaaa agggatacga ttattgggct ttagggcata tccatgtgcc
     1981 aacggtctta aatgaaaagc aaacaattgt ttatccaggt gcgcctcaag gtcatactaa
     2041 aaaggaacaa gcttctacat ctattctact agttgaatta agtaaaggaa gttgccaaat
     2101 acatccgatt aaagtagcag aggtctattg gaagacaaag gaaatatcat tgaggaaagc
     2161 aagaacgaca aaagaggtca ttgttcacat tcgtcagcag ctttcaaaag tagaggatgg
     2221 atttagttta attgagataa aattaaaaga ttatgaccat ctcaatacag atgttttgga
     2281 acggatacaa tcaggagagc tattagacta tttgttagag gaattctcag ataggataaa
     2341 tgatttcttt atttggcgta tttctctcat agagaatact ttttatacga agaccccact
     2401 tgctgcttct gctaaattga tggaacagtt atttcaatac tatcagacac ctcaagattt
     2461 ccaacaaatt ttaaatgaag tatatagtca tcaagaagcg gctagaatat tgagcgaact
     2521 gccggaatat caagaagaaa cacttgaaaa agccaagcag ctgcttaatc aagatttttt
     2581 atttgaggag gatcaggaat gaagatttta aggatcgaga ttgctgcttt tggaaaatgg
     2641 cgtcaaaaaa gttttgactt ttattcaggc aatcagctga tctatggggg aaacgaagca
     2701 ggaaaatcaa cgatttatca atttattcaa gcaatattat ttggtttccc ttcaaaaggc
     2761 cgtaaaaaaa aggattatac accaaaagac ggctcagcct atggcggcaa aatatggctt
     2821 aaacatcctg tatatggtga atttgctgtc gagcgctaca agcagcaaaa tcgcggaaaa
     2881 tcgaaggtgt ggctggggga tcaagtggga agtgatgagt tactagaaaa actgatcgca
     2941 cctttgacga aagagttatt ccagcaggta tttactttcc agcaagagca gttgatggaa
     3001 ctagatcatt tgagagaaaa agagctacat gatgcgctga tttctttagg catcacgggc
     3061 agttctcaag tattccaaaa gcggcaagaa tattatcaga aagcacaaca attattcaag
     3121 aaaaaaggcc aaaaattact aatcaatcaa aaattaaatg aatggaaaga gctgcaagaa
     3181 aaaattcatc aaaaacaaga acaggaagct tattttggca agttgatcca acaagaacag
     3241 gaatacgagt cgcaacagca gcagttattg gcacaattaa aagaggcgaa caaacaattt
     3301 tcattagcca gacagcaaga acttaatttt tctttatatg aagaatggca atctctcaaa
     3361 aaaaacagca ctacaggtct tcctgatgag caacttcttt tagatctgga gtctttctcc
     3421 aaacggtatc agcaaacaaa tgagcgtttg gaagaattag aagcaacact ggaaaagcat
     3481 agcgggatgg atcagcagtc agccagatat tatttttatt tagagcagga gcaggaacta
     3541 aaagatctcc agcaacttca atttacagcg gaacaattaa acgaagaatg gcaaagatta
     3601 caaattgaga ctagagaaac aaattcagaa ttgaagattt tggaacaaag gtggcattgg
     3661 cagaaaaatc cgttaccgga agaattttta tatgaagaag aatggaagca tcttctggct
     3721 gaagaaagaa ggattgctga tctagaaagc cagatttctg tgcgtctcca aatagctaaa
     3781 gaacagcaag agatgattga agcagaagtg aatgacttgg aagagaccta tccagcatta
     3841 ttacaaaatc agtcagagat agcaccagtc tcttcatcta aaccgtatat gctgatggct
     3901 ggcggagcaa taatcattgc aggtatattt gtaccaatgc ccttgaaagt gatcctttta
     3961 ctaattggtg caggcatgat tggctatttc ttctacaaaa ggcaaccaaa aattaaaact
     4021 aaaaatgatt tctctgagat aaaaggaatg tggcaagaaa aactaggaca acttgatcta
     4081 gccttagctc aattagctga agcagaagaa aaacaaaaga gaactggaga agaaaaacag
     4141 attcttcagc aagaagtata tcgttttgcg gatcatcggc acttaggaaa gatggatacg
     4201 ctcaaactca tgaaagaata tggacaggaa gtcatagatt atcagaaatt atatggcgta
     4261 tttgagcgga aacataaaag agaacaggaa atacaagaaa aactacgaca aatcgaccaa
     4321 aaatttgagt ttgttaaaga atggttaccg atacaagata agacaatttc agaaaaaatg
     4381 gtattgcttt cccgttttat acaagagatg gaacagatga aatttgccag aagctatcag
     4441 caaaatactt tattgaagca agaaatcaat caattgaaaa agaaacaaaa agtatggata
     4501 caagaataca gcgaacaatt gactcaagca ggcttatctt atccttcgga agtacctgtc
     4561 tttttacaaa aagccaaaca agccgaacag cagatgaaaa gatacaatga attatcggaa
     4621 atgcttactc cactatttcc tgaagagctg acaaaagaga aattatctgt aagactgcgc
     4681 caactgaaag aaaagcaatc ccaactccag caagaagaaa aagaagcaag tgaaaatcgg
     4741 caacgactcc aacttcaagt agaagagttg caaagagacg gcacattgga cgaactttat
     4801 caacaagcta gccggcaaaa agcaaaatta caggaattac tggaggaatg gggagcactt
     4861 gaaatcgccg ggacattctt aggtgatctt tcaacagaga tgagtgaaag acaattaccc
     4921 cagttgttaa gagcagcagg tgattattta ggtattttaa caaaaggaaa ttatcagaag
     4981 atattactga aaaatgatac actgacttta acagacggat atacagaatt tcccatctat
     5041 gagttatcta caggtacgaa agatcagctg attatggcga tgagatttgc ttacctctca
     5101 ttagaagcta accgttcgat atgcccgatc atcattgatg atggctggct tcattatgat
     5161 catcagcgaa agtatcaatt agctgaactt ttagctaaat ttggagaaaa gtatcaaata
     5221 atttgttttt cttctgataa agaaatggta agctattacc atgagatgaa ccaaatcgta
     5281 aaacaattag aaggagcaaa taatgaaaaa aattcgtgat ttagtaatcg atgagcagtt
     5341 tgaacaattt gtcctgatca aaagtgcaga tgtaagaatc gctaaaaacg gaaaaaaatt
     5401 tattgctttt actttccaag atacttcagg tactatcgat ggtaaatact gggatgcttc
     5461 ggaggaggaa atcaataaat ttacagccgg cacagtcgtt tttctaaacg gtaaaagaga
     5521 ggtttatcaa ggaaaccctc aagtaaagat tcttcatatg cgcttaacaa gggtagatga
     5581 gccaggagag cctagtttat acatggaaag agcccctttg aagaaagaag aaatggaaga
     5641 agaaatcaat caattgttgt ttgagatcac caatgcgcat tggaatcgta ttgttcgcta
     5701 tctacttaat aaatatcaaa aacaattttt tgagtttccg gcagcaaaaa gaaaccatca
     5761 tgcatttgcc agtggattag cttatcatac tttgacaatg cttcacttag ggaaatcaat
     5821 cgccgatgaa tatcctgagc tgaatcgagc attgttgtat gctggtatca ttttgcatga
     5881 tttaggaaag gtgattgaac tttccggtcc tatgtcgaca gaatatacgc ttgcaggaaa
     5941 tttgatcggt catctggtat tgattgacga agaaataact aaagcctgtc ttgcgttgaa
     6001 aatcagcgaa gctgacgaag atgttcttgt attacgtcat atggttttag cacaccatgg
     6061 cttattagaa tatggctcac ctgtacgtcc aagaataatg gaagcagaaa tattacatca
     6121 aatcgacaat atcgatgctt cgatgcagat gatgttgact tcgattcgtc aaacagaacc
     6181 aggaaaatac accgatcgga ttttcggtat ggacaaccga agtttctatg taccaaaaga
     6241 tttatagcaa aaatttattt ttttcattca aaacaaaaaa agcgagacat aagtcttcgc
     6301 tttttttgtt ttcttttatt ttgatgaatc agttgtttct tcagttgaag attcttttgt
     6361 tgaatcagtt gattttgtgt cagaactctt tgttgaagct gtagtttcac ttgagtcttt
     6421 cgtacttgat gaagttgttg tgaatgcact caacacattt tcaaaggcat catctttgat
     6481 ttttacattt gcatctttta attcttcacc gattacttta gttgtgaatg cattgtcact
     6541 tagtttagtt tcagtagcga tatcttttag ttgatctttg tatttatcca tgtcattgcc
     6601 tttattttgg tttttcacca ttttgacaac gtagtattct gtcgcataag aagtaggatt
     6661 tgttgtagta ataacatcgg agatctcacc gtctttcaat ttgaaagctg cttcttttac
     6721 ttctgcagga atagttgttg tcgttgagtc gaatttaact ttaccgccat cttcttttgt
     6781 ctctgtatca gttgatttgt ctttggctaa tttagagaaa tcatctccat catccgctga
     6841 ttttttcaca tcttttgctt catcttcact tgataatttg atgatttgtg cttctacttc
     6901 tggatggaaa gatttccaag cagttttcaa atcgtcatct gtaatatcta cgtgagcttt
     6961 caagccagct tcaaaagcta aattgttacg aatatattct ttataagtat ctttcgtata
     7021 tccagcagct tctaattgtg attcaaaagt atcacctaat gatttcgctt gtttgtcgta
     7081 ttctgcatcc acttgcttat cggttacttt atcgccatat ttgttattga atactttata
     7141 aatgatcatc cgttgtacta aggattggtt cgatgattca agttttgctt catcatagaa
     7201 atcagataca gtgatcgttc cgcctttcat cgttgcgata tctttattag tatcgcctga
     7261 acaagctgct agagttaaaa cagccaacgc actagtagct gccaaaataa ttgatttttt
     7321 tttcattaaa atttgcactc ctgtaattag ttattatttt tatatattaa ctataccata
     7381 tcccatttcc tttaaaaacg ttgtcgtggt agtttcaaac aaaattcaaa atttcatcac
     7441 attcctagga aatacgcaaa cttttctcac ttatatagtt aaatctgttg agaaaataat
     7501 cattattata tttgttcagg atactctggc agatcagctt ttagtttttc tacttgttca
     7561 ttgatttgtg tgatacgtgg ttcggcttgg aatttaaaat cttcgatatc ttttttgatt
     7621 gcttcttgga aagcaggaat cacagattca gctgtttctt ttgtttcgat caatgcgctt
     7681 ttaaaatgat tcaggctgtt atttaaatca tttgtcagag ttgttgcttc ttccaattct
     7741 tcgatcagtt tctttcttgt ttcattcccg ctcctgggcg ccaataacag acctccgaca
     7801 gtgccagcag cagctccaaa taacagtcct ttgaaaaagt ttttaatcat cgcaggacac
     7861 ctgctttacg attgtttctt gaatagcttt tagttcttcc gatccataac tatcttgatg
     7921 attgacaaag tgaatagaaa aatcatcctc tttgctgtag cgagggacta aatggatatg
     7981 tgaatgaaaa acagattgat aggctagttc acggttgtta ttcaagatat taagaccttt
     8041 catttctgga aaagcttttt ccaatgcttg agcaatctta ggcacacgcg aaaaaacctc
     8101 tcctgcaagt tcgggatcat attcaaaaat atctgctaca tgacgtttag gcacaactaa
     8161 tgtgtgtcct ttcgttgctt gagagatgtc taaaaaagca tagaccttgt cgtcttcata
     8221 tattttatag cttgggattt cttgattgat gattttgcaa aaaatacagt tttccatata
     8281 aaacatcctt tctataaaga ataaatactt cagcttaact ttaccataag ttaaagctat
     8341 tgtaaggaaa aaaacaaaat accgtcattt tcttcgtggt ataataaata aaaataaatg
     8401 gcggaggaaa aagaatgagt ttaattatcg atcatgtcac aggggggtac ggacatctcc
     8461 ctgtgttaaa agatatcagt ttcgatgtac agcctggaga gatagtaggg ttgattggac
     8521 tgaacggagc agggaaaagt acgaccatca agcacattat cggtctgctg gaagcttcaa
     8581 aaggaacgat agaaatcaat catctatcct taaagaaaaa tacggaagaa tatcgcaaac
     8641 aaattgggta tattccagag actccttctt tatatgaaga attgacattg agagaacata
     8701 tcgaagtcac tgcgatggcc tacgatatac ctatggatat agcaatgaaa agagcagaaa
     8761 agttgttgga gacttttcgg ttatctaatc gtcttgactg gttcccagct aatttttcaa
     8821 agggaatgaa acagaaagtg atgatcttat gcgccttttt gatcgagcct tctttatata
     8881 tcatcgacga accattttta ggcttggatc cactagctat caatgcattg cttgaattga
     8941 tggtagaaat gaaaaaagaa ggagccggta ttttgatgtc tacgcatatt ttagctactg
     9001 cagaaaaata ttgtgatcgt tttgtggtct tacataacgg agagatacga gcagacggtt
     9061 ctcttgagat gttgagaaaa gaatttcagc tgccggattc ttctttggac gaaatctata
     9121 ttgctttaac aaaggagcag gcacatgagt agtttttttc aaaaacgttt agcaagacat
     9181 caaaagaaaa tgatgaaata tatgcgttat gtattgaacg atcattttgt tttagtttgt
     9241 cttttcttga ttggcggtgt gggattatat tattctaatt ggttgaaaac cctgcagcca
     9301 ccgtttgcaa ttggagggct cataatctct gtattttgga tgctctgttt attcgtgggt
     9361 aagatggcta cttttgcaga ggcagcagat attgtttttt tacttcctaa agaaaaagaa
     9421 atgcaaacct atttggaacg agcttttcgc tattcttgca tttttccgtt gatctttttg
     9481 atattggcat gcggattggc catgccatta gtggtggtta gtacggagca atccttttcg
     9541 atgtttttcg tctatctgat catattatgg agtttgaaat tcagtcatct gcaaatcaag
     9601 agagcagaat tgtttcgact agatcaacaa gttattcgtt attggaaatt aggctggttt
     9661 ttttcttctc tgatcttttt gtttctatca ttttattggc taatatgggc aggaatagca
     9721 ggtgcattag tacaggcaag tctatattac tggtttttgt ggaagcaaca agaaaaagga
     9781 ttggattggg agaaaatggt gcatgtagaa gaacataggt tgcatcagat ctatcaattt
     9841 atcaatttat tcacagatgt tcctgaaatt acggcaaaga tcaaaagaag acgctatttg
     9901 gatcctatat tgactcatat taaaagagaa tcaaaaaaca cttatcttta cttgtttgca
     9961 agaagattgg caagaggttc tgattttagt ggtctttatt tccgattagt aattataggt
    10021 gggttattga ttgccggagt agtcgacttt tattttagtc tgattatcgg tttcttattt
    10081 ttatatttga tcggtttcca actgttacct ttagcgaacc agttccgcta tatggttctc
    10141 gttcagcttt atccaatcca taagaatcag aagaagcagg cgattcagta tctactcaca
    10201 tgggtattaa ttatcgcgtc ggttattttt ggtttgattg ctgcagtcgt attagatggg
    10261 cagcagcgat gggttccgct agcggcttat ttgattgtta ctatgctctt tgttaaacta
    10321 tatgtaccaa ctcgtttgaa gaagatgtca gactgaaaac aaagctgtgt agggtaaaaa
    10381 tgagaatgaa aaatagagcg ttgacagtaa gaatgctgtt cgttctattt tttttgagaa
    10441 aagctggttt tttgaatggc aattaaatga aaaagaacgt atatgtgctt gacgtgtagt
    10501 gttcaggcga ctagaataat aagtactgca aaacaaagag taagaggacg ggagaatgca
    10561 tgatggagtt tcagttagac caagagtggc ggttacgtcc catcaaaggc gacacaggaa
    10621 aaacatacat aggcatgcgt gatgaggaca aagtctttat taaacgaaac acgactccga
    10681 tgttggctgc tctttcaagg gaaggaatta caccaaaact tgtttggaca aaacgaacgg
    10741 ggaatggcga tactttgaca gcacaagagt ggctggaagg tcgcttgctg actcctaatg
    10801 agattggtca aagaaacgac gtgattgatg ttttgtacca tttacatcat tcaaattctc
    10861 ttaaaaccat gctgacccgt atcggtggtc gtgtcatgac gccggagaag atgctgcaaa
    10921 aatatgaaaa agaattaccg gaagaactcc agaaaaatca atttctgcaa attgtccatc
    10981 gttttcttcg tgagcagatc ccgtcaatag ataaggaaaa cttatcagta gtacacggag
    11041 atgtaaacca tcgtaattgg ttagtatgta aaaattattt atatctagtc gattgggatt
    11101 caatcatgtt tgcagaccct gcggtggata ttgcaacgat tttaggaaat tatgtgccac
    11161 tgtctagctg gaatcagtgg ctgatcagtt atggcatacg cccgactgac gaaatgcttg
    11221 aaaaatttca ttggtatgcg gccatgaatt tgctccaaga aatcaccaga tattgcaaac
    11281 gtgaagatca tcgacggatg aacgaagaaa ttttacaatt aaaacgaatt ttcagtggct
    11341 gaggtttggc ccaaactctg cggagatttg gccagacctt tttaattaga ggaaaggaat
    11401 ggagataaat aatgcgtgtc cgaaatcgtc cgggagcagc ggaaatgtta gcggctcacc
    11461 cgaattttgt aattagtgat ccaacattgt ggaagggaaa atggaatgaa ttgtttgaaa
    11521 acgatcatcc aatccacatt gagattggta tggggaaagg ccagttcatc actggaatgg
    11581 caaaagccca tcctgaaatc aattatattg gagtagaaat gcaggtcagt gttgtttcga
    11641 ttgcactaga caagttgatc gaacaaccgc ttcctaacct gaaacttcta catgtggatg
    11701 gatcagcgtt gacagagtat ttcgctgaca gcgaagtaga tcaaatctat ctgaattttt
    11761 ccgatccatg gccaaaaaaa cgtcatgaaa aacggcgatt gacatataag acttttcttg
    11821 ctgtagatga acagattctt cgtccaaatg gagaaatcca tttcaaaaca gataatcaag
    11881 gattgtttga gtattctctg gcaagttttt ctcaatatgg catgatcctc aaacaggtat
    11941 ggttagattt acatcaaagt caatttgaag gcaatattat gacggagtat gaagaaaaat
    12001 tttcttcaaa aggtcaacgt atttatcgag tagaagctca atttcaagat aagaccaaaa
    12061 agaaaagtct gtgaaactaa ctttctgctt tcataaaaac attaatatcc ataaataaat
    12121 ctcagaaaat gaacattaca gatgtaatgt attttctggg atttatttga tttaaaatgt
    12181 atttatttca tcggtttttc cacaaggtgg aatcaattgg aagcgagaaa atgaaaataa
    12241 tcctttgtcg gtgatcttca attcgggtat gacagataat gttaagaaag aaagtgtcaa
    12301 aaaaggatca aaggttttag ctgctcctag ttgatgtgct ttagttatta attcattcaa
    12361 ctgttcatag acgatcgaaa aaggttcctg tgtcatgatt ccgcctatag gaagagaaac
    12421 acaagctatt tcctgctgtt tgttgacaac ggtcatcccg ccaccttttt gaatcaaaag
    12481 attagcagca tacagcatat cctcgtcgtt tgtcccaacg acaactagat tatgcgagtc
    12541 atgggcaatc gttgtcgcta tcgctccttg tttcagctga aatcctttca ctatgcctaa
    12601 tccaatgttt cccgtttgat ggtgacgctc gatcaccgca atctttaatt ggtctttttc
    12661 gattgaagga atgaatcgtt cgccttgtcg gtccactttt tctaccaaat ctttagtcac
    12721 tagactattc gggataattt cgatcacatg ggcttgtgaa gaagaaagaa gcagctctaa
    12781 cgaatgtgga tttaatggtt gagcgtttag ttttggcaga caacacgtat atgaatttga
    12841 atgatcagca aataaatggt cttggagttg cccagattga acaactaatg ttcccttata
    12901 gaaaacttca tgaattggca gcttggtaag atcgtctgtt aagaagaaat cagcttgata
    12961 cccaggggca atcgcaccta gtgttttcaa atcaaaacat tctgctgcat tcaatgttgc
    13021 catctgaatg gcttgtaatg gatcgattcc aagtgcaata gcttgacgga cgatatgatc
    13081 gattgatcct tcttttacta aatcattgat cagtttatca tcggtgacaa agaagcatct
    13141 tcttgaattt tcaggtgtga tcgccggtag tagcgatgcc agatcttttg caacggtccc
    13201 ttctcgaacc attaaataca taccaagatc caaacgttct ttagcttctt ttgcagttgt
    13261 agcttcatga tccgtccgaa tacctgctgc aggataaaca tttaactctt ccatgccgat
    13321 accagctgca tgcccgtcaa tttttttctt atgctggtgc atcaattgga tcttttctag
    13381 gatgtctgtt tcattatttg ctactgcttg atagttcatg acttcagcta agcctaaaac
    13441 ttcgggtttt tgtaaaaaag gggctaattt ttttgcattt aaatttgcac cattggtctc
    13501 aaaaggggtt acaggtacac aagaaggtag cataaaaaag atattcatga gtgtttgtct
    13561 tgcatcttct atcataaact caagacctgt actcccagca atgtttccta tttcatgggg
    13621 atctgtcact acagtagtaa ctccatgaag caaggatact tttgaaaatt cgcttggtga
    13681 taaaagtgag ctttcgatat gcacatggcc atcgatgaat cctggcacta gatattgccc
    13741 attagcatta tgtacgcttt ttccttcgta ttcaccgatt cctacgatca ttccttcgca
    13801 gattgctaca tctttttcca tgatttcttt cgtaaagacg ttgacgatct tagcatgctt
    13861 gatcactagg tcagcaggct tttttttact tgctacttcg atatatttcc ttaatgttgt
    13921 ttccaatgga gtgcctccca agataaattt ttataagtat aacataaatt cggcttttgc
    13981 caatattttt ttgtaaattc attttatttg aaacaaaaag atcaaaaaga cgtatgtttt
    14041 ttgtggaggg gagaataatg gaaataaaac gattatcgga tcaaatgata agtgaaagtt
    14101 ttgccaaaca tgcccaagac gtatatatat tgccagcttc agaagtattt tacatctatt
    14161 ttcgctatgg aaaaaaacga acattctgga agaaagtctc ttcggcagat ggacagcaac
    14221 tgatctcaag atttaagtat ttgggacaga tggatatcgg agaaaaaaga aaagctcagt
    14281 taggtgcatt tacttattct ttagaaaaac aggaagttcg tctgcggttg tcttctgtag
    14341 gagattacct tcaacgagag agtttagtga ttcggctatt atacggtact tttgaaacac
    14401 cccatcgttg ctttagttct cgagattttg caactatttt acagaaaacc gtcagacgtg
    14461 gattatacct tttcagcggg acagtcggat caggcaagac ttctctaatg tatcggttgg
    14521 ctttggaaga aaaattgcaa gtgattacta tcgaagatcc tgtagagata gaagaaccgt
    14581 tttttctgca gctacagatc aatccgaaaa tagcacagac ttatgataca cttttgaaac
    14641 tcgcattacg gcatcgtcca gacttgctaa tcattggcga gataagagat gaatcaactg
    14701 cacatgctgc ggtaagagca gcactaacag ggcaccgggt ttttgcaacc gtacatgcac
    14761 gcaatcttga aggtacattg gtcagaatcc atgaactgac aaagaaaaaa gaagagctat
    14821 ctgaatgttt agctggtgtc atttatcaag aacttttgga agatgccaat caccatcctt
    14881 ctgttttatg gagttatcaa tttatggagg aagaatcagt aaaaaaatca tgggaggaaa
    14941 gttatgaaga agctcaggaa aatatttggc agtcagaact attctaaagg attaacaaga
    15001 agccagcaaa accagtttgc acaaaccatg ggagaactgc ttttatctgg ttttagtctg
    15061 caagaatccc tttatttatt actcacgatc cgttcgtttc cacctgttat tttggcaaat
    15121 atccaacatc agctaaagga agggcaagcg tttagtgagg tactttcgca aataggatat
    15181 ggagcagagc aactggtaca gatcgagtta gctgaacgtc atgggaatct gccgacaact
    15241 ttgttggaaa ttgccaaaca gtttcggctt gttgaaggat ttcgcaatga tttgaaaaag
    15301 ctgcttgcat atccgatgct attgctcacc tttttgctag gtatcttgtt tagtatgcgg
    15361 ttgttcgttt tgcctcagct tattggttca ggaatggtca tgcaagagca ttggggtatt
    15421 cagctgatac aagtctttca ttggtatgtt ctactattca tcctagcggt tactctattg
    15481 gctttactag taaggtatcg tctgatgaag ttgtctcctg ttgatcagag tgaatggata
    15541 agcagacaat tattgattgg accgatatat tccttgtatc aatcggctta tttgtcacta
    15601 gagattggaa agctttttca tgaaggtttc gaattaaggc aaatcatagt ctgcttaaag
    15661 gaaacgaaac aagaaagtct gattcagcat cttgcctcta agttgattca aggattggag
    15721 gcaggtatgt cattggctga gcagttccag aattatcgtt ttctatcaga agaattcagt
    15781 aaaattgttt tacagggaga ggcaaaggga agtttaggta aagaactgat tttttacagc
    15841 gaatggacaa gaaaacagct tttcaaacgt cttcatcact ggatgcattt tattcagcca
    15901 atcatttttt tgggcgtagc ggctttgatt ttattagttt atgccgctgt tttattacct
    15961 gtttacggaa gcatagagga ggttttgcca tgaaaaagag aaaaatatct tattatagcg
    16021 gttttacatt gatcgaaatg ctaatcgtct tactgatcat cagtgtatta gtgttgttgt
    16081 tcgtccctaa tttgtcgcgt tataggaatc atgtagacca agaaagtcgt gaagcgatca
    16141 tccagttggt agacacacaa aaagagctct atgcgcttca aaataatgga agagttccta
    16201 ctgtggagga attgttgaat gaaggctata tcaaacgaga acatgcagaa atctatcagc
    16261 ggccttgaag gtaaggtttt agcatcagga tatacgttgt ttgaatcact cttactgatt
    16321 ttattactta caacgatcct ttcttttcca gttctcgcat tttctgcatg gaagaatgaa
    16381 ttggcagtct atcaattttt cggccaattt gaaaaacgaa tctattctac gcaaaaaatc
    16441 gctattgtga atcagatcca aacaggcttt tattggaata gtgaagagaa tcaaatcatt
    16501 tttcatgttc cgaatccaga taaaataaat tgggaagtat tagatattcc tgatgagatt
    16561 accttgaaac gccatgcttc tattactttt gctgctagaa ccgggaatga aagttcacta
    16621 aaagcttatc aattttattg ggaagaaaaa gagcggacga ttacgtatca attccagata
    16681 gggagtggtc gctatacaaa aagaattgaa taaaacagga tttgttttgt tggagtccct
    16741 tacagcgctt atgatcgctt ctgtctgtgt ctacttagtg agctttggcc agctgttttt
    16801 gcttcaggca gaaaagaaaa gtggggagga actgctattt attcgaatga tttatgaaga
    16861 tatcaagacg cagcggatgt atcacaaaag accagagcga agggaagaaa tgatcccata
    16921 cgatatccaa attgtcgata ataatggagt aatcgaaaaa gtgtttgtca atatggagaa
    16981 agaagcgatc agtattgaaa aaacaagtaa ttccagcatt tacgctgatt gaatgtctaa
    17041 ttggactgat gatattgagc agtctattgc tcacattcaa tcttttgatt cagcaaacca
    17101 tccaagtaag tgaatttttg cagcgtaaag atcaaaaaga gtggctaata tttttagcac
    17161 aattagaaga agaactcaag agcagtcaca atgtctctgt gaaaaatcag caactgcact
    17221 acacgatcga ggataaacag tatatcatcg aaagatatca ggcaatgata cgtaaaagaa
    17281 gaacttccgg cggacatcaa cctatgttaa caagtatcac tgagcttcaa ttattggaaa
    17341 aagaacacac aatttctttt tatgtacact ttgaaaatgg agaagatgga tatgccatat
    17401 ggacaaaaaa cgatcagtag aaaatgttat aagggctcta ttttattttc tgtcttgctt
    17461 ttatttcttt tatgtacgtt tttattgatg gcagcattag aaagctaccg tttgtcaatg
    17521 gatcttaata tacgaatcaa aaattattat atagcaaaaa taatgaagca gatggcttta
    17581 tcggaacagt ctacgcttgc cgaaagttcg gaaggagttt tttactacac aactggatct
    17641 gtaacatatc agtgggtaca acagtcccta ttcttagagg tggaagtctc accgtttatc
    17701 tttcggttca cagaagaagt aaaaaatgat acagatacta gtacagaatg atggaatgtt
    17761 cgtgaaaacg ccctcttttc tgttataaca ctgtttttaa taaaaaaatg tttgccgttt
    17821 cacaaataaa aaacagtgaa aacgattgaa attttgtcaa ttgttcgata gaattaaaca
    17881 ggtgaaaaaa ttgttataat atatctagaa atgaggtgca ccatgttacc agaaaaaatg
    17941 gaacaagggt ttaatcaaaa cctggaagct attcagcttt tacagaatgc attgggaact
    18001 tcctttttag aagcctatgt cgaaaatgca gaaaatttaa tagatgatta tcaagtacgt
    18061 gtggtggatg gagtgccgac aaaagagaca acgcaacgga tcacagctct ttatgaagag
    18121 ttgaagaaac tttctttcga gccagaagaa tggcgcagac tgtctcaatt acttttattg
    18181 aagggcagcc agacagaaca tttgcaggcg aaccatcaac tcactcctga tagtatcggt
    18241 tttttatttg tttttcttat tgaacaacta tacacaaata aaaaagcacc ggttaagatt
    18301 ctggatattg cggcaggaat gggcaacttg ttattaacag ttcttttaaa cctatcgaat
    18361 gcggggtacc aaacagaagg aattggtgtg gatattgatg atacattgtt ggctgtcgca
    18421 gcttctacaa gtgatttgac acaagcaaat gttcaatact ttcatcaaga cgggttgcag
    18481 gaattattaa tagatcctgt cgattttgca atcagcgatt tgccaatcgg ttattatccg
    18541 aatgacgaaa aagcaaaaga gtttttaaca agtaccgaag aaggccatag ttatgcacat
    18601 catttattgc tagagcaatc aatgaaatat gtgaagccgg atggttttgg tttgtttttg
    18661 atgccgagtg gatttttaga aacggatcaa agtgaagaaa tcaaaaaatg gtttaaagaa
    18721 gaaggttatc ttcaaggaat gattcaattg ccagatgaat tgttccgtaa taaacaatcg
    18781 caaaaaagta ttttgatctt acagaaaaaa ggaccacaag cacaacaagt aaaagaagtc
    18841 ttgcttgtga aacttgcttc attaaaagaa ccagagaagg tgacggaatt tttcaacgag
    18901 tttaaaaact ggaaatcatc tgctctgtaa tcattcaacg agaaggagag tcatatgtct
    18961 aaaacaattg caatcaatgc tgggagttca agtttgaaat ggcaattata tcaaatgcca
    19021 gaagaaaccg tagttgcgaa agggatcgtc gaacgtatcg gtttacaaga ttctattttt
    19081 accatcaagt acggcgataa tcaaaaattc gaacaaatca tggatatcga aaaccacgaa
    19141 gtagcagtaa aaatgctgtt agatcagttg attgaactgc aaattttaag ctcttatgat
    19201 gaaatcaccg gagtagggca ccgtgttgtt cacggaggag aaacttatgg ggattcagta
    19261 gtgatcgatg agaaagtaat ggaccgcatt gctgaattag cagaatttgc tcctttgcat
    19321 aatcctgcaa acttaatggg gatcaaagcg tttaaaaaga ttttacctga cattttgagt
    19381 gtggctgtat tcgatacatc tttccactca acaatgccaa agcaaaatta tttatatagc
    19441 ttaccggtgt cttattatga agatttcaaa gtaagaaaat atggcttcca tggcacaagt
    19501 catcgttatg tgtcggaacg tgcggcaaaa ttattgaaca aaccgatcga agaactaaaa
    19561 atcatcactt gccacttagg aaatggtgta tcgatcacag cagtcgatgg aggtaaatcc
    19621 atggatactt ctatgggatt cacaccatta gctggggtga caatgggtac acgttcaggt
    19681 gatatcgatc cagctgttct cccatattta atggaaaaat tagagattga tattgatgaa
    19741 atgatcaatg ttttgaacaa gaaatctggt ctgctaggcc taacaggatt gtctagtgat
    19801 atgcgtgatc ttgaaaagaa ctatgaaaaa gaacatattc gtttagctta tgacattttc
    19861 gtggaccgca tccgaaaata catcggcggt tatgtgacaa cgatgaatgg tgttgatgca
    19921 attgtcttta ctgctggtat cggcgagaat gacggacatg tacgtagtga aatcatcaaa
    19981 ggtatgtctt ggtttggttg tgaaatcgat ccagcgttga atgaattgcg tggtgaagaa
    20041 gtagatatct ctacaaaaga atcaaaagta agagtattag tgatcccaac agatgaggaa
    20101 ctaatgattg cccgtgatgt ggagcgtctg cgcggctaag ccaaagcatt cgttaggata
    20161 aaagatgaaa cgcatgcaag agggaattct tgcatgtgtt ttttttgcgg agaagaccgc
    20221 aaaatggtaa tttaacttca atttcccttc ttttttacag gatcttctta gggaattttg
    20281 cttggaaaag tggtcaaaag ggaaaagact ggcattttat ttattgattt tcaggaaaat
    20341 cccaatattt tattttcatt catctttata agggacatta catatgtaaa aagaaaaaac
    20401 atttttagat cagcctcgtg ggctgtcgac tttattcttc gctttatgtt tagctagaaa
    20461 accgatccga aacattcatt gatcgaataa taaaaacttg attttccacc tgtactttgc
    20521 ctgaatcgtg aaagcacagg tatgaaaatc aagttttttg ttgcgaaaaa acgtcctagt
    20581 caatcagttg gccgagcggc tggcggctga tctgctcctg atcaataacg agtgcttctt
    20641 ggaagtgttg ctgcaaaaag tagcggtcat ggctgactgc taggagcgtt ccttgatagt
    20701 cttgcagcat ttcttcaatt tcttctcgag catagctgtc gaggtgattg gttggttcat
    20761 ccaaaatcag gacgttgatt ttttctaaaa agagtttcag caacgccaaa cggatttttt
    20821 caccaccgga taaatctttg atccgtttac ccacatcgct ttgataaaag ccacttttgg
    20881 ctaattgccg tcgagcgact tcttcgtttc cagtcaatgt gatggcgtaa gccagcaaac
    20941 ggctatcggg atcggcaaac tgcagctgct gcggcaaata accgatttgc aagctactac
    21001 ctaatttgat ggtgccaccg taaggctttt cctcaccgag taataatttg agtaaggttg
    21061 attttcctgc accattgtcg ccgatgatgg ctaaatgatc tccacgatag actgcaaaac
    21121 tgctgtcttt gaatagtact tgatcagcgt actgctggtg caagttttga atgatgaaaa
    21181 cttctttgcc agattttcca ccattagaca agctttgcag ccgatttttc gggggaggtg
    21241 gcggtttgac gagtgtcagt ttcgccaggc ggcgttccag ttctttggct tttttgaaga
    21301 aggactcatt gtctccttca tgtgcccact gacgaaaacg acgaatcatg actttcaacc
    21361 gctggacttc tttttgctgc agttcgtaat tttttgtcag ctcagccaag cgagcttctt
    21421 tcaagactcg aaaacggctg tagtttccgg gatactcaat caatgcacct tcttccagtt
    21481 cccatgtttt tgtggtgact tgatcgagaa attgccgatc atgagaaacg accagaaccg
    21541 atttttttgt attctgtaaa tagttttcca accagcgaat actgttaaga tctaaatgat
    21601 tggtcggttc atcgagtaaa agtaaatcgg catcggctgc taaaattttg gctaaagcga
    21661 ctttaactcg ttcgcctccg ctaagttctg ttaaccgtgc ttcctgacag tgtggaatcc
    21721 ctaagccttg caagatcgct gcaatccggt cttctaatac gtaaccgcct agttcttcaa
    21781 agcgatgctg cagctcaccg taacgggcca gacacttctc taagtttgta tctggttctg
    21841 tcatcaatgc ttcgtagcga ctcatttgct gtttgaggtc ctgtatctca gcggcagaat
    21901 ggagcaagta gtggttgacg gtcgcttcgg aaactgtaag ttcttgttcg accgtcccga
    21961 tctttaagcc ttttttgcga ctgataacac cgctgtcgac accttctctg ccggtcaaga
    22021 tttgtaaaaa ggttgatttg ccagttccgt tgatatcgac taaggcaatt ttttcaccag
    22081 cttcaatttg acaggataaa tcctcaaata atggattccc gccgattgtt tttttaatag
    22141 aaattgcttg tatgatcatg atctctctcc ttgagaccac tcaaaaaggc catggttatc
    22201 cctggcctgt gttatttaaa ctcagattat catgagaaaa ataccttgag atggccattg
    22261 attttacgtg aattcaactg ttttggacaa acttgtcccg tcaacagcaa ctgtaaaacc
    22321 aattgcatgg ctaaatgcga tatggtatcg ttggtaattt tcattacttg ttattttagt
    22381 catgcgcatc atagtatttt ccctccttcg tgtagttgtc ttttccagtg tacctgaaag
    22441 tgtcagaaag ttcaactagc gctgacattt acttaatatt tatcgatgtg taattctgtc
    22501 agagagcttt ttttatctga ctgagggtaa tgtctcgtgg accacatcat tgtattctga
    22561 gctaaaccag cgttcaggcg aaatcatatg gtgtttgata aaaaactttg tctacatgac
    22621 ttccttttgc catttgctta ataccgttgc attaaacagc tcttctcttt ggaaatctca
    22681 ccaacatcgc gttcaggagt atcgttgtgg ttgcaatggc aagcggtagc ctttcatcaa
    22741 ttcttgaatg atttaccgaa tgtagcactt gccttctcgc ggtacccgaa cccgaaataa
    22801 aattttagtt tggcgataaa atcggtatcc gccatcgata cttcatattt tttcgtatca
    22861 gattcatcgg aaacttccac attgacgaac caataagctt tggcatgctt aggtcgtttt
    22921 aaaccacacc agggcgacta atcgccctgg tgttcacttt ttttatttta cttgaactca
    22981 ttgactgttt tccctttatt ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt
    23041 tgtttttatc acggtttttt gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt
    23101 gttaagcttt ggtgtggtct ttagctggca attggttttc taaatcagtt cggacaacaa
    23161 gcacatcgca agctgcgttt ctaataacat attcagaaac agaaccaata aataaacgtt
    23221 ctacagcatt caatcctgtg gctcctaaca taatcaaatc tacttgatta tcttctggga
    23281 tttctctggc aataatttgt tttggtgagc catattctac gacagaggtt acattattca
    23341 agccagcttt cttcgcattg ctttcataat ctgccaatgt ttgctttgcc atttcagtag
    23401 cttgttctgc aagcattcca tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga
    23461 catgtgctaa tagaagttct ccgttatttc ggatagctac attgaccgcc tttttaaaag
    23521 ctaattcagc ttcatcagaa ccatctacgg caaccatgat ttttttgtat tgttgtaaca
    23581 taagcattca ctcctcaagt caatcgtatt gtactctttt attgtagaac actttaggga
    23641 aaaagagaat tatttactgc tttggttcag caattttttt tgcaaatcgg atactgaagg
    23701 tggcagaaac aataattacg atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa
    23761 aagctgttaa aatacccatt acgcctaaaa cagcatatgc agcaccgtaa aattgtagga
    23821 atccttgatt tttatcattt ttgttgatca atacaaaaaa gatatcctgt ttattaaata
    23881 aataatagct aacgaataat aatactaaag tggcgataat cattaaaatc ctaatcatga
    23941 acaaatcctc caaaataagc aacaaataga aaaagcaatt tgccaagagc aaattgcttt
    24001 attctttaaa agatggtaat ctccgttaat gccgttgttt gtcctgtagt attgatcccg
    24061 cgaattcaag caggtgggtc ccatggtcgt agcttcgaac taccaaatga taaggcatgt
    24121 taccagcagt gacacaagac gggtcccaag atatcaataa aaatgttcgg tcaacacaca
    24181 aagaggacaa ttcgaacatc acagatttcc cacctctata gtagcatatg aatttaaaaa
    24241 gagcaatgag agagctcgca gaaacagaac cgttttcttg tctagttttt tggaggatgc
    24301 tttttccact cttttatacg gtaatactgt tgacccagag cctgttcata tttccctgta
    24361 tcttttggct gataatactg agcattcttc agtttatctg gtaaatattg ctgattgacc
    24421 caagcctgat caaaatgatg aggatactga taacccacgc cacggttgag tgatttcgca
    24481 cctttgtaat ggctatctct caaatgatca ggaacatctc cagctttgcc ctctcgaata
    24541 tcagcaagtg ctgcatctaa tgccatgtag gcagaattgg attttggcga caagcaaaga
    24601 tcgacgacga cgtctgctag tgggatacgt gcctcaggca atcccaattt ttcagcagct
    24661 aatacagcgt tcactgtacg agccgcagca gcaggattac ctaaacctat atcttcataa
    24721 ccgataacca tcagacgccg gcaaatactt gccagatcac ctgcctcaac taaacgggcg
    24781 agataatgaa gtgcagcatc cacatcactt ccacgaatcg atttttgaaa agccgaaata
    24841 acgtcataat gagcatctcc atttttgtca tgggtaagag cttttctttg gacacattct
    24901 tcaatgatcg acagtgtaag atgaatcctt ccttctttat ctgaaggagt cgatagtgta
    24961 gccagttcca gcccattcaa tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga
    25021 agaagagcat cttcgtctaa ctggattgct tgttgtccaa ggccgcgttc tttatccctt
    25081 aaagcatgtt cgactgccaa ctggatgtct tgttctgtta atggtttgac ttcaaagatc
    25141 tgtgtacggc tgcggatcgc tggattgatg gtaatataag gattctcagt agtcgctccg
    25201 atcaagatga ttcggccact ttctaaatga ggaagcagaa aatcctgttt tgttttatct
    25261 aatcggtgta cttcatccaa taagaggatc actgtaccac tcatttttgc ttcttccgct
    25321 acaacttgta gatccttttt tgaatctgta gcggcgttta gcatgcgaaa tgcataatta
    25381 gtagagccgg cgatcgcact tgcgatactc gtttttccag taccaggagg tccatataga
    25441 atcatggaag agagcatgcg agcatcgacc atgcggcgta tgatttttcc ttccccgact
    25501 agatgctgct gaccaacgac ttcatcgatc gttcgaggtc tcatgcgata agccaatggc
    25561 tgttgcatag gattttcctc cttcttgttt ttacgtccct taattatatc atatttagca
    25621 agatgcctag aaagaacagc cttctgttgt tttgttttca tagcttatcc aactaatatt
    25681 gtgtatgata aagtaggtga gaaaaatgac ggataaacaa aagatagatg aatttttttg
    25741 gaataaaacg acgccagaag tggcacggga tttattgggg atgtatttgg agcatgacac
    25801 accagaagga agattagctg gatatattgt agacgcggaa gcatacctag gtccagaaga
    25861 cgaggccgcc catagctatg gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa
    25921 accaggtacg atctaccttt acacgatgca tactcatcgg attttgaaca tcattactca
    25981 gccagaaggg attcctcaag gagtaatgat acgtgcaatc gaacccgccg caatgattga
    26041 tcaaatgagt aaaaatcgtg gcggaaaaac tggtccagat atcagcaacg gtccgggaaa
    26101 attagtggaa gcattagcaa ttcctcaaga actttatggt cagtctatag cagatagttc
    26161 gttacgctta gtttttgaga aaaaaaagac accaaaaaag atcaatgctt tgcctagaat
    26221 cggtatccca aacaaaggtg tatggacaga aaagccgttg cgttttgttg tgtcgggaaa
    26281 tccttatatc tctttacaaa gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga
    26341 aaatgaaaaa gaaggatcta ttaacatatt tagatgaaat aattgaaaaa caagtgacag
    26401 actatgatgt agctttagat tgggatacaa aaaatcatac gatcgagatc gttgttcgct
    26461 tgtttgcaga aaataaagca caatttgaga ttgatgatgc agaagggatc gtttcgcaag
    26521 aaccaattat cgaatttgaa gacggcgtac ttctttttaa tcctcagaaa tctgtttttg
    26581 atgaacgcaa ttatctagca gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag
    26641 tagctgattc tttagtggaa tatctaaatg aagtattagc tcaagggcaa agcgatttgc
    26701 ttgatttttt ggatgaagac gatgacgaag ctgtattcga gcttcactgg gataatcaac
    26761 aactagcaga attagtagaa gtaaaacagc aaaatgaagc agatacttat ttaccgtacc
    26821 caagttatta aggagtgaca gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg
    26881 aagcagtcga agcagttgct aatattttga tggaggcagg ggcgagtggt gtcgcgattg
    26941 aagattcttt agatgttgaa cattttcagt cggatcaata tggtgagatt ttagataaaa
    27001 atgattttac atcgctgaaa gaaggcgcac tagtcatggc ttattttccg gaaacgactt
    27061 tcttaccgga aatcttgcct tttatcaaag atcgtatcac acaattacct gagtttggtc
    27121 tagctattgg aaaaaataaa gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag
    27181 cctggaagaa atactatcat ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct
    27241 gggaaaacta ccagacagaa gatcctctag aaaaaattat cacactagat cctggtatgg
    27301 catttggcac aggcactcat cccacgactt ccttgacatt gacagcacta gaaacagtct
    27361 tgcgtggtgg tgaaacattg ctagatgtgg gaactgggtc gggcgtgcta agtattgcag
    27421 caaaatatct tggtgcaaaa gatgtctatg cctttgattt agacgaagta gccgttcgtt
    27481 cggcgaaaga aaatatggat atgaatgaaa tagccaaaga tgtccatgta tcagctaacg
    27541 atcttttgac aggaatcgag attgaaagtg atgtaatcgt tgcgaacatt ttagcagata
    27601 tcatcttatt gatgatccca gatgcttggc gtttactgaa gcaaacaggc acgctgatcg
    27661 tttcaggaat cattgaagag aagaagcaaa tggttctgga tgccatgtta gaacaagggt
    27721 ttatcgtgga tcaaattttc cagcaaaaag actggtatgc aattattttg aaaaaaccag
    27781 aggtggaata gatgcagcgg tattttttaa aagaatcata tgaatcaaaa gactattttg
    27841 ttttagaagg ggaaaactat caccacattg tacgggttat gaggatggaa cccgaggatg
    27901 aagttttttt ggcattcaat gatcaaatag ccattgttgc agaaatcacg gagattactg
    27961 aagaggctgt ttatctgaaa gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt
    28021 cgattaccat agcgagtggc tatcctaaag gggaaaagct ggattggatc gttcagaaat
    28081 ccactgaatt aggagcgcaa gcttttcttg gtttcccggc atcagcctct gtcgttaaat
    28141 gggatgcaaa aaaacgtatg aaacgacaac aacggcttga aaagattgct caagaggcag
    28201 cggaacaatc ccatcggcag atcgtgccat cagtagagtt attggaaacg aaacaagatt
    28261 tatttgagcg gctagatgat tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc
    28321 aaggcgaaaa atcaaatgca gccaaaattc ttagtgaaac aaaaccagat agtcgaatct
    28381 tagctgtctt tggaccagaa ggtggattat cccgcgatga agtcgattat tttgtagaac
    28441 acaaagggat actttgtgga ttaggaccaa gaatccttcg tacagaaaca gcacctcttt
    28501 atttattaag tgctgctagc tatcaatggg aattaatgaa tccttctttc agctaacgtt
    28561 cttcattaat aaaagagtat cgacagaaag aattgaaaaa atagtcaaca atttgtataa
    28621 tagaaacaat tatttataca atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg
    28681 aacatttccg taaaaaaacg tataactgca cggattttgg gacaattatc aaacagatat
    28741 ttgtcccgat tcccgtttta ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa
    28801 gaaattttaa cgggacccgg tgtcataaag ctcgttagtc aatacatggg tcccgaacat
    28861 gtagcatttg ttgagaaagc atgcgaatac gcgactgctg cacatgacgg acagtttaga
    28921 aaatcaggcg aaccctacat tattcatcct atccaagtcg caggtatatt agcagattta
    28981 aaaatggatc cccatacagt ggctacaggc ttcttacatg atgttgttga agatacagaa
    29041 atcactttag aagatctgag agaagaattt ggcgatgacg ttgctatgtt agtggacggc
    29101 gtaaccaaat tagggaaaat aaaatataaa tcccacgaag agcagctagc agaaaaccac
    29161 cgaaagatgc tgctagcaat ggctcaagat ttacgagtta tcatggtcaa attagctgat
    29221 agactacata acatgcgtac gttgaagcac ctgcgagaag ataagcaaag aaggatcgct
    29281 caggaaactt tagaaatcta tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa
    29341 tgggaattag aagatacagc acttcgttat ctaaatccaa aacagtatta ccgtatcgtc
    29401 cacttgatgc agacgaagag agaagaacgt gaaaaatacg taagtggtac tgttgaagat
    29461 attcgaatag cgacagaaga gctggggatt tttgcagaaa tctatggacg gccaaaacac
    29521 atttattcaa tctatcgtaa aatgaaagat cagaaaaagc agttcaacga aatttatgac
    29581 ttgctagcta ttcgagtgat cgtagattca atcaaggatt gttatgctgt attaggagca
    29641 atccacacaa aatggaaacc aatgcccggc agatttaagg attatattgc catgccaaaa
    29701 gctaatatgt accaatcttt gcatactacc gtgatcggtc cggcaggaaa tccggtagaa
    29761 attcaaatcc gaacacagga aatgcatgaa atcgctgaat tcggggttgc tgcacactgg
    29821 gcctataaag aaggaaaaaa tgaaaaagta gaaccagatg gtatgacgaa acaattaagc
    29881 tggttccatg agatactcga acttcaagac gaaagctatg atgcttctga atttatggaa
    29941 ggcgtaaaag gagatatctt tagtgataaa gtctacgtct tcacaccaaa aggagacgtt
    30001 actgagttac caaaaggatc cggaccattg gactttgcat acagtatcca tacagatatc
    30061 ggtaacaaaa ccactggtgc aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg
    30121 aaaaacggag atatcattga gatcatgact tctccaaatt catttggccc aagtcgcgac
    30181 tggttgaaat tagttgctac tagcaaagca agaaataaga tcaaacgttt cttcaaagcc
    30241 caagatcgag aagaaaatgt gatcaaaggc cacgaatccg tggtcaaatg tattacagat
    30301 ctaggattta cgcctaaaga tattttgacg aagaacaaac tgcaagaagc actcgatcgt
    30361 tttaattatc aaacagaaga tgatctctat gcagctgtag ggtatggaga agttagcccc
    30421 ttgacgatgg ccaatcgtct gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa
    30481 aagcaagaag cagaagaaat catgaatcag ccgaaaaaag aacctgacaa aatgaaagta
    30541 cgtcatgaag gtggcgttgt cattcaaggg gtagaaaact tactaattcg tatcagtcgc
    30601 tgctgtaatc cgattcctgg tgatgatatc gttggttata tcactaaagg cagagggata
    30661 tccattcatc gtcgagattg tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt
    30721 ttgattgaag tcgaatggga agatacatcg aatacacgaa aagagtatga tgcagatttg
    30781 gaaatttacg gctataatcg ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg
    30841 ctaacgaaaa atctcaacag cgttgaagca cggacgaata aagataaaat ggcgacgatc
    30901 catttgacgg ttggtatcca gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa
    30961 gcagtacctg atgtctacag tgtacgccgg acgaatggat aggaggacag atatgagagc
    31021 agttgttcaa cgggtaagta aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat
    31081 tggctacggc ctacttgttt tattaggcat tcatgaaacc gatacgcaaa aagatgtgga
    31141 ctatctggta aaaaaaattg cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa
    31201 tattagtgta gaagatgtgg gaggacaaat tttaagtatc tctcaattca cactgtttgc
    31261 ggatacgaaa aaaggaaatc gaccaagctt tgtggaagcc gcacgaccag aaacagctat
    31321 tcctttatac gaatcattca atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg
    31381 acaattcgga gcagatatgt cggtttctct cgtcaatgat ggacctgtaa cgatcattat
    31441 tgattctaaa aacaaataaa gtaatctggt tttcactagt aaaaactatg aaacacgtcg
    31501 cttaagttag actgataaac tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct
    31561 tttgtcagca gtcggaaaaa aagttaagct taaattatac aagttatttt ttataggagg
    31621 ttgctgaaga tgaaagaagt accagtagta gatatcaaaa tcacagattt aaaaaaattg
    31681 tatcaaaaag gatacaacat gttggaggag ttacgccatg aagcggacgc tcccgtcgtc
    31741 aaagcaaaga ttttcaataa agaagctatc acgatctacg gatcatcagc agcaaaagta
    31801 ttttatgatc caaggaattt caaacgaaaa ggtgctatgc caaaattagt attgaagaca
    31861 ctttttggtc aaggtggagt acagactttg gatggagctg ctcatcacca tcggaaaaat
    31921 atttttatgg atctaatgac accggaaaga atggaagatt atcatcgtat cttagataag
    31981 aatctgacac aagcacttga agcacagcat ggtcaatttg aactatttga tttaagtaag
    32041 atggttttct ttacatctat ttgcgagtgg gcaggtatca atttatcagc gatttctaaa
    32101 gatgaggtcg aaaaattagc agaatatcaa atctctatga tcagcggaac gtttacttct
    32161 ccgattgatc acataaaagg tgtcgagaat cgcaaaaaat cagaaaaatg ggcgcaaggc
    32221 ttgattgaag aagctcgaca aaatcctgtg gctggaaaag aaaatgtggc attatatgct
    32281 tttgccaatg ccacagattt agatggtcag cttttacctc tggaagtggc agctgttgaa
    32341 ttattgaaca tcatccgtcc aacggttgct ctaactgtgt gggcggcatt gatgggacat
    32401 gcacttttca gtagaccaga tttgtatcag caattaaaaa atgacttttc gacgttacaa
    32461 gatccgttta ttcaagaaat gagacgctat tatcctttct tcccaatgct acctgcgatt
    32521 tcgctgaaag aagtagaagt agatggttat cgtataccag aaggcagttg ggttatttta
    32581 gatttatatg gaacagacca tgatgaacga acagttgaag cgccagattc ctttatgatc
    32641 aaacggtatg tagggaaagc aaaagatatt tcctataaag aagaatatga gatgattgca
    32701 caaggcgggg gaaattttcg acaaatgcat cgctgtgcag gagaatggat cactcttcat
    32761 agtctacgtg tgttttccga tcagctagta aataagtttg agttctctgt acctgaacaa
    32821 gattggacga ttccattcaa tcagtttccg acttatccga atagccgagc gctactttac
    32881 aagaattaga ttagcataaa cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc
    32941 ttttttcata ttactataaa ttgataaggt caattgatca aaatccacga aatgcttcat
    33001 caatggtttg ataatcttca ttggataaat gcaaatctaa agcttggaca ttgtcagata
    33061 cttgctctgg acgcttagct ccaggtataa ctacagacac tcgtggattc ttcatgtacc
    33121 aagctaaaac aagttgcgca ggagtggctt ggtatctttt agcaatgggg cgaagtacat
    33181 cgacagcggt taaaatttct ctgaaacgtt ctccttggaa atctggattt ctttttcgag
    33241 gatctccttc accaaattgt ttttcctcac caagttcata ttttcctgtc agcaaaccgg
    33301 aagcaagagg aaaataggga acaaaactga ttttgttttt ttcaagataa gggaataatt
    33361 ctttttcagc ctgccgatgg atcaaactgt atttatcttc tactacatcg acatatccat
    33421 ctgcattcgc ttctttcaat tgttctaacg tgaagtttga cacaccgact gctcggattt
    33481 ttcctgcttc ttttaattcg tgcaatgtag caactgattc attttttggt gtgctttcat
    33541 ccggaaagtg gatataaaaa atgtcgatgt aatcagtttg cagacgtttt agtgcatctt
    33601 ccaccgattg tttgagaaac tcaggagaat tatcaaatgt tcgcccttta tttggtacat
    33661 gagcagcttt tgtagcgatg acgacacgtg agcgatcata ttctttttcc ttcagtacct
    33721 cgccaatcag ttcttcggaa cggccgtttc cgtaagcata agcggtatcg atcaaattga
    33781 tcccgctatt caaagctgta cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa
    33841 aaagattatg tcctccaacg gcatttgctc ctaatccaag ctgttctgcg tagacttgtg
    33901 aatgtccgat tcttatttct gccatagctg attcctcctt tttgtctagt ctaaaacaaa
    33961 ataccaaaaa gctgaactaa aacgtttttg tgaggggaaa tagtcagata tctcttgact
    34021 ttgagactgg tttttagtag gttataagta atatcgaatg tcaatgaaag agcgagtaag
    34081 tttttgaaac tttacaagag aggatcatcg aggctgaaaa tgatctaaag tttcattagc
    34141 ctaaagacac tctggagatg gacgagtgaa aagccgtccc gcgtacgagc gttattcgta
    34201 agaggaaaga gaaatctttc aaaattaggt ggtaccgtgt atgaatttca ttcacccttg
    34261 ttcatgatga acaagggtga tttttttatt tacatagcta agaaagggag agaaaactat
    34321 gagtttccaa agaccaaaag gaacaaatga tattttgcca ggcgaatcag aaaaatggca
    34381 gtttgtcgaa gaaacagcac gtttattatt cagagattat cagtataatg aaatgcgtac
    34441 accgattttt gaacactatg aagtgatatc ccgaagtgta ggggatacaa cggatatcgt
    34501 ttcaaaagag atgtatgatt tttatgacaa aggggaacgc catgtgacgc ttcgaccaga
    34561 aggtacagca ccaatcgttc gttcttttgt tgagcataaa ttgttcggcc cagagtatgc
    34621 gaaaccgttc aaaacttttt atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg
    34681 acggttacgt caattccatc aaatcggagt agaagcattt ggatcagaaa accctgcaac
    34741 tgatgtggag agcatggtga tggcacttga tttcttcaaa cagttgggga tcacgcaaat
    34801 ccggctagtc atcaattcat taggtgataa agaaacgcgt caagcctatc gtcaagcatt
    34861 gatcgactat cttttacctt ttgaatcaga gctgagtgaa gactctaaac gccgccttca
    34921 tgaaaatcca ttacgcgtat tggacagtaa agacaaacga gatcaaaaat ttgtggcaga
    34981 agctccatct atcttagatt atctaagcga gcctgctcaa aaacattttg ataccgttgt
    35041 ttccatgctg gatgcattgg ctgttcctta tgaaatcgac agtaatatgg ttcgcggact
    35101 agattattac acacatacga tttttgaaat catgagtgac gcaccaaaaa tgggggcaca
    35161 agcaacgatt tgcgcaggcg gacgttatga caatctagtg gaagaactag gtggtccttc
    35221 aacacctggc ttcggatttg caatgggtat cgagcgatta ctcttgacta tggaggcaga
    35281 ggaagtagtt atccctgcat ttaatgagtt ggatgcttac gtggtcgcac tcggagacga
    35341 aacaaatatc gaagcattga aagtggtcca agcgattcga aactttggtt tttcagctga
    35401 ccgcgacttc atgaaccgta aagcaaaagc gcaatttaag acagcagata aagcgaatgc
    35461 caaattagta ttggtgatcg gtgaagatga gcttgctagt caaacgatta atgtcaaagc
    35521 aatggcaagc cgaaaagaaa aaagttttcc attagaagag atatatgaac attttgatga
    35581 agtctatgat gaaatgactt tattcgagta ggaggaaaaa aataaatggc acaaagaacg
    35641 atctattgcg gattagtttc cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg
    35701 gtacaaaaaa gacgagacct tgggggagta atctttatcg acctacgtga tcgtgaagga
    35761 atcgttcaaa tcgtctttaa tcctgaaatc aacaaagaag catgggaaat cgcggataaa
    35821 tgtcgcagtg aatacgtgat cgaagtgact ggggaagtta gaagacgcgg agaaaatgca
    35881 gtaaatccta aaatggccac tggtgaattc gaagtgattg caagtgaaat cgtgattttg
    35941 aatcaagcaa aaaccccgcc atttcaaatt gaagatgatc aagcaatcag tgacgaaata
    36001 cgtttgaaat atcgttattt ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta
    36061 agaaatgcga caacaaaagc gattcgtcat tatttagatg ataatgactt tctcgatatc
    36121 gaaacacctt acctagggaa atctacacca gaaggcgcaa gagactacct agttccttca
    36181 cgtgtccatg ctggacattt ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg
    36241 ctgatgaatg ctggattcga ccgttattat caaatcgttc gttgtttccg cgatgaagat
    36301 ttacgtggag accgtcagcc tgaatttacg caagtagata ttgaaacaac gttcttaaca
    36361 gcagaagaaa tccaaactta cacagaggga ttgattgcga aggtaatgaa agaagttcgc
    36421 ggtatcgaag tcacattgcc atttcctcgt atgacctatg atgaagcaat ggctcgctac
    36481 ggcagcgaca aaccggatac acgttttgat atggaattga tcgatctaag cgatacagtc
    36541 aaagaagtag aattcaaagt cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca
    36601 ttgaatgcta aaggcgctgc agatcgctac tcacgaaaag acatggatca gttaggtcaa
    36661 tatgtaggac aattcggagc taaggggctt gcatggctga aagtagaaga agacggattg
    36721 aaaggtccaa ttgctaaatt catgggagaa gcaacagaag caatcattaa agcaacagat
    36781 gcaaaaccag gtgacttgct gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca
    36841 ttaggtgcta tccggacacg tttaggaaaa gaattaggac taattgatga atcgaaattc
    36901 aatttcttat gggtaactga ttggccacaa tttgaatttt cagaagaaga aggtcgatat
    36961 gtctctgctc accatccatt cacgatgcca aaagaagaag atatcccgtt attggctgaa
    37021 gaccctgcaa aagtctatgc agaagcttat gatatcgtct tgaacggtta tgaattaggc
    37081 ggaggttcta tccgtattca tactcgtgag cttcaagaaa aagtactaga aactctaggc
    37141 tttacaaaag aacaaatgga agaacaattt ggtttcttgt taacagcttt agattacgga
    37201 ttccctccac atggcggtat tgcattagga ttggaccgtt ttgcaatgtt attagccgga
    37261 gaagataata ttcgtgaagt cattgccttt cctaaaaacg gaaaagcagc agatgtgatg
    37321 agtgatgcac caagtatggt ttcgccttta caattgttcg aattgaatat tgatgtaacg
    37381 aatatagaag aatagccgat aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac
    37441 aaccttgatt taattgaaaa acactttata atgaagccat aacatataaa gcggggcttt
    37501 tccatgaaaa aaacattcga aaattgggct tatcatgatt acacgaccaa gctttcggta
    37561 tctatcgttt atgcgttgat cgcatcagtc gcattgaatt tcttttatca gccaggacat
    37621 atttattcaa gtggtattac tggtctagct cagattttga cgactttgtc aaaatcactg
    37681 ataggattcg aagtaccggt atcaacaact ttgtatttac tgaatatacc tttgtttgta
    37741 ttggcgtggt taaaaatcgg gaagaagttt acaattttca cttttatcac agtcactttg
    37801 acctcccttt gtatccatat tttaccggaa acagtcctat ctaccgatcc aatcatctgt
    37861 gcgttgtttg ggggggctgt gatgggacta ggtattgggc tagcgttgag aaacggccta
    37921 tcatcgggcg gtctagatat cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt
    37981 ggttctattt cgatctactt caatgcattg atcgtttttg ttgccggaat tctatttggc
    38041 tggcaatata tgttttacag tacgttgtct attttcgtca gtggaaaagt aactgatgtc
    38101 gtttatacta aacaaaagaa gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta
    38161 gtaaatgaaa tccaaaactg tatgcgtaga gggatcacga tcatcaatga agcagaaggt
    38221 gcttataagc atgataagca gacagtattg atgacgatcg ttacacgata cgaactgcct
    38281 ctacttagag aagcaatgaa agcttctgat ccacaagctt ttgtaagtat tgcagaaaac
    38341 gtccagatac ttggtcgttt ctatgaggat ggcatttaga tcgagtttga caaaaattca
    38401 tgaaatactg ctgtagacag acgttcgttt gtctgcagtt tttttctacc atagtagaat
    38461 cagcaaagaa aaactgcttt tttatttgaa attttcttaa agttgaaaaa aagcatgttt
    38521 atttttttaa atttatggga tgatagaaaa aaatagtaga gaaaggacac gattctactt
    38581 gctattttca cttaataccc gtatattacg aatgaaaaaa gtttatgaaa agttggaata
    38641 ttgaatggaa aaacaaaaaa ggttaaaaaa cagccgctac atcactgggt ttgatgggct
    38701 ccgttcatta gctgtggtgg gtgttatctt ctatcacctt ctgccaacta gtatgaaagg
    38761 cggctatttg ggcgttccga tttttttcgt cgtatctggt tatttgatca cggatctgct
    38821 gcggcaggaa tgggaacaaa atggaaaaat caatatttgg caattttatg tcagaagaat
    38881 gaagagactc tatccggcac ttgtttttat gcttgtgaca gcttcggctt atatcacttt
    38941 gtttcagcga gggctgttaa acaatcttcg aggagttgtg gtcagcagct tactgtatgt
    39001 caacaactgg tggcaaatca ataacggttt atcttatttc gatcgatttg ctaatgagtc
    39061 accgtttacg catatctggt ctttagcagt agaaggtcaa aactatttgg tttggccgat
    39121 cttgtttgta ttgttgatgg tatttgttcg taaaagaaaa tggatctttt atactgtgtt
    39181 aggtacatcc ttcctctccg ccatcttgat gatgatcttg tatacaccag gaggcgatcc
    39241 aacccgtgtt tattacggta ccgacactcg tctgttttct atatggttgg gaagtgcgct
    39301 tgcgtttgtt tggccaagta cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat
    39361 tctgaatatc gctggaggaa tctcgctttt ggcactgatt ctttgcttct tctttttaga
    39421 tgatcattac aatttcatct attacggagg aatgctccta gtgagtctgt tatgcgtagt
    39481 gctagttgca gtgacagcac accccggagc aagtttgaat cgatggctga caaatccagt
    39541 atttacttgg attggcaaac gaagctatgg catttatctg tatcagtttc cagttatgat
    39601 tttttatgaa gcaaaggtga ctgatattgc cgatcatgtt ttgttgcata ctttagtgga
    39661 aatcacattg attctaatca tcagtgaact ttcttatcga tatgtggagc gtccgctagc
    39721 acgttttgat tatggtcgta cattccaagt gatgaaagga tggttcagca aaccgatcat
    39781 cagtaagagc aaaccatggg ttgttccagg cacattactt gtagcaattg cactcgttgg
    39841 tttcgttacg gcaccgaaaa acgctgtaac agccgatcaa aaacaactgc aggcaaaaat
    39901 cgctgaaagt aaaaaattag cagaagaaac cagaaacaat tcagaaaaca ccactagttc
    39961 ggttgaccaa gcgatccttg ataagtacga gttgtccaag gcacaaggga ataaggcaca
    40021 aaaattagaa ctaacggcat ttggtgattc agttatgttg gatgctgcaa cggatctaca
    40081 agaactttat ccaaaagtag tggtagatgg agatgtcgga cgacaattat atgccagtgt
    40141 accgtacatt gaaaaattaa aggaacaaga tttgttgaaa gacactgttc tgattggact
    40201 aggaacgaat ggctcattct cggaagctca gtttgatagt gtgatggatg caatcggtga
    40261 tcgaaaagta tactgggtca atgtccgagt accgacgaag cgctggcaaa atgacgtgaa
    40321 tgcgatgctt gaacagatga cgaaaaaata cgacaatatg acgctgattg attggtacag
    40381 ctacagtaat gaccatagtg actggtttta tgatgatcaa gtccatccaa atcctgaagg
    40441 gatgacacat tacattcact tagtcagcga agccttgcta ggaggaagtt caagtacgaa
    40501 cagcagtgag acaaacgtga cagaatgata gctacaaaaa acttcaggca aaaacatttg
    40561 tttttgcctg aagttttttt atatttaccg tgtttttaac attcatatag attcaggaag
    40621 caaaacggtt gagtgtgtct gtaaataacg ttctttttgt tttcgggtga gttttttgaa
    40681 tgcagcttct gcttttgttg cttcgcttct tgaagaaaat acttctgcat ggatcatctt
    40741 aacaggtctt cttttttgaa gacgagtata tttagcacca gtaccattgt tatgttcctc
    40801 taatcgtcta gaaagatcgg ttgtatatcc accataaaat gtctgatctt tacagaataa
    40861 tacgtaaaaa taatgttcat ttgccataga gcatctcacg aatctctgca gtgtattctc
    40921 catccatatt gtaagtgatc aatggtggag cgactttgaa tccgtctaat ttgccttctt
    40981 tgattccttc aactaacaaa atatttgcct cttttccagg ttttgggtaa acaaattgga
    41041 tccttttagg tgcaattcga tagtcttcca tgacctgaat gatttctaag aaccgatcag
    41101 gacgatggac catagtaaag cgtccatttg tttttagtag cttacttgaa atatcgatca
    41161 cttcttctaa cgtcgtatga atctcatgac gggcgattgc caggtgttta ttagggttcg
    41221 ttttatttgt tggcagccct ttgaaataag gtggattaca gacgactaag tcacaagaat
    41281 tatgtcgaac gacagataag ctgtctttca gatcgatggt gtggacagtc atttgttcat
    41341 ttaaatggtt aagcgcgata ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga
    41401 tagcgtcgat agccgcctgg gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg
    41461 cacaaaggtc aacaatcttc ccttttttag gtacacggga aaagtgagca agcagcacag
    41521 catctaaaga aaaagaaaac actttggaac tttggataat cttgatatct tctgcgtata
    41581 attggtcgat tcgttcgtct tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc
    41641 gtctgtatta tactattttt attaaaaaga accaaggtgg cttgtcaaac ctctattttt
    41701 tatgcatact atgaccataa gaagggaaag gaagagacta tgttttatcg atttatgcgc
    41761 ggggtagttc gagtactatt agccttagtt aatggaaatg cccgctatga gaataaagaa
    41821 gttttaccgc aagatgagaa ttacatacta gttgctcccc atcgtacttg gtgggaccca
    41881 ttatatctgg cggtagctgc agcacccaag gaattcagct ttatggcaaa agaagaactg
    41941 ttcaaaaatc cagttctgcg atttatatta actcgatcca atgcttttcc agttaaaaga
    42001 gaaaatccag gaccaagcgt cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg
    42061 agtttgatca tgtttcccag tgggacaaga catgctacag aacttaaagg cggaatggca
    42121 ttgatttcca gaatgtctaa agcgaaaatc gtgccagctg tatatcaagg tccgttgaca
    42181 cttggggatc tgttcaagcg tcagagagtg acagtgcgtt tcggcgaacc aatcgactta
    42241 tcagatatca agaaaacgga taaagaaggt atggcggaga tcgaacgacg aacacaagca
    42301 gcttttgaca aattggacaa agaagtgaac cctgatttca aatatgaagt caaaaaaaaa
    42361 taaaatgata agtttatgcg tgggcggtct tttgaatcta ctgtcctgtt cagttcagac
    42421 tttttcaatg aaaacgaggg agagacgtca agtctgccct ctttttgcgt tccaatatgt
    42481 aaaatagaag acaggtgtca tgtaatggat caaagtgtta taataattag aaggacattt
    42541 tttgaggtga ttgaatgcgt tttttacata cagccgattg gcatatcgga aaaaaactac
    42601 aaggttatga cttgattgaa gaccagcgtc atgtactatc ttctatacta gatatcgcga
    42661 aagaagaaaa agtagaggca atcgtgattg ccggagactt atatgatagg agtgtcccag
    42721 ctgttgatgc cgtagaatta tacaatgaat tgatggtcga ttggaattta aaggaaaaga
    42781 tcccgatttt tgctatatca ggaaatcacg atagcagtac gagactttca gcgggtacgc
    42841 catggttcag ttattcgaac tattttttgc gaacacagct agcagaagca tttgaaccag
    42901 tcgagtttaa aaacgtacaa tttttctttc ttccttactt tgaaccaatt gcagcgagat
    42961 tatattttga agatgacaat atccgaacga tcaagcaagc ggtagccaag gtgatagata
    43021 aaatgcagga gctatttgat tcagaaaaaa aacaagtatt gattagccat ttttttgtaa
    43081 tgggaagtgt aaaatctgat tcggaaacga aaatcgaagt aggcgggttg gattcgatcc
    43141 ctggtgaact acttacaccg tttgattatg tagcactagg acatttgcat aatcaagctg
    43201 ctttgagaca ggataatgct cgctatagcg gttccccatt gaaattctct ttatctgaga
    43261 tcaaccaaaa aaaaggcgta tggatcgttg acctgacaac aggcctggag ttgaatttta
    43321 gggagatcga acccctgcat gatattgaag aaataacagc gagtttcaaa gaattgctgg
    43381 atcctgcctt ttatcagtcc gtcaatcggg agaattacat ctatatctat ctcactgata
    43441 gaacgatcat cccgaatatg atgaaccagc ttcggcaaat atatcctcgt attttgggga
    43501 ttgaacgggc aaacggtcga gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga
    43561 aagatcctca aaaattagca gaagcatttt ttagtgaaat gacggaagag cagctaactg
    43621 caaaccaaga aaaatggctc ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa
    43681 gaatgaaacc gaaaaaatta caacttaaaa attttggtcc ttttatcaat gagaccgtag
    43741 atttttcacg tttgacagaa gcaccgcttt ttttgatcag cgggaagaca ggagctggca
    43801 aaacgacgat ttttgatggg atcaccttcg cactttttgg tgaaacttcc ggaagacttc
    43861 gctctggaaa agagatgcgt tctttgtttg ctacaccaga cgaagaaaca agcgtgactt
    43921 tttcttttga acatcagcag atgagctatg aaatcgaacg aaaaccagaa caagttttag
    43981 caaaacgaaa aggaaacgga acaaaaaaac aggcagccaa agtaacgttg accatttttg
    44041 atgggaaagg aaaagaaata aaacaaatca caaaacgtac agaagcagat cagttgatca
    44101 gagaactgat gaatctcgat gcgaaacagt tttctcaaat cgttttacta ccccaaggag
    44161 aattccgtaa ttttttggtt tcatcaagca gtgataaaga gactgtttta cgtcatttat
    44221 tcggtactca atttttccaa cagttcaatg agcgattaaa agaaaaagca aaacaacagc
    44281 aacaaaaatt ggatcattta gagcaagagc tgcatcttct gcaaaaaagg tttgttttag
    44341 tcaaagaaga ggaaccagca gcagggtttg aagccgtttt agcccaatgg acaaaccatc
    44401 aggcaatact cgaaaaacaa atcacggaag aaaaagaagc tctaacggaa cttcaggaaa
    44461 aacaaaagca actagaaaat tcctactatc gttttgaaag cttgaaaaaa agttatgaag
    44521 aaaaaacgat attaataaac aagcaaaagg aattagctgc tcaaaaagac gaaatagagg
    44581 aaaagaaacg atggattggg tattatgaat ttacgcaacg attagtcgaa ccaatccagc
    44641 gcctacaaga aataaaagtt gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa
    44701 agaaactagc gatgactacg cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg
    44761 tacgccgaca aacaaaaatc aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg
    44821 tgccaattgt cgaatcctta cgtagtaaaa aacaagacgc agaacaatta acaatttcat
    44881 tggataaaga agaattgaat gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa
    44941 gaactcgaat tcttcaagaa aatttactgg atcaggaaaa actgcaagaa gatcgtcttg
    45001 cttttggaca gttaaaatat ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa
    45061 aagacactgg gtcgctcgca cagataagag aagaactcaa aaaagcaact gaagaaataa
    45121 caaaaaatag tcagctattg aaagaacaga cagaacaagc agaacagctg aaaagccaat
    45181 gggcaaagca gcaaattgct cgtcttcagc tttttttgcg tccaggggag ccatgtcttg
    45241 tttgtggatc aacggaacac cccatacaac aattaaaaca cgaacagcca aataaggaag
    45301 atatcttaaa agtagaacaa caactggcag aagctgagtc tgctgtagaa agaactcagc
    45361 aaatcattgc caaaagtgaa gcacatgaaa ataatcttca acaacaatta tcagatttag
    45421 aacaaaaaat catggcaacg aatatacaat tggctgaaca agaagaacac ttgcaacagg
    45481 ctttgaaaaa ggcatttcct gagataacag aacaaaaaac aaaaacaact cttgtagtaa
    45541 tcgaacaatc tttagataga cgaaaaagca agatggccaa tgaaagaata gagctggaag
    45601 aaataagaca acaagcagaa aagctcgaac aacaattagc tgtctacaaa cagcagcaga
    45661 cagaaaaaaa taatcagctc cagcaactgc aaggagaaat caaaagttta gaacaacaat
    45721 tgcaagatac ggatagtaac tgtttagaag aggagatacg acgacttgaa aaacagttag
    45781 aagaagactc gaatcaacta gctgttgagc agaaagcagg agtgcaattg ttgcaagatc
    45841 ttcatacgct tcggacacag cttgatcttc aacatgaaca aatagaaaaa atagatgaaa
    45901 aaatagaaaa ggaacaagaa agaatagcta aaaagttaga aagccagtcg tatttttcaa
    45961 caattgaaga aatctgtgca tttgcacaac agcaagcaca atataaagaa aaccaacgta
    46021 cagttacttc ttatcatgaa gagcaattag tgacagctga ccggctgcgt caattagaaa
    46081 atatccagct aactgagaca ttcccagata cagaagagat cagccaacag ttagaagaaa
    46141 taagacgtat gatcgaaata aagcaagaaa agatatacgg tttacaagaa caaaaaatta
    46201 gtaatcaaaa aatctacgat gaatgtcttt cgatttatca cacaagccag acgaaattag
    46261 atgagcttac gcaattacag cagctttcgc aaacattcaa cggagaaaat cctaagaaga
    46321 ctagtttgga acggtatgtg cttcaggttt atcttcaaga agtgttacaa gtagcaaatg
    46381 atcatctcca acgtttaacg aaaaatcgtt atcaatttga gctagcggat accattggca
    46441 gttatcgagg gaaaaccgga ttagaaatca atatctatga tgatcaggca ggtatgtctc
    46501 gaagtgcgca taccctgtct ggcggtgaga gttttattgc agcactttct ttagcattag
    46561 ctcttgccga tgtgatccag acacaagctg gtggtgtagc aatcgatgca ttgtttattg
    46621 atgaaggatt tgggtcatta gatgaagaag ctttggaaat ggcaatggaa gctttagaaa
    46681 cgatcgaaag tgaaggaaga atgatcggca tcattagtca tgtaagagaa ttgaaagaac
    46741 gagtattaca acaaataaga attgatacag aaggtagcgg acaaagcaaa atacgctatc
    46801 aacttggata aaaagggaga ttggtaacta tgaaatggag tattccagaa aaagtcattg
    46861 cacgtggacg tcaatacttg caagaaggcc gagtgttatc ggttgtccct gaccaagaag
    46921 aaaacgtgtg gcatgcagaa gtcttgggca gtgaattgta tatggttgat ttggatggta
    46981 cagcaaaaga gatagattac tgccaatgtc cctattggga ggaacataaa tattgtaagc
    47041 atacagttgc tgtggaactt tatttacggt cgaaaaatct atctcgtatc atgaaaaaag
    47101 accagccaat gctagaaccg gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt
    47161 tttctcgtct aaaaacacaa aacaatcctc aagcggtgca acctttgatt attgaatacc
    47221 aagtagaaac gattgagact aatcagtatc acccagagct ttctattttg gcactttact
    47281 tgcgtatcgg ttcattggca tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt
    47341 ttttacaagc ttacacagaa aaagaaactt acactgttaa taaacagtac cagtttcgtc
    47401 tagataaacg ggcgtttcaa aaagaagatc aaaaaatact cacgcatctt gcaggaagtg
    47461 ctcagacgca tcaattatta ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa
    47521 gatatctgct tctcccagta gaacaagccc gcttcctgtt agagcagatg aatcagacta
    47581 cacgtttgca tctgcagata aatgaacaaa acgatcaagg aatacaattt tctgatcaaa
    47641 acaaaccttt atcattcaaa gtggaaaaat ttggagaaga ttatttgctg caagcattga
    47701 atgattttga ttttttcttt atgcattatc agtggggagt tatccaaggg acattttatt
    47761 ctctgacgaa atatcaacaa acggtttatc aaacttggaa acaattgatt cgccgattgg
    47821 agaaaccaga agtagtgttc aagaaaaaag aattaccgtc tttgtttaaa gagattatcc
    47881 ctctgctgtc agagatcggc gacgtagcta ttgcccctgc agtagaagag gaagtagcag
    47941 atatccccgt cgaatttatt ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag
    48001 tcgatttcgt ctatggtgaa gtcatctatt cgactgaccc gaagcatgag gtctcgacag
    48061 aacaggcttt tcaggtgtta cgtgacacag tagaagagaa acgagtactt gatctattca
    48121 gaatgtatcg ctaccagaaa aatgaaactg gttatgaacg tccattgcca agcggggaag
    48181 aactatatgc ctttttccgc acagaactat ctgtcttccg tcagtttggt gaagtcagac
    48241 ttggaagaaa attgcgagag ttgtatttag atgcgcataa acatcaaccg aaaatcgaag
    48301 taaccgagac cggttcatgg ctcgatattc gattcgatgt gacagggata gaggagcaag
    48361 aaatcgatca tgtcctgcaa agcttgctga gaaatgatgc tttttataca ttagaaaatg
    48421 gtcaggttct ttcttttgat tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat
    48481 tacgggaatc gatacgtaca gaagaaggaa cgatccatgt acctaaaaat caaggattga
    48541 tcattcaaaa tcaattggaa aaaagcaatg ctacttttag tgaatcgttc caaacgatgg
    48601 tacaagatct cattcatcca gaaagatacc aggcacagct tcccaaagga ttgaatgcaa
    48661 cgatgagaga ttatcaaaag caaggttttc gatggttgaa gatgttaggt cattatcagt
    48721 ttggcggtat tttagccgat gaaatgggac ttgggaaaac actacagaca attgcctttt
    48781 tactttcgga aaaagaagaa cggaaaagct tctctgcttt gatcgtagcg cctgcgagct
    48841 taatttacaa ctggcaggca gaggtgagaa aattcgcccc ttctttaagt atccaagtaa
    48901 tcaatgggaa taaaaaagaa cgtgaagaat tactggctaa agacacagat atccgagtga
    48961 cttcttatgc cagcttaaga caggatctag ctaattatca gtcgcaaaaa attgattatt
    49021 tgattcttga tgaggcacaa atggtcaaaa atagcagtac gaaaactgca caagcattga
    49081 gagaattagc tgtacctcag cggtttgcac tgagtgggac accaatcgag aataatttag
    49141 aagaattgtg gtcattgttt gcaacgatta tgccaggatt tttcccgact aaaacaaaat
    49201 tcagggaact tgccgcagaa gaaattgcgc aaatgattcg accgtttatt ttacggcgag
    49261 ataaacaaac tgtgttgaaa gacttgccgg aaaaaacaga gatgaatcta tattctgcac
    49321 ttacagaaga acaaaaaact gtttatttag cttacttgcg tcaaatgcgt gaagaaatca
    49381 catcaatgga ttctgaagca ttcaagaaaa accggatcgg gatacttgct ggtctgacaa
    49441 gactgcgaca aatttgctgc gatcctcgtc tgtttattga agattaccaa ggcggttccg
    49501 gcaagctcga acaagtaaaa gatctactgg tagctgccaa agagaacaaa cgaagagttt
    49561 tgctgttttc tcaatttaca gggatgctga ctatcttgca agaagagtta gccgagctag
    49621 ggatctcgac attttattta agaggcagta cgaaaccaca agatcggtta agtatggtag
    49681 acgcatttaa tgctggcgaa aaagatgttt ttctgatttc cttaaaggct gggggaactg
    49741 gattgaatct cacgggagca gatacggtca tcttgtatga cctttggtgg aatcctgcca
    49801 ttgaagaaca agctgctgga cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt
    49861 ggcgtatgat ctctgaagga acgattgagg aacgcatgga ttcactccag caagaaaaac
    49921 gcgaactgtt ccaaaaagtg atccaaggta atgaagaaca gttgacgaaa ctgactgaag
    49981 aagacatccg catgatttta agtgtaggag aaatcgagga gtaagagact cgaaaaagcg
    50041 ttcagtaatg ggtaagaaag cctttctaaa gaagcgattg tttatttgat ttaaaaaata
    50101 gcaaggaggc tgggattcca ttttatccca gcttccttgc tatcattttt ttatttctat
    50161 ttttaaaggt tatacaaaga ttgtcttcat tgtctctaac agctgatcaa aagaagaaat
    50221 ctcgatatcg caaattggag tctgttcaat tttgcgttct cgatggttga accaaagcgc
    50281 ttgccagtta gcactttttg cacctagaac atcattgtcg taattatcgc caacgtataa
    50341 ggtctcttct ggaagcatat gaaattcttt ctcagccagc tgaaaaatct ctttttctgg
    50401 tttttggaaa cctgttgctt gagaaatgag catattgcgg gaaggaatcc aattgttcaa
    50461 ttgaagttgc ttcagttttt ttgtctgatg gtcagtcggc ccatttgtga taattcctaa
    50521 tgggacattc ttttccttca agaaatcgag ggtttttttg acctcctcat gcaagcagat
    50581 attatcaagt tcttcctcgt aaattttttg aaataagagg ccatcttctt ccgtaatatg
    50641 agggtagtct aaatctttta gtgattggct gatccgatgt gctcgcatat attctagtgt
    50701 ccaatctcct gccattactt ttgggaagtt ttcatcacta tgatggcgga accggatata
    50761 tagtggatgc atatctgcat ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa
    50821 tggctgctgc tgatcataca tcgtatcatc aacatcaaac acaactgatt taatcaaaat
    50881 tttacacgcc tttcggtttt cattgatttt ttcacaaatc cttgactatt gtaacagaaa
    50941 aaattgtcta aaaaagctga taaataaagg tttttcttac tatatttcta tttccttgac
    51001 agaataaggt ttttatattt cagttttttt cactaaaaat cgtgaaacac gtcaataagt
    51061 ttgatgaaca aattaatcat tatggtagaa gtcgattgat ttattttaat gcaatctctg
    51121 tggaattttc gaaagattta tttttttaat acagtctcaa tataactatt caaacataaa
    51181 ataacgttat ttgatttatt tgtttatagt aaattttatt tagtaatcat tgctttaaaa
    51241 tactacgctt atataacaga gaaaataagc gttataataa agaacagtat tatttaaaat
    51301 gacagaaaac attactatta aagaaataaa aaacataaaa aatcttaaga aatcggtttc
    51361 atttagcttt tgagataaaa taatagtaca gttttatacg ctgtttcata tgtttagaaa
    51421 atttgaatta gatttgaaaa ctctaatttt taaatgataa aaaaagcgag aatgctggtt
    51481 ttactgtata ttaaccttag ataactttca tttttttaaa aaaattatga aaaaaaggta
    51541 gctatttaaa agttgattat gtataataga aacagatggg agattattat gatcttctgt
    51601 tacaaaaaag tgattcttat cacgaaaagg agtggtgcac gttgttaaca ttatatactt
    51661 ccccaagttg tacttcctgc agaaaggctc gtgcgtggtt gcaagaacat cagattccat
    51721 ttgttgaacg taatattttt tcagaaccat tgaacagttc tgaattaaaa gcgattttac
    51781 aaatgacaga agacggaaca gaagaaatca tctcgacacg ttccaaagta ttccaaaaac
    51841 tgaacatgga tttagatgat ctgccgttac aagaattatt ggaacttgtg caaaacaatc
    51901 ctggattgct tcgccgtcca atcatgattg atgataaacg tctgcaagtt ggttttaatg
    51961 aagatgaaat tcgtcggttc ctaccgcgcg atgtacgaca attagaatta cgtcaagcac
    52021 aattgatggc tggattatag aaggataata cgaaacttgg aaaaga
//
LOCUS       DACDXE010000013        51635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.013, whole genome shotgun sequence.
ACCESSION   DACDXE010000013 DACDXE010000000
VERSION     DACDXE010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.013"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            120..1046
                     /gene="cysK"
                     /locus_tag="IWM23_000756"
     CDS             120..1046
                     /gene="cysK"
                     /locus_tag="IWM23_000756"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAP9883422.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            1282..1728
                     /locus_tag="IWM23_000757"
     CDS             1282..1728
                     /locus_tag="IWM23_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9883423.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2001..3353
                     /locus_tag="IWM23_000758"
     CDS             2001..3353
                     /locus_tag="IWM23_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAP9883424.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            3524..3811
                     /locus_tag="IWM23_000759"
     CDS             3524..3811
                     /locus_tag="IWM23_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883425.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            4037..4480
                     /locus_tag="IWM23_000760"
     CDS             4037..4480
                     /locus_tag="IWM23_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9883426.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4486..5670)
                     /locus_tag="IWM23_000761"
     CDS             complement(4486..5670)
                     /locus_tag="IWM23_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAP9883427.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            5938..6636
                     /locus_tag="IWM23_000762"
     CDS             5938..6636
                     /locus_tag="IWM23_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAP9883428.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            6643..7320
                     /gene="nth"
                     /locus_tag="IWM23_000763"
     CDS             6643..7320
                     /gene="nth"
                     /locus_tag="IWM23_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAP9883429.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(7446..7979)
                     /locus_tag="IWM23_000764"
     CDS             complement(7446..7979)
                     /locus_tag="IWM23_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883430.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(8276..10657)
                     /locus_tag="IWM23_000765"
     CDS             complement(8276..10657)
                     /locus_tag="IWM23_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9883431.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(10647..11294)
                     /gene="recU"
                     /locus_tag="IWM23_000766"
     CDS             complement(10647..11294)
                     /gene="recU"
                     /locus_tag="IWM23_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAP9883432.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            11358..11897
                     /locus_tag="IWM23_000767"
     CDS             11358..11897
                     /locus_tag="IWM23_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAP9883433.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            11987..12406
                     /gene="gpsB"
                     /locus_tag="IWM23_000768"
     CDS             11987..12406
                     /gene="gpsB"
                     /locus_tag="IWM23_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAP9883434.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            12428..12795
                     /gene="rnpB"
                     /locus_tag="IWM23_000769"
     ncRNA           12428..12795
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IWM23_000769"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            13004..14170
                     /locus_tag="IWM23_000770"
     CDS             13004..14170
                     /locus_tag="IWM23_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAP9883435.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            14216..15712
                     /locus_tag="IWM23_000771"
     CDS             14216..15712
                     /locus_tag="IWM23_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAP9883436.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            15793..16476
                     /locus_tag="IWM23_000772"
     CDS             15793..16476
                     /locus_tag="IWM23_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883437.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            17121..18311
                     /locus_tag="IWM23_000773"
     CDS             17121..18311
                     /locus_tag="IWM23_000773"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAP9883438.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            18413..19366
                     /locus_tag="IWM23_000774"
     CDS             18413..19366
                     /locus_tag="IWM23_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAP9883439.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            19435..20784
                     /locus_tag="IWM23_000775"
     CDS             19435..20784
                     /locus_tag="IWM23_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883440.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(20967..21290)
                     /locus_tag="IWM23_000776"
     CDS             complement(20967..21290)
                     /locus_tag="IWM23_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883441.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            21419..22582
                     /locus_tag="IWM23_000777"
     CDS             21419..22582
                     /locus_tag="IWM23_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883442.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            22652..24001
                     /locus_tag="IWM23_000778"
     CDS             22652..24001
                     /locus_tag="IWM23_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9883443.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(24046..24477)
                     /locus_tag="IWM23_000779"
     CDS             complement(24046..24477)
                     /locus_tag="IWM23_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883444.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            24865..27507
                     /gene="alaS"
                     /locus_tag="IWM23_000780"
     CDS             24865..27507
                     /gene="alaS"
                     /locus_tag="IWM23_000780"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAP9883445.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27707..28303
                     /locus_tag="IWM23_000781"
     CDS             27707..28303
                     /locus_tag="IWM23_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9883446.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            28425..29573
                     /locus_tag="IWM23_000782"
     CDS             28425..29573
                     /locus_tag="IWM23_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9883447.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(29611..30024)
                     /locus_tag="IWM23_000783"
     CDS             complement(29611..30024)
                     /locus_tag="IWM23_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAP9883448.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            30103..30534
                     /locus_tag="IWM23_000784"
     CDS             30103..30534
                     /locus_tag="IWM23_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAP9883449.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(30633..30866)
                     /locus_tag="IWM23_000785"
     CDS             complement(30633..30866)
                     /locus_tag="IWM23_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAP9883450.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            31049..32719
                     /locus_tag="IWM23_000786"
     CDS             31049..32719
                     /locus_tag="IWM23_000786"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAP9883451.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            32866..33252
                     /locus_tag="IWM23_000787"
     CDS             32866..33252
                     /locus_tag="IWM23_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883452.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            33239..33937
                     /locus_tag="IWM23_000788"
     CDS             33239..33937
                     /locus_tag="IWM23_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9883453.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            33942..34406
                     /locus_tag="IWM23_000789"
     CDS             33942..34406
                     /locus_tag="IWM23_000789"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAP9883454.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            34403..35314
                     /locus_tag="IWM23_000790"
     CDS             34403..35314
                     /locus_tag="IWM23_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9883455.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            35549..36088
                     /gene="pyrR"
                     /locus_tag="IWM23_000791"
     CDS             35549..36088
                     /gene="pyrR"
                     /locus_tag="IWM23_000791"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAP9883456.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            36102..37385
                     /locus_tag="IWM23_000792"
     CDS             36102..37385
                     /locus_tag="IWM23_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAP9883457.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            37398..38324
                     /locus_tag="IWM23_000793"
     CDS             37398..38324
                     /locus_tag="IWM23_000793"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAP9883458.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            38343..39623
                     /locus_tag="IWM23_000794"
     CDS             38343..39623
                     /locus_tag="IWM23_000794"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAP9883459.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            39628..40704
                     /gene="carA"
                     /locus_tag="IWM23_000795"
     CDS             39628..40704
                     /gene="carA"
                     /locus_tag="IWM23_000795"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAP9883460.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            40709..43894
                     /gene="carB"
                     /locus_tag="IWM23_000796"
     CDS             40709..43894
                     /gene="carB"
                     /locus_tag="IWM23_000796"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAP9883461.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            43948..44724
                     /locus_tag="IWM23_000797"
     CDS             43948..44724
                     /locus_tag="IWM23_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAP9883462.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            44721..45653
                     /locus_tag="IWM23_000798"
     CDS             44721..45653
                     /locus_tag="IWM23_000798"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAP9883463.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            45650..46366
                     /gene="pyrF"
                     /locus_tag="IWM23_000799"
     CDS             45650..46366
                     /gene="pyrF"
                     /locus_tag="IWM23_000799"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAP9883464.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            46373..47062
                     /locus_tag="IWM23_000800"
     CDS             46373..47062
                     /locus_tag="IWM23_000800"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAP9883465.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            47035..47304
                     /locus_tag="IWM23_000801"
     CDS             47035..47304
                     /locus_tag="IWM23_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883466.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            47342..47983
                     /locus_tag="IWM23_000802"
     CDS             47342..47983
                     /locus_tag="IWM23_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883467.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            48014..48718
                     /locus_tag="IWM23_000803"
     CDS             48014..48718
                     /locus_tag="IWM23_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAP9883468.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            48715..49617
                     /locus_tag="IWM23_000804"
     CDS             48715..49617
                     /locus_tag="IWM23_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9883469.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(49775..51481)
                     /locus_tag="IWM23_000805"
     CDS             complement(49775..51481)
                     /locus_tag="IWM23_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAP9883470.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        16895 a         8983 c        11096 g        14661 t
ORIGIN      
        1 aattaccata tatggtatat ctttgtcttt tttttgctag tcaaagacga atcactcgcc
       61 ataaatgaac agattgtatt atgataagta agaagagcaa agacaaggag gaattgaaga
      121 tgacagctat ctataactca gtcaccgaat tgattggcaa aacgccaatc gtcaaattaa
      181 ataaaatcgt accagaagat tcagcagatg tttttgtaaa gcttgagttt tttaatccag
      241 gaggaagtgt aaaagatcgt attgctttaa gtatgataga aaaagcagaa cacgatggtc
      301 tactgaaacc aggtgatact atcattgaac ctacttctgg aaatacagga atcggattgt
      361 cgatggtagg agttgcaaaa ggatataaag taatcatcgt gatgcctgag acgatgagta
      421 tcgaaagacg tttattgatg aaagggtacg gcgcagagtt gattttgact cctggtgcgg
      481 atggtatttc tggatcaatc agagaagcag aacgtttggc aaaagaaaac ggctactttt
      541 taccattaca atttgaaaat gaagcaaatc ctcttgtgca tgagaagacg actggtcctg
      601 agatacatca agcatttgga gtaaatggct tggacgcgtt tgttgcaggt atcggtacag
      661 gaggaacgat cactggcgct ggccgagaat taaaacgtgt gtatccgaaa atcgaattaa
      721 tcggggtaga accagcagaa tctgctattt tagaagggaa agaagcggga cctcacaaaa
      781 tccaaggaat cggtacaggt tttgttccta aaacactgga tacttctgtg tatgataaag
      841 tcctttctat tagtggagac gaagcgatgg aaacagctag agaagttggg cgaaaagaag
      901 ggattttagt aggcatctca tctggcgctg caattgctgc cgcgctaaaa gtcgcaaaag
      961 aattaggaaa aggcaagaag gtattagctg tggttcccga caatggtgaa cgttatttat
     1021 ctacagccct ttaccaagag atataaggca cttggttttt ctccagatag gtttatgtaa
     1081 aaaactttta aagatgaaaa gaacacacaa aaatttatga tgagaaaaat tatttgaata
     1141 gagacgtaga atagtgaacg cgactgtttt acgtcttttt tcattaaaat acaataaaat
     1201 tgaattttct tttcatgtta agtggatttt aaaatgtaaa aatagtatga taagacgaga
     1261 ataacaacgt ggggtgttta catggaaaat aagttagtcg aagaggttat cgaaaagtta
     1321 aaaaaagaga acattcgaat cacacctcaa cggtacgcgg ttttagaata tttgattgaa
     1381 catcattccc atccaacggc tgatgagatt tataaagcac ttgaacatcg gtttccaaat
     1441 atgagtgttg cgactgttta caataacctt cgcttattta cttcgattgg atttgttcaa
     1501 gaaatgaagt acggcgatgc ttctagccga tttgatttca gttcaaagcg tcattatcat
     1561 gtaatttgtg aaaactgcgg taagattgtt gattttcatt acccagggct tgaagatata
     1621 gaaatggctg caagcagact gactgatttt gagattaatg aacatcggtt ggaattgtat
     1681 ggactctgtc cggattgtaa agagtcagca aaaaaggcgc aaatgtaaaa acagtttcta
     1741 caagtgttat acctaaaaaa agctagctgt gtaaaaaaaa cgctagcttt tttatttttt
     1801 tgtcttttat tcacaatctt agatataaaa gtgaatatta ttaaaataag aatcttgtag
     1861 atattgggtt cttatgcttg aaaaggaatg aaactacaaa caaacctttg tgaattcaca
     1921 agcattaaac tgttgtatcc gttttatttc tgttatagta gttttgtcga aggacaaaat
     1981 gttaatcgga gggaccataa atgaaagtag tagtaattgg ctgtactcat gcaggtactg
     2041 cagctgtaaa aagtatttta aaaaatcatc cagaagcaga agtgacagta tatgaaagaa
     2101 atgacaatat ttctttctta tcttgcggta tcgcacttta tgtcggcgga gtagtaaagg
     2161 acccagcagg cttgttctac tcaaatcctg aagaattatc ttcattagga gcaacagtta
     2221 aaatggaaca cgatgtaaca gacgtcgata cagaaaataa aacagtaacg gcaaaagatc
     2281 tgaaaacagg agaaacagaa acggtttctt atgataaatt agtgatgaca actggttcat
     2341 ggcctattat cccaccgatt agtggaatcg atgcgaaaaa tgtactccta tgcaaaaact
     2401 acaatcaagc aaatgaaatt attgcacaag cgaaagaagc aaaacgtgta gtaattgttg
     2461 gtggtggata tatcggtatt gaattagtag aagcttttgt cgaatctgga aaacaagtga
     2521 cattgattga tggattagat cgcatcttga ataaatatct agataaacca ttcacagata
     2581 tcttagaaaa agaattgaca gaccgcggtg ttactcttgc gctaggcgaa aatgttactg
     2641 aatttatcac agatgaagaa ggaaaagtga aacaagtagc tacaccaaca gatacatttg
     2701 atacagatat ggtcatttta tgtgtgggct tccgtccaaa tacaaaactg atcgaaggaa
     2761 aagtagatac actgccaaac ggggcaatca aagtgaatga atacatgcag acaagtaatc
     2821 cagacatttt tgcagctgga gactctgcag tagtcaacta caatccaagt ggcacgcaaa
     2881 actatattcc tttggcaaca aatgccgttc gtcaaggttt gcttgtggga aataacttga
     2941 cagatcataa aatggcgtat cgtggtactc aaggaacttc aggactttac ctatttggct
     3001 ggacgatcgg ttcgacaggt gtgaccaaag aaagcgcaac tttgaacgat ctagaagtcc
     3061 aagcgactgt atttgaagat aattatcgtc cagagttcat gccaactact gaaaaagtaa
     3121 tgatggagct agtctatgaa aaaggaacga atcgtatcgt aggtgctcag ttcatgtcca
     3181 aatacgacat tacacaatca gcaaatacca tgtctttagc tgttcaaaat aaaatgactg
     3241 tagaagatct cgcactttca gatttcttct tccaaccaca ttttgaccgc ccatggaatt
     3301 acttgaacct acttgcacaa gcagcgctag gcgaaatcga aactgcacaa taaagaaaaa
     3361 gggttgtgac acaattttgt cacgaccctc tgtctttttt tgtgaaaagt acaattagtt
     3421 gtctttcttc gaattttttg tcacaataag cagcaaagat aaagctgtta cataaaagaa
     3481 caagcagctt tatttgattt tgaaaagaaa ggtgagaaag aagatgaata aagaatattt
     3541 tgtccaacaa gatgcatttt tagaagatta taaaagtgct tcgaagaaat taacgatcaa
     3601 tattgatgag ttggcagatc aagtactctc tcagttgaat gaaacgcaag aggaatatta
     3661 cacgatgcca gcaaatgacg ccaaagataa tgtttctcat cgttttcctt tcagtagacg
     3721 catctactca gatgaaaatg acggcacatt gaatacagag tacacatatg aaggtcagcc
     3781 atatgtgcta gaaactgatg atgatcttta aagacagttt ttgaaaaggc cgctcatcct
     3841 taagtgatga caaaaaatca tcacaattac cgatgactgt ctctttacta tcttttaatc
     3901 agaaaaaggg gttatgacaa gctttgtcaa cccctttttt tcttggtttc agattgggtt
     3961 ttcctacaaa atatttttat ctttgttgaa taggaaacaa aaaaacaata taatgaaaga
     4021 aaaagaaggt gtagaaatgg ataaaagaca actgctaata gaagaatatc tggatttagc
     4081 taatgagatt attcatcgta ataaaccaga aatggaaaaa gaatttgcag gatttactct
     4141 aaatgaacta gaagtgattg aacatatcgg gaaaatccct gatcctaacg tgacaaaatt
     4201 agcgaatgct agttacatga ctagaggagc catcagtaag ctaacaaaaa aactgatagc
     4261 tagagggatc ataaacactt atcaaagcga agagaataaa aaagaaatct attttcaatt
     4321 gacgccaatc ggacaaaaaa taaggaatag acacgagcaa ctccatcaaa aatgggtaca
     4381 gcgagatgct actgtatttg aaaatatgtc aaaagaagaa tttgacacag tatttcgttt
     4441 tatcggtcga tacagagaat ttatacgaag tagcaaatga agctactatt ttttattgta
     4501 gtgataatag gcaaaaatca taagcaaaaa tccgaatacc gtcggaagaa atggtgctgc
     4561 agcataaagc tgtcctgcga ttagcgggcc gatgacgcgc gatagagatt gtatactttg
     4621 ggtaccgccc attacaagac cttgctggtt atctgagaca gaattggata gttggccatt
     4681 gaatacagga gtaaaaatag aatcaccgaa accataacag ataatcccaa ataaaaacaa
     4741 aatcggcagt agaagaattc cagataatgt gatgaataga taggcgagga tttcactgaa
     4801 catccctatt cttgtgatct gctgttctga aaattttttg agcaatcgag gcataatgaa
     4861 caattgcgag acagtatcca agattccaat caaagagaag cttaaaccaa ccaacccagc
     4921 cttccactga aaggcgtcaa tcgaaaattg agaaaagata gattgaagag agccagctgc
     4981 caaccaaaca gcaaaacctg ctgtcaataa aggaataacg gatgaaatct tgaataattg
     5041 ttgtaattgg tgaaaaggtc ttacatggga gaaacttaaa tctactgaac gtttttttat
     5101 tggaaggctt tctagcataa atgtgtagcc ataaacggca tttaagaaag taaagagtgc
     5161 tccaataaat ataggaacag aattccccaa ctcagctaat agacccccta taataggacc
     5221 cagcgttgtt cctataccga ctaaagcacc catccatcca aaaactttgg ttctttcatt
     5281 aggttccgtg atgtcggcaa agtaagcata taaggtggag atttctcctg ccgtcaatcc
     5341 ttcaatgata cgaccaagga acaacatcca tatcgaattt cctaaaccaa aaagcaaata
     5401 tccgatactt gatcctaata aactgataat aaggatcgga tgacgcccaa aatgatcact
     5461 taagctaccg agaatggggg cagataagaa ggcagctaga gcataaacag acatgagcaa
     5521 agtgatatag aaagcttgct gatgtatgga gtgagaataa ggcagcgtta aaaacggaag
     5581 tacaggactg acgatagtaa atcctaatcc agtgaataag acagagacgt atccaaaaac
     5641 tttagaatgt ttatttgtga aactgtacat gtaaaaactc ctttatgcaa taatgttaac
     5701 ttggaaacaa aaatagtgta tagtaatttt gtttccttgt caacattcta aggaacagcg
     5761 aaatctactt aatttattta ttccgaaaag tgaagatacg catcaaaaac atgaacaaac
     5821 aaaaatcaag tagacgacat tgtaaatctg accgatcagt gaagtaaata gaaaagaaac
     5881 ctgttcttgc ttcccatgtt ttttttacgt atactgtgat tgagcggagg agaaatcatg
     5941 ttgaatttag aaaattactt aagcgctgga caaacagtca tatctaatct tttacttaaa
     6001 aattatcata ggatcggtct acaatctgaa gaatttttat ttgttctaca gttgcatatg
     6061 gcacagcttg aaggagatac tttccctgat ttgcagatga ttgctatgga tatgggaatc
     6121 aaacaagatc agatttttca aatactggat cgtctcgtaa caaatggatt tatcaagata
     6181 gaaacgacga ttgtcaacgg taaaaaagca gatcgatata atctttaccc catctatgat
     6241 ctattaggtg agtatttaaa aacacaagaa aaaaaatacg aagaaaaaac acaagaaaaa
     6301 gaaatccagt cagtgtatca attatttgaa caagaatttg gacgcccgct ttcttcgatc
     6361 gagttccagc ggatcggaca atggctagaa gaagaccatt atcagccgga aattttgaag
     6421 ctagcactgc gggaagccgt attaaatcag gcttatagtt ttaattacgt tgaccgcatt
     6481 ttgctatcgt gggaaagaaa aaatctacgt acaaaacaac aagtggaaga agaccaaaaa
     6541 cgaagaaaac agcagcttct tcaaagagaa gtagaacagt cagctcaaca agaagaattg
     6601 ccgaaagtct cactgaaaaa ttggttggag gagtagcgga atatgttaag caaacaaaaa
     6661 acaatggaag ctctagaaac gatgtatgga atgtttcccg aagcacatgg agaactcaag
     6721 cacaataatc catttgagtt attgattgca gtgatcttaa gtgcccaggc gacagatgta
     6781 tcagtgaata aagcaacgcc agatttgttt gcctcttttc cgacacccga tgcgttagca
     6841 gaagcttcaa tcgatgaaat cattcttaaa ataaaaacga ttggtttgta tcgaaataaa
     6901 gcaaaaaaca tcaaagcgtg tgcacagcag ctgattgaac gtttcgatgg gcaagtgcca
     6961 acgtcaagag aagaattgat gtctcttcct ggtgtaggca gaaagacagc aaatgtcgta
     7021 ttgggggatg cattcgggat accagcaatc gcagtagata cccacgtgga acgtgtctca
     7081 aaacgcctga ggatctgcaa actagatgct acggtcatgg aagtagaaga aacactgatg
     7141 cggaaagtac cacaagaatt atgggtaaag acacatcata cactgatttt ttttggtaga
     7201 tatcattgca cggcaagaaa tcccaagtgc gaggtatgtc ctttgctatc aatatgtcaa
     7261 gatgggaaaa atcggatgcg tctaaaagaa aaggcgctaa aaaagaaacc acgcctgtag
     7321 acgtggtttc tttaattgca aataaatgta tgaattatct tgttattatg gagaaaagct
     7381 gtttcgttta tcttttttgc atgaaaaagt aaatgtacat actcagtagc taggatggta
     7441 acttattaca atttattgcg ataaaaataa aagttgttat ttttattggg aaaatacccg
     7501 atgtagaaaa taatgccttc caatatacct ataaaataag tccagtattc caaaggatta
     7561 tataaataag tgacgtatct atctaaaggt gtaataaaaa tgataagtga taataaaaaa
     7621 ataccattta taactaaaca taaactaatg gatttgggcg gtttcaggct catcagaaaa
     7681 atatagattc tggtgaacaa caaccataaa aaagtgagaa tcattgcatg agagcttctt
     7741 tccataaatt ttcctttaag agtagtgctt aaaagtacag aaaaaataat aaatgcaagg
     7801 ataggagcaa ttaaagtaaa tttatttaat aataaacaaa atttttcagc ttttttattc
     7861 ttggaataac gaggtattgt tttcgataaa aaaagatggt gcagacaaat tatgataatt
     7921 aagcatacta tcagataact caaaatgaag ccttctttca aaaaattatt caaaaccata
     7981 tattgtattt tttatttgat aataacaatg ggattattaa gaagtggcac taaataaaag
     8041 actagtgtgt atttagccga tttaattata aacaagtaaa tggataaagt atatgtaatg
     8101 ataaaagaaa aatggatttt ctaattttta agctaaatgt tagatcacac atgtatatgc
     8161 tggatagtct tataaaaaat catgtaatac agcttggtac atgattttta tttgtagctg
     8221 ttttttacat agacacaaaa aagaaaccac gcttgtagac gtggtttctt ttttactatc
     8281 tgtttccaga agctgaggtg ctgctggatg gtgtatggtc atttgcagca tccgctccgc
     8341 tagttgctgg ctcactggaa gaagcaggag gagcagatga agacggtgtc gagctttctt
     8401 cggatgaaga agaaggaacc gttgttgaag acgctggctc gcttgtttct gctgtcgtgc
     8461 tttcttttga ggactcagca gttgattcgc tttgactagt agagctagag ctttgagtcg
     8521 ttgtctctgt tgttgagctt ccaggagtct ggacatagct ggaagatgga atagttgttg
     8581 atggtgtaat tgcattcgat ctagctgtat attgatcttt gtagtacagt tcaccgccca
     8641 cacgaattaa tccgcttggc atctcccagt ctgtatttgt cacgttagct gaaacatact
     8701 gcataagctc gcgataaaca tcagaggcta cgtgtgacga ttctgaagtg acaggtgtca
     8761 tttttttatt gtaacctgtc cacacggaaa tcgaataatt tggcgtatag cccgcaaata
     8821 ggatatctgg atacacccca gaagaaatac caagttttgc atactcgtca tctgtgtaat
     8881 tggatgttcc ggtcttaccg gcttgatata acccagcgat ttgcgcattc gtaccagttc
     8941 cttcagtgat cgtatctttt aaaatgtctg tgatcatata agctgtttct ggagacatgg
     9001 ctgtttttcc atctggctca taagtttctt ctgttccgtc ttggaagacg attttattaa
     9061 catattgagg tttgtaataa gtccctccat tggcgaaagc cgcataggct gctgccattt
     9121 ttaaggaaga agctccgtat tttgtcccat cttgttcttc cgtattacta gagatcgcat
     9181 tcgattgatg gatcgtactg tattcgatcc caagattttt taagaaagaa gccactttat
     9241 ctgaaccgac ttcattgaat aatttgactg ctggaacatt acgggataag taaagtgctt
     9301 ggcgtaaagt aatggtgccc atatattgat tatcccagtt tcctacagga gtcgaagtcc
     9361 cttcatagtt atacggtgcg tcagtaatag tttttccagt agaatatttc aaatattcaa
     9421 aagctggtcc gtaatctgtg acaggtttca tcgtagaacc aaaatcacgg gaagtattca
     9481 ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg acgtccgcca atctgggctt
     9541 ttactttccc ggtattggtg tcgataaggg tggaggcaac ttgcatctcg tcgtctgggt
     9601 aagagacata ctgatccgtg ttgacgatat catatagttt cttttgggcg tccagatcca
     9661 aattcgtata aatctctaaa ccgtctgtgt agacattctt atctgttttt tcttgtactt
     9721 cattgatgac ttctttgaca taattatcga cgatcttagt attgtcatcg gattgagtta
     9781 gttcttgcag accatccgtt accggtacat taacagcttg atcatattct gtttgagaga
     9841 ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg tcttttcttc gcctgatctg
     9901 gataaacata aggatcatag gcggaagggg cttgaggcat cccagcaagc aatgctgttt
     9961 gcggtaaaga aagttctgat aatttcttac caaagtacat ctcagaagct gtctccatcc
    10021 cgtacaagcc gtttgacata tacactttat ttacgtagta ggtcaagatc tcttgtttcg
    10081 attttttttg ttccaatctt acagccatcc acgcttcttg ggccttgcgt ttcagagtct
    10141 gatcctcggc actggtggag aagaaggaaa gtttgatcaa ttgctgtgtc aaagtacttc
    10201 cgccttgcag tccgccagag gtaaagttag agagggcaga accgatgatc cgaatagggt
    10261 caaccccgat gtgtttataa aaacggcgat cttctaccga cacgatcgca tcttctaatg
    10321 ttttcggtaa ttcattcgca gaaatttttt cacgcttttc tgcgcctaaa tcttcgaata
    10381 gttcgccatc ttgtgtatat agtttggaag atacggtagc gtctaatttt ttatccgtca
    10441 attcgggcgc atcttttgcg taataccaga aaagtcctac acctgctaaa aagaggatac
    10501 agaaaaatga gagtattcct aaaaggatct tgataaataa tcctttttta tgtgttccag
    10561 aggactttcc agaacctttg ccagaattct tcttcacact ctttttagga gtaggctgtt
    10621 tctgtcgtct agagcttcta gtttgttcat ttgccattct tgctttctcc ttcaatagat
    10681 aatgttgata aatagaagtc aaccgcgtcc aaataaggaa tccgcggaga aataccagtt
    10741 ttgatctcta ttcctagttc ttcgatcaaa gataaaggta gtgatttgcg tcctttttcc
    10801 ttttgggcgt cccagtaagc aatcaaatga acactattca aataaaaaca gcgcttcaaa
    10861 gacgaaaacc ataacaagac aaagcagacc ccattttgtt cgaggcatgc tttcatatgt
    10921 tcgatttgat gggcatggaa gtttttaaag ggaaaagatg ttttgttctt ggtttctttt
    10981 gcttcaaagt ccaagtaaaa gcctcgatag acgccattat agtctgtagt tgaggcttgg
    11041 gtaaagtagg cttccttgat cacagcagca cttctcttag gatagtctac tttcacgatt
    11101 tggatagggg taggcttttt atggataacc gccaacccgc gggaaagata atatttatta
    11161 ctttcattga ttgcttcttc aaagcgcatc ccccgattgc cgaattcagt gatctgattt
    11221 cttttttctt gaattttttc gacgggagag gcattcttat aataaggttg gccatttgga
    11281 tagcgcaata ccatctttaa tcacactcct aggccacatt atagcaaaaa agcggataga
    11341 aagaaaagga atctgagatg gataagtcaa aagtaatcta tgtgtctggg tatcgaagtt
    11401 tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca agttgttaaa aatgttttaa
    11461 aaaaagaact ccatcagtta gcagaagaag ggctggaatg ggtacttgtc agtgggaatt
    11521 tgggcgtcga actatgggct gtgcaaatag tagcggaatt gaaagaagaa tatccggaac
    11581 tatctcttgg tattttgtat cctttttctg attttggtaa taactggaat gaagggaacc
    11641 aagaaaaaaa acaattagca gaacaattag cagattatgt cgaagctgtt agccatcaac
    11701 cctaccaatc acctagtcag ctaaagaatc acactcgttt tatacttgag catacagtgg
    11761 gctgtctgct gatctatgat gaagaattcc ctggaaaaac gaagtatttt tttgaagatg
    11821 cacagaatta ccaagcagat catccctacg aaattcggct gatcagtatg gacgatcttc
    11881 aaaattttag tgaataatcg atattgtgtt tgatttatca acttttttcc tatacaatga
    11941 aaaaggtaga aatgttatta cgtaaatgaa aactagaggt gtaaatatgg cgaatttggt
    12001 ttatagtcct aaagacattt tacaacaaga attcaaaaca aaaatgcgcg gctatgatcc
    12061 cgttgaagtt gatgaattct tagacaatat catcaaagat tatgaagctt acagcaaaga
    12121 actccttgct ttacaagaag aaaacgatcg attgagtgca aaagtagctc aattatctaa
    12181 aactcaagga gcagctcaaa cacgcgtgca acaaacagag gcaccaaaga gcgcagcagt
    12241 gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa cgtgaagtat ttggtaaaaa
    12301 gcttgatcaa caagcaagtg cagtcaaacc agcacaacct aatccaaata actacacaaa
    12361 tgcagacaca agcttagatg ataatgaaaa aacacgtcaa ttttaatttt tgattaatga
    12421 atttgcggtt ttcgggtaat cgcggtctga cttgatcagg ctgaggaaag tccatgctcg
    12481 cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac cataagctaa ggtactcttt
    12541 ggagtaacgg caggaaaaca agctaaggct tcggctatgc ttaagtatcc ttgaaagtgc
    12601 cacagtgacg aagcggctgg ggaaacccat ccggtggaac gcggtaaacc cctcgagcga
    12661 gcaacccaaa caatggtagg ggcgcttttt gtctggaaga agaacaggac aaaaaggcag
    12721 tataactgca gatagatggt taccgctttg ttttttctcc ctggaaaagc aaagtacaga
    12781 acatggctta tagaaaaccg caaacatcag ggaattaggt cgtgacaaaa gtaatgcgtt
    12841 ataagaaaca agaaaaacat ttgaaagcta tttttcaata cttcagatga atcgttctta
    12901 ttacaaaaca aacttttgta tcaccggtta ttcggaattt gagtaaaagt gtggggtagg
    12961 actgatgtcc cacctccttt ttttataaat aaggagattg tgtatggaaa aaaacaggaa
    13021 gttcaacctc gtcgcaactg cggcaagcgg gctagaagca ctggttggta aagaattaag
    13081 agatcttgga atcgaatgcc aagttgagaa cggacgtgca cgttttgaag gaacaatgga
    13141 aacgatcgct acagctaatt tatggttacg tacagcagat cgagtaaaaa tcgtggttgg
    13201 ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac gtaaaagcat tgccttggga
    13261 agattttctt ccactagatg cagcttttcc tgtagctgga aaatcgatca agtcgaaact
    13321 atatagcaca cctgattgcc aagcgatcac aaaaaaagca atcgttgaac gtctccgtac
    13381 ctattatcac cgtccagctt ctgtaccact gacggaaact ggcgcacatt ttcagttgga
    13441 agtagctttg ctgaaagacc atgtgatggt aactctggat acgacaggac caagtttatt
    13501 caaacgtggt tatcgcttag aaaaaggtgg agcaccattg aaagaaaaca tggcagcagc
    13561 acttattatg ttgacaaatt ggcgaaaaga ccgtccattt tatgatcctg tatgtggttc
    13621 aggtacgctt tgtattgaag cagccttaat aggccataat atcgcaccag gattcaatcg
    13681 tgagtttgct tgcgaatctt gggattggtt cagtcaagaa atcatggaaa atgttcgatc
    13741 agcagccgaa gaaaaagcag attatgatat tgaattagat attactggtt cagatatcaa
    13801 tggacggatg attgaaattg ccaaagccaa tgcagaagaa atcggattag gctcttcgat
    13861 tacctttaag caacaagcag tcaaagactt caaaacggat aaagaatacg gggtcatcgt
    13921 ggctaatccg ccttacggag aacgtctagg agaagaagaa actgttcgga aattatataa
    13981 agaaatgggc gatgtattcc gtccattaaa aacatggagt aaatatatct tgactagcga
    14041 cttagcgttt gaggaatttt acggacaaaa agcaaccaaa aaaagaaaat tgtacaatgg
    14101 tgcattgcgt acggatctat tccagtattg gggaactcgt ccgcctagaa aaaaagagaa
    14161 agacgaataa tcttactaat tgattcattc aattggatgt ttggaggaat agggaatgaa
    14221 agaaaaagag tttttacaag aagtaaaaga aatcaatctt ctacaacaag ctttaggaat
    14281 cctcgattgg gatacacaaa caggtatgcc tgaaaaagct agtgcatacc gaagtgaagt
    14341 agacagctat ctttacagca tttatttttc aaaaaaaatc ggtccaaaaa tcaaagaagc
    14401 cattcgctat ttttctgaac atccggaaga attgtcggaa gttggaactg ccgtttttgc
    14461 caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg gaagatctta tgagaaaata
    14521 tacagctgcc gtttcttcag cacacggaca atggcaaaaa gctagagaaa cacaacaatt
    14581 ttctgatttt caacacgcat tgaatcaaaa tattgaatta acaaaacagt tgatacctta
    14641 ctggagaaaa gatgaaaaga cgaattatga tgttttgctg aatcaatacg aacccgggat
    14701 gactgtcgaa atccttgata aggtatttga tcagcttaaa gaaggaattc tttcgatccg
    14761 ccgtacgctc caagaaaaag ggactgaacc tgaaacagac tttttaaatc ggagaatgac
    14821 gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa caacttggtt atgattttag
    14881 tcgtggtcgt ttagatgaca ctgtccaccc atttatgact ggaattaatc cgaatgatgc
    14941 acgaatcacg acacgatgga acgaaacaga tttcaaaatg gctgtttttg gagtgatcca
    15001 cgaggctggt catggtatgt atgaacaaga catcgatgat aaatatgcgt atacaccggt
    15061 ttatgaaggt acatcaatgg gtatccatga atcacaatcg ttgtttaacg agatcattat
    15121 tggaagtagt cgcagcttct ggaaaaagca atatcctttc tttcaagaat gtgcagaagg
    15181 aacattcgat gatattcctt ttgacaaatt ttatcgttca ttgaaccata caaaatcaag
    15241 tttggttcgt atcgaggcag acagtttgac ttaccctctc catatcatca ttcgttatga
    15301 aatcgaaaaa atgattttca atgaaggggt agatgttgct gaactacctg aaatatggaa
    15361 tcaaaaatat gaagagtatc tgggaatacg accaacaaat gatgtggaag gcatcctaca
    15421 agacgttcat tggtcaggtg caagctttgg ttattttccc tcttatgcat tagggtacat
    15481 gtatgcggca caattacttc attctttgca gcaagaaata gatgtggaaa atgttctagc
    15541 gtcagacgat tattctccaa tcaaaaactg gatgtcagag catatccatc aatatggtgc
    15601 atctagaaaa ccaaatcaat taatcatgga tgcaacacat gaaccgctta atccgcaata
    15661 cctgatcgac tatatgaaag acctgtattt taatgtatat caggtaaaat aaaatacaat
    15721 ttacaaagat agctgaaatt catctaatct atagatgttt tcagctattt acttaagaaa
    15781 gaagtggaac gaatggataa attacaagtg atcgctgatt atagtatgga ccaattagcg
    15841 catgatcaaa caggacatgg gagcgatcat accaaaagag ttgtcaagtt agctgaaaaa
    15901 attttggaga cggaaccgca ggcagaccgc tttgtgacgc ttgcagcagc ttacctacat
    15961 gatacgattg atgataaagt cgttaaaaat gagaacgagg caaagcaaca gcttcgtgta
    16021 ttccttcgct ctttgccaat agtagaggaa cagatctcga tgattttttc cattatcgaa
    16081 aatatgtcct tttctaaaaa tctctctgaa gcggccgaac tatctctgga aggtaagatc
    16141 gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg gcattttgcg tactgcttat
    16201 ttcggcggtg gacatgggca cccgattttt gatcctgaac tgtatccgca aacattcaag
    16261 gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc atttttatga aaaactgttc
    16321 ttgctgtctg ataaaatgaa tactgattac ggatatgaag aagccaaacg aagagaaacc
    16381 ttcatgcgtg aattcttaga agaatttttt ctggaatggt ctattgacga tgaaaatttt
    16441 actccagaca attggatcaa aaataacaaa aaatgatgga ggttgtgaca gaagtggtca
    16501 gctccaagat atttcggatc attttccgta aaagttactt ctggaacact gtttaccaag
    16561 aggtcgtgag cctgccgttc agctccgagt cacaaggaac aatttcaaaa aatagcttct
    16621 cgtgtttgat gaatattgta acttgtgatt cggaattggc aggagaacac cgtttattcg
    16681 gaagaatagg actgtgacaa gacttttgtc acagtccttt tttcgaattc aacaagtaaa
    16741 acttttaaga aatattcaga gagtaaaatt aaaaattgct gataaacgaa gatggaaatt
    16801 ttatgtattt ttattggtct atgcaataaa taaattaaat attcagaata caattgattt
    16861 taacgaaagg gcttctatca atggttgtgt tgtatttatc tgcccaggta gagtaaaata
    16921 gatcattatc taaaaggtaa ttgttcatac tatataagtc atttgaaagt aaaaagatac
    16981 tgtaaataaa aaatgtggtt aaataattct cataatcagt gtaagcgtta tcaaaaatat
    17041 agcatacttg ctttgtagat gaatgacacg atatatgttt ggtatgttct gcaagacaga
    17101 tgaatatgga ggtggagaca atgtcttcca aagaactaac aatgtattta gagaaagaat
    17161 tggatcaact gaaagaaaag gggttataca atacgatcga catactagaa agtgaaaatg
    17221 gtgcttgtat catcgtagac ggaaaaaaga tgatcaatct agcatccaac aattatcttg
    17281 gatttgccaa tcgcgaagaa ttgaaaaaag catgtattga agcaaccgaa acttatggtg
    17341 taggagcagg agcagtccga acgatcaatg gttcgctgaa aatccatcag caattagaag
    17401 aaaaaatcgc agaattcaaa gggacagaag cagcaatcgc ctttcaatct ggattcaact
    17461 gcaatatggg cgctatctct gcagtaatga caaaagaaga tgctatctta tcggatgaat
    17521 taaaccatgc atctattatt gacggttgcc gcttatccgg agcaaaaatc atccgaatta
    17581 aacatcaaga tatgaaagac cttgagaaaa aagcaaagga agcgatagaa agtaagaaat
    17641 ataaaaaaat catgtatatc acagatggtg ttttctccat ggatggagac attgcacgat
    17701 tgccggaaat tattccgatt gttgaaaaat atggactgat cacctacgtc gatgatgcgc
    17761 acggctcagg cgtaactgga aaaggtgcag gaaccgtcaa gcattttgga ttaagcgaca
    17821 aaatcgatat gcaaatgggc acgttatcga aagctatagg tgttgttggg ggttatgtag
    17881 ctggatcgaa aacactaatc gactggttga aagctagaag tcggccattc ctattttcta
    17941 cttcgctaac tccaggtgca gctgcagctg cattggcttc gattactttg atgcaagaac
    18001 atccggaact tgtcgaaaaa gtttgggaaa atgctaatta ttttaaagaa gaattgaaaa
    18061 aagtaggcta taacatcggt atgtctgaaa cacctattac tccagtcatt ttgggagacg
    18121 aaaaagtgac tcagactttc tctaagaagc tgatcgaaca tgggatttat gctaaaccaa
    18181 tcgtttatcc aactgtgcct cttgggacag gcaggatcag aaatatgccg actgcggaac
    18241 atacgaggga aatgcttgat gaagcagtag cagtttatca aaaaatagga aaagaaatgg
    18301 aaattattta actaaattta gttttttcgt ctatttaaaa ttagagaaca tgttcacgca
    18361 tatattgtta atcatctgaa gatagtagta aaaaagatca gggaggatca tcatgaaaaa
    18421 agtattggtg acaggatgtt taggacaaat tggttcagag ttgatcacac ggctacgtca
    18481 agaactaggc agtgaaaatg tcattgcgac agatatacgc atgcctgaag tcattgagaa
    18541 aaacgatatc tttgagatcc tagatgtaat ggattacgaa aaaatgcgca gcatagcaga
    18601 acaatatcaa gtggacacat tgattcattt ggctgctctt ttgtcagctg ttgcagaagc
    18661 aaaaccacaa ttagcctgga atttgaacgt gactgggctg gtcaatgcat tggaagtcgc
    18721 tagagaattc cagctgaaat tttttacgcc aagttcaatc ggcgcgtttg gtccaaatac
    18781 tccaaaagat cataccccac aagatactct tcagcgtcct acaaccatgt atggggtaac
    18841 gaaggtagct ggagaactac tatgtgatta ttattatgtg aaatatggcg tagatactcg
    18901 tggtgtccgt ttccctggat tgatttctta tgcaaccttg ccaggaggcg gtacgacaga
    18961 ctacgcagtc gatatttatt attcagcaat caaagaagga aagtacacta gtttcattga
    19021 tcaaggtaca tcaatggata tgatgtatat gccagatgcg atcgatgcca ttataaaact
    19081 catgaatgca gatcctgatc gattggttca tcgaaatgcc ttcaatatat cggcgatgtc
    19141 ttttgacccg gaagagatca aagcatctat tcaaaaaatc ataccagatt ttgaaatgga
    19201 ttatgatgtg gatcctgtac gtcagtcaat agcagaatct tggccaaatt cactagatgt
    19261 ttcttgtgcg caagaggaat ggggcttttc gccgaaatac gacttagatg tgatgacaaa
    19321 ggatatgttg gaaaaactga aagaaaaatt ggtagagacg gtataaaaca agaaccacat
    19381 ccaaaaagtt ttttatactt ttgggattgg ctatggtatg aaaggggaca agggatggaa
    19441 gaatatcaag ccacgccagt aaaagaagta aaaatagaaa atgaattaaa acgtacgatg
    19501 ggatttttta ctgctttatc gactgttatg ggcacagtga tcggtgcagg ggtatttttt
    19561 aaagccgcaa gcgtagcaga agttacagga tccgccagtt tgcatatgtt ttcttggttt
    19621 ctcggtggta tgatttctgt ctgtgcagga ctaacaggag ctgaactggc agctgctatt
    19681 ccagaaacag gagggatgat taaatatatc gaacggattt acggaaatac agcagctttt
    19741 ttattgggct gggcgcaagt cgtgatttat tttccagcaa atgtagcagc cttgtcgatc
    19801 attttcggta cacagttcgt caatctattt ggtctttccc agtcaatgat tgttcccgta
    19861 gctgttacag ctgctgtatc gatcctgttg atcaattttt taggttctaa agctggaggg
    19921 gcttttcagt cgattacgct tgtctgcaag cttattcctt tgtttgtgat cgtcattttc
    19981 ggtttgttcc gacaagaagg agtagacttc caactgtttc cgatccaagc aggagaaaat
    20041 ctttcatttt tctcagcctt gggagcgggc ttgctagcaa cgatgttcgc atacgacggt
    20101 tggatacacg taggaaatat ttcgggagaa ttgaaaaagc cggcgaaaga tttgccaaaa
    20161 gcaatttctt taggaatcat cggaatcatg atcgtttatt tattagtaaa tgcggtgttc
    20221 ttaagaaccg catctatcga cggagtcgca ggaaacagca atgcggcaag tgacgttgca
    20281 aaaatgattt ttggcggctt cggtggaaga ctggtcacag ttggtatctt gatttctgtt
    20341 tatggaacca tcaatggcta tacccttact ggaatgcgtc tcccttatgt tatggcaaaa
    20401 gaaaacaacc tgccttttag caagctcttt gctaaacttc atgataaaac taaagttcct
    20461 gtagccgcag gtatcttaga gctagtcatc gctattggca tgatgatgat cggtggtttt
    20521 gatacactaa cggatatgct gatctttgtc atttggattt tttatacgat ggtctttgtt
    20581 ggtgtcattc ttcttcgtaa aaaagaacct gatttgtttc gaccatacaa agttccaatg
    20641 tatccgttta ttccactagt agcaatcatt ggagggactt ttatcgtatc aagcacgctt
    20701 atcactcaga cctttttagc ttcgatgggg atagtattga cacttgctgg tatcccgatt
    20761 tatctctatt taaaacgtag atagctaaaa cattcgaatt ttattagaac tgcacttctc
    20821 tgccaaacaa aatattttat ttaaatgagg agtacgtgga agaaatgtca aaagtactcg
    20881 ttaattttga tcacgtaaat gtcgttttgt ctacgatgaa atagcaaagt aaaagacaga
    20941 aatcgggctc aaccgatttc tgtcttttac tttgatttgc gtaactgaac tattcggcgt
    21001 acgccgcgat aaatatttaa taaaaccaag caaccagaaa tgaaaatggg aagccaaatc
    21061 agatagacag ggtagccaag cactttagac aaagcatgtg gagtcatttt atccatttca
    21121 agaagtgctt gctgccaagt ctgaagattg aagactagca tgaaacccga aataatcagg
    21181 agaataaatc ctaaataatg aattgtagtt ctttttctta cataagcgat cgtagcataa
    21241 aggaacagta aaaaaatagg tacgccaaaa acaaagtaca tattcagcat ctcgacaccc
    21301 cctttgtgtt tatcttactt tactttgaaa gaaatgagaa ataatagcct cgttacttgc
    21361 ttgaaacagc aaaggactgc taagataaga gagatacaaa gaatcaagga ggagtcatat
    21421 ggcaagttat atcgaaccga ttcgttttgc gctgattgtt tttccatttt tagccttagc
    21481 gatctcatct gtttttttta tttatgaata tcgaaagtat ggaacatttc tagtaacaag
    21541 ggcagttatt ttgtattctt tcgtttttta tttattgtgt gcgtattttt tggtgatctt
    21601 accacttccg gcaaaggaga cggttgccca aatgacaggg ccaaagatgg agctgcattt
    21661 atttgcttca tggcaacatt tcagagacaa cacggtattg gtcttgtctg aacctagtac
    21721 ctacctgccg gcaatgaagc agagtgtatt tttggaacca gcttttaatg ttttattact
    21781 cataccattt ggcatttatt taagatatta tttccggctt tcttttatga aaacgatgat
    21841 tgctagtttc tgcctttctc tattttttga attgacgcag ctaagtggac tttattttat
    21901 ctacccaaga ccataccgct tatttgatgt gaatgattta gtgacaaata cattgggtgg
    21961 aatcattggt tttgtcgtta cgccgttatt tacatttatg cttcctacaa gaagacgaat
    22021 ggatgaactt tcttatgaaa aaggaaagac tgtctcattg actcgacgct tggtcgctta
    22081 tttgatcgac tggacatttt tatctcttat taatttggta gcggcgattt tctttaaaat
    22141 tgcgttcgat tcaggggcag ttacttctca tatctggtgg cttttattag aagtaggggt
    22201 atactttatg attgtaccgt ttataaccaa tggatggaca cttggcaaaa aaattgtgcg
    22261 tatccgtttg gtggaagaag accgcgaacg aatttcttta aaagcattga tgatccgcta
    22321 cgggtatctg tactttcttt tttatggttt aagctggttg gggcagttgc ttcagcaaat
    22381 catgaattcg acaacgaata cggtgttgca gatgagtgca gtattcattt ctctcggtgt
    22441 cttgttacta caaggtattt ttattttaaa tatcttgcta tcatttttga gaaaaaaacg
    22501 gattctgttt tatgaaaaag ctagtcacac atatactgtt agcacgattg gccaaacaaa
    22561 ggatgatgat tctcgaaatt agctgagcaa aaacgctttg tctgtgttat aatgccaaag
    22621 tcgtgataat ttaaagagaa gaggtgcacg aatgaataat tttgatatat tccgcttcaa
    22681 accgtttatc aatgaagcgt taaccgcaaa gggcttcaaa cagcccacag aggtccagga
    22741 acgtttgatt cctttaattg caaaaggaaa aaatatcatt gggcaatctc aaactggtac
    22801 aggaaaaaca catacgtttt tgttgccttt gtttgaaaaa atcaatgcag aaaaagagga
    22861 agtacaagtt gttattacag ctccaagtag ggaactagct gcacagatct atgaagcagc
    22921 tacacaaatc gctaaatttt ctacaccgga gatccgtgtc tcgaattttg tcggcggtac
    22981 agacaaacag cgtcaattaa atcgtctgca tcaccaacag ccacaggttg tagtaggcac
    23041 gcctggtaga atattagaca tgatgaatga acaagcgctg aagattcaca ccgcatttgc
    23101 atttgttgtc gatgaagcgg atatgacatt ggatatgggt tttttaaatg aagtagacca
    23161 aattgcaagt cgattaccag aaaagctgca gtttttagtt ttttctgcca ctattccaga
    23221 aaaacttcgt ccattcttac gaaaatacat ggaaaatcca atcattgaag aaatcaaacc
    23281 aaaagcagtt atttctgaag acattgaaaa ttggctgatt tcaacaaaag gtaaagatga
    23341 gaatcaaatg atttatcaac tgctgacaat tggtcatcct tatttagcaa tcgtatttgc
    23401 caatacaaaa cagcatgtgg atgaaattgc cgattacttg aaaaaccaag gcttgaaagt
    23461 agcaaagatt catggagaca tcaccccaag agagagaaaa cgagtgatgc gacaagtaca
    23521 gaatctggag tatcaatatg ttgtagcgac tgatttagct gctagaggga tcgatatcga
    23581 aggtgtctct catgttatta atgcagaaat tccagaagat ctagacttct ttatccatcg
    23641 tgtcggacgt actggacgta atggcttaaa aggaacggcc attacgttat attcgccaaa
    23701 tgatgagcaa gccattgatg cgatagaaaa attgggcgtc tcatttgaac caaaagaaat
    23761 caaaaacggg gaaatcgtca aaacatacga tcgtaataga cgtgccaaac gagaaaaatc
    23821 aaaagatgta ttagatccca cgctgattgg tcttgtgaag aagaaaaaga aaaagatcaa
    23881 gcctggttat aagaaaaaaa tcgattgggc aattgcagaa aaaaataaaa aagaacgcaa
    23941 aatcgaacgc agacagcaaa gccgtactgc tcgtaaaaat aagaaaaatt cacataaata
    24001 aaaaacaaga ggttgtgaca aaaggcgtgt cacaatctct tgtttttaag ctagactttt
    24061 tttcattccg atacaaagta cttcatcttc aataaaaggt tctgtgcaag tatgataccc
    24121 ttgtttttca taaaaatcta cagccgtcat ttcagctact acataactag tctgacaacc
    24181 ttctgaaacc gcccgttttt cagcagcttc aagcaactga ccgcctaacc cttttcctcg
    24241 gtattctttt gctacgcaaa atcgttccgg cttgaagcaa gtaggtgttt tcttttcata
    24301 acgcaaagta gctactggtc ttttttgatc aagaaataga taataggttt gttgcgggat
    24361 atcttttccg tcgaattcta gttctgcagg aatcccttgt tctaatacaa aaacttgata
    24421 tctcaaaaag cagctagctg cgcgtatcca ttcatccgct ccaactattt ttttcatcat
    24481 attcctcctt gtcatgaata gcagagatgt attgattcct ttattcttat cgtaccaaaa
    24541 atcgtggagt atgaccaaaa ggaaatgaaa ataaaaattg agatattgac agtgatcaat
    24601 agaatgcgta tactaattaa gtacgaaaag gatatgattt gttttttaag actcacagaa
    24661 aattctttct tggctgaaaa aagaatagtc actaatcaaa ttgctccctt tttctatttt
    24721 ggtagaaata ccgacgcagc tcagcgttat cgagtttaag aggtaatgat tacacatcat
    24781 tgcaatcaag gtggtaccgc gagttttcgt ccttggctgt gatggtgttt ttttgtattt
    24841 aatttataga aagcaggtaa tgacatgaaa caattaagta gtgcacaagt tcgacaaatg
    24901 tttcttgatt tcttccaatc taaaggacat acgattgaac ctagtgcgtc tttgatcccc
    24961 gtgaacgatc caaccttatt gtggatcaac tctggtgttg caacattgaa aaaatatttt
    25021 gatggttcag ttgtgccaga aaatccaaga atcacaaatg cccaaaaatc tattcgaaca
    25081 aatgatatcg aaaatgtcgg taaaacagca cggcaccata cgatgtttga gatgctgggt
    25141 aatttttcga ttggtgacta tttcaaaaat gaagccatcc actgggcatg ggaatttttg
    25201 actgatccaa aatggatggc attcgatcca gaaaaacttt atgtaacagt ttatccaaaa
    25261 gatacagaag caaaacgcat ctggcatgaa gaagttggcc tgccactaga tcacattatt
    25321 gatgtagaag acaacttctg ggatatcggt gcaggcccat gtggtccgga tacggaaatt
    25381 ttctatgacc gtggagaagc ttataacgat ttagcagaag acgatcctga aaactatcca
    25441 ggaggagaaa atgaacgtta tttagaaatc tggaacttag tattttcaga attcaaccat
    25501 aagcctgata acacatttga accacttcct cataaaaaca ttgatacggg gatgggattg
    25561 gaacgcatgg tctcaatcgt tcaagatgcg tcaacgaact ttgagacaga cttgtttatg
    25621 cctattatcc acgccgtaga aaagctaagc aaacaagtaa aatatggtga aaacccagta
    25681 acagatattt cttacaaagt aatcgcagat catattcgcg ccctttcatt tgctattggc
    25741 gacggtgcat tgccatcgaa tgaagggaga gggtatgtct tacgccgttt gcttcgtcgt
    25801 gctgtcatgc atgggaaaaa attaggtatt gaacaagcat tcttatatcg tttagtccct
    25861 gttgttgggg aaatcatggt aagttattat cctgaagtac ttcaacaaaa agaatttatt
    25921 gaaaaagtta tccgtacaga agaagaacgt ttcaatgaaa caatcaatga aggtttggaa
    25981 atcttaaatc aagtgatcca agaagtaaaa aacgaaaacg ggacgactct agcaggaaaa
    26041 gacatcttca aactatacga cacatatggc ttcccggtag aattaacgga agaggtggca
    26101 gaagatgaag gactgaaagt tgatcatgct ggttttgaaa aagaaatgga tgcacaacgc
    26161 gaacgtgctc gctctgcccg cagtactgaa aaatcaatgg gggtacaatc tgctttattg
    26221 acagatatca aagtagaaag taaattcatt ggatacacag aacaagaatc agatagtcaa
    26281 ttattggtca tcgttaaagg tgagtctctt ttgaatgaag tcgcagaagg tgaagctcaa
    26341 atgatctttg atcaaacacc tttctatgca gaaatgggtg ggcaagtagc ggatcaagga
    26401 tggatcatga atgaagtagg cgaaatcgta gcagttgtta cagatgttca aaaagcgcct
    26461 aatggtcaat ttatgcacac cgtagaagta aaagcaccaa tgagtgaagg acaaacctat
    26521 catctattga tcgatcaatc gatgcgcaat cggatcatca aaaaccatac agcaacccat
    26581 ctactgcaca aggcattgaa agaagtactt ggcgagcatg caaatcaagc tggttcttta
    26641 gtcgctcctg gacatttacg attcgacttt acccactttg gtcaaatcac ttcagatgaa
    26701 ttagctgaaa tggaaagaat cgtcaacgaa aaaatctggg aagctatccc agttgaaaca
    26761 attgaaacag atatcgatac agccaaaaac atgggtgcaa tggctttgtt tggtgaaaaa
    26821 tacggacatg acgttcgagt agtaaatatt ggcggctggt caatcgaact gtgcggaggc
    26881 acacatgtag caaatacaga aaacatcgga atcttcaaaa tcgtttctga atcaggaatt
    26941 ggtgcaggcg tgcgacgaat cgaggctgta acaagtaaag aagcgtacga actacttgaa
    27001 gaagaagaaa aacaactaaa acaagtagca acaattgtta aatcacctca attgaaagaa
    27061 gttgtttcaa aagcggaaca attgcaacag caagtaagag agctacaaaa ggaaaatgaa
    27121 caattagcta gcaagttagc gaatcaacaa gctggtgatg tattcaaaga tccagaaacg
    27181 atcaatggaa aaacaatcat tgcagcacaa gtaaaagtga aagatatgaa tcagctgcgt
    27241 caattagctg accaatggaa acaaaaaaat atttcagata ttcttgtttt agcaaacgaa
    27301 caagaaggaa aagtcagtct gctagcggca atgacgaaag aatcaaatga agctggacta
    27361 aaagctggcg acttgattaa agcaatcgca ccgaaagtag gcggtggcgg tggtggacgt
    27421 ccagacatgg cacaagctgg cgggaaaaat cctgctggta ttccagatgc acttaaagct
    27481 gcgaaagaat gggttgcgca acaatagggc gtttacgttt gacagaaaaa caaaagttca
    27541 aacattgaac gaaacatgat tatgggggcg aagacaaaca agtcttgccc tcatactttt
    27601 ttcaattgga tggcgcatct aatcaaatgg tcggattcgg atggtcttta gttgacgaaa
    27661 gtgacagaaa tttttacgat tagtataaat aaaaaggggt gaaaaaatga atagtagaaa
    27721 taagaccttt cgactagtct tacgtgcgat cttgttagca atcatcatcg tacaagcgat
    27781 ggttccttgg ctggggttta ttccattagg attcattagt ttaacaatta tccatatcac
    27841 agtaattatt gctgcagtag tattaggccc aaaagatggc atggtgattg gcttgttttg
    27901 gggaatcgct acaattgttc gtgcctatgc catgccaaca acgccatttg atacattagt
    27961 gttcacgaat ccgattatct cggtagttcc cagagtactt gttggcttag tagcaggctt
    28021 ggttttccac tggatttatc aaaaaaataa atcaataacg atcagctcag cctttgcagg
    28081 ggtactgggt tcattggtca atacagtctt agtgttagga ttcatgggat tattttatac
    28141 aggtgcaacg gcagatgcct atggtgtaga ctctgctctt ttatttaaaa ccttagcagg
    28201 ggttgcagca atcaatggga taccagaagc aataggtgcg ggaatcataa caccattact
    28261 agcaaaagcg ttatttgcag caacaccgtt gaaaccagaa taatgaccat cttttaatag
    28321 taataggacg cacgatagat agaaaaagga agagaggaga ggaaaacaat cgtcctctcc
    28381 atctttttac ctgagcaaca agcaaactag aaggggtaaa aaacttggct aagaaacaag
    28441 aagaaaaaaa gaaacaaata ccgcaacagc ggacaaaaaa aggagtacta gcgggtatct
    28501 taggattggt tacgaatctg atcttgtttg tctctaagct aatgatcggg cttttttcag
    28561 gaagtgtctc cattatggct gatgcagtga acagtctatc agatactgct tcatccatat
    28621 tgactttgat cggatttaaa attgctgcaa agccagcaga tcaggaacat ccttatggac
    28681 acgaacgctt cgaatatatc agtgggttat ttgtttcgat catcattact tatgtaggat
    28741 ttcaattttt agagtcttct gctgaaaaaa tcatccatcc agaaaatgtc tctttaacac
    28801 ccatcgtctt ttttgtccta atcatttcca tctttttgaa atttgcacaa ggcaaaatgt
    28861 atcaggttat tgcgcaatcc attgattctg aaacattgag agctacagca acagatagtt
    28921 ataatgatgt ctttactaca ctggctgtat tgctttcagc agcaatcgaa tgggtaactg
    28981 gatggcgtat tgatggatat ataggttttg ctttagcatt atatatcctt tatagtggat
    29041 tgacgatgct tcgaaatttt gtatacgaac tattaggaag tcgcccaacg ccagcagaga
    29101 ttgaagaaat ggaagagcat ttaaatgctt gcgatgatat acttggctat catgatttat
    29161 tggtgcataa ctatggaccc aataaacgct ttgcatcagt ccacatcgaa gtagatgaat
    29221 caatgaattt gaatgtggct cataagatta ttgatctgat agaaaaagac tttaaaaagt
    29281 ctttggatgt ggaacttgtc tgtcacttgg atccaatgcc tgttgataac gaaaaatact
    29341 ataatatctt gcggcagtta aagcaaattc ttcttgaaac aggtacaggg cttaatattc
    29401 atgattttag agtaattcat caaggaaaaa cgctgcaatt tgatgtagtg gtccctgaga
    29461 aattccagtt tgttgataca gaactagaaa gaatcttacg tagtcaaata gaagaaaaaa
    29521 tcggagatta ccaattggaa attacgtttg atcacaatta tcttttatat taaatgagaa
    29581 actataaaaa gactccttaa agtttttaat ttaaagagtc tttttttctt ttaattgttt
    29641 atggagagcc tgacgtgcaa gattatctgc tcctttattt ttgttttcgg gcaaccattg
    29701 caggatcaat aaagaaaatt cggatagaag ttcttggatt ctttgaaggt agggaaggaa
    29761 gagctcgttc ttcgttgcgt ttttgtcgac cgtagaaact agtgttttgc tgtctgaaaa
    29821 acagaaaatc gtttcattct gtaagtcgtt tttctttaga aattctaaag tttttaaaaa
    29881 gacttcgaac tctgcttgat ggtttgtccc tatagaaagc gggatagata gctgttcttg
    29941 tttaccttga tagacaatca agattccacc tccactagga ccagggttgc cttttgtaga
    30001 ggcatcaatg tatactttta acataaaaag agattctcct ttttgttcaa actaatgtta
    30061 tggtaagatt atatcattac tgaatcggtg agaggttaga gaatgaaaaa aaatattcga
    30121 tggcagcctg aactggcaca tacgatcatc tattggtcat tgaccttgat cattctgttt
    30181 tttagtctga ttctttcttt agaaaacaca ggtccttatt ggaaaagcaa ttttgtttta
    30241 ggtatttttc ttgtttttct atttctaggt ttgcggcgtt cttttatttt aaaatcaaac
    30301 tctgtgatta tacgttatgc tgctttttgg aaagaccgag aaatatctgt cggacagatt
    30361 cgatctttga cgccaactaa tactggggtg caaatgatgt tgcaagatga gaaagcccca
    30421 tctgttttca tgatgcgaaa aaaagatcaa gtagtccttt ttgatcattt tgaaaaaaac
    30481 ggacccaaat cagaatcaat tccttatcct tatacgaaaa aaagaaaaag ctaattttaa
    30541 aaatcagaac ctaaaaaaac gaatttttac aggaaaagaa aagagtgaga cgtacggttc
    30601 atcccgttta gcgtctcact ctatttgttt atctattcat gcatactagt atcggtagtc
    30661 tgaacgttgc ctttcttgac atgggcagct tgtgttcctc gattcccttc gatgatctca
    30721 aattcgacca cttgattttc ttccaaagtt ttgaatccct cttcttcaat cgcagtaaaa
    30781 tgtacaaaaa tttcttctgt gtcatcataa ctgataaagc cgtatccttt tttgttgtca
    30841 aaccatttta caattcctgt ggtcaaagca ctcacgacct ttctaaagtc tgatcatagc
    30901 cttcattata aagagtattt ataagtacgt caaataaatt aaaaacctgt aattttatgt
    30961 tttttttctg aataaaaaaa ggtataattg gcatatctta acaaagaaga tattttgttg
    31021 aataaagttc gtgaaaaggg gtaatcaaat gaagaatgat ctgcaaattt cacaagaaac
    31081 agagctagaa ccgattgttg aagtggctag taaattagat cttaaggaag acgatttgga
    31141 actgtatggg aaatataagg cgaaaatcaa tttttctgga atcaatcgat caaaggataa
    31201 cgctgagggg catttgatct tagttacttc catcaatcca acaccagctg gcgaaggaaa
    31261 gtcaacgatc actgtagggt taggagacgc gctcaataaa atcaataaaa aatctgttat
    31321 tgcattgcga gaaccctcac ttggtcctgt gatgggaatc aaaggtggtg ctgcaggcgg
    31381 aggatatgca caggtattgc ctatggaaga tatcaacttg cattttactg gtgacatgca
    31441 tgctatcaca acagctaata atgcattatc tgctctttta gataatcata ttcatcaagg
    31501 aaatgaattg aacatcgatg cccgccgagt gatttggaaa cgggtagtcg atctcaatga
    31561 tcgtgaactg cgtaaagtaa ttgttggctt gggtgggcca atccaaggtg ttccaagaga
    31621 agatgggttt gatatcacag ttgccagcga aatcatggct atcttgtgtc ttgcggcaga
    31681 ccttgaagat ttaaaggctc gtcttgcaag gatcgttgtc ggctatacat ttgaccgtca
    31741 gcctgtgact gctggagact tgaaagcaga aggcgctctg gcattactgc tgaaagatgc
    31801 aatcaagccg aatcttgtac aaacaattta cggaacacca gcttttgtcc acgggggacc
    31861 gtttgccaat atcgctcatg gctgcaatag tattttagct accaagacag cattgagatt
    31921 agcagattac gttgtcacag aggcaggatt tggtgcagat cttggcggag agaaattttt
    31981 agatatcaaa gtacctaact tgaaaaaagc gccggatgct gtggtgattg tcgccactgt
    32041 ccgtgcattg aaaatgcatg gcggaatgaa aaaagatgaa cttaaaaatg agaatcttga
    32101 tgctttgaaa gtaggttttg caaatttaaa gcgccatata cgtaatatgg aacaatatca
    32161 gctacctgtg atcgtcgcaa tcaatgaatt cgtaacagat acagacaatg aactcacact
    32221 gttggagcac ttgtgtgaaa atcaggggat tcttgccaag agagcgagtg tatgggcaga
    32281 tggagcagaa ggcggcgttg atttagcaaa agctgtagta cgattgattg atagaaaaga
    32341 agctaattac aagccattat accgcctaga agaaacgata caagaaaaga cagaaacaat
    32401 cgtcaagaaa atttacggag gaaatggtgt tgttttcagc aaaaaagcgc aaaaacaaat
    32461 agaagagttt acgaagaatg gatgggacaa actgccgatt tgtatggcaa aaacacagta
    32521 ttcattttct gatgatcctt cactgctagg tgcaccggaa gatttcacca tcacgatccg
    32581 cgaaatcatc ccaaaattag gggcaggatt tttagtagcc ttgactggag atgttatgac
    32641 gatgcctggt ttgcctaaaa aacctgctgc attgaatatg gacgtgacaa atgacggaga
    32701 agtgttggga ttgttttaag cttgattata atctaccatc gaaggagctg tattttgact
    32761 acagcttctt tttttagacg atagggaatt aattttgttt acactaggat cattcttttt
    32821 ctttattgta ttttgagcta tactaaagca taataggagg gtatgatgaa aacgacattt
    32881 ataagaaaaa ccggttttta cggtatgggt agctcgattc agctaagaat caacaatgaa
    32941 aaacgcagtg ttctcagtca tgaacaaaag ataacgcttg atctggaagt gccctttacg
    33001 atgcaggtca ccttttattg gctaaaaagc cccgtttata cagtgaaaga agcaaagcct
    33061 gcttatgtga tcacaatgaa ttacttgatt cttcaaatct atccgttttt atttctagca
    33121 actgggttgg gggccgtttt cctacaaaat ttatttttca gtttttttgc agttattgtg
    33181 ttgattgcgt tctttttcta tataaaaaac cgggcatatt tgatcaagga ggaaaattat
    33241 ggcaaactct gatctttttt taatcagttt caatcgaatc gaaaaatgga tgagagacga
    33301 gatgggaaat gcacgaaata tgggatttac cgaacttgtc agacgcctag ctcaaagaaa
    33361 acaattaatg atcagaaaat acgaagatga tcttttacag cttgctcagt taaggaatgc
    33421 gatcgtccat gatcggatcg cagtcgattt tgtcattgca gagccaaatg attggacagt
    33481 caagcgaatc cagctgatcg aacaagaact gattcagcct gaaaccgtgc tgcctcgatt
    33541 tgcaaaacgt gtaacaggtt ttgaacaaga tcttccgtta cttgaactgt taaagatagt
    33601 agcagaaaaa aggtattcac aatttccgtt atacaataaa ggaaaatttg tagcattgat
    33661 tactttacgt aatatcgggt tttggctagc aaaagaaagc caaaaaggtc cggttgattt
    33721 aacaaataaa cgagcgttgg atttgatcat ccaaaacgga aagtacacaa attatcattt
    33781 tgttccagct aaaacacata tttacgaggt tgaagcgatg tttcgtgagc aagggacttt
    33841 ggaagccgta cttatcacta aagatgggaa tcccgatggt aatctgctcg ggatcgtacg
    33901 acctcgtgat atttataaac aaatagaaaa ggattgattt tttgttaatt atttattgga
    33961 tattcagcgc tttgctagta ggagtggatc agttggtcaa atggtggatc actgataatc
    34021 tagcgcttgg agagacgaaa aatcttatac caggtatttt gtcactgaat catattcgaa
    34081 acactggagc cgcatggagt atgctagaag ggaagatgtg gttctttact atagtgacac
    34141 tcattgcagt cgttgtgatt ctcacattga tgattaaaaa tcgtgaaaaa ggtaatcgct
    34201 ggttcatgat tggattgagt ttgattctag ctggtgcgat tggcaatttt atcgaccgta
    34261 ttcgtcttgg ctatgtagta gatatgttcc agacagattt tatgagcttt ccgattttta
    34321 acgtagcaga tgtgacactt gtatgtggag tgatttgtat gttaatttat attattttag
    34381 atgaaaagga tcaaagaaaa aaatgacaga acttaaaacg acgattcacc atgaatctgg
    34441 taggatcgat aaagtattga ctaatttatt caccgattac agtcgttcgc aaattcagct
    34501 gtggctgaaa aatggagcgg tttctgtcaa tggacagcca gtcaaagcaa attacaaagt
    34561 aaaacaagga gacgagatta ctgtcttgat ccccgaacca gaaacattag atgtggtccc
    34621 agaaaatatc ccgttgacga tcgtttacga agatgaagat gtagcggtga taaacaaacc
    34681 tcaaggaatg gtcgttcatc cttcagcagg tcatacgcat ggcacaatgg tcaatgcgct
    34741 gatgtaccat atcaaggatc tttctacgat caacggcgtt attcgtccag gtattgttca
    34801 ccgaatcgac aaagatactt ccggactgct aatggttgcc aaaaatgacc aggcacacga
    34861 atcattagct cagcaattaa aagataagac ctctttgcgc aaatatgttg ctttagtcca
    34921 tggagagatt cctcacgaaa aaggaaccat caatgcgcca atcggccgtt cgaaggaaaa
    34981 ccggaaaatg caggcgataa gagaagatgg gaagtcggct gtcacacatt ttactgtatt
    35041 ggaacgtttc gaaggattta ctctggttga attaaaacta gaaaccggaa gaacacacca
    35101 gatccgtgtg catatgaagt atatcggtta tccgttagct ggagatccag tatacggacc
    35161 gaagaaaact ttgaaaggca atggtcagtt tctccatgcg caaatgttag gttttacgca
    35221 cccgactaca aacgaaaaga tgatctttga agcacctctt ccagaagttt ttgaaaaaac
    35281 tttagaaaaa ttaagaaaaa acattgcatt ttaatttaga taacggtata gtaattaaca
    35341 acgaaagcat aaaacttaat agaattcctt taagtttagt cccgtgaggc taagaagggt
    35401 gggagcaagg atacgtatgg tgcgccataa gtagattgct cattgacata gtatagctat
    35461 cagtgtatca gtatacgctc agtctaaaca ttcctcctgt cctcttaggg caggagtttt
    35521 tttatgcaaa aaagtagggg gaatgaaaat gcaagcaaaa gaagtagtag atcaggtaac
    35581 catgaagcga gcattgactc gaattactta tgagattatc gagagaaacc atagtatcca
    35641 agatatcgta ttggtaggta tcaaaacacg aggaatctac attgcatcaa gaattgccga
    35701 acgcctgaag cagctagaag atatcgatat tcctgtagga gaactggata tcactttata
    35761 ccgagatgac aaaaaagaaa atccagaaga accggaactt cattcttctg acatcccagt
    35821 ttccttagaa ggaaaagaag ttattttgat tgatgatgtt ttgtatacag gacgaacgat
    35881 tcgagcagca atggacgcag taatggattt tggcagacca agaaaaattt cattagcagt
    35941 cctggtcgat cgtgggcatc gagaattgcc aatccgtgca gattatgttg ggaagaacat
    36001 tccaacatct cgggcggaag agattttagt agagatgcaa gagcttgatg gacaagatcg
    36061 catcatgatt ttgaaagagg aagattagag gagagagaaa catgtcggag aaaaaaggat
    36121 ttcataatga tgacgtggtg ttagatatcc acgatcgccc aaccccagga cattgggtag
    36181 gattaagttt acaacacttg ttcacgatgt tcggagcaac cgtgttagtc cccatcttag
    36241 taggtatcga tccaggtatt gcgctagtca gttcaggact aggtacattg gtgtacttat
    36301 ttactactaa aggaaagata cctgcgtact taggaagcag cttcgctttc attgcagcga
    36361 tgcagctttt gatgaaaaca gatggttatc cagcgattgc acaaggagca gtcacaactg
    36421 gattagtcta tttgattgtt gcgctgatca tcaaaaagat cggttcagca tggctggata
    36481 aaatcttgcc tccaatcgtc gtcggtccag tcatcatggt tatcgggttg ggacttgcgg
    36541 caaatgcagc aacaaatgct atgtataatc agggcacgta tgatttcaag tatattgctg
    36601 tagcgctgat cacattagga ttaacaattt tcttcaacat gtggctaaga ggttttctcg
    36661 gattgattcc gattttacta ggtattatta gcggatacat cgtagccttg ctgtttggaa
    36721 tcgtggatac taagccaatc gtagacgctg cctggtttgc tgttcctaac tttgagattc
    36781 cctttgtaca atatgaacca aagctatatc ttggagcaat cacaacaatg gcacccatcg
    36841 cttttgtgac gatgacagag catatcggcc atcttatggt attgaataaa ctaactaaac
    36901 gtaatttctt tgaagaaccg ggtcttcata agacgttgac aggtgatgga ttagcacaaa
    36961 tcgtggcagc ctttgtcgga ggtcctcctg taacaagtta cggtgaaaac attggtgttc
    37021 tggctatcac acgtgtccat agcgtgtttg tgatcggtgg agctgcagta tttgcagtca
    37081 ttttaggatt cgtaggaaaa ttaagcgctt tgattttaag tatccctgga ccagtcatct
    37141 caggaatcag ttttatctta tttggagtaa ttgccgcaag tggaatgaag attttaatcg
    37201 agaacaaaat cgattttgat aaaaagaaaa atctgttgat tgcttcagtt atcttagtag
    37261 taggaattgg cggactcgtc tttgaagtag gcacatttac attgtcggca atggcattag
    37321 caactgtact tggcatcatt ttaaatctta tcttaccaga aacttcacga agcgaagaaa
    37381 actaaggagg aaacattatg atcatcaaat cagaacgtat cagcttaaag catttattga
    37441 ccgttgaggc acttactgat caagaagtta tgggcttgat ccgcagaggg caggaattca
    37501 agcaaggtgc taattggact ccgcaaaagc gccagtactt tgctacaaac ctctttttcg
    37561 aaaatagtac tcgcacacat aaaagcttcg atgtagcgga gaaaaaacta gggctagaag
    37621 tgattgaatt tgaagcaagc acaagctctg tccaaaaagg ggaaacacta tacgatacag
    37681 tactgacgat gtcagcatta ggcgtagacg tagcggttat ccgtcatggt gatgagaact
    37741 attatgacga actgatacaa agtaaaacaa tccagtgttc gattatcaat ggaggagacg
    37801 gaagcggaca gcatccaacc caatgcttgc ttgatttgat gaccatttat gaagaatttg
    37861 gtcattttga aggtctgaaa gttgccatcg taggtgatat cactcactct cgcgtagcta
    37921 aatccaatat gcagatgtta aaacgtctag gcgcacaagt attcttctca ggtcctagag
    37981 aatggtacga cgatgaattc gaagtgtacg gacattatat gccattggat gaactactag
    38041 atcaagttga tgtcatgatg atgcttcggg tgcaacacga acgccatgat ggaaaagaaa
    38101 gtttctcaaa agaaggctac catcaggaac atgggttaac agttgagcgt gcgaagaagt
    38161 tgcaaaaaca tgcaatcatt atgcacccag caccagttaa ccgtgatgta gaattagctg
    38221 attcattagt ggaaggatta cagtcgcgga tcgttcagca aatgagcaat ggagtattcg
    38281 tcagaatggc aatcttagaa gcagtgctaa atggaaaagc gtaattatag gaggatgaaa
    38341 aaatgaaaac actcattaaa aacggtcaga tcaatacacg aaaaaatatg acgacaccag
    38401 cagaaatttg gatagaagat gggcgaatca aagcaatcgg tacaggattc tcagaagccg
    38461 aatttgatga agtgtttgat gcaaagggac aattgatcac acctggttta gtggacgtcc
    38521 atgttcattt gagagagcca ggatttacat acaaggagac gatcgaagct gggactcgct
    38581 cagctgctcg tggcggtttc actactgtat gcgcgatgcc gaatttaaat cctgtacctg
    38641 atacggcaga aaagctgaga caagtatatg acatcattag aaaagatgca gttgtgaagg
    38701 tattgcagta tgcgccaatc acagaaaatc tgcgaagtga gaaattagtt gatcaagaag
    38761 cactgattga agaaggtgct tttgctttca caaatgacgg tgtaggtgtc caaacagctg
    38821 ggacgatgta tctggctatg aaagaagctg caaaaaacaa taaagcccta gtagcccata
    38881 cagaagatga atctttatta tttggcggcg ttatgcacgc tggcaagaag gcagaagaac
    38941 ttggtcttcc cgggatctta agcgtaaccg agtcttctca aattgctaga gatctgttgc
    39001 tagctgaagc aactggtgtt cattaccatg tttgccatgt ttctactaaa gaaagtgtcc
    39061 gtgtaatcag agacgcaaaa aaagcaggaa tccacgtaac agcagaagtt tcaccccatc
    39121 atcttatttt gatcgacgaa gatattcccg aagatttcgg cttttggaaa atgaacccgc
    39181 cgctaagagg acgggaggac agagaagctt tgatcgaagg cttgcttgac ggtacgatcg
    39241 attgcatcgc tacagaccat gccccccatg gattagaaga aaaaagccaa agtttcatga
    39301 aatcaccatt tggtatcgta ggaagcgaaa cagcgtttca actgatctat actcattttg
    39361 tagaaactgg tcgattcacg ttggaacaag tgatcaattg gttggcagtt aaaccggctg
    39421 agatttttgg tttgaatgca ggaacgctaa cggttggtgc ccccgctgat gtagcagttt
    39481 ttgatattac acaaacttgc acgatcgata aagaagactt tttatcaaaa ggggagaata
    39541 caccttttat cggctggaaa gtaaaaggcg aaacacaaat gacttttgtc aacggaaaac
    39601 ttgtgtggca gaagggagaa taaaagtatg gaacgtttgc ttatattaga agatggaaca
    39661 gttttcgaag gaaaggcttt tggtgcagat gctagtgtct tcggagaaat cgtttttaca
    39721 acaagtatga ctggttatca agaaactatc actgatcaaa gttttaacgg tcaaatcatt
    39781 acatttacct atcctatggt gggtaattat ggggtaaata gagatgatta tgaatcgatt
    39841 tctccaacat gcaaaggagt agtagtaaaa gaacatgcac gtgtagcttc aaactggcga
    39901 aaccaaatga cgctagattc atttttgaaa caaaaaggaa ttcctggaat atcaggtatc
    39961 gatacgcgcg cccttactcg caaaatccgt gaagtcggaa cgatgaaagc tagtatagtt
    40021 gatgctggag attccttcga acatgcattt gatcagctga aagcaaccgt tctagcaacc
    40081 aatcaagtgc agcaagtctc aactaacaaa ccttatccaa gtccaggaac aggtcggaaa
    40141 gtcattgtca ttgacttcgg cttgaaacat agtattttac gagaattgag taaaagaaac
    40201 tgtaacctca cagtacttcc ctataacaca gatgccgaga cgattttagc gctatcacca
    40261 gacggagtca tgctgacgaa tggacctggt gatccaaaag atgtgccaga agcaatcgaa
    40321 atgatcaaaa aaatccaagg aaaagtaccg atttttggta tttgtctcgg tcaccagtta
    40381 tttgctttag ccaacggtgc agatacctat aaaatgaaat tcggacatcg aggattaaat
    40441 cacccggtac gagaagtagc gaccggaaga attgatttta cttctcaaaa tcatggctat
    40501 gcagtcgatg aagccacgat tgatccagaa aaactcatcg taactcatgt agaagtaaat
    40561 gacggaacag tagaaggagt caaacacaga gattatcccg ctttcagtgt gcagtttcat
    40621 ccagatgctg cccctggtcc gcatgatgca ctacatttat ttgatgaatt tatggaaatg
    40681 atggacgcag gaaaggagca gtaaacagat gccgaaacgt acagatatca agaaaattat
    40741 ggtgattgga tctggtccga tcatcattgg tcaagccgca gaatttgact acgcaggaac
    40801 acaagcctgc cttgccttaa aagaagaagg atatgaagtg gttttagtaa attctaatcc
    40861 tgcgacgatc atgacggata aagaagtggc agatcaagta tatatcgaac cgatcacact
    40921 tgaatttgta tcaaggattc ttcgaaaaga gcgtccagat gctctccttc caactttagg
    40981 cggacagaca ggcctcaata tggcaatgga gctggcagca tcaggtattt tagaagaatt
    41041 gaacatagaa cttctaggaa caaagctttc tgcgatcgat caagcagaag atcgtgatct
    41101 attcaaacag ttgatgaaag aattgaatca gccaatcccc gagtctgaaa tcgtcaatac
    41161 agtagaagaa gctgtagatt ttgcaaataa aatcggctat cctgtcatcg ttcgtccagc
    41221 ctttacttta ggcggaactg gtggaggtat gtgtgaaaat gaagaagaat tacgtatcgt
    41281 atcagaaaat ggattaagtc tttcccccgc tacccaatgt ttgattgaac gaagtattgc
    41341 aggttttaaa gaaattgaat atgaagtgat gagagattcg gctgataatg caatcgttgt
    41401 ctgcaatatg gaaaattttg acccggtggg tatccataca ggtgattcta tcgtatttgc
    41461 ccctagtcaa acattgtctg accatgaata tcaaatgctt cgtgatgctt ctctttcgat
    41521 tattcgagca cttaagatcg agggaggctg taatgtccaa ctcgctcttg atccacatag
    41581 ttttaattat tatgtgattg aagtcaatcc gcgtgtttct cgttcatctg cgttagcaag
    41641 taaggcgact ggctatccga tcgctaagct agctgctaaa atagcgcttg gattaacatt
    41701 agacgaaatg aaaaatcctg ttacaggaac gacttttgca gaatttgaac ctgcattaga
    41761 ttatgttgta gctaaaattc ctcgctggcc gtttgataag tttgaaaacg gagaacggct
    41821 tttaggcacg cagatgaaag caactggtga agtgatggct atcggtcgga atatcgaaga
    41881 gtctttattg aaagctgtgc gttctttaga aatcggtaca caccatatcg aacttcctga
    41941 gttggcagac atcacggacg atgacctaat ccaaaaagtg gttcgagcac aagatgatcg
    42001 cctgttttat gtagctgaag ctattagaag agggtacacc atagaagaat tagcggattt
    42061 gacaaaaatc gatttattct ttttagataa actgttgcat atcgtagaaa tcgaacagca
    42121 gttgaaacag cagccgttgg atgacaagat attgaaaaca gcgaaacaaa acggttttac
    42181 agacaaaaaa gtggcggagc tgtgggaaat aaacgaagaa gccgttcgcc agatgagaaa
    42241 agaaaatgat ttgattcctg tatataagat ggttgacact tgtgcagcgg aattcgaatc
    42301 aaaaacacct tatttttata gttcctatga atatgaaaat gaaagttcac gatcagaaaa
    42361 gccctcaatt ctagtactgg gatctggtcc tatccgtatc ggacaagggg ttgaatttga
    42421 ctatgcgact gtgcattctg tcaaagcaat ccagcaggca ggatacgaag caatcatcat
    42481 gaacagcaat cctgaaacag tttcgactga cttttctatt tcggataaat tatattttga
    42541 gccattgacc ttcgaagatg tgatgaatgt gatcgagcta gaacagccga taggagtaat
    42601 cgttcaattt ggtggtcaga cagcaatcaa cttagccgaa cctttgactg ctgctggtgt
    42661 aaaaatctta gggacaacga tcgaagactt ggatcgtgcg gaaaatcgtg atttattcga
    42721 gcaagcgctg aaagagttgg atattcctca acctttaggg gatactgcta caagtaaaga
    42781 agaagccgtg gttatcgctt cgagaattgg ttatccagtt cttgttcgtc caagctatgt
    42841 attaggcgga cgtgcgatgg aaatcgttga aaaccagcga gatttagaag attacatgga
    42901 acatgctgtg aaagcatctc cagaacatcc agtactagtg gaccgttact tgcttggtgg
    42961 tgaatgcgaa gtggatgcga tttgtgacgg tgaaacagta ctcatcccag ggatcatgga
    43021 gcatattgag cgtgctggtg tccattcagg ggattcaatg gctgtgtatc cacctcaaac
    43081 attatctgaa tcgatcaaga aaacgatcga agattacacg atccgcttag cattaggttt
    43141 gaattgtgtc gggatgatga atatccagtt tgtcatccat gaaaatgagg tttatgtgat
    43201 cgaagtcaat cctcgagcaa gtcggactgt tccattctta agtaaggtga caggaattcc
    43261 aatggcacaa attgcgacga aagctatttt gggagagaaa ctaaagacac ttggttatga
    43321 aaacggactt tatccagaaa caaaagaagt tcatgttaaa gcacctgtct tttcttttac
    43381 gaaattgcag aaagttgata cttacttagg gcctgaaatg aaatcgactg gagaagtaat
    43441 gggatctgac agacaattag acaaagcttt gtacaaggca ttcgaagctt ctggattaca
    43501 cttgcctgaa tatggagccg tattatttac gattgcggac gaaacaaaag aagaagcact
    43561 agaattagct aaacgtttta cagaaattgg ctatagtctg ataggaacga aacatacagc
    43621 tgcttatttc gaaaaaaatg gtctggtcgt tgaaacagta gcgaaaattt ctgaggaagc
    43681 agcagaaaaa aatgtagtcg acttaataag agatggtaaa acacaagtgg ttgtcaatac
    43741 gattgataaa gatcggggaa atgcttctaa agacggtttc atcatccgaa gagaagcagt
    43801 tgaacatggt attccattgt ttacttcttt agacacagca gatgcgatcg ttcgagtgat
    43861 ggaatcccgt gctttttcaa ctaaccctat ttaacggcga gcacatgtta agacaaaaag
    43921 acgaaaaacc gttgcaggag gggacagatg aaacaagaaa tgatggaaat cgttcagcaa
    43981 aaacagctgg caccaaagat ttttgaaatg acattgaaag gtgatctagt aaaagaaatg
    44041 cagcaaccag gacagttctt gcatattctc gtgccgcgtg cagacttgct attacgccga
    44101 ccgattagct tgaatcatat cgaccacgaa acaaatactt gccgtatcat ttatcgggtg
    44161 gaaggtgagg ggactagagt cttctctact ctttctgccg gagaccattt agatgtcatg
    44221 ggtcctttag gcaatggttt tgatttgtcc aatcttcata caggagacga agtatttata
    44281 atcggtggag gaatcggtgt ccctcctcta tatgagttat caaaacagtt agtcgaaaaa
    44341 ggaattaagc caacgcattt tttaggtttt gccacacatg aagtcgttta ttatgaaaaa
    44401 gaatttaatc gtctaggaga aacaagaatc gctactgacg atggaacata cggtatgcac
    44461 ggaaatgtcg gtaatttatt gttaggaacg aagacggaac ctacagctgt ttttgcatgt
    44521 ggttcaaacg ggttattgaa gacagtggaa cagctgtttt cttcgcatca aaatgtccag
    44581 ttatcattag aatcccgcat ggcatgtggg ataggggctt gctatgcttg cgtttgtcat
    44641 acgccggaag atgaaacagg aacaaaaagt gtgaaagttt gtgacgaagg accgattttt
    44701 aaaataggag aggtaattat atgaatgaga atcctttagc ggtcaaactt cctggcctag
    44761 aattaaaaaa tcctatcatg ccggcaagcg gctgtttcgg ttttggcaaa gaatacggaa
    44821 aatactatga tttgaatcag cttggatcga tcatggtcaa ggcaacgact ccaaatgccc
    44881 gctttggcaa tcccacgcca agagtggcgg aaacaccatc tggcatgttg aatgcaattg
    44941 gtttgcaaaa tcctggtcta gacgttgtta tgcataaact tcttccagaa ttagaggagt
    45001 acaatgagtt acccatcatc gcaaatgttg ctggtgcttg tgaagaagac tatgtagaag
    45061 tttgtagcaa aattggcgaa gcaccaaatg tgaaagcaat cgagctgaac atttcttgcc
    45121 caaatgtcaa acatggcgga attgcttttg gaacagattc tgatgttgct tttcaattaa
    45181 cacaagcagt aaagaaagtt tcttctgttc ctgtctatgt caaattgtcc ccaaatgtaa
    45241 cagacatcgt tccaatcgct caggcaatcg aagctggagg ggcagatggg tttacgatga
    45301 tcaatactct tcttggtatg cggattgacc ttaagacaag aaaaccgatt ttagccaatc
    45361 aaacaggtgg actctccgga ccagcaatca agcctgtcgc tattcgtttg attcaccaag
    45421 tagcaagtat atccagcctt ccaatcattg ggatgggcgg cgtgcagaca gtggatgatg
    45481 ttcttgagat gtttatggct ggagcaagtg ctgtagctat agggaccgcg aatttcaccg
    45541 atccgtatat ctgtcctaaa ctaattaaag agctccctgt gcgaatgagc gaactaggca
    45601 tcgaatcgct ggaaagatta atcaaagaag tgagagagga acgtgaacga tgaatcaact
    45661 agaccaacga ccaattattg cattagattt ttcaactagg caagaggtag aagactttct
    45721 tcgatttttc ccgaaagaag aaaaattgtt tgtgaaaatc ggaatggaat tgttctacca
    45781 agaaggacct gaaatcgtac gttatctaaa agaggctggt cataatgtat ttttagatct
    45841 gaaacttcac gatattccga atacggtcga aaaagcaatg cggggattgg caaagcttgg
    45901 tgttgatctc acctgtgttc atgccgctgg cggtattcgt atgatggagg cggcaatgcg
    45961 aggattagaa gaggggactc cagaaggtgg aaaacgtccg ctacttctag ctatcactca
    46021 gcttacttct acaagtgaag aagaaatgca tgccgatcaa ctaatcgaag tttctttaga
    46081 aaaaagtgtg atccattatg ctagctgtgc caaaaaagca ggattagatg gtgtggtttc
    46141 ttctgcttgg gaagtagaag cgatcaaaga aatggcagga gacgaatttg tttgcttaac
    46201 tccgggtata agaccagaag gaacagtcgc aggagatcag acaagagtag tcacaccttc
    46261 tcaggcaaaa aagatcggct caacttttat cgttataggt cgaccaatca ctcagtcaac
    46321 taatccttat aaagcatatc aaacgattca aacagaatgg agctaaccta aaatgaatgt
    46381 tgaacaaagt atcgcaaaag acttattaga aattgaagca gtatttttaa atccaagtaa
    46441 tccttttaca tgggcaagtg gaatcaaaag cccaatttac tgtgataacc gaatcacgat
    46501 gagctatcca aaagtccgaa aagagatcgc aaaaggatta gcaagcaaaa tcaaagaagc
    46561 ttaccctgaa gtacaagtga ttgctggaac tgcaacagca ggaatccctc atgccgcatg
    46621 ggtagcggaa atactggatt tgccaatggt ctatattcga agtaaggcga aagatcatgg
    46681 gaaaggtaac caaatcgaag gacgtatatt tgaaggtcag aagatggttg tgatcgagga
    46741 tttgatttct acaggcggaa gtgtcttgga agcagctgaa gcagcaaaaa gagaaggtgc
    46801 ggatatcctt ggtgttgcgg ctatctttac gtatgagttg ccaaaaggaa aagcaaattt
    46861 cgaaaaagcc gaaatccctc ttatgacgtt gaccaattat tctgtattga ttgaagcagc
    46921 tttagaagat cgttatatcg atgagcaaga attaactctg ctaaaagaat ggaaaaaaga
    46981 tcctgaaaat tggcagattg aagcgcgctt gatgaagata gagaaatccg atttgtgtct
    47041 ccagaatcat caattggaat agcaaaaatc caaaaactga ttgcgagaag catcttttca
    47101 tcaggtatga aaagaaactt ctatcgtgat cagtttttga tttttttaaa attcattttt
    47161 ctaagactgg ttagcagaaa gaagagaata atctcttctc ttttttctat tatatataga
    47221 ttttctctat tttttggtaa gggagctgtt tcaaaaatgg taaagagcgg aaatgttgcg
    47281 aatatcagtt atttaatgat atgatattcg gaaaaagaaa tctcaaaggg aggtaacttt
    47341 tatggggttg aatgaattta ttttaggatt gaacaattta gagacgatca caagggcacc
    47401 tggatttttt aaatatacag agcatacagt tgcagcacat tcgtatcgtg tagcatctat
    47461 tgcgcaagtt ctaggagaca ttgaagaagc gaatggacaa ccgatcgatt ggaaactgct
    47521 ctatgaaaaa tcattgaatc atgactacac agagcgtttt attggtgata tcaaaacgcc
    47581 ggtgaaatac gcaagcaaag agttaagagg gatgctgcaa acagttgaag aaaagatgac
    47641 ggacgaattc atcaaccaag agattccagc tgagttccaa gaagtttatc gccgtcggtt
    47701 gttcgaagga aaagatgaaa ccgtagaagg agaactatta gccatttcag ataaggttga
    47761 ccttctttat gaatcttttg atgaaatcat caaaaataac ccggaaaaag tttataagga
    47821 gatgttcatt gaggcagtga ctacgattaa aagctttgaa catcggcctt ctgttcagta
    47881 tttctttaaa gagatatttc cggaactatt gaaccaggaa ttttatggaa aagaggcgtt
    47941 tttagaaacc gtctcaacga ttgtaaagaa agaagaaaaa taaaaatgat atgagatgca
    48001 gggggaagta tagttgaaaa agaatatgga tgtcgaatgg ggctattctg gagacaaagg
    48061 tccggaaaat tgggcgagtt tatgtgattg gtttgcgaga ggtgcggaat ttccattgca
    48121 gtcgcctatt catttgacca agggggaaga aaccaaaata gaaggagaca cgctttcctt
    48181 tcattatcaa aagcaacggt ttactgacaa ggaattcaaa aacacgattc atttagtacc
    48241 ttttgaccaa ctcagttatg tagagttcaa gaaagaacgc tattatttga cagatattca
    48301 tttccacttg ccttctgaac atatcatcaa cggaaaacag gaagatattg aatttcattt
    48361 tgttcatatg aacagcaaaa aagagaatct agttgtgggt gtcatgtacc agctaatggt
    48421 acaggaaggt tggctgtata atcaagaaaa tggggaaagt tggaacttag aaaaacatga
    48481 gcattgggta aatccggatg tgttctttcc ggagcaaaaa agccatttcc attatattgg
    48541 ctcattgaca actccgccta ccagcgggcc aatccaatgg tttgtaatgg atcatgtagg
    48601 aaaactgaac caagagtttc ttctcccctt cgatggtcag tatgctcgac cgaacaaccg
    48661 accaatccag cccttgaatg gaagagaaat ttattatttc gaggagtttg agcaatgaat
    48721 attcagcaga tgaaatacgt ggtcgctgta gcaaacaatg gtagtttcag agaagcggca
    48781 aagaaaatgt ttgtctccca gccaagtctt tcagctggta tcaaagaatt agaaaccgag
    48841 ttagggatca cgctgtttac tcgaacgaat cgaggggcat atttaacaga agaagggcaa
    48901 gacttcttaa atcgtgccga gaaaatattg gtccagcttg atacattaga aactcactat
    48961 ctaacgagtg aaaagagaga caatttttcg atcgctgccc agcattatga tttcttaggt
    49021 cctttgaccg cacagctact cgaggaattc aaagacgaga tcaaacaatt tcggatcatg
    49081 gagacgacaa cagcgagagt gatcgaagaa gtcaaagagc agcacagtga gatcggcatt
    49141 atttatatga atgagcataa tcaagctggt atcagaagat atttacaaca aggagagcta
    49201 agtggccgca gcattggaaa attcaaaacg catatttttt taaggcaagg acatccatta
    49261 gctgaacacg cagagatcac caaagaagaa ttgttatctt atcctcaagt tcgttttaca
    49321 caggatggaa ataatttccc gtatttttat gaagacctta tcgagatccc tgatcaagaa
    49381 acagtgattt atacgagtga tcgaggtact ttgatgaata tcgttcttga aactgatgct
    49441 tacgcttcag gatctggtat tgtgattgga gggattcgcg agcacttgcg gttaatccct
    49501 ttggcagaag gcgaactaaa tgaattttat atcatttatt cggcgaaacg tccgctcagt
    49561 gaaatcgctc aaagatttat caaagagctt actcggttac tggaccagca gagctaagaa
    49621 aagcagatga gaacgctgtt tattgaacac cgttgatcaa aaaagagggg ttgtgacaga
    49681 attttgtcac aactcctctt tttgtagcaa acaatttata actaattgct gttgtgggaa
    49741 agtacgcaaa tatgattcta gaaacactca ttgattacct gttttgtttc atagctgtaa
    49801 ttttttcttc ttctggtgtc acgataatcg tcttttggtt ttcataaata acataaccag
    49861 gcttggcgcc atttggtttt cggatatgct ttacctgtac aagatcaacc ggcacttggg
    49921 cagagtatcg gtattttgaa aaataggcag ctaactcggc agcttctgta attgtttcat
    49981 ctgagggctt gtcactttta atgatgacgt gcgaaccggg tatatttttt gcatgaagcc
    50041 aatagtcggt ttttttagct gtcttcattg tcagctggtc attttgaaga ttgtttttac
    50101 caacaagaat gagcgttccg tcagaagaaa agtattgatc aggctgagat ggtttcttac
    50161 gtttttgttt ttttaagttc ttctttttca ggtatccttc tgaagtcaat tcttctttaa
    50221 ttgcttcgat atccattggg ccggcaattt ccagttgaga taagacagat tcaagatatt
    50281 ggatttcatc tttggcttct tggatttgtt cgccaatcag cttaacagcg ttcttcagct
    50341 tttggtatcg atggaaatat ttctgtgcat tttgatttgg tgtcaatgcc ggatctagtg
    50401 ctattttgat tggccgatct tcttcataat aattaggtaa aacaacttct tttgcgccgc
    50461 gtggtacttg tgtcagaaaa gtagtaagta gttcgccatt tctacggtaa ttctcagcgt
    50521 tttcggattc tttgagtgtt tgttcacgtt ttttcaattt atttttattt cgttttagtt
    50581 cattttcgat ttttcgaatc agttctccgc cttgttgttt tgcccgatct ttctctgctt
    50641 tttcgtgata atagctatct aataattgac tgagtgttgg ataagctgtt acagcagatg
    50701 cctgttcgga caatacctga taagcaatcg gtgtgaaaaa atctttattt tccgtttcgt
    50761 aaaaagtagg aataggctga tggctgatgg ctgagaaaaa ttcgtcccat acaaccatct
    50821 tttcattagg acgttccagt aatcttttgg tcagctcttg agcgatatcg aaaccgattc
    50881 cctgaaactg acgttggatt gcttttggat ctagttcagt ttgggaaagc cgctgaaaga
    50941 ttttttcttt ctcggagcta aatgggttca agacctcttg ttttggcgga gcaacatatt
    51001 ctacaccagg aagcaaagaa cgataagtgt tttgagagct tccaatgtgt ttgatcgcat
    51061 ccagtatttt tccagtttct cgattaacta agacgatcgt actatgacgt cccattaatt
    51121 caacgatcaa tatgatattt tgtagatcac ccaactcgtt tcgtttagca aagtggaaat
    51181 ggatcactcg atcattttcg atttgttcga tgtcttctaa aatagcgcca tccaaatatt
    51241 ttcgaagcat catcacgaag ttaggcggat tatcaggatt ctgataatcg atttgggtaa
    51301 tctgtacacg agcataactt gggtgcgcag ataataataa tcgatggttt ttgccgcgtg
    51361 aacgaatcac cagcacgatt tcattttcat aaggctgatg gattttcgag atccggccag
    51421 atagtaacgt ttcacgcaat tcgttgacca tcgcgtgggt aaatacacca tcaaaggaca
    51481 tggttcattc ctcatttctt ttcataagta tattcattat aacgaaaaaa agaagaaaaa
    51541 ccaatttggc cgaatagtaa agcaaaagtc tagaaaaatc ttggactaat tacataaaaa
    51601 acagtgtttc tatcgttgaa atccaaattt ttagg
//
LOCUS       DACDXE010000014        46827 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.014, whole genome shotgun sequence.
ACCESSION   DACDXE010000014 DACDXE010000000
VERSION     DACDXE010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.014"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            415..1548
                     /locus_tag="IWM23_000806"
     CDS             415..1548
                     /locus_tag="IWM23_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAP9883471.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            1579..1899
                     /locus_tag="IWM23_000807"
     CDS             1579..1899
                     /locus_tag="IWM23_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883472.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(1928..2392)
                     /locus_tag="IWM23_000808"
     CDS             complement(1928..2392)
                     /locus_tag="IWM23_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883473.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(2524..3777)
                     /locus_tag="IWM23_000809"
     CDS             complement(2524..3777)
                     /locus_tag="IWM23_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883474.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(3806..4123)
                     /locus_tag="IWM23_000810"
     CDS             complement(3806..4123)
                     /locus_tag="IWM23_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9883475.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(4149..4469)
                     /locus_tag="IWM23_000811"
     CDS             complement(4149..4469)
                     /locus_tag="IWM23_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883476.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(4933..6348)
                     /locus_tag="IWM23_000812"
     CDS             complement(4933..6348)
                     /locus_tag="IWM23_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9883477.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(6365..7681)
                     /locus_tag="IWM23_000813"
     CDS             complement(6365..7681)
                     /locus_tag="IWM23_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883478.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(7831..8529)
                     /locus_tag="IWM23_000814"
     CDS             complement(7831..8529)
                     /locus_tag="IWM23_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAP9883479.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(8818..9807)
                     /locus_tag="IWM23_000815"
     CDS             complement(8818..9807)
                     /locus_tag="IWM23_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HAP9883480.1"
                     /translation="MYIADSARYEKMEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQ
                     REILRGAFDMGITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMW
                     EGPYGEWGSRKSIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGK
                     ALYIGISNYNGEDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGA
                     ITFSPLAQGLLTNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQS
                     LAQMALAWNLRQKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(9887..10720)
                     /gene="msrA"
                     /locus_tag="IWM23_000816"
     CDS             complement(9887..10720)
                     /gene="msrA"
                     /locus_tag="IWM23_000816"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9883481.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(10895..12529)
                     /locus_tag="IWM23_000817"
     CDS             complement(10895..12529)
                     /locus_tag="IWM23_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883482.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(12516..14363)
                     /locus_tag="IWM23_000818"
     CDS             complement(12516..14363)
                     /locus_tag="IWM23_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883483.1"
                     /translation="MFFFIFFSYLKVIKIDSFLVYHTSAMKNLTQNLLKKDNSSILKI
                     ELKKENNKSMKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRIKGLSS
                     IFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNICTILI
                     SFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYYAYGLI
                     FNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFVVVNLV
                     KAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDITTYTM
                     GLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLLQFPWR
                     ILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVIQSKHG
                     HIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKAADNNI
                     TYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIEITTKY
                     TPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
     gene            14350..15987
                     /locus_tag="IWM23_000819"
     CDS             14350..15987
                     /locus_tag="IWM23_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAP9883484.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(16132..16872)
                     /gene="truA"
                     /locus_tag="IWM23_000820"
     CDS             complement(16132..16872)
                     /gene="truA"
                     /locus_tag="IWM23_000820"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAP9883485.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(17063..17698)
                     /locus_tag="IWM23_000821"
     CDS             complement(17063..17698)
                     /locus_tag="IWM23_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9883486.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(17845..19086)
                     /gene="sstT"
                     /locus_tag="IWM23_000822"
     CDS             complement(17845..19086)
                     /gene="sstT"
                     /locus_tag="IWM23_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAP9883487.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            19284..20282
                     /locus_tag="IWM23_000823"
     CDS             19284..20282
                     /locus_tag="IWM23_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883488.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            20295..22313
                     /locus_tag="IWM23_000824"
     CDS             20295..22313
                     /locus_tag="IWM23_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HAP9883489.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(22373..23089)
                     /locus_tag="IWM23_000825"
     CDS             complement(22373..23089)
                     /locus_tag="IWM23_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9883490.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(23316..23858)
                     /locus_tag="IWM23_000826"
     CDS             complement(23316..23858)
                     /locus_tag="IWM23_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9883491.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(23863..24090)
                     /locus_tag="IWM23_000827"
     CDS             complement(23863..24090)
                     /locus_tag="IWM23_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAP9883492.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(24169..26049)
                     /locus_tag="IWM23_000828"
     CDS             complement(24169..26049)
                     /locus_tag="IWM23_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9883493.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(26238..26852)
                     /locus_tag="IWM23_000829"
     CDS             complement(26238..26852)
                     /locus_tag="IWM23_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883494.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            26994..27554
                     /locus_tag="IWM23_000830"
     CDS             26994..27554
                     /locus_tag="IWM23_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9883495.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(27859..28173)
                     /locus_tag="IWM23_000831"
                     /pseudo
     CDS             complement(27859..28173)
                     /locus_tag="IWM23_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(28427..30062)
                     /locus_tag="IWM23_000832"
                     /pseudo
     CDS             complement(28427..30062)
                     /locus_tag="IWM23_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(30617..31180)
                     /locus_tag="IWM23_000833"
     CDS             complement(30617..31180)
                     /locus_tag="IWM23_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9883496.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(31195..32238)
                     /locus_tag="IWM23_000834"
     CDS             complement(31195..32238)
                     /locus_tag="IWM23_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883497.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(32371..32775)
                     /locus_tag="IWM23_000835"
     CDS             complement(32371..32775)
                     /locus_tag="IWM23_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9883498.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(33008..33643)
                     /locus_tag="IWM23_000836"
     CDS             complement(33008..33643)
                     /locus_tag="IWM23_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAP9883499.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(33909..34961)
                     /locus_tag="IWM23_000837"
     CDS             complement(33909..34961)
                     /locus_tag="IWM23_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883500.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(34974..35912)
                     /locus_tag="IWM23_000838"
     CDS             complement(34974..35912)
                     /locus_tag="IWM23_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9883501.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(36363..37013)
                     /locus_tag="IWM23_000839"
     CDS             complement(36363..37013)
                     /locus_tag="IWM23_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883502.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(37113..37622)
                     /locus_tag="IWM23_000840"
     CDS             complement(37113..37622)
                     /locus_tag="IWM23_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAP9883503.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(37823..38692)
                     /locus_tag="IWM23_000841"
     CDS             complement(37823..38692)
                     /locus_tag="IWM23_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9883504.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(38712..39833)
                     /locus_tag="IWM23_000842"
     CDS             complement(38712..39833)
                     /locus_tag="IWM23_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9883505.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(39835..40230)
                     /locus_tag="IWM23_000843"
     CDS             complement(39835..40230)
                     /locus_tag="IWM23_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9883506.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(40249..41046)
                     /locus_tag="IWM23_000844"
     CDS             complement(40249..41046)
                     /locus_tag="IWM23_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9883507.1"
                     /translation="MSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALSPIL
                     RKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKVGTM
                     GPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEFGYK
                     QGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQPMLD
                     KILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(41048..41836)
                     /locus_tag="IWM23_000845"
     CDS             complement(41048..41836)
                     /locus_tag="IWM23_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883508.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(41850..42326)
                     /locus_tag="IWM23_000846"
     CDS             complement(41850..42326)
                     /locus_tag="IWM23_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883509.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(42398..45055)
                     /locus_tag="IWM23_000847"
     CDS             complement(42398..45055)
                     /locus_tag="IWM23_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9883510.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(45074..45478)
                     /locus_tag="IWM23_000848"
     CDS             complement(45074..45478)
                     /locus_tag="IWM23_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
                     /protein_id="HAP9883511.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(45710..45967)
                     /locus_tag="IWM23_000849"
     CDS             complement(45710..45967)
                     /locus_tag="IWM23_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HAP9883512.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(46092..46424)
                     /locus_tag="IWM23_000850"
     CDS             complement(46092..46424)
                     /locus_tag="IWM23_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883513.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        14735 a         9267 c         7240 g        15585 t
ORIGIN      
        1 acaccgttta ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt
       61 gtgaaaatgg ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct
      121 tctgtcataa cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac
      181 gtttactctc ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat
      241 tacactaaaa aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt
      301 tcatttaaga aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt
      361 atctgtagta ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa
      421 gcagtcactt ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata
      481 ttagaaccaa cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg
      541 catctttacc atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca
      601 atgggaatcg ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg
      661 gttatccctt ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc
      721 caatgtgatg aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg
      781 aacggtaatc attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact
      841 tcatttgttg taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt
      901 ttgcccacag cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg
      961 attctaggtt ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca
     1021 gagcgcgtaa tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat
     1081 ggggttgaaa cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg
     1141 gctaaaggtg gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc
     1201 aaagaaaaag cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg
     1261 gcatcgcaag ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca
     1321 gcttcttctt acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag
     1381 gcaccaatta tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat
     1441 cctacacaaa tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc
     1501 tttgacaaaa aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa
     1561 atacaggtga ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga
     1621 aaagaagaag atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac
     1681 acattgatct cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt
     1741 gcttccttaa ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt
     1801 ttcttgacga ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa
     1861 aaagcaatga agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc
     1921 tcttttttca ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct
     1981 tgaccattgt caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta
     2041 agtaaagttt ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct
     2101 tggctatccc ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca
     2161 gatcagggcg gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt
     2221 tttcatctaa gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa
     2281 tactttcctc atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt
     2341 gttttactaa ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa
     2401 cctccgacta aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg
     2461 acaacaatag tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat
     2521 taatcaagca tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg
     2581 tagatagatc aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact
     2641 ttttgttgcc aatacaccgc caataattgg tggagttacc catggaggca taacggtagc
     2701 tgctggcact aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt
     2761 caaaataaat ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg
     2821 ttcattaata ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt
     2881 gtttttacgt cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc
     2941 taagttgaca aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc
     3001 tgcgattgca ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc
     3061 gaccatattc gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag
     3121 taaggcagat ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa
     3181 agaacctacg atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc
     3241 agagatagtg atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac
     3301 accatcaggc attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt
     3361 tgaaataatc ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc
     3421 gcctaaagcg aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta
     3481 gccgttagct aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt
     3541 accgttccaa gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat
     3601 caattcttgg aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat
     3661 cattaacggc atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc
     3721 aatttttgat gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa
     3781 tcttccctct tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct
     3841 cttttgctaa atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca
     3901 aaagcgtgac ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt
     3961 gatgtgcttg gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat
     4021 cacctttttt cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca
     4081 tgatcagacc catgatggct tctagatttt tttgatcttc catttattgt atcctccgat
     4141 aatttttatt atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc
     4201 atgccatagt cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca
     4261 gcaaattgat tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta
     4321 gattctagtt gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct
     4381 tttgcttctg ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa
     4441 catacaagca tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt
     4501 gtatataagc aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat
     4561 ttaaggtcta taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa
     4621 ttaattttta ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg
     4681 tcacaacttt tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac
     4741 caagttaaaa acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca
     4801 gctttttcac aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa
     4861 aacgctggat tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca
     4921 aaaaacttct atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa
     4981 agtctgatac cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat
     5041 tcgaactaat ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc
     5101 ccataacata tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa
     5161 atgctgtccg ataaattcga tacggtaatc atcttgcacg attcctttct catccatata
     5221 ctgttcttca tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata
     5281 attttctttt agatacatgg ctatatcata catgattttc ggatagattt cccatcccct
     5341 ggaattattc attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa
     5401 aggtgtctct tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt
     5461 gattccaagg aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc
     5521 gaaataaatc gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac
     5581 catcggatca aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc
     5641 ttctggagaa ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc
     5701 accataattt ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata
     5761 attccacatc atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata
     5821 gcaacgcgtt tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc
     5881 gtaacgatca aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga
     5941 ccagccatca tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat
     6001 cggttctacg tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc
     6061 ttcatctact atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg
     6121 ataatgtgtc agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa
     6181 attagttgct acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca
     6241 gctatctata aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc
     6301 agaagatgca gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg
     6361 ttttttagat cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca
     6421 aaatagaaac aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg
     6481 ccagataagc attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg
     6541 caaatcctaa agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa
     6601 acggaatagc taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga
     6661 tgttgaagat cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc
     6721 caactaacaa tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat
     6781 acatatccca aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt
     6841 ccgtatttac agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat
     6901 ttccatggat accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg
     6961 gtaacccttg tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa
     7021 ggtacattcc tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca
     7081 gaatcgtcgg gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca
     7141 ttctcagctg tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg
     7201 agaccaacat tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt
     7261 ctactataac agtctgattt tcattcacta attcataaac agttggatta acagataaat
     7321 aagacatcac tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt
     7381 cgatccctat cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg
     7441 cagatgcgat aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat
     7501 tttccgagtc aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg
     7561 gaatcaatgc agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg
     7621 ttgccacgtt ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca
     7681 ttcctgcttc ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg
     7741 atgaacagaa actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg
     7801 tatttatcat aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt
     7861 ctgtaattct ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc
     7921 attccgcctt tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag
     7981 atgctggata tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat
     8041 atgcagcaag gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa
     8101 ttgaacatgt tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt
     8161 catttggacg atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc
     8221 caataaatat ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga
     8281 aaaaccgagt gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg
     8341 ccgaacttca aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg
     8401 ccccacctct aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa
     8461 ctgcatttcc tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt
     8521 ttcttccaaa ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac
     8581 gtttgaatca tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca
     8641 aacgaagaag atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc
     8701 atttttattt tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc
     8761 ttgatgtcat cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta
     8821 ttctagaatt tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat
     8881 tcgaacactt tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga
     8941 tttttgtcgc aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc
     9001 gattgtttgc agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa
     9061 tggtatttcc ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt
     9121 cagcaaccct tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac
     9181 tggagataga tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat
     9241 aatgaacggt gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt
     9301 atagttggat ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc
     9361 tgctgtttct tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata
     9421 atctagcccc attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc
     9481 ccattcccca tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc
     9541 atcacggtag gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc
     9601 agcagggcct ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc
     9661 tcttaaaatc tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc
     9721 gagagataaa atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc
     9781 ataacgtgca ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg
     9841 ctttcttttt tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat
     9901 tttttcgtat atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac
     9961 gtgcttgctg gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt
    10021 tttctgctgg ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag
    10081 ttcctgaact gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt
    10141 gataaaaaat cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg
    10201 tgggatcagt aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt
    10261 cataaatgat ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat
    10321 ttggtttttc aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg
    10381 attcaaacgg ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat
    10441 agtcagcatc ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt
    10501 ccgtaatcaa taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg
    10561 gacgtagtgt tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt
    10621 cattctttgt ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca
    10681 gattataaag tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa
    10741 gaagacaagc tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact
    10801 tcatccccct acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt
    10861 tttaatcaat ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa
    10921 aaaataaaca taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat
    10981 aacttgtgct tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat
    11041 tagcattcac ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag
    11101 catatacagg aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac
    11161 taccagttgt tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta
    11221 ttatatgctc cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca
    11281 ttcctttagt ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag
    11341 aaattatttt ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa
    11401 cagtcataaa taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata
    11461 tcatcgttaa tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt
    11521 gtaacaattt ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta
    11581 gccaagtcaa acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata
    11641 aaatcagggg aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt
    11701 cgttgttcat agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg
    11761 tcacgctagg tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag
    11821 cactcgtcag aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa
    11881 aatctgcatt cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg
    11941 ctgtactcat taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta
    12001 gctttacaga cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata
    12061 cagggataac aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta
    12121 ctgtccgata agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt
    12181 ttaaaaaccg ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa
    12241 cgtaaataat atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa
    12301 aaaaagtcag aaagggataa aaataattta caccatatcc agtataattg aatgtctcat
    12361 aattaatcgg actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa
    12421 gatctccagt ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac
    12481 taactataat taatccactc aatattacta cttttttatt tattttcatt cttcttgttt
    12541 atctcctttt tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa
    12601 cttgaataca ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt
    12661 ggagtatatt ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact
    12721 aagccatgtt ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct
    12781 aaataagcat atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta
    12841 atgttattat ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca
    12901 tgtttttcta agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct
    12961 cccatatctg atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt
    13021 tttgattgga taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac
    13081 accacagtag cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa
    13141 acaagcgtta tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac
    13201 tggagtaagc ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat
    13261 acccatgtag aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt
    13321 atgaataaag gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata
    13381 tcgttattta acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct
    13441 gttttgaagg gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa
    13501 tagctagtta atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt
    13561 atatcgtttt ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat
    13621 gcactacaca atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat
    13681 aaagcgatga cttttttttc tctattgaat atcaatccat aagcataata taaaacagct
    13741 gggataatcg taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt
    13801 cgataagttg caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa
    13861 aacttttctc cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat
    13921 agaacataac ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt
    13981 gtaagaaaag gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg
    14041 attggccctt caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca
    14101 cctgttgtat aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt
    14161 aagataagaa ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc
    14221 ttttttaatt ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa
    14281 tttttcatag cactagtatg atatactaga aaagaatcaa tttttattac ttttaaatag
    14341 gagaaaaaaa tgaaaaaaaa caaaaaccaa ataatagata tcatttttat gttatttcta
    14401 agttttgctt acgtcatccc tctattcatg tctaagggaa tctatcatag tgtcaatcag
    14461 gatacttact ttcatttatc tcgaatcata ggactagata atgtatggag ttctccagta
    14521 aacttcaata acttcgatca tcatggaaca atgatgaata ttttttatcc atggcttact
    14581 ctttatccag cttttttatt ttataaaatg atgggaaatc ttgttttaag ttataatatc
    14641 tactattttt ttatcacttt tttaacaatg gtcgtttctt atttttctat gaaacaaata
    14701 aaaaacaatc gttatatttc gctgttattt tcgattattt atacattttc cgcctatcgt
    14761 gccattgaca tattccatcg tgcttcattg ggagaagctg tcgctttaac atttttgcct
    14821 ttgatactaa tggggtgtta cgaaatttat attagagatt accaaaaatg gtactggttg
    14881 tctattggca tgacgctagt agtgtatact catttacttt ctgtcgctat ggtttctgta
    14941 tttattggag gaacgttgtt tctttctttt tatttttggg accaaaaaat cgctagactc
    15001 ttaagtcttt taaaagctac cgtcctcacc ttttttttaa gtgcgggatt cttaattcct
    15061 tttattcaac aatctagagc acaagaattg aaagtcccac tgggaaaaga attaagtggt
    15121 atggctccta gtgatatgct gacacatatt ttaaataata actataacaa ctatactatt
    15181 ggattattct tatttctcgg attgattggt gcttttattt ttataaaaaa attaactgct
    15241 gatgatttat ttattttctt tcttggtgta tttgttcttt tttgttcaac caatttattt
    15301 ccttggcaac tttttaacca tacaccagtt aagagtctac aatttgtctg gcggttaaat
    15361 ggtttttctt ctttatttat agcctatacg atgagtatag tgatctatta tattttttct
    15421 actaaaaata attcatggaa aataatggtc tttacgtttc ttgcactaat attgcatctc
    15481 tcaggagtaa gcaattctat ccatgctaac aaagattcat taacacttat agcacctgaa
    15541 gacgctgttg ctatagctga aaattacaat catactgatt atgcaaacaa agagtcaatc
    15601 tatcatcctg atatgattaa taatgatcaa tatttactag gtaaccagga aataaatcca
    15661 acaactcatt ttatgagtaa caaacttaca attgaattag ataattcgaa taataaaaat
    15721 actgtcttga cgacacctat ttatcgttat aaagggcaag tggcttctat taatggcaaa
    15781 ctagtacaaa caaaattatc taagtttggc acaactgaat taactattcc tccaggtata
    15841 aataaagtcg ttattactta tcagtacaca aaactagcaa ttgcttcaag atatttatct
    15901 atcgttactt tgattttatt tttactttat cgatttactt tttctaaaca aaaacaaccg
    15961 agaagagaag tacaacattc tcactagatt tttatttaag aaagactttt tagctgtaca
    16021 cataaacagg ctaaaaagtc tttttttata tttctttaat ttgcttcata cattatttaa
    16081 catcgatatt tatactgtga aacatgcgtt tcatccatta tcaaagtatt tttatcgata
    16141 ataaacttca tccaaaaaca gaccttgtgc agggacggtt tctcctgcat gttgccgcac
    16201 tttgctttca aatatttcat cgattgtttc tatcggcatg gcacctgtgc cgatttctag
    16261 taatgtcccc atgataatcc gcaccatttt atataagaat ccgtctccga caaaagtaaa
    16321 atgaagcatt tctccctttc gttcgataga taattcatca atcgtacgca ctgtggattt
    16381 ttttgatttt ttcaatgcag aaaaaccaat gaaatcatgt gtacccacta atttttcgca
    16441 agctgcattc agcttgtcca tatccaactc ttgagggaaa taaaagctgt ggtttcgctc
    16501 aaatgctgaa ggaatcacgt tgttccaaac ataatagctg tattttttgc ccaccgcatt
    16561 atatcttgaa tggaagcgct caggcatttc ttcgattctt ttgatgacaa tatcccgcgg
    16621 aaggtaacga ttcataaagt cgatcatttc tgccagtcca atttctgtgt ttgttttaaa
    16681 atttgctact tgtcctcgcg catgggtgcc agcatctgtt cttcctgatc caataatttc
    16741 tatctcttca tttgtcatct gatgcaatac tttttcgatt tttccttgaa tggttttatc
    16801 gtcatctcca agtctttgcc atcctgaata tctttttccg tcatattcga ttgtcaactt
    16861 gatgtttctc atattttagt ggctcctctg tcttccattt ggtcttctct ctaccttttt
    16921 aagattgtag cacaaaatat agctccttgc ttctctaaga cagaagctat acaagaaaac
    16981 tgtggaaata tatatttcag aaaaacaaaa aacagccacc ggatgaagta tccgctgact
    17041 gttcaatttg acggctcaag ttttactgtt tgagatgcaa gatactcaac ctggcaatgg
    17101 gcacataatt aaagaaaaaa ctgattcttt ggcaaaaacc tattttattc agtaatggcc
    17161 aagaatttat agaaggaatt cttgccagtc cataaatcgc cgcaaaaagc aaactgatca
    17221 cagacagtcc aaggtataag tggcttacgt ccatttgccc attttttcga tagatcaaga
    17281 aaagaagaac tggaaacaat aaaaaaccac tgtaacctaa acctgatccg atattatgga
    17341 cccatgtcga gacattccac gaagattcat gggtattgat actgaacaaa cctgtaaaga
    17401 tacaatcacc tataccatag aacgcgatgg ctgctgtaac tagggtagct aacacacgag
    17461 atgtactaac aaatgcagca tacaatgcag gcaatgacag gatgaaaaag atccctgaaa
    17521 ccacagacca aataagaaac gcttttctta caggactgga aacctctcca aatacactga
    17581 tcacgctctt catttgatct aattcaggat aatacaatcc taatacgtac ggagtcaaaa
    17641 aatcactcag cacgcctaat aataaaaaga aaaaaccaaa tttacgtaaa aaatccatga
    17701 tacgcctcca ataacaatta cctatgcttt taattgttat tataaacgac tttatcttga
    17761 tttaacagaa acctctattt acacgaacat ctatataact cattaaaaac tcgaatcatt
    17821 ttaaaaatac taaatatcta acgttcaacg ttcacttaat ccatctaaac aatctttcaa
    17881 tttgattttt tccccacttc ttttacgcgc gcctagctct cctattgcag taaacaacag
    17941 atcacttgac gaatttaagg cagtttccat tgaatcctgg ataacaccga taataaatcc
    18001 aataccgacg atttgcatcg cgacatcatt agagatacca aacaaactac atgctaatgg
    18061 aatcaataac agtgaaccgc ccgcaacacc tgaagctcca caagcagcta aagcagacaa
    18121 aatacataaa aggactttca aaacaaaagg tgcttcaatc cctaaagaat gtacagcagt
    18181 caacgtcatc aaggaaatgg taatcgcggc accgcccata ttgatcgtag cacctaacgg
    18241 gatcgatatg gaataagact cttttgtcaa tttcaatctt tcagccaaca tcatgtttat
    18301 cgggatattt gcggcagaac ttcgggtaaa gaaagcggga agtgcgcttt ctttcaaaca
    18361 aaagaaaatc aatggatagg gattttcttt aataaacaaa aacgtgagga atgggtacac
    18421 aactaatgcg acaaacagca tcgtaccgat caaaacaagt aaaagctgca gatactctgc
    18481 caatccagcg attccagtcg tagcaattga ttgataaacc aatcctaaaa tccctatcgg
    18541 agcaaatgca ataatcactt gtacgacttg tgacaacgct atagatatct gatccaccaa
    18601 ctcttttgtc gattctgaac gtgctcgaaa agctagtccc aacaatacag cccagaataa
    18661 gacgccaagg taattcgcat caataagaga ttgaacagga ttttgtgtga cattcgtcaa
    18721 catagtagat aaaacacttt ccaaactttg cggtgctttt tcagatactg tttcttgtaa
    18781 agtaatagga acagtaaaaa ttgaacttac aataactgct acaagtgcag aaagcaatgt
    18841 tcccactaaa tataagatca aaataggctt cacgaatgtc ttattcccaa ttttatgctt
    18901 ggcaattgac gccatcgtca agaaaaaaac cagaataggt gcgattgcct tcaagcaact
    18961 gacaaacaac gttcccaata ttgaaataaa tgtccatgtg gggaaaaaga taccaaacaa
    19021 tattccgact aaaataccaa caacaatacg ttgaactaac gacatatcca aaaacttctc
    19081 taacaaatga ccaccctttc acaattttaa caatcattgt atcattttat gcaaagaaac
    19141 taaaggtata attttctaaa aattctaaca atacaaacaa ccatttcttg ctaattttgt
    19201 actttttctg tctacacgtt gttttttgag atattctaag ttaggatgtt attaatgggc
    19261 aacttatcag gaaacgtttt cttatgtttt tgtccaattt ttttgtgagg tgtttattct
    19321 tgaaaaaaat aaagaaggag agttatggct tcctgacaat ctttgtcgct tgtataatga
    19381 tggttttaca gcgtatattg atttcagcag tcactattcc tacatatgca acaaaccctg
    19441 gtcgattcta catctataca tttttacaaa cttccgttgt tattggtgca aatttgatcc
    19501 cactttacat gggttaccaa tatcaaaaaa tcgcccgttt aaaagtttta cattatttaa
    19561 gtcgttttgc ctttatattc ctaatagcca cactagtttg caatatcttt ttttacgtac
    19621 aagctggcag cttgaatatt cgtgattatt ggcttatcct aacacctatc agtcagaatt
    19681 actttactta tgctgtttct tgtactttgc ttttttgtag tattccttat acagttggat
    19741 ttttaaataa attatcgaaa acaacaatta aatatctact attattttta acaattggat
    19801 tagttggctg ctctactcta tttaataaag acccgtttgc attacagaat ggacagagta
    19861 ttttatggat tttttattta tttttaatgg gttatggttt gaaagaatgg aactggaaag
    19921 aaaaggtccg ctataagctt cctcaagtat ttatcagctt gattgtttta ttcggtttga
    19981 ttattttgat gacacaaatt tcactcatca tcagaggaaa tacagataca gcgctgaggt
    20041 tttctaaacc atattctctc ttcgcaatgt attatacttt ctcttcgttt ttatttttag
    20101 aaatgctatc tgataaatta ggtataaaag gaagaggaaa ttttcttgct gtatttttga
    20161 ttgtcactca agttatatgt aatttccctt tagtttctta tcgcgtatct acatttatga
    20221 aacgtgaatt tccaaactca gggaaggcat ggctgataaa tatcgggcag ttttttattt
    20281 gatatttatt agcagtgtcc ctcctagtta tctttttatt ttatatacaa aaaatccgtt
    20341 tcattcatcg tttcatcgaa tacttcgctt ttgattcgat tgaacaatta actcagcgtt
    20401 ggaaaaaaag aattcattgg ctttttgttc ataaaagtta ttttttagtt gcagcatttt
    20461 tttactgctt taccttcgtg cagattttcg ttttagaacc aaaagaaggt tggaaagaaa
    20521 ctatccaggt tgccttaaaa atatttactc agcgacaagc acccatgatc ctgacaatca
    20581 tcattattat gggatttttc ttcttactat tgttgatttc caatcgtttt tggtacgcct
    20641 tgactgctac cttgatcatt aacttactgc tcactatttc gacagtaatc aaaatggaaa
    20701 tgcgggaaga gcctgtcttt ccttcagatt taaaaatgct gaccggattg tcagaactat
    20761 tatcgatggt tagcccagtc ttattaatcg tgggcggatt gatccttctc ctattattga
    20821 ttactagtat tattgtacaa cgtagactac aaaaacagta ttccttgaag attcattgga
    20881 aacgtcgatt tatcagtatc gctgtattat taggatgttt ttcgggtgtc tttttcatta
    20941 atcacaaaaa ttcgccttct ttcttgctgt ttaatttatt taaagtaaac aagactttct
    21001 ttaaccagca aggcgccgta aaagataacg gaccagtcat tcaattccta aacaatatcg
    21061 acattaaaat catggaagaa cctgctggct attcgaaaga aaaaattaat ggaataatga
    21121 aaaagtacaa cacaaaggca gatgaaatca accaaacaag aagtgactgg ttaaacaatc
    21181 agacgatcgt tctcaattta agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga
    21241 cagtacctac agaccctatt ccttacatca aaagcattat aacagaagga acaggaggat
    21301 tgatgttatc cgtcggttat ggtggcggaa cagctaacat ggaatgggaa ggactgacag
    21361 gcttgagtat cagtaacctg tctgcttcgt tagttacacc ttatacccag ctagtggagc
    21421 gtcagaaaat aagtccaaat attaccgatc tattcgatga aaaaatagcc atccatcctt
    21481 ttacagctgc tctttatcgc agaaaacaag tgtttgataa atttggtttt gacaggttct
    21541 attatttaga cagtccggat aaattaactt atacggataa aatagaacaa agcccacgca
    21601 tatcggatga ttcagcatac aaagaaacct tgaaagcttt acaaagcaat acggaaacgt
    21661 cacaattcat ccaattatcc actatgcaaa accatatgcc ttatgatgat tactatagtg
    21721 aattgaacta cactgctgaa ggaactgctg tcattcctaa tagaagaaaa gaattacaaa
    21781 cttatatgca aggccttcac tataccgata cagcagtaaa aaaattcatc caagaaatcg
    21841 acaaaatcga taaaccgatt acctttgttt tttatggtga ccatttaccc gcaatctata
    21901 gtgggaatga ttcgaaaaaa tatggattag aacagcacca gacagattat ttcatttata
    21961 acaacaagta tagtcgtgaa caagcagcta cgttaaataa agaagttgtt gctcctaata
    22021 actttggtgc tatggctctt gagcatgcga atatcaaagt aactccttac tatgcgttgt
    22081 tgacagaagt atctaatcat ctacctgcat taacaattga tcctacagaa agcttgtcta
    22141 atcgttttaa tggaaaacag gtctttgtta cagaggatag tcaaattatc caagaaaaaa
    22201 atttaactga gaaacaaaaa gaaattctag aagattatcg tctgatacaa tatgatcttg
    22261 ttgcaggtaa ccagtacagt gcaacatggg cgacacaaaa aataaaagac taacaaataa
    22321 actgagcaag gggttgtgat aaaagacatg tcacaacccc ttgtttatct cattaatttg
    22381 cttgtccata aacgatttga ttaaagccat ttttcaattc attattcgct gtctcgttga
    22441 agtaaagata attatcggga ttcccctgat tttgttgtgt caccaatact tttggctgat
    22501 tttgctgaaa cccgaataga tagacgtgct ttttcagata tggttctgtc tctgcatcgg
    22561 aatagacggc tgtgatttga tagtctgctt gacctgtccc actttctgac cattctgcag
    22621 aaacaggcac tccaccaata gccatcttcc actccccgtt caaaatagct tgagggactt
    22681 taagaccata taagtccaca ctcatttgat tcgtataact tttgtattgt tgtcccattg
    22741 tttggcccca tttggtcata aatgcctcta attgtgcgga tttgtctgca ttccacagtg
    22801 tttgttcaac ttgttcgctg ctttctgaag aggaagatga agcctctaca gaagaacttg
    22861 aagtagatgt ttgagttgtt tcagtcgttt tggaagactc tgacgaagta gaggtatctg
    22921 ctgtttctgt acttgctgca gttgacttgc tctctgtgct agttgttgta gtagttgcaa
    22981 ctgttgcttt cgctgtttct tttgttgatt cttttttcga ttcattcccg ttggtacaac
    23041 cagctaacaa aaaagctaga ccaattgtta ctaaaatcct ttttttcaat taaaaaactc
    23101 ctttcgatta ctcccacttc tttattctat caaataagca atgtgatttt attgattttt
    23161 cgtattttct catcaatctt tcaaatcttc taaaaactga gaagcacccg ctgatgttgt
    23221 ctgtaataca tcagcgggtg cttcttttat tttaatcttc gtgattttcc aatttacctc
    23281 acgctttata tttgtatttg aagaaacata gacttttagt catcatcgtc ttcctcatcc
    23341 aaatcttgtc ggacagtttt tccttggagt ctttgtactt ggtagatgat ttctttattg
    23401 aaacttaaaa gttcaacaag caattgttgc aagccatatt tttcttcttt ttcagctaat
    23461 ttgattgccc gtgtgataaa gacattttgg aaatcaaaat cttttgcaaa gttttccaac
    23521 attgttgagg ctggtaaata tttgttttga ccatgttcgt aaatttttcc ataacgcatg
    23581 acttcttcta aagtagacgg aacgatttca ttttcaatca ctaactcatg agagatttta
    23641 ttgaaccatc tcgcttcttg atcagcatat tctgaaaaaa gggtacttaa ttcttgcgaa
    23701 gatgtaccca aaacatacaa ttgtgcttgg atcaatttca tttcttctaa tttaaaattt
    23761 gctaatacat gccccaccat ggcgccagca gttggtttat gatggtcgat atctgcttgt
    23821 tttgtttctt gaacaaattt ttcttccact atattcattg atttacgcct cttttacttt
    23881 tgctgattgg acttcataac ctaatttttc cacaactgca gctaattgtt cagggtggac
    23941 ttcatctgga tcaaagtttg ctttgatttt gctcgcatta aacaaaactt tcgtattgct
    24001 taccccaggc tgttgagaaa ccgcatgttc gattttttgc atacagcttg gacaagctag
    24061 tgtatctaat tgcataataa tttttttcat cgtagttctt ccttcctttt tcttgatagt
    24121 cttattctag attacagctt ccataaaaaa attgattctt atcaagtttt acgatttttc
    24181 ttctttgctt tcgtgtccaa aatacaataa acgcatcgcg ttaaagataa cgatcaagat
    24241 actagcttcg tgaacgaaca tcccactagc catgtaaata aacccggcaa ataagccgtt
    24301 gagtaaaaat aagacgacac caatggcaat cactacattt tcacgggtat ttaaaacagt
    24361 tttcttagcc aagcggtagg catgaaccaa cgattcaaaa ttagattgca tcaagacaac
    24421 gtcactcgtt tcgatagcaa catccgttcc tgatcccata gcaatcccga tatcagccgc
    24481 agccaaagag ggagaatcat tgattccatc tccaataaaa gcaacgcgca atccttgttc
    24541 ttggtatttt ttcacaaatt gaaccttttc gtctggtaat aattcagcat ggacttcatc
    24601 aatgcctaac atatcagcta cataatcagc agtcatctga ttatcacctg tcagcatcac
    24661 taactgtttt gctcctgctt gtttcaactt agctaattgt tcagcgactt ctggtcgaat
    24721 cacatctgac acacctatga tttgagtgat ttttttgtcg attgctacaa taatgaccga
    24781 gctgcctgtt tgttgtaatt gatgtaaatc ttgtacttgc tttggtgtga gagaaagttt
    24841 gtgagcggca atcaatttgg tgttgcccgc acaaactgta tgaccatcaa tctcagctgt
    24901 gattccttgt cccttgattg tctgattatc tttgactgct aattgttgaa aatcaatgtc
    24961 tttctcctga gcataatgga tcacggcgcg ccccagagga tgatcggata acgtttctaa
    25021 tcgagccact aaagctaagc tgtccccttc atcctctgta tagttcttga tagtggatac
    25081 agcagtagtt cctttggtca atgtaccagt tttatcaaag acaaaagtat cgattttgga
    25141 aaaagtatcc attgcttcac cgcctttgac taagatgccc cgtttcgctc cattaccgat
    25201 tcctgctaca ttcgaaacag gcgcaccaat gactaaagcg ccaggacaac caagaaccaa
    25261 aacagtgatt gctaaacgaa aatcacgact aatcagacca acaaccaacg caataagtaa
    25321 aacggttgga gtataatact gagaaaatcg atcaatgaat ttttccgcat gggatttggt
    25381 atcttgtgct tcttctacta attcgataat tttggcaaag gtcgtatcat ctccgacctt
    25441 agttgcttca atcgtcacgt agccatctgc taaaatcgtt ccagagaaga cttcatctcc
    25501 actgattttg acgacttgtc gtgattcacc agtcactgcg gcttcatccg catacccttt
    25561 cccgtcaatg actcgtccgt ccacaggaat agctccacca gtttttacta agacgatatc
    25621 gccttcttcc acgtcatcga tttccacttc ttcagtcgtt ccatcttctt ggacaacact
    25681 agcagtagat ggggccatct cggtcaaagc cttgattgac tcccgcgttt tggtcaatgt
    25741 tttttgttcc agatagctac caaacaaaaa caagaatgta acgatagctg actccttgta
    25801 ttctccgatc ataaaggcac caagcacagc aatagtaacg agtaaatcaa ttgagataac
    25861 tttgactttc actgcctgat acgcatggat cataataggt atgaccgcaa taatagaagc
    25921 tatggctaga gcaatgttat agaacaagac aaaaccagta aattttccga taaaaccaaa
    25981 aataatcaac acagctgata aggcggtgat atgatttcga tgctcttgga tccacttttg
    26041 taatttcatc tttctccctc ttttcctttc attcttacga gactaagtat agatacttat
    26101 ccatcataaa aaattgacca ctatcaagtt tagtcaaaaa ggcggttttc ttttggtaga
    26161 atacactaca aaaaatagtt agcaagaaga ctcactaaaa gtgatcttgc caactattgt
    26221 cgtttctatc gaaattttca ttgaatgatt ttcctgtaca gcatacatcg gagatctatt
    26281 tctttatcta agatttttat cttgctaatt ttttccttta gtctttcaaa tccacgtcgt
    26341 tcatagaatt gatacgtaca gccactatct gtaaaaagga atatttcttt tcctttctct
    26401 cttttttcaa attctgttag caacttggta ccgatgcctt ttgttttcaa atcaggattt
    26461 gcagctaaaa aaatgatctc tccatctggc gaatgcttct ttaaatatgc tgcaagcatt
    26521 tgttcatttg ctgtctcgta agctccttta ctttctttag caaatatatt ttgtaaaaaa
    26581 tcgaatatct taacatatat ttgctgtcca aaagaacgat gtttcttttt ttctcctttt
    26641 atctccgcca gcaaaacacc tgccaactcg tcctctgcat acaaagcaag aatttgagtt
    26701 gctcgattca actccatata caaaaaatat cttccgtaaa gattcagcag aagttcattt
    26761 tccatatatc tctcaaagtt cattccttca acagcaaaat agatagcttt tttgtaatct
    26821 ttcttttcta agggtttaaa agttagtttc atcatttcgc ctcctcaact tcaatgattg
    26881 tgttatactt acattataat agacacagtg tctactatct ataatcaatc atctataagt
    26941 ttgtcgatta agcgacagct ttgctttgat atgtcggata aggagatagg taaatgaaaa
    27001 agacagatag acgcgtcaaa aaaacagaaa aagctttggc tgaaacacta tccacattat
    27061 tagtaaataa aaagattcaa gcaatcacta tacgcgaact aacagaaacg gcagatgtac
    27121 atcgttcgac tttttataca cattataagg atatctatga tctatatgat caattagaaa
    27181 gcaacttttt tcgggatcta aatgaaattc tctcttatga tccagcccat agctatgagg
    27241 aactatacac tcgcttgatt gactatgttt acgcaaatac ttttttatat cgcttattta
    27301 tcctcaacca tggtgatatt tcgtttcaga agaaagttaa acaagttatt gaagaaaact
    27361 atctgaacat atggctatat gaagacaaga aaacgattat cacagaagaa atgagattct
    27421 ttactacgta ccacgtccaa ggttgtctat ccgttctcaa tcggtgggta gaaagcaatt
    27481 acaagtactc caaaaaagaa ttattagaac tcttacagaa actgaatgat cacatagaga
    27541 aaatcatgcc ttgaataaaa acatactaag cttagttaag aagaaccgta accttctcgt
    27601 ataacaactg aatcttattt agccaaattg tttactgaag attttttgag taaaatttct
    27661 ataattccta cttattgaaa agtagtttgt agtgctgctt ctccctctct aagtgatata
    27721 tcttgttgta ccaatcaaat tcacctcatc attaataaat tacttttagg ctaatttatt
    27781 acaacttata ggttgaagct gtaaccaatt tatagtcata ttatattttt atcatccagt
    27841 ttattgaatt cttctcgttt acctcaattc attttcaagg ggaatcagct catcatctaa
    27901 cattttacgc catgctcgct tcatttcttg tgggtattta aatggtttcg cttctggatg
    27961 tttggcttta tatccttcgt aatccttact gttgataatt gctttcatat taggaattgg
    28021 atcttcacct ggcatatatt gcagattaga agcatgtaat tttttctcag aaacaaacca
    28081 ctcattggaa aagacatgaa tcaaatcatc acttacttgt gtaaaatagc gacgtttaat
    28141 gacaaagata tcttcattgc tatctagtgt accttcattt ctttgtgctt aaattcaaag
    28201 ataattttct caataaagat ttttgattcc agtaagattc ctttactcct tttacaacat
    28261 agcgttgtcc tttgttttga aacatcttct tcccctcttt gccattaaaa atagcccaat
    28321 tctctataaa gaagattaag cttcattatt ataatataaa gtttaaattt ggtttattac
    28381 ttactttttc ttattctcta gtaagctaat attgaaaaaa atgtaatcag atcattattt
    28441 ttatatgttg taacttcctg ataagaaaac taattaaaag aaagaggtaa ctacataaac
    28501 taattaaaaa agctaacttc atagttggag gataaatgga agaaacttca atgattgttt
    28561 ctccttgtgt tccttcaatc gacactagtc ctattatcaa tctctctttt ttgtccattt
    28621 acttttgcga ctgttcccca ataatcaaat acaggaagcg ttattttttg tcctttagat
    28681 tcatgtactg tatagactat tttcgaatcc atcacttgat aaggcatagt atgccattcc
    28741 ttgccaattc ttacttcatg attctgcaaa tgttctttgt attgtaatgc ttccgtgggt
    28801 gcatagtcat aaagtcctcc gggaaggagt gtcgtgtaac gaataagatt ttcttgattc
    28861 attaccaagt gatctctctg agtgacaact ttatgttcat agtttgcttt tgttgcagca
    28921 gatacagcca acagtaataa aacaattgcc gttatattaa ctacttttct ttgcttagct
    28981 gatgtgctct tcatgaaaac gaacgataaa atcaacgagc caaacagtac ttgaaagcct
    29041 aagattcgcc aaggaaattg aatcatttga atgggtgtat tttgtaataa agaccatgga
    29101 aaaaatttgg tggataagag ccaagagact accgcactaa ggtaaatcac tttttctttg
    29161 cgattaaatc ctttaaaaaa gaagagtgga cagatcaaag aaataatacc aatgattcct
    29221 accgtaaagg ttgtcaggtc attattcatc gcaccaatca aactatccga gacatttaat
    29281 gcttggtgtt gtaaggtcgt tttatctggc tcattaatcg attgaaccat gatttgtcgg
    29341 aacatcggat accaaaagta tgctgttaaa agaatcgtca ttcctactgc ctttgacaac
    29401 tccacaatca accggatgct ttcttttttt cttgtttcac ttactacaaa gccgcctaag
    29461 actagtaagc caacggctat tacactcata aaagtggaaa gaacatgtgt ataaatgagt
    29521 aagctcatac tgactgctaa tggaattgca catggctttt tctcataaat cagtgcgtaa
    29581 gcataataca taactatcgg tataaccgta agtgcaatcg cttcagcaat agcagaacga
    29641 tgataaatat caatcgtacg gtacccataa aatgtatata aacagctaaa tataaaagcc
    29701 gcatcgattc tttttaaaaa ttttagtcca taatgaaaca tcagcatgat agtacaaaca
    29761 ttcaatagcc aaacatataa gatataacta acgattaaat tattacttat ccaataaaaa
    29821 atgactgccg gaaaaaaggt caagtaggga taaaaataat tcactccgct cccataatga
    29881 ttaaacgtcg taaaattgat tggaccagaa aaaattgaag ataacccttt gactctagca
    29941 aaatggaagg caatatcccc cgatgtagaa atagtatgaa catttctaaa ataattgatt
    30001 gaataaagtc cagcaataat tgctaggaga atggacaata ttattttttt ctttacttgc
    30061 attacttaat tatatactcc tttcatggat tgctttatct agtataactt aaattttaaa
    30121 taaatcgtta tttattttta ctctcactta aaaaagcact aaaattataa gagagggagg
    30181 taatggtctg aacaccaaga attgttccaa catcgaattg cttaactaat gagttcaaag
    30241 cttaattatg tggctcttat atttaccgag cgtggattgc gatctcttta gcgaaacaaa
    30301 catctttcat aaaaaaaaca gccatatccg ctttcttgga tatgactatt tactactact
    30361 ttactatctt tcattcttct tgtctcactt atgataaggc gctgcgtatc atgagtgaat
    30421 gatattagga aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt
    30481 catgttagaa gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa
    30541 atcaattaaa ttccataaaa tataaataat agacctagca tcatctggta taaaatggat
    30601 gctaggtccg ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta
    30661 ttttagtagt catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata
    30721 aatttgtacc atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc
    30781 aatataatgg cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc
    30841 taattcgaat caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac
    30901 tttttcccat agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat
    30961 caactagttt agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct
    31021 cttgtatatc tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat
    31081 agccatattg aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag
    31141 atgatttttc ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt
    31201 tttagttgca gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag
    31261 taataaaaca ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc
    31321 aaacaatact gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt
    31381 gattcgtttt aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata
    31441 aggaccataa gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt
    31501 atcagatgtt tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc
    31561 tgttttcatt tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag
    31621 tgtgaagttg ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat
    31681 ctctgagaga tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc
    31741 tgctaagata tcgatcaaac gtaaagcggc tactcgtcca gtagcagatc ctgtgattgt
    31801 caaccataaa agttcccgtt ttgtatcata tactaagccc cctacatgtg gccgtttagg
    31861 taaaatcagc agtttgatcg gctgtcctgt ttctctatcc agtacatgaa taatagaatg
    31921 atgctgatgg tcatagcagt aggcagaaat aaacacaaaa tcttgagaaa aagccaaccc
    31981 ttgcggcgtc atccattgac aaacctcatt ttcgccggtt tccggcacca ccattcttgt
    32041 ttctgttaat ccaggaatgt tatattgttt tgcttcagcc ttttcccaag ctgcttgaac
    32101 aataggatac cgttttaact ttttttcaat atcctgatgt atccgttgct gcttccaatg
    32161 cttaaacttt aggacatagt aaacaagaag gccaatgacc agcaacacta aaatcattat
    32221 tgctacccgc tgtgtcattt ttttgcctcc ttgttctatt cgatcaatcg tccatatacc
    32281 gctattaatt accgtaacta catgtttctt ttactagcgt ttctttatag gatctttcat
    32341 aatctcattt atagtaacat taagaatccc ttattctact atagtcactt ccacatcaat
    32401 attgcctctt gttgctttag agtatgggca aaatgcgtgg gcttcttctg cgattttctc
    32461 tgcttggtcc ttctcaagtc ctggcaaaga aacttctaaa acgaccccga tttggtagcc
    32521 cccatcttct aattgaaata aagatacccg tgctttgact tcagaatcag actcaagatt
    32581 attttcttcc agcatgtgtt gaaccgcgcc gttgaagcaa gatgcgtatc cagccgcaaa
    32641 aagttgttcc ggatttgtcc cttcactatg gattcctggg cggtcgatct tcatgtgcat
    32701 acttccgttt ggtgcctcaa cttcgccatc acggcctcca cgattgatca ttgctgtttc
    32761 gtacattttt ttcattgaga tcatcctttc tttcgtcgtt ctatattaag tgtaacgagt
    32821 attttttaat ctgacaaagg taatgcttgc attcggcgac atcagttaga caacatcgat
    32881 gatcagtgat agaagcagtt tatctataca aaaaaagaag ggttctatga tatttggtgt
    32941 ccttactcgc cttgagtaac aacaccaaat atcatagaac cacttaatca gcttcacact
    33001 gctttttcta ctttcgtagt tgaaaaaaac ttaaacaaat catcggcaaa tagttaaaga
    33061 aaaaacttaa tcgttgaaag aagcccaatt gcttaaagaa ggcaaactga ctaatcgacg
    33121 gaatccgtgc taatccgtaa agacctgcaa ttagtaaact aataactgtt aaaacaagat
    33181 aaaaatggct aagcgtacct tgaccacttt gacgataaag cagaacaagt aacaatggga
    33241 aaagtagaaa gcctgcatag cctagcccag aacctatatt atggatccaa gtcgatagat
    33301 tacaagagct ttcattcgta ttgatactga ataagccagt aaaaatacaa tcacctatcc
    33361 catataaacc aactgttgca acaacaagta ttgcaagtgt ttttgatgtt ccaacaaata
    33421 ggtgatacaa cgcaggcaac gaaagaacaa aaaaaagtcc agatacaact gaccagacca
    33481 agaaagcacg tctgactgga ctatctacgt caccaaacac actaattacg gtagtcattt
    33541 gattcagttt gggataaaaa aggccaagga tatacggtgt cgataaatca ctcatcacac
    33601 ctaataataa aaaataaaaa ccatattttt gcaaaaaaat cattttcttc ccctactttt
    33661 tagtgattat tcgatacatc caacaatcaa attttttctc attttaacac atatttttat
    33721 tctagttgtc ttttgaacaa tcaataacat aaaggtgagt ttcttctaca ttttaaaaaa
    33781 tcgaatggca ctgaagagtt cagttgtcag catcaatctc ctactcaaaa aggcaaaaaa
    33841 acgtaagtca gatgttgttg acatctcact tacgcatcca ttgattgaaa atttcttagt
    33901 aaaatctact agcctaaaaa tttgaagact tcttctcgat catacagttt aaatactttc
    33961 tcgcataaaa attgagtaat tagtgccgca aaaacaggga tgactaacca acaaagaaca
    34021 acaagaaagc catttagccc tgcatctaaa gaagccaacg gaccaacaag tccgaccacg
    34081 ccgaaaccag cagaagcagg cgtacctgaa atagaaaata gagcgacagg aatagctgta
    34141 atcactgcac taactaaaca aggaataaga atcacaggtt ttctaaatag attaggcatc
    34201 atcatcttca tagcgcctaa agcaatcgca attgtcacac ctgacttatt aaccttccaa
    34261 gagttcacta ctaaaacaaa agtggttgca gctacgccca tcgcagccgc accagcagaa
    34321 acaccatcta gttggattgc cattccgatt gctactgttg aaataggaga gataatcaaa
    34381 aaggcaaagg aacaaccaat caagatagac attaacagcg gttgtaaggt agtgaagtta
    34441 ttaatcaatg atccaattgc agaagtaatt gctgttacaa aagggaataa ataaaaacca
    34501 agtaaacctg cacctacacc tacaacaatc ggtgttaaca caatcgacac tgacccaaaa
    34561 ctatttccaa tcagtaaaat cagcccgaca gcaatcgatg ctgtcagcat cgtattaata
    34621 atatcgcctg taccagcacc aacgtaaaca cccgtttcac ccaaatcagg tgtgaatttg
    34681 actacaccag aaccaacaaa agaagctccg gctacaacca tcattctcat cggatcaaat
    34741 ccaaattgta aggcaatcaa accaccaatg atcaaaggtg ttgctagttg aaaaataact
    34801 gctaactgtg cgatcgttac cgcaaaggga tacgcactaa aatattttaa aatggcacct
    34861 aatactgcat taggaatcaa tccaataatg attcctgttg ctgtaccagc gaggacttta
    34921 ttcagaaaaa ttttcggtga tattttttgt gaagtttcca ttcaatctct cctttatttt
    34981 acgttcaaac tatcttcttt tgcatggaca agctcagtca agaaatcaca ataaggcgtc
    35041 gcaacaccat atttcttccc ttttctagaa attgcgccat tgatataatc gatttctgtt
    35101 aatcgatggt tcttaatcaa gtcttgatac attgaaggat aatgtaaccc aattgtttcc
    35161 gggtcaaaac aagattcaca atgtgcaatg acttctggga catctagttc aatcttctct
    35221 actgctgcta ctttggcaaa ctcattgaca atcgtcgcca ccattttatg agcagtcgat
    35281 gtttttccta gctcagccat attaacgtct aagattgtgc atagtccgtt cattgttccg
    35341 ttcacacatg ctttgcgata aatagaatag tgaatattgt cagaaaaatg agcatttaat
    35401 cctgattcag acaatttgtc tgctaatttt tttgcagctg cttcttttcc atcacctaaa
    35461 ttttgtaatt ctactgaacc acttccaaat aatttgacct gaccaggacc ttcaagacct
    35521 gccgtccaca tggtatttcc aatatagata ttttccattg gtacaaattt ctcaataata
    35581 tcttcatgcc cgataccatt taatagacat aagacctctg tatctttttt gattaatgat
    35641 tggatatcct gcagcatttt ttccagctgc atcgctttgg taaatagaat aatcagatca
    35701 acttgatctt ctttttctac ttcggattga agaacgattg gtagttttgc ttctacctct
    35761 ttaccattaa aatttgcttg taatccatgt tctttgattt gttggacatg ttctgcccag
    35821 ccatcgatta ataatacttc gttcccactt tggtgaagca tcaacccaaa gcgacttccc
    35881 attgcgccag cgcctgcaat tgctattttc attgattctt cctccttttt ctttctatta
    35941 tactcatttg agaatctttt cacaacctcg ttttgttatt cttctcttat ttatcatgtt
    36001 tatatccacc attcaagtta ttttatcagc aaatcaataa aaactggaat agaatcccaa
    36061 tttcatcgta agagaaacta gactagcaat aagaaaatag aaacattcat agaaaaaatc
    36121 tcactcctct gattataaaa gagggggcat taacctcgtg tatactaaaa ccttccattc
    36181 attcgatgga tgattttctt tttgtgaaaa aatattcatt gtagtacgat gttgcgcaac
    36241 acagacggat ggtattgctt attatttatt aaaatcgaac aatacactta tttaaaaact
    36301 tctttcccag attttcttcc atttttacag ttataactag tattatcatg aaaaatagcc
    36361 ttttattgtc tatgggaata ataatttaaa attggtactt ttttattaat tagttcatcg
    36421 cccaataatc tctccagttt cttagcatct cctgactcaa ccggaattat ccaagcccag
    36481 tctaaataat tttttaaaat ctcatcatat tttgcaaata tttcattgcc atattccgtc
    36541 cttaaaatta tattttcagt gccagacaag tgatttatat tgagttgata aaaaagatct
    36601 ccatctaaaa aaatatcatt agaagaagat ttccccacag taatgaccat tccatcagct
    36661 ctatgtgtta catacaaata attgtatgac ctcatttcac ctaatgaatc aattatttct
    36721 tgccctttag ctctaatata ttgatctcta ttacctatct cactcttatt taacaaaagt
    36781 tttttcatgt caaatttaat cggctgtttt atttttgtaa attcgcttct actactaatc
    36841 caatccgaga ttaatttttc aacatgtccc agagtaaact ctgatttatt cgctaaatac
    36901 tttttttgtt gtattaaaga catctttccc cgaagctcaa aattgagcct acatcgattc
    36961 tctatctctt caaaaagcat cttatcttca tcattcttat attcatcatt cataaaacca
    37021 atccagctcc tcgattaaga taatatttta atctcataca aaaactagct ctcatcaaaa
    37081 caaccattgt tttgatttct catgaaattt ctttatactt ttctccgatt ataaattcct
    37141 aaccccttga gaaaattacg catatattta tctccgcaag gcttaaaatt tcgttggcct
    37201 tcttttctca aaaatgcccc gatttcgcct tttgatggat agatttctgc ctcagcaaaa
    37261 acgtctaaaa tatcatcagt ggtcatagac aaggcgattt ttaatttttt tagcaacaca
    37321 ttgttgatat atttgatttc cttgcttttt tgcggcgtgt ctactatcgg cttatctttt
    37381 tgcggaccac gcgccaagag aatcaggcca tttaggaatg cgtcaaaaac ttggttgttg
    37441 atggtttttt catacacatt attttctgca ttatctttgt tcttatcctg tactttggtc
    37501 agtaccgcct gagcttcttc tttagtcagt ggtacaccgc ctagctcaaa agctttaatc
    37561 aaatccgtat ctttcaaatc aatcgcataa cgcaatcgca ataacctatc attgttattc
    37621 atttgttcct ccgaagtatt tagtctttac tttcttacgt actttttgtc ttgctaagat
    37681 ttttaggatc aatgataaaa accgcatcac ttatgataag gcgctgggta tcatgagtga
    37741 atgatattgc tgaatacaaa tagtccgacc acattaagtt gatcggactt cataagcatt
    37801 ttattgacta atactttttt gcttaagaat tgattctaac atcttttgct aaagttaagc
    37861 atcctatagt tcctgcatta tctgctaact taggtgtgac aatgtattct tcgatatttg
    37921 gcgtatcgac atagccgtta attagttcta aaaaacgtct ttgtactttc tttttcagat
    37981 gttcttgttg catgacacca ccaccaaaaa ttataatatc tggtgaaagc atcagggtcg
    38041 tattacaggc acattgtgca atataatccg cctcgatttc ccagaacgga tcttctatag
    38101 aaagatcttg tcccttggtt cccgtacgca tctcgatagc gggtccagat gccaaccctt
    38161 cgagacaatc atgatgaaaa ggacaagtac cagagaaagt atctcctgaa tgtctcttaa
    38221 ctaacgtatg ccccatttct ggatggctaa acccttcaac aaaaatccca tcttgtatag
    38281 ctcctccacc aattccagtt ccaacagtgt aataaacaac actggataaa ccttttccat
    38341 gacctgaaac atactccccg taacaagcag cattcacatc ggttgtccag gcaattggaa
    38401 tttcgaaatg cttattcaac gttccaacaa aatcaaaatt tttccaggca gtttttggag
    38461 tagaggtgat atgtccatag gtctttgatt cacgatggat atcaatcggt ccaaatgacc
    38521 ctactccaat gctttctaat tgttctttat agtgattaaa gaaatcaatt accaaactca
    38581 ttgtttcatt cggtgtagtt gtaggaaaac tcactctttc taaaaccttt aaatcatcat
    38641 tgccaatggc acaaacaaat ttagtcccac ctgcttctat tgagccatac atttcttact
    38701 ccaccgtcct tttatctagg aattcgttct tccctgtcta atagtttttc gaaaaactca
    38761 tgtggttcct tttggtacca ataggcaaca gaagaaatat cgtcttgacg ctcataaagc
    38821 ttgatgtcat catttcctag ggtttggaaa gtaacacgta aattttcttt gaaactaatg
    38881 gggtcgggta aatgccagcg gtacaaccca tgcatcggta aagcatcatc gccaaaacca
    38941 tgaactggat ttggattagg ttttccagca ctaaaataat cccgggtgtg atctctttta
    39001 gattgaaaag gatagcccat aaatagcgta ttaaaatttt gagcatatgc cctttctcca
    39061 aagttctttt tatggaaagc ccaagcgccg ccaaaataat cttctgctcc tgtagatgtt
    39121 attgtaggga aatcttcatc tccatctata taaaacttaa attctccttc cccccaccag
    39181 tatctttcca atgcgcacaa agcaaagtag gttcctacat aatacccagt gcctttgatt
    39241 ccatcaataa gggtgtaatc ttttcctttt gtcgtgatgg gttcacggtt ccatttagca
    39301 tgaaaataca tcgcatcatc cggtacttca gtcaatgcat aattaaatgt ataaaaaatg
    39361 tgcttaataa actcaggatg ttcattcgtt aaagtaatct tagctttttt tcgaaatggc
    39421 atctcaaaat agcaattcat ccctgatgtt ggattcacta cgattggtaa ggaattcact
    39481 tcgcatcttg tcccaaaccc attacaaaaa aaatctccta atggactttc tacagaagga
    39541 gtggattcat catcccaata aatacgcaaa attaaatcac gtaagacgaa actcccagcg
    39601 attgttttgt ctgtcactgt catccaaaaa tgacgtaaaa ttccagggcc ttccatttct
    39661 gctaaagtaa ttgtctctcc agcatttatc ggaacagaag gtgacccttt tctcccaatt
    39721 cctagatgac tcgctgtcat tgccgctttc cctttttcac catttttatt ttctggtgaa
    39781 atagttctac tttcctctcc cttgtggagc catatattat ttaaacaatt cattttattc
    39841 gtcctctatc ctttcttcac ttttttgatt attcatatag atgatttgtt gttgtgcctc
    39901 cgcaataatt gctccaatat ctaaattatc atttcctaac tgcaaagcga ttgacattgc
    39961 caagggaaga ttcattcctg caattacgtg cactctgtca gaaatatagg taaaaaactt
    40021 ttgatttaca cttcctccag ccatgtcagt taaaacaatt atttcgtctg tagtatccac
    40081 taactcttta atttgttcat ctatatcatc aatttcttcg acatatgcac ataagctact
    40141 aatttgatta tgcatacctg ttaaaaactc tagtgtctct ttaaaaccct cagctagtat
    40201 tgaatggcta gcaatgatca atcttctcat aattcaaatc cttttccttt acgctaatat
    40261 tccaaatgca gccataacca tcgcaaaaac aataacgagt agaatcaaaa tggtcatact
    40321 gatttttttc ttactgagta gataatatgc tgcagcagta ataattactg gaattaatgt
    40381 tggtaaaatt ttgtccaaca ttggttggat tttcatacta acttcaccaa aagtgaattt
    40441 aagtggcgtt gaaacactga taactgaagc aattaatgat ccaactactg ttagccccaa
    40501 tactgaagca gcttcagtta aataattaat cttttcaccg tattcagtaa caatcttcat
    40561 tccctgctta taaccaaatt caaagcctct cattcggaaa aagaagatta ccatgattaa
    40621 aatgaaccat aaaaagattc ctacaggatt attttctaaa gccatataag ccgcaattga
    40681 tcccatgatg gttgggatca tcgtcatcaa aagagagtcc ccaataccag caattggacc
    40741 catcgtacca actttaaagt cattaatcgc atcttttgct tctaatcctt gcttttcctc
    40801 cattgcaaca caagcaccta aaataatggc tgccagccaa ggctgtgtat tgtaatactg
    40861 aaagtgatta tttaaagctt cgatgtattc atcatcatct ttgtataatt ttcttaaaat
    40921 aggtgacaat gcgtaagtca cagatgcacc ttgctgtgtt tgataattaa aggtacaaac
    40981 tgacatcaac caacgatagt tcgctttcct aagatccttt ttcgttactt gatatttttc
    41041 gctcatttta ttcatcctcc ccaataaacc caacatcaga agctcccgca gaagcagtga
    41101 tttgtgcatc tttttcagct ctatgataca cattaaatgc taaagcgaat cctatgatcg
    41161 ccgctccaat aataggtacc ttcaaaaaag cggctaagaa aaatccaaca agtaaataac
    41221 taatgtatcg acctgtaggc ataaagcgta acaacatcgc aataccaaca gcaggcaaca
    41281 tttttccggc taatgttagc ccatccataa accaattagg taaaacatcc aaaacgtaat
    41341 taacagcaga ctgtcctaaa gtaacggcaa taaatactgg aattgctgca gataaaccaa
    41401 agaaaattgg ccccaaccac aaaacactat tcattttctt aaattctcgt ttattcgcat
    41461 attcctgtga tttgcgaaca acaaaaccat ttaagatttt gacaattaca tctagttgca
    41521 cccctaacat acctacaggt agtcctattg ctaatccggc ttctactcca agctggctac
    41581 caaatgccgt tgcaataatt gcgcacgttc catagtccgg tacagatgag ccaccaaaac
    41641 cggcaactcc taatgacatc aattgaactg taccgccaac tatcaaacct gtttttatat
    41701 cacccatcaa aattcctgtg aataatcccc aaaaaactgc gtaatttaca aagatcatcg
    41761 gaatcccata ataatcaaca tgtttaataa atgctaatat agtcagcata ataatttgaa
    41821 aaatagtaaa atgcattatc cttactcctt tatcctaatt ttgattgaat ttcctgtgcc
    41881 ttatccgccg gaacaaattg tgtaacaatt tctacaccag aatccaacaa ttttttatac
    41941 gtgctgatat tttcatctga tgcataggct ctagaagata gctgtaatgc attatcttta
    42001 acaacagtac cgccaacaat tagtcggtca attctaatac cgttatctac taaatgaagg
    42061 attgtttccg gctctttcac gatgacaaat actttttcct gatcgtattt ccccttttta
    42121 aaattcgcaa tcgctgtttc ttcagtgata attgatacag ccattccagc aggtctagct
    42181 aaacgcatac ttgatttttg gatgtcgtta tttgcgacag aatcatcgat caccatcact
    42241 ctgtttgctt tagacattgg tgcccattgc gtgacaataa tcccatgcaa taaacggtta
    42301 tcaactctcg acataacaat actcataaat acttcctcct ttatctttca ttttcttaca
    42361 agcaattacc atgccaaaac aaaaagaact tgataaatca agctcttttt acataatcat
    42421 aaacgtattt aacctcatga atcgggatgt cgacactata ataatgttca acgacactga
    42481 aagattgctt aaaatgtgtc tgaaattttt gaaattcctc actcgtttca ttgaaataaa
    42541 tttcatcttc caaagtattt tgttttacta atcgttcaat taagcaacta atgtgaacat
    42601 ataatccgaa acacgtatta ttatcaaatg aaattcctag atcattttgt aagatgtata
    42661 tagcatctga aacccgttct aataatttag tcggatttaa tatggtcaat tgctccatta
    42721 aattcgttag actaaaattc ttcagaatat tattgttcaa aactttcagt tcattatcat
    42781 taaagtgtct attcaattga ttggagaatt tacttatatt cattcctaaa atcatatctt
    42841 caattgaaaa gaattctaca tcttctagat gtggatttag tgttccaaca ataaaaagaa
    42901 tattgttctc tttacgaagc tccgataaat agttttcatt cactaaagag taatagtcac
    42961 acgtttctat gacaagatca gtatcccttg gaaagctatc ttcaagtatc ttttttaatt
    43021 taaacgaagt tcctaatccg cttgcacaag aacagacaat catatccttc ttaatatttg
    43081 atgattcata aatataatcc acattttgat tttcttttac ttgttcaaga atctgtttaa
    43141 gcggtaaatc taataatatc tgatttgcaa catcaatagc cattcttgtt gaaacattgt
    43201 ttagtagtcc gaattttttt tgagcgtcta gttccaaatt tagataaatt ttctccagtg
    43261 aacccatatc aacaagtaaa ataatttctt catatccggt acactcttgt aagtatctat
    43321 taattttgtc tgcaatctca ctacttgaag tcattagtgg catatcaatg gcgtcgtata
    43381 tatatctctc aacgatccta ttagcagtac ttgctataca ggttgccgtt agatctccat
    43441 gagccaaaat cagtgctaaa cgtttttgct gaaattcttt actacacatt aacttaaata
    43501 aaataaccaa aacatttttt tgaatttttg ttaaataaat atcccctatt tgaataaaac
    43561 gattgactaa ttgaacttct cgactacacc aactattaat atcattgatg aaggaatata
    43621 atttatcgtc cgatgttaat tggatttgat aaatccaaga attcaaaaag atagacgtta
    43681 tttgaagata ataatagtca aattctattt gaaaatctct ctctatttcc ttaacatact
    43741 gtctaaatcg aatctcttga taggtgggat atgttaaagc tgaattattt tggatatcaa
    43801 cattcttttg tctaaaatat ctatcatatt tctcaaaaag ctgctgctta tcagtgtctt
    43861 tcttccacgt ttgataaaga tactctataa gttcgttgat attaaaaact tcgtctactt
    43921 tttccaaatc atccatttga atatatttta attcattctg tcttttcata accttcatgc
    43981 cgttgaaatt cacccattgt ggtaaatgat acatctgaat ataaagttct tccaaatcat
    44041 ctaagaaata aaaggcattg gcacaggtca attgaatgat atttttcaaa ttgccaacat
    44101 tttcactaaa gcgatagttt aataaaaaat ctaataattg ttgagatatc agtattttct
    44161 ttgctagtct ttcttcttct gatttaaaaa atatcttgat taactgtttt ctttcataaa
    44221 gagaacgact gtctaatgtt ggaagtgtta gctttacagg tattcttctc aacaaagttg
    44281 ataatagttt atcctcaggt ggttctgatg ttgcaaatat aatccagcaa cttgatgtgt
    44341 accagttttc attatcaccc atccgatgaa atttcctcgt atccataaac tgaaaaagtt
    44401 tctcctgaca ttcattgctc aatgcatgaa tttcatctaa aaataagact ccattattag
    44461 ctaattctgc taaacccttt cttccatctt cggctcctgt atatgcacct tttacatgcc
    44521 caaaaaaatt ggacaagaaa agttctggat tattggcata ttcactacaa ttaactgaaa
    44581 tgaaagatgc cttctcttct atataattat tggccaacaa atccttatac acataatttg
    44641 ctaaatagct ttttccagtt ccagtctcgc catgaagtaa aatgggtaag ccatttggcg
    44701 gatagtagat tgctgcttta atttgagcaa ttacatttcg cagacttcct tgacttccga
    44761 taaccctttc tattccaacc ctaaaagagc tatcatacca tttttccgct tcctctgaag
    44821 agctaaaaat ttgttgattt ggaactttct ccggtaaaga aaagaagatt actggacgag
    44881 tattgatttt tccaaattct ccatctttta cgaattcatt caaatattga gagacaatat
    44941 tacgactaac atttaatttt tcagatattt tctgtgctgt attgttcccc catttatttt
    45001 taatctcaga gttctctatg acataattga caatgcgttc ttttaattct tccatcacag
    45061 cacctcctcg acactattcg aattttgccg acagtatttt tccataaata tgacttgcta
    45121 aaaaggcttt gtttagatca ttcaattgga acgttgcttg ttttgcttga tccgctaaga
    45181 cgttgaaaag atactctggc gaaatcgcat accccaaaat atcaacgatt gttgcaatca
    45241 gatcttcttt tgattcttca tctgaaagta attcacgata caacatcgtt tgtttgctta
    45301 ctgtgtcata ttcctctaag gattcatctg ctaaaagaac cacttgttcc aacaaacggt
    45361 cggataaata tttatagaaa atcagcccca ataaataatt tttatattct gacgcatcca
    45421 ttttactacg gagattatct gccgcactaa ataacttctg atttaattct gcacccaaaa
    45481 agatacactc ctatctctta tttaaagttg ctatataccc tcttatttta gcatactcag
    45541 ttttagaaaa agcaaaaaaa taaagaaact tattcttgat aacaagctac gtgacgtata
    45601 tccctaacac aaaaatagcc atatcctttg gtttggatac ggctatttga tattactcat
    45661 tcaattatca aatcacagta ggtgtgaatc tcatcgggat cggcaatatc tacttctgat
    45721 taggatcaaa ggtgattttc tcaataaagg tgtgatacaa agctttaact tgcttcttct
    45781 cactctggat aagaaagcaa tccaaactag tcaatatctg ttggacatct ttgacttgaa
    45841 tcggttcccc ttgttgttga aagatcccta agatttcatt ctctcgaatt tgtaactgtc
    45901 ttcgtttttc agtcaattca tttaaacgat cttttaacat cggaaacaat tcgggactat
    45961 cttccaaagc agcttcctgt ttttgctcta atagttagat tttgcgtacc attttcttct
    46021 tttattttcc ttcgattgct gattttaata tagtctaaag gaaatttata tttattgtat
    46081 tctttataac ttcaagagaa cttcgtttga gtattactgt atttttttaa ttccataaga
    46141 aaattagtta ttacctgcca cctaatcagc aaatctgaac ctgagtcaat ataattaata
    46201 ttattttcca tctccaacat tgtaagtact gttggtgtta atatagggac tttgtacaat
    46261 tgtatttttt tgattttatc atctgcacaa aggagatttt ttaaatctaa tccttctttt
    46321 atttcaaaag ttgaattttt taaatctata agagtaaaac caattccttc atactgaatt
    46381 tctgaaaatc gaatttttct cccagttgat agatcaaggg gcagaaaacc ttttattaca
    46441 ttgtgagtgt caccatcatt tacaatgtca ttaggaattt tgattttaga attctgtgga
    46501 gcacctattg gaagataata attcattata attttgtagt cttcatgagg aaataaagta
    46561 cttttaggat tgctaagata ttctcgtacc gaatctaaga atggcttatc cattgaatct
    46621 tcatccccat ttaaactaaa aaacattcct acaattgctt ttgcaactct aagtgaattc
    46681 attttcttta ttgaatgtgt taaatcagac ttaccattct ttaaacttgt attgttataa
    46741 aggaaatttt tattgtcttc tgcaaatcta atatattcct caacatactc actaccaaaa
    46801 aaactattac atttactaca taacgta
//
LOCUS       DACDXE010000015        46137 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.015, whole genome shotgun sequence.
ACCESSION   DACDXE010000015 DACDXE010000000
VERSION     DACDXE010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 46137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46137
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.015"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(65..376)
                     /gene="rhaM"
                     /locus_tag="IWM23_000851"
     CDS             complement(65..376)
                     /gene="rhaM"
                     /locus_tag="IWM23_000851"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HAP9883514.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(378..1223)
                     /gene="rhaD"
                     /locus_tag="IWM23_000852"
     CDS             complement(378..1223)
                     /gene="rhaD"
                     /locus_tag="IWM23_000852"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HAP9883515.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(1237..2493)
                     /gene="rhaA"
                     /locus_tag="IWM23_000853"
     CDS             complement(1237..2493)
                     /gene="rhaA"
                     /locus_tag="IWM23_000853"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HAP9883516.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(2490..3950)
                     /gene="rhaB"
                     /locus_tag="IWM23_000854"
     CDS             complement(2490..3950)
                     /gene="rhaB"
                     /locus_tag="IWM23_000854"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HAP9883517.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            4103..4972
                     /locus_tag="IWM23_000855"
     CDS             4103..4972
                     /locus_tag="IWM23_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9883518.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(5098..5997)
                     /locus_tag="IWM23_000856"
     CDS             complement(5098..5997)
                     /locus_tag="IWM23_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9883519.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(6162..6986)
                     /locus_tag="IWM23_000857"
     CDS             complement(6162..6986)
                     /locus_tag="IWM23_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9883520.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(6997..8292)
                     /locus_tag="IWM23_000858"
     CDS             complement(6997..8292)
                     /locus_tag="IWM23_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HAP9883521.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(8308..8787)
                     /locus_tag="IWM23_000859"
     CDS             complement(8308..8787)
                     /locus_tag="IWM23_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAP9883522.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(8793..9821)
                     /locus_tag="IWM23_000860"
     CDS             complement(8793..9821)
                     /locus_tag="IWM23_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HAP9883523.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(9838..10944)
                     /locus_tag="IWM23_000861"
     CDS             complement(9838..10944)
                     /locus_tag="IWM23_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9883524.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(11173..13770)
                     /gene="adhE"
                     /locus_tag="IWM23_000862"
                     /gene_synonym="adhC"
     CDS             complement(11173..13770)
                     /gene="adhE"
                     /locus_tag="IWM23_000862"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAP9883525.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(14075..14455)
                     /gene="yajC"
                     /locus_tag="IWM23_000863"
     CDS             complement(14075..14455)
                     /gene="yajC"
                     /locus_tag="IWM23_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAP9883526.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(14552..15697)
                     /gene="tgt"
                     /locus_tag="IWM23_000864"
     CDS             complement(14552..15697)
                     /gene="tgt"
                     /locus_tag="IWM23_000864"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAP9883527.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            15915..16613
                     /locus_tag="IWM23_000865"
     CDS             15915..16613
                     /locus_tag="IWM23_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAP9883528.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(16781..19420)
                     /locus_tag="IWM23_000866"
     CDS             complement(16781..19420)
                     /locus_tag="IWM23_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9883529.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(20039..21070)
                     /gene="queA"
                     /locus_tag="IWM23_000867"
     CDS             complement(20039..21070)
                     /gene="queA"
                     /locus_tag="IWM23_000867"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAP9883530.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            21263..22438
                     /locus_tag="IWM23_000868"
     CDS             21263..22438
                     /locus_tag="IWM23_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HAP9883531.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            22442..23083
                     /locus_tag="IWM23_000869"
     CDS             22442..23083
                     /locus_tag="IWM23_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883532.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            23080..23997
                     /locus_tag="IWM23_000870"
     CDS             23080..23997
                     /locus_tag="IWM23_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9883533.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            23994..24665
                     /locus_tag="IWM23_000871"
     CDS             23994..24665
                     /locus_tag="IWM23_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883534.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(25206..25694)
                     /locus_tag="IWM23_000872"
     CDS             complement(25206..25694)
                     /locus_tag="IWM23_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAP9883535.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(25895..26161)
                     /locus_tag="IWM23_000873"
     CDS             complement(25895..26161)
                     /locus_tag="IWM23_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9883536.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(26161..26424)
                     /locus_tag="IWM23_000874"
     CDS             complement(26161..26424)
                     /locus_tag="IWM23_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9883537.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(26417..26689)
                     /locus_tag="IWM23_000875"
     CDS             complement(26417..26689)
                     /locus_tag="IWM23_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883538.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            26900..27085
                     /locus_tag="IWM23_000876"
     CDS             26900..27085
                     /locus_tag="IWM23_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9883539.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(27227..29050)
                     /locus_tag="IWM23_000877"
     CDS             complement(27227..29050)
                     /locus_tag="IWM23_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAP9883540.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(29648..32353)
                     /locus_tag="IWM23_000878"
     CDS             complement(29648..32353)
                     /locus_tag="IWM23_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAP9883541.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(32659..33606)
                     /locus_tag="IWM23_000879"
     CDS             complement(32659..33606)
                     /locus_tag="IWM23_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAP9883542.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            33796..34065
                     /gene="rpsN"
                     /locus_tag="IWM23_000880"
     CDS             33796..34065
                     /gene="rpsN"
                     /locus_tag="IWM23_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAP9883543.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(34241..34603)
                     /locus_tag="IWM23_000881"
     CDS             complement(34241..34603)
                     /locus_tag="IWM23_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9883544.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(34614..35735)
                     /locus_tag="IWM23_000882"
     CDS             complement(34614..35735)
                     /locus_tag="IWM23_000882"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAP9883545.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(35751..36101)
                     /locus_tag="IWM23_000883"
     CDS             complement(35751..36101)
                     /locus_tag="IWM23_000883"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAP9883546.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(36231..37742)
                     /locus_tag="IWM23_000884"
     CDS             complement(36231..37742)
                     /locus_tag="IWM23_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAP9883547.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(38064..39416)
                     /locus_tag="IWM23_000885"
     CDS             complement(38064..39416)
                     /locus_tag="IWM23_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAP9883548.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(39569..40645)
                     /locus_tag="IWM23_000886"
     CDS             complement(39569..40645)
                     /locus_tag="IWM23_000886"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAP9883549.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            40841..42586
                     /locus_tag="IWM23_000887"
     CDS             40841..42586
                     /locus_tag="IWM23_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAP9883550.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(42866..43303)
                     /locus_tag="IWM23_000888"
     CDS             complement(42866..43303)
                     /locus_tag="IWM23_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAP9883551.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(43287..44660)
                     /locus_tag="IWM23_000889"
     CDS             complement(43287..44660)
                     /locus_tag="IWM23_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883552.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(44982..45470)
                     /locus_tag="IWM23_000890"
     CDS             complement(44982..45470)
                     /locus_tag="IWM23_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9883553.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(45492..46094)
                     /locus_tag="IWM23_000891"
     CDS             complement(45492..46094)
                     /locus_tag="IWM23_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9883554.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        13146 a        10006 c         8333 g        14652 t
ORIGIN      
        1 ttgtattatt tttcacaata aaaacgaaag aaccccaagc aaccggttgc ccagttgctt
       61 gttgctacag ttcaaagact ggcatcaatt cccaactgac tggagaggca tctggatttg
      121 tctccatgat atcttccatg tacttccacc atttttgatt gatagatgta tttgccattt
      181 tttcccataa cttttcatct tcgatcacta agtaaccaaa taacaaactt gtactcggat
      241 caagatagat cgaatagtcg atgaccccgt gttcacgaag tgctgtacgc atttctggcc
      301 aaagttcatc atgtcttttt ttgtattctt cttgtttttc tgggtaaact ttcatgcaaa
      361 aagctttttt caccatgtca gacacctgct ttcgtatgaa cttcctccaa ataacctgct
      421 tttggagtga caccaaacgc atctgctaaa tgccacaggt tttcgtccgt gattgtttgc
      481 aagattggtc cttgcgcttt gacgtacgta tagacttctg ctgccttttc tgctgtctcg
      541 atcaaaccaa agacttcatc catgtctttt cctgacccgt agattccatg ttgtggccat
      601 aaaaccaatc gagtttcttt cattttttca gctgttgctt ccccaatctc atttgttccc
      661 ggaaccatcc acggaatgat ggaaattcct tctggaaaaa caactaagca ttccgtacac
      721 atttgccata gcgtacgact aaaggaacgt tcgtctaatt catgcgtaaa actcatcgct
      781 aatagatgac tagcatggca atgcataacg atccgattat ctgggtctac ttctaaacgg
      841 gcgatatgac tcatgaaatg acttggcaac tcgcttgtag gaaggcattc gttttccagc
      901 ccccacaaaa gttccagtgt tttgccatct tcagcaactc gaacgattcc aagattctcg
      961 gcaggttcgc tcgctacatt tttaaaatat cttcctgaac cagttacaat aaaatagcat
     1021 cctgctaaac ggctggcatc aaaaatcatc gagatgcggc ggatcacttt ttcaggatcc
     1081 aagaacggtg tgatctcttc ttcttttagt aaataagaaa tattcccgcc atttctttca
     1141 tcccatccca agcgataaag attcgacgtc gtttcgatca tttctttcac ataaggcgct
     1201 tgtaaaatat ctattttttt caatatagcc acactcctat tttcttaatt ccaacacgtc
     1261 tttttgatat tcctggactt cttccagcca atccaaacca actggaactt gatttttgta
     1321 acagaaataa ttccatacat cggcatacgg gaaatctttc aattcttccg tataagccat
     1381 tcttgaagta aagtcaaaag ccagctccat ttctttcaat cgctcaactg gttccagcat
     1441 agctttcagc agtgcttttt gtgtatttct cgtaccgatg acccaagcag cgatacggtt
     1501 gatcgttgca tcaaagaaat ccagaccgat atttgttttt tccaacaatt cgttacggac
     1561 taattcttta gcgatttcct gcaattcatc atccatcaaa accacatggt ctgaatccca
     1621 acgcactgga cgggaaacat gcaacatgat tcctttgctg aataaagcta atgaagataa
     1681 tttattggaa atcacttctg ttggatgaaa atgtccagca tctagacaaa caagtttgtc
     1741 acgagtcaac ccgtaaccca tataaaattc atgggaaccg actgtataag cttcagcgcc
     1801 taaaccaaat agtttgcttt ctactgcatc ttgtacatac cgttcatcta actcttctgt
     1861 aaaaatttca tcgagtgatt ccattaaacg ttcccgtggg gtaaaacgat caatcggatt
     1921 atctttcatc ccatcaggta cccaaaagtt attgatactt gtttgcccag tttctttccc
     1981 aaagtactcc gcgattttgc ggcttctttt cccgtgttcg atccagtatt cacgaacttc
     2041 tttatctgga tgagccaatg taaagccgtc tttcatcatt ggatgcgaaa agaaagtcgg
     2101 attgaagtca agaccgattt cttctttttt tgcccactct acccattttt caaaatgttt
     2161 cggctcaatt tcatttagat cgacagattc ttctgtatcc aaatagatag catgaaggtt
     2221 caatttgtgt ttgcccggta tcaacgaata agccttctcc agatcttgtc taagctcttc
     2281 aggtgttcgt gcggctcctg gaaaattccc tgtcgccatg attccaccgg tcaattcgcc
     2341 atccggtgtc aagaaacctt tgacatcatc tccttgccag caatgcatag aaattttgat
     2401 gtcctgtatc ttttctagta cagcatctgt atctacaccg atagttgcat atttttcttt
     2461 tgcttgttcg taatttcttt caatcgtgtt catacgatta ctcctttgat tttattcacg
     2521 taccattcca tcgtgttttg cttattttca tctgggtaaa aagtttcaca tggaaacgac
     2581 tgtttgatta catttcttgc ttgttctata ttcgaaaact gacctgtact gagcatttgc
     2641 atggcaacat tcccgattgc tgtggcttca ctaggtcctg cttctacaat gattccggca
     2701 taatctgctg tcaattgatt cagtagacga ttattcgatc cgccccctac gatgtgcagc
     2761 ttttccaatt tcgttccagt taagtgttct actttgatca gctcttgtgc gtaacaaaga
     2821 gcaagattat catacacaca acgagcgata gctcccgatg agacagggac tggctgctct
     2881 gtttcaaaac agtattgctg aatctcttct atcatatttg ggggattcaa aaatcgaggg
     2941 tcattgattt ccatcacttg ctgcagcggt ttttcttttg cagcaagctc tgccaattcc
     3001 gcataactgt actcgtactc ttgcattcgg gcaacttcct ggatcaacca catccccatg
     3061 atgtttttca aaaaacgcac cgtgttatgg gcaccccatt cattcgtata attttcagaa
     3121 aaagcattgc gagaaacatt ggcgatcttt gtttcaatcc ctaaaagcga ccatgtccca
     3181 ctgctgatat aaccccaacg ttctccttga cctggcgttc ctaatacagc tgacgccgta
     3241 tcatgacttg ctactgtgat aaaggtcaca tctggcaaat catattttga aaattgttct
     3301 ttttgcagat ctcctagtat cgttcccggc tctgtcaatg gtggaaataa ttccttcgag
     3361 acaccgattt tttcaagaag ttctatttcc cattcttttg tccctgcatt cagcagctgc
     3421 atagtagatg cattagtttt ctccgtcacc atgttaccag taaagacata ccctaaataa
     3481 tcaggtatca gtaacaattt atgtgctttt gccaaatttt ctttttcttc agcaaacaat
     3541 tggaacaagg tattgaaagg ctggatctga atccctgtct gctcatacaa cttatctaga
     3601 gaatagattt cagaaaactg ttctactgct ccattggtac gcttgtcccg ataagcaatc
     3661 ggatcattta tccgatcccc ttgttcatca atcagacagt aatcgactgc ccatgtatcg
     3721 atccccgcaa aaaggcgatc aatccctgtc tgctttaatt tttcaaatcc aatcaaaatc
     3781 tctttgataa gataatcgat cttccagcga tcgtgaccgt ctttgttccg aaagccgttt
     3841 ttgaaccggt gtacttcttc caagatcaaa gaaccttttg catccctcgt acttttcatt
     3901 agtcgtccgc ttgatgcacc gatatcgatg gcaacaaatg ttggattcat ttctccacct
     3961 cttttacttt attgttgatt agcactttat taataaagcg ttatcattta ttgacaatgc
     4021 tatcataata cagaatctta tattttgctt cacactattt tggtatataa ttgcattatc
     4081 ttgacaagtt gggagtgttt acatggatta ctgggaaaaa gccaaccact catggtcgga
     4141 agactcaaaa agagtgatct tgacgcctac acaaagaagc aggaatttat ttttttatat
     4201 ccaagaaatc ggttacttca aagcaccaat cccttactat actgaacgag cacatttacc
     4261 ttcttacttg ataaagttta ctttgtctgg caaaggttcg cttacatata atgataatca
     4321 gcaacagcta aaagctggag acttattttt tattgactgc caagaatatc agcgttatca
     4381 aacgatcagc gaagaaccat gggaaatgga ttggatccat ttgaatggtc agaacgtcca
     4441 atcattttat caggagttca caaaagacgg ccataacacc ttccattcaa caggagatcc
     4501 atcagaaaac aagatccacc atttgatcac agaattgatc cacatgcaac aaggcacgaa
     4561 tgcccgtacc gatttccagt cctctgtagc tatccacgag ttgctgaatg aactgatttt
     4621 gcaaaagtat cagctagatt tcgaagaaga ggatattccc gcctacgttt acaaactgaa
     4681 gcaatactta gacgaacatt tcaaagaaac gattacactt gatcaattag aacaacaatt
     4741 cttattgaat aaatatcagc tgagcaaaga tttttccaaa tttatcggtt ctccaccaat
     4801 cgattatttg atcagcaaaa aactttctta cgctaaagac cttcttcggt atacagaatt
     4861 tacggttcga atgatttccg aagaaatagg gattgagaat tttgcttact ttagccgcct
     4921 gttcaaagca aaaacaggac tatcgccgag agactttcgt aaattcagct gattcatata
     4981 atatgtacgt cattttttca aaatattttg aaatatatag ataaaaataa aataaccgca
     5041 gtttctgctt ctatgtaaac aacagcagct actgcggtta ttttttagtg atcattctca
     5101 ttgttcatct tcaaaatgtt cataatgaac caactgtttc tgtgtatcac gtttcaaaaa
     5161 ttctttaggc gttatgccga actctctctt gaatgctttc agaaaatgcg gataagaaga
     5221 aaaaccactt ttactatata cagcactaga gctgcggtat tctcgcaaca agtgcttggc
     5281 atagatcagc tttttcttca ttacatactg atgaaacgta aatccagtgt actttttaaa
     5341 ttctcgtgtg atatggtatt tgctgacaaa aaactgcttt tccatttcct ctaaagaaag
     5401 cggatgagat aaattttttg agatatattg taacatttgt tccacacgct cattaggaac
     5461 tacagcattc tgttcttgcg cactctcttc tttttgtaca acttgattga gaaaaatcaa
     5521 gtattctacc agagcttgtt catattttat gtcttcaccg taaatttttt tatcaggagg
     5581 atggtcgata aatgataatt tttgcttcaa tacttcctgt tctatttgca atacacgact
     5641 cctcttctct cccattggtt gaaaacaagc cgatagatta gaccatggcg tggaacgact
     5701 ttctagaaaa gaaggggtga caaagatata gacacgctcg aatacatctg aagattggcg
     5761 gacgatacga tgcaaatcat tggaatggat gagcagcaca cttcctgctt tgatcgtaaa
     5821 ttgtcgtccg ttcaaataaa aagtcgcttc tccctttaat gtagcatgga tctcatagaa
     5881 atcatgatga tgatagatcg ttttattttg gctgacgaca tctttgacat gatagatttc
     5941 atactgatca gaaaccatcg tattgacttg ttcaccgctg attttctttt ggagcatgtg
     6001 ccgcacctcc ttttgttcac ttatgcacaa ttttattata ccacgttttt tgtaatcaaa
     6061 aagagctgct tttatatact cttataaaaa agaagagacg tggcatcttc tagccccgcc
     6121 ccctccattc taaatattag ttaaccaacg tcttctttac attattcaag aagacttctt
     6181 ccataacgaa tcccatcatc ttttgtttct tcaagaacca catacaggta cggacgattc
     6241 ttcatgatga tttctttgat ttttgtgtac tcgatcacac cgtctcccaa atttaccggc
     6301 acgatttttt cctgttcgac acgaaaatct ttcaaatgaa ccgcacatat cttttcacca
     6361 aaacgttcaa aagcttcttc taccaattgg acttgttctt gataatcact acttgtgatc
     6421 aaattggtcg gatctaaaat gattcctaga tagtccgaat ctactgcttg gatcagctgc
     6481 tctacccttg ctaaagaata caccggatga ttcaaacctg gttctatccc caccatcatt
     6541 tggtattttt ctccagcttt taccaagcga cggataacgg acacagcttc agcaaatgct
     6601 tcatctgtga aattttcttc cgtatattgg atttccggca aaacacatcc agtttccgaa
     6661 gcaaccatcg acgcaccaaa ggaagaagca tagcgaagat actgctcgaa ctttgtcagc
     6721 aactcttgtc gtatcgttaa atctggatga atcatattga tatagcaact taaaattcct
     6781 acggtcactt cttgcttttc taaaactcgt ttgacataat ttcccatccc agaattgatc
     6841 tcatttgcgc cagaaggtat ttcaggaaac gaaagaccta aggcgagctg cagtgtatga
     6901 atcccttgtt cttttgtttt ttctgcaaga tcttctatcg tttggacttc ggaaagatca
     6961 tgtcctctaa ttgtaatgtt caacataact attctcctaa ctcagcatat tgggtagcca
     7021 catgacgatt tgcgggaagt aagtaatcaa aaacaagacg actaagataa ccccataaaa
     7081 aggaagaagc ggtttcagca ttgcctctgc ttttacgcct cctacacttg caccgacata
     7141 tagtcctgta cctactggcg gcgtgatgga gcctacacac agattcatga ttgaaaacaa
     7201 cccgtaatgt acaggatcaa cccctacact tgtgacgatt ggtaaaaata ttggtgtaaa
     7261 aatcaagata gcaggcgcca catccataaa catacccacc agtaataaca caagattgac
     7321 taataacaga ataataaatt tattatccgt tacgcccata atcaacgaac taattgcctg
     7381 aggaataccg gtaaacgcca tggcgaaaga catcatggaa gaagtagcca ataagaacat
     7441 gatcgtccct gacattgcta ctgattgaaa caacatatta ggtatatctt ttagttttac
     7501 cgttttatag taaaacatag caatcaacag ggagtaaatg acagctatcg ctgaagcttc
     7561 tactgctgta aatatccccg tcaagatacc accgataatg atgacaatca atagaaggct
     7621 aggaatcgcc tcttttatta tttttccagc atgagccatc tgtccttttt cgacaacagg
     7681 gtatttattc tttttagcaa cgatgaatgc tactagcatg attcctagcg cccacaaagc
     7741 gcctacgaga taacctccca taaacaaagc agagatcgaa gcaccaccag caacaagtga
     7801 atacatgata aaagcagtac ttggaggaat gaccatacct gtcggagctg acgcgatatt
     7861 gaccgctgtc gcaaacttcc ggtcatatcc ttcttccact tgctgaggga tcaacactcc
     7921 gccaatcgcg gtagaagcgg caatggccga gctagagatg gaaccaaata aagcattccc
     7981 taacacattc gtatgagcca aagcccccgg cacgcgcccg acgaacaaca tcgcaaaatc
     8041 cactagtttc ttagctatcc ctccattatt catgataatg cccgagagaa cgaaaaacgg
     8101 aatagcgatc agcgaaaaac tattgatact tcccaccatt ttttgagctg aactaaaagc
     8161 agaaacatca aacggtataa ctaacaataa agtcatcatc gatgagaatg caatacttat
     8221 agcaatcggc atccctaata caagcaacac aacaaataca aaaaataaca ctaatccggc
     8281 ttctattgcc attttattcc tccattttta tattttttct tgaattgctg gcttgttgct
     8341 taatgtatcg attagattga tcaggctata gataatgatg agtacccccg atacaggtac
     8401 tgacagataa acatatccca taggaatact taaagaaggc gagatctgcc ccattgtcaa
     8461 tgtgacagca ttccatccgc catacaccat gatcatcgca gcaaatacca gaaataaact
     8521 ttgaacaaca acttctaaaa ttttactttt tttgccagtt aatttttcac ttaaaaccgt
     8581 tacggctaaa tgtgcttttt gtcccacaac atacgcggaa gacaacatgg caaaccagac
     8641 aagaccgaat cgaacaagct cttccgtgac cgtatttgga tttcctaaaa ccgttctgga
     8701 aaatacttgc cataaaacaa cagcgaccat cccaacaagc aaaacagctc ccaggatctc
     8761 taataccttg tttaaaatca ttttcatcac agctacgctc cttgctcttg gatcttttga
     8821 taaaggtctt tgtaggtagg atcgtttttg aatatatcat gaagtggggc aactgcctct
     8881 cgaaacggtg tattatctac atcattgaat tggactccat gttcttcttg tgctaaagct
     8941 ttttcttctt caattgcttt tgcaaaagct tcaatttcaa aaaccgttga ttctttcgct
     9001 gcttcttcaa tggctttttc ctgctcatca gttagctgtt ctttgatggc atcattcatg
     9061 atgatgatat ctggcacacg tgtatgctca tcataggaat agtatttagc cacaccacca
     9121 tgtccagctg tatacaagac aaattcattg ttttctgatc cgttgatcaa attggattga
     9181 agagacgtgt acacttcatc acttcccata ggtacgggcg aaccgcctaa aagctctacc
     9241 attttgatag cggtttcgct ttgcatcaca cggatcttct ttcccttcaa atcttccggc
     9301 gtatggatcg gaccgtctac catataaaag ctgcgctgac cagaatcata ataggtcaat
     9361 ccggtaaatc ctaaattttt ggtcgattgg tagattggct ccattatttc ttcattatcc
     9421 atcaccttaa agaaatgttc ttcatcattg aatagataag gaactgaaaa aatcgaataa
     9481 tctttagaga aactttccaa cgctgaggca cttacttttg tgaaatctac tgctcctgac
     9541 tgtgtcaatt cgatcagttc acgttctcca cccagttgtc catctgggaa atactggatc
     9601 tgtacggaac catctgtttt ttcttttact aaacgcccaa attcattcat tgcatcaatt
     9661 actggctggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacatctgca
     9721 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     9781 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     9841 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     9901 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     9961 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
    10021 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
    10081 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
    10141 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
    10201 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
    10261 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
    10321 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
    10381 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
    10441 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
    10501 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
    10561 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
    10621 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
    10681 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
    10741 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
    10801 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
    10861 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
    10921 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
    10981 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
    11041 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
    11101 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
    11161 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
    11221 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
    11281 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
    11341 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
    11401 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
    11461 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
    11521 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
    11581 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
    11641 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
    11701 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
    11761 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
    11821 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
    11881 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
    11941 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
    12001 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
    12061 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
    12121 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
    12181 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
    12241 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
    12301 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
    12361 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
    12421 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
    12481 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
    12541 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
    12601 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
    12661 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
    12721 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
    12781 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
    12841 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
    12901 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
    12961 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
    13021 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
    13081 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
    13141 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
    13201 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
    13261 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
    13321 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
    13381 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
    13441 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
    13501 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
    13561 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
    13621 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
    13681 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
    13741 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
    13801 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
    13861 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
    13921 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
    13981 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
    14041 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
    14101 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
    14161 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
    14221 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
    14281 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
    14341 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
    14401 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
    14461 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
    14521 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
    14581 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
    14641 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
    14701 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
    14761 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
    14821 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
    14881 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
    14941 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
    15001 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
    15061 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
    15121 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
    15181 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
    15241 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
    15301 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
    15361 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
    15421 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
    15481 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
    15541 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
    15601 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
    15661 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
    15721 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
    15781 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
    15841 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
    15901 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
    15961 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
    16021 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
    16081 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
    16141 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
    16201 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
    16261 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
    16321 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
    16381 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
    16441 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
    16501 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
    16561 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
    16621 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
    16681 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
    16741 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
    16801 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
    16861 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
    16921 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
    16981 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
    17041 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
    17101 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
    17161 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
    17221 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
    17281 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
    17341 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
    17401 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
    17461 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
    17521 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
    17581 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
    17641 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
    17701 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    17761 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    17821 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    17881 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    17941 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    18001 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    18061 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    18121 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    18181 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    18241 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    18301 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    18361 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    18421 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    18481 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    18541 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    18601 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    18661 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    18721 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    18781 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    18841 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    18901 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    18961 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    19021 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    19081 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    19141 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    19201 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    19261 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    19321 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    19381 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    19441 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    19501 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    19561 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    19621 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    19681 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    19741 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    19801 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    19861 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    19921 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    19981 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    20041 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    20101 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    20161 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    20221 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    20281 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    20341 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    20401 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    20461 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    20521 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    20581 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    20641 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    20701 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    20761 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    20821 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    20881 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    20941 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    21001 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    21061 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    21121 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    21181 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    21241 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    21301 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    21361 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    21421 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    21481 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    21541 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    21601 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    21661 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    21721 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    21781 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    21841 ccatgacatg gatgaagcga ttaaactagc aagccgtatc gccatcatga gtgaaggccg
    21901 tgtgatccaa tttgatacac cagaaaatat tttacgtcat cctgcaaacg atttcgtaga
    21961 agagttgatt ggagaagatc gcctactaca agccaagcca aatgcgacaa gagtcggcga
    22021 agttatgcta aatacagcaa tcacgattac accggaaaaa tcattacaag aagcaatcaa
    22081 actgatgagg gaaaaacgtg tagatacgtt gttagttacc gataattcga atgtattaaa
    22141 aggatttatc gatgtagaaa cgttgaataa aaaacgagga aaagtatcca gtgtcggcga
    22201 cattttgaat aaacatgttt tttacgtaaa agaatccgcc tatcttcgtg ataccttgca
    22261 acgcatttta aaacgcgggc tgaaatacgt gccggtcgtt gatgaacaga acaaagtcgt
    22321 aggtatcctg actcgtgcat cattagtgga tatcgtctac gatgtgattt ggggagaaga
    22381 tcccgcaact actgatgttg cagagtcagc tactcctgaa gaatctaagg aggtctgacc
    22441 tatgcagcag ttttttcatc agcacggttc agaaattctc tcaaaaggtt ttgaacatct
    22501 ttatatttca ggacttgctt tgatcattgg tatcatcatt gctgtaccgc ttggtgtctt
    22561 actgacacgt tttccaaaag ctgccacgat tgtgattggc ttaaccagtg ccttgcagac
    22621 aatcccttct cttgctttat tggcattgat gatcccgcta tttggcgtag gtaaattacc
    22681 tgcgattatc gcactcttta tttattcatt attgccgatt ttacgtaata cgtacattgg
    22741 gatgaaaaat gtcgattgga attatcggga tgtggcaaaa ggcatgggga tgactgagtt
    22801 ccaatccatt ttttccgttg aattgcccat tgctatgcca acgatcatgg ctggtattcg
    22861 tttagctgcc gtttacgtga ttgcatgggc tacacttgcc tcttatatcg gcgctggtgg
    22921 tttaggcgat ttgattttta gtggactgaa taattaccaa ccagatctca ttttggcagg
    22981 tacgatccct gtaacaattt tagcactatt ggctgattgg cttctaggtc tcttggaaca
    23041 ccggctgaca ccgatcgcac ttagagagga gaatgaagaa tgaaacgtat actaaaaatg
    23101 accatggttc ttttctctct tcttatactt gcgagttgtt ccttccctgg tcttgccagc
    23161 aatacagatg aagacaccat ttcgatcaca ggtggaatca cttcagaagc acagatctta
    23221 gcaagtctag ttgccggaat ggttgaacat tatacagata aaaataccgc aatcatcaac
    23281 aatctggcta ctacaactat caaccatcaa gcgatgatga acggagatgc ttcaatttca
    23341 gcggctcgct acacaggaac agatttaacg actacattga accttcctcc tgaaaaagat
    23401 ccgaaaaaag cgtttgctac cgtgaaagat gaatttgaga aaagatacgg tcaaacgtgg
    23461 ttcccgtcct atagttttga aaatacgtat gtctttcttg ttcgaaaaga caccgcacaa
    23521 aaataccatc tatctaaagt cagcgatctg aaaaatgtag cggatgaatt agtagctggt
    23581 gtagatactt cttggatcaa tcgtaaagga gacggttatg acggcttcca agaaacttat
    23641 ggcttctcct tcaattcgat attacctatg cagattggac ttgtttatga cgcagtcgaa
    23701 gcaggaaaaa tggatattgt tcttggctac tctacggatg gacggatcgc cagttatgat
    23761 ctagtcatgt tggaagatga tcgtcaattc ttcccaccat atgatgctgc tccagttgta
    23821 gacaacaaga tattagctga tacgcctcat ttaaaagaag ccttaaataa gttaggtcat
    23881 accatcagta cggaaaaaat gcaggaattg aactacgaag cagataataa tctagtggaa
    23941 ccatcagtag tagcacaacg tttcttacaa gaaaaccact attttgaagg gaagtgaagc
    24001 aattggataa aatgaatgtc tttcaacaat ttttctatta tttccaggaa aatggctcct
    24061 atatctttgc acaatttatc cgtcacttcc tgatttctat ctatggtgtc ctctttgcag
    24121 cagttgtcgg gattccggtt gggatcatga tttctaggag acgtaaatta gcaaactggg
    24181 tgattcgttt ggcgaatatc atccagacga tcccttctct agccatgatc tccattttaa
    24241 tcattggatt aggcctggga gtcaatgtcg tgattgttac tgtatttttg tattccctct
    24301 taccgatcat caagaatacg tataccggga tgatccaagt agacaaaaac atccttgatg
    24361 tcggcaaagg catggggatg actgctagac aacgactatt catggtagag ttaccactct
    24421 ctgtttctgt tatcatggca ggcataagaa acgcgttagt tgttgccatc gggataaccg
    24481 cgatcggtgc attcgtcggt gctggtggtc ttggagatat cattatccga ggaaccaatg
    24541 caacagaggg cacatcgatt attttagcag gtgcattgcc aactgcatta atggcgatca
    24601 tcacggactg gcttttaggt attttggaaa gacggctaga tccagccagc cgtacatcac
    24661 gctgatgtta ttgtaaaaaa caaaagcttc tgcgaccatt tgttggtcgc aaaggctttt
    24721 ttagttctgt catttttaag gcaataatcc actctgattc ttccctcctt atgggtaaag
    24781 agttgctttt cttagctatt tatccattcc gtatactcca acttcatact gccaacgggt
    24841 tcatatctgc atccaccgac taagtttttt tcttatccgc taaggcattt tgccaatcca
    24901 ttttcccatc actcgtcatg gaattcatac cacatgatct ctaaatactc gtctgcttgt
    24961 tctttgaatt gggcatcaat tcttacatcc tttggcgtgg ctttcattta agccttttcc
    25021 atcagtcctc gattgttcga tgccttcagc tgcatcatca aaactggtga ctgccgtatc
    25081 ggtaacttcg atcatctttt atacaatcgg gaacttgcca tcctcatcct tggctaaaaa
    25141 catatctagc cattccagct tctcgccatg ggaggtttgc ggttcctaaa accggtacac
    25201 gattcttatg gactgcatcc gtcgcaacta gacttggcga tacgatgatc cttcaccaga
    25261 agagcacccc cgtaaaccaa ttgatcgata tattgccaat aagaaaagac attggcgtaa
    25321 aagatatcga tccctcgtga tgcattgccg ttgatactgc tattcatggc cgaaagcgcc
    25381 acgactttaa cttcttccgt ttgagtgaca ttcatgttcg gcaatgtcgc tttggatacc
    25441 cgctctgcta aaggaacgac actggtattg tatttcgtat ctgtatcttt tccaaaagac
    25501 catgccaacg aatcctctgg gaaccaataa gacgactccg gttgattgtc cattgctttt
    25561 ttcaacatac ttttggctgt ttctcgataa acgacatttc caattgtctt cgcctcactt
    25621 tggcgataat cgatggtcgt tgccactgcc aacactaaca acgatgctcc aattttactg
    25681 attttccttg tcacacttct tcctccttgt tttacataaa cccgtcaaca ttccattcct
    25741 aaactcaaac aatgaccctt caaaggtgga gatataggaa caaattaaag aaatcatgca
    25801 ataaatccaa gaaggttggt tttagaacaa aaaaagagtc tcctttttgg ggagactctt
    25861 tgttaactat ctaaaaaggt tggctcttct cacttcactc ataatgtgtc caggcagaat
    25921 aaatttctac aactttattt tcctcatcaa ctttgtaaac cactcgatgc tgtctattca
    25981 gcctgcgtga gtatctgcct tcatgtgtag gtctcagctt ttcaaaagac tgcgtgggca
    26041 tatatggatc ttccttcaat gtctgaatca cttcttcaaa ttgtttcttt aaattcgttt
    26101 gcttgatttt tcttaaatcc actttcgcag agtttttgat tgcgactgta tagttactca
    26161 tcaaagagct tcccaatcaa tatcatcgat attcgtaaag ctactgtcgt ctttttcgcg
    26221 gtctcgtact ttatctaaag tacctgtttg ctcaagaaaa agggtttctt gaatcgctct
    26281 ccagtcttcc agcccaatca atacagcatt gtttccacta cgatcactaa taatctcgat
    26341 ttcagtatga ttttcattta cctctttcaa taactgataa aaatttttac gagcagtgct
    26401 aggattcatc gttgtattag ccattgtctc tcctcctttt tctgatataa ttatagcgta
    26461 cttgattaag tacgtcaaaa aaatacaata aagcacttaa attcagcaaa cttttcctct
    26521 tttttacata gagaaaaaga ccccttttta gggaggatct ttgtccacgc tcacttctta
    26581 ctcctttacg actagcggta gccaaacgta aagtaattgg taacaacgta tcatctttag
    26641 caaaggtcga atccaaagcc tgtgagtcta tgcaacaaca ccaatacacg aaggttcctc
    26701 gttgcagtgt gacttgattg atatcttgaa taacctgagg atacgcacga aagatttgta
    26761 acggttattc aaaactgatt ttggccgtca agtcttctac tgcctcaaaa atgattgcct
    26821 ctttcgtctc ctgtcattca tgttcactat cgatttgata cttgcttaga ccgttgggtt
    26881 tgtgaatcac caattaaaaa tggcactgag ttatggtaac aaaaaatggt cacttgacga
    26941 ggctgagatt cctacttatg aagacacatg gcacttaaga gtcgaggtaa aggaagaagc
    27001 tgaaaatata ggaggctgta cattatttgg caaaggattc ggaaattatg atcaagacat
    27061 caaaatcaaa atgttttaca gctaaatagg aagaataata acatttcata cgctgttccc
    27121 atctttaatg gcttatagag ggcaccttta gctgagaata agacaaaaca gaccctctag
    27181 cttcaattaa gagctagagg gtctgttttt gattttaaaa cttggtttat ttcttcaaat
    27241 ggtagctata ggtgctgaca acgatatttt cacgccaaga caatcgcaat cttaaccgca
    27301 aacttgtttg gttatggaga atattctgcc agcgtctgtt tggtacaaat tgaggaatca
    27361 acacagtagt tgtatagttt tgtttcgctg catttttact tacgatatct acataacgga
    27421 taattggatt ttcaatcgag cgataagaag agtggacgat tgaaaaacgg acatctggga
    27481 aatgcttttt aaattctgct tcgatttctt tttctttttc gacatcttcg tctagcgaaa
    27541 catgcatcgc aacgacataa tcaccgatcg aacgtgcgta attcaacgca ccgatattta
    27601 cccgtgtcac attcccgact aatacaagaa cggtattccc gtcataatca tgtagttttg
    27661 catcattttc taaacgaagc tgttctgcta cttttttata atgatcatgg atcttataaa
    27721 aaatgaacat aacgattggc ataatgatga agaaaggcca aatgtcccct aaccgataga
    27781 caaacaaaat cgcaatgatt gcatatgaga tgaacgcacc cgtaatattg gcaatcgatt
    27841 tgcttaacca tctttttcct tcttttttcc atttgacgac catccctgtt tgagataaag
    27901 caaatggaat gaacacgccg atagagtata acggaatcaa gcgttctgtt gagccatgga
    27961 aaataaacag caatacgatc gaacctagcg ctagtgtgat aatcccattg gaatagccta
    28021 gtcgatcccc acgatcttga tacatgtgag gcatgaattt atcttttgcc agattgtatg
    28081 caagaactgg aaaagcagaa aaaccggtgt tagcagcaac tgctaaaatc agcgctgtag
    28141 caaattgaag gacgtaatac aaaataccat gtccaaagac agctttacca atttgagaca
    28201 aaacagtgac ttctttttca ggaacgatcc cataccaata gttgataaat gtgatcccca
    28261 cgaagaagaa acctaagatc aatgccatca tcgtcaatgt agcagcggca ttttttgctc
    28321 ttggcttctt aaagaatggt acagcattac taatcgcctc caccccagtt aaagaggaag
    28381 aaccagaaga aaatgctcgt aaaatcaaag cgatagaaac acccggcaca gccgctcctg
    28441 gaagtgcagt agcttgcaat ggcacagcac ctgtcacgat attatataaa ccaacaacga
    28501 tcagtaacga gatcacaagg ataaatgtgt acaccggaaa taataggaac gatgcagatt
    28561 ccctcagccc acgaagattc agcatcatca gtagcaaaac gattgtcaca gaaatagcga
    28621 cttgatgacc gtagagtgct ggcaccgctg atgtgatcgc ttcagcacct gctgagacag
    28681 atactgctac tgtcaacatg tagtcgatca ataaagagcc accggaaatc aagccagcat
    28741 tctttccaag attctcactg ctgacgacat atgctccccc gccatgagga taagcatgga
    28801 tgatttgacg atatgatagc gttaatgaaa tcaataaaat aataacaaaa gcggcaatgg
    28861 gtagcgaata ccagatggct gcagcagaca aagcgactaa aacgacaacg atttgttccg
    28921 taccatatgc aatagatgat aacgcatcag acgacaataa tgccaaagca gcaaatcggg
    28981 ttaatttgtg ctcatcattt tcagcagatt ttaaaggctt acctactaac aaccttttta
    29041 agtaatccaa tttctaaact cctttacaca caattttact tcaagtttta tagtcgaaaa
    29101 tttatagtaa acaagatgtt atagtacgct cactgacctt aaaagtcaat aaactttaag
    29161 aaaataattg gaaaaaaaca gaaaaatatc aagcgctttt tcaaacgctt taatacagca
    29221 aaaatctaac atttttttat gggctttttc tttgaattct ataataaaag gatagaactt
    29281 gacttagaaa atcaagttct atcctttttg atatcttaac ttttggaaga gagatgtaag
    29341 gctgctttct acccatcatt cttactactc gaagcatagc gcagttgtca caacctcttg
    29401 ataaacggtg tttaaaagct tgcatctgcg gtaataagcc caaccaaatt aaaaacataa
    29461 gaagctgttt ttatttggtc gttcttatta cttgatgcag agcagctttt ttacaacctc
    29521 ttggtacacg gtgttcaatc agctgaccct cggtattagc tcaaaaaaac ggcaaaatat
    29581 gaggaactat ttttccattt ttttctctaa tactcaggtc aaaacactga tttcacaacc
    29641 tcttttttta atgaaaaata tggctctcat gcgccgcttc tttgaatttc gtcatttttt
    29701 cttcgatgaa cggatagatt gccgttccta cgattccaaa gacaacaaac aaaccgatca
    29761 tgatccagat tgctttcgta gcatttggcc aataaattcc tcctgcagat tcccgtaaca
    29821 agtttaccgc atgagtaaat ggcaagaacg gattgatcat ttggaagaat ttccctgaga
    29881 cttggatcgg atagttcccg cctccgccag agatcgacag tactaagatg ataatagcga
    29941 tccctttacc gacattatcg aataatgcag ccaagacata gacgatcatc atgaacgcta
    30001 aggcgatcaa gacagcaaat gacacgctgt agaccggatt ttggacatct actcctagta
    30061 agaaaatatt ccctaatgta acgatcaagc tttgcgcaat cgccattgtc aaaaatgtca
    30121 gcatacgagc aacgaattgt tctcgtttcg taaaattgtt tcggtctttc tttcctaaat
    30181 agtattctgt cgtagcaaca cttgaaagaa gaacagcgcc gacccacaga catagtgcag
    30241 tataaaacgg ggtgctagct gaaccgttat ttgcgattgg ataaagcgca ttggtttgta
    30301 gctcaacagg ttgtgtaaag aagtcacttt cttttgttgc gtctagtttc aataatttga
    30361 tgacttcgcc tagatcgact tcttgttcgc cttttcggat cgcgtctgct gctttttgga
    30421 taccagcttt gatatttggc cagtcattgt tgatcaagtc cgcagcagcg ttcacagctg
    30481 tttcgacttc tggcattttt tcatttactg ttgtcaatgt accggtgatt tcatttttta
    30541 gtgctggcca atcattttgg ataaaatcag cggccaatcc taatttttgc tgaatgatcg
    30601 gtaattcatt gttgtatagg tctgcccctt cattgatgcc attgacgatg gtatccatat
    30661 ttccattcag cattgtattg gcatcatgga tttcttgtcc aattgcaggc atttcttttt
    30721 gatatttttc taaaattgcc actgcattcg ttacagttgc tttcgttgag ctgagaagcg
    30781 aatcaaagtc gatctggtcc gctttgctca gtgcgccttg tgcagtccca atcgtatcga
    30841 tgacttggtc tagtagagaa gtgacagtgg aactgatttg acctgtattg ataccagcaa
    30901 tgatgccatt gatattatta acaacggcat caatttgatc tagcaattca tcaatagcct
    30961 tcgtgtcttc attttgaaca gctgtgttca aagcagatag gcggttttgc agattgctta
    31021 gcaagggttt taattgattt agctgatcaa taattggctg taatttcgta tttccagcac
    31081 ttgtttgcaa tttagtcagg aactcgacca gtttatcgat tgcattttgt tgttgagata
    31141 ttccgtcaat aaagccgttc aatacattgc tgatgttttt cttttcatca tcagtcagct
    31201 taccatcttc aatagctgtt cttacagtat ctattaacga acctgtcgtt gttgccactt
    31261 gtccaattga cttcaatgta acatctacac tgtttgtgat gcttggtatc gcatctttca
    31321 gctgagaggc acctgatttt gtttggtttg ctagatcact tgcctgattt tctaaatttt
    31381 tcacatctgg caagatatct tgtacttgtt ggatgatcgt caagccttgt tttgcttcat
    31441 cgattccttg gttcattgtc tcttggatcg aagcaaaatc gccgtcgatc tctgctagtt
    31501 gcttaccggc attttggatc tctgggattt tttcttgcaa atcaaggatg atttttgctt
    31561 gatctttcaa ctcaggcatt ttgtcattca atgcaacgac tttttcaccc atctcatcta
    31621 ctttagggat gtaatccacg aattcatttg ctttattcag tttttccttg atttcaggta
    31681 gttgactttg taattcgagg acttgttttg tgtagccgtc gatcgtgtcc aaattttcat
    31741 ctgttgataa aatcatatct ttaactttat tgatacttac taggttcgta tcgatgtcgt
    31801 aacctatctc attgaatact ttcaataaag tcgaactggc tgttttgata aattcatttg
    31861 tgatttgcga ttggatcgaa cttgctcctt tatcggtgat tttcggtgca atcgcattga
    31921 ttttttcatt taccgtatat tcgatttttg gtttcttgat atcaccacta gtaaaactca
    31981 gcagatcttc tgagaaatcc tttggcaagt aaatacctgc gtaatacttt ccggaacgga
    32041 ctccgtcttc taattgttct tttgaatcaa cgaattgcca tcctaattgt ttatttttat
    32101 gaagcgattc gatgacttgc tccccaatgg ccacttcttt accttgaaac tctgctggct
    32161 tgtctgcact gtaaacagca attggcagct ctccagtatt tccatatgga tcccataatg
    32221 ctttgatgtt aaaccacgca tatagcgaag ggatgatcat gatcgcgatg atcagaagtg
    32281 ttgcaatcgg gtttttgaaa atccgtttcc agtcaagcaa atataatcga agcgtatttt
    32341 tgattgactg catagttctc ccccttcttc ttttttcagc tgaattactt taccatgtat
    32401 ttcatacttt tttcaatcgg cttttttcaa aaaagtcgaa tttttggaca tatcacggca
    32461 attgtctcat tgtaatctct tacatattga gaaataatta aggattacag cctttacttt
    32521 atcatgaaat aaaagggaaa gataatttta tgccaaagtt tttatctatt atacgtatac
    32581 gcaaaaaagc taaaacattt tttgtctttg cccctacaaa aatgtttcag cttttttcgt
    32641 tttttattcc tgtatttctt atttttcgaa tgacaggatg attttcccag caggaggatg
    32701 tccttcaact agctgatgtg cttggatgac atgcttaagt gaagcagaca gattcattga
    32761 aataaaaaca tgaaaggctc ctttttggta ggctttgatt atgccagcaa aggcttctgc
    32821 atctagatat tcctttcttg gaacaaacga ttcataaaag ccttcctttt tttgccgcaa
    32881 atctagatct gggaggtcat ttaatgcaac atacctgccg cctggtttca tgatctggat
    32941 gccggtttct cctttgatac ttcctttcgt cgcatcgatt acaaggtctg cctgatcagc
    33001 aaattgcaga cctggatttg ttttatcata agcagcaaat gctgaagctc ctaatttttt
    33061 taccttttcc tcatttttac tggatgcact ggttaagatt cggattccct tttcatggag
    33121 aagctggatc agacttgacc cgaccgcacc tgatgctcct agtatcatta cagtatctgt
    33181 cggctggatt tccgtcaaat ggttgattag attataagcc gtaattcctg gcgtgaccat
    33241 tccggctgct tcttcccaac tcattttatc tggaattttt gctacttttg ctgatggcaa
    33301 cacgacttct tctccgtaag tcccgccgac tgcatggaca gctactcgat cacctacatg
    33361 aacagttgtg acatctgaac caatctctgt cacaatacca gcgccatcat tacctggtac
    33421 ataagggaat ttcaatgttc gtatctcttt catttctcct aaacgcaagg caacatcata
    33481 aggattcaca ctgaatgctt ttatttctac acgaacatgt ccagaaccga cttctctggg
    33541 atgagcatcg atttcttcaa aaacatcttg tgcttctccg taaccgttaa ttgcaaatct
    33601 tcgcatggtc accatctccc tttttcttta agtttacgtt tatgccttta ttttgacaaa
    33661 aaaaatgtct tttcgttttg gcgcatgatt caaaacaaaa tagttgtctt ttttcgcgta
    33721 acgtgttaaa ttatttatct aaaagcgtaa agattacgat ttacgctaat aaaaaataat
    33781 tggaggaatt tgttgatggc taaaaaatca aagattgcca aagcaaaaaa acagcaagaa
    33841 acgattgaac gctatgctgc tttacgccag gaattaaaag cgcaaaaaga ttacgaaggc
    33901 ttgcgtaaac ttcctttaga tgctcgtcca gaacgattga aaaatcgtga tttaatcgat
    33961 ggacgccctc gcggatacat gcgtaaattc gggatgtccc gtataaaatt ccgtgaatta
    34021 gcacataaag gtttgattcc aggagtaaaa aaagccagct ggtaatccaa gtctggctgt
    34081 aatgtaaagg aggagggcgc atggaccact ccttggatca tcaaaaaaga tacgttcgct
    34141 tcttctagca gaaatgctgg tactagaagc agacgtatct ttttttacgc acagctggtc
    34201 cttttttata tgtaaaggct gcatggttac ttcgctatat ttatttagta acaggagtca
    34261 gtgcttcgat acccacacta attttcaatg ctcggtcaac ttctgccata atgggtcgat
    34321 ccaaatggca gaccttttct ttcaatcttg atttatcgat cgttctgatc tgctccaaca
    34381 ggataacgga gtctctttct attcctgttc cagtagcttt gatcccaatg tgagtcggaa
    34441 gcttaggttt agccattttt gccgttatgg ccgcaacaat gatcgtcggg ctaaaatggt
    34501 tacctagatt attttgaata accaaaactg ggcgtgttcc gccttgctcc gatcccacga
    34561 ctggagaaag atcagcaaaa taaatatctc ctctttttac cataggatcc tccttattct
    34621 tgatattctc ttggtatccg ctgtcctaat ccgcaagcca cttcatagtg gatcgtacct
    34681 aattgatctg ctatatcctg catagtgatt tctttgtctg catttttgcc gataagcgtc
    34741 acttttgttc cgacaggaaa ttcttgaggc aatcggatca tcagttgatc catacataca
    34801 cgcccaatcg tttcacagta gttcccttct accaatagag aaaaaccctg catctttcgt
    34861 ggccagccat ctgcataacc gatcggtatc gttccgatcc actctgcttc aggagtgata
    34921 tatgtttctc catagccgat tccttcacct gctgggagtt ttttgacttg aatcaattca
    34981 gaaaccagct caagtgctgg ctgcaatgga taaacttccg gtagtgcgtg cccagaggga
    35041 ttaagtccgt acattgctac tccataacga atcatattgc cgattgtttc atcatgccat
    35101 aaagccgttg cactattgct gacatgtaca tatcgaggaa gttcgggaag ttttttcagc
    35161 acttctataa agcgttcttt ttgcagattc caatatgtat catctgcctg atctgctgta
    35221 gaaaaatggg taaataaacc ttcccattct aatacacgac tttctttgat gaatgcagct
    35281 gcctctttta cttcttcagg tgtacaaaat ccgatacgac ccatcccagt gtctgctttt
    35341 agatgaatcg aaagaggcgt ctctagctta cttttctcta aaacagcagc tgctgcttgc
    35401 aaccattccc gtgtacctgc agttactgat aaatcatgag caacagctac agtcagtgcg
    35461 tcgtaaggaa ccatattcaa gataaggatg ggctgagtaa atccagcttc tctcaactcg
    35521 actccttcat ctaaatttga aacacaaaag cccgaagctc cacctgcaac tgctgcctca
    35581 gctgtttcaa tagctccatg accgtatccg ttcgctttta ctactgcaaa caattctttt
    35641 ccttgcggaa gtcttgcaac ttcatttgct acattttcag ttatagcttt tttatgtatc
    35701 actgctttcg ttgggcggtg ccaagcgaca accatcgttt ttccttcttt ctagtcttcc
    35761 aaaataattt gtgcaaaagc tgtttcattt gtatgagtaa tactgaccca tacattgcct
    35821 tcatgaggtg aacgcgtcac aacaggctga ccattttcat tttttaaaat ctcgacatct
    35881 tgaaaggtaa cttttccgat cccagttccc caagctttag agaaagcctc tttgcaagcg
    35941 taacgcccgc ctaaaaattc tacttggcga tgaaaaggca aggactggaa tagtttcatt
    36001 tcatcagagg tcaagattcg tgcaatgaat tttggttttt cttcgatcag ccgagtgatc
    36061 ctaggaagtt cgactgcatc gatcccaatt ccttttatca tatgcttttc tcttttcaat
    36121 aaatttagaa cactctcatt ctaccaaatt ataaccattt tgccacaaat aagtccacaa
    36181 ataacaaaaa tgaaagtttc tcttaaaaaa gagaaacttt cattttatgt ttaatcgtta
    36241 tttgaacgga ttacaaatcc gcgtttttta tcattgtgac gtttaccgcc atctttttgg
    36301 ctgttattat ttttgctata accgcctttt gatttgtttc cacgataatt accgccgtca
    36361 cgacgattac ggttgtttcc gccaccacgt ttgccatttt tattgtatcc ttttttctgc
    36421 attggcaatg ggcgttctgg tgtgatcttc actggcactg catcagctgg atcttttgct
    36481 gtcgttttta gcaaaagtgc aactaaatct tgtgcagaat attcttctaa taatttatct
    36541 gctgtttgca ggtatttatc tagaccattt tctgcaagtt ttgtttcgat ttgttcaact
    36601 gcagcaccta attgaccttt gaatgcttct ttttcggttg gtggacgtaa agtcgtcatc
    36661 cgttttttcg tcaagttttc aatcacatgc aagtagtcca tttcatttgg tgtaacgaaa
    36721 gtcacagaca taccgccttt tccggcacgg cctgtacgtc cgatacggtg aacgtagctt
    36781 tctggatctt gagggatatc atagttgtac acatgagtga cacctgaaat atctaatcca
    36841 cgtgcagcaa cgtctgtcgc tacaaggata tctaaatgac cgctcttaaa tgaacgcaat
    36901 acgctcattc gtttttgttg agacaaatcg ccatggattc cttctgcacg gtaaccgcgt
    36961 gcttctaatc cgcgtgccaa ttcgtctaca cggcgttttg tacgtccgaa gacgattgtt
    37021 aattcaggtg tttgaacatc aaacaagcga gtcatgatat cgaatttttc gtattctttt
    37081 gcgcggacat agtattgatc gatcaagtca gctgtcattt ctttcgcttt gatcttcaca
    37141 tgatgtggat ttttcatgaa tttaacgcca atattcttga tagctggcgg catcgttgct
    37201 gagaaaagta atgtttgtct ttgatcagga acttgtgaaa tgattttttc gatatcttcc
    37261 aagaagccca tattcaacat ttcatctgct tcatccaaaa ctaatgtttg aacggttcca
    37321 agtttcaatg tgtggcggtt aatgtggtca agcatacgtc ctggtgtacc cacaacgata
    37381 tgtggacgat ctttcaagcc acggatctga cggccaatat ctgctccgcc gtaaacggct
    37441 tgtacacgta cttttttgtc gcgccctaaa cgatataatt cttcttgtgt ttggatagct
    37501 aattcacgtg ttggtgcgat aactaaacct tgtaattcgt gacgatctgg atcgattttt
    37561 tctaacattg gtaatccaaa tgcagctgtt ttccctgtac ctgtctgtgc ttgtccaata
    37621 acgtcttttc cagctaatgc tagtggaatc gtttctgctt ggataggcgt tgcttcttca
    37681 aaaccagcgc gttcgactga tttcaacaat tctggtgata attctagttc tttaaatttc
    37741 aaatgaatcc tcctatatgg ttgttaactg aggatgtagc ttttttgcta ccccgtcgat
    37801 ttgttcggtc aaagtgtatt gtttcttttt gacagtggcc acgcacgttt ttatccattc
    37861 ttagcccctg tggcaccacc gaaaaatcac tttgcgaagt ttaggcatta gaaaagccaa
    37921 acgggtcgcg ttcagcttta tcattattat gacatctggc ttcaacaagt tgtttctatg
    37981 gcaaaaccag tccaatttct cttcatcttt cggcaatagg ttattctaac atagtttttc
    38041 gttttctgca agtttacaga tttttatttc atttgctgaa gttgttctac cacttcaaat
    38101 aagcccatgc tgttgctgcc ttttagcata atactatctg ttgcttcaag attttgtttg
    38161 atagcatcaa tcaatttatt cttttctttt tcaaaataga acacaggtag ttgggggtat
    38221 ttttggttca attcttcttt tagtgcatgc atttgttccc cataaaggaa aatcatggcg
    38281 aatttctcag gatcgatatg ttcactcatt tgcgcatgca tatttaaaga ctctttccct
    38341 aattccagca tatctgctaa cacagcgatt ctgcgtcctt ttgttggcag atttttaaaa
    38401 gtatctaata ccagtcccat ggctgttgga tttgcattgt agacatcgct caaaatagct
    38461 gcaccatttc ctgccgttag ccattcggtt ctgttttgag tgatagaaac atgggcgagc
    38521 ccttggcgta tctcaggtat ggacagtcca aagaagcgcc cgatcgtata cgcaatcaaa
    38581 gcattcgtta cattataact tccaggcagc gggatcgtaa atgtttctcc ttctactaca
    38641 aaatcggttt gtgcttttcc ttttgctgtc acttccgcat gaacatcgcc atttccaata
    38701 ccgaatgttg tgactgtttg tactaatgtt gccgttaaag gtgtcagcaa cggttcatct
    38761 gcgggcacaa cgagtaatcc gtctgataca agtccgtcag tgatttccat tttggcttta
    38821 gcgattcctt ctcgcgaccc aagattttct atgtgtgctt caccgatcat tgtgattgct
    38881 gcagcttttg gttgagccat aagtgaaagt tccgtgattt cattggcgtg atccatgccc
    38941 atttccaaaa cgatcttttc tgtatcttgg ggcatgtgca aaatcgtgta aggaagacca
    39001 atattattgt tgtaatttcc ttgcgtctta tatgttttgt atttttcaga caagacagac
    39061 gcggttagat ctttcgtggt ggttttacca ttgcttcctg taattgcaac gacttctgcg
    39121 cctacttttt ccaagtaata ggttgccaat tgctgcatgg ctttcaaagg atctgctaca
    39181 ggaataactg gaaaattttt tggagcttgt tcaacaggca agctccaaaa tgaagcagat
    39241 gctccgtttg tgatcgcttg ctggataaat tcatgtccat ctcgcgtacc agcaagagga
    39301 acgaataaac ttccttcggt tatcttccgg ctgtcaaatt cgacattggt aatctttgtc
    39361 agtgcagagc attcgctgcc cacggcttgt gcaatttctt gtattgttag attcattgag
    39421 atctctccta aaaaaggttg tgaaaaaagc gtttggccta aacggcgggt tcgcatgact
    39481 ctcacttcca agtaaatttc tctattcgta tttagaagct agaatgaacg cggttacgca
    39541 atcactccag cctcttcttt atttctcttt attcattttt ttcaaaaaaa gattgacgct
    39601 gatggtatcg attcattcct aactggatca gttcttcaat caaatcaccg tatttcaagc
    39661 ccatattttc ccataagagt gggtacatac tgaactccgt aaatcctggc atagagttta
    39721 attcattcag gaataattca tttttatttg tcaaaaagaa atcgcaccgg ctcaatccgc
    39781 ttccacctaa catcgtgtaa gctaacttcg cgtactcttg cgctttttga taaacttctt
    39841 ctggcacttc ggctggaatc tgcatttcga ttttattatt gatatatttt gcttcataat
    39901 catagaatgc tacgtctttt acgacttcac caggcaaagt cgtccgaacg tcttcatttc
    39961 ctaatacagc aacttcgatt tcgcgcgctt caattccttg ttcaacgatt gctcgagaat
    40021 catactgata ggctgttgct aaagcatttt gcagctcttc tcggttttct gcttttgtaa
    40081 tgccgacact agaacccata ttcgccggtt tgacaaacat cggataaagc aaagaacctt
    40141 cacattgatc aaatactttt ttaggatttt ctttccattg attcttaagt actggtacat
    40201 aaggaacttg cggcacacca gcagcttgta aaatatactt ggtcatgatt ttatccatgg
    40261 cacatgcact ggtcaatacg cctgcgccga cataaggcat attcaatgtc tctaagaagc
    40321 cttggatcgt tccatcttcc ccgtttggcc catgtaaaac tggaaaaacg atggctcctt
    40381 cttctttgat ttcgcccgga ttgatcactt ttcctgtaaa gccttcctct gtctgtccac
    40441 ttgggtccca tgaaagatgc aagacatctt tgctggcagg tttttctgtt agtaatggac
    40501 ctttgaccca ttgtccttct tttgtaataa atacgagttg aacttggtaa taattataat
    40561 aaatggcatt taaaactgaa aatgcggaaa gaatggacac ttcatgctct gcgctgcgtc
    40621 cgccatatag taaagtaatc ttcaaaagat tgtcctcctg tattctaaat cgttcagtga
    40681 tttactctca tattttagca caaaacaaaa ataaacgaat aacttattta taaaaaaacg
    40741 agccagcttc taaaagaacg agcgtcattt attttttgtc agctagatta tttgtcccgt
    40801 tttttataaa aagttatgat aaagaaaagg agggatgatg atggcgcaaa caaatccacg
    40861 cagccgcttg gcagaaatca ttcgagtgtt catcaattac aatgtagtgc caaactttgt
    40921 acagcaaaaa aatcccgaac aggtaaaaaa agcctttgaa gaactaggcc ccaccttcat
    40981 caagatagga cagatgcttt ctgttcgaga agatttgctt tcttctgcct tcactcaaac
    41041 attcaaaaca ttacaagata gtgtacctag cgacacattt tcaacggtca aaaagacgat
    41101 cgaaacagaa ctttctcttt cattatcaga tatattcgat gatttttcaa aatcgccttt
    41161 tgcctctgct tctatggggc aggctcatcg tgccaagctg aagaacggtg acgcggtagt
    41221 cgttaagatc caacacccta acatcgccga agagatccga cttgatcttc aactatttga
    41281 acgagcgatc cctttgatca aatacattcc tgaaactagc gtagttgatc taaaaggggt
    41341 actccaagaa gtaaaacgtt ccttgatcaa tgaaatggac ttcttaaaag aaagtcaaaa
    41401 tggcgaacaa ttctatcaaa agaacaatgg ctggaaagag atccgcagcc ccaagattta
    41461 tgacgctttt tgttcgaaaa aagtgatcgt gatggaagaa atgtctggta aaaacttgaa
    41521 tcatttaatg aacgccgaaa acaaaactga aacttttatc actggaattc aaaacaagca
    41581 gctgaaacaa gaagttgcaa agcttttagt tgagaacttt atgaaacagg tgtttgatga
    41641 tggttttttc catgctgatc ctcatccagg gaatctgcta ttccatgtac taacaaaaga
    41701 agaacagaca caagctagcc gaaaaactga aacagtccat gaaaaagagt tcggttcctt
    41761 tgcttttcga gcctccactt ctgcagaaga ccctgttgcg ccatatacca tcaactacat
    41821 cgattttggt atgatggggc atctttctgc aggacttagg caaaaactca cacaagccgt
    41881 tttagcgctg tatacgaaag atgcttatcg aatcgaaaaa gcggtccttc gactttgtca
    41941 gcaagaaggc tcgtttgatg aaagtcggtt ccaccaagaa ctgacaagtt ttcttgaaca
    42001 gtattatgat tctcctattg atgaaatcaa cttgcaagaa gtattcacac atgtcgtgac
    42061 gatttgtcat caaaataatc tgcagttcga tcgtgatatc acgcttttgc tgaaagcatt
    42121 tgggacatta gaaggggtga tccgcgtcct cgatcctgaa gtttccctca tggaagtcgc
    42181 ttcgcctttt gctcaacatt attttctcac tcatctggat gtagaagata cattgaaaca
    42241 aagcggatta gacttattgg aagggatgaa ggctgcgcct aagatccctc aacagctcca
    42301 ccatctatta gaaatgtgga cttcgggaca aggaaaagtc aatttggaat tgaaaaagca
    42361 agacaaatta ttgagcagga tcgaaagtat gatcaatcgg cttgttttcg gtatgatttt
    42421 agctgcattg atcgtagggt cctccctgct tgtccaagca gcacctgtag aaaacgcaga
    42481 ggtcgtttcc ttattaggga tatttacgta tgctattgcc gcattcgtca ttattttttt
    42541 ggcaatcgat gcattgatcc aaatgtataa gaagaggaaa aagtagaaaa aggttgtgac
    42601 agaaagaatc agcttcaaga aataagaagg ggttttacat aaattgtttc tcaaattttt
    42661 gtgaaaattc ggcttatttt tcgaagaggc tacttctgaa acaccgagaa taaaaaatgt
    42721 gaaaaagctg ttctgcaccc agtaataaga acaatcaaaa agggaacatg gaacacattt
    42781 atcctgtttc cctgttccct ttttgatgta ttcgagcata aataaaatac cttcgttacc
    42841 tactatcttc tgtacttggt aaaccttagt caattgcctt ttttaacgct tgacgaaaag
    42901 acatatgatc tttctccgta aagggttttg gaccctttgt ccttatgcca gcttttctta
    42961 actttccact atgataacgt tgttctagaa gcgtgccgat attcgcttcg tcaaattctt
    43021 ttccagagtg atggaacact cgcacaccct tcggcaatac aagtgaagct aaaccataat
    43081 cttgcgtgat caaaaagtca ttcttcttaa ccataccgac gattttataa tccgcactat
    43141 cagctccgcg gtctacgtag acgatttgga catgattagc gtattctttg gtcgtataat
    43201 ggtccacact ggtcacgata accacgtcaa gaccatagga ttctcctaat tttatgacat
    43261 cttcttttac tggagaacca tctccatcaa tgaatagcct cattcttttt cgccttcttc
    43321 cataaatgat aaacagttac taatccgctc agtaaaagac caacattgat ccattttgcc
    43381 aagataagca gttgtacact ttctatcggc actgattcat ggttcagcgt gatcaataat
    43441 agatggattg gctctcccat ttcttttgct aaaacaaaca aaagcgtcat caataaaaac
    43501 tggtggatca cactcccttt attttgagta gtatacttga caacgatccg gcgatccatt
    43561 ggaatagctg tatcagtttg ataatattcc ttatttagcc agctgcctgt tactacgtgc
    43621 cagaaactaa gcagaaagac acctatcgca aaaccatttg gaatcaagag gaccaataaa
    43681 gaagaactca gtccaagcaa ttgcatagcc aatggagacc atgttaaacg tttcaaaatc
    43741 catggtccga ctatttttgc tgcaatcaag cctgctgcat aaggcaaata cataaaaatc
    43801 gaaagttttt ctaatccgta cagtatgcca acataaaaac tgctaaataa aaaaaggaaa
    43861 ttccctagca ttccatttag aaaagtaaac agatttagcc agcttggcaa cttccattcc
    43921 ttcttctggt gtcctaaaac aaacacgaac aagatgaaca gcagcaagaa accataaata
    43981 gcaagatcaa aaagcctttc atcgaataac aatcgggcat ttctcaaaag aaacaataag
    44041 ccaaagaata tcaaaaataa aacaagctct cgcgtagcta ccagatttct tttgatgtct
    44101 ttgaaatcca gttcgtaacg cggataatga ctaaccgtat ggtaggccat gacgtaaaaa
    44161 agtgtataaa tagtaaagct tgctgcaatc tgagtcggtg caggcaactc aatcgcttga
    44221 tacaggagaa atagccagat agctgcaaat aagtactttt tcccatccat attgataaaa
    44281 ccttgcgtct tttcgtgaaa attgaccgtg gtattagcag gtacaagcca tgctgcactc
    44341 agccctagca aaacacttga aaaataaaat aaaggaaaat aaaatacgcc taatattcca
    44401 agcaatgaac cagcaccacc aatcagcaga gcgatcatca gcaacgtgta actatctaga
    44461 ccaaatcgta gactgttgac taaaaatagt ccagtcatcc gaaaggcata aaacagcgta
    44521 aatggcaaaa tcgtcagcca attatgcgag tctgttagtc ccaaaaataa aagataagga
    44581 ataaaaataa ccgtgttact taagataaag ggcgttgttt gtgtaaaatt acgcccaaat
    44641 cgtttgagtt tttcgaacat gacaatggct ccttaaacag tcttataact aatctgctct
    44701 tcgattttag cattaaaggg gaaataaacc aattttacag aatatattct tgtaaaaaaa
    44761 aatacagaaa gaagcataag cttctttctg catttttacg gtacttgatt actcaagcag
    44821 acaagccctt ctccacaaaa gtcttgcccg acacccctgc atccggcagg tgataaagat
    44881 agaaatcaat tatcctgtgc ctccaaaatc aattaggagc tctgtaaacc agagtccttc
    44941 tcgacccgtc gcataaattt actttaacac agttcctatg tttatgcgag agcttttttc
    45001 attttataat cgaccatttg aaatccttga ccgatatctg aaataagttc ttctgtaatc
    45061 tcaaatcctt catgcttata aacctcgatt gcttgatgat tcccctgatt cactcgcaaa
    45121 aagatctctc tttcctttgt ttgcgcagca agttcttcat accagcgaaa aactgaagaa
    45181 gtcaatcctt gaccacgtac tttatttagc aaatataatt tacttaaata aatttttcca
    45241 tttatctctt cataagcagt gtaaccgaca gtcttttctt cgctttttaa taaaaagtag
    45301 gacacacctt ctgtcagttc tttttgaatc tgtttttttg actgataagt tttcagcatg
    45361 taatccactt gtttttgacc aatgatcggc acaaaaactt ccggccatac ttcttgtatg
    45421 actgccatca gctcatccag ttcattttct gttactttta ttttttccat acacccacgt
    45481 ccttttttac attataaatg accttgcgct tgatggaata aaaagagcat gatcatgcta
    45541 tacacaccca ccgaccacgg tttcaagaaa atcacaatct gcataaattc cctaaaagat
    45601 aatttcgtca aaccagctac tagacagatc aagtcgtctg gcgcaaaggg aagcagcaag
    45661 gtaacaacta gtactttttt aatatctttc gtttttcttg tcagcaattc atcaaatttc
    45721 tgatactttt ttggcgataa aataagctgg acaaaggcta cgccatagta acggacaagc
    45781 aaaaatccga ttgcttcccc aatcactagg ccaatacaac tatataaaag cccaaatcca
    45841 ttgccaaaca tcagcatgcc cgctactgag gagatccctc ccggcaaaat aggaatgatc
    45901 acttggatca cttgcaacag aacaaatatc atcactgcat acttgccaaa ttgctggata
    45961 aacgcttgca aagacgtgct cgatcgaaaa atcccatgat gataagcata aagaatcaac
    46021 acaaagaaaa aaaacagacc aataatgggc atgatctgaa gacatttttg taaaactgat
    46081 ccttttttag acatctatat tccccacatc ttttttcatt tattaaaact gcctcct
//
LOCUS       DACDXE010000016        42948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.016, whole genome shotgun sequence.
ACCESSION   DACDXE010000016 DACDXE010000000
VERSION     DACDXE010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.016"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            188..391
                     /locus_tag="IWM23_000892"
     CDS             188..391
                     /locus_tag="IWM23_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883555.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            602..1186
                     /locus_tag="IWM23_000893"
     CDS             602..1186
                     /locus_tag="IWM23_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9883556.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            1557..2882
                     /locus_tag="IWM23_000894"
     CDS             1557..2882
                     /locus_tag="IWM23_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9883557.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQINEEN
                     LDKIIDKIRQKYGFTSIVHASSMLESARSITRSTLVGGHAGGNGGIKND"
     gene            2875..3225
                     /locus_tag="IWM23_000895"
     CDS             2875..3225
                     /locus_tag="IWM23_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883558.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIVGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            3222..3401
                     /locus_tag="IWM23_000896"
     CDS             3222..3401
                     /locus_tag="IWM23_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883559.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            3537..3827
                     /locus_tag="IWM23_000897"
     CDS             3537..3827
                     /locus_tag="IWM23_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9883560.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            4195..4983
                     /locus_tag="IWM23_000898"
     CDS             4195..4983
                     /locus_tag="IWM23_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9883561.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTTAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            4970..5296
                     /locus_tag="IWM23_000899"
     CDS             4970..5296
                     /locus_tag="IWM23_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883562.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNYKPKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            5645..6685
                     /locus_tag="IWM23_000900"
     CDS             5645..6685
                     /locus_tag="IWM23_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAP9883563.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDSTVIAKIANTAKPRKINDST
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMQEDSINTFVPENVLNLIKTFSSSYSEAQQTVQTIHNAKKKAEELEQTIVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            7521..7853
                     /locus_tag="IWM23_000901"
     CDS             7521..7853
                     /locus_tag="IWM23_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9883564.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            8444..8773
                     /locus_tag="IWM23_000902"
     CDS             8444..8773
                     /locus_tag="IWM23_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883565.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            8760..9101
                     /locus_tag="IWM23_000903"
     CDS             8760..9101
                     /locus_tag="IWM23_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9883566.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDQGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            9215..9454
                     /locus_tag="IWM23_000904"
     CDS             9215..9454
                     /locus_tag="IWM23_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883567.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            9521..9760
                     /locus_tag="IWM23_000905"
     CDS             9521..9760
                     /locus_tag="IWM23_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883568.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            9846..10517
                     /locus_tag="IWM23_000906"
     CDS             9846..10517
                     /locus_tag="IWM23_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9883569.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            10570..12546
                     /locus_tag="IWM23_000907"
     CDS             10570..12546
                     /locus_tag="IWM23_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9883570.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGAAQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            12559..13215
                     /locus_tag="IWM23_000908"
     CDS             12559..13215
                     /locus_tag="IWM23_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9883571.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPAVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSIPLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKK
                     "
     gene            13311..14138
                     /locus_tag="IWM23_000909"
     CDS             13311..14138
                     /locus_tag="IWM23_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883572.1"
                     /translation="MNKRKWLFVCVAALFATAAYSQISFADSSTTPTTSQTTDSSTTA
                     TSDTDSSKNTAEETADSSTSSTESAESTSESSSETSEKLQEVEDTQVIKQNIHIQKII
                     SSNRINNQGRQVTQQEGVNGAKFIVYDITNILEEMTQDDNEKNQSVEIIESKLKDRAK
                     KLSYDQLKKVTEGETQTIDDQEGVIDFSIEVPANKKQAYYIVNESSPENISNSEDIVL
                     LTPVSDEKGEFLTDVWLYPKSKKSEPKEEIKKVVSTGVKKNFFDNCWDFFTHLFFND"
     gene            14148..14396
                     /locus_tag="IWM23_000910"
     CDS             14148..14396
                     /locus_tag="IWM23_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883573.1"
                     /translation="MKIIKMLVFNLSFLAATILCCGLVFSQLSIYPRYQRLFVHFLNI
                     QHASMPLEVINKLLINPIFMASLFLLAGLVTWVLGKKE"
     gene            14427..16538
                     /locus_tag="IWM23_000911"
     CDS             14427..16538
                     /locus_tag="IWM23_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9883574.1"
                     /translation="MEGIMENKIHCTTGRVFTLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNVKLDFKYAGSKTKVALSEPQPEINSKMRSMRAAGIELNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMDRLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNKVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQD
                     PVEARLTYKEGSARVIDMSTGKDITSEGILTYDSHSRTLKWEASADFLNNNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQVIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(16576..18633)
                     /locus_tag="IWM23_000912"
     CDS             complement(16576..18633)
                     /locus_tag="IWM23_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAP9883575.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYMVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVDILKEQIINVTHGESVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFSTPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(18747..18959)
                     /locus_tag="IWM23_000913"
     CDS             complement(18747..18959)
                     /locus_tag="IWM23_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883576.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            19146..19613
                     /locus_tag="IWM23_000914"
     CDS             19146..19613
                     /locus_tag="IWM23_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883577.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            19610..19852
                     /locus_tag="IWM23_000915"
     CDS             19610..19852
                     /locus_tag="IWM23_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883578.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            19870..20172
                     /locus_tag="IWM23_000916"
     CDS             19870..20172
                     /locus_tag="IWM23_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883579.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            20243..22297
                     /locus_tag="IWM23_000917"
     CDS             20243..22297
                     /locus_tag="IWM23_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883580.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            22297..24129
                     /locus_tag="IWM23_000918"
     CDS             22297..24129
                     /locus_tag="IWM23_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9883581.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFSEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            24824..26740
                     /gene="ltrA"
                     /locus_tag="IWM23_000919"
     CDS             24824..26740
                     /gene="ltrA"
                     /locus_tag="IWM23_000919"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9883582.1"
                     /translation="MERDKTMTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSN
                     FKNLMKFIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKD
                     YRPRKVKRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNC
                     STENAIADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRK
                     MLKAPIVMRNGNVVFPTKGTPQGGILSPLLANICLNEFDWWIANQWEERTVKELKHHY
                     RKNGAWDKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERL
                     KLPISMEKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIK
                     KIQYAGAKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARG
                     MTKNGIYGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRK
                     LIHQEQQAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEI
                     ECHHINPYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTY
                     RKKAANFPISIKMN"
     gene            26857..27657
                     /locus_tag="IWM23_000920"
                     /pseudo
     CDS             26857..27657
                     /locus_tag="IWM23_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            27654..30185
                     /locus_tag="IWM23_000921"
     CDS             27654..30185
                     /locus_tag="IWM23_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883583.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYISSPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEDKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            30235..30567
                     /locus_tag="IWM23_000922"
     CDS             30235..30567
                     /locus_tag="IWM23_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883584.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            30568..31161
                     /locus_tag="IWM23_000923"
     CDS             30568..31161
                     /locus_tag="IWM23_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883585.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            31324..33018
                     /locus_tag="IWM23_000924"
     CDS             31324..33018
                     /locus_tag="IWM23_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAP9883586.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKCFLAMIDECHNGATCSAVKSCGLQVV"
     gene            33706..35583
                     /gene="ltrA"
                     /locus_tag="IWM23_000925"
     CDS             33706..35583
                     /gene="ltrA"
                     /locus_tag="IWM23_000925"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9883587.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            35714..36091
                     /locus_tag="IWM23_000926"
                     /pseudo
     CDS             35714..36091
                     /locus_tag="IWM23_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            36091..37296
                     /locus_tag="IWM23_000927"
     CDS             36091..37296
                     /locus_tag="IWM23_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9883588.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPSLNSSKK"
     gene            37312..37956
                     /locus_tag="IWM23_000928"
     CDS             37312..37956
                     /locus_tag="IWM23_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883589.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            37967..38773
                     /locus_tag="IWM23_000929"
     CDS             37967..38773
                     /locus_tag="IWM23_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883590.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            38795..39025
                     /locus_tag="IWM23_000930"
     CDS             38795..39025
                     /locus_tag="IWM23_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883591.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            39571..39846
                     /locus_tag="IWM23_000931"
     CDS             39571..39846
                     /locus_tag="IWM23_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883592.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            39888..40310
                     /locus_tag="IWM23_000932"
     CDS             39888..40310
                     /locus_tag="IWM23_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883593.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            40329..41921
                     /locus_tag="IWM23_000933"
     CDS             40329..41921
                     /locus_tag="IWM23_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883594.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            41933..42268
                     /locus_tag="IWM23_000934"
     CDS             41933..42268
                     /locus_tag="IWM23_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883595.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            42415..42609
                     /locus_tag="IWM23_000935"
     CDS             42415..42609
                     /locus_tag="IWM23_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883596.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            42641..>42948
                     /locus_tag="IWM23_000936"
     CDS             42641..>42948
                     /locus_tag="IWM23_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883597.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     T"
BASE COUNT        15832 a         6567 c         8600 g        11949 t
ORIGIN      
        1 agtgacagga acgatgattt ttgataaagt tggttggcct tctagacctc ctcaatcacg
       61 gaattggaaa gaaaaaagaa atggttattg gtactatgga aaattgtatt tacaaagtta
      121 taaatcagct ggaaacaatt ggattgcgac ttattcagga accttaacta gaaagtaggg
      181 attttttatg ccagaatttg tgaacacacc aaatttttat caagatagcg atattctaag
      241 taaaattaat ttaccgaaag aagaatcagc tttacaagtt cagcgaactt tacaggagac
      301 acaaaaaatt caaaaatcaa tttctaatct agaagattct gaagaaaaaa gaaaaaaaag
      361 agaccaaaaa acatatcaaa aagaaaaata atgattttaa aaaaagagtg tctagtcgac
      421 attctttttt tctaattgaa agtgttcata aaatcaccca tttctggaca cttccttttt
      481 ttgctttatt tttcaaaaaa acaattcatt tcatgttcat aaacccataa caatatctat
      541 gttcagttaa actatatgga taaagttatg ttacaataaa attaatgaag gagtgatttt
      601 tttgaaaata ggatacgccc gggtatctac tggtttacaa aatttggatt tacaagaaga
      661 cagcctaaat aaatttggtt gtgaaaaaat ttttactgat catatgagtg gggcaaaaag
      721 taatcgacca ggattagaaa tggccattga ttttgttcgc tctggcgata cacttgtcgt
      781 ttggcgctta gatcgtttag gtagaaatat ggaagatttg atttcaatcg tcaatcgact
      841 gaatgagcgt ggtgtgagtt ttcatagtat tcaagaaaat attacgatgg acaaatcaag
      901 ttctacagga cagctcatgt ttcatttgtt tgcggcattt gcagaatttg agcgtaattt
      961 gatcctggaa cgatccgcag caggacgagc agctgctcgt gcaagaggac ggtttggtgg
     1021 tcgtcctgaa aaattgtctg aacaagattt ggaattactg aagactttag tggatagcgg
     1081 tacacctatt aagactattg cggaacggtg gaacgtctca agaaccacca tttatcgtta
     1141 tttggcaaaa atggaggaga aaaatggaaa gacaaattct cattagtttt tgtcatttaa
     1201 tgctgaaaat ttttgccatt tatctagaaa agtgacaata gtatgagaat tttttgtaag
     1261 tgtctactga taacttatta tcaaatgttg taaggatttt attcatttgt taatattaaa
     1321 gaacggaact ccaaaaaagt ccgtactcta atttgcagtc tctcactctt ttagagtgag
     1381 agtttttatt agattttcaa attaattagt aaagtgatag aataaattga tcatacaaat
     1441 cataagaaga agcagggtga tagcatacct atgcttcttt ttttatgtat ttagctattt
     1501 cttctttaca acatcgaaca tatgtttgta taatgtggaa gaatggagtg ttatttatga
     1561 tggattattc aaaagagcca gtaaatgatt actttttaat cgatatgaaa agcttttacg
     1621 caagtgtcga atgtatagaa agaaatttag atccattaac aacagaactt gttgttatga
     1681 gtcgatctga taatactggt tcaggattga tattagcttc ttctcctgaa gcaaaaaagc
     1741 ggtatggtat tacaaatgtg agtagaccac gtgacttacc acaaccattt cctaaaacac
     1801 tacacgttgt tccaccacgt atgaacctat atatcaagcg aaatatgcag gtaaataata
     1861 ttttcagaag atatgtggct gatgaagatc tactgattta ctcgatcgat gaatcgattt
     1921 taaaagtgac aaaatcactg aatcttttta cgactgaaga aacacgaagt cagcgaagga
     1981 agaagctcgc tcaaatgatc caagaacgta ttaaagaaga gttaggtttg attgctacgg
     2041 taggcgtcgg cgataatccc cttttggcta aattagcctt agacaatgaa gcaaagcata
     2101 atgaaggatt tattgcggaa tggacgtacg aaaacgttcc tgagaaagta tggaatattc
     2161 ccgaaatgac ggacttttgg ggaattggtt ctcgaatgaa aaaaaggctc aaccaaatgg
     2221 gaattttgag cattagagac cttgctaact ggaatcctta tacgattaag aatagattag
     2281 gtgtgattgg attacagctg tacttccatg ctaacggaat cgatcgaaca gatatagcaa
     2341 tcccgccaga accgacaaag gaaaaatctt atggtaattc acaagtgtta ccacgagatt
     2401 atactagaag aaatgaaatc gagctggtgg taaaagaaat ggcggaacaa gtagccattc
     2461 gtatcaggca gcataattgt aaaacaggat gtgttcattt aaacattggt acgtcgatct
     2521 tagaaacgag accaggattt tcacaccaga tgaaaatacc tatcacagat aatacaaagg
     2581 aattgcagaa ttattgtctt ttcttgtttg ataagtatta tgaaggacaa gaagtaaggc
     2641 acgttggaat aacctatagc aagcttgtgt acaccgattc gcttcagctg gatttattta
     2701 gtgatccaca aaagcaaatt aacgaagaaa atttagataa aattattgac aagattcggc
     2761 aaaaatatgg ttttacgtcg attgtacatg cttcttcaat gttagaaagc gctagaagca
     2821 ttactagaag cacacttgtt ggtggtcatg cgggcggaaa tgggggaatt aaaaatgatt
     2881 gattacaatg atcgtggacg tttaaagtgg gcagggtttt atttatctga tcatactgaa
     2941 gaaattgatg ccgacaatga acaacgaacc agtcaaaatt tagcaaaaga acaaatgacg
     3001 actgaagaga ttgcggaagt attgaacgct gcagtcataa aaaatagatc agtatctatt
     3061 caaaaagaag aacgaaacgc tgaaggatac tatccagaag atattgttgg caagatctta
     3121 ggtagtgatg agctggggat ctacgttgga aaagacaaaa ttcactacga cgaaatacgt
     3181 catatcgaat tttaccagga taataaatgg agtcgattag aatgaacatt atccagcaat
     3241 atgagctaaa gtatataacc tttgatcagc tatcagaaga gatttggggt tatggccaac
     3301 gtttgataaa tgaggttggt gttgagcgct tctctttcta tgtagaagct gcagcaggtt
     3361 atcataattt taggttttat attttcccgt tatatatata aggtatttta cggattctat
     3421 agagttatct ctatgaatcc ttttttgttt ttacaaatag gagtagatac tagaataatg
     3481 cgtgtgttaa actattattg caaacgcaat aataaaagaa agtaggctta cgcaccatgt
     3541 ctatcaatac atttgtttac tcgcacccga tcaatgttta cattatcaag aatttaggaa
     3601 tcactgtaga acaattttgt gagttatatg cctacccaca aggaacggtt gcaagctgga
     3661 taacccgaca acgtagaata aaatcattac ctgcttcatt cgtttatgat ttaagcctgg
     3721 cctctagttt aaacatgtct gatgtttatg aaaagctcct gtctttagaa aaagaatatg
     3781 attccttcaa gattaaacat gataaaaaaa taaaaaaaca tatataatat caatcacaat
     3841 tgtataatat tacgatatct taatatcatg atatcgtaat attgtttttt tctaagaaaa
     3901 tgcactataa gaagggaatt attagtaaca atatagacag tttaataatg tttaaattat
     3961 tctagttgct gctcgcaaaa catatttctt atgattaata aaaaatttta aagtaataaa
     4021 aagtgattat tttactttcg aataacatat gaataatctt tatatccgaa ccgatatata
     4081 tctttaaaac aaaataatca cacaatatta caatataatg catatacgat attgcaatat
     4141 cacaatatta tgatataatt atattataat ataagatgaa tgggggatat tttaatggca
     4201 agaatattgt cagtagccaa tcaaaaaggt ggagtttcta aaactacgac tagcgttaat
     4261 ttagcagcat gtttgaaatt tgtttacggt aaaaatgtac ttttagtgga cattgatcct
     4321 cagggaaatg ctacagataa tgtaggtttc gatatagatg gaacaaacca acctacaatt
     4381 tatgaagtat taaaagggga ggctgacatt acagatgcta ttctggacta taaaggaatt
     4441 gatgttttgc cagccgacat agccctttct tctgcagaga gggaatttac tcaagtagga
     4501 agtgaacaca ggcttaaaag agtacttcaa ccaatcaagg acaactatga ttatattata
     4561 attgattgtc caccaagttt aggaatgctt acagtcaacg catttactgt ttctgatgaa
     4621 attatcattc cagttgaagc tgcgtatttc tcattaaaag gactaataaa gttaagtgaa
     4681 actattgaaa cggtaaaaga atatacgaac gacaagcttg aagtacgagg agtcctattt
     4741 actaagtata ataagcgtta taacatttct aaagaaatga cgactgcagc aaccgaaatt
     4801 tctagagtca ttggtgcaaa aattttcaaa acttatattc gtagaacaat aaccgttgac
     4861 gaagcgcaag ctgctggatc agatctaatc aattttaata aaccctcaac agctgaagat
     4921 gactataaag catttacaga agaatattta aaagaagttg gtgattttga tggcagaaga
     4981 taatcgcagt caagctgcag taagtagact actaggaaat aaaaataaaa agaactacaa
     5041 accaaaagaa gtagaagaaa tcgaattaac caagaaaaat ataagtaaag tcataaaaga
     5101 tcaaggaagt agtgtaagaa cagcagatag cattcgtata aatccaattc taatgtcagc
     5161 actcaaatat tggacgacta ttgctgaatc agacaaaagt aaaccagacg ttatagaaga
     5221 tgcgctatta aaatccatac ctgaagaata cttgattgaa ggatataaac tagcaaagaa
     5281 acaaaataaa atttaattta cattcaatat cacgatatca taatatcgtg atattttttt
     5341 tataaaaatc agtagtatca aagctgataa gtcgcttatc ttcatattgt aatattacga
     5401 tatcgtgata taatgttatt gtaatatcgt gatatagtta ccatgagctt aaaccctgaa
     5461 atagagcggt ttgctttaat ttatttttta aggtaaacta aaaaaagata aaaaaagact
     5521 actgctttaa caaagtttgg cgacttatca gcagtagaaa aatatatagt ggatagatat
     5581 atttgtttaa ttcattgtaa catagttttg aacaaaacgc tatcctacag aaagaggatt
     5641 ggctatgaat gattttaatt attacaaatc aaaagaaatt tatcgtgaaa aatattatca
     5701 aatgcctaag gtatttttta ctaacgaaaa atacatggat ctttcaaatg atgcaaaaat
     5761 tgcttatatg ctacttaaag atcgatttga ttattcagtc aaaaataact gggtagattc
     5821 tgatgataat atattcttca tattcacagt tgaagaatta atgaaattgt tacaatgccg
     5881 cgaaggtaaa gtatctaaga tcaaaaaaga attagaaaaa gcaggtcttc tgaaacagaa
     5941 gagaggaaga gtaaataaac gtgatggtaa gattgaatct atgcctaatt tattgtactt
     6001 aggcaagcca gaagttacga atcaagacgt ctttaaaatc atggaagagg aagacaatac
     6061 tgattccacc gtaattgcga aaatcgcaaa tacggcaaaa ccgcgaaaaa tcaatgattc
     6121 taccgtaatt gcgaaaatcg caaatacggc aaaatccagt catatcaagg actcaaccgt
     6181 aattgcgaaa atcgcagaca atctattcta ttctaattct atagacacta atagacacat
     6241 aatagacact gaaactgacc agttacaaaa tcaagttttg ctagataact tcgtcgacat
     6301 catgcaagag gacagtataa atacatttgt accagaaaat gtattaaact tgataaaaac
     6361 attttcttca agttacagtg aagctcaaca aaccgtccaa actattcata atgcaaagaa
     6421 aaaagctgaa gaattagagc aaactattgt cgtttatgaa gaactggaat cttatggaat
     6481 tgatgctgac aaaggattat atatgacttt actgaaagct tatcaaaaac aaaaaactga
     6541 aaaagtaaac aatttgcaga atctaatttt cgtttatgta aaaaattggt ttgtcgaaaa
     6601 agctattgcg gcaaagcttg caaacgaaca aagagaaaca tttaacgaat taccaacagt
     6661 tagtacggaa aattggttag agtagttgat aaaaagcaat tactataaaa atgatcaatt
     6721 ttaaattaga acttttttat gattttaatt ttattacaaa tattacaaat aaatagttag
     6781 tattccggag tttttagatt aaaagtttat gaaaacaatc acaatcgtag gttttaaatt
     6841 cataatcgtt agatatttta gttatttttc actcaaaaaa atagggaact agtgtttgca
     6901 tatgtgcttt tttgagttat aataattcat gaagagaggt aattcactta tttccccaag
     6961 ctgaaaagcc tgatattact tcttttcagg cagtgctgct aatattcaat tacacttttt
     7021 tgtatatttt tagttgtttt aaatttaatt gtctgctatg cttgtgatga attgattttc
     7081 gttctgcact tttggacgat aaatttggac aattgaaaat acaaaaaaaa gaaaggccct
     7141 gtgagactcg gaaggctggt aaccttcgtc gacgtccaca ggtggcatta agatcactcc
     7201 cttaatgcgt ttacctttca gtgttacggt attacccgta acggatttag tataacagat
     7261 attcaaaagt gataccactt tttttggctg tgttaaggtt aaaaatacgt gatattacga
     7321 tgaatctatg cctaagcata atgttataaa aatgtccgtg tggctctttg ccgtatgacg
     7381 aacgctgaaa ttagagggaa cgcatgtttg gatgcgttcc ctcttttttg tttgtcatga
     7441 acgaaaatca attctctatt tttgaaaaat aacaagaaaa acttgttaaa aatatttaaa
     7501 gtaagtgggt ggattatttt atgtcatata caggaatttt gagtctcgaa gatatttgtc
     7561 actacggtaa acggtgtacc gctacagaaa agatcacgaa gaaactatca actggtcaaa
     7621 gtaaaaccgt tgtgcagtgt aagaaatata taatccagaa agacaaagtc tcagaagaaa
     7681 tgatttatta cattgggaaa caaaaacaaa taattttaaa agatcctatc cctttaaaag
     7741 agctatatcc aacaatcaaa catgtttatg accaaaacgg tgtactgatt ggtcgaagaa
     7801 aaaatggagt cttgcgttgt accgcaaaag ggatggggcg tttaatcagc tgaattgatc
     7861 attctcataa cttgtatttt atcgtttttc gcttgcttga aaatattgtc gagacttcta
     7921 aaggaactta gaatcactag aaaaataagg agtagacttg atctatcaac aattcctttc
     7981 ggggggtgac ggggtaggtt tttacgattg gtaacgtaaa aaattggtca atcgagtaaa
     8041 tgcccttgtt tggatagtgg tgaaatgaat ttgtgtaaat aatatgcaca gagtaaacaa
     8101 gagggaattt agaagttaga caagttagaa tagcgcctag attaatcatg agcagctact
     8161 aagggatcta ggctttgacg caaactcatg gaataaggag taggtcttct cgtaatgggg
     8221 aggacgcaca agaaattgtg tcgtgtgctg cagtacatgt agatacttgc gaagacgttg
     8281 atctttggat caatcgagca tttaccgttt tctactaggg tttctcgttt cggacgccat
     8341 aaacgagtcg atttatttta tatatttttg agtgaacgaa aaacgataaa aatattcgtt
     8401 atgaagtaaa actaatttag tagatagcaa aggtggtaga ggaatgattc aaaaatttgc
     8461 gaaaatgagt aaaactgcat ggattgaaaa gcttgaaaat gacttgagtg tcatgcgatt
     8521 agctccaaag aaattcaaac gagagattgc agaaaaagaa gcaattttgt cggtattgaa
     8581 tgcggtagac gataaatgtc cagtaaaaat tagtttttca gatatagcaa caattatcga
     8641 aggtatttat tatggtgaag ttgatgaaga gatatcttct acaaaagatt tattagattt
     8701 tttggataaa aacaaagata cctttatagt actaactgat tatgaggagg ttacaacaaa
     8761 tgaaaaatta tgatccgaat agccgatggg gaacacatac gattaaggtt tctttccaac
     8821 gttgggatta caaaggattt gtgacgttta gaagaggcgg aaattgtaaa ggattagatg
     8881 ttttagcatt agacgaggat gatttatacg atcaaaaatt gactgataat ccaattggat
     8941 tcggcttgct acctgaagat gatcaaggta atgaatggtt taaaatgacg cttatgaatg
     9001 ataatggaga cgaattatct gttgaagata cttggtcata tttgagtgat tatattgtca
     9061 gcgttgaaat cattgaattt gtagcggata aagaagaata aaaactggag gaataaattt
     9121 tacagataaa ggcttagaag gagtcacgac gattaataaa aaggtagtat taccttaggc
     9181 attgagtaag gaatttattt aggaggaaaa ttagatggcg cgaccagttt tagaggcgat
     9241 ggatatggat actttgacaa aaggaaggta tgtacagctg ttagctcgtg gttattcacc
     9301 taaagaagta ttcaaatccc tgtctaaagg aacagatatg gaaaaagaaa gattacgaaa
     9361 agaattcgat tattggagaa cccataatgg aatcaaggat ttgaaaccag cacggccagt
     9421 aattaaacga aagaaaaaga taacccaaga ctgaagtgta gagcgtatgt gatcataggc
     9481 ttttttattt ttgcaaaaaa caaggaaggt aggaaaactc atgaattatg aagaaatcga
     9541 aaacagaaaa aaagtgtcga aagaaatgga agaaaagttg ctaaaaacga tgaaacaaaa
     9601 acaccttaaa agattatctg tcatgcaata tatcaatgat atgcagataa caggaaaaga
     9661 gaaagcttgt ttgttaggtt caatgaaaaa ctttgaacag ttgagacgaa cttatgtgaa
     9721 aaaaagttct aattgtcaac tattgttaga agtctcatga cattcattaa tagaattcat
     9781 ttttaaaact ttttctatgg aaatgagaaa aagatttttt gttttgtctg aaagggggta
     9841 gctggatgag aaaatggtta tttggttttt taggagtcgc tttaatcgtc ttttgcagtg
     9901 tgtttggcta tgttagctac caaaaacatg aaggaaaggt gtttaagcaa aatatagaaa
     9961 aaaagatgcc cgtagatcaa atcaatgaac atgcaaagag ttataaggag gatgcaacga
    10021 acgtcaataa tgatatgtct ttggggcaaa tgttgtccat acaaaaagaa gcaatcgaaa
    10081 tgggtgtcaa taaacaagcc tttgcacaaa ttcagatacc tgcacttggt ttagcattgc
    10141 caatttttaa aggggcaaat caatacactt tgagtcttgg agcggctact tatttttatg
    10201 aagatgccga aatgggcaaa ggaaactatg tcctagcagg tcataacatg gaaatgccag
    10261 gtgttttatt ttctgatatt caaaagttgt cattagggga ggtgatggac ttagttagta
    10321 acgatggcgt ctatcgttac aaggtaacgc gtaaatttat cgtacctgag tactttaaat
    10381 taatcgatgg cgttcctgaa gagaactctt tcttgtcttt accaaagaaa ggagagaaac
    10441 ccttattaac gttgtttact tgtgtttata cgtcacaagg aaaagaaaga tacgtggtac
    10501 agggagagct acaataacaa ggttttaatc aacgcgagtc gttgaaatat gaaatgagga
    10561 gagtaaaaaa tggcaataaa tcggtatgta aaaaaagctg gaatgcttgt tggaatgtta
    10621 ggaatcattg caagctgctt tcaggggtat caagtacacg cagaagatgt cacgcaaaaa
    10681 acaccaccag agaaggtcaa tatcacggtt cataaattaa tgtacgatca aggaacgcaa
    10741 ttgaatgttg atattgacgg gatcaaaaac gatggttata cgcacgatca atatcctaca
    10801 ggtgtaacga aatacaacaa ggcagattat ggtgatgttg agtttacgtt aggaaatatt
    10861 acggatcaag tattaccgag agaagattct gatctaacca acgcgaaagt ggacgagatt
    10921 gtacaagatg tagaagacaa gggatctaac agtgagtacg tcaagaatgc aaccaacaaa
    10981 attacttcag ctgtagatga aaatggtgag atcactttta ctgatcaacc agcctatgtc
    11041 aattccaaag ggaatgtata tgtggtttat gaaagtaaat ccgctgctgg attagtcaca
    11101 caaaaagcaa aaccaatggt tgtgattgcg ccaatgacag ataaaacagg atcaggattt
    11161 ttaaaaaata ttcatttgta tcctaagaac attgtttcta agttgagttt tgaattaacg
    11221 aaatttggtg acgatggcgc agcccaaagc aaacaaaccc ttttaaaagg ggcaaaattt
    11281 gagttataca agggcgaacc tggaaaagga acaaaattgg gtgatcttgt atcggatgat
    11341 caaggaaagc tgactgcgac tgatttgact ttaggaaaat actattttgt ggaagttcct
    11401 tcggaagttg tagtgggatc agatcaagaa ccaacagcag atcaatattt attaggcgca
    11461 gacgctcgca acgatgcgca taataaatta acctttgaaa ttaccaatga cggcgtgaca
    11521 agtgacttaa aagcttccta cgtgaactat aaagcaccag tgatcgataa aacagtgaca
    11581 aatggaacag gacaagaaca ttctttccaa attggtgacg cagtgaatta tcaaggaacg
    11641 attcatatcc ctacagatat tgcaggtgga acagatggga ttacagtaaa tggtgtgaag
    11701 agtgaaactt ctccttacag cgtatttaag tggggagata cagcaggaca aggactttct
    11761 tatgtcgctg ctaaagcaaa tatcaaagta acaaataaag atggatctgt ggtattaaaa
    11821 gaaaatacag attataaaat ccaaaattca gaaaatggct ttgtgatcga ttttatcgtg
    11881 aataatggcc aagtaagtga tactgtagca agtttacacg gacaagatct tcaaatgact
    11941 tataacatgt atgtaaatga cagcgcagct gttgccaatc cattaacaaa tagcgtggat
    12001 ttcgtttata acaacaatcc atttaatcag gaagaacacc acgaaaaaac gaaagctgac
    12061 gttgtgactt atggtgcaaa attcttaaaa gtcgatagcg gactatttgg aacaggaatc
    12121 aaagcaacac cgcttgaagg cgcagaattt gctgctaaaa acgcagaagg aaaatattat
    12181 ggtggattag ttgatacgga taaagacggt gtcaaagaag cagtatgggt agacgatgta
    12241 gcaaatgcag cgatcctaaa atcagataaa gaaggtcact ttgaaatcac tggtttaact
    12301 gaaggtgaat attcactgga agaaacaaaa gcaccaactg gttaccaaaa attaacagaa
    12361 gatattgcat ttaaagtaaa caaaaactca tttaaagagg aaaatcgaat cactgttaaa
    12421 aataatcaaa aagcagctat tccattaaca ggtagtaatg gtttccaaac gtatgtattg
    12481 atctcttgct tgttgctagg agcaggggca ctttctgcag ttgtttactt taagaagaaa
    12541 gcctagaagg atcagctaat gattattcgt aagcccccaa aaagaagagt catgcacaaa
    12601 ctgatgatat ttttttggtt gctgtcggca gtagcagtct atttaattcc agctgtggaa
    12661 acaaatgtag cgaaatttca acaacagcaa agaattttag ccattgaaaa tcttgaaaaa
    12721 acagattctt ctttggatgt tgcgaaaata gagttaggcg atcctgttgg tgttcttaca
    12781 attccaagta ttcctttaaa actaccaatt tatgacggaa cttccgacaa aattttagag
    12841 aatggtgtag ggattacgga aggaactggc gatattacag gaggaaacgg taaaaatcct
    12901 ttaatcgcgg ggcacagtgg actttataaa gataatcttt tcgatgattt gccttcagta
    12961 aaaaaagggc aaaaattctt tattaaggta aatgaagaac aacatgccta tcaaatttat
    13021 cgaattgaag aagttaaagc agaagaatta caaaaaaatt atttagatta tctcatgcct
    13081 gaaaaagacc aagataggat cacgttaatg acatgcacgc caaaaggtgt taactcacac
    13141 cgctttttag tgtatggtga acgagtgtca ttcagtaaag aagatttaaa actacagaaa
    13201 aaagcaaaaa aataggaaat caatttcatt ggattggcga ttagcactgc tattcgcttt
    13261 ttttgcgttc ctattatttt tatttataaa aaaataagga ggagatcgga atgaataaga
    13321 gaaaatggct gtttgtttgt gtagcagctc tctttgcaac tgcagcttac tcacagatat
    13381 cttttgctga tagcagtact actccaacta ccagtcagac aacagatagt tcaacaactg
    13441 caacaagtga cacggacagt tcgaagaaca ctgcagaaga aacggcagat tcaagtacga
    13501 gttctactga atcagcggaa tcaacgtcag aaagttcttc agagacttca gaaaaactgc
    13561 aagaagtaga agatacgcaa gtaatcaagc aaaacattca tattcaaaaa attatttctt
    13621 cgaatcgaat caacaatcaa ggaaggcaag tgactcagca agaaggtgtc aatggtgcaa
    13681 agtttattgt ctacgatatc acgaatatct tagaagaaat gacacaagat gataatgaga
    13741 aaaatcaatc ggtagaaata attgagtcaa aattgaaaga tcgagcaaaa aaactttcat
    13801 acgatcagtt gaaaaaagtg actgaaggag aaacccaaac aattgatgat caggaaggcg
    13861 tgattgattt ttcaattgaa gtacctgcaa acaaaaaaca agcatactat atcgtcaatg
    13921 aatcaagccc tgaaaacatt tcaaatagcg aagatattgt tttattgaca cctgtttcag
    13981 atgaaaaggg tgaatttttg acggatgttt ggttatatcc taaatcaaag aaatcagagc
    14041 cgaaagaaga aattaaaaaa gttgtatcaa ctggtgtaaa gaaaaacttt tttgataatt
    14101 gttgggattt tttcactcat ttatttttta acgattagga tgaaatgatg aaaataataa
    14161 aaatgttagt ttttaatcta tcttttttag ccgcaacaat attgtgttgc ggtttagttt
    14221 tttctcaatt atcaatttac ccaagatatc aacgattgtt cgttcatttt ctaaatatcc
    14281 agcatgcttc catgccacta gaagtgatta acaagctctt aatcaatcca atttttatgg
    14341 ctagtttgtt tcttcttgca ggattggtaa cgtgggtatt aggaaaaaaa gaatgagaag
    14401 cttacaatcg tttaacaaaa taatagatgg agggcattat ggaaaataaa attcattgca
    14461 ctacgggaag agtatttact ttagtgttgt ttttgttcct tcttcttttt gcaaggggaa
    14521 ctgcagtcca ggccttagaa aaagttgatc cgagtcaaat caaagaaaat aataatctgg
    14581 tactttatca tgatagtgat tcattgagaa atatcgaagt aactgggatc aatggattta
    14641 agatactaga tgagtattca actaaaaaac aaaaagtact tgcttggcca aaagtcaaag
    14701 atttgaaaag cacgtcaaaa attgttgccc attatcaaaa ggtgggcact tatctaggaa
    14761 aagaaattga ttgcgacgta actttttcta attttgtagc gcacgctggg gttaaccaag
    14821 acggattagc taaaacaatt aatgggaaaa agtattataa ttatttgctc tttgataaaa
    14881 acatttatcg aggctttttc atgcagtcaa tcaacgttat gaatgttaaa ctagatttta
    14941 agtatgcagg atcaaaaaca aaagtagctt tgagtgaacc acagccagaa attaattcaa
    15001 aaatgagatc gatgagagca gctggtattg agttgaatga tacatttcta tctatttaca
    15061 gtttaaatgg acagtatcag caacgtgtgt cttggtataa tgacaataat gaatctgtcg
    15121 gtgaacgtaa tattgatatg agagaatttg ttgggtacga aaaaatggat cgcttacctt
    15181 attttgtcac aacagataca gcatgtgcag aatacaaagc tcctgcgtta tcaacaaaca
    15241 atttaacttc aaaagtagtg ggaggacgtg cagaaaaggc ttatagctcg aatgatcatt
    15301 tcaccgatgt tgtaggagct gatacttatc acagaagtgg tgtaacgtat acgcttcaag
    15361 gcgcttcccc aacattcatg attggcgcaa atacgaatag tatgatgttt agctttgata
    15421 ctgcattgct ttggacacca caaccatcga agcctacaaa agaagtgttt aacaaagcta
    15481 atactgaaga ggcagcacac aatattgaca aaaaagtgat tccacaagga tcagatgttt
    15541 actatcatat tcaccaaaag tttgatgcat taacagtcaa cacaatgaac aaatacaaat
    15601 catttaaaat cactgatacc tttgacagca aaaattttga tatggtatcg gatgggaaaa
    15661 actatgatgg cgcattgtgg tatgttgaag cgaatgggac ttggaaaaaa gctgatacag
    15721 gaggaaaatt aactgtatca ggtaatacag tcacttatac tgcttctgac agctttttag
    15781 ctaatgctgc aaattacggt agggactaca ttttgcagct ccatatgaga gcaaaaaatg
    15841 atgcaaaagg aattgcacca aatgtggcta ctgtaacgtt taacaatgat ccgaacaaag
    15901 gaacacaaaa aacaaataaa gtagaaaact atttctctct taaaccaaat aaaaaagtgg
    15961 aacaagatgg gaaagatgtt gatggtcgaa atgatggtaa aaaaggaacg cctacagctc
    16021 ctttaaatgc aggatctgaa gtacaatatc tagtcacaca aaaatggcat acaaaaggtg
    16081 tagacattgc gctcgatcat tacaagcaat ttagtattca agatccagtg gaagctcgtt
    16141 tgacgtataa agaaggttct gcgcgagtga tagatatgag tacaggaaaa gatattactt
    16201 cagaaggtat actaacctat gatagccatt caagaacgct gaaatgggaa gcttccgctg
    16261 atttcttaaa taacaatctt ttagatggac gagaaattca actgatattt acagctaaaa
    16321 ctcctttgaa tgaagaattg gatatcgaca accaagcaaa agtttcagta gaaagtattg
    16381 agaaagcaac taatgtagta acgattgggg taaaacctaa cttacctcag gtcattgtgc
    16441 caaaaactgg atcgaatcat ttagtgctaa ctgttattgg aagtatatta tttcttactt
    16501 tagcatcatt gggctatgta gttttaaaac tcaaataaat ataaaaacaa ggctgatttt
    16561 cagccttgtt ttcttttatt tgaagattgg attcgcacta ataaatacat tatctacgcc
    16621 aattctacca gcagagtcaa ctacaaaatt atgacctaat ccgagaatac tttggttatt
    16681 ataaagtaaa tttgcattat gggcagggct atttttccat cctaccatgg catcgatagc
    16741 accatcgtct gataaaaggt catcaatata cagcccagtt tgacttatgt tttctccctt
    16801 agcaccatag cagcctaagc tttccaataa atcttggaaa gtaccaccac cagggcgata
    16861 gtgactgaag ctgacttgta attctttagc tcgaatatct gcagcctgtt gaacaatatc
    16921 attgaattgc attggggcaa caccattttg ttggcgataa tcatttaacc aagcaagttc
    16981 tttttgacgc ataaagttac gtaattcgtc taatgaaatc tttttatatt tgaatgtgat
    17041 tgttttattt tgatcgacgt tattaatgga tcgttggccg tttttatcca cggtaacggt
    17101 aggatcgtca tttctgcaca agaaataatt ttgatcagtc atatttttcc aaggagtaga
    17161 gaagctatcc ccttcgtata ctgtttttga ttcactagag agaacagcgt tggtatcagc
    17221 atttacatga ttaacagtca cattaaattt acgagtgtag ttgaaaatat aattttgact
    17281 ttcagtcaca ttgtttaagg ttactgaatt tgttggttct acccgatcgt ctaaaacata
    17341 tccttgaata gaaggagcag ttaaagtgac actttcaccg tgagtgacgt tgattatctg
    17401 ttctttcaaa atatcaactc gcccctcatt tttgtagttg ttattgcaca acaaattgat
    17461 ttgatgcact tgattataat tgaatgtgat tgttgtatta cctgtgattg ttttgctttg
    17521 agtatcatta ccaatcactt tgtagctaaa attatcttta gcaccttcgc tatatttaaa
    17581 tgcttttgcc ttagcagtgt aagtagaacc agaagtcact tttgcttgat ctgtagcaat
    17641 cacttgacct gtttgattgt caatatggtt aatggtaacc atatattctt ttggcgtttc
    17701 aggtttgtgt tgcaaccctt taaatgcagc atcaagacta tttttagcat tgtttacttg
    17761 tgtttgagaa accttagaat cgtctagtac ttttttagca ttggataaag cagtctgaaa
    17821 attattccaa gattcttctg taaaatctcc tttagcagtt gattttactc ggttgtataa
    17881 agcttgtaat tcagctttat tcacagtagg aactggtttt ttctttaatc ctttgtaagc
    17941 agaatctaat ttatttttgg cactatccac ttgcttttga gtcgcttttg agtcatttaa
    18001 cactttctta gcattgttta aagcggtttg gaatgcgttc caggtattgt cggtatagtc
    18061 accttttttc gttcccttaa cgctcgtata aagagcttct agagccgatt tattcacttt
    18121 gggtttagag gtgtcagcgc cataccaacc gataccttcg tatttccagc catagtcttt
    18181 ggttaaccat tgtacttctt tccaacttgc agtgtagtga tgtgagccag attttacacc
    18241 aggaaggaaa aggcggtaga tcggtttatt atttttttcg gcagaacgcc aggcaggccc
    18301 ttcataagtc cagccgtaat ttttagtcaa ccatttcact tcatcccaac tagctgtgta
    18361 atggtgatca cgaagacctt tattgtacaa acgatagaca agatggccac cgctagtcgg
    18421 tgcttcccag gcggggcctt cataatttcc ccattttatc tttactagat ggtcacgttc
    18481 ttttttgcta gaggtgtaaa aatgttcatt attatttgga ttgtaaaggc gatacatgac
    18541 gttagttgaa gcagcgtcaa cagatccagc aagactaagt gaggcagcaa atgttccaat
    18601 agctaaaaca gtttttggat taaataattt cattttttca actcctaaat ttaattaaaa
    18661 atgtttacat gattattata tatcggttaa ccgatatagt caataaaaaa taagaaaagc
    18721 cttatataat tttggctttt cttattttat ttgttagttg aagtattaat atcaacgatt
    18781 tcttcagcca atttatttaa tttcatagca tttatgaatc tcatgttgtc tatactagag
    18841 cgtttgttgg caaggtcact tactgtagtt aagggtacgc cagattctat ggatatagaa
    18901 cgaactgatt taccactatc aaacagcaac cattcgattt tctttttatc agcgtacatt
    18961 tcaatgctcc ttcttgtttt ttctttatta tagctctttt ttgtaatgaa tcacaatatc
    19021 aagagaaaaa ttttctatag aaaggtggat ttttatgagt attcgttctc cgtgtatcca
    19081 aatgaggttt gttgctactt tcgttatttg atatactaaa aataaacaac gaaagaagga
    19141 ataaaatgaa gaaaaagcaa ctatggtttg tctctatgtt tgcaatattt tttattattt
    19201 taagtggatg ttcaagtaat aagaaatcgg aagctaaaga agataatagt ttattgcaac
    19261 caggaactgt ttggaaagaa gaagtaggtg gtcttattca taatttaaaa attatagatg
    19321 atactacctg ggaatattct gaaagtgtat ggcatccaga tcctgtacaa ataacagtta
    19381 agaggcaaaa agactatgaa ggtttagaaa gatacaaaat agtagattct gcaggcatta
    19441 gggaatttat aaacaaacct gattcattat ttattgttgt tccctatgag aaggatggcg
    19501 taaaaaaaat aatttttctt ggatctagca aggatgagaa gcgaacgaaa gagaaattaa
    19561 ttcatgatgg atcacaatca aataaataca aattacaaaa gactagcgag tgattgctag
    19621 tctttttatt tgtcctgaag gaggaatcac agtggcaaaa gctaaaacgc catttgaaaa
    19681 agccgcgtct aaaatcttag aacgccgcgg tgtatctgaa acacagtgga aacaagaagt
    19741 ccttgaaaaa gcacaatttg acctattacg gggaaaagac aaggacttag aaggatatat
    19801 ctatcaacgt gaacgtgaaa agcttgtttt cgatgaaatt attaaacact aaaaggagcg
    19861 aacaatttta tgtttgaatc aattacaatg aattttttaa cacaaccgaa agtagtagct
    19921 gcggttgata tttttagagc attacaaact gccgcaacta gtttgaaaaa taatctgatg
    19981 atttttgctg ctgttgcggc agtggcttcc attgctcttg gaggtatttt cttcttcttt
    20041 gggcgaagag gagctgaaaa cgggaagaca cacgtaggtt atgtattgtt gggggtagga
    20101 gttattttag ctgccgcttc tattgtagtt tcaatgttct caatgtttgg acaaacccca
    20161 caaggactat aaaaaaacaa gtagaaggag taaaaagtac gtggctactt tttactcctt
    20221 tttttatgga aggagaatat tcatgttttt tacgatttca ggcaatgcaa aattggataa
    20281 agagttaata agaaaagcaa cgaagaaagt gggaaagcgt acttcctttt taccgaaaac
    20341 aattaaatat tctgtcattg aaacggaaga acagctacat gtgttaggac aagtgttaga
    20401 acaagagaat caatcttctg ttaaattgac tctcacgtta catgataaag aaggaaaaat
    20461 agaaaaggct ggcacagaat acgtttttgg ctcactggat tatcaaattt cagaaggttt
    20521 ttatcctaca ttgattgatt taatccgcct agatatattt aaagatacgg atgaaaccat
    20581 gagtgtggaa gagtcttatc gaaacgaaaa aattttacaa gttttagaag aagagttaga
    20641 aagcgaccaa agaaaagtga atcaagtgag tgaagtcgaa gcaaacgaag aagtaccatg
    20701 ggaacgccca atctttccaa aggatgaatc aaaagaaaaa gagaaaggaa ttattgacga
    20761 acaagctgac attttgactc ataaatcaat atttgaggaa gagaacgagg taatgggtca
    20821 attggaggta gatccctttg aagaaacaat ggaggatgaa gagacggccg aagtagaagt
    20881 tgatccagtt gaagctaatg aagacccaac atggactgaa ggggattctc aggaatatat
    20941 tgaaccactt atcagtgaag aagtagagaa aaatgaaaca gagatacaaa aggcaacggt
    21001 atttgattat attttggatc aatatccgcc agagcatgaa tggatgaaag aaaaaaataa
    21061 attattttta aatgaacttt attcgaaata tcaattacct caaacctatc aagcattttt
    21121 agagagtaaa gaagaattaa tctcacaggg taaaggcaca cttttagaac agttagagtc
    21181 tgtccaatca acaaattggc atgagcttgc acaagaagat ttattagcta cttttgaaga
    21241 acgtcaacaa gaaacagatc aagaaattaa tgagtatgcg acggaacagt cgtcgaattg
    21301 ggagaaagca aaagaggaac taaataaaga agaacagcga gtgattgaag aagaagtaca
    21361 aaaaattaaa gagcggtacg aaaagaaacg tgacaatgcg tattccttgt gtgtagaaaa
    21421 gatcaacaat tttactcaga cgaatcaaga agcattatcg aaagaaaaag aaaacttatt
    21481 aaaagaacgt ataaaagaac gaaaagaaga attttaccga gggttgcgta ctgataagct
    21541 aaggattagc aaagaattaa atgaacaact gaataaactc tatcaaacta cgactgaagt
    21601 tctgatggaa aaacacgagc agatccaaca agaattacaa gcacaaattc caaaatggaa
    21661 aaaagagtat gaaattcacc aacaagaatt gaaagaacaa gaagaaaaac aacggaaaaa
    21721 agaaaaagag cgcgaacaat tagaactaaa aaaacgtgaa caggatcttg cggaacaaaa
    21781 aatggcgata gaaaaacaac ggctagaaca agaaaaggaa aaagaagcag ctgctcgtaa
    21841 ggaacgtcaa gaacaaatgg atacattgcg acaagcacag ttaaattatc tgacacatcc
    21901 tcaaccagta gctgctgttc aaacgccagt gaacaaccaa gcaaatttaa ctccttcacc
    21961 ccagcgttcc tttaacggat ggatggtggg atgtattgca agtttaagct tgcttctagg
    22021 tggaggaaca gtatttgctt ttaatcatat caatcaatct tcacaggctg attctgtagc
    22081 cacaacggcg tcaatcaagg aagaagccca ggaacaagca agaaaagagt atgaacaaca
    22141 attagaagaa ttattgaatc gtaccaatca gtcaacaaca gagtcaagca tggaaaatag
    22201 caaagagagt gattctagta aggctaaaga aggatcaagc gattcaactg tacaatcaaa
    22261 cacttccgaa agttcagaat caaatgaggt gaaataatgc gtgttagaga aacaacgaag
    22321 cgaaaagaca aacgattcaa tttagaaaag aataggtatc tttctcattt ctatcgctat
    22381 actccctcag gagaagagct accagaaaaa catgacaaac gcatttcgaa aaagattttt
    22441 tatagtggac ttgtatacct ccctttagag tatttagtaa caagagtacc agaagttttc
    22501 aaccgattgg aaattgttaa gggaagctta caaggaataa atctgtttca attacaggaa
    22561 atgccacagg tctttcattt tgattcagct ggtttttgga atccatttac tcctacaact
    22621 agtttgggta cacgtttagc aatttcgaca gtttgtgtgg ttccagctgt acttatggga
    22681 ttgaaaatgg acatgtggtt tcgtcctcta gcagatggac aaaaagggga taatcgatta
    22741 atgaccttaa aagaaatcca acagatttat ccgcaaatac ctgaaacaaa aactactttt
    22801 ccaggttatg gaggtattcc aatcacacat tacaaaaaat attggtacat tcaaacagat
    22861 actgttaata cgtgtattgt tggtacctct cgatcaggga aaggtcagat tgaagtgtta
    22921 gccaccattg acaatttatc tcgtgcagaa aaacaaagtt caatggtcgt taatgatcca
    22981 aaaacagaat tatatgttgg tagtaaagat gaactagaag ctagagggta tgatgtatat
    23041 gcctttaata tcaccgatcc actgcaatca atgagtgata atccattggc actcatcgtg
    23101 aaatactgga aacgaggaga catagatact gcaacacagt taaccaatac tttcacaaat
    23161 acgatttatt atgatgccaa cgcttcagat aacaaatact ttaacgaaaa cgcgcagaaa
    23221 gctgtcaatg cgcttatttt tgcgttgtta gaacaagctg atcaaacagg agattactcc
    23281 aaattgacac cgaataatct cgttgagctt ctttctgaga ttggcggttt taattattat
    23341 ccagatccag acaatgaatt caaacagttg aatgccttag atgaatttat gaatcagttg
    23401 ccacctggaa acattgcaaa aaaacaatat ggggcaacga aaattggatc tgataaagcg
    23461 aagggaaata ttttatcaac ggcaatcacg ggattgaatc catttactct aacgaaaatt
    23521 gcaaagatga cttctcaatc aacgattgat tataaatcgg ttgggtttcc aaaatactta
    23581 gatttgaaat tacatgaaag tcttttaaat caacgagtcg tagtagaatt ttatcataag
    23641 ggaaagaaat ttcatgaaga acgtgtcaaa gtaggctata aaggatttag tgaaatcaat
    23701 tttgattgtc atttagttgc aggtgatcaa gtttgtttaa aatacgatca tcaaggaaaa
    23761 gaatatgtgg cttggtatga attttctcaa agagtattga aagacgaaga gggtaatatc
    23821 gtttatcgga aaaaacgagg agaaacccat ttgccagaat tagaaaaaga agccgttcta
    23881 acattagatg aggaaaaaag taacttatta gtagagtcta ttcggatgca ctataccgat
    23941 aaaccaatgg ctatttttat gcttacacct gattatgatc catctaacca tatcattgtt
    24001 agtatttttc ttagtcagtt atataaagaa ttatctacgc aatgtgttaa gactaaagga
    24061 gataaatgtc accgcaggat tcattttatt ttagatgaat ttgggaatag tgtgacctgt
    24121 ttagcctaag gataaaagag gctagttcca acgaacattg gaataacttt actatcgaga
    24181 aatcaaatga cggggtaacg ccctgaaggg tttccactga tactgcgaca tgcgttagta
    24241 ggatgtgcat ccaaacgcgt atgaagctcg ctgaagtcgg cagaagggta ccgtaaggaa
    24301 agacacgata cgtatctacc acgcaagtgc tctagaggta gggtatgtac ctgatatgtc
    24361 gggtgtccta aaaagttcaa taaggtgaga atgatttgat tatctgacaa acttgcgaat
    24421 ggaatgtctg aaacgaataa ctcatagaaa tgtgaggggg aagggggata cgtcctcttt
    24481 ccattgactc gaggttgagt aaaaatgtgt ttctatgaaa aaccttgcat ggttaaaggc
    24541 tatggagtag tcaacaggca aaagcaagca cctaagttga aatctatgga tagatagtaa
    24601 ggaacagggg aagcatctag cacggaggga gcataccgaa gatgtgtttc taaggaaaaa
    24661 ccgaaatcgg ttataactta ggtgaataga tgtgaaagta gagccacgac cagtgaaaat
    24721 ttcgtaatga aattggagga acaggctcaa gtcgttattt ataatataag aaacctattt
    24781 aaaatcatgg attctcgtaa gactaaaaag gtcacgaacc agaatggagc gtgataaaac
    24841 catgactaac acaaaaggaa ttagcttaaa gaacaaacaa ctcagacacg ccgaatacta
    24901 cgacatgact gagactttcg atgaattgca cgcaatgagt gttgcaaaat caaattttaa
    24961 aaatctcatg aaattcatta ttgatgacaa taatatcttt ctagcgtata gaaatatcaa
    25021 aaagaataaa ggaagtgcaa cggccggata cgatggtgtc actataaaag acattgaaaa
    25081 attggcatgt ggtgactttt taactattat taaaaggcga tttaaggatt acagaccaag
    25141 aaaagttaaa agagtagaga tcccgaaacc aaacggaaaa cttcgacctt taggaattcc
    25201 ttcaatatgg gacaggatag tccagcaatg tattctacaa attttagaac caatatgcga
    25261 agcaaaattt aataagcata gctatggatt cagaccaaat tgttctacag aaaacgccat
    25321 agcagattgt cttgctagaa tcaatcaaag taagatgaat tatgttgtag atattgatat
    25381 aaaaggattc tttgacgaag taaaccatac caaacttatg cgtcaaatat ggacaatggg
    25441 gattcgagat aaacaactcc ttgtaattat tcgtaagatg ttaaaggctc cgattgttat
    25501 gcgaaatggc aatgttgttt ttccaaccaa gggtactcca caaggaggga ttttaagtcc
    25561 actgcttgcg aatatatgcc tgaatgaatt tgattggtgg atagctaatc aatgggagga
    25621 acgaactgtc aaagagctga aacaccacta tcgaaaaaac ggagcatggg ataaaggaaa
    25681 tatctattcg aaattaagaa gccacacaag tttaaaagag atgtatctgg tacgctatgc
    25741 agatgatttt aaaattttct gccgaacagg agaagaagcc caaagggctt tttatgccac
    25801 acaaaaatgg ctcagagaaa gattaaagct cccaatttca atggagaaat ctaaagttac
    25861 gaacctaaat aaagctgaaa gtgaattctt gggtttttca ataagactcc acaaaaaggg
    25921 aaaaaagaaa gtggctttca cgtacgtttg tcgcaaagca gaagaaaaca ttaaaaataa
    25981 actaaaacaa cagattaaaa aaattcaata cgctggagca aaaaaaaata ggcaccgagc
    26041 aataggtgac tacaacatga tggtcatcgg tatccacaat tactatcgga tagcttcaca
    26101 ttgtagtgtc aatttcactg agatttatag aagtgttagg acagtaatag aagcaagact
    26161 gaaagctaga ggaatgacga aaaatggaat ctatggacaa gatcatgaag gataccgtcc
    26221 gtatctaaag tctaagcaaa tgagatatat tgaaggtttt ccgatacttc caattggcta
    26281 tattaaaaaa agaactccac tcaacaagcg gagaaatcta aataaatata ccaaggaagg
    26341 gagaaagctc attcatcaag agcaacaagc tatagaaagc tggaaaatca agtatcttgt
    26401 aaaaggtttt agtaacaata agaaatacac ggtggaattt tctgataaca aaatatctaa
    26461 gtttatttcc caaaaaggga aatgcgctat cacaggaaac gaacttttga tggaagaaat
    26521 tgaatgccat cacattaacc catatcattt gtccaaagac gatagttaca agaaccttgt
    26581 cattatctct agagaaatcc atagattaat ccatgcaact agtgaagaaa ctattctgaa
    26641 actgcttcgg ttactgaaac taaatcacta tcaaattaga aaactaaaca cgtatcgaaa
    26701 aaaagcagct aattttccaa tatcaatcaa aatgaattag ataggaaaca aattatattg
    26761 taattaacga tggaacgccg tgtgcggtga aagtcgcacg cacggtgtgg accgggggaa
    26821 aagacggaga taatatcaaa ctcttaccta tcggaattgc caccaatgga taatatggaa
    26881 ggaatcatga cggtcacagc tggccgaaac atgttgtggg atctgtttat tcaatcttat
    26941 cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga aaattgtcag
    27001 acacatattt atattatgtc gacagatgat aataccatcg aagaattttc taaaaaagtg
    27061 gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat gaatactcat
    27121 atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc tactttttta
    27181 gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg tcataaaata
    27241 cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta tcaatatatt
    27301 gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac acatgcggac
    27361 ttggatctta aggctttaca aattgattat gattatttcc taccagatga atatgctaga
    27421 aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat
    27481 aaaatcgttc aatctcttga gaatgagacg gctaaaaaat tgttaattca attttacgaa
    27541 gaacaagcag cagatcgact tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt
    27601 tctgaaatga ttgggaaacg gttgttattg gaatctgaaa aggtggtgaa agaatgatca
    27661 ttggaggaat tttttctggc atagccgact ggtggtcagg cttattcgga gaaggggatg
    27721 caaaccccaa aaccgtaacg aaagtcttac aagattatgg taattatctg cagtatcaag
    27781 atgtgttcag ttggatttgt aatacttttt tatgggggtt gatcaaaggg ttatataatc
    27841 tcaatacttt actagaaaaa accatttatc aaagctttga tttaaaagac ttattgaacg
    27901 ccgctggggt aaatgaacta tttcaaagtt tgattagtaa ggtattggct cttttttgtg
    27961 tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca ccaaaagtca
    28021 aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggt agttctttaa
    28081 tcgatgaagg gttaaatatc agtaaaagct tttacggaga cgtcactgca gcaaataaag
    28141 cagataaaac gggttcacct gcttttcaaa ttattcaaaa taatgtgatc gatgtcagta
    28201 cattactagc aactgacccg aataaagtag aatccctgcc aacagacaaa agaaatgctt
    28261 taacagcgaa aaattttaaa acagcgaata tcaatgggat tattactcct aaacaagcta
    28321 aagaaatggc ggataaagat aagagtggtt tatcagattt acaaaaggat cggttaagag
    28381 cgttacaata ccgactggaa ctggatgata atggaaaaga aattcctgtg caaatcagtg
    28441 atgaaggctt agccaaatat gtttatactt cagggtatcg tcgatacatt agtagtccag
    28501 ggattatttt agctggtgaa tcttctttgg cggtggccta tttatttatc ctatttacga
    28561 tcgttacttg cattattgag ctggtattta aaaaattcta tttagtgatt gccgcgtcaa
    28621 cggactttga gacaggacaa cggatgaaaa gtgctatcga agatatttct caatccttct
    28681 tgttattggc atttacaatt ttagaattac gaatctatac cttaatgctt tcaggtattg
    28741 gtgatttgca tgcggcagga aagatcaatg gattcttgta tgttgtcgct ttgattgtac
    28801 taaccattgc gctattcaaa ggctctcaat cagttacgaa aattttcggc gttgatacga
    28861 gtctgaaaaa cggcagtaat tctcttctta gcatgtttgc cttaggaaat attgcgaaaa
    28921 atgtgggtag cggagctaaa aaccttgcaa gtgctggtaa gagtggttta agccatttaa
    28981 atgatattcg taaaaatgga tttggccatc gaaaagatgt tacccctgat aacggggaag
    29041 aacaatcaga cgataaagct acaactgtac ctaatagtaa gaaaacagtt tccgaaatgt
    29101 tcgggaaagc gaaaaatggt gtttctaaag ccgcagaagg tcttggttac gtcaatgaac
    29161 gtgggttaaa aggaacagct gaagatctta cagaaaaagc caaagacacc ttagaagata
    29221 aattagacgg cacgagagct aaagaattgg ctgaagctgt gaagaatcca tccgaagcac
    29281 tagaaaaaca aaaagaaaaa gcttcagaag tcaaagaagg actaaaagat gcgtatggtg
    29341 aaggacaagt caatgcagca gtgaaagcta atacaacaac cgaaaaagaa aatcaagaag
    29401 gtatagtgat tccttcagtc aaagatcacc aagaagcgtt gcactctaat gaaaattcga
    29461 ttgaagagaa aaattccata gaagaaagaa attcagaaga aggaaaaaat ggacagttac
    29521 caaaaactga tccagaacaa tatcgctata aacctaacag tgaccttgaa aatgagaagc
    29581 gaatttcaga tttatcaaaa gtaaatcaag aagacaaagc agaaaatgag ggacagttac
    29641 caaaaactga tccagaacaa tatcggtata gatctgttaa ctccgaaaat gaaaaacgca
    29701 tttcagattt accaaaacga aatcaagaag tacaaacgga tgaggattcc atcgttctgc
    29761 caaatgataa tggaatcgaa gttggaactg aaaaatcaac gaaaattggt ttacctaagg
    29821 cacaaaattt accaatgaaa gatagtcgtt tgaatcaatt caaggataat gatcaaagtt
    29881 ttgttcatca atcacctgca ggtactgttc aaatgaaacc aagtcgtttg cctgaaaatg
    29941 gaaatctgac aactgtttct tacaatttat ctgaaagtgg tgagacaggg aatgcgtccg
    30001 ttcaaaatgt tgtgacaaga gaagctgcac caaaacctat tcaaaacgtt catacaggtt
    30061 acaagccagc aaatgtggca tttactgcac caacaaaaat aaatgtgaca agagaaacta
    30121 aaaacaggaa caatgcattg aaagaacaac tattgaggga tatcaaacag gatatcaaac
    30181 cataaaaggc ctacaagtta ggccttttct ttatatgaaa aggagaaatc aattatggat
    30241 aaatatataa acatgaaaaa ccctccaaga tttagtggag aatggaagtt tttcgctttt
    30301 ttctttttag atctattaac gcttggtggc tgcttttttt gggcgaaaat ggtcaataat
    30361 cttgtccact taccactctt ttttgcactc ttgaattatg gcttatggat tgcgatagcg
    30421 ttagtgattg tttggcgacc ctataataac ccaggtcaac gacaaatttt tgcgattatc
    30481 gagtctattt tttttgcgga caagaaccat tatcatgcgt tagatcctaa ccgaaattat
    30541 catggtacgt ataaggagga tgaataaatg cgagtcaaag gacaaaattc agttgaaagg
    30601 aaatccattt tgcagtttgg aaaaccagaa gagcgctcta tcttggactt atttccgtat
    30661 aaatcctgta cagaagaggg agtcttaatt actagagaag atcattttca acgttattat
    30721 cgtgtagttt ctacagacgt ggaaggatta aatgaacaag agaaactaga acgaatgaat
    30781 cagttgacca ccgtcatgcg tacgtatgtg ccaacaatta agttagttag tttgaccaca
    30841 gagacggatc taacggaaca aatcgtgcaa aagcggcagt tattagataa aaatcgtatg
    30901 gaacagtcct taggaagaaa tctacagcga ttaagaaaat atgaaaagaa attagtagag
    30961 gaaatccagg aattgaaacg agcagaacag gaacgccctg atttaagctt ctttttcgtc
    31021 attgaaggaa agacaatcaa ggatttgcaa accagaaacc gtcagttaat gcgatctagt
    31081 ggcattttag ggttaaaacc actctacaaa aaagaattga tcaagattct ttatcgaatg
    31141 aataatatga atgatgagta ggaatgggga agagagaatg aaatatccgt tgatatggtt
    31201 agaaaaagat gttccgaaag aagagaccac tagaaaattt cctcacactt taagcctggg
    31261 taagttggga aaaggaaaat cttttaacca agtaaagaat caaacaagaa gaggtgactg
    31321 tacatgttag aagcaattaa aacagctttt tcaccacaaa atgatcaaaa ggtacttgat
    31381 ccattaaagc aactaggaaa tcgggaaata gaaattttaa aatccaaagg gtatgattta
    31441 gactttttaa aacaagtgat gccacaaggt ggattaaatt tcgatgaaga gtatgtgacc
    31501 tatggaaatg gttattcacg atgtgtgaca gtttatcact acacaaaaaa tcctacgctt
    31561 ttttggttgg ccaatctcat gaacaatcct catacagtgg caacactaga tgttagcaca
    31621 gatgaaaagc aaaaagtgat tgatcaaatc aatcgtgcct tagatgaatt aaatgatcgg
    31681 tcagataatg aacgaaaagc gactgaccga aataaaagcg cggaagaata cctagaacta
    31741 gcggaatatg cccgaaaatt gacttctaat ggggaaattt ctaaacaagt caaagtgaga
    31801 gtacatgttt acggaaacac gattcaagaa gtagatgaac gagcacagga actgatcgaa
    31861 aatctcaaag ggatggatta tcgtgcagtg acctatctgc acaaaacgaa aagagactgg
    31921 caatcgatgt tcaccatgtt cgatgaacaa gctgaatggt ttggaaatat caaagggcaa
    31981 tcactaccat cgctaaacat tggcggaggt ttcccgttcc accaccaaac gctaaaagat
    32041 ccaggtggaa cacaatatgg ggaaaccatg acaggcggcc cgtttgtttg gaatgccacg
    32101 tataaaaata gtgtgcggtt gtctaccaat accttgcttc taggaatgat gggagctggt
    32161 aaatcgaccg ttcttaaaat gatcgcggaa gcccatttag ctgcaggaga ctttatttgg
    32221 ggctttgaaa agggaaaaga ctttattccc tttttaaagg aatataacgg cattatggtg
    32281 cgtttagacg gatctgacgg gatgatcaat cctttagaaa tatttgccac acgtacgtat
    32341 gatgaagctt ccagtttata tgacgatgga tcagtcaaag atttgaagat caatgaagca
    32401 gcgtcctatc aaacgcactt agacaaagtg gtctatcagg tacagttagt gagtcctcag
    32461 ctaaaaggaa cgatgaaagc agaatttaag acctatttaa atcgttttta tgaagagtat
    32521 ggaacagtac cacgcggctt tacttcctca aattcgcgtt caaatactga tacgcaagtc
    32581 acaggtaaag atccagaagc ttatccgacg ttcaaagaat ttttagactt tttaggacaa
    32641 ctggaattac caggtgcttc ccaagagaaa aagaatcgaa aagaagaaat ggaatccatc
    32701 gtagaatcct tatgtgagac gtatggcatg atttttgatg gtcatagtac gattcgacac
    32761 cttgaccaac aacagttggt cttttttgac attgactcta ttacaggctt agatcgaggg
    32821 gtacaacaat gtcaaatgta catggctatg acgttgattt ggaatcaggc attgattcaa
    32881 ggaagaagac aacgacgttt aattaaagaa aaacaaatgg aagaacaaga cttcaaatgc
    32941 ttcttggcga tgattgatga gtgccataat ggtgcgacct gttctgcagt gaaaagctgt
    33001 gggttgcaag tagtctaaaa actattgtaa taactcttat aacgagaagt caaatgagtg
    33061 ggtaacgcca caaagggctt ccactgatac tccgacatgc aacaagtagt ttatgcagac
    33121 caacgttgtg tgaagctcgg tgaagtcggc tgaaggatac cgtaaggtaa aagaaatagt
    33181 gaataccacg aaagttctct agaggtaaag ggtgtatccg ataagtcggg tgtccaaaaa
    33241 aacttaatat ggtgagtatg tacaaacgta ctgacgaact agcgaatgta ctgtccgaaa
    33301 ggcatagctc ctagaaatgg gagagacagt gagcaaaaaa tcactcattg tgctaacttg
    33361 tggttgagta aaagtcgttg ttatgaaaga ccatgctgcg ttacaggcgt aggagaagtt
    33421 agggggcaaa agctagcatc taagttaagg catgtggata gatataaggg aacaggggaa
    33481 gccatcaaca tggagagtgc atactcgatg aagtggtagt aagaaatatg tgaatgacaa
    33541 ataacttact ttagtgatgg tgaaagtaga gtcacgactg atgaaggttc tgtaatggaa
    33601 ctggaggaac aggctcgatt cgtctgtaaa agtcatgaaa cccgttcaac catgggattc
    33661 tcgtatgact aaaaaggtca cgagccaatt ggagcgtgat aaaccatgac aaaagaaaca
    33721 agtccaaaag ataggaagtt gcggcatgca gaatattatg gcatgacgga aatcttcgat
    33781 aagatatact ccaaaagttt gaagggagat aatttcaaga atctaatgca gattatcatt
    33841 tctgataaca acattgtcct agcatatcgt aatatcaaga gaaatcacgg tagtgtgaca
    33901 agaggagaag atggagtaac gattaaagat attgaaaatc tcaaccgtaa tgaattcgta
    33961 gaaattgtta ggaagcgatt cgcacattat aaccctcgga aagtaaggcg tgtagaaatt
    34021 cctaaatata atggtaaatt aagaccgttg ggtattccat caatatggga tagaatcgcg
    34081 cagcaatgca tcttacaagt attagaacca atatgtgaag caaaattcaa taatcacagc
    34141 tatggtttcc gcccaaatag atcagcagaa cacgcaatag cagattgtat ggcccgaatg
    34201 aatcaaagtc acatggaata tgtggtagat gtagatatca aaggtttctt cgacgaagtc
    34261 aatcatacta aactcatgag acaaatctgg tctatgggta tacgagacaa acagcttctc
    34321 ttaatcatta ggaaaatgtt aagagcaccc gtagtacttc caaatgggaa gttgatatac
    34381 ccaacaaaag gaacaccaca aggcggaata cttagcccat tacttgcgaa catcaatctc
    34441 aacgaatttg actggtggat agcaaatcag tgggaggaaa aagatgccaa agagctaaaa
    34501 catcatttta ggaaaaatgg agcttgggat aaaggtaata tctatcacaa gctcagaaag
    34561 agtacaactc taaaagaaat atacctagtg agatacgcag atgattttaa aattttctgt
    34621 agaaattata atgaagcgaa aaaagttttc tatgcaacta aaatgtggtt acaggagcgt
    34681 ctaaagttac caatatctga agaaaagtca aaaatcacaa atctttcaaa agaagaaagt
    34741 gaatttttag gctttacgat gaaattagtg accaaaggta acaaaaaggt tgttaatacg
    34801 catataggta caaaagcaca aagcatgatt aaagtgaaac taaaaaaaca aatcaaacga
    34861 attcaaaaaa gtcctaatag caaagatacc tttatctata ttggagtcta taacagtatg
    34921 gttattggga ttcataatta cttcaaaatt gcgactcatt gtagtgtagc aatgcgaaaa
    34981 attgcaagag aactcagacc aagttttgaa atacgactac gagaacaagg aatctcaaaa
    35041 gaaggacaat acggaaagta cgaaggatac aagccttacc tacggtcagg acaaatacga
    35101 tatataagag ggtttccaat attaccagta gggtatattc aacacagaca tccgatgtgt
    35161 aaaaaaagaa atattaataa atacaccgaa gaaggtaggg aagccattca taagaagcaa
    35221 caaaccgtag aagaatggaa gatatcgtgg ttgtgtagca atccagtgct tagtaagaga
    35281 gcaacgattg aatttgcgga caatcgaatt tcaaaattca tcgctcaaaa gggaagatgt
    35341 gcagtaacag gtgaggagct tatcttatct gaaatgcatt gtcatcatat tatcccttat
    35401 catgaaagca aaagtgatag ctatgaaaac ctagtgattg ttactgagga ggttcatcgg
    35461 gtaatacacg ctacacaaag tgaaacgatt gaggaacttc taaaatatct aaaattaaat
    35521 ccaaaacaaa aagaaaaact caatgagttg agattaaaag ttggtaacga agaaatatct
    35581 taataaatat ttgtaggacg ggaaaacaga atgtctttta tagacgaggg aacgccgtat
    35641 gcggtgaaag tcgcacgtac ggtgtggact gggggaaaag acggagataa tatcaaactc
    35701 ttacctatca gtatcgatta atccagattt tattgaaacg gtaaattatg tcacaaactt
    35761 tcaacgagaa atgcgtaaag tagaagcaat gactgtttta gctacacaat ccccacaaga
    35821 aatggtgcca gaaaatatgt cttctgataa cttctcgaaa ctgaagaaag tctttgaatt
    35881 ttcttctacg aagatcttta tgcgtatgga tgaatctatt ttacgtcata tagaaggatt
    35941 agttggtaaa tcaatgacaa actcagaatt agaatccatt cctcaacaaa atcaaggaga
    36001 cgctgtgatc aattttggct caaaagaaac cattcgtgtt cattttagtc cgaacaaacg
    36061 tcaattaaaa ttgtttgatg gaggaaaata atggaaaacc aaaatgaaag tttaatcaag
    36121 cagtatgtta agcgtcgtgc aaagcgacgc ttatttttgt ggttgtttgg tacttctgca
    36181 ggtttgatta ctattttgat tactgtgttc gtgacccttt ttctaatact tgcggcgggt
    36241 tcgattgata attctgattc agattcaagt agtggtggcg aagcttttac aggagaatat
    36301 tcggaaggct taccgattta taaagaaatc aaaggaagag gccctttttc tgatgagatt
    36361 gcgcaatatg ctgtaggtgc agcagtgaaa tataaattat taccaagtgt gatcctttcg
    36421 caatacggtt atgaatcagc gtttggtacg tcggcgtccg caagaaatga tctcaactat
    36481 tttgggatta cttggtttga tggttgtctg tttcctaaag gaactgcgcg aggaatcggt
    36541 ggtattgaag gtggctggta catgaagttt cccaactcaa aagcagcctt ctcctattac
    36601 ggctttatgg tagccacaca atccaatttt aatgcatgtg taggaaataa aagccctggc
    36661 gcaagcttat tgatcttagg acgaggtggt tatgccgctg caggaattac tgaagatagt
    36721 ccgtattata caggttgtat gtccattatc acttctaata aattaaccga atatgatgaa
    36781 ttcgccatca aacattgggg tgaaggtggc aataacaacg gcacgatcac tggtgaatgg
    36841 acgaatccat tccctggcag cagtttagat aaaagttcct tcagtggtgg tcaattattt
    36901 ggaacaaatc caggcggtga attccgtccg aatggcttcc atgatggatt agactttggt
    36961 tcagtcgatc atccaggaag tgaaattcac gcggtgcatg gaggaaaagt cgtgtacgtt
    37021 ggcaatcctg gtatttcagg tttaggtgca tgtgtgatcg tgattaatta cgacggtttg
    37081 aatatggtat atcaagaatt tgcgaatagt acaggaaatt ctcgtgtgaa ggttggcgat
    37141 caagtgaaag ttggtcaagt aatcgggata cgagatacag cacacttgca tttaggtttc
    37201 actcgaatgg attggcgtca agcacaagga cacgcattca cagacgatgg tacatggatt
    37261 gatccattac catctttgaa tagcagcaaa aaatagatca ggagaaaaaa tatggaaaat
    37321 aaaactttaa aaatcgtctt aacagctgca attagcgtta gtttaatcgg caatatcgcg
    37381 ttgttttggc agctgaaaga agaacaagag acaaatacct ttaaaattga agaagtggct
    37441 aaaaacgcgt ctgagaagct caaaaaaaat tcctctgagg cacaggcgaa gatcgacaag
    37501 ttaaatcaaa ccatcaatga gaaaaaccaa gaaatcgatc agctgaagca acaagatggc
    37561 acaaaagcaa aagaggaagt aactgaagca caaagaaaga cggcagaaga gttcggtcaa
    37621 ttggccattg ataaatcatt aagtagaaat gaattaacgg aaaaactaaa agatgtcgca
    37681 acccaagaag tgatcgacaa attaagtcct gcagacgatg atcaccaaca tgatgattac
    37741 ggaagttact cctttacatt aggtgacgtg caaacttatg ctcaaacaag cagtgtaaaa
    37801 gaggaacaaa attttgtcgt atttgttgat tatacaatcg gcaatccgca attcaaagat
    37861 gtcaaaccac aaaaaataaa aggtggatta actgtcactg aaaaacaagt agatggtcaa
    37921 tggaaagtca tagactttag ttatttcact cgttaggagg aagaaaatga gtaagaaaaa
    37981 aggagaaaga caacgtttca aaaaacgaaa aagttatctt caatggctgg gaatctttat
    38041 gattctcggc tttttgattc ttgctagtcc gatttatacg aatcagaagg tagatatacc
    38101 agatgaggta agcacgaacg cctttttaaa cacgaaagag ctgacaatgg tttcaaaaga
    38161 aaaaggagcg aaccagcaat tagtttttga atttgcgatc gatgatgaag atcaaagctt
    38221 gttaaaagaa ctggctaatc ttaattatcg cgtagaagta aagacagcta aaggggatta
    38281 tcaagcaata cgagccaaag tgatcaaggt gtctgatgat tattttgcag tgaagctaac
    38341 gcatgtacca gaagaatata tcgccatgcg tttaaccatt attcctgaaa aaattgatcc
    38401 aaaagtggat atgcaagaac cacaagatct gatttattac gtgcacgaag ataaggttaa
    38461 agatcaagtg aaagatgaag attatgaaca acacgcgatc aattacaaag tgaaaggata
    38521 caagaaagaa caaaaagcag ctcaaaaaca aatggaatct ttccaagcaa ccattgattt
    38581 gaatgaagaa ttagtgaaga agctaaaaga tcaattgcct tatcaagtcg cagatgatca
    38641 ggaaaatact gaaaacaaga ttaatagcta ccagcaagaa attgaaacat cgaaaacgca
    38701 actgaaagat caagaagaga tcattcaaaa acttcaagaa aaaattgatc gtatgacaaa
    38761 agaaaagatt tagaaggaca ggtggagtga aaaaatgaag cgaacaaact tggctgacaa
    38821 agatactttc gctgagcaga aagtattgat taaagcctat ctcaaagttg taaaagctaa
    38881 agaaatatgt cgagaagtga atttatctaa cacaacaata tctgagttga agaatggtcg
    38941 aaagaattta gatactttaa aattcagatc atttgaagct ctatatgtag ctgctaaaaa
    39001 atattttaca gctgataaca aataaaaaac aggagtattt tatgtgaaaa aagtatataa
    39061 aaaatgctta tgaatcagct atatcacctt aaccatttgt taatgagatg ggagaaaaca
    39121 ttaaccacga ttctaacgca aacatcaagc acataaaaca tttaacactc ctcctttctg
    39181 gctgaagatt tcaaccaaac aggaagacag cgtatcaatt atagcataca ctttttctta
    39241 aattcatttt tcatatgaca aaatatttcc ttctaatcga caaagttaga aggatttttt
    39301 attgtctaaa tacacaaata accttaattt ttaaagagca taaggaaacg tgatatcact
    39361 ttctcacgaa aggtgttagg taggctgtgc ctaccacgtt tctaagaaag ataaatgata
    39421 tcaaaatttt ggatttgtgt gtagatttgt gggtaggatg aaagcgaatg tgggcagtaa
    39481 aataagcgtt tgtgtgtagc aaaatcaaaa aatgtgtagg gatatgtgta gctacacata
    39541 tccctacaca taataaattt ggaggaaaat atggacttat taattagaaa tattgaagaa
    39601 aagtatatta ataaaattga caaacgttgt gaagaactta cggtcaagac tggcaaaaaa
    39661 tggagtcgaa atcaatacct aaaagttttg atcgaaaatg attttgatca cgctcttctg
    39721 aattataaaa aagaccaatt tgatcggttg ttagaaaaat ttgtggatat tcaaacttat
    39781 aatacgaaaa ctttagaaga atatattgca acaaataatc aattaattgg tttgttaatt
    39841 gaataataaa aatagtattg tgaatttata aaaggaggaa atcaattttg gcaaaagcaa
    39901 caagaaaaca tgtatatctt tacgatatgc atatcgaagt acttgatgaa ataatttcta
    39961 ctcatgaaga tatatctaat tatagtgaag cagtcagaca tttgtgtatg atgtatgatc
    40021 caaaaatcga agaaaaaata aatccaattc aaattaaatt gaatgcaatg ggaaaagaaa
    40081 tttctatttt aaccgaaatg gtttcagagt ttgcggatct tcatttgaaa gatacaggaa
    40141 tactaattgg tacaaattca actgtttatc aagaagcaga agaacgtgtg gaaggaaaaa
    40201 ttaaatccaa tcaaaccaaa aaatattctt ctaagttaaa gaaaccagtg aatgaatctg
    40261 ttcataccag agtagatcca tttgatccaa cgaagattct aaaaagataa aagtagaagg
    40321 agaacgtgat gaataaatcg cctgcagtta ttcttacttg tcaatttacc ttgcccaata
    40381 aaaaatcttt ttcaacatac attgattata tgacaagaga aaaagcactg gaagaaatgg
    40441 aacgacgtac gcccgaagaa gaacaggaac tgcaacgaat caagaatgct ttagaggatt
    40501 tttatattcc tgaaggagaa actttttcgg aaagtaagac ggataaagaa ttcagtgaaa
    40561 cgtcgaatga agctcaatcg ttgatggaac atggaatcaa ttttgacaag ttagaggaac
    40621 aagattttac caaatatctt tcttatatga cccgtcacta tgcgttagaa caaaagaaag
    40681 aactgacgca aagggaacaa aaagaatacc aacggttgaa ccaagcaatg aaaaaatatc
    40741 aaacagagcc acataaaaaa gcttcaggaa atcttcctgg agttttttct atggcttcag
    40801 acgaagtgaa aggaagtgat ctgaaggaga tcaaacaaat cttccaaaga ggacaacaaa
    40861 aaggctcaat catttatcaa gatgttatct ctcacgacaa ttcgtatctt gaaaaaatgg
    40921 gcttgtacga tcccaaaaca gataccttag atgaagaagg tttgaaagca gctggaagaa
    40981 aaatgatgga gacattattc gagaaagaac aattgaatga aacaggttac tggatggcaa
    41041 cgattcaccg taacactaag catatccata ttcattttgc agttgttgaa aaagaaaata
    41101 cgcgtaaacc gtatactgag attgaaaatg gtgtgagtta tatcgtaccg aaggggaaga
    41161 gaagacaagc aacattagac gcaatgaaaa ccaattatgt acatgagtta gaacgtttct
    41221 cttatgaaaa ggatcgaatg atcggtatgg agaaacgaaa tttattgaca cgaaaaagtg
    41281 atttgcgtaa caccttaaca aaagtagtaa aagaaccagc acgttacgat cgacacgcca
    41341 tgaacttatt aaatgaagta tataaaaccc tacctgaaaa acgttcggaa tggaattatg
    41401 gagatgaaaa tcgaaccaag ctttcaccat ttacaagggg aaaacttgat gagttaacca
    41461 aatatgtttt aacaaaagaa cctggatatc aagaatatat acagatttca aaagcattga
    41521 aagaagaagc caaaagcctt tatggggaaa gcaaaagaga aacaaagaat gcagaaaaaa
    41581 acgccctctt tgatttaaaa aagcgcacag gaaatgcgat tttaacagaa ctgaagagac
    41641 aggatagtca aataaaacca ttaatagaaa gtgtcaatca gatgcaatct atttacaatg
    41701 aacgatcctt tcgaacactc aaacagttgc caacaaatga atacaagtat agttatcaag
    41761 aattcgtaaa gcgtaggaaa caattccaac aaagagttgt cacgcagcga agcttgcgtc
    41821 aattaaatcg aacaattaat gcacatgaag aaaaatacca cgcaagtaaa gcatatgaag
    41881 agcaacagcg aagaatccaa atggaacaag aaagacaata ggaggaagag aaatgaaaca
    41941 atatttagat caatggaaag tcatagaagg atcactaaaa gaagaacgaa tcgaacaact
    42001 tcctgattgt ttagaaaaag agcatttgtt tcagataagc gaaatgttga gaaacgaaca
    42061 gtttgatcca aatcaatttt tagttgttga gtacccagct actggtgtgt attgttgtaa
    42121 tcatgtgaag ggtgaaaaat attttattat tcaagaatac gaaggtaagt tagcgcctta
    42181 ttatactact tgggaaataa atgaagaggg aatcaataat ttcccatgtg aaagcattga
    42241 agagtctatt tctctcacgg aatgttaaat aaatagagac gtaaaagagt cctgaacgtg
    42301 attgtccagg actctttttc aatgaattgt tttccgccaa ttcactgagt ggtgcgattc
    42361 aattttatta tatcaaaaga aagtgagatt ccaaagtgaa gggaggaaga agaaatgact
    42421 cagacaacga ttggtgttgt ggttgaaaaa agcagggata atctcgtttt tgtgacagaa
    42481 attcaaacag gtagagcttt tgtagtcaca gacaaagctg caaaagcgta tcaaaatggc
    42541 aatattttaa cgttaaatct gactacgaag atgttcgttg atgcagtaga aaattatcca
    42601 tttgtttaga caaacgatat tttacaagga ggaaacggac atggcagaag aaaaagaacc
    42661 actagataat agtcgtttgg gaaagtccaa aagaaaatta gtacggttgc aaaacgaatt
    42721 aaataatcaa tttcaaaagc tatttgatca ccaaaaattg acaaatgggc aaccaatgaa
    42781 tgacaaacga aatggtcgtt catggtttaa ccagcaagaa aaaatagaaa ataaaataca
    42841 aagtttgaga gaagagatca agcaacaaga aaaacaggta gaaaaacttg aaagacaaga
    42901 agagctaaag gaaatgggtt ataacaagta cggtggcctg gacatgac
//
LOCUS       DACDXE010000017        41944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.017, whole genome shotgun sequence.
ACCESSION   DACDXE010000017 DACDXE010000000
VERSION     DACDXE010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.017"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            114..1550
                     /locus_tag="IWM23_000937"
     CDS             114..1550
                     /locus_tag="IWM23_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9883598.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1739..4186
                     /locus_tag="IWM23_000938"
     CDS             1739..4186
                     /locus_tag="IWM23_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9883599.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4416..5483
                     /gene="rlmN"
                     /locus_tag="IWM23_000939"
     CDS             4416..5483
                     /gene="rlmN"
                     /locus_tag="IWM23_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9883600.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5676..6779)
                     /locus_tag="IWM23_000940"
     CDS             complement(5676..6779)
                     /locus_tag="IWM23_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9883601.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7332..7880
                     /gene="aac(6')"
                     /locus_tag="IWM23_000941"
     CDS             7332..7880
                     /gene="aac(6')"
                     /locus_tag="IWM23_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9883602.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            8426..10087
                     /locus_tag="IWM23_000942"
     CDS             8426..10087
                     /locus_tag="IWM23_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9883603.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(10157..10504)
                     /locus_tag="IWM23_000943"
     CDS             complement(10157..10504)
                     /locus_tag="IWM23_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAP9883604.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            10588..11529
                     /locus_tag="IWM23_000944"
     CDS             10588..11529
                     /locus_tag="IWM23_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883605.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            11530..12573
                     /locus_tag="IWM23_000945"
     CDS             11530..12573
                     /locus_tag="IWM23_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883606.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            12579..13649
                     /locus_tag="IWM23_000946"
     CDS             12579..13649
                     /locus_tag="IWM23_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883607.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            13639..14592
                     /locus_tag="IWM23_000947"
     CDS             13639..14592
                     /locus_tag="IWM23_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883608.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            14604..15614
                     /locus_tag="IWM23_000948"
     CDS             14604..15614
                     /locus_tag="IWM23_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9883609.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(15899..16720)
                     /locus_tag="IWM23_000949"
     CDS             complement(15899..16720)
                     /locus_tag="IWM23_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAP9883610.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(16817..17095)
                     /locus_tag="IWM23_000950"
     CDS             complement(16817..17095)
                     /locus_tag="IWM23_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883611.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            17363..19372
                     /gene="metG"
                     /locus_tag="IWM23_000951"
     CDS             17363..19372
                     /gene="metG"
                     /locus_tag="IWM23_000951"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAP9883612.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(19851..20438)
                     /locus_tag="IWM23_000952"
     CDS             complement(19851..20438)
                     /locus_tag="IWM23_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883613.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            20613..21935
                     /locus_tag="IWM23_000953"
     CDS             20613..21935
                     /locus_tag="IWM23_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883614.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            22090..22866
                     /locus_tag="IWM23_000954"
     CDS             22090..22866
                     /locus_tag="IWM23_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9883615.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            22859..23428
                     /gene="rnmV"
                     /locus_tag="IWM23_000955"
     CDS             22859..23428
                     /gene="rnmV"
                     /locus_tag="IWM23_000955"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAP9883616.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            23421..24305
                     /gene="rsmA"
                     /locus_tag="IWM23_000956"
     CDS             23421..24305
                     /gene="rsmA"
                     /locus_tag="IWM23_000956"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAP9883617.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            24756..25385
                     /locus_tag="IWM23_000957"
     CDS             24756..25385
                     /locus_tag="IWM23_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883618.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(25509..26618)
                     /gene="ugpC"
                     /locus_tag="IWM23_000958"
     CDS             complement(25509..26618)
                     /gene="ugpC"
                     /locus_tag="IWM23_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAP9883619.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            26827..27249
                     /gene="mgsA"
                     /locus_tag="IWM23_000959"
     CDS             26827..27249
                     /gene="mgsA"
                     /locus_tag="IWM23_000959"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAP9883620.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            27572..28108
                     /locus_tag="IWM23_000960"
     CDS             27572..28108
                     /locus_tag="IWM23_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9883621.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(28148..29911)
                     /locus_tag="IWM23_000961"
     CDS             complement(28148..29911)
                     /locus_tag="IWM23_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883622.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(29914..31629)
                     /locus_tag="IWM23_000962"
     CDS             complement(29914..31629)
                     /locus_tag="IWM23_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883623.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(32408..32632)
                     /locus_tag="IWM23_000963"
     CDS             complement(32408..32632)
                     /locus_tag="IWM23_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883624.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            32832..35600
                     /locus_tag="IWM23_000964"
     CDS             32832..35600
                     /locus_tag="IWM23_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9883625.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            35681..36100
                     /locus_tag="IWM23_000965"
     CDS             35681..36100
                     /locus_tag="IWM23_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAP9883626.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            36112..36582
                     /locus_tag="IWM23_000966"
     CDS             36112..36582
                     /locus_tag="IWM23_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883627.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            36599..37378
                     /locus_tag="IWM23_000967"
     CDS             36599..37378
                     /locus_tag="IWM23_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883628.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            37365..38186
                     /locus_tag="IWM23_000968"
     CDS             37365..38186
                     /locus_tag="IWM23_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9883629.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            38203..39252
                     /locus_tag="IWM23_000969"
     CDS             38203..39252
                     /locus_tag="IWM23_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9883630.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            39265..40269
                     /locus_tag="IWM23_000970"
     CDS             39265..40269
                     /locus_tag="IWM23_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9883631.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            40902..41921
                     /locus_tag="IWM23_000971"
     CDS             40902..41921
                     /locus_tag="IWM23_000971"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAP9883632.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        13759 a         7374 c         8672 g        12139 t
ORIGIN      
        1 aaactaagcg actttttcac aacctccttt acataatttt attttgttgt ggaaggggtt
       61 tcgtgttatt ctaaaagagg aacgattgaa ggttatagga aaggaagaga tgaatggaac
      121 acaaagaact tacctcattt ccaaaagatt ttctgtgggg ctctgcttct gcagcgtatc
      181 aagtagaagg agcttggcaa gaagatggga aaggagaatc agtctgggat cgatttgtac
      241 ggatacctgg aaagacattt aaaggaacga acggtgattt ggcagtagac cactatcatc
      301 gttataaaga agatattgct ttaatgaaag aacaaggtct gaaagcttac cgcttctctg
      361 tagcttggac acggattttt cctaatggac gaggagaagt caatcaagct gggctcgctt
      421 tttatgagag attgatcgac gaattgattg aaaacgatat tgaacctatg ttgactttat
      481 atcattggga tcttccacaa gcattgcaag atgaatataa tggttgggaa tcacgtcaga
      541 tcattgaaga tttcactcat tatgcagaaa cattatttga ggcattccgc gggaaagtcc
      601 attactggat tagtttgaat gaacaaaata tcttcacttc attaggttat ttgttagcag
      661 cacatccgcc aggtgtgaca gacccgaaac gaatgtatga agttaaccat attgctaatt
      721 tagcaaatgc atctgtcatc aataaatttc acgagttaga aatgcctgga aaaatcggac
      781 caagctttgc gtatacgcca aattatccaa ttgacagtaa tcctgaaaat gttctggctg
      841 ctgaaaatgc agaagacctg atggccaatt attggatgga tgtctatatg tggggaaaat
      901 atccgattgc tgccatgagg ttcttagaag aaaaaggatg ggcaccaacg attgaagctg
      961 gcgatgcaga attgctagaa tctgctaaac cagattttct tgggatcaat tactatcaaa
     1021 cggcgacgaa tgcattcaat ccattaaacg gagttggtgc tggaaaaatg aatacgacag
     1081 gaaaaaaagg ttcatcagaa gaaactggag ttcctggaat gtataaaaaa gttgagaatc
     1141 catttgtcga acgaacaaat tgggactggg agattgaccc agaaggatta aggatcggac
     1201 tgcgtcgtat cacaagccgt taccgcatgc cggtactgat tacggaaaat ggcttaggag
     1261 agtatgacaa gttgacagaa gataaacaaa tccatgacga ctatcgaatc aattacttac
     1321 aaagccacgt caaagccatc aaagaagcaa tttcggatgg agcagaagtc ctaggctatt
     1381 gcacctggtc ttatacagat ttgcttagct ggctgaacgg ttatcaaaag cgatatggat
     1441 ttgtatatgt tgatcaagac gaaacacaag aaggatcttt ggaacgatat aagaaagaca
     1501 gttattactg gtaccaaaaa ctgattgaag aaaatggaga gaacctttag ttcacatact
     1561 taaaacaaga aggtacggct ggatatggcc gtgccttttc ttgataaaat catttagaaa
     1621 ttaagaaaaa ctgtaaaata agctgaaaaa tgaaagaaag cgtcagttct ttcaaacaaa
     1681 actttagttc ccttactttt ttttgtataa tgaaacttga tgaaatgagg gataataaat
     1741 ggcacaaaag aaacggccta ctaaaaaaag gaaaaagacg aaaaaaacga aaaagcaaaa
     1801 acagcagcag gaacaaatgc tgacgattgg tgttgggttt gccttgattt tatttgctgt
     1861 cttcggcttt ttaaaattag gttttttagg aatattgatt gcaaacggct ttcggatcat
     1921 tgcaggaaat acttaccaaa tcctctgtct tttattagcc gtattaggat tttggattgt
     1981 cattaagaat acagagtttt caattggtaa aaaccgacgc tggtttggcg gcatactctt
     2041 ttatttcggt atcttattat tgttacatgc ccatttattt ggcaaactcc atactgggga
     2101 accaaatatc atggggacga catgggattt gttagccagt gatataaaac aaagtcaagt
     2161 agacaataac gttggtggtg ggatgattgg agcaatcctt tatcatttta cttacttctt
     2221 gattgcacag cctggaagtt atttagcagc agtgctatta ctggcaggag gagcattcct
     2281 gttcagtaat ctggaaggct atcagctgtt aaatggtatc caatcaattg gagaacgagt
     2341 acaggaatta ttggaaggtg atcctgccaa gcaagctcga aagcaagcgg caaaggaaga
     2401 gagaatgaaa cagcgcgcag aagcgaaaga agcaaggcgc ttagctgcaa aagaagctgc
     2461 agaaaaagaa gctgcagaat atgagaagaa aaaagcggtg agccagcaaa gggaaaatac
     2521 tgctgatgaa tggcaggaac cagaaaatac agagccggag cagctaagtt ttgttccaat
     2581 cgacagtttt caagaaaata tccatcctgc gaatctagag aaaccagtac cagatacacc
     2641 aaaacagacc aatacagcag aaggtttttc agatgagttg ccagaggatg acggtacctc
     2701 attagaattt gagattgaag cagaacagga aaatcaagac tatgaattac cgacagttga
     2761 tttgcttgat tctataccaa ctgtggatca aagtgatgag tataaaaaaa ttgaaaaaaa
     2821 tatcggcgta ttagaacaaa ctttccaaag tttcggtgtc gatgccaagg tcgtcaaagc
     2881 cagcttagga ccatccgtca caaaattcga agtccagccg gctgtagggg taaaagtaag
     2941 taaaattgtg aacttaacag atgatatcgc gcttgcttta gcggcgaaag atgtccggat
     3001 ggaagcaccg attccaggta aatcactgat tggtattgaa gtaccaaatg gaaagatcag
     3061 tatggtttcc tttagagaaa tcatcgaagc acaaccaaac cacccagata aattactaga
     3121 agtaccttta ggacgtgatg tttctggacg ggtacaaacg gctgatcttt cgaagatgcc
     3181 tcacttactc gtggctggtt cgactggtag tgggaagtca gtagcaatta atgggattat
     3241 cacaagtata ttaatgcgtg ctaagcccca tgaagtaaaa ctgatgatga tcgatccgaa
     3301 aatggttgaa ttgaatatgt acaacggcat acctcatctg ttgacgccgg tcgtgacgaa
     3361 ccctagaaaa gcagcgcaag ccttacaaaa agtagtacaa gaaatggaag aacgatatga
     3421 aaaattcgcg gcaaccggag taagaaatat ttctggttac aatgaatttg ttcaacagaa
     3481 aaacttggaa aacggaacga aacatccaac cttaccattc atcgtagtga tcgtagatga
     3541 attagctgat ttgatgatgg tagccagcaa tgaagttgaa gatgcgatta ttcgtttagc
     3601 gcaaatggct cgggcagcgg ggattcatat gatcctagct actcaacgtc caagtgtgga
     3661 tgttatcaca ggaatcatca aagcaaatgt accttccaga atggcatttg cggtatcaag
     3721 cggtacagat tctcggacga tcattgattc taatggggca gaaaagctct tagggcgagg
     3781 agatatgcta ttcttgccaa tgggtgagaa taaaccgatc cgtgtgcaag gagcattcat
     3841 ttctgaccac gaagtagaac gggtcgttca atttgttaca gatcagcaag aagctcatta
     3901 tgaagagaaa atgatgccga cagatgaagt tgaaacgaca ggtgctccag aacagccaca
     3961 agatgagctg tttgaagaag caaaagcact tgtcgttgaa atgcaaacgg caagtatttc
     4021 ccttctccaa agaagatttc ggatcggtta caaccgagca gcacgtttag tagatgaact
     4081 ggaagctcat ggagttgttg gcccgtcaga aggaagcaag cctcggaaag tattcattga
     4141 gcaggaagaa acatcagaaa cgttgccgga cgataatgac atgtaaaact tgtgtgatga
     4201 agggttgaaa aacgaataac caataaaaag acaaggcatg atgataccaa aagaaaaaat
     4261 ggtttttttc catcttttct aatacggtat gatgtcttgt ctttttagtt tttgtgagat
     4321 taaagttcta ttcgatttat ttgtgaagta ttgcattaag gaaatgaatc atgtatagta
     4381 tgtagcgaaa ctttgagaaa aagagggaaa atactttgga aaaacagtcc atttatggct
     4441 tgacgaatga agaattaatc aattggttta tagaaaacgg cgagaaaaaa ttccgagctg
     4501 cccaagtttg ggaatggttg tatcaaaaac gtgtaagtaa tttcacagaa atgacgaatc
     4561 tatcgaaaca attgatcgaa aaattatcgg agcattttat catcaatcct ctaaaacaga
     4621 tggtcgtcca agaagcttcg gatggtacag taaaatattt gtttgaactc cctgataaaa
     4681 acatgattga gactgtactg atgagacaag aatatggctt gtcagtttgt gtgacgacac
     4741 aagtaggatg taatatcggc tgtacgtttt gtgcaagcgg gctgctcaaa aagaaccgtg
     4801 atctgactgc tggagaaatt gttgcacaga tcatgatggt ccaacattat tttgatgagc
     4861 gcaatcttgg cgaacgcgtt tcacatgtcg ttgtaatggg gatcggtgag ccatttgata
     4921 attacgacaa tgtaatggac tttttgcata tcatcaatga tgccaaagga ttggcaattg
     4981 gtgctcggca cattacagtt tccactagtg gattagcaca caaaatcaag gaattcgcta
     5041 ataacggctt gcaagtcaat ctggctattt ccttgcacgc gccgaataat gaggtgcgaa
     5101 catcgatcat gcggatcaac cgaagtttcc caattgagaa attgatggaa gctgtggatg
     5161 agtatttaga gaaaacaaac cgtcgaatca cgtttgaata tatcatgttg aatcaagtaa
     5221 atgatagacc agagcatgcg caacaattag cagatctatt aaaagataag aaaaaattag
     5281 catatgtcaa cttgattcca tataatccag tcagtgaaca tgatcaatat tctcgcagtc
     5341 caaaagctga tgtattgaga ttttatgatg tattgaagaa aaacggcgta aactgtgtga
     5401 tacgtaaaga acatggtaca gatattgatg cagcttgcgg acaattgcga agcaagcaaa
     5461 tgaaaaaaac agttgccaaa taatcgtatc ctactcgttt tttcaattac cataaaagga
     5521 gcagttttct taattaagga actgttcaac ttaaataaga gaaaagattc tatagcgaaa
     5581 gctataaagc tttttttgac aagaaaaacc ctcatcaagc aaagaaatgt cctcaatttg
     5641 atgacagcct gcttgatggg ggttttagca attatttaac tgattttctt atatccttgc
     5701 tggctttctt gtgtagcatg ataagtttct acgtcaagaa tggcctgttt aaatttttct
     5761 cgggtgattt cataaggcac atgtttaata tcatttgttg ccatggcttt atctaagatt
     5821 gcttctaaat cggtcgtgac attgagattc atgtcggtta acgatactgg aagttggttc
     5881 tttttcataa acacatagat tttatcaaac aaatcgaact gctgatccat catcaataag
     5941 gtcagtacac cataactgac agatgctccg tgaaggtatc cctccatttt ttctagaaca
     6001 gtcgtaccgt aatagaatga gtgtgccata gcagaattat agttattgtt aactaaaaca
     6061 gaaaccaatc cggtagtaac aatgatatcc gaaacgactt gttcaattgc atatgaggga
     6121 cgatcgtcgg cactatcttt caatgcttgc gtaccataag tcaagagacg atcaatgcac
     6181 atttttccga tttggattcc taatctatcc gtgtagccta gatcatcgcc acgagcagaa
     6241 aaagtagatt cgacttcttt ggataaagca tcgccaatac ctgcccataa gtatttatca
     6301 ggtgcttcgg caataattgc tgtatcaatg aaggtgtgta caggtggttt tgaaggaaag
     6361 aagagtccgt ccattacgcc ttcttcgtta tagatgacac ataccgcagt gacgggcgaa
     6421 cagttcgaac caatagtggg aaaggtgaag actggtttag ctagtttttc accaatgact
     6481 tttacagtat cgcaagcacg gccaccaccg actgcaaaca ggacgtctgc tttttggaca
     6541 ctttcatgct ggatcaactg ttcaatattt ccataagttg aattaccccc gtaccagaga
     6601 ctggcgacaa cctctatttc ctgtgtcaaa gctactttta ttttgttttc tgcctttgaa
     6661 agtgcagtct tccctccaat cagcactact tttgtcccat attctttgac gattccattt
     6721 gctttttcat acgcatctgt tccaatggta taatttggta agaaaacact ttgactcata
     6781 ttcatttcct tctttcttcg ttgaaacggg ttaaaatttt aaagagtgat cgatctgact
     6841 gcggctgtag atggagtaga ggtgaaccta agacaggata agtgatgaat aaggtggcat
     6901 gaacaccttg aaatcatcaa taagtatcct aaaaacgaac aagtacaaaa acattgaaaa
     6961 cgacaataat gaatttttgt tcctcttagt atttctaccg attccctttg cttttttgta
     7021 attatctaaa aattctaaaa attaagatta ttatatgaaa acaggtttta tttgtcaatt
     7081 cataattggt tttttttaaa gcaaaagata atctatatca tgatgaacaa acagagtttt
     7141 ttcctccaat tgatggcttt cctgatcttt cctattttga ataaagtgtt tctgtataat
     7201 ttttttattt tataagtaag ataaataatg aaagaataga gatactgatt ggtagctaaa
     7261 gatttttgag cgagaacatt tcgttttttc aaaaggactg ttagaataag caagaacaag
     7321 ggggaagacg tatgataatc agtgaatttg accgtaataa tccagtattg aaagatcagc
     7381 tttctgattt actgagactg acttggccgg aagaatatgg agacagctcg gcagaagaag
     7441 tagaagaaat gatgaatcca gaacgaatcg cggtagcagc ggtagaccaa gatgagttag
     7501 taggatttat tggtgcaatc cctcaatacg gtatcacagg ttgggaattg catccattag
     7561 ttgtagaaag ctcccgacga aagaaccaaa taggtactcg attagtcaat tacttagaaa
     7621 aagaagtagc ttccagagga ggaatcacga tttatttagg tacggatgat ttagaccatg
     7681 gaacaacgtt aagtcaaacg gacctgtatg agcatacatt tgataaagtg gcttccatcc
     7741 agaaccttcg tgaacatccg tatgaattct atgaaaaatt aggttataaa atcgtaggtg
     7801 tcttaccaaa tgcaaatggc tgggacaaac cggatatttg gatggcaaaa acgattattc
     7861 ctcgaccaaa ttctcaataa taaaaagagg ttgtgacaga agtaatcagc ttcaagaaat
     7921 aagaaggaat ttacgaaaat tacttcttga atttttgtga aattccaact tatttccgaa
     7981 gaagctactt ttggaacacc gtgtaccaga ggttgtgaaa aagtcgctta gtttcgagta
     8041 gtaagaaaaa ttttgtgaaa atggctttct acatttttgc ataaatttgg cttaataccg
     8101 agaaactggc ttttgaacac cgtttattca gataagggac tgtgacaaga cttttgtcac
     8161 agtttctttt tgtttattct tttttatctt tgtatggcaa taatagacaa tataacgaaa
     8221 attatttaat ggcttttgga agaccatgga aaaccttgta caaagctatt ttttctgaaa
     8281 aattccttga aacctcacga aaaaatcatt attttatgaa aaagacaaaa aaaagtgttg
     8341 attttttaat aattattcat taaactctta aacaacttca gaaaatatta attattctga
     8401 aaattccaat aaaggggtac gtaaaatgaa aaagaaattt acatttggtt tggttgcggt
     8461 atgcggcctt gtacttgctg gatgttatgg cggcagttca gccacaagca gtagttcagc
     8521 aaaaagcggt tcagcagatg gaggaggcgt atttaactta gtcgtgcctc aagaaatgcc
     8581 tacagctgac ttatcagttg caacagatac aatcagtttt acagcattga ataacgttta
     8641 tgaaggaatt taccgtcttg atgaagacag caaaccacag ccggcaggag ctagcgagtt
     8701 ggcagaagtc agtgaagacg gattaactta caaaatcaaa ttacgagaag acgcaaaatg
     8761 gtcaaatggt gatcctgtta cagcagcgga ttacgtttat ggctggcaac gaacagtaga
     8821 cccagctaca gcttctgaat atgcttacct gtatgcgcca gttgaaaatg cagaagacat
     8881 tacagctgga aagaaagata aatcagaact agggatcaaa gcagttggtg actatgaatt
     8941 agaaatcaaa ttgacgaaac aaacaccata tttccaatat ctgcttgctt tcccatcatt
     9001 tttcccacaa aaacaatccg tagtggaaga aaatggggat gcctatgcat ctgcaagtga
     9061 taaagctgtt tataatggac cattcacatt agaagatttt gatggaccag gaacagatac
     9121 agaatggact tataagaaaa atgatcatta ctgggacaaa gatacagtaa aactttctga
     9181 aatcaaagtc agtgtagtaa aagaatcttc tactgcattg aacttattca aagatggcca
     9241 agcagatgac gtgatcttgt ctggtgagtt agcacagcaa aacgcaaacg atccagctta
     9301 tacttctgtt aaagaagcgc ggacaagcta tatcgaattc aatcaacgtg aaaagaattc
     9361 accattcaac aatgtgaacc taagaaaagc tatttcttat tctatcgatc gtgaagcttt
     9421 agtaaaacaa gtactaggtg acggatcagt tgtctctaca ggattgatcc catcagatat
     9481 ggtaaaaagt ccaacaacga acgaagactt tgcaaaagaa gctggaaaat tagtttctta
     9541 tgacaaagat aaagcaaaag aatattggga aaaagcgaaa aaagaattgg gcgttgattc
     9601 tttagaattt gatttgatgg cttctgatga tgattcatcg aaaaaagtaa tcgaatatgt
     9661 tcaaaactca atccaagaaa acttggatgg cgtaacagtc aaaccaacac ctgttccatt
     9721 ctctgtacgt cttgaccgct caacttcggg tgattttgac gcagtactag gcggatgggc
     9781 tgctgactat gcagacccaa gcagctttac tgatttgttt gtcacaggta attcatacaa
     9841 ccgtggtcgc tggagcaatg cagaatatga cgaagcagtt aaagcagctt catcaacaga
     9901 cgctggcgat gaacaagccc gttgggatga tttccaaaaa gcaatcaaga tcatctctga
     9961 tgatatgggt gtcgttccgg tgtaccaaaa agcagaaggt cacctagtga atgaaaaaat
    10021 caaaggtatc gttcaccatg cggcaggcgc ttcatgggat tacaaatgga catacgttga
    10081 agaataatcg ttaataccgt gagcatgaga aaaaacgagg ctgcggcaaa tgtcgcaacc
    10141 tcgttctcat attcacttat cctaacaaag aaaaaagcat gaaaatagca gaaagagcaa
    10201 ttagtattcc ggcaatgatc agccaaaagc tgtacatacc gtagctggca gacgagagcg
    10261 aggaaaattc tggttttttc tttcggttcc ttgttcgtgc aagatggacc gatcgctgaa
    10321 aatgatcgaa gaaacccgaa gaaaagaccc ataaaaaacc gccgataatc agaaacggca
    10381 atgaccataa gaatagatca ttagataatt gaatgagtgt aaaggaatgg ttgatgagat
    10441 tttttattaa aataaccaga atcagtagaa aagctactgc gtaggggata gatttttttt
    10501 tcatttgatt gctcctttat ttttggttca ttatctaata atgtagcgaa acatctgtca
    10561 gaagtcaaaa gctaggggga aacattagtg aatagttata tcaaatatgt attaaaacgt
    10621 gtctttttca tgatcatcac gctttggtta attgcaacaa tcaccttttt cttgatgcaa
    10681 ttgctgccgg gcacgccata tacgaatcag gaacgtttga gtcctgaaac aatcgagatg
    10741 ctaaataaac aagtcggttt ggataaacca gttatcgtac aatatgggat ttatctttct
    10801 aatttgcttc aaggagattt tggaatttcc ttccaattta aaaaccagcc agtcgcacat
    10861 ttgttagctg gaagaatcgg accatccttg cagttagggt tacaggcaat tatctttgga
    10921 acagtcctag ggacgatttt aggaacgatc tctgccatga agcaaaatac atgggcagat
    10981 acatcttcta cactcgtagc gatattaggt cgttccattc caaactttgt atttgctgtt
    11041 ttattacaat atatctttgc aatcaaatta caagttttgc caatcgcaaa atgggatggc
    11101 tttgtgtaca caatcttacc aacaattgcg ttggccatgt cgccgcttgc tgactctgct
    11161 cggtttatcc gtacggaaat ggtggaagta ctgcatagtg attatgtgga attagcaaaa
    11221 gcaaaaggat tgagccgttg ggaaatcgcc tttaaacatg gtcttcgaaa tagtttgatt
    11281 ccattgatga ctttattagg accgttagct gtagcattga tgaccggatc actggtggta
    11341 gaaaatatct ttgctattcc tggtatcggg gaacaattcg ttaagtcaat cacaacaaat
    11401 gattatccta cgatcatggc tgtaacgatc ctttactcat ttatgctgat ttttgttatt
    11461 ttgatcgtgg atctattgta cggtctagtt gatccaagaa ttcgtgtttc agaagggagc
    11521 cgaggataaa tggaaatgat accaaagcgt tatgaaacag ttgcagatat ccctgcggat
    11581 gaatttcttc cattacaaaa aaataccgaa gaagaacgtg aacagattac tgcaccttct
    11641 ttgaatttta tccaagattc atggcgccgt ttaaagaaaa acaaagctgc cgttatttca
    11701 atggttttac tattggtcat cattttgatc tctgtgatca caatctttgt ttctcctcat
    11761 gatccaactg cccaaaacgt ggcttatatc aatttgcctc ctcgtattcc gggaatcaat
    11821 atcgatggat tgaatggaaa agcaatggtt ggtggtgaac ttgttgataa atatgctcaa
    11881 gcaaacgtcc cttcaaacgt aaatttcttt ttaggaacag acggtttggg acgtgatgta
    11941 ttaagccgct tatttatggg aatgagaatc tcgttgctga tcgcatttat tgctgcatta
    12001 ctagatgtaa cgattggtgt agcttacggg ttggtttctg gtttgttagg cggacgtgtc
    12061 gataacgtga tgcaacgttt tttggaaatc ttatcaggaa ttccaaacct cgttgtcatg
    12121 attttgatgt tggtcgtttt tgaaccagga attttttcaa tcgtggctgc tatggccatc
    12181 acaaactgga taccaatggc acgtatcgtt cgtgcacaaa caatgaaatt aaaagatcaa
    12241 gaatatgttt tagctggatt gacattaggt gaatcaagaa tgaaaattgc attcaaacat
    12301 attttgccga atatctctag tgtcattatt attcaaatga tgtttagtat tccatctgct
    12361 attttcttcg aagccttttt aagctttatc ggtttaggtc ttacgcctcc gtcagcttca
    12421 ttaggaacga tgttgagcga agggtacaag acattcctgt atctgcctta tctattatgg
    12481 attccggcag taactttatc ggttatcatg attggcttta acttacttgc tgatggattg
    12541 cgtgatgcct ttgatcctaa aatgaaagag tgaagacgat gacgaaaatt ttagaagtga
    12601 aagacttaga aatctctttt gatacgtatg ctggaaaggt acgagcgatt cgcggtgtca
    12661 attttcattt gaataaagga gaaacattgg cgatcgttgg ggaatctgga agtggaaaat
    12721 cagtgacgac cagaagcatc atgcgcttgc tgtcaagcaa cgccaatata gatgctggtc
    12781 aaatcctctt taaaggtaac gatatcgttg gaaaatcaga aagagagatg caaaaaatcc
    12841 gtggtaaaga aatagcgatg attttccaag atccaatgac ctctttagat ccgacgatgc
    12901 ccattgggaa gcaagtcgct gagtccctta gaaagcataa taaagtttca aaaaaagagg
    12961 gacaaaaagc tgcattagaa ttgcttaaat tagttggaat acccgaagct gagaaacgta
    13021 tcaacagtta tccgcatcag ttctcaggag gtcagcgtca acggatcgtg atcgcgattg
    13081 cactggtatg ttatccggaa atcctgattg ctgatgaacc tacaacagcg cttgatgtga
    13141 ctattcaagc acagattttg gagctgctga aagaaattca gacaaaaatc gatacatcaa
    13201 ttatctttat cactcatgac cttggtgtag tagcgaatgt cgctgatcgt gtcgctgtca
    13261 tgtatggagg aagaatcgtt gaagttggaa catcggaaga aattttctac aacccgcaac
    13321 atccctatac ttggggactg cttggttcaa tgccgacatt agatagtgcg aacgatcgat
    13381 tgtatgctat tcctggttct ccaccggatt tattgaaccc gccaaaagga gacgcattct
    13441 atccaagaaa tgaattcgcg atgaaaatcg atgcagaaca agaaccacca ttttttgaac
    13501 tatcaaaaac acacaaggca gcaacttggc tacttgctcc gcaggcaccc aaagtgacac
    13561 cgccagaaga aatccaacgt cgctgggcta tctttaaaga gaaacaaaaa catacttacg
    13621 gtcaaggagg aattgctgat gccaaataaa ctacttgaag ttaaaggatt gaaacagtat
    13681 ttcaatgtag gaaaaaagaa tgaagtacat gctgtaaatg acatcagctt tcacatttat
    13741 gaaggagaaa cattcggttt agttggagaa tctggaagcg gaaaatccac tactggccgt
    13801 acgatcattc gcttgaatga acctactgat ggtgagatat tattcgacgg tcaagatata
    13861 acgaagatca aagataaaaa aggtttgacg aaattccggc atgatgttca gatgatcttt
    13921 caagatccat acgcttcgtt gaatcctcgt atgaaagtca gagatatcat tgccgaagga
    13981 attgatgtta atggactagc taaatcatct gaagagcgtg caaaaaaagt cgatgatcta
    14041 ttgagaactg ttggattgaa tccaagccac ggtactcgtt atcctcatga attttcaggt
    14101 gggcaacgcc agcgtattgg tattgcacga gcattggcag taaaaccgcg ttttatcatt
    14161 tgtgatgaac caatttctgc actagatgtg tcgatccaag cccaagtggt caatttgttg
    14221 caagatctcc aaaaagaaca tcaattgacc tatctattta ttgcacatga tttgtcgatg
    14281 gttaaacata ttagtgaccg aatcggtgta atgcataacg gcttgttatt agagatggga
    14341 acaagtgatg aaatctataa tcacggtgtt catccttata cagaaagttt gttatctgct
    14401 attcctctac cagatccaga ccatgagaga aaacgcagaa gaatcaaata tcaacctgaa
    14461 ccagatgacg gacaagtacg tcaacttcgt gaaatcgcac cagaacattt tgtctatgca
    14521 actgagcagg aagtcccata ttacgcaaaa aaactaaaac gacaaaagga atctcttctg
    14581 gttgctaact aggagggaca tagatgctaa aagcttttaa attccgtatg tatccgacag
    14641 aagaacaaaa acagcaattg attcgtactt ttggatgtgc tcgatttacc tataaccatt
    14701 tgctgaaaaa gcggcagaaa agttggcaac aaacaggagt agccaatttt tcgctcacac
    14761 ccgctacatt aaaaaaagaa tacccattct taaaagaagt agacagtctt gctttagcaa
    14821 atgcacagct taatttggat cgggcttttc gcaattattt caagggacgt gccagcttcc
    14881 caaagcttaa aacgaaaaaa agtatgtggc aatcttatac gaccaacaac caaacccgta
    14941 cgatctatct ggaaaatggc tacttaaagt taccaaaaca aaaagaacta atcaagataa
    15001 atcagcatcg gccagttgaa ggatcgattc gctccgctac gatttctgct cgatataatg
    15061 aagaatttta tgtcgcacta ctttgtgatg tttcgccagt aaaaaaagaa tctttagcta
    15121 aatggatcgg gatcgcctat catccgaaga cattgatcca aacatctcga cctctagaag
    15181 taactttacc aaaatttcac cagacagaag aaaagctgca acatgcacag cggaaattga
    15241 atgtgaaagt ccgctccgct caccatagaa agatcaggtt agatcaggca agcaattatc
    15301 agaaacaaaa gagaaaagta atggatcttt atctgaaaca aaagaaccag cgagaagatt
    15361 atcttgaaca attatctggg aaattggtca aacaatatga ctacttattt gtagaatctt
    15421 ttccaaaaga agaggcacat gctgattttt ctattcacga ctggcataaa ttaataacaa
    15481 aattgcgtta taagtcccaa tggtataata aaaaatttct tttgatcaat accgacggag
    15541 cagaagaaag caattccgta agaaagagcc aagtagtaga aaaaatgggc agacattcgg
    15601 ttatcaaggg ataaaaaatg aatgaaactg ataggagcgt tagggttagc cttgttaata
    15661 aaaagtctta atgacttttg tttctaggaa tttttctttt gaagaaaagc tcactaatta
    15721 tgacaaacca ataaaacaat agcagaaaaa gttcgtacga gctttttctg ctattgttaa
    15781 ttttttattc agaaagttta cgaatacatc aaaattctga aacaaaaaac gtaactcaca
    15841 aagagttgtt tttgcgtttt ttcttgaatg attaggtctg aatcttttgc tcacaacctt
    15901 attcatggtg ccccggcttg agctgataga tttttgaagg acggccgatt cctacaggac
    15961 gttcaccgat ctcatcgaaa tattggagca tagctttctt aaaattggaa tgatcgatcg
    16021 ttttataatc gatccctaag aatttagcaa atacttttcg agcttccgta atcgtgaaat
    16081 ctttacctaa aacttgtaga acttgcggct cgtgttccat tttattgact acacggttga
    16141 atgctttcaa gatgatttga ctatggtcaa aagccaaagt atctttgcca gaagactctc
    16201 cagtgactaa atcaagcgag atagccactt ctccgctaga taaatggatc ttgttactat
    16261 gacgttctag tgtgaaccag cgtacttcct tggcatcatc tccggcgatt agcggttctt
    16321 ctccaataaa ggctaagtag ctgactgtga cgacccagcc tcgcggatct cgattaggcg
    16381 tactgaatgt atgcagttgc tcaatatttt cttttgaaat aaccacacca gtttcttcct
    16441 ttgtttctct taagacgctt tctccagttg actcatcctt ttggacaaag ccaccaggca
    16501 atgcccatga atttcgataa ggatgccctt ttctttggat caacaaaact ttcagctgat
    16561 cttcttcttt gttatagcac atcagtacga tatcgacagt caaagagggc ttgtcatatt
    16621 ctggacgttc ctgtttttga taccagttaa gaaattcttc ttcacttgct ttatgttcat
    16681 aatataattt ttcttcttct ttagaagcaa aacggttcat ctgaccacct ccattgacag
    16741 taaaagtact ttttacctta ttgtagcaga ctctgaaaaa aaaccaagga tttacttgaa
    16801 aaatctaggt tatcatttaa atatgaaaaa taaatggaag tatgagattg aataaaaaga
    16861 aaagcagata aaacggcaat actttatacc gctcttcacg aatggcaaag aagccacaaa
    16921 taacgatata tacgtcaatg ataaaacgca aaatccaagc tgctgtcata aaactgttca
    16981 aatgcccatg gaggttaaaa taaccataca tacgaatata aaaatagctg aatattaaat
    17041 gtgctaatga aagaaaagca atactaagtg taaaaaaaag atgtttcttc tgcatgattg
    17101 ttccagtccc ttactaagat agatagtctt tattatacca cgtgtgaggg gagaaataaa
    17161 aagccgtttt ttagtagata tgaaacaaaa caaaaacccg catttattca acctattcat
    17221 ccttttaaag gatagattga acgtagatag aagtcgttta tccagtcttt ttcattttga
    17281 aaaaaagcta acagcatgct ttttttatgg tatactagca aggaaacgaa ttcgcatcat
    17341 gcgataaagg aggaagagcc ccgtggcaga aaaggaaact ttttatatca ctacgccaat
    17401 ctattatcca agcggcaaac ttcatatcgg caactcgtat actacgattg catgtgacgc
    17461 aattgctcgt tataaacggt tgatgggctt tgatgtgttt tatctcactg gagtggatga
    17521 acatggtcaa aaaatcgaaa caaaagcaga agaactaggt gtaaaaccgc aagaatatgt
    17581 agataaaatg gcggctgatg tcaagaaatt atggaaaacg cttgatatca gttatgataa
    17641 attcattcgc acgacagatg attaccataa agcagctgtt caaaaaatat ttgatcgttt
    17701 attagaacag ggagatattt acttaggtga atatgaaggc tggtactctg tttctgacga
    17761 agaattcttt acagaaacac aacttgcaga agtgtacaaa gatgacgaag gtaacgtgat
    17821 cggtggtaaa gcgccaagtg gacacgaagt agaattggta aaagaagaat catacttctt
    17881 ccgcatgagc aaatacgcag accgtctatt ggaatactat gaagaacatc ccgaattcat
    17941 ccagccagaa tctcgtaaaa acgaaatgat caacaacttt atcaaaccag gattagagga
    18001 tcttgcagtt tcacgaacaa cttttacttg gggagtacca gtcaagagtg atccgaaaca
    18061 tgtggtgtat gtatggatcg atgcattatc caattatatt actgcactag gatatggctc
    18121 cgaagatgaa agtctattcg aaaaatactg gcctgcagat gttcagatgg tcggtaaaga
    18181 aatcgttcgc tttcatacga tctattggcc aatcatgctg atggcccttg atttgcctct
    18241 acctaaaaaa gtatttgggc atggctggct attgatgaaa gacggaaaaa tgtctaaatc
    18301 aaaaggaaat gtcgtttatc ctgaaatgct tgtggaacgt tatggtttag atgcactccg
    18361 ttattatttg cttcgtgctg ttccgtttgg gtcagatggt gtctttacgc cagaagattt
    18421 tgtttcccgt gtcaattatg atctggcaaa tgatctaggg aacttattga atcgaacgat
    18481 tgctatgatc aacaaatatt gtgatggact cgttcctgac tatgcctcac ttgtcactcc
    18541 atttgatagt gaactgtcta cgacggctgc taacgttgtc ggacgttacc atgatgcaat
    18601 ggaaaaaatg gaattcaata cagcattggc tgaaatctgg gtgctgattt ctcgtgcaaa
    18661 caaatacatt gatgaaacag aaccatgggt attagcaaaa gacgaagaaa agaaagctga
    18721 attgaatagt gtcatgatcc atttagctga atctttgcgt atcgcagcga tattacttca
    18781 gccaatcatg acagaaacgc cagagaagat cttcaatcaa ctaggattgg actctgaaac
    18841 aatgaatctt gaaggattac actttggcga atttccttca ggaacaaaag tagttgcaaa
    18901 aggtacacca atcttcccac gtttagatat ggaagaagaa gttgctttta ttcaagaaaa
    18961 aatgtctgaa ggtactcaaa caaacgaaga tacagtcaaa tgggatccag aagaaacaga
    19021 acttgtttca acgaaagaaa aacaaatcaa atttgatgta tttgaaaaag tagaactaaa
    19081 agtagcagaa gtcatcaact gtcaaaaagt agaaggggca gataagttgc tgcaattccg
    19141 actagatgct ggagacagcc aagaccgtca aatcttgtca ggaatcgctg aattttatcc
    19201 tgatccaagc gaattgatcg ggaaaaaagt cgtcattgta gcaaatctga aaccacgaaa
    19261 gatgcgtgga caaatcagtc aaggaatgat cctttctgca gaagcaccag atggctcttt
    19321 acaagtaatc gaagcaccaa aatccatgcc aaatggctca gaaatcgcat agaaattcaa
    19381 agggggtatg acactagttg tgtcataccc cctttttccg aaatttgaga agcaattttc
    19441 gtaaattcct tcttattact tgaagccaat catataatac agaaacagag aactatccaa
    19501 aataaatcct agaaaaagaa gtacgagctt cgctgatttt tgttaattta gaataatact
    19561 aataggaatc gtaaaaacag aaaaaaagca taatgataaa gggtcgggca gattttaatt
    19621 gattcatctg gtactctttt ttattgtcct tacattcata aagtttgaat gcaatgactt
    19681 tattacatct atatttatat ctaacagtag cgttgatgtg ttaattataa gttctatcac
    19741 tttatgttca attagtttgt gctcttatat gtgtgattga aaatatagat aaaaaaacaa
    19801 tcatcggctt tgacttcgat gattgttttc tttattttgt aaaaatccta tcacttgatt
    19861 ttatggtaac cttttatttt tgtcttcaaa cggcttggta agagagcagt aagcagtgca
    19921 aatgggatcg aaaagacata ccagaaaatc gtacgtccga taccataatg ttgatagagc
    19981 tttgtcaaca tcacgtgtaa caagatcaac cctaaaagca aggagccagc tataaaccaa
    20041 tgattatccc aatgcatgac ttcactaagt gccatcactc caaaaggata gaaataaaaa
    20101 tagatcccga ctaaggtcat ccgccagttc tttgtaaatg ttccgatatt caataaaaag
    20161 aaaatcgctg aaagcaaaat accgataggc ggaaaaagcc caaccaaaaa ggaatacaac
    20221 atcccccaca agatcatcac aggacctttg atcaatgcaa cgattgcgat gaacaaagtc
    20281 agcaatatta gtttttgtga taacgttgca acagtcatta atccaaataa aattaatgtg
    20341 aaccaatgcc aatgggactg ctgttgttct ttacgggaaa aatcgacccg gttggataaa
    20401 taatctgcta atttcggata agaacgctta aaatccataa actgttcctc cttcgtggaa
    20461 aaggtaggtt tagtatacga aaaataaaaa agaaatacat ccgtcttaag agggagtttt
    20521 tcactgaaag tggagaaaaa agaatggtaa attaaaatat tctcagttat actgaaaatt
    20581 ataccaaatt tcaaagaaga aagaggatgg gaatgaaagg caaactaaaa cgagaaatca
    20641 atctgtttgg cgcattagct accgttatgg gaaccgtgat cggtgcaggc gtcttcttca
    20701 aaactgctgc tgttactgct agtactcagt ctacaagttt gacattgcta gcttggcttt
    20761 tgggtggttt cttaacgatc tgtgctggat tgacagtggc tgaattagct acagcaatcc
    20821 cggaaactgg tggagctgta aaatatattg aagctgcgta tggaaaattg cccagctttt
    20881 tattaggatg ggcgcaaagc ttgatttact ttcctgctaa tattgctgca ttatcgatta
    20941 tctttgcgac acaattgacg aacttattac aattatctac cgattatctt ttgttgattg
    21001 cagtgatcac cgctgtttct gtgacagggt tgaatttgtt agggacaaaa gtcggtgctt
    21061 ctgtacaatc agtgacgttg attgttaaat taataccaat tgctgtgatt gtcatatggg
    21121 gattattgac cccaggacaa ggaactgtcc agttatttcc aatcgaagct ggaaaagatg
    21181 ttacttttgt cgaaggattg agcagtgcgt tgttagcaac gttgtttgct tatgacggct
    21241 ggttaggtgt cggtgcaatg gcaggggaaa tgaaacggcc ggaaaaagat ttgcctaaag
    21301 caattattct tggtttgagt tttgtaacag tcgtttatct attgatcaat tttgtttttt
    21361 tgaaaacttt gccgattgat catcttgccg gtaatttgaa tgcagcttct gaagcatcag
    21421 acgtcatttt tggaggaatc ggtggaaagc tggtcactat tgggatcttg atctcggtat
    21481 acggtgcatt gaacggttat acactaactg ggatccgcgt gccttatgcg atggctttag
    21541 aagacgagct acctttcagt aaacagctga caaagctttc aaagaagttt acggttcctt
    21601 atgttcctgc ggttttccag ttagcagtag cttgtattat gatgagcctt ggttctttcg
    21661 actttttaac agacatgttg atttttgtga tgtggctatt cagtttactg atttttatcg
    21721 gtgtctttat tttaagaaaa aaagcgccgg aattgccacg tccatataaa gtaccacttt
    21781 atccaatcgt tccgattatt gcgattctcg gtgctatttt cattttaggt atgacgatga
    21841 tgacgcaaac gaaattagca ttgatcggta taggtgttac cttgataggt atcccagtct
    21901 attatcaaaa aaagaaaaaa cgctcagaag agtaaaatat ttcaacaaca gacagaaaaa
    21961 gtacgaacta ggtcctctag atcttcgtgc tttttctatt tcaataaaac aagttcttct
    22021 attttgattt ttgctacttc tgcaagtagt gcttatgtgc taaagtaaga atgaaaagga
    22081 gagataaaca tgatatttga ttctcatacc catttaaatg ctgaacagtt caatgaagat
    22141 attccagaaa cgattcagcg agcacaagaa atgggggtaa cgaaaatggc ggtcgtgggc
    22201 ttcgatacgc caacgattga aaagtctttg caattaagcc atgactatcc aaatatatac
    22261 agtattatcg gctggcatcc gacagaggct ggcagttata cgaaagaaat cgaaaataaa
    22321 ttacaagaac aattgacttt accaaaggtt gtagcattag gggaaattgg cttggattat
    22381 cattggatgg aagacccgaa agaagtccaa gataaagtat ttcgcagaca gattgctatc
    22441 gcaaaagaaa tgaacctacc aatcagtatt catactagag aagcacttga agacacgtat
    22501 caaatcctaa aagaagaaga tgttcgagat atcggtggaa tcatgcatag tttcagtggt
    22561 gattatgaat gggcaaaacg atttcttgac ttagggatgc atatttcatt tagcggtgtc
    22621 gtcactttca aaaaagcatt agacgtccaa gaggctgcag caaatgtacc aatcgaacga
    22681 ttacttgtgg aaacagatgc cccttatctg gcaccagttc cttttagagg aaagcgaaat
    22741 gaaccaggct atactcgtta tacagtagaa aagattgctg aacttcggaa tcttccgaca
    22801 gaagaagtgg ctgcccaaac atggacaaat gcgcaccgat tatttggtct aagcgaacat
    22861 ggataagatg aagatccaag agattatagt cgttgaagga aaagacgata caagaaggct
    22921 gcgggaagta gttgatgcag atacgataga aacaataggg tctgcaatca atgaagagat
    22981 cctgatgcaa attgaacatg cccaagagac tcgtggagtg atcgttttta ccgatcctga
    23041 tttttcagga gaaaaaataa gaaaaatcat tatggaagct gttcctcagg caaaacatgc
    23101 ttttttgccg cgcacacaag ctaaaccaaa gaaaaaggga agccttggtg tcgagcatgc
    23161 aagtgatgct gctattttag aagcattgaa aaaggtcgtg acgccagaca tttcccaaac
    23221 ggctgtttct gaaatcacta gacaagacct tttagactat ggtttaatag ctggggcaca
    23281 agcaaaagaa aaacgagaaa aactaggtga cgagttgcgc atcggttaca cgaacggcaa
    23341 acaattggaa aaacgtttga agatgtttcg tattacgccg aaagaactcg cccaagcaat
    23401 gaaaatagtg gaggaacaac atgagtgaat atcgtgaaat tgcgacaccc tctagaacaa
    23461 aagagatttt attgaaacat ggtttttcat ttaaaaaaag tttaggacaa aattttctga
    23521 ctgaaccaaa tattttaagg aaaatcgtag aaactgcgca aatagatgaa catacgaacg
    23581 tgatcgaagt tggaccagga atcggtgcat tgacggaaca attagccaag catgcaaaac
    23641 aagtcgtagc atttgagatc gatgatcgtt tgatccctgt attggctgat acgatgcaac
    23701 cctatgacaa tgtgactatt gtacatcaag atattttaaa gaccgatttg tcaacagctg
    23761 ttagagaaac atttcatgag gagcttccgt taaaagtcgt agcgaattta ccttactata
    23821 ttacgacacc gatcatgatg cattttcttg aatcagattt ggttgtggat gaactagtcg
    23881 tcatgatgca aaaagaagta gctgatcgta tctctgctga accaggaacg aaagcttatg
    23941 gttcattatc gattgctgtt caatattata tggaagccag tttagcattc atcgtaccaa
    24001 aaacagtatt tgtccctcag ccaaatgtag attcagcgat tttgaaactg acaagaagag
    24061 atataccggc agtcgaagtg acagacgaaa aagcattttt ccgattgaca aaagcggcat
    24121 ttcagcaaag aagaaaaacg ctttggaata acttgcagca ttcgtatggc aaagatgacc
    24181 agacaaaagc atggttagca aaaagcttgg aaacagctgg aatcgatcca aaacgtcgtg
    24241 gggagacttt gtcattacaa gaattcgccg cattaagcaa tgcgatgtca gaaaatatgc
    24301 agtaaaaaaa gaggttgtga cagaagttgt gaaaaagctg tcctgcatca agtaataaga
    24361 acagccaaac aaaaatagtt cctcatgttt ttcgtttgtt tgggcttgtt accgcagatg
    24421 caagcttttg aacaccgttt aagaaagagg ttgtgggcct gccgtttaac tctaagtcac
    24481 aaggaacaat ttcaaaaaac agcttctcat gtttttgagt tagttggtct tattacttga
    24541 tgcaagcttt tgaccactat tatgccgaac aaggagatgt gacacaaatt ttgtcacatc
    24601 tcctattttt gtttcttgat caaaaaattc ttatacttga tactcacact cattttgtat
    24661 ggatttagaa aagaatatgt gtcttgacag ttattagtaa gtgagttata ctaagtcgac
    24721 atttatgtgt caagacatat taggagagag aaaaaatgag aaaaaattca gttcgaaaac
    24781 taacgatttc tgctttattg attgggatgg gggtcattat cccaatggtc atgcctaaaa
    24841 tcatgattcc accagcttcc tttactttgg cgtctcatgt gccattattt attgcgatgt
    24901 tttttacacc aggagttgca gtggctgttg cgctaggtac gacatttggt ttctttttaa
    24961 caacgccggt tatcatcgca ttgcgagcat tgtcacatct tgtgtttgcg ttgattgggg
    25021 cttggtactt gcaaaagcat ccgagtatcg ttttaaaaaa tggacagttt acttttgcta
    25081 attggcgttt ccaagggttc aattttgtga tcggtgtgat tcattcttta gcagaaatgc
    25141 tagtagtcag catcttttac tttattggta atatgcctga aacatattat tctcaaggat
    25201 atttttatac agtattcatc ttgatgggga ttggtgggct tatccacagt ttagttgatt
    25261 ataatattgc ttattttata gcaggaacac tcagcaaata ttttgatatc cccgtattta
    25321 cggcagcaaa aaaagaaaat cggaaagagt cgattcaagg gtacgcgaag aaaacgacat
    25381 attagacatg atctctcaaa aaaaaaactc tcatctagta atggataata aatcctctaa
    25441 gatgagagtt ttttgtccta aaaagagctg caccaacatc ttctggcaca gctctaacag
    25501 ggggcaattt atggaagttt gatgacttgt tctgtttctt tatcaaagaa gtggcctttg
    25561 ctgatgttga aggcaaggtc gactatttcg cctggtttat ggaaatctct tgcatctact
    25621 tttgagatga actcagtaga accagttttt gtgtaaagca tcgtttcagc tccaagtaat
    25681 tcggaaacga caacttctga tttcactacg gcttcaggtg aagcttcaag ggctacttgt
    25741 tcactgtgga tgtcttctgg acgaataccg aagataactt cttttccttc ataacctttt
    25801 tcaactaata ctttgttttt tccttcagga atacgaagct taagcccatg tccatctgtg
    25861 ataacaccat ttttcaatgt cacattaaag aagttcatgg caggagatcc aataaagccg
    25921 gcaacgaaca tattgacggg tgtatcataa acttctttcg gtgtaccgat ttgttggata
    25981 aatccgtctt tcatgataac gattcggtca gccatagtca ttgcttctgt ctgatcgtga
    26041 gtaacataga tggtcgttgt ttcaagacgc tggtgaagtt tggcaatctc agcacgcatg
    26101 gctacacgta atttagcatc taaatttgat aatggctcat ccattaagaa gacttttgca
    26161 tcccgaacga ttgcccgtcc caatgccaca cgttggcgtt gtccaccaga taaagcagct
    26221 ggttttcgtt tcaagtattc tgtcagacct aagatctctg ctgcattgtc cacacgtttt
    26281 ttgatgtctg ctttatcata tttacgtaat ttcaagccaa aagccatatt atcaaaaaca
    26341 gtcatatgag gatacaatgc gtagttttgg aaaaccatgg caatatcacg gtctttaggt
    26401 gccacgtcat tcatgacttt tccgccgata tttagttctc cttcagagat atcttccagt
    26461 cccgcgatca ttcgcaaagt agttgacttt ccacaaccgg aaggaccaac aaaaacgata
    26521 aattcacggt ctttgattga tagattaaaa tcagttacag aataattttc tgcattgtca
    26581 tattttttat atacgttttt cagagccatt tctaccataa tacgttccac actttctttt
    26641 ttagttttat taactattta aagtataaat gaaagcgtta actaacttca atgtcatgtt
    26701 gaacaagaat tttttctttt ttttgtcaat gtgctagttt taagctttaa taaatatcca
    26761 taagaaataa aaactgatac actggagtaa ataagtatgt taacaatatc aaaaggagtg
    26821 tctcttatga aaatcgcttt gatcgctcat gaccgaaaaa aacctatgat ggtgaaatta
    26881 gcaacagcgt ataaaacaat attggaaaaa catgaacttt ttgctaccgg gacgactggg
    26941 cagaggatca cggaagcaac cggcttgccg atccatcggt ttaaatccgg accgttaggc
    27001 ggggatcagc aaattggagc catgatttcg gaggatcagt tagatttggt gatttttctt
    27061 cgagacccgc tggctgcaca gccccatgaa ccagatgtaa cagcacttat ccgtttatct
    27121 gatgtctatg agattccgtt agctacgaat atcggaacag ctgaggtgct actaagagga
    27181 ctggaagccg gcttcctaga ttttcgaaga atcgtccatg aaatagacaa gcatccactt
    27241 tcattttgat ggataagcgt atggaaaaat aacgtttaat gttcgcctat atgtagctat
    27301 aaaagctaaa caaactgata atatgcgata taatacgaac gtattggata atataaacaa
    27361 taatattagt ttttcgtatt tgcagtaaat gtgaaaatat gctatagttt ctgtgggaaa
    27421 agatcccaaa aataatacag agtagaaaat aaagcgttaa gtgctaagag gatgggatgt
    27481 tgcctcttgg acgaaggacg taatgttcgc ggttttattt tcgcattcgc tgcatgatac
    27541 gatgtagcag ctcttaaagg agatgctaaa aatgaagaaa caccggttag acgcacgtat
    27601 gattgctatt atgggcttat tgattgcatt gatggtcact ttgtcccgac tggtagcaat
    27661 cgagacccca tttattaaaa tcagtgtcac atttattcca caagtgatca tgggcattct
    27721 ttttggtcct ttttggtcag ggataggcgc agttttagct gatttagtag gaatggccct
    27781 cttctcgaaa tctgcattct ttattggatt tacattgaat gcatttattg aaggagcaat
    27841 ctacggattc tttttctatc gaaaagagat cacttggaaa aatgccattc tggctacgct
    27901 tagtgtcacg ctgatcatca acttattttt gacaccatta tggcttgcgt tgatgtacca
    27961 tgtgccattg ttcagctggg tcgtttgggc gccccgcctg ttaaaaacag taatatggtt
    28021 accgattcaa tcaattgcca tttattacgt aggacgtagt attccttaca aaaaaatttt
    28081 aagaagttta gcgatccacg ctaaataaaa aacaagcagt cttcccatcc cagaagactg
    28141 cttgttttta ttgtgctgtg aattgtgctt gatagagccg tcggtaataa ccatcttttt
    28201 gaagcagctc atcatgggtg cccatttcga caatagctcc ctgatccatc actaatatct
    28261 ggtccgcatt tttgattgtg gacaggcgat gggcaatcac aaaactggtt tttccttcca
    28321 tcattgctaa gaaggcttgt tggatttttt tctctgtcaa tgtatcgact gagctggtag
    28381 cttcatctaa gatcaacata gggggattac tgatcatcgt tcgagcaatc gtcaacagtt
    28441 ggcgttgtcc atctgatatc tttaaccctt gaccgccaat cttagtatct aatttttccg
    28501 gcaaacgttc tacaaattca tacatataag aggcttttag ggcatcattg atttctgcgt
    28561 cacttgcttc cggattccca aaacgaagat tttctcgcaa tgtactgtca aatagccaag
    28621 tatcttgtaa caccattcca aaacttttac gtaatgtatc acgttggata gagcggatgt
    28681 catggtcatc gactgtaatt gttccttttg ttgtgtcata aaaacgcatc agcaaattaa
    28741 caagtgtgga ttttccggcc cctgtttttc ctactatagc aatggtttga cctggataag
    28801 cagtaaaatt gaaatcttcg atcaaacgct gcttggggtc ataagaaaaa gaaacatgat
    28861 caaaagagac ttttcctttg atatttttgg gttccaaggc ataggcatct ggcaaatcag
    28921 gcttttcttc tggctgatcg atcaaatcaa atgctcgttc tagaccagaa aaagcggttt
    28981 gcagttgtgt cgtgattcca gaaagttcga taaatggttt agaaaactga cttgagtaga
    29041 tcgtaaagct ggaaatcaca ccaacagtga ttgacgaacc actcttcaat gccaataggc
    29101 cgccaactaa accaatcgat aaataagcaa ggtgatctac aaaacgagaa gacgggtttg
    29161 tcaaggaaga agaaaattgt gcccgttgtc ctttttcata tagatcagca tttagcagtt
    29221 caaattgctg ttgactagct tcttcttgct gaaaagcttt cacgattttc tggttaccga
    29281 ttcgttcggt cacaaagccc gagatatccc caataatcct ttgctgtcta tcgaaatttg
    29341 tttgtgaagc acgagcaacg atccaactca caaggaaaat aattggtgta gaacaaagaa
    29401 cgaccaatgt cagaagtgga cttagacgca tcataaaaat aaacgaaaga atgatgattg
    29461 ccactccaga aaataattga ttgaaggccg cagaaattgc aatggaaata ttgtccatat
    29521 catttgtaaa tcgactgacg atatttccgt gggagttttg atcataataa cgaagtggca
    29581 atcgattcag atgagcaaat gcatcttttc ttaattttgc aacggataag taagctaacc
    29641 gattgcttaa cacttggatg agccattgac tgactacgtt gattaaaacg ataccaccga
    29701 atagaataag taaatggaac agtcgattga agcggacctg tccttctcca atcatttgat
    29761 cgatactttg accaatttgt aacgtcatca caactgttgc aatcccattg atgattccta
    29821 gtatcacagc tagccaaatg tcttttgcat aggcagtaag gtaaggaatg aatcggcgga
    29881 gcgtctgcaa ggataatttt tttactttca taactattct tcctcctgtg atgcgacgat
    29941 ttcctgatat tctgctgatt gagcaagtaa ttcttcatgg gtaccctgag cagcaatctt
    30001 accttgatcc atcactaaga tatgatgtgc ttcttgaata gagcggacac gttgggagat
    30061 aataatcact gtactgccaa gataagtctg tagcgcctta cgcaaatcca agtctgtttg
    30121 ataatccaac gcacttaaag agtcatctaa aatcagtaat gctggcttgg caatcaaagc
    30181 tcttgcaatc gtcagtcgct gtttttgtcc gccagagaaa tttttcccat tttccatgac
    30241 ttttgtatct aaaccatttg gaagctgacg gacaaattct tctgcctgag cgattttcag
    30301 agcttcccaa cattcttttt ctgtggcgtc tttcttaccc cattgaaggt tttcacgaat
    30361 agtcccggta aatagtacag aagtttgggg aacaagagca atttgctgac ggagatgatc
    30421 cagctgccaa gaacgaacat ccattccgtc cattataact gtaccggaac tgacatcata
    30481 gaaacgcgga atcaattgtg tcaaagtcgt tttcccgctt ccggttggac caatgattcc
    30541 aaggactgta tttcgcttca gctgaaatgt aatattttgg agagaaagac cagaatcagg
    30601 agtataacgg aagtcaacat ggtcaaaagc gataattgct tcagaagaaa aatcgggagt
    30661 agtactgacc tcagcttcaa gaatcgaatt ttctgtgtct agcacctctt tcacccgatt
    30721 tcctgaagct tcggcacgtg tgaagatcac gacgaggttt gaaacaacga tcagtgccaa
    30781 aagcatttga ttcatataat tgatcaaagc aagaacttgt ccttgttgca agctaccaat
    30841 atttactttg atcccgccaa ctgtcagcaa acagatgatc ccgacattca tgatcaaggt
    30901 tgtagcaggt gataataggg cagagatatt ggaaacacgt aaataattgt tcgctagatc
    30961 atttgtgctt ttatcgaact tctctgtttc tttttttgtc tgcgcaaatg cgcggatgac
    31021 acgaacaccg ctaagattct gactaacgtg ttcattgagc tgatctagtt tttcttgcac
    31081 tttttggtag agaggaacgg tttttttgat gataaaaaat aaaatgattg agaaaatagg
    31141 caataatgct aagaaaaaca gacctacttc ccagtcgatc acaaaagcca tgaccactga
    31201 cccgatactt aaaaacggtg ctctgatcaa taaacgaatg accattgcta aagctagctg
    31261 aagctgattg atgtcgtttg tcattcgggt gatcaaggta tctgttccaa aatgattcaa
    31321 ctcgttgtga gagaaagtat tgattttttt gattagctga ttacgtaact ctgtaccaaa
    31381 tccttgtgaa gctactgaag catagtattg gcaaatgata gcacaaatca gaccaatcac
    31441 agacatagcg accatccata agcccatttc aatgattttt cctcggtcat tcatttttag
    31501 tccattatcg atcaaggaag ccatcattaa aggcaggaca agttcgaaga cagcttcaag
    31561 aaatttgaag aacgggccca aaatgatttg cttacgatag tcttttgcat aacgtaataa
    31621 ctgaatcata tgattcctcc tattatttat ttgtccacaa ttgaaaaaac aagtactacc
    31681 acattgtagc ttaggaaatt tagaatgaaa agagaacaag ccttttcata aatgaaagtt
    31741 ttattatgaa gaagctttag aaattgaata gactattttt tttaaaaaaa agaaaatgag
    31801 aaaacatgcc agtctcttca ttcataggaa cgtctatttt acttaatttt tctataaatg
    31861 aattctgcat aaaaaatata tcaagtaaat gtttatagat gaaactctat ttacttcttt
    31921 tttaatttgt tcttgcaatc ttttttgcta cgtaattttt gtaaaaaagt gggttgagag
    31981 aaatctaatt gattttcaaa gaaggagtat gaaaatgaga aaaatattta ttcgtttatt
    32041 gtgggtacta tcttagagga ataaataata gtggagaaaa ctatggtttg gcgcagattc
    32101 tatcaggttt gatggaacga agacaacctc ccgacatcaa gtcaaaagct acttgagatt
    32161 ttgatctgaa aataaaaaaa gaaaacaagg taggaacatc aatgtttcta ccttgttttc
    32221 gtaatcaatg ggaaaaaact aagagtggtg tacgtaatca ataaaaagga tggtgacaac
    32281 agtacctctt tactctctga aagcatcata actcgttttt cctcattatg ccaataaatt
    32341 gttgttatac cacatataag gatttgtaat agctaaaaac agacaggaaa gttgaaatct
    32401 gtgaatctta ttttgtatgg aaggatttat agaacgcaaa aatcaagata aagatgaata
    32461 aaaatcctgt ttcggctata tattgcgtat ctacgccaat aaaacgagtt tgttctgtca
    32521 tagccatata aagcaaaggc aaaaatacaa ctacagcgag ataagatccg atgatttctc
    32581 gaaaagtttt ccaggaagta tgtttcctgt ttttatattt atccgatttc attgaatagt
    32641 catagcctcc ttattctttc atatgttgaa attccagtat aaaaactgga aggaactgta
    32701 cgttttttag aataaaaatt ataagggata agtaggctgc tgtctataat aagatatatt
    32761 tttcaattct ttattaaaga ttttattgac aaaatgaatc cgttttctat gatggaggag
    32821 gggtgaataa gttgttgaag aaggaaattt tatgttactt agagaaccag acagcctttt
    32881 tcgatttaga acatatgaac gaggtcttta cagcacaaag gttagctgat cattttgggg
    32941 taaaaagaaa cacagtcagt cattatttga accagttaaa tgatgagcaa ctactagtca
    33001 aaatcaattc tcgaccagtg atttatttcc acaaagaagc ttttgaaaaa caatttttta
    33061 agttacgcac gaatttttat ttttctatcg atgatctcaa agaggaacag ccttttttcg
    33121 cgcaaccaaa agacttattc tcttttatga tcggatacga cgccagtttg aaagaaagta
    33181 tcgaacaaat caaaacagct ctttactatc cagatggcgg attaccactt ttgattacag
    33241 gcgaaagtgg cacaggtaaa agttatttag ttaatttggt ctatcagtat tgtctgcttc
    33301 atgatctgct agaagattct gcaccattca tcacttttaa ttgtgcacaa tatgcagata
    33361 atcctgaact tttgactagc aatttgtttg gacatgttaa gggagcgtac actggtgcag
    33421 aagaaaataa aaaaggggct tttgaagcag cagatggtgg gatcttgttt ttagacgagg
    33481 tacatcgctt aacaccagaa ggtcaggaaa aactgttcac ctatcttgat caaggtgtga
    33541 tttatcgtat gggagaacct aatttgtcac gccggatcaa aacacgtctc ttttttgcta
    33601 cgactgaaga gattactagt catttcttaa caactttcat tcgaagaata cctatccgaa
    33661 tcgaactacc tgcacttaat cagcgtagtc gtaatgaacg gctagaactg atttactctt
    33721 tttttctcga agaacaaaga aaaatgggga gccctttgtc agtcagtgga ccagctttgt
    33781 cacttttaac aagtcagaaa ttatccggaa acataggaga gctgaaaaat atcgtcaaag
    33841 tttcagcagc aaaagcatat gctgaacagc gtgaacagcc tgaaatccat gtcacgatcc
    33901 atcatttgca aaaagaacta cttgcgcagc ctattactaa aaatacagca cagcaaggaa
    33961 tctatattac gcaagaccag accttagagc aattaatgga gaagcaacag cccgaacagc
    34021 agcgaatcgt taaaagtttt gagcaaatcc taattgattt caagaaaaat aactgtctgc
    34081 tatattcttg cgaaggaaaa ttaaagcaag aagtgattca attatttgat tttttgctgt
    34141 ttgagaccag tagacaagaa aaacacgagc tgctggttta ttggacacaa tctatccgag
    34201 agacctttcg acaaatggaa actgcctatc aaatcaaatt cgatggaaac agtgtctatg
    34261 ccttgagtta ctatctctat cagcgaagca ctatcaaatg gttaccggaa gaacatgata
    34321 ttatccaact aatcaaagaa ttagaacaac aagtttctca gtcttatcca tctagttatc
    34381 actatgtcca acgaattctg gaattaagta agatcaaatt agatattgaa gacacaagta
    34441 tggatcggat tttactgacg atctatctaa aaaaagcgaa atggtcaaaa gaaaaacgac
    34501 tgccaaaagc gatgattgct gctcacggtt atgcaactgc tagcagtatg gcaaatgtag
    34561 taaatcgttt attacaagag gatctatttg aatcatttga tatgccacta gatgtcacac
    34621 cacagcagat tgcttcagaa atccttgatt attgcgaaaa cagcgatgtt tcaaatggat
    34681 tgatcatatt agtagacatg ggatctctga aagaaatcca tcagtttttt aaaaaacagt
    34741 tatccgtacc gcttttgatt ttgaataatg tgacgacacc tttagcaatc acagtagggg
    34801 aatgcctgca aaaaaatgta tcattagaag agatagcaga agagactgtt cgacagatcc
    34861 agccagaatg gcgactgctc tatccagagg aaaacaaacc caaagcactc attactactt
    34921 gcttcactgg aatcggtacg gcggtccact taagtgaact gctagaaaaa agcttaccta
    34981 cggcttgtca gttgaaaatc atcccatatg aatatcagca attaaaagat aagaaaaaca
    35041 gtgacccatt attttccata tacgaagtcg taggaatgat tggcaccaca gatcctgata
    35101 tcaccaatat cgactatctt tcattagaag acctcatctc gggggaaaag atgtctgtac
    35161 tagctgaatg gttggacact actatgaatg cttcagagaa ggaaatattc aatcagcgca
    35221 tcattcgtaa tttttcattg gaaaaagtac tggattccgt gactattcta gatacagaga
    35281 aggtgatggg agaaatcgat cattttatga gagaattaga ggtgcagttg aatcgaagca
    35341 tcagcaatcc caaaaaatta gcgttgtatg tccatgtgag ctgtctcatt gaacgattga
    35401 tccgacaaat gccaatcgaa acctatcaag gattagagaa atggaagcag tgtcaaaaag
    35461 aagggttatc tgctattaaa tctgcgttta gtgtcattga agagcattat agtgtcatga
    35521 tccccaattc tgagattgcc tatatctatg atattttgag tgaagataca gagtttctct
    35581 cgatagaaga agaattttga acataagcaa gttgttgcca ctaattttgg catgcaactt
    35641 gcttttttag tatgtgtaag tgaaaagagg agatgaaaaa gtggatagag aaattatctt
    35701 agcttcccat ggaaaatttg cttcagggat attagattca ttagagctga tctatgggaa
    35761 aaatcattct attactgtat tcgattgcta tactgatgag aattttgatt tagcggaaac
    35821 agtaaggcag ctttttatga ggtacaaaga taaagaaatc attgttctga cggatctttt
    35881 tggcggaagt gtcaacaacg aattcttaca atatatcaat cgggatcatg tctatctcgt
    35941 tgccggattg aatttgccac tattgattga gctagtatct cggttggacg ctgaagtaga
    36001 gacaactgtt ttgatccagc aggtgctaac tgattcaaaa aaaatgatcc agttttgtaa
    36061 cgagcggctc aatcagaaaa tagaagaaga cgaattttag gaggaagaaa aatgattgta
    36121 ttgacaagag tcgaccatcg gttattacac ggacaagtag ctttttcatg gacacaaaca
    36181 ataggcgcag actgtatttt gattgctaat gatgatgtac ctacgaatga gatacgaaag
    36241 acaacgatca aattagccaa gccacaagga gtcaaactag tgatcaaatc aattgatgac
    36301 tcgattgccg cgttgaaaag cggggtgaca gataaatata agttattcat cgtagtggaa
    36361 tcgatcgagg atgcatataa gctggcagaa gcgtatccag aaatcaaaac catcaactta
    36421 ggagggatca aggcaaaacc tggtactcgc agcatcggaa aagcggtaaa tattttacca
    36481 gaagaggagg aacgcttgaa acgattaatt gacaaacaaa tagaagtaga aatccgtcaa
    36541 gtaccagcag ataaaaaagt ggatgtaaga gaagttttgt aagaaaggaa gaacctcaat
    36601 gattgtacaa gcagtcttat taggaattgt tgcatttgtc gcacaatcag aatatgcact
    36661 agggacatcg ttgctttctc gtccgattgt gacggggctg tttacaggaa tcgtgttagg
    36721 tgatgtaaaa actgggatca ttatgggcgc aactttagag ttagcattta tcggatcgtt
    36781 ctcagtaggt gcttcgattc cacctgatgt tgtgaccggt gggattttag ggacggcttt
    36841 tgctattaca gctggagccg gtacagaaac cgcgctttta ctaggtttgc cgattgctac
    36901 gttgacattg atattgaaaa atatttactt agggcttttg attccaatta tgaaccataa
    36961 agcagacaat tatgcagaag aaggaaatta taaaggagtc gagcatatgc acttgcttgc
    37021 cggtatcggt ctttccttga tgcttggtgt tgtggtaatg gtttcttata tggtgggaag
    37081 caatacgatc ggaaatctat taaatatgat tcctgacttc gttcaaaagg ggttgtcagt
    37141 agctacaggg ttgattccag ctcttggttt tgcgatgctt gctcgtttgc tgatcaacaa
    37201 acaagttgca ccatattttt tcttaggttt tgctatagct tcttacttag agattcctgt
    37261 gaccggagta gctatttttg gtgcgatttt agcagttgtg gtagtcaata ttatgaatgt
    37321 ccgtcaacta ccacttcaaa caactgcagg ggaggtagaa gaagatgaag aattctgaga
    37381 tcaaaaacag caaaataacg aaaaaagagc taaatcacgt attttggcgt tcttttcaaa
    37441 tggagttttc ttggaattat gaacgtcaga tgaatatggc ttatgtttat gcaatgattc
    37501 cgattttgaa aaagttatat cagtcgaaag aagaaatggc agcagctttg aaacggcatc
    37561 tagagttttt taacacgacc ccacatatcg ttacactgat tttaggaatc acggctgcaa
    37621 tggaggaaga aaataaggat gatcctgaat ttgatgtgac tgcgatcgat agtatcaaaa
    37681 catctttgat gggacctctt gcagggttag gagattcttt cttttgggga actttgcgat
    37741 tgattgccac aggtgtgggt acctctttag ccatgcaagg aaatatttta ggccctatct
    37801 tgttcatttt gattttcaat attccgcaca ttctgtttcg ctatctagca acaggttggg
    37861 gatatcgctt aggaacgggt tttttgaaaa agatccaggc aaacggtatg atggaaagtt
    37921 tgacattagg cgcgtctatt attggactga tggtcgttgg agcaatgact gccacgatga
    37981 tcaccatcaa tattccttta aaaatcggtt caggagaaaa tgctgtgact gttcaaagta
    38041 tttttgatga tatcgttcca aatatcttat gtcttggagc atttggcata gtgttttatc
    38101 tattgaagaa agaagtgaaa ccattaacga tcttgatcgg actagctttc tttggaattt
    38161 ttggttcgtg gatagggatt ttttaaaaaa ggagcagaga aaatgtttac gatgcaagat
    38221 tatatctatg aagaaaagga agtactaagt acgatcttga aaaagaatga tttttcgaca
    38281 agagaacata tgaaaaaaac agtgaattta ctgatcttag caacgggttc atcttataac
    38341 gcttgtttag cagcgaagcc agcattggaa tcttatggtg atcttactgt cgatattcaa
    38401 gaaccttttt attttttcaa ttatggaaaa ctttctcctt caatcgatac tgtgattgct
    38461 gtgtcacaaa gcgggaaaag tgcttctact gttgaagcag taaaaatgat acaaaaacaa
    38521 gggttacctg ttgttgctat cacaaatgac gtgcaaagtc cacttgcttt agaagctgca
    38581 caaattattg atttaggtgc cggggtagaa tcagtaggct tcgttacaaa aggctattct
    38641 gcgactgttt tacagttact actcttaggg ataggaattg gaataagtaa aaataaaatc
    38701 agcaaaaaaa tagaacaaga ctacatgcaa cagttgcgca aaataatcaa ccatttacca
    38761 gcgattattc aaaaaacgga agaatttttt gatggatatc aaagtttatt tcgtttagca
    38821 cagcgtttcg tcaccattgg atatggtcca aactggggaa cagctaaaga agcagagacg
    38881 aaattgactg aaacaatccg cgttccgtca caaggatttg agttagaagc ttatatgcac
    38941 ggcccatacc tggaggcaga tgcttcgcat ttgctattct ttattgaagg ggaaagtgta
    39001 aataaggaac gttcgcaaaa gttgcaaaga tatatgagtc ggtacgttgg agagactctc
    39061 accattacca caaaaaaagc aagaaacgag aaaacacttg gattagcaat agaatgtgat
    39121 gaatatctgt ctgtattagc gcttgtcgtt ccgtttcaac tattcgctta taaaaccgca
    39181 gtagctaaag gtattgactt gaacaaaaag atttttgaag actttgatac agtcttgaaa
    39241 agtaaattat aagaggtgga aatcatgctg aaattcaatg aagaagaaca aatcaaagca
    39301 ataaaaggcg cactagcttt acgtccgcaa gtagaagaaa tcatcgataa actttatgcg
    39361 gaaaaatttg acgctgtcta ttatctagga atcggtggaa cgtatgcttc ctcgatgcaa
    39421 gctgtcactt atatgaatgg gaaaagcaac ttgcctgtct ttgtacagca tgctgcagaa
    39481 tattacacta ctggaaacaa aagattaaca aaagattcta tcgttatttt gtcctctgtt
    39541 accggtacaa ctcaagaagt agtgaaagca gtagaacaaa tcaagaaagt aggagcgaca
    39601 ctgattggat ttatcgataa agcgaacagt aagttgtctc agctatgcga ttttgtggtc
    39661 acttatccag ctcctggaac agaacaaatc aaatttttta tggcagcaga tcgattgatg
    39721 tatttgaatg gagaattcga agcttattca gaatactatg aacagctcga acgttattta
    39781 ccaacagggt tagtggaagc agagaaaaaa gcagatgcat ttggcttatc atttgcagaa
    39841 aaacatcgac gtgatagtat gcattatttt attggagcag ggaatcaatg gggagctgtt
    39901 tattcatatg ccatgtgtta ctgggaagaa caaagctggc ttccatcgaa atctatccat
    39961 gctgccgagt ttcttcacgg gacgttagag atcgtagaag aaacaacccc agtcacatta
    40021 tttttaggcg aagatgaaca gagagtgtta tctgaacgtg tggctaaact tttgccaaaa
    40081 atttgcagca attatacatt gatagacaca aaagactatc cagtagaagg tatcagtgaa
    40141 aaatatcgcg gacgtgtttt gtctttctta ttgatgcatg tagtgacaca acgcatcgat
    40201 gctcatgtgg agcagttgaa ctgccatcca ttagagatac gccgctacta tcgtcagttt
    40261 gattattagg atagactgga taaaaagaat ataaaaaata aagagagcag cgataactgc
    40321 tccctttatt tttttaaaat attggcatca attacgttat aaaacacatt ttttgcatta
    40381 tctacagttc aacttataat ctgtttaatg ggacatgtgg gtgccaacca ttttttgtta
    40441 tgcaatagta ctatttagtt gttgcatgga tccgtagtaa aagtccaagt acaacgagtt
    40501 gcgccagttt acatagcgtt ttgtatccag aatgttgact aaaacaaaag ttcctatgtc
    40561 aaccgtcaac agagacaatc tgtccgttgc aggacctgac agatttagaa atgttcaatc
    40621 tgtctcttat gtattttaaa tcaatactca ctaggagaag tttcctaacc tcctaatagt
    40681 aatgctggta gtaacattgt caaaactgct tcctcttagg aacaagcaaa gcataacgag
    40741 tgtaaaaata aaatgtagac tattgtaaca tatcattgat tatttcaagt aaaaacgata
    40801 aaagaagtaa acaaacactt aaaaacaatg gaaattagtc ccaactttaa gaaatgtata
    40861 ctataatgga aattatcaat tatttatttg gaggaatatc catgaaacaa aaaatcgcag
    40921 ttcttggtcc tggatcttgg ggcactgctt tagcacaaac attggcagaa aacgggcatg
    40981 atgtccgtat ttggggaaat gtacctgaac agattgatga aatcaataag tatcatacaa
    41041 atcagcattt tttacctgat ttgacgatcc cagaatcgat tatcggatat aaagaattgg
    41101 cagaagcagt ggataacgca gatgctattt tatttgttgt tccaacaaaa gcgattcgtt
    41161 cagtcgcaca ggaacttatt ggtaaaatga atactaagcc aatcattata catgcaagta
    41221 aaggactaga acaagatacg cataaacgaa tctcagaagt tttggctgaa gaaattccag
    41281 aagaaaaaag acaggcaatt gttgtattat ctggtcctag ccatgctgag gaagtggcag
    41341 tacatgatat cacaacaatt acagctgcaa gtattgatca aaaagcagca gcctatgtac
    41401 aaaaactgtt catgaatgat tatttcagga tttatacaaa taatgatgtg attggcgtag
    41461 aaactggtgc agcactgaaa aatatcattg caattggtgc cggcgctatc catggattag
    41521 gattcggaga tgatgcaaaa gcagcaatca tgactcgagg attggcagaa atcagccgtc
    41581 ttggtgtcgc gatgggtgcc aatcctctga catttatcgg tttgagtggt gtgggggact
    41641 tgatcgttac ttgtacaagt gttcattctc gcaattggcg tgccggtaac ttgttaggac
    41701 agggacataa gctggaagaa gttctagaaa acatgggaat ggtcgttgag ggtgtggcaa
    41761 cgacaaaagc agcagtagag ctagctcaac agctaggtgt tgagatgcca attactcaaa
    41821 caatctataa tgttttgtat aatggagaag atatcaaaaa agcagcaaaa gaaatcatgt
    41881 tacgtgacgg aaaaatggag aatgaatttt ctcttcgtta aatagatgat caatagttaa
    41941 tcgt
//
LOCUS       DACDXE010000018        41458 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.018, whole genome shotgun sequence.
ACCESSION   DACDXE010000018 DACDXE010000000
VERSION     DACDXE010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.018"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1237..1902)
                     /locus_tag="IWM23_000972"
     CDS             complement(1237..1902)
                     /locus_tag="IWM23_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883633.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2028..4064
                     /locus_tag="IWM23_000973"
     CDS             2028..4064
                     /locus_tag="IWM23_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAP9883634.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4068..4706
                     /locus_tag="IWM23_000974"
     CDS             4068..4706
                     /locus_tag="IWM23_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883635.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            4734..5162
                     /locus_tag="IWM23_000975"
     CDS             4734..5162
                     /locus_tag="IWM23_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883636.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            5619..6530
                     /locus_tag="IWM23_000976"
     CDS             5619..6530
                     /locus_tag="IWM23_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9883637.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            6747..7184
                     /locus_tag="IWM23_000977"
     CDS             6747..7184
                     /locus_tag="IWM23_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883638.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            7279..8163
                     /locus_tag="IWM23_000978"
     CDS             7279..8163
                     /locus_tag="IWM23_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAP9883639.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            8387..11554
                     /locus_tag="IWM23_000979"
     CDS             8387..11554
                     /locus_tag="IWM23_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883640.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            11574..12119
                     /locus_tag="IWM23_000980"
     CDS             11574..12119
                     /locus_tag="IWM23_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HAP9883641.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKIQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            12147..12602
                     /locus_tag="IWM23_000981"
     CDS             12147..12602
                     /locus_tag="IWM23_000981"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HAP9883642.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            12599..13357
                     /locus_tag="IWM23_000982"
     CDS             12599..13357
                     /locus_tag="IWM23_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9883643.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            13858..15057
                     /locus_tag="IWM23_000983"
     CDS             13858..15057
                     /locus_tag="IWM23_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883644.1"
                     /translation="MLFTSGPDRAFERLMQEKPGFDHYDGGGADAPEDCGTCRFYRPD
                     WKYQFCQFAECPYQPGKLTDYAAVKFTVKGANNAMAVFRVEKNKGYTVMSNHHLRNKN
                     LTLKAKGLLSQMLSLPEDWDYTLKGLSYINRESIDAIRTAVWELEKAGYIRREQGRDA
                     KGKMADMVYTIYEQPVLENPVLENPTSDNPVLENPTSDNPASENPTQLNKEVQRTDLS
                     KKEKIITDGQSTHSIPIPSPTPAPMEGEAAATPPERKRTGKDEAVRIYREIILENIEY
                     AYLIQDRSIDREQLDEIVDLMLETVCTARKTIRVAGDDYPAELVKSKYMKLTGEHIRF
                     VLDCLRENTTKVRNIKQYLRAMLFNAPSTISNYYTALVAHDMAQPDWGKPKSGLPDYS
                     CGEGESL"
     gene            15079..15291
                     /locus_tag="IWM23_000984"
     CDS             15079..15291
                     /locus_tag="IWM23_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9883645.1"
                     /translation="MKQGALIFDERTDRYDIRFDLADYYGGLHCGECFDVMIGGKWRP
                     TRIEMAENWYLVGIRADDLSGLRVRI"
     gene            15366..15842
                     /locus_tag="IWM23_000985"
     CDS             15366..15842
                     /locus_tag="IWM23_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HAP9883646.1"
                     /translation="MQEEVTQKTIALSMKTGKLTAQVLQAALKKYLQHRAKGKNTLHH
                     GQQSLKQLKKHGADLSNIEITEANIGAFKPCAKKYGVDFTLRKDKTTQPPHYVVIFKA
                     KDADNLDLAFREFTAKTLSKEQRPSIRKVLSAVKQKAAAQTKQRAKEKIKERGLEL"
     gene            15839..17647
                     /locus_tag="IWM23_000986"
     CDS             15839..17647
                     /locus_tag="IWM23_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAP9883647.1"
                     /translation="MKPDVKKLLILNLPYLIFVYLFAKCGEAYRLAAGADLSGKLLHF
                     ADGFAAAFANPLPSLHLFDLCIGIAGAVIVRLVVYCKSKNVKKYRKGMEYGSARWGTA
                     KDIAPYIDPKFENNILLTQTERLTMTGRPKDPKTARNKNVLVIGGSGSGKTRFFVKPN
                     LMQCFPTTDYPTSFVVTDPKGTLVLETGRMFQQAGYRIKILNTINFSKSMKYNPFVYI
                     HSEKDVLKLVNTLISNTKGEGEKSAEDFWVKSERLFYSALIGYIWYEAPAEEMNFSTL
                     LEMINASEAREDDPEFQSPVDLMFERLEQKDPDHFAVRQYKKFLLSAGKTRSSILISC
                     GARLAPFDIREVRELMEDDEMELDTIGDEKTVLYLIMSDTDTTFNFILAMLQSQLINL
                     LCDRADDVYGGRLPVHVRLILDEFANIGQIPNFDKLIATIRSREISASIILQSQSQLK
                     AIYKDAAEIISDNCDSVLFLSGRGKNAKEISDALGKETIDSFNTSENRGSQTSHGLNY
                     QKLGKELMSQDEIAVMDGGKCILQLRGVRPFLSEKFDITKHPRYKYLADADKKNTFDV
                     DRFLTITRRKRQQVVTQDEVFDLYEIDLSDEDAAAE"
     gene            17724..17939
                     /locus_tag="IWM23_000987"
     CDS             17724..17939
                     /locus_tag="IWM23_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer protein"
                     /protein_id="HAP9883648.1"
                     /translation="MAFFNSAVTTLQTIVIGLGGALCVWGGINLLEGYGQDNPGAKSQ
                     GVKQLVAGGGVALIGVTLVPLLSGLLG"
     gene            18031..18894
                     /locus_tag="IWM23_000988"
     CDS             18031..18894
                     /locus_tag="IWM23_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002574003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883649.1"
                     /translation="MISEIIEEWIKGILMDGITGNLSGLFDTVNAKVGEIAADVGRTP
                     QAWNSGIFNMLHSLSETVIVPIAGAILALVMCYELIQMITEKNNMHDFDSSMFFRWIF
                     KSAFAILIVTNTWNIVMGVFDATQSVVNQSAGVIIGQTSIDFNTLIPDLESQLEAMSV
                     GALLGLWFQTLVVGLTMNILSICIFLVTYGRMVEIYVVTSLGPIPLATMGNAEWRGMG
                     QGYLKSLLALGFQAFLIMVVVGIYAVLIQNIGSASDISGAIWGCMGYTVLLCFCLFKT
                     GSISKAVFGAH"
     gene            18925..19467
                     /locus_tag="IWM23_000989"
     CDS             18925..19467
                     /locus_tag="IWM23_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HAP9883650.1"
                     /translation="MFISTYNIIYADPPWRYDCKNVQGAAENHYSTMSIDELCALPVE
                     RLASKDCLLFLWATFPMLPEALRLIKAWGFSFKTVAFVWLKQNRKSLTWFYGLGRWTR
                     GNAEICLLATHGKPKRRSASVHQFIISPIEQHSKKPDVTREKIVELAGDLPRVELFAR
                     QKTPGWDVWGNEVECDLILT"
     gene            19481..19903
                     /locus_tag="IWM23_000990"
     CDS             19481..19903
                     /locus_tag="IWM23_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9883651.1"
                     /translation="MAYVTVPKDLTHVKSKVVFGLTKRQLICFSGALVIGVPLYFLTR
                     DYLTNSAAALLMVFAMLPGFLLAMYERHGQPLEVVIMQIIQCCFIQPKERPYQTDNTY
                     TALVRQYQMEQEVKAIVQKGNPKRNRKNKADPRPAKRN"
     gene            19833..22232
                     /locus_tag="IWM23_000991"
     CDS             19833..22232
                     /locus_tag="IWM23_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HAP9883652.1"
                     /translation="MSKKEIPRETEKTKLTRAQRKEIDAVIRKYKGDGRPHTAQQSIP
                     YEVMYPDGVCRVSPGVFSKCIEFADISYQLAQPDTQTAIFEKLCDLYNYVDASIHIQF
                     SFLNRKVDPVQYAKSFEIAPQGDDFDDIRAEYTGILQKQLANGNNGMVKTKYLTFTIE
                     AESVKAARARLKRIGFDLLGYFKSMGAVAHVMDGWERLNLLHGVYHPDGEIFNFDWKW
                     LAPSGLSTKDFIAPSSLCFGNAKTFGMGGKYGAVSFLQILSPELSDDMLADFLNTESG
                     VLVNLHVQAIEQTKAIKTIKRKITDLDAMKIAEQKKAVRSGYDMDILPSDLATYGEDA
                     KKLLTKLQTRNERLFQLTFLVLNVADTKQKLNNDVFQAAGVAQKHNCPLVRLDYQQEQ
                     GLASSLPLGVNQIKIQRSLTTSSVAVFVPFVTQELFQGGAAMYYGINAKSRNMIMLDR
                     KQARCPNALKLGTPGSGKSMSCKSEIVSVFLTTPDDIFISDPEAEYYPLVKRLHGQVI
                     RLSPTSKDFVNPLDINLNYSEDDNPLALKSDFVLSFCELVMGGKNGLEAIEKTVIDRA
                     VRVIYRPYLADPRPENMPILSDLHKALLDQHVPEADRVAQALDLYVSGSLNVFNHRTN
                     VDIGNRLVSFDIKELGKQLKKLGMLIVQDQIWGRVTANRSQGKATWYFADEFHLLLKE
                     EQTAAYSAEIWKRFRKWGGIPTGATQNVKDLLSSPEIENILENSDFITLLNQASGDRK
                     ILAERLNLSTEQQKYIDNSEPGEGLLIFENVVLPFTNPIPHNTQLYKIMTTRLNEVAG
                     V"
     gene            22264..24255
                     /locus_tag="IWM23_000992"
     CDS             22264..24255
                     /locus_tag="IWM23_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006060479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9883653.1"
                     /translation="MKELKAKAKVTQAMTRDGLTLENQATGEVENISSREAEQDLSAP
                     GPGGTADKLLERADAIHDGHKAKKAAKQAGAVAAEGDAALHRPSARLQLSEEERADPD
                     LQPYIRKSDKRADKLDKARAAIPKKRVPVKETVFDEASGKAKTVLHFDKQDKGPPSLK
                     PNPAGRPVSEVFLFGHGKIHEVEHENVGVEGGHKGEELAERQAGKAIRSTIRHHRLKP
                     YWDAEKAERRLLSANAEYFYQKSLRDNPEIAQAASNPISRFWQKQQIKRQYAKAARTA
                     GQTAGAAAQGAAKTAGASTKAAKAAAKESERAASFVARHWKGALAVLVLGLLMLLIIG
                     GLQSCTAMFGSAGSGITASSYFSEDSDMLAAEDAYAELEAELQDYLDNYEATHDYDEY
                     HFDLDEISHDPYVLISILSALHDGIFTIGEVQGDLSMLFDKQYILTENVVVETRYRTE
                     TDTWTDAEGNTHTDTYQVPYDYYICYVTLENFDLSHVPVYIMDEETLSFYSLYMSTLG
                     NRPDLFSGSQYPNASTLTPPTYYDVPPEALEDATFAAMLKEAEKYIGYPYVWGGSSPS
                     TSFDCSGFISWVINHSGWNVGRLGAQGLYNICTPVSSSQAKPGDLVFFKGTYDTPGVS
                     HVGLYVGNNVMLHCGNPISYTNLTSNYWQSHFYSYGRLP"
     gene            24278..24529
                     /locus_tag="IWM23_000993"
     CDS             24278..24529
                     /locus_tag="IWM23_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4315 family protein"
                     /protein_id="HAP9883654.1"
                     /translation="MATKLDRIEKDIQKTKSKIAEFQKQLRELETQKTEQENLQIIQL
                     VRGMNMKPEEFAAFLRSGAMQAAPAATPYHEQEDNAHEE"
     gene            24519..25748
                     /locus_tag="IWM23_000994"
     CDS             24519..25748
                     /locus_tag="IWM23_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4366 domain-containing protein"
                     /protein_id="HAP9883655.1"
                     /translation="MKNKTRRISRILPALLAVMLCVTAFPVTALAGGNDPAPAPLPEA
                     TTEPATGGLEPETDETGTATDGTETEAEKDTGPLTGKDITDLFSALFGSKVSIAATDD
                     GIQITTGTDKKEPEQTGTVTTNGGRLNVRTGAGLDKTAFTQLPNGTTVEVVGTDGDWI
                     KILLPERIGYVHSDYMTVSEKEVAATGEGGFSLSIDPEEIASLLELFNGGGASAALSP
                     DGNLSLIDDIGSAAKNGKQFITVETKNGNVFYLIIDRDDEGKETVHFLNQVDEADLLT
                     LMEDGETVTPAAVCSCTTKCKAGAVNTNCPVCKTNLTECSGPEPQEPQPEEPEAPEEE
                     PKGGAGGLIVFLLVALAGGGAALYYFKFKKPKAETTGSDDLGEYDFGEDEDLEDEEPE
                     TEFDPEADFVSEQEDEE"
     gene            25745..27826
                     /gene="topB"
                     /locus_tag="IWM23_000995"
     CDS             25745..27826
                     /gene="topB"
                     /locus_tag="IWM23_000995"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9883656.1"
                     /translation="MTYKLVIAEKPSVARSIAGVIGATEKHDGYLEGNGYLVSWCIGH
                     LVSFADAGRYDERFKKWRYEDLPILPETWQYIVPDDKKQQFDVLCSLMQRPDVTGLVC
                     ATDAGREGELIFRFVYQMAGCQKPFERLWISSMEDSAIKDGFAHLKPGGDYDPLYQSA
                     LCRAKADWLIGINATRLFSVLYHKNLTVGRVQTPTLKMLVDRDAKITDFKKEKYHIVH
                     ITAGGADAVSSRFSDAAEANTVKAACAKAQAVCVSVTREKKTEQPPKLYDLTTLQREA
                     NRLFGYTAKQTLDYAQALYLKKLLTYPRTDSQYLTDDMLPAVESLVSGLWELVPFAKG
                     LNLSPQFDRILNSKKVSDHHAIIPTAEFVKQGFGTLAESEKKLLSLICCKLLCAVAGP
                     HIYEAVTATFTCAGQEFTAKGKTVLSPGWKEIERRFRSSLKTDADEDAEAVRELPELC
                     EGQTFADVAASVTEHFTTPPKPYTEDTLLSAMERAGAEDMPEDAERKGLGTPATRAAI
                     LEKLVQMGFVQRKGKQLIPTKDGINLAVVLPEALTSPQLTAEWENRLTEIAKGQADPD
                     EFMDGIETQARELVQTYSCISEEKQKLFQAERVPIGTCPRCGEAVYEGKKNYYCGNRA
                     CQFVMWKNDRFFEERKKAFTPKIAAALLKTGKVKVKGLYSVKTGKTYDGTVLLADTGG
                     KYVNYRIEHRS"
     gene            27975..31895
                     /locus_tag="IWM23_000996"
     CDS             27975..31895
                     /locus_tag="IWM23_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4316 domain-containing protein"
                     /protein_id="HAP9883657.1"
                     /translation="MNEVFSIQVHNRQFLEQGKQGVWLDLPTTTETLQAALRDVGITA
                     DNPQDFFINGYSCPEDRHLAIPYDMVLAADVDELNFLAARLGQLDAAEIAELNAALQN
                     PRGGFENIGQIIDYTENVDYYVHLPDVRGDGQLGDYYLNRSGMVDMPEEWKAGIDTAQ
                     FGRHIAQQEQGVFTQYGYLVRSGDEWQRVHEGQPVPEEYRVMHYPQPEADREEPQAQQ
                     PEASESPDKAPPPVIPIMLDGMNSKERMKVITDRLETGVQELFESSRYMAYLSAMSKM
                     HDYSLNNTLLIAMQGGSIVAGYNRWQDEFDRHVKRGEKGIKILAPAPFKVKQQMEKID
                     PATQQPVIGADGKPVTEEQEVTVPAFKVVTVFDISQTEGKDFPMPYISELTGDVQQYQ
                     DFFAALERTSPYPISIEPISDGAKGRCFYAKQRIAINEGMSELQTLKTAIHEIAHAKL
                     HAIDREAPAEEQAARPDRSTREVQAESVAYTVCQYYGLDTSDYSFGYVADWSSGRELS
                     ELKASLETIRATASELITEIDGHFAQLQQEREARQEQEAVTVDVTPEAAAPAQAEEEQ
                     APALDPAAEPLVTVLWSESPQLKDGQQMPLHEADALFARLDAAYPAEAGYDKTAFRID
                     CIFQGEPDSYEGRQDFGDRDGSLIEHIQSYHQFYAQDENWKKHVIATTGIEGWEQDKA
                     HRDMLLGEFVPYMKLHCSLAQMEQTAQGRLQSGETLTPEETAYLGALLSYVQECRPML
                     NQGQYQLPEPPKLADFDQSLQDYKAQVQAEIAQEAAAAGMTVEEYAAAGYEAPQQDTF
                     SIYQLRGGEETRDYRFEPYERLQAAGLAVDSANYDLVYTAPLDKSTSLEDIYRTFNID
                     HPADFRGHSLSVSDVVVLHQNGQDTAHYVDSFGYMEVPEFLRENPLRTAELSTEQNEN
                     MIDGIINNTPPEPQPEEPEQLSFEDVAVASGQTQEEPPEAAPPQTAVRYYPINEGAAR
                     RAKEAMSFSDYRPGSATAEYRHYVDQAVEIAERQKKRVEPEYHEKIDSLLDTYARKLA
                     ENMNKGYEITARVPSVLIAGPSNFPVRAKEKQNAASDRNMEEFQYIQGLLDKIRSTGM
                     GGISADDPQAVSKLEKKLEKLEASQELMKAVNAYYRKHGTLDGCPHLTERGIENLKAD
                     MASGWHYEKKPFQSWQLSNNNAEIRRLKGRIEELTRQKEAAYVGWEFDGGTVEINRVA
                     NRLQIFFEGKPDAAVRDELKSNGFRWSPKAGAWQRQLNDTTIRVADRIKCIQPLSGEK
                     PSALQLAARREKEKPSIRAQLNAAKTEQAKQPPAREKSKGLEVE"
     gene            31896..32840
                     /locus_tag="IWM23_000997"
     CDS             31896..32840
                     /locus_tag="IWM23_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002417119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883658.1"
                     /translation="MQKFENVDVLAALEQIMRQNTAFYQSDFGIDKDIIRRDAASDQA
                     ADKTLLWMSRPSGTYCFRERDVFLKDTRQYNTWKFYGEQTRDKILAYAVELTGTQGGT
                     IRGNLYELDYPQHFRHVIETAQPVSVNRLFYEHGTRDIPESQRFDGSPDRVLGNFLYY
                     EAQPHDPAVLQEALRTEQHSRGRAVPGDFKVHVTALHDSRIETEARRIVDSLKDLSAP
                     NSPNKTHFMVEISPHFAAIASTKDNDRLFAMLPYKSLCFTGMKGRHGVYAVINRDERR
                     DVSIRRPRPSIRKQLSDSKQAASPKKAAARTKKNELEV"
     gene            32844..33047
                     /locus_tag="IWM23_000998"
     CDS             32844..33047
                     /locus_tag="IWM23_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883659.1"
                     /translation="MMHFTVEEENLICMYHNADRRRTINRLTAVMPVMDGDMRAQAQQ
                     TLSKLERMTDADYDGQKFHFTDE"
     gene            complement(33347..33583)
                     /locus_tag="IWM23_000999"
     CDS             complement(33347..33583)
                     /locus_tag="IWM23_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883660.1"
                     /translation="MDGQEKLLDYETIKAAVAGERWATEKVLAHYADYINELSTVEIR
                     QANGKKKKVIDEDIRQRISLKLLEALPSFPLEQR"
     gene            complement(33766..34137)
                     /locus_tag="IWM23_001000"
     CDS             complement(33766..34137)
                     /locus_tag="IWM23_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883661.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITKDRDEVKRCCECGATFTPKSNRAKYCAACSKRM
                     RRRKEAERQRKRYWKPTHLEL"
     gene            complement(34134..35462)
                     /locus_tag="IWM23_001001"
     CDS             complement(34134..35462)
                     /locus_tag="IWM23_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9883662.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(35423..35752)
                     /locus_tag="IWM23_001002"
     CDS             complement(35423..35752)
                     /locus_tag="IWM23_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9883663.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(36010..36381)
                     /locus_tag="IWM23_001003"
     CDS             complement(36010..36381)
                     /locus_tag="IWM23_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9883664.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            36526..36711
                     /locus_tag="IWM23_001004"
     CDS             36526..36711
                     /locus_tag="IWM23_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9883665.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            36890..37036
                     /locus_tag="IWM23_001005"
     CDS             36890..37036
                     /locus_tag="IWM23_001005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883666.1"
                     /translation="MTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVSRHWITAG
                     LMRL"
     gene            37654..38313
                     /gene="vanR"
                     /locus_tag="IWM23_001006"
     CDS             37654..38313
                     /gene="vanR"
                     /locus_tag="IWM23_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9883667.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            38316..39659
                     /gene="vanS"
                     /locus_tag="IWM23_001007"
     CDS             38316..39659
                     /gene="vanS"
                     /locus_tag="IWM23_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9883668.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
     gene            39830..40636
                     /gene="vanY"
                     /locus_tag="IWM23_001008"
     CDS             39830..40636
                     /gene="vanY"
                     /locus_tag="IWM23_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9883669.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            40649..41458
                     /gene="vanW"
                     /locus_tag="IWM23_001009"
                     /pseudo
     CDS             40649..41458
                     /gene="vanW"
                     /locus_tag="IWM23_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT        11605 a         9535 c        10331 g         9987 t
ORIGIN      
        1 actacctgta ttagtcctct tttttgacca gattttgacc agtctatttt acattctaat
       61 atattcatat gagctaattt tatgattaaa tggtaagtgt ttccttgttt gttttagttt
      121 aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata cttaaataaa
      181 ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg aagcgaaaat
      241 aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat gcaaaaaaca
      301 gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt aagacaaaga
      361 gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac taggtaacta
      421 actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat ataactaaga
      481 aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc gtctttgttt
      541 taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga ggggcaaaat
      601 ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg attcctctat
      661 aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt tttacaatga
      721 aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat ttgtttcatt
      781 ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta ctcttggata
      841 aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata aaagtaaatg
      901 agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg cagcttgact
      961 acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc gaatgagcaa
     1021 aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt gggaggctgt
     1081 ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt ttatttagga
     1141 actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat aagatcatgt
     1201 gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg gctcctgagg
     1261 gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat ctgagggaat
     1321 ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt tgattacagc
     1381 atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac catggccaaa
     1441 gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag ggagtgtggt
     1501 atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat ttttgtattg
     1561 ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag atagtgtctt
     1621 tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag gttcatctgg
     1681 caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa tcggcagacc
     1741 taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag aattttccca
     1801 actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt gcgtaagctt
     1861 acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct cctttcgatt
     1921 ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt ttttttgatg
     1981 aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg ataatcaaaa
     2041 aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt tttgccttgg
     2101 ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa acagatactt
     2161 atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag cagctaatca
     2221 tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa acagtaaatg
     2281 aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt gaaatgacat
     2341 tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct aatacctcct
     2401 ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt gaatttttct
     2461 tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact gatcgattca
     2521 atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa aatagctatt
     2581 cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta actattggat
     2641 tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa gaaatcagtt
     2701 tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg gggaaaaaga
     2761 aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt atgtattata
     2821 aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga atatatggta
     2881 tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa gcgttcaact
     2941 atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg accgttgcca
     3001 atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc catgaaccac
     3061 caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc gggaaaaacc
     3121 acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa ttatatgaaa
     3181 ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat gaagaaaatg
     3241 aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt cctttgattg
     3301 atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt atattgatcc
     3361 ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca gaatattatc
     3421 aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa cccatttata
     3481 catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata ctgacattga
     3541 aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca ccgttaaaga
     3601 tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa agtcagttac
     3661 gtgataactt gcagttatca tttccgtata ctcagactga agagatagct gtcaaagtgt
     3721 taagcagatc atttcattac ttgatacaga tatttttact cactttcttt tctttcttct
     3781 tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa atcgctatct
     3841 ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg atgaacctca
     3901 tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag cctaaatatc
     3961 tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt tttattttgt
     4021 ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg gagccgttga
     4081 tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat ttggatcgtt
     4141 tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca ggtaaaagta
     4201 cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc tatttgttcg
     4261 atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga aataccatat
     4321 cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt aatttagata
     4381 ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag tatgaggcat
     4441 tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta tctggtggtg
     4501 agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc attttagccg
     4561 atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa atcttatcgc
     4621 aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat ctgaccaaaa
     4681 taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt tttatgaaaa
     4741 aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct atgtcatctg
     4801 aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag ccaggagaga
     4861 ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac cgggaaattt
     4921 ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat acaacttgta
     4981 cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac ggtgcaaaag
     5041 cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg gcctatgcaa
     5101 gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc ggttggtatt
     5161 aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg ttaaaagtta
     5221 tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat aataaaaaca
     5281 acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc ccagcagcgg
     5341 aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta aatacggaac
     5401 gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt gatgtgctac
     5461 ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc tattcaggac
     5521 gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac ctttgttttt
     5581 agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga tttttagcac
     5641 ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt gaaaaaacag
     5701 ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc ttgaaaaaaa
     5761 tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt gaagataaat
     5821 atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca aagaaacaat
     5881 tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa acattcaaag
     5941 acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc aaattgaccg
     6001 atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct caaattatcc
     6061 aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag ggagacgatt
     6121 tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat ggcggaaaag
     6181 tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca gcctttaagt
     6241 tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact tatactacgg
     6301 aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg gataaataca
     6361 aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc ttccagaata
     6421 aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa gactttgaaa
     6481 atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa gcggtataaa
     6541 taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt tattagaact
     6601 aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa aatttttttt
     6661 acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag aaaacaatat
     6721 caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt ttatttctag
     6781 aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga agtacaatgg
     6841 atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc acactaatta
     6901 tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta catcaagagt
     6961 ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac aaaaagtgga
     7021 agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct aagattttta
     7081 ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt gttttaattt
     7141 tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat gtttcatgaa
     7201 cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa agaaatcaat
     7261 cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag ttcaggcggt
     7321 gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt gtttgatgaa
     7381 gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg cgaagcggct
     7441 acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa cgaaggaatc
     7501 agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt gggtacggtg
     7561 aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat caatcatttt
     7621 tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta ttttatgaat
     7681 gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga gaagaagacg
     7741 aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc cacacaatcc
     7801 tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag taccttgctg
     7861 ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc aaaagtggat
     7921 gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac gcttaaaaca
     7981 ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta ccttacttgt
     8041 gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt tgatggagat
     8101 gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca ggtttattgt
     8161 taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg ggataaggga
     8221 ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt acacctaata
     8281 gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg aaatagatta
     8341 aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga atcaatgtgt
     8401 aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga catccgtttt
     8461 tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt ttgacaagac
     8521 tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg aggcttctac
     8581 taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg aacaaacgtc
     8641 tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca gcgaagctgc
     8701 cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga tccgactgat
     8761 tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta ttgattttgg
     8821 tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac ctcagtcgat
     8881 cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac caaatatcga
     8941 tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat atcattttac
     9001 gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat taggactgat
     9061 ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg atgtccaacc
     9121 agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa gctatcaatt
     9181 gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct acggttttct
     9241 agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata tcttaagcgg
     9301 ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag gagaggcagt
     9361 cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg aattttcggt
     9421 aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa tgagaggttc
     9481 aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag caactacggg
     9541 cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg gacaaatctc
     9601 ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt ttgtaggaaa
     9661 aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata agcagagtgg
     9721 gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa ccaatggagg
     9781 ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac tcaatcaagg
     9841 aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag gttcagctga
     9901 ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg gagcaactgt
     9961 agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt atttagtcat
    10021 ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt tgaatgtgga
    10081 cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg agaccaatgg
    10141 cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg cccattcaga
    10201 aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag acccggatta
    10261 tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt tcgtatcaag
    10321 ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga aaaattataa
    10381 tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc caacgaatgc
    10441 agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa agccaagaga
    10501 tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata cgacgaaatc
    10561 atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag gggcaggata
    10621 taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac ccaatgatga
    10681 gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa gaggtggcca
    10741 agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc ctgaacctgc
    10801 taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta caggtgaagc
    10861 aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa ctattgatga
    10921 tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag atgtattaga
    10981 aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga acaagccaag
    11041 taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt atcatgatgc
    11101 cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat ttcttgcccc
    11161 ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag catttggcca
    11221 aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa atccgtggat
    11281 cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta tgcaggatgt
    11341 cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac tggaaattgt
    11401 cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa ataacggtcg
    11461 ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact cagcagagat
    11521 aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga ttgatgaatc
    11581 gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt ctccttggat
    11641 tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta gattttgaat
    11701 taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg gagcctggga
    11761 aaatacagga aatcccctac acggtcttta accgaacaga taaatcagta acagttgaga
    11821 cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga gaatcaaaaa
    11881 gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca gtgggagaaa
    11941 taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg ccagaaaaag
    12001 agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag aacgtttcta
    12061 acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc ttgatttgaa
    12121 atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg gatcaaagcg
    12181 atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat atgttcggct
    12241 tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt tcaataaaaa
    12301 aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca ttagaaggaa
    12361 aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa gaaaaacgct
    12421 ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg aatgaaaagg
    12481 atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata cttgttggag
    12541 tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg aaaaagaaat
    12601 gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat gtacacaaag
    12661 tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg tgaccaaacc
    12721 agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa atgatcctga
    12781 tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga cgattgacca
    12841 agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag tatatgctgc
    12901 attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt tcgtacaagt
    12961 aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa cagaatttat
    13021 tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca aaaacgccgt
    13081 gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg gaactggcgc
    13141 tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag gagcaaatga
    13201 agggattggc acatggattt attcattagg agagaatgtg gaacaaggga gacaatcagt
    13261 ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa cattcacatg
    13321 gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg atttcaggga
    13381 gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc agctcttatt
    13441 catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt ttcaaagatc
    13501 tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg agcagaatca
    13561 attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa ttttaggtta
    13621 tcgctgaaag ccccggaaat acgggcattt cgcaggatat gagaactcaa tttactacca
    13681 atttactact ttcggattga gccgcatata tgctgaacca tactgctaat acccgccacc
    13741 ccaaatacgg cacatcataa attgaatacg acaccgtaaa cgcggcgttc ctctttcccg
    13801 tcatgggaga acaggacgcc gcttttttat gcccaaatag agaaaggagg cgacgccatg
    13861 ctatttactt ccggccctga ccgggcgttt gaacggctga tgcaggaaaa gcccggtttc
    13921 gatcactacg acggcggcgg ggcggacgcg ccggaggatt gcggcacctg ccgattttac
    13981 cgcccggatt ggaaatatca attctgccag tttgcagagt gtccctatca gccgggcaag
    14041 ctgaccgact atgcagcggt caaattcaca gtgaaaggag cgaacaacgc tatggcagtt
    14101 ttccgtgtgg aaaagaacaa aggctatacg gtgatgtcaa atcaccacct gcggaataag
    14161 aacctgacct taaaggccaa gggcctgctg tcgcagatgt tgtccttgcc ggaggattgg
    14221 gactataccc taaaaggact gtcctatatc aaccgggaaa gcatcgacgc gatcaggaca
    14281 gcggtatggg agcttgaaaa agctggatat atccggcgtg agcagggccg tgacgcaaaa
    14341 ggcaaaatgg ccgatatggt ctataccatc tacgaacagc cggtattgga aaacccggta
    14401 ttggaaaatc caacatcgga taacccggtg ttggaaaatc cgacatcgga taatccggcg
    14461 tcggaaaatc caacgcaatt aaataaagaa gtacaaagaa ctgacttatc aaaaaaagaa
    14521 aaaataatta cagatggaca aagtacccat tccattccta tcccatcccc gacccctgcc
    14581 cctatggagg gcgaggcagc ggctacgccg ccggaacgga aaaggacagg aaaggatgag
    14641 gctgtacgga tttatcggga aatcattttg gagaacatcg agtatgccta tctcattcag
    14701 gacaggagca ttgaccgtga acagcttgac gaaatcgtag acctgatgct ggaaaccgtc
    14761 tgcaccgccc gaaagacaat ccgtgtcgcc ggggacgact accctgccga gctggtgaaa
    14821 tccaagtaca tgaagctgac cggcgaacat atccgctttg tccttgactg tctgcgggag
    14881 aacacaacga aagtccgcaa tatcaaacaa tacctgcggg ctatgctgtt caacgccccc
    14941 agcaccattt ccaactacta cacggccctt gtcgcgcacg acatggcaca gcccgattgg
    15001 ggaaagccta aatccggcct gccggactat tcctgcggcg agggcgaaag cctgtaacga
    15061 gaaaaggagg acgaccctat gaagcagggc gcattgattt ttgacgagcg aacagaccgt
    15121 tacgacatcc gctttgatct ggccgactat tacggcggcc tgcattgcgg ggagtgtttc
    15181 gacgtgatga tcggcggcaa atggaggccc acccgcattg aaatggccga aaactggtat
    15241 cttgtcggca tccgggccga cgacctttcc ggcctgcggg tgcggatttg acctatatct
    15301 acctgcggcg tctggtggtt ccgccttaga cgccgctttt gactttctga caaggaggga
    15361 tgcgattgca ggaagaagtt actcaaaaga ccattgccct gtccatgaaa acgggcaagc
    15421 tcacggctca agtgctgcaa gcggcgctga aaaagtacct gcaacaccgg gccaagggca
    15481 aaaacacgct gcaccacggc cagcagagcc taaagcagct taaaaagcat ggggctgatc
    15541 tctccaacat tgagatcacc gaggccaaca ttggagcgtt caagccatgc gccaagaaat
    15601 acggcgtgga ttttacctta cgaaaagata agaccaccca gccgccccac tacgttgtga
    15661 ttttcaaggc caaggatgca gataaccttg atctggcgtt tcgggagttt accgccaaga
    15721 cgctttccaa ggagcagcgg ccctctatcc gcaaggtttt gtccgctgtg aagcaaaagg
    15781 cggcggccca gaccaagcag cgggccaagg agaaaatcaa ggaaaggggg ctggaactgt
    15841 gaagcctgac gtaaagaaat tgctgatcct gaacctcccg tatctgattt ttgtgtacct
    15901 gtttgcgaaa tgcggggagg cgtaccggct ggccgctggt gctgacctgt cggggaagct
    15961 cctgcatttt gcggatggtt ttgcggcggc ttttgccaac ccgctgccaa gcctgcacct
    16021 gtttgacctg tgtatcggta tcgccggtgc cgtgatcgtg cggctggtgg tgtactgcaa
    16081 gagcaagaac gtgaagaaat accgcaaagg catggagtat ggcagcgccc gctgggggac
    16141 ggccaaagac atagcgcctt acatcgaccc caagtttgaa aataatatcc tgctcaccca
    16201 aacggaacgg cttaccatga ccggcaggcc caaagacccc aagacagcgc ggaataaaaa
    16261 cgtgctggtg atcggtggca gcggttcagg gaaaacgaga tttttcgtga aacccaacct
    16321 gatgcaatgc tttccgacca cggactatcc gacttcgttt gtggtgacag atccgaaagg
    16381 cactttggtt ctggaaaccg gcaggatgtt ccagcaggcg ggctaccgca ttaaaatcct
    16441 gaataccatt aacttttcca agtccatgaa gtacaacccc tttgtctaca tccattcgga
    16501 aaaggacgtg ctaaagctgg taaataccct gatttccaac accaagggcg agggggaaaa
    16561 gtcagcggag gatttttggg tgaagtcgga acggttgttt tattcggccc tgatcggcta
    16621 tatctggtac gaggccccag cggaggaaat gaacttctca acgctgctgg aaatgataaa
    16681 cgccagtgaa gcccgcgagg acgacccgga gtttcaaagc cccgtcgatc tcatgtttga
    16741 gcgtttggag cagaaagacc ccgaccattt tgcggtgcgc cagtacaaga aattcctgct
    16801 gtccgcaggc aagacccgtt cttccatcct gatttcatgc ggtgccagac ttgcgccctt
    16861 tgacatccgg gaggtacgcg agctgatgga ggacgacgaa atggagctgg acaccatcgg
    16921 ggacgaaaag acggtgctgt atctcattat gagcgatacg gacacgacct ttaatttcat
    16981 tctggctatg ctccaaagcc agctaatcaa cctgctttgt gaccgggctg atgatgtgta
    17041 cggcgggcgg ctgcctgtcc atgtacgcct gattttggat gaatttgcca acattggcca
    17101 aatacccaat ttcgataaac tcattgccac cattcgaagc cgtgaaatat cggcctctat
    17161 tattttgcag agccagtcgc agttaaaagc gatttacaag gacgccgccg agatcatttc
    17221 ggataactgc gatagtgtgc ttttccttag tgggcgcggc aaaaatgcca aggagatttc
    17281 cgatgcgctg gggaaagaaa cgattgacag tttcaacacc agcgagaacc ggggaagcca
    17341 aacgtcccac ggattgaact atcaaaaatt aggaaaggag ttgatgtccc aagacgaaat
    17401 tgccgtgatg gacggcggca agtgtatctt gcagctacgg ggtgtgcgcc cgtttttatc
    17461 ggagaaattc gatattacca agcatccccg gtacaaatac cttgccgacg ccgacaagaa
    17521 gaataccttt gacgtggaca gatttttgac tatcacgcgc cggaaacggc agcaagtggt
    17581 aacgcaggat gaagtattcg acctgtatga aatcgacctg tcggacgagg acgccgccgc
    17641 tgaataaaca gcggcttttt tcattccaac aacaaaatca aaaaatcatt atgagccgga
    17701 aaccggcaga aagaggtaaa tttatggcat tttttaattc cgcagttact actttgcaga
    17761 ctatcgtgat cggactgggc ggcgctctgt gcgtatgggg cggcatcaat ctgttggagg
    17821 gatacgggca ggacaatccg ggggcaaaat cgcagggtgt caagcagctt gtcgctggcg
    17881 gcggcgtggc cctgatcggt gtcactctgg ttcccctgct gtccgggctg ctgggctgat
    17941 cctggccttt gtccctgtcc aaacccttaa ctaatcgcct ccgcgtcctc ccggccagac
    18001 cgggagggcg cgggaaagga ggtgctaccc gtgattagtg aaatcattga agaatggata
    18061 aaaggcatcc tcatggacgg tatcacgggc aacctgtccg ggctgttcga tacggtaaat
    18121 gccaaagtcg gagaaatcgc cgccgacgtg ggacgaaccc cgcaggcatg gaacagcggt
    18181 attttcaata tgctgcacag cctgtcggaa accgtgatcg tccccatagc cggggccata
    18241 ctcgcccttg tcatgtgcta tgagcttatc cagatgataa cggaaaagaa caatatgcac
    18301 gactttgaca gctccatgtt cttccgctgg atattcaaat ccgcgtttgc aatcctgatc
    18361 gtaacgaaca catggaacat cgtcatgggc gtattcgacg ccacccaaag cgtcgtcaat
    18421 caaagcgccg gtgtgattat cgggcagaca tccattgatt ttaacaccct gataccagac
    18481 cttgaaagcc aattggaggc tatgagcgtg ggcgctcttt tggggctgtg gttccaaacc
    18541 cttgttgtgg gcctcaccat gaatatcctt tccatctgca ttttccttgt gacctatggc
    18601 cgcatggtgg aaatctatgt tgtcacgtcg ctggggccta tcccccttgc cacaatgggc
    18661 aacgcggaat ggcgcggcat gggccaaggg tatctgaaat ccctgctggc gctgggcttc
    18721 caagcctttt taatcatggt tgtggtgggt atctacgcgg tgctgataca gaatatcggt
    18781 tccgcttccg acatatccgg cgcaatctgg ggctgtatgg gctatacggt gctgctgtgc
    18841 ttctgcctgt tcaagaccgg cagtatatca aaggccgtat tcggggcgca ttgatcccaa
    18901 aatccaagca tacacaggag gaacttgttt atttcaacgt acaacatcat ttacgctgac
    18961 ccgccgtggc ggtatgactg caaaaacgta cagggcgcag cggaaaatca ctattccacc
    19021 atgagcattg acgagctgtg cgccctgccg gtggaacggc tggcgtccaa agactgcctg
    19081 ctgtttttgt gggccacatt ccccatgctg ccggaagctc tgcggttaat caaagcgtgg
    19141 ggcttttcat tcaagaccgt ggcttttgta tggctgaaac agaaccgtaa aagccttaca
    19201 tggttttacg ggctgggccg ctggacaagg ggaaacgcgg aaatctgcct gctggcaaca
    19261 cacggcaagc cgaagcgtcg ttctgcctct gtccatcagt ttattatcag cccgattgaa
    19321 caacacagca aaaagccgga tgtgacccgt gaaaaaatcg tagagctggc gggcgatttg
    19381 ccccgcgtgg agctgtttgc cagacagaaa acccccggct gggacgtgtg ggggaacgag
    19441 gtggaatgtg accttatcct gacgtgaaag gaggtgccaa atggcttatg taaccgtccc
    19501 caaggactta acccatgtga aaagcaaggt tgtgtttggg cttaccaaac ggcaactgat
    19561 ctgtttttcc ggggcgctgg tgatcggcgt ccccttatat tttctcaccc gtgactatct
    19621 taccaacagc gcggcggccc tgctcatggt atttgccatg ctgccgggct ttttgcttgc
    19681 catgtacgag cgtcacggcc agccccttga agtggtgatt atgcagatca tccagtgctg
    19741 cttcatccag ccaaaggagc gtccctatca gaccgacaac acctacaccg cccttgtgcg
    19801 gcaataccag atggaacagg aggtaaaggc cattgtccaa aaaggaaatc ccaagagaaa
    19861 ccgaaaaaac aaagctgacc cgcgcccagc gaaaagaaat tgacgcggta atccgtaaat
    19921 ataagggcga cggcaggccc cacacggcgc agcagagcat cccctacgag gtcatgtacc
    19981 cggacggcgt gtgccgggta tcgcccggcg tcttttccaa gtgcattgag tttgcggaca
    20041 tcagctatca gcttgcccag ccggacacgc agacggctat ctttgaaaaa ctgtgcgacc
    20101 tgtataacta tgtggacgcc tccattcata tccagttttc tttcctcaat cggaaagttg
    20161 acccggtgca gtacgccaag agctttgaga tcgcgccgca gggggacgac ttcgacgaca
    20221 tccgggccga gtacaccggc attctgcaaa agcagcttgc aaacggcaac aacggcatgg
    20281 taaagaccaa atacctgaca ttcaccatcg aggccgaaag cgtgaaagcg gcgcgagcca
    20341 gattgaagcg catcggcttt gacctgttag gctatttcaa gagcatgggg gccgtagccc
    20401 atgtgatgga cggctgggaa cggctgaacc tgctgcatgg cgtgtaccac ccggacggcg
    20461 agatattcaa ttttgactgg aagtggctgg ccccgtccgg cctctccacc aaggatttta
    20521 ttgccccgtc ctccctgtgc ttcggcaatg caaagacatt cggcatgggc ggcaaatacg
    20581 gcgcggtaag tttcctgcaa atcctgtccc cggagctgtc ggacgatatg ctggccgact
    20641 tcctcaatac ggaaagcggt gttttagtca atctccatgt gcaggccatt gaacagacca
    20701 aggcgatcaa gaccatcaag cgcaaaatta ccgaccttga cgctatgaag attgcggagc
    20761 agaaaaaggc cgtccgcagc ggttacgata tggacattct tcccagcgac cttgccacct
    20821 acggcgagga cgccaagaag ctgctgacaa agttgcagac ccgcaatgaa cggctgttcc
    20881 agttgacgtt ccttgtcctc aacgtggccg acaccaagca aaagctgaat aacgacgtgt
    20941 tccaagccgc aggcgtggcc caaaagcata actgtcccct tgtccggctg gactaccagc
    21001 aggaacaggg ccttgcaagc agcctgccgc tgggcgtcaa tcagatcaag atacagcgca
    21061 gccttacgac ttccagcgtg gccgtgttcg tcccctttgt gacgcaggag ctttttcagg
    21121 gcggcgcggc catgtattac ggcatcaacg ccaagtcccg caacatgatt atgctggaca
    21181 ggaagcaggc ccgttgcccc aatgccttga agctcggcac ccccggcagc ggcaaatcca
    21241 tgagctgtaa atccgagatc gtcagtgtgt tcttaacgac gcctgacgat atttttattt
    21301 cagacccgga ggccgaatat tacccccttg tcaaacggct ccacggacag gtaatccggc
    21361 tgtcgcctac cagcaaggac tttgtgaacc cgctggacat caacctgaat tattcggagg
    21421 acgataaccc gctggccctg aaatccgact ttgtgctgtc gttctgtgag cttgtcatgg
    21481 gcggcaagaa cgggctggaa gccattgaaa agaccgtgat agaccgggct gtgcgcgtga
    21541 tctaccggcc ctatctggca gacccccgcc cggagaatat gccgatcctg tccgacctcc
    21601 acaaagcgtt gttagatcag catgtcccgg aggctgaccg ggtggcgcag gcccttgacc
    21661 tgtatgtgtc cggctccctg aacgtattta accaccgtac caacgtggac atcggcaacc
    21721 ggcttgtgtc ctttgacatt aaggagctgg gcaagcagct caaaaagctg gggatgctca
    21781 ttgtccaaga ccagatatgg gggcgcgtca cggccaaccg aagccaagga aaggccacat
    21841 ggtattttgc cgacgagttc catttgcttt tgaaagagga acagacggcg gcctattctg
    21901 cggagatttg gaagcggttt aggaaatggg gcggcattcc aacaggtgcc acccagaacg
    21961 tcaaagacct gctttcctcc ccggaaattg aaaatatcct tgaaaacagc gacttcatta
    22021 cgctgctcaa tcaggcgtcc ggggatagaa aaatccttgc ggaacgcttg aatttgtcca
    22081 ccgaacagca gaagtacatc gacaattccg aacccggcga gggccttttg atctttgaaa
    22141 acgtggtgct gccctttacc aaccccatcc cgcacaacac ccagctttac aagattatga
    22201 cgacccgctt aaacgaggtg gcgggagtat gacccggcga cgccacaaag gaggtgaacc
    22261 accatgaaag agctgaaagc caaggcgaaa gtgacgcagg ccatgacccg cgacgggctg
    22321 acgctggaaa atcaggccac cggcgaggtg gagaacattt ccagccggga ggccgaacag
    22381 gatttgtccg cgccggggcc gggcggcacc gccgataagc tgctggaacg ggccgacgcc
    22441 atccatgacg gccataaggc caagaaagcc gcgaagcagg ccggggctgt ggccgccgag
    22501 ggggacgctg ccctgcaccg gccatccgcc cggctccaac tgtcggagga agaacgggcc
    22561 gacccggatt tacagcccta tatccgaaaa tcggacaaac gggccgataa gctggataag
    22621 gcccgcgccg ccatccccaa gaagcgcgtc ccggtcaagg aaaccgtctt tgacgaggca
    22681 agcggcaaag caaagaccgt cctgcacttt gacaaacagg acaaggggcc tccctccctg
    22741 aaacccaatc cggcaggccg tcctgtttcc gaggtttttc tattcggcca cgggaaaatc
    22801 catgaagtcg agcatgaaaa cgtgggtgtg gagggcggcc ataaggggga ggaacttgcc
    22861 gagcggcagg ccggaaaagc catccgcagc accatccggc accacaggct gaaaccctac
    22921 tgggacgcgg aaaaggccga gcgcaggcta ttgtctgcca acgcggagta tttctaccag
    22981 aaatctttgc gggataaccc ggagatcgca caggcggcca gcaaccccat ttcccggttc
    23041 tggcagaaac agcagatcaa gcgccagtac gccaaggccg cccggacagc gggccagacc
    23101 gcaggcgcgg cggcacaggg cgcggccaag acagccgggg cctccacgaa agcggccaag
    23161 gccgcagcca aagaaagtga acgggcggcc tcctttgtgg cccgccactg gaaaggggcg
    23221 ctggccgtcc ttgtgttggg gcttttgatg ctgcttatca tcggcggctt acagtcctgc
    23281 accgccatgt tcggcagcgc cggaagcggc atcacggcgt cctcttactt ttccgaggat
    23341 agcgatatgt tggccgccga ggacgcctac gcggagctgg aagccgagct gcaagactat
    23401 ctggacaact acgaggccac ccatgactat gacgaatacc attttgacct tgacgagatc
    23461 agccatgacc cctatgtgct gatttccatt ctgtcggcgc tccatgacgg gatatttacc
    23521 atcggggagg tacagggtga tttgtccatg ctctttgaca agcaatatat cctgacagaa
    23581 aacgtcgtgg tagaaacccg ttaccgcacc gaaaccgata catggacgga cgcggagggc
    23641 aacacccata cagacaccta ccaagtgccg tatgactatt acatctgcta cgtcacgctg
    23701 gaaaacttcg acctgtccca tgtgccagtc tatatcatgg acgaggaaac gctaagtttc
    23761 tattccctgt atatgtccac gctaggaaac cggcctgatc tgttttccgg cagccagtac
    23821 cccaacgcct ccaccctgac gccgccgacc tactacgacg tacccccgga ggctttggag
    23881 gatgccacct ttgccgccat gctcaaagag gcggaaaaat atatcggtta tccctatgtg
    23941 tggggcggca gctctcccag caccagcttt gattgcagcg gcttcatttc gtgggtaatc
    24001 aatcatagtg gatggaacgt gggacggctg ggggcgcagg gcctatataa catctgcacc
    24061 cctgtttcca gttcgcaggc caagcccggc gaccttgtat ttttcaaggg aacctacgac
    24121 acccccggcg tgtcccacgt cggcctgtat gtgggcaaca atgtgatgct gcattgcggg
    24181 aacccgattt cctacacgaa ccttacttca aactactggc aatcccattt ttacagttac
    24241 gggcgtttac cttaacgcca gaaaggagct ttcaattatg gcaaccaaac ttgaccgcat
    24301 cgaaaaggac atccagaaaa ccaagtctaa gatcgcggag tttcagaaac agcttcggga
    24361 gctggaaacg cagaaaaccg aacaggagaa tttgcagatc atccagcttg tgcgcggcat
    24421 gaacatgaag ccggaggaat ttgccgcgtt cttacgcagc ggcgcaatgc aggccgcccc
    24481 tgccgcaacc ccttaccatg aacaggagga caatgcccat gaagaataag accagacgga
    24541 tttcccgtat tctccccgca cttctggccg tgatgctctg cgtcacggcc tttcccgtta
    24601 ccgcccttgc aggcggcaat gacccggcac cggccccgct gccggaggct acaaccgaac
    24661 ccgctacggg cggtttggag cctgaaacag acgaaaccgg caccgccacg gacgggacag
    24721 aaaccgaggc cgaaaaagat accggccctc tgaccggcaa ggacatcacc gacctgtttt
    24781 ccgcgctgtt cggttccaag gtgagcattg ccgccacgga cgacggcatc cagatcacca
    24841 ccggcaccga caaaaaagaa cctgaacaga ccggcaccgt caccaccaac ggcggcaggc
    24901 tgaatgtccg caccggcgcg gggcttgaca agaccgcgtt cacccagctt cccaatggga
    24961 cgacggttga ggtagtcggc acagacggcg actggattaa aattctcttg ccggagcgca
    25021 tcggctatgt ccattccgac tatatgaccg tcagcgaaaa ggaagtagcg gctaccggcg
    25081 agggcggttt ttccctctcc attgacccgg aggaaattgc ttctctgctg gaactgttca
    25141 atggcggcgg ggccagcgcc gccctgtccc cggacgggaa cctgtcactg attgacgaca
    25201 tcggcagcgc cgcgaaaaat ggcaagcagt ttatcaccgt ggaaaccaaa aacggcaatg
    25261 tgttttatct gattattgac cgggacgacg agggaaaaga aactgtccat ttcttaaatc
    25321 aggtggatga agccgatctg ctgacgctga tggaggacgg ggaaaccgtc acccccgctg
    25381 ctgtctgctc ctgtaccacg aaatgcaagg ccggggctgt gaacacgaat tgcccggtat
    25441 gtaaaaccaa cctgaccgag tgcagcggcc cggagccgca ggaaccccag ccggaggaac
    25501 ccgaagcccc ggaggaagaa cccaagggcg gcgcgggcgg cctgatcgtg ttcctgctgg
    25561 tggcgctggc gggcggcggt gcggcccttt actatttcaa gttcaaaaag cccaaggctg
    25621 aaaccaccgg cagcgacgat ttgggcgagt atgattttgg ggaggacgag gatttggagg
    25681 acgaggaacc cgaaaccgag tttgacccgg aagctgattt tgtgagtgaa caggaggacg
    25741 aggaatgaca tataaacttg taatcgcgga aaaacccagc gtggcccgca gcattgcggg
    25801 cgtgatcggg gccacggaga aacacgacgg ctatttggag gggaacggct atctggtgag
    25861 ctggtgcatc ggacatctgg tgtcctttgc cgacgccgga cgctatgacg agcgtttcaa
    25921 aaaatggcgc tatgaggatt tgcccattct cccggaaaca tggcagtaca tcgtccccga
    25981 cgacaagaaa cagcagtttg acgttctctg ttcgctgatg cagcgccccg acgtgaccgg
    26041 ccttgtgtgc gccaccgacg ccgggcgcga gggggaattg attttccgtt tcgtctacca
    26101 gatggcggga tgccaaaagc cctttgagcg cctttggatt tcctctatgg aggacagtgc
    26161 tattaaggac ggctttgccc acctgaaacc gggcggcgac tatgatccgc tgtatcaatc
    26221 ggccctgtgc cgggccaagg ccgactggct gatcggcatt aacgccaccc ggcttttttc
    26281 cgtcctgtac cacaagaacc tgactgtggg ccgcgtccag acgccgacct taaaaatgct
    26341 ggtggacagg gacgccaaga ttaccgattt caagaaagag aaatatcaca tcgtccatat
    26401 cacggcgggc ggcgcggacg cggtaagcag ccgcttttcc gacgctgccg aggcaaacac
    26461 cgtcaaggcg gcttgtgcaa aagcgcaggc cgtttgcgtt tccgtgacgc gggagaaaaa
    26521 gacggaacag ccgcccaagc tctatgacct taccaccttg cagcgggagg ctaaccggct
    26581 tttcggctat accgccaagc agacccttga ctatgcccag gcactgtatc taaagaagct
    26641 gctgacctat ccgagaacgg atagtcaata tcttaccgac gatatgctgc ccgccgtgga
    26701 aagccttgtg tccggcctgt gggagctggt gccgtttgcc aagggcctga acctttcccc
    26761 gcagtttgac cgcatcctga acagcaaaaa ggtgtccgac caccacgcca ttatccccac
    26821 cgctgaattt gtgaagcagg gctttggcac tctggccgag agtgaaaaaa agctgctgtc
    26881 cctgatctgc tgcaagctct tatgtgccgt ggccgggccg catatctatg aggccgtcac
    26941 cgccacattt acctgtgccg ggcaggagtt taccgccaag ggcaagaccg tcctttcccc
    27001 cggctggaaa gagattgaac gccggttccg ttcttcctta aagaccgacg ccgacgagga
    27061 cgccgaggcg gtgcgggagc tgccggagct ttgcgagggc cagacctttg cagacgtggc
    27121 cgcctctgtc accgagcatt tcaccacgcc gcccaagccc tacaccgaag atacgctttt
    27181 gtccgctatg gaacgggccg gggccgagga tatgccggag gatgccgagc gcaagggatt
    27241 aggcacaccg gccacccgcg ccgccattct ggaaaagctg gtgcagatgg gctttgtcca
    27301 gagaaaaggc aagcagctca ttcccaccaa ggacggcatc aatctggctg tggtgctgcc
    27361 ggaggcgctt acttctcccc agcttactgc cgaatgggaa aaccggctga ccgagatcgc
    27421 caaggggcag gccgatcctg acgaatttat ggacggcatc gagacgcagg cgcgggagct
    27481 ggtgcagacc tattcctgta tcagtgagga aaagcagaaa ttgtttcagg ccgagcgtgt
    27541 tcctatcggg acgtgtcccc gctgtggtga ggctgtctat gagggcaaga aaaactacta
    27601 ctgcggaaac cgggcctgcc agttcgtcat gtggaagaat gaccgatttt ttgaggaacg
    27661 caagaaagcg ttcaccccga agatcgccgc tgcgctgctc aaaaccggca aagtcaaggt
    27721 aaagggcctc tattccgtaa agaccggcaa aacctacgac gggacggtgc ttttggctga
    27781 taccgggggc aagtacgtca actaccgcat tgaacacagg agctaaagga cagtaacaca
    27841 gcaagcatag ggagtgtggc tacactcccg gtaaaatccc ccggccgcgt ccctgccggg
    27901 ccggggattt ttgcttgttg gggaagtccc caacccccta ttttttgaaa cagaacagag
    27961 aaaggagtga ccgcgtgaac gaagtatttt ctatccaagt ccataaccgg cagtttttgg
    28021 aacagggcaa acaaggcgtc tggctggatt tgcccaccac aaccgaaacg ctgcaagccg
    28081 ccctgcggga tgttggcatt actgccgaca atccgcagga tttttttatc aacgggtatt
    28141 cctgcccgga ggacagacac cttgccattc cctatgacat ggtgctggcc gccgacgtgg
    28201 acgagctgaa tttccttgcc gcccggctgg gccagcttga cgccgccgag atcgcagagc
    28261 tgaacgccgc cctgcaaaat ccccgtggcg gctttgagaa tatcggccag atcatcgact
    28321 acaccgaaaa tgtggattac tatgtgcatc ttcccgacgt taggggggat ggacagttgg
    28381 gcgactacta cctgaaccgt tccggcatgg tggatatgcc ggaggaatgg aaagccggga
    28441 ttgatacggc gcagtttgga cgccacattg cccagcagga acaaggcgtt tttacccagt
    28501 acggctatct ggtgagaagc ggcgacgaat ggcagcgcgt ccatgagggc cagccggtgc
    28561 cggaggaata ccgggtgatg cactaccccc agccggaagc tgaccgggag gaaccgcagg
    28621 cccagcagcc ggaggcatcc gaaagccccg acaaagcgcc gcccccggtt atcccgatca
    28681 tgctggacgg catgaacagc aaagaacgca tgaaagtgat tactgaccgt ctggaaaccg
    28741 gcgtacagga gctttttgaa agtagccgct atatggctta cttatccgct atgtccaaga
    28801 tgcacgatta cagtctgaac aatacgctgc tgatcgccat gcagggcggt tcgattgtgg
    28861 cgggctataa caggtggcag gatgaatttg accgccatgt gaagcggggc gagaaaggca
    28921 ttaagatact ggccccggcc ccgttcaagg ttaagcagca gatggagaaa atcgacccgg
    28981 ccacccagca gcccgtgatc ggcgcggacg gcaagcccgt gaccgaagaa caggaagtga
    29041 cggttccggc ctttaaggtg gtgacagtct ttgacatcag ccagaccgag ggcaaggatt
    29101 ttcccatgcc ctatatcagt gaacttaccg gcgacgtgca gcagtatcag gatttctttg
    29161 ccgcgctgga acggacatca ccctatccca taagcattga gccgatttca gacggggcca
    29221 agggccgctg cttctacgca aaacagcgca ttgccatcaa cgagggcatg agcgaattgc
    29281 agaccctaaa gaccgccatc catgagattg cccatgccaa gctccacgcc attgaccggg
    29341 aggcccccgc cgaggaacag gcggcccggc cagaccgcag cacccgcgag gtacaggccg
    29401 aaagcgtggc ctataccgtc tgccagtatt acgggctgga cacgtcggac tattcttttg
    29461 gctatgtggc cgattggagc agcggcaggg agttatccga gctgaaagcc tcccttgaaa
    29521 ccatccgggc cacggccagc gaattgataa ccgagatcga cggccatttt gcccagcttc
    29581 agcaggagcg cgaggcccgg caggaacagg aggccgtcac ggttgacgtt accccggagg
    29641 cagcggcccc ggcgcaggcc gaggaagaac aggccccggc ccttgatccg gcagccgagc
    29701 cgcttgtgac tgtgctatgg agcgaaagcc cccagctaaa ggacggccag caaatgccgt
    29761 tgcatgaggc cgacgcgctg tttgcccgtc tggatgcagc atatccggca gaggcaggct
    29821 atgacaaaac cgcgttccgc attgactgta tctttcaggg cgagccggac agctacgagg
    29881 ggcggcagga ttttggagat agggacggtt ccctgatcga acatatccag agctaccacc
    29941 aattttacgc gcaggacgaa aactggaaaa agcatgtaat cgctacaacc ggcatagagg
    30001 gctgggagca ggacaaggcc catcgggata tgctgttagg cgagtttgtc ccctatatga
    30061 agctgcattg cagccttgcc caaatggaac agacggcgca gggccgctta cagtccgggg
    30121 aaaccctgac gccggaggaa acggcctatt tgggggcgct tctctcttat gtgcaggagt
    30181 gccgccccat gctcaatcag ggccagtatc agttgccgga gccgccgaaa ctggctgact
    30241 tcgaccagag cttgcaggac tacaaagcac aggtacaggc cgagatcgcg caggaggccg
    30301 ccgcagccgg tatgaccgtg gaggaatacg cggcggctgg ctatgaagca ccccaacagg
    30361 acaccttttc catctaccag cttcgaggcg gggaggaaac ccgcgactat cggtttgagc
    30421 catacgagcg tttacaggcc gcagggctgg ccgtggacag tgccaactat gaccttgttt
    30481 atacggcacc gctggataaa agcacgtctt tggaggatat ttaccgcaca ttcaacatcg
    30541 accacccggc agattttagg ggacacagcc tttccgtttc cgacgtggtg gtgctgcacc
    30601 agaatggaca ggacaccgcc cactatgtag acagcttcgg ctatatggaa gtgccggagt
    30661 ttttgcggga aaatcccctg cgtaccgccg aactgtctac ggagcagaac gaaaacatga
    30721 ttgacggcat tatcaacaat acgccgcccg aaccgcagcc ggaggaaccc gaacagctat
    30781 cttttgagga tgtggcagtt gcttccggcc agacacagga ggaacccccg gaggctgcgc
    30841 ccccgcaaac ggctgtccgc tattatccca tcaatgaggg agccgcccgc cgcgccaagg
    30901 aagccatgag cttttctgac taccggcccg gcagcgccac ggcggaatac cggcactatg
    30961 tcgatcaggc cgtggaaatt gcggagcgcc agaaaaagcg tgtggagcca gagtatcacg
    31021 aaaagataga cagcctcctt gacacctacg cccgcaagct ggccgagaac atgaataagg
    31081 gctatgagat taccgcccgc gtcccctctg tcctgattgc cgggccgtcc aatttcccgg
    31141 tacgggcgaa agaaaagcag aacgccgcca gtgaccgcaa catggaggaa tttcagtaca
    31201 ttcagggctt gcttgacaaa atccgcagca ccggcatggg tggtatcagc gccgacgatc
    31261 cgcaggccgt ttccaagctg gaaaagaaac tggaaaagct ggaagcgtcg caggagttga
    31321 tgaaggccgt caatgcctac taccgcaagc atgggacgct ggacggatgc ccccatctga
    31381 ccgaaagagg cattgaaaac ctgaaagcgg atatggcgtc gggctggcac tatgagaaaa
    31441 aaccgttcca gtcctggcag ctctccaaca ataacgccga gatacgccgg ttaaagggcc
    31501 gcattgagga attgacccgc caaaaggagg cggcctatgt gggatgggag tttgacggcg
    31561 gcactgtgga gatcaaccgg gtggccaacc gcctgcaaat cttctttgag gggaagccgg
    31621 atgctgctgt acgcgacgag ctgaaaagca acggtttccg ctggtcgccc aaggccgggg
    31681 catggcagcg gcagttaaac gacaccacaa tccgcgtcgc tgaccgtatc aagtgcatcc
    31741 agcccctttc cggcgaaaag cccagcgcct tacagcttgc ggcccgcagg gagaaagaaa
    31801 agccctctat ccgggcgcag ctcaacgcgg ccaagaccga acaggccaag cagccaccgg
    31861 cgcgggaaaa atcaaaggga ttggaggtag agtaaatgca gaagtttgaa aatgtggacg
    31921 ttctggccgc gctggaacag atcatgcggc agaacaccgc gttttatcaa agtgactttg
    31981 gcattgacaa agacattatc aggcgggatg ccgccagcga tcaggccgca gacaagaccc
    32041 tgctctggat gtcccggccc tccgggacgt actgtttccg ggaacgtgat gttttcctga
    32101 aagacacccg acagtacaac acatggaaat tttacgggga gcagacccgt gacaagatac
    32161 tggcctacgc ggtggagctg accggcacac agggcggcac tatccggggc aatctctatg
    32221 agctggacta tccgcagcat ttccggcacg tcattgaaac cgcccagccg gtgagtgtga
    32281 accggctctt ttatgagcat ggcacccgtg acatcccgga gagccagcgt tttgacggaa
    32341 gccctgaccg ggtattggga aactttttgt actatgaggc ccagccccat gacccggctg
    32401 tgctgcagga ggcgctacgg acggagcagc acagccgggg gcgggccgtc cccggcgatt
    32461 tcaaagtcca cgtcaccgct ttgcatgaca gccggattga aaccgaggcc cggcgtattg
    32521 tggacagcct gaaagatctg tccgcgccta acagccccaa taaaacgcat ttcatggtgg
    32581 agatctcccc gcactttgcg gcgattgctt ccaccaagga taatgaccgg ctctttgcca
    32641 tgctgcccta taaatccctg tgttttaccg ggatgaaagg caggcatggc gtgtatgccg
    32701 tgattaaccg ggacgagcgc cgggatgtga gtattcgcag gccccggccc tctatccgca
    32761 agcagctatc cgacagcaaa caggccgcca gcccaaaaaa ggcggcggcc agaaccaaaa
    32821 agaatgaatt ggaggtataa ggaatgatgc actttaccgt ggaggaagaa aacctgatct
    32881 gtatgtacca caatgcagac cggcgcagaa cgataaacag gctcaccgcc gtcatgccgg
    32941 ttatggatgg ggatatgcgg gcgcaggccc agcagaccct ttccaagctg gaacgcatga
    33001 cggatgccga ctatgacggc cagaagttcc acttcacaga cgaataacga acagcagacg
    33061 ccgggcgcgg cggccatgtg gtcgcttgcg tccggcgtct tttctttttg cctattccta
    33121 cgcatttaga acccggtatt gtcggggata ggatgaagta ttcgcttgcc cctgcaaaca
    33181 tgcgggagaa gccttgcagc gggcggtttt caatccctta aatgcgtagg ttgcagctat
    33241 aattagaccc ctgaaacaga tatagccgcg cagcgggtgt atctgtttca ggggtgtgca
    33301 ggtgatagcc gcaaagcggc gtaagagggt gcagccctct tgccgtctat ctctgttcca
    33361 gcgggaagct cggcaatgct tccagcagtt tcagggaaat gcgctgccgt atatcttcgt
    33421 caatgacttt cttctttttg ccgtttgcct gtctgatctc caccgtggaa agctcgttga
    33481 tgtagtcagc ataatgggcc aacacttttt ccgtggccca cctctcaccg gcaacggcgg
    33541 ctttgatcgt ttcatagtcc agcagctttt cttgtccgtc catcctgata cccccgtcag
    33601 actgattttt tctatgatac catattcgga agcatggaac cagagaaaac ggcgcttttc
    33661 tgctggtaaa ttctacgcat ttagaacagc acttcggcgg ggataggata aaacacccac
    33721 ataccccgct gaccatgccg gaaaagcctt gaaatataag ggcttttaca gctctaagtg
    33781 cgtaggtttc cagtatcttt tccgctggcg ctcggcctct ttgcggcggc gcatccgttt
    33841 ggagcaggcc gcgcagtatt tggcccggtt agacttcggc gtgaacgtgg ccccgcactc
    33901 acagcagcgt ttcacttcgt cccggtcttt ggtgatctcg gcatacaggg ccgcatccag
    33961 cgggaggaca gcggccttaa accagcggca cagaagcgaa taggaaatgc tctgtacgca
    34021 aatacattct tcgccgtcgt ccagcaacag gcagttgccg tcacaatagt tgcagcactc
    34081 atgcgtcagc ttccgggcct tgcgatattg agggtaattc attcgtggaa tactcatagc
    34141 tcttgcgtcc attcctttcc cggcgtcata cgcaaaatgc cgtccacatt ctgcttgatg
    34201 atgccgtatt cctgcatctg ctttttcgct tcgccgtact tctcatacag ccgttccttt
    34261 tcctcggcca atcgcttgta ctcggctttc agggcgtaca gatcggggag ctttttcaca
    34321 ccctgtccct ttagggcctt tgccgcagct tcatacaaaa tcaacgtgct ttcatgctca
    34381 cgccggaatt ttgccttatc cgctgcattg cggtattcca tagccacggg ccagagctgc
    34441 ttgtaggtag aaatattctt tatcagcacc gccatatccg caagccggtg ttccatagct
    34501 ttgagggtag cggcggcctc gtcgctggcg gcggtgatct cggccacttt gctttccaaa
    34561 tccgggtagg tgtctatccc atgttcagtc aggaaattca aagtctttgc ggcctgcttc
    34621 aaattgtgga gcttcgccca cctctcatag cctgccgact gctgggcctt aatgttgttt
    34681 tccagatcaa tccgcaaaga aatccccttg cgctctttgg gcgctctggt gataacgcgg
    34741 cctttgatac gctctttgat ggcttcctcg gtgtaggctt cccccaatgt cttgcagcgg
    34801 gtaaagcgtt cctgtccggg agcgcggaaa gaaataaact ttcccggctt gatctcatag
    34861 ccctgcgcct gcatgagccg caggaaatca tcaaaatctt ttgcctgcgg gatagcggcg
    34921 tcaatggctg ctttgagctt cgctttccag ctcgtccctt tccgctgggc gtcccactcg
    34981 gcatagctct tgcccttact ctggccgggc ttcaccacgg acaatccgtt ttccttgcac
    35041 agcctgtcgc tggtgcgccg gatataggca tagctctgtt tgttggaaat gtacttgcgc
    35101 tgatttacca tatccacggc acagaaaata atgtgattgt ggacgtggcc cttatcaata
    35161 tgggtagtca gcacataggg atatttccct tgcagcactt cctcggcaag ctgcttcccg
    35221 atctcatggg cctgttccgg cgtggcttcg ccgggggcaa aggattgtat caggtggtgg
    35281 cccagattat tgcccttttg gtatgcctgc gccagcagca tttcaaattc aatgtccgct
    35341 gtttcataag agcaggcaaa ggaggccacc agcagcttgc cgtcagtttt gtccgggttc
    35401 tcgatgtagt caagggcttt tttcagcgtt gacttgatag gatgtatctt tgtaactgcc
    35461 atatctgcgc cagcgccccc tttatatcct cgatgtcctg tgcgtacacc ggccccatac
    35521 cgttcagcct ctttgcaatc tgattgacgt tgacgccgat cttctgtatc tcggcggtct
    35581 gcgcccggat gtcgctggtg tccatgttga cgatatagcc gtcaatgagc atcttccggg
    35641 cgtaggctga aaagttcttc gtatgcagca gcgccatttt ctgttcaatc atagcccgtt
    35701 cctccggcgt cacagggacg cgcagcacga tttttcgctt tctgtttgcc atgcttcacc
    35761 gtcctttcca ttcaggggtt tggggatttc cccaacaagc aaaacggacg cggcccggca
    35821 gggacggggc aggccgtttt tccgggagtg gctacactcc ctatgcttgc tacgttactg
    35881 tttctccact aacaagacgt ttccaaagcc ccaaactacc cgcaaaatag ccctctggaa
    35941 agaaaaaagt gcaaaaaaat agaagctacg ctgtgcagct cccgctaaaa gtaaaacaga
    36001 ggatgccagt tacacatcct ccgtttcatt cttcttcggc ttttcaatta gtatggcgct
    36061 gtcaatcgcg ccctcgatga tcggcaggta ttcttcgggg cagagtttca gcttgtggct
    36121 gaccctttgc cgcagttcgc tttcctcccg catgatctcc gggttaaagt agcgttccac
    36181 aggaagcccg caggctttaa taagctgtat gacaaccggc agactgggaa tggccccgtc
    36241 gttttcgatg ttggcaagat agcgccagtc aatatttatt ttctctgcca ttgctttgcg
    36301 cgaaacgcgc tttgcttgcc gggccgcttt cacatccgcg ccgaaagttt caaagccggg
    36361 acaatcttca atattcgcca tatcgcacct cccacttaca ttgtatattt catactctgc
    36421 ccgtggaatg ttggtatatg cacctttctt aaagtttata tttcacattt tgtttctttt
    36481 attcttcgag caaaagaatt ataatgtacc ctgtggaggt gtactatgga ctacatgacg
    36541 ttgaaagagg caggcgagaa atggggtgtg cccccacgat ggataaacta ctattgtgct
    36601 gcgggacgca tccccggcgc tgtaaagatg gcgacgatct ggctcattcc caaaactgcg
    36661 gaaaagccga ttgacaggcg tacaaagaaa ggaaaggcgg ttactgaata gtataatgaa
    36721 accggcgcat tttggatatg gttttgaaga gagagaaagc atggtatttt gtcacggaaa
    36781 aactataaga tgatagatgg caggctgctc cagaccgata aaaagtattc tcatctgaaa
    36841 cagaaagaaa agatttacac atggatgtat gaagaagcga aactttatca tgacagaaat
    36901 ggaaaatgcc cgaaacaaag acgaggaagc tgctgctgta tcagccgtat atggtagaat
    36961 tgaaaaggcc ggcatctgga ttctttatgg tgaagtctcc agacattgga taactgctgg
    37021 tctaatgcgc ctttgagtat ggggtgattg attttaatac cggggaggta cggctttttg
    37081 aggaaaatat tctgggattt acatttggcg attattacat ttcctgtaac tgcaaggaca
    37141 cggatgatga agatatgagg aatatagact tgctgatttt ctactgtctt gaacggtatt
    37201 aggactgaat ctgctgcgcg gagtgcggca agagcgaccg gagggagtac acacaggccc
    37261 tgcctgtgtg aaaagccata ccgcaaggtg cattcgcaaa aaagaggccg cgcattttgc
    37321 tgaggcttgg ggtgtcccca acaagcaaaa cgagaacgcc gcaggcgggc aagtggtgga
    37381 tcagaatatc cgtaaaggat attctgatcc attgcttgcc agtattatcg ggatgttttg
    37441 acaaccgctg taatcgaaac atcccaaaca ttaagaaggt gttaattatt attatcttga
    37501 tttaattcgt ttaaaccaat aatatagata catacaccat gaaaagaagc gagtatccca
    37561 atgacggatg attaaaatcc gaaaagaaat cgctttgaaa ctacagggaa actacagact
    37621 gttatgttat cttcttaaaa tggagggatt tttatgtcga tacgaattct acttgtcgag
    37681 gatgatgatc atatctgtaa tacagtaagg gcgtttctgg ctgaggcagg atatcaggtg
    37741 gatgcctgca cagatggaaa tgaggcatac accaagtttt acgaaaacac ttatcaactg
    37801 gttattcttg atattatgct gcccggtatg aacgggcatg aacttttgcg tgaatttcgt
    37861 gcgaaaaatg atactcccat tctgatgatg acagccctgt cggatgacga aaaccaaatc
    37921 cgggcgtttg atctagaggc agacgactat gtaacaaagc cattcaagat gcagatttta
    37981 ctaaagcggg tggaagccct gctgcggcgc agcggtgcgc tggcaaagga aatccgtgtc
    38041 ggcaggctga cacttctgcc ggaggatttc acggtacttt gtgacggtac agagctgccc
    38101 ctgacacgaa aagaatttga aatccttttg ctgctggtgc agaacaaagg cagaacctta
    38161 acgcatgaaa tcattttgtc acgtatatgg ggatatgact ttgagagtga tggcagcaca
    38221 gtccacactc atatcaaaaa tttgcgggcc aagctgccgg aaaatatcat caaaaccatt
    38281 cgcggtgtag gataccgatt ggaggaatca taataatgga aagaaaaggg attttcatta
    38341 aggttttttc ctatacgatc attgtcctgc tactgcttgt cggtgtaaca gcaacactgt
    38401 ttgcacagca atttgtgtct tatttcagag tgatggaatt acagcaaaca gtaaaatcct
    38461 atcagccatt ggtggaactg attcagaata gcgataggct tgatattcaa gaggtggcag
    38521 ggctgtttca ctacaataac caatcctttg agttttatat tgaagataaa gagggaagcg
    38581 tactctatgc cacaccaaat gccaatacat caaatagttt tagacccgac tttctttatg
    38641 tggtacatag agatgataat atttcgattg ttgctcaaag caaggcaggt gtgggattgc
    38701 tttatcaagg gctgacaatt cggggaattg ttatgattgc gataatggtt gtattcagcc
    38761 ttttatgcgc gtatatcttt gcgcggcaaa tgacaacgcc gatcaaagcc ttagcggaca
    38821 gtgcgaataa aatggcaaac ctgaaagatg taccgccgcc gctggagcga aaggatgagc
    38881 ttggcgcact ggctcatgat atgcattcca tgtatgtcag gctgaaagaa accatcgcaa
    38941 ggctggagga tgaaatcgca agggaacatg agttggagga aacacagcga tatttctttg
    39001 cggcagcttc tcatgagcta aaaacgccca tcgcggctac aagcgttctg ttggagggaa
    39061 tgcttgaaaa tatcggtgac tacaaagatc attctaagta tctgcgcgaa tgcatcaaaa
    39121 tgatggatag gcagggcaaa atcatttccg aaatactgga gcttgtcagc ctgaatgatg
    39181 ggagaatcgt acccatagct gaaccgttgg acatagggcg cacggttgcc gagttgctgc
    39241 ccgattttca aaccttggca gaggcaaaca accagcggtt cgtcacagat attccagccg
    39301 ggcaaattgt cctgtccgat ccgaggctgc tccaaaaggc actatccaat gtcatattga
    39361 atgcagttca gaacacgccg cagggaggcg aggtacggat atggagtgag cctggtgctg
    39421 aaaaatgccg cctttttgtt ttgaacatgg gcgttcacat tgatgatact gcgcttccaa
    39481 ggctgttcac cccattctat cgcattgatc aggcgcgaag cagaaaaagt gggcgaaacg
    39541 gtttaggact tgccatcgta caaaaaacgc tggatgccat gagcctccag tatgcgctgg
    39601 aaaacacctc ggatggcgtt ttgttctggc tggatttacc gctcacatca acattgtaaa
    39661 tatttaaaac ttaaatgatt ttgaccgaca ggtataaccc tgccggtctt tttttgctgc
    39721 tacaggaaaa ctacagactg gctacaggga aagtacagat gcgcttgcca taataacaat
    39781 cgtaccagcc acaaatcgta gttttattgc aaaggaggca ttcgatcaaa tggaaaaaag
    39841 caactatcat tccaatgcgg atcatcacaa acggcatatg aaacaatccg tggaaaagcg
    39901 ggcttttcta tgtgcgttca ttatttcgtt cacagtctgc acgctgtttt tggggcggag
    39961 actggcttcc gtattggagg caacacagat accgcccatc cctgcaactc atacaggcag
    40021 cagcactgac gtagtggaga atttggagga aaacgctctt gccaccgcca aagaacaggg
    40081 agatgaacag gaatggagcc tgattttagt gaacaggcag aaccccatcc ccgcacagta
    40141 cgatgtggag cttgagcaac tatcaaatgg tgagcggata gatattcgga tttctcccta
    40201 tcttcaagat ttgtttgatg ccgcaagaac tgatggagtt tacccgattg tcgcatccgg
    40261 ataccgaaca acagaaaaac agcaagaaat tatggatgaa aaaattgccg aatataaggc
    40321 gaaaggctac acctctgcac aggctaaagc ggaagcagaa acttgggtgg ccgtgccggg
    40381 aacgagcgag catcagcttg gtcttgctgt ggatatcaat gcggacggaa ttcattcaac
    40441 aggcaacgag gtttatagat ggctggatga aaacagctat cgttttggtt ttattcgtcg
    40501 ctacccacca gacaagacag agataaccgg tgtgagcaac gagccgtggc attaccgata
    40561 tgttggcatc gaagctgcca cagagatgta caaccaaggg gtttgccttg aggaatattt
    40621 aaaaccagaa aaatgagaaa aggatgttat ggacagaaaa agattgacac agcgtttccc
    40681 gttcctgctt ccaatgagac gggcgcagag aaaaatgtgc ttttatgcgg gaatgagatt
    40741 tgacggctgt cgctatgcgc agacgatagg ggaaaaatcg ctttcccatt tgctctttga
    40801 aacggattgc gcattataca accacaatac cggatttgac atgatatacc aggaaaacaa
    40861 ggtgttcaac ttaaagctgg cagcaaagac cttaaacggc ttattgataa gaccggggga
    40921 aaccttttct ttctggtggc tggtacgcca tgcggacaaa gataccccct ataaagacgg
    40981 ccttacggtg accaatggta aactcaccac catgtcgggc ggcggtatgt gccagatgag
    41041 caatttacta ttttggatgt tcctgcatac gccattgaca attatccagc gcagaggtca
    41101 cgaagtaaag gagtttccag agccaaacag tgacgagatt aaaggggtgg atgcaaccat
    41161 ttcagagggc tggattgatt taaaagtgcg aaacgatacc gactgcacct accaaatatg
    41221 agtgacctta gatgatgaga aaatcatcgg tcaggtgtcc gccgacaaag agccccaagc
    41281 attatacaaa attacaaatg gcagtatcca gtatgtccgt gaaagtggcg ggatttatga
    41341 atatgcccag gttaaacgga tgcaagttgc cttaggtacc ggggaaataa tagattgcaa
    41401 gctgctttat acaaacaaat gcaaaatctg ttatcccctc ccggaaagtg tggatatt
//
LOCUS       DACDXE010000019        40668 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.019, whole genome shotgun sequence.
ACCESSION   DACDXE010000019 DACDXE010000000
VERSION     DACDXE010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.019"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(294..494)
                     /locus_tag="IWM23_001010"
     CDS             complement(294..494)
                     /locus_tag="IWM23_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9883670.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(799..2031)
                     /locus_tag="IWM23_001011"
     CDS             complement(799..2031)
                     /locus_tag="IWM23_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9883671.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2285..2854
                     /locus_tag="IWM23_001012"
     CDS             2285..2854
                     /locus_tag="IWM23_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9883672.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3032..3472)
                     /locus_tag="IWM23_001013"
     CDS             complement(3032..3472)
                     /locus_tag="IWM23_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAP9883673.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3630..4394
                     /gene="map"
                     /locus_tag="IWM23_001014"
     CDS             3630..4394
                     /gene="map"
                     /locus_tag="IWM23_001014"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAP9883674.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4426..5349
                     /locus_tag="IWM23_001015"
     CDS             4426..5349
                     /locus_tag="IWM23_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAP9883675.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5425..6564
                     /locus_tag="IWM23_001016"
     CDS             5425..6564
                     /locus_tag="IWM23_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAP9883676.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6557..7357
                     /locus_tag="IWM23_001017"
     CDS             6557..7357
                     /locus_tag="IWM23_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9883677.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7357..8184
                     /locus_tag="IWM23_001018"
     CDS             7357..8184
                     /locus_tag="IWM23_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9883678.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8162..8896
                     /locus_tag="IWM23_001019"
     CDS             8162..8896
                     /locus_tag="IWM23_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9883679.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8996..9862
                     /gene="rfbA"
                     /locus_tag="IWM23_001020"
     CDS             8996..9862
                     /gene="rfbA"
                     /locus_tag="IWM23_001020"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAP9883680.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9876..10448
                     /gene="rfbC"
                     /locus_tag="IWM23_001021"
     CDS             9876..10448
                     /gene="rfbC"
                     /locus_tag="IWM23_001021"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAP9883681.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10470..11498
                     /gene="rfbB"
                     /locus_tag="IWM23_001022"
     CDS             10470..11498
                     /gene="rfbB"
                     /locus_tag="IWM23_001022"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAP9883682.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11596..12447
                     /gene="rfbD"
                     /locus_tag="IWM23_001023"
     CDS             11596..12447
                     /gene="rfbD"
                     /locus_tag="IWM23_001023"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAP9883683.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12482..14515
                     /locus_tag="IWM23_001024"
     CDS             12482..14515
                     /locus_tag="IWM23_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883684.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14559..15839
                     /locus_tag="IWM23_001025"
     CDS             14559..15839
                     /locus_tag="IWM23_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAP9883685.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16049..16855
                     /locus_tag="IWM23_001026"
     CDS             16049..16855
                     /locus_tag="IWM23_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883686.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            16867..18087
                     /locus_tag="IWM23_001027"
     CDS             16867..18087
                     /locus_tag="IWM23_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883687.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18077..19663
                     /locus_tag="IWM23_001028"
     CDS             18077..19663
                     /locus_tag="IWM23_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9883688.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19702..21840
                     /locus_tag="IWM23_001029"
     CDS             19702..21840
                     /locus_tag="IWM23_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9883689.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(22208..23191)
                     /locus_tag="IWM23_001030"
     CDS             complement(22208..23191)
                     /locus_tag="IWM23_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HAP9883690.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            23569..24960
                     /locus_tag="IWM23_001031"
     CDS             23569..24960
                     /locus_tag="IWM23_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9883691.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            24976..26016
                     /locus_tag="IWM23_001032"
     CDS             24976..26016
                     /locus_tag="IWM23_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9883692.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            26035..27120
                     /locus_tag="IWM23_001033"
     CDS             26035..27120
                     /locus_tag="IWM23_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9883693.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            27153..28115
                     /locus_tag="IWM23_001034"
     CDS             27153..28115
                     /locus_tag="IWM23_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-1,6-N-acetylglucosaminyltransferase"
                     /protein_id="HAP9883694.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            28108..29535
                     /locus_tag="IWM23_001035"
     CDS             28108..29535
                     /locus_tag="IWM23_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HAP9883695.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            29537..30607
                     /locus_tag="IWM23_001036"
     CDS             29537..30607
                     /locus_tag="IWM23_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883696.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            30594..32015
                     /locus_tag="IWM23_001037"
     CDS             30594..32015
                     /locus_tag="IWM23_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9883697.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            32028..33035
                     /locus_tag="IWM23_001038"
     CDS             32028..33035
                     /locus_tag="IWM23_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9883698.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            33047..34051
                     /gene="neuB"
                     /locus_tag="IWM23_001039"
     CDS             33047..34051
                     /gene="neuB"
                     /locus_tag="IWM23_001039"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HAP9883699.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            34048..35196
                     /gene="neuC"
                     /locus_tag="IWM23_001040"
     CDS             34048..35196
                     /gene="neuC"
                     /locus_tag="IWM23_001040"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HAP9883700.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            35169..35846
                     /locus_tag="IWM23_001041"
     CDS             35169..35846
                     /locus_tag="IWM23_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAP9883701.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            35836..36978
                     /locus_tag="IWM23_001042"
     CDS             35836..36978
                     /locus_tag="IWM23_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HAP9883702.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            36991..37968
                     /locus_tag="IWM23_001043"
     CDS             36991..37968
                     /locus_tag="IWM23_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9883703.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            37968..38876
                     /locus_tag="IWM23_001044"
     CDS             37968..38876
                     /locus_tag="IWM23_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9883704.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            38877..39629
                     /locus_tag="IWM23_001045"
     CDS             38877..39629
                     /locus_tag="IWM23_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9883705.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            39634..40581
                     /locus_tag="IWM23_001046"
     CDS             39634..40581
                     /locus_tag="IWM23_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAP9883706.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        13584 a         6205 c         7925 g        12954 t
ORIGIN      
        1 agggtcaaat ttaaaccata attgtgtttt tccttccatc agcataaaag ttcacctcaa
       61 aaagagtata cgaacgaatg ttctttttgt gaaggcggaa tttaatatac tgaataatag
      121 aaaacaagta cttgtgccaa attatgtgcc aaaaaaattc gaattcaaca aaatgtaaac
      181 aaatagaaaa ccttctatat taagatttcc tgcaataaca aacacaacag aagacgtgta
      241 atagttagtc aggaacgtac aaataacccc tgtatccttt gcggtacagg gggttatttt
      301 gtataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt
      361 atatttactc cttgataaag taaaatagat gcatgcgtat gtcttagtcc atcaataaac
      421 agtcgaagag aaaataggga aaaattgatt actaaagaaa aacttactag gtcgacggtg
      481 tagttgattt tcatacttca atagattcga attaaacgat ttcgattggc ttataccagt
      541 cctatccaac gaaagatata tcgttcttaa ctctagcggt ataacttgaa gctttacgta
      601 aagccaatgg gaatgaactg gagatgactt tttattattt ttagtaatag ctgatgggtt
      661 aatacttaca actttccttt tcaaagtacc atataaaaaa acggtggggt tgtttagcga
      721 aataaaggat ctaagccaat agcttagatc ctttattcta tgctttatat tattttttgc
      781 atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta ctgtagagcc
      841 atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat ctacgtgggt
      901 ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg taccgccttt
      961 cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt catcaaataa
     1021 tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta caagtgcgac
     1081 ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga catcttctcc
     1141 ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca tacccgagat
     1201 gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta ctcgatgact
     1261 gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt ctccacgagc
     1321 attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa tgatatctgt
     1381 accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt ctgctttttt
     1441 tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat agacacgagt
     1501 cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca gttctgggaa
     1561 gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga ctttatttgc
     1621 ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat aagacgctac
     1681 tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc tgatcctgct
     1741 tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta cattctcaat
     1801 acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag tccattgaac
     1861 gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag caaggggtgc
     1921 ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta cgccagtttc
     1981 tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca taataaaaat
     2041 tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta tgattcaaac
     2101 aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc atgatatatg
     2161 ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca tatcaaaaaa
     2221 acatgttaca atgaaattaa tcaatggtgg aagcgttata aaaaaattaa ggagattaaa
     2281 aaatatggca agaaaaaaga cgattactag agaacagatt ttggcagctg cttatgaagt
     2341 agctgccacc gatggattta cacgatttac tgcgagaaac atcgcgaaca aaatgaattg
     2401 ttccacgcaa ccgatttatc ttgagtttaa aaatatggat gacttgagac aagctctttt
     2461 tgagcagatc tatgatcatt tacaatatga agtgttcact gttgagcata cagggaatac
     2521 gattatcgat cttgctttta attatatcca ttttgcaaga agagagaaga agttgtatcg
     2581 ttcactgtac ttagaagaat atggtggagg aaaagagatg cagaatttct cttatactta
     2641 ctttgtgaat acagtgaaac gcgatccagc ttatgtgaat ctttcagaag agcagatcga
     2701 atctttgtat aatgggacat ggatcatagc tactgggatc gcagctttga tgtcttcaag
     2761 catcatccat ccaactgatg aacagattga aaagatgatc caagacagta tagatgctat
     2821 cttgaaattc aaagaaccaa tcgaaatcga ttgaatatac tcaaaagttc cacaaatcag
     2881 taggtaacaa gagatgtgtc aaaataagga aaagttcgtt tttcttacag cgtatgcatt
     2941 tttaactccc gaagaactct tccatgaatg caatgaataa aagagccata cagattgaat
     3001 aaatctggat ggctctttta ttgcaagtat attaaatttt tgcgactagt tctttagcga
     3061 aagcttctaa attttcgata tcttcttctt cagcattcag atccacttta acattttcgg
     3121 ctccttttga tgcgcctgtt tttgcaaaag cagcatcgaa atcatccacc gatttgcaga
     3181 attcatcgta aaaagtatca ccagatccgc agacaccgta aatttttccc aatagatcca
     3241 attcttgcaa gtcttcataa aagtctacga tctcatcagg cagatcgcca tcatcgtatg
     3301 tataagtagc tactacgcaa atatctgcct cctcgaattc atctgcatct acttgggtac
     3361 attcgttgat ttcgacttcg atatccagat tttcaaatgc ttctgcaaca atatcggcga
     3421 tttcttcagt gtttcctgtc atgctagcat atacgatttt tgctaaagcc atgatgtgcc
     3481 tcctcgttta taattattta acaattatga attataccaa aacatcattg aaaagaaaat
     3541 aaaacctaaa gacacctggg gaatgtcatt tggctaaatg tatgccgcta tgttaaaatg
     3601 agagggattt gtaaaaagga gacgaaaata tgattacttt aaaatcagca cgtgaaattg
     3661 aaatgatggc agaatctgga gctttattag cagatgtaca taaaaatctg cgtgatttta
     3721 taaaaccagg aatcacaagt tgggatattg aggtatttgt cagaaactat atcgagagtc
     3781 atggcggaat tgctgcgcaa attggctttg aagggtatga atatgcgact tgtatcagta
     3841 taaatgatga aatttgtcat ggattccctc gtaaaaagcc tttgaaaaac ggtgatttgg
     3901 taaaagtcga tatgtgtatc gatttgaaag gcggcatttc tgattcttgt tgggcctatg
     3961 tagttggaga atctacacct gagatcgacc gattgatgga agtaacgaag aaagctttgt
     4021 acatcggtat tgagcaagca caagtaggga atcggattgg tgacatcggt catgctatcc
     4081 aaacttatgt agaaggcgaa aatctagcga tcgttcgtga ttttattggt catggcgttg
     4141 gtcctacgat tcatgaagaa ccagcagttc ctcattacgg agaagctgga aaagggttgc
     4201 ggttgaaaga aggcatggtt atcacgatcg aacctatggt taataccgga acttggaaaa
     4261 tgaaaatgga tcctaacggc tggactgcgt atacaagaga cggcggactt tcttgccaat
     4321 atgaacatac attggctatt actaaagatg gtccaagaat tttgacatca caaggagaag
     4381 aaggcactta ttgatctatc cagtcaatag gtacgggaga ggtacatgaa agtttggaac
     4441 aaaataaaac aaaatgaaaa tctgatgcgc tttattgaga cgactcaaaa gcgcatcact
     4501 gattcagaaa tgagtacgac ctctgtcgtc gttgcttatt atttactctt gtctttattt
     4561 ccattgatta ttgcttttgg taatatttta ccgtttctcc acatcgatca agagactgta
     4621 ctgacctatt tacgagaagt gattcctgag accgtttacc agtttatcgg tcctgcaatc
     4681 aaagaccttt tgacacaaag ttcaggaggc ttgttatccg tttctgcatt agcggcttta
     4741 tggtctgcaa gtcaaagtat caatgctttg caaattgcga tgaataaagc gtatggcgta
     4801 gaaaatagaa aaaatttttt gatcgtccgt ttcttctcac taattgtcat tgtcttgttc
     4861 atgattgcta ttagtggggt aactatcgtt cttgggttgg gacagctgat tttggatgct
     4921 attcagccaa tcgtacaaat acccgtgaac tttatcgatc agttccaaac tttgaagtgg
     4981 ccaatcacat tggtagcttt atttgtcatc atgttcttga tttatttaat tgtccctaat
     5041 gctcagttga agttgaagac gatcattccg ggcacgatct tcgctactgt cggctggatg
     5101 ctgttatcac aagtgtttag tatctatgct aggtattttg caacgaggat cagcggttat
     5161 cagatcatag gaagttttat tgtactcatg ctttggctta attttgctgc tacgatcatc
     5221 attttgggcg gaatcatcaa tgcagttgtt caagaatacg tgacgggtca tcaaatcaaa
     5281 aaacgtcgag atccaagccg aaaatggttc aataaattga aaactaaatt ttctcataaa
     5341 aaaaagtaga aacaagagtt cttattgtaa atttatatca gtaatttata ataattatga
     5401 ctcttaatta aagtaggtgc ccatatgctt gtttctttta gtatggttat aagatttttt
     5461 ctgacaattt tattatcact gatattaaca cctgtattca aaatcatttc agtacaaaca
     5521 ggtatggtcg acaaaccaaa tgaaagaaga ataaataaag ttccaatgcc ttcagcgggc
     5581 ggactgccta tctttatttc atttgttata tcgactttat tcttattccg taaaattatc
     5641 cctcaatcgt atattgtacc gatcttgatt gcttctttag taatcatagt cacaggagtg
     5701 ctcgatgata aatatgagct gacacctaaa caaaaaagta tagggatctt gattgcttct
     5761 cttattattt attttgtagc agatatacgt attgattcct tcacgcttcc ttttattgga
     5821 tatatccaac taggctggct tagttttccc gtaacgattt tttggatctt cggtatcacg
     5881 aatgcggtca atctgattga cggtttggat gggctggcgg cagggatttc attgatcggt
     5941 ttaattacga taggaatcat tggatatttt ttcctccatg cttctacagt gtatattcca
     6001 gttgttattt tctgtcttgt agcaagtatt atcggttttt ttccttacaa tttttatccg
     6061 gcaaagattt atttaggcga tacaggagct ctattcctag ggtttatgat ggccgtcctt
     6121 tcactacaag ggctgaagaa cgtgactttt gtttcatcga tttctttgct gatcgtcatg
     6181 ggagtgccgg taacagacac gttttttgct atcattagga gaaaagcaaa ccgtgtttct
     6241 ttttctacag cagataaaaa acatcttcat caccgactaa tcagtttagg ctttactcat
     6301 aaaggtgctg tcctgacgat ttatgcgatg gctttgatgt tttctttcac agctatggtc
     6361 atgaattata ctggaagaat cggaacgatc gtattgatta ttgccatgtt gtttgctgca
     6421 attttattat ttgaattgat tggtctgatc aatgagaaac atcagtttat tttaaaaaca
     6481 ttgcgatttt tagggaataa agagtaccgt acgcaagtaa aacagaaata tttgaagaaa
     6541 tggaaccgtc atatagatga ataacaaatt aaaccagccg atccttatta gtattgtgac
     6601 ggcaaacagt gataaaattt ttcggacact agataatttt atttcaactg taaaagatga
     6661 aacagcagta aaaatccgaa tttttgacaa caattcactt cccgagtaca aaaaaagact
     6721 aacagagtat aggaaatatc cctacatcga tatctatttt catgatgaaa attccggatt
     6781 tggttatgga cataatcaca atctgatagc tagcgattct cgctatggca ttatttgtaa
     6841 cccagatatt ttagtggatg aagagacagt tacagcattg gtcttgttat tgaaagaaca
     6901 tccggaatgt gcgatgttag cacctaagat tcttaatgaa gatggaacaa ctcagcacct
     6961 catccgtgag cagctggctg tttttgatta cgttttgcgc tttgtgcctt tcaaatgggt
     7021 caaaaaaaag tttgacaaac gactggctgt gtttgaatgc cgaggattgc ctactgatgc
     7081 taatagttat gtacggatga tctcaggtag ttttatggta gctgatattg ataaatttaa
     7141 agagataaag gggtttgatg agcgtttctt catgtacttt gaagacaacg acttgtgttt
     7201 gagttttgaa aaagcaggtt acaagttatt gtacacaccc ttttatgcag ttatccattt
     7261 ttacgggaag ggtgcgcatc gaaatgcaaa actgtttcgc gtgtttttga cttctatgat
     7321 gaaatttttc aataagtggg ggtggcgttt tttctgatga aaccagaaat attagtagtc
     7381 gttgtgcttt atcagcaact attcagtcaa tctccagctt atggaccatt aagcaaagca
     7441 cttagcgaaa agcaaattca gcttattatt tacgataata gtcctcaaag gcagcaaaat
     7501 gagctgtttg aaaaagaaaa tatcgtttat catcatgatc cgcaaaatcc tggtttagcg
     7561 gtggcttata attttgcgtt gtctcaagca gatgaaggaa cacgttgtct cgtcactctg
     7621 gatcaggata cgaggctagt cgacgattat tttgaactat tgcgtaaagt aacctttaca
     7681 gatgaatgtg ttgctgcagt tccgatgatt ttttctggga gccgacaagt ttctccccta
     7741 tattcagatc gttatatcaa tcgtacagct caagcagtag aagttgaagg cccaacagct
     7801 gaacgaatca tggcaatcaa ttcaggtgtt gcctggtctg tcaaatttct aaaaaagatt
     7861 ggcgggttta atcctgcttt ttctttagat tttttggatc attggctgtt ttggaaagtg
     7921 gatcagttgg aaaaaactgt ggagatcctt cctgtacgaa tggaacatga tctttcggtt
     7981 ttagactatg aaaaagtttc aagtaatcga taccgttcta ttttacaagc agaaaatcga
     8041 ttttatcatg aatatgatca aaggcattta tccagtcacc gacgacaatt attgttaaga
     8101 acagctaaac agttcttgac cgtcaaaaac cggcatatct ggcggatgac tttgcgctca
     8161 tatgttaaga attggaaggt gtgaattcgt gcgttcggtt tgtatagcta cctttaatgg
     8221 aggcagatat ttaagagagc agttagatag tattttatct cagctttcag cagaagatga
     8281 agtagtcatt tctgatgatg ggtcagtaga tgatacatgg gatatattaa ctgaatacgc
     8341 agaaaaagat aagcgaatcc agcttttttc ggggcctcgt caaggattga tcgctaattt
     8401 tggttatgcg atccagcaga cgaaaggaga tctaatcttt ttggcagacc aagatgatgt
     8461 ttggttggag acaaaagtga aaaagatcac tgactatttt gagcagaatc cggatcagct
     8521 agtagtagtt tctgatttaa tcattgtgga cagcgagctg caagagatcc atgattcgta
     8581 tttttcgttt cgaaattcca aagaaggatg gcttaataat gtgataagaa gtcactatat
     8641 tggtgctggt atggctttcc ggtcttcttt aaaacccgat attttgccga tcccgtcaga
     8701 agtgccgatg catgatatgt ggattggttt gctgggagga aagaagactg ggttcctaag
     8761 agaaccgtta actctgtacc gaagacatga tttaaacgcg agtgagattg aaacacattc
     8821 taaattacgg caaaaaattt cttggcgtgt tcatctgatt acggcgttga tcaaacgaaa
     8881 atggtttaaa cgttgataaa agatgtttaa atccttaata tttccatgac aaatataaca
     8941 attgatgaga taataacaaa gtgaaatttg aaatcaaaaa ggagagcaga tatccatgaa
     9001 gggaatcatt cttgctgggg gcagcggaac acgtctgtac cctttaacaa aggcaacatc
     9061 gaaacaattg atgccgattt atgacaaacc aatgatttat tatccaatgt ctactctgat
     9121 gttggcggga atcaaagaaa ttctgattat ttcaacgcca caagatacac cacgtttcaa
     9181 ggaattattt ggaaacggaa atgatttagg actgcagatc gaatatgcag tacaagaaag
     9241 tccagatgga ttggcacaag cgtttatcat tggagaagaa tttattggag atgatagtgt
     9301 ctgcttggta ttaggggaca atatttacta tggtggcggt ctgtctaaaa tgttgcaacg
     9361 agcagcttca aaagatacag gcgcgacagt atttggctat catgtcaatg atccagaacg
     9421 ctttggtgta gtagaatttg atgagaatat gcaagctttg tcgattgaag aaaagcctgc
     9481 tcagccaaaa tcaaattacg ctgtaactgg cctttatttt tatgacaatg aggtaatttc
     9541 tatcgctaaa ggtatcaaac cttcagaacg tggagaacta gagattacgg atgtcaataa
     9601 agcatatttg gataaaggaa aactatccgt agaacttatg ggacgcggat ttgcttggct
     9661 tgatacagga acacatgaat cattgcttga agcatctacc tttatcgaaa caattgaaaa
     9721 acgtcagaac ttaaaagtgg cttgtttgga agaaattgct taccgtatgg gctacatcga
     9781 taaggaacaa ttgctggccc ttgcacagcc attgaaaaaa aatcaatacg gtcaatattt
     9841 attgaatttg gccgctgaat aaggagtcct tacagatgaa agtaacagat acaaaattaa
     9901 cagatgtcaa aatcgtagaa atggacgtct ttggcgatca tcgcggtttc ttcacagaaa
     9961 gttattcaaa agcaaaattt gcagaacatg gattgaaata tgactttgtt caagacaacc
    10021 attcactatc agctgaagct ggtgtccttc gggggcttca tttccaaaaa ggagaagctg
    10081 cacagacaaa attgattcgg gtagtcactg gagcagtttt agatgtagta gtggatatcc
    10141 gcaaaggtag cccgacttat ggtcaatggg acggatttat cttatctgaa cataatcatc
    10201 gacagttgct tgtacctaaa ggatatgccc atggattcgt tactttgaca ccaaatgtga
    10261 acttccttta taagtgtgat aattactata atgcagaagc agatgggggc atcgcatttg
    10321 atgatccaga tttagcgatt gattggccaa ttgatccatc aagagcgatt atgtcggaaa
    10381 aagatcaccg tcatccgaca ttaaaagaat ttgaagcaga aaatccattt gtttacggag
    10441 aaatctaaga attaaggaga aattttctta tgaaaaacat cattgttacc ggaggtgccg
    10501 gattcatcgg ctctaatttc gttcattacg tggtaaacaa tcatccagaa gtgcatgtca
    10561 ctgttttgga taaactgact tatgcaggaa acaaagagaa cttggccgga cttcctagcg
    10621 atcgcgtaga attggtcgta ggagatgttg cagatgcaga attagtggac cgtctagtaa
    10681 aagaagcgga cgcagttgtt cattacgcgg cagaatccca taacgataat tcattaaaag
    10741 atcctttccc atttgtacaa acgaatatca ttggtacgta tacactttta gaagcttgcc
    10801 gcaagtatga tgtacgctat catcacgtat ctactgacga ggtttacggc gatcttccat
    10861 tacgtgaaga cctgccagga catggggaag gcgaaggaga aaaattcact gcggaaacac
    10921 catataatcc ttcaagtcca tattcttcta caaaagctgg ttcagatctg ttagtaaaag
    10981 catgggtgcg ttcttttggc ttacgtgcaa ccatttcaaa ctgttcaaac aactatggtc
    11041 cttatcagca tattgaaaaa tttattcctc gccaagtaac gagtatctta agcggtattc
    11101 gtccaaaatt atatggcgaa ggaaagaatg ttcgtgactg gatccatacg aatgatcatt
    11161 cttcagcagt gtggctgatt ttgacgaaag gcagaattgg cgaaacctat ctgatcggtg
    11221 cagacggtga agaagataat aagaccgtga tggaattgat cttagaaatg atgggccaac
    11281 caaaagacgc atacgaccat gtaaacgacc gcgcagggca cgatttgcgt tatgcaatcg
    11341 attcgacaaa attgcgagaa gagctaggtt ggcagcctga gtttacgaac ttccgtgatg
    11401 ggttggctga cacgatcaaa tggtacgaag aacatgaaga ctggtggaaa aaagaaaaag
    11461 aggccgttga agcagcatat gccaaaaatg gacaataata cagaggaaat ttcactgtat
    11521 taatggaaaa acacatgtga ggcaggcgct cgcctgtctc ttttttaaaa taaaaatcaa
    11581 ggagcggata accatatgtt tttattaact ggaggaaatg gacaattagg tacagaattg
    11641 cgccatttat tagatgaaaa aaatctgcca tatgtttcaa cagatgcaaa agacttggat
    11701 atcacagatg ctgagaaaac aatggcttat attacagaat taaaacccga tgttattttc
    11761 cattgcgcag cttacacagc agtagataaa gcagaagaag aagcaaaaga attagatgag
    11821 aaaatcaatg tcgatggcac aagaaatgta gcacttgcag caaaagctgt gaatgcacaa
    11881 ttcgtctata tcagtacaga ctatgttttc gatggaaaaa acaaaaatgt agaatacgaa
    11941 gtagacgacc aaacgaatcc actaaatgaa tatggtcgta cgaaattatt aggcgaaaaa
    12001 gcagtagaag agattttaga agactattat atcatacgaa cttcttgggt tttcgggatc
    12061 tatggacaca atttcgtctt cacgatgcaa cgcctagcgg aaacccgcga caaactgact
    12121 gtagtaaatg accagtttgg gcgaccaact tggacacgta ctttggcaga atttatggta
    12181 tatgtcatcg aagaaaaagc accatttggt atttatcatt tgtcgaacga aaatagctgt
    12241 tcttggtatg agttcgcaaa agaaatattg aaagatacag atgtagaagt tgcacctgtt
    12301 acgtcagaag aatttccgca aaaagcaaca cgtccgcagt attcagtcat gagcctgaaa
    12361 aagacagaag cgttaggttt tgtcatacca acttggcaag aagcacttgc gcaaatgctg
    12421 gaagctgcca aaaaaatgaa taagtaattt cctttttatc aaatagaatt tggagcatga
    12481 tatggaaaaa caaacaagaa gtgcacgacg aaaagaaaag ccaaaagcga atcaggcaga
    12541 ttcgaatttg gtgcttgacc tgtttctaaa actccgctat cttattggcg ttttagtcat
    12601 cgtggtcatc gttactttta atttaaacgg aagttcactt ggtgtttggg ataaatatgt
    12661 gtcgcaaaga gacgatggga aaaaaactga tgtgattttt ggacagaatc gggaagtgcg
    12721 gtcagacgaa tggctggtgc agacaccttt ttacctctca caagctgaag aggacttttc
    12781 gttggtgaac gaggattatt cattaaacgg acaaaacatg atcattgcgt ataattctcc
    12841 tgtaaaggat attacggtta tcggaaaacc attcaactgg ggattctttt tcctcggaag
    12901 agatcgagga ctttcctttt attgggcgat gaaattagtc gtcatggtct tgcttggatt
    12961 cgaagtactg atgatattaa cgaagaggaa aaaagctcta tcgctaatcg gtgcatttgc
    13021 gctgacattt tctccagcag tccagtggtg gttcatgcaa catgtgggcg atttgatttt
    13081 ctttactttg ggactgatga ttgcttttta tcattatttc tacaatcatg ataaaaaatg
    13141 gctacgaacg ttgatgatgc ttttggtggt tattttcggt attgggttta ttttagtgat
    13201 ttacccagcc catcaagtga tgctggcgta cttgctgatt ttttattttg ttggcctgct
    13261 tatttactat agcaagaaag tttcatggga tctgttggat gtagtgttga ttggaggggc
    13321 agttttattt attggcggag tcatggttca tttctggcta acgtcaaaag acgcattgtt
    13381 agcttctttg aataccttat atccaggaaa acgggtaagt actggaggaa agtggacgat
    13441 tggcgaattt ttttctttcc ttacaaattg gaaaattcct tttgaagata tcaactattc
    13501 gaataactct gaagtggccc tgttctatca cttttttcca actgtattcc tagcaagtcc
    13561 atttgtgtta tttggcaaaa aagatagcga acaaaagtta ctgggacgta ttttgatgat
    13621 tttttgtctg ttttctatct tttggattac cgttgggttg ccaaaatggc tagcagaaat
    13681 cactctgctg tcttatgttc ctccattaag agccatcctt acttttagtt ttgcggcttg
    13741 tttattatcg atctggttta tcgcttatat ttggagagaa gatgttattc ctatttggta
    13801 taaattcatt ttgttggcag taaatgcatg gtcttatttc tatgcgatta cgcattcgaa
    13861 aatggataat tatttttcgg attttgaaac catcgcagtt gctgtgttgg gaacagtgat
    13921 tttagctttt gcattatttg gctggagacg cttgttttac ctcgcttttg tcgccttagt
    13981 tatcgtatct ggctttttcg tcaatccagt cgtagaaggt acaggagcga tttttgaaaa
    14041 aacactggca aaagaaatcc aaaaaatcga tcaacaagat cctggtaaag tttggttgtc
    14101 tgaagatgaa ttatataact tcaccccaac attgggagtg aaatccttca atacagtacg
    14161 cttttatcca gatatagctg catggaagaa aatcgatccg aaagaagaat acgagaagta
    14221 ttataatcgg tatgctcata caagagcttt tatcgctaag gaagatacaa agttcatttt
    14281 agatcgtcca gatatgtttt cggtcacgtt gaattttgaa gacgcagaaa aattagggat
    14341 cgactatgtg atcagtaaac gaccattaac tgattacaac caattatatg aagcgcagtt
    14401 cacgcagcta tatggaccgg ataaagacgg ttatatgatt tttaaagtag aatatccgga
    14461 ttatccagat gtaccacaaa cacaaactca ggaaaatcag ctagtcgtac agtaataggc
    14521 tagctgagaa taatcctgaa atattagtag aaaggagagt gaagataatt caagcaatcg
    14581 tacaaaaatt tgtcaacaaa atgagtgtcg gtttcttgct tttgttgctg acgctagtct
    14641 tttttgtttg gacagcaggg gtaggttttc cagcaacttt gtttatatat aacaatagtt
    14701 cattacttat cgcttttagt gtggctattt tgctcatttt aaatgtttat aaagcaaaag
    14761 gcgtcgattt tctttatcta ggtgttgcgc ttgccctttt cattttcttt tcttactttc
    14821 gggaattaag aaatgagtct ttatttggag acttactgtt tcctttggtt attttatttt
    14881 gtttagtaat taaatggatc gagtggacaa aatgggatcg cggtatttat ctgattgtct
    14941 ttacggtctt cctcggaatc acgttatatc gtgtatttac agagatccaa gtaccagaag
    15001 gacaaagcat ctgggctcca ggcaattcgc tttctgatat ttggatcaat acgaatacaa
    15061 tcggaagttc tttgatgact cttgctttat tgatcagtgg atttgccagt tcttttgaac
    15121 gatggtatgt tcgaatcatc ggtgggatag cagtcgttgc agccttttta ggtatttggg
    15181 tctgccaatc tagaggtgca ttagtcgcgt tgattgtttt tgcgatattg gatcttttac
    15241 ctaaatcgtt gatgcgtgtc attcgtgcgc catttattgc ctacacggtt acgattcttt
    15301 tagctttgcc gatttcttat ctggcggctg tctctgaaaa agtcaatttg tttaccgatc
    15361 gagaagatat ttggcataaa ttctatcaga cgctaggaga aaaatctgaa caagttttac
    15421 taggaatgaa aacatttatc ttccagcgag gaaaccaatt tttaggaaac cacaatagtt
    15481 ataattctat tttgaatatc tatggattga taggatttgg gattgctgct ttgctgctta
    15541 tcctttttat tggtcgctta acgttgaaag cagatttatc caacgggcag atgactttta
    15601 tctgggcctt ttttgcggtg atgatgcagt catttatgga ggatacattg acttcatacc
    15661 catgggttcc gattatttat atcctgttgg ctatggccgt tcatcgatat gatgaaccag
    15721 aagttgcgga ggaattctat gttatggaag aggcatatga cgatatgact tatgatgata
    15781 catcttatga ggaacaacca gatagcgctc attcttccag gatccaacgc tatcattgat
    15841 aggcacaaaa tacgagagag gctgtgacaa ccgtcgcagc cttttttggt ttattgcaat
    15901 tgtctagcat cttctgtaaa atagaaacgt tagagaaaat cttgtatcat agaaaattaa
    15961 gaaagaaata agaacgcagc tcggtaccaa aaatatggta aaatggcact tgactgtaat
    16021 tcactgaaaa tcgcttggag gaacacaaat gtttaaagat ttgtatttat ttatgaagga
    16081 catttatcag aacagaaaat tattgctaca gttttcgatc aatgacttca aatcaagata
    16141 tgcaggatcg tttttaggaa tcttatgggc gtttatcaat ccgctattta ctgtattgat
    16201 ttattggctg gtatttggtg ttggactgaa agcacaaatg atcgatgcaa aatacccatt
    16261 tatcgtatat ttagttaccg ggatgattcc ctggttcttc ttctcagatg catttgcatc
    16321 aactacggtt gtttttcggg aatatactta tttagtaaag aaagtcgtat tcaatattcg
    16381 tatcttgccc acgacgaaaa tcctatccaa tctgtacacc catttattct tcattctgat
    16441 tggcatagct gtcgcgctta taaatggtat atatccttca gcaatgtctt tacagctgat
    16501 ttattattta ttttgcatgt gtgctttctt gacaggattg acttggctga cggcatcgat
    16561 ccaaccattt ttacctgata tcatgcaatt aattacgatc gtgctgcagt tgatcatgtg
    16621 gacattaccg atattatgga gccctaatca gtttgattca ggtcttgtaa gcatattgaa
    16681 attaaatccg ctgtactatg ttgttcaagg ttatcgtgag tcatttttaa gtgaagcttg
    16741 gttctgggag catggaacat atactttgta tttctgggtt ttcacgttag taatgttcgt
    16801 aatcggctct gcagtgttcc gtagattgaa accacatttt tcagatgtgc tgtaaggaga
    16861 atagctatga cagaatatgc aattagacta aaagatatca caaaatctta taatatgtac
    16921 gcaaaacctt cggatcgatt tcgtgaagcc ctcaatcctt ttaagaaatc ctatcatgat
    16981 gtattttatg cgttgaaaaa cgtgaatgta gaaataaaaa aaggtgagat gatcggtttc
    17041 gtgggagaaa acggatcggg aaaatctaca atgttgaaaa tcattacggg agtactgaca
    17101 ccaacatccg gcacgatgga aatcgaagga aaaatctcag ctttgctaga actaggatca
    17161 ggattcaatc ctgagtattc tggctatgaa aatatctatt taaatggaat ggttctagga
    17221 ttttcaagag aagaagtcga tgaaatggtc gatgatatca ttgaatttgc tgacatcgga
    17281 gaccatattt atcaacctgt aaaaacttat tcaagcggga tgttcgttcg tctagctttt
    17341 gctgtagcaa tcaatgtgaa tcccgatatt ttgattgtgg atgaagcatt ggcagtaggc
    17401 gatcttgaat ttcaactaaa atgtatggaa aaatttacag aaatcaagaa ctcgggaaag
    17461 acgattttat tcgtttctca tgatatcagt tcgattcgtc gtttttgtga tcgttcctat
    17521 tggttgaaaa acggagaagt cgtcgaatat ggcgatacga tggaagtaac gacgaattat
    17581 gaaaactttt taaagaaaaa atcagttaaa acagtggatc ggaataaaaa ccaagtagaa
    17641 cggttcgccg ctccagatat cgtcgacgtg acgaaagcgg aattgttgga taaagacaaa
    17701 aatccggtgg atatgctgga acaaggagaa aaactatacg ttaaagtaac ttatgaagta
    17761 aaagatgata caatcaaaaa tccggtttta ggaattgcgc tacgaacagt cgacaatttt
    17821 tatgtatgcg gacttaatac tctactagat ggtatcaaga ttccttggaa aaaaggagag
    17881 aatatctttt atctggagta tgaaaagtta ggtttgttag ctggtgaata ttattttgac
    17941 gttgcggtct ttgaagaaaa tgcaacggtt cctttggtat ataagacaaa gtacatgaac
    18001 ttgtttgtca gcggttctta tatcggagaa ggcatcgtgg tcttagacca taaatgggaa
    18061 gaagggactc atagcaatga aatatgattt tgaaatggac ctagatgaac aaagcagtgt
    18121 gggaaaaatt gccgcacaga tcaaaccagg aagtaaagtt ttagagtttg gtcccggaaa
    18181 cggccgatta acgaaacatt taatcggagc aaaacaatgt gaagtgagca ttgttgagtt
    18241 agataaggaa ttgttcgatt ttgtcagcga atttgctcaa gatggatttt atggtgatat
    18301 cgaaagtttt gagtgggcaa actattatgc tggacagaca tttgattacg tcttgtttgc
    18361 ggatgtttta gaacatttgg tagatcctgg aaaaacattg aagaaagtca gagaattttt
    18421 gaatgaagaa ggagagatcc tgattacatt ccctaacttg gcgcataatt ctgtcatgat
    18481 cgatttgttc aataaccaat tgccgtgggc ttcttatggt ctcctagatg aaacccataa
    18541 ttctttttat actcatgatg gcttccaaaa agtttttgaa aaagcgggat tattcattaa
    18601 tattgaagat tatttgtact tagctgtagg tgatacagaa ttaaaatcaa cttatgaaga
    18661 attgccggaa gctgtacgct atgatttcaa aatgcgacca tttggtgaag tctaccaata
    18721 ctttttctca ttgatgaaac atccagtagc acaaagttcc attgcagagc cacaaaattc
    18781 taattatgta aaagtgttag aagtaaccca gcaaacgaaa caagatgaaa caatccaaca
    18841 aataccattc aataatttca caggggaaaa tgagacacta agtttccccg tatcagaaac
    18901 aacagaaaga atggtgtttc gcttcgcgca acaacctagc tttatcgaat tcagcgcaga
    18961 agctgccgga gaaaaactaa catttatcga ttcgaatgct gtagtcaaaa cagtaaatga
    19021 ttgttatttg tttgatggga aagaattacc agagttcgtt ctaacggata tttctgggaa
    19081 agaagtgact atccattgtc actatcgctt tatcggggaa ttgacaccaa caatgaaaga
    19141 attgttggaa acgattcgtc ccatggctga agtcacaaag cagctttctg agaaaaatga
    19201 cgaattagaa agagaaaatc atcatcttct tgaggaaaat acaagactag accatacgtt
    19261 aaaaacaacc acaaatcgct attgcacatt gttggaaagt gatgaatgga caataaaaca
    19321 tagactagga cgacgtaaga aagaaacttc gaaaaaaatc caagaaaaag aactttctat
    19381 atgtattgac gagaaaatct gggatgcaga aacaaaaata ttaaaaatca tcggatgggg
    19441 aatcgctaat tcggatcgcc agcccttgtc ctataagtta tcagctgacc aatcgccatt
    19501 tttcgaagca attcctgttt cacgagaaga agtcaatcaa gcagaacagc tcgcaaaagg
    19561 aacagaagcg ggatttgagt tacgaatcct ttgcgagcaa gaacgttcat tcttagtgga
    19621 agcagtagct caaaatggcc aatcatggat cattgaaatg taaataatga ttaacttata
    19681 aagataagga gtaaacgaat cgtgcaaaaa aaagaaattg ccgtatttat cgatcaaatc
    19741 actcgtgaac gggccactgg cgatttgctt attgtgggct gggcaattga tgaagtgacc
    19801 aaagaaatcc ctacgataaa ggtagaaaaa gaaaatgtga ttgctgaagc aacacatgtc
    19861 gtaagattgg atatcaatca tttatataat cttgatgtta agacccaaag cggtttcaag
    19921 gtacgtttat cagggaaaat gagaggaaaa gccatcttag acttccaaac agcgagacat
    19981 caaaatggca tagctgtgaa attaaacggt aaataccctt atgatgacgg cattgaatct
    20041 tcatgggaaa gaaaaaaaag attactgaaa aaagggataa attatgcaag gactcatggc
    20101 gtaaaaaaag cgatccgtcg ggtcaaattg gaattgaagc caggatcgat cgactatgca
    20161 gagtggatca gtcgtcatga gaaaccagat ttgaaagagc aaaggaaaga agcacaagga
    20221 tttgcgtacc gtccattgat ttccatactt atgcctgtct ataacgttga aatcaaatgg
    20281 ctcgaaaaat gcattgattc ggtactggat caaacgtatg atcattggga actatgtatc
    20341 agtgatgatg cttctactga tccagctatc cgaaagtgtc tggaatccta tcaagcaaaa
    20401 gatgatagaa tcaaagtcgt tttccgaaaa gaaaatggac acatcagtct ggcaactaat
    20461 tctgcattgg aaatagcaga aggagaattt atagcactct tagataatga tgatgaatta
    20521 ccgccatttg cattatatga agtggcaaaa gtgctaaacg tacatccaga actggatgtg
    20581 atctacagtg acgaagataa aatcgatgca gatggcaatc gctttgatcc gcatttcaaa
    20641 gcagactggt cgcctgatac attgatggga aataattata tctctcactt aggcgtatat
    20701 cgaacaagca ttgttaaaga acttggcggt ttccgtaaag gctatgaagg ttctcaagat
    20761 tatgatttgg tccttcgtgt gacagaacaa atccctgcag atcatattta ccacattgac
    20821 cgagtcttgt atcattggcg gacgatccca ggatcaactg caagtaatgg cgaagccaaa
    20881 agttatatct atgattccgg agtgaaagca ttgacagatg ccctgtctag aagaaacatc
    20941 aaaggaagcg tacgtcctgg cagaatttct ggattttatg agattgctta tgacgtcttg
    21001 caagaggatt tggtcagtgt gatcattcca acgaagaatg gctatgaaga tctaaaaact
    21061 tgtgtggatt cgatcatcga aaaaacgtct tattcaaatt atgaaatcat tattgcggat
    21121 aacggaagta cagatccaaa aatgcaagaa ttatttgcag aatacaagca tcagctgaaa
    21181 gaccgcttta tcgtagaatt gattgacatt ccattcaatt attctcgaat taacaattta
    21241 gcagcagaaa aagcaaacgg caaatacttt ttattcttga ataacgatac agaagtgatt
    21301 gagccagatt ggatgacggt gatggtttct tatgcacagt ttgatcgaat cggctgtgta
    21361 ggagcaaaac tgttttatcc agatgataca acgcagcatg caggagtatt attaggaatc
    21421 ggtggtgttg cagggcatgc attgaataac tatgatcgaa cacattgtgg ttatttcggc
    21481 cgattggtca ttgacgtcaa ttacttagca gtcactgcag cttgtatgat ggtcaaggca
    21541 gctgacttca atgctgttaa cgggtttgat gaaacattag aagtagcatt caacgatgtt
    21601 gacttgtgct tgaaagttta tgaactgggt cgttacaatg tctatgcaca tcaagcagaa
    21661 ttgtaccatt tcgaatctaa atcaagaggt tatgaagata ctccagaaaa acaaagacgt
    21721 tttgctggtg aaatcaaaaa aatgcaagat aaatggccag catatattgc gcatgatcca
    21781 ttttacaatg ataatttaac aaaagacgga atcggcgact tttcgttgag accagattaa
    21841 actataataa aaagctgtta tgttgtgatt cgctcgcaca taacggcttt ttatagtttt
    21901 ttgtcaaaaa aaggcagaag gtgccgactc aaaaaaataa gtcgaggttg ggacagaagt
    21961 gtttaactcc gagaaataag aagaaattca cgaaaattgc ttttcaaatt tttgtgaatt
    22021 tcggcttatt taagaagaag ttgcttctgc tcccaccgtt tattcgtttt taaagaatga
    22081 gacaaaagtg tttcttactt ttgtcccacg ctcttattca tgctcagagc gccaatagat
    22141 acaaaatcat agccaaagtt tcacgaaccc tagctgtcaa agaatcgata ttttatttta
    22201 atataggcta ttttaaaata aatggtttaa ggtattcctc tacaaaagcc actctatcaa
    22261 ttatcatggg tttaaaataa gaagtgatgg agataaccag attgttctta gcgtcaatat
    22321 aaagaacatt tccactatta ccaattgctg aatatatttc gttctcagaa tctaataccc
    22381 accataaata gccatatctc atattttgta agtcttctaa agcactggtt ctaggtttga
    22441 acatctcgtt tatccacatg ggtggtacga tttgttttcc tttatacatc cctttattta
    22501 ggcataatac accaattttt gccagatcac ttgccgataa acacaaacca tatcctgccg
    22561 tcccgatatt tagtggatca caaaaccaga cattggtttt aggagactta tccattgtaa
    22621 actgcttatg ttcttcagct gtttcagcat aataattgac atggtttggt atccctatag
    22681 gatcaaatag atacttattg gcaaaatcaa cagttgttag cttacttgtc tgatacaaca
    22741 agccagttaa tatgtgtaaa cagacagttt gatagttgaa ttcgccggtt ataccttttc
    22801 ttcctcccaa taaatctagt gatgtttttg tccaatcttc acttgaacag acttttgtcc
    22861 atggatcgcc tttacatttg tagggtgctt tcattgttag caagtgcttg attgtaatag
    22921 cgtaaatagt tttctctccg cgtttcactt tatactctgg aaaataatct aatactttat
    22981 catcgatact tttgattaat ccttgctcta aagcaatgcc aatcagcaaa gatacaatac
    23041 ttttcgttgc tgacatgata tgacaagtat cgtctttttt ataaccattc cattcatctg
    23101 tataaacttc taccccatcc ttatatgcga caatttgaca tatatttggt tgtcgaaaac
    23161 caataaaatc atgcaactct tgtttattca taagatatta cctcactttc ttctataagc
    23221 ataatgtcat taaaaatcga tttcaatttt ttcgattctt cacgtaatac aggtattctt
    23281 atggactaga cagcgctttg ggaagatgga ttctctgaaa aatataaagg ttcactttat
    23341 gagtggataa aaaaagcagc aaatacttcg acttctttaa tttaccaaca ttagtgaaaa
    23401 tcgattcatc tggagatttg taatttagag tccttgtctt gattagaggt gtataaagct
    23461 ttttattaga catttttaaa ttccattaca atttgcttat aaactaggtt tttaaaggtg
    23521 actatggtaa gataatagag atattatttt gtgattagga gtgtatatat gaataaaaat
    23581 ggagaatgga gtgctgcacg acggatattc attatattaa tggatgttat tgtatacaat
    23641 gcagcaatat atagtagttt cttactgaag tttcgtggag agatcccatt acgaaattta
    23701 gaaactttcc aacactcagc aattattatt tcagtgattt ttattgcgtt gaacattttg
    23761 ttgggtgcat atgtttttta taatcgaatg atcagtgaca tcgtgtttgt cacagtcatc
    23821 ggtcaggtat tgatgacact agggatcatg gtagtgactt ttgcgggccg ctggtttgct
    23881 tttccacgtc ttgttatctt actttcattt gttctcggta cgcttttctt aagtatctat
    23941 cgtattctcg tatataaact ttatttacgc gtaagtcgag ataaaaaggt cgtcattatt
    24001 gggttagaaa aggatgtcgc accagcaatc caaaattttg gttcaaagaa aaacaacaaa
    24061 cataaagtcg aagcagtcat tcttgataac tattatgcaa acatcaaaaa tatgattaaa
    24121 gacatcgata ttgtctatct tgcttctcat atcgaagaaa ctgaaaaaat caaaatctat
    24181 caattagtta ctaaaaatga aaaaaaattg tttttgaata caacatttga aaacttgaca
    24241 ttgatcaatc caaatattat gaatttcgaa gatgaaagta ttattgaagc atctggattt
    24301 agaatccaac ctgaatatga actattcaaa cgtttgtttg atatcatcgt ttcacttgtt
    24361 ttattagtta ttgcttcacc atttatgtta ttcacagcaa ttttagtcaa agtgacatca
    24421 ccgggaccaa tcatctataa acaagtgcgg atcacaaaaa atcaaaagga attttcaatc
    24481 tataaattcc gctcgatgac cgccacagcc gaagcaaaat ctggaccagt tttagctaag
    24541 agcaatgatg cacgagtaac gcctgtaggt aaatttattc gagctgtccg ctttgatgaa
    24601 ttaccacaaa tcttcaatgt gctgaaaggc gatatgtcga tcgtaggacc acgtcctgaa
    24661 cgttctttct tcgtcgatca gttcaatgca gaaaaccctt attactactt acggcataat
    24721 gttcgtgctg gtatcactgg gtatgcacaa gtctatggaa aatatgtctc tgattacaac
    24781 agtaaattac gttttgattt actttatatc aaaaaatatt cattgatgct tgatttaaag
    24841 atcttgctgc aaaccatcaa gatcctattt gataaaatgt cctctcaagg acttgaagaa
    24901 gacgaaaatg tgatctcatt ggcagaaatt gcttttccga atgaaaaaat ctttcgctag
    24961 ttgggaggcg taaaaatgaa caatccgaaa ttcagcataa tcgtaccagt atacaatgca
    25021 gaagcaacta ttggtcgaac gattgatatt ctaaaagaat tggattatga agagtacgaa
    25081 gtcgttttag taaatgatgg atcaacagat caaacacagg aaatcattca agaactgatt
    25141 gaaaatgatg aacgttttga attgattact acgaccaatc aagggcccgg attagcaaga
    25201 aacgaaggaa taagacagtc aaaaggagaa tatctcttat tcttcgatgc agatgacacg
    25261 ccagtaaaaa cgatcttgtc agattatgat catttgctag aaaagagtcc aaaagcggat
    25321 cttgttgtta gttcgtttgt ttttcgtaca atggacgagg gaagaatcgt ttccgaaaaa
    25381 gaaaacttag ttacagaatg tcactatcta actcacgaag agtttataaa agacatgtat
    25441 cagttaatga atgaacagtt gatgtatgtt gtatggaaca aatgctatcg tagcgatatt
    25501 ctgaaaaata accaaattct atttaaaact tataatagtt gtgaggatcg aatatttaac
    25561 ttacattatt atgaatactg tcatcaagtg atcatgaacc caaaagtaga atatatttat
    25621 gaatttgaag gtgggaaggg gatcactaat caataccgac ctaacaagtt cgaaacattt
    25681 aaagaattct attcattagc aaatactgta acagacgata ttaataaaga cggtatggcg
    25741 gcgcttcttt taaaagggac aacttcagtt attttttcta tttatagtac ctctgcaaga
    25801 acttcaaaag agaagaaaga cgaagcaaag cagatattga gtgatccagt gatccgagaa
    25861 gccaagcaaa ttgctaagac ggattcttca gcaaaaaaaa taacaaagtt gctttacaat
    25921 atgccagctc cactattttt atctgctgta aaagttggaa gcttcgttga agtgaaaatg
    25981 ccagggttaa tggctgcttt gaagcgagta tattagttaa tggaggaaaa tataatgaaa
    26041 agaattttgc attttcaggg acgaatgggt ttaggtggag cagagtcttt catgatgaat
    26101 ttatatcgta aaattgatcg tacaaactat caatttgatt ttttgattta tgaagattat
    26161 gcagatgttc aagactatca ttcagagatt gaaagattag gaggccgtat ttttgttgta
    26221 cctaatccta agaaaaatat ttttaaatat ttaatagaag tgaataagtt actaaaaaaa
    26281 gaatcttttt ctatagttca caatcaggtt tattttggcg ggggaattaa tttatggtta
    26341 gccaaaaaga atggaatcag acagagaatt gctcatagcc atgcaactga agatggaaaa
    26401 tctcaaaata ttgtaatgaa tgtattgcgt aaatttttaa ccaaattgtt gttacaaaat
    26461 gcaacagact accttgccgt tagtcaagaa gctggtgaaa gtctgtttca aaaccatccc
    26521 tttgagatag ttcataatgg tattgattta gagctttata gtaaaaattc agaagctaag
    26581 gttaataaga gaaaagaatt ggatatctca atgagtactt ttgttgtggg aaacattgga
    26641 cgattagaga aacaaaaaaa tcaatcttat ttgttagaga tttttttaga aatactaaaa
    26701 atgaattctg attcttactt gattttagta ggtgaagggt cacttagaaa taaactgaaa
    26761 gccaaagcag tagaattgga tatagagaaa agagtaaaat ttttaggaga aagaaacgat
    26821 attcccgagt tattatctgt gatggatgtt tttgtaatgc cttctcttta tgaaggtctg
    26881 ccaatggttg gattagaagc acaagcagca aaattaaaat tagtcttagc agatactatt
    26941 tccaaagata caaagttaac aaataatgtg gattttgtat cattagatga aacaccacaa
    27001 gaatgggcaa aaaaagtaat aaaagagccg tatgctaata aaataacaca agaactaaag
    27061 agttatgatg tttcgtacac tactgaacaa atggagagta tatatagtag agatcattaa
    27121 aaatgttcaa gatattttag gagtgattat ttttgaaaat atgttatttg atattggcat
    27181 acaatgatat tggcaactta aagagattgg tcaatcgatt gaacgaaaat gcagttttta
    27241 ttattcatat agataaaaaa actgatatag aaccatttat tgaagcattt aaatcatatg
    27301 ataatgttga atttttatgt aataaaaacc gtgttaaaat ttattggggt ggtttttcca
    27361 ttgtacaagc agaaatcaat ttagtaaaga gagcattaca aaatgagaaa tatttaaaat
    27421 atgtattatt atctggtgct gattatccaa tcaaagataa tgagtatatt tataattatt
    27481 ttaaaaagaa tagttcagtg gagtttattc gtggaataga tctagatcaa attaaacata
    27541 aagaatttta ttataaacat atagatgtat atcaaaaaca tgattatcct agaattaata
    27601 ggaataacac aactgcattt aaaattttta gagctataat taatcgttgt ctgaggatga
    27661 ttaaacttcc cccaaaaatt agacaccata aatttgattt gtatcatggt tctcaatggt
    27721 gggctttgtc gaaagaatgc ctaacagaat taatacaaat gtatgaacaa aatcaacaag
    27781 actacctgaa ttttaaaata ggtatgtttg ccccagatga aaaatttttt catacacttt
    27841 tttttaattc ctcgttcaaa aataaaaatg ttattggagg accagattta ccacttgaat
    27901 tgaaaaatat tgaagaaact actttacaaa cttcgaaatt agcaaatatt catattattg
    27961 atccttccat gaacaaatgg tttgatgaaa gggatataga tcaaataaaa gaaagtgaaa
    28021 aattatttgt aagaaaagtt aaatcggatt actctgataa actattaaat agaattgata
    28081 aagaaatttt gaaagttgga aatatagatg aatagaatag tacctgtttt agagaagagt
    28141 ttattaagtc gagatgcgtt tcgagtaata aatttaatca tttgtctgct ttattttatg
    28201 cctagtactt ttaatgtaac taatatcccg atgaaaatag cttttgcttg gggtggaatt
    28261 atagttttgt atgaggtttt tataaagaaa aattttttta aaatgcgttt ttcttggcta
    28321 ctgattggag caatgattag ttttatctta actataatca ttaacagaga aaatttttta
    28381 attccatcta tttataattt tggatatttg ctgattactt tgatagtaat ttttcctgca
    28441 gacttttcac aaagtgaaga ggaaaaaaag aaaaaaatgg tgtattttaa taatgttttc
    28501 attatcataa tttttttagc tgcgctaata tctatctatc aatttatttt tttgatttct
    28561 tatcatgtcc caacaggaac gccaggatta cttgcaagac aaggttttat tgaacatcgg
    28621 ttatttggtg tctatacaag tccaaatgtt ggagctatgt ttggatatac ttctattatt
    28681 ttaagtttaa ttagtgtatt aatttcaaaa ataacaaaaa cgattcgtgt tttttatatg
    28741 atgaatttta tcgtacaaat attttatatt acactttctt cgtctagagg tgcccaggta
    28801 gtaatttgtt cttttttagt attatttctt ttgttgtttg ccaattcaaa atttagaaag
    28861 agcattagag aagtactacc tgtaaaaatc aaatggatgg taagcatatt attagttcta
    28921 ttggtatatt tttcttttgg tactaattat gctaaggatt ttttagcaac aattcctgta
    28981 tcgattgata gaaaagtaga agttatagag tcaaatgaat cagataaaac tccacttcca
    29041 gaaaaagaag taatattaca gcattctagt gataactccg aaatttcagc tggtcgtttt
    29101 acgatttgga aagctgcgct taaagttata agaccaactt tgttatttgg ttatggagag
    29161 accgatttct atgggaatag cgatgcaaaa tttttgaaaa aagtagatct tgatactaca
    29221 gatgtgaatg aactaaaaag agcacatgga aacatgcata atggatttct tcaagtactt
    29281 gtatcaagtg gtataattgc ttttatatgt atctatgctt tctatgtgtt aaatatcttt
    29341 agtttactca aaacattttt tagaagtaaa gtgaattatg cgtttcttgg tattgtttta
    29401 atttttatac tatctatttt tatagatgaa atggtggagg ctcatgtact tttcaataag
    29461 cgcgatgtca tttcaattgt tttctggtat tatttgggaa gcatttctta cttgtattta
    29521 aaaaaggaga attaatatga agacagcttt cattgcttgg actcctttac atttgattaa
    29581 tatattaaat gtttatcaaa atttttaccg agaatcaaaa ggagatttat ttatttattc
    29641 tgaatttgaa aacgctagaa gatatagtga gcgtataaaa gaattagggc tatttaatga
    29701 tgttgttttt gtgaatcacc ggaaaatcgg ttcttccttt aaacgtaaaa ttaacttgtt
    29761 aactaaccat aatcaatttt tctcggatga tttatccgtt tatgatgaaa tttttataca
    29821 gggggaaaat tatttcacaa aaatactttt ctccaatgtt tacaaaaaaa atccagatgt
    29881 aaaactaaat tatatagaag atggtttggg tacttatttg aagaagagta tattttcaac
    29941 aagtaaaaac gttcaaagag tttttaaaat attaaataaa aatagcatct tcaaaaaaaa
    30001 aattgacgat tattttgtct attgtccgga attgttactt tatgatgtaa aaaatataaa
    30061 aaaaattccc aaaatagatg aaaacagtaa attgatgaaa aacatacaat caatctttgg
    30121 gatagaagaa gtttttgtcg gagataaatg tatctttttt gatcaacctt tattatctga
    30181 tggttatggc atagatgaaa cagatatttt cgctaaagta aatagtatat tttctggaaa
    30241 aaaagaaact ttaattaagc ttcatcctag agcaaggaca aatcgttatg gtgaaaaaaa
    30301 catattgaat tccagtttac cgtttgaagt cttgctactt gaaaatgact tctcttcaac
    30361 acttttgatt agtcctatat ctacaatttc tttttcacca tctatgatgt ttgaaaagac
    30421 gatcaactct attatgttgg ctaaattaat tttaaatgaa tatacagcat tatctgaaga
    30481 taaaaaagaa acattgacaa atattgttct tttctgtgaa cattataacc gtctatcaga
    30541 agaaaaaatt ttactcccag aaaactgggc agaattaaga aagttggtag agtatgggat
    30601 tatttaacac actttccaag tcaaaattag ttaagtccag tttctggtat actataggga
    30661 atttttttat taagggaatt tcattcataa ctattcctat ttttgtaaat attactactc
    30721 aagcagaata tggtttgatt aataatttca cttcatttgt atcaattttt tcgattcttg
    30781 ttgggctaag cctaaattcg gcaattaata atgcaagttt tgactataaa gatgacataa
    30841 atgaatttat gtcatcggta ctttctctag ctacactttc gttaattgtg ttcttattaa
    30901 tagggaacat atattttttg ttttcaaatg ctttttttga tatgaaccaa cttgtattta
    30961 atttgatgtt attacaaagt tttgctagtt ttgtaattag ttatatccag tcttatttta
    31021 cgattaactt taaccatttt aaatatttga ttttatcttt tatatctact tttttgaata
    31081 ttgcaatctc tgtagctttg atgcagacat tatattttga tgataaatat gttggcagag
    31141 tagtcggcgg aacactgtca tttgtgttga taggtttagt aatttattta agaataatga
    31201 taaagggtgc tcattttttt gataaaaaac agtggagata cgctttaaaa atctctttgc
    31261 cgctaatccc acattcctta gcaaatatag ttttagcaca attcgataga attatgatta
    31321 attcttattt aggtcctgct gcggggggaa tatattccta cattagtaac ataggtatta
    31381 ttctaagcgt aatatggatg tctactaata acgcctgggt tccatggttc tatggtgaga
    31441 tgagtaaaaa taatgaaaaa aaagtgaaga aagttagtaa ctattattta gtagtcttta
    31501 ctttgattac gatgattgta atggtagttt cgattgattt agcaaaattt atggctccga
    31561 aagactatta ttctgggtta tctttagtgg ttccgataac cttaggctat ttctttcagt
    31621 ttttatatag tttaccagtt aatgctgaat tttatgagaa aaaaacatcg tttattgcga
    31681 ttggtacagt agcaagtgct atagttaata tactattaaa catgattttc atacctaggt
    31741 taggaataat tgctgctgga tacacaaccg tatttacgta ttttttactt tttatttttc
    31801 attataatat cgctaaaaaa attacgaata aacaattatt tgacacaaaa aaaatatgta
    31861 tcataataat ttttgtatca attttttctt tgatacttca tattttaatc aatcaaatgt
    31921 tgattcgtta cgttttgaca tcattattga taattagttt agttatatat tttttaaaga
    31981 ttaagaatcg aattatagat gactataatt cataggagga gatataagtg tttaaaggaa
    32041 aaacaatatt agtaactggc ggaactggtt ccattggaag tgaaattgtt cgtcagcttt
    32101 taaaatatga accaaaagct atcagaattt atagtagagg tgaagaaaaa caattttata
    32161 tgcaacaaga gctctcaaat cacactaatt tgagattttt aattggtgat atcagagact
    32221 ctcaaaggat tgattatgca tgtcgtgatg tggatattat ttttcatgct gccgcaatga
    32281 aacacgttcc agcaagtgaa tataatccta tggaagctgt aaaaacaaat gtgatgggaa
    32341 ctcaaaatgt aatagatgca gcgatcaaaa ataatgtaaa acattttgta ggtatcagta
    32401 cagataaagt agtgagtcct actaacacca tgggtgcgac aaagttattg agtgaaaaat
    32461 taataatagc tgccaatcag tataaaggaa aaacagaaac aatattttct tgtgtaaggt
    32521 ttggaaatgt catggggtca agtggatctg ttattcctct ttttagtaaa caaataaaaa
    32581 acaataaaga tattactttg acagatgttg atatgacacg atttatgatg tcaataccgg
    32641 atgctgcaag attagttctg tctgctgcta tgaattcaaa atttggagaa acatatgtct
    32701 taaaaatgcc ggcaatcaat attttaactt tagctcaatc attaataaaa atatataatg
    32761 atacacatga tgaaaaatat agaggtgaaa taaaagttgt aggtatcagg ccaggagaaa
    32821 aattatatga agagctaatg actacagacg aaattgaacg ctcatatgaa aatgataaaa
    32881 tgtatatcat tccaccacac tatcatgcta ctgacgaact gtacaaagat tatgaaaaaa
    32941 ttaaaatcac tgcatactct tcagatgcaa cagaattaat gagtggacag gaatttatta
    33001 gaatcgcaaa agagaataat gtcgtgactt ggtaaggagt tttatgatga aatcagaagt
    33061 atatgtaatt gcagaaattg gtgtaaacca taatggaagt ctagatttag ctttagaaag
    33121 tattgataaa gctaaagaat gtggagctaa cgcggttaaa tttcaaacat ttaaaacgaa
    33181 taagctagtt tccaaaaagg ctccaatggc aaaatatcag aaggataatc ttaaagagga
    33241 cagttctcaa tttgaaatgc taaaaaaatt agaattatct caatctgatt ttattagaat
    33301 aaaagattat tgtatacaaa ataaaattga ttttttgtct actccttttg atgaagaaag
    33361 tgcagacttt ctaaaacaaa ttggtgtaca aggatttaaa attggctcag gagatttaac
    33421 aaatttacct tttcttagaa aaatagatat gtatgggttg ccagttttat tatctacagg
    33481 tatggctgat attcatgagg tcagtgaggc tgtagattgt ttcatagata gtccagttac
    33541 tattttacat tgtacttcaa attatccggc tagttctgaa gatattaatt tattggcgat
    33601 taacacatta aaagaaatgt tcgggatacc cgttgggtac tccgatcatt cattgggata
    33661 tgatgttggt atttgtgctg ttgcgatggg tgctaaggta attgagaaac attttacctt
    33721 ggataaggaa ctacctggtc cagatcataa agcttcttta gatcctcagg aatttgcaga
    33781 ttttgtaaag catattagaa acactgaaat atttttaggt gatggtgtta agcgagcaat
    33841 gccttctgaa cttagtacaa aagaagtatc aagaaaaagc ctagtggtca ctgagaattt
    33901 aaatgtggga gatattctta cagagaaatc tcttacaata aaacgtcctg gaaatggaat
    33961 agctcctaag tttttatatc aatatattgg caaaaaagtt aaaagaccaa taagtgcaga
    34021 tagtgttctg cttgaggaag atgttgaatg aaaaaaatac ttatagtaag tggaacacga
    34081 gctgattatg gaatatataa acctgttgct gagaatttac tacaagataa ttgggatgta
    34141 tcatttgttg tttctggaat gcatttatcc cataaatatg gatttactaa agaattgatc
    34201 ttagctgacg gatttagaat tagtggagaa gttcaaagtt tgtttttaga gaacaataag
    34261 ggaaacatgg ctcggtctgt ggcattagag ataaatggtt tcacaaatat atttgaacaa
    34321 actagccctg attttgtatt attactaggg gatagaggag aaatgttggc agctgcgata
    34381 gcttgtttgt atcttggaat caaaacagca catattcacg gaggcgaagt gtctggtact
    34441 gtagatgaat caataagaca tgccataacg aaaatagcat ctgttcattt tgctgctacg
    34501 gaaaaaagca aagagcgttt aataaaaatg ggggaagatt cttggagagt ttttcccgtc
    34561 ggagctccta gaatagatac tattttaaat aatagattac ctagttttag agatataaaa
    34621 gaaaaatatc atctaaaatt tgaaaagaaa aaatacgttt tgcaagtatt tcatccagtt
    34681 gtaaccgaac tagaaaatat agaaaaacaa attttagctt tgatcaattc aatggaacaa
    34741 caatctcaac caattatttg cattttacct aatagtgatg ctggtagcga aattatcaga
    34801 aaaatgtacc agaaatattt aaaaacggat attatttata ttacgaattt agagccggag
    34861 gaatacctga ctatattaaa agagtgcaaa tttatgatcg gaaattcttc ttctgggata
    34921 attgaagctg catcattcaa aataccagta ataaatttag gaaccagaca aaatggacga
    34981 gaacaatcta aaaatattat aaatgcaaat ctaaatgaaa atgaaattga aactgctttg
    35041 aataaaatac aagatcctca gtttataaaa agcttgaaaa atgttaagaa catctacggt
    35101 gacggcaaca gttctagaag aataacaaaa acattgaatc aaatattgaa tgatggggaa
    35161 gatattaagt ggattcaaaa acaaatagct tattaaaata tactagtgat ccatacatta
    35221 ttattggggc tggcggacac gcaaaagtca ttattgaaac cttaagatta gaaaataagt
    35281 taatatatgg attttttgat gataagcaaa ataaaatgca agatctgaaa tatcttggga
    35341 aaataaacga attgaatagc tgggcggata gcaaaaaaaa aatctataaa tttattgtag
    35401 ctattggtga ctgtgtagta agaaaaaaga ttataaaaaa attgaatatg ccaagtgagc
    35461 aatatggaat agctgttcat ccaagtgcta tagtggcaaa agaaatttca attggttatg
    35521 gaactgttat ttttgcgaat gctgtaatta acccagatgc tgtaattgga gagcatgcta
    35581 taataaacac aggtagcatt gttgagcatg attgtagaat taataactat gttcatttat
    35641 ctccaggtgt atgcttagcg ggtggagtac atgtaggtgt aggaactcaa ataggaattg
    35701 gttctcaatg tatccagatg aagaaaattg gatcttggtg tatgataggc gcagggagca
    35761 ctattgttaa ggatattccc tcacattcct tagcttatgg caatccagct aaaattaaga
    35821 aagaaggaat taattttgaa ttctagaatt ttattatcaa gtcctcatat gggaggaaca
    35881 gaagaaaaat atgttgctaa ggcttttaaa gataattgga tagcgccatt aggtgaaaat
    35941 gtggataaat ttgaagaaag tattacaaaa tatactggtt ctaagggcgg agcttctgcg
    36001 ttaagttcag gtacagctgc catacatctt gccttaagac tgcttaatgt tggaaaaaac
    36061 gatattgtct ttgctccttc atttactttt atagctacag taaatcctat tttatatcaa
    36121 ggggctgtac cagtgttcat tgactcagaa cctaatacat ggaatatgag ccctgttgca
    36181 ctagagagcg cattagaaga agctgaaaga aacaaccaat tacctaaggc aatcatcgta
    36241 gtgaatttat atggtcaaat ggctgattat gaacgaattt tagctattgc aaaccaattt
    36301 aatgttccag tgatagaaga tgcagcggaa tcgttaggtt ctacttataa gggaatttcg
    36361 tcaggcttat ttgggacaat tgggatctat tcatttaacg gtaataagat catcacaacc
    36421 tcaggtggag gagctctagt gtctagtgat aaaaaatatg ttgagcattc aagattttta
    36481 gcaacacaag caagagatca agcaccgtat tatcaacata gcgaaatagg ttataattac
    36541 cgtctaagca atatttctgc cggtatcgga cgtggacaaa tggaagtatt gaatgataga
    36601 gtagctaaac gaagagaaat tttcaatcga tataaggaag ctttttctgg aattgataga
    36661 attgaaatga tgccagaagt ggatggcatg gtgccaaatc gttggttgtc tactttaacg
    36721 atcaatgatt ataattctat tgatcaagta gttaacgtaa taaataaact aaatgaaaaa
    36781 aacattgaaa caagagcttt gtggaaacca ctacatttgc aaccagtatt tcaaggggta
    36841 aaaatgtatt accatgataa taatcttagt gtgtcagaaa agttatttaa tagcggctta
    36901 tgcttacctt ctggaagtaa tatgacagat agtgaacaag aatttgttat tgatgagttt
    36961 aaaaaaatat tcaagtaggt gtgatatttt atgaacatag gaattattgg tttaggttcc
    37021 atggggaaaa gaagacttag actcattcaa gaacacttta atgatatttt actatgtggt
    37081 gttgatacta atgaggcgag acgaaaggaa gttgaaaagg aatttggaat accaacttat
    37141 aatagtctaa cagaggcaaa aaatttgttt gaaataaatt cagtttttgt ttgtacatcc
    37201 cctataaccc atgaatttat tataaaagag gctttaaatt tagattgtaa tatctttacg
    37261 gaaatcaatt tattaaatga atattatgat gaaataatcg aagtagctaa aaagaaagat
    37321 agattacttt atctatcttc tacatttttg aagagaaaag aaattcaata tattaagtct
    37381 aaagtgacgc ataataaaaa aatttcttat caataccatg taggacagta tttacccgac
    37441 tggcatcctt gggaagatta tcgcaatttt tttgtttcaa acaaaaaaac taatgggtgt
    37501 agagaaatat tcgctattga attaccttgg attatagata catttgggaa agttaaatcg
    37561 tttactgtaa tgacccagaa attatccaat cttgatattg attattcgga cagtttcact
    37621 cttattattg agcatgaaac tggtatagtt ggaactttaa gtgtagatgt tgtttcaaga
    37681 gtagctaaaa gaaatttaac tataattggt gaaaagattt taatagaatg ggaaggtaac
    37741 ccggattctc tattttcatg gaatgaagaa acttctagta tgacagatat tcaattgtac
    37801 gatgaggtta tgcatgataa taattattca catactatta ttgaagatgc atatttagag
    37861 gagattaaag aatttttcac gcttttggaa aagaaaatgg aaccccaata tacttttgaa
    37921 aaagataagt atattattaa cttaattaac gacattgagg taaattaatg atgaaagtaa
    37981 ttttttttgg gttagggtca attggaaaga gacatctaaa aaatctaata agctatggaa
    38041 aaaagaattc tattaatttt gatataacag cttatagtag cagtggaaaa aaagagtttg
    38101 aagaaataac gtatattcaa aatagagacg atttggatac acattatgat atagctttta
    38161 ttacaaatcc gacttttaag catctggaaa cactaaatgt aattgttgaa aaagttgatt
    38221 acatattttt agaaaaacct gctttttctg agagtattga tcttactcaa tataattatg
    38281 attacaaaaa tatttatgtt gctgctccac tgaggtataa aaatatcatg gaagttgttc
    38341 gtaatagtat tcaaagtaaa aaagtatatt ctgtaagagc tatttgttct acttatcttc
    38401 ctgattggag aaaaggtact gattatagaa ataattattc tgcctttaaa tctatggggg
    38461 gaggagtaga attagactgt atacatgaat tggattatat tataaaccta tttggtttcc
    38521 ctaaatcagt aaaaaaacag tttggtaaat attcgaatct tgaaattaac agtaatgatt
    38581 tagcaacata tatattgaga tacgatgata aaattataga aattcattta gattattttg
    38641 gaagagttcc tacaagaaaa tttcaattgt ttactttaga tgaatgtatc aatgttgact
    38701 tattaaataa taaaataagt tttgaagaaa acattgttaa agaaatgcct gaatctgcta
    38761 atgatatgta tgagaaggag ttgaaatatt tctttgaaaa cgtagtaaca ggcaaagaaa
    38821 actggaatga tttatatcat gcaaacgaag tattgaaaat agctgaggag aaataaatgg
    38881 aaattttatt tactatttgt ggtagagctg ggtctaaggg gtttaaaaat aaaaatttga
    38941 aatatttggc aggaaagccc ctagtccatt tcgctttagc aatgatagat atctatcaac
    39001 aaaaacatcc tgaacataat tgcacgactt gtttgaatac ggacagtaat gagttagtag
    39061 aattgacgac taagttaaat ccgtctgtga aaattgtaga acgtaaacct gaattagcta
    39121 cagatactgt agggaaaatt gaagtgatac gtgattcatt cttacaacta gaaagagaag
    39181 ataatatatt tgatttaatt atagatttag atttgacttc acctttacgt gaagtttcta
    39241 atcttgaaga attaatagat gaacatctaa aaaatttaga taaagacgtc tacttttcag
    39301 ttgtttctgc aagacgtaat ccctatttta atatggtaaa aagaattgaa aatgaagtta
    39361 ctatagttaa taaatcaaga tacacatcta ggcaacagac acctgaagtt tttgaaatga
    39421 atgcctcaat gtattctttt aaacctgaat ttctaaaaaa aagtaattat atttttgatg
    39481 ggcaatgtgg gataataaaa atgagagatt atctaatttt agatattgat agtgaagaag
    39541 attttaaatg gttagaattt ttgtttccta aatttttaga agattcttcg gagcttcaag
    39601 atatctataa ttatttatat cggaggtaaa aggatgagct gttatcttat caccggtggg
    39661 gctggcttca ttggttctac cttagccaat catttaggag aagaacatgt ggtaatcgtc
    39721 gtcgatgact tatcaatggg aaaagtagaa aatcttgata tgacgaaaaa tatcactttt
    39781 attgagggag atgtcgctga gaaatccttg atggaagaga tcatgaaagc taataaattt
    39841 gattatatct ttcatttagc agcagttgct agtgtggcag attcagttgc ccgaccagta
    39901 gaaacacatc gtgtgaattt tgaaagtgta ttaatgattt tagaattgat ccgcaagtat
    39961 caacctgatt taaaacggat cgtgtttagt tcttcagcgg ctgtttatgg ggatgaaccg
    40021 actttaccaa aaaaagaaga gtccgtgatt cgcccattga cgccttatgc ggttgataaa
    40081 ttcgctgcag aacaatatgt gctagattat tgtcatttat atgatgtacc cggtagcgca
    40141 gttcgttttt tcaatgtcta tggtccaaat caaaatccga attctccata cagtggtgtg
    40201 atttctattc tcgtagatcg ctataagaaa caaatcgtag gagaagcaac ttcatttaca
    40261 ttatttggtg atggaagcca gtctagagac tttgtttata tagatgatgt aatccaagca
    40321 ttacttttag ttgcgaatga ggaagctgca ctggggaaac aattcaatgt tggaacgggt
    40381 aaagcaacca cacttttgga attgattcat gcaatcaatg aaattctcgg tgtagaattg
    40441 acacttgaat acaaagaaga gcgttcgggt gatatccatg actctctagc agatattact
    40501 aagatccagt caattggcta tcaaccgaaa tatggtattt taagtggaat gactaattat
    40561 ttgaaaactg aaatcaatta aaacatacta agataagatt agagggtaaa ttcaggctct
    40621 ttgtcaataa ggactgatga gttttgtaaa atcaaatctg ggatagaa
//
LOCUS       DACDXE010000020        38856 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.020, whole genome shotgun sequence.
ACCESSION   DACDXE010000020 DACDXE010000000
VERSION     DACDXE010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.020"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..204
                     /locus_tag="IWM23_001047"
     CDS             <1..204
                     /locus_tag="IWM23_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9883707.1"
                     /translation="SGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDGSVRMIKAQEEP
                     DIDSLVDSIMNQYEDALKDLVDL"
     gene            201..599
                     /locus_tag="IWM23_001048"
     CDS             201..599
                     /locus_tag="IWM23_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9883708.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(1266..2210)
                     /locus_tag="IWM23_001049"
     CDS             complement(1266..2210)
                     /locus_tag="IWM23_001049"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9883709.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(2506..3450)
                     /locus_tag="IWM23_001050"
     CDS             complement(2506..3450)
                     /locus_tag="IWM23_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883710.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            3671..4885
                     /locus_tag="IWM23_001051"
     CDS             3671..4885
                     /locus_tag="IWM23_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9883711.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4907..6922
                     /locus_tag="IWM23_001052"
     CDS             4907..6922
                     /locus_tag="IWM23_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HAP9883712.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(7005..8378)
                     /locus_tag="IWM23_001053"
     CDS             complement(7005..8378)
                     /locus_tag="IWM23_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9883713.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(8404..8688)
                     /locus_tag="IWM23_001054"
     CDS             complement(8404..8688)
                     /locus_tag="IWM23_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883714.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(8690..10705)
                     /locus_tag="IWM23_001055"
     CDS             complement(8690..10705)
                     /locus_tag="IWM23_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9883715.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10884..11189)
                     /locus_tag="IWM23_001056"
     CDS             complement(10884..11189)
                     /locus_tag="IWM23_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAP9883716.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(11278..12309)
                     /locus_tag="IWM23_001057"
     CDS             complement(11278..12309)
                     /locus_tag="IWM23_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAP9883717.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            12516..13391
                     /locus_tag="IWM23_001058"
     CDS             12516..13391
                     /locus_tag="IWM23_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9883718.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(13543..14385)
                     /locus_tag="IWM23_001059"
     CDS             complement(13543..14385)
                     /locus_tag="IWM23_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9883719.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(14477..16354)
                     /locus_tag="IWM23_001060"
     CDS             complement(14477..16354)
                     /locus_tag="IWM23_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9883720.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(16351..17772)
                     /locus_tag="IWM23_001061"
     CDS             complement(16351..17772)
                     /locus_tag="IWM23_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9883721.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(17775..21164)
                     /locus_tag="IWM23_001062"
     CDS             complement(17775..21164)
                     /locus_tag="IWM23_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9883722.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            21421..22812
                     /locus_tag="IWM23_001063"
     CDS             21421..22812
                     /locus_tag="IWM23_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9883723.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22885..23532)
                     /locus_tag="IWM23_001064"
     CDS             complement(22885..23532)
                     /locus_tag="IWM23_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9883724.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(23540..24226)
                     /locus_tag="IWM23_001065"
     CDS             complement(23540..24226)
                     /locus_tag="IWM23_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883725.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(24232..25305)
                     /locus_tag="IWM23_001066"
     CDS             complement(24232..25305)
                     /locus_tag="IWM23_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9883726.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            25527..26201
                     /locus_tag="IWM23_001067"
     CDS             25527..26201
                     /locus_tag="IWM23_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9883727.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            26206..27546
                     /locus_tag="IWM23_001068"
     CDS             26206..27546
                     /locus_tag="IWM23_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9883728.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(27695..28681)
                     /locus_tag="IWM23_001069"
     CDS             complement(27695..28681)
                     /locus_tag="IWM23_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAP9883729.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            29405..30436
                     /locus_tag="IWM23_001070"
     CDS             29405..30436
                     /locus_tag="IWM23_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9883730.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            30545..32077
                     /locus_tag="IWM23_001071"
     CDS             30545..32077
                     /locus_tag="IWM23_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAP9883731.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            32216..33028
                     /locus_tag="IWM23_001072"
     CDS             32216..33028
                     /locus_tag="IWM23_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAP9883732.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            33018..33503
                     /locus_tag="IWM23_001073"
     CDS             33018..33503
                     /locus_tag="IWM23_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9883733.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            33526..34071
                     /locus_tag="IWM23_001074"
     CDS             33526..34071
                     /locus_tag="IWM23_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAP9883734.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(34144..35787)
                     /locus_tag="IWM23_001075"
     CDS             complement(34144..35787)
                     /locus_tag="IWM23_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAP9883735.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(35916..38144)
                     /locus_tag="IWM23_001076"
     CDS             complement(35916..38144)
                     /locus_tag="IWM23_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9883736.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(38177..38656)
                     /locus_tag="IWM23_001077"
     CDS             complement(38177..38656)
                     /locus_tag="IWM23_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883737.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11389 a         7319 c         7057 g        13091 t
ORIGIN      
        1 tctggcaata gcgatgtaac aactatccca ccagaagtaa aagaattcat gggtgtgcag
       61 acaggtgacg caatcagtta tgtttttcaa tctgatggtt ctgtacgaat gatcaaagca
      121 caagaagaac ctgatattga ttcattagtt gattcgatca tgaatcaata tgaagacgcg
      181 ttaaaagatt tggttgattt atgaggtatc tttcgactaa agaaattatc aaaatcaacg
      241 cgaaagttat tttacgtcac tcgccaggcg aaatgatcgg tgtaaaagat gcaaatgcat
      301 tagatatggc cgtcaaacag ccttcacaag cagtattcaa tcaagaactc tatcctgaaa
      361 tctatgaaaa agcggcgatt ttagcaatta atttagctaa aaaacatcct tttcataatg
      421 gcaataaacg aactgctctt gttgccatgt tgttgtttct tcaattgaat aattgtccag
      481 ttgctttttc tcgtcaagaa gctgttgatt ttatcataat gattaccact agttcattgg
      541 atttcgattc actaaagtcc aagataacca gttatttacg acaaaccgca ataaaatgaa
      601 cgaattaaaa actttatttc tcaattagat cagcatgtca ttaaggaata tgctgatttt
      661 tttatgttta atcatacaaa atagtttttt cataaaaata tggaaaatat ggattaattt
      721 tgaaataatt gatttaaaag gaatacaaaa gagtgaatag taggaaatag tggtttagaa
      781 ctagtgggct gattttttat atattttagt ttacataata tatattatac aaagtagatt
      841 taaaaaatga taaacctctc tcgaacggtt ttttatcaaa aatcgtcatt tttccatcaa
      901 aatctgctta tgcaaaatta taaaaaaatt ttgcagtttt tcttactttt tttgtcgttt
      961 aattgaatga taaaaaccag tttctttatt cagttaatca aatatgaacg aattagatat
     1021 gaatcattgt atttgccgtt gcggaaggat tttttaatta accacagata aacattgata
     1081 acgattcgct tattgtaatc aacagattaa atttcatatg ttcatatgta cggatacatt
     1141 ttcggaccga ttaattttaa ccattctgtt tatttgctta cctattagat atcaataaaa
     1201 aactgcttaa cagctatttc agctgttaag cagttttagg atttttagtt catattcatt
     1261 gtttcttata atacagtgtt caatacttct cttaaagcac ttgttgattt gctgaacatt
     1321 gctttttctt cttcgttgat tgataattca acaacttcac gaacaccatt ttcgtcaacg
     1381 attgatggca cacctgtaaa gatatctttt tcgtcgtatt caccagttaa ataagcagat
     1441 acaggcaata cagcttgttc gttattcaag atagctttta cgatacgtgc agtactcata
     1501 ccaataccat agtaagttgc ttttttacga tcaatgatct cataagcagc attacggact
     1561 ttatcagcaa tcacatctaa ttcatcttgg gcaatacgat ggtctttttc tacaatttca
     1621 aacactggtt taccaccgac agttgtatgt gaccatgcag caacttctga atcaccatgt
     1681 tcacctaaga tatagccgtg gacgctgcgt gggtcaactt ttaatttcaa tgcgatttct
     1741 ttacggaaac gagttgtatc caatgttgtt ccagtaccga taacacgtga tgttggcaga
     1801 ccagattcgt tccaagcgat atatgtcaaa atatctactg ggtttgaagc aacaacgata
     1861 ataccgtcga atcctgatcc catgatttct tttacgattt ggcgcatgat tgacgcattt
     1921 gttttaacta aatctaaacg agattgacca ggtttttggt tgataccagc tgtgataaca
     1981 acgatatcag catctttaca ttcttcatag ccacctgacc atacagccac attttcatcg
     2041 ccccaagcca taccatctaa aaggtccaat gcttcccctt ctgctttttc ttgattcaca
     2101 tcgatcaata ctaattcatt agagattcct tggttgatca ttgcataagc gatacttgtt
     2161 ccaacaaaac ctgtaccgac gattactact ttacgacttg tttttttcat gaataaacag
     2221 ctccttttca taactatttg ttactttcat gaaaagtata gtcttgttta ttcattttat
     2281 caacatgtta attataatag ttgttattat aatttttgat ggaaacgaat tcctgaatac
     2341 gcataacaga taaatgacaa atgtttacac ctatatcccc tgataataca accaaaagtt
     2401 tagaactgaa aataaatcca aaaaaattcg ctgcaacgat ataataagac agcaatcgca
     2461 cctaaaacaa ttttccttca tatgctttag tgaaagattt tcttcttaat gtttatttct
     2521 ccattcattg ggagtacatg ttgaatactt cttgaataat ttgaataaag aagtcgtttg
     2581 tttatatccg atacgattag cgatttcttc caacgaaaaa ctcgtttctt gaatcagttc
     2641 caacgcaaag tcgaattttt tctttagaac cagttcttga aaggaagcat caaaatacat
     2701 tttcaattta ttactaagat agaccggatt gtagccaaaa tgagctgctg tttcttttaa
     2761 cgtacaagaa gtaaaatgct tatcgatata atctaatact ctaagcatct cattttctgc
     2821 gcatccatct gctgatgaaa agctttgttg ttctaataaa tgagtcaatt cgatcagtaa
     2881 tagtgatgta tagagattta acgaacggat cttatgactg tatgtatcaa aataaatcaa
     2941 cagcatattt tcaatcaaat gcgtcaaacg acttgttttt ccgccgcgaa aatgaatgaa
     3001 ttgtgtatgt tccccttttt cttttgatgc atttatcaaa aaagaagaaa gtagattatt
     3061 tgtgcttgcc aaatgattca atatctctgt agataaggtt tcttctttta ataagatgtt
     3121 gattaaaata tcctcttgcc ctgtgtattc gattctttga gtagcttctt tatcaatgat
     3181 caccacgtca ctttcttcca tctctatcct ttctccatta ataatttgta aacatgtacc
     3241 gctatacata taattgattt caacaaaatc atgtgtatgc tcgggaactt ccgcatatcg
     3301 acggtgttta ctgatatgaa tatttccctg ttgaaataga tcccctcggt ggatttttgg
     3361 cggtgtctga agatggtaag ctaagtttaa atcattgata ttcattcctt gttcacgctg
     3421 cagagtttcg attgattctt cgttacgcaa aaaaaatgtc taattcagct agttccaatt
     3481 gactccctcc tttttgtaag cggtttcttt cgcttattag ttcacgctgt actgctatta
     3541 tatgcaaagc tatttttggt ttcataaaga ctttctatgg ttgggacatg aacttcctgt
     3601 gattccgcta ttgagagaat aaccacttca tactatattt ttgataagcg cttacaaaaa
     3661 tgaaaggaac atgcttatga ataaaaagat gatgaaattt tctctgctgt cagtctctct
     3721 tattttgact tctgctggtg cagtcacgtc tgcaattccc agtatgtcag ctacttatgc
     3781 cacgcgttct ttatctgctg ttgaattatt aacgactgct ccctctttta tggtcactat
     3841 ttttgttttg attagtagtt ttattgctag gaaaatcgga caaaaacaaa ctgtgatcat
     3901 cggattagtg attgcatcga tttgtggtat cctccccctc ttctcacaaa gttacagtat
     3961 tgtcttagct tcaagagctg gactaggtat tggctttgga ttgatcaatt ctttggcagt
     4021 cagcatgatt gctgcctttt atcacggaga tgaacgagcc tctatgattg gttttcaaag
     4081 tgcatttcaa ggatttggtg cagctttaat gacatttgtt gcaggacagc tagtacagtt
     4141 cggttggcaa tatacctttt tagtctacgc catcagctta cctattcttg tcttgtttgt
     4201 tttgtttgtt cctacacctg aaacaactga acttcaatcg catggcacag ccccaacaag
     4261 acaagtaatc aaaacgaatc ctaaaatgat tagttatggt atttttctct tgctggtcat
     4321 cgtggtatat aatgctgtat cgattaaatt agccacagtc ctgacgactt attctctagg
     4381 aaacgaaact aatgcagcga atatcttaac aatcatgcaa ttggcaagta tggctgccgg
     4441 agtttgtttt ggctggcttc agacaaaagc acatcgctat atgcttacac tttctttact
     4501 actgatggga atcggctttg tattgattgc cactgtgata aacctgtatg tgattgggtt
     4561 tggtgcgatc ctaacaggaa tttctttttc gttattcatt ccttacttat tcaaccaagt
     4621 aagtatccaa gcgcccaagt acgcggagag tttcaatact tcaatcttat tggttggcgc
     4681 taatttaggt actttcattg caccatatgg tttgctcttt ctctcttttg cttctcttgg
     4741 gagtaaaaca cttgctgttt tcgtcaatgg cagtatcttg ctgttgatta gcgcgcttat
     4801 tagccttcta gtagtacaca ggacgaatag aaagacagtg ataagagtag atgaagaaat
     4861 aaaagaacaa ttgacaacaa aataaatttg atggaggtcg tgtaaaatga caaatgaaaa
     4921 acataaagat agatggcttt ggtatcctgg agactttgag atccgccatg gactgctaca
     4981 aaatttccaa cgggaagaac gaggttttga ctggccagcg tactggtaca tggatgattg
     5041 ccatcggaac gtcaagttta agagatatta ctttcttgat caaccttcca tgtttaaagt
     5101 gacgatccaa ggagttgggt atgtagaaat taatggacaa aaacatccat gtggaaaatg
     5161 gctgacttgt ccagctggta aagcaaaaat tcgtatcttt gtaggacata catcagggct
     5221 accagcgatg tttatcgagg gagacgaagt aaaatcagat attggctgga cagcaagtaa
     5281 ttttattgaa gaatatccag ctggctggag tccgctttat gtcgatatcg caaaagatcc
     5341 aaatcaaatc tattatcaaa aagaatgcgt ccatccagtc actgaaacgg agattggcgg
     5401 aggaatctta ttcgattttg ggcgagcagt gaatggctgt gtagaactaa tatctctgga
     5461 tgaccatatc gaatctatca ctctatgtta tggagagtcc gatgtagaag ctatggatat
     5521 cgattattgc tactataagc aaataggaat gacaagaaaa atgaaagctc gtaaacgcgc
     5581 ttttcgctat ttgtttatac cagaggtttc tactggaatg gttcaacttc aagtttttca
     5641 tgaattttta tctctttcga aacaagctgc tttttcaagc gattcttctt tactgaatca
     5701 gatctggtca gtatcagaag aagccttcaa gttatgtagt gatttgtttt ttatcgacgg
     5761 tatcaaacga gatcgctgga tttggtctgg agatgcgtat caatcattca tgatcaatca
     5821 atatcttttt tttgacgagg aaatcaataa acgaacgata ttagcattaa gaggacaaaa
     5881 tgaaatcaag cagcatatga atactatcgt tgactattcg atcttgtggt tgattggtat
     5941 cgaaaaccac tatatgatga cgaaagacta tgaattttta cgcatcgtct accctaagat
     6001 gcaatcatta atgagatact tgatggagca aacgaatgaa ttagggttta tttatgggag
     6061 agcaaaagat tggatattta ttgattggtc tgaaatggat aaagaaggaa cgcttgctgc
     6121 agaacaagtc ttattactga aagcctatca ttccatgatc acttgcggag atatcttagc
     6181 tgaaacatat ggagagaacg aaaattatca tactcatctc tatcaagaaa aatatcaact
     6241 attgaaacaa aacctatttg actacttttg ggatgaagaa cttggcgctt ttatcgattg
     6301 ctatgaatct gggagaagaa atgtgacgcg tcatgcgaat attttcgctg ttttattcga
     6361 ttttgctaac aaagaccaac agcaatcgat cttaaagaat gtcttattga atgacactat
     6421 cacacagatt actactccat atttcaaatt ctatgaacaa gatgctttat gcaaactcgg
     6481 acagacgaat atcgtctacc aaacgattct tgattattgg ggagggatgt tatctcatga
     6541 tgcggtaact ttctgggaag aatacgaccc tactcaaaca ggaaatgaaa agtacagtat
     6601 gtatggagat ccttatggaa aaagcttatg ccatgcatgg ggagcaagcc caatctatct
     6661 tttaggacgc tattttatcg gtttgtatcc aacaaagcca ggttatgaag aatttatgat
     6721 acaacctaat ctagattcat ttgaaacact gaattgtacg ctacctatca aagatggaaa
     6781 gatttcgctt gaaaaaacaa aagataagat cgtgattact tctgatagaa caggcggaac
     6841 agtgaaaatg aatggtaaaa gttatcctct tctagcaaaa cagccacttg tcttaacaag
     6901 ctattcaaaa ataaatgcat aaaacaaaaa actgcttgcg acatatcaga agactttaaa
     6961 tcttcttctg atattgtcca agcaattttt attttgaagt ttgcttaatt ctgttcttta
     7021 gaaacaatgt ttgttctttt tccggcaaaa agaaagctag ataatacgac aagtaaaaag
     7081 aaagcgatga ttccaactgt caaggctatt tgaccgtatt ctcctagttt cccaagtaca
     7141 agtcctggta ctagataatc agtatcggca aatgtagaag aagcgatttt aagatctcct
     7201 aatactggaa ggataaataa tggcagccaa ctaatcaata agctattgaa agcagaacct
     7261 aataccgccc ctcttctacc gccagtcgag ttgccgaaga caccagaagc agctccgcag
     7321 aagaaatgtc ctactacccc aggaatgata actgtagttt gcaagcctat catagcaaac
     7381 atggatagtg ttcctacaaa gaagctaaca aaaaagccaa ttaatacagc attaggtgca
     7441 tatggaaaaa cgataggaac atctaatgct ggtttagaat cgggtacgag ttttttagca
     7501 atcccttgga atgctggaac gatttctgcc aacaccattc gaaccccttg aaggacgata
     7561 acaaacccag ctgtgaatgt tccagcttgg atcagtgcaa agattacagc gttgtctcca
     7621 ttgcttagtt ctctttcaac aaatccagga ccagctaaca aagcaagtgc gacatatacg
     7681 atcgacatca agagcatgat cgatacagta gaatctcgca aaaagctgaa acttttagga
     7741 atctttatgt cctctgtaga acggtctttg tttccaaaaa actttccgat ccatccacta
     7801 gaagcataac cgatatttcc ggtatgccct aacgcaactt gattgtttcc agtgatgttc
     7861 ttcataaacg gttgacaaag agcaggcgta atagttaata gtgttccttc gaagaccccg
     7921 cctaataaga tcatccacaa atttgcagta aaccccgcgc ttactaaaat gactgctgtt
     7981 agacaagaaa cataaagcat tgcttgacct gtaagaaaaa tgaatttata tcttgtgaat
     8041 ctagcaagta aaatattcac gatcatccct actagcatga tcaaagcagt aggtgtaccg
     8101 tattgttgca aagcaatcgc cactactgct tcattgctag gaactactcc ttgagtatgc
     8161 agagctgttt ggaacatcga agcaaaaggg gttagtgaat tactgattaa accagcaccg
     8221 ccgatcagta ctaggaatcc gacaaatgtt ttgatagttc cttggatcac ttctgaaaat
     8281 ttcttcttct gaagaacgag accaagcagt gcaatcaaag aaacgaggat ggagggctga
     8341 ctgacgattg acacaaacaa attcaacaat ttatccattg atagttcctc ccttgaatca
     8401 tttttattct tttgacaaga tcttcgttac tttttctttt acttcttcgc tgtcaatgat
     8461 cgattgcaaa ggtacgagct tttcatcagg tatagaagtt agcgtatctt tcaaagaatg
     8521 ctcaatgaag aaataatcag ccatatcgct agatacactt ccaacatccg tatgacttac
     8581 ttcaaactcg tccacatctg ctccaagatc tgcaagtgct tgtttgatgt tcatctctac
     8641 cataaaactt gttcctaatc cactttgaca tactgcaaaa attttcatat cattcgtctc
     8701 cttttcttat gatcgctaat atttcttctt ttgttgatgc gtgtaataaa tcttgaagat
     8761 tttgtttatt agaaagtagc tttgttaaat ttgccaaagc ccgaagatgt gcctcattat
     8821 cgatagcagc taaacagatg aacaaactaa tgggatgggc tggattatca agcagatcca
     8881 caggttgttg aagtttcagc aaagacatcc ctacttcgtt tacgccatct tctggacgag
     8941 catgaggtaa tgcgatgtga tcgccaatat ggataaacgc accatactgc tccacgcgct
     9001 cgatcatagc tttgatataa ctttcttcta ttttattttc tgataatagc ggctgtgcgg
     9061 caaatgtgat tgcttcttgc cagttttttg gttgatccga taattggatc atttcttctg
     9121 ttaataattc agatagcata ggacgttgat cctccttctt ttctaaaagt ttgttcattt
     9181 tacgatttaa tacttgatag agcttttctt cgtctacgcc ttcttttaat tcgatataag
     9241 gcagtagcgc cgacaacaat tcttgggcag aaggcattga aaatcctggt atcagccaat
     9301 ctctttgtac ttgattgatc agctgatttt tttccaagtc agtcatgatt ggatgcagaa
     9361 catagagaga ctttttctta tcagtaagtg tgagaggtac agtagaaaaa ataatatcgt
     9421 aagaagtttc gtcaacctcg tgtaagcggt cagctgaatt agtctctgta aaaaagatca
     9481 tcgggaataa ttgctgaagt tcacttttta agatcaatga agagctaatg ccacttggac
     9541 agacgatcac ggctcttatt tttctatttc tttcttctgc gtccgctttt ctgatctctc
     9601 cgccaaatag tatagtgaaa aaaccaatct cttcttcagg gatgctcttc cctattcgtc
     9661 tttctaacgg aagtaatgct tttcttgtca tttcgtagat ggaagcatat tgatgcttaa
     9721 tttgatcgat catcacatta ggtaaataaa aagaatagga aatacgaaaa taggcaggga
     9781 ccaagtgata aaagacattc atcaataatt cacgaaacga agcaaattct acggccatca
     9841 ggaattccat gcgatggatg acttctgaag aacattccag caaaaaatca agtgcaggct
     9901 cctggacatc tccttcaacg atcgatgcta agcaaagaga gaaaaatata atctccgaat
     9961 tttgatgttc ggaatagcca aggccagtca atacttgtcg actaatgtca gtgactgcaa
    10021 gagaagccaa cagttcagca tcttttacca gttccatgga tgtttttcca attcgaatgg
    10081 ttgtcaaact caaataataa gctaattcat ccattcgatt tggtgcgaat cgaagatctg
    10141 atagtgaaat cgttttatct agcaaatctc taactttggc gtaataatca atggaaaggg
    10201 ttgcggttaa atacgtgagc ccaaatcttc cagcaagtga ctgcatcaaa cgagcagtgc
    10261 gattttttgc ttctcttctt aatattgctt ctgaccctag cagcctaaaa ccagttttcc
    10321 tagtatacga cagctgacaa tcttccactt ctaattcatt tcttatcttt ttgatatctg
    10381 acaggactgt ccctttagaa acctgaagaa aatcttgaaa atgaaaaaca gaaacagacg
    10441 ttgcagtcga ataaaccatc aaataaagaa gatctcttcg ctcttgttcg gtatacacag
    10501 cttcaaatcg agtaatatcc aaaaattttt ctgcccagtc gattgctcgg aatttcttta
    10561 tacggatacg ggtatcatcc acatattcgg caaaaggagt taattgtttg atcgcctcgc
    10621 ttgtatttac ttcgatgatc tctgctgctt tttctagact gatttctctt ggtgttttca
    10681 ggagataact aattttgtag ttcacagcgc tgttcacctc ctacttatat tctagtatgg
    10741 tagcgctttt aaatcgaggg gtgtttggta caacttacag ttggattttt tttacgactg
    10801 taccgaacga tttctatcga atggatacag aaaaaaagca ggtgaccaaa tgtctcctgc
    10861 ttttatgaat tgatttcgtc cgatcaatga tgatcatgtt tattttttga ggtcagttct
    10921 tcaaattttt ctttgacatc ttcaaccgct ttttcgatat ctttttcaaa gtgattgggc
    10981 tcattaggct ttgtttcctc ttctacagaa tttgaaccag ataaagctgt ttctttattt
    11041 tctgtttcac cagagtttgt gccagctaat gattcttctt tgtcttctgt ttcaccagaa
    11101 ttagttcctg ctaaagccac ttcttcttct gttttagggg ccattgaacc tcttaatcgt
    11161 ttgtgttttt caccgtcata atttgtcatg ttggttcctc ctagtagttc tatacttttt
    11221 catcgtacaa taagacagtt tatgcgtcaa aaaacaaacc cgattcttac aaatatgtca
    11281 tatttttcct tctgattctt gaatgctttt gattccttct tccaaaattt tcatttgtaa
    11341 gattgtttct tcatctttga tgactttttc tgcgccatta actaatgtct cgtaaatccc
    11401 ttcgtagaca cggctgtaat caccaacctc ggaaattact ttttcttcat gataactccc
    11461 ttgttcatct atataggtca atacaccata atgttcgggc aaatcgatac caaagtcttc
    11521 atgatcaggc atgtaaaaca ttttcagatg ttcttcttgt cgatcttttg tttgtttcac
    11581 gaacatccct ttttttccgt aaacgacgaa acttggacgt tctttgatgc ggaaataact
    11641 ggattttatc gaaattttca tatttccata gaaaaaatcc agatcgaagt aatcattcat
    11701 tcgtcctttc cctaaaagtt gacgtacatc atagctgata tcatctggtt cgccgaaata
    11761 agaaagaact tgatctactg tatgacaagc atgtccatat aaatagctgt tcgcagtaga
    11821 aaattcatgc gtattttctg gtacttccgg tctgaaataa tcataatgca tttctacttc
    11881 taaaatatcc cctaattttc cgctttcgat tactttttgg actgtcaaga agtcagaatc
    11941 gaaccgtctg ttttggtaac attggataaa tagacctttt tcttttgcta atgcaaataa
    12001 ctcttctgct tcctttgacg tttctgcaaa aggtttctct actagtacat tcttaccatg
    12061 ttcgagtgcc atttttccat attcataatg aaattgacta ggtgtagtga tgacaactaa
    12121 ttggatctca ggatcatccc aaagatcaga cagctgatca gtatattcga tgtcttcgaa
    12181 tgttttccac actggttcag atggggaaaa aatttttttg atattgattt tgttcgtaag
    12241 ttttcttgaa aaaggcaaat gatagcgatt tgtactcttt ccattgccaa gataagcgat
    12301 tgtaagcatg agtggtgcct ccttttattg atgttttcag tatatcctag aaaagcgcag
    12361 aaagtattta taatagctat tctggtaaaa aagttcagaa aaacacttgt tatcattcgg
    12421 tttggtgaaa ttaatgttca aagtaaggat aaaaatcaaa aagatagtag cggaaccaag
    12481 tgtttatcag taagatagaa tttggaggtc agatcatgct tattgaagaa aaactaacaa
    12541 aacaagagtt gtttaccacg acagagaaac ggattgtcga ttacatccgt aggaatatcg
    12601 aagctgcagt ctacatgacg attgaagaat tagcaaaagc tacgtatact tcccattcag
    12661 ctatcattcg tttatgcaaa aaaatgggat tcagcggatt taaagaattc cgtttcgagc
    12721 tggggcggga agttcatcaa ttgattgctc agttcaatca aacgattgat cctaattttc
    12781 ctttttcaag tgaagatagc actcagcaaa ttgcaaagaa aatggcggat ttatcgatcc
    12841 agtcaataaa aaaagcacaa ttgcagattg aacatgaaga tttagatcaa atcggtaaag
    12901 tattgaacaa ggcacagcga atctttcttt ttgcaaaagg tgattctcaa atcacggcaa
    12961 ggaaattcca gaataaaatg gtcaagttga ataagttttt gatcatggct gaagaataca
    13021 gtgattcttc ttggaatgca gccaatttga ctagcgagga ttgcgccatt tttattagct
    13081 acagcggtcg catccatcat tatgaacgaa taatgaccta tttgaagcat gtagaggccc
    13141 ctaccttgct gattaccgga aatcagcatt cagaaatggc caaacaggct gatatgtgtc
    13201 ttgttatatc gcaggaggaa tatgattttg ctaaagtagc gactttctcc tctcaaattg
    13261 ccttcgatta cgtattaaat acgttattct cagtgatata ttctcaaaat tttgaagaga
    13321 atatccgcaa cttgaaaaac aaacaagaat tgatgcaaaa aggccttctt aaagaatatc
    13381 ggaaaactta agaaaagatc ctcatttagt aataagaaag atagtgtttg ataggctctc
    13441 tttgatatca tcataaaaac aggctgaagt tttctttttc aaacctcagc ctgtttgttt
    13501 acggaacgat tcgaatagat atattataga aatgaggaat agctaacttc tgtttcttat
    13561 tttgaacgat tgacgaaaaa gatatataag aaggaggaaa atcagtatac atgcagctaa
    13621 caatccatac aggaaaaatc gtttgttctc ggatactttc tgtacttctt tttgaacttc
    13681 ttttgcatga taaggaaccc ggtgccctct gacaagtaag cgatgactgt tgaccatata
    13741 aggtgtacaa gttaggagtg tcacgaaatc ctgccctttt tcgattctca gatcttttgt
    13801 atcagtcggt tctaccgtct tgatctgatc gacttgataa gcgagtgtcc gtccattgat
    13861 ttcgatatag aaagaatcac ctaaagtcag tttcggtaag tctgtgaaca actttgcttg
    13921 aggcagtcct cgatggctgg aaataacggc atgtgtattt tctccccccg taggataaga
    13981 cgttccttcc aataatgagg cacctttttc tagcagcaca gtattcgttt catcaaaaat
    14041 tggcaaatgg acattgatct cagggatcgt caaaataccg atcgtatgtt tctcaaaata
    14101 ggttctatcc tttttcgcag gttctttttt cttagtaaag ggatctgcac ctggatttcc
    14161 accttctttt gctaattgct ggtttttctt tgtcatgttt tcttggattt tagccatgac
    14221 ttcttcattt tctttgaccg cttgttgctg ataatgagag atgatctgct gatccagata
    14281 attattcagc gcgtcgctaa caaatggata agctagtatc cctattcctg agagaagcaa
    14341 gaaaagcatc agcagatcta gaagtttcct tttctttggt ttcatttgtg ttctccttgt
    14401 gatgactgtt tgataataaa agaaaaagga gcatttgcgt aatccgctgc tcctttttcc
    14461 aattcttgca aaaagactaa atctgactgt gcttgcgtct agcaaagtac agtccagcaa
    14521 tcagtagaag gactactcct gcaccgatat acacatagat tcccttaccg cctgttgaag
    14581 gaagtgtacc tttgtgttta tttggtattt tttcaggaga aatcaactgt cctgctgtta
    14641 cataagattg ttcatcgata gtaaatgcta cacggtttgt taatggaaca tatttttctg
    14701 gcgctttcgt ttcttccaga tagtacgtgc catattttag acctgtcaca tcgattaaac
    14761 cgttacttgt ggttgtaaaa accgttgctg attctttcgc cgatacccag ctgacggctt
    14821 ttgtggatgg gtcgatcgat aaatatttcg ctgtgtcact atcttgatca cgaacgacga
    14881 attctgctcc agcaagtgtt tggtctgatg taacgtcacc atctacttta acaaatcgtt
    14941 tgcccccagt aacgacatcg actgacggtg gtgtttgatc atttgtatgc ccgttatcga
    15001 catttgcttg gttagaaaat cctttggttg gatctgcttt ttcgttcaaa tgcatatagt
    15061 aaacgaattt caatgtaccg ccaggagtta atgaaggaat ataattcgga tcaactgaaa
    15121 ccgtgaatcc gtctgtttgc tcagtgacag aataatttac tgggtcgatt tctttatttc
    15181 catcatataa ggcataagca tacgttcctg aagaatcatt atcaaatgtt aaagcagcgt
    15241 catgagtatc gataagtttg aatgttgtgt acttgttttg cgttccttct ttatcagcga
    15301 tacctaatgg gatattgaca gaaatctcat attgaatttt ttcaccgatt gcgacatctt
    15361 ttccattcaa ttgaggtgtt gttttatcaa ctttagacgt atcatttttg atggtctttt
    15421 ctactggtgt ttggctattt gccaggatct caaaaggcgt gattgtttgc ttttcaatca
    15481 tttccgcatt atcaggagct tttacttctt ctaaattata ttttccaact tctaaatgat
    15541 taacgatcaa ctggcctttt tcaatagatg cctcggcaaa gtcattgttg ccgatgtcat
    15601 aagaatgacc agtaatgaaa tgtttggctt tggtttgatc agttgtccaa gtgtacaatc
    15661 catctgtgac actttggatg tattttttga cgcttggtgt tccttcttct ttagaaataa
    15721 taaattctgc tccatttagt gcttcgtttt cggcagtacc gatttttgta actttcaacg
    15781 ttccatcatt accgactgta tttttagggt agagatggat agtatctagt tcttctgtcc
    15841 cgtatttata agagccatct gcttgtttga tcatctcata tacagggaaa gctaaaacca
    15901 tgtttgcggc agctgacact ccgtcttttg gttcttcttt gatcgtgtag actgcatctt
    15961 tcccattttg ttttttaggt aaagataaag tgacatttcc atcagcatct gtcgttcctg
    16021 aagcaactgg tgtaccagga gtcaaagact ggactgcttg ttttgctgca tccacggacg
    16081 ctcctttatc tcgttgcgca taaaattctt gagtgacgtt ataaactgaa aatgaaatat
    16141 cggctaatcc ttggtattga tcgaattcgc tcatttcttt cccgctgttt tggattaaag
    16201 gatcgggtaa atcagtcatt ttctttttat gaaggatgac ttgagctgct gtctcctctg
    16261 caaacacttt ttttgcgccg aagctgtttg tgagtaatgg taaaataagg aaaaggactc
    16321 ctaacataac gtttattttt ttatgatttt tcattttttc actcctgttc tttttttgat
    16381 aaacaaccag attgcaaagc ttgcgaatac cataccaaca acgcctaaaa gtgtattttt
    16441 cgtttcgttt gtttttggaa gaaatggacg gctattttct tttttattga ctactttcaa
    16501 tatactgtcc gcctctttat cctcagaaag aaaagttgaa atggtaaacg gaatttctgt
    16561 ttcacttaaa atataaccag aaggtgcttc gatctctttt aatcgataac ttcctgtttt
    16621 caacccggaa attgaaaaag cgccattttt atcggaaatc aggtgaagcg catctgattc
    16681 gttctttgtc cacttgtatc cgttggctgt ttgctggagg taatttcctg ctttgttttt
    16741 aataaggaat actgcgccag ctaatcgttt atctggttta tttgtctcga ttttgacaaa
    16801 ttgcttactc cctgtatata cagagacttt ctttgtactg acaccaaaac caaaatccag
    16861 tgttccgttg ttgttgattt ccttgttcgc ctgtgctgta ctgcttagct gcatctgata
    16921 agaaatggtc agtttttttc cagcatattt ttccaacgaa gggatcgaaa ataaaacatc
    16981 aaatccgtga ggtttcttgt gtatgacaaa gccttctgtc agcttttctc cattcgatga
    17041 aatcgttaaa ctattaggta aaaacgttaa tgcttcatcc gcactatctg acaattcgaa
    17101 cttttgatag tcaaggatat ttacaggaat tgtcgtatgc aaggaataag tgattttttc
    17161 accaatcgta aaatcatttc taggctcttc taataccttc tcaaaaggtg gatcagggta
    17221 gtcgttttct tcatttttag gatacaaatg gatagttgaa agtttttgat tgttttgtcc
    17281 atgtacagga agaacaacta ccatattctc tgccttttct ttgacgactt ctggtacttt
    17341 ggattcaatg aaaagatacg ctttatctct tttttcttga tctttagcgg ccagagaaaa
    17401 agaagcgata ccatcttcgt tgttaagagt tgtagttgtt gcttctgcgg taaacatacc
    17461 ggaagacgca ccgttttttg cgatctctgc ttgtgcttct tctaccgttt tgcccttttc
    17521 ccgtagatgg taaaaagaat ctgtgacatc ataaacttgg aatgtgacac catttaatcc
    17581 tcgatacgtt tgtaataaag ctttttcttg tccgtcattt ggatgatttt tcggcagttg
    17641 cccgtttggg aaaagcaatt tgtgtaaagt aatctgaacc atttctgttt ctgtttctgt
    17701 tgctacctga gtaaaagctg gtttaaatag taacaagaag agacacatac taagccaacc
    17761 aagttttttc atatttagct gccccctctt tcttttttca aaaaaagata ccaaatagaa
    17821 aacgcacaac caatcatccc tactagatag attcctaaac ggccaattcc tcccgtttca
    17881 ggtaatggta cttttgcctg attcgtgatg tctaaagaaa tctggttgtt tttggctcct
    17941 ggtgacagaa cagattcatg atcttgtcca tccacttgaa ttgacccatc ttcgtgtata
    18001 actatagtaa ctggctcttt tagaccttgg tatccttctg gtgtaaaagt ttcggtcagc
    18061 gtataagttc caggttttaa attttcgaat agaaaggtat ctgtttcttg tccatctttt
    18121 ggcgattcta agtccatttc tggtccctgc agtctgaatt ttgctccttt taatgtctgt
    18181 ccgttgtctt cttttttgtg taccgttaga tcaaacgctt ttagtcgatt ttgatgagtg
    18241 agcgtccata cattatttgt catctcggta ttttttcccg tgtaaatgat ttttccatat
    18301 ttatcgattt ggaattcata atttcccggc gaagtatcat atccggcagg accagctgtt
    18361 tcaacgactc gatatttacc aggtgtgagt gaatcaaaac tggcaagacc tttttcattc
    18421 gttgtttggc tgtcaattgg ctgataagta ccatttgctt cttttttctg tagggaaaat
    18481 gctgctcctt taagatttat ctctttgtcc gtcccttgca tcgcatactt acgtactgct
    18541 acaggaactt caacaaaagg attttcaata gtcaactgga tcgtatcatc ggaagtagtg
    18601 actgtttttc catcaatggt taccgtacca tcagaagcga tcttgatttc ccaagtagtc
    18661 tttttgctta atccatgccc ttctggagct tttgtttctg tcagcgtata ggtcatttct
    18721 ttttgcaatt tgacattttc tggaagagaa taggttccgt cgccatgatc tgttagtaaa
    18781 gtatcaatag aatctcctgt taatttgaaa acagcgccaa taagatcctt ttcacctgta
    18841 gaggaatttt tcgttatttt caagtttaac ggtgtgaatt gtttagtatt tttccatgtc
    18901 attgcatcta tttgttgaga atcgtatcct ggtacaggta attctttgat tgtttggtaa
    18961 ctgaatgctt gaccttgatt attgtattga ggtagtgata atatctcttg ataactttct
    19021 ccgctatttg cagataattt gtcaatgtct gcacgttccc acgtatttgt tgtatcttta
    19081 gctggtttaa tgattcgaat atatccgttt ttccaagctg cagcatttgt cgtatgttcc
    19141 cgttgaatct caaaagtaac ttgatctgga cgatcagcta gattgttgtc aaattcttcc
    19201 cataactttt tgatgtgaag actgactccg ggagctttag cagatggtat accgaattca
    19261 gctaattcat tggtatcaat gtttggctgg aaagttgtcc tgccgttcat ttgataccag
    19321 aaatttggat ggaagtcctc attttctgtt tggattctca cttggtaatg gatttggatt
    19381 tcttggtctt ttcctaaata gatctgattg ctcttgatgg tatttccttc tatggaaata
    19441 gatggagtga ccgttgtagg acttgtcccc acactcttga ctgaaagtgt accaggctga
    19501 taaatgaatg gttctgcgat tggatcattt atttgtccat tgcttacagt tgctgagatc
    19561 tgtacagctt ttttggctag gtattcagag atatctgttg catgatcagc tgattcatag
    19621 taaagatcgc ctttttcatc tgatgaaacc atttgtcgca tacgagactc tacttctgct
    19681 tttgagagac cagctgccgg atcgctttga agttggatgc caagaccatg tatttcgatt
    19741 cctcgttctt tgagtgccat cgcttctccg atggttgcga taaacgtact gtcgattctt
    19801 ctggatagat tgttttggtc aggtgcatca tagattcttg agattagaga agtatttccc
    19861 ggccgatctt gcgtattaga aaactgagtt ccgtaataat tgctgcttga ttgtgcgtgt
    19921 acccgctgta ctttatagga aaatgttggt acaccatccg tcagcaaaac gatcactttt
    19981 ttatgtccat ttggaacgga tagcatgctt cctgcatctc ttagtgcttt ttgtgtaaaa
    20041 gtaccaccat ttgtccgtga aggtgtaatg gattttactt gatttttcac tgaatcaaat
    20101 gaacccatct gtactgcacc gttactgtag ctatatcctt cgcttgagta gccgacatat
    20161 cccatattga ttttgtctgt gataccgcta tctgctaaag tatcgacaaa acgatcgaca
    20221 ccaatcttta cttcaccgat ccgattattg tcgttcatac ttcctgacca gtctacgacc
    20281 aatacgagat caagaggcgt gatatctttt tgtacatttc cacgagcatt caaataaaca
    20341 tcaaacaatc cgggtgtact tgtttccttt gcatactttc gtatcgcata gtctgcttga
    20401 ttaccggttc ctccatattc gatatacgaa tgggtccgat cattaggatc tccattccaa
    20461 gaatttatac catcccattg atttgttccg tttttattgc cttgatgatc aagaacattt
    20521 gtatttcctg taggttgcca actggcttta ggataagtgc cctgatcatc tgttgtatat
    20581 tctgtaggaa tcgtttgatt agatggcaca ctcacagctg gcaaggataa tgtaggcaat
    20641 gtaaatgggc tgacagaatt ttcgttttct tttgatgatt cttcttctat ttcgtctggc
    20701 tttggtactg acacatctgt atctttctta ttttctgtct catcaataat gtcatattct
    20761 ttattatcga cgatcagctt gtttgcctgg ctgtggtaaa gtgctggatc gattggaaaa
    20821 ctcagatcga tagtttgttt agtgtttgat gagatttcca acgtaatttt cccgctattt
    20881 acctgataag catagccatc tccttctttt ctttccacat cacttaagta attttcgact
    20941 agaccaattt ctttttctat cttttcagca ctggtgttat tcgcatttag cttgatgttc
    21001 agtgcttttt ttccagactg atccgaaatg acttcagctg aaattgtttc taaaactgtt
    21061 ggatcatctg tagtgtctgc tattgcagat aatggcacaa agtttgataa actcaatatt
    21121 atcagcatga aaataacttt cagtttcttc cctgtggtcg tcatttttcc cctccttttt
    21181 ctttttttct gttttgacgt gaatagtgaa aattattaaa ataaacaggc gttacataat
    21241 tttatttatt ttaatatgat aattattata cgaagggcgc taaagggaat cacaccaatt
    21301 ttcataccaa ttggtttcat ttatgaagtt gattccttcg attaatgagc gtttcattga
    21361 aagaaaaggg ttgagggaga aaaacatatg atcaggttgt tggaaggaaa ctatagaaag
    21421 atggaaaaaa tcattcgaat tctgtccatt tctgacaagt ggatgactaa aaaagaactt
    21481 gccaaacgaa ttggtagttc agaatctaca tttgtaaggt atatcgaaga gatcaaaatc
    21541 cgctggggaa cggatgtaac gattcaatct tcacaaaaat tggggtatcg tcttgaacat
    21601 ttgaatgtac cagcttattt aaatgttctg atggaaatgt cgcaaagttc tacaaacagt
    21661 cgtttattaa atgagattat tgtcaatccg ggtaaaacga ttgagtatta ttgtgaaaaa
    21721 atttccatca gccggtcttc ttttacacgt aagttgaaac aatgcaatca aattttagaa
    21781 agctatttat taaaagtgat cgttgaccaa ggttttcagt taactagcct taaaagtgag
    21841 ctccagttgc gtatctttgt tacctttttc tttttaagtt actacggaag acgcacgttg
    21901 ccatttccaa tcgaaaaaaa gaagatcaaa gaaattatgg tcaagaataa ttgtcagatg
    21961 aatttgtttc aggaagaaag cccatatgag caatctttct ttattatgta ttttgttgtg
    22021 catctcatga gagaatccca aggaactcca tttgaatgga aagctggaga ctcttatgta
    22081 cagttgataa atatcgataa aaaagacttc ttaatcttga aaaatctctt aggaaaattg
    22141 aagtacagca gctatataaa agtagtgaac tattttgtca atacgatttt ttcagaattt
    22201 tcggagaaag aaaaaaatga aatcagtgag cagatacgaa aaaatatgtc ctactgttcg
    22261 ttttcatccg ctgattgcaa agaaaaaaac gaagaatatg ttgttcaatt attaacgcaa
    22321 atttattttt tctcccgtat tttcccatac gatacgactc atttgacgat ccgtgcaact
    22381 tactttgcac agcagatgca acaccgccag atacgtctat atgctggatt tcgtgcagaa
    22441 cttcaatttt attccactct actgaatcag aatctgattt attatttatc tagctatatc
    22501 ttttggatgc ttatcggtaa tcctgaaatt caccattata cttctgataa aaaaatactg
    22561 attatcagcg acttaagtct tcgacatagt caatatattg aagaatatat cagtgatatt
    22621 cttgctgtgc ataaaattca ctctgagaca actgctatta ctgaagatca gctgtcgaaa
    22681 tacaatcttc tcgagtatga tctgatcgtt acgaatcaac ccattttgaa tgcccatgtt
    22741 cctcatatat tgattgatga tagtgtgtca ttcgcaaatg aagaagaact catccgctta
    22801 ttcgagttat gaataatcaa aaaacagcta tgtataaacc accctgataa aaatttcagg
    22861 acaaatacac atagctgttt ctttttattg tattccttca gcaatccaac gagatttata
    22921 gcgaaagtct tttaacaact cttcttttgt ccaattattt gcaatgagat ccgtcaaagt
    22981 gttcattacg agggtcatgg aaaagtatag gaaactaact gcttcttccg acgattgaat
    23041 ggagaaatga ttgattctta ataattccag ccatttttcg ttcattggag aatcagccga
    23101 tagttctcta cgtttggttg tctgctgatc atttcgctgg gttaacaagt ataacctttg
    23161 ccaatagata ctttcgtgat ctaagtcgca gcaccactct aaatattttt cgatccccaa
    23221 aaagaaatgt tgtttgtttt cgctaatgaa ttttaagata tcttgatgga ctatgtgaga
    23281 actgcttttg atcgtataca aataagcatc ttccaaatct tgaaaatatt tataaaaagc
    23341 accgcgcgac atccccatct tgtcaacgat ttctgagact ttgacttggc tgatgtgctg
    23401 tgagcaaaat atctccagta ataggtcatc gattctctgt ttctttgcgt tcgataaatt
    23461 gaaaaaagtt tcttttggca tccacatctc tcctttatct ctgattatac gtggatagcc
    23521 cttcattgtc atgaaggatt cattccttct ttttctcttc ttctgtcaag tacccatctt
    23581 ccattcgata aacgcgatca ctccattgaa tcatacggac atcatgagta accataatag
    23641 tcgctttttg tttctcatga gcttctttaa ctagtaattt gacgacttca tatgcatgat
    23701 ctgtatccaa actagctgtt ggttcgtctg ctaagatcaa gcttggctca ttatataaag
    23761 cgcgagcaat tgccactctc tgtctttctc caccagaaag gtctttagga taactatttt
    23821 taagtgactg gatatctaaa gattgcagca aggaatcgat ttttgttgaa tcttcttttt
    23881 ttgaagtaac ttggtcaact agttcaaatt gttcctttac cgttaaaaaa ggaatcaaat
    23941 tagagctttg gagaataaat cctatttctt taaagcgtaa atctgctcgt tttttttctg
    24001 gaagctcagt tagtgagtga ccgttgatca ctatttctcc ggaagtcggt gtttgcaatc
    24061 ctcctgaaat ggtaaggaaa gtacttttac ctgaaccaga aggaccaatg atgctgacga
    24121 attctccttc ctttacataa aaatcaattg ctttcaaagc tgagaccttt gtatgaccag
    24181 aaccaaatgt tttggtaact tttttcatcg ttaatatttt tttcattttt attaacctcc
    24241 tattgctgta attgggtcta ctttggttat cgtacgtatc gaaaacaatc cgcctagcat
    24301 tgcgacaatg atcaaaatcc cactatatag caaccattgt gaccaaaaaa cagcaaaagg
    24361 catcgcgctt ggtaaaatca gactagtcaa ataagcaaac aaaagagcaa gcagaacgcc
    24421 taccacccca acaataaagg actgtgctac taaagattta gcgatgaaga aatttttgat
    24481 cccttgtgct ttcattactc caaaaatagc agtcttctga agtgtcatca cgtacatgaa
    24541 aataccgacc acggcagcaa ccactaagaa caaaaagtat atcatcgcat ttaaggtcat
    24601 attttgggca gaatagccag gaatactttc aatgaatgtc ccaatagtta ataactgtag
    24661 acccttcgat gctttttctt tcgaaatttt agctgaatcc tttattacga tcccattgat
    24721 cggctgttct tcttcagaag caaacggctg ttgtccatac ttcaaagcag tccacgtatc
    24781 tagggacgca tagatgacgg gagaaactgt gtaataagtt tcaggaaaga taccgacaat
    24841 cgtcaaagga tcgtcgtaac tgccaatttt gatcgtatct ccaattttat agcctgaatc
    24901 agccagattt tgagagatga tgatttcatt ctttgtttta aattcatgcc ctttcgtgag
    24961 ttttggtaat aaaaatgctt ctttcgttgt gccgaaaact gtaacatttt ctttgttttt
    25021 tcctttgatt gctccgctgt acaatccgat tggtgctttc tctcctcctt caatgtattt
    25081 cagatctcct cttgttaatt gtgaagctgc aaatacttga tttgcatcct ccgacaaaac
    25141 aatctcttgt gcgtcccaat catcgattgc ttttttaaac tcttctgaaa gtccattcgc
    25201 tagtcctgac aacatgaata cgacatacgc aatcaagaac ataataccga cgatcaagcc
    25261 atatcgtaat ttagaatagc gaatttcttt gatagctaga aacatttgtt tttcctccct
    25321 atgatagatt gatagtgaca aaatgtcact tgttgaatag tgtaactcta aagtgacaat
    25381 ttgtcacttg tgttgttttt ttcttatcaa taaattgtct aagacatctt tcgataaaac
    25441 cttaacatga gtcataaaaa atttatttaa gccttattta tgctttccat gataaactaa
    25501 agtgaccaaa tcaatgaggt gggaaaatga ttaaactatt agtagtagaa gacgataaag
    25561 tattatccaa taatataaaa gaaatactcg ctgcaatcgg cgagataaca caagtttatg
    25621 atggtagtga aggactgttc gagttggaaa gtggagttta tgatcttgca atcttggatt
    25681 tgatgatgcc ggaaatgaat ggatatgaag tgttagcaaa aatacggaaa gaaaaaatac
    25741 agactccagt attgattttg actgcaaaag atagtttgga agataagatt gaaggatttg
    25801 aacgaggtgc agatgattat ctaacaaaac ctttctatcg agaagaactg atcatgcgcg
    25861 tgaaagcatt attgaaacgc tcgctaggtc tctttgatga aaatgttctg aagtataagg
    25921 atatcagttg tgatttaaaa agcaatgaag tcttgtataa agaacaagtt ttgcccatac
    25981 aaggaaaaga gtttgaattg ctcgtttatt ttttacaaaa taagggaatc attttgacaa
    26041 aagagcagat atttgaccgg atctggggat ttgattctga tacgacgatt acagttattg
    26101 aagtttatat gagcaactta cgaaaacatc tgcgaagtac tggattggat aaagaaatca
    26161 aaaccttacg taatgtcggt tatatactgc aaagggtgtg atgcaatgaa tggagatatt
    26221 acacgaaagc aacgtagtcg cttttttgcg acaaatgtag cagcatttgc ttttatcttt
    26281 tttcttttgg gattcattac tttacagata ttaaaccaat ctgcatacag agaaacagat
    26341 ctgtcattag agaaaataaa ttttgattct cgaacagtac aattagagat acaaagatat
    26401 cagcaaggcg atcccttttt aaacccgact caaacggaac aaaacagaac aaacatggaa
    26461 cagccattaa tggaaccatc aaatgaccgt tttaacactc aagtgatctt gtggtctaaa
    26521 aatggtgaga tattgaataa agcagctatt ggtgggcgtt tcaaccaaat caaagaatta
    26581 aaaatggata cgaaaaatct gaataccatc caaaatattg aattaaatga agacagtggc
    26641 tcatctgccc tctctttccg ttcgattacg aaaaaagcgg agacgaatca agatggcgtt
    26701 gcctacatcc aaattttaga aaatacaaat caaatcaaga attcattagc gacttttcgc
    26761 acgattatga ttttgtgtat gattgttttt tggattctct ctattggtat cagttattac
    26821 ttgtctagtc taagcatgcg ccctattatt ctttcttgga gaagacaaaa agaatttgtt
    26881 gagaatgctt ctcatgaatt gcgcacaccg ttaacgatta ttcaaaatag tttggaacat
    26941 ctttttacca agcctaatca tactattatt gaagaatccg aaagcatcgc tcaagcattg
    27001 tcagagacga ggcgcctcac tggattgact tcggatttgc tgacaatcgc tcggaatgat
    27061 tctgatcagc agctgctttc aaagcaaatg atcaatacac aagaatatat cgaacaactt
    27121 gttaaaccat ttcaagagat ggccatcata gatggaaaag agtttattct ccataacgat
    27181 gcaacattag aagtaccaat tgatgaaaaa aaaatccatc aagttttggt tattttgctg
    27241 gataatgcat taaaatatac gaatccagaa gataagatca ttttggattc atctgttaac
    27301 cataaagaat ggctgattca cgtaaagaat actggccctt ctatcagtga agaagatcaa
    27361 gcaaggattt ttgatcgatt ttatcgggaa gatcaatctc gggcgaaaga gactggcgga
    27421 tacgggcttg gcttggcaat tgcaaaacag gtaatcgaac agcatcatgg acagatttct
    27481 gtatccaatt atcttccaca aggagtcgat tttaaagtcc aattgccgtt gaagcctaaa
    27541 aaataatgct tatccctaaa aaagggctgt gacaaaaatc ttgtcacagc ccttttttgt
    27601 tgcgaataaa cggtgttcca gaagtagctt cttcggaaat aagccgaaat ttctttaagc
    27661 tgaccacttc tgtcacaacc tctttagctt acgcttacga ttttttattg cggtcttcaa
    27721 tgtgtacatc accgcttgtg tcaatatcca attcttctcg gctaacatct tctgtcactt
    27781 tttcaacatc ttcatgttct tctttatgaa tcccaacttc ttcacgaata actgtatgtt
    27841 tgcttacatc tacttgctct tctttgattg ggatagtaat cgtgtcatct tccaattttt
    27901 cgtctgttcc ctgtgaagat tgatctgtta ctggtttgcg ttcgatgacg atttcttcac
    27961 gtttgaccgg tacttctacc gtttcagtat cgttgaccac atgtttgtgg atgtcgactt
    28021 ctccagttgt cacatcatgt gtatttacat ctaatttttc ttcttttagt tgaattgtat
    28081 catcatctag atttggtttg cgatcgatgt cttcagtctt gcttgtaccc attgtatcag
    28141 tctcgccagg tacgtccatc ggatcagttc ctggtgtcat attcaatgga tccgttgtat
    28201 tttgaaagcc tgtcgtgcca cctacgtcca ttggatctgt tccagtgcca gtttctaatc
    28261 cagtccccat tgtgcttgct gggctaacag tatcttgtgt atccattgta tcttctgttg
    28321 tttctggctg acgatagcct tttactgtga tgacaaattt tccatcagaa atatctcctc
    28381 tatattgata taaaggatca ttttctggtg tagccgtctc agaatcatag gtatcaaaag
    28441 aaaaagtatc ttttattttt tcccataaag aacggtcttc ttcaccatcc atccgatttt
    28501 catctgtttc aacatctata ccagacaatc cgtcaaatcc tagtctagat gctgcttcat
    28561 cagtagtata aataacgata tcatctcgtt catacccttc gttacgcaac cgttcaatta
    28621 ctgataatgc ttcttccctc gtactatagc ttccgatgac cgttaaattt ttctcttcca
    28681 tgtaaattcc tcctagcaaa tagatttatg attggtatat tgttacttat aaatttattc
    28741 tataggagac gattgttttc tacaaatatt tagattggaa tttgattaaa acaatttaat
    28801 tgaaatttga aaacatttat gttaatgaaa gtcatattct aataaaatgt catttgtttc
    28861 tatatgaatt tttatgaaaa atttcatata atttccactc gttgtgatgg tttaatttat
    28921 ccaatccatc aataagcaaa caacttatat aaaaaaggat taattctcaa tataattgct
    28981 cctaccatag aataaatctt catgtaaagt aatgaagtac caactatttc taatacttct
    29041 ttaagaatta tcccaacaat atcgttagac aaaccattat tgaataaatg gagagccaat
    29101 cgattctgaa aaaatgtttt ctaatttttc ggaaataaaa gaaagagcct cctgccatta
    29161 gaacgaacca aatccatttc attgctcatg aagtaaccac atagctaaaa agaggataaa
    29221 tcaattaaaa gatcaaaaga atgactgcac taataaaata actaaaaatg tatcttctta
    29281 cttcatatct tttagctagt acttagttaa aaatttgctt cctaagatca agacaaactg
    29341 tctatcttca tttgatgaaa tgcggtaaaa tatagaaaat gaacactatt ggagggaatt
    29401 tgtaatggac agcaaaacta gaaaacaact gcaagaaaag ttaaagcaac gtacacactt
    29461 gctcccttcc gtctttcaac agtttttaga agaaaatcgt tcttcccttt cattaggcag
    29521 tcgctatgaa tacgcaaaag attttcatct gtttactgat tatttgagaa aagtattttc
    29581 tgaagaagtg gatgatcagc tgattatcca tttagaaaaa caagattatc agaatttctt
    29641 agctaaaatc tatcgacata ctcgttcgtt ccaaactaca gcgaaccaag aaacggagca
    29701 attgtttgaa aatagctatt acgggatttc aaggaagcaa tattcattaa atcatttttt
    29761 acgatttttg tatcaaaaag gactgattga agaagagatt tctcttttgg gtacaagttt
    29821 accggaaaca accaaagcag ctccgcgata tatcgatgca gaacttttca aagattttga
    29881 gcatttgttt atcccgattg aaggctttca aacaagcaga gaagctagct ttgaagaaaa
    29941 gaatagaccg aggaaccttg caattactgc cattcttctg tattgcggat taaagatcca
    30001 tgaagtagta gaacttaaac gtaaagatgt atctttgacc aagcagattc ttaatctgaa
    30061 tagaaaagaa aacagactga ataaagtacc aataccaaaa gaagcgatgc catttctgga
    30121 gaactatttt aagttaacag cacaaaatac ggacgaaaat tcttttgttt ttcgttcctc
    30181 acatgatcag caaatttctc ctcggactat tcgccaaatt ttgagcaaaa ttacagtata
    30241 taaaaacgta tcgccagaac tatgcagaaa aacatatcgt tattatgcag aactatattt
    30301 agatgatgct gacgcggtaa attatttatg tggtaatcga tacctctcat cccattcttt
    30361 tcaagatatc tgggaaaaga tggttgattt ttcttatcac gaattagcgg cattcgttcg
    30421 ggtttcttcc atataaaaca aatgtatgat ctttttttca caataaacta tgttattata
    30481 taaaggatta tgttacgatt atcttgaatt tgtatattac ttaacaaaag ataatggagt
    30541 gtgaatgatg gagaccataa cgtattcccc tttgaactta tttacgaatt ttcaagaagc
    30601 tgcagctaac ttttccgaga ccccgattat ctttgatgag ccactttcag ctttcccaga
    30661 attaggactt gaaacaactt acaaaaaatg ctgcgaaagt gtgttgaaac gggcttatca
    30721 attggctcat cttggagtga aatccggaga caaagttatt atttataaga gttccgcttt
    30781 tgacacatac cttctagctg tttctgtgtc ttaccttggt gcagtgcctg tcatgacttc
    30841 ttatcatcta ccaacaacaa caatggaagt atttatcgat cgtttagaag accctttcat
    30901 tttatttgat gatgagacaa aaaagcgagt gagagaaatt tcaaatggaa caaaatcaaa
    30961 acagattcct attctttatt taatggaaca acctgctttg tctgtttctc aatcattttt
    31021 agacaaaaat gaaatttctt atatgaccca tacttctggt acaactggta tccctaaact
    31081 tatttgtcat tctgctcatt ctatgggatg gcggacgaaa tggcaaaaaa cagttttcac
    31141 aaaaatttcg gaaaagaaat taatcgcttt tcacatctct ccagtgcatt ccagattcaa
    31201 tattggtatc tcttctttga tgtccatggg atttcctatg atgcctttag caaacgcaca
    31261 aagttcaaaa gttgttcata tgttggaagc tcaccggcca attgcactag agacccatcc
    31321 gaataacttt gttcagtggc gttttactgc taaggagcat ccagaagctt tttcgggtat
    31381 ccgatactat cactccactt ttgatgctat aaacaaccag acaatgaaga catttttgcg
    31441 aacgtcgatg gcacaggatg cggtatttct tcaagtatat ggccaaagtg aatgcggacc
    31501 aatgattctt aaggctcata cactagagag tttgaaaaca agtgatgcaa gagatatggg
    31561 cgttggactg gaagacatga caagcgctcg gattacagat tcagttggga atgtcctacc
    31621 tgaaaacaca gatggtcata tacaattact gtctaaagga cgggctttga cttattacaa
    31681 ggaagatgca cggttccagg aaaatgttta tggcgattgg tgggatagcg gagattacgg
    31741 ttttatggat aagcatggcc acctattttt gaaagatcgg caagtggatt tgatcgaaac
    31801 gatcaatagt acattagcta ttgaagactt tttattagat tcgttagatt ttttggcaga
    31861 agtagtgatt gtaagggata aaaatcattc tccacaaccg attattgctt tagctcctga
    31921 taaagaaatg gattggaatc gatggtggga gcaagtccat gaacttcctc gcttgaacgt
    31981 ccctatcatc cgtgattttg atgcaatccc gcgaacagct acaatgaaag tgcaacggct
    32041 tcaaatcgag aaagaattaa aatcacaaaa gtcctaacta tttaaggaaa gaaagatcca
    32101 ttttggtctt tcttttcttt ttgttttttt aaattggatg agataaaaat atgccaattg
    32161 gctgtttttc ttttttgaaa atttcgttat actcttattc atggaggaaa atgttatgaa
    32221 aagaatcttg acaattggcg gttctgatcc atttgctggt ggaggaatcc aaacagattt
    32281 aaaaactttt gagaattttg gtctattcgg actaagcgcc ttgacttcta taggggcact
    32341 agatattaat gagcgattta tgctggaatc tatatctttg gaattgctta aacgacagtt
    32401 agtttcgatt gataaaatga cttcattaga tggtatcaaa attgggttat tgaattcaag
    32461 tgaagcaatc atggttgttc gagacttcat aaaaacaaaa caaaatatcc caatcatttt
    32521 agaccctgtt ttggcattca aagaaactga agctgtgatc aatcaattgt atatttcgca
    32581 gctcatcgaa gaactttttc cacttgttac gatgattacg cctaatttgg cagaagctgc
    32641 tttactttct ggtcaagctc ttccctcttc gttaacagaa atgatccatc tcgctaaaga
    32701 actcactgct tcaggaacaa aaaacattgt gatcaaaggc ggcacaagga tggctggaga
    32761 tgaagctgtg gatcttctct attcaattga tcattatgag gtattttctc ataaaaaaat
    32821 cgacactaca acagtaaacg gggccggttg tgccttttct tctgcaataa cagcgaatct
    32881 tgtgttaggg gcagatttta ccaaagcagt agcagaaagt aaaaaatttg ttgcccattg
    32941 tatttctaat ggcgtgctga tgaacgatca taccggaagt gtctggtctg gcggtattca
    33001 aaaaggagga atgagaaatg aaaactaagc aattaacgat ctatgctatg ctgattgcgt
    33061 tgacagttgc cctttcatta acaattttga tccctgtacc ggctacgaat ggatttgtga
    33121 cattatgcga ggccgggatc tataccgctg cttttcttta tggtccaacg ggtggattag
    33181 ttgttggtgc ttccagtggt ctattgatcg atttgatttc aggatatccg cagtgggcag
    33241 ttttttcatt agtgatccac gggttacaag gatatgttgc tggttctttc agcaaatctt
    33301 ctactcgtat gtggggatta gggcttttat taggaagctt agttatgatc atcggctatg
    33361 gtatcgccgg ttggatttta tatgattggc cggctggagt ggcttctata ccaggaaaca
    33421 tcttccaaaa tctgattgga atgggacttg cctttcctat aactgcaagt cttcaacgta
    33481 ttcaagcacg gaagcaatat taattgaaaa agaaggtttt agaaaatgaa gttgtcagaa
    33541 aaagaattaa aagaccaatt gactgaaata gttaatgaca tattggctga ggcacacttg
    33601 aaaaaaggtg atctttttgt tttaggatgc agcacaagcg aagtcgttgg aggacatatt
    33661 ggaaaaaatt ccagtgcgga agttggtcaa tggatcatcc gtacgttaaa agagttgttg
    33721 gatcctaaag aaattgcgct agctgtccaa ggttgtgaac atttgaaccg agcattagta
    33781 gtagaacgaa cagtggcaga agcaaaaaat ttcgaaattg tctctgttgt tcctgcacta
    33841 catgctggag gtgcttgttc tgttgctgct tttgaccagt tcaatgatcc tgtcgaagtg
    33901 gaacacgtag ttggacaagc cggtatcgat atcggtgaca catcaatcgg tatgcatatc
    33961 aagcatgtcc aagtacctgt cagacctcgt ttgaaaaccc ttggacaagc tcacgtgaca
    34021 gcccttcgct ctcgtcctaa atatatcggt ggtcctagag cgaattattg aaatgagtaa
    34081 ttgacagagc aatctgaaaa acttcctcgc aaagtccaac taatgaactt taacgaggaa
    34141 gttttacttg tttgttttat ataaccgagc ttcataaggt gctagtatgc ctttagcttc
    34201 aaattctccg ctagaggaat ttgacaagag taatgtccag gactgatctg cttcttttgg
    34261 aagttcatag tcagcaagtg attctgttaa gtttaccata ataagatatt gttcatcttc
    34321 taaatatctt tcatagacat aaagttgtgg atgttcatga agatattctt tataagaacc
    34381 gtatatcaga cctttattgg tttgtctaat gcggatcatg ttcttataga attgcaggac
    34441 cgactctggt tcatattctt gttgttctac attgatcgtt ttggtatttg gattagtgat
    34501 cagccaaggt tcatgtgtag aaaaaccagc atattgttcc gaagtccatt gcatgggtgt
    34561 cctgctgttg tctctcgttg tttcacgaat gatatccaat gcttcttctc ttgtcttacc
    34621 ttccgcaagc agccttttat aaagtctttt cgaatctact gcatcaactt gatcgatcga
    34681 ttcaaagggc atattcgtca ttccgatttc ctgtccttga taaataaatg gcgtaccttg
    34741 caaaaagaaa tacatcatag cgattgcttt tgcagacata gcccaaaaat cgggacgagt
    34801 atctccaaac actgaaacgg cacgaggaac atcatggttt tccatatata aagcgttcca
    34861 tccgatacca tccaatgaag tttgccaagc gcttaatgca tgtttcaatt taaggacatc
    34921 taattgccct tctttttctt gctgccagat atggatatga tcaaattcga atatcatatc
    34981 gaaataaccg tctttcccta cccattgtgg tccttcttct gcagtgacac cacttgcttc
    35041 tccgacagtc atgatatcgt actcttcaaa aacatgtttc aaatcggtca aatgttcttc
    35101 gattcctgag acattttgaa agggtgagaa agggttctct gtggctttta ctgaatattc
    35161 atcttttttt acatgagaaa tcgcatctaa acgaaatcca tctatccctt tatccaacca
    35221 ccatcggatc atattgaata gttcttcttt caatttctcg ctttcccaat ttaagtctgg
    35281 ttgttctttt gcaaaaacat gcagataata ctgtccagtt tcatgagaat actcccaagc
    35341 agaaccgccg aaaatcgatg tccaattatt cggttctttg ccatctaccc cgtcgaccca
    35401 aatgtaataa tcgcgatacg ggctttcttt agattttttc gattcttcaa accattgatg
    35461 ctgatccgaa ctatggttga tgacaagatc cataatcact ttgatcccta attcatgtgc
    35521 ttcttttagc aataaatcaa aatcttccat cgttccgaat ctctcttcta tatcttgata
    35581 gtcacttacg tcataaccat tatcgacaaa tggagattga tagatcggat tgatccagat
    35641 aaagccgata ccaagatctt tcaaataatt aagtttttca cggatcccat tgatgtctcc
    35701 ttgaccatcg ccattgctat ctttgaaact agcaggatag atttgatagc ctactgcttc
    35761 tttccaccat ttttgttctt gtatcatatc gtttcactcc tttaacggtc cctcttgatt
    35821 ataaccgatt ctattttaga agtatccttg ttaccggtaa ctagaaacga aaagctcaac
    35881 gaatctgcta agtgcgttcg ctgagcttta ttttatcata gatagcgggc aatcagttga
    35941 atcatttgct tgggaaattc attcaagtca gtaatatcta caaaacgact ttcaccgtag
    36001 atcgacttga tttcttcttt gtcttggcca atagctgcgg agacaaatag gattccttga
    36061 cgttcatatt ctttcaaaac agactggata tcttcttttg cttttttgcc tgtataatcc
    36121 ggcaacgctt ttggctgacc gtcactaata ttaagcaaca gtttggttgt tgcggattgt
    36181 ctgtttaatt tttctgctgc taaacgaagt gcagcaccat cacgattgtt ttgccttggt
    36241 ttcatagtga ctaatttttc atttaaccat tgaaatccgt cttcaaattc tttataagaa
    36301 aacaaagaag tcttttctct tgacgaacgg tctgctgtat ctccgtaaat caacaatggg
    36361 atatcgactt tcttagaaaa ttcggcaatg gcaatcgctg ctttttttgc cgcttcgatc
    36421 cgatcatccc gtatcattga cccagattca tcgatccgta cagctaccgc taaagaaggt
    36481 tgctcatgtg gcgggttctt tttgtcaaaa tttctcaaat caccatatgc tactttgctt
    36541 gcattgaacc gctgaccttc gtattttcct ttttggtacg tctctgtttg ttcattatcc
    36601 agtaactcta aaatttgccg gctaagtgat tctactattg gcattacttg catgtgtagc
    36661 tcacgtgctt ccggcgtttt tccttggaaa gctggtcggt gaacgatgag accttctttt
    36721 tcatgaatcg tttccttcat gatttgtcgc gcttctttat tcagttgctt cttgatttcc
    36781 ttatcatgtg cttccatgtc agaagcagac atttcttgag aatcatctcc tgtttgttgt
    36841 tctttttgat tttttccttc gatatcgcta ctgcgttctt tttgcatttg aggaggaatc
    36901 tcgttttttt ctattgtttc ttcttgtttc tcttctccct catttggttc ttcagcagac
    36961 aacgatttct tttcatctaa gcgcagaatt tcttcagcat tttcagtgat gtttcgttca
    37021 tttgcttctt cggcgctaat ggttctttct tttgtatgct cagattgagg atgaccccat
    37081 ttttcttttc tcaatatgtc ctgaagttcc agtagcaatc cactgtcaga caaagcttgt
    37141 tcaagaattg ccaattcatc tgaatcagtg gataatttat acaaaacttt tggataaatc
    37201 gactggagta aatcttgtcc ttgcttatag gtatttgccc aataaaaata agaacgcatt
    37261 cctgctacac ctttgatggc gttagctttt gcagtttcat ccaacaaacg gataatctct
    37321 gccatcttta agagaagttc ttgtttttgg atcatagttt tcgccatcgc acgttctgcc
    37381 atgacttcta aggcaggaag atccattttt tccgcatgtt gcatccgatc tcttaaagat
    37441 tcatttaatg ggcgatttcc ttggtaattt cgatttgtag tgatcacaat gacgcaatct
    37501 ggatggcgac gaatgattcc tgtcggtaaa ttcaacatcc catttggttc gagcgctgag
    37561 ttaagtgcca caagaacaga ggcatctcgg atgacagtcg gctcttgaat ctctaataaa
    37621 tagccttttt caatcgctcg aacgatttca gaagggtaat agctgtaatg gattgcatct
    37681 ggataatcag cttcgatcct ttgaactagc tctgctaatt tttctttagc agaaaaagga
    37741 tcaatgccgt aatggttctg gatcagtgtc agtacagctt caagtgtatc tgtttggtaa
    37801 atagcttcta gtaattcttg gtcttgttcc ttattagaat ctactacagg taataaggct
    37861 ccgaaaacat ctgatttgtc catatctgca aaacaggtca cttttgtata gggaagttgc
    37921 agatcagcag atagtgcttt ggctaattgt gtcttccccg acccggcatc tccttccaaa
    37981 agtacgttac tgattttcat ttcaggatct tgccaattcc ctttgatttc ggaggcgatc
    38041 cgccattctt ctgcacttgt ccgatggctt ttcggtttct tccagatcat ttgcttttct
    38101 agccgtgaaa aggttcggtt ttctgttagt gagaaattct ccatgcttct cttccccttt
    38161 tgatcatctc tgtctattaa tatcctagct catctaaaag acgttttaaa atacggaatc
    38221 gttccccaat cagttctccg tctttttgta gtgtatcgta atacaaaatg atgtcttcat
    38281 cgatctttgg atttttggta tctacttcta ccgtcattcg gttcccttgt aatccaatga
    38341 tgtctacgac tggattttct ggatcataaa atttttcata acgataagca tcttggaagt
    38401 ataaacttcc ttgcgcagct aagatcgcta aatctaaaat acgtgtgatt ggtaattctt
    38461 ccgactgtcg tgaccatttt tctccagtat gtcgccatac cttgcctgag atatcgactt
    38521 ttccacgatc attccactga gcaaggccca gtgaaagccc ttttgcatcc atatttaatg
    38581 catttcttcc atcaacttta tcatagtctt ctacaacgat tactggttta tgttttaaat
    38641 ttgttggtat ttccattatt ttaactcctc atccttttat ttttagtaaa taagtaaatt
    38701 agtagtttac tcatttacta aactatatgc gacattatag aggattgaag tgaggattgc
    38761 aaagaaaaag agcttcattg atagaaataa aaacaccatc tcgttctttt acacgctcat
    38821 tagtaacatg tagaaagtcc aagatgatgt tggaat
//
LOCUS       DACDXE010000021        37551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.021, whole genome shotgun sequence.
ACCESSION   DACDXE010000021 DACDXE010000000
VERSION     DACDXE010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.021"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            75..470
                     /locus_tag="IWM23_001078"
                     /pseudo
     CDS             75..470
                     /locus_tag="IWM23_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            790..1104
                     /locus_tag="IWM23_001079"
     CDS             790..1104
                     /locus_tag="IWM23_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883738.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            1208..2386
                     /locus_tag="IWM23_001080"
     CDS             1208..2386
                     /locus_tag="IWM23_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9883739.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            2789..3082
                     /locus_tag="IWM23_001081"
     CDS             2789..3082
                     /locus_tag="IWM23_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAP9883740.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            3925..6252
                     /locus_tag="IWM23_001082"
     CDS             3925..6252
                     /locus_tag="IWM23_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9883741.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            6378..6707
                     /locus_tag="IWM23_001083"
     CDS             6378..6707
                     /locus_tag="IWM23_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9883742.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            6747..7751
                     /locus_tag="IWM23_001084"
     CDS             6747..7751
                     /locus_tag="IWM23_001084"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAP9883743.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            7748..8194
                     /locus_tag="IWM23_001085"
     CDS             7748..8194
                     /locus_tag="IWM23_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HAP9883744.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            8344..9159
                     /locus_tag="IWM23_001086"
     CDS             8344..9159
                     /locus_tag="IWM23_001086"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9883745.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            9365..10537
                     /locus_tag="IWM23_001087"
     CDS             9365..10537
                     /locus_tag="IWM23_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9883746.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10699..11193)
                     /locus_tag="IWM23_001088"
     CDS             complement(10699..11193)
                     /locus_tag="IWM23_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883747.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            11776..12198
                     /locus_tag="IWM23_001089"
     CDS             11776..12198
                     /locus_tag="IWM23_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9883748.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(12364..13557)
                     /locus_tag="IWM23_001090"
     CDS             complement(12364..13557)
                     /locus_tag="IWM23_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9883749.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            13781..14158
                     /locus_tag="IWM23_001091"
     CDS             13781..14158
                     /locus_tag="IWM23_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9883750.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            14146..14484
                     /locus_tag="IWM23_001092"
                     /pseudo
     CDS             14146..14484
                     /locus_tag="IWM23_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            14618..15061
                     /locus_tag="IWM23_001093"
     CDS             14618..15061
                     /locus_tag="IWM23_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HAP9883751.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            15322..16137
                     /locus_tag="IWM23_001094"
     CDS             15322..16137
                     /locus_tag="IWM23_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9883752.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            16147..16809
                     /locus_tag="IWM23_001095"
     CDS             16147..16809
                     /locus_tag="IWM23_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9883753.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            17017..18681
                     /gene="alsS"
                     /locus_tag="IWM23_001096"
     CDS             17017..18681
                     /gene="alsS"
                     /locus_tag="IWM23_001096"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAP9883754.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            18694..19404
                     /gene="budA"
                     /locus_tag="IWM23_001097"
     CDS             18694..19404
                     /gene="budA"
                     /locus_tag="IWM23_001097"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAP9883755.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            19534..20031
                     /locus_tag="IWM23_001098"
     CDS             19534..20031
                     /locus_tag="IWM23_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAP9883756.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            20216..20476
                     /locus_tag="IWM23_001099"
     CDS             20216..20476
                     /locus_tag="IWM23_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883757.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(20838..21077)
                     /locus_tag="IWM23_001100"
     CDS             complement(20838..21077)
                     /locus_tag="IWM23_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883758.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            21363..21677
                     /locus_tag="IWM23_001101"
     CDS             21363..21677
                     /locus_tag="IWM23_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883759.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWM23_001102"
     tmRNA           complement(22111..22477)
                     /gene="ssrA"
                     /locus_tag="IWM23_001102"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(22648..23421)
                     /locus_tag="IWM23_001103"
     CDS             complement(22648..23421)
                     /locus_tag="IWM23_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9883760.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            23565..23915
                     /locus_tag="IWM23_001104"
     CDS             23565..23915
                     /locus_tag="IWM23_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9883761.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            23896..24612
                     /locus_tag="IWM23_001105"
     CDS             23896..24612
                     /locus_tag="IWM23_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9883762.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            24612..26060
                     /locus_tag="IWM23_001106"
     CDS             24612..26060
                     /locus_tag="IWM23_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9883763.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(26130..26639)
                     /locus_tag="IWM23_001107"
     CDS             complement(26130..26639)
                     /locus_tag="IWM23_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883764.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(26629..27354)
                     /locus_tag="IWM23_001108"
     CDS             complement(26629..27354)
                     /locus_tag="IWM23_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883765.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(27577..29697)
                     /locus_tag="IWM23_001109"
     CDS             complement(27577..29697)
                     /locus_tag="IWM23_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9883766.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            29927..31105
                     /locus_tag="IWM23_001110"
     CDS             29927..31105
                     /locus_tag="IWM23_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAP9883767.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            31121..31879
                     /gene="aroD"
                     /locus_tag="IWM23_001111"
     CDS             31121..31879
                     /gene="aroD"
                     /locus_tag="IWM23_001111"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAP9883768.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            31902..32387
                     /gene="ybaK"
                     /locus_tag="IWM23_001112"
     CDS             31902..32387
                     /gene="ybaK"
                     /locus_tag="IWM23_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAP9883769.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(32458..33711)
                     /locus_tag="IWM23_001113"
     CDS             complement(32458..33711)
                     /locus_tag="IWM23_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9883770.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            33829..35178
                     /gene="gdhA"
                     /locus_tag="IWM23_001114"
     CDS             33829..35178
                     /gene="gdhA"
                     /locus_tag="IWM23_001114"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAP9883771.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            35290..36636
                     /locus_tag="IWM23_001115"
     CDS             35290..36636
                     /locus_tag="IWM23_001115"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAP9883772.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(36944..37330)
                     /locus_tag="IWM23_001116"
     CDS             complement(36944..37330)
                     /locus_tag="IWM23_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9883773.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(37379..>37551)
                     /locus_tag="IWM23_001117"
     CDS             complement(37379..>37551)
                     /locus_tag="IWM23_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAP9883774.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT        12546 a         6489 c         7433 g        11083 t
ORIGIN      
        1 tcctagattg tagtaataat ggaaagggat agaagtaaaa cgagttgaat gaattttttg
       61 agggggagaa tattatgggc gactcagagg aaacccacat tcaacatacg tttgacagtt
      121 tttgtaagaa agtagtctgc aatgaggcgt taaatattca aaagaagtac gctagatttc
      181 gccaacggca aatatcaatg gaaatattgg aacaaaaagg gttaggtaca gagttctatt
      241 atttggaacc aaatttgaat agtagtggta cttttattgt ccttggtatg aaaatcttta
      301 tttccaatga ggagttggca aatgcgattg cagcattacc tagtcaaaga caagaaatca
      361 tttttatgtc cttcttcatc ggtcttaatg atcgtgaaat tggcgatatt attgggaaaa
      421 gtctcggcag tgtttggtac caacgacaaa tggctttgga agagctagga tttccatatt
      481 tcgattttgg ttggcatatt tttcatgcca aattctgtga ttttaatagc atcggcaaga
      541 tttaattttt cttcagctct ctctccactt ttaagttttt ttactgtact caagttgatt
      601 cctgttccga atgctattgc attagccgta gatttttcaa gaatttcttt aattattttt
      661 atgtctgcaa ttccttgttt catattaaaa attcctcttt tctagtccgt tttgatggag
      721 tactggagtg tttttttaca attttaaggg gtaccgtggt ggtcgttttg attttgagag
      781 agaagggtga tggagttgga agttaaaaca aatgttaatt ctctggaaat agagttctat
      841 tcaattttac aaaaaaaact ggaagattac acggaaaaat tgcttgcagt cagtcattta
      901 cctctggtat taacaaatac tgatttaaag cgactatttc agataagtga tagtacactg
      961 aaccgtttag taaaacttgg tgagtttcct ccctgttggt acgggattcg aggacactat
     1021 tttcgtgatg atgttttaga atggatgaga aagagtgatt cgaatgacta tcgagaaaaa
     1081 ttgcgactac ttcgatcttt ataagttaca ttttctttct aagatgcggt acaatgatga
     1141 catagataga aagatttcca aagggtttct gcttgttggg ttccttcaaa ataggaggaa
     1201 tcaataaatg gaaaaaacag atcgattcaa gaagcaaatc agttctactg gtaggatcac
     1261 atggtacttt caagcatatc gaacaacaag acgtggcttt aattcaaaac gtgaggcgca
     1321 actcgcttat ctggaaatgg agaaacaaca aaggcttaag aaaaaaattg ttgcaagcaa
     1381 tgacaagttt agtgttgtag gtgagcaatg gttccaatat taccgttcgt taaatgaaca
     1441 aaaggaaata acctatgata agcgagagga acaattaaaa attattgttc gatggattgg
     1501 tgataaaaag ttgtctgatt tgacaccaga gtgccttcaa gaattgcttt ttaccttaaa
     1561 ggaatgtggc gttaatggaa caaatgttgg ctatgccaaa aatagtttac aatcacttat
     1621 tcaagttcta aatatgattt ttaaatattg tcaaagaaag aatttgataa gtgaaaatcc
     1681 aatgaagtct gtgagaatgc caaaatatca gttaacggta actgatttaa aagaagccat
     1741 aaataatatt gaggacaagt ttcttactgt cgatgaattg agaacttttt tgaattatgg
     1801 cattgttcat gaggaattac cgatgtctgt acttttccat gttctatttt acactggctg
     1861 ccgagtcagc gaggcgcttg ctctacagcc tggagatata gatttcaaaa gaaatgaaat
     1921 tctgttctat aagcaaacgg cagttaaagg taagagtaag gattttcgaa ttgaaactac
     1981 taagacagtt agttctgcta gacgagttct agtaactcct ctagttatgg aaaagctaca
     2041 acagttgatt aaggctttgg gtgagatgag gcaacattac aggtttatta tagaagaaac
     2101 atatttgttt gtgtatcttg attctagtaa acgtggtgtt ccttatcgtc gagagtatgt
     2161 aaatgatcac gtcaaacggt gcgtgaatcg ctcaggaatt aataagccat ttcatactca
     2221 tttagctcgt catacaatgg caagcttagt agcggaacac tgtagctggg aagtcttgaa
     2281 agatcgctta ggccatactg accaaacaac ttcgaaaatt tatcgccact taacgtcaaa
     2341 tgaaaaggta aaacctctga ttgcttttag ttctttagag agttaaaaaa acggtgaaag
     2401 cactggtatt acaggattct tgaaaaacga ttttttaaca aattagtcaa tatgtagtca
     2461 gaggtaataa aaatcctttt atatcaatac tttaacgact tttcacaagt atcaaagaaa
     2521 tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa gggttctttt
     2581 ttcttagcat gtaatttgaa caccctgatc cagttttttc tttaattatt ggtaggggag
     2641 aataatggat tagaataaat ctacctataa gtagtagtaa aaaaaagagc actatgataa
     2701 tatctttctt aagagtaacc gctttaataa aaaatccaga atattctaac ataaatggag
     2761 tattcttaga atcaaaagga gaaagaagat ggtttatact tatacggagc aagaaaagat
     2821 gaaagtttta gacacattca tgaatgatgg aaaagtaaaa caagtaccaa gttcaacaaa
     2881 aaagaaatac atcctattga aagaagtctt gaaacgcttt gaccatggag tgacttatac
     2941 agaaacagag gttaattcga tactgctgaa cgtattttct agtggcgatt atgtcgaaca
     3001 aagacgttat ctaatcacat ttggcttttt caaaagaagt tcagacggtc gggcatatca
     3061 gatgatggga attgaaaatt agaagtttta atttatactc attgaaaaaa gttcaagtat
     3121 gcgatattgg ttctacaccg catacttgaa ctttttttgt catctgtatc tataatacaa
     3181 agccaagaac gttattcaaa atatggatag ctatgataaa agctaatgag cgattcgcag
     3241 ttaaataagc aatcgtcagt aaaatcgttc caggagcgta catgaaaaaa tcaattggat
     3301 gcgttacatg aataaagata attctctttt atttgtacga ataaatacca aaacgtacct
     3361 cctaaaatta gattttggac agatgccctt taaaaagata acaagatttc gttcttgaaa
     3421 caatatatat gcttttattc caatgaggtt gtgaaaacag tattttgtcc tgagcgttag
     3481 aaaattgaaa atggggaata gcaactcgta ctttaccatt ttttaagtga atacaagatc
     3541 agctaattga acaccgttta ttcgtgatga gggacacaac actgcagtcg acacatttct
     3601 cttttatgtt caaagataac aaatatttaa aacaaagaaa aatagtagat aatcccgtcc
     3661 taactctttt attcttccct catagcttcc aaatttaagc aatacttcaa ttaatcaagt
     3721 tttatctttt tttttcaata atcgtaaaaa aagagtattt tttatttaaa aataaaatct
     3781 ttgtattaaa ggacaagact aaaataacct tagaataaaa agagaaaaaa agccccaaac
     3841 tttctataca tcaataaaat aataaaaatt aaatttagaa taacgtgtaa aggattattt
     3901 gatgattttt tattgagagg agggatgaaa atgattaaga aaactcaaaa taaaatccaa
     3961 cgtatatgtc aaaaacattt tgtttctaga ctcaaagctg tatctaaaag aaaatactta
     4021 catcccatta tgatttcgat catcttgatt tttactttct tagggagctt atcaagtatt
     4081 tatgtaaacg tttatacaga aacgtcaaat tttgctaacc aaacgacaga ggagacaaat
     4141 agtccaccct attctgaaat acagtccata gaaagcacca atcagtctag caacaacgag
     4201 acaacctcaa ccacatcctc gactcaactg attgatccga cagaaaaaga aatcacagca
     4261 gcaaaagcag caggggagaa agcttattca gaaacaggta gaagccagac atttagtact
     4321 gttgcggcca ctgcgaattt caacggacat accataccag atggatatac agtgatatat
     4381 gccaaagatg gagtcattgt tgcggttaac ggatcgatga gtgcggataa aactgcgtta
     4441 caaggcgtaa ccaaagcaat cgcttttatt gataaccgtc cagatatcca taatctgttt
     4501 attggctcca cagtaaatgg tactactatc acattcccaa aaagtatcta tatgggggtg
     4561 gctaccgctg gaaatacgat cgattcagtt acacccaatg cggataaaag ttattctttt
     4621 acaccctcaa catggggagg gcttcctgcc ggaggaggta tgagtacttt ttcaatcggt
     4681 atacagttag caaatgatat tccattcacg gggtactcga tcaaaaatgg gcagatcgtt
     4741 gatggtaagg gaaatgtttc aaattttcca acctatcaag cagcaacggt tcaggtcaat
     4801 gcgaatcgga caggaacttt ttcttataca gtcaatggtg gagcagcaaa aatcggaacg
     4861 tacgggacta cattatctgg aatccatgtg ggtgacaaag tggttatcac tccaacagca
     4921 gtaacgggct attcttttgc acaaacaaac ggttctttca cagccctaga aagtaacgtc
     4981 gatacggtga cctatacagg caaacaaaca ccaatcacta ttaagtatca agactatttt
     5041 ggacagatga ttgctccttc taaaataatg aatcttactt acggaagtgc tgcacaagac
     5101 ctgacgacta atacgcctac tatcaacgga tataccttta cagctgtttc agcgatagaa
     5161 acaaagaacc aatcagcaat gagtgtttta gctagtttag atacaaatgg aaatgtaatc
     5221 gtaaaggacg caaatggaaa acagatttct gaagtcatcc tatattataa gacaaatgca
     5281 acagtatcaa tcaatgcaaa tggcagtaag tactatgatg gtctttctgt tgtaccgatc
     5341 gttaaatatg cctttaatga taaaacagaa tcaacatccc ttttaaacaa tgatctcagt
     5401 attgatcaaa tcacatggaa tccaactgat ttcaaagcga tgaatgaaaa gggagttgaa
     5461 gtaactgcgc ctactgaaat tggaacctat acgtcttggc aattgacaaa aagcggacta
     5521 gctaaactag ctgccagaac aaactattta tttactatcg tgcagacatc tgatgtctat
     5581 acgattaagc aaatcagcgg tgcggtagag ctcagtggca gtaagacata tgatggaaaa
     5641 gcaggtgtcc cgtcaatcca tgtcaagcta gcagagggtg tcactagttc gcttactcca
     5701 gtacaagtgg cacttagcag tatggattac acagcagatg cacaatcagc aaaaaatatg
     5761 gttaatgctg gcagctatac aattaaacta acgaatagcg gaatagacaa agtcaaagca
     5821 gcaaatccca agttgtcatt tactgatata gccaacacat caggaactta taccatctat
     5881 aaagctaacg cagtgattac tgtcgatgac gctgccttta attatgatgc ccaaagtcac
     5941 tcgatttcgg ttggaaatct ccacgttacc ggtgttgtct taggcgaatc attggattat
     6001 accctaacag ttaacagccg gacagatatt ggatcacaaa tagtaacgat tagtttaggc
     6061 gaaggaagtg taaacaataa ttatacgatt atcgcaacag ataccgctaa gttgacgatc
     6121 aatccgactc tcttgcttcc ttcgacaggt gggataggtc tactgccctt tgtcttttta
     6181 ggactgatct ttattttttc tggcttcttt tattttatcc atcgtaagaa aaaagcaggt
     6241 gaacagcgat gaaaaaaaca aaaaatgtac gtgtggatat atttgctgtt ctctttttta
     6301 tcctactggt cttttttata ggttctccga ctgtgtcagc tagagaatca cctattacat
     6361 ttactttgca taatcgaatg ttcgaaagcg ataagatgcc aagtatcagt caaaacgacg
     6421 gaataaatat cccggataac tttcaaggag taaaagggtt gaacggcgtt actttcgcta
     6481 tatatgatgt ctcggatgaa ttttacaagc tgcggtcaga aggaagtagt gtggaagacg
     6541 cacaaagaaa actagcacaa aaaagtgata gtgaaaaaat actggcagaa aatgtgacaa
     6601 aaacagtaga cgaggaagag ggcattgctt cttttagtgc atcggacaaa gatgagcaag
     6661 gtcgtgacgc tgtttaccga tttacggaaa taaagacttc agattaagta aaagaaaaat
     6721 cagcaccatt tgtcgttgtg cttcctgtga cagattcatc taatcataaa ttgacgacca
     6781 tccacttgta tccaaagagc gaacaaaaaa tacctgctgc actcacatta acaaaaacag
     6841 tagagaataa gcagacagat tttgcagatg gggataaagt cccttatctc atcaccgtaa
     6901 caattcctga gaacatcaac gagataggaa cctatacgat caaagataca gctgatcctc
     6961 agttatggct agaaccagaa acgatcgatt ttttgattgg tggagacaag attcatacat
     7021 ttcacacaca aaaaataaaa catggttttg tcttatcaga ttctggaaaa gatcttagtt
     7081 cgtttgctgg aaaaaaatta acgatccgtt accaaatgac attgaaagaa aacagtgaaa
     7141 aaacgacatt tgataacgat gtacgtttga cgactgacaa tcaaacagta gaaacacatc
     7201 ttgccatcca aacaggaggc aaacaatttg tcaaagtgga tcggcaaacg aaacaaaaac
     7261 tagcagatgc tcaatttcta gtcaaaaaaa atgaccaata cttagcgcaa gaaaacggga
     7321 taaatcattg ggtagccaaa gatgattcaa gagcaacgat ttttacttcc agtaaagatg
     7381 gaacattttc tgtccatgga ttgtcttttg gaagttacga actagtggag attaaaccgc
     7441 ctaaaggata tatattgagt caatcaacca ttctctttga agttgaaaaa ggcagttatg
     7501 atccgcttca tcctatacca ttggaaatca tcaacgaacc aaaagcaaat ccgacgaata
     7561 atggaaaaac gccagttcag atgcagacaa atggtccatc aaatgaaaaa aatggaggaa
     7621 gctttccaaa aacaagcgaa gagatgcttg gaggtttctc aattttaggg ttattgctag
     7681 tcctttcaac agggacagct tggttttata aaaaacaaca aaaaggaaac aataggaggg
     7741 aaataaaatg aaaaacatca aaacaaaaat atggacgttt ttaggaactg ctatcatgct
     7801 gcttcctttc gttctcggcc taggaactgc agaggtttct gcagccgtat cacctacacc
     7861 agaaaatgtt acggttaatt tgcataaatt gaaattcact tcagcaccag aaaatcaaat
     7921 aaacaacgga acggaattga catttccgaa tagcgaaccg ttgaacggcg ttgaattcaa
     7981 cgtatatgac atcactgcta cgtactatcc aagtaaagac acagcagttc cagcagacgc
     8041 tacacccttc gcaagtgtaa cgacttctgg tgaaggatta gctaacctga ctcttccagg
     8101 taaatctgat gggaaagatg ctgtatacgt ctttgtagaa acaccaaagc ccggtgtcga
     8161 aacgtcgcca aatatcgtgt taagcttacc ctagaccctg acaaaccttt agacacgatt
     8221 cacctatatg ctaaaaatgt catcaaagaa agcaatatca aaatccagaa agctgggaac
     8281 ttttcagggg tagataagtt agcaggggcg caattccaga tcaaaaattc agagaatcaa
     8341 ttcgtgaccg gttttgatga aaacactggt gtcgctctct atgatcctgc tgagtcagcg
     8401 ttgacattca caactgatac tgaaggatcc atcagtgtca atggattaat agacggcaat
     8461 tatactttag ttgaaacaaa agcaccagat ggttatcaat taaccaatcc acaaaaagaa
     8521 actactttta tagtaaaaaa cggaaaattg caacaaacaa ccggtatcat ccatggcgca
     8581 gatgaagcca atcttgggaa tccaaacggt gtagtcaatc gggaaaatac aactacggat
     8641 aacacgatta ctgtagaaaa tgtccaaaac tacggtgatt tccacttcat caaacaagat
     8701 gtgaacacgc aagaaaaatt agccgatgca gaattttcta ttctaagagc aaaagatcca
     8761 actgttaaat taatgaaaaa aatagcaaac cttgcctcag gagaataccc atatgcttgg
     8821 tcagatgaag tcacaggttc tgactgggaa gaagtgaaat tgatttctga ttcagaaggg
     8881 aaattttcaa tcgaagggct gaaacaaggg gcatactgcc ttcaagaaac aaaagcacca
     8941 gaaggctacg cattacctaa gaatgaagct gcgtacactg aatttacagt agatgatgat
     9001 ccaacaactt ctgatgaaac aacgatcgat aaccaaccta aaggaatctt gccttcaact
     9061 ggtggtatgg ggatcatcgt attcatcttg gtaggtactg ctttagttgg aggagcagtg
     9121 atctatttca aaaaacgcca tcaagaaaca gaagcttaaa ctattggaaa caaaaatcat
     9181 acgataaata aacgatagtg attcaaatgg ggagaaaggt tgtgacaagt ggtcggtctc
     9241 ttggtattcg ctcaacaaac ggcaaaatag gaggaactat tttgccgttt gcttcgctaa
     9301 tactcaggtc aaggcgctga tttcacaacc tcttcatata catattagaa aataggtgga
     9361 catgatgaat aaaagcagaa taagaatact ggacatcttc atggcaatca ttttagtcgt
     9421 tgggatcggg atcttttctt atccttttgt agaagattca ctgaatgatt ttcttgccca
     9481 gcaaatgatc atccattatc aaaagcaagc atcgaataag aattcagagg aaataaaaaa
     9541 acagcaggaa aaaatgacaa agaaaaacca acagttggca gagcaaaacg tatcaccagg
     9601 gataggcagt ttcaaccaag cagttgatgc aaaagcatta aaagatttac ctagtaatgc
     9661 cttttttatg gaacatatgc ttggagtcat cgaaatccca aaaatcaatg tcagcttacc
     9721 tatattcgat cagacaacgg aaatattttt gcaaaaagga acgtcattat tggaaggaag
     9781 ttcttatccg actggcggga aaaacaccca tgccgttctt tctgggcatc gtggactccc
     9841 agaagcaaaa ttatttacag atttgcctaa gttgaaaaaa ggagaccagt ttttcatcca
     9901 gatcaatgga aagacattgg cgtatcaagt agaaaaaatc caagtggtct tacctgacga
     9961 agtagattct ttgggtatcc aaaaaggcag agatctcgtg actttgctta cctgcactcc
    10021 ttatatggtc aatacacatc gactgcttgt gacggggcat cgtattcctt atcaagcaaa
    10081 agaggcaaaa aaagcgatcc aaggaattga tcaatggaaa aaatggaaat tttttatttg
    10141 gtttatcggt atactacttg gatcaatcgg cttagtttgg ctactcatcg cttatttaga
    10201 ttctttggcc atcgctaagc gaaactatcc tttatctttt tatgtaaaga acacaaacgg
    10261 acgaccaatc gaaggaatgg tcttttctgt aaaaacgctc aacggcaaac actatattac
    10321 tagagaaaaa gttccatttg tgaaagcttc tgatgaatac ggactagtga gattttctga
    10381 tttaaaaggc aggaactatc gcttgcaaca tgaggagctc ctgcttaaaa tccatgtaaa
    10441 acataaacat tcgaaacagt tttcaatgaa attgaaaaaa ggaagatata aactgcgaaa
    10501 agaaaaagag gtgtactatt tgattgagaa agaatgaatg aagatgtcca aaaaagcaga
    10561 gggaacagac taaatgaaaa aagagagaag ctgacgaaat ggacgtgtaa actgctattt
    10621 ttggtttact atctggaacg ccagcttcta tttttttatg tttcagaaga attttattat
    10681 tatagtataa cttttgaatt agtttccata acatttggag caaggggtta aaccacgagc
    10741 ttgagcttcg cttaaagtgg caggggtata cgtaccattt ccacatttat gtgtatggta
    10801 ttttgaaccg gtcggtgtga catagacagt ttgttcatta ttttgctcag gagctgcggc
    10861 ttgtgcctgc tgagaagcct gttcagctgc tgcttgctgt cgcgcctgtt ctgcttgttg
    10921 ctgtgcgatt gcttcttgcc tcgcctgttc tgcttgttgc tgtgcgattg cttcttgttg
    10981 cgcttgttct gcttgttgtc gtgcaatttc ttcttgcttt ttccgttctt cttctgcttc
    11041 ttttgctttg attgtggaat caattgcagc aagacggctt gtcaactctt gtttgttttc
    11101 aggtagcgct tggatagctg ctgaagcagt tgcatagttt tcttttgtag gatttgcttc
    11161 agcttgtgac aaagcagatt ccgcttctgt cacttttttt tctagtgcag ctttttccgc
    11221 tttttgtttc tcttcttctt ttttcttttc aatttgttct ttcgatgaat ctgccgtact
    11281 ttcggagatc gtggtactgc tgctggaagc ctgagctgaa cttgtgcttt gagtagtaga
    11341 cgtagtagca ccacacccac tcaacaaact tcctgcgacc aaaaacaacg caactaattt
    11401 tttcactaaa tttcctcctt aagtaaaaaa caaccgtttt caataaatgt ccttttcaat
    11461 tgtttactta cttgttataa taattgtgtt atgaatagtg attgaggaaa cttaatctaa
    11521 tcccgtgttg atacacggga ttttttgcta tagttctgtt attaagtatg caataataaa
    11581 atatgtacgt tattttatta ttagattatt gacatttata taataaacga ttaaatgatt
    11641 catatcaata gaatacttag gaaaaaaaga gaaataataa caggtttatg cactattgac
    11701 ggtgtgacaa tatgatattt gtatccgttt ttttcttgta tgtagtatag taaagtagat
    11761 acaggaggag aaaatatgca gactttttta acaaaagttt ctcaaaagaa aattaaaatg
    11821 attcatttac tgcttgaaac agaacgttgg tgttcaatag aagaactaca aaacgaattg
    11881 aaggtatcaa gtaagtctat cttgaattat ctaacagatt tagaagaatt gttccaacaa
    11941 tatcctgata aggtcatttt gaagaatgag aataatcggc gcttttctat ggaaaagcag
    12001 gagaacttcc caatctacat tatttatctt catttctaca gaaaatcata taattatcat
    12061 ttgattgaat tcatgtatca acatcctgag aaaaatttgg aagattatgc agacgctcaa
    12121 tatacgagtg tatccacagt ttttcgttat gctaagttat tagtaaagta ctttgaaatg
    12181 acggacgtca aagaatagtc gccgccagat agaaggaaat tagcagttga aaaagctgcc
    12241 ctgcatcaag taatacgacc agccaaacgg aaacagtgtc tcatgttttt gatttggctg
    12301 gtcttattac cgcagatgca agctttttcc acacctcttt cttgtcattt acagactcaa
    12361 ctatcaatgc tgcaagcttt cccttaatgc tgtaacacca gtaagagttg tcaagaccag
    12421 cagactgact aggtaaatac cgctcaaaaa caagattacg gcagttaaac tataatgatc
    12481 catcaagaaa ccgatgacga tagaagaaaa accaccaaca gctcgtccaa ggttcataac
    12541 gaaattattc gccgtcgcgc ggatttctgt tggatacaaa ctactaatca ctgcgccata
    12601 tccaccatac atgccattga taaagtagcc gactatcaca gcggctgcta ccaaactcca
    12661 ctggctatgg gctaacacaa gtagatagac agataaggat gaacaaatta aaaacaaaga
    12721 aaaggctagt ttaggcccca atttatccat gattatgcca aaagtcatca tccccaaaga
    12781 catgcctaaa atagtactga ccatccacaa tgaagaccca gaaaccgaaa gccctaatcg
    12841 attttgcata attgaaggta gccaattcat taacccaaaa tagccggcaa tttggacagt
    12901 taccatgata ctaaggccac atgtttgcca agcaagatgt tttgattcaa acagtctgct
    12961 aagtttggtt ttcttttcgg cagattgtac agatttgaat tcctttggtt cagtcaagcc
    13021 gcgacgaatg aacaagacca tcaacaccgg cagtacacct acgacataca acatcttcca
    13081 accaaacgtt ggaatgatca cagcagctaa aatcgcagcc aaagccgcac cgacctgcgc
    13141 accaatacca gcaaccgaag aagctcgtcc aatttgtttt tttgaaaaac tttcggaaat
    13201 caaggacata cacgccccat attctcctcc cgctcctaca cctgcgataa agcgaaagag
    13261 ataaaccaag tagatgtttg aagcaaaata catcaataaa gaagcaatcg aaaacagaag
    13321 gaccgtttga gaaaatactt tcacacgtcc atatttatca gctatgatgc caaaaaaaat
    13381 acctccaatg agcatcccca aattagtgat cgttgaaatg aaacccgctt gcgtaccact
    13441 aatatgaaaa gaagtaatca ttgaagataa tgaaaaagct aaaaacataa tatccatatt
    13501 ctctaaagca attcccgccg atgtcgatac taataccttt cgctgatacg ttgtcatttt
    13561 tatacccctt ttcctaaaat ttccctaaat tgttaaaagt atatccaaaa aagaataaaa
    13621 aaacaagact tttttatttg aaataataca caaacaaccg atatagataa tccagcttag
    13681 ttttaataga aatgagtatt gacttggagt acacttaaag ttgtagaata aataaaatac
    13741 ataagtaaag tggcaagaaa ttaagagaaa gagtgatcag atgaatatca aacaagtcag
    13801 tgaagaaaag ggcatttcgg ccgatacatt gcgctattat gaacgaatcg ggttgattcc
    13861 accagttaat cggacaaaag gcggaatccg tgattataca gaagaagacc taaaatgggt
    13921 cgactttacg atctgtatgc gaaatgccgg attatcgatc gagtctttga ttgaatatat
    13981 tcgcctatct agcgaaggag aagcaactgt atttgagcgg cgtaagcttt tgattgaaga
    14041 aagtgaactt ttgacgaaaa aaattgctga aatgcaagaa tgtttggaac gtttacaagg
    14101 aaaaatcaag aaatatgatc gtgtgttatc tagaaataaa atggaatgta tcaagtaaaa
    14161 aaatcaagga gcatcgctat ggagttacgc ttactaaaat atttttggac aattgcagaa
    14221 gaaggaacaa tctctcaagc cgcagaagtt ctacatctta cacaaccaac cttaagccga
    14281 caactgaagg agttagaaga gtaactagga acagaactgt ttgtacgcga aaaccgcaaa
    14341 atgatcttga cagaagcagg ttattttcta aaaaagccga gcagaagaaa ttttagattt
    14401 aacgaatcaa accactcaag aattcgagaa cagaaaaaaa caattattca gcggccgatt
    14461 tacgatcggc tgtgtggaag ctgatagttc gaatacattg gcactattac tagaaaagtt
    14521 gatccgtgat tatcccagcg ttcaatttca tatccacagc ggaacaagtg atgatatcac
    14581 ggagtgacta gaaaaaagcc tgttagattt agcgattttg ctagatccag tagatacaga
    14641 gaaatacgaa acgattttgt taccaagaaa ggaaaaatgg ggattgctca ttttagaaaa
    14701 ttctgtttta gcaaaaaaag acgtgataac aaaagaagac tttgcgaatg tacctctttt
    14761 gatctcaaag cgtccagaaa tacagcgaat gtttgctgac tggttaggca taaaaaagga
    14821 gcagttgctg atcacaggaa cgtacaattt gattttcaat gtatttgcgc taattgagaa
    14881 tcaggtaggt gctgctttag tcatagaagg tgctgtaagt catcattcaa agcgagtgac
    14941 attcgttccg cttgacccaa tgattgaaac gaactgcatg ctagtctgga aaaagaatcg
    15001 gattttatca cctgtagtca atgaattgat tcaccgtttc aaagaagctt ttgaacagta
    15061 ataagtatct ctaatcgaag aaatttcaat acaaaagcga gacgtatgac agtcttgctt
    15121 ttgtattttt tcatttttaa atcaaaaaaa taaagagatt tacataactt gttaaataag
    15181 cgcttacaaa aataagaaga acggaaatga atcagctata ataaccgttg ttctggattg
    15241 taatcattac aaagaaatga agattataac tttggaggtg ttcgaaatga aagaaaaaaa
    15301 gcaaaaattc gttcaaaaaa catgcccttt attcaatggt tgggtatgat tctgctgatt
    15361 gtattagctt tattgacgat cattcctttt ttatggatgg tttctgcttc attcaaagca
    15421 aataatgaag tgttcacgat tccgattcaa tggatcccta aaacttggca tttagagaat
    15481 tatctggtta tttggtctcg tattccttta gtgacatttt ttaagaatac agctttttta
    15541 agcgttgtga tcacgttgat tcagttgttt acttctagtt ttgctgctta cgggttttct
    15601 aaactaaatt ttaaagggcg cgatatttta tttttagcat atatcggcac gattgctgtc
    15661 ccttggcagt cttatatgat cccgcagttc attatgatga gaaaagtggg tttaacagat
    15721 acgttgtggt cgttggtgct attacaagcg ttcaatgctt ttggtgtgtt cttgttgaag
    15781 caatattaca gtagtatccc agattcttta tgtgagagtg cgcggatcga tggcttatca
    15841 gagtggggaa tctatcgcaa gatcatttta cctcttacaa aaccggcgat tgccagtttg
    15901 acgatcatca cattcgttaa tacatggaac gattatatgg ggccattcat ttatttgtct
    15961 tctacggaaa ataaaacaat ccagttaggg ttgaaaatgt ttattgggct atttgatgca
    16021 gagtacgcat taatcatggc tgcctcagtt gtttctgttc ttcctgttgc ttttatattt
    16081 ttagcgatgc aaaaatattt tgtagaaggg atcgcttcat caggaatgaa ggaataggag
    16141 gtgattatga aagagcagcg ttttgacaaa aatgtttata tggaattctt ttattggttg
    16201 tatcaatcat tggcaagtag cctttgtttt tggtgtgtca atatcttgta tatagcagct
    16261 gcgttccttt tgtcgataga tggaaggaat gtcttttcct ttattttttc tttacttttt
    16321 attggtccag gttgcatcgt gatgataggt atgacagaag aattagcaaa agaaaatagt
    16381 ttttcaccag tacgtttgtt cataaagatg tttcgccaat tttggctaaa gggatttttt
    16441 tattggctat ttgcttggat agttagtgtg ataatgattt ttgattgttt cttttttata
    16501 cgtttttctt atggaaaatg gttgattccg ttgtttgttt tacttgcttg tttatcagtt
    16561 tcctttagca ccaactgctg gtattttcag gtgagaaatc cagcgtcgaa accaaatcaa
    16621 gttctacgga tagcgttcta ctatacgttg aaaaaatggt atgtgagttt actagatttt
    16681 ttactattga caagtttgtt tctcttcttt ttcgtaaagc cccaatggtg tattctacta
    16741 ggtccttcta tcgtatttgg tttgatttac ttcaacaatc gtaaattgat gagaactatg
    16801 gatctgtaga ctttataaat tgaaactttc aattaagaat gacagttttt tttatgtaaa
    16861 aactgtcatt ctttttcatt ttttgaaaca tatggctaaa caaatgaaat aaaacgtgct
    16921 atacttttgt atgaaacagg tttagaaact gtatcactta tttgtgatta tttctaacta
    16981 ataattaaaa ataaagcata aaaaggacga tgttcaatgg ctgaaaaaat gaaacaaggt
    17041 tcgaaatctg tagtagaaag tttgattaat catcatgttg attatgtatt cggtatacca
    17101 ggtgcaaaaa tcgatggtgt atttaatgaa ttagaggatc aaggtcctga attaatcgtg
    17161 actcgtcacg aacaaaatgc tgcatttatg gcacaagcaa tcggacgtat tacaggagag
    17221 ccaggagtag ttatcgcaac aagtggtcct ggtgctagta acttagctac aggtttggta
    17281 acagcgacag cagaaggtga cccagtttta gcaattgccg gacaagtaaa acgaagcgat
    17341 ctattgaagt tgacccatca aagcatggat aatgcagcac tgttccagcc aatcacaaaa
    17401 tacagtgcag aaatccaaga tccagaaaca atttcagaag tgattgccaa tgcttatcgt
    17461 atggctaaga gttcaaaaaa aggcgcgagc tttatcagta tcccgcaaga tgtagtggat
    17521 gcacctgtcc aaggtaatgt catcaagcca ttgagcgatc ctaaattagg ttctgcttct
    17581 gcagatgata tccgttacct agctgaacgc attagagaag caaaactacc ggtactatta
    17641 gtaggtatgc gaggatcaag tgaaaaagaa acgttagcca tcaggcaatt agttgaaaag
    17701 acagcattgc ctgttgtaga gacattccaa gcagctggag tgatctcacg tgaattagaa
    17761 gcgcatttct ttggtcgtgt gggtttattt agaaaccaac caggagatat gcttttgaaa
    17821 cgaagtgatc tagttattgc aattggctat gatccaatcg aatatgaagc aagaaactgg
    17881 aatgcggaaa aagatgcgcg gatcattgtg atcgatgaag cgccagctga aatcgatcct
    17941 tttatgcagc ctgaaagaga attgatcggt gatatctcag ccactcttga tttattgaca
    18001 ggttcattag agcctcaaca agtgtcagaa gatgcaaaag aatatttagc atctcttcaa
    18061 gcaaaattaa cggagcggga cattgttcaa tcaaaaggag aagcaggtat cttgcatcct
    18121 ttagaagtta ttaacacgct tcagtcaaaa gtaacagatg atatgactgt taccgtagat
    18181 gtaggtagtc attatatttg gatggcacgc cactttagaa gttatgaacc tcgtcatttg
    18241 ttattcagta atggtatgca aacgcttggt gttgcattgc catgggcgat ttctgctgca
    18301 ttggtgcgtc caaatacaca aatcgtatca gtttccggcg atgggggatt tttattctct
    18361 gcccaggatt tagaaacggc ggtacgtaaa aaattgaata tcgttcattt gatttggaat
    18421 gatggacact acaatatggt agagttccaa gaaaaaatga aatatcagcg ggcgtcaggt
    18481 gtagactttg gtccagttga ttttgtgaaa tatgcagaag cttttggcgc taaaggaatc
    18541 cgagctacaa gtgtagagga attagaaaaa gctttagaag aaggatttgc gacagaagga
    18601 cccgtaatta ttgatattcc gattgattac cgtgataatg agaaattggg agaaacaatc
    18661 ttaccagatc aattctatta aggaagtgtc gacatgactg aaaaaatctt ataccagcac
    18721 gggacattag gagctttgat ggccggcttg atggatggaa cagaaacgat tgctcatata
    18781 ttagaaaaag gaactctggg attgggaaca ttgcatggtc ttgatggtga agtcatcttt
    18841 ttagatggcg tagcttatca aggaagatca gatggctcag ttgcccaatt agatggaagt
    18901 gaacttaccc cctatgcagc aattacagat tttacgccag atactagttt ttctgtctca
    18961 aaaactgcag atggagaaca attgaaaaaa gacatattag taagagaagc cggggaaaac
    19021 ctatttttgg cagtgaagat aactggatta ttcaaaaata tgcacatccg tatcatgccg
    19081 aagcaagaaa aaccttacag aagactagca aaaatttcag agtctcaacc agaattccaa
    19141 caaagcaata tccaaggaac attagtcggg ttctttacgc ctgaattatt ccaaggagtt
    19201 gctgcagcgg gatttcacct gcattttatc gatgatacac atacatttgg cggacatgta
    19261 atggattttg aagtggctga aggaaaagtt gagattagcc gcatctcgtc ccttgtacag
    19321 cattttccag ttgatgatcc gacttttgtt cacgaaacga ttgattatgc tgatttagca
    19381 gcagaaatcc aacaagcaga atagaaaaag acagcgagat gcgttcaagg tgattaacct
    19441 gacgtatctc gtttttttga ggaggaaacc tagcgtttac aaaaaaactg agctataatc
    19501 ttctccatag atcaaaaagg agcggttttt atcatgtgga cagaaaaaca gaaaaaagat
    19561 gcatacgaaa ttttattatt acagcaacaa ggattattag aagcagaaga taactggtta
    19621 gcaaatttag caaattcatc tgcattgttg aatgagactt tgcctgagac tgtctttgca
    19681 ggatattatt tattcgatgg taatgaattg attttaggac cgttccaagg gcgagtttct
    19741 tgcacacgta tcaagatggg aaaaggagtt tgtggagaat cagctgaaaa acaagaaaca
    19801 ttgatcgttg atgatgtcaa aacccatgca aattacattt cctgcgattc agccgcacaa
    19861 tcagaaatcg tagtgccaat ggtaaaagaa ggtaatttaa tcggtgtttt agatatcgat
    19921 tgcggagtaa cgaaagggta tgatgaaatc gatcaagcat ttctagaaaa atatgtagat
    19981 cttttattag caaattttaa tataaatgct ttttcattta ttaataacta aaatcgtcta
    20041 ttgaaacaaa aaagaagaga aatcaagaat aaaaagagcc ggaaagacag tggtcatttc
    20101 tgtttttcgg tttttttatt tttttataaa atcacaagtg ttattgattt tcacaatcat
    20161 attttaatgg tattatcatt ttgaataatg aaaaaacatt aaggatggat gaaatatgaa
    20221 taacgaaact atcattttag gaaaaaaata tatttgccaa cctattggct tgaaacaaaa
    20281 agtggttgga gaagtaatca gtaaattaga aaattgtgtt gtgatctgcg tagacgaata
    20341 tcatgacgtc gatcgcaatg aaatattaga aaaatgcgga aaagtagtgg caaaatatac
    20401 tcacgtttat gagttggaaa caacagaata tctatataac tatagcaaca aatatgaaca
    20461 tactttagtt tcataaagat ttaaataaag tgccttcaaa agcgagaaga ccaagctttt
    20521 gaaggcactt tatttagttg gcagtgattg ttcatgattt ttgatttatt caaaaggttc
    20581 gcctagagaa tctgtctctg taacgaggcc tggtggagaa actcgataaa aacctatact
    20641 gccagaactg tcctattttc ggatagacaa actagtagtt ttaaaaagat catccgatcc
    20701 aatcatttca aaaaatgcaa aactgatatc aggatcattt agctcaggct tgttatcgta
    20761 aagcatttgt aaaggttgca tttagttagg agtaaacgcg aaactatcat cattatttgc
    20821 tgaagaagat gaagaagcta attcagagtt tgtttgaaat aaaggagacg tgctgacagt
    20881 ttctttaccc tgttttttgc agaggtagaa acttttatat cggaagtttc taccggagaa
    20941 tttttctcat ttgtagaaat tgttgaatcg atactattcg aaatttgcgt atctgaaggt
    21001 atgttctttg atgcagaagt cccacaagca gtaagtaaca ataaatttga taaactgata
    21061 aacagaattc ttttcattat gtacacactc cttatagtat cgtttagcat acacctatgc
    21121 tcagtattag gcaaaatcaa tgcaaaacta tcaagtcttt tatagtatct attagtctta
    21181 gtaatacata tatggagagg gtgatatccc agttcaaacc agatacattg gttaccatct
    21241 gtattaacga ttatgcctct aaacgctttt tttatgaaaa aatttataag tgaaaacgta
    21301 ggtatgatat tatctagagg taattttttt gggtatttaa atagttgcta ggaggatagc
    21361 ttttggattt ttacagtaat tttatcttaa taatagcaat attattactt ctaaatattt
    21421 ggttttttga taagagtaga aatgcaggga taggttttag aaccaaacga agtacgtctt
    21481 ctgaaaaaaa gtgggtatat tcacagacta ttttttacgg aggggttatt tcaatcagtt
    21541 tactttcttc aacactttat tcttttaatg ttatagacgt ttccatgtca aattttatat
    21601 ctattattgg aatccttatt tctgcaatta ttacacaact acttcttgtg tttgaagaga
    21661 agtcaaaaaa taattaaagc aattatgcct tttagtacag gaattattac ttcttacaaa
    21721 tgtatagcga atgtgaagcc tgctgtttta attgttctag aaaaactttt gagtgaacaa
    21781 ttattttatg ttaggatgaa ttaatttctc tagagtttat aattaaaaaa cttatttgaa
    21841 ggaacatgct taaaccataa ttgtgttttt ccttccatca gcataaaagt tcacctcaaa
    21901 aagagtgtac gaacaaaagt tctttttgta aaccttaaaa tagattttcc tgaatacgta
    21961 ttggggattt ctaaatgttt gaatgttttt ttgaatgcaa aatcaatgca aaaaattttg
    22021 tatttctcta cttttctctc cttgaccaaa ataaaaaaac gttgatttaa caacgttttt
    22081 catacatgtt tatctgtctc aaccatacat atatggagac ggcgggagtc gaacccgcgt
    22141 ccaaacacat cgccacttaa aaatctacgt tcatagtcaa ctcatttaag gattcacttt
    22201 acagcttgcc gagtgacagg cattctgtat cgctagtctg atgttctctt tttcaactta
    22261 cagacggaaa gttgaaacgt atcccactaa agttaggacc cttactcgag cacatgggcg
    22321 atgccgagag gatctacgct aactgttttt aggcagctaa agcgtaagag ttgttttcgt
    22381 ttttagcagt tatatttaac tgtaacgttt ttacgtagac gtaacctacg aaacgcaatt
    22441 caagctcgac tgtgcctgtc gaatccgtaa cgtccccgtt agctaacaag ggttacgtgt
    22501 atttttcata caaaattagt atagcatata ttgggataaa ttcaataaaa aagcgaatca
    22561 tatcccatta tttatataat aactgcccct ttcttaagaa aaaataagcg attttcttaa
    22621 gaaaagaggt agcttcaaaa aaagatctta aatcagatca aaatacttca atgcgtggac
    22681 aattccgcct tctgtatttt tctttgtgat aaaggtagaa agttctttta atccgtcata
    22741 ggcgttgccc atagcgattt tgttgtcgca agcttccaac aaggcaaaat cattcggacc
    22801 atcgccaaat ccaaaagtag gaactttttc cagctgaagt tctttttgca gacgtttgac
    22861 acctgcccct tttgagattc ctttcttcac tgtatcgatc gagtaaggac cattccgata
    22921 aaaagttagc tcaggaaaat gttcgtgata gtattcgtca cctgattgag ataagatcag
    22981 caacatattt actgtgtttt ctttaaagcc taaaggatca atttccggaa catctgaatg
    23041 aatgaagtcg taagcattga tcattgtttg gctatgaccg gtacaccaga ttttctgatc
    23101 gttatagtaa gaaagttcat gaccgttcaa ctgtacatga tcatacattt tttgacattc
    23161 ttctgaagaa aatgtttgag aatagatttc ttcgccgtct actcgaatga aagaaccatt
    23221 catggtgacc gcagaagtaa tgcccgcatc ttccatgata gagtggattt ctgcttctgt
    23281 acgtcctgta gcgatgacgg ggagaacatt gttttctttt aatgcgttca tagcttttgc
    23341 cacttcgggt gtgatttttg atttcgcatc tagtaatgtt ccatcaagat caaagaaagt
    23401 gattgcacga taatttgaca caaataaaac tcctttttta ttggaataag tttatcttaa
    23461 caaagaatga acaaaagaca aacctttcgt gaagaattcc tgaacatatt ggttcagtca
    23521 acaatttaga gtatactata agaagagaat ttaggaggga aagaatgatt tttttcagta
    23581 ttggtatctt attcttaata attggcagtc tttttcggat tctgccctca aaaggaaatc
    23641 tgccttttta tgggtatcat tcgcctcttg ccgcaaaaac agacgctcat tggcggctgg
    23701 ctcaaaagac aagcggaaac tggttttttc ttatgggctt actgatggca ttgatcggct
    23761 attatttaaa gacatctggg catacgaatt attttctgat tgaaatgctt ctgctagtat
    23821 ttccaattat gcctattttt attatgacag aaaagaaatt acagaaatat gacctggaaa
    23881 ctggagggaa cgataatgaa tattttaatg attgaagata atcaatctgt ttctgaaatg
    23941 atgcagatgt ttttcttaaa tgaaggttgg gaagcaactt tcaagtatga tggaaaagaa
    24001 gggctcgatg cctttttaga aaacccacaa cattgggata tgattacact agacctgaat
    24061 ttgcccacac tggacggtat gctagtagga agagagatcc gcaaagtttc aagtactgtg
    24121 ccgattatca tgctgacagc tagagattct gaaagcgatc aggtgatcgg cttagagatg
    24181 ggggcagatg attatgtcac aaaacctttc agtcctctga cgcttattgc tcgaatgaaa
    24241 gctttgcatc gccgtagtga gttggcagaa gcaagagaag aagcagcagt ttcagatgat
    24301 cattttgata tcgagactag tcatttcaag atgaatacga aaacacgaga agcgtatctg
    24361 gacaaccggc caattgaagg actgacgcct aaagagttcg atctattgtt tactttagca
    24421 aaaaaaccac gtcaagtatt ctctcgggag cagttgcttg aattagtctg ggattatcag
    24481 tactttggtg atgaacggac agtggacgcc catatcaaga aattacgaca aaaaatcgaa
    24541 aaggtcggac cacaagtcat ccaaactgtt tggggagtcg gctacaaatt cgatgattct
    24601 ggtgttgcct aatgcgttat ttgtatcagc aactcttagc tttttgcgga atggtcgcgt
    24661 tgatcttatt gatcgttgga gtttctttta cacaactgac aaaacaaacg attgaagaaa
    24721 acaattacca gcaactgttt ggatatgctg aatcagttga aaagacaacg caaacctatg
    24781 ccgatacgct cccgcagttt agtcaagatc aggcgtttca aaatgcattg ctgctgacag
    24841 agcaagtact gacagaacaa aatgtgaact ttatatttat cgataaaaac gaacgtgtca
    24901 tttatccgac taatacagct ttactgaatt ttaccttaac agctgatcaa tggaaaaatc
    24961 tgaggaatgg acgtcaagaa aaattcacat cgaataaaaa tattttagga caagcacagg
    25021 cgacctctta cgcacttgtg ccatttaatt tgaatcatga attctatgga gcacttgtag
    25081 ttagtcaacc tgcgcgaaat atcgataaca gcgttcgtcc aattacactg aacctgttca
    25141 aaggatttat tttttccagt atcgttgcag ttattgccag ctatgctttt gcagcattcc
    25201 aagttaagcg tatcaatcgg ttaaggaatg cgacgaaaga agtgacgaat ggtaattttg
    25261 atgtacagct ccccgtacat gacaaagatg aatttgatga gttagcagat gatttcaata
    25321 aaatgacgaa ttcattgaaa gaatctcaag cagaaattga agaacaagaa aatcgtcgaa
    25381 gacaatttat ggcagatgct tctcacgaga tgagaacacc tcttacgaca atcaacggac
    25441 ttctggaagg gttagaatac aatgcaatcc ctgaaaatca gcgagaaaac gcgattaaat
    25501 tgatgaaaaa tgaaacagag cgtctgatcc gtttggtgaa tgaaaacttg gattatgaaa
    25561 aaatccgtac gaatcaaatc agtatggtca ttaaaaaatt caatggaaca gaaacattga
    25621 aaaatattgt tgcacagtta gaagctaaag cagaagcagc aggagataca ttgacattga
    25681 aagccgttga tgatattgat gtttacgctg attatgaccg atttgtccaa atcatggtca
    25741 atattattca aaacgcgata cagtttactg aaaacggcca aatcacgatt actttagaaa
    25801 aaggctatct tgaaacgatc atcacgattg aagatacggg aattggaatg tctgaacaac
    25861 aaatgaaaag tatctgggat cgttattata aagtagatcc ttcaagaaaa aatacaaaat
    25921 atggtgaatc aggattaggt ttaccaatcg tgcagcaact tgtacgcttg cataaaggaa
    25981 aattagaagt agaaagcgaa ctaggaaaag gaacaaaatt cgctgttcgt ttgcctgacg
    26041 tagaaatcaa aaaagagtaa aaaacaagcg gtataaaaga tcatgaatcg atcttctata
    26101 ccgcttgttt ttactataaa atgattgatt tagcccgcgg gctctaaacg atcaatataa
    26161 ggactttgag caatagtagc atcatgagta aagagtaaga taccttgatt ttcttcatga
    26221 gctaaataag aaagcaagtg gagtagctct tggatctcca atacactaag ttcatcaaat
    26281 gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg catgaagtaa ttgcagcttt
    26341 actttttcaa aagacgtcaa ctcagctgcc gcacaattaa gcaaggaagg agagatatca
    26401 agtttttctt gataggccaa acgatcttgt ttgatttctt tttccggtac atccaacagg
    26461 acatggtctt taagattcag ataaggaaac atcggccaat ttttttggat gatcgcaaaa
    26521 ttatccagtt ttgcaacaag aacctctttt ttcaagtgct ccaatatttt atgctgtttt
    26581 tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa tcaaatattc aggtttcatt
    26641 acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa gtgaaaggga atagactaag
    26701 aatgagacag catccactga gcccaataag agtttgggca aatcccttaa gtatatctgt
    26761 gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc tgttgatcca tcggaattgt
    26821 aagtccgctt accgaagtca agatatcacg gatatctggc agttcagtag tctttaagac
    26881 aagaaaattg atcctttgaa gaagcgcttc ataaactgac tggaagctta cgatccacaa
    26941 taaaaccaag atacaacaaa gaaagagagg aaagaacagc tgccagaaaa taaaaaggaa
    27001 taatttggtt ttagaaagac cagtcaggcg ccaattgatc aattcctgct tgttttgcct
    27061 gactgaatag taaaagaaaa agataaaaag gaaggcaaat agaaaaaata aactgaatat
    27121 gccttttata taaaatcggc tagctaattc taaagccgga agaagctctg tagatgcaga
    27181 agcgccaata aatgaaagcc gatcttggac ttgattggct aaagccgatt gcaaatggta
    27241 aagagtcagg catgctatca ataaaaaagc agaaaagctt gaaaataaac aatagagtat
    27301 tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag cggattattt tcatactttt
    27361 cacctctata aaaagtatag aaaatgaata tgaataaagt atgaaattcc tttagtgaaa
    27421 tcacgaatta aaaccagaaa atattttgta aaaagcaaga ggttgtgaag aagctgccct
    27481 attctattac cgtagatgca agcttttgaa cactgtttat tcgaaaagag gagcagcgac
    27541 aataattttg tcgctgctcc ttttaaattt attttgtcat tcggaagaac ttgtggtcgt
    27601 tccgtgaagt tcttggtacg tttgcgtttt ataaagatca acagttgatt gtccgttgtg
    27661 ttcactaaag acactagttg acttgtcacc aagagatttt tcagcttctt ttaatttctc
    27721 taagccattc ttgtagtcat aatcactggt atcaattggg tctaatccac tattcgtata
    27781 gaaccgaaga agatcaccat tattgatttg gtcagaaaca gctaactgtt tatcaacttt
    27841 ttctttcagt gaatcaattt cattctgtac ttcctcagta ggttcagtaa tagcttcacc
    27901 agtttcgtta ctatagatcg taccaccgta gtaagtatat tttggtgtaa caaaatcacc
    27961 atcacggaaa gaaacaagct gttcatgatc tttcgagaat agatcttgtc ctaactgaat
    28021 ataatttttt gtatctacac ctagtaagtg aagtaatgta ggcaaggcat caacttgtcc
    28081 gccgtaagtg tgattgattc cgccctgtgt ttgtccaggg atatggatca tataagggac
    28141 acgctgcatt tgtgcattgt cgtagtttgt ccaagtagaa cttgtttttc caactaattc
    28201 agctagattt ttatttcttg aattcgagac accgtaatgg tcaccgtaaa ggacgatgac
    28261 tgaattatca tacaagccgc tttctttcaa ataattgaag aattcttgta cggcggtatc
    28321 taaataattg gctgtcgcaa agtaaccgtt gatcgtttca tcatttgtat tggctaacgg
    28381 gaatcctgct tcgtcattgg tgaattttga ataaggataa tggttagata cagcaatgaa
    28441 ttttgaataa aatggttgtt gcagacgttc caagtattgt acggattgtt ggaaaaatgg
    28501 tttatcatgc aacccgtatt ggaacgaatt gtcatcatta acatcataat aagaagaatc
    28561 aaagaaataa tcatatccta aattcttgta tgtctcgttt cgattccaga atgtaccagc
    28621 gttcccatgg aataccgcac tagtgtaacc ttgtgtttga cttaaaatat caggtgcagc
    28681 ttggaaagta ttttttccgc ctaattgtgt gaacaaggag ccttgattca agccaaacaa
    28741 tgaattttcc atcagcgttt ccgcatcgct tgtttttcct gcttttactt gatggaaaaa
    28801 gttgtcaaaa ctgaacgtgc tgttgctgtg gtaaagacta ttgatgaaag gtgtaacttc
    28861 atgctggaga ccattctcat ctgtcaattt ataatcgatc aggaattgct ggaagctttc
    28921 taaatggata taaatcacat tttttccttt agcgagcccg aacaaatcgt catttggtgc
    28981 tgcataatgt tcttttacat aattttctac ttctttcata tcattgggac ttgcttctgc
    29041 ccgcacttgg tttgtctggt aagtttgaac accgtcataa accatgaaag cattcaagcc
    29101 aagatatttg accaaataat cacgagagaa tgttcgagtc agcaattctg gacgatcagc
    29161 ttccgccata aaaagatttc ctgagaaaat cataactgct aatgtagaga tcgcaaaagc
    29221 cattcttgct cggatcgggc gagggtccaa cttgattttt tttgcaatca gcagaacagt
    29281 caagataata aaatcgatcc aatagatcac atcatatggg cgaaacagtc gaatggcact
    29341 ttctcccaga ccactcgcta ctttcccagc acctaaaatc gtgttgatcg taatgaagtc
    29401 cgtgaattct cgataataag tgacattcga aaaaacaagc aatgtcaaaa gaaaataaat
    29461 gaccatcatc gtgatataag caactctagg gcgtctgata taaagagcaa ttccaagtaa
    29521 taacaatgta gttgcaatag ggttgataaa caagataaag tattgaatgc cactttcgat
    29581 tccgagatgg aaatcaacta aataggcaaa aatcgttttc aaccaaagta aaacgactag
    29641 taaagaaaaa aatccgagcc tcttattcaa aaaggcaggt gtttttatac atttcaagat
    29701 aatcttcctt tctgcacttc ttaatcacag aagtgtcatg aaaccatgtt acacagctca
    29761 cttattctca tttattttac tagtatgaca atatagtgtc aattgaaacc agaagtttat
    29821 gctgtttcat taaggttttc tttaagcttc atgaaaaaat gagcagtaag acaagtccta
    29881 tgttttttgt tatacttggg aagagaaagc taaagcaggt gaaggaatga aagtaaaagt
    29941 aaaaaaaaga gcaagcaatc gattgaaaaa aggatatcca ctgatccaaa aagaagacct
    30001 tgctcatccc caaaaagaaa ccacgcaatg ggtggaatta gttgattcgc aaaaccaatt
    30061 tatcgcaaaa ggatatctag gaaagcagaa taagggaatc ggttgggtat tgagccagca
    30121 gaacatccca tttgatcaat ctttttttga agaatttttt gtgaagtcaa aagaaaaaag
    30181 aactttcttt ttttcagatg attctactac cgcttttcgt atattcaatg gagaaggaga
    30241 cggaatcggc ggtctgattg ttgattatta tgcaggttat gctgtttttt cttggtataa
    30301 cgaaacgtta tatgcacatc gtgaattatt gattcaagct tttcgtgaaa tctacccgga
    30361 aatcgtagga ggctatgaga aaattcgatt tgaatcagta aatctgcctg agagccagtt
    30421 catttacgga aaagaggcac cggaaccctt gatcgtaaaa gaaaacggag taaaatttgc
    30481 cgtttattta aatgaaggat taatgactgg gatcttttta gaccaaaaag aagtacgggg
    30541 atcattagca gaaggtttcg cttctgggaa aacagttttg aacatgttta gttataccgg
    30601 cgccttttct gttgctgccg caatgggagg ggcaatcgaa acaacaagtg tcgatctagc
    30661 aaaacgcagt ttaaaaaaaa cacaggaaca gtttgaagtg aatggtttgg atgtcgatca
    30721 gcaaaaaatc attgttatgg acgtattcga gtatttcaaa tacgccaaaa gaaaacaatt
    30781 tttatatgac atgattgtat tggatccacc aagttttgcc agaaataaga aaaaagtctt
    30841 tcgcgtagca aaagattatg gggaactggt caaggatagt ctggatattt tagcagaaga
    30901 cgggatactt attgcgtcta caaatgctgc gaatgtctcg atagaacaat tccaaacaat
    30961 gattgaagag gaattccatg ccaaaaatgt ttcttttgaa caacttgctt tatatcgtct
    31021 gccaaaagat ttccagacga cggatacctt tttagaaggc aattatctga aagtgtttgt
    31081 ttatcaaatt aaaaataacc attagaagga gcgatgcaga atggtttggg taaagggatt
    31141 gaatatagga agcggtagac caaagatttg tgcgcctctt atcggggccg tccgagagga
    31201 agtattacgt gaagcagttc ttgcacgtga agcaggggcc gatttagtcg aatggcgagt
    31261 cgatcattat cgtgaagttt tttcttttga tgaagtagca gaaacattgg ttcttatcca
    31321 tgagatgctt gatggactgc cgttattatt tacgtttcgt accttagctg aaggcggaga
    31381 acaagacata tcgatccgtc agtatcgcga cctttatgaa catgtgatac acacaggatt
    31441 agtagatatg gtcgatatcg agttgttcaa agtagaaagt ctaggaaaaa gactgataga
    31501 ggaaatcaag tctttaaaca ttcctttaat tatcagtagt catgatttta aagaaacacc
    31561 agcagaccct gtcttgcttt accgattgaa tgttatggaa cattttggag ccgatatcgg
    31621 aaaactggca gtaactccca ataatgaacg agatgttttg cggttgatgg agttgactag
    31681 acgggcaaat gcttttgtct ccatgcctat tattacgatg tccatgggaa atcttggtaa
    31741 aatttctcgt ctggctggcg gaacgacggg ttccgttatg acatttggtt ccttaagcag
    31801 cgattctact tctgctccag gacaattacc ggtcaaagca ttgaagcaaa ttattcaatt
    31861 attaaatgaa ccgttgtaga acaagtttga ggtgatattt tttggcaaag aaaaaaatag
    31921 taaaaacgaa tgcaatccgt atggtcgaac aaaaaaagat tccctataca gagcacgaat
    31981 atgagtggga cgaaagccac ctaagtgcgt catcggtcgc tgaacagctt ccagaaagcc
    32041 aatctcgtat atttaaaaca ctggttgctg tgggcaatgt gacggggcct cttgttgctg
    32101 tgataccggg agaagccgaa ttgaatctaa aaaaattagc aaaagtttca gggaataaaa
    32161 aagtagaaat gctccattta aaagatttgg aagcaacgac cggatacata cgtggtggct
    32221 gctcgcctat aggtatgaaa aaactatttc ctacttattt agatcaaatt gcagaaagct
    32281 atgaacaaat cattgtctcg gctggcagaa gagggctaca gatggagtta gcacctcaag
    32341 atatctgtgc attaacgagc ggacaatttg cagatataaa acaataaatc atacgtataa
    32401 aagaaagaag gaggccggga tgttgtttgt ccagtctcct tctttctttt ttatacatta
    32461 tgctctttgc ccggcattca ttcctgctac atgccctgta gcgaaagccg ctgtgatatt
    32521 aaagccgcca gtataaccat tgacatccag taattctccc gcaaaataca gaccatcaat
    32581 gattttactc tccaatgttt taggataaat ttcctttaga gaaacccctc cgccagttac
    32641 aaatgatttc tcaataggaa aagttttgtt gattgatagc ttaaactctt tgcatagttg
    32701 aacaaaatct tgaatttgtt tttgagaggc ttgttgtcct gtcagatgat ccatttcaag
    32761 ccgctcaaga aagaaaacta acaatcgctc aggcaatata ccatgccaag cattgaccaa
    32821 gttctttttg gttgctttac ttttttctgt caatatatga ataagctctt gtttggtttg
    32881 tgtagggaaa caatccagag aaagtgtcac tggttctccc gttttctcta attctttatt
    32941 gacaaaactt gaacaacgca gtgcagccgg accagaaata ccgaaatggg tgaaaagtaa
    33001 atccatcgta tgttcagtca atacacgacc ttttttattt aaaacgcgta aagtgatgtc
    33061 ttgtaaagaa agcccttgaa gtgttttctc ctgaatatac ggttcatccg atatcaaggg
    33121 ggactctgtc gcataaagag gcgtcaccgt gtgacccact cgcttcacga tcttgtaccc
    33181 atctccagta gaaccagtag atggataagt ccgtccacca gttgttaaga taatggcagg
    33241 agcatggaac tcttcgaatt ccgtgcgtat accgtaaatt ttatgttctt tgtggatcaa
    33301 tttctctact cgagtagaga atagaagcgt cacatctagt tctttcagac gattgagcaa
    33361 tgcttcgata atcgtttttg atttatttgt tacaggaaac atccgtccat gatcctcttc
    33421 ttttaaatgt acgccttgtg attcaaagaa gttcatgata tcgaaattat tccattgcgc
    33481 aaacgtacta tataaaaact ttccatttcc aggaatatgt gcaatcaaat catcaacagg
    33541 acgattgttc gtaacattgc accgaccacc acctgtcatc agtagttttt ttcctgcttt
    33601 tttgtttttt tcgatcaaga gtacgcgtgc gccttgttca gctgcactga tcgctgccat
    33661 catcccgctt gttccagcgc cgactacgat cacatcatac agtttttcca ttccctcacc
    33721 tcaaggaaag tgtagcatat ctgaagaaag aaaacagaaa aaaaatgttt tgagccaaaa
    33781 gagaaaatga gctaaaatga gggaagataa gcaaaggagc gtggaacgat gacaaacgca
    33841 acagaatatg ttcaagcaat tcaagaaaag ctacatcaaa aggatcaagg gcaagaagaa
    33901 tttttacaag caatcgatga attcatgccc acggttatgt cttttttgga cactcatccc
    33961 gaatatatcg aaaagaatat cttagaactt ttaacggaac cagaaagagt tattcaattt
    34021 cgagtgcctt ggcaagatga tgaaggaaac tggcgagtaa atagagggta ccgtatccaa
    34081 tacaactcag ctatcggccc ttataaaggt ggattgcgct tccatcctag tgtcaatcta
    34141 agtatcttga aatttttggc ttttgaacag attttcaaga atagtttaac cggtctgcca
    34201 atcggtggag gaaaaggcgg aagtgacttc gatcctaaag gaaaatctga taatgagatc
    34261 atgcgttttt gtcaaagttt catgctggaa ttgtcaaaac atatcggccc atcgattgat
    34321 gttcctgcgg gagatatcgg tgtaggagct agagaaatcg gctatttgtt cggcgaatat
    34381 aaacgattga aaaaatatga tactggtgtg ttgacaggta aacctttgga tttttgggga
    34441 agtaagatac gtacggaagc tactggttat ggattagtgt attatgtcaa acatctattg
    34501 aacgaagaaa aagattcatt taatcaaaaa acagtttttg tttccggaag cgggaatgta
    34561 gccatttatg caatcgaaaa agtgcaagag ctaggcggga aagttgttac atgttcagat
    34621 tctagcgggt acatctatga tccagaagga atagatgtga aactattgaa agaagtcaaa
    34681 gaaatcaata gacaacgttt gacggaatac gcaaaccaac gtccttctgc tatttatcat
    34741 gctggagaat ctgtttggac attgaaagaa aaagcggata tcgctttgcc atgtgctact
    34801 caaaatgaaa tagacgaaag cttggccgaa atcttagtag aaaatgggat caaaatcgta
    34861 gcagaaggcg ctaatatgcc atgtacagta aaagctgttt ctgttcttca tgaagcaggc
    34921 gtgtggtatt gtccaggaaa agctgctaat gctggaggag tagcagtatc tgctttagaa
    34981 atgagtcaga acgtccagcg attgacttgg gaaagagagc aagtggatga gcagctggat
    35041 caaatcatgg aaacgattta ccatacgtgt cgccaaacag cagccgagta cggcgacgaa
    35101 aaaaatctgc ttcttggagc aaatgttgct ggatttgaaa aagttgccaa agcaatggca
    35161 gcagaaggtt tagtttgaaa aatgaaactt tcttggaaat attgtcagca ttttgtttat
    35221 ttgcttgaaa aataataaaa aaaaacgtaa agtattacct gtagagtaaa cctataagga
    35281 ggagaacaaa tggcacacat tcattttgac tattcaaaag tagcaccgtt tgtcaacgaa
    35341 caagagttag ggtacatgca aagccaagta acagcagccc acaacgaatt acgggaaggt
    35401 actggcgcag gaagcgactt ccgcggctgg atcgatttac caacgaacta tgacaaagaa
    35461 gaatttgcac gtatcaaaga agcagctaaa aaaatccaat ctgattctga agtattagtt
    35521 gttatcggta tcggtggttc ttatttaggc gctcgtgcag cgatcgactt cttgaatcac
    35581 acattcttca atttattaga tagcaacaca agaaaagcac cacaaatctt ctttgccgga
    35641 aattcaatca gctcaacata tattgctgac ttgatcgaag taatcggtga tcgcgacttt
    35701 tcagtaaacg ttatttcaaa atctggaaca actactgaac cagcgattgc gttccgtgta
    35761 ttcaaagagc ttcttgttaa gaaatatgga caagaagaag caaacaaacg tatctacgct
    35821 acaacagata aagcaaaagg tgcagttaaa gtagaagcag atgcagaagg ttgggaaact
    35881 ttcgtgattc ctgatgacat cggcggacgt ttctctgtat taactgctgt tggtttgtta
    35941 ccaatcgctg ttagtggtgc agatatcgat gcattgatgc aaggtgccgc tgatgcaagc
    36001 aaagcatact caagcgacaa actagaagaa aacgaagctt accaatacgc agcaatgcgc
    36061 aacattcttt accgtaaagg caaagtaact gagttgttga tcaactatga accaggaatg
    36121 caatacttct cagaatggtg gaaacaattg tatggtgaat ctgaaggaaa agaccaaaaa
    36181 ggcatctatc cttcaagcgc aaacttctca actgatttgc actctttagg acaattcatc
    36241 caagaaggac gtcgcaatat ttttgaaaca gttgtgaagg ttgaaaaacc tcgtaaatca
    36301 atcacaattc ctgaacaagc agaagattta gacggtctag gctacttgca aggaaaagaa
    36361 gtggacttcg taaatacaaa agcatttgaa ggaacattgc ttgctcatac agacggtgac
    36421 gtgcctaact tgctagtcaa aattccagaa atggatgctt atacattggg ttatacaatg
    36481 tacttctttg aaattgctgt aggtatttca ggttacttga atggaatcaa cccattcgac
    36541 caaccaggtg tagaagctta caagaaaaac atgttcgcac ttctaggcaa accaggcttt
    36601 gaagatcttg ctaaagaatt gaacgaacgc ctgtaagata agggtttttc gctagattta
    36661 aataatcagt gatagaaaca tgttcgcttt aaaatgaaaa ctggtaccca tttaggtact
    36721 caaacatact gcatcgttta ttcagaataa ggggctgtga caagacgttt gtcacagtcc
    36781 cttgtttgta tagaaatcga ttgattgcct tgctctttag tttgttttag gactttaaga
    36841 acagtgaggc tgacttcgaa ggttaattct gtttttttgt tttctagaat cagtaagcaa
    36901 aaaagcactc acttcgagtg cttttttgcg atatattctt agcttaagat aggcctaatt
    36961 tttcttttac agatgagggt accttatcgc tggtaattac ttttttctct atgatccgct
    37021 tttcgcttgc tttcaactgc aagatcgttc cttcggggta agcagaacca ttggcagagt
    37081 caccagaagt aatgacatcg aaatctaact gtcttttttc accgttttca gcataagcat
    37141 ctacgttgta tttaaaacct cttccaatta cttccccatt atctgctttg atatcagttt
    37201 ctgcaggcat tggagctttg actaccgcat aataatcttt acctacataa gtagactgat
    37261 agtagtccca acctttccat cctataaaag caatcaatat gataacgatc aaccctaaaa
    37321 tttttttcat gaaagatctc cttttttatt tttattatac ccgtggtgct agttaatttt
    37381 aatatcctaa agaatatctc aaagttcctt caaaacgtcg ttgtgctcga cttaatagaa
    37441 cactgtaagc cagtaatatg ctttcaaatc tactctctag ccctttaaca gaacgtgagt
    37501 tgaaattttg acaacctaat ttttctaagg aagaaaaaac agtctcaact g
//
LOCUS       DACDXE010000022        35710 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.022, whole genome shotgun sequence.
ACCESSION   DACDXE010000022 DACDXE010000000
VERSION     DACDXE010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.022"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(5..1282)
                     /locus_tag="IWM23_001118"
     CDS             complement(5..1282)
                     /locus_tag="IWM23_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883775.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWM23_001119"
     CDS             complement(1361..2005)
                     /gene="pcp"
                     /locus_tag="IWM23_001119"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAP9883776.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(2204..3148)
                     /locus_tag="IWM23_001120"
     CDS             complement(2204..3148)
                     /locus_tag="IWM23_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAP9883777.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(3150..3836)
                     /locus_tag="IWM23_001121"
     CDS             complement(3150..3836)
                     /locus_tag="IWM23_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAP9883778.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            4310..4630
                     /locus_tag="IWM23_001122"
     CDS             4310..4630
                     /locus_tag="IWM23_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAP9883779.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            4784..5689
                     /locus_tag="IWM23_001123"
     CDS             4784..5689
                     /locus_tag="IWM23_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883780.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            5686..6459
                     /locus_tag="IWM23_001124"
     CDS             5686..6459
                     /locus_tag="IWM23_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9883781.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(6640..8064)
                     /locus_tag="IWM23_001125"
     CDS             complement(6640..8064)
                     /locus_tag="IWM23_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883782.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            8275..8721
                     /gene="nrdI"
                     /locus_tag="IWM23_001126"
     CDS             8275..8721
                     /gene="nrdI"
                     /locus_tag="IWM23_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAP9883783.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(8786..10768)
                     /locus_tag="IWM23_001127"
     CDS             complement(8786..10768)
                     /locus_tag="IWM23_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9883784.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWM23_001128"
     CDS             complement(10782..11678)
                     /gene="murQ"
                     /locus_tag="IWM23_001128"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9883785.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(11681..12742)
                     /locus_tag="IWM23_001129"
     CDS             complement(11681..12742)
                     /locus_tag="IWM23_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9883786.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(12953..13480)
                     /locus_tag="IWM23_001130"
     CDS             complement(12953..13480)
                     /locus_tag="IWM23_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9883787.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(13547..15049)
                     /locus_tag="IWM23_001131"
     CDS             complement(13547..15049)
                     /locus_tag="IWM23_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9883788.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(15307..16428)
                     /locus_tag="IWM23_001132"
     CDS             complement(15307..16428)
                     /locus_tag="IWM23_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9883789.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            16554..17495
                     /locus_tag="IWM23_001133"
     CDS             16554..17495
                     /locus_tag="IWM23_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9883790.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(17645..18541)
                     /locus_tag="IWM23_001134"
     CDS             complement(17645..18541)
                     /locus_tag="IWM23_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9883791.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            18810..20150
                     /locus_tag="IWM23_001135"
     CDS             18810..20150
                     /locus_tag="IWM23_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9883792.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            20368..20811
                     /locus_tag="IWM23_001136"
     CDS             20368..20811
                     /locus_tag="IWM23_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAP9883793.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWM23_001137"
     CDS             complement(21315..22733)
                     /gene="nhaC"
                     /locus_tag="IWM23_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9883794.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(23136..24359)
                     /locus_tag="IWM23_001138"
     CDS             complement(23136..24359)
                     /locus_tag="IWM23_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAP9883795.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(24390..25229)
                     /locus_tag="IWM23_001139"
     CDS             complement(24390..25229)
                     /locus_tag="IWM23_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAP9883796.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(25246..25926)
                     /locus_tag="IWM23_001140"
     CDS             complement(25246..25926)
                     /locus_tag="IWM23_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9883797.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(25928..27001)
                     /locus_tag="IWM23_001141"
     CDS             complement(25928..27001)
                     /locus_tag="IWM23_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883798.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(27648..28319)
                     /locus_tag="IWM23_001142"
     CDS             complement(27648..28319)
                     /locus_tag="IWM23_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9883799.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IWM23_001143"
     CDS             complement(28406..31084)
                     /gene="mgtA"
                     /locus_tag="IWM23_001143"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAP9883800.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            31743..32180
                     /gene="ndk"
                     /locus_tag="IWM23_001144"
     CDS             31743..32180
                     /gene="ndk"
                     /locus_tag="IWM23_001144"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAP9883801.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(32202..33068)
                     /locus_tag="IWM23_001145"
     CDS             complement(32202..33068)
                     /locus_tag="IWM23_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9883802.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(33228..35321)
                     /locus_tag="IWM23_001146"
     CDS             complement(33228..35321)
                     /locus_tag="IWM23_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9883803.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        10343 a         7494 c         6296 g        11577 t
ORIGIN      
        1 ttttttatag taattccttc agatactttc ttgcctcatc attgaaacgc agtgattctt
       61 ctaacttctg ataatattcc aacgcttgtt tctgagcttc agtaaaacga taatgttttc
      121 ctttccattt gtcgattgtc tcttgcacat ttccagattg gatatctttc actactagtt
      181 gccatttttc ttttgagact gtacggtctt tcttatcagc agattgtata gcttctcgtt
      241 ctttacgcaa tacttttaaa aaaccatttc tgactatccg aggaatgctg tcgaagaacc
      301 cttctgtaaa accatagcga caatattgct tccaaataaa tagatagatt tttggttgga
      361 tgaatcgttg gtttttcgca aaatattcca tctgagcagt gacttctggt gttcgttgac
      421 gttttgtctt tctcaacacg gaaataaatg tttcaaaata agcattagga tgtactggca
      481 cttcttcata tacgatggat tgccaatctt tacgtaaaaa tgcgcgatag ttgatcaaga
      541 attcttctac atcctttact aattcttctg caaagtcgtc aaatagttct ttttttatct
      601 gtcgcttttt tgactgccca ttgatgtgtt cacttatttt cttgatttca ttttccaatg
      661 cttgataatc cgctatgtaa atcggcattt ctacttggcg aaattctgcg agccgctgag
      721 ctaattttat atccggtaaa tatggctgtt tcatccgacg attttggtac aagacaaaac
      781 agtatttttg ccatagtcct tcaggaaaat cgatattctt ctgcttaagc cataataatt
      841 cgtctaaggc cagatcaact tttgtcattc cctgatggat cgaagctgcc actgtcggag
      901 agacatgcag gtgatcggca aagtggttga tgcctagttt taggatcttt ccggtttttg
      961 gcgatttaac gatgtattgc acttttaatt ctctaccaca ctcacaatac aatttttgct
     1021 ggcttcgttt tgttcgaaaa aacggattga tcttgaagtc agaaaacacc caatctgttg
     1081 cagctaaata atgttcttcc ataaaacgag agctgattag atatcggcgg tgcttatcga
     1141 ctagttcccg ctgttttttc gttaattttg catagatctc ccaacgttga ctgcttgtgt
     1201 attgtttaga aattttgatg ggatagcttt tgctgatttc ttttggaaac cattcttcaa
     1261 agacggattg ccattcttcc acggctgttt ctttcctttc taattacaaa aagctggcta
     1321 ctaacataac gttagcaacc agccagtgtt tcttgctaaa ttagtgagtt gttccggcat
     1381 ttccggaaat atcttcttta ttttctactg ctgcttcaac tgcataagtc aaagaagaag
     1441 caatgtcttc taaagacata gaaggcgtac ccactggacg atcgatcact tgatcgggtg
     1501 agaatggcac gtggatgaac ccacctcgaa ttgccgggaa ttgtgtttcg atcaaatgaa
     1561 gcaaacgata catgatatca ttgcagacaa atgttcctgc tgtataagag atgtacgctg
     1621 gtagaccatg atcttgtaca ttttttgtca ttgctttgat tggcaatgag gtaaagtaag
     1681 cggctggacc gttttcttct aaagccgtat cgaatggctg atttccttca ttatcaggga
     1741 ttcttgcttc tgctagattg attgcgacac gttcgaacga gactgctgac cgcccacctg
     1801 cttgtccgac acagatcacg acatctggtt ggtgctgttg gatggcttct ttgactactt
     1861 gactgctttt ttcgaatacg gtcggtactt ccactttgat gatttcagca ccgctgattt
     1921 catctggcat tttttttact gcttcatagg ctggattcac tttgtcccca ccaaatggat
     1981 caaaacctgt tactagtact ttcatctgtt gttcctccgt ccgattgatt taacatatgg
     2041 ttatctttta tcataccatt ttccttgaac agcgtaaata aaggaaacaa tagtttaaat
     2101 aacataacag atcaaactta atatattctt ataaaaaaat agtgttccac tacaatgtta
     2161 gaagaggttg cgacaattat tctgtcacaa cccctctctt ctattattta aatagaatca
     2221 tataagcaat ttggaaaact agcataaata acgcaataaa caactggttt ttgatcactc
     2281 cgtatttgtc tttcatctcc agcatcgcta caggcacgat attgaagtta gcagccatcg
     2341 gtgtcagcag ggtcccacaa aaaccgcaag tcaatgcaac cattcctatt aacgctggat
     2401 tcgctccatg actaaatacg aatggtgcac cgatcccaac cgtcatcacg gtgatggccg
     2461 caaatgcatt ccccatgatt accgtaaata tcaccatgcc aagagcgtaa atgatgatgc
     2521 cgatgttcac atttccttct ggaacgatcg tacctactgc attggaaatc actgtcccaa
     2581 ctccagcgct tgtaaatacc gcacctagag aagctaaaag cattggcaac atgcttaacg
     2641 gccctactgt gcccagcata tctgctgcat catctaaaaa tgtacttggt cgattatctc
     2701 gagaaaagaa catcaagatt agcatggctg caaaaacacc gacgcccacg ccgactaaag
     2761 cacctaaatt ggtaaataga gcaaacaaga tcgcaaagac accgatactc aaagctggaa
     2821 taaaaatttt cattcctagt ttgtctgaca ttttttccat atattctttt gatggaagcc
     2881 ggctttctcc tttgctgact ttcttagcaa ttgctggtat ggtcatcgca aagatcaagc
     2941 caccgttcac gattgccgga agccatcttc ctcctgcaat aactacccct aagaccgtcc
     3001 agaaaaaggc tgacccataa cgatgaggat tggttttatc tgttaaattt cttactccag
     3061 tgtaaatcaa tacgagtccc atcacgatat aaaggatctc cagtaattta tcgcctaata
     3121 aaatatcagg atttgtaaaa aagtccatct tattgcccct ttcctggata atatttcttc
     3181 gctaatttct tttctttaag atagaagtag acacttgcca caactaaagc cgtgatagcc
     3241 actggaactt ccactaaagc aagctgtccc aatgttacgt catatcctag atctttcagt
     3301 gttgattgga ctagcaacgc ccctgctcct ccgatgaata gtacttggcc aaagaaccag
     3361 cagacatttt ccatagctga agccatacct tttagttctt cttcatgctt aggatggact
     3421 tctaaccctt ttgattcgat ggtccccaat gccatcggta aaatgattgg acgaacaaat
     3481 ccagcaactc caccaaaact cacgttaaaa gccgcaaaga tcattcgaaa gatcccataa
     3541 atatctacaa tcataccagc agatactttc ttgaatcgct tgatcaagga agccgctgat
     3601 tctttcagac catttctttc aagcgttcct gttgccaaca taatgatgat aaaaatcgcc
     3661 atcccgcgat tgtttacaaa acttgtacct aaaagttcta acattccttg aatgccaaga
     3721 ccgccagtca aagctgtaac aactaaagca accattacga tcaatatcga gtctaattta
     3781 aatgcaaacc cgagaataac aatcaagacg cctaaaaggc taagataatt ttccatatat
     3841 tttcctccat aaaatgacaa tattttttta attataaagc aaaacacata aaaaatatag
     3901 ttgttttctc acgaaataga cactattttt aaatattttt cgttattttt ttatcacaaa
     3961 aattatctta cttattgtaa ggtaaagctt tttttaattt attgcccgat aatatataga
     4021 aataaggtct agttactgtt ttttttaaat gattaataaa taacaacttc tttctatgaa
     4081 attgcaaagc attcatcttc ttttccgcta acgatatatc cgtcagtgct tggtacactt
     4141 tactaaaaag tatgagattg ccgatatctg gataggtgtg ctatactaca gaaaaagata
     4201 tacaatctgc caccgggtag gataaagagg atgatcattc cattaggtct ttgaagggat
     4261 gattttcctc tttttgtgta tttttttata gttaaggagg aatgtaagta tggcatggat
     4321 ggaattaatc atagcaggaa ttttggaagt attctggtca acgatgatga aatggagtga
     4381 tggtttttct aaaatcaatt attcttttta cacagtgatt ggcatgattg ctagttttta
     4441 ctttttgtca aaggcaatca aaagtttgcc gatgagtctc tcttatccta tctggacagg
     4501 gattggcgca gttggttcgg tcataattgg tgtggtcttg tttcatgata agctgaatct
     4561 ttccacttgg ttctttgtgg ggatgctact catcagtatc attggcatca agattactag
     4621 cggccactag acacttggaa taagtactga tcagaaaaaa taagcctttt tatttaatat
     4681 tcaatttttt tatgaaaaaa taagaaataa gtccaatgac ctagcgactt ctccactcat
     4741 caggtgtatg cttgtactat ctatagaaaa ggagtgaata tttttgaaaa gtctttcggt
     4801 ccaagaatta tccaagaact ttggtcacaa acaagccctc agcgatgtca gttttagctt
     4861 atcccctggc aaaatcgttg gattagtagg tcctaatggc gctggaaaaa caacgatcat
     4921 gaaagctatt ttaggactga tccattactc agatggaaca atctgtattg gtgaaaatga
     4981 gatttctccc tctagtcata aagggttgga acaagtagga gcattgatcg aatacccggg
     5041 tatctatcca ttcttaacag gctatgatca tctgaagtta ttctctgaaa ccaacgatgt
     5101 atctgctatc gacactatcg tgaatcagct caagatggaa aagtacattc acaaaaaagc
     5161 gaaatcctac tcgttgggaa tgaaacaaaa attgggaatt gctcttgcct tactgaacca
     5221 tccgtctatc gttattttag atgagccgat gaacggtttg gatccacaag caacaagaga
     5281 tgtgcgaaaa atcattaccg acttggctac tcagggcacc tctttcttga tctccagtca
     5341 cttactcagc gagcttgaaa aaatggtcga tgagctgatc ctgatcaatc aaggaaagat
     5401 catcaaacaa tgtacaatgg ctgaattgaa ccaaacagcg caagatttca tcgttatctc
     5461 tacaacaaat gatctagctg ctcgaacgat cttgacagaa gctggatata ctttggaaga
     5521 tcctgataaa atcaagcttt taaaagaaaa cgaagaatta ttaggaaccg tgatccagct
     5581 attgtctgca caaaatatcc atgtgacaga cgtccagcat ctgacgaatg atttggaatc
     5641 ttctctattg gaaattctgg attcagtaaa gggggaagaa gaaaaatgac attgcttgta
     5701 aaacaagaat tatttaaatt gatcaagaaa aagtcaactg ctgttttatc tgttctatta
     5761 gttgttttgc tcataggaac agcacttcta gccaaaaaat acacgacgat cattgatcca
     5821 gttgaaatga ctgctcagct ttttagtgcc acatcttgga ttgtctttat catgattgca
     5881 gctgcaagca cgatcatttc gatggaagca caatatggta cattgaaaaa tctcctttat
     5941 cgaaagtatt ctcgaggaga aatattagta agcaaatgga tcacattagt tatttattct
     6001 gtttatttat accttttagc tattattgtc actgtgttga tgaaactgat tcttttccct
     6061 tcgatttcct tcacggagaa ggtttctact ggtcaaacac tgatccaaag cttgttcact
     6121 tatactttgg gtagttatat cggtttatgg ctgattttaa gtttagtgtt gatgattgct
     6181 tgctttatca atagttccgg tgcatccatc tcagcaggaa tcgtctttta ttttgcttcg
     6241 tcaatcatat ctggtatcct tattgcactc atccaaaaat gggagtggat caaatggaat
     6301 ccgatcagta tgttgaatct gcaaaaccaa attggaaacg aagagatcat gaagcaattg
     6361 actcatcttt ctactaatca aatgctattc ggtaatttag cttacatcgt tttgttctta
     6421 gcattaggat atctcgtatt taagcgaaaa aatgtatgat gactcatcaa aaaagaggct
     6481 gtgaaagaga cgttcagcct tattgccgat tggctgtctc ttaaacaccg tttattctga
     6541 aaaaggagct gtgacaacat ttgtgtcaca gctcctttcc tagtatgttt ttgaataaac
     6601 gacaggctca caacctctct ttatacttct tttatacttc tatacgtgaa gcatcccagt
     6661 ggcaaataaa accattgcaa tcactccgac catcgtcaca cctataagta aaatcttatc
     6721 taagtggata tgttgtacag gctcgttttg ctccttacgt gctttgaggt aaaaataaat
     6781 gcctggcaag aaaagaacaa gaacaagcat tacttcatta aaggctacga ctagcatagc
     6841 ccatacttga aaggcacttg ctaaaagccc tactaaaaat tggacccatt ctttccgctg
     6901 ataagaaagt ttcatttggt acaatcctaa gaaaagccac gtgaagaaaa tagctgctgt
     6961 accaagtgtg taagcaaagt tataagcact gtcagtgaat aataatgaaa gtaaaaagac
     7021 attcgtacat aacgtcgtca atacgatagc ataagttggt gccccatttt tgttgagttt
     7081 tccccatgct ttaggcaaaa gtccgtcttc agccatgttt cccattgttt caatcggtaa
     7141 gatcgtccat gagagccaaa caccaactaa agaaatgatc aaaccgccgt tgatcacgac
     7201 tgctccccat ttaccaacca tcatttcaaa cacatagccc atagcaggct gcgccttgat
     7261 cgtttgcagc tgattcatcg tcattgtccc atatggaagc atggaaacca agacgtaaat
     7321 aataatcaag ccgaatatcc ctagaatcgt tgctttccca acatctgatt gtttttttgc
     7381 tcgtgaacct acgacagagg ctccttcaat accaacaaat acccaaagca tcaccatgat
     7441 gcaactcaat acttgactgg aaaacgggat cggctgtcca ttcgcattca atataatgtc
     7501 gctagaaaag ttatcaatga attgttggat cgaaaacgtg ctgatagcta ctacaataaa
     7561 taaaaataat ggcactaatt tacagaatgt gacgatagca ttgacgaaac tagcattttc
     7621 gactccccga ttcactagcc atgcatatcc ccataaaaag actgtactca tcacgatcga
     7681 tgccagattt tgcccacctt caaatactgg gaagaaatat cctaatgcgc tcatcaacaa
     7741 tgttgcaaaa gctacgttgc ctaaccagca tgtgagccag taaccccaac cgctgataaa
     7801 accagcgaaa cggccaaaac cttcttctgc gtaactaaag atcccgcctt ttaaatctgg
     7861 acgtttttta ttgatattat tgatcgacaa ggagagcatc aacattgctg ttcctacgag
     7921 tacccaactg aaaatagctg ggccgccagc cgatccggag atgtccgtca taataccaaa
     7981 tacgccacct ccaatcgctg aaccaattac taaggcagca agtggaatta aaccaattcc
     8041 ttttttctgt tgctctgtat ccatgtaagc atcctccaaa ataaaaccgc ttttatcaaa
     8101 aagtataaca aacctttttt atttttactt aattaataaa aactttaata gaaatataat
     8161 tctaccggct tttttctcat tatacaagca aagcgcctag tccctttcca aattacacca
     8221 ccaaaaaact taaaaaagat gctatactaa aaggaaagaa ttggaggatg atccatgaac
     8281 attttatata tatcgatttc aggaaatacg cgtgcctttg ccaaacatct agcagaatat
     8341 gctgaaaaaa tgcatgcaga agatcctgtc aatcctgaag ttactttaaa agaaatccat
     8401 gaaaatagtg attttacgaa agaaacagaa ccatttttta ctttcgttcc tacttattta
     8461 gacggcggaa atggtttaga taacggtgat acagaaatct tgaccgaaac gatgcgtgaa
     8521 tatctggaac atgaagacaa tcataaactt tgtcttggtg tagtcggtag cggaaataaa
     8581 aacttcaata atcagtattg tctaacagcc aaacaatatg cacaaacatt tggctttcct
     8641 ttcttggcag attatgaatt acggggaaca ccatctgatg tgacacgaat ttatcagatt
     8701 ttagcagaaa acaataaata ataaaaaaga agaagctgaa aaatagattt cagtttcttc
     8761 tttcgtttct attgtttcta tcatattaag ctttgacatc catcacaggt gcttttgctt
     8821 ttgctttacc tgttttttcc aagacaaagt tttttacacg atccatattg gttaccaaga
     8881 cgatcatgga tgtttctttg cctgctgctt tgatcgcttc taagtcagct tctgctacaa
     8941 gcgtatcagg tgtcacttgc tgcccttctg ttactttcac atcaaatggc gtaccgccta
     9001 agtcaactgt gttgattccc atatgaagca agatttctaa accatttggc gttgtgatcc
     9061 caatagcgtg tttcgttggg aagacagagc tgactacccc agtaactgga gaatagatat
     9121 gtccgttttc tggttctaca aagtagcctt cacccatcat cttttgtgca aaaactggat
     9181 cacttgcttt atctaaagaa acaactgttc cgtcagcaac tgatgagagg atagtcacac
     9241 tttcagctga agtttgaggt actacttttt caggcacttc atttactgtg gctgtttcag
     9301 tcgctaattc ttctttttct aaacggtctt taggaatacc gaagaagaat gtagcaacga
     9361 agccaccagc ataagctgcc aataatccaa ggatgtagcc taaccattta ccgtcagaga
     9421 tcaatgggat caaagcggct cctgaaggtc caatagcgat agccccgaca tttccgatca
     9481 ttccgatcac tgccccgccg atacctccac cgatacaagc agtaataaat gggcgtccta
     9541 gaggtaatgt tactccgtaa atcaatggtt cgccgattcc taggatcccg actggtaaag
     9601 ctcctttgat catttctgtt aattctttat cttttttgca gcgaatccat aatgccaatg
     9661 ctgcacccac ttgtcctgca ccagccatcg caagaattgg aagcaatagc gtacgaccag
     9721 tttcatcaat catttgcaca tggattggag tcaaaatttg atgcagtccg aacataacca
     9781 tcggcaagaa taatgtgcct agaacgaagc cggcaaatgc tccaccaacg ccaagtaccc
     9841 agttgatccc accgaccatg ccatcagaaa tgaaacctgc taaaggcatg atgaagaaaa
     9901 tttcaatcag accgatggca atcaatgaaa gagttggtgt aacgatgata tctaccgcat
     9961 caggtactac tttatgcagt tttttctcaa cgattgacaa tagccaaaca gcgaagatga
    10021 cacctaggat cccaccttgt ccagctgcaa gagcttcacc agtaaataag tttttcagtg
    10081 gtgcttccgg attcatccct gtcagcatca caacggcccc gataactcca ccaagagttg
    10141 gtgttgcacc aaaaacttgt gcagcattga tacctgtata gataactagg tagctaaaca
    10201 tcccgttttt caagatgttc atcacatcga tatattgttg ccatgttgct gcatcaagtg
    10261 ttccggctgt gactaagttt gctaaaactg cggcaatccc tgccacgatc ccagtaccga
    10321 caaatgcagg aatcattggc acaaagatat tagaaatgtc ttttaatacc gatttgattt
    10381 tagatggttt ttgtttcgat ttttgtgccg ctttcatttc ttgtgcccgt tcgtttacac
    10441 ggtcttttcc agatgctgat gaatttgctg tagctgtact tacagaagaa agtttttctc
    10501 ctaatttcac gcctgccaaa tcaaccattt gctgtgctac tttgttcact ttcccaggtc
    10561 cgatgatgac ttgaagctgt tcatcttcta ctacaccaag cactcctgga atcgctttta
    10621 attcagagac atttacaaga tcatcattga tgatcttcat acgcacacga gtcatgcagt
    10681 ggacaatgct ttctacattg cccatgccgc ctgccccttt ataaatttgt tttgccagtt
    10741 cttccaaagt cagttcgttt tttgccatgg tcttttcccc ctcataaggt cccttttaca
    10801 aatccgtttg cttcagctaa ttttgcttga gcttcctctt tatcactatt tgtcaaaatc
    10861 atgacgatag ccagcttaac attttgatct gcttcttcaa agtacttttc agctgtttcg
    10921 taatcagctt ctgtcgcttg catgatgatt cgttttgagc gttcgattag tttttcattt
    10981 gttggtttga catcgaccat caagttattg aatactttac ctataccaat catactgata
    11041 gtagaaatca tattcaagat aagtttttgt gctgttcctg acttcaatcg agtagagcct
    11101 gtcaaaaatt ctggacctgc atcgacttcg attgggaaat ccgcgtgttt actgatttcg
    11161 gcttgtttat tgcaagaaat cgttcctgtg acagcaccga tcgattttgc gtaatctaat
    11221 ccaccgatca catatggtgt acgaccgcta gctgcgatac caataactat atcatttttt
    11281 gtcaaattca gcttgattag atcttcttgc cctagctcat aagaatcttc ggccccttcg
    11341 acagctacag tcattgcttc ttctccacca gcaattagac ctatgaccat ttccggttct
    11401 acaccgaatg tcggtacgca ttcagcagca tctaacacac ctagtcttcc acttgttccg
    11461 gctcccatat aaatcagacg tccacctttt ttaaaggact caatcgtgtt agcaatcact
    11521 ggttcgatac ttgtcagttc tttttcaacc gcagcggcca ctttctgatc ttctttgttc
    11581 attagttgaa cggcttgaga aatactcatc tcatctaatc caaaagtttc ttggtttctg
    11641 cgttccgttg ttaggttctc taaattgatt ttgctcattt ttatcttcct tcttttctta
    11701 taaattgata gttcatacca gctttgattt ggttgatcaa tggtagctct tctttgataa
    11761 ctttagctac tacattcact ttttcatccg ctggcaaatc atatttggtt aattggatct
    11821 cacccatata gcgcaaatac ttttcgttat ctaaagtaat agaccctttc atacgcgtag
    11881 ctgtatttct tgctgggata ggcggaatct ctcgaaaacg tgcatctacg cttcgtatga
    11941 catctcttgc atcgtcttgc cgattcgtat gcttccctaa tatatattcg taaaattctt
    12001 catctacagg ttccgtatgg agcaagactg tctgttcttt ttggaaagaa gcaaattgct
    12061 cctgaacgtt ttctgacaga cctgcatctc cgatgtaaac aatatccgtg ttacttgcta
    12121 atagatctag agcgcctgca agaggatgga tccctcgatg ttcttccaaa gttggcaatc
    12181 cttgatatag tgggcctctc aactctgtat ctcctggtac aaaaccttgg acaaggagac
    12241 cttgaatttt gagccattga ttttttttct gataccattc tttgtccagc cctgtttcgg
    12301 gacgtggata ataattatgc caagcttcaa tctgtgagaa atcagcttct gcttctttca
    12361 attcatcgat atctttaggc gtgatcgtgc tagcattcaa actgattttc aattgatgtg
    12421 accagtcggc aatctgctga ttggaaatat gataatccat tcttaaacct gttacaccaa
    12481 ttgctttcaa aggcgttaga tcttctaatg aaaaacccgc tcgttccaat gcctctcctg
    12541 aaatatcgac catcaattct agtttattct ttttcgcttc gcttcctagt tcgatcaatc
    12601 gcttttgata tgccgaaaca tcatcttctg gtatatgcat ggaagtgaaa ataccgacaa
    12661 agccattgga tgccatttgg cggatatact gtttcttctc ttctgacaaa gaatcgttca
    12721 taaatattga aaagccaaac atattatccc tctttttctg tatccgtctt catttctatg
    12781 actagagtat atatctaaag aaacaaaatt tcaatacttt tattaaaata attaaatttc
    12841 atttctaaag gttgagttta tattatcaaa agaattgtca taacctaaaa aaggcactgc
    12901 taagcaaaag cagtgccttg aaatatttta tttaaataac acaaaaacat aattactcat
    12961 aagaaaccaa ttctgtatta tctaccatga atgtaaagaa cccctcatta ggcatcggat
    13021 gaaaataagc attgatcact cttatcaagg cattatggca aactaacaga tagacatttt
    13081 cttcgtcttc gatgcattct ttcaataaag gaacgatccg tgcatacaca tctaagactg
    13141 actccccatt tgggaatcgt acagcgaacg ctaaacgtgc tttttgaaag ttctcatctt
    13201 tgcttggcag tccatcataa tccccaaaat ccatttctac caatcgttca tcaacggtca
    13261 gcggcaagct gagacgatca gcaactgctt gtgctgtatc tcttgctcgt tgaagagggg
    13321 aatggatgat tttcgtgatt ggaacaggca aatctactac cttttctgct agtttctgtg
    13381 cctgcatctg tcctttttca gtcagcggca catctgcccg tccgcaaact aatccttgtg
    13441 cgttccaagt tgtttctccg tgtctcgtta cataaagcat gtgattctct ccttcgttgg
    13501 caaagttccc cttaaccata ccttgttgtt cttgagatta caagggttag attctctcaa
    13561 gagaaataat ctcagtagat actttatcca ggaaggtttg atcatgttcc actagtaaca
    13621 tggcaggttt cacgttcaag atcagttgtt ctaattgttc ttgattgaag acatccaaat
    13681 aattcaatgg ttcatcccat gtatatagtt cagctggctg tgataaagat ttagccaatt
    13741 ccactttttt ccgttggccc atactcatct gctcgatctt gttatgaaaa acatctcttt
    13801 ccatcccaag ctttcggagg ttgttcaaaa atgcttggta gtctacttgg tttttctctg
    13861 caaattccgc caacgttcct cgattgtctt cataattttg gcttgcataa ctaatgctca
    13921 gatgttttgg ctggtatttt tctcctataa ccttgccgtt gaatgccccc agaagataat
    13981 ggataatgga tgactttcct gcaccgttag gacctgaaat cgccacccgc tgatgaggct
    14041 cgattgtaaa atgaattggc tcgaataaca gattttcata ccctaattga agatcttcta
    14101 cgcttaaaag tcgcttatgg tgagacgctt ggctattcat cgtcaaagaa tcgatatatt
    14161 cgatatcttt taatagtttt tctttctcgc tgatctgggt ctccatccga ttcacgattg
    14221 ctttggatcg tttcatcgtc cgtgcagcat cagcacccac tgctccttta ttcactcgtc
    14281 tagattcagt atcgatgaat ccgacttgtt tctttgtttt atctccttct cgggaacgag
    14341 accattcggc tttttcagct gctgttttct ttagcctact gacttctttt ttcaattttt
    14401 cattttgagc catttcaaat tcatcacgaa gttttttctg ttcttcatag atagagaaat
    14461 tcccttgata aagttccagt tgactttttt cgattgctaa aacatggtcc actacttcat
    14521 cgataaatcc ccggtcatgg ctgaccacga tgaagccttg ttttttcttt ttcaaataag
    14581 ccgctacttg ttttctacca gagatatcca aatgattcgt tggttcatcg attaacggga
    14641 aatgagtgtc atccacaaat aaaagtgcca gtaggacttt tgtcttctct ccccccgata
    14701 gtgtgctgaa ttctctccat aagatttcag gatctgtttg catcaattgg agctctcttt
    14761 cgatttccca tatctcaaaa tccgtaatat catttaacac gtaataggtt aaacgttctt
    14821 tatcttttgt ctgttgcggg aaataggcaa attcttgctg atggattact tgcccttgat
    14881 aaggtagttt gttttgtaga atattcagta aagttgtctt tcctcgaccg tttcgtccga
    14941 taagtcctag tttccattgt gtgtcaaaat ttagatttgc ttgttcaaat aataatgtgc
    15001 cttggctgtc gtagccgaat gtcagatttt ttatttcgat tttagacatt ttccatcacc
    15061 tcgtaaaaaa agaccagtcc tattttcaga actggctaaa gaggatgcaa gggttggata
    15121 cacacaaaaa gacccataca tcgaaaaaac caagccaatc ccgaaaatct gcacactgct
    15181 tccttgtctc cacaaggtac agtctgaatg cagctttcat caatcgactt aatttttcaa
    15241 tgcacggatc cctcactttg tcctatattt gaaattacga tagcataaag aaaatgattt
    15301 tgtcaactac ttcgggagga tggtgaaaaa tctgctaagt gtctgtccta agatttttgt
    15361 tttttctaat gtttgcctgt gttccattgc ttcttctaaa cctattggac ccaaatgaat
    15421 acttaagaca attgggagaa aatcagaaag tccttctagc tcttggtctc tacttccgca
    15481 aatagcgatt attggttttc gatatttttt tgcaagcact gctacaccgt atggaacttt
    15541 tccgtggatc gtttgctgat cgatccgccc ttctcctgta atgatgacat ctgcttgttc
    15601 gatctttttt tccaatccag tgatttggct gactaaatcg aaaccagaaa ccatcttcgc
    15661 tcccaacaaa aaaagggctc caccaatacc gcctgctgct ccacttcctg gaatatcatc
    15721 taaatccacc tcccagaaat cttttatttg ctgagcaatc tttcctgctt gtttatctaa
    15781 gaaagctagc gtcgtatcta ttcctccttt ttgtcttcca aattgccaag cagctccttg
    15841 ttgacctgta tatggattgg taacatctgc agcgataatc aattttactt tagaaacaag
    15901 aacgtttctt cgatcaaaat gaagtttttt agtagataat aaaggatttc cttctgtgta
    15961 cccttctaaa gaagcaccca aggattggag taatcctaga ccaccatcgc tacacccgct
    16021 tcctcctaaa gtgacaataa tttgttctac ttcttgaagc agtgcatctt taataagttc
    16081 tcctaaggaa taactggaag caagtcggat agtttgatcc gttggtttat caagtagatc
    16141 taaccccaat gcttgtgcag attcgatgac tgcggtgttt tttccttgcc aagttgtcaa
    16201 taaatagctg atttttcgct gacggaataa gagatccatc ccatcaatgg tacgccatgt
    16261 tcccctgagc gcgtaataga ttgcttctgc agtattttct ccgccatctg caatcggcac
    16321 attgattttt cttcctttac gatcttccca gccatttaga actgcctgat ttagttcaaa
    16381 tgaagaagca cttcctttaa atgaatcaat tgccgccaaa atatccatct gtatccctca
    16441 ctttcttctt aagtaagaca ctttttttca tttttttcaa acaaaacaat gctattttct
    16501 tccatttttc tatatactaa aagggaactc attgaataaa ggagagaaaa aacatgaaaa
    16561 tcgcaatagc tggggccgga gcaatgggca gccgaatcgg attgatgcta catcaaagcg
    16621 gaaatgaggt cctcttgatc gatcgttggc ctgctcacat tgaagccatt cgtacaaatg
    16681 ggttgattgc tgactttaac ggtaaagaag tggtcgcaaa actgccaatc tattcaccag
    16741 aagaaatcat cgaaagcaac gaacacgtgg acttgatcgt agcattaaca aaagccaatc
    16801 aattagatga tatgttttgt tcgatccaat ccattattac tgacaatact tatgttttgt
    16861 gtttactaaa tggattaggt catgaagatg tactggaaaa atatgtgcca aagaaaaata
    16921 tcttatttgg gatcacgatg tggacagctg gtctagctgg tcccggaaaa gtcacattgt
    16981 taggtgatgg agaaatcgaa ctagagaact tggaaccaga aggcgaagca tttacaaaaa
    17041 aagttgtaga agtctttcaa gaagcaaatt taaatccgat ctatagccat aacgttcgtt
    17101 attccatttg gcgaaaagct tgtgtaaatg gaacattgaa tggtttatgc acgattttgg
    17161 attgtaatat tgctgaacta ggcgcgcaaa aagcggcaga gagcatggtt cgaacaatcg
    17221 tatctgaatt tgcttctatc gcagcaaaag aaggaatcat actagatcaa gaagaagtgt
    17281 accaacatat tgcttctact tatgatcctg acaacatcgg gttgcattac ccatctatgt
    17341 accaagacct gatcaaaaac catcgtttga ctgaaatcga ctacatcaat ggtgccattt
    17401 ggcgaaaagg acaaaaatat gatattgcta caccatattg tgcctttttg acccaattag
    17461 tccatgcaaa agaaggaatc cttggcgcag aataaaaggc tctctgatga gaatcagctg
    17521 aatcagcagc tggttctctt tttttaagcc caataaaaga tcaaaaagaa aacacaactc
    17581 tcatgatgcc ataagaattg tgcttgtttg tttcagtaca taatctcttc aaaatgtagg
    17641 ttttttattt gttttcaaca gaggagaggt catattcgta ttgctcataa atagtggaat
    17701 cgttagtgat aaatcgtttt gcccaatttt ctgcatcata aaattccaat agctctgata
    17761 ggattggctt aaatggtgta tgcagaaatt ccggtaattc atctaaatca cgttctcgaa
    17821 gcaaaaaaat attttcagga cgataaaaat gataatgagc aatcgcttga ttatccgtca
    17881 ctagtttctt ttgaaaatac atggattcaa atgtccgcaa agtcactcct tgctgaccct
    17941 cccgcaacag ttccagaatc ccgcgcgatt cccctgtttt tttcaggtac tcttcataag
    18001 taagatatcc aggttgttca ttccctcgat gtggaagaat gaaatagttg gtcgtaacat
    18061 ctctttttat aaattcctgc ttatagcttt ctgctcgttc ttttctccca ttattcgttg
    18121 caccaaaaaa tagatccgtc tttggttcgc tagaagaaaa attcatatgt ttcgaataaa
    18181 atgaactatt atgtttcagc ccaagttctt ttgcttccaa aaaatcaaaa tgataaatct
    18241 catcgattgc cggttcatct tgtgcctgct tcagcatcgc ctcgtgttct gctttgaagt
    18301 gattccaaaa aaataacaca atttttgcat tggtaaatgg gcgcacgcgt cgagccatct
    18361 ccatatcaaa tccataatct aaaaaaacga tcagctgata atttgccaac tgttgtctag
    18421 ctgatttaat gaattccgac tgtagtaaat cttttccgga ccaaacatct aaagagcgga
    18481 tggaaggcaa aaaataaagt gatttatttt ttgcaactag cagtacgtca tttttgttca
    18541 tcttttccgc ctcactttct ctgtcacttt ttcctatgat agcctgttta aatagtaaaa
    18601 agccagtaaa acgtttccta tatttgtatg aaaatttaac atttgtatga aaatatgatg
    18661 aaataaatca ttcttctatt aaaattcgcc taaaagtttt ctgaatagtt gtaattgtct
    18721 gaaaatttgt tatcattagt cattgttaaa tattgctatt tattcttaat ataaattttg
    18781 attgtgaatt taaaagaaag aggtcattta tggaagataa aaaattgaaa aaacaactta
    18841 gctccagaca tatcaccatg ttagctttag gtggagcaat cggtgcaggt ctatttaaag
    18901 gaagtggcga agcgattggg atcgccggcc cttctgtatt gattgctttt ttgattggtg
    18961 ggctgatttt atatatcgtg atgaaaggtc tgggaaaaat cgttttaagc ggtggcgaca
    19021 cgcatcacgg attatctggt ttgatacgcc cttatctagg tgctcattct gctgatttta
    19081 ctgattgggt ctactggtct atgtggatta tcaatatcat cgctgaagct gttgcagctg
    19141 cttccttttt gcagttgtgg ttcccgcaca ttccagcctg ggtatttgtt tttatgttgg
    19201 ctgttttaac tacgatcatc aatctttatt cggtacggtt attcgcagaa acagaatatt
    19261 ggctggcttt tgctaaaatc agtgtcatta tcttactgat catttttgct acttatcttg
    19321 tcggtcagca aatgttgggc acaggcgttt tccctaccct tcagcaattg actgaccatg
    19381 gtgggtttac tcctcatggt atgaagggaa tcatcagttc tttgcttgta gttatctatt
    19441 cttacggcgg ttctgaactg attgctatca cggtaagcga agcagatgat cctaaacgtg
    19501 cgattcctaa agcaatcaaa ggtgtcatgg gaagaatcat ttccttttac atcattcctt
    19561 tgtttttgct tttgattctt tttccttgga atactttagc aggcacgaat gtcagtcctt
    19621 ttgtgatggt atttgaaaaa atgaatatcc catttgcagc ggatatcgtc aattttgtga
    19681 ttgtccttgc tttgttctca tctatcaatt cgggtgtgta tgcatcttca agaattttgt
    19741 tcttccgttt gaaggatcga aaaggttcct ctagaaaact agctgtttta aacaagcatc
    19801 aagtaccgca acgtgctgtt ttattttgta ccagtgttct ttatcttggt gtagctcttt
    19861 cttattttgt cggtgacaaa ttatttggct atctggcagg ttccctttcg tatacagtat
    19921 tattgatctg gatcattatc agtgcggcat cctttgtact ggcactaaga aaaggaacat
    19981 tatgggataa aggaatcagc ttttttgcat tagctgtctt aggattgatt ttccttggta
    20041 tcctgttcac gaactccatt ggggtaactg tattgactgg cctgctttac ctgttcattt
    20101 atttcagtta tcaaaaaaag aatgatgcgt tcgtattgac gaatgaatga aatcgataga
    20161 aaattttatt ttatttatgt ggaatagatg gagctattga agaaaggagt tgtgacaaaa
    20221 gtcttgttgc aactcctttt tccgaataaa cagtctcatg acctcttggt aaacggtgtt
    20281 caaaaaccct ctgtgcaaaa taattgattg ttctccatgc tcatgacatg atagaagaaa
    20341 ggaatgaaaa aaaggagttt gttttaaatg aaaaagtatt ggaagttttt gttgatgatc
    20401 ggtgtttcaa cggtagttat gtttggtatg atgtacttga atgtctatgc gctcgatcat
    20461 atcttcttca gtcgtacacg agtattcatg gcgttgatga tgggtgcagt catggcagtc
    20521 atcatgctgt tgttcatgtg gaaaatgtat gaaaacaaaa agataaatgt tatgatctta
    20581 gccgctagtc ttcttgtttt tgccggttcc ttgtttatgg tacgaagcca aactactgtc
    20641 aacgacacag cttggatgaa ggccatgatt cctcatcatt ctatcgctat cctggcaagt
    20701 aaacgagctg atttaaaaga tcctgaagta aaaaaattag cggaagaaat cattcaggca
    20761 caagaagaag agatcgacaa aatgaaaaaa atgatcaaag aaatggaatg accattatcc
    20821 gtccaaataa aaaaagagga tgttccagaa gttatcagct tcaagaaata agaagaagct
    20881 acttctggaa cgccgtgaat aaggggttgt gaaaaagctg ccctgcatca agtaataaga
    20941 acagccaaac aaaaatagct tctcctattt ttcgtttggt taggcttatt accgcagatg
    21001 caagcttttg aacaccgttt attaagaggt tgtaaaatca gcgttctgac ctgagtatta
    21061 gagagacaaa tggaaaaata gttcctcata ttttgccatt ttttgagcta ataccgaggg
    21121 tcagctgatt gaacaccgtt tatcgagagg ttgtgaaaaa gtcgcttagt ttcgcgtatt
    21181 aagaaaaatt ttgtgaaatg gctttccaca tttttgtata aatttggctt aataccgaga
    21241 aactggcttt tgaataccgt ttattcgtaa aagggattgt gacaaaagtc ttgtcacaat
    21301 ccctttttat gtttttattg ttctaattgc gctgtcttgc ttactgcttc ttgcggtttc
    21361 acaaattttt ttgagattcc ccaacctgtg aaggcactga ccatcgaaaa cacaggagaa
    21421 agcaaactga aaaatacgaa tggcaagtaa gtgagtgtag gaataccaaa tgtacttgct
    21481 acgaaacttc cggcaattcc ccaaggcact aagtagttga ttactgttcc accatcttct
    21541 agcgttctac ctaatacagt tggatcaaat cctgcttttt cataagtttc tttaaatgca
    21601 ttaccaggca gaataacaga taggaattgt tcaccaataa aaatatttac tccgatactt
    21661 gtcatcaatg ttgtaaaaat caaatttggt gtgttcgtca tctttcttga tactcgttcc
    21721 atcacggttg agatcaagcc aaattcgatc aacaatccac ctaatgacaa agttagaata
    21781 atcaatgaca cagtaggcat catactttcg attcctccgc ggctaagcag tgaatcgatt
    21841 tgttggtttc cagtatttga aacaaaccct gattcgatga ccccagccaa cttcgttaag
    21901 gaagtttgtg ggttttgaat aaaaatcatt cctactgcta cagcgatatt caacaaaatc
    21961 gttaaaattg ccggcacctt tttccatgca cacagtaaca ttaaaaataa aggaatcagt
    22021 gaccagattg aaatagtaaa gtttgcttct agaacatcag tcacttcacg aatagatgaa
    22081 agacttgcgg ctttacttgt gtgtcctaaa atcatgaata aaaagaaaga aacaacaaat
    22141 gccgggacag ttgaccacat catatgcttg atgtgtttga acaaatcggc atcaacaata
    22201 gctgctgcta gatttgttga ttcggataat ggagacatct tatctccaaa aacggcacct
    22261 gaaacaatag ctccaacaac cagcgctgga tttaagccta gtgttgtccc aataccgaaa
    22321 aatgcaatcc caatcgttga cataacagta aaggcactcc ctacagcgct acctacgatt
    22381 gcacacacaa caaatacaga agggacaaac cactggacac tgatcaattt gaacccaatg
    22441 accattagag ttggaatgat ccctgcttgt atccatacag caatcaatga accgactaaa
    22501 ataaaaataa agattgggat gattcctggt ttgatgccat tgatgattcc ttcgtgcagc
    22561 caatctgtcg ggtatctttt tatcactgca tacaacatga ccattccgat cgctgctaag
    22621 atcgtgatat taggtgagac ctttaatccg acaacgccaa tcgcaatgcc taaaacaata
    22681 accattaata caagtgttgc ttctaaaaaa cttggtgttc ctttttttcc catgataatc
    22741 tctccttttt tagttgttct tatgttctaa aaaagttaag atatcggatt actccgccgc
    22801 aattcatccg cgattcctcc tttataaaaa taaaaagacc tgttttaaac taaggacgaa
    22861 tgactccgcg gtaccacctt aattaaaaac agatctgttt ttcactttca aacttttatt
    22921 caaatgtatg gactttccat gtaattcgtc aattaggctt gatcggttca cagcgaccac
    22981 cgactttctg aacaaaacac ctaaccgcta ctgcgtgaaa agttgacttg tacgaagttt
    23041 accttccata agatcatttg tcaatgaaaa agaaaatatt ttttcatttt tctgaaaatt
    23101 atttttataa ctttcacaca caagtttatt ttttactaga aagtagcgcc ctcttgtttt
    23161 tttcttgcta ctatttcttg atgttcttgc ttgattcttt ctaatagctt tggctctttc
    23221 aacagatcaa gagctgtatt ggcgagtagt tctgctgcaa tcgcaataga agcaaggcct
    23281 ttgtcacttt tagcagcagc tttgaattct tctgtgtgtc cggcaatata tgtctcagag
    23341 attgctaccg taggctggat tgttggtatg acttggctga catttcctac atcagaagaa
    23401 ccgagacttg ttctttgttc cgtttctatt tcttcatctg gaacacctgc ttcttttgca
    23461 tgctggaaga ataagtcatc aaaagaagga gtaacgatga catcatcaac tgcattttgg
    23521 aacaatccaa actcaaaagt cgtgcccgta cttatcgcag cacctcgtac gatattttcc
    23581 actttttcat atactttatc taatgtttgc cgatttcctg cacgtaaata gaaacgtcca
    23641 gcagcgtaat ctggaatgac gttggcagct actcctccgt ctgtaatgat cccatggata
    23701 ttgacatctt ttggcaattg caagcgtaga gcgttgattc cattaaacac ttggatcaat
    23761 gcttccaaag catttactcc tttttcaggt gctgctgctg catgggaagc gacaccgtaa
    23821 aatttgatgt cgactggatc attcgctaaa gattctgtcg ttttcccata acgaaaagct
    23881 ggatggacgc ataacgctac atcgacatcc tcaaaaaaac cttctcgaac aaagctgcct
    23941 ttagctgatc cgttctcgcc cccttcttcc cctggggtgc catatacacg gatttctcca
    24001 ccagtttcat caatgaattg cttcactatg ctggcggcta atacggagta agtaccaaac
    24061 aaattatgcc cgcatgcatg accaatacct ggtaaagcat cgaattctgc caaaaaggca
    24121 attgtgggac cgggtttatt ggaaatataa cgtgcatcaa aggctgttcg gtgtccagct
    24181 acttcttttt ctacccaaaa cctttctttt tctaattgtt taatcaacgc tgctgaggaa
    24241 taaaactcat agttacttac ttcaggatgt tcgtggatat ctagagcaag tgcttgatat
    24301 tctggtaaac gatattggat tagatccttc aattggtttg ttgagattgt tgttgtcata
    24361 aaaaattcct cctatgctag tcatttttct tattttctcc cgaatgtttc ccatgcaggc
    24421 acgcttgatc cttttgatgt tttctcgatt actttgactg tgtcttctgt ttgataagcg
    24481 tctactattt tttgatacgt tttattgttt tcatcttctt ttcttgcaac gatgatattc
    24541 acataaggac gtgcatggtc atccactggt tctaagaaga tggcatcaga agcaggatta
    24601 aatccagcat ctacagctac accgctattg atcactgaaa tgtctacatc tgacaaagca
    24661 cgtgctgttt gggaagcatc taattcagaa atgtctagat tttttctatt ctcggtaata
    24721 tcagaaacag tgggcgcttg tccttttgcc ttatccactt tgatcaatcc agcagtttgc
    24781 aataacagaa gagctctgcc gccatttgta acatcattag ggattgctac ttttgctccg
    24841 tctttgatat cagctacatc cttcaaagtg ttagaataaa ttcccagcgg tgcattgaca
    24901 gtatttccga tcgatactaa atcagttccg tgttctttat tgtaattgtc taaaaagaat
    24961 tgatgttgga aagaattcaa atcaatcgat ccatctgcta aagccacatt tggttgattg
    25021 taatctgtaa atttcacgta ttccaaatta atgttctcct ttttcaaacg gtctttcacg
    25081 ttgtcccata catctgtatc ttctccaata atccctagtt ttacgatagt ttcttttttg
    25141 tcagttgtat ctgcttttcc ttggctacac cctgccaacc aaataattgc tacaaatgta
    25201 atgactacaa aacgaataat ttttttcatt ttttctcctc cactttcaat gtgtaatttt
    25261 tttaatcagt tgattactga taaattgact taaaaaaact aagattagaa tcaataatgt
    25321 agccacaatc gttacatctg tttggaatcg attataccct ctcgatatag caagattccc
    25381 taatccgcct gcacctactg ctccagccat ggtagtcagt ccgatcacgt tgatgatagt
    25441 caacgccgag acccgaatga taccaggcaa accttctaac aaatacacac gtaaaatgat
    25501 accgattgga ctggttccca tcgcttctgc cgcttcgatc accccaggat caacttctag
    25561 cagtgcgttc tcgatttgac gagcaaaaaa cggaacaact ccaaaaatca atggcactaa
    25621 agctgctttc gcaccaatcg tcgttccaac taagaaacga gtagtcaatg ctaaaagaga
    25681 aagcaagata atgaaaggga tggaacgaat gatattgatc agcttatcaa gtatttcaaa
    25741 tagcagacgg ttagctagta atccattagg gcgtgtcact actaacgtca cacctaagat
    25801 gattccaatc atcccgccaa tgaatgctgt ccaaaaagac atatatagtg tttgcttcgt
    25861 cgcttcgaca aattcatccg gaatcaaata aacattgggc aaatatgttt taaagaaatc
    25921 agtcatttta tcttcctcct tcgatcactt tgatacgact atctaaatcg tttttttgta
    25981 aaatatttat ttttacacca gatttgatca aataatccaa tgcttttttc cgctgtttgg
    26041 tttctccagc taaagtaacg atcaaactcc ctaacggcgt ttcttgtaat aactctacat
    26101 tgccatataa aatattcgct gttacttgat atttgctgta cagatgagcg attagaggtt
    26161 cgttcgtctg atttccaata taacttaatt ccaccagcca tttattggcg atctgatcgg
    26221 caaaagcaga atgggcaatg atcgtttcta atgcttggtc aagatgagta gctgttcgaa
    26281 taaaatcttt ggtcaattct tcttcaggat tgctgaagat ctggatgctg ctaccttttt
    26341 caacgattct tccatcttcc ataaccgcta ccttgttgca gatttctttg acaacttgca
    26401 tctcatgagt gatcaggacg attgttaaac ctaactggcg attcaatttt tttaacaatg
    26461 caaggatctg aagggtcgtt ttcggatcaa gtgcactggt tgcttcatca cataataata
    26521 ccttaggttc attcgccaaa gctcgtgcga ttgctactcg ttgtttttgt ccccccgaca
    26581 gttgtttagg ataagcagat gctttttctt ccaaaccgac aagttccagt agttcattca
    26641 ctttttgacg acgttcctgt ttcgattttc cagaatactt taaagaaaaa tcaacgttgt
    26701 caaaaatagt tcggctttcc attagattga aatgctgaaa gatcataccg atggcctttc
    26761 tttcttttcg caattctttg ggagagagct gactcagatt cgtctgattg acaatgactt
    26821 ctcctgctgt tggtttctgc agcaaattca tcacacgaac taacgtactt ttccctgcac
    26881 ctgagtaacc gacgattcca taaacatcgc ctttttcaat cgttaaatct acagaatcta
    26941 ctgcctgtat ctctttttct ttttgtttga aacatacaga aacatctcgc aattcaatca
    27001 ttaaaattcc tccttatcta aaaaaagcgt cctcaagaaa atgaaaatgt cattttcttg
    27061 aggacgcttt tttgcgtgtt accacctcat gttcaagctg tgttcacaca caactcctct
    27121 tcaagtacga tcatactcta gcaagatatc ggtcgcagcc gtcatcgcct tacatctatg
    27181 ctcgggatga attgctcgat ggccattttc caatagtcgt tccttgcttc ttctcagcta
    27241 ccgaagctct ctgtcaaagg ttcttctatc gtacttacca tttcaatgca aataatattc
    27301 aaaaaaaaag tccttttact aacataaact atgtcagtaa aaggacgacg tatcgcggta
    27361 ccaccttttt tcggaagttt ccttccctca agcccaacat ctcaaaaaga gagattggcg
    27421 ttcgttttaa cggtcgaaca gttccgaatt tgcctactta tcgacaaatt attctccaag
    27481 gccattttca gcttttctta cttgactctt ctcaccagcc agagcctctc tgcaaagttt
    27541 ccaaacttac tttccttttc aacgaaatcg atattttgtt gttgggatta atctacatga
    27601 ggaaaactga aaagtcaagt acgaaattca aattattttt tctatattta tttttctgtt
    27661 tttttattta acatttcaat aacagttcga agatcttttc cttccgcaag aagtttcaat
    27721 ttttcctttt cttcgacggt ataatttcgg acaaaactgg aatcaggtat caaaacaacg
    27781 cctaatcctg ctcgtgaagc agctgttgct cctactaaag aatcttccac tgcaaaggct
    27841 gaactagctt ctaatcctgt tcgttccaaa gctgtcagat aaggaatcgg tgaaggcttg
    27901 ttctccgaca cttcgtctcc gaatgtaaac gtgtccaagt aatcagctaa tttaaagtga
    27961 tccaagatat ctgttgctct tttcgtaaca gtggtcgtag caatgcctag tttcatatga
    28021 cgttctttta agtctgccaa tatttctttt gcatatggtt ttaagtggat ggctccgctt
    28081 gtcagctgtt gataaaaata ttcttcacgc ttcgctcgga cttcctgtac tttttcaggg
    28141 ctacctgctg ctttcactaa aatatcaaaa ctttgtttcc agttttttcc gccccaagcg
    28201 tcaacgattt cttttgggat atggatatca aattgctgaa atccccaaag ccagccatca
    28261 cgatacgttt tttctgtgtc aactaacaaa ccatctaaat caaatattgc acctttcatc
    28321 tatacattaa tcctttccaa aacaatacac atagtttagc acgactggtc caaaaagaac
    28381 agaagcaaag aaccagcctt ctcgtttata agagttcttg gaatcgacgg atatagattt
    28441 tcttcacagc tgtcacaagc gccagataag cgatgactgt agcaaataac cataaccagt
    28501 aattgccagg aagtggcatc aagcctaatt cagcaccaaa acttgtaaaa ggaaggacag
    28561 aaccgacaat aattcctaat gtagtgataa caaacatggc aaatgttgcg ttgctttgga
    28621 taaatggtac tttcggtgta cgcagtgcat ggagcactaa ggtttgtgtc catagtgatt
    28681 cgacaaacca tcctgcatgg aataatgcga tgaaaatcac tcgctgtgaa ctatccaatg
    28741 tatggaaact tccgcccact acagcaggac aaataacaaa atacatcaac aagtaagtag
    28801 taatatcaaa tatcgaactt gttggaccaa agtaaaccat gaattcccca atactagatg
    28861 cattccattt ttttggctcg tccaaatatt ctttatccat ccgatcccat ggcaaggaaa
    28921 tgcaggaaac atcatagatc agattcaaaa acaaaagttg taaaggcagc attggtaaaa
    28981 atggcaggaa cgtacttgca atcagcacgg aaaacatatt gccaaaatta gaacttgctg
    29041 tagccttgat atatttcatg atattcccaa aagtttctcg tccagaaaga aggcctcttt
    29101 ccaaaatcgt caaatctttt tctaaaagga tcacgtctgc agattctttc gcaatatcca
    29161 cagctgtatc tacagaaata ccgatatcag ccactttcat tgcaggagcg tcattgatac
    29221 catctcctaa aaatccgaca gtatgaccat ttttacgtaa agctgtcgtc aaacgtgctt
    29281 tttgctgagg attcaatttt acgaatactt catggtcttc agctacttgt gctaattcat
    29341 gatcactcat ttccgagatt ttctcaccgg taaccaattc attgatctct agacctactt
    29401 gacggcacac tgaacgtgta actaattcgt tgtccccagt cagaactttt acagctacac
    29461 catgttcttt cagagctttc aacgcttgtt tcgttgtttc ttttggtgga tcaaggaaag
    29521 caagataacc aatcaaaacc atgtctgact catctttgac tgaaaattct ccaacgacgc
    29581 ttggattggt cttttgtgcg acaccaatca ctcgtaagcc atcttcatta agtgactgta
    29641 cttttgcaag tacggcttct tttcgttctt ttgttaatgg tgtaacagta ccgccaagat
    29701 caataaattt agaaatgttc agcatttctt caatcgcgcc ttttgtgatc atttgtgttt
    29761 taccagctgg atcagcaacc acaacactca tacgacgacg ttcaaaatca aaaggaattt
    29821 catccacttt ttgataattg atcttatctg tatctaaagt ttctgttgct tcatcaatga
    29881 tagccacatc taacaaattt ttcagccctg tttgatagta gctatttaga tacgcatggc
    29941 gcaaaacacg atcatcttca tttccttcac aatccatatg atattcaaga atgattttat
    30001 cttgtgtcaa tgtccccgtt ttatccgtac ataacacatc gattgcacca aagttctgga
    30061 tggcattcaa atttttgatc actgttcctt ttttggccat cacacttgct cctttgacca
    30121 aattcgtcgt aacgatcatt ggaagcattt caggtgttag accaactgct acagatagtc
    30181 caaaaagaaa agcttctagc caatcccctt ttgtaagccc attgatgacg ataactgctg
    30241 gagccatcaa tgacataaat ttgatcagta gcatggatgt tttatggatg cctaattcaa
    30301 agcttgattg aattggtttt tcagatagcg tttctgctac atgaccaaat agtgtttcat
    30361 ttccaacaga aacaatgatt ccttccgcac ttccactgat gacattactt cccataaaga
    30421 caagattttc gtaacttgtt tcgctttgct gatcgtcttc acatactttc gcatgctttt
    30481 ctactggata gctttcccct gtcaatgctg cttgggaaat gaataaatcc tttgcggaca
    30541 ataaacgcat atccgctggt atcatatccc ctgctgaaag ccgaacaaga tctccacaga
    30601 cgatttcttc cataggaatc tctgtgtact ttccattacg ttttacagct gccgttactt
    30661 tcacaagtga tttcagtttt tcagccgctt gattggaacg tacggactga atcaacgtca
    30721 ttgtcccgct gacgataacc atagtgaaga taatggctac accgaaaaga tctttatctc
    30781 caggagctgc taatacatat tccgtgatga atgaaataat tcctaatgcg atcaacacta
    30841 atgtaaatgg cgtgatgtat gctttcaata tttctacaat gaatggtgtt tttttgccat
    30901 atgaaatcgc attttcaccg taattttctc ttgaacgttc gatttgttct tctgtcaatc
    30961 catttgctga tgcatggaat agttcaaata attcttgttt cgtttttcca gcaaaagctg
    31021 catagatttt ttgtgtatct ttcttttttt ccatctcaat ctctcctttg tgtccaaaag
    31081 acagctgttc tcgctgcttt tggtctttag ctattcatcg gatcgataga gaaaaaactg
    31141 aaaggggctg agaacagata gataatagcc ctggcctttc ctctagatcc ttgtttttcg
    31201 aatgactgtc atccatttgt tctcatctcc tttactaagt aattttaatt catgacttcc
    31261 aatcattttt cgaatagtta tttttccaat tagaccacct cctttttatt tttcaccgat
    31321 ggggatttgc ggcaacaaaa aaacctctcg ccaaatactc agcgaaaggt ttgaaaaagc
    31381 atattaaaaa aagcttctca gatctcatcg ttgagttttg acactgtaca acgtaaaaag
    31441 cagatacctg cttttagcta ccttatgaaa agccttatct cgacaattcc tgttctaccc
    31501 attggcgtct ctcgacgttt ttgggcagta gcctatgttt gtataggagc ctcacctaac
    31561 aaatcttatt cggttttcct tcgttaatat aacaactaaa aaagaggatg tcaaatcaat
    31621 tttccatgtt tttcatactt tttttaaaat acacacgtta tattcctgtc tcacgcggtt
    31681 ttatttgtct cattttccac ctcccgctat aatagaaata tagtaggaaa cgaggtgctg
    31741 ttatggaaag aacacttgtt attatcaagc cagacggcgt gagacgccat ttagtcggaa
    31801 gcatcatcca acgttttgaa gcaaagggac tggccattgc tgaaatgaag tttgaaacta
    31861 tgactcctga attagcaaaa gaacattatc agcacttgac tgagcgcagt ttttttgatg
    31921 aactgatcga ttacatgact tccggtcctg tcgtttatct tgtgctggtc ggcgaggaag
    31981 tgatcgatat cgtccgtaag atggttggag caacaaaagc tgccgatgct gttccaggta
    32041 cgatccgagg agactacgcg ctgccgggga ctgaaaacat cattcacgct tctgattccc
    32101 gtgatgctgc ggtaaaagag atcgcccgct ttttcccgcc attcgatata aacaaaccgt
    32161 ctgaaaaagc ggcgaagtga tacaaattct aaactgctgg tctagttgct gggttcagca
    32221 atggccagca gttttttctc taaggacctc cgttgattct ttcctaacat atattcgagc
    32281 actgtgaaga attcttggta gtctttcgtc ttttcttccc ctaaagcagc agaaatcaat
    32341 ctttgataat aatctgccat caatgtttca tagatcactg tacttctttc tttatgtgct
    32401 aagctccgtt tcatatcttc attcacacta cgtgcctttt tcaaccgacc attttctata
    32461 taaaacgaag tcaggttcat taaaaaagta aagagatact gctcatgatt gttcaactgt
    32521 gctaatcgtc gcattctttt tttgattttc aaataattgt tctcaatgaa ccgatcactg
    32581 aatatccaag gaaaatcagc tagaatagat agttcaaatt gaccatagtc tggtatctgc
    32641 atcaagtatt cttcaatgat tcggcattca taacgtaaaa attcaaattc tgtctcagat
    32701 acttctgttt tttcgaccat gtgaagattc aaaaccaaat aatacagatc ttctgttttc
    32761 ttaaaatgcc tacggttatt gctgatctct ttataaggat caaagctgtc ttcttgtacc
    32821 cgatctagca ttcgtctttt tttctttaac tttattggtt catccagctg gtttcgcatc
    32881 cattcatatt ctgcaagtgt catgttcatt cgctctaaaa atgatctcaa tgttacaaat
    32941 gacatttttt tcactgaatt tgtctcaatc ccgactagag aagtcttaaa acagattcct
    33001 tccgctaatt gggactgtgt caacttcttg ttgactcgaa gtttcttgat caattcacca
    33061 tcatacataa tagttctcct ttttaattta aatttttcca cattcaataa atagatacgt
    33121 taaaaaggaa aaatacacga aaaaaataaa aaaatgtttt tttcataaaa aataccaacc
    33181 ttcaagcatt agaatgttgt ctaactactg aaggttggtt tgattgttta ttgattattt
    33241 ttttgctgtt caacgtctgt tcttgttact ggatataagt tggttgttgt tttattacca
    33301 ttttttgaaa agacacttgt cgaatgttcg cctttttgct tttcgattgc ttcattacgt
    33361 tccgcttcat ttttatagtc atattttttc ggatcaatcg tctcaaatcc gtttggcaca
    33421 taaaaccgca agagattttc ctgattcagc ttatctgaaa gacttaacgc agtcttgact
    33481 ttttcctgat ctttggagat ttcctcatcg attcctgctg ctttcgtatc gatgatttct
    33541 cctgtctgat tattgtaagc ttttcctcct aggacggtgt atttgggtgt cacaaagttc
    33601 ccatttcgaa aagcaactac ttgatcatgc tgagaagata ataagtctgt accaaaatga
    33661 agataatctt tatcatcgat tcccaataga tgtagtaatg tcggcaaaac atcgatttct
    33721 ccaccgtatt catgattgac tgtcccttta gtataaccag gcatatggat catcaatggc
    33781 actcgttgca tctgtgcgtt gtcaaattca tcccatgtat ctggatcttt accaagagcg
    33841 cttgctaaat ctttattatc tgtattcgag atcccaaaat gatccccgta aatcacaaag
    33901 attgaatttt ggtaaatacc agattttttc aaatacgtaa aaaattgttc caatgattga
    33961 tccagataat gtgccgttcg aacataatta tctactgtgc tattgccagt attcaaagat
    34021 ggaaagtcga aattcttgtc atccgtataa taaggcgtgt ggttggttac ggaaagaaat
    34081 ttcgtgtaaa acggctgttg tagatgctca agatattggg cagattctct gaataaatct
    34141 ttatctaata tcccataacc taacgtttcg tcggattcat caaaataaga tcgatcaaag
    34201 aaattgtcat aacctaagtt tttatataca tgatctctat tccaaaaact agctacattc
    34261 ccatggaaaa cagcgcttgt atagccttgc ttttgtccaa ggatcgctgg tgcggcttga
    34321 aagacattgt ctgacccaag ttctgtgaat aatgagcctt gaggtaaacc aaatgtgcca
    34381 gtttccaaca tattttcagc atcacttgtc tttccctgtc ctacttcatg aaagaaattg
    34441 tcaaagctga tcgttgcttg attctggaaa atactattta aaaacggtgt gacttcttgt
    34501 ccgtctacct tcatattaat caaaaactgc tggaaacttt ctaaatggag aacgatcaca
    34561 tttttccctt tagcgatccc aaatgtttcg ggatttggct ctgcataatg tttctttgta
    34621 taatctaaaa cttcatcaat cccattactg cttgcatgcg cgcgaacact gctagtcatt
    34681 cctgttttga ttccgtcata aatagtatac gcatcgattc ctagatattt gacaatatat
    34741 gaacggtcaa atgttcgctg cagcaattgt ggacggttgg cttcgctcag cgctagattt
    34801 cctgaaaaaa taagccctga aagacaggta acagcgatag ccatcggttt gccgacaggg
    34861 ttgcttttga taggtatttt cttgaccttc aaccaaacca gtagcccaat gaacacagcg
    34921 atatccagcc agtagatgac atcatggggc ttcatcaaag aaaaggagct tcctgacaat
    34981 ccttgcgaca ctttcgaaaa gcctaataca gatttgactg taatgaaatc tgtgaattcc
    35041 cgataaaaaa taatattcaa gtacaacaaa agcgtattta aaatatcaat gatcaaaaga
    35101 atagctaacg ctggctttgg ctttttaata tacaaagaaa ggccaaaaaa caataaggta
    35161 gtcgcaattg ggttgatcca caagataaag tactggatcg taccttccac acctaatgaa
    35221 aaatctgcat agtaggcaat gatcgtcttc agccagaaaa agaaaacagc tgtcaaaacg
    35281 attgctaaac gattttggca caatttttta agttgtgtca cgtatatcac ttctctctat
    35341 tcacattttt tcttattttg ctcctgaaaa atgaaaattt cctaaaaaag ttgttatctt
    35401 aagatttttt tattctttct aaagattatt ccgtccactt ttcacagcat aaaaccgctg
    35461 cgatgtaagc gattaaacta atgaagagat aaaagtcttg tggtgaccgc ttcactccac
    35521 ggtattcaaa agccagtttc tcggtattcg tacaaaaaaa gaggctgtga caaaattctt
    35581 gtcgcagctc cttatttctt gatgcaggac agctttttca caacttcttc cttaaacggt
    35641 gttctcctgc caactcctcg gccacaagtc acaatattta taaaacatga gaagctgttt
    35701 tttgaaattg
//
LOCUS       DACDXE010000023        34635 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.023, whole genome shotgun sequence.
ACCESSION   DACDXE010000023 DACDXE010000000
VERSION     DACDXE010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.023"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(208..2100)
                     /locus_tag="IWM23_001147"
     CDS             complement(208..2100)
                     /locus_tag="IWM23_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883804.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2097..3824)
                     /locus_tag="IWM23_001148"
     CDS             complement(2097..3824)
                     /locus_tag="IWM23_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9883805.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3840..4490)
                     /locus_tag="IWM23_001149"
     CDS             complement(3840..4490)
                     /locus_tag="IWM23_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9883806.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4694..4990
                     /locus_tag="IWM23_001150"
     CDS             4694..4990
                     /locus_tag="IWM23_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9883807.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5162..5782
                     /locus_tag="IWM23_001151"
     CDS             5162..5782
                     /locus_tag="IWM23_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAP9883808.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5874..7040)
                     /locus_tag="IWM23_001152"
     CDS             complement(5874..7040)
                     /locus_tag="IWM23_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9883809.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7052..7405)
                     /locus_tag="IWM23_001153"
     CDS             complement(7052..7405)
                     /locus_tag="IWM23_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9883810.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7533..8555
                     /locus_tag="IWM23_001154"
     CDS             7533..8555
                     /locus_tag="IWM23_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAP9883811.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(8994..9428)
                     /locus_tag="IWM23_001155"
     CDS             complement(8994..9428)
                     /locus_tag="IWM23_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883812.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9538..11397)
                     /locus_tag="IWM23_001156"
     CDS             complement(9538..11397)
                     /locus_tag="IWM23_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAP9883813.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11515..12675)
                     /locus_tag="IWM23_001157"
     CDS             complement(11515..12675)
                     /locus_tag="IWM23_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HAP9883814.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IWM23_001158"
     CDS             complement(12982..14406)
                     /gene="araA"
                     /locus_tag="IWM23_001158"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAP9883815.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14422..15120)
                     /locus_tag="IWM23_001159"
     CDS             complement(14422..15120)
                     /locus_tag="IWM23_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9883816.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15077..16738)
                     /locus_tag="IWM23_001160"
     CDS             complement(15077..16738)
                     /locus_tag="IWM23_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAP9883817.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            16956..18356
                     /locus_tag="IWM23_001161"
     CDS             16956..18356
                     /locus_tag="IWM23_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAP9883818.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18608..19687)
                     /locus_tag="IWM23_001162"
     CDS             complement(18608..19687)
                     /locus_tag="IWM23_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9883819.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19773..20732)
                     /locus_tag="IWM23_001163"
     CDS             complement(19773..20732)
                     /locus_tag="IWM23_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAP9883820.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20744..22267)
                     /locus_tag="IWM23_001164"
     CDS             complement(20744..22267)
                     /locus_tag="IWM23_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9883821.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22277..22840)
                     /locus_tag="IWM23_001165"
     CDS             complement(22277..22840)
                     /locus_tag="IWM23_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9883822.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22846..23709)
                     /locus_tag="IWM23_001166"
     CDS             complement(22846..23709)
                     /locus_tag="IWM23_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9883823.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23723..24619)
                     /locus_tag="IWM23_001167"
     CDS             complement(23723..24619)
                     /locus_tag="IWM23_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9883824.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24794..26065)
                     /locus_tag="IWM23_001168"
     CDS             complement(24794..26065)
                     /locus_tag="IWM23_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9883825.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26055..27572)
                     /locus_tag="IWM23_001169"
     CDS             complement(26055..27572)
                     /locus_tag="IWM23_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAP9883826.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27770..28660
                     /locus_tag="IWM23_001170"
     CDS             27770..28660
                     /locus_tag="IWM23_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAP9883827.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IWM23_001171"
     CDS             complement(28733..29557)
                     /gene="nadE"
                     /locus_tag="IWM23_001171"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAP9883828.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29569..31041)
                     /locus_tag="IWM23_001172"
     CDS             complement(29569..31041)
                     /locus_tag="IWM23_001172"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAP9883829.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31678..32040)
                     /locus_tag="IWM23_001173"
     CDS             complement(31678..32040)
                     /locus_tag="IWM23_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9883830.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32263..33015
                     /locus_tag="IWM23_001174"
     CDS             32263..33015
                     /locus_tag="IWM23_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9883831.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33155..34078)
                     /locus_tag="IWM23_001175"
     CDS             complement(33155..34078)
                     /locus_tag="IWM23_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9883832.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34093..34365)
                     /locus_tag="IWM23_001176"
     CDS             complement(34093..34365)
                     /locus_tag="IWM23_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883833.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10245 a         7204 c         5953 g        11233 t
ORIGIN      
        1 ttttgaacac cgtttaagga agaggttgta aaatcagcgg tttgacctga gtattagaga
       61 tacaaaacgg aaaaatagct tctcatattt tgccgttttt tgagctaata ccgaaggaca
      121 gctgattgaa caccgtttat tcggaaaaag ggactgtgac acgacttttg tcacagcccc
      181 taaaaaaaat aataaatttg tactattcta tgaagcttct tcttcagaaa actgactgtt
      241 ataaagatct gcatagaatc cgtctttttc catcaatgta tcgtggtttc ctgtttctac
      301 gatcgaacca tgattcatca caataatgtt gtctgcatcg cgtattgtag acaagcggtg
      361 agcaacaacg aaacttgtcc ggttttccaa taggcggttc atcgcttttt gaatcagtat
      421 ttctgttcgt gtatctacac tagatgttgc ttcatcaagg atcaggacat ctggatttgc
      481 taagaatgcc cgcgcaattg tgattagctg acgctgccct tgcgaaatat tgcttgcatc
      541 ctcgtttaat acagtgttat atccttctgg caatttacga acaaaatcgt ctacgtgagc
      601 agctttagca gcctcaatga tttgttcgtc tgttgcatcg tcgttcccat atttgatatt
      661 gtcatagata cttcctgtaa acagccaagt atcttgcagt accattgaaa actgtgcgcg
      721 caattcatca cgtgaaagat cacgggtatc tacaccatcg taacgaatac tacctccaga
      781 aacatcgtag aaccgttcaa gaaggttgat cagtgtcgtc ttccctgcac cagtcggacc
      841 tacaatagcg accatctcac caggtttgac atccaagtta aaatcagtca ttagcaagtt
      901 atctggtgtg tagccgaatt gaacattttc aaaacgaact ttgtatggtg tatctttttc
      961 ttctggaagg ccagattttt cattggacat ctcttcttca tccaacactt caaatacccg
     1021 ttcggctgaa gcaactgttg attggatagt gttcatcaaa cttgcaagtt gagtgattgg
     1081 ttgagagaac tgtgttgtat attgcaagaa cgcttgaaca tcccctaatg tcatgttccc
     1141 atttgcaact tttactccgc ctagtacagc gacgaacaca tagcctagat ttttaacgaa
     1201 attcatcaac ggcatgatga ttgccgaaat aaattgagct ttccaaccag cggcatacag
     1261 attctgattt tctttttcaa atacttcctg gtctttttca gtatgattga atgttttaac
     1321 aatggtatga ccaccatatg tttcttctac ttggttattc aataaaccta aacttttttg
     1381 ctgatctgcg aaaaacactt gtgatttcgg cgctacgatc atcacgatga tcaagcttaa
     1441 agggatcatc aataacgcga ctaacgtcaa ttgccagctg attgaaagca tcatcacgat
     1501 gaccccaatc aatgtgactg cactagttac aaattgagtc aaattttgct gcagcgtgct
     1561 tgcgatgtta tccatatcat tgattgctcg agacatgata tccccatttg tatgtgtatc
     1621 ataataggaa atcggtacac gattcatttt ggcttccaaa tcatgacgta attcataaac
     1681 tgttcgttgg gaaacacgtg tcataataaa ttgctgtaaa aagttgaata cagcagaaat
     1741 caaatacatc acgattactg tcgcaatgat ctgcgcgatt ttatcaaagt ctatcggaaa
     1801 ttttgttact tgttggcctt gttgcatctg ttgagcgcca gccattacac ctttgaatat
     1861 ttctgtggtt gctttcccca agacctttgg tgtttggatt tggaagatca cggctgctat
     1921 tgctagtaca aatacagcga tgatcgcaac catacgttta gacatatagc caaacaatcg
     1981 gcgtaccgtt ccccagaaat ttttaggctt ttcacctttt acaccgagat ttctgccagg
     2041 tccaccatgt cctggaccgc ctgctggtcc atgagaggga cgtgtgtttg ttttactcat
     2101 gctaagtcct cctctcttag ttgtgaatga acgatttctt gataagtttc gtttgttgct
     2161 aatagttctt catgtgttcc tttgccaacc agttttcctt catctagcac aaggatcata
     2221 tctgcatcca ttactgtact tactcgttga gcaactaaaa cggtgatact ttctttcata
     2281 ttcgttttca atgcttggcg aagattggca tccgttttga aatccaacgc tgagaaggaa
     2341 tcatcaaaca catagatatc tgctgttttg actaatgccc gtgcaattgc cagacgctgt
     2401 ctttgtccac cagagaagtt tcctcctcct tgttccacat ggctatctaa gccatcttcc
     2461 atctctgaaa caaaatcttt agcttgtgcg atttctaatg ctttccaaat catttcatct
     2521 gttgcatccg gcttaccata ctgcatattg tctcggatcg ttccagagaa cagaactgct
     2581 ttttgaggaa caaagcccat taattctcgc aaattatatt gagtcatgtc actaatgtcc
     2641 gttccattta tttggatcga accagattcg atgtcatata atcgagggat caaattgact
     2701 aaggtcgttt tacctgaacc agtcccccct atgattgcga cgatctcgcc cgacttggct
     2761 tggaaatcaa tatcttccaa tgcaagtttt tctgctccat gatatcgata attgacatga
     2821 ttgaaggcca atgttgcgtt ttttccagca aaagaaactg ttttaggatt ttcagggtca
     2881 tggataccga ttttttcatc caacacttca ttaatacggt cagctgaagc ttgtgcacga
     2941 ggcaccatga taaaaatagc ggaaagcatc ataaagctga tcaagatctg catcgcataa
     3001 gtcataaatg caatcagatt accgacttcc aaagtcatat ctgctatgta gtgtccacca
     3061 aaccaagtga ttgctacgtt tgtaccgctc atgatcaatg tcatcattgg catcatcaat
     3121 gcgacgatcg tattaacttt gattgccgta tttgtataat ctttattcgc taaatcaaaa
     3181 cgattttctt caaagcgtgt cttatcgaat gctcgaatca ctctgacccc tgtcaatcct
     3241 tctcgaaata ccaggtttaa acggtcagtt ttcttttgca tacttttgaa taatggaaca
     3301 gcaaagtaca tgattccgcc gatcaaaata atcattactg ggatgacaac taagaagatt
     3361 tttgtcaact gatgatcttt ttgataagca agtatgctcg cgccaatcaa cgtgattggt
     3421 gcattgatca tcatacgtaa aaacatttgt gtaaccattt ggatttgagt cacatcattg
     3481 gtcgttcttg tgatcaaaga ggccgttcca tatttatcaa acgcatcttt tgtcagactc
     3541 tctgattttt tatagacatc cgtacgcaat tgcttaccca gtttctgtga ttcacgagta
     3601 gcaaaaaatg tatttgcgat agcagctaag atactgatca aagaaaagcc gatcatcaca
     3661 aaacctgtat gccaaatata atcgacatct ccttgcgcta cccctttatc aataatattg
     3721 gaggtaagtg tcggcaaata taaatctgcc agtacttgga tcaccatgaa aactgcagct
     3781 gcaattgctg aagtcaagga gatccgtttt acgattttta gcatctagtt cccttctttc
     3841 tatttatata ctccgttttt cagccaagaa agtttcaaat tgaattcact gattgcttgc
     3901 tctactgagt aatcttcaga tgttgaaaat aagcgatacg aatgcgcaat tgttgtaaat
     3961 acagtattca tcactaattg gatcagtaat tccaattcat gatcattttt tactttcagc
     4021 ttcgtccgat cgatcaattc aagcaactct tttgcatctt gtttgtgttc tttatgtgga
     4081 tcgtgaggcg ttttttggcg actcgtatga ggagcgactt ttcttgaggc acggtaatcc
     4141 atgttcataa acaagttacg ataaaaagaa gcattctcgc cttccaaaat ctcataaatc
     4201 attttagaaa aataggattc gaatcctttg aatagatcac catcggattc tttcattagt
     4261 tgtaacatat ctttagagct gtcacgtctc attgtctcaa agtaataaaa ataaagatcc
     4321 tctttatctt caaaatactg atagaaactg cctctaggga tatccgctaa tttgacgata
     4381 ttagcaatcg aagcttcttt taagggtact ctagaaaact cgattctcgc agcctcgatt
     4441 agtctttttt gtttttcttt aggcaaatgg aaaaatgtct gtgtcggcat atggatcctc
     4501 ctttctttca agatgacaca ttgtcatatg acgccgtgtc attataacac ctcctacctg
     4561 ttttgcaaga catttgcata gtttttttca ttatcatccg atacattaaa aaagtgataa
     4621 gaggaatcct tccatttgaa ttaatgagag ttgcccatta aaatagccac atactaaaca
     4681 aaggatgaga taaatggccc aatcaattag tgtccaagac ttcaataaat tgcctctcga
     4741 tgctgacagc atcgttttag atacaagaaa taccttagat tacacaaaaa accacttaag
     4801 ccactcgctt tctatccccg ctgctatctt gccaagacaa ttaaaaaatc tagatccaaa
     4861 gaaactttat tatattctaa gctatacagg caggcgatca gaagcattag ccgatcaact
     4921 tactgctaat ggttttcgtg ctgtttatat cattggtggg atggaagctc ttcagtcgag
     4981 tgttgcttga atattgcttg ttagattcaa aagaggctgt gacaaaaaat cgtgtcacag
     5041 cctcttttat accctgtctt tttttagtta tttattcttc aaccttatca atatgacttg
     5101 atagcctttt ctttttctca tagttataac aatcatagca ataaaaggag aaggtaagca
     5161 tatggcatct gttttagtag tcaaaggtca tcccctaact gcagaagaat cgcgcacagt
     5221 taaggcattg acgtcttttt taacaagtta taaagaaaat catccagatg atgaagtgac
     5281 tgttttggaa ctttatcgcg atgatattcc ggaaatcgat gaagaattat tatcaggttg
     5341 ggaagcatta cgtgctggtg cagaattcac ttccttatca gaaagtcaac aacaaaaaat
     5401 tgctcgtttc aatgaattga ctgatcagtt ccttgctgct gataaaatcg ttatcgcaaa
     5461 cgccttatgg aatctaaata tcccaacaaa attaaaagct tggttcgata ccgtcaatgt
     5521 agctggaaaa acgttccgct atacagaaaa cgggccagaa ggattagtga caggtaaaaa
     5581 agcactgcac atccaatcta atggcggtgt atacaacgga caagactttg cctctcaata
     5641 tgtaaaaggt attttaaact ttgtaggcat cgatcaagta gatcagctat tcatcgaagg
     5701 aatcgattat gaccctgacc gtgcagatga gttgatgcaa aatgcattag ataaagctgc
     5761 tgctttaggt aaatcatttt aaaaattgag ataaatgcat acaaaagaga cagaggaaaa
     5821 aatcttcaaa gcagcatttg taccttggat attttcccat ctgtctcttt ttgttattct
     5881 ttctgctttt tcaacggatt ttcttcataa agagaagaga ttgttgtgta ccattcaaaa
     5941 acatgagtaa tcactacttt agctgctgca taaacaggaa tccccaagat cacaccgatg
     6001 attccgaaca atttcccaga agtcagcaga actaataaaa tcgttactgg atggattgct
     6061 aactgattcc ctagtactaa tggagagatc aagcgtcctt ctattgtctg ctctactgca
     6121 aaaacgatca gtactttgat gatcatgaat ggacctgcta caatggctaa aaagatagcc
     6181 ggaatcatcg ctaaaaatga tcctaagtaa gggatcaggt tcaaaaaacc cgcaataatc
     6241 cccaacgtga cagcatactc taaaccaatg accgcaaaac caatcataaa catgattgcc
     6301 acagcaaaag ccacagttag ttgtcctctt atataagaag aaacttgact attcatttca
     6361 ctcagcacat tcaacgtcgg tttttgcatg cgtgtcggta aaaatttgac aaaataaggt
     6421 gctaattgtt taccgtcttt caatagataa aataagataa acggcatcgt cagcagagcc
     6481 actaaaatag tcgctaccgc gccgacaaaa cttccaagac tctgaactgt actcttagat
     6541 atatcttgga tcatatcgcc tgccgaattc atgaacttat cacctgctgt ttccaactgt
     6601 tcgcgaaatt ggttgaacag cgggtcccgc aaaatctcgg ttaatttatt atcaatcgta
     6661 tctacatatt gagggaagtt actgatgaag ctcactgttt gttcctgaat cttagggatg
     6721 atcactacgc tcccccatgc gattagtgca acaaccaaaa caaacagccc aatgatactg
     6781 taaagccttg gtacttttcg cttttcaaag taatcaacta ccggattcat caagtaatac
     6841 aagattcctg ctaaaatgat aggcagacct acgattgcta aaaattgcca gattggtgca
     6901 aacaaatatg aaacttttgt aaataaaaag acgatcagca atattaacaa tacaacaagt
     6961 aaggctgtga ctacttgatt atttaaaaac cagcgccaaa accacgatac cggtctgttc
     7021 ttcttatttt ttccttccat aattcctcct gttagttata aaaaatttgg cttcccaaac
     7081 caatagcagg cagtctatgc ggatgaagat agattccatt tgcaatttgt ccttcttctg
     7141 gacaagcatc aaaaatcaat gatacgtgag ctactgtctc taaattccca ttggccatcg
     7201 gaccgacata atcgatcgta tatacctgat catcaaaagc aattgttccg cctgctttca
     7261 aagaaaaagg ttcgtctttt gatacttctt gaatcaccga atattttcgt aaggcagctg
     7321 ttgctgtttt atcgaacaaa atcagcatgg gttcttcctt gtttattgct tctgtcccaa
     7381 tttctgtaat ctttgcctga atcattgcac tctctccttt aattgctttc aaaaacagta
     7441 taacacattc tcattcttta gccgtgtgga ataattatgt gatagaataa aaacgaaaac
     7501 tatttcatta caattaggag gaatcagctc atatgatcga aaagataatt ttaggaacct
     7561 atacgcgtcg cgaaagtaaa ggaatctata cgatcgacct tgatacagaa aaagaagaat
     7621 tagccaacct ttcattagct gttgaagaaa atagccctac ctatgtagca aaaagtaaag
     7681 ctggcaatct ttacacagtt actagtgtgg atggattagg tggtgccggt gcgtacgata
     7741 aggattttca ttttttgaat gcggtaacag agtctggcgc tcctttgtgt tatgtagcgg
     7801 ttgatgaagc acgtcaatta ctatacgggg cgaactatca taaaggtgaa gtaaatgtct
     7861 accatatatt aggagatggc gaactgaaag cagcagattc aatattccat caagaagcga
     7921 caggtcctca taaaaaccaa gatcatgcgc atgtccacta tacggatctg actcctgacc
     7981 aacgattagt cgtatgtgat cttggtacag atcgtgtgta tacttatgat gtgtccgaaa
     8041 acggcaaatt gaatcttgta actaccttca ctgcagagcc aggaacaggc cctcgccatc
     8101 ttgtctttca tccaaatgga caattcgctt atttatttgg tgaattagac agcacagtcc
     8161 gcgtgctttc ttataataaa gaaacaggtg cttttgaaga attacagaaa atatcgacct
     8221 tgccagaaga attcgacggt gaaaatggag gggcagctat ccgcatttca aatgatggca
     8281 agttccttta tgcttctaac cgaggacata actcgattgc tgtattcgca gtttttgaag
     8341 atggttccca cattcaaaac aagcaaatca tctcaacaga aggggatttt ccccgcgatt
     8401 tcgcactaga tccaagcaat aattttgtcg tgtgtgccaa tcagaacacc gataatctga
     8461 cattatatcg ccgtcatcct gaaacaggat tactggaatt actgaaaaaa gatgtctatg
     8521 taccagaatg tgtctgtgtt tattttgatc gttagtaaga aaaggagaat gaaatgaatc
     8581 agcaagacgt attagacaga ctacgggaag aactaaatat tcctttcttt gatgggaaaa
     8641 tcgaagataa agagtatagc gaagaggaat atcagaaact caaagccgat ttacagaact
     8701 actttgaaca atatgtacag aatgtagaaa actaatcagc tttccatact atctgaaaag
     8761 aacatcagga caagactttt ccgtctatcc tgatgttctt ttctacaaat aaaaaaccgc
     8821 cgagatggtt agactcgacg gaaaaggagt atttactcga ttttgagtaa gtacaaggtt
     8881 gttgttggtt ataggtatgt cattatcata aaggattttg atcactctat cataaaggta
     8941 atattacatt tatattagat aactctagtt tttattgtta tcaaagattt cttttatttt
     9001 ttcaacaaca aattagtcat tttttggatg gcagctgtca attggtctgt cgcttggtct
     9061 tgttttgctt cagatgtatt gaatagatct tcatctaccc ctgattcaag atcgatactt
     9121 tggatcattt cttcacagcc tgctacatat gaaagataag ctttttcaaa atttttatga
     9181 atgcccattg cttttgctgg tggacgcaaa gtactgactt tcttaagcat tgcccgatat
     9241 ttttttgttc cttcttcaaa taacaggatg atctcttcaa gagtcgattt gtccagttca
     9301 cttgtttttc cttcatcaat tgcgtttctg atcacttcat atttcggatg cattccttct
     9361 ccgattgctt ctgtttcctt gacgatttga tcgatggact gcacataata gccaatattc
     9421 ggtaacatgt tcgttttcct actttctcca ttgttgttat tatttattct acttgttttt
     9481 atcctcaaat gacaagtact tgaaaaaatt taatggtttc tatacagctc gccattctta
     9541 ttcgtgctca aagaaaccat ctgctaaact atattgtttc cctgcgacaa tgtcatattg
     9601 gatcaattga tattcgtcgt aaatctcttg ttgcgagcga ttgaattgcg cttctttata
     9661 ccactgtccg ttttgatagt acagttctcg ttcaaacgcc ggaagttcct gttctaatga
     9721 taaaagcagc tgataaaagc catttgttgt ctgattcgtc tgttccatta gattcgctgc
     9781 aaaataaaac ggactggtga ttgcatcgtg agtcgtttgc ttttctaatt ctcctttact
     9841 gtcgaacata agaaattgtg tttcatgcaa tgtttgtttg tcattttttg cttggatcgt
     9901 atctgaatag atccctggaa gatgatcccc ccaaaaaaca actaaagtcc ttctagaaag
     9961 attcttcaat tcttccgtga actgcttcag tgccgtgctg ctataggaaa tatcttgcag
    10021 ataattctct aagtcaagtg tcccacttcc atctgaagtt tttgcaggat agtctagatt
    10081 atcatacttt ccatcgtagg gcatatgtgt ctgcatcgtg actaaatgaa tgaactgagg
    10141 tgttttttct tccttgagaa gtgtcattac ttctttgtaa gctgactcat cagaaatata
    10201 tggattattc tctatcgtat ccgtataggt catcgtacgc tcgctgatga actggtcaaa
    10261 tcctaatgtc tgataaacat cctctctttt atacatcgaa gtattgtatg gatggatcgc
    10321 tgtcgtctgg tatgattgtg cgtttaacgc tgaaacgata gaaggaagct gatccatttt
    10381 tggtacaagc atcgtgtaag gggtggtgag ttgcgcatta aataacgcca tagaaaaacc
    10441 agtcaatgct tcaaattcga tatttgctgt tccaccgcca tagttttgag aaagcatatt
    10501 gccactatat gtttgatcag ctacttcata ataatctgcc aatggctcac cagatacttc
    10561 tacgccgttt aatcgactag gatctgagaa actttcactc atcacgaaca cgatgttcgg
    10621 cgactcttcc tctacagttt tatttttttc atcggctagc ttctggtatt tttcagtgat
    10681 ttctttgatc ttttctttcg aatatccttc aggttcatcc attggttcta ccttcagatt
    10741 gtacaaaaac ccaccgataa aaccagtgtt atagtagttc attttctggc tgtaaggtat
    10801 ccatagtgcc gttttattat atgcttttcg caacaaatta tcgggattgt tgaaattact
    10861 gatataccct agcagtccaa ttgccacaag cagtgtcgtt aatcggatca attggaatcg
    10921 ccttcctttt ttcaggcttc gaaacatgta ccagcaaaac aatcctatca cggttcctgc
    10981 ggcagctaag atcactgtaa acagcatagt gccggtcata tctttcagca atccgatttc
    11041 agtgatcatc ttcaaatcgt ctggataaat cggttcttgc cgataactca ttttcatata
    11101 atttgcaaaa cctaatacac cgatggctac gacatacgta agggaaccta aagggacaga
    11161 tcctgctagt gcaatcagaa aaataagaat aatcagcaag acgagacagg ctaagaagaa
    11221 tttttccgta tgccaagaaa aagcaaattt caacgcaagg tctacggata gatcattttg
    11281 acaccactgc aaaaataaat tactaaaaac aacaagaaaa aagatactga tgattgctgc
    11341 tgttcctttt aaaatctttt tctgtttggt cgaaagtctg cttatatatc ttttcaaatg
    11401 cttgctcctt catttctatc aataaaagaa tgttgcttct cttattttag ttgtacgaaa
    11461 ttcgaaagta aagaacggaa agaagaatta atgaaatcca aatattctct ttctttatgg
    11521 cctttctggt ttctttaata agcggacctt tcgtactcca gaaatttttt ctagctgttg
    11581 tttcacttga ttgagctgtt caaaatcagt actgtctatg tcaatcaata tgtagacatg
    11641 ctcattctta tggttacgcg atatatctgc gatcgctagc tcgttctcat tcagcagaga
    11701 aaatatctct gctaatatat tggttttctt ttgataaaat atagtgaatc gaaacggcgc
    11761 ttggaaaaac attcgtgcgg atggataatt gaccgcttct ctaaccgtac cgaacaaaag
    11821 ataatctttt aaggcttcaa ctgccaaacg tccgctgtct gccaaagctt cctgagtcga
    11881 tccccctaga tgaggcaaca taagtatacg gtcattttct aagaactctt cttctggaaa
    11941 atcagtcaag tatttagcca gatgttgttt ttccaatgct tgcataacag catatttatc
    12001 aacaatttca cttcttccta cattgataag tacagcatct tttttcattt tttctatatt
    12061 cgcctgatcg atcaatcctt ttgtatcttc tgtcaacggc agcatgacta cgatgtaatc
    12121 cgaggtagaa agaactgttt ctaaatctgc ttgccgaaca taatccaact gggcatcgcg
    12181 gatagaataa cccaagacat ccatccctaa gctatagcaa cttagcgcta cttttgtgcc
    12241 tatcgctcca agaccaagaa gaccgatggt tttgccttgc aattctcgtc ccacataagc
    12301 acttcttttg ttttctgcct gttctaaaat attaggacct gttagtgttt gtaccatacg
    12361 acttgcttcg attatcggac gggaagataa gagcaaacag cacaaaacaa gctcttttac
    12421 tgcattagca tttacacctg gtgtattcat gacgatcgtc ccgttttcag aagctttttc
    12481 aacgttgatc gtgttcaccc caacaccagc tcttgaaatt gctagcaaat ctggtgtaag
    12541 ccattcatct ttcactttcg tactacgaat aactattcct tcggctggca aatcatctat
    12601 tattttgaaa tattcccctt gtaacttttc tatctcttgt tctggaatag catcaatcaa
    12661 atgaacgtga tacataaaac cccctcctta ctaacaagta tctcgaaatt tctgcaatta
    12721 gacaactgtt taccatttga cttcctagaa gcaaaaagag cagttagagc agagtaggct
    12781 gagtgataaa aataaggcta tgacaagtta ctgccataag ccttattttc gagagcaaaa
    12841 tattgagggt gagatatctt gataaatggt gttccgttag ctgacattga taataagact
    12901 aaatttcaaa aaagttgaaa agcaaatttc gtcaatctct tcttatttct taaaactaag
    12961 tggctttttc acaacctctt tttatctaat aaccactgtt tccaactcaa ctaattttgc
    13021 ccacgtttca atttgatctg ttgttagatt tagcgagaca acggtatgat gaccgccacc
    13081 tgcctgaatc catgaatgga caccttcatg gaaattcggt ttagttttcc ataacacacg
    13141 tgcaacaggt aaattaggtg cttcagtcgt tggttcaaag gcttctactt cattgatcag
    13201 cagtttataa tgcgtaccga aatctgccat cgatacgact accccttctc ctgctttccc
    13261 atcaaataca agacgcgccg gatcttctct atctcccatt gataaaggag aaacaacgat
    13321 tcttggtttc gtcgcagcaa gcgtcggatc tacttccatc atatgggatt ccaagattgc
    13381 ttcttggcca ctagctagct cgtaagtata atcttccatg aatcctgtat ctttccctcg
    13441 agccatgatt ttcagcaacc gatcaatcgc tgcagttttc cagtcgcctt ccccagcaaa
    13501 accgtatcct tctgccatca aacgttgtac agctagtcca ggtagttgtt tcattccata
    13561 caggtcttca aaattagtcg taaaagcgtt atagcctcct tcttctaaga aacggcgaat
    13621 accgatttcc tgctgagctt gtacttttac atgttcctcc catgtctttt cttcataatc
    13681 accatagtcg aaggtataca attccttgta ctcttcaaat aattgatcga cttcttcgtc
    13741 tgatacgcga tcgattactt gtacaagatc tccgatacca aaataatcaa cagtccaacc
    13801 aaattgaatc tgtgcttcca ctttgtctcc ttctgtgaca cccacgtttc tcatgttgtc
    13861 accaaaacga gccactttta ttccaaaact ttcattatag gctactgcaa catccatcca
    13921 atcagcaatc tctttttgga tctcaggtcg tttccagtag ccaacgacga ttttattttg
    13981 tttatttaat cgagcattga tgaacccata ttcgcggtca ccatgagcgg attgatttaa
    14041 attcatgaaa tccatatcga tcgttttcca aggaatgctt tcgttatatt gcgtagctaa
    14101 atgaagcaat ggtttttgta gaagctttgt cccgcggatc cacatttttg ctggtgagaa
    14161 agtatgcatc caagtgatca cacctgctac ttcttctcga taattgacct ctttcatgat
    14221 ttttgtgatc gtatcagcag taactgctaa ttcttgaagg cggattggat aaggcaattg
    14281 gccattttca ttcagttcat cgaccatttc ttgtgcatgt ttctttactt ctcgcagtgc
    14341 ttcttcccca tataaatgct gtgatcctac aacaaaccaa aattcttttt ctccaatatt
    14401 caacataatt agccactcct tttatttgtt ttgtccgtaa tacgcatttt ttccgtgttt
    14461 tcgtaaataa tgtttatcta aaatccgttg aggcagctct tctgaaaatg aattgagctg
    14521 tctggtaaat aaattcatct tgcatacttc atctagtacg actgcgttca ttaccgcact
    14581 ttgcgcatct ttcccccaag taaatggacc gtgtccgtgt aaaagtaccc ctggaattgc
    14641 taagggatca attttgcgtt ctttgaatgt ttctatgata acttttcctg tttcatattc
    14701 gtacccgctg tcgatttcct gttgtgtcaa aaatcgagca caaggtaccg atccataaaa
    14761 agtatctgca tgcgtcgtcc ccatcgcagg aacatccaat cctgcttgtg cccagatcgt
    14821 cgcccatgtc gagtgagtat ggacgattcc tccgatttga ggaaatgaac gatataaaac
    14881 agcatgagta ggcgtatccg aagagggatt gagtttacct tcaatgatat gattgtccaa
    14941 gtccacaacg accatgtcgt ctgctgtcat ctcttcataa ctgactccgc taggtttgat
    15001 aacgaaatat cgtgtttcag gatcaaaagc gctcacattt ccccatgtat atttgacgag
    15061 tccgtgttta ggcaagtcaa gatttgcttg gtatacttct tctttcagtt gttccagcat
    15121 tttctatcac tcctttttct tgcaattgat gaatcgctgt tttttcaatg tcgatcccct
    15181 cacggtaacg tctcataaat atctgatagc ctgctatttc ttctgctgaa ggtgtgatgg
    15241 tgattccttg gtcttgtcca aacacgtctt ctgccaagaa gttttctaaa gacttcccat
    15301 gtcgatccat catataggct gctaataacg cgattcccca cgctccacct tctccggctg
    15361 tgtccatcac tgtaactggt gcctccatag cagatgctag aatccgctgt ccaacttctg
    15421 gtgttttaaa aatcccccca tgccccacta aacgatcaat ttggacattc tcagatttca
    15481 atatatccat tccgattctc attgcaccaa aagcactggc taaatggatc cgcatgaaat
    15541 tcgctaaatc aaaacggcta cctggtgtac gtacaaataa tggccttcct tcatttactc
    15601 ctgtaatgtt ttcaccggaa aaataaccat aagaaagtaa tcccccgcaa tccgtatcgc
    15661 cttctagcgc tttgttgaat aataccgtga ataatttatc tctgcttatc tccataccca
    15721 aagaattggt aaattcttca aaaatactga cccaagcatt gatatccgaa gagcagttat
    15781 tcgtatgaac catgcctact agttctcctg aaggtgtagt gaccatgtcg atttccggat
    15841 ggacattttc tagttctttt tctaatacga tcatagcgaa ggcggaagta ccagccgaaa
    15901 tgttccctgt ccgtttggct acactgttcg tagcgaccat accggttcct gcatcccctt
    15961 ctggcggaca aaccgggatt cctggtctca aatttcccgt agggtcgatc agataagccc
    16021 ctgattcgct taacttgcca gcttcttctc ctgctactag aactttaggc aaaatgtgat
    16081 ccaagtcaat cgctacgtgc gctttttcga ttaactgacc aaaaatattt ttcatttctt
    16141 gatggtaggt tttcgttgaa ggatcgatag gaaacatgcc tgatgcatct cctacgccta
    16201 gtactttctc accagtaagc tgccaatgga cataacctgc aagcgttgtc aaaaaattaa
    16261 tttctttcac atgttcttct ttattcaaaa tagattgata caaatgagcg atactccatc
    16321 tctgcggaat attgtattga aatttttccg ttaatattcg ttctgcttct tcagtgattg
    16381 tatttcgcca agtacgaaat ggtactagta actgttcctt gctatcaaat gctagataac
    16441 cgtgcatcat tgcactgaac ccaatcgacc cgattgtcgt aagattctcg ccatagattt
    16501 cgaatacttc tgctgccatt tttcggtaac tgacgcgcag tcctttccaa atatcttcta
    16561 atgaataggt ccagatacca ttttctagtt tgttttccca ctcataacta ccagcggcaa
    16621 taggagtaaa agatgaatca atcaatacag ctttgatcct agttgatcca aactcaatcc
    16681 ctaaagatgt acgaccatct atgacatcta acatactttg cgcatttact tccttcataa
    16741 tgatttaact ccctccttct cgtcaaacct gatcaaaccg tctttttagt agcgcttaca
    16801 atgattattg tatacttttg tacgtacatg tcaagcggtt atttacaaga atacaataaa
    16861 aaacacttgt tttgtacgta cataagttat aaaatgaaat tgcttacaaa atctgatcaa
    16921 ccgccttctc gaaattctag aaggagagaa tacttatgaa aaaagaaaaa aaagtttcct
    16981 catcgtttat ttattttttt ggcgcatttg gcggcattct atttggatat gatattggtg
    17041 ttatgaccgg cgccttacct tttttacagc atgattgggg tttggcaggt aaagcttctt
    17101 tgataggatg gatcacgtct tcggtcatgt taggtgcgat ccttggcggc tctttatctg
    17161 gtcttctttc tgataagctt ggacgacgaa aaatgattct tctttctgca cttattttta
    17221 tggctggttc tgtcctttct gcatctgccc ctcacaatgg ttcttatttt ctgattgcag
    17281 caaggattct tctaggttta gctgttggcg ctgcatctgc cctagttcct gcttatatgt
    17341 ctgaaatggc tcctgctaga ctaaggggaa gattgtcagg gatcaatcaa gtgatgattg
    17401 cttctggcat gctcctttcc tatgttgcag attatttgct aaaagtgttg cctgaaacga
    17461 tggcttggcg cgtgatgtta gggctcgctg ctgtccccgc gctgatttta tttttcggtg
    17521 ttttagctct accagagtca ccgcgttttc ttatgcaatc tggccgcttg gaagaggcaa
    17581 aaagagtatt gaactatatt cggacaccaa aagaagctga acaggaattc gaacaaatcc
    17641 agctgaatgt caaacaagaa aagacaaccg gaacttcttg gcatactttg tttttagaaa
    17701 aatatcgttc cttagtcatt gccggtattg gtgtagcagt attccagcaa tttcaaggag
    17761 ctaatgctat tttttactat attccgctga tcgtagaaaa agcgactggg catgcagcga
    17821 gcgatgcatt gatgtggccg atcatccaag gaatcatttt agttgctggt tctttgctct
    17881 ttttggttat tgcagacaag ttcaatcggc gaactttatt gaaaataggc ggtagcgtga
    17941 tgggactttc attcatccta ccagctgttt taggaactgt actggatgct catacaaatt
    18001 cgttgttgat tctcttgttt ttatgtattt atgtcgcatt ttattcttgc acatgggctc
    18061 cactgacatg ggtaatcata ggcgaaatat tcccactagc tgtccgcggg cgagcatctg
    18121 gcctagcctc ttcttttaat tggattggtt ctttcctagt tggcttgctt tttcctgtga
    18181 tgactgcttc tatgtctcaa gaaatcgttt tcggcatttt tgggatcatc tgctttttag
    18241 gggttttatt tattcaagag atcgtaccag aaactcgtgg gaaatcttta gaggaaatcg
    18301 aacaaagtgc atctaaaaaa acttatccta aacgtatttc tatccatcat tcttaaagag
    18361 gttgtgacag aagtggtcag cttcaagaaa taagaaggaa ccgcttagtt tccagtcaca
    18421 aggaacaatt tcaaaaaaca gcttctcatg tttgatgaat attgtgactt gtgaccagtt
    18481 ggtaggagaa caccgtttag tggagaggtg gtgaaagaaa cgttcagcct tgagcgatga
    18541 caaaagctct cacaaacatt gcgacgagta accgtaggag caataagagg cggtaaaaac
    18601 ccgtctctta tttttcgttt tctttcactg tttcccgttc aactagtaca ggttcataga
    18661 gaatatttgg ctgtgcctgt cctttttcaa tcgttgtgac gatccaccgt gcggcatctt
    18721 gtcccaactt ttcttttgga tgcgtcaatg ttgtcaattt tttattccct aaatggctta
    18781 aagaagaatc gtctgttcct acgatcgata ggtcatctgg aatatgatag ccattttctg
    18841 tcaacttatc tagcattaaa ctagctattt gatcgttata gcaaatgatg ccatctaaag
    18901 cttgttcttc tttcaaccgt tgtactgttt tttcagcgat tgtttctcgt gtttccgtgg
    18961 tgaaagtaat gatatcttcc gtcaaaaaac gcacattata ctgttcacac gcgctgataa
    19021 aacctttcat cctgtatttt ccttgaagat cgtcgatttt tgtgatgaac aacagacgct
    19081 gatgtccttt ttttatcaaa tattctgctg ccataaaacc gactttcaca tcatctaaac
    19141 aaatataaga ggcatttaac tcttcataag cagcattgat catcaccatc ggaatgcctt
    19201 gttctctcaa agcaacataa agtgctaagt ttgggttata ctgattactt ttcgtaggct
    19261 caacgatcaa accatctacc ccataagcaa tcattttctt caaacactct ttctccaatt
    19321 cgtgatcgtt attcgtactg gctaaaagca atgaataccc agattctcgt aattcatttt
    19381 cgattcctcg gataatcgac ggaaaaatat aatcagagag atatgtggtg atgacaccga
    19441 tcgtttttgt tctgttcaat ggtgtttttt ctttcttata gctgtcatct acatatgtgc
    19501 ccgacccttt ttctttacgc agatagccct catttaccaa cacagcgatt gcttgccgaa
    19561 tcgtatgtct gctgacgtca tatatttctt gcagcttgaa ttctgaggga atggttgtat
    19621 ttacttcgta ttcctcacga aggatctttt gttttatctc ttctgcaata tcttgatatt
    19681 ttgccatttg ccttgcccct ttttatttgt acgtacatat atcataacga gtctgcaagg
    19741 tttattcaag caaaaatcaa ggtttcttgt gctcataagg catacggaac aggttcacca
    19801 aattctggaa atccttgctc attccacgta aagcattgcg cgttcgcgtg ccgattagga
    19861 ttgtctaacg gatccccttt ttggttcttc tctggtcgag catgatagat caaaatatca
    19921 aagcacccgt cttctgaaac cgtaaacgag ttgtgaccag gaccaaattg cttatttttt
    19981 tcagaagaaa cgaatactgg ctccgataat ttatgccatg attcttttaa taaaagattc
    20041 ttgtgctcat cagcccacag caggcccatg gcatagtttt catctgttgc actaccagaa
    20101 tacgtaatga atattttccc attgcgagta aggactgctg gaccttcatt tactgaaaaa
    20161 ccccgttttt cccattcata ctcaggtata gaaaggagga tctgcttccc ctttaacgtc
    20221 caaggattat ccatttcgga tagataaaga ttagaattcc cagtaatttc aggatcttgt
    20281 tgggcccaca cataatatag ctttccttga gaagagaaag ttgttccgtc taaactgaaa
    20341 gtttcccttt tcgtttttat ttgtcccttt tctatccaat ctgtattcat tggatctata
    20401 tgctgatttt caagtacgaa catgcggtga tgatgagatt catcacgtat agttttatga
    20461 ttgcttgcag caaagtaaat ataccatttt ccattgatat aatggacttc aggtgcccag
    20521 atcaattcac tcatcggtcc ttcttcatga gcctgccata caatcaggct tgcagcatgt
    20581 tctaattcat tcagtgactt tgcccttctt aattcaatca cttggtagcc tggaaccgat
    20641 cctgtaaaat aataataacc atctgtatgt ttatataccc aaggatctgc ccgttgccga
    20701 acgataggat tattataaag tgtcttcatc ataacaaatt tcctcacatt ttctttattc
    20761 taattacatt ccaagagtat ttttctatcc ctacatttaa ttcgtcatgt ttttctattg
    20821 aaaaatagtt tatttcaggt tttatttttt ctggttcttc acgtgtattt ttgtccaaca
    20881 gatcgctact ggttaatgtg tagtgctctc cttgatctag caaattgaaa tcatgttcaa
    20941 tcgttatctt ttgttgttca tttgtgcgat tgatcaagaa aatagtcagc tgattgccgt
    21001 tatctacgat cacactgtcc acataaggaa cattttttat cttttcacaa tcataaacag
    21061 ggcaaatgat ttgttgtttc aatacttgac ctttagcata tttcgataag tgttgcatca
    21121 cataataagt tggattcctc catgcttgtc ctttagcatc tgtcagtatt aaaggtattg
    21181 tgtttactaa caatgattga caactaatct ttacgcgatc tgcgtgtcgt aaaatagcca
    21241 gccctaatga accaaataat aatgtatctt ccatagtaaa gtaacaccaa tcgatgggac
    21301 taccgacttc aaaatcccta tgtctttttt ccggttgggc atgtacattc cattcatcaa
    21361 acgctagata cattgttttc tcacttctca aatatccttt tacataatca caagtagcaa
    21421 cgatttcttc aatttgacga tcgaatcgat aagcttttgc aaggtatgtc gctgtatcat
    21481 cacgctcatt tttaggaggt ttagtcaagt cttcttcatc atacttatca atatagttat
    21541 gcaaagctaa ataatctaca ttctcataag cttcttctaa tgcgatacga tcccattcag
    21601 ggtacgatgg taaacgtggc gtagagcttc caactaagat tgtttcaatt gaatcatcca
    21661 ctaacttcat gacttttgag gcttcatttg ctaacctacc atattcataa gcactcttag
    21721 cccccacttg ccatggaccg tctagctcat tccctaaaca ccataattta acgccaaaag
    21781 gctttttttg cccatttttt attcttaaat cactccagaa ggttcctttt ggaaaattac
    21841 aatattctac taaatttttt gcttcatcaa ttccttttgt tcctaaattc accgtgaata
    21901 tcggtttcgc ttgtacatga ctgatccatt taaaaaattc gtggagtcca aaagaatttg
    21961 gttctatacc tcgccatgct aaatcaattt tttttggtcg ttgttctttc ctacctacag
    22021 tatccaccca atcataacca gatgtaaagt ttcctccagg atagcgaatg acacctaatt
    22081 ctaattcctt tacataagag aggacatctt gtctaaaccc gtcaccatca gctgtttcat
    22141 ggcccggttc ataaatccct gtatagacga cacttcccat atgctcaaca aatgagccaa
    22201 acagccgaga actgatctct cctaccgtaa aatcctctct gatttttact ttcacttctt
    22261 cgtccaacag ctttccctac ctttcatttc ctatagtcaa gataatagat acaagtttcc
    22321 atacgccacc agcaaagata aaaataaaag gagggaaaag aaatatgagc agtacaaatc
    22381 ctaataacaa tacgcaacaa agaatatttc tcaaaaaatc aaaaaatact ttccatgcac
    22441 tttgttcaaa aatttgtaaa aatgtgatat ctttgttttc agaaatcaca tatatccagg
    22501 ataaaagaaa catggaataa ataagtaaaa ctacatatcc actaattgaa agtacgattg
    22561 tctgttcttc taataattga ttaacaagaa aataaataat gatcgttaac agactaagga
    22621 acaagttctt accaaaatgt tgctttattc cttgaaagaa aaaataaatg atatttcctg
    22681 atccctcacc tttccattgc gccattacat aaaaaacagc acatgtagca ggcacgatag
    22741 taataacagg caaacacacg attacccaga gaatacctaa taagatgtaa tcagaaacca
    22801 caatcaactg tccataaaat ttagtctcaa ctattttcat cttcctcact ctttcattcc
    22861 agacatagct gttccttgaa caaaatattt ctgtgcaaaa atataaacca ctagtaatgg
    22921 cagcattgaa ataaccgttc cagccattac aggtccataa aaagaaatgt tctgccctgt
    22981 aaataaagct aaccctgctg gtaaagttct catttttgaa ctacttgtca atatcaatgg
    23041 ataaagcaag tcattccaac tattcatcag cgtgaaaatc cctaatgata aaagagtagg
    23101 tttgctcaat ggcaacatga ttttcaaaaa aatattaaac tcacttaatc catcaatgcg
    23161 tgctgcctct tccaaagaat ctggtaaagt aataaaaaag gaacgcatca tgaagatacc
    23221 aaatgcagtt gtcatacgag gaatgatcaa acctgcataa gtatcaagca tgccaagcaa
    23281 attgacttca ataaatagag gaatcatgaa gacttggaat gggatcatca ttgtcagcaa
    23341 aacgaaatag aacaaaactg attttccttt gaaacgcata cgagcaaatg cataccccgc
    23401 aagagaatca aagaatacag aagtgatcac tgtccctcca gcaaaaatca ctgtattttt
    23461 gatgtaatct aataaaggga tcgttgtcca aaccttaata tagttatcta gggtatagct
    23521 atctgcaaat attctaggcg gaaatgagat aatgtctttt tcgtgtttaa aagaagaaat
    23581 aatcagccac aaaaaaggaa acgcaataac caacaatata actagcatac taatcgttcg
    23641 aatgattttt ttaccactaa ctgcttttcg actattcaca gtaactgtaa tcgagctttc
    23701 tggtgccata caggtttccc tcctatttca tttgtttttc tttccgtgac atgtacacat
    23761 tagtggagat agctatagtt gcaacaatga taaacaaaac cgaagacagt gctgatgcat
    23821 agcccaattc ataaggtgct gtaaatcctc gactgtaaat atattgaaca gctgtttctg
    23881 tcttaaattg tgggccacca gcggttgcaa catatacttg gtcaaacact tgcatcgaac
    23941 caatcagatt cgttaaaata caaaaaccta aggagggaac gatctgaggt agcgtaatgt
    24001 taaaaaactg ctgtcgattt gttgcaccat ccatttgagc agattcatac aaagagggag
    24061 agattccctg aatagcagtc aagagaatcg tcatagtaac accaaaattc ttccaaacag
    24121 tcatgatggc taccgtcggc atagcaaggg ttggatcgcg aaaaaattga gggatcggca
    24181 agccgacttg tgaaaacaag taaggaatcg cccctacatt cggatcaagc aacatagaaa
    24241 aaataatcgc aattgacgtc aatgaacaaa taactggaat gtaaaatgtc gaacgacaaa
    24301 aacgattgaa tctggtattt ttagataatg ctactgctgc taataaacca acgataattt
    24361 gtgttggcgt ttcgagtacg gtgaaatata aagaatgaat caacgaatta actgctcgtt
    24421 catcactaaa tatacgtgtg aaattttcaa aacttacaaa atttcttgat gtaaagaaaa
    24481 tattcaaatc tgtaaaacta atagcaaatg ttcctaaaag cggtatgaca gtaaataata
    24541 ataaaactaa aaaagatgga gttaaaaaca aatacgcagc acgatcagga cgattccaat
    24601 atttggtgaa tccgttcata tagtccacct catctttcat tttgataaaa tatacaatct
    24661 tggtttaaac gaatctgaat aagatatccg aacaatcgct gtgatttttg caataacacc
    24721 tatctgcaaa tactaggtcg atcattcaga tatctgtatc ttgccaacag tctatcctgt
    24781 ataaagttct gtactatttt tgcagtaatt gattgatggc atcatctgca tcgttcatga
    24841 gtgtctcagg atcttctcct gctaataatt tttcaatcag tggattgatg atatcgttat
    24901 tgatcgcaga gatttgggta aaacctggta aatatggctc tgcatagtcc atttgtttcg
    24961 ataattcaga tacaatagga tcatttttta cttcttcatc ttttgctact gaatccaagt
    25021 aggcaggaaa accattttcg atactccatt ttttaccaat ttcagttgta ttccaatatt
    25081 tcaaaaactc atagacagct tcttttttat tattatctgt acttgccgga atggcatatc
    25141 ctacgccatc taaaatagct gtttttttgt ctgaatcact cccttgtggt aaggtagtca
    25201 cattataatc aatctcgttt gctttcaatc cgttatttaa ccatggccca ttgatttcaa
    25261 tggctagttg accagcattc attaaattgt ccatttcggc gccagaaatc gattctggac
    25321 cagtcttgtc tacatggatc attttttgga tcgcttttaa cgttttaagg ttctcaggag
    25381 acgcaaattc agacttagta taatcatcgt tcactaattt accgccattt gctaacatcc
    25441 agttatataa cggtgcagtt ccatccttat tgaagacaaa acctgataca tttttactac
    25501 tatcagttaa ttttggtgcc atttctgtca attcttccca agtagtaggt ggttgttcag
    25561 tatctaatcc agcttgttta aacaaatcat tattccaata catatacatg ccttgtacct
    25621 gcatggggac aaaatactgt ttaccttcta cttcacctaa ttcaatagcc gtttctttaa
    25681 aatcagattt atctactcca tcatattccc agaaatcgtc taatggttgt accgctccgt
    25741 tttctacata ctgtgcaaaa tctggataat ttaatgcaat aaagtcaggt gcttttttag
    25801 aagaaatcgc tgtaggaagt ttttcattca aattggccca agtcatgata tccatttcaa
    25861 ttttgatgtt tttatcattt gttttattaa attcattaac gatttcttgc aatatttcac
    25921 catcagatgc agtaaaccca ttccaaaaag tcaaagtcac ttctttatca gaatcagatt
    25981 ctgctgaact tccgccaccg caagctccta aaatacctgt tgcagctacc aacaaagagc
    26041 caattaacca ttttttacct ttcatttttt gttcctcctt catctgataa acgacatctg
    26101 accacattcc aagatagcgg tttcaataaa caattgattt tcccatcttc agcttttaca
    26161 tttgttgaat gacttggttt tacttgttca cttttaggaa gattagttgc ttttgtatca
    26221 aatccactca tttcagaaaa atcaatcact ccctctaatg ataattcaga caagtgaaca
    26281 gttagattca tttcttcttc tccacggtta actgcaaata tgacaagttc tttgtcattt
    26341 attacggcta tactttctaa atacggcact tcttcaaaat cttccgtcgc atagtttgct
    26401 gaacgaattt gtgggcttaa cacctctcct tgtccataca cagcagcttg cataaatgga
    26461 taatagatcg tctgtctcca agcttctccg tttgtttctg tcctgattgg tgcaataacg
    26521 tttaccaatt gagcaagaca agcaatcttt actctatctg catgtttcaa taaagtgatt
    26581 aatagacagc ctaatagtaa tgcatcttca aaattatagt tatcttctag taaaggagga
    26641 gcaatctgcc aaggcgctgc ttgctcatct tgttccgctg aatgatacca aatattccat
    26701 tcatcaaagg acaggttgat ctgcttctta ctatgtttct tcgccttgac tgtatcgcac
    26761 atcgctgtca cttcagaaat gaaccgatcc atgtctaagg aacgtgcaag ataatgagaa
    26821 agatcatttt tttggttgcc ataatattga tgcagagaca aataatctat ttgatcgtac
    26881 gcttgatcaa gaactgttaa ctcccaatca ccaaatgtag gcatttgccg attcgaactt
    26941 ccgcataaaa ccacctcaat tgaatcatct acgagtttca tcgccttagc cgtttcctct
    27001 gctaaccgtc catattcttc cgctgtttta tgtccaatct gccaaggacc gtccatttca
    27061 ttccctaaac accatgtctt aattccaaaa gggctctctt gaccattcga acgccttaaa
    27121 tcactccaat aagttccttt aggaaaatta caatattcga ctaaattacg agctgcatct
    27181 actccacgag tacccagatt gacagccata ttgatttcgg cccccacatg ctgacaccag
    27241 ttataaaatt catgaatccc aacttcattc gtttcaattg tccgccaagc taaatccaac
    27301 cttctaggtc tatttgctat gggcccaata ctgtcttccc aacgaaatcc tgacacaaaa
    27361 tttccaccag gatatctaat catgggaatg ttcaattctt ttaccaagtg aatgacgtct
    27421 tggcgaaatc cacttttatt agctgatgga tgatctggct gataaatccc ttcatatact
    27481 gctcgtccca tatgttcaat aaatgaactg tacaaccgcg gatcaacttt gcctacacgc
    27541 ttgacactac taatcaacat tgtggcttcc ataactgacc tcctttaatt tatacatgaa
    27601 taatacacct cgttgaaagc gttgtcaata atataaatgt taaacttact tattttttat
    27661 cgattttaat taaccaaaat attaataatg attaaaacaa ataatttaat ataactttta
    27721 ttaacaaata tgttaatata taagaaaaac cttttaagag gtgatatata tgcaactaca
    27781 aataaacgaa gaatccttac cggtatatga agcattagct agtaaaacaa ggatcaagat
    27841 catccagcta ctttccaaaa aaaagatgaa tgtcaaagat ttggcaaaag aacttggagt
    27901 gagcagtgct atcacgacga tgcacgtgaa aaagctagag gaagcaaaca tcatcaaaac
    27961 agaaaaagta ggacaacaaa aaatttcgag ccttcgtgtc gataaaatcg atattagttt
    28021 tccagaaaaa atattcaatg cttttgatac gaaagaaaca tcgataccta ttggtcacta
    28081 tacaaattat gcaattgaac caacatgcgg tttagctacg atccatgatt ttatcgggaa
    28141 agtggatgaa ccacgctatt ttatggatcc tcgaaggatg gatgcccgca ttctctggtt
    28201 taccagcgga tttgtcgagt atcaggcacc taatttttta aatacagagg atacattgga
    28261 gatgttggaa gtttctgtgg aaatttcttc tgaatttcct ttttcaaatg acaattggcc
    28321 ttcagatatc actttcagcc taaatggtgt ggagctagga acatggacga gccctggaga
    28381 ttttgctgat atccgcggta aatatacgcc tgattggtat ccagataatc ttaatcaata
    28441 cggcttgctg aagacgatcc gaattaccaa acacttgacg aatatgaatg gagaaccctt
    28501 gtcaaacatc acgatcaacg acatccctaa agagcaagac acttggcatt tgcgaatcga
    28561 agtgaaagac gatgccaaac atgtgggggg ctgtacttta tttggtaaag gattcggtaa
    28621 ttacgatcag gatatcaaat taaaagttta ctacagctga tatttttctt ttctatttta
    28681 taaaacaaaa acagccaatc cctatgaagg gacggctgtt tttgctttat tgttatttcc
    28741 agaaatcatc caaaacggaa attggcaaat gtcgtttatg ttgtgtcttg ttccaccatg
    28801 cttcgatttt ttcttgtgct tgagcgggaa cttcttttcc ttctagataa tcatcaattt
    28861 cattatatgt gacacctagt gcgacttcat ctgcaatcat tggtttccca tcttcaagat
    28921 ctgcagtcgg aatttttgtg tataattttt ccggtgcatt caatgcttga agcaattgtt
    28981 tcccttgtcg tttgtccaaa cggaataatg gcaaaatgtc ggctccacca tcaccaaatt
    29041 tcgtgaagaa tccagtaatg ttttctgcag catgatctgt ccctaatacg gctcctgctc
    29101 tttctccagc tacggcatat tgcgtaatca tacgttgacg tgctttgata tttcctttgt
    29161 taaaatcgct gattcccaca cctgcttcgc ttaatacttg tacttgtgca tcaacagctg
    29221 gtttgatatt tactcgcaga ctaacatcgg gctggataaa gtcaagagca gcttttgcat
    29281 cttcttcatc tgcttgttca ccataaggaa gacgaacagc gataaattga tagttttcat
    29341 cttttgtttc ttcacgcatt tcttccatcg ttagttgcgc caaacggcct gctaaagtag
    29401 aatcttggcc gccgctgata cctagtacaa aagttttaag aaacggatat tttttcaaat
    29461 atgctttcat aaagtcaata cttttacgta tttcttcttt tggatcaatt gttggtttga
    29521 cacctaattc attgataatt tctgtctgca ggctcattta ttctcctcct atgcttctgt
    29581 atgaagttct gctacgcttt ttttcattcg ttccatgata gccattttat ggttccagca
    29641 ttcagtagac agatcgactg ggtatttttg cggattcaag tctcgtttat actcttccca
    29701 tagtgaatct agattttctt ttgtatattc tttgatttcg tttagtgcag gtaattcgta
    29761 aactcttttt ccttcgataa agatatcttg taaaactggg cgagctgtaa agtctcgaac
    29821 agttttattg ataaacgtat gcactggatg gaacatgaag atagcttctt cttctcgagg
    29881 gtcttcatcc cataatgtca cgtagtctcc ttcagatttc ccatcaaaat ttcgagtgat
    29941 gcgccacact tgttttttcc ctggagtagt tactttttct gcattgcttg ataacttgat
    30001 cgtatccatc atttttcctt catcatcttc gatcgatact agcttgaata ctgcaccaag
    30061 ggctggctga tcataggctg tgatcaattt tgtcccaaca ccccatacat caattttagc
    30121 tttttgcatc tttaaattca aaatcgttgc ttcatccaag tcattcgaag catagatttt
    30181 tgcttccgta aatccggctg catctaattg ttcccgaaca cgcttagata tataggccat
    30241 atctccactg tcgatgcgta cgccttggaa gttgattttg tcacctaatt ctttcgccac
    30301 gcgaatcgca cttggcacac ctgatttcaa cgtatcataa gtgtcgacta agaacacgca
    30361 gtctttatgg gttttagcgt aggccataaa tgcttcataa tcatttccat aagattggac
    30421 taacgaatga gcatgggtcc cgctagctgg tatcccaaat atcttcccag cacgtacatt
    30481 gcttgttgca tctgctccgc cgatatatgc cgctctagtt ccccagactg ctgcatccag
    30541 ttcttgtgcc cgtctagttc caaattccaa tagtggatct tctccgatga cagatttgat
    30601 acgagctgct tttgtcgcga tcaacgtttg gaaattgacc atattcaaca gtgctgtttc
    30661 tactaattgg cagtgcgcta aaggcccttc cacttggatc aatggttcat ttgcaaaaac
    30721 tagttctcct tcacgagcag aacgaactgt tgctttaaat tcaaaatttt gtaaatactc
    30781 caaaaaacct tcaggataga cttctaatcc acgcaagtaa gcaatatcgg aatcattgaa
    30841 cgtcagattc tctaaataat tgactaaccg ttccaatcct gcaaaaaccg cgtatccgtg
    30901 gttaaaaggc atttctcgaa agtaacattc aaaaacagca tgtaaatcag ctcgacctaa
    30961 ttcccagtat gtctgcatca tattgatttg atatagatcc gtatgcagcg tcaaactgtc
    31021 gtctgggtaa gtattattca tcttgtatta ctcctcttgg caagttttca tttcctaccc
    31081 attataacaa aaaaaattaa taggaaaaga aatcttccat tttttctgaa aaagcacatg
    31141 ttttacggat atttcttgct aaaaatgaac tccatctgct aaaactatcc tttcttattg
    31201 gtatttaacc taaacgagaa agaatactta ttttattcaa tgtatccgag acacgaagaa
    31261 attagcgttt tctatttttc ttacttgata aaataagtag attttcctac tacaatcgta
    31321 gccgttaaga taccgatgat tgaacaaata taaccaaatg tctctacaac ataatcaatg
    31381 gcaaaagttt ttgaagcaat aatacgtctt tccttttttg cttcttatcc ccaaagaaac
    31441 ggatataact tattatgtat tgaatttttt tggaaaggcg aattatgaca agatatacct
    31501 tgcgttgaaa aaactcgcgg aaaaaatatc acacgttttt aaaaagatac aaagtaagaa
    31561 agcagctgtc aatagaatgg aaacaaaaat ggcgagacat caatcagtct cgccttgttt
    31621 tttattgtat acattagata tggagcaggt taccataaag atgattatta gtcgttatta
    31681 aatagaatcc ttcgtatttt tttgttctat cactacaata tctttatttg gacgtttgaa
    31741 ttgtattccg gcacgttcta gctcctgttt cactcctaca tggactttga catcttctcg
    31801 tgtcattttt aaaacgtgat gaatcgcata gtcgatttct tgccttgtga tttttttctt
    31861 cgtattgatc atcacgactt gctttttatc atatgtgtct accaatagca gttgtgttcg
    31921 atctaacgaa aataatttgt aataattaat agccgtattc ccaaaaaaga attccactac
    31981 tttaggataa aatgtcttaa tatttaaatt actattcaca ggtgtttcga ttagcttcat
    32041 ctcattcctc ttttctatat agtcgattta gatccaatct taagttatgt taccatcttt
    32101 cctagcatta aggaatcggt tacaattgaa cctttaaaga caaaaggatt taccttttat
    32161 cacttaatag tataacataa tttcatgaga aaaaaataca attttttctt gaccatctcg
    32221 ttacgtaaag gattatgctt acatttgtca ggaggaaaaa taatggagta cacaataaag
    32281 aaaatgtcag agatatcagg agtcagtccg agaaccttgc gcttttatga tgagattgga
    32341 ctgctaaaac cagctaggat caattcttca ggttatcgga tttatggcaa aaaagaagtc
    32401 gatcgtctgc aacacatctt attttatcga acaatggcat tcaagttgga tgatatccaa
    32461 gaggtcttag acaatccatc ttttgaccac cagaaagctt taatcaagca tcagcaaatg
    32521 ttgctggaaa aacgggcaca aatcgatacc ctcttgacaa ctgtccaaca gacacttgat
    32581 atgtacgaag gaggaagaaa aatgagtgac aaagaaaaat ttgaagggtt taaacaacaa
    32641 aagttgaaag aaaatgaaga atcctatgga caagagattc gacaaaaata cggaaaaaaa
    32701 aaggttacag catccaatga aaaatggatg aatatggata aagaaacgta tgaagaaatg
    32761 caaactgttg aaaagcagat gttatttgat ctcactcttt acttaaaaga accagaagat
    32821 gaaagcttag cagaccgtat cttccaagct cataagaagt ggttatctta cagctggcct
    32881 gaatatcagc caaaagcgca taaaggacta ggaatgatgt acgaagccga tgaacggttc
    32941 actgcatatt atgacgagcg aagtggtcat ggtgcagcaa aagctttaaa tgaaattatc
    33001 caaaaaaagg cgtaatcttt tacatgatcc aacaaaaaag tacacgtaaa atgacctaat
    33061 aacaaaacag cagacaccca agttagattt ttatgctaac ttttggtgtc tgctccattc
    33121 tttcacaagt tttttcatcc tactttaaat atattcatcg ttgaataaac gtataaggta
    33181 ttttcacttt atcctttttt tctgtaagaa acaattgaaa tgccatttct ccgcaagctt
    33241 ccagatggtg gtcgatggtt gggaaaccaa gaagctcgct gatcagcaaa ttatcgcgtc
    33301 caatcaccac tggtgtctta tcttgcagat agctttgcaa gatgcctgct gcaaccatat
    33361 ccgaatttgt aaagacacca tcaactccaa gctctttaaa aaagaagcct gcttgttttc
    33421 ctttctctgc atctatacaa tcccaaaaaa caagtgattc atcaaaagat ggaaaaagtt
    33481 gatgacatag ctgcaaagtg atttttgagt tataacttag cctgccactt cgccccaacg
    33541 tgatgcccaa ttgctttata ccttgctgct tcaaataagt aagaccttct gtaattgatt
    33601 gttctcgatc aataaagaca cagcttgcct gttttccctt gatttcttca caaaaaacta
    33661 gaggtccata tttttgatat gccaataaat catctagtgt attggcacga gacgtaatga
    33721 tcaatccatc gaaagcttta gctgcaaatt cttcaagata ccttcgctct ttttctttct
    33781 gatagcccgt cggtaaaagt gtcactttgt aatcataatc aaacgctgct tcaatgatcc
    33841 cactgatcaa ctgatcaaaa tacggatgat tggtataagg cagaatcact ccaatgcttt
    33901 tcgtttttcc ataacttaac tgtctggcat ttgcattagg gatataatcg acttctttca
    33961 tgaccgcaag tatttctttc cttttttcct catctacata tggatgtcca ttgattacac
    34021 gtgaaatagt agaaactgaa taacctgtga gctttgcgat atctcgaatg tttgccactg
    34081 ctgcttcgcc acctatacaa aaagattttc ttgtaaatag tctaccacag ctggaccgca
    34141 ttcaaaaaac tgatacattt ttccttcatg cgaaaaactg atccgctgcc cgccccaata
    34201 tatatctttt gtcaaatttt cgatttcatt cattggtatt tcttttacta tattcctatt
    34261 caacccttga aaactgtaca acgttaatac ggatcccttg aactcgacaa acgcataaaa
    34321 ataattgttc gttgtgccat ctgttatctc tacataagta ttcattgcaa gccctccttt
    34381 tgatactttg agtatagtgt atgaaacgcg tctcatagca aggagtttgt tttgccaaag
    34441 aaaagtttta ttcctctttc attattacca aagtctctta ttttaaattg aatacattat
    34501 aatgatactg taaatatatc tttattatta gtgaatggat atttactttt aatagataaa
    34561 atattataac tattaaaaat aactattttc attataatta cacacaaaaa agagcgtgag
    34621 acaaaagtaa agaac
//
LOCUS       DACDXE010000024        34537 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.024, whole genome shotgun sequence.
ACCESSION   DACDXE010000024 DACDXE010000000
VERSION     DACDXE010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.024"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            591..1280
                     /locus_tag="IWM23_001177"
     CDS             591..1280
                     /locus_tag="IWM23_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9883834.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="IWM23_001178"
     CDS             1267..2475
                     /locus_tag="IWM23_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9883835.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="IWM23_001179"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="IWM23_001179"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAP9883836.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM23_001180"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="IWM23_001180"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAP9883837.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="IWM23_001181"
     CDS             complement(6578..7273)
                     /locus_tag="IWM23_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAP9883838.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM23_001182"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="IWM23_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAP9883839.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="IWM23_001183"
     CDS             complement(8472..8663)
                     /locus_tag="IWM23_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAP9883840.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="IWM23_001184"
     CDS             complement(8684..9574)
                     /locus_tag="IWM23_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAP9883841.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="IWM23_001185"
     CDS             complement(9561..10328)
                     /locus_tag="IWM23_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9883842.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM23_001186"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="IWM23_001186"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAP9883843.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM23_001187"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="IWM23_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAP9883844.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM23_001188"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="IWM23_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAP9883845.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14944..15282
                     /locus_tag="IWM23_001189"
     CDS             14944..15282
                     /locus_tag="IWM23_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883846.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="IWM23_001190"
     CDS             complement(15450..16208)
                     /locus_tag="IWM23_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9883847.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="IWM23_001191"
     CDS             complement(16226..17044)
                     /locus_tag="IWM23_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9883848.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM23_001192"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="IWM23_001192"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAP9883849.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM23_001193"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="IWM23_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAP9883850.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM23_001194"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="IWM23_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAP9883851.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM23_001195"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="IWM23_001195"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAP9883852.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM23_001196"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="IWM23_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAP9883853.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="IWM23_001197"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="IWM23_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAP9883854.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM23_001198"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="IWM23_001198"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAP9883855.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM23_001199"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="IWM23_001199"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAP9883856.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="IWM23_001200"
     CDS             27086..27385
                     /locus_tag="IWM23_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAP9883857.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="IWM23_001201"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="IWM23_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9883858.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM23_001202"
     CDS             27973..28209
                     /gene="rpsR"
                     /locus_tag="IWM23_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAP9883859.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30226
                     /locus_tag="IWM23_001203"
     CDS             28352..30226
                     /locus_tag="IWM23_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HAP9883860.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDGLIG
                     ISARSTGTINVQIIMESLGGGGHFTNAATQLENTTVEETKQKLFAAIKENMNQIYDKE
                     "
     gene            30232..30684
                     /gene="rplI"
                     /locus_tag="IWM23_001204"
     CDS             30232..30684
                     /gene="rplI"
                     /locus_tag="IWM23_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAP9883861.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30958..32325
                     /gene="dnaB"
                     /locus_tag="IWM23_001205"
     CDS             30958..32325
                     /gene="dnaB"
                     /locus_tag="IWM23_001205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAP9883862.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33105..34397
                     /locus_tag="IWM23_001206"
     CDS             33105..34397
                     /locus_tag="IWM23_001206"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAP9883863.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10515 a         6716 c         6825 g        10481 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacggc ttgattggga tcagtgccag
    30061 aagtaccggc acgatcaacg tccaaatcat catggaatcc ttaggtggcg gaggacactt
    30121 cacaaacgca gctactcaac tggaaaatac gacagttgag gaaacaaaac agaagctatt
    30181 tgctgcaatc aaagaaaata tgaatcaaat atatgataag gagtaatcca aatgaaagta
    30241 atttttttac aagatgtaaa aggaaaaggt aaaaaagggg acatcaaaga agttccagca
    30301 ggttatgcac aaaattatct aatcaaaaat aacttggcaa aagaagcaac gaaaagcagt
    30361 atcagtcagt tagcaggaca aaagaaagca caagaaaaac acgaagctga aatcttggaa
    30421 gaagctaaaa agttaaaaga attcttggaa aaagaagata ctgtagtcga attgaaggcg
    30481 aaagcagggg aagacagccg tttgtttggt tcgatccctt caaaacaaat tgctgaaggg
    30541 ttaaacaaac aatacaaaat caaattagat aaacgaaaaa tcgaattaga aaatccgatc
    30601 cgttcactag gctacacaaa cgtacctgtt aagcttcatc atgaggtgac ggctacgatc
    30661 aaagttcatg tcgtacaaga ataacaaatc ttgatagcga tgttcagctg gtaagtataa
    30721 ccagaaggaa aaaagacaaa ccgaaattta tctgagaaaa cagctgcagc aatgaaagca
    30781 aaagctgcaa atcgatttca gattgatttc ggtttctttt ttgggaacag caaaatagca
    30841 taaagaaaac gagtataata aaagagaact atttttttat tacactttcg gtacgaatga
    30901 tgacttttct ctcattttct tgtaaaatga agcaattgaa gacaaggagc gatacgtatg
    30961 aatgaagtgt ggcaggatcg aatcccaccg caaaatattg aagctgaaca agcggtttta
    31021 ggctctgttt ttcttgacgc agaaacgatc attgatgcca tggagtacat agagccaaaa
    31081 gacttttatc gtcgtggcca tcaaattatt tttgagacaa tgatcgagtt aaatgatcgt
    31141 aatgaagcga ttgacgtggt tactgtcaaa gaccggctgg aacaagccaa tttgttagaa
    31201 gatgccggtg gtttaagtta tctctcagac ttggcattag cagttccgac agcagcaaat
    31261 atcgtctact atgcgaaaat cgttgaagaa aaatcattgc tgcgtacatt gatccagaca
    31321 gcaacaggaa tcgttacgaa aggattcgag caaggagaag aagttcaaac aattttagat
    31381 gatgccgaac gcagtatctt ggaggtttcg gaaaaaagaa atcgaagcgg ctttttatct
    31441 attgctgaag tactgaactc cacgattgaa aatatcgatc gactagctca aaacaatgaa
    31501 gaaatcacag gtctaccaac tggctatcaa ggtctggata aaatgacagc aggtttccag
    31561 cccgaagaac taatcattct tgctgcacgt ccagcggtag gtaaaacagc gtttgcattg
    31621 aatattgctc aaaatgtagg aacaaaaaca gatcgttctg ttgcgatatt tagtttggaa
    31681 atgggggcag aatctcttgt aaatcggatg ctttgtgcag aaggttcgat tgaggccagc
    31741 catttacgta caggtcagct ttcggaagaa gaatggtcga atctgatcgt tgcgatgggg
    31801 agtctttctc gagcaaatat ttttatcgat gatacaccag ggatcaaaat ctctgaaatc
    31861 cgtgcgaaat gtcggaaact agcgcaagaa aaaggcaatc taggcttgat attgatcgat
    31921 tatttgcagt tgattgaagg taacggtaaa gagaaccgtc aacaagaagt atcagatatc
    31981 tctcgccaac tgaaaaaact ggcgaaagag ctgaaagtac ctgtcattgc tctttcacag
    32041 ttatctcgtg gagtagaaca gcgacaagac aaacgaccag tgctaagtga tattcgtgaa
    32101 tccggatcaa ttgaacaaga tgcggacatc gtagccttct tgtatcgaga tgattattat
    32161 gaaagagaag gcggagatga cgatgatgga aacgagccaa aaaatgacaa cgtcatcgaa
    32221 gtcatcatcg agaagaaccg aagcggtgca cgaggaacag ttgaactact gtttatcaaa
    32281 gaatacaata agttttcgtc gctttcgcca agagaagaat tttaaagaag aggttgtgag
    32341 ccttgagcaa tcccgagagc tctcgtaaga agatgcaagg agtaaccgca ggaacgacta
    32401 gagtacttaa tttaaacaga tctccaaagg gattgtgaca aaagtcttgt cacagtccct
    32461 ttttccgaat aaacggtgtt caatcagctg acccttggta ttagctcagc aaacgtcaaa
    32521 atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg ctgatcttac
    32581 gacctctttt ccttaaacgg tgttctcctg ccaactcctc ggtcacaagt cacaatatca
    32641 tcaaacatga gaagcagttt tttgaaattg ctccttgtga ctcagagtta aacggcaggc
    32701 tcacaacctc tttcttaaac ggtgttcaaa agcttgcatc tgcggtaata cgcccaatca
    32761 aacgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat gcaggacagc
    32821 tttttcacaa cctctgattc acggtgttcc aaaagtagct tcttcgaaaa ataagtcgaa
    32881 atttcacaaa aatttgagaa gcaattttcg tacattcctt cttatttctt gaagctgacc
    32941 acttctgtta caacctcttt tttaataacc attctaaaaa gataaagttc gttttttaag
    33001 cgtttttata aaataaaaat aaaaatcatt cgattttatt tgaaatatgt atctattatt
    33061 gatacaatgg ttcaggatta aataatatag aacgaggtgt tcacatgtca tctgtagtag
    33121 tagtaggtac gcaatggggc gatgaaggga aaggaaaaat cacagacttt ctcagtgaaa
    33181 atgctgaagt gattgcacgg taccaaggtg gcgacaatgc agggcacaca attaagttta
    33241 acggtgtgac ttataaactg catttgattc cttctgggat tttttataaa gacaagatca
    33301 gtgtgatcgg aaacggcgtt gtagtcaatc ctaaatcatt ggttaaagaa ttagcttatt
    33361 tacatgaaaa aggtgtagaa acaagtaatt tgcgtatttc tgaccgcgcg cacgtaatct
    33421 tgccttatca tatcaaattg gaccaattac aagaagatgc taagggcgaa aataagattg
    33481 gtacaacgat taaaggaatc ggacctgcct atatggataa agcagctcgt gtaggtatcc
    33541 gtattgcaga cttattagat aaagaaattt ttgaagaacg tcttcgtttg aatctagatg
    33601 acaaaaaccg tcaatttgta aaaatgtttg attcagaacc acttgatttt gacgatattt
    33661 ttgaagaata ttatgaatac ggacaacaaa tcaaacaata cgtcacagat acttccgtta
    33721 tcttgaatga tgctttggat gcaggcaaac gcgtgttgtt tgaaggcgca caaggtgtaa
    33781 tgctggatat cgaccaggga acttatcctt ttgttacttc ttctaaccca gtagccggtg
    33841 gggtaacaat cggaagcggt gtagggcctt caaaaatcaa taaagttgtc ggcgtctgca
    33901 aagcctatac gtcacgtgtc ggtgatggac catttccaac tgaattattt gatgaaacag
    33961 gagaacaaat ccgcgaagta ggacgagaat acggtacgac aactggtcgt ccgcgtcgtg
    34021 taggctggtt tgattctgta gttatgcgcc attctaaacg agtttcaggt attaccaacc
    34081 tatctttaaa ctcgatcgat gtattaagtg gattggaaac agtaaaaatt tgtacggctt
    34141 atgaattaga tggcgaacta atctaccact atccagcaag cttgaaagag ttgaaccgct
    34201 gcaaaccaat ctatgaagaa ttgccaggct ggtcagaaga cattacagga tgcaaaacac
    34261 ttgcagaact gccagaaaac gcacgtaact atgtacgtcg tatctcagag cttgttggtg
    34321 tacgtatctc aacattttct gtcggacctg atagaacaca aacaaacatc ttagaaagcg
    34381 tctgggcgca aatctaagga taaaaacgac gactgaatca ggacgttctt accagagcga
    34441 gtcgaagtgt ttatctatac gataataata acttgaacac tgtttattca gaataaggga
    34501 ctgtgacaaa aaccgtgtca cagtcccttt ttccgaa
//
LOCUS       DACDXE010000025        33390 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.025, whole genome shotgun sequence.
ACCESSION   DACDXE010000025 DACDXE010000000
VERSION     DACDXE010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.025"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            949..1422
                     /locus_tag="IWM23_001207"
     CDS             949..1422
                     /locus_tag="IWM23_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAP9883864.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="IWM23_001208"
     CDS             1431..1658
                     /locus_tag="IWM23_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9883865.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="IWM23_001209"
     CDS             2294..2665
                     /locus_tag="IWM23_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883866.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="IWM23_001210"
     CDS             complement(2921..3163)
                     /locus_tag="IWM23_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9883867.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="IWM23_001211"
     CDS             complement(3195..4097)
                     /locus_tag="IWM23_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAP9883868.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="IWM23_001212"
     CDS             complement(4110..4298)
                     /locus_tag="IWM23_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAP9883869.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM23_001213"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="IWM23_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAP9883870.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="IWM23_001214"
     CDS             complement(4913..5806)
                     /locus_tag="IWM23_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9883871.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6821)
                     /locus_tag="IWM23_001215"
                     /pseudo
     CDS             complement(5884..6821)
                     /locus_tag="IWM23_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6855..7205)
                     /locus_tag="IWM23_001216"
     CDS             complement(6855..7205)
                     /locus_tag="IWM23_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9883872.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="IWM23_001217"
     CDS             complement(7238..8140)
                     /locus_tag="IWM23_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9883873.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="IWM23_001218"
     CDS             complement(8133..8990)
                     /locus_tag="IWM23_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAP9883874.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="IWM23_001219"
     CDS             9324..10133
                     /locus_tag="IWM23_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9883875.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="IWM23_001220"
     CDS             10173..10670
                     /locus_tag="IWM23_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9883876.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="IWM23_001221"
     tRNA            complement(11055..11128)
                     /locus_tag="IWM23_001221"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="IWM23_001222"
     CDS             11315..11638
                     /locus_tag="IWM23_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883877.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="IWM23_001223"
     CDS             11802..12056
                     /locus_tag="IWM23_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883878.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="IWM23_001224"
     CDS             12126..12371
                     /locus_tag="IWM23_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9883879.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="IWM23_001225"
     CDS             12447..12812
                     /locus_tag="IWM23_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883880.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="IWM23_001226"
     CDS             complement(12873..13436)
                     /locus_tag="IWM23_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883881.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14004..14204)
                     /locus_tag="IWM23_001227"
     CDS             complement(14004..14204)
                     /locus_tag="IWM23_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9883882.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            complement(14956..15669)
                     /locus_tag="IWM23_001228"
     CDS             complement(14956..15669)
                     /locus_tag="IWM23_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9883883.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IWM23_001229"
     CDS             complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="IWM23_001229"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9883884.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16764..17207)
                     /locus_tag="IWM23_001230"
     CDS             complement(16764..17207)
                     /locus_tag="IWM23_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9883885.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17241..17594)
                     /locus_tag="IWM23_001231"
     CDS             complement(17241..17594)
                     /locus_tag="IWM23_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883886.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17706..18107)
                     /locus_tag="IWM23_001232"
     CDS             complement(17706..18107)
                     /locus_tag="IWM23_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883887.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18144..18653)
                     /locus_tag="IWM23_001233"
     CDS             complement(18144..18653)
                     /locus_tag="IWM23_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883888.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18675..19532)
                     /locus_tag="IWM23_001234"
     CDS             complement(18675..19532)
                     /locus_tag="IWM23_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9883889.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19550..20383)
                     /locus_tag="IWM23_001235"
     CDS             complement(19550..20383)
                     /locus_tag="IWM23_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883890.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20397..21194)
                     /locus_tag="IWM23_001236"
     CDS             complement(20397..21194)
                     /locus_tag="IWM23_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9883891.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21227..21511)
                     /locus_tag="IWM23_001237"
     CDS             complement(21227..21511)
                     /locus_tag="IWM23_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9883892.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21508..22509)
                     /locus_tag="IWM23_001238"
     CDS             complement(21508..22509)
                     /locus_tag="IWM23_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9883893.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IWM23_001239"
     CDS             complement(22511..23413)
                     /gene="gnd"
                     /locus_tag="IWM23_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAP9883894.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            23577..24425
                     /locus_tag="IWM23_001240"
     CDS             23577..24425
                     /locus_tag="IWM23_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9883895.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            25071..25277
                     /locus_tag="IWM23_001241"
     CDS             25071..25277
                     /locus_tag="IWM23_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAP9883896.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(25479..26480)
                     /locus_tag="IWM23_001242"
     CDS             complement(25479..26480)
                     /locus_tag="IWM23_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9883897.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(26485..28398)
                     /locus_tag="IWM23_001243"
     CDS             complement(26485..28398)
                     /locus_tag="IWM23_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9883898.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(28566..29072)
                     /locus_tag="IWM23_001244"
     CDS             complement(28566..29072)
                     /locus_tag="IWM23_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9883899.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            29232..29672
                     /locus_tag="IWM23_001245"
     CDS             29232..29672
                     /locus_tag="IWM23_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9883900.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            29831..31171
                     /locus_tag="IWM23_001246"
     CDS             29831..31171
                     /locus_tag="IWM23_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9883901.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(31240..32397)
                     /locus_tag="IWM23_001247"
     CDS             complement(31240..32397)
                     /locus_tag="IWM23_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883902.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(32400..32756)
                     /locus_tag="IWM23_001248"
     CDS             complement(32400..32756)
                     /locus_tag="IWM23_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883903.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(33054..>33390)
                     /gene="sgrA"
                     /locus_tag="IWM23_001249"
     CDS             complement(33054..>33390)
                     /gene="sgrA"
                     /locus_tag="IWM23_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9883904.1"
                     /translation="TGSMSSESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSN
                     LKSVLKQPDFVSRGIDHQEKAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASY
                     FRSLFHRVK"
BASE COUNT         9709 a         6653 c         5376 g        11652 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt tttttcgata cgattatctg actgatctag attaatcact
     6601 accaaagaaa atggatgctc tagtttcgtc tcataaaggc gctccatccg agaaacttct
     6661 tccggatatt cggttcctac gaaaatatca tcaggaagct cgaattcttc gattgcttgc
     6721 tcaatctctt cgggctttgc actatcaagt acgagccaat tatatttttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgttg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa
    13981 agacggttaa aagtcaattt ttattaagct ttaacaatgt ttgttgcttg agggccacgt
    14041 tgaccatctt caacatcgta agacactgct tggccttctt ctaaagtttt gaagccgtca
    14101 ccttggatag ctgaaaaatg tgcaaataca tcatttccat cttcaccagt gataaagcca
    14161 aaacctttgt ctgcgttgaa ccattttact gtaccgttat tcataaataa attcctcctg
    14221 atacgtatca atgtacgttt tttcgttgca ataaatattt gataggcaaa aggagtttta
    14281 tctatgtaga tatatcgctc aaattacgtt tcaaaataga ttactcattt aatatatcat
    14341 gctttttcta ataacacaag gataatgcaa tgaaatataa aattcattgt cttctattgt
    14401 cttcgaatcg aagaaaaagt tgaataaccg cgattgattc actttttgtt ttcttgttta
    14461 tttgctttta tctgcaactt ttgactttaa aatttcttaa ctatgaaaac ctttcaattg
    14521 gtggtatact tataaatgga atttcgttat caacgcgttc gttgcctttc agcaagtatt
    14581 taaagaaagg agaatggcta tgacattcta taacaaagat atacaatatc gagtaacctt
    14641 ggatacagat ttgaatctat ttgtcgtttt tgacaaagaa aataccaatc atgtagcgac
    14701 aggtgttacg atcgaacaag cagtgcaaga attaaaaaaa tctgcataag gagtggaaac
    14761 cctcatatgg acgattctcg ttcttctctt taaacgcatc tactggtaga taacagtaga
    14821 tgcgttttgt gtttgttcta aattgttcac tacaaataag gtagatactt caaaataaaa
    14881 aattacttct ttttcatctg ttccctcagt cgtgcaggac aataaataaa gaggactaga
    14941 aaatagtcct ctttattaga gatacaaccc tttactttct aaaaatcgtt ttgcttgatg
    15001 caggtcataa aattcatagt ctgctttatt ggattcatat aaacttacat ctactttcaa
    15061 cttatgcttc ccattatgat cgggcaaccc tatcaaatga tctttgatga gaaaagacaa
    15121 aatgcccgct cgccttgctg cttcgatccc tgcaatcgag tcttcaaata caagtgcttc
    15181 acttggagga atattcattt ttgaacaagc tttcaaaaag atctctggtt ccggctttcc
    15241 tttttttaca tcatcccctg aaatgatttg atcaaacaag gcatatagat tttccatttt
    15301 taaatacatt tctactttta cccgtgtatt agacgaagcc acgattgtat ggattcctct
    15361 ttcttttgca aaagataaaa tttcttctgc tccttttttc gtatatactc tcttatcttc
    15421 tttcacgatt ttttctttat attgattgat tgcttctcgc catttgatat atggaacatc
    15481 ttctccgtac aaaatgccca tttgttctct tatcgccatt tctgtttggc cagttagtaa
    15541 ataatagact tccttacgca tttcataatc atacttttgt gctgatttta cataagcacg
    15601 ataagctaaa cgtccagttt ctaataaaag gccatccata tcaaagatga agagcttgat
    15661 tttattcata atgctctccc agtggttttt ccttacttat cgcctcatat agaccttgca
    15721 tgtaagtgag tcccattgca cgatcataca ggccatatcc aggcatagcc acttcacccc
    15781 acacggttct accatgatca gggcgtatag gaccgtcgaa cccttcttgc accaacgctt
    15841 tcataatatg atacatatca agggagcctt ctgtagaggg atgtgccgtt tctttgaact
    15901 ttctttctcc taaaaactcc acgtttctga agtggacaaa atggatgcgc gaacctacct
    15961 catggatgat ttcaactaaa tcgttttccg gatttgcccc taaagatcct gtgcacagcg
    16021 ttagtccatt atatggagaa tctactgcat ttagaatttt cttcaagtcg ccaagatttt
    16081 tcgtcactct aggaaaacca aaaagttccc atggcggatc atctggatgg atagccattt
    16141 ttacatctgc tttttcgcac actggaatca ctttttctaa aaaataagtc agattttcaa
    16201 ataattcttc ttcggtcatt ccttcataca gtttttccag ctgagcgaat tttttcaacc
    16261 gttcttcttc ccaacccggc aatttgaatc cctttgactg gctgtgtatc aattgataca
    16321 tttctcctgg ctccatctga tcgacgattt tctgatcgta taaaagagaa tagcttccat
    16381 cgttgttttc attaaacaaa tcagttttcg cccatccaaa aattggtttg aagctataac
    16441 aaatcaatcg aaccccacat cgagacaagt ttttgattgt tcgtatatag ttatcaatat
    16501 agtaatctcg ttcatctgtc cctgccttga ttgcatcgtg gatcgctaca ctttcaattc
    16561 caagcagttc cagtccttgg ctctcaacag atgctttcaa ctcctcaatt tctgaaatct
    16621 cccaaacatc tccaggcatt ttagtcagca aagttccaat tatcccattt attcccggta
    16681 tttggcgaac atattttaat gggatcgaat cccctttttc accataccat ctaaatcccc
    16741 acttcatctt ttcagccttc cttctaaaaa tcgtcctcat ctaatttttt ttcttccgct
    16801 gaagcatgcc aaacagttaa attatctttt ccttgtttga caatttgttc caccgcttct
    16861 tctatcggtg tgtgaagcat ctgatgattg attgcttcta acaacattgg cagattcacg
    16921 ccccctatga cataagtggc tgctttcttt tcttcagtca gttcattgac tgccaagagc
    16981 gcttgattgt acggacttgc gctaattaga tcaacaaata ctagcatggg ttctgctgtg
    17041 ttgatcatag ttaatttttc tttgaccttc tctccgaatg tttggatgtc ctccccagac
    17101 tctaagcaca ttgtttcaat atggctcgat gtcccgacaa ttacttccgc cgcatctttc
    17161 agtccttcgc tcaacttacc atgtgtaacg atcatgattt ccaacattag tcttctcact
    17221 ccttgttttg cactttttac ttatcgaaaa agagcaataa tctgattggc attttgccaa
    17281 ttaaatgttt tccctcgtaa atctgttttg gtcaaataga agaactgcgc ttgctttttt
    17341 cttgcactaa aaatataatt gacgtttttt tctttcaact tatcaacttg tttcagccac
    17401 tgttctttat ttgaaaaata atttcccgaa aactgtagcg gtgtatctgc cccaacagag
    17461 tctagataaa aaagaatagc ctcttttttc atacccgatc tcacgctgtc taagcccttt
    17521 tctccataac agccttcatc aacaaacgca tacgctacac tcggatcatc aatcgtacgg
    17581 atcatttcta gcatcgttaa tatggatgaa gtatttcgaa taaaagtttg tctgctaggc
    17641 catccgcgag ctgcttttcc caataagaaa aagtagctcc caaatgctag tacagtgata
    17701 cctgtttata cggatagcag atcgaatacg tgatgaggat ttttgttata tatcatcatt
    17761 ccccaccaac ctaaactgaa caacaaaatg aaggacaata atatagagta gattgttttt
    17821 ttcctttggt ctttccaatc aaacatgaaa taacttccta atgcatgaac gggtgtatcg
    17881 taataagtac agataacttt ctctgctttt tcgatatctc caacataaat attttttgat
    17941 cggtaggcta gcgtatcgta agcaatgact gtcacatctg ttctcatact gtaaatatct
    18001 gatactaatg ctgaaagaaa acgctgttta ctcttttttg tacggcgctt acggtaaata
    18061 tagcgataac gaaattccca atcacgaaac agctcgttct tcttcatttt ttcgccctct
    18121 ttatcttttt tctaagcatt tgtttatgac aaatcaaact tgttcaatac atcttttaat
    18181 cctgttttag gtgcagatgg agtggcttga aaataaatat tctttacgcc atattgttca
    18241 ttcatttctt tcaatttttc agcatctgat ttatttaaat aaacagattt cgtcactaaa
    18301 agatctgtat caggattttt ttgagcaatc cctccaatat tcaattcttc catggggatt
    18361 ccgtgtttta ctaagtcgta aatcgtttgg actgtttttg cgatcaaaat acaattgtat
    18421 tctttaaatt gatattcatc ccaatagaat ttagctttct ctggtgtaat gacaattgtt
    18481 ttttgtcctg tacggcttcc tgcatttttg taaagatctc tcataaattt atcttttgct
    18541 acaacttcgt ctacaacaaa aatggaattc actccacttt tttgagataa cgttaccatt
    18601 acttgcccat ggatcaaacg ctcatccaca cgcgctaaat tgactacacc catcttattc
    18661 acgttccctt ctttttataa aataccgatt cctgctagca caattaatat tccagtcagc
    18721 cataataatg cttgtgtgac tttcaatcct tttttcgtat agaacaagta cacacccatt
    18781 acgacagcta aaggtaatat ccctggcacg atttgatcaa gaatttcctg gataacaaac
    18841 tcacgtccgg atatcgcaaa tttcaatgaa gattctactt ttacatagtt tcccgcaagc
    18901 acccccatca taaacaaccc taaaactgat agcatttcaa tcgctgtctg aacaccagtg
    18961 ttttgcttta ggcctgtagc attacggccc atagtgaaag cttgtcttgc gaaaaatacc
    19021 cctagtaaaa cctgatacaa cccaaagcag ataaaaggaa aaagtgcgcc taaaggactt
    19081 ccctgttgtg cccaaggtac cgcgattgcg ataaaaatat attggacggt tcctgaatcg
    19141 attgcatctc cgattcctgc tagtgccccc atcaatcccg ctttcgtgtt atacatcaag
    19201 tcatcctgca tcaagatctc tttttcttca tactcttctt ctgctctttg ctgttccata
    19261 gaagacatta ggccagtaat cacaccgcca ccccagctta attgagtgtt gaagaacagt
    19321 aactgccgtt tatatgctgc ttttaaagag tcttcatctt tataaagttt tttcaagatt
    19381 ggcatcattc cgtatagtag tgatggcgcc aagtaacgtt caaaagagtg aggaatttcg
    19441 tttgcaaaat gccatctcag ataggctttt gttacatctt ttttcgtgat ttcttcagga
    19501 gcacctgttt gattatccat tatttcagcc atttcccatt cctcctaaat taaaatccgt
    19561 catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg gcaggctgta
    19621 cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca aaaatggcat
    19681 aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa gcaaggaaga
    19741 aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata ccgattgccg
    19801 ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct aaagctttga
    19861 ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc ggaacgaata
    19921 ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct tttgtattca
    19981 aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa aaacgaaact
    20041 gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct gcttctggcg
    20101 acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat ggttcagctg
    20161 gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg gctgtaacaa
    20221 tcatcgcttg tgcaacttcc cccatgatca acccgacgac taatccagtc atcaaaggag
    20281 aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc gcggctaccc
    20341 ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct cctccttcag
    20401 atctttctga tataaccttc cagttccatc attgactgta tcagtgattt ctgtgttacc
    20461 tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat tttatctatt
    20521 atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag ttctaattcc
    20581 cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc cagttgatag
    20641 aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg aatatattta
    20701 ctgatagcgt catgcatagc atgtgctgcc acatttcttg cgtatttatc tcctaatcct
    20761 ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc cgatgtcagt
    20821 tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc ttctttgcag
    20881 atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa atcttgttct
    20941 aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc aatgaaatat
    21001 ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc ttgtctgtaa
    21061 aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc aagaggtgtc
    21121 catggagcac aattactagc taaagtaggg atgactccga aatcgattct tgcaagattg
    21181 gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac gaaatcgatt
    21241 tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat tctccagaat
    21301 aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta ggtagcgcct
    21361 tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat gatttcaaga
    21421 gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg cctgttttca
    21481 cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct ttgtctggac
    21541 agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac atttttcacc
    21601 cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca ctacttgttc
    21661 aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat ctgtggcata
    21721 tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa ctagagcgcc
    21781 tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt tatcagaaat
    21841 catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg attgttccag
    21901 taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat ctgatttgta
    21961 aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga ttactccgct
    22021 tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc taccgacgaa
    22081 ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg tcgtgcgtcg
    22141 aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt caactaaagc
    22201 aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa tattgttgta
    22261 accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa aatctcgtcg
    22321 aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact ccgctagttc
    22381 ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg actttaatct
    22441 ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg ctatatgata
    22501 atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca tttcttagtg
    22561 ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc atgaacaaac
    22621 tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc gtccattttg
    22681 cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt tcgtttaact
    22741 gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc caaacacgtg
    22801 cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat ccttcaccga
    22861 ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag tgtccgcttc
    22921 cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat aattgttcca
    22981 cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt cgtgctcctt
    23041 cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga aactcttctg
    23101 ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg tctcctggtc
    23161 cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca ggaagcatta
    23221 accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct tgataagcag
    23281 gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt ccttgtactt
    23341 tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc aaaccaatta
    23401 caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa aaaatttttt
    23461 tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc cttgtatctt
    23521 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
    23581 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
    23641 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
    23701 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
    23761 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
    23821 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
    23881 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
    23941 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
    24001 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
    24061 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
    24121 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
    24181 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
    24241 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
    24301 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
    24361 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
    24421 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
    24481 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt
    24541 agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt
    24601 agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt ttttttatta
    24661 tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag tatttccttg
    24721 ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat
    24781 atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg aaattctttt
    24841 gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg tctagacgta
    24901 tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc
    24961 tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta
    25021 tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta
    25081 tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt
    25141 cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac
    25201 tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat
    25261 caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg
    25321 atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt
    25381 tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc
    25441 taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa aaacattact
    25501 aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc
    25561 aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat
    25621 acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat
    25681 gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat
    25741 tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag
    25801 attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac
    25861 ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca
    25921 ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc
    25981 gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg
    26041 ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt
    26101 acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta
    26161 tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc
    26221 actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct
    26281 ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt
    26341 tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt
    26401 tgattaatca aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact
    26461 attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat
    26521 cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt
    26581 tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat
    26641 cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc
    26701 attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat
    26761 aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc
    26821 gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg
    26881 gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact ttacgtttat
    26941 ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc
    27001 aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc
    27061 tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg
    27121 gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtatc
    27181 caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg
    27241 ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac
    27301 tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat
    27361 aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc
    27421 catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat
    27481 ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt
    27541 accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta
    27601 ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt
    27661 tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt
    27721 gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg
    27781 cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc
    27841 gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat ctacaccata
    27901 atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt
    27961 tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt
    28021 gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt
    28081 agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt
    28141 atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc
    28201 agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc
    28261 tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca
    28321 ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt
    28381 tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca
    28441 ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat
    28501 tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa
    28561 acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg
    28621 attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc
    28681 ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct
    28741 tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta
    28801 gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat
    28861 aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga
    28921 acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct
    28981 gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg
    29041 atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat
    29101 ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca
    29161 cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat acttagctat
    29221 tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg
    29281 caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac
    29341 cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa
    29401 tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt
    29461 tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct
    29521 acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt
    29581 ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt
    29641 ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctgg
    29701 atctataata tctagtgttg gtgacgcctt ctgttgccaa tactttttct tttttctaat
    29761 aaacgcaaac agacaccttg aaatccttta gaataaagtc gacgaaaacc attcaaagga
    29821 gtgtttcaag atgtcctatt cagagtttac caaagagtta ctagatattc tagacctaaa
    29881 tcttaccttt catgaagatg cctttcgcaa agagcgcatt aatgacgaaa catgttttgt
    29941 ttttgatggc acattgacct atcaaccaga ggaatgcttc cattgccact atcaaaataa
    30001 gcaaacgatc attaaatggg gctggaaaaa agtatccatc ttattgaatg atgtcagtaa
    30061 ctacaagacc attgtgcgga ttaacaaaca gcgcttcaaa tgtaaacact gtggaaagac
    30121 ttttttagca gaagattcag ttagcgatcg tcgctgctcc attgcccgca gagtcaaaca
    30181 agccattctt gagctattaa gtgagcctat ttctatgtct ttgattgcac gtatgaagca
    30241 catttctccg accactgtca ttcgaatact tcgcagcctt cggcctaaaa ccgtttcttt
    30301 gaaccagccg ttaccagaag tggtgtgttt cgatgaattc aaatcggtga aaaacgtctc
    30361 gggagccatg agctttgtca tgatggacgg caagacccat caactgattg atattgtgga
    30421 aaatcggcaa ctcaatcctt tacgcgacta tttcttgcgc taccctagaa aagtccgtga
    30481 acaagtacgt ctagtggttt cagatttcta tacaccctat cgcacattgg tgaaagagtg
    30541 ttttccgaat gcccgaatcg tcgccgatcg ctttcatatt agccaacaca tcggtcgggc
    30601 ctttaccaac caccgaattc aagtaatgaa gacctttaaa aaaggggatc gtcggtcgaa
    30661 gcacttaaag aaatactgga gactacttca aaagaatgcg tgggaattga atggtcagca
    30721 tcgctactgg cgtccgtcat ttcgagatca tctgacagaa gcggagatag tcgatcggct
    30781 attatattat gatgactcct taaagcgagg ctacgaagtc taccaggttt ttctttccgc
    30841 tatcagaaga caagatgttc ctgaattcgt cgcgctccta aaagaagact acaaggagtt
    30901 gccagagcac tatcaaccag tgttcacgac cttcaagaaa tatcgaacag aaatcaaacg
    30961 agcgttgcga gttccctatt ccaacggccc tatagaatgt ttgaacaacc atatcaaagt
    31021 actaaaacga attgcctatg gctttagaaa ctttcaaaac taccgagaac gaatcttttt
    31081 atatcgagga aaatatttca agaaaactaa gaacactacc caactgacta aagccagaac
    31141 aacaacacgg ctagacaaga tagctgtcta gccgtgtgag tatctcgatt attctattga
    31201 atttcttcac caacactcat tgacgaagag ccgcttttct taattaacga aatccaattt
    31261 ttccaatatg gatctatttt tctcattcct gatttttatg atttcagctt tcagacgcgc
    31321 ccaatcattt tttgtaggtt gtccattcca gtaaaatatc tgtgtattcg tttgatcata
    31381 tgcctttttg tcgataggat aatcgctaat gacaatatca ggtaactgat cagcggatgc
    31441 actatatttt attgggcgtt cagtcataga taatatcctt ttctttgccc ataattcctg
    31501 tgttttacca tactttgatt gaatatagaa tatgacaggt tgttcgtgtt ttataaaaat
    31561 atccctgact aacatacagt attgaaaaat caaccgtctg ttcttttcat aataagtatt
    31621 aagctctgga attctgcgag ctaccccact aaaaaactgt tgcatgattg gatacatcac
    31681 tggaaatata tgctgaaaat ctctgtcttc tgctgatttt ccaaaagcat ccaccttctt
    31741 ttttgtccca aaaacccttg aatacaaatg gatcattgcc aaattgacat atagataatt
    31801 tttttctgaa gttgtaaaac aaatcttgaa gtttttttca aaattttcta caatagacga
    31861 ttcaatccag ccccaacttt cttgtacttt atctggagta tccaacaaat cttcttccaa
    31921 ataggtgttc attacaccaa acataaaata aaggtaagct tgctctgttg gagtgatgtg
    31981 attcatcgac cagaagtttg cttcaaaaaa aggctctagt atgtaatgcg taaactcttc
    32041 ttttgttatc aatggatgca caaatttgtt catctcttct gggacatacg taatcgagtg
    32101 gccttgtttc attcgttgca acgaaattct tagtaggctg atccatttct ttctatctat
    32161 actacgcgta tatggcgcat gtttcgcaaa tttttctaat agttgacgtt tattttttat
    32221 acttgataat atatcagaat gattttcgtc gaatgactgc caatacatca agtgaaaaaa
    32281 gtatcggata cggtattctt ctccttctat cggattacct tttttcaagc tgcaagataa
    32341 atgatagttt ttcaaaatag tattaagttc tcttaatttt ctttgaatcg tacgcaaact
    32401 tatataattt tttgactaag attttctaag gattgataag tacctttgta aagtgaatcc
    32461 agtaaaatat aaccaatcgt tccctctaaa agtatagaat aaactttact cagtaaaaac
    32521 tgagaagaaa actcgacttt tatcatcttg ttttctgaaa gaaaagataa tttatattct
    32581 ggataccttt cgcaaaacga ttctgcttgt tgaatcaccg aattcatctt agccagggtg
    32641 tagtttagat ctaaagcgat atccttcacc acatataatt tctcgtctct ttcataacaa
    32701 tagttcaaaa attgatatac ttgcctttca ttgatatcaa taaattcttc aaacaaagta
    32761 catactcctt tttatctatt atttagactt attccgacag ctacatgaat aacgtacaaa
    32821 tagattcgtt actgattttt cagagatact accatgcgca gtgtgcattg tcaattattc
    32881 ttgctcttat tttccatttg tgttcctgtt atttcctgtt tctcaaatat gttagtaaac
    32941 aaaaatagat ctggcaaagc tacaacttcg ccaggcaata cagcactctc gaatgaaata
    33001 ttcactcaaa aatctatcgt tttattcaat tttagatctg ttgcttttat tctttatttc
    33061 actcgatgga agagagaacg aaaatagctt gctcctacaa tcaaaatgag taacacaccc
    33121 atccatgtaa ttagcttgtt caaggttcta ctaccagttt ctggtaaatc gctttttcta
    33181 ttatctgttt ctttcgcttt ttcttgatga tcaattccac gagaaacaaa atcaggctgt
    33241 tttagcacac tttttaggtt gctccctgca tttctctttt gttccttagt tggtatgata
    33301 ggtaccaaat caagttcatt atttactttt cctgtgctgc tttcgctgct cgtgcttcct
    33361 gtgctactct cactactcat gcttcctgtg
//
LOCUS       DACDXE010000026        32176 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.026, whole genome shotgun sequence.
ACCESSION   DACDXE010000026 DACDXE010000000
VERSION     DACDXE010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.026"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(24..203)
                     /locus_tag="IWM23_001250"
                     /pseudo
     CDS             complement(24..203)
                     /locus_tag="IWM23_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(407..766)
                     /locus_tag="IWM23_001251"
     CDS             complement(407..766)
                     /locus_tag="IWM23_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAP9883905.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="IWM23_001252"
     CDS             complement(796..1137)
                     /locus_tag="IWM23_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883906.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="IWM23_001253"
     CDS             complement(1134..1808)
                     /locus_tag="IWM23_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9883907.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWM23_001254"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="IWM23_001254"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAP9883908.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="IWM23_001255"
     CDS             complement(4322..5512)
                     /locus_tag="IWM23_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9883909.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="IWM23_001256"
     CDS             complement(5533..6060)
                     /locus_tag="IWM23_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9883910.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="IWM23_001257"
     CDS             complement(6107..8896)
                     /locus_tag="IWM23_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAP9883911.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="IWM23_001258"
     CDS             complement(9043..9243)
                     /locus_tag="IWM23_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9883912.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="IWM23_001259"
     CDS             complement(9696..10016)
                     /locus_tag="IWM23_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883913.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="IWM23_001260"
     CDS             complement(10519..11637)
                     /locus_tag="IWM23_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883914.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="IWM23_001261"
     CDS             12108..13415
                     /locus_tag="IWM23_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAP9883915.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="IWM23_001262"
     CDS             13536..14735
                     /locus_tag="IWM23_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9883916.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="IWM23_001263"
     CDS             complement(14762..16030)
                     /locus_tag="IWM23_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAP9883917.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="IWM23_001264"
     CDS             complement(16200..16841)
                     /locus_tag="IWM23_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HAP9883918.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="IWM23_001265"
     CDS             complement(17250..17753)
                     /locus_tag="IWM23_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883919.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="IWM23_001266"
     CDS             complement(17876..18640)
                     /locus_tag="IWM23_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAP9883920.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="IWM23_001267"
     CDS             18748..19023
                     /locus_tag="IWM23_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAP9883921.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="IWM23_001268"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="IWM23_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAP9883922.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="IWM23_001269"
     CDS             complement(20579..20980)
                     /locus_tag="IWM23_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9883923.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="IWM23_001270"
     CDS             complement(20994..21803)
                     /locus_tag="IWM23_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9883924.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="IWM23_001271"
     CDS             complement(21790..22680)
                     /locus_tag="IWM23_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9883925.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="IWM23_001272"
     CDS             complement(22693..23172)
                     /locus_tag="IWM23_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9883926.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(23348..24289)
                     /locus_tag="IWM23_001273"
     CDS             complement(23348..24289)
                     /locus_tag="IWM23_001273"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HAP9883927.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWM23_001274"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="IWM23_001274"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9883928.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="IWM23_001275"
     CDS             complement(25274..26470)
                     /locus_tag="IWM23_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9883929.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="IWM23_001276"
     CDS             complement(26488..27216)
                     /locus_tag="IWM23_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9883930.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWM23_001277"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="IWM23_001277"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9883931.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="IWM23_001278"
     CDS             complement(28628..29086)
                     /locus_tag="IWM23_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9883932.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="IWM23_001279"
     CDS             complement(29083..30546)
                     /locus_tag="IWM23_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9883933.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWM23_001280"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="IWM23_001280"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010779385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9883934.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32176
                     /locus_tag="IWM23_001281"
     CDS             31602..>32176
                     /locus_tag="IWM23_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9883935.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFF"
BASE COUNT         9384 a         6920 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgtcatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcaacaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaa
//
LOCUS       DACDXE010000027        31842 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.027, whole genome shotgun sequence.
ACCESSION   DACDXE010000027 DACDXE010000000
VERSION     DACDXE010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31842
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.027"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..2304)
                     /locus_tag="IWM23_001282"
     CDS             complement(151..2304)
                     /locus_tag="IWM23_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAP9883936.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="IWM23_001283"
     CDS             complement(2598..3446)
                     /locus_tag="IWM23_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9883937.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWM23_001284"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="IWM23_001284"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAP9883938.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="IWM23_001285"
     CDS             complement(5947..6126)
                     /locus_tag="IWM23_001285"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAP9883939.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWM23_001286"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="IWM23_001286"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9883940.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="IWM23_001287"
     CDS             8274..10553
                     /locus_tag="IWM23_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9883941.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="IWM23_001288"
     CDS             complement(10646..11293)
                     /locus_tag="IWM23_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883942.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="IWM23_001289"
     CDS             complement(11472..12191)
                     /locus_tag="IWM23_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9883943.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="IWM23_001290"
     CDS             complement(12322..13473)
                     /locus_tag="IWM23_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAP9883944.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="IWM23_001291"
     CDS             complement(13690..14430)
                     /locus_tag="IWM23_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9883945.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWM23_001292"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="IWM23_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAP9883946.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /locus_tag="IWM23_001293"
     CDS             complement(14814..15407)
                     /locus_tag="IWM23_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAP9883947.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16038)
                     /locus_tag="IWM23_001294"
     CDS             complement(15391..16038)
                     /locus_tag="IWM23_001294"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAP9883948.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWM23_001295"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="IWM23_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAP9883949.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWM23_001296"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="IWM23_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAP9883950.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="IWM23_001297"
     CDS             complement(17484..18011)
                     /locus_tag="IWM23_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAP9883951.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="IWM23_001298"
     CDS             complement(18191..18976)
                     /locus_tag="IWM23_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAP9883952.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="IWM23_001299"
     CDS             complement(18969..19838)
                     /locus_tag="IWM23_001299"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAP9883953.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="IWM23_001300"
     CDS             complement(19839..21218)
                     /locus_tag="IWM23_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAP9883954.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWM23_001301"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="IWM23_001301"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9883955.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWM23_001302"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="IWM23_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAP9883956.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWM23_001303"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="IWM23_001303"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAP9883957.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWM23_001304"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="IWM23_001304"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAP9883958.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWM23_001305"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="IWM23_001305"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAP9883959.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="IWM23_001306"
     CDS             complement(25176..25400)
                     /locus_tag="IWM23_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAP9883960.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="IWM23_001307"
     CDS             complement(25453..26415)
                     /locus_tag="IWM23_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAP9883961.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="IWM23_001308"
     CDS             complement(26425..26865)
                     /locus_tag="IWM23_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9883962.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="IWM23_001309"
     CDS             27171..28136
                     /locus_tag="IWM23_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAP9883963.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="IWM23_001310"
     CDS             complement(28193..28423)
                     /locus_tag="IWM23_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAP9883964.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29396)
                     /locus_tag="IWM23_001311"
     CDS             complement(28509..29396)
                     /locus_tag="IWM23_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAP9883965.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29485..30708)
                     /locus_tag="IWM23_001312"
     CDS             complement(29485..30708)
                     /locus_tag="IWM23_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9883966.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30725..31804)
                     /locus_tag="IWM23_001313"
     CDS             complement(30725..31804)
                     /locus_tag="IWM23_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9883967.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8794 a         6970 c         5488 g        10590 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgtatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 cttggtgtac ttgtagtcaa atcaactaaa atatgtcctg agatatctgc agaaaaaatc
    29161 cccgactgtc catagtagat ttcttctaca tctttggggt aacctaccat tgtaaagaga
    29221 atattacttt gttctgctac cgcttttggt gtatctgccc agatagctcc ttctgttact
    29281 aaaggatcag cttttgcttt tgtccgatta tagacagcta cttcgtgtcc tgcttctagt
    29341 agatggcgtg cgacagcact tcccatgacg cctgttccga taaaaccaag tttcattttt
    29401 tcgacctcct aaaaatcaaa aatccttgct tctatgctag tatacgctag aaacaaggat
    29461 tttttcataa aaaatgtttg aattttatct tttaaatgaa tttaatttta ttttgattcg
    29521 ttcaatcgag tcttcttctt cttttcttcg catctcttct tcatgataaa caaccatgtc
    29581 ttgataaaat tcgatcatag cttcgccgaa atgtgtagat gaaatatcat aaagtttagc
    29641 ttctaaaact tttggatctt taggaacttc actggaaata taatcaataa gtgctggtgc
    29701 aaagtcttcg tcggtttcaa aagttttccc taatgaagga tgatcgaaca atcggttcaa
    29761 ataagcattt ccttccacga cacaaggtgt tccggctgcc atcgcttctg tataagtaag
    29821 cccttgtgtt tctgaagtag aagcactgac aaaataatct gccgctttat aataaagtgc
    29881 tacatcattg ttgtccactt ctcctgtgaa ctgcacaaat tcttctaact gcagttctga
    29941 gatcaattcc tctaactctt ctacataagg tccttttcct acgataacta atctggcgtt
    30001 aggataagcg gagatgacct gaggaaaccc attaatcaag gcttggatat ttttctcata
    30061 agaaatcctg cttaaagaaa gaatcatcaa atggttttcc tgaagaccta gtgactccct
    30121 taatttgctg atatcctgct gagtgatatc agaacgtttg aatttttcaa tgtcgatacc
    30181 agtaggaatc acgcgaagcg gtgctattac accatagctg cgtaatgtat caataacacg
    30241 ttcacttgga caaactacac ctgtcgtatg atttgcaaat aatctagaca agtattttac
    30301 atgggattgc ctgatcactt ttcctttacc gatataatga aggtagtctt catacatcgt
    30361 atggtaagta tggatcagag gtattttcaa cttcttagct accatcttcc caagtatccc
    30421 tgctccgaat tctgtatggg tatgaataag atctagttct agttctttgg caaccaaata
    30481 agcataccac atgcctctaa caacaactct tcgatccttg aaagaaataa atggtacact
    30541 tggcattcga atcacatctt tttccaactt atccgcatta ggatcggtcg ttgtaaaaat
    30601 atagacttca tgcccatgtc tttctaattc ttgttttaat gttttgatcg atgtagcaac
    30661 tccgctgact tgcggaaagt aggtatcggt aaaaaagccg attttcatgt ttttcgcctc
    30721 caaactagtc cttgacctga tttacttttt ttaatttgct agagactttt tcattcagta
    30781 cactttcgta tacattttta agttcttcac cgattttatt gatacttcgc tcttttgctg
    30841 tttgataacc ggcttgtgtc gtactcggta gttcttgatt ggtaatctgt tggatcagtc
    30901 ggataaattc ttcgtttgtc ttccccatat agcagttctt gctatcttct aaccagccat
    30961 tatacactgg aatatctcga acaagtacgt tttgtttgct tgctaaagct tctaaaacaa
    31021 cgatcccttc tgtctcttca taagaaggga aaaagaatag atctgcacct gaatacgcac
    31081 cttctatgat ctctccacga atgtaaccag gaaacaggac attttcagga tgatcttctt
    31141 ttacaatttt ccggatgttc cgaggaatcg agtacatggg aacatgacca aaccaaataa
    31201 atgtgtattc gggcatcttc ttagcaattt ctacgaaatc aatgattcct ttacgttcga
    31261 aaaacaaacc gacacaaatg attacttttt gatccggaga cagtttgaaa tattctctga
    31321 acttttgttc tttcattgga tcagggtgat atttctctaa gtcgatccca ttggaaatag
    31381 cttgaatcgg cacacggata ccatagcttt ccaacaactg ttttgaataa ggtgttggcg
    31441 tgatcagata gtccgctttt tggtacaatc ctaccagata tttttttacg ataggagaaa
    31501 gctggttcga tccgataaag gaatttcgga aatcttcctc tgtagaatgc gcatgataga
    31561 caactttttt tccttctctt ctggctttat tgaccatatt atgactattg attccgtatg
    31621 tattgatgtg caggatgtca taatcttctt tttcatctag tgtataagag ataccgactt
    31681 ccgtcagagc acgtttttga tgatcaagcg cacgcccgat tccagatttt gcaagcatct
    31741 tttcactttc aaaatataat aataccttca ccttacgtcc cccttcactt atatagtata
    31801 acatagccta caaactgtca ctcaaactta agccctattt tt
//
LOCUS       DACDXE010000028        31791 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.028, whole genome shotgun sequence.
ACCESSION   DACDXE010000028 DACDXE010000000
VERSION     DACDXE010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.028"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            501..1412
                     /locus_tag="IWM23_001314"
     CDS             501..1412
                     /locus_tag="IWM23_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883968.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            1899..2165
                     /locus_tag="IWM23_001315"
     CDS             1899..2165
                     /locus_tag="IWM23_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883969.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2237..2578
                     /locus_tag="IWM23_001316"
     CDS             2237..2578
                     /locus_tag="IWM23_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883970.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2633..3052
                     /gene="ssb"
                     /locus_tag="IWM23_001317"
     CDS             2633..3052
                     /gene="ssb"
                     /locus_tag="IWM23_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9883971.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3129..3425
                     /locus_tag="IWM23_001318"
     CDS             3129..3425
                     /locus_tag="IWM23_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883972.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3440..3871
                     /locus_tag="IWM23_001319"
     CDS             3440..3871
                     /locus_tag="IWM23_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883973.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4053..4358
                     /locus_tag="IWM23_001320"
     CDS             4053..4358
                     /locus_tag="IWM23_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883974.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            4762..5250
                     /locus_tag="IWM23_001321"
     CDS             4762..5250
                     /locus_tag="IWM23_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9883975.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6584..6886
                     /locus_tag="IWM23_001322"
     CDS             6584..6886
                     /locus_tag="IWM23_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9883976.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            6879..7907
                     /locus_tag="IWM23_001323"
     CDS             6879..7907
                     /locus_tag="IWM23_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAP9883977.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            7963..8181
                     /locus_tag="IWM23_001324"
     CDS             7963..8181
                     /locus_tag="IWM23_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883978.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            8252..9067
                     /locus_tag="IWM23_001325"
     CDS             8252..9067
                     /locus_tag="IWM23_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883979.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNIIDIGDYSME
                     EKKIYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNSKELEVVLRYNL
                     FSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTTILTTVLENINE
                     VSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVLRYAYIY"
     gene            9131..9466
                     /locus_tag="IWM23_001326"
     CDS             9131..9466
                     /locus_tag="IWM23_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAP9883980.1"
                     /translation="MILLILSLVLLVVSNLMKNNPPTKINSFYGFRTKKSMKNEKSWH
                     LAQKLLGDFSKKKVIPVFLMGIVFFSIEIYSLAILKNENFFVSVLMGESLILILILCL
                     IVHEVNKQL"
     gene            9890..10153
                     /locus_tag="IWM23_001327"
     CDS             9890..10153
                     /locus_tag="IWM23_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883981.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            10469..10899
                     /locus_tag="IWM23_001328"
                     /pseudo
     CDS             10469..10899
                     /locus_tag="IWM23_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347687.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
     gene            11038..11460
                     /locus_tag="IWM23_001329"
     CDS             11038..11460
                     /locus_tag="IWM23_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883982.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            11605..11850
                     /locus_tag="IWM23_001330"
                     /pseudo
     CDS             11605..11850
                     /locus_tag="IWM23_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11959..12915
                     /locus_tag="IWM23_001331"
     CDS             11959..12915
                     /locus_tag="IWM23_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883983.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(13751..14422)
                     /locus_tag="IWM23_001332"
     CDS             complement(13751..14422)
                     /locus_tag="IWM23_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9883984.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            14739..16370
                     /locus_tag="IWM23_001333"
     CDS             14739..16370
                     /locus_tag="IWM23_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAP9883985.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            16373..17452
                     /gene="uxuA"
                     /locus_tag="IWM23_001334"
     CDS             16373..17452
                     /gene="uxuA"
                     /locus_tag="IWM23_001334"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAP9883986.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            17526..18953
                     /gene="uxaC"
                     /locus_tag="IWM23_001335"
     CDS             17526..18953
                     /gene="uxaC"
                     /locus_tag="IWM23_001335"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9883987.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            19037..19684
                     /locus_tag="IWM23_001336"
     CDS             19037..19684
                     /locus_tag="IWM23_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9883988.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
     gene            19813..21189
                     /locus_tag="IWM23_001337"
     CDS             19813..21189
                     /locus_tag="IWM23_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9883989.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
     gene            21200..21994
                     /locus_tag="IWM23_001338"
     CDS             21200..21994
                     /locus_tag="IWM23_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9883990.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKCLSGEYIDIEQKYIDTFWKNLTNK"
     gene            22007..23773
                     /locus_tag="IWM23_001339"
     CDS             22007..23773
                     /locus_tag="IWM23_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HAP9883991.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            23801..25609
                     /gene="uidA"
                     /locus_tag="IWM23_001340"
     CDS             23801..25609
                     /gene="uidA"
                     /locus_tag="IWM23_001340"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003479936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HAP9883992.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            25781..26182
                     /locus_tag="IWM23_001341"
     CDS             25781..26182
                     /locus_tag="IWM23_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9883993.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            26176..26421
                     /locus_tag="IWM23_001342"
     CDS             26176..26421
                     /locus_tag="IWM23_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002412373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883994.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(26745..26966)
                     /locus_tag="IWM23_001343"
     CDS             complement(26745..26966)
                     /locus_tag="IWM23_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9883995.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(27128..27200)
                     /locus_tag="IWM23_001344"
     tRNA            complement(27128..27200)
                     /locus_tag="IWM23_001344"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27165..27167),aa:Lys,seq:ctt)
     gene            27276..27506
                     /locus_tag="IWM23_001345"
     CDS             27276..27506
                     /locus_tag="IWM23_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9883996.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            27550..27873
                     /locus_tag="IWM23_001346"
                     /pseudo
     CDS             27550..27873
                     /locus_tag="IWM23_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            28216..28530
                     /locus_tag="IWM23_001347"
     CDS             28216..28530
                     /locus_tag="IWM23_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9883997.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            28546..28932
                     /locus_tag="IWM23_001348"
     CDS             28546..28932
                     /locus_tag="IWM23_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9883998.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            28961..30346
                     /locus_tag="IWM23_001349"
     CDS             28961..30346
                     /locus_tag="IWM23_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAP9883999.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            30349..30501
                     /locus_tag="IWM23_001350"
     CDS             30349..30501
                     /locus_tag="IWM23_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9884000.1"
                     /translation="MLAGVAIATPALNPPYLTGGYKSTGNSQKNIRKSFGYKGFTKFQ
                     RMSNGL"
     gene            30524..31729
                     /locus_tag="IWM23_001351"
     CDS             30524..31729
                     /locus_tag="IWM23_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9884001.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT        11356 a         4439 c         6231 g         9765 t
ORIGIN      
        1 aagtggcggt ttttccacgt tgagagaaaa tggtcaaaaa cctaagcggg aagtctccga
       61 ataacatacc aagcgccgac agccaacaga taaagaatac actactaaaa atagtagtag
      121 cataaaattt aaaagggaaa aaatcaaccc ctaccccagt cttggggtct caaaactaac
      181 tcccccctaa ccccctcttt cagcctcttt tgaaagaggg ggagaatcaa ccccacggcc
      241 aacgccccag tgttcttggg gctgtccaga acagaaactt gcgagagagt gggggtaaaa
      301 caagtgtaga ggaaacttga agacttgttc gaacagaaac ttgcgagaga gttggagtaa
      361 aacaagtgta gaggaaactt gaagacttgt tcgaacacaa acttacaaga gaggggagta
      421 aagcagttgc agaggaatca ggaagatttg ttcgaacaga aacttgcaag aaagcattat
      481 taaattgaag gtgatgaaat atgaagtatt tattgcatgg ctattttatt gattattcta
      541 ctcgcaatag tagtacttta gcgattctat gtttgcttca tgaattgaga gtggttacta
      601 gaaaacagct atacaaacta ttgaattttg acagatcaat tagtaaacaa ggagtaacga
      661 atgctttagt gagattatat gaaaaacagt taatcgatcg aaaaaaatat ggaacagaaa
      721 tgtattatta tttgacgaaa gagggacatc aaagcatagg tgggtattat acgttgccga
      781 aagttcctga atataatttg aatcaccatt tacaaattaa caattatttg attaaaggaa
      841 tggcattggc aaaagaccac ccacatttaa aacggattat ttcagaaaga cgtaccgtct
      901 atgaaacgaa ggatttaaat cctaagacaa aaggaagaaa attttttgtg ccagatttta
      961 tttttcgttt tcgagacaaa gaaaatcatg agatcaattg gtcctttgaa attgagctaa
     1021 cgttgaaaac acgtcgtcgc taccgtgaag cgatttttcc gaaatatatt cgtgagttgc
     1081 gaacacgtcc tgaatcacat ttaatttatg tgacacctag tggcgcaatt caagaagaac
     1141 ttcatgcgtt taagcgtatt tttttcaagc gcgaaactga aaaagatgcg agtcaacaag
     1201 aattgtttga gcggttgcat attttttcag ctttagagtt tgaagaggga ttaaaagcat
     1261 tattagagaa tgatccagca gtcaattggc ggacagtaga acgagaaaaa atttatccga
     1321 atggcgctgt tcaaagtcgt ttgaatttat ttgaagaatc aactgaaaat gaggaaaaac
     1381 caaaagtgaa gtttacgcct gaagtattgt aaaagagggt gttcgcttcg ctcttttttt
     1441 gtgccagttt gctggcaccg ttcagacgca agcgtctgaa ccggctcgtt tttaggaagg
     1501 actaacttgt ttgatttatc cgttgtcaaa gaagtggacg ttcgagagtc cctaatcgta
     1561 tagcagtcat tcggcgagta taacctaaaa cataacctgc caattgccaa gggtaaaatg
     1621 cacgctgcgc gcccttggca atcgtcagaa catgttttcg agccggttat gccgaacgac
     1681 cgctatacga aaagggcctc taaacagaag tccactttga tccttgacaa ctgaaaccca
     1741 aaatcaaaca agcaggcctt cgtctaaaaa cgagccgaat gaagaccttg ggggttgcac
     1801 ccccaaaccc ccgtgccccc gacacactac cgaaaaggtg ggggtgggtg gcgaaaaaat
     1861 caaataaaac attctattca agaaaggaag aaacaaccat gacaactaaa aacgagcaac
     1921 tgaaagaaca ggcaagaaaa gagttgcaag aaagaggttt gattattgag gggagctttg
     1981 aaggagattt tgaaacctat attggttgtt atgcaagacc gataaacaaa cctacagcct
     2041 tagacccaat gaacgaacaa gaagtaaaag aacaagaaaa acatgcagtg aatggttttc
     2101 cgcaagattt cactgaatgg tatgagtggg aaattaacaa cgggaagctt gaaaacttct
     2161 tataaaagtc agatgacgtg caaagagaat gattctcttt gtacggtaaa aatataaaga
     2221 aagaaggaaa aagaaaatgg caaaaaacac accttatcaa gtaggagaca ttttttatag
     2281 tagttggggc tacgaacaaa cgaacgtgac tttttggcaa attgtgaagt taacagaaaa
     2341 aacagcatgg tttcagccta ttcaaaagaa aacggtcgag cattattcgt ccatgagtgg
     2401 aaaagttgtt ccaatgaaag acaagtttat agagtatcct ttagcaaaaa ctaaagacag
     2461 tattgtaaaa aaacgaattg atccagaaac ctatcctgat atgttatgtg gttatcgtag
     2521 ttgggacacg tttcatagat ataaaaatca agctgtttat gaaagtagtt attattaagg
     2581 ggatagtttc ccctttcaaa aataataaat agaagaatgg agagaaaaaa tcatgatgaa
     2641 ttcagtgaat ttagtaggac ggttaacaaa agaagtggat ttaaaataca caccaaaagg
     2701 caatgcgaca ggcacattta ttttagctgt taatcgaaat tatacgaatg caaacggcga
     2761 gcgtgaagca gattatatcc gctgtgtcat ttggcggaaa gctgcggaaa ctttagcaaa
     2821 atatactgga aaaggaagtt tgattggcat taatggacgt cttcaaacga ggagctatca
     2881 gaatcagcaa ggacagacca tgtatgttac ggaagttcta gtgacagatt tctatttgtt
     2941 agagagtaaa gaaatcaatg agcaacggtc aaaatcaatg gcgaataatc aaaataattc
     3001 ttcaattcca ggaaatgaga ttcaaatttc agatagttat ttgccctttt aagtgaaaaa
     3061 taattttgtt ctatacctga tttcttcagg tatagaacaa aaaataaata gagatgaggc
     3121 gattttttat gaaaatcatt gacagttacg atcaacgaac catgcaatat ttgggctcgt
     3181 ttgattatac agatgaaaat attattaatt atgttgcaag tttacctcct tatcaaagcg
     3241 ttcgcttggt agattttgca agtgatgaat tggtattaac aacgatgggc aactttttag
     3301 attatgtcgg agacaaaaaa tggttggaaa aaattttgtc taaattgatt gcgaaacaaa
     3361 caggagaaga acccataaat gcgattcaat tgttcaatcg atatgaacaa gaaaacaatg
     3421 attaaagagg agagaaaaaa tgaaaagatt aaaacgaagt atttttattg tgctagcatt
     3481 gattgatttt gttgggacag gttgtgaagc aattgacgtt caaaaagctc ccttatcgga
     3541 aataaaacaa gcaataacaa aacaagatga agaacaaccg ctaaatacac aagtagaaat
     3601 agtgaatcaa aataaaccga gtttttctga agaagatttt tcgttagaaa atggaaccag
     3661 gcaatttttt tcagatttgg atcgtttaaa tcgggtaggt gtagcaagtg cacttttgca
     3721 taaaagcagg atgccaacag aagaacgaga ccgattatac attaagccaa ctggctggaa
     3781 acaaaagaaa atgaagaatg gcgattatct ttataatcgt tgtcatttga ttggctatca
     3841 acgtgcgaca agtttcccca taagtatgta atacgaaacg ggaggattaa tcttaagatt
     3901 aatcacaact aacctatttg acagttggaa tgaaagctat catgtctgct gaatcaactg
     3961 gtctgatact cctacatgca ataaagtcta ctaggaagtt attgtatgaa gctaggtaaa
     4021 ggcggcagaa caatttctaa gtgtggaatc atatgaagtt ggatatgccg ggacgctata
     4081 aattactcag ggtgagactg tttttaatta aactgacgaa cttgcgaatg tacgggtcta
     4141 taaccgactt atattcgaaa gatataagat tgcgaatgca aagaacatta tcgaaaagta
     4201 agaatgttgg tcatgaaatt cgagatggaa gactagcggt gtatctgtca agagttggta
     4261 aaacatgttt gattgattca gatgagagtg ggaatgcgac aaaacgtttt acagaagaaa
     4321 tagagcaact agcagaactg aatatacgat atttttaaat actttagcgg agaataaaaa
     4381 aatcctctac agaaaactac ggagggcaac atgtgctatt gtttcaacaa tacattttta
     4441 atttttacca taacaaaaaa gtacttgaaa aaaaacagaa atatgttaaa ttaaaaatga
     4501 aaaaatggaa gcggttactt attcttttga atctaagata aaaattcaaa agaataacat
     4561 aacccaaata tgattatact tgagattcta aatatttatc gaaaaggaat ttgttttaag
     4621 gaaaaatgat tctatggacg gttattgatt tatatttacc aaatggctaa taaaatagta
     4681 tttcttatat tgtatagttt ttgtttttat gtgaaaacat gagcaataga attgtactag
     4741 taggataagg atggatggaa aatgaaaaaa actatttttg ttttagcgat ttttatatgt
     4801 tttgggctac ttttggtagg ctacttgttg tttaattcta atgaaatgag aactatttct
     4861 acgaatgact tgttaaaaaa aagtgaatta aaggataaaa aaaaagaatc tcttgccttt
     4921 ataatgttta ctcaagaaga gtgtgagtat tgtatagcaa taaatccaat catagatagt
     4981 gtaaataagg aaactttgaa taagataaag aaatatgata tatctgtaga ttttcaatct
     5041 aaacaaaagt gtaaaattaa gtttgttccg actattgttg catatagtaa gaataaagaa
     5101 gtggggagaa ttgacaatga attagataat ttgtatcgaa aaaagcaaga tggttcaatt
     5161 gtaatagaca agaaaaaaac aaagacattg cttatagatt ggatgaaaaa aatggataaa
     5221 caaggagaat atttgtatgg agaagattaa acagacattt ccgttaatta ataaatattg
     5281 gaataagaaa aaaattttta tattttttat tacgtttctg ctgtcatctt tattttctct
     5341 aattttcccg tatctattta gtaatgttat tgatttatat ttagagaatg agttatcaca
     5401 gaatattgtg cgaatagtaa cgattcttat atttcagtta tttttagtct taattaaaga
     5461 agtgtatgga tatataaaaa ctatttatgc atttgaagtt gatttatcaa tgaaaattga
     5521 ttttcttaag gttttacaaa aaaaaaatct ttttttttcg caaacgaagc attcaggaga
     5581 aattcaattt aggatgtttt cggatatttc tactatcgta aatactatct ttattttttc
     5641 tgttgaatta cctgtgatag gcgttttagt tcttgtagcg gtatttatta taagtaaagt
     5701 gaatcttatt tttctgattt tttcactaat aatgatggga gctatggtgt tacagtataa
     5761 tttatttcaa ttaccgctta agaaaacaat cgagaataca aaaagtgtta atcaaagtat
     5821 tacaggagaa ttgaatgcac attttgatca attagaaatt ataagcgcat ataattttga
     5881 gaatatctct attttaaaat tcgattctag atttaaagaa ttatttaatg ttttcaaaaa
     5941 acaaaataaa ttactaacac tgggaagctg tttggcacag gttattttcc aattatggat
     6001 tggaggaata tttcttattg atgttatttt attagctaaa ggaacaataa gtattactaa
     6061 ttttgtaata gtatttatgt acgctaacat ggtccctact ttatggacta ctttaattag
     6121 tattatttgg aaatggcaag actattcagt gtcattaaat agatattacg aatattattc
     6181 aaataacaac aaagtggctg gcacaaaaaa aataaataaa ataaattcta ttactggtca
     6241 atcattaaat ttttcatata acaaaagaaa ggtattaagc aatataagtt tttcaactga
     6301 aatgaataaa ctattcatga tatcaggaga aaatggaaca gggaaaacaa cactgatgaa
     6361 attatttact aaacagttgc cggttagtaa taatcaatta ttcgtcaacg ggatagatat
     6421 taatatgatt gataacgaat cattaagaag gttaatttca atcgcaacac aaataccata
     6481 tataattaat agctccgtga aggagaatat tttaatgggg actaaaaact atgataaaaa
     6541 aaaattcagt aaattaatag aactattggg aataagtaaa tttatgacga aattaggtat
     6601 tactttggac gacaatattt atgatgaaaa aatattatta tctggaggag aaaaacaaaa
     6661 aataagttta gctagggcgc ttattaaatc agcttcagtt attatattag atgaaccatt
     6721 tagaaattta gataatgaaa ctcgcgataa tttgagggat tatatactaa atacaaaaaa
     6781 agaagtttgt tatataatta taactcatga tcacattcta gataattttt tggattcaaa
     6841 tgatgaaata ttatatttaa ataaggagta tgataataat gaatagcaaa aaacttcaga
     6901 tgggtgaaat tgttagaggt cctagtcaaa tatctattga tgttacatat aaatgtaatt
     6961 ttagatgtaa acattgttac aatgcgagtg gtggagacaa ttgttttatg gataatgagt
     7021 tgactgatga tgaactgtta gaacttacaa aagatatatg taatttaaag ccatataata
     7081 tttgtttctg tggaggagaa actttactga gaaaaaaaat agttttatct tgtgccactt
     7141 tattaaaaga ggcaggtatt cctaatattg ctattgttac taatggatca ttagtaactc
     7201 aggatgttgc aaaagaatta ttcaaagcag gtattaatcg atttcaagta agtttagatg
     7261 gaaaaaatgc tagtagccat gaagcactta gattatatcc aaacagtttt gaaaaggcaa
     7321 ttaatgctat caaattattt agagaattgt ccgaagaagt ggatgttgct ttttgtccta
     7381 ctagttttaa ttacaatcaa ttacgagaag tatatcaact agtaaatact cttaaagtga
     7441 agtctttaag aattcaacca tttatgctta gtggtagggg aatgcaaaat agggaaatag
     7501 tgccaactgt ttctcaatat agaaaactag tttctgatat taactatatt aaaagaaatg
     7561 aagattatac caagatagat tggggagatc caattgatca tatcatccga cacagaactt
     7621 tattgaaaga tttttcgaat tttgtttcta ttaggtcaga cggaggatta atggcatcac
     7681 cttacttgcc aattcaagtt ggaaatatta aatgtcacag tctttctaaa tattggcgag
     7741 caggacttta taaagttttt tctttagatt tagttcaaga tatagctact caagtttctt
     7801 caatacaaga tatgagtaaa gaattaaaaa aaggacatat taaatggctt aatgaagata
     7861 tagaaattga tattctcgat gaatacaaac atgatatttt aatttaggtt tatcattgga
     7921 aagtttagat aaacaaagta cttttagaaa ggagggaaaa aaatgatttc tgaacctcaa
     7981 atgaataata tgtatgaaag cccaactata atgggagctg gtgaagctgc gccaagagct
     8041 tgttcagcaa ttatggtgtg tgtgttatta gcatctgcag gagtaggtat ccaagcagga
     8101 gcggtgtata cttatgctgt agttgctaca gtagctgtag ctgcagccgc agtttggaaa
     8161 acagtaggat tagttggtta ggaattaata ggagactaac tgtctcctat tgattccaat
     8221 gattaagcta aggaggaatt ttaagaaaat aatgaaatac aataatttaa aaccgaaatt
     8281 acttaatgag aacatagaat atataagaga agaaaattat ggatatttaa ccatagttcc
     8341 caaaaatgga actgaattga aagataacgt tttcaatcca acagtaagat atatattggc
     8401 taactgtact ggaaaaaaca gtgtaaaaaa aatagcgaat ggtttaaaaa agaagtttcc
     8461 tagtttagaa tatgaaaaat tctatcaaga tgttgtagga actattaaat ttttcaaaca
     8521 ctataatatt attgatatag gagattatag tatggaagaa aagaaaatat ataaaattga
     8581 acaaaattta gaattagaat tactaaatga atcacattat gaagaaattt ataattttat
     8641 cacagatcat cctgaaagta tgtattttca tgaagatatt tatcatcaag gaaatagtaa
     8701 agaattagaa gttgtcctta gatataattt attttcgttt acgagtgaat tttacttttt
     8761 aagacataaa gagagaattg taggaatggt ttctataatt gttaatccat ttcaaaactt
     8821 atcctacagt atagtaaatt acctcatatg tgagcctgca tatactacga ctattttgac
     8881 aacagtatta gaaaatatta acgaagtttc aattttagaa gtaaagaagc tagtgattca
     8941 tttgctaaat tcaacagaag atgaatcact aatcccctat ttaaaagatt cacatttttt
     9001 taaagaagcg gtactatcta aagaatatag agagcatgat gttttgagat acgcttacat
     9061 ttattaaata aaatttctga tagtatttta ataattcgga tcatatcata agatatagga
     9121 gggaaaagaa gtgattttat tgattttgtc tctcgtatta ctagttgtat ctaatctaat
     9181 gaaaaataat cctcctacaa aaattaattc tttttatggc tttagaacta aaaagtcaat
     9241 gaaaaatgaa aaaagttggc atttagcaca aaaattgttg ggggattttt caaaaaaaaa
     9301 agttatccca gtatttttga tgggtattgt ttttttcagt atagagattt atagtttagc
     9361 tattttaaaa aatgaaaatt ttttcgttag tgtattaatg ggtgaaagtt taatcctaat
     9421 actgattttg tgtttaattg ttcatgaagt gaataaacaa ttgtgaatta aaaaaataaa
     9481 aacctagtat aatcacttta ataaattgtt ggtagaaggt gatcaaaatg ggagtaaacg
     9541 aaatgatgaa agtcagattg agtttttaaa tgataccttc ctcagaactt caaaacgaat
     9601 tcaaagattc gttgacaaat cttgagcaag atcatttaaa aaaatatttt ctcttcattt
     9661 gacgaaaaat gtttagaaat cttatataag tgaagataaa gtaattggtg taattaattg
     9721 tagagaatag cctttgagca attgaagcga tacaattatt tagaaaatga acttcctcca
     9781 gcatactaca tgattcctta cgggacgatg ttaaagaaga aagcaagaaa ttttagaagt
     9841 attagtagat aaactaatgg gcttttctca atttaaggga gtagcccaaa tgtttatcaa
     9901 atatggtgca gatacagtag caaccatgtt tacagaagaa tatcaagtag actattatga
     9961 agtaatagat aaatgtgtac attttgttta tgaaaaagtg tggaatttta tgaattttgc
    10021 gataagtgaa gaaatcttac gagtacaaga aaacagaaca ggaatattta ccagagacag
    10081 aaagcttaat ttgattgcgc attatatacg gaatcgctgg tcatcaatac caaattttaa
    10141 ttataaaaaa taattatgaa caatctttta tacaatatac acaataaatc aagtagtatt
    10201 tatgaaaaaa gacttcaaaa acagaatgat tttctatcag tcttagaaat tatagagcta
    10261 aaaattttgg tcacgcgtag aaacgcgttt tttttgtttt tttgatagta aagaacgtac
    10321 aaaacaatga gaatttaaaa gattgtacta ccagtttttt taccttttca tttgagttta
    10381 gagtaagtaa aatattacta taaaaacaca tattcttgaa gaaatctttt ttttgctgta
    10441 ttcatttaga ttatgataga gaaaatacgt gagaggggga cctgggatgt ggctatctga
    10501 tgaaaaaatt cagtggcgta gctatcagaa gcgtatattg actaactttg ctacttattt
    10561 aaataacaat tcagtccatc tagtggctcc accaggggct ggcaagacct ttttaggaat
    10621 tgagatgatc cgacgtttgg attgtaaaac cttaattttg gttccttccc taatattgaa
    10681 gaaacagtgg ttaacaacaa tcgaggaata tttcttagta aatccagcag atagttgcta
    10741 tctttctggg gcgattgaac atcctagtca tattacagtg gaaacgtatc agaccattta
    10801 caaccgactg aaggaggagc caacttatgg tgatcagctg gagctgttag ttttttgatg
    10861 aagctcatca tttaaaaaaa agctggggtg aattgttgat tgacttgaag aaaaaacaac
    10921 cgaatctgtt gacgatttcc ttgacagcca cgcctccgat tgatagctct cagacggagt
    10981 ggaagcaata tattgagtta aaatggcctg attgacgaag aaattagcat tcccgagttg
    11041 gtaaaggagg aggttctatg tcctcatcag gattttctat atttgattcc ggctaatcag
    11101 gaaactactc agcattattt taccttttta gagaaacaaa atgagctggt ggaagcgtta
    11161 atggaaagcc aagagacggt ggattatttg ctttctttag aatttgttca gcatccgctt
    11221 gagcattcga aggaaattta tgaaaatttt gagctgttca ctagtgcatt aatttacttg
    11281 aatcatcata actatgaatt gatacccgaa cattggcagc tattaggcag ttctaagcag
    11341 caaggtaaaa aagcttcagt tccatcttta ggtaaaaaag agctgatcac tatttatcag
    11401 cagatgtttg aagatcaacc ggaattgcct attttttatc aattgaaaaa agctggatga
    11461 ttgtttgatg agtcgctgca tttgtttcca gagtatccag acactgttgg tagtcaggcg
    11521 attcctgaga aaaagcaagc agtttgtcaa attttgttaa aagaagaagt tcagctgaag
    11581 caagaactga cagcggtcat tttgatggat cgtatttgca aggaggtatt agcaggcagt
    11641 gatggtacct tagcttatgg cgtggttcct ttgtttgatg aagttgttcg tctattaggt
    11701 aacactcagc aaatcggtgc catctgtgga gagttcttac tgttaccgaa aatgctagta
    11761 actgttgagc tggcagaact agcgacgggt atgaatgaac atccttatat gtcgggttat
    11821 ttttatttga cggtggatga caaaaaccgc caagcattga tgcaagcagt tactcgccta
    11881 ttaaccgaaa agaagttgaa tattttggta ggaaccgtta gtttgctagg agaaggctgg
    11941 gattgtcctg caattaatct gttgatcttg gctaatcaag cttccacttt tgtgcaaaca
    12001 caacaactac gagggcgggg cttacgccta ggtgcacaaa ataaagtaac gaacatttgg
    12061 catttagcga ttggattacc gggagttcct ctgtctgaac agcctgattt gaaaagaatg
    12121 ctacggcgtt tgggcaatat ttcaggtctg acctttgaat cttcaccaaa aattgagagt
    12181 ggtggcgatc gctttgcttt accactggaa tatacgacca ccacgatgga aacttatact
    12241 cagtcgatgt tctatttttc ttgtgaacga agtcggttga aagaacaatg gagtactgct
    12301 ttagctgatg gctggcacct atctatgcca atcgtcgtta ggaaagtcga tcaggtaaag
    12361 caaaaacaag aaacttcatt gccgatagta gtaaaaaata tagagcctct aagcttctgg
    12421 tcagctatat ttaaaggtaa cttgctggaa tatttccaaa ttcgtaggcg acaaaagcta
    12481 tgggaaaagg aatgtgcccg caaaaaaagc ttggccatat gcgttcaaac gacgttgcag
    12541 cgccttggtg aagttagtga tagaaaagta atcgtagcgg ctgatcagca ggaatttaga
    12601 tgctatcttg agaatgctgg ctatcgagaa ggacagcttt ttactgaagc tatgagggag
    12661 cttttagcac cgctggagaa tccgcggtat ttaatcgaag ggaaaaagat agttctgacg
    12721 gtaccagaac gctttggccg aaataagcaa atcgctaacc tttttctaaa cgaattaaat
    12781 caatcgtcgc tgcccaaatg tcagttggtg tatactagaa atcctgaagg acgccaaaag
    12841 ctagtgactt atcgaatgag gcaagtcttc cagacccgcg atgaagtaat tattcaaaaa
    12901 aacatttgga cgtaagttga tgaaaataaa ttttgctaga gtagaacaag tccgagaatt
    12961 aagttgctac ttaactgata atgccaagga caggagaatc ttttcttgac gactgaaaga
    13021 aactatttga cttatataac ggtgtgtgat gaatatttgc aaggcattct cttaaaaagt
    13081 actcgtaata gtttttttga aaacaaaaca gtactttatt aataggactg acagagcgat
    13141 ataagaacat ctgcagacag aggtgaatgc ttttctcatg atcgatgaaa caatggtact
    13201 tcggatattc ttgcttttag tttgaaccgt tgtgattttc tttgattcct aaaagtagtt
    13261 ttatagaatg aaaatcaagc atacggaatg ctcctttgat tgtgttgtgg tgattcaatc
    13321 taatggagct tctatatgtt tttttatttg caaaaagaac cgatagaata tttttatcag
    13381 tcttagaaat tatagagcca agaaatttca agcacacata aaaacgcgtg ttttatgcat
    13441 tttatgagca aaaataacta aagaccatta cgtaaaacat aggtaaatca gcattcaatc
    13501 tcaaattatg tgatcaaatg ttgatattat ttgattataa gttttctttt gctattatca
    13561 gtctgcaaat aaacagatta tagagtatga atgtagatag ttcaatcttt gtttacgacc
    13621 tcacatgttt ttttactcaa gagaaagtaa tataaagtga ctatatttga cagaacttta
    13681 cataaaaaaa gtagttagtt ttctgtagta atcagaaatg taaataaatt aaaaaaaaga
    13741 aatttagcag ttatatattt ttaaaatatt ttgggtgagt agtcaataca acattttgtt
    13801 catcaagcat taaatgaaga tgcaaggatg ttaagaagtt agcttcttca gggtcttgca
    13861 tggtcaaggc tttaaatagt tgatgatgtt gttccataat tttttcccat tttaatcctt
    13921 ccgtttttac tcgtaaccaa cgaaatcgtt ccaagtgcgt attattatct tccaaccatt
    13981 gccatacttc ttcccgttct gcaatttcaa aacaagcttt atgaaataaa ttatcattat
    14041 gaaaaaaact acgagcattc ttttgactaa cagctttttc ttgttctgca attatagttt
    14101 ctaaaacttg tttcttttga ttattcatct tggcacaaca ctctagcata atttgtcgtt
    14161 ctaaatgttc tctgacaaaa cgagcatttt ttgctaaatc taagtcgatt aatgagatat
    14221 aatttccact ttgaggaatt gtatcaacaa gtccttgatg acggagttga atcaatgctt
    14281 cacgtattgg agtacgaccg atagaaagcg tttcttccaa ttttttttct gatacttttt
    14341 ttccaggttc taagtcagcg tagataattt gctgacgaat agattgataa gcttgatttt
    14401 ggagactagt tttaattgtc atatttaatc cctctcaaaa tttcattttt gaatttgata
    14461 ttttagttct atgctcgtta tataatatca aatttcaaag taacatacta gctattaaaa
    14521 aaacaaaaaa tactcaaata agatcaaaga gaaattaagc tttaatttaa gttagttcta
    14581 aaaaatatat attggtgtgc ttttactaaa aaaagacaca ataattctga catggtagaa
    14641 aaaagaatat ttttagtttt ttcttattga cagcgattac attcgatttt atactacaat
    14701 tgtaacggaa actaacatat cagtatggag gggtaaatat ggttttgatt aaagactggc
    14761 aagattctat cgaaggattg aacaaaatgc aagttcaact accgtatttt aatgtcgaag
    14821 cggtcaaaca agaaggtcag aaacatccag tgtggcttca ttttggtgga ggaaatcttt
    14881 atcgaggatt tcatgcagaa attgctcaat gcttagcaga tcaaaatgaa ttagaatccg
    14941 gagtagttgt gtgtgagaca tttgatgaag aagtagtaga aaaagcttat catgactaca
    15001 acaatgatat tttggagatt gttatgggtg aagatggtga actcaaaaaa agacttttag
    15061 cagtaacagc tgatagttta tattgcaatc caaagaatat aacggactat caaagagtgg
    15121 ttaaatattt tgaagatcct acattacagt ttacaacgtt tactattact gaaaaaggat
    15181 acagtttaaa agattctaaa ggacaatttt tttcggtagt ggaagcagat ataaaatctg
    15241 gacctaccaa tcctcaacat acgatgagta ttgtgacatc cttgttgtat agcaggttta
    15301 atgctggaga gttaccgatt gcgatggttt ctactgataa tttctcacag aatggtcaac
    15361 gttttcaaga tagcattttt atgattgcag aagcttggca aaaaaaagga tacgtttctg
    15421 aaaaatttat tgcttattta aaggataaag aaaaagtgac ttttccttgg agtatgattg
    15481 atagaattac gccaaatcct tcggaaagtg tcaaaatgga tttattgaag gcgggtttcg
    15541 aagacttaga tataattcat actgcgaaaa ataccaatat cgctgctttt accaatactg
    15601 aaaaagtcca ttatctagtc attgaagata gttttccaaa tggtcgacct aaactggaag
    15661 cggctggtgt gattatgaca gatagagaaa cagttgataa agcagatatc atgaaggtta
    15721 cgacatgttt aaatccgctc catacggcat tagcaataac aggttgtttg ttggggtata
    15781 aatcaattca ggcagaaatg caagatgatg atttggttgg cttaattaaa gggataggtt
    15841 atgtagaagg cttaccagta gttgaaagtc caggcgttat tgatccaaaa caatttattg
    15901 atgaagtaat caataaacgt ttgcctaatc cctatattcc agatacaccg caacgaattg
    15961 caacagatac ttctcaaaaa gtagcgattc gctatggtga aacaattaaa aagtatgtcg
    16021 aaaaagacgg aaatgcagaa aatctaacat ttattcctat cgctattgct gcttggttac
    16081 gttatttatt ggcagtcgat gatcaaggaa aaaattttgt gccaagtcct gatcctttat
    16141 tggtagagtt gcaggaaaaa ttctcagtat tgaaattagg tgaacaagat cctgcaaaga
    16201 ttcattgtgc ggtacaggaa atcttagaaa ataaggaaat atttggttta gatttatatc
    16261 aagttgagat aggaaagaaa attgaaaaaa tcttttgcga aatgctagta ggtcctcaag
    16321 cagttcgtaa aacaatcaat aaatatataa cagaagttgg aggaaagtaa ctatggaaat
    16381 gacttttaga tggtatgggg caaatgaaga aaaaatctca ctacaagaaa ttcgtcagat
    16441 cccagcaatg aaaggaattg ttggaacatt aatggatata ccagccggag aggtctggcc
    16501 gagagaacga attaaagctt taaaagatga agtcgaagcc aatggcctta ccttgaaagt
    16561 tattgagagt gttaatgttc atgatgatat caaaattggt ttgccaacac gagatcaata
    16621 catcgagaat tataaacaga cgattcgcaa tttggcagaa tttgggattg aggttatctg
    16681 ttataatttt atgccagtct ttgattgggt aaaatcagat ttggattatc atttagatga
    16741 tgggtcttct acgttagcct atatttcaaa aaatattcct actgatccag aggaaattat
    16801 tagaaaagta gaaaattcta ctaataattt tgaattgccg ggttgggaac ctcatcggtt
    16861 atcacaaatc aagcaattat tagaagccta taaagaagtt gatgaagcga aattgcgaga
    16921 gaactttgca tattttctca aagcaattat cccgacatgt gaagaagttg ggattaaaat
    16981 ggcagttcat ccagatgatc caccatattc tattttcgga ttacctcgta ttgttaaaaa
    17041 tcgtgacgat ttagaatggt tgtgtaatgt agtggattct ccttctaatg gtattacgtt
    17101 atgtactgga agtattgcag aacgaccaga taatgatgtc tatgaaattc tatctgaatt
    17161 ttgtcaacgg gatcggattc cttttgcaca tgttcgtaat atcaaatatt taagtgataa
    17221 tggactcgat ttttatgaag cgccgcataa atcagaatat ggttctctag atatgtataa
    17281 gatattaaaa acaatgtacg ataatggatt taatggctat attcgtccag atcatggtcg
    17341 gatgatttgg ggagaaaccg gtagacctgg ttatgggtta tatgaccgag ctctaggtgc
    17401 agtttattta aatggtattt gggaagcgct tgaaaagggg gcgagtaaat aattatataa
    17461 tggagtctat ttagaagaaa aattttctct tctaaaaact aagggaagga aaggagtgct
    17521 taaatatgtt tttagataag aactttttat tagagaatga aatagctaaa agactttttc
    17581 ataaatttgc agaacaccaa ccaatcatcg attatcattg tcatttagac ccagcatgtt
    17641 tgctagaaaa taagcaatac aagaatttga ctcaggtatg gttaaatgat aaaggagctg
    17701 gagatcatta taaatggcga ttgatgcgag ctaatgggat agaagaagag ttgatcagtg
    17761 gtgcaggtaa tgattatgaa aaatttttag cttttgtaaa aacagtggag aaggcgccag
    17821 gtaatcctat ttttgaatgg tctcatttgg aattacaacg ttactttgat attgatttga
    17881 caatttctga gaagaatgca ccagaaattt ggcgacgtgc caacgaaaaa atccaaacgg
    17941 attcctatcg cccaaaagaa ttgctgaaga tgatggatgt gaaagccatt tgtacaacgg
    18001 atgatcctac agatgacttg cgatatcata ctgagctttc taaacaggat aattctttca
    18061 aggtattacc tactttccgt ccagataagg tctggagtat caaatcatca ggattttcga
    18121 actatatttt aaaacttagt caagcagcgg gtataccaat caaaaacttt aatgggttga
    18181 agcaggcatt gagccaacga gtggattatt ttcatcaaat tggctgtcga ttagcagatc
    18241 acggtttgaa tacttatttt tatgaaatag cagatgaaaa aatactcgac aatctgttaa
    18301 taaaagcttt agcaggaaaa acggatttcc atttatcaga aattgcacaa tttgttacag
    18361 gtattcaatt acatttgatg tctgagtata caagatatgg ttggacgatg caaatgcatt
    18421 taaatgcttt aagaaataat tcaagtatat taaaggaaca aattggagta gatgtcggtg
    18481 gtgattcgat gggggatcaa gctagtttga cacaaaatgt tgtagcattg ttcgacactg
    18541 ctgaaaagag tgggtgctta ccaaaaacaa ttttatattc attaaatcca accgattggc
    18601 ttccactagc aacagcaatg caaagcttcc aagggggtat gattcaaaaa atccaattgg
    18661 gatgtgcttg gtggttcaat gatacagctg aaggaatgcg tcaacagttg actacgatgg
    18721 cgcaacaaag tttgttgtcg aattttacag gtatgttgac tgattctcga agttttctaa
    18781 gctatcctcg ccatgagtat ttccgtcgag tattatgtca attactaagc gagtgggtta
    18841 aagcaggtcg aattccagat gatgaaatgt atcttagtca gatagtggag aatatttgtt
    18901 atcgtaatgc gtataattat tttaattttt ttgaaatcga aagtagaaaa taatacaaaa
    18961 aataaaagct taaatttatt tattgtaagt tagtacaact agctagagta tgtatccata
    19021 aaagaaggag atagatatga aaaaagtaga aattttaagt aagttagaag cggctggagt
    19081 cattgcggtt gtacgtggaa aaaataaaga tgaggccatt aaagcaataa atgcaattac
    19141 tgcgggtgga attaaaggga ttgaactaac ttttacagtt ccgaatgctg aagaaataat
    19201 caaagaaatt gctaaaaaat atgctgatca aaaagatgta ataattggag ccggtacagt
    19261 tttagatgcg tttactgctc gtattgcaat tatggctgga gcagaatata tcgtaagtcc
    19321 atcttttgat cgacaaacag cagagatttg taatttatat caaattcctt atttaccagg
    19381 ttgtatgaca attactgaga taaaggaggc acttaccgct ggagtagaaa tcattaaact
    19441 atttccaggt tctgcttttg gtccatctgt tgtaaaaacg tatcaagctc cattaccata
    19501 tgtaaatata atgcctactg gcggagtaag tctagataac ttagaagagt ggtttgaagc
    19561 aggcgttgtt gcagttggtg taggtgggaa tttgttggct cctgttgaaa aaggtgattt
    19621 tgaagaagta actcgaatag ctagtaaata tatagataag ttttatgaga taaagaatta
    19681 ttaggagaaa aaataaataa tactataaaa tgtaaaaaat aaatactaac tctttatact
    19741 tcagagtata gtacgtaagt tggcagttca acagaatgaa aacgcttaac aataggaagg
    19801 agcgcaaatt atatggaaag aaaatttggt ctaagagaca aaataggata tatgtttgga
    19861 gattttggga atgatttcac tttcattttt gctagttctt ttttaatgat tttttataca
    19921 aaagtattgg ggataagcgg agctaccgta ggaacattat ttttagtagc aagatttatt
    19981 gatgcagtta cagacataac aatggggcgt attgtggatg gatcaaaacc atctaaaact
    20041 ggcaaattta aaccgtggat tttacggatg agtggtccag tcgctttagc aagtttttta
    20101 atgtatcaaa catcaatggc atcagcttct atgagtatta aagttatata tatgtatgta
    20161 acatatatac tatggggaag tattttttat acatctatta atattcctta cggttcaatg
    20221 gcatcagcta tcacttctgt tgcagaagaa aggacagcgt tatcaacatt tcgtagtata
    20281 ggagctacct tagcaggtat gttcattggc gtgttaacac caatttttat ttatacaaga
    20341 gatagttctg gagctcaaat tgtacgtggt ggatcaactt ttacaattgt ggcaggagta
    20401 ttttcagtgt gtgcagttat ttgttacgta atatgttata aattaactgt agaacgggtt
    20461 aaaagtgatg aatcgcagtt aaaagtttca ttgttgaaat ctttaaaagg attagctaaa
    20521 aatagagcgt tgctttctat aatagctgca gctatttttt tgttgcttgc tcagctttta
    20581 acaatgagta tgaacaatta tgtatttcca gattattata ataatgcaca gggaatagtt
    20641 ttaatgaact ttatcaatcc tattttgatg ttggtacttg tttcacctgt aagtttaatt
    20701 ttcagtaaaa aatacggtaa aaaagaattg gcgtcagtag ggatgttctt ctctgctatt
    20761 gtatatttac ttttattttt gataaagcct agtaatatgt atgtattctt agttcttact
    20821 tctgttggat atatggggtt aggtattttt aatacagtca tttgggcgaa tattactgat
    20881 gtaatcgatg atcaagaagt gaaatctggc caaagagaag atggaactat ttatgcagta
    20941 tattcttttg cccgaaaaat tggacaagct ttagctggcg gtgtcggagg ttggactctt
    21001 tctataatag ggtatgattc gttagcaaaa gtacaaagtg gagaagtttt acaaaagctg
    21061 tataactctt caacgttgat tccagcagtt tgtttctttg ttgtaggatt gattttattt
    21121 ttcgtatatc cattatctaa agaaaaagta ttagaaaatt ataatttgct aaatcaaaaa
    21181 cataactagg agaaaaaaca tgaacagttg gatattttgt attgattcag atggttgtgt
    21241 catggataca atgacttata aacacaatct ctttttcggg ccaattgctg cagagatttt
    21301 tgatgttcat catagggaaa aattcctaca ggattggaat aatattaatt tatattcaga
    21361 gactcgtgga ataaacagat ttttaggttt agttaaaaca ttagaaaaaa atgatgtacc
    21421 atcgattaat cgattaaagc actgggtatc tgtaacaaaa gaattgtcta atccatcttt
    21481 gaagagggaa atagaatact ctcagtcaga ggatttatta aaagctttaa aatggtcgga
    21541 agaagtcaat cgacagatta gtcaagctaa aggattagat aaaccctttt taagtgctag
    21601 agatactata aaagctctga aaaaatatgg gaagataatt attgtaagct cagctaataa
    21661 ggaagctatt caagaagaat gggaaagaca tgaattatta tccttagttg atgaactgtg
    21721 ttgtcaagat aaaggaaaaa aagaagacat tattcgaagt gtaattgaaa atggttgtga
    21781 tttggataag atattaatga ttggtgattc tcctggtgat ctagaagctg caaataagaa
    21841 taaagttttc ttttatccta ttttggttaa taaagaaaaa gaatcatggg aaaacttaag
    21901 aactgatatt ttatttaaat gtttatcggg agagtatata gatattgaac aaaaatatat
    21961 agatactttt tggaaaaatc ttactaataa gtagaggaga aagaaaatga ctataaaagt
    22021 agacttacga aaaaaaccat atcatttaga tgaagatgct atttcttgga ttgaagagac
    22081 gattgatgct atgactttag aagaaaaaat aggtcaatta tttgttaata tgggatctag
    22141 tcgtgataaa aattacttaa gtagtatggt caatcagtac catattggag ctgtacgata
    22201 taatccagga actgctgatg aggtatatga tcaaaactat atcctacaaa cagaaagcaa
    22261 aattccttta ttaattgctg ctaatacaga agcaggtggt aacggagctt gtacagatgg
    22321 taccgaagtt ggtgtagaag taaaaattgg tgcaacaaat gatgcgaaat atgcttatga
    22381 attagggaga gtttctggaa ttgaagcatc ggctattgga tgtaactgga gttttgctcc
    22441 tattgtagat attaatagaa attggagaaa tcctatcatt tctagtcgat cttttggaca
    22501 aagccctgat aaagttttag aacttagttt agcttatatg aaaggtattc aagagagtgg
    22561 aattttgcct gcagctaaac atttccctgg tgacggtatt gacgaacgag atcaacatct
    22621 atcttttagt attaatagtt taagtacaga agaatgggat aattcttttg gaaaagtata
    22681 ctcaggctta atagaagctg gattaccttc aattatggca ggtcatatcc atatgccagc
    22741 ttattcaaaa cattttaatc ttgatctgaa agatgaagat ttacttcctg caactttatc
    22801 aaaagaatta atcactgact tacttagagg aaaactcggg tttaatggat tagtagttac
    22861 tgatgctagt catatgttag gaatgactgg ggcaatgaaa cggtcagaat tattgcccac
    22921 ttctattgcg gctggatgtg atctattcct attttttaat gatccagatg aagactttaa
    22981 ttatatgatg gaaggttatt acaaaggaat tatttcagaa gagcgtttac gtgatgcatt
    23041 aagaagaatt ttaggcttaa aggctaaact aggtttgcat aaaaaagaaa agaataaaat
    23101 tttgttaccg aaacaagaag ctatggaaaa aattggttta attgaaaata aatcaatttt
    23161 taaagaggta gctgatcaag caattacact agttaaagat aaacaagata tttggcctat
    23221 tgtaccagaa aaatataaaa ggattttact ggtagatgta catggggtaa aaggtgggtt
    23281 tggtgaagta gttgctggaa atagacctca ggccattgat ttattgaaac agattttgtc
    23341 agcaaaagga tttgaagttg aaaaatacat ttcacctgaa gaagaattga aaggattatc
    23401 gcaagaagaa aaaattaaaa atatagctgg aatatatgct gctaagagac caatcaaaga
    23461 tttaactgaa aaatatgact tgattattaa tatagctaat gttaacccag gtacagttca
    23521 aagaattgtt tggccagcaa gtaaaggtac tccggatatt ccattttata ttcatgaaat
    23581 tccaacaatt tttgtatctg tacagtgtcc gtttcattta gctgatgtgc ctcaagtaaa
    23641 aacttatatc aatacttatg atggaaaaca agaaacttta gaaatattag tggacaaact
    23701 aatgggcctt tctgaattta agggaataag tccaatagat gcttattgtg ggttaaagga
    23761 tacatttatt taatatttaa attatatagg agtgatttac atgttatatc ccattatgac
    23821 agagacgagg caagtaattg atttaagcgg tctttggaga tttaagttag atacaaatga
    23881 tgacttacaa gaagaacttg ctttagaacc aatggttgaa aaagagagtt atccaatttc
    23941 agttccttct tcttataatg atttatttga aacagagaac attagaaatc atgttggatg
    24001 ggtgtggtat gaaaaagaag tgattcttcc taaaaaatta ttagaagaaa gattagttct
    24061 tcgttttgga tctgcaacac atgaagcaaa agtatttttg aatggaaaat tgattaatca
    24121 acacaaagga gggtttacgc catttgaatc agaaattaat cagtttataa aagatggaag
    24181 taacagaatt acagtggctg taaacaatgt tattgacgat agcactttac ctgtagggat
    24241 tttgaaagaa acgatacagg aagatggaga cataaaaaaa gaaaatttgg ttaattttga
    24301 ctttttcaat tatgcaggca ttaatagacc cgttaaattg tatacaactc cgaaagacta
    24361 tataaaggat gtcactatta atacaaaaat aattgattca gatgcaaatg tagaatttaa
    24421 tatcgataca gaatggaccg gagaaattct aatagcaatc tatgacgaag aagctcaact
    24481 tatagcagaa gctaagggga aagaaggagt gattcaactt aagaacccaa cactttggga
    24541 gcctctaaat gcttacttat atagtatgaa agttagttta attaacgaaa ataaagtgat
    24601 tgatacgtac acagaagaat ttggcattcg tagtattcag gtttcgaatg ggaagttttt
    24661 gattaataat aaaccttttt actttaaggg atttgggaag catgaggaca cgtatattaa
    24721 tggtcgtggt ttgaatgaag cagtaaatat caaagatttt aatttaatga aatggatcgg
    24781 tgctaattca tttaggactt cacattatcc ttattcagaa gaaattatgc gcttagcaga
    24841 tcgagaaggt attgtagtta ttgatgaagt acctgcagta ggattacatt tgaatttcat
    24901 ggctacttta ttagatgaca acgtagaaaa acatgatact tggaaagaaa tacggacaga
    24961 aaatgctcac aaaatggcga ttaaggaatt gatagaacgc gacaagaacc atccgagtgt
    25021 cgtaatgtgg tctattgcaa atgaaccaga ttcggattct gaaggcgcta aagaatattt
    25081 tgaaccttta gttgaattga ctaaagaact tgaccctcag aagagacctg taacaatcgt
    25141 tacttactta aagtcaacac cagatatttg taaagtagga gatattgtcg atgttttatg
    25201 tttaaatcgc tattatggtt ggtatgtttc aggtggagac ctaaaacatg caaaatatct
    25261 gttgaaaaaa gagctagatg gatggatgga aagatgtccc gatacgccaa taatgtttac
    25321 agaatatgga gcagatacag tagctggact tcatgataca gtaccaacaa tgtttacaga
    25381 agagtatcaa gtgggttatt acgaagcaaa tcacgaagta atagataaat gtgacaattt
    25441 tgttggtgaa caagtgtgga attttgcaga ttttgaaaca agtcaaggga ttttaagagt
    25501 gcaaggaaat aagaaaggaa tctttactag agatagaaag cctaaaatga ttgctcacta
    25561 tctaaagaaa cgatggtcat caataccaaa tttcagttat aaaaaataat ttacctttta
    25621 taaggaatat aaaaaaggag tggctgctta attgccaact cctttttttt ataaaaagat
    25681 tttctgttta aagaaggata tttttttcta gttttttcga aaaaaaggcg ctttctgatt
    25741 gttgtattag tggaaagggg aaaaaaggag catatttatc atgcaacaaa attacaatga
    25801 gaaatctata caacatcaat ttgatgccta ctgtaaaaaa gtattacgaa acgaaactaa
    25861 gtctattcga aaacgaaaca ttaacataac agataatgag gaaccattga cgaattcaaa
    25921 tgaaggtaag tactcccaaa ctcattttga tgaacaagat gtctatttct tatttggaat
    25981 ggaaatatta atttcagatc aaaagttatc ggcagccatt gatcatttat cggaaataag
    26041 aagaaaggtt atcctacttt attattttgc aggttttaat gatactgaaa tcggtaaaat
    26101 cctcaatatg agtacaagtg gtatctggta tcaacgaaag aaggcagttg aacaattaaa
    26161 aatggggtat gacttatggt agacaacccc aaaaaacggc agtatccatt agttcctcta
    26221 tccattatcc aatctgctct agaagaagat ccaatttcaa tcaagatgat actcaaacat
    26281 tatcatagtt atatagctaa gttatgtttg acaaatggat ttaatgaggc ggggcaattt
    26341 attacctatg tagatgagta tatgctaaga cagctagaaa ttaaattgat agaagcgatc
    26401 ttaaaattta aaattaacta actaaaaagt cccctttcca cttattctgt ttaaaagaat
    26461 cttctaatag ttaaaggatt agtagattct tttaaacaga aaacctattg atcattgaaa
    26521 tttaaataca ttctatcagt acgttaacac ttcaaggagc atattttagt tgcgccatga
    26581 tttcttactg taaagaagga gcgataaaca ttctaaaata atttttatag tcaataaaaa
    26641 gcgatgattt gaagttggga taatgatact tccgtaaggc tcgccgtaaa cttggagaga
    26701 ggtgaaattc ctatgtgctt ttgacaaaag cagctgatta agaattattt ccatatgttg
    26761 atttcaacat tttttctatt atttttaaga ccaaattcag tgaggcaaat agcatcatgt
    26821 aaatttagtt tttctattga acgttctcca ctttttagtt tttttatagt actcaaattt
    26881 aatcctgtat gtttagagat ctcatttgca gtatggtttt gtaaagtttc tcggattatt
    26941 gttatattag caatttcatt acgcataatt atgttccttt cttatcaaaa atatcaatga
    27001 ctacagtggg cttttagtga aaaaaagggg tactacagtc tcttttgaaa acaaaaaaat
    27061 acagtatata cgttttctaa atgctaagca ccaacgttta cactgtattt aagggtttgg
    27121 cacccaatgg gctgtgaggg gctcgaaccc gcgacccgat gattaagagt catctgctct
    27181 accaactgag ctaacagccc aaattaatac tttgttattc taacacctaa aataaaaaag
    27241 tgcaatactt attatcagaa ttgagagtga attttatgga ttttcaagat tatgaaaaaa
    27301 tatttgaaag ttatccatcg atattgaaaa ttactgattt aactaaaatg ttaaatattg
    27361 gaaaaaataa agcttataat ttgctgaaag atagatcaat agaaaatata aaaattggag
    27421 caaggtacaa aatacctaaa aaagctgtca ttgagtatat attgaaaata caagatgaag
    27481 gtaaaaatca tgctataata aaatagtcaa aatttttgcc gatatttcag tttaagagga
    27541 gagattgaat tgaaattaag agcaaacata attgaaaaaa gaggaacatt acaagtagtt
    27601 gtatcttata aagactctaa cggaaaaatt agacaaaagt ggaaagatac aaaattaaaa
    27661 aagaaaggaa ataaaaaacg agcagaaaaa attagagatg aatttttact agaattggaa
    27721 agtcaattag ctatggaatg tgaatgtgct aatccaaatt tattgtttta tgactatttg
    27781 cttaattatt tggaagtatc aaaaaaacaa gtagcattta acacatatgt tgggtataag
    27841 cattacgtcc ataatcgaat ttacaagttt tttttatata aaaatagcat aaaaatctag
    27901 ttatccgcat aaaaactgga cttatcacac tttatcaagg tcaaaaccac tcaatttact
    27961 actaatttac tacttatgaa tgagctttga tacgacgatt tatccttgaa aagtgaagat
    28021 ataaagatac ttccaataaa atttgaatat ttaataggta gacacttcaa aaaatgaggt
    28081 gtctattttt ttacccgatt ttgaaaggaa gtgaacttat gaaaacaaaa aatcaagaat
    28141 caaaaggtcg ttccccactc tttaagacca tcaaacattc attcagccaa taaaaaagaa
    28201 aggataggta aaaatatgga acttaaattt gtgattccca acatggaaaa aacattcggc
    28261 aatttagaat ttgctggcga ggataaagtc gttcagcgaa gaatcaacgg acggctaact
    28321 gtcttatcaa gaagctataa tctctattct gatgttcaaa gagcagatga tattgtggtg
    28381 gtgcttcctg ctgaagctgg cgaaaaacat ttcggctttg aggaacgtgt gaagttagtc
    28441 aatccacgta ttaccgcaga gggctacaaa atcggcactc gtggttttac aaattacctt
    28501 ttacatgctg acgacatgat aaaagaataa agaaagagag gaaaaatgat gagattagca
    28561 aatggcattg tattagataa agacacgact tttggagaat tgaaattctc tgctctacgt
    28621 cgtgaagtga gaatccaaaa tgaagacggg tcggtttcag atgaaatcaa ggaacgtacc
    28681 tatgacttaa aatccaaagg acaaggacgc atgattcaag taagtattcc tgccagcgtg
    28741 cctttgaaag agtttgatta taacgcacgg gtggaactta tcaatcccat tgcggacacc
    28801 gttgctactg ccacctatca aggagcagat gttgactggt atatcaaggc agacgatatt
    28861 gtgctgacaa aggattctag ttcattcaaa gctcaaccac aagcaaagaa agaaccgaca
    28921 caagacaaat agtcgctagg tagaaaggag actttttcgc atgaaacagc gtggtaaaag
    28981 gattcgccca tctggtaaag atttagtctt tcattttacg atagcgtcac tcctgcctgt
    29041 tttcctgctg gttgtcggac tgtttcatgt gaagacaatc cagcagatca actggcagga
    29101 ttttaaccta tcacaagcag ataagattga cattccctat ttaattatca gtttcagtgt
    29161 cgcaattctt atctgcttgc tggtagcgtt tgtattcaaa cgggttcgct atgatacggt
    29221 taaacaactt taccaccgtc aaaaactggc aaagatgata cttgaaaaca agtggtatga
    29281 atctgaacag gtcaaaacag agggtttctt taaagatagt gctggtcgta caaaggaaaa
    29341 gataacctac ttccctaaaa tgtattatcg acttaaaaat ggcttgatac agatacgggt
    29401 ggaaatcacg ctgggaaaat atcaagacca actcttacac ttggaaaaga aattagagag
    29461 tggcttgtac tgtgagctga cggataaaga gttaaaggat tcctatgtgg aatatacttt
    29521 gctctatgac accatagcca gtcgtatttc tattgatgaa gtagaagcta aagatggtaa
    29581 acttcgctta atgaaaaacg tatggtggga atatgataag ctccctcata tgttgattgc
    29641 tggtggtaca ggtggcggta aaacttactt tatactgaca ctgattgaag ccttgcttca
    29701 tacagattca aaactgtata ttcttgaccc gaaaaatgct gatcttgcgg acttaggttc
    29761 tgtgatggca aatgtctact atagaaaaga agacttgctt tcttgcattg aaacattcta
    29821 tgaagaaatg atgaaacgta gtgaggaaat gaagcagatg aagaactata agactggcaa
    29881 aaattatgct tacttaggtc tcccggcaca cttcttaatc tttgatgaat acgtcgcttt
    29941 catggaaatg ctgggaacaa aagaaaacac cgcagttatg aataagctga aacagattgt
    30001 catgttaggt cgtcaagctg gcttctttct aatactggct tgtcaacgtc cagacgcaaa
    30061 atatttaggc gacggaatcc gtgatcagtt taatttcaga gtggctttag gtcgtatgtc
    30121 tgaaatgggc tatggcatga tgtttggcag tgacgtacaa aaggatttct tcttaaagcg
    30181 aatcaaaggt cgtggctatg ttgatgtagg aacaagtgtc atatcagagt tttatactcc
    30241 ccttgtacca aaaggatatg atttcttgga ggaaattaaa aagttatcca acagcagaca
    30301 gtccacgcag gcgacgtgcg aagcggaagt cgcaggtgtg gactgatctt gctggctggt
    30361 gtggcaatag ccacgccagc acttaacccc ccgtatctaa caggggggta caaatcgaca
    30421 ggaaacagtc aaaaaaacat tagaaaatcc tttggttaca agggatttac aaaatttcag
    30481 cgtatgtcaa atgggcttta aaagttgaca tacgcctttt tgattggagg gatttttact
    30541 gaatgaacaa acttggttac agcatttaaa agaaaaacgc ttggcttatg gactatctca
    30601 aaaccgttta gctgttgcga ctggtattac aaggcagtat ctaagcgata ttgaaacagg
    30661 aaaagtcaag ccatcagagg atttacagca gtccctttgg gaagctctgg aacgcttcaa
    30721 tcccgacgct ccccttgaaa tgctgtttga ttatgtaaga attcgctttc cgacaacaga
    30781 cgtacagcag gtggtcgaaa acatcttaca actgaaactg tcctattttc ttcatgagga
    30841 ctatggtttc tattcttatt cagagcatta tgctttaggc gacatattcg tcctttgctc
    30901 ccatgaactg gacaaaggag ttctggtgga attgaaaggt cgtgggtgca gacaatttga
    30961 aagctatctt ctggcacaac aaagaagctg gtatgagttc tttatggacg ttttggtggc
    31021 tggcggtgtg atgaaacgcc ttgaccttgc cattaacgat aagacaggga ttttaaatat
    31081 ccctgtactc actgaaaagt gccaacagga agaatgtatc tccgtcttcc gcagttttaa
    31141 aagctatcgc agtggcgaac tggtacgcaa agaggaaaag gaatgtatgg gaaacaccct
    31201 ctatatcggt tcattacaaa gtgaagttta tttctgtatc tatgaaaagg actacgagca
    31261 gtacaagaaa aatgatattc ccattgaaga cgcagaagta aaaaaccgtt ttgagattcg
    31321 attgaaaaat gagcgtgcct attatgcagt ccgtgattta ctcgtctatg acaatccaga
    31381 gcataccgcc tttaaaatta tcaatcggta tatccgtttt gtagataaag acgattccaa
    31441 acctcgttct gattggaaac tgaatgaaga atgggcttgg tttattggga acaatcgtga
    31501 acgattaaaa ctaaccacaa aaccagagcc ttactccttc caaaggacgc tgaactggct
    31561 atctcatcaa gttgccccga ccttaaaggt tgcgattaaa cttgatgaaa tcaaccagac
    31621 gcaggttgta aaagacattc tcgaccatgc gaaactgaca gaccgacaca agcagatttt
    31681 gaagcaacag tcagtaaaag aacaggacgt gataacaaca aaaaaataac tcaaatacaa
    31741 attcattgaa tatagagagg agaacatttt tatgaatttt ggacaaaacc t
//
LOCUS       DACDXE010000029        31700 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.029, whole genome shotgun sequence.
ACCESSION   DACDXE010000029 DACDXE010000000
VERSION     DACDXE010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.029"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(716..3865)
                     /locus_tag="IWM23_001352"
     CDS             complement(716..3865)
                     /locus_tag="IWM23_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9884002.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3878..5077)
                     /locus_tag="IWM23_001353"
     CDS             complement(3878..5077)
                     /locus_tag="IWM23_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9884003.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(5067..6662)
                     /locus_tag="IWM23_001354"
     CDS             complement(5067..6662)
                     /locus_tag="IWM23_001354"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9884004.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(7246..7953)
                     /locus_tag="IWM23_001355"
     CDS             complement(7246..7953)
                     /locus_tag="IWM23_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884005.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(8018..8854)
                     /locus_tag="IWM23_001356"
     CDS             complement(8018..8854)
                     /locus_tag="IWM23_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9884006.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(8872..10281)
                     /locus_tag="IWM23_001357"
     CDS             complement(8872..10281)
                     /locus_tag="IWM23_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9884007.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(10295..12118)
                     /locus_tag="IWM23_001358"
     CDS             complement(10295..12118)
                     /locus_tag="IWM23_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884008.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(12961..13287)
                     /locus_tag="IWM23_001359"
     CDS             complement(12961..13287)
                     /locus_tag="IWM23_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884009.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(13333..13695)
                     /locus_tag="IWM23_001360"
     CDS             complement(13333..13695)
                     /locus_tag="IWM23_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAP9884010.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(13732..13947)
                     /locus_tag="IWM23_001361"
     CDS             complement(13732..13947)
                     /locus_tag="IWM23_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884011.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            14074..14409
                     /locus_tag="IWM23_001362"
     CDS             14074..14409
                     /locus_tag="IWM23_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884012.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(14485..16482)
                     /locus_tag="IWM23_001363"
     CDS             complement(14485..16482)
                     /locus_tag="IWM23_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884013.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(16580..16855)
                     /locus_tag="IWM23_001364"
     CDS             complement(16580..16855)
                     /locus_tag="IWM23_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9884014.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            17087..17821
                     /locus_tag="IWM23_001365"
     CDS             17087..17821
                     /locus_tag="IWM23_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884015.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            17860..19002
                     /locus_tag="IWM23_001366"
     CDS             17860..19002
                     /locus_tag="IWM23_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884016.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(19097..20488)
                     /gene="sufB"
                     /locus_tag="IWM23_001367"
     CDS             complement(19097..20488)
                     /gene="sufB"
                     /locus_tag="IWM23_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAP9884017.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(20493..20963)
                     /locus_tag="IWM23_001368"
     CDS             complement(20493..20963)
                     /locus_tag="IWM23_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAP9884018.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(20950..22185)
                     /locus_tag="IWM23_001369"
     CDS             complement(20950..22185)
                     /locus_tag="IWM23_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9884019.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(22182..23468)
                     /gene="sufD"
                     /locus_tag="IWM23_001370"
     CDS             complement(22182..23468)
                     /gene="sufD"
                     /locus_tag="IWM23_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAP9884020.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(23481..24254)
                     /gene="sufC"
                     /locus_tag="IWM23_001371"
     CDS             complement(23481..24254)
                     /gene="sufC"
                     /locus_tag="IWM23_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAP9884021.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(24451..25299)
                     /locus_tag="IWM23_001372"
     CDS             complement(24451..25299)
                     /locus_tag="IWM23_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9884022.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(25323..26009)
                     /locus_tag="IWM23_001373"
     CDS             complement(25323..26009)
                     /locus_tag="IWM23_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884023.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(26002..27036)
                     /locus_tag="IWM23_001374"
     CDS             complement(26002..27036)
                     /locus_tag="IWM23_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9884024.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(27431..27769)
                     /locus_tag="IWM23_001375"
     CDS             complement(27431..27769)
                     /locus_tag="IWM23_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAP9884025.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(27771..28127)
                     /locus_tag="IWM23_001376"
     CDS             complement(27771..28127)
                     /locus_tag="IWM23_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAP9884026.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(28133..29320)
                     /locus_tag="IWM23_001377"
     CDS             complement(28133..29320)
                     /locus_tag="IWM23_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9884027.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(29511..30356)
                     /locus_tag="IWM23_001378"
     CDS             complement(29511..30356)
                     /locus_tag="IWM23_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9884028.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            30590..31555
                     /locus_tag="IWM23_001379"
     CDS             30590..31555
                     /locus_tag="IWM23_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAP9884029.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT         9183 a         6632 c         5332 g        10553 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttgggcact tgaatctcgg ctattttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cgtccttcac gaatgagctg attgggaaac tcttttgtta
     2041 accaatcagg ctctttcgtc atctctttga tggcccgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatt gcagatttcg ttggcctgcc atttcgaaaa tcaaattttg
     2161 tataggcatt atctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagc tgggtccaaa taactttctt ttttgacagg ttccatttga tcaatcatac
     2281 gattgatctg ttctgatgaa tactctgcat atgtttctac ttcttttaat ttccgataga
     2341 ttttattctc tagtgaagtc ggctccacgg tgttttcatg ctctacttga aatcctaaga
     2401 cagagccatc ttctagcgca ttcttaatgg tataggtatg caagacctct ccatactgat
     2461 catgggttgt acgtgccaat tgccctttgg cttgtttccg attctcagga aaaatcggtg
     2521 tacctgtaaa gccaaaccaa gtggatttcg gaaacatttt tttgatttct tccatatttt
     2581 cagcacttaa cgctcgatga cactcatcta ctacaaacac gatatgttgg cccattaatt
     2641 tttggaagcg attggttcct tttttctctt cttgtttctt tgcatatttc aacgccgcat
     2701 ctagtttttg acgggttgta atgattacaa catttgcatt ggcatctgcc aacagggttt
     2761 cacttaattc tttggcactt cccgttccaa cgatcaagct attcgctttg gcgttcccgg
     2821 aagaaatccc agtattaaat tccgaagcaa attttgtaaa ctctgtggtg gtttgattat
     2881 ctaaatcttt gcggtccact aacatgatcg ttcgatcgat ccccgatttt cgtgctaaaa
     2941 gcttagtaga tacaaaacta gtcaacgtct ttcctgaacc agtcgcatgc cacacatacc
     3001 ctgattgatg tttatttgcg gcaataaaca aggcttgaat cgcatgtact tgataaggat
     3061 gtaacaccat gagggtttta ttatcttgat cttcactgac gattgtataa tccgcaatca
     3121 aacgatgagc atccgggata tttaacactt gtttacaaaa ctcatataag ttttctactt
     3181 tttcattgtc tttcgttcgc caactaaata aaaacttagg gtgcaagtct ttcggtaagg
     3241 catttgcaaa ataccgagtg gtttgttcat tggaaaccac aaacaactgt aaggtcgaaa
     3301 agatattgtt ccgaaacata ccttcttctg catatttttt gatttggtta aatgcctgat
     3361 aaacaccatc ttttgcagtt acttgtttca gctcgatttg gacaatcggt aatccattga
     3421 ttaataaggt gacgtcaaaa cgtcgatccc gtgcttctat gttacttcca cgttttgcaa
     3481 tttgatggac cacttcataa gtagagattc ctccaccaat gtcttgattg gaataaagga
     3541 taagtgagac agaacccaat tgcgaatctt cccgttcaat cgtgatccga gccatcccat
     3601 tttcaccttt taaccatttc gcagcatcaa atggggtttt cgttcgcagc agtaattccg
     3661 ttttgatcgt ttcaaattct ttatcggtca aaggaaaatc attcagttct gcttgattat
     3721 ttgagataat tttttggcgt aaattgaccc aaaggtcttc ttctgatttt agatccggac
     3781 gatacgtcca ctgattatgc ccttctccaa gtacacgaat caactgttct tcgacttctg
     3841 cttcctcacg gtgactaatt ttcattcttc ttccctccta gacaaacatc ttttgtaaga
     3901 agcttttttt cgtttctttt agtaaatcta acttacgttg atgaagagcg atcgtatcat
     3961 ctagctgttt gaagaaagta ccgatttttt gttgttcatc ctttacagga accttaacac
     4021 tgatattctc aatatcagaa ctgttataag aaggcaatgc ccctacttgg gagtatcttg
     4081 gcaaatttat agtttcaaac aatattttac caaaatagat atcccaagta ttattaaata
     4141 aataagccat ggtattgtta tctattaaga aagggcttgt gaccaaacgt tttctattaa
     4201 gcattattgc tgcgccaact ttagcaaata taatcgctgg cacatctttt attggcttcc
     4261 attttttacg ctcaatttgc tcattagata cataatgatt tgcatttctc atttcatact
     4321 cattaccaat gttattcatg tcggaaactt taaaaaatgg agtaccttca gaaccacctt
     4381 gctcggcatc aggaaatcca attcccgatt gagttttacc taattctccc aacttacgct
     4441 cttcccaatc ttccgtaaaa ccaggaaacc ggatttctgg cacttttgct ccgttttcag
     4501 ggaacatttt ttgtaagaat ccttttttcg tttccttcag caaatctaac ttacgttgat
     4561 gaagagcgat agtgtcgtcg agttttttga agaatatgcc gattttttgt tgttctttta
     4621 aatcattggg aatatagaat actgtatctg aaactttttt ccagtctgaa cgaggcattt
     4681 ttgttccaga tgtatcattt gcagcctttt gatatctatc agcttgaata agagaataaa
     4741 taaagtccga gtcgctcttc ttagctctaa aaacccagaa atccccaatc gctacgcctg
     4801 taaaagttgc cttgagccaa ttttttaaat atggacgtaa ctttccatat agaatatcat
     4861 ttggtaaaaa ttcaatccct tttctattat caaatttctg gctcacatct ttattcagtc
     4921 taccttctcc tgccacgata tcttcaaatt caacttttgg aagtctactg gagtttgtac
     4981 ttttattaag tctaacaact aaatcaatta acttacgctc ttcccaatct tccgtaaatc
     5041 ctgggaaacg gatttctggt cgtgtatcat tgctcatgtt tgaacacctc cagcgcacct
     5101 tgtaaccaca tttcgttttc aggtgttgtt tgcagggaag agatagcttc taaaagttct
     5161 ttttctagca cttgctgttc ttgtcgtatc ttcttgatgt catttccgac atgaaccata
     5221 tcaattggtt cttcttcttc gaaagtatct acatagcgtg ggatatttaa gttatagtca
     5281 ttttctttga tttcatcaaa ggttgccaaa tgagcgtatt tctcgacatc ttttctttca
     5341 gcgtaggttt cgaggatttt ttgaatgttt tcttccgata gtttattttg atttttccct
     5401 ttaacaaatt cacgactagc atcaatgaat aatacatctc gagtttgacg attcttcttc
     5461 aacacaatca ccgtcgttgg aatcgatgtc ccaaagaaca agttcgcagg cataccgatc
     5521 acagcataaa tactgccatc ttctagtaat ttttgacgga tgaccccttc tgcagcgcca
     5581 cggaataaaa ctccgtgtgg taagacgatg gccatggtac ctgtttcctt caaatgatag
     5641 aacccatgaa gaagaaacgc aaaatctgct ttggattttg gcgctaattt cccataacga
     5701 ttaaaccgag aatcatctaa gaaagtcgta tctgctgacc aattagccga ataaggtggg
     5761 ttcattacta ctgcatcaaa tgtgtaaggt tcatctgttg gccagtcctt attcaacgta
     5821 tccccattgc ggagattcat ttcttcggcg tcgacaccat gtaaaatcaa gttcattttt
     5881 gctagattgt atgtcgttgt atttagttct tgcccatgat atttaacgtt atctgggtgt
     5941 gttaagtagt tacgtacgtt taacatcaat gaacctgaac ccatcgttgg atcaaaaaca
     6001 ctaaagaatc gtcgttcctt ttgatcgagc gttacgattt gcgccatcat atcagaaacc
     6061 atatgaggtg tgtaaaattc tccggctttc ttccctgctt ctgaagcaaa ttgactgatc
     6121 agaaactcat acgcatcccc gatcacgtcc ccatcgtgtt ctaaaacatc tacatcattc
     6181 aatttcttga tgacttcggt aatcgtcaca tttcgttgtt gctcatctgt tcctagtttt
     6241 tttgattgga gatccacatc atcaaataac ccattaaatt gattgtaggt agatgccaat
     6301 tgcacaaagg ctttgtttag atcgttcaat tggaacgttg cttgttttgc ttgatccgct
     6361 aagacgttga aaagatactc tggtgcaatt gcatatccca aaatatcaac gattgtggca
     6421 atcagatctt cttttgattc ttcatctgaa agtaattcac gatacaacat tgtttgtttg
     6481 cttactgtgt catattcctc taaggattca tctgctaaaa gaaccacttg ttccaacaat
     6541 cgatcggata aatatttata gaaaatcagt cccaataaat aatttttata ttctgacgca
     6601 tccattttac tacggagatt atctgccgca ctaaataatt tttgatttaa ttctgcaccc
     6661 aaaaagatac actcctatct cttatttaaa gttgctatat accctcttat tttagcatac
     6721 ccccttttat aaaaagcaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
     6781 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
     6841 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
     6901 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
     6961 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
     7021 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
     7081 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
     7141 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
     7201 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tctttttacc cattttcaag
     7261 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
     7321 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
     7381 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
     7441 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
     7501 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
     7561 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
     7621 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
     7681 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     7741 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     7801 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     7861 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     7921 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     7981 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     8041 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     8101 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     8161 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     8221 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     8281 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     8341 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     8401 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     8461 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     8521 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     8581 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     8641 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     8701 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     8761 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     8821 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     8881 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     8941 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     9001 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     9061 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     9121 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     9181 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     9241 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     9301 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     9361 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     9421 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     9481 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     9541 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     9601 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     9661 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     9721 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     9781 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     9841 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     9901 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     9961 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
    10021 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
    10081 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
    10141 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
    10201 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
    10261 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
    10321 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
    10381 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
    10441 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
    10501 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
    10561 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
    10621 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
    10681 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
    10741 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
    10801 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
    10861 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
    10921 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
    10981 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
    11041 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
    11101 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
    11161 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
    11221 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
    11281 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
    11341 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
    11401 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
    11461 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
    11521 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
    11581 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
    11641 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
    11701 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
    11761 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
    11821 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
    11881 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
    11941 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
    12001 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
    12061 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
    12121 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
    12181 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
    12241 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
    12301 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
    12361 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
    12421 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
    12481 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
    12541 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
    12601 gtaagcaata gccaatttca atcattccga gatataaagg tatcaataga atgatttggg
    12661 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
    12721 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
    12781 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
    12841 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
    12901 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
    12961 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
    13021 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
    13081 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
    13141 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
    13201 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
    13261 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgttc
    13321 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
    13381 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
    13441 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
    13501 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
    13561 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
    13621 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
    13681 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
    13741 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
    13801 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
    13861 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
    13921 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
    13981 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
    14041 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
    14101 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
    14161 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
    14221 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
    14281 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
    14341 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
    14401 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
    14461 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
    14521 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
    14581 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
    14641 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
    14701 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
    14761 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
    14821 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
    14881 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
    14941 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
    15001 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
    15061 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
    15121 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
    15181 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
    15241 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
    15301 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
    15361 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
    15421 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
    15481 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
    15541 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
    15601 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
    15661 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
    15721 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
    15781 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
    15841 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
    15901 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
    15961 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
    16021 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
    16081 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
    16141 aactaaattg atagataaaa ccttttctat gcttaagtat ctactatcat ctttactcaa
    16201 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
    16261 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
    16321 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
    16381 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
    16441 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
    16501 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
    16561 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
    16621 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
    16681 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    16741 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    16801 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    16861 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    16921 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    16981 gacatacaca taatacctcg ataaaaaagg ggtgtcaata gttttgtaaa atttatttac
    17041 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    17101 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    17161 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    17221 caaaaagact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    17281 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    17341 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    17401 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    17461 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    17521 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    17581 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    17641 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    17701 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    17761 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    17821 gtggtaagta ctccttgtaa actaagagga gatgatttca tggcaaaaat atcaaagtac
    17881 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    17941 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    18001 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    18061 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    18121 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    18181 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    18241 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    18301 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    18361 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    18421 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    18481 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    18541 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    18601 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    18661 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    18721 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    18781 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    18841 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    18901 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    18961 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    19021 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    19081 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    19141 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    19201 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    19261 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    19321 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    19381 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    19441 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    19501 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    19561 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    19621 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    19681 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    19741 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    19801 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    19861 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    19921 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    19981 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    20041 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    20101 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    20161 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    20221 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    20281 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    20341 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    20401 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    20461 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    20521 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    20581 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    20641 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    20701 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    20761 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    20821 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    20881 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    20941 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    21001 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    21061 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    21121 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    21181 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    21241 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    21301 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    21361 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    21421 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    21481 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    21541 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    21601 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    21661 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    21721 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    21781 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    21841 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    21901 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    21961 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    22021 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    22081 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    22141 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    22201 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    22261 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    22321 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    22381 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    22441 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    22501 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    22561 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    22621 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    22681 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    22741 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    22801 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    22861 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    22921 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    22981 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    23041 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    23101 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    23161 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    23221 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    23281 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    23341 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    23401 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    23461 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    23521 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    23581 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    23641 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    23701 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    23761 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    23821 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    23881 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    23941 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    24001 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    24061 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    24121 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    24181 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    24241 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    24301 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    24361 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    24421 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    24481 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    24541 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    24601 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    24661 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    24721 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    24781 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    24841 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    24901 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    24961 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    25021 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    25081 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    25141 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    25201 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    25261 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    25321 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    25381 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    25441 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    25501 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    25561 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    25621 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    25681 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    25741 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    25801 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    25861 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    25921 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    25981 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    26041 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    26101 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    26161 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    26221 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    26281 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    26341 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    26401 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    26461 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    26521 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    26581 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    26641 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    26701 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    26761 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    26821 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    26881 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    26941 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    27001 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttataa
    27061 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    27121 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    27181 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    27241 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    27301 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    27361 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    27421 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    27481 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    27541 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    27601 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    27661 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    27721 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    27781 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    27841 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    27901 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    27961 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    28021 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    28081 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    28141 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    28201 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    28261 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    28321 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    28381 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    28441 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    28501 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    28561 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    28621 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    28681 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    28741 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    28801 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    28861 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    28921 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    28981 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    29041 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    29101 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    29161 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    29221 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    29281 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    29341 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    29401 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    29461 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    29521 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    29581 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    29641 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    29701 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    29761 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    29821 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    29881 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    29941 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    30001 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    30061 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    30121 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    30181 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    30241 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    30301 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    30361 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    30421 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    30481 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    30541 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    30601 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    30661 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    30721 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    30781 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    30841 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    30901 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    30961 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    31021 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    31081 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    31141 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    31201 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    31261 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    31321 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    31381 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    31441 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    31501 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    31561 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    31621 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    31681 cgtttaccaa gaggtcgtaa
//
LOCUS       DACDXE010000030        30333 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.030, whole genome shotgun sequence.
ACCESSION   DACDXE010000030 DACDXE010000000
VERSION     DACDXE010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30333
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.030"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..597
                     /locus_tag="IWM23_001380"
     CDS             259..597
                     /locus_tag="IWM23_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884030.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(802..1041)
                     /locus_tag="IWM23_001381"
     CDS             complement(802..1041)
                     /locus_tag="IWM23_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAP9884031.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(1017..1364)
                     /locus_tag="IWM23_001382"
     CDS             complement(1017..1364)
                     /locus_tag="IWM23_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAP9884032.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(2096..2677)
                     /locus_tag="IWM23_001383"
     CDS             complement(2096..2677)
                     /locus_tag="IWM23_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9884033.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2860..3486)
                     /locus_tag="IWM23_001384"
     CDS             complement(2860..3486)
                     /locus_tag="IWM23_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9884034.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3508..4839)
                     /locus_tag="IWM23_001385"
     CDS             complement(3508..4839)
                     /locus_tag="IWM23_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAP9884035.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4994..5395)
                     /locus_tag="IWM23_001386"
     CDS             complement(4994..5395)
                     /locus_tag="IWM23_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAP9884036.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5481..5687)
                     /locus_tag="IWM23_001387"
     CDS             complement(5481..5687)
                     /locus_tag="IWM23_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884037.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5782..7284)
                     /locus_tag="IWM23_001388"
     CDS             complement(5782..7284)
                     /locus_tag="IWM23_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAP9884038.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWM23_001389"
     CDS             complement(7302..7757)
                     /gene="argR"
                     /locus_tag="IWM23_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAP9884039.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7855..8472)
                     /locus_tag="IWM23_001390"
     CDS             complement(7855..8472)
                     /locus_tag="IWM23_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884040.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8693..10066
                     /locus_tag="IWM23_001391"
     CDS             8693..10066
                     /locus_tag="IWM23_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9884041.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10141..11253
                     /locus_tag="IWM23_001392"
     CDS             10141..11253
                     /locus_tag="IWM23_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAP9884042.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(11325..12962)
                     /locus_tag="IWM23_001393"
     CDS             complement(11325..12962)
                     /locus_tag="IWM23_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAP9884043.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13130..13738)
                     /locus_tag="IWM23_001394"
     CDS             complement(13130..13738)
                     /locus_tag="IWM23_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9884044.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWM23_001395"
     CDS             complement(14122..15273)
                     /gene="queG"
                     /locus_tag="IWM23_001395"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAP9884045.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(15292..16044)
                     /locus_tag="IWM23_001396"
     CDS             complement(15292..16044)
                     /locus_tag="IWM23_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884046.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(16185..17135)
                     /locus_tag="IWM23_001397"
     CDS             complement(16185..17135)
                     /locus_tag="IWM23_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAP9884047.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(17136..17975)
                     /locus_tag="IWM23_001398"
     CDS             complement(17136..17975)
                     /locus_tag="IWM23_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAP9884048.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(17972..18718)
                     /locus_tag="IWM23_001399"
     CDS             complement(17972..18718)
                     /locus_tag="IWM23_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAP9884049.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            18956..20326
                     /locus_tag="IWM23_001400"
     CDS             18956..20326
                     /locus_tag="IWM23_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9884050.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(20381..21277)
                     /locus_tag="IWM23_001401"
     CDS             complement(20381..21277)
                     /locus_tag="IWM23_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9884051.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21558..22235)
                     /locus_tag="IWM23_001402"
     CDS             complement(21558..22235)
                     /locus_tag="IWM23_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9884052.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(22326..23231)
                     /locus_tag="IWM23_001403"
     CDS             complement(22326..23231)
                     /locus_tag="IWM23_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAP9884053.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            23406..24830
                     /locus_tag="IWM23_001404"
     CDS             23406..24830
                     /locus_tag="IWM23_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9884054.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            24876..25907
                     /locus_tag="IWM23_001405"
     CDS             24876..25907
                     /locus_tag="IWM23_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9884055.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            25914..27101
                     /locus_tag="IWM23_001406"
     CDS             25914..27101
                     /locus_tag="IWM23_001406"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAP9884056.1"
                     /translation="MEIKRSLNTKFAEIFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            27257..28639
                     /locus_tag="IWM23_001407"
     CDS             27257..28639
                     /locus_tag="IWM23_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAP9884057.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            28611..28997
                     /locus_tag="IWM23_001408"
     CDS             28611..28997
                     /locus_tag="IWM23_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884058.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(29197..29865)
                     /locus_tag="IWM23_001409"
     CDS             complement(29197..29865)
                     /locus_tag="IWM23_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAP9884059.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(30054..>30333)
                     /locus_tag="IWM23_001410"
     CDS             complement(30054..>30333)
                     /locus_tag="IWM23_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9884060.1"
                     /translation="WAYQMKAYRNKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEI
                     FHGKVYNCFEELKSAIDSYIYYYNNERIKQKLNWQSLVQFRKTTVTVV"
BASE COUNT         8516 a         6199 c         5522 g        10096 t
ORIGIN      
        1 tatataacac taagtaacgc aaaattcccc tttgtaaact atctagaaaa ttgttttgtt
       61 taaagtatat cttattggat ataaatagaa ataaattgta gccagttcat tttttcttta
      121 actaaaaaaa taatccaaga aacattgaac ctatggatcc aaaagctacc caaatactat
      181 agctaacccc ccgctacctt caattttgca gataaaacgc tttgggagaa aatttcaaag
      241 acaacaggcc atcctatcat gcccgattat aaacaaaaag ctggctattc cttttgctct
      301 gacaaatttc aattgggaaa gttcacgaaa ctagcaggtg aagaagtttc aactgttcgg
      361 atcaaagaat gcccgcttca aatagaaaca ttggttcaac gaatttcaac tagacaagac
      421 tttgctattg tagaacgtga gatcgtagct atttttgtag aagaagggat tttacatgat
      481 ccaactcata tcaatgtgga taaatggcaa cctcttatct ataaattcag agaatataca
      541 actgccaacc agtcgttaac tttgaatttt agattccaag aattcaaaga gtcataatga
      601 taaacatcgt taggtataaa taaaaaagac tcgcagcacg agtaaaaaac aaatagaagc
      661 tatacattag aaaggtcgac tgtgacccta tagatgcagt atatgcagct tatgagcatt
      721 tcttctgttc tctttgtttt tgggatctgt gctgaagtct ttttcgtttt ttgccgctct
      781 ttctttgaaa aatcgattat gttatacctt ctccattact aattcaggca tctgctgagt
      841 aatgattttt cgcgcaaaag aaatatctgt tacccaaggt tcgatttgtt cttcagtcaa
      901 gacaagcggc attcggtcat ggattggtgc gactgtgcgg ttaggttcag tagtcattat
      961 gattgattct gccatttttt catcctgatg gctacgataa aaaccgccaa tatacactat
     1021 tttttcctta tttgaaaaca taaatttgtc tttttcttca ttccactcat tgggaaaaca
     1081 caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt cttctactgt ctctgcacgg
     1141 gcattaatca tcaattgtcc ttttttaaat ccggtaaatc cccacttcat gatttccgga
     1201 gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa gatttgacgg aaaaacttct
     1261 ccagctgcta attctgctgc ctgcttcggt tgtttcgtct gggcttctcg ataataattc
     1321 tgaaggagct ctgactctag atcaaaaaaa taacgaccac acatcctaat ccctcccaat
     1381 ctttttttct atcatacact gcaacaagaa gttcgcacca acaaaacgct ttcaattttt
     1441 gtagaactaa aaaagttgtg acaagtgaca aactctccag tgaatggtat tctcctgcca
     1501 actcctcggt cacaagtcac aatattcaac aaacatgaga agctgctttt tgaaattgtt
     1561 ccttgtgact cggagtgaaa cggcaggctt acgacctctg ggtaaacggt gttcaaaagc
     1621 cagtttctcg gtattaagcc aaatttatgc aaaaatgtag aaagctattt tcacaaaatt
     1681 tttcttaata ctcgaaacta agcgactttt tcacaacctc ttccttaaac ggtgttcaaa
     1741 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagcagct atttttattt
     1801 ggctgttctt attacttgat gcagggcagc tttttcacaa cctctccact aaacggtgtt
     1861 caatcagcta accttcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttgcc
     1921 gtttgtttct ctaatactca ggtcaaagcg ctgatttcac aacctctaat acacggtgtt
     1981 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaatttgag aagcaatttt
     2041 cgtaaattcc ttcttatttc ttgaagctga ccacttctgt cacaacctct aattattaaa
     2101 gaaccttttg cgaagttaac aatctttctt tgatcaattg aaaattctgc ctaagtagtg
     2161 atagatcctg tgtgactttc aacaataaga tacttccctg gatttcagca atcaacgctt
     2221 tggcttgacg gtctgcttct tcatcggaca gtttttcttc agaaagtgcc aggatatttt
     2281 gtttcgtata cttgacccat tgattgacta attctagtag caacagacgg tattcttcat
     2341 ccactgtact caactcaata atcaaattac ctacaggaca gccatgataa gtgatctgtg
     2401 tctcatgatc agcgaaaatc cagtccaaca tttcttctaa tgcttcttct ggctgttttc
     2461 cagcatttaa gatgtcttcg aacaattctg tctgccatac gtccatatgc gcatggatca
     2521 ccgctagaca aacttctttt tttgaagaaa agtaataata aaattgacct tttcctgtat
     2581 cagaagctgc tagaatatct cggattgacg tttcatgata gcctttttta taaataagat
     2641 ctttggtcgc ttccaagatc ttttcctttg ttttcaatga tattctcctt tttcatttct
     2701 catagtccta tattatacag taaattcatc attttttctt cttaatcgtt taaaaaagac
     2761 tgtgccaaat tatctgacac agccctcact ataaaactgt tctcatatag ctagatgttc
     2821 attgattcaa gaactgcgag gacaaaagca attcctatct tatgatagtt gctctaatac
     2881 ttcctctgtt gtcatcactt gatctgcaat aaagccaaag ttcgtcaacg ctgcatccat
     2941 tgcttcttgg ccaggagcac caattgcatc tgtcgctacc acaacattga atccactctc
     3001 taccaattca cgcatatggg aatcgctaca caagtttccg ttcattccac caagaattac
     3061 tgtgtcgatt ccattttttc tcaattggag cgccaaatca ttgctttctg gtccaaagat
     3121 tttatgagga gagctgatga tcgtattttc atccaaataa ggtttcaatt cttcgataaa
     3181 atcggctcct gaaccttctt ccactgattc atattgactt tttctagcaa acatctgctg
     3241 ctcaagcatc atatgttctc ctgcaccgcc aaattgccat ttatggtcat gtgggtaaaa
     3301 ataatgagga gaaataaaga gtttgtatcc tttttctttt gcttgtttta ccatttcaat
     3361 catattttcg atcgtattgt atttttctaa gatatctttt gtcaaaccaa agccaccgcc
     3421 tgctggtttc aagaactcga cttgcgggtc agtgagaact actgctgtat ttttattatt
     3481 gaacatggtg gattctccat tcgttattta ttttaataca tcatttagag cttcactcat
     3541 tgtcggatga gaatagattt gatctctcaa taacgtataa ggaagttttc cattcatagc
     3601 tagcgaaatc aagttgatga cttcatgaga atcttccgca tagatacttg cccctagaat
     3661 caagtcagtt tcagggtcga ctaaaacttt gaatacacct ttaggatctt ctaatacttg
     3721 tgctttaggg acaccgcttg tcatatactt gaataatttg taattgacgc cttgtgcttt
     3781 ggcttgtttt tctgttaaac ctacattgga taaagtcgga gtgataaata cactgtaagg
     3841 cacagctttc cggttattcg ttgttcgttt attctcgccc ttcaactgat ccataatgat
     3901 tcgataatca tctagagaga tataagtgaa ctgcaatccg cctttgacgt ctccaatcgc
     3961 ccaaatgttg tctgcatctg tacgaaggta ttcatccaca gcgattgctc cgcgatctgt
     4021 tagtttaaca tctgtatttt ctaatccaag tgatgctgta cttggttttc ttccagttgc
     4081 agccaaaact tttgacgctt ggattgttac ttcttggcca tcttctgtaa aggtgacggc
     4141 agctgttgat tcttggtctg ctacttgatc tacagataca cctagatgga attcgattcc
     4201 cgcatttgtc atgtcttcta aaatcatttg cgcgatatct tcatcttcac gtggaagaaa
     4261 ttcagaatgg gcatcaagaa cgacaacttt agatccgtat tcattgaaca ttgatgcaaa
     4321 ttccaagcca atatatccag ccccgatgat cactaatttt tccggtaatt gttcctgatc
     4381 catcgcttgc gtagaatcaa tcaagtagcg acttgtattc aatcctttga ttggtaaaat
     4441 gattggttca gaaccagtat tgatgaaaat ccgttcgcct tcaacaatca ctttttcacc
     4501 attattcgtc acttcgattt gatggttaga aagaaaacgt gctgtaccgt ccatcactgt
     4561 cccagttgcc tcatcgctga tcatatgata atttttggcg cgaagcatgc ccgtcaattt
     4621 ttctttccga gcaacagcct ctgtaaagtc gattcctctt tctccattga aaatcaatga
     4681 tttagaagga atacagccga tgttgataca tgttccgcca tacatacgtg ttgacttttc
     4741 aacgactaaa accgattcac cttttgaagc taacgccttc gctaaagtct taccgccctt
     4801 tccaaaaccg atcacaatat tttgatattt ttccatcatt tttccctcgc ttctttttta
     4861 cctaccgatt ggtacaaaaa tatgataacc gataattgtc tattctccaa atcatctgct
     4921 ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca cgtttttagt ctggttcgat
     4981 ttcctttatt cttctaaaca atcgttactt ctacatcgat atttcctcta gttgctttgg
     5041 agtaagggca gaattcatgc gcatcttctg caattttttg ggcttcttct tcgctgatgc
     5101 catcgatatg cacttctaat actacaccga tctgatagcc gccatcatct aaattataaa
     5161 gagagacacg tgcttttact gtggactctg ctgtcattcc tgcttctttg atcatatgtt
     5221 gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa taattgttct ggatttgttc
     5281 cttctgcatg gattcctgga ggagtgattt tcatatgcat cgagccattt ggtgcctcga
     5341 cttctccatt gcgcccgcca tggttgatca ttgctgtttc gtatgctttt ttcattgtac
     5401 tgctcctttc gattgaccat ttgctacttg gctataatta tagtgtatga caagtaaggc
     5461 gctttctaca aaaattctgc ttattcgaat accagaatgc cgtcttctac tatttttcca
     5521 aaaaacaaaa aaatactact cgtgaacaac agcgtcatat ttgttccaaa ataaatagca
     5581 atcttttcta cataatcttt tatatgattg attttaagat acatatatac cagaaagttg
     5641 accccataaa ccaatactgt taaaaaaata accatattcg ttaccatttt ttcctccttt
     5701 tgctggtctc cctaccagca ctcgatttaa ccataccact ttttttgccg tccaagcaac
     5761 tagcctttca aaaagactgg tttattctgc tgttaatgat tcaagatcag aaaaatgtcc
     5821 gaataatttt gctaatttta ctaacgccaa cttgttcttt ccgaattcat cattttctat
     5881 ctgtaatact agtaacggct catgtaagct taaacggatc aaaaaccagc cgtttccgta
     5941 tggctcttct agatcatatc gaataccttc ttcattttcc gggtcgaagt tcatcccttt
     6001 ttcttcttcc aacagtcgct tcatttcttc taatatcttg tttccttgtt cgcgtggttc
     6061 atcgctgaac aattttaatc ggatttcttg tacttctacg ggctgtttca actctttgat
     6121 ttgttcatct aatgtgatac cttttctttt taattctggc aaaagcatca ggatctttgc
     6181 gatgacatat gcaccatcat ccagaaaata gttttccttg aatgcagcgt gtccgcttgt
     6241 ttcgatggct agctgtgtat ctttcccaag acggttaagt tctaatgctt tattgattac
     6301 atttcgatag ccggaaatat aacgaacttg gatgcctcct ttttcttcaa tgaatctttg
     6361 tagatgagaa gaagtagggg aattcgtcac gattcttgtt cctggatgct cttttaatac
     6421 gattgtactt aatactgcga tcaaattatt tcgattgatc actgttccag aagcactgac
     6481 aactgcagaa cgatccacat ctgtatcgaa aatgatccca agatctgctt catttgctaa
     6541 aacagcctgc ttgatacttt ccatcgcttc tttattatca ggatttggga tatgattagg
     6601 aaatcggcca tctggttcca aaaattggga acctgttgaa tccgcaccta agctattcaa
     6661 tactttttca gcaaagaatc caccagcacc atttccggca tcaacaatga ttttccaacc
     6721 atttagtggt ttgctttcta gtgttccaat ccccttttta atttttgtga ctaaatcttg
     6781 cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca gtttgttcgc tttttatttt
     6841 ttgctgctgt gataggataa aagcaatatc ttctttttct gctcctcctt ctgaggtaaa
     6901 gaattttatt ccattataat aatagggaag atggctagca gtcagcataa ccgcagcatc
     6961 gcacgagaac tgttcaaatt gagtggacat aaacatagca ggcgtagtag ctaattcgaa
     7021 atcgatgaca tgtatccctt gttctttcag actttggatc aacgcctctt tcagtgctgg
     7081 tccgcttatc ctactgtcat gaccaatccc aatcgtccat tttttatttg gatcggttga
     7141 tttcttcttt ttctttatcc agtttgctag tccccagccg atctttttta ccatgtctgt
     7201 cgtcaagttt acttcatatc cttcaactgg taatgcgatt cctcgaatat ccgatccatt
     7261 ttgcagcgct ttcatatctt tcatataaaa gtcctcctca tttattccct cattttttcc
     7321 agcttttctg caaatcgctg tgcttcttct tctgagcggc aaataataat gaatgtatct
     7381 acaccggcca tcgttccgac aacttcaggg taagccactt catctagcca attcgttacc
     7441 acatcggcac catttcgttc agtcaatacg ataaccatga actgaacaga ctggatacgc
     7501 aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat ttaaagaaac agatggctgg
     7561 ttaaataatg tatagcgtac tattttgttt tcattttgac tttttactaa cttcatttct
     7621 cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc ctgcttcatg cagccgcgca
     7681 agcagctctt cctgtgtccc aataggttgt tctgtaatga tctggcggat cagtgcttgt
     7741 cgctgtttct ttttcatatt agacacctct caatgttgat tatagcaaaa gataagtaat
     7801 atttcttcat attttggtat tttttacttt tattttttgt tttcattcag gttgctaacc
     7861 tttttctatt ttaacacgca aaatcgtctt cattttttca ggtgctgatt ttcttggatc
     7921 gctgatgtaa atctctttgt gttctttaga agctctttca tagccttctt ttgcgatgaa
     7981 tgcttctaat ttctcaaacg ttgctggttc ttcggcatag ggcccgacgt gtaagatttg
     8041 ggcacaaaga ccttcttcca tctttttgaa tactacttgt tctttcaagt cttccggaag
     8101 tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct gtaacaaagt caggctgtcg
     8161 gatcatcaat tgataagaaa aatgttcttt tttcatcact ggttcatgca aatacttttc
     8221 ttgcagtccc cattgtcctt ccagtggata gacagtatac atctgataat caggaatcag
     8281 ccaatttttt ttaggcgaca tgcgtattgc atagctgact gcatatagac aggctactct
     8341 tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg atgctaaaga atgatttttt
     8401 aggaacctct agtatttgcg ggcttttttt catactatag ctcttgtctt tacgccattc
     8461 aattttttcc atttttcttc ctccgcgaaa ttccatttag ttatttatta tcatacctct
     8521 ttttgagtga cttggctaat cgtgttgaca ctatttttca gtttgtgcta gaataagtga
     8581 agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga gagagaggcg gtttctattt
     8641 ttataataga aatcactctc tttttttacg aaatttttta gaggtgatgt aaatgacaaa
     8701 acaaaaacag ctgcttttag tacctgcagt aattgcaaca gggatgatgt cttttgcggg
     8761 cgtattgatc gagacggcca tgaatgtaac tttcccaact cttatcagac agtttgattt
     8821 aacgactgca caagtccaat gggtaacaac gatttatctt ttgatgattt cgatcattgt
     8881 tccgctgtct acttatttga atcgcaattt tagtctcaaa aaactctttt tagcttcaaa
     8941 tctgcttttc ttggcaggta taatgatcga tttcttctct cctaccttca tggtcctatt
     9001 gtttggtcga ttgctccaag gtgcagcgac aggtatcgct cttccgttga tgttccatat
     9061 tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg atgggtgtcg gtactttgac
     9121 aacggccatt gctccagcaa ttggccccac ttatggaggc attatgactt cacattttac
     9181 atggaatcat atcttcctgt ttcttgtgcc cgttttgatt ctttctttga ttatcggctg
     9241 gtttgctatt cccgagatgg cagtacaaaa aagtggttat ttggattgga taagcgtttt
     9301 aggaatttgt cttttgttta gtggaagtct aacatttttg agtacgttgg gaacagtttc
     9361 cagctggatt gctttaatca tcggcggtat tggctggttc cttttttact atcgaagtaa
     9421 aaaagtggag catccactga tccgactgtc tattctttca aataaaacat tccgcttgtt
     9481 tttgattggc ttcttagttt atcagttttt attacttggt gtatcttttg tcttgccgaa
     9541 tttccttcag atcgttcaag gaagtagtcc ttttgtcgct ggattagcga tgcttcccgg
     9601 cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa atgctggatc aatatggtcc
     9661 gaagaagcca atttttgctg ggctgattct ttctttgatt ggttggctag cattattcct
     9721 actcatagga cacgcttcag tgggtgtatt gattgcttgt catgtgtttt atatgatcgg
     9781 tatcggatta tcttacagca atatgatgac caccggaatg aatgcactga atgaagaact
     9841 acaaggagac gggaacgcaa tgttcaatac cttgcagcag ttttctggcg ctgtggctac
     9901 ttcattagta gcggtcatta tcaattacgt ccagcatcat acttcccata actatgaagt
     9961 gtctactaca ttaggatcaa aagcagcatt aggcgtcttg ttgctgttgc ttctcgtaag
    10021 ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa gcgtagattc catatacggc
    10081 aggtgttgca tctgccgttt ttttgtgtga aaatggttgt ttgtaaaagg aggctttttt
    10141 atggaaaaaa ttgccattat cggtggcggg atcattggta tgaccctagc cagttactta
    10201 gatacatcaa aattcaatgt tgctttattt gatgaaggga caggacaggc aactaaagct
    10261 agcgctggga ttatctcccc ttggttgtcg aaacgcagaa acaaacaatg gtatcgtctg
    10321 gctaaagacg gcgctgcttt tttccctaaa cttataaaag atttccaact tgatcaaacg
    10381 atttacgcac aatcagggac acttttgctt cgacctgaaa aagatttagc tgaattggct
    10441 gttcttgccg agaaacgaaa agaagaagcg ccagaaattg gaaacatcga atggctttca
    10501 cctgaacaga catctcaagc acttccgtta ttgaaaccag cagtttcact gcgtatcagt
    10561 ggtggtggtc gtctggatgg aaaagcatat ttagaacatc ttggtcagcg tttagcacaa
    10621 aaacaaatgg agcagatcca ggcaaaagtc actttgcata agaagagcga ttcttggctc
    10681 atcaagggac cgtttggaga atacacagct gatcgtgtaa ttttgacacc tggcccggca
    10741 cttaagcctt tattacaaga attaggctat gctgcagata tacgtccgca aaaagggcag
    10801 ctcttagcat ttcagacatc ttataagatg agtcaagact ggcctgttgc tatgcttgac
    10861 ggagaagctg atttcattcc tttcatggat ggaaagatcc ttttaggtgc tacccatgaa
    10921 aacgatcaag gctgggattt ggctcctact agcgaagctt ataagggatt gactaagagt
    10981 acagaacagt tcttgactga tccagatcag ataaaaaact ggccatacga gttccgtgta
    11041 ggtacccgtg cttatacgtc agatttttcc ccatttttcg gcgtactgcc agatgatcca
    11101 agcttactgg ctgctagcgg tctaggctct tctggcttga ccacaggtcc ttatatcggc
    11161 tatctattag ccaacttctt aaatactggt cattggccta cggaagatta tcaaaaggac
    11221 atctccacct atattacgaa aaatccatca tagaaaaaca gctttttcaa gtaaggatcc
    11281 cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt tattttagtt caatttctga
    11341 atcaaattct ccaaactttc ttttgcttca tctggcaaga gatcttttcc tatctgagct
    11401 tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca gatttttata ggacgtatcc
    11461 tcaaaagaaa caggaaaatc tggtataggt aaatagtgga tgtcttcaaa tggaccaaac
    11521 gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga ttgacaatgt cgtttcaggt
    11581 ttgatttttt tatcatcgaa atatccagta ttcaggacat agcaggctgt ttgctgtttc
    11641 aaaaacaact ctcgaaaagc ttgatagtct tccctaagag gatagacacg gaaaggatta
    11701 gcaaatggtt caatgactag ctcatctagc tgtctttttg tagtgatatt ttccgcgtct
    11761 gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta aaactgggtc attgatcttg
    11821 ataattggcg gaagcgaatc atctttcatg atccaaaaaa cagcatcgat cttttcgcta
    11881 agatgatcga ctcggtcggg tgtcgcataa cgtgatttga ctgtccgccc atttccgttt
    11941 cgtatatctc ctgttactag gactttttct cctttacttg tacatgtcac gcccacattc
    12001 tgacaagtaa ggaaagaaga aatggccgga tcatcagacg gatagtcttg tgttttatca
    12061 aagtaagaag gttccaaggc agtagcaaaa ccattttttc gagaaataac gaaagcatcg
    12121 tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg ctaaggtaat cgttgatttt
    12181 ccagaaccgg acaacccaaa tacagccatc gtataggtct ggtcattact agcatattgt
    12241 ttcatcccac cgtggcaggc aataaagcca ttgcgatttc ccaatgccca agcaagcgtc
    12301 aaagtcgctt tttttaattc tccaaagtaa cgtaatccta gcacagccgc tgtattatgg
    12361 agagcatcaa acaaaatgag tccatttgga aatgtttcat gtgtccattc gggatcacaa
    12421 aagatataaa gatctccctc atcaaaggaa acagactttt gataagtctc taagatcttc
    12481 gaattcgctg gctgaaaatt cagcaaataa gaatacaaat tgttctcaaa tccttctgga
    12541 atcagcaaat gactttgcac agaaaattct tcagataggc caacgattac ttttgtatgg
    12601 taaaactggc gcttgcgact ttgatacaca gcttctctta aaattttctc gtatttgctt
    12661 tgttccttgt ctaagtcctc aaagatccgc cgagcacttg ctgttcttcc cacaatgctt
    12721 ccatcattga atactagtgt cctaacatca gctggtaaat ctagttcttc agcatgtgag
    12781 attgttaaat ctgtgatgat cgttcctgga ctcccttttg ctagttgata agctgtcttc
    12841 ctatcaaaaa cttcatgaac attattaccg taaaatgtcg tttctacaat cgatcgaata
    12901 ctggataaca gtggatttcc acttgtgatt tcgtttaatt cgagactctc ttttgtactc
    12961 atggctcctc ctcattccct aaacaaaaaa acgttttctt tattatacat tattcgagtt
    13021 tttatttgta ctgctgcgct gtgggaagca aacgagttat ttttcacaca aaaaaaccga
    13081 gaacttgaat aaacaagttt ctcggtttca tcattttcct tttatcggct tagtaccagc
    13141 cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc ataacgagat gttacatatt
    13201 gttctgctac acgttcttga ttggcagctg aatagtcgcc gtttaagtat gaagcatcta
    13261 attgataacg gccgatgtaa cgtccgttag tagctgtgta tgaaccgcct gattcttttt
    13321 gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt tgcagcagct ggggcagcag
    13381 gagctacttc aactgtttca acaggtgcaa cgtatgtatg ttcttgcgtt gctgtttcag
    13441 ttgtagatgc tgtattttct gttgttttgt tttctgttgt tgctgaagaa tcatttgaag
    13501 ttgggattgt taattgttca ccaacataaa tgcggttgat gtttgcgatc gaattttttt
    13561 cagcaattgc atcgatcatt gagttgtctc ctgcaaattt ttgtgagatc ttagataatg
    13621 aatcacctga ttttactgta taaacttcat cagcgtgagc atttgtagtt cctacaaaca
    13681 ttgcagtagc accagcagca aaagttgtac ctaaaacaat tttcttaaat gaattcatat
    13741 taaactcctt tttttctccg ttttttattc tttcgacgct attaacttta tcatttagaa
    13801 atatttcata ggtaaaagaa acatgacatt taatgacaaa ataacgaatt atttaatatt
    13861 tgtaacattt aaacaaatat ttttttgata caaaaccata aaagccttgt tgtataagca
    13921 aagtaatcgt ttttctatgt ttttttattt ttgaatgaaa aacgcagatt ctgtaacatt
    13981 cactgcaata atccataaaa aaaagcaaaa aaatttttga tttttatcaa attctcttaa
    14041 aaaaatgtaa aaaatacaaa aaaaccagta attgttacaa caattactgg acaaaaactt
    14101 tgaataatgt taactatttt tttattttaa ctttctccgc aataatttct gggcatgttc
    14161 caattcgttt ataacgactt cttctgtttc tttagctaat atttcatcta atacatcaag
    14221 ccatttttcc ggttcttttg ttcctagacg tccaatagcc caagctgctg ttcctctgat
    14281 gacagggcgg acatctgtta aacattctga aatctcaggc aatgccgttc gatcacctag
    14341 attagctaaa gcaatcaatg cgtttctctg caaaggtttc ttcccacgcc atgaaccagc
    14401 taaatgccca aattgctgtt tgaagtcttt attagacatc gaaagcattg gctttaattt
    14461 tggatatatt tcgtctactt ttggttccat ctcttcatga aaatgaaaat cttttccttg
    14521 attgtaaggg cagactaact ggcagatatc acatccataa atgacatttc gcattttttt
    14581 acgatattct tccggcatca tcccttttgt ctgagtttga taagacaaac atcgctgggc
    14641 attcattctt ccgtcaccaa gcaatgcttt tgtcggacaa gcagtcacgc agcgcataca
    14701 gtcaccgcaa ccaaattctc ctggtttatc aggctcaaat acaatattag taacgatctc
    14761 accaagatag acaaatgaac cgaactcttc agtgatcaac aatccgtttc gtccaataaa
    14821 tcctaaacca gctcgctgtg ctacagcgac atcgatcagt tctccagtat ctacttgcgg
    14881 agctagacgc cattcctctt ctttttgcca tttttctgct cttgtttgga tgaattcgat
    14941 caatcggttc aaacggtctc tgagtatatc atggtaatcg atcccccaag aagcacgagc
    15001 aaactgcccg cgtttttcat ccctaggcac ttcttcatgg atcctagtag gatacgcaag
    15061 tgcaatggaa atgatcgatt tcggctgatc aaatgtcatt tctggataaa gacgctcatc
    15121 gatatttgga tgttcaaagc cagaagtatg gccagctgct ttttgttctt ccaacgattc
    15181 ttttaatgaa tcaaaaggtt ccgccgtggt gaacccgatt ttgtcaattc ctaatcgttt
    15241 gctttcttgt atgatttctt cttttaatgt catggtcttc ctcgcttgct gttaacggct
    15301 gtataaccca taatatagtt tttctttttt cagctggaca aactggatct gctgatcggc
    15361 aatcttcttt tcttccatcc atttttttct tgcagcaaat acttcccaat aatttgttga
    15421 aattaatttt cttttgttat ctaatgcatt aaaaaaattc aatgaaactg gttttgtact
    15481 taacagttca tggagcctta cctcttccca tggcgaaaac tcagacagat cgcgggaata
    15541 caatacttct tcatagagat ttttttgctg tgtgtacgtg aatgtttgcg ggattttccc
    15601 aggcctagca atttctacta gacgattata aaacatattc attaatttgc tgttggcaat
    15661 aaattgactt ttataatagg ccgactggat ctttttgatt actccttctg ctttgatgac
    15721 tgaaaataat gcaggccatg ctttgacggt tatttcgtcg ttttgtttaa ctaatacgcc
    15781 aggtgtgaag ccgactggag aaaggaacac aaaataaccg ataccggtat tttcatctaa
    15841 aggctgaaat tcatcaaaca accatttgcc tatgtacagc tcttcatctg gttctgttgt
    15901 ctcattttga tttgctatga ataatttagc taattctgct tcaagaaatt gaaggtcttg
    15961 atccttgtcg gtattttcct gctggctgat caatcgaaag acttcttctt taatcgtata
    16021 tttatcttta cgtgtaaaat tcatagccac accttccttc cttactctaa ctataacgga
    16081 atttcttcaa aaaaggtatc tgtacgacga aaaacagcat aatttttttt catatctctt
    16141 ataaaaaatg caaggtctgc tcctcaagct agaccttgca cttttcagct ttcttttgtt
    16201 tgtgacaaac cttcatggat cgtttcaata ttccatttca tcatctgata gtaagaatct
    16261 ccttgttctc cttcttttgc tatggaatca gtgaacagtt tatcatagat tttcaaaccc
    16321 gtttcttttg ctacccgttc catgcttctt gagtctacac tggtttccac gaataaaacc
    16381 ggtacttctt ttgctcttat ctgatcaatg atcgctttca tttgatcagg cgtaccttga
    16441 ctttctgtat tgatctccca tatataagct gctggcaaat catatgctcg tgaaaaatac
    16501 ttgaatgccc cttcacttgt tactaataat tttttgttct cttcgataga agcaaaactt
    16561 tccttcgctt gtgtatctaa ttcttttagt ttttccacat atgcttctgc atttttttta
    16621 tagagtgtcg cattttctgc atctttttca gagaatatac gtgcgatttc ctccacatat
    16681 tttattccgt tagataggtc tagccaagca tggggatctg cttttgtatg ttcttcaccg
    16741 ctagttaaat aaagaggttc tacatttttg ctaactgcaa aataatcttt tccttcttct
    16801 tttttagccg tttccatcaa gttatcgaac cagctgttac ctgtttcaag attcaaaccg
    16861 ttgtataaaa taacatctgc atcgcttgcc tttttgatgt cttctggtag tacttcatat
    16921 tcatgcggat ctgttccgac tggtacgata ctgtggatat ctatttgatc tgttccgacc
    16981 tcttttgcca tgtccgccaa gatcgaattg gtagccacga ctgacaactt ttctttactt
    17041 ccttcatccg cttggtcaga agcctgcttt ccgcaaccgg ctaataccaa gaaactaact
    17101 gccaagatta aaaagagtga tttttttgtt ttcatctatc tgcttccttt ctgaaataaa
    17161 attccttttg ttggagaaaa taaaaaagca aggataaaaa atgcggtaca tgctaacacg
    17221 atcgtagctc ctgatggcca attgtaacta taactgaaaa acacaccgac aactgcactt
    17281 agcataccga ttcctgcagc tgtaatgatc attttcagta aatggttcgt tagcaagtaa
    17341 gccgtggcgg ctggtgtgat caacatggca atcaccaaga ttgttcctac tgtctgtaaa
    17401 ctgactacag ccactaatgt caaaaagaac atcaaaagat aatggatcag cgatgtgttc
    17461 agcccataag cttttgccat tgtcggatcg aatgatgtga tcttcaattc tttataaaag
    17521 aaaaagacaa atatcaagac gattccacta acgagtgctg tcagcaacaa atcgctttcc
    17581 ctaacagcta agacattccc aaataaaata tgatacaaat ctgtcgcact ctgggcatat
    17641 gaaatcaaga taatccctaa tgcaaaaaag gagctaaaga caattccaat cgctgtatca
    17701 tttttcaatc ggctccgctg agtcacgaaa ccaatcactg ctgctgttag catcccaaaa
    17761 gcagtggctc caaagatgta actaaaacca aacatataag aaatggctac acctggaaga
    17821 actgcgtgag agatagcgtc tcccatcaaa gacatccctc gtaaaataat aaaagaaccg
    17881 atgactccag aagcaagtcc tacaagcatg gaagtgacca aagcgtattg caggaattga
    17941 taattcatta atccatcaat aaaggattgg atcatttttt ctctcctcct tttactaaat
    18001 gtctgatgat ctcaccatag gccaattgaa ggttcttcga tgtaaagact tcttgtactg
    18061 gaccggaagc gatcagctgt ttatttaaaa gaataacctc atcgaaatat tcttctactt
    18121 tatgaagatc atgatggacg atcaaaatcg tttttcctga attcttcaat tcctgcaaga
    18181 tgtcaaagat ctttcgttca cttaacgcat caatccctac gaatggttca tctaaaaaga
    18241 tccattctgc tccttgggct agagctcttg caataaaaac tctctgaagc tgtccacccg
    18301 atagttcgct gatctgtctt ttggcatatt cttccatccc tacttttttc aaagcttgtc
    18361 ttgcacgttc tttttctttt ttcccaggtc tttgtccaat tcgtaaagat ggatatgttc
    18421 ctaaaagtac aacgcctaat acatcaattg gaaaggaaag atccaattca cttcgctgtt
    18481 ccacgtaggc tattttttta cggactgagt ttaccggctg tccagaaaag gtgactttac
    18541 cagtagtagc tggtatcaac tctagcaaag atttcataaa tgttgatttt cctgcaccgt
    18601 ttggtccgat aataccggta attttttgtg gatttatcgt caagttgata gttttcaaaa
    18661 cagttttccc ttgataagcg gctgaaaggt cttgaattac aatggccgta cgttccaaat
    18721 cttacactcc tttttaggtt tacctaactt tttatttaaa tatagtttaa ctaaaatgga
    18781 aagcattttc aagtagaatg atgaaaaagt ttttttcgaa ttttcctcgc attttatgaa
    18841 aattttctgt agaatcacat caagagttca ataaggctaa cctaataatg aaaaacatgt
    18901 gttgaaacgt ttgattattt tttcttgttg actcaacaac gaaaggagtt tttgtatgga
    18961 agaagaaaag ttaactcgaa gtcttagtgc ccgtcatatc caaatgatcg cactaggtgg
    19021 aacgatcggt gtcggtctgt ttatgggtgc ctcctcaacc atacgttgga cagggccctc
    19081 agtgatggga gcctatgcga ttgcaggatt ctttttgtat ttgattatgc gtgctttagg
    19141 cgaaatgctt tacgttgatc cttcgactgg ttcttttgca aattacgcga gcgagtacat
    19201 ccatcctgtg gctggctact tgaccgcttg gagtaatatt tttcaatata ttgtcgtagg
    19261 aataagtgaa gtgattgctg taggatctta tatgaattat tggtggcctg atttgccggc
    19321 aattattccc ggaatcattg tcgtcttgtt tcttatgctg gcaaatctta tttctgtcaa
    19381 ggcgtttggc gaattagaat tttggttttc gatcatcaaa gtcataacga tcattttgat
    19441 gatcattgca ggtcttgggg taatcttatt tggatttggt aatcatggtc aggcagtagg
    19501 gatttcaaat ctttggaaaa atggtggatt cttcactgga ggcgtcaaag gatttttctt
    19561 tgcattatcg atcgttgttg cttcctatca aggaatcgaa ctgatcggaa tgactgctgg
    19621 tgaagcagag aatcctaaaa agacgatcat tgaagctgtc cagtccacta tcggtcgaat
    19681 cttgattttt tatattggtg ctatttttat cattgtgagt atctatccgt ggaatcaatt
    19741 gagtgagatt ggttcacctt ttgtacaaac tttttctaaa gtcggcatta catttgctgc
    19801 aggactgatt aattttgtcg tgattacagc ggcattatca ggttgcaatt cagggatttt
    19861 cagcgcaagt cgtatggtct atacacttgc cataaatgga aagatgtcta aaaaatttct
    19921 gaaactatct cgtcacggtg cgccttttta tcctgtcgta gcaatctcag gtggaatctt
    19981 aattggtctg atcttaaatt atcttctacc aatgttcatc aaccagtcag aaaatatttt
    20041 cgttttcgta tacagctcaa gtattttgcc tggtatggtt ccttgggtag tgattttgat
    20101 cagcgagatt cgcttcaaaa agaagcacgc agataaaatg catgatcatc cttttaaaat
    20161 gccactatct ccttatacga attatgtgac acttgctttt ttagctatcg tattgatttt
    20221 catgtttatc aatcctgaaa cgagaatctc attgattatt ggactcgttt tcttgcttta
    20281 catgacgatc cattactttg tacgcgaaaa aagaaccgga aaataaaaaa agaacgaagc
    20341 ttcaccttag tggaagcttc gttctttttt attgctttta tcaattcaat tttagcgtct
    20401 caccgggagt caggaaataa ttctccatgc tcatcccatt cagcgacaag atcgtttcta
    20461 ctggtacgcc agttcgttcg gctatttgat ttggaccttc tccttccaaa actacggttg
    20521 tcgcagcctg tgaagtcgcg ctgttggtat tagtttcaga ggaatctgtt gtgtcttgtg
    20581 ttgttcgagc ttgcatttca gaagcttcta ccgactctgc tgtctcagaa tcagaagatg
    20641 ttgtgtcttg tgtgcttgtt gactctgctt gaagagaggc gctagtctgc aaataaatat
    20701 cggaaatctt agcgacatga ttcgtataat tagccgataa ttgattgatc aaagtatcta
    20761 agtctacacc gtctttattt tctgctgcct gatacgctgc acgtaatttt ctgatccctt
    20821 cgtcggagat tgcttgaagc tcacgtgctt tttggttagc aactgctgat aaaccagcta
    20881 ttccctcttg atttccctga atttctgtct ggtattcttc aatcaatgca ggcgtctttt
    20941 cttcgatctt tttcgaataa tcatttacga ttgcttgaag ttgctctggc aaaccatttg
    21001 ctgttttttc ttcagaagtt tgtgtctctt ttgttttagt tgatgactca cttgtttgtt
    21061 tcgtctctgt tttctttgtc gttgtttccg ttatggtcgt acttttcgct tctgacgaat
    21121 tttttccatg aaaatataga cccgcaactc ctcctgctat gatcaaaaac acggcaactg
    21181 ctgccccaat catgatcatc cgcttacgag ctgcctgttt ttgctgtctt cttcgggtca
    21241 tccgaacatt ttctccccac tcattttcat catacatttt tttcaccacc aatttttatg
    21301 tataatagcg ttatcaaacg cttcatattc ttattgtagc aaataattgt cgctgtgcac
    21361 atttttatct ttgttattta taagaaaatt tcaccagttg agatgattcc aagattgtcc
    21421 gtcgatttct ccttgcagct gcattgttcg ttcgtctatc tcagccatca gtttctttat
    21481 ttcatataag agcgcacggt cttgatattc tgatttttcg cttaattcat ctaattgttg
    21541 gatcagccaa tcatttgtca taatgaagtt cctttcattt cttgcagtgt aaaggtcaag
    21601 tcttcgatct gctcttttgc ctcagtcagt gcttctacga tatatgcttg tttttcagat
    21661 tcttcttcct gcattttctg attttcctgt actctttgaa taaaccagtc aatccgctga
    21721 gtcagcgatg gtttgccttc caatgagaga tactgctgat aagcaattag cttctcccaa
    21781 cgtttttcct gggataaata cataaattgt gctaaaacga atggcggaag ataggtcata
    21841 tggaatttat ttgcgattcg ctgatacggc ctcaaaaatt cggaaatcgt atattcttct
    21901 tttcctccag cttggtattc tttttccgcc acacctgttg tcaatacgat tcctagttct
    21961 ttccctttta atacatgtgc tgctcgttcc aagacattat cttgccaaat ttttaaatga
    22021 gcaggagaac tataccagta aaacggaaac tggaaaatga tccgatcatg ctgggccaag
    22081 agctgtagtt cagtctctat atctattttt ccgtctggat aacttgattc caaatgatgc
    22141 caagtagctg gtaaaccagc agaagcttct ttgaaaaatt gctgatttaa agattcttcg
    22201 atcgttggat ggctaatgat gataagtgta cgcatccctt ttcctccttt ttagtcttcg
    22261 ctttacagtg tagcataacc gccacaagtt tcttttgccg atgcttgtca ttttaaccag
    22321 actaatcagg ttgtccaaga aactgcggtt tgtttttcca actgtttttt caaggtatcc
    22381 aaagcaatat tcttaaaatt ttcgtttttc atacgtttac gatagttcgt agccgtttct
    22441 ccggtactct ttttgaacat tcggctaaat acgactagac tattgaaccc tacttgatag
    22501 gcaatacttt ccacggattc atccgtcaga gaaagaagaa acgcccctcg attgatccgt
    22561 accccgatca aatattcctt gatactggtg cccatatgat ttttgaatac tcgcgacaaa
    22621 tggctccgat tgaccgctaa atgctgggcg atttcctcaa cagacaattt ttcagcataa
    22681 tgtgcctcga tgtatttgac tgtttcaaca gctaaagagg aatgaacctt ttgtacacct
    22741 ccgctgacct ttccgccatc ttttaaaagg actgataaaa aacggtaggt taatgctgtt
    22801 aactgtaatt cattttccaa ggtttcttcc gtatctttca tgcattctaa aataatgtcg
    22861 atatattttt gtatgtcaga agagaccatc gtgtattggt cttctctgaa aagcgactgc
    22921 cgaatgattt cttctgcagc actcccacca aatgaaagcc agcaatacat ccacggatct
    22981 tctccatctg ataagtaaaa cgtcgagtct cctggacgaa tcaagaacag atcacctttt
    23041 ttcaattgga attgttgatt tttcacatga taagatcctt tgccttccat cacgacatgc
    23101 aggatgtaat cattgcgaat agcaggacca aaagaatgaa acggcatcgt tttagagaaa
    23161 ccacaaaaat caaaatacaa ttctttttct acttgttgat gttccttttt taaaaaaatc
    23221 gattgctcca cgtctatccc tccttactcc tttagtataa tgcaacaaaa agttaaataa
    23281 aagtcacaga aatatattaa aaaggaagcg atttcttgtt aagtttaagt cattaaaaga
    23341 tctttgttat tcgttgtaac cggtttactt agtattatat attgaacagg agaggaatac
    23401 agattatgaa aagcaaaatg atccaacgtg tgtcctacgc tttaggcgct tttggacacg
    23461 acacattcta tgttacatta agcacttatt ttatggtatt cgtgacaagc cagatgttcg
    23521 caggaacaga taaggcaacg aatgcaaaaa tgattgctac agttacaagc ttagttgtag
    23581 gtatccgctt agttgaaatt atttttgatc ctttgattgg tggcatcatc gataatactc
    23641 gtacaaaata cggtaaattc aaaccatggt tagttatcgg cggcgttgtt agttccgtac
    23701 tcttggtagc aatctttacc aatttctttg gcttagctac tcataatacg actttattta
    23761 tcatattatt tgcaatcgtt ttcatcttat tggatagttt ttattcattt aaggatatcg
    23821 ctttttggtc aatgattcca gctttgagta cggaaacaaa ggaacgtgaa aaatttgcta
    23881 cgtttgctcg tttcggctct tctctaggtg caaatggtac tactctagtc gttgtcccta
    23941 tcgttacttt ctttacctat cttgttactc ataaacatat tgaaggcgct tcaggctggt
    24001 tctggttcgg tgtgattgct gctgttattt caggcggtac tgcgattatc actgccttgg
    24061 gcacaaaaga agtcgatact gcgatccgtc agcaaaatga aaaagctggg atcaaagatg
    24121 tgttcaaagc agttgctcaa aatgatcagt tgatgtggct tgcccttgct tatatcgctt
    24181 atgcgattgc caatgcagca acaactgccg tattattcta cttcttcaaa tatgtacttg
    24241 gccaaccaac tgaattttgg atcgttggtc tagttgctgc cttacttggt ttagtagctg
    24301 ttccactatt cccaatgctt acacgcgtca tctctcgtcg ctacgtttac cttggaggga
    24361 tcgcaatgat gatttcttct tactttttct ttacgatcgc aggttcaagt ttgccaatcg
    24421 ttatcatcgg gctagtattg ttctacttcc ctcaacaatt gattttctta tccgctttga
    24481 tgacgatcac agactctgtt gaatatggtc aatggaaaaa tggtatccgt aacgaagctg
    24541 ttactctatc aattcgccct ttactagata aaatcgctgg tgctttgtct aacgggatcg
    24601 tagggtttgt cgctatcgct tctgggatga ctggtaatgc tactgctgct gatatcactg
    24661 ctcatggcgt aacgactttc aaagcttatg cattctatgc tcctgctgct ttgatgattt
    24721 tctccgctat tatttttgct tggaagatta cattgacaga gaaaaaacat gcacaaatcg
    24781 ttgagaaatt ggaaaacaaa ttggctccag ctgatacaat ggaagattaa cttcacgatg
    24841 cagtagttcc ttcccattaa gaaaggatct tgaaaatgaa gattacagaa aaagattttg
    24901 gccaaggtta tcacttgata accttggaga acaaaaacaa gctagcactt tctatctctg
    24961 atttaggtgc tagaatcgtc agtttaaaat caaatgatcg agaattagtt ttgggatttg
    25021 acaccgctga agaatatatc gaaaaagatc cgtatatcgg tgcttctatc ggtcgcaccg
    25081 cagggagaat cgaaaatggc cgcttttctc tcaatggaaa aacttaccaa ttagccacag
    25141 atccaaaaac tggacataat ctgcatggtg gtgctcctgg atttgaatta aaaaaatggt
    25201 cttatgtcat tttgaatggc gaaaatgaag cttctgtcat ttttactact acaagtcccg
    25261 acggagaaca tggttttcca ggtacaatgg atgtggaaat ccgctatact ttgacaaaag
    25321 ataatatctg gcgagtgacc tctcgcggca ccagtgacca agatacttta ttcaatccga
    25381 caaatcatgt ttacttcaac ttgacgggag atgcttctca gtcaatcgat caacatgagc
    25441 tttggctcaa tagcgaagct tatgctcctt tgcgaacaga tagtattcct ataggggtaa
    25501 aagaaaatgc tgcaggaagt gctttcgatt ttcaaatacc taaaaaatta gcgtccgttt
    25561 ttgctagtga tttggatcaa aaaaatcttg ttgacggaat cgatcatcca tttttcttaa
    25621 aagaaaccgg cttgggtaaa gaagccgctc gcttgactag ccccgataaa agaatccaag
    25681 tagatattgc tacagatgct tctagtgtgg ttattttcac tgcaaatttc ggcacagaaa
    25741 caccagaaat gcgcaaccga aaactggcac atcacggtgg catcacattt gagactcaaa
    25801 cagctccagg tgcagagcgc ttctcagcat ttggttcgat tcatttaaaa gccggatcag
    25861 tatttgaaac agtgacagaa ttcaaaatca aaacaagaaa ggaataactc atcatggaaa
    25921 tcaaaagaag cttaaacaca aaatttgccg aaatattcgg acctaaaaaa actacacaat
    25981 acttctcacc aggacgcatc aatttgattg gtgaacacac agattacaat ggaggccatg
    26041 tcttccccgc ttccatcact tatggaacgc aaggtgtagc agcacctcga gacgataata
    26101 aaatattagt ttattccaca aactttgacg atatcggtgt cattagtttt actttagatg
    26161 agctagaata tgataaaaaa gcaaactggg caaattacgt aaaaggaatg gtccttaaat
    26221 taaaagaagc tggacactca atcgatcatg ggtttgagtt attagtggaa ggaacaatcc
    26281 ctaatggtgc aggactttcc agtagcgctt cattagaatt attagtaggt gttgtattag
    26341 aagatctgtt tgatttaaaa acagatcgct tggaactagt ccaaacaggt aaaaaagtag
    26401 aaaatgaatt catcggtgtc aattcaggta tcatggatca gtttgccatc ggcttcggtg
    26461 agctagatca tgcaatctta ttagatacaa atacgctgaa atacgaaatg gttccagtga
    26521 aactagatgg ttatgcaatc gtcatcatga atacgaataa gcggcgggaa ttggcagatt
    26581 caaaatacaa tgaacgccgt agcgaatgcg aagaagcctt ggcacgtttg caaaaaactt
    26641 tgcctatctc ttctttagga gacttagatg aagaagaatt tgaatcaact attgaccaaa
    26701 tcggcgatga tacattgata cgcagagctc gccatgccgt ttctgaaaat cagcggactt
    26761 tgaaggcaaa agctgaatta gaagctggga acttagaagc atttggtcaa ctgttaaatg
    26821 attctcacca ctctcttcgc tatgattatg aagtaactgg gatcgaatta gatacactag
    26881 tagatgctgc tcaaaaacaa gaaggtgtct taggtgcacg aatgacagga gctggatttg
    26941 gtggctgtgc gattgctctt gtcaaagaag aaaatatacc tgccttcaaa aacaatgtct
    27001 atgatgaata catgaaagtt gtcggctatg ctccggaatt ttatgttgca cacattggaa
    27061 atgggacaac aaagatcgat ccagaagctg ttcatgtttg ataaaaaaat caacgatctc
    27121 agctaaaaac aaatagataa aaataacctc tcagcaattt ccaaatgtga gccttttgct
    27181 cgtatttgaa aattgctgtt ttttattgac acggaaagag aacgtgtgtt ctaattgtgt
    27241 tgaagaggtg attgttatgt gggcgaatca aaatccggta tttgtttatg aaaaagaacc
    27301 ttcaagagat attttatgta ttgactgcaa atctttttac gcctccgtag agtgcgttga
    27361 aaaggatctt gatcccctta caactaaatt agtagtcatg tcctatccgt ccgattcgac
    27421 agaaacacgg ggtagcggat taatcttggc ttccagtcct gcagcaaaaa aagcttacgg
    27481 catcaccaat atcagccgtg ccagagatct tcccttcccc tatccaagtg atttatatat
    27541 tgttgcccca aggatggcct attatatgga aaagaacaga gaaatcaatt ctatctataa
    27601 aaaatatgca gacgaagcga accatcatgt ctattccgtc gatgaaagct tccttgatgt
    27661 aacaaatagc atggcactct ttggtaaaac aaatgcgtac gacatggcta aactgatcca
    27721 taaagatgtg tttgagcaga ccggaatcta cacgactata ggaattggcg ataacccgct
    27781 acttgctaaa ctagccttag acaatggctc aaaagacgga aagcaaatgt ttgctgaatg
    27841 gcgttatgaa gatgtaccag aaaagttatg gaaaatctca gatatcactg acttttgggg
    27901 aatcggcaaa cgaacagcaa aacgtctgaa acaaatgggt atacattctg tttatgattt
    27961 agctcacgcg gattattatc acttgaaaag caagctaggg gtcatcggta ctcagcttta
    28021 tgcccatgct tggggcatcg atcgaagttt tttgggacaa gaatatacac caaaatcaaa
    28081 aagtatcgga aatagtcaaa tcctagatag agattataca agacgagaag aaatcgagat
    28141 cgttatcaaa gaaatggccg accaagtcgg cactcgcttg agacgatctg cagcaaaaac
    28201 acaagtagtc agcttatggg taggatactc tttaggctat cttgatccaa atggcaagac
    28261 cgggtttcac cagcaaatga aaatcgaagc gacaaatgct agcagagatc tagcagatgc
    28321 tttattgacc attttccacc agcattacca ctaccaggat attcggcgtg taggcgtgaa
    28381 ctgttccgac ctcgtatacg cgacaggttt acagcttaac ctttttgaag atcctgaaaa
    28441 gcagataaat gaagcgaaag ttgattttgt tgtcgatacg attagaaaaa aatacggctt
    28501 tcaatcaatc atccatgcgc atagtctctt gcaaggagga cgcgccatcg cacgtagttc
    28561 tctcgttggc gggcatgcag gtggtatgag tggattagaa ggtggaaaag atgagacgga
    28621 cagcaaaaaa attcattgac tataacgagt atcgagatcg gccatttgga ttaaaatggg
    28681 ggactgcttt tgcgatggaa gaactagtca acggtatcaa aaaaaatgaa gaagaagcac
    28741 gcaaacagcc tcttcctaaa caacaaatga cattgcaaga aatcgatagt atactgacag
    28801 aagccttttt atctcgtaaa gcagtaatca ttcagctcaa tttaaaagat gaatttggaa
    28861 gactcttgga tgaggtcgaa gggcacttca ttggtgaagc ctatgaagat tactttatga
    28921 ttgaccatca gccgatattg tgggaagacg tacggtatat tgagttaaag gaaacaaaaa
    28981 aatggtttgt gacatgagag gttgtgacag aagtgatcaa cttcaaaaaa taagaaagga
    29041 tttttcgaaa attgcttctt aaatttttgt ggaattttgg cttatttttc gaagaagcaa
    29101 cttctggaac aacgtatatt agatatcaga ggttgtgaaa aagtcgctta gttttgagta
    29161 acaagccaag gcgctgattt cacaacctct gtctatctat ccatctattc ggtaagattc
    29221 atgcatgacg tcttctttat ttagtatgta gagccctttt tcttctgacc attcaacata
    29281 aatcagctgt tttacatctg catagccttc tttcttcaag ttctccataa gccatgcttc
    29341 agtcaattga ttggactgaa gcactttatg ctgaatttgc ccttcatcta caagcaggat
    29401 cgataaagct ttgtcttcat ctgatttttt caatacactc atttgaccgg cattctctag
    29461 gataacatgt ttggcatttt caagaaaaaa gcattcttgc tgtcttaaga ttgctcgcaa
    29521 ctgctccatc tcgatatggt tatttgtcaa ctctgtcatg ttgatgacgc cgtctttgat
    29581 tagaacagat ggctcgcctt tgacccaacg attgaccttt tcattttttt ggacgatacg
    29641 ttcgatacca taaatcatta ctgcccaaag cgcaatggca aataggacat gccctacatg
    29701 gacattgtca tcataggttg attcttctaa gatcccacct aaaacaagcg tatagactaa
    29761 atcaaacggt gtgatttctg acatggattt cttcccaagc aaacgtacca ctaataataa
    29821 accgatcaac ccgaacacca gtttcattgt aattgaaaaa atcatttttt ctccccctaa
    29881 ctatctgtta attaaattat atatgctttc tcacgaacac gcatggaata tacaaggaag
    29941 aaatgtatcc ttttactaaa aaaaggactg tgacactgta gtgaccccca aaagttagac
    30001 ttttttggag tggagaaatc cactcctttt ttttatgctc taaaatgata tttttacaca
    30061 accgttacgg ttgttttacg aaattggacg agactttgcc agtttaattt ctgttttatc
    30121 cgttcattat tgtagtaata gatatagcta tcgatggctg attttaattc ctcgaaacag
    30181 ttataaactt tcccgtgaaa aatctcttgt tttaataagc caaaaaaatt ctccataagt
    30241 gaattatcta agcagttccc tttccgagac atgctttgaa agattttgta ctccttcaat
    30301 ttgtttctat aagctttcat ttgatacgcc caa
//
LOCUS       DACDXE010000031        29886 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.031, whole genome shotgun sequence.
ACCESSION   DACDXE010000031 DACDXE010000000
VERSION     DACDXE010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.031"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..662)
                     /locus_tag="IWM23_001411"
     CDS             complement(<1..662)
                     /locus_tag="IWM23_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAP9884061.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VK"
     gene            complement(662..967)
                     /gene="gatC"
                     /locus_tag="IWM23_001412"
     CDS             complement(662..967)
                     /gene="gatC"
                     /locus_tag="IWM23_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAP9884062.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(1073..3109)
                     /gene="ligA"
                     /locus_tag="IWM23_001413"
     CDS             complement(1073..3109)
                     /gene="ligA"
                     /locus_tag="IWM23_001413"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAP9884063.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(3126..5360)
                     /gene="pcrA"
                     /locus_tag="IWM23_001414"
     CDS             complement(3126..5360)
                     /gene="pcrA"
                     /locus_tag="IWM23_001414"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAP9884064.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            5515..7074
                     /locus_tag="IWM23_001415"
     CDS             5515..7074
                     /locus_tag="IWM23_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAP9884065.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(7185..9116)
                     /locus_tag="IWM23_001416"
     CDS             complement(7185..9116)
                     /locus_tag="IWM23_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9884066.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(9134..10048)
                     /gene="pfkB"
                     /locus_tag="IWM23_001417"
     CDS             complement(9134..10048)
                     /gene="pfkB"
                     /locus_tag="IWM23_001417"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAP9884067.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(10045..10797)
                     /locus_tag="IWM23_001418"
     CDS             complement(10045..10797)
                     /locus_tag="IWM23_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9884068.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(10957..11166)
                     /locus_tag="IWM23_001419"
     CDS             complement(10957..11166)
                     /locus_tag="IWM23_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884069.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(11177..12505)
                     /locus_tag="IWM23_001420"
     CDS             complement(11177..12505)
                     /locus_tag="IWM23_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884070.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(12870..13457)
                     /locus_tag="IWM23_001421"
     CDS             complement(12870..13457)
                     /locus_tag="IWM23_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAP9884071.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(13540..14790)
                     /gene="clpX"
                     /locus_tag="IWM23_001422"
     CDS             complement(13540..14790)
                     /gene="clpX"
                     /locus_tag="IWM23_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAP9884072.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(15037..16326)
                     /locus_tag="IWM23_001423"
     CDS             complement(15037..16326)
                     /locus_tag="IWM23_001423"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAP9884073.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(16480..17412)
                     /locus_tag="IWM23_001424"
     CDS             complement(16480..17412)
                     /locus_tag="IWM23_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884074.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(17569..18228)
                     /locus_tag="IWM23_001425"
     CDS             complement(17569..18228)
                     /locus_tag="IWM23_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAP9884075.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(18231..19256)
                     /gene="sppA"
                     /locus_tag="IWM23_001426"
     CDS             complement(18231..19256)
                     /gene="sppA"
                     /locus_tag="IWM23_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAP9884076.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(19269..19559)
                     /locus_tag="IWM23_001427"
     CDS             complement(19269..19559)
                     /locus_tag="IWM23_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884077.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            19968..21773
                     /gene="glmS"
                     /locus_tag="IWM23_001428"
     CDS             19968..21773
                     /gene="glmS"
                     /locus_tag="IWM23_001428"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAP9884078.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(21845..23200)
                     /locus_tag="IWM23_001429"
     CDS             complement(21845..23200)
                     /locus_tag="IWM23_001429"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAP9884079.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(23224..24393)
                     /locus_tag="IWM23_001430"
     CDS             complement(23224..24393)
                     /locus_tag="IWM23_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884080.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(24390..25277)
                     /locus_tag="IWM23_001431"
     CDS             complement(24390..25277)
                     /locus_tag="IWM23_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAP9884081.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(25466..29158)
                     /gene="nifJ"
                     /locus_tag="IWM23_001432"
     CDS             complement(25466..29158)
                     /gene="nifJ"
                     /locus_tag="IWM23_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAP9884082.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            29357..29878
                     /locus_tag="IWM23_001433"
     CDS             29357..29878
                     /locus_tag="IWM23_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9884083.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
BASE COUNT         8124 a         6490 c         5388 g         9884 t
ORIGIN      
        1 tctttcaccg tacgggtcat tggtccgatt tggtcaagag atgaagcaaa ggcaatcaaa
       61 ccaaaacgtg agacacggcc ataagtaggt ttcatcccta cgatgccgtt aaaagctgct
      121 ggttggcgga tacttccccc agtatcacta cctaaagaaa ctggaatttg tccagctgca
      181 actgctgctg cagaaccacc agaagagcca ccaggtactt ttgttgggtc ccaagcattt
      241 tttgttttct tgaaataaga agtttctgta cttccaccca tagcaaattc atccatgttt
      301 agcttaccaa cagggatcat atctgcttga tagattttat ccatgaccgt tgcatcataa
      361 attggttcaa aattgtaaag gatcttagat gcagcggttg tcaagatatc ttttgttacg
      421 atattatcct tgataccaat tggaatacca gcaaggacgt tgctttcatc aatccctttt
      481 tcatctaaag cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat gaatgcctcg
      541 acctcaggct ctgtttcttt gattcgttca aatgttgttt ttgtcaaatc ttgtacagtg
      601 atttctttcg atacaagcat atgatgcagt tcttctagag aacgattgtg taattctgtc
      661 attatgcgcc agcctcccca ttatcgatga ttgcgggtac tttgataaag ccgtcttctg
      721 tttccggcac atttttcaat aattcatcgc ggttccatcc agcttgcgcc cgatcttgtc
      781 gcatcacgtt tactgtttca accacattcg acgtaaatgg aacaccttcc gtatctactt
      841 cttccagcat ttcaaccata tcgatgattt tacctaattg gtcggtaaaa cttgcaagtt
      901 cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt tcttcactaa
      961 tcgccatact accccttctt tctaaacgag ttaaaaactt tgctctctat tttaacacaa
     1021 gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa aaaactcatt tttcattctt
     1081 ctactgagcg acttttagcc aatgcatctg ccatttgatc ttctgtccaa acttcgattc
     1141 ctaattcttg tgctttggtc agcttgctgc cagcatcttc tcctgctacc acgatatccg
     1201 tttttttcga tacgctacca gtcactttcc cgccaagatt ttcgatcgtc tcttttgctt
     1261 cttcacgagt aaatcgtgtt agtttaccag taaggactac tgttttttct ttaaaaggag
     1321 actcgacttc ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg acgcctgctt
     1381 gttttaattc attcatcaat tcatggactt cttcgttact gaaataagtg actacactgt
     1441 cggcaatgat atcaccgatc gtatcaagcg caataatcga ctcatagtca cttttactca
     1501 atgtttctag atcaccgaaa tgttctgcta aaatttttgc tgctttcgct ccgacatgtc
     1561 ggattcctaa cccgaaaatc aaccgctcga ccgaattatc tttgctgttg tcgattgctg
     1621 tcaaaatatt atttgctgat ttttctttta ttttgtccaa cgtcaataac tcttcttctg
     1681 tcaatttgta aagatcagca acatctgcta ctagtttttt atcatacatc tgttctagta
     1741 cgcgcggccc caacccatcg atattcatcg catttcttga aacaaagtgg ttcaaacctt
     1801 ctttcatttg agcagggcat ttaggattga tgcaccgtaa ggctacttct tcatccaaat
     1861 ggacaagttc acttccacaa actggacaat gtgtaggcaa ttggtattct tcgctgtctt
     1921 taggtcgttt atctaacacg acttgcgaaa cttctgggat gatgtctccc gctttataaa
     1981 tcagaactgt atctttcaag cggatatcct tcaacttaat ataatcaccg ttatgcaggc
     2041 tggcacggct tactgtcgtt cctgctactc ggactggtgt catgatagct gttggcgtta
     2101 ctacccctgt tcgaccgact gtccattcga tgttttcgat tagcgtttca acttcttctg
     2161 gcggaaactt gtaagctgct gcccatctcg gtgctttgat agtaaaacca agttgatctt
     2221 gtagcgagaa ttcattcact ttgatgacga ttccatcgat ttcatagggc aaatccacac
     2281 gtttgtcatg atattcttca atgtatgacc acacttcatc aatcgaatga catagacgtt
     2341 tttcccgatt cgtgtggaat ccaatttttt ctagctcttc tagcgcatca tactgggttt
     2401 ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa agtgtccaga tttcttttcg
     2461 ctgtgatttt agaatctaac tgacgcaaag aaccagctgc ggcgttccga ggattcgcaa
     2521 agatatcttt tccctctgct tctctgtctt ggttcaattg gacaaatgac cgttttggca
     2581 tgaaacattc gcctcttact tcgatgttca ttggttcttt taatttgatt gggaccgacc
     2641 ggacagtttt taggttttca gtgatgtttt ctcctacagt tccatccccg cgagtcgcac
     2701 ctctcacgaa atttccatct tcataacgca aagaaacgga aagtccatct atttttagtt
     2761 cacagcaata gtcaactaca cgtcccactg ctttttgtac acgttgatca aaagctatca
     2821 gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga gtgacttttt
     2881 caaaaccttc taatactttt ccgcctactc gttgagtggg agaatcagac gtgatcaaat
     2941 caggatattg ttcctcgatt gctactaatt ctgcgtattc tttatcataa acatagtctt
     3001 ccactgttgg tttatcttct acgtagtatt cgcgagacca ttggttcagt cttgtacgaa
     3061 gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc atttaccctc
     3121 cactactaag ctttttcaat tggcgcaaag gcagccaaaa gacgttttac tccttgactt
     3181 gggaaagcca catctagttc tagatccttc gacgtcccgc ttactcggac gactgttcct
     3241 agaccccatt ttttgtgttt gactttatct ccaggtttcc agctgttatt ttcccctcca
     3301 gtagcacttt tagagggaac aaccgctgtc tctggctgtt tatagctata tgctgttttt
     3361 ttagcgaata caggcgtttt tttaggcgta ggacgcatgc cttcgatttc cagtaactcc
     3421 tgatcgattt cttctacaaa acgagaaggt cggttatatt gcgtccgtcc atataatgtt
     3481 ctggagaaag catttgttaa atacaacgct tcttctgctc gcgtgatccc cacatatgcg
     3541 agacggcgtt cttcctccag ttcgctttct tccatcaatg ctcgagataa aggaaaaacc
     3601 ccctcttcta gaccaatcag gaatacaact ggaaattcta atcctttggc tgcatgcaaa
     3661 gtcatcaatg tcacttggga agcatcttct tctaaattgt caatgtcgga aacaagtgcc
     3721 agatcattta agaagaccgt tagtttttct tctggtgcat cagcgtcttc ttcattttgc
     3781 tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa gttttcgatg
     3841 cgtgcttgtg cttctaatgt attttggatt tttaagtctt ctagatatcc gctacgatcc
     3901 aaaacctctt tcgtcaattc tgtcactgtc aagtatggga tcatctgagt gacttcttgg
     3961 atcatctctc caaatgcacc taattgctgt cctgcttttc caccgatatt tgccagatcc
     4021 acattttgcg ctgcttcaag cagtggccat tcatgaagag aagcaaaact gcgtagtttt
     4081 tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc aaaactgatt
     4141 gaatcttgag gatttaccag cacattcaaa tacgctaaaa tatctttgat ttctttacga
     4201 tcgtagaact tgtgccctcc aaccatttta taaggaatat ttgctttcaa cagagtttct
     4261 tccattacac gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc gtaattccga
     4321 tgattgcttc gaatctcttc ttgcatccgg tctacgataa aacgggtttc gtctcgttca
     4381 ttatctgcac ggtagtaagt gatcttgttt ccttctttgt tttctgtcca caagtttttt
     4441 ggttttcgat tgctattgtt ttcaatgact tggtttgctg cgctaaggat gtttttcgtg
     4501 gagcgatagt tttgttctag caaaattact gcagcatctg gataatcttt ttcaaaatcc
     4561 aagatgttct gcatatctgc accgcgccag ccgtaaatac tttgatctgc atctcccacg
     4621 acgcacaggt tcctaaatct acctgccaat aaattgacta gcgtatactg tgcgtgattt
     4681 gtatcttggt actcatctac atgaatataa tggaatttgt tttgataata cgtgagtgaa
     4741 tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa gtccatacat
     4801 tgattatatt gaagctcttt ttggtaagca gcataacatt ttgccgcaat ctcttcgtaa
     4861 taactgcctt gcatttttgc aaaatcctga ggggtctgca ggctgttttt tgcttgagag
     4921 attgttccta atatacttcg aggatcatat tttttcggat caatgttcag ttcttttagt
     4981 atccgtttca tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt ccggttatac
     5041 ccgataaaat caacatctct tcttaaaata cgcacacaca tcgagtgaaa agtcgatacc
     5101 cagacatctt cgcctccaga tgcgagaatc gcattgacac gttctttcat ttcttttgca
     5161 gccttattgg taaaagtaat cgctaggatg ttccaaggat tgacttcttt ttcctcaatc
     5221 aaataggcaa tgcggtgtgt cagcactctc gtctttccac ttcctgcacc tgccattagc
     5281 agtaatggtc cgtcagtgtg aagaacagct tctttctgac gggggttcat cccgtttaat
     5341 aattcagctt tggatgacat taacaacatc ctttctttta tcaatcttgt taattatagc
     5401 attttcttgg ctttcattaa actagctagt tcaaaatcat ttgtttcttt ttatggattt
     5461 ttccttgtca attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt ttgaatggaa
     5521 aaaaaaggaa aaatcaagaa attagataag actcagatat attttattct aaaaggaata
     5581 tttgttggac tgctgacagg aatcgtcgtt agcctgtttc gattatctat cgaaaagcta
     5641 agcgatttag ttcgaagtat ctatgaaatg agtcgggaac agccgattta cttaattggc
     5701 ctagctattt gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa aaatgaaccg
     5761 gatatcaagg gaagcgggat tccacaagta gagggacagc ttcgcgggga attatcaatg
     5821 aactggttct ctgttttatg gaaaaagttc attggcggtg tactttcagt tggtgcgggc
     5881 ctttttctag gtagagaagg accttctata cagcttggcg catccattgg acaaggagct
     5941 ggacaattgt ttcggagtcc atcttcagaa gaaaagattt taatttctag cggtgcaagt
     6001 agcggacttg cagcagcatt caatgctcct atcgcaggat tactctttgt tttagaagaa
     6061 atccatcact ccttttctcc acttgtttgg ctgacatcat ttgcttcagc aatcactgct
     6121 aacttcgtct ctctttactt tttcggcttg agacctgttt tatatctagg tgaccttcct
     6181 tctttgccgt tgaaatatta tggttcctta gtgatcttag gagtacttct aggcatctta
     6241 ggttttattt atcagaatgt cttgctggct ttgccaaaat tttacaaccg attgccttta
     6301 ccagcttatt ttcacgggtt cgttcctttc ttgctgattt tgccaattgg ttatttatgg
     6361 cctagtactc taggcggagg caatcaaatc gttttagctt ttggacatgc ttccttgcca
     6421 atcattatga ttatcggttt gtttgttctt cgattcgttt tttccatgat ttcttacggt
     6481 gggggtcttc caggggggat tttccttccg atattgacat tgggggcttt aatcggtagc
     6541 ttttatggaa acattatggt tgatttcggg atggatccaa tctacatcaa ggattttgtc
     6601 gttattgcca tggctggtta tttcacagcg attggaaaag caccgctgac agcaattatt
     6661 ttagtgacag aaatggttgg ctctcttaat catctgatgc ctttaggatt ggctgctctt
     6721 gttgcttata tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc attattggaa
     6781 cgtctacttg gaaatactca gaccgcctca ctaaaaggtg caaggacaac ttttgatttt
     6841 ccagtaacgg cagagagttc tttagacgga ttaatggtac gagacttcaa ttggcctaaa
     6901 aatatgctgc taatctctat acgcagagga aatcaggaaa tcttgactca cggtgatacg
     6961 atcatgaatg taggagatat tttggttatc ttgactgatg aagcaaactt gccaagaatc
     7021 aagcaggaaa tcgaggctaa atcttctatc acaaatttga agcaatcatt ttaacgaaca
     7081 tggaaaaact attttacaac aactattcat ctacactact tgattctata tgattctata
     7141 aaaaaagaag aggctgggaa aacccagcct cttctttttt atttctatgc ttctgctact
     7201 gcaggagctt ttctcaataa accataaatc acaccagata cgactgctcc gatagcaata
     7261 aacaccaaat acatgaccgg gtgactcagt aagaatacaa cgaaaatccc gccgtgagga
     7321 gccagtaatt tgattccgaa agctccgact aatccgccag ttaaagcaga accaataata
     7381 aaacttggaa tggcacgaat cgggtcagct gctgcaaatg gaatcgatcc ttccgtgaca
     7441 aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt gatctgtttt gctgaattta
     7501 ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag gtaccattcc tccagccata
     7561 acagaagcca tcacgacact tccgccagtt gcaacagtcg tagctaaagt tgctgtacca
     7621 aacacataag cggctttgtt gattggaccg cctaaatctg ccgccatcat tccagctaat
     7681 aatgcaccaa gtaaagcagc attcgaacca ctcatacttg tcaagaaacc gttcaatgca
     7741 tcattgattg ctttcatagg gacattgacc agcaacatca agaaaccagt aatcagtaag
     7801 ccaaacactg gataaaagag gatcgtttta attccatcca atgattttgg taagttcttg
     7861 aataatttac gtaaaaatac gatgacataa ccagctaaga atccaccaat cagtgcacct
     7921 aagaatcctg caccgcctgt attagctaat gcaccagcag caaatccaac gatcaaacca
     7981 gggcggtcac cgatacttga agcaataaat cctgctaata ccggcagcat gaaaccaaat
     8041 gcagcttgtc cgatttggtt gaaccagcta gcagcttgat tgtaattccc tagatttgat
     8101 aattgatcat gtggcacgcc aataaattgg tcgatcatga aagaaagcgc aatagcgatt
     8161 cctccaccaa taacgaaagg taacatgtgt gatacgccgt tcatcaaatc tttgtaaatt
     8221 ctttgtccga tagtaccatc cgcatttcct tcttctgaac tgctttcttt tccatcacca
     8281 tggaatactg gtgcagtacc actaagggct agattgatca actcttctgg tttacggata
     8341 ccatcactga caggacgatt gatcagttct tttccgtcaa agcgattcat ctctactttt
     8401 ttgtctgctg caacgattac accttctgca cgggcgatgt cttcagcagt caatcggttt
     8461 ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca cacccatttc tttcgctttt
     8521 ttcttcaaag catcttctgc catgtaagtg tgagcgatcc ctgttggaca agctgtaacg
     8581 gcaacaacga attttctttc tggatgagca gaagcttctg cagcttgtgt ttcttgttct
     8641 gctttttctt ctgcttcttt ttgctcttca gctgtttgga acaagtcttg gacttcttct
     8701 ggtgtatttg cttcttttaa ttttcctacg aaatctgcat caatcagcaa tcgagatagt
     8761 gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac
     8821 agataagttg gctgtccgtc taaggcttca taatctactc ctttttggct ttttgcgaat
     8881 aaaactgttg cttctttaac tgcactgttt ttcgcgtgag gcatggcgat tccatcacca
     8941 agacctgtag aagtttgtgc ttcgcgggct agaattcctt ctttatatgt ttcgatatcg
     9001 gaaatccggc cggcatcaaa cattttttgg accatttcat cgattgcgcc ttttttatca
     9061 gtcgcttgca aatccatgat catcgcttct ttgaccatta aatccttgat attcatgatt
     9121 atcctccttg aaactaatca atttttgtga tttcaacttc tggcagcaac tcatcaatca
     9181 attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct tccgcaagca actccccatt
     9241 taaaggcttc gattggatct ttcgtacggc tgtaagcccc aataaagcca gcaatcattg
     9301 aatcccctgc acctactgaa tttttcaatg ggcgaactaa tacattcgtt ttataagttc
     9361 cttgttccgt gaagagcaaa gcaccgtctc cagccattga gatcagcaca ttctgtgccc
     9421 cttcagctag taattttttc ccatacggaa tgatttcttc tacagattgg aaggttgtgt
     9481 tgtaaagctc agctaattcg tgattgtttg gtttgatcaa caacggtttc ttaacaagtg
     9541 cattttttag gtcatcacct gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga
     9601 tatcaatcag ttcttgataa aatccttttc gcaggctagc tggcgcgctt cctgacaata
     9661 cgacgatatc tccttgtcct actgttccta atgcttgctt caactcatcg atttcttcat
     9721 ctgaaataga tgggcctaag ccattgatct cagtttcagt gtctgatttt agtttgatat
     9781 tgattcttgt atcttctttt acggttgtga acaaagactg gatttgttct ttttcaagcc
     9841 acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat
     9901 cgatccgttt caaaatacgg gaaacattga tcccttttcc tccaggtaac ttgaagtctt
     9961 ccttcattcg gttcaaatcg cctagtttta acccatctac gcgtacgata taatcaattg
    10021 atggattaag tgttactgtg taaatcattg tattgcctcc ttaattgttg tcttttcacg
    10081 gaattcatta acgaattcta atggacaacg ctcggttatg atcgtggctg cttccaaagg
    10141 tgcaacagag gtgaaggaca actggttgaa tttggtatga tcaatcaata caaatgtctc
    10201 ttcggaattt ttcatggcta aacgtttaac agctgcctct tctggatccg gagtcgtgaa
    10261 tccttgttca agatgggcac cgttcattcc cataaatgct ttattaaaat gaaaattttt
    10321 tagttgttct atcgcattag atcctaaaac agcattagtt gccagcttta tcgttccacc
    10381 taaaataatg gtagcaagct ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt
    10441 cactactgtg atctgttttc cttgcagaaa aggaatcatt tctaacgtag tagagccagc
    10501 gtctaaataa atgacatctt cgtcattaat ggtttcagct gccgatttgg cgatttgctg
    10561 tttttcgtga acgtttttga gtgatttttc actcatattc tgctcaaaat tcaataattg
    10621 ctctttctta gctccgccat gaactctttc tagaaaccca ctattctcca gctcttgtaa
    10681 atcacgtctt atcgttgatt cagaagcatt caaaaggttt acaagatctt gtgatttcac
    10741 gattttctgc tgttcaagca agtctaggat tttttgtctg cgttcctctg taagcatttt
    10801 cacgatcacc tcaatacaaa taatagcata ttttttttct actagcaata taaatcagtc
    10861 aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaaa taaaaaacca gtcagaaaga
    10921 ctggtttttt atttttttgt ctatcatcaa gccgttttat cgttttttat tttcgcttct
    10981 tgtttttttc atataaacag aagcaggttt tctttcctta tgaagaagat aataataaag
    11041 tcccatcccg ccaaacatga ccaaaaaacc gatgactgat cgaataggaa aattaggaac
    11101 aaaaaagaga cagacggata ataatgatgc cggaaccatt attcgataat agagttgttt
    11161 atccattttc ttcacctcat attttttgta aagtaatcaa ttgttctaat tctcggtaat
    11221 ctaaaaattg cacacgcaga tgctgctcac ctttcttgac atcgaattgg atacttcgcc
    11281 gctttttctt tattcgtttg aaggtaacat cagagagtct atgtttgatt tcctttaacg
    11341 gtaattctga cagggattct gaaggaacat agattcttaa ggataaaaga tactcttgat
    11401 atttttcatc tgttacctct acttctagat attccctaat ataataacaa taagcttcga
    11461 ttccattatc caaagcaaca gtcggatcga taactttata gcacatcatg cgtgatcgat
    11521 tctcgtcaac ccaaggaaaa tacgcctgag catttagtgt tccgcgaaca cgcctttctt
    11581 tataatctcc ttgttggata tcataacttt ttgttacaaa aaatgaagca ttctttttag
    11641 gaacaggttg catttgtgca ttctgatact ggacagattg gatcgtatgg tcagtgtaaa
    11701 tgtattcaac gaatggcggt ttcaataaac cgacaccatt gtcccaagca agatgatcaa
    11761 acccaagctg gtccctaaaa taggattgaa acatttgatt gtttggcttt tgccagttaa
    11821 aggggtctcg ataaagctgg atacatatga cttcgttttc taattccttc actttttcta
    11881 cttgttggat gtcgagaaaa atcggtttag ggatgatttg atgataatag cgactataat
    11941 acatttccca acacgaagtg aaacataaac cttggtaaaa aagatcgtat cctggaaatt
    12001 ggttgcaatc gactactact tccccgtttt gttgtttcat tttaggtaaa tgttcgattt
    12061 catccttttt ctcaaacaat agccgttgag agatctgttt tgtattgtga tagataaact
    12121 cgctataagc tcgcatatag gcgaacaccc catgagcaat catttttttc tgaatgaagt
    12181 ctcgaatcaa aaattgcttt tctttgaaaa cagagataga caatacattt tttatcaata
    12241 attgattatc ttccaagcga aaactacatt cgttctcgtc atttcctaca actaaccaac
    12301 tttcgtctct taccgatgct agcattattt tttcttgaag ctttcgaaga tcatacggat
    12361 ccgcttgttg tgtgaaagag cggataaatg ttggctggat caacagacgt gtcaaaatag
    12421 cccaatcgct tatttcctgt tttttttctt cttcatttaa gtctgtgagc tggatagtca
    12481 tcaataattt atcttcgcca atcattttct cacctctttc aaatcttttt ataatttaat
    12541 tataaccaat gataaagaaa aactaaaact ttatgtctta gaaaccaatt tggacgatta
    12601 catgatagac taagtaaaaa gaaacaaaga ggtgtttaca atggggttga aatttgaacg
    12661 tacagataat ttagataaac ttttcgaatc atttgctgtc gatccggaaa aaacagttgc
    12721 accaaaagaa gagatcgatc gatttctaaa accacagcca caaaatgaaa agaaaaaaga
    12781 caagaaacaa aagtaagtta agctcaaaaa aagacttacc agccaaactt ttatgaaaga
    12841 gtttgactgg taagtctttt ttattctttt tatgattcaa ttgcttgttc gattgctgcc
    12901 cacgcttctt cttttccctg ttttgtaacg gaagaaaaga gaataaacgt gtcattgcga
    12961 tcaaaatcca acgctttttt gatttcgctc tcatgcttgt tccatttacc acgagggatc
    13021 ttatctgcct tagtggcaac aacaattaca gggatatcat aatacttcaa aaattgatac
    13081 atctggatat cttcttgaga aggcttatga cgcaaatcta ctaaagaaac gaccgcacgt
    13141 agctggtctc tagtcgttaa atatgtctcg atcatctttc cccattttgc tcgctcagtt
    13201 ttggatactt ttgcataccc atatcccgga acatcgacaa aatgtaatgc gttctcgatc
    13261 aaataaaaat tcaatgtctg ggtctttcct ggcttaccgg aagtacgggc taaattcttt
    13321 cgatcaatca atgtgttgat aaaagaagat ttccctacat ttgatcgtcc tgccagagct
    13381 atttctggca attgtgtatc tggatattgt tttggtgcca ctgcacttat gataatttca
    13441 gcttgatgga ctttcatggt ctctctcctt aacaaggtga tgaataaagc aagcggctgt
    13501 cgcaaaagcc aaaatgactt ttacggcagc ctcttttcat caacccgctt ttttctctga
    13561 atctttatag attactgttg gctcaccaga caattcagct gcttcttttg tgataatcac
    13621 tttttcgatg ttttcatttg aagggatatc aaacatcaca tccatcatga tttcttcaat
    13681 gatggaacga agtcctcgag caccggtatt gcgttcgatt gcttttttcg caatggctct
    13741 caacgcttca ggttcgaatt caagttcggt attatctaga gaaagaagtt tttgatattg
    13801 tttgactagc gcatttttcg gctcagtcaa gatccgtacc agatcatctg ttgtcaattt
    13861 ctctaaagct gccataacag gtaatcgtcc gataaactca gggatcaatc cgaattttag
    13921 taaatcttca ggaatgatat gctgcatgac actttcgtct tcagaaagtt tttggttttg
    13981 tgtaccaaat ccaatcgtct tttcgcccat acgattcttc acaatcgttt cgatgccatc
    14041 aaatgcacca cctacgatga acaatatgtt cgtcgtatca atctgaataa attcttgatg
    14101 tggatgttta cgtccgccct gaggtggcac acttgcaaca gttccttcta gaattttcag
    14161 caaggcttgt tggacacctt cgccggaaac gtcacgagta atagatacat tttcgctttt
    14221 acgcgcaatt ttatcgatct catcaatata gatgatccct ttttctgcaa gttccacatt
    14281 gtaatcagca gattgaagca atttaagcaa aatgttttct acatcttccc ccacataacc
    14341 agcttctgtc aaacttgtcg catctgcgat tgcaaaaggt acattcaatg tttttgctaa
    14401 tgtttgtgct aaaaatgttt taccagaccc tgtaggtccg atcaagcaga tattgctttt
    14461 ttgcagttca acatcatctg tgttttcttg tgcttgctga ttgacacgtt tatagtgatt
    14521 gtatacggca acagctaacg agcgttttgc gcgatcttgt ccaatcacgt agttattaaa
    14581 tacttcaaga atttctagtg gtttagggac ttcagtgaat tcgcgtacag cttcttcata
    14641 aaattcttca tcaatgattt ctttacatag gtcgatacat tcattacaga tgtagacccc
    14701 tggaccagct acgatctttt tcacttcttc ttgggttttc ccgcaaaaag aacagcgtac
    14761 ggtctcattg ctacttggat tgtcatacat ggtcttcacc ccttaacttg caaaaagtat
    14821 gcaagaaact atcaaagact ttacagtctt ttactatcat aacatagttt agtaaaaaga
    14881 aaaagtgttt aacaaaggat caactgacct atacctttgc attttttact cttaacaaaa
    14941 aataaggaac tgtgacaaaa tcgtgtcaca gtcccttatt tcgaataaac aattttctag
    15001 aagtagcttc ttcagaaatg atttattact tgatttctat tctttcgctt ctgtcgctgt
    15061 acctgtgatc aattcgattg cttttttcat tgtcacatca tgttccagca tatcttctgt
    15121 caacacgcgt ttgatttgtt cgattggcat gttgtatgtt tctgataatt cttggatttc
    15181 tgcattgatt tcttcttctg ttgcttgaag attttcagca gctgcaattg cttcgatcac
    15241 aagatttgtt cttgtgcgca tttctgcttc accttcgaat tgtttatgca agtcttcttc
    15301 tgtagaacca gttaattgat agtacatttc aggagagata ccttgacgtt gcatgttgtt
    15361 caagaattcg tccattgaac gatgtacttc atcatgaacc atcacgtgtg gcaattcaac
    15421 gatttctgcg ttttcaacag ccatacggat cgctgcttca tcttttgcat cgtccgctgc
    15481 tttttctttt gtttctacta attctttgcg atatttttct ttcaattcgt ctaatgttga
    15541 tacttcgtca tcaacatctt ttgcgaattc gtcatctaat tcaggcactt cttttgcttt
    15601 tacttcatgg attgtcactt tgaagactgc ttcttttcca gcaagatctt ctgcttgata
    15661 atcttctggg aaagttacat ttacatctag actttcacct gatttatgac caactaattg
    15721 atcttcaaaa cctggaatga atgagcctga acctaattca agtgagtaat tttcgccttt
    15781 tccgccatca aacgcttcgc catctacaaa gccttcgaag tcgattacta ctgtatcgcc
    15841 attttcagct gcagcatctt ctttgataac taattcagct tgtgcttctt gttcacgttt
    15901 gatacgtgca tccacgtcag catctgttac ttcacggtct tgtttttcaa caactagatc
    15961 tttatattgt cctaatttta cttctggttt cactgttact tcagcagtga ttacccagtc
    16021 ttgacctttt tccatgcttt caacatcgat ttttggttga gaaacaggat cgatgccagc
    16081 ttctttgaca gcagcttcat aagcttctgg taatacagca ttcaatgcat cttcatataa
    16141 agcttcttca ccgtacatac ggttgaatac ttgacgagaa actttccctt tacggaatcc
    16201 aggtacgttc aagctttttt tcactttatt aaacgcagtt gttaatcctt tttggatttc
    16261 gtcttgcgca atcgtaaatt ttaaaacacc atcattcgtg cctttttttt caaatgttgc
    16321 agtcatttaa ttccctccga gtttagaaat tcattttata catcaaaaac gtttcatgca
    16381 tacctttaaa tagtacacta accctttcaa attgtaaaca tattgaatgg atagaatcct
    16441 tgtttttcat aacattttta tctttttcac tgataaaatc taatggaaga agatcatttg
    16501 ctgcaaaatt ttttctcttt tttttaaaaa atcttgtttt tcatcttctg taccttcata
    16561 catcgttttt cctgtacacg cctctacagt cagttgaaac cagcgcttcg ggtcttcgat
    16621 gtatgtatca tagacaggat aaacaattgc agattgcaac gtgaattctt cttttaacac
    16681 ggaactaagc agcgcatctt cattcccgta acgatctgaa aatacacata attgctggta
    16741 acttgcctgc tgctgcggct ttggcagatc ttttggtatg actttgacta acttttcttc
    16801 aatcgtcaaa tagaaaacag gatggcattc gttcaattgc ctaagtgtct ccagtaattt
    16861 tgcccttgct aaaatatgga cctctggcga tacaataagt ttactcgctg tttggataaa
    16921 ctcatgataa ggaagctgtt cgatctcatc aatagacgca agctgacgtg caggttccaa
    16981 cgaaggaagt tcctccgatt tattgtggag tgccttgatc ttctgcctct ccatttgtcc
    17041 ggataattct tctgcaaatt ggattttttc aatgacccgc tgctcgaaag cttcataaaa
    17101 atctgtactt gctatcaatt ttctagcata taaaaaatct tgtgtactga ttaacaagtc
    17161 aaaataaaat tcagcaaact cctcattttg aagatactgt ttttcatgca agagtgcttg
    17221 cttcaatgct tcttttttct cgtccaactc gtaaagacaa aaaacaagca ggctattagc
    17281 ctcaaagctt tctttcaact cgtaagctcg ttgaaataat tcttttgcct gctgatattg
    17341 ctggtttgct agtgcttgtt gtgccactcg cattaaattc tcatattttt ctggaaaatc
    17401 aatcggcttc acgcagtgtc ctcctcttgc tcatattcag ctcatgttca atagtagcaa
    17461 aacaaaaaaa gcatgacaat taaaaataac tgaagcaccc tctaaaagct agattttaga
    17521 tctgactttt agaaggtgct ttcaactgtt actgcttttt attgctcatc agctgttgac
    17581 ttcctggttg aacgctttga tcagattgat cgtaacaaca cttgtattag tgaaaaaatc
    17641 gcccagatgt tgctttttct gattaaagac tgctggaaga taccctaaca tcagcaaagg
    17701 aaattttaga atgaaccggc aagcaccttc tctaactaaa acggttgacc agctaagttc
    17761 ttcttcatca aagctgatga ctcgaatacc aaagatcatt ttcccaatcg tttggccatg
    17821 attgaatttc gtcaataaaa taaaatacgc tagataaata agtaaggctg tgaatccgta
    17881 gagactcaga taagaatctg aaaaattaag ccaacctaga tttttggcaa taccaagtgt
    17941 taaacgcgta atagctccga tacacaaaag atcgaccaag aaggcaaaca ttcggataaa
    18001 aaaacctgca tagaaatact taggaaaatc atttgctttt ggcggttcat ttcttgtgtc
    18061 cgtatacttt ttccatttac gttgtttctt tttgatttct tcgggcgtca aaggctctct
    18121 ttcaaaagag ctcagtacct tttgggaaaa gttggagtca ggtcctacag caggctgctg
    18181 ttgcttatct ttttcttgtt ccttattttc atttgaaagc tctgacatgc ttactcacct
    18241 ccgtaataat acattgcttt tggtgattcc gctgtcccta gattctcaag aacggataag
    18301 atctgactag tctcagaagg cttcaatccc tggaattctg ctagtttact tcctaaccat
    18361 gtactagcaa acccagatgg cgtggaagaa tattctacta gttccgcatc ttcaagctgt
    18421 tgttcctttc gtatagcttt taaagcatct tcaggaaatc ccaattcatc gactaaacct
    18481 acttcttttg cttggacacc atcataaatc cttccatcag caattttttt gacttcttct
    18541 tcagatttat tccgtccctc gctgacgatt gctacaaaac gactatatgc gctgtcgata
    18601 tatgcttgaa gcaccgcttc ttcttctttt gtctctggtc gcgttcctga acccatatct
    18661 ttcaacgctc cgcttttaac tgttgtatct tccacaccca gtttttctag aagtcctgaa
    18721 taattcaatc ctgacatgat cacaccaatt gagccggtca ctgtttcttc tgttgcaaag
    18781 attttatctg cttgagcaga aatatagtag ccaccgctag ctgccatgtt tttcatgctg
    18841 acatacattg ggatatcatg ttctttacga atcgtatcca atttcttagc gatttccgca
    18901 ctttcgtaga caccgccacc aggagaattt acttctaaaa agataccttt gactgttgga
    18961 tcttcttcaa tcgccttcag ttgttccata aaatttttat gattgtaacc ttcagaagca
    19021 aataaacctg aagaccctcc cgcagcaatc gttccatcta ctgataattt tacgattttt
    19081 tgcgtactgt ctccctcttc caacacattt ggagctagtt cttcatcccc atataaccaa
    19141 ctgttcaatc cctcaatttg atcgttttcc tgaggcttag tcaaactagc tgaaactaac
    19201 gaaaccacaa ataatcctgc agcaatcaga actgctgccc aacgtctttt attcattctt
    19261 tctcctttct aatacaagtt ttttatgacg atcacagttt ctgactgctc agaagtgtcg
    19321 aaccgctctg ctttttcttc ccgcgcatac ccggaaaacg actcttttgt taattcatct
    19381 tctaccgtaa actcacgagc ttgagcaact tccaaggcat caggtgcgat ttcttggatg
    19441 ctatcccagc cagtatctaa aaatattttc gtatcatctg atacaccagc tgatgcttcg
    19501 aatttggaca attgtttttt taacattccc agtgtcatga tctcaatttt tgaatccatt
    19561 tttactcctc ctctatccga ttactttcta ttgtaccgaa ctcctgaaaa agatgataga
    19621 taaaaaccca ttattttttg acataaaaag gacatttgac aaagaatttg atatatacca
    19681 atttattttt tattcttttt ttgttgacaa gaacattcgc aaaaggttat gattttaaat
    19741 gtttccagat aaaaaacaaa aaggaggata gttatacctt ttaggatagc gccaggtctg
    19801 tgtaaacaga tgacgaggag agggtttatc gaaagattcg gcggatggcc ctagggactg
    19861 cactctacag aaaatccaca aaaaatgtct gcaaagataa aacaaaagga ttttcctgca
    19921 gctggaagca ttcatacgaa ttcattatag aaagtaggat gttttttatg tgcggaattg
    19981 ttggaatgat tggattaaaa aatgtaacac caggattgat tgacggtcta gaaaaattag
    20041 aataccgtgg ttatgattca gctggtatct ttgtctcaga tggcacaact gattatctgg
    20101 tcaaagcaca aggcagaatt caaaatttga aaaacaaaat aaatattgat actacaggaa
    20161 atataggtat tggtcataca cgatgggcaa ctcacgggca accttctgaa gaaaacgccc
    20221 atccacatac ttcccaaagt ggacgcttcg tgctcgttca caacggcgta atcgaaaact
    20281 ttgaagaact taaaattgct tatttagcaa atgaccactt tattggtgag accgatactg
    20341 agattatcgc tcatctaatt gaaactttcg cgaaggatac tacaacgcaa gaggcgtttt
    20401 taaaagcttt gcgggtcatc aaaggttctt acgctttcgc tttgattgat cggacagcac
    20461 ctgatgttat ttacgtcgct aaaaataaaa gtccactatt aatcggactc ggcgatggat
    20521 tcaatgtgat tgccagcgat gcaatggcta tgcttgctca tacgaaggaa ttcgttgaga
    20581 ttgaagatga agagatggtt accgtcacat cagaaaaagt tattattcaa aattttgcag
    20641 gtgacatcgt agaacgttct tcattcgaag cacaagtaga tgcttcagat atcgagaaag
    20701 gtacctatcc tttttatatg ctgaaagaaa tcgacgaaca gcctattatc atgcgtcgta
    20761 tcgctcaaaa atacgtaaca gaagacaatc gtgtctctct tgatcaaaag ctggtcgata
    20821 cattgtctga tagcgatcga atctatatcg ttgcttgcgg aactagttac catgcggggt
    20881 tagcaggtaa acagactttg gaaaaattga cacagattcc tgttgaggtt catttagcca
    20941 gtgaatttgg ctataacaca cctcttcttt caaaaaaacc attctttatt ttcttgagtc
    21001 aaagtggcga aacagcagac agccgccaag tgctggtaaa aatcaaccgt ctcggctacc
    21061 cttcacttac gattacaaat gttgctggtt ctactctttc gcgggaagca tcctttacac
    21121 tgctattgca tgctggaccc gaaatcgcag tagcatcaac gaaagcttat actgcacaaa
    21181 tagctgtact aactttatta gccaaagcta tcggtgataa aaaagaattt gaaacttctc
    21241 ttgtattcga tatcactcat gaattgagtt tagttgctaa tgcgatggaa tcggtcatcg
    21301 cccaaaaaga ttatttagaa gagttggcag aagtatatct cagcgataca agaaatgcct
    21361 tttacattgg acgtggtgct gactacaacg tttctcttga agctgctttg aaattgaaag
    21421 aaatcagtta tatccaggca gaaggttttg ccgctggcga attgaaacat ggaacgattg
    21481 ctttgatcga agaaggaaca ccagtgatcg gaatcatcag tgaagaagtg actggtgcgc
    21541 atactcgagg aaacctgaaa gaagtcgaaa gccgtggcgc taaaacacta gttattgtct
    21601 cagagggatt agaaaaggaa agtgaccagc tggtgctacc aactgtccac ccttatctaa
    21661 ctactctaac cacagtcgta ccgactcaat tacttgcata ttacgcaacg ctattacggg
    21721 gattcgacgt tgacaaacca cgtaatttag cgaaatcagt gacagtagaa taataaaaga
    21781 acaaggacca gtgtcagaca tctgacactg gtccttgttc ttttattttt tatgaaaata
    21841 tctcttaatc cagtccgatt tcttctttga ctactgccgc gattttatcg acatagtaat
    21901 taactttctc atcagtaggc gcttctgcca tcactcgaag taatggttct gtaccggaag
    21961 gacgaactag gattcttcct tctgatccca tttcagcttc tgcctcttca atcacttttt
    22021 tgattgccgg aacatccatc gcaccgtttt tatcacttac gcggatattc actaattttt
    22081 gcggatagat tgttacctct gctgcaagct ctgataattt cttacctgtc tgtttcatga
    22141 tattcagtaa ctgaattcct gaaagcatcc catctccagt tgtgttaaaa tctaagaaaa
    22201 tcatgtgccc agactgctcg ccgccaaagt tataatcgtt tttgcgcatt tcttctacaa
    22261 cgtaacgatc accaacttgt gtaacgacat ctttcaaacc gattccttcc accgctttgt
    22321 ggaatcctaa gttactcatg acagtcgtaa cgatcgtatc ttttttcaat cgattttttt
    22381 ctgccaagta tttagcacag ataaacatga ttttatcacc gtcgacaatg tttccaagtt
    22441 catccaccgc aataatacga tcgccatcgc catcgaaagc tagtccagca tctgctcctt
    22501 tttcaacgac catctcagct aaacgttctg gatgggtaga gcctacgccg tcattgatat
    22561 ttaacccgtc tggatttgtg cccattgtat aaaaatctgt ttcaagatcg gcaaacaaac
    22621 gattaacact tgttgaagtt gctccgtttg ctgcatcgac acaaatggtc aaaccggaaa
    22681 ggtcaccgtt gatcgtttgt acaaggaatt gagaatattt caataagcct tctggaaatt
    22741 cttctacagt tcccaatcct tcagctgatg gacgaggcaa ttcatccact tcagcatcta
    22801 ataatgcttc aatttctagc tcttggtcat ctactagttt aaaaccatct gcaccgaaaa
    22861 atttgattcc attatcttgt gctggattat gagaagcaga aatcatgacc cctgcgcttg
    22921 ctttttgcaa acgtgtcaga taagcaacac ctggtgtaga gatcacacct agctggaaca
    22981 cttcgatacc caccgataaa agtcctgaaa tcagcgcttc ttctaacatt tgacctgaga
    23041 ttcttgtatc ccgccctact aacacgcgtg ggcgttttcc ttcttcttca tgctggctta
    23101 ggacataacc tccgcaccgt cccagcttaa atgcaagttc tggcgtaagt tccttgttgg
    23161 cttcaccgcg tacgccgtct gttccaaaat atttacccat agttgtaaaa ttactccttt
    23221 aacttaattt tctgaggaat ttgttctgct catttgtgtt gattcgcttg tttctgatgt
    23281 ttcttctgta cttgtggtcg agctgcttgt cccagattcc gaactggaag aaatggttgt
    23341 tgtcgatggt tccgttcctg aatcagcaga tgaaatactt ctactcgtac ttgtattttc
    23401 ttgtgtccga ctgctcgtcg aagcagagct actgcttgac gtttgcaaca cgggtgtaat
    23461 ctccacacta actgattcgg gcttgatact taacccctct tctactggta tttcaatttt
    23521 acgagttgtt cgatgattga tacctgagac attgatcggt acagcaatac tgtcaccgat
    23581 cgatgcaagc aaatcgctag agccgcttac ttctgcttcc agttttgaaa aactgaaatc
    23641 atagctttct accccggcag ctggtgttcc ttgctgtatc ccataaagtc gtaccgactt
    23701 gcttggcttg atcgaatcag ctctcactgt tacttgcact ggatcagaga cgatacttaa
    23761 tacttcccct gctgcattca gcgcctgtat tgggacctca ctattaatcc catcatctgt
    23821 gaccatcgaa gcgtccacat cagctactac tcgtttgatt tctgccaatg ttttgtcacc
    23881 tgtagtgata gagacttttt taggactaac ggaagtatca tcaagttcat atccagatgg
    23941 tgtgaacgtt gaaggaatca acgtttccac atcgaaatct tttattacct ttttctcaac
    24001 cgttactgta atagtcgatg gttcgatctt tgctgtgaca gcactgctca aattttgaat
    24061 ttttagcgga acttcatgtg ttccttcgcc cagcttagtg atatctgctg tgacgcggaa
    24121 catccgtgta tcttcgtttg cctctgcatt caactgaact cggtttgcac tggaaagttt
    24181 gacagtcact gcattttcat atccatggat atagtagcgc tttgaatcat aagaaatctt
    24241 gatcggcacc cccgtaactg tttgattata cgcttcattt ccagaaagcg tatttggaac
    24301 ggactggcca tttgcattga agtacagcgc aatactgaac agcaatgcaa taaaactgta
    24361 aagaatgctt gtccgtttct cttttgagat catttgcgat cccctccctt agtcataccg
    24421 tctaggaaag attgaatcac gtttttcttg ttttttgttt tctcttcagc aattagttct
    24481 ctacgcaaga ttgccaaata ttcttgctgg gataagccag ccatcagttc attatctaac
    24541 gtgatgctga cgtccccggt ttcttcagag acaacgatgg taatcgcgtc actgacttcg
    24601 cttataccga cagctgcccg gtgtcgagtc ccgaattctt tagggatcaa catactttct
    24661 gatagaggaa gataggcgct tgctactgcg atctttcctt cttttacaat aactgcaccg
    24721 tcatgcagcg gtgtattagg aataaagata ttgatcagca gctccccagt gatatctgca
    24781 tctaaagcaa tccctgtctc aatatactca tctaatccag tatgacgttc gatagtgatc
    24841 aacgcaccga tttttcgctt ggacatatat tggatggctt tgtcaaaaga caagatcatc
    24901 ttctcatctt cctgctgttc gctttttgat tgtttgaaaa gcgaactgcg tccaagatgt
    24961 tccagtcctc tccggatttc cggctgaaaa atcacgatag cagctatcac cccgtacgta
    25021 atgacttggt tcatcagcca agacaaggtg tgcagaccaa tgatttccgc tacgattcgg
    25081 ataacaataa aaatagctac acctttcaga agctgaatcg cttttgtgcc tcgcagcagt
    25141 tggatcagtt tgtatacaag ataccagaca accagaatat caagtatgtt caccaaaaaa
    25201 ttcagagaga acaagtcacc ggaaaacatt tgctgccaat agtccaaatt aaaaagttcc
    25261 ccgatttgaa atgacataaa aagcctctct tctcttttga gatttcctct ctagtatatc
    25321 atatgtttta attttagagg ctgatatgct cgtatttttc ttgagaaaaa cgcataattt
    25381 tcaagaaaat tgcaagtatg cgttttattt ttctctgcaa taaatatttt taaacttcag
    25441 agaattattc attatatttt ttctgttatt tttccttttt ttcttttgta gcttgttcca
    25501 tttttttacg tcgttctgct ctggcagctc ttctagcagc tcttctttct tctgcttcaa
    25561 ggtccgtcgt tttttctttt ctagtcttaa cgggtacatc gatagattcc tctggttcaa
    25621 gtcgtttctt gattttttct aattctcccg tcagtgcagc atagttgtaa aaacgtgttt
    25681 tagcgtcgtg gacagtcttt tcaaataaag cttttgcaag atttggattt gcgttatcta
    25741 gcgaggaaaa gcgaacttgt ttcctcatga aatcaggcat agcagagaaa tcaggttttt
    25801 tgtagtccaa agtcattggg ttcttgtttt tttcgaccat tgtagggtta tatcgatata
    25861 atgaccaata accagaagaa acagcttctt ttgcttcttt aagcgattct ttcattccgc
    25921 caagcaagcc atgtgtgata caaggcgtat aggcaataat cagagaagga ccaggatact
    25981 tttctgcttc ttcaaatgcc ttgatcgttt gcatttgatt ggcacctgaa gcaatttgcg
    26041 ccacataaac atttccatat gtcatcgcca tcattcctag atctttcttt gaagcatact
    26101 ttccactagc cgaaaattta gcgattgccg aagctggtgt tgcttttgag gtttgaccgc
    26161 cagtattgga atagacttcg ttatccaaaa caaggatatt cacgtctgct ccgctggcaa
    26221 gtacatgatc gatcccgcca aaaccaatgt cgtaagccca tccgtctcca ccaatcatcc
    26281 attggctgtt ttttacgaat agatcctttc tcttgtatat atcctccagt tcagggaaaa
    26341 ttgatcgttc gctttcgatc gcagcttgca gttttgctgc tctttgctgc gttccttcac
    26401 tctcaaacat gtgtactaac cagtcttcca tcaattctcg tagttcttct gatacttttg
    26461 gcaatacttc tttgattgaa cttgccaaag cctcgcgacg agtctgtgcc gctagaagca
    26521 taccataacc aaattcagca ttatcttcca ataaagaatt tgaccatgca ggcccttgac
    26581 cacattcatt ggtcgtataa ggagaagcag gagaagcacc gccccaaata gaagaacagc
    26641 ctgtcgcatt cgcgatcatc attcgatcac cgaacatttg tgtcaataat tttacataag
    26701 gtgtttcgcc acagcctgag caagcgcccg agaattcaag aagcggttga ttgaattgtg
    26761 tagacaagac agtattcttt cctttgactg gatttgcttt ttgtcttaat gtcattgcaa
    26821 aagcccaatt cacagcttgt tctttctgtt cttcataagg cttcatcact aaagcttttt
    26881 cttttgcggg acaagcctct acacacagac cacaacctgt acagtcttcc actgaaactt
    26941 ggatacggta ttttacgccg tctgcgcctc gcatttctct cacgataaat ccttctggcg
    27001 cttcttccat ttcctcttca tctgctaaaa atggacggat ggcagcatga ggacaaacaa
    27061 aggcacattc attacacatc gtacagcgat cactaatcca ctcaggtact tctaaagcga
    27121 cgccccgctt ttccatagaa gccgttccta aaggcatttc gccagttgtc attccatttg
    27181 ctacaagtgt accaacagag agttgatttc cttcctgtgc attcactggt tcaagtattt
    27241 cttgtatata accagtgttc cttttattga ttttttcagg catctccaac gttctccaat
    27301 ggtctgggat cgcgacttga tgaagcagtt cggtcgtccg atcgattgct tgtatgtttt
    27361 tttctacgat agccattgat ttatgtccat agcttttcag ggcttcttct tttagcagcg
    27421 gaagcacctc atcaaaagga atgatgtcag ctaatttaaa aaaagccgtt tccattgccg
    27481 tattgatccg gcgtcccaaa ccgacttctt gggccaatct catggcattg atcgtataga
    27541 atctaatgtt attttcagcc aagtaacgtt ttaattttaa gggcagatgc gtttctaatt
    27601 gttcgtcgct ccaaagagta ttcaataaaa aaatgccacc tggcttcaat cctttgacca
    27661 agtcatagct atgcaggtaa gctggtgtgt gacaggcaac aaggtccgca tgctcgacaa
    27721 gatacgctga acgtatcggc gtatctccaa aacgaagatg agagacagtc agaccgccag
    27781 attttttcga atcataataa aaatagcctt gcgcatattt atcggtgtga tctccaataa
    27841 tcttgatagc tgatttattt gctccaactg ttccgtcaga tccaaatccc caaaacttgg
    27901 cctggaatgt ttgcggttct gtcaaatcca gactttcctt tggttccaaa gattgataag
    27961 tcacatcatc cacgatccct atggtaaacc gttttttgcg tatgcttggg tcttttttca
    28021 attcatcaaa aactgctgag atttggtcgg gagtcacatc tttagacccc aatccgtagc
    28081 gaccaccaat aacagccggg cgaagttcag aatcatataa cgcactttga acgtctaata
    28141 agagaggttc tccacctgcc cctggttctt tacttcgatc aagaacagca attgttttta
    28201 ctgtatgagg caacttttgc acgaatgttt ccactggaaa aggacgataa agatggatat
    28261 tcaaaaaacc tgttttccgt ccgtttgcat tcaagtaatc aatcgtttgt tcaatcgtct
    28321 gtgcaactga tcccatagaa acaatgactt cttccgcatc gtctgctccg taataagtca
    28381 ccaagtcata agaagttcct cgtaatcggt tgatctcttt catatatttt tgaacgattt
    28441 caggtaattt atcataatag gggttgatcg tttcccgttg ttgaaaatgg atgtctggat
    28501 tctggttcgt accactaaca gtcggatgat tgggattcat ccctcgttta cgaaatgaag
    28561 ctaatttctc ctgattgatc agaggagcta attcttcata ttccaaaact tcgatttttt
    28621 gaatctcgtg actcgtacgg aatccatcaa agaaattcaa aaaagggact tctccttcaa
    28681 tagaagcaag atgggcaaca ggtgctaaat ccatcacttc ttgtacactg ctttcagcta
    28741 acatagcaaa tcctgtttgt cttgcagcca tcacgtctcc gtgatctccg aaaatattca
    28801 acgcatttgt cgtcactgcc cgactagcaa cgtggaaaac acttggcagt agttccccag
    28861 caatcttgta catattcggt atcatcaaaa gcagcccttg tgaagcggta tatgtagttg
    28921 tcagtgcacc agttttcaat gatccatgga ccacaccagc tgctcctgct tcggattgca
    28981 tttctactac ttttactggt tgaccgaata cgttttcttt tcctgctgct gaccattgat
    29041 ctactaattc agccatggtc gaactgggtg ttattggata tattgcagca atctcggtaa
    29101 atgcataaga aatatatgcg gctgccgtat tgccatccat tgttttcatt tttcgcatga
    29161 ttcatcatcc ttgttcatat tctctcttat tatctgttcc attataacgt aaaacatgcc
    29221 atttttttct tttatttata aaaatatttt acatgtatct atttgaattg gtcatctaat
    29281 ttttagacaa acagtctaaa tcgttgcctt tttttgtcag acatgtataa actataagcg
    29341 aaagaggtgt tttaacatgg atatatcagt gattgatgca acgaaaacaa atacacaaaa
    29401 aggaatcctt tatggttcat cgaatgctcc taaaaaaatg gtcgaattca tcaacttagc
    29461 ttgtccgtat tgccgacaat ggtttgaaga atcttatgag ctattagaag aagcagtcca
    29521 aagcgggcaa ttgcagcgag tgatcaaatt gttcgataaa gaaaaagaaa gcttacagcg
    29581 agggaacgtg atgcaccggt atctcacaat cagcgatggc caaaaagcga tcaaagagat
    29641 caaacaaata ttcgatactc aagacgagtg gaaacatctt tctctccaag aagtagcgga
    29701 ttttgcagta gataaactaa agctgacaga acaaaaagac gaacagctga gccaagctgt
    29761 catcaacgaa gctgagcaag cacatattcg atttgttcca actgtcatct tgggtaaaga
    29821 aatctttgat gaatctattt caatagagga attaaaagaa ttgatccaaa caaaataaaa
    29881 aaataa
//
LOCUS       DACDXE010000032        29531 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.032, whole genome shotgun sequence.
ACCESSION   DACDXE010000032 DACDXE010000000
VERSION     DACDXE010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.032"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(152..550)
                     /locus_tag="IWM23_001434"
     CDS             complement(152..550)
                     /locus_tag="IWM23_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAP9884084.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="IWM23_001435"
     CDS             complement(547..1320)
                     /locus_tag="IWM23_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9884085.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="IWM23_001436"
     CDS             complement(1317..2000)
                     /locus_tag="IWM23_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAP9884086.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="IWM23_001437"
     CDS             complement(2167..3054)
                     /locus_tag="IWM23_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884087.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="IWM23_001438"
     CDS             complement(3051..3920)
                     /locus_tag="IWM23_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884088.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="IWM23_001439"
     CDS             complement(4057..5343)
                     /locus_tag="IWM23_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9884089.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="IWM23_001440"
     CDS             5616..6194
                     /locus_tag="IWM23_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAP9884090.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="IWM23_001441"
     CDS             6194..7123
                     /locus_tag="IWM23_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884091.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="IWM23_001442"
     CDS             7120..8856
                     /locus_tag="IWM23_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9884092.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM23_001443"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="IWM23_001443"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAP9884093.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="IWM23_001444"
     CDS             complement(9752..10261)
                     /locus_tag="IWM23_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAP9884094.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM23_001445"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="IWM23_001445"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAP9884095.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="IWM23_001446"
     CDS             complement(11562..12653)
                     /locus_tag="IWM23_001446"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAP9884096.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM23_001447"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="IWM23_001447"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAP9884097.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM23_001448"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="IWM23_001448"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAP9884098.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM23_001449"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="IWM23_001449"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAP9884099.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="IWM23_001450"
     CDS             complement(16616..17590)
                     /locus_tag="IWM23_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAP9884100.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="IWM23_001451"
     CDS             complement(17571..19454)
                     /locus_tag="IWM23_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAP9884101.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="IWM23_001452"
     CDS             complement(19472..21697)
                     /locus_tag="IWM23_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9884102.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="IWM23_001453"
     CDS             21812..22846
                     /locus_tag="IWM23_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884103.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="IWM23_001454"
     CDS             complement(22913..23926)
                     /locus_tag="IWM23_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9884104.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="IWM23_001455"
     CDS             complement(24004..24192)
                     /locus_tag="IWM23_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAP9884105.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(24632..24760)
                     /locus_tag="IWM23_001456"
     CDS             complement(24632..24760)
                     /locus_tag="IWM23_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAP9884106.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="IWM23_001457"
     CDS             complement(24763..26136)
                     /gene="rlmD"
                     /locus_tag="IWM23_001457"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9884107.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(26247..27302)
                     /locus_tag="IWM23_001458"
     CDS             complement(26247..27302)
                     /locus_tag="IWM23_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9884108.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="IWM23_001459"
     CDS             complement(27312..28742)
                     /gene="gatB"
                     /locus_tag="IWM23_001459"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAP9884109.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(28739..>29531)
                     /locus_tag="IWM23_001460"
     CDS             complement(28739..>29531)
                     /locus_tag="IWM23_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     A"
                     /protein_id="HAP9884110.1"
                     /translation="AISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGV
                     APGVKEAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYG
                     YRSENVKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRD
                     FEKVFADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLS
                     EGLPVGLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
BASE COUNT         8331 a         6416 c         5284 g         9500 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tacaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccgc tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattgat tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc gcaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aactaaaaag actaagaaac aataacaaaa
    24601 gttaacactt cttagtcttt ctttagcacg tctatttatt aaagatttta gggccttttc
    24661 tcatgttttt acgatcattc ttaggtgttt ccatttgttt ggatcggcct ccaccttgtt
    24721 tctttttgat caattctttc attttttctt tttggtccat ttttattctc ctttaacgaa
    24781 tgaagcgact aattcgatat gatgggtttg cgggaaaaga tctacaggct gaactttctt
    24841 cacggcataa cctgcttctg caaataactt agcatctctt gcaaaagttg ccggattaca
    24901 tgagatatag acgattcgtt ctggtgacat ttcgattgct gattcaataa aatgatgatc
    24961 taatcctttt cgtggagggt caactacgat cacagatggt ttgatccctt tttcttgcca
    25021 ttttttcata actttttctg cttttccgac ttcataatga acattttcga ttttattatt
    25081 taaagcattg aactgtgcat cttcaattgc ttctggaata acttccatcc catacacctt
    25141 atccactctg tctgctaaag acaagccaat cgtacctatt cctgaataag cgtcgatcac
    25201 aatgtctttt ttatctaact cggcaaactc gatggctgtt tggtacagat actctgcttg
    25261 ttgagtattt acttgataaa atgactttgc tgagattcga taggtttttc ctaatagctg
    25321 atcttcgata tagtctctgc catataaaac tttttctttg tctcctaaaa tcacatttgt
    25381 tttttcttca ttgatatttt ggatgaccga aaccacttct ggcaattctt catgaatagc
    25441 ttcagcaatt tccttggatt taaagaattt ttctttacga gtaactaaaa cgaccatcat
    25501 ttcatgggaa taatgaccac gacggataat gatgtgacga agctgtcctt catttttttc
    25561 ttcattatat cctcgtactt ggaaccgttg caagatatct ctgactactt tgattgcttg
    25621 atcgatgaca ggatcttgga tataaaaatc ttcgataggg actagatcat ggctattctt
    25681 tttgtaaaaa ccggtggcta actgtccatc gatacggcga acagggatct gtgctttatt
    25741 acggtagccg aatggttctg ccattccgac cgttggaagt acaggaactt ctggcatctt
    25801 agcaatcttc gccattacat gctctacttg tttttgtttg aagacaagct gttgttcata
    25861 cttcaaatgg gctaaaggtg cgatacctat gcgaagtaat gcattgtttt ctacaggctg
    25921 acgatcaggt gattgtgtaa taaatgtttc gacttttcca tatccaaact tattgcctgc
    25981 ttttactact agtatttcta cttcttctcc agggagtgca ttttcgataa ataatggata
    26041 accatcgatt tttgctaccc ccattccttc ataactcaag tcgacgattt cgactgtata
    26101 tcgttcgttt ttctttacta tttgctgttc tgtcatattt tgctccttta agcctatttt
    26161 tcaatcctac tgaactctac tataaatcag ctaattacac aactaaaacc ggcactgacc
    26221 ttactacgtt cagcaccggc ttttgattaa ttcttttgat taggtgtttc gtctgctatt
    26281 tttccgtcac cgtcgatatc ttcatccgtt aaacgttcga cttcttttac gaattctttg
    26341 ctgacaacct ctaattcttc ttcatctgta cctgtgattg cttcatcagg aatctgatcg
    26401 gtattggcaa agaactcaat gtgttgatga agattttgga aatgcatcgg tgcttccccg
    26461 ccgtattcac catctaagtt gatcattact ggtcgattga tatcatcttc tgtttcaacg
    26521 aacaaactgc ttgttttggt atagattatt cttggatcat cgacatgtcg accgccattc
    26581 agcatgagtg ctgcaatatg caagatctca aagatatttg ctgtcttcac aatgattaaa
    26641 gaaaacttcc catcatccaa tttggcatct ggcgcgatct gttcgaatcc tccaacagag
    26701 ttcgtcagcc ctaggaaaaa catcgaagca tttccgatat attccccttc atcatacttc
    26761 aagcgcattt tgattggctt tactcgaggg agcatctctg ctcctttcgc aagataagca
    26821 agataaccaa aaatactctt tagttctgat ggaacttcat aagtcaattc tgtcaaatag
    26881 ccacctgcag cgatattgat gaagtaactg ttatcagaaa ggccgatgtc catttttacg
    26941 gtttggtgtt tttttatcac ttctgctgct gctttaatat tatctctagg aatttttaat
    27001 gctcgagcat agtcgttcgt tgtaccagct gggatgatgg ccattttagg acggtgttca
    27061 agcggagcga ttccattaac cacttcgttg attgtgccat cgccaccagc tgccactact
    27121 agatcaaaac ctagttctgc gactctacat gcttcattct tggctgaatc tggttctggt
    27181 gtagttgcat aggcgctggc ttcaaatcct gcttcttcta atgcctgtag gatatccgcc
    27241 aaattttttt tcaataattc ttttcccgag gtggggttat aaattacgcg tgcttttttc
    27301 attttcagcc tctatcgttt cgctaattca tctttaagta gcttattcac gacaccagga
    27361 tttgcttgtc ctttagttgc tttcatgatt tgaccgacta ggaagccaac cgcacgatct
    27421 tttccatttt tgaaatcatc gacagattgt tggttgttat ccaaaatttc attaatgatt
    27481 ggcagtagtt tgttcggatc agataattgc acccaaccat ttttttcaac tacttcttgg
    27541 gcttctccgc catttgtgat caattcacgg aagacttttt tggcgatttt tgagctgatc
    27601 gttccatcgg cgatcaaacg aatcattcct gctaagttct caggtgtcaa ttttgtctca
    27661 gctaattcca gtttctcact gttcaagtaa gcagaaactt cacccatcaa ccagttagat
    27721 gcttgcttgg catctgcacc attttccaaa acagcttcaa aaaaatcaga catttctttt
    27781 gtaagtgtca ataccattgc gtcatactct ggaagtccaa actcacggat atagcgttcg
    27841 cgacgagaag ctggcatctc aggcaaacta gcttttactt tttgaatcca ttcgtcatcg
    27901 atcacgaatt tcgggatatc aggctctggg aagtaacgat aatcactaga cccttctttg
    27961 acacgcatca aaactgtttt attcgttgtt tcgtcaaaac ggcgtgtttc ttgttggatt
    28021 tctcctcctg aaagaagaac ttttgcttga cgtttttctt caaaagctaa accttttttc
    28081 acgaaagata aagagttcaa gtttttcaat tctgctttcg taccgaactc ttcttgtcca
    28141 taagggcgaa gagaaatatt ggcatcacag cgcatagagc cttcttccat tttcacatcg
    28201 ctgacttctg taaattggat aattgatcgc aatgcttcta gataagcgta tgcttcttct
    28261 ggtgaacgca tatcagcttc agaaacgatt tcgatcaaag gcgtgccttg tcggttcaaa
    28321 tcaacgtaag aataaccgcc aataccatgc atgttttttc cagcatcttc ttctaaatgg
    28381 acacgttcga tccggatttt tttcttttta ccttctactt cgatttcgat ccatccgtca
    28441 tgaccgattg gttgatcaaa ttgggaaatt tgatatgctt ttggattatc tgggtaaaag
    28501 taatttttcc ggtcaaaatg agtatcttta gagatttcac agtttaatgc caaagctgct
    28561 ttcatcccga attccagcgc agctttattc attactggca gcacgcctgg ataaccccag
    28621 tcgatcacgt ttgtattgct gtttggttct gcaccaaaat gagctggcgc tggagagaaa
    28681 atttttgagt tggtttttaa ctcgacgtgg acttctagtc caatgactgt ttcaaaattc
    28741 attcgtcttt ccctcctaaa atagctggtt gttgtttatg gaattctgtt gcttgttcaa
    28801 acgcatatgc cgcttgatac atcgtttttt catcaaaata attaccgatg atctgcaatc
    28861 caactggtaa tccttctgat aaacctgccg gtacagacat acctggaagt ccagccaagt
    28921 tgacagggat cgttaagatg tcactcatgt acattgtgat tggatcgtta atgttctcac
    28981 cgattccgaa tgcaactgtt ggtgaagatg gcccgatgat caagtcataa tctgcaaaga
    29041 ctttttcaaa atctcgtttg atcaatgtac ggacttgtcc tgcttttttg aagtaagcgt
    29101 cataataacc cgcgcttaag gagaatgtac ctaacatgat acggcgtttt acttcttcac
    29161 cgaatccttc tgaacgtgag ttgacatata catcatctaa tgatttcaca ttttcagagc
    29221 gataaccgta gcggatacca tcgaatcgtt gcaaattgga acttgcttct gatgaggcaa
    29281 tgatataata aacagccaca ccgtattttg aatgaggcaa gctgacttct tccacaacag
    29341 cacctaatga acggaatgtt tctgctgctt tcaatacagc ttcttttacg ccaggggcca
    29401 caccttcgcc taagtattct ttaggtaaag caatcttcat tcctttgatg tcgcctgtca
    29461 gtccctctgc aaaatcagga actgaaacgc ctgaagaagt accgtctttt tcgtcataac
    29521 cgctgatagc t
//
LOCUS       DACDXE010000033        28280 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.033, whole genome shotgun sequence.
ACCESSION   DACDXE010000033 DACDXE010000000
VERSION     DACDXE010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.033"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(330..3608)
                     /locus_tag="IWM23_001461"
     CDS             complement(330..3608)
                     /locus_tag="IWM23_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAP9884111.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3879..4424)
                     /locus_tag="IWM23_001462"
     CDS             complement(3879..4424)
                     /locus_tag="IWM23_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9884112.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(4436..5128)
                     /locus_tag="IWM23_001463"
     CDS             complement(4436..5128)
                     /locus_tag="IWM23_001463"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAP9884113.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(5147..5431)
                     /locus_tag="IWM23_001464"
     CDS             complement(5147..5431)
                     /locus_tag="IWM23_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884114.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(5444..6007)
                     /locus_tag="IWM23_001465"
     CDS             complement(5444..6007)
                     /locus_tag="IWM23_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAP9884115.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            6235..6897
                     /locus_tag="IWM23_001466"
     CDS             6235..6897
                     /locus_tag="IWM23_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAP9884116.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            7021..8217
                     /locus_tag="IWM23_001467"
     CDS             7021..8217
                     /locus_tag="IWM23_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAP9884117.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(8318..9694)
                     /locus_tag="IWM23_001468"
     CDS             complement(8318..9694)
                     /locus_tag="IWM23_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9884118.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(9731..10597)
                     /locus_tag="IWM23_001469"
     CDS             complement(9731..10597)
                     /locus_tag="IWM23_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAP9884119.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(10686..11711)
                     /locus_tag="IWM23_001470"
     CDS             complement(10686..11711)
                     /locus_tag="IWM23_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9884120.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(11729..12403)
                     /locus_tag="IWM23_001471"
     CDS             complement(11729..12403)
                     /locus_tag="IWM23_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884121.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(12587..13480)
                     /locus_tag="IWM23_001472"
     CDS             complement(12587..13480)
                     /locus_tag="IWM23_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9884122.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(13554..15251)
                     /locus_tag="IWM23_001473"
     CDS             complement(13554..15251)
                     /locus_tag="IWM23_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAP9884123.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(15244..16557)
                     /locus_tag="IWM23_001474"
     CDS             complement(15244..16557)
                     /locus_tag="IWM23_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9884124.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(16557..20594)
                     /locus_tag="IWM23_001475"
     CDS             complement(16557..20594)
                     /locus_tag="IWM23_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAP9884125.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTAINRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(20607..21065)
                     /locus_tag="IWM23_001476"
     CDS             complement(20607..21065)
                     /locus_tag="IWM23_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9884126.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(21363..21881)
                     /locus_tag="IWM23_001477"
     CDS             complement(21363..21881)
                     /locus_tag="IWM23_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9884127.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(21883..22500)
                     /locus_tag="IWM23_001478"
     CDS             complement(21883..22500)
                     /locus_tag="IWM23_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAP9884128.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKFGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(22497..23351)
                     /locus_tag="IWM23_001479"
     CDS             complement(22497..23351)
                     /locus_tag="IWM23_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884129.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(23348..24259)
                     /locus_tag="IWM23_001480"
     CDS             complement(23348..24259)
                     /locus_tag="IWM23_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884130.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(24300..25634)
                     /locus_tag="IWM23_001481"
     CDS             complement(24300..25634)
                     /locus_tag="IWM23_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9884131.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            25842..26672
                     /locus_tag="IWM23_001482"
     CDS             25842..26672
                     /locus_tag="IWM23_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAP9884132.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(26916..27165)
                     /locus_tag="IWM23_001483"
                     /pseudo
     CDS             complement(26916..27165)
                     /locus_tag="IWM23_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27356..27550)
                     /locus_tag="IWM23_001484"
     CDS             complement(27356..27550)
                     /locus_tag="IWM23_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884133.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(28055..28240)
                     /locus_tag="IWM23_001485"
     CDS             complement(28055..28240)
                     /locus_tag="IWM23_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884134.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
BASE COUNT         8205 a         6124 c         4916 g         9035 t
ORIGIN      
        1 accgagaaac tggcttttga acaccgttta tcgagaggtt gtgaaatcag cgtcctgacc
       61 tgagtattag agaaaaaaac ggaaaaatag ttcctcatat tttgccgttt gttgagctaa
      121 taccgagggt cagctgattg aacaccgttt agtggagaga ttgtgaaaaa gctgtcctgc
      181 atcaagtaat aagaacagcc aatcaaaagc agcaaggaga acctcatcta taaaaagata
      241 gaaggaagtg agacatctat tggtctcatt tccttctatt tttactttcc tcagtcaatt
      301 gttttacatc ataaaaggag aaattccact tatttttttc gctgtttcat gacataaaca
      361 actacaccga ttaacaaaat tcctccagct aatgctagca acgggttatt aaattctccc
      421 gtttttggga gtccttgagt gccatttttt tcactttctt ttgctgtcct atccggatga
      481 gaaggttttc catttggttt tccatttggt gattcatttc ctggattttc tctttctggt
      541 ttgtcatttc ccgggggcac atcggcagtt tctcgcacag tgatcgcttt ttgctgggta
      601 acagattctg tatgagcaga ccatttttcc ccatcaaata tttccgaacg ataagtgact
      661 tctagcgtat attctccttt tgaaggtgct gaaaaaatag cggtatgatt agaaaattga
      721 gcacttgact gtgtttcaac gatttggatc ttttctggga aataacgcat atcttcaaag
      781 acaggcgttc cttgatttgg tgtgaaagtt gacacaaaaa ctgtaaaagg cttcagttca
      841 atgatatttc cttctgcatt ctccgccaca ccatttgctt cttgtataat aatcggatta
      901 ttttgttcat ttggttttgt ctgatttggt tcttgtacga ttgcatactc tggatattcc
      961 gaagacggtg aggtttctcc gtcaaaaata gcacctacag ataatggaag accatccact
     1021 ctttcctttt ctgtttgacg attccccggt ttaatctgca tcagtccatg aacatggata
     1081 cttccatttt catagcgcgt aacaggctgc tgaccttctt tcgaacatgt atttagagac
     1141 tcgatccaat cttttgatat ggtatgtcca agagtatttt ctgattgttt ctctcgttca
     1201 ttccaatagt agttcaacga gtggtagatt tgatcttcgt tttgattcaa tgggcggatc
     1261 tgctctctca tctcaagatt ctctgttcgg aaagataaga taccagaagc atggtagtct
     1321 gtgtttcctg ctgaagaggt ataaagagcc acattatatg aaccgttatt aaaacttgta
     1381 ctgctattga cgataatatc tgttccgcta ttggaatcga ttcctttcgc aagattttcg
     1441 aaagacaaac tattgatcaa ttcatgaggg cccgttaaag aagaaccgcc cattttgaat
     1501 ccattaccgt taccttctcc ctcaattcct ggtacccagc cgtttcgata agcaactgag
     1561 tttttgatga ttacttttcc gatcgaacct gtttcatttt tagcaaaaag gtcccaaccg
     1621 tcatctgcat tatgatacgc gatacaaccg tcaaatacat ttccttctcc tattgtcagt
     1681 ttggccgcaa atccatcggc atcttcaaat cctggatcgg cattttcaaa agatgtacag
     1741 ttcttgacta agttatgcgc gggccatgct tcgaaaggat catttgagct accgctgatt
     1801 tggattcctg tatttccatt ccgataagca tgaatctctt cgatcaaatt atgatttcca
     1861 gaaatctgta atcctttttg catagctgca ctatttgtaa tgtcaaaccc tcgtaaaccc
     1921 cagtagtgac tgaacagtgt catcccattt ccagttcctt taaaatcaag tacaggacgc
     1981 ttgttttctg gatttttctc tgcaatcaac tgaatcgggt tttctcttgt cccgttaacg
     2041 ccttttggaa tcgtcaatcc tctttcgaag gaataagttc caggcgccat gacgattgtc
     2101 tgcccagcat atgcgtatct caatgcttgt gtcagagata atggattttg tctgcttccg
     2161 tttcctgcat ctgacccttc tggtgttaca taaactgtcg aatatttagg tttacggtag
     2221 tccactgtat gaagaatctc ttttgtttca taactagtca tctctacgta ttcttctggc
     2281 ggatagtttt tatctggcgt aaaactgaaa gtaaattcgt tcgttccttc catcagtttt
     2341 gtctgataat caaattcccg attcgcagtg atagccaagt tttttgcgtt cttaagcacc
     2401 tctcctgttg ctttattttt aattgtcaat aatccgtcag catttgctct aaaggacata
     2461 gtatgatctt ggtttcctga atgattacta gaggtcacag tgtaaatcgg atcaatcaag
     2521 cgagtaggtc tttcttgccc aggaggatct gtcgcaggat ctgagtaagc aacttcgatt
     2581 ccttctacct tgatcgtcat attccttgct gcagcaaatc ctgcataaat aaccgattca
     2641 tctgaagcgt ataatttttc ccagtcatac atgatgtcag aagccatttc ttctcctgtt
     2701 tcaggatcgt aataatatgc ctctaaacca gtattcgttt ttttcatctc tacgtccagt
     2761 ttagtaatcg agttcgtggc cggaaacaga ttcccatgac cgtttccgaa tatattataa
     2821 gaacctgttt ctttttccaa gaaccctgct gaagtctcta aaggtgttgt ttccactgag
     2881 acactcccag gagctggcgc agcttgcgga tcttcagatg atattcctgt gattgcccgt
     2941 gtccccaatc caagtcgcat cgtatatttg ctgccgctcg tgttgaccac ttcttgtcta
     3001 tccgagtccc agacatattc gatccgactg cctagtatgg caaatgaatt tgaataaaat
     3061 gcctgcccaa acaaatgatt ttttggtact gcgtctctta ccattaaagc aaatccttct
     3121 tgggcattcg tataggtcca agattctatt tcgatcgtcg cccgcatcct aaagttgatg
     3181 tctttgggga ttgcttgata ataataggtc attccatctg atccgcttga ctggaatttt
     3241 ccgccgttat tcgtggaaga gagccgcacg ccgtcttcta atggttcaat ggcataccta
     3301 ctagcacttg tgctttctcc gaaaacaacc cctttccact cttcatttgc ttcttcagat
     3361 tgcttttttg tcttttcatt ttctgattct ttcatcatag gatcatttcc ttcttccgta
     3421 tccatatctt cactagttct tggtatttcc tccattgttg acatcccttc aatgttcacg
     3481 tgtgcatttt cttcgttcac agactgttgt tcgttagcag caacttcaac gacagaaatt
     3541 aaaaccgggg tactgagcat aaggagattc atcattagcg cactgtattt tatacgtttt
     3601 tttaacaagt gtattcctcc cgtaacaaat tagaaagcgt ttacttaaag tattacctaa
     3661 tttattttta ctgtcaataa aaaacaaaat tataataagt gtattttata aaaagaaaaa
     3721 atataactta agttgttgat aataaagaga tttattaacg gaaaaagaat aagttttttt
     3781 aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg ttttatgctc ttcttttatt
     3841 attttgtgct ttttatttta tcaaatcact ttgttttttt ataaaatttc tgcgcctagt
     3901 gtggactcaa aatgtctcaa tgcccattca tgtccaacgg gatcgaaact tgccacggaa
     3961 tctttgaaaa tatggagttt gtatcctaga ttatacgcat cgactgctgt atgcaacaca
     4021 cagatatctg tacatacgcc ggtcaaataa atatctgtga tctggcgttc tcgcaaacgg
     4081 atgtctagat ctgttccact aaaagcggaa taatgccgtt tgtctatcca ataaacattt
     4141 tcctcttgct catagtcttg gtataaactt tttaaagagc cgtacaattc ccgtccaccc
     4201 gttcctatca cattatgcgg aggaaataag cggttctccg gatgaaagga atcttttgga
     4261 tcatgcgcat caatggcaaa aacgacaaaa tccttttgat caataaactt ctttgtatga
     4321 gcagtcagct ttttttcaat tacttgtcct gctgtcccag tagtcagctt tccatcctct
     4381 gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt tcatcttagt cctccttata
     4441 cgagatgttt gacaaattcg atcaccattt ctgctgatcg tttgcctgcg tcttcgataa
     4501 attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc gctcattgca cgtacgatca
     4561 aaaatgggat attgaactga cgagctgttt gtccaacagc ggctccttcc atttcgcaag
     4621 ctaatgcttc agggaaattc gttaagattt ctttgatttt atctggcgag tcgacaaatg
     4681 tatcacctgt aacgatcaat ccttcttttg cgttcaaatt cgttgctttt gctgctttaa
     4741 ccatttcact gcgcaaatat gtgcttgctt cataatatag aggcattcct ggtaattgtc
     4801 ctgggttgta gccaaatcct gttgcatctg catcaaaata agctactttg tctgatataa
     4861 cgatgtcacc tacttgtaag ccttcaccga ttccgccagc tgaacctgta ttgatcacca
     4921 tgttcacgcc gtattgttgg atcaacagac ttgtggtaat agatgctaag acttttccta
     4981 taccggaacg tacgacgatc acttcgtggt tacctaatga acctgaaata aataaagcgc
     5041 ctgcgcgttc ccatgataat ggttcgctta ggttctctct caaaattttt acttcttctt
     5101 ccattgcgcc gataataccg attttcatct ttttaattcc ttctttctat ataaatgcga
     5161 ttgctaaaaa aaccacacat aacattagga taacaataat aagagatttc atcaaaaaag
     5221 agttccattt ggtcgtcttt tcccgttcgc tgatccttgt tttcgtgatt ggtttattct
     5281 ttttgctttc tttacgataa aagctggcaa tctttttttc ttcttgctga taagctgttt
     5341 ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc ttgttgtcgc ttgcgaagtt
     5401 cgcttcgcgt aataagcggt cctttggcca taagccgatc cctctacttt cctttggtca
     5461 atagttccca gtattgaatc gcataaatcg tctttgcatc acaaatcaac tgatctttca
     5521 tcgcttgttg cgcctcttca agagttagat gatacaactc cagtacttcg tcttcatctt
     5581 gtgctaaagg attctcgacc ttctctaccc cttgtgcatg ataaatatga aggacttcat
     5641 ttgcaaaacc tggtgaaaga tacatcgaag taagatggct cagcgacttc gcacgatagc
     5701 cagtttcttc ttctaattct ctagctgctg tcatttcggg attttgtcct tctcctggat
     5761 cgatttttcc agctggaatc tccaaaatca ctttttctaa aggttttcga aattgtttga
     5821 caagaagcag acgatcttgt tcgtcaaaag caatgatccc gacaccgcca ggatgaaata
     5881 ccagttctct cttcgaagtt cctccatctg gcaaacgaac ttcgtctaca gcaacatcaa
     5941 taatttttcc gctatagatt tctttgcgac tgattgtttt ttcttcaaat tgctcattgt
     6001 tcagcatatc ttctcctgct ttctttgtaa tcataactgc ttttttttca tttttcaatt
     6061 tttctcaaaa actccttatt agtttaccat agataaacct tagattttct ctaaatatag
     6121 aaaatccttc tttagtccga tactctttct agtagtccgt atgctaaaat aagtatgtaa
     6181 tcgaataacg aaaagaattc aatactcaac gataaatgac atggagggtt tattatgata
     6241 aaacgaaatt ggccgtggct tctagcattg gtcttgctat ttctctttgt ccgaaacaat
     6301 agtttttctt ttgttctagg acttttattg ataggtgtag ttttagtttt gtggggactc
     6361 tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc ctgcactgac tgacgaactt
     6421 gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa tttcattttt tcgacaaaca
     6481 atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga atatgcagca gacagccaaa
     6541 cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag cgaaagcttt attcaaagaa
     6601 ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt ttctttatac gcatctgccg
     6661 aatctagttg atttgacaaa taagtataat gagatcaatg accatgagat caaaaacaaa
     6721 cagacctatg aaaaactaga agaaagcgcg cagatcatcg accagctgtc agctttaatc
     6781 gcacaagatt atcaaaaatt cgttgctgag gatctagatg atatcgacgt agagatatct
     6841 gttgcgaaac agagcttaaa gcgtgataat aaatatgaac cagaacaaaa ggattaactc
     6901 ttttgttcat aaaagaagac tcggaacatt cagatgatgt ctggctgttt cgagtacttt
     6961 ttctaaagta gagacattca tgtaagaatc gagaacagat tcaacaagga ggaatttttt
     7021 atggatcata aaccgactga aacaaaagat gttacaccag ttaacgatac acttgaagat
     7081 ttattgaaca acccgttttc aactccaaca gatacgctga ccacttcaca acaagcggag
     7141 attgatcagc tccaagagca acagacagct gctcgtttaa ttgacaaact accggcggaa
     7201 cgccaagaac aagctaaaca gctagcagca aaaatcgatg ctaatgatgc acagtctgtc
     7261 atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat tctcacaatc gatgttgaat
     7321 catgtgcaag cacaagatat tggtcctgta ggagattcct taactgaact gatgtatcgt
     7381 ctccaagaag ccaatcctga cgaactgcgt gccggagaag ggaacatctt ccagcgtgtc
     7441 ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa aatatcaaaa gattggtgca
     7501 caaattgaca agatttcagt gaaattagat aaagaaaaag acggtctgct aaaagataat
     7561 ctgatgttag aacagcttta ccagaaaaac aaagactact ttgatgcact gaatatttat
     7621 atcgctgctg gagaattgaa aatggaagaa cttcaaacaa cgatcattcc agaagcgatg
     7681 aaaagagcag aagaaactgg cgatcagatg gatgttcaga ttgccaatga ttacacgcaa
     7741 ttcctagatc gtctggataa acgtacgcat gatcttcgct tagctagaca gatcacgatc
     7801 cagcaagcac cacaaatccg tttgatccaa aacacaaatc aggccttggc tgaaaagatc
     7861 caagcatcta tcgctacagc cattccgctg tggaaaaacc aagtcgtgat cgcattgacc
     7921 ttgcttcgtc aaaaagatgc agtaactgct caacgccaag tctctgaaac aacaaacgac
     7981 ttattgaaga aaaattcaga aatgctgaaa atctcagcca tcgaaacagc gaaagaaaat
     8041 gaacgtggaa tcgtggatat cgagaccctt caaaccactc aaaatgattt gatcgaaaca
     8101 atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa aacgtcgtca tgcagaagta
     8161 gagcttggac acatggaaga agatctgaaa caaaaactgt tggatttaac ccaatagatc
     8221 acctttcgct tgttactaaa acaaacgccc aacaacagtt tattacaact gttgatgggc
     8281 gtttttctta tctttcttct aacttctcca ttggagtcta tttctcattc atcttgctgt
     8341 gtccatagcc atagccaaaa taaatgatca gtccaataac cattacgata ccgaacacga
     8401 tccatgtcac agtttgcaga cgaagcatca atacaataca agtcaagatg gagacgattg
     8461 gcaatagcgg aacgaatggc actttgaatt ctccagcttt tggtttgcca aacatttttc
     8521 tcaacttcag taagcctaga ctaacaagca tcaaataggc gagcgccatg atattcgtta
     8581 gttccgctaa tacattcaat gggaaaaatc cggcaaagaa tacagctgcc acaccagcgg
     8641 cataagtcgc ttttttcggt gtacgatgct tttgatcgat ttctgtcata aacttaggaa
     8701 gtaagccatc cttgctgaca gcaaacagta aacgagcaag tgaatacatc atcgagatcg
     8761 ttaccgtcag tagcgttaaa attgccccta cagcgatcac cgcgcccact gttccatgtc
     8821 ctacatacct cattgcaaaa gccactgggt cattgactcc tagttcagta tacggaacga
     8881 ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt agcaatcagt atcgaaccaa
     8941 tgattcctct tggcacatct ttttgcgggt ttttgacttc ctcggctgcc attgcaacgg
     9001 catcaaatcc caagaaagca aaaaacacaa gagctgcacc atctagcaca ccggaaaaac
     9061 caaaaggcat gaatggctgc cagttatcag gtttaacgaa aaatattcct acaatgataa
     9121 acaacgcgat gataccaaat ttgacatata ccatggcatt attcaaacgt aatgctttct
     9181 tggcttcaag cgacacgatg taagtgacga atatcactac gagcaccgca atcaagtcaa
     9241 tgtatgtacc attttccgga ttgtacccgc cagttaatgc ttgtggaagt tcggccccta
     9301 aacttttcaa aaaaccttgc atatagccag accatccgga agcaacagag gaaaccgcta
     9361 gtaaaaattc cccgatcaac aaccaccctg tcagccaggc agcaaattca ccaaatacaa
     9421 catacagata ggcatatggc ccaccaatca ctggcactcg cgaagcaaat tctgcgaaac
     9481 ttagtccaga caaaatgaca acgattgcag ctaagacaaa tgaaagtgac agtgctggac
     9541 cagcattttg attggccgca acccctgtca ccacaaatat tcccgtaccg atgatcgcac
     9601 caattccaag catgatcaaa tcagacgttt ttagttcttt tttcatctca ctcgtatgat
     9661 tgagattgat ctctttctta cgaaacaaat ccattgaagt tcctccccat tttaggttgg
     9721 aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat accacctaaa atgactaaaa
     9781 ggcaaccaac aataacataa atgagctctt ttttcgtttt tctttcgcca aggatgaaaa
     9841 tcccacctaa agtagagata atgatcccca tttgtgaaag tgagaagcca acagctaaac
     9901 caagtgattt gactgttaga agcatgcaga tgtttcctaa gccccataat aatccacaga
     9961 tcatgttttt ccacacaaac tgatcgactt taactttacg gaaagcaaag aagcttgcgc
    10021 ctaagatcat tccgatactt tgaggtaaga taattgacat cgcatccaaa ttcgcccagt
    10081 tgacgatgat ggtatataca ccataaccaa tcgtagagaa gatcaatgct ctaaaaccct
    10141 taccaaaatc aagcatttta ttatctgtat ctatttttcc ttcgtcatct tgtcgggcag
    10201 tcaaatacgc accgcttaca agaagagcta aagcaataat accgtataca taatctttcg
    10261 tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt attcaaaatt aattgaaaac
    10321 cagtagacaa tggtagccct acagaaacac ccatgtactt cattccatgg aactgtccat
    10381 tttgtccaac actccaagct aagccggata aaaatccgat caagaaaatc caaggggtga
    10441 ttacaggact aacaacgaaa aagacaacta aagaaaacag aaatgcaccg atcgtcattc
    10501 ctagtgtttg ttgattggca tcgccaccaa ttttaccact tactaaacca atactacccc
    10561 atgccaacat agggactaat gctattaaaa tttccataac caatctcctt ttatttcaca
    10621 tgataagaaa cttatcagcg aaaacgttaa caacacaagc actttttttc gtttctaacg
    10681 attttctaac gtttttcatt ataaaaattt aagattggga aaaataaagt cattgttgtc
    10741 ccttttcctt cagttgattc agccgtcaaa tggataccta atttttcggc cagatttttt
    10801 gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa gtcgaccatt tctcccagta
    10861 aatcccttat caaagatccg tttcaagtct tccgctggga taccgatccc tgaatcttta
    10921 actgaaaggc tgactccttg cgcttccttt tctatgctca caatgatgtt tccgtgatcc
    10981 ggcgtatatt tgatggcatt actaagcaat tggttgaaga taaaagcgat ccatttagca
    11041 tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc gaatattttt ttggataaaa
    11101 taatttgcct gactgcgtat cacaggctga atgattcctt ttagtccata ttcttggatg
    11161 aggtagtccc gcgagaaact gtcgagtctt gcataataca aaacttgttc tacgtattca
    11221 tcgatttttg ataattcatt ttctagtaat gtatactttt gatcgtcaat atcgaactcg
    11281 atcgaacgaa ctagcaattt agaagcggaa agtggcactt tgatttcatg gacccatgag
    11341 tcaatataat ctttttggtc ttgttgatta ctgattgctg attgcattac ttcttggtgt
    11401 tcacggatca gctgattgat atattcttct accagttttt cttcttctgt cctcccacca
    11461 ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg ctaattttcc ccaccatctt
    11521 ctcttgctca agtaaccacc cagcagataa aaaatcagaa agacgccctc aatcaaagct
    11581 aaataagcaa ttgtgctcaa atcaactttg atgtttggcg ccagccacat cacaaagatc
    11641 gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc actgatcttt caaatattta
    11701 aaaaaattca tcttaaccgc tcatcctttc agggaacgat atatccctgt cctacttttg
    11761 tttcgatata tccttggata cctgcctgtt cgatttttcg gcgtaaccga ttgatgttga
    11821 ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca cagtgctcgc agcaattttt
    11881 cccgacttaa gatctttcca ttgtgcttca tcaaaataaa taatagacga tattcatttt
    11941 tgctcaaatc aatgatctct ccgttgattt ccatacttcc gttgtcaaca ttcaacgtaa
    12001 tgccattgtg gctcatcatt tcactagata attctgtgta gttgtaagaa cggcgaagta
    12061 atgcattgat ttttgcaatc agtacatcga tttggaaatg ttttgtcaca aaatcatctg
    12121 cccccatgtt catagccatg atcatatcca tattcgtatt cctgctggag ataaaaatga
    12181 tcggtacttt cgatacttca cgtatttttt gacaccaata atagccatcc aaaacgggaa
    12241 gattaatatc tagtaataca agctgtggtt cttctctttc aaagtctgag aacacttgat
    12301 tgaaatcggt cgttccaaag gtttcaaatt gccatttctc aagttcctcc atgatcaatt
    12361 gtcgaatgat ctcttcgtct tcgactacca tgatttttgc cataaaaaat ccctccatct
    12421 ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa caagttggct tacctggaaa
    12481 atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc gatttaatcg cgtttgtctc
    12541 ttccaagcag cttaaattta ttatgttatt cttaataatt ggtctattat ctgttatttt
    12601 tctttcgttg tttttgtctt ttaaattttc gataacacca ataaccgaaa agcgttagca
    12661 ctagcaatat acctagaagg agtataagta atcgataaag atggtatttt tttgtctgtt
    12721 ctatctttga agagacttct tctgtaaagg gaactcgtct tcctgtcact agtaatcggt
    12781 gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac tagatcctgc ttttctctaa
    12841 ttgctaatgt atttgttttt gttggaagga caacaatttt ctcaacgact tcataagcga
    12901 gcatccgacc atacacttca agatagaatc ggtcatcttg ctccatttct tcaagatccg
    12961 taaagagttt tttctcagca ataccgctat gggcggtaat cacggaatgt gtactctctc
    13021 ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc tctatctaac aaaagatcat
    13081 tcgtttcatc aaataaaggc aagctaacat tgattttcgg aatatagatg gctccgatca
    13141 tatgttgttc gtaatattct tttcctggat tttggacatt tctaaccgct tgctcaaaag
    13201 gatctttgac ttgtcccatt cctgggatta ctgatgtatg agcaagcgtc tgatttttct
    13261 tttcatgcac tgctaattgt tgctcatgtt tttcttcttt atcgcgttca agctgctttt
    13321 gataattctc gatccttctt tgatccaaat aattgctgag agcatctgct aaaaatggat
    13381 atgtaaaaac tgcagctccg gcaagaaaca tcactgccat aaataattgt aataaaagac
    13441 tgatttgggt ttgaggtcta ttacctattt tctttttcat tttcttcacc ttcttattta
    13501 aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc ttatttttgt tgcttatact
    13561 tcagacttca ttttagattt acggtaccag ctgtacgctc caatcatcaa acttattccg
    13621 attagtaaga atgccaagag cccgttccca ccagtagcag gaagtgaccc gcgtttcata
    13681 ttcggtactc tttctggccc tggattttct actcctacga tctgaatttc ttcattatag
    13741 cttccataag agatgataaa ttcagtaaag gctgcctcac ctgttggaag tacataacct
    13801 tctggcgcca tggtctcttt catttgatag gttccataat ctaacccttg aacctcaaag
    13861 tatccatttc catctgcgac aaattctgtt gcttgtccgc ttgttgccca ctcaattcta
    13921 tctgctcctg attcataaac agctaaaggc gactcccctt tataaaatat tgcgtattca
    13981 gaacggtctt cattccacaa ttcgaatctt gcatctttaa gcaattcact gcttgatgca
    14041 tcatattttt caaactttct tccacctgtt gtgactttct ctggcggctc tactccttct
    14101 tcataatcaa attcatcatt tcgtccgatt tcaataaccg ctgtgttgtt atggaaatca
    14161 tccgcttcta gttctgtgga aaccttcaaa ttatacgtga tcgttaaatc tcctccacgt
    14221 gcatctgcta atctacctaa agtatctact ttggctactc cattttcttc tgtcaaaatc
    14281 gtcacgacaa acccgttgct tactacttct actatataat cttgtccttt tgttaaaatc
    14341 gtctcgccgg ctcgcacttc tatttcctga tcataataag ttagaccaac tgtaggcgta
    14401 tctgtgattt tgtaattttt gataacagcg cttccgtttt caaggacaga atcgatgaaa
    14461 taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc ctctttctag atcaatataa
    14521 gaaatcgttt cttgttctcc agcttcatta ataatcgtta cttgtctaag gtcgctttcc
    14581 tctacattca tctgtttatc tgcttctctg atttcgtttt tcggatacaa atgcacatct
    14641 gtattgtatg tgtctttcca tgtagaacca tcccacacgc catccaccat atcaggcatc
    14701 atcactggca tcataacaac cattggagac gcgattctgg aaatatgagc tggcgaataa
    14761 gtttctaaga aaagatagac tttatctcta gaagaaggtg cttcttccgt cacttggacg
    14821 gtagagaata ccgcttctcc agcttcattc gttgtttgtt tgtcaatact tgtcagtcga
    14881 tattttaaaa accaactttc tgtgtgccgt ttttgaatcc attcaatcgc ttcggcagaa
    14941 ttaagaccgt cttctcgttc cggtgttaaa ggattatctc gaatcaaatc gtaatatact
    15001 tccgttacgt caaacaaatc aaaatcgact cctggaatat tttcttcacc aaaatccgta
    15061 gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg agaaaccttt tttgtgtaat
    15121 gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg ctttgactag ttttccgttc
    15181 ccgaaaatcc ctattcccgc agtaacgagt aacagtacac tcattcccgt gacaatcatt
    15241 tttttattca tttttttctc cccgttcttt ttttattaat aaaacagcac ctaacaataa
    15301 aagaattcct agtaaagaaa tcaacgttct tgcttcattc gtttgcggga atacggacca
    15361 ttttcctgat tgacctggat tgcttggacc actcggctct ttcggtttgt ccgttgtttc
    15421 ttttacggtc tcgttataaa cacgtggtgt tgcggattca taggcagaga ctggaacttt
    15481 cccggattct aattcagcca tctcctgtcc gttaactact acatattgtg gttggccttt
    15541 tgcatccacc caaaattcag ggatgatgac ctcgattctc ttacttgctt catccatctc
    15601 gtaaccttcc acacctttta attcttcaaa ataataggtg ccagaaggca agaaacgttg
    15661 tcctgtcgta acaaggccct ctttatccga aatgaatttg cttacatttt tgtcattcaa
    15721 aggatcattg tctgctggtt tgatccattg atttttcaag tcattagcag gagccatatc
    15781 taaataatat ttcccccctt ctatcatctg atatagagca aaaacagcac cttctaatgg
    15841 ttttcctttt tcagttgttc ctctttgacg tccatacttg aagaagtatg gatctcttag
    15901 ataaccgaca tttttaggat agatatgaat ctcttgcagt tcctcggttg attctgtcgg
    15961 gtgataaatt ggcaaaatag cagcaattgg gatagctgtc tgatcgatat caacatccaa
    16021 tccaacttct ggatcaatcg attcttcgaa aatcaaataa actgcatccc gatcttggtt
    16081 tttcttaggt gctttgatac gtgcaatccc gtctcctaat ccgccaaccg tatctttccc
    16141 agttgttacc gaagtcccta tttggacaag cctttcttct gtgattagct gacggatatt
    16201 gtttctgttc gtttcagcga tttgctggac gaattcttta ggagtcatgt tttcagcagc
    16261 ttttttttca tgataataat cggtcatgtc gtaaatggca aatgtcgctc catttagtgg
    16321 caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc tcagagatag ccaagccatc
    16381 attttcatgg tattcaaatt gatcgttata atcgatatct cgaatcatcc gtttatgcaa
    16441 cacgaattcc acgacatctt cccctgattc ttctgccgac atcgcatgtg gaagaaggac
    16501 tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa cacaacagct ttttcattac
    16561 cttgctccct cctttttaga ttgtctgaca aacaaaccat aggctgctcc tgcaagcgcc
    16621 atgaagaatg cagcaagcag gatatagaat aatcgtccgg atcctcctgt agcaggcaac
    16681 ggattttcag gatcgttccc gacactaagc tgtaatctga ttcgatttgt ctccaaaagt
    16741 tcatattcta actcttgctg tagatcaggc gtctctgctt cttcgtttgg ataatacgaa
    16801 atctcaactt ttagtgtacc cgcttctttt cctggaacta cccgatcatt ttctatgtca
    16861 ggttcttctc gataactaat ttctaaaata aagtaacctg gaagttttct atagctgtca
    16921 ggtgacttca cttccttgat ccgatagacc ccttccaaca agtctgaagc attgaatagg
    16981 gaagaatcgt ttggatctgg tgccaagcct ccctcgatga tttcatactc tcctgcacta
    17041 ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc ggctattcat actgtcaatt
    17101 ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta aggttacttg tagtacacca
    17161 tcgatgattt catagccttc cggcaaggga gcagtttcgt cttcgatgac cgtatcttca
    17221 atgataaact taccttcttc attcagtgca aactggaaca cttgatcaga tagccgatac
    17281 ccatttggtg cgacagattc cgagacctca tattcaccaa tagacagatg cagaggttgg
    17341 tcgtaattta aatcgacata cttttctact cctacggcac gagaatagaa gtatccgatc
    17401 ccaggcaaac gtttcgattc ttctggttct aacggattac cagtgtatgc tcgatacagc
    17461 gggaaggaag aatcccccac tttcctgata tcgaaacctg caccatctaa tgtatggcct
    17521 tcatggtcta attttctaac tcggatctcc cgataatcaa acgtattgta agcagtgaat
    17581 gctacccata aacgatcctc ttcatctgtt gtatacgtga gatattgcca attttcttca
    17641 cctgtttgct tgagttggat agtccctata gcattatcgt aaagtcgcaa gatccatgtg
    17701 tcattttcat caggttcttt gaaaccttct gggttctggc tctcaatgat tttgtattct
    17761 gcatagttgg tagtttcttc actatcctta taaacaaatg aatctggctc tccgattgga
    17821 tgaataactt tctcaccatc cacgattaca tatttcccgt cttcatctac tgaatagaac
    17881 gtgagtctcc ctttctcttc ctctacttcg ttcacaccag aaagcccttt cgctactgga
    17941 tatccgattt ccagtccgtt accatctgtt tcataaagtt caaactctgc gccattgatt
    18001 ggcatattac tgccttcaat tagtttattg atattcatgt ggattggctt gaattcattt
    18061 cgcatttcca gtttgacata caactcatca tactctgtgc cttctatgtt aggctcttgt
    18121 acaacagaaa gcttgtgata ttctccttca taataggaat tcggatcttt accttccggt
    18181 ccgccgactc tcgaaccaga tgtatattca tctacaagct cgtagtgacc taacatctca
    18241 tcttcgttgg ctgcatattt tttgatttgg aattgatacg tctcatctac taactgataa
    18301 cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc tgactgctcc agtttcatct
    18361 cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt ccgtggtagt tttacttgtt
    18421 tcgattggtt cggcttcccc actctctgga ttttggtcat ataatgcaaa ttcgactcca
    18481 gccaacggct cgccattttg acccttcttg ctgacttgga aattacggcc tttatgttcg
    18541 taacgaatgg aagaagatgg gaaatacatc cgtccagtag tgatatcatt ctcataatcc
    18601 tccaggttct gaatataggc catattgttc actgtgcaaa aatttccgtc ttgagctccc
    18661 gcatcaagtt ttacttggta cttgatcctg accatttccc cttcacctaa agaaatcgga
    18721 gaaaatttat aagcttggtt ttcccgtccg acatacgttg tcggagtcgc cactacttct
    18781 ggtggggctt gtccatgacg ttcttcatcc catatttctg gctcttggta cttcatgccg
    18841 ttttctcctg gatctagatg gtaaaactga atatctgccg atctgccagc tccaaatggt
    18901 gagacattaa aaccagtttc atcataaagt gtggcttgct ggatcgttct cttgaaggta
    18961 gaagagatat cctccaaggc tgtaacaatg tcattcttgt tgttcgcatt gtaatatcgc
    19021 ggtgttacgc catcttcttc taaatcagca atatttttca atacatttcg tccaatagca
    19081 tctagtcgtt gtctcccgct aacgtttcca tcgattccta aaccaatgga gaagatactt
    19141 ggcgggttct cttgatactt atttttcagc caatgatgat aagcaactgt gttgatcgca
    19201 gatgactttt cttgcgaagt aggactgcca ttcgtattgt tattagaaga accatcacca
    19261 acccagtgag tcgctttatt atctccttct tttacctctc cataacgata gtgtacattt
    19321 acttcatctt ctggacggtt aaagtcagat gcataggtaa ctggatgagt aaaccctgca
    19381 ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca aaacgtcatt atcttcagca
    19441 atcttatctt ccatcacagt ccagttgctc caagctccat tagtgctcgt acgataactt
    19501 gactgaatcg gtgcataact gaaggtcggt gtcccatctc ccacaacgat taggattttt
    19561 tctggatttc tattttctcc gccattgtct ttatacaatg tttcatagcc atttttaagc
    19621 cctagtgtaa gcggagttcc tccgattggt gtacgattta ttgctgtatt ctcacttccc
    19681 gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg aactgatttg ctcatggtta
    19741 ttgatagctg tactattatg gtaaaaattc accataccga ttcgaatatc cagactcggg
    19801 tcagagagca gatctttact catttcgttt actgcctcaa tcaacgcagc atttttagtt
    19861 tgactattgg tcccagcggt taattcactc atactcgctg acttatctag aacaaaaacg
    19921 atatcgattg gactgatttc ctttttctcg cccccgatga catctagata gacattgtaa
    19981 agggttgttg catcttcagg atgtagatca gatgctgtgc tgcttggcag aacaggttct
    20041 acccattttt tgatgtacgc cccgttataa tccaagtaac tgttttcaaa attccgttca
    20101 tttccccaaa gctgaacggt ttgagctgga acgtttctat cttcttcgcc tgcagttgat
    20161 ccattattag tagcatagtc gtaatttcgc cagttcgctc ccccgtttgt ataatcacta
    20221 tcatcgatca agttctctcc gtcatatggc gagcctttgt ctccttgata gcgttcattg
    20281 ctttgtgtcc aatttcctaa cacttttgta tagtcattcg ttgtaaactt cgggtagcga
    20341 tgctgttcgt tttcaacttc tccgctagga tagatatatt cataggggtc ttgcgtagct
    20401 gttggcccaa gacgctccaa aataccgttg aataactggc gttgaatgcg ctggagtgct
    20461 tgaccgctgg gtacgattgg ttcgtccagc ttttccatct cattttcttc gctacgttga
    20521 atttcagcgg aatcttctgt atcagtttca ttttcttgta attcttggtt atctattctt
    20581 tcatcaacca ccacttgaag aggtagttac ccttcgttca catcggggat cttttctgtt
    20641 tcaaaaatca atgtgccggt ttctgcttct tttgaataaa cttttccgac ataccattct
    20701 tgaccatcga tcgtctcgac ttttaggtct tctccatctt catcatagga ggatagacct
    20761 gttccacgaa cgttttttac ggtaccaatc ccggcggctg ccgcatcgat tgctaaacgt
    20821 attctacctt cattggcact ttcctttttt tcaaaagcca gatgccattc aagggtttct
    20881 tctttttcaa tatacttccc ttcaattttt gcatagtcct catctaagaa tacctcatcc
    20941 tcctttgttt ctgttgactc agcagctaca tccagggtcg tcatatgata gactgactgg
    21001 aaatatggtc caaatagcaa actgacagtt actaactgca aaagcttcaa ttttaaccct
    21061 ttcacatcct tatactcctt tcaaaaatat ttgtcttctt tcacaacaat caacaaaaca
    21121 atgcccatct aattataatt taatttaatt ttaattttaa atcaaatgat atccttttta
    21181 gttaaaaaac accaattata caacatttat tattaaaatt aaattataaa attttataac
    21241 gttctcattt aacaaaactt aagttattaa gacaaatgaa taaacaacta cgctttcctc
    21301 gaaacaaaaa aataaagcac aaatgcgcat ttacgcactc atgctttatt cgtacaatta
    21361 attcagataa cggcagcgca taggatgcca ataacacttc ctaaaatagc tcctcctaaa
    21421 atgtcagtag gataatgtaa aagtaaaaaa atcctcgaat aagcgatcaa acaagcaaat
    21481 aaaaaggcta atattcctgc aactggactt aattcgaaca acgcagctac tgataaaaag
    21541 gctagcgctg tatgtcctga aggaaaagaa gaagtcttcg gtttacttgt gtaaaggaca
    21601 agccgacgat ttttttcata aggtcttttc cgtcttgtcg tttttttgat aaaaagcaaa
    21661 ccaacaaaaa aatagatcaa actgccgata agaatggcta gaccatattc tctagtatca
    21721 ggtatcaaga ctaaaacagc agcaaggagc aaccaaataa aaccaccatt agagatatta
    21781 ctgataaatt tcaaaaaatt gatttttcga ctatccgttt tccgttgctt ggcgaacttg
    21841 ttgataatac tatcatcgat tttagcaatc aaacgcaaca tgttattcct cctttttaag
    21901 aaccttttct tgcgtacgat ggataaaaag caggacttga aaataaaaca agaaactgaa
    21961 acctaataaa gcaaaataga ttggtcctgc aaaccagaat gcgacaagaa ggatcaacgt
    22021 gcctgccaaa tagaaaagcg cgcttttgaa atcattaaaa aacaagaaaa atgcagtaat
    22081 ccaagaagct tctttttgtt ccagttgaag caagattttg taactaaaga gaagcgcgct
    22141 taatagaagc aaaaacaacg tagtatacat caatgtatag ccaattcccc caccccaata
    22201 gcggaaaatc tgataatcga tcaatagaag aagaaaataa acacttgcta aactatcttg
    22261 ctttccataa agagagaagt tctcacgaaa ctttttccag aatagtgaac cgaacttttt
    22321 ctcttcattt tttttgtaat gataaatcat atacagaact gttcgacttg cagttcctaa
    22381 tgtgaaaaca ggcaagacgc acaggaacca tagtagctgt aaatggataa gacgtggaat
    22441 ccattcaatg atttctaaga aataatcatt ggctttttta ccaatcgatc gattcatcat
    22501 ccttttgaac ctcctgtcaa atccatccct tcaatgaaat aacgttgtgc aaaaaagaac
    22561 agcaaaatcg tcgggatcaa gacaagtgtt gctcctgcca tcagataatt ccattgtgtc
    22621 gttgcttggt tctgaaaaac ttgcaagcca atctgtaacg tgtatttttc ttgatcttgg
    22681 atatataaca gaggacctaa gaaatcattc caagctgcgt tgaaggaatt gatcgcaatg
    22741 gtaatcaagg caggtttcgt caatggaagc atcaaatgac tccaaatata caaatgattc
    22801 gctccgtcga tttcagcagc ttcgatcaat tcattattga tcgataagta aaattgacgc
    22861 atcagaaata tattgaacgc attgccgaag aacgatggaa cgatcagggg caaataagta
    22921 cctacccagc cgatcttgga gaataataca tattgaggga tcatcgttac aaaccccggg
    22981 atcatcatcg tagaaagaac caaagcaaac atcagctttt tcccaggaaa atcaagcttc
    23041 gcaaatccat aagcaataaa agaatttgaa aggacattac cgatcactac tagcacagtg
    23101 atgaataatg tatttcttgc atagcgccaa aaaggaaagg cggcaatcgt atccagatag
    23161 ttttcccata cgattttttc tgggaaaaag gtcggaggat aacgaacgat ttcttccatt
    23221 ggtttcaatg aagtaaacac catccaaagc aatggaatga tcaaaatgat tcctattact
    23281 gtcatgagga cataacttag tatttttttt gtttgccgat tcacactggt ctttttgaaa
    23341 taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca agcttcaaat
    23401 tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc atcgcataac
    23461 ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa agcaaagaat
    23521 ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa atttggaaag
    23581 aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat ggcaaagtaa
    23641 tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca tataaagatc
    23701 taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa ctccatattt
    23761 tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct ggtccaggga
    23821 tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc agctgcatcc
    23881 ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct gtacggaata
    23941 cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg aaaatcgtcg
    24001 tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc caaaataaat
    24061 catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc ttggatgtca
    24121 aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc ataaatacca
    24181 agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct tttttctttc
    24241 ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc aaaaatggat
    24301 tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa tccttctttt
    24361 ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt cgtgtaatcc
    24421 ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc cgccatttga
    24481 tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc attggctgtc
    24541 ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc tggagaagaa
    24601 agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg tacttccaag
    24661 acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg gagctgcatc
    24721 acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata attgatattt
    24781 tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga cccaaattcg
    24841 gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg gatccactcc
    24901 aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc aaaccaacta
    24961 gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa tggataaaaa
    25021 ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt ttcttctaat
    25081 tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa taacttttta
    25141 ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc accttcatat
    25201 tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc tacatatttg
    25261 gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat gtcctgtaca
    25321 ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca gatgtcaccc
    25381 catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa atcctcaatg
    25441 atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa atcaatattt
    25501 tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc atcgcttgca
    25561 ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa ccctgtaaac
    25621 aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt tttaatataa
    25681 cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa aaattattaa
    25741 tgataccaat actttatata attcgcgagc gctttctttg aattttttaa tctttcgtag
    25801 cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga caccagaaag
    25861 aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa tatctgcagg
    25921 agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg ttccaaggct
    25981 ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca acagaagaaa
    26041 tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt gcgcctgttg
    26101 aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc tctggaatga
    26161 atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc gtgggcaatc
    26221 catggagcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt agagctaatt
    26281 taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg atcgatgatc
    26341 gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat caaaatctat
    26401 gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc ccaagtactt
    26461 tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc tcagagggag
    26521 accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa ggcgaatatc
    26581 aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca gtaacaccaa
    26641 aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac acccgttttt
    26701 tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc acccttcaaa
    26761 agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa agacgctggc
    26821 tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct cttaaaaggt
    26881 ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact cagcttatct
    26941 aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc ttcattttgt
    27001 cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa tttatatttt
    27061 agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat tataaattat
    27121 ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact ttgtacgggc
    27181 taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt tcaatcgctc
    27241 ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc actgacagaa
    27301 acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat ctattttatt
    27361 tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa gccgtataaa
    27421 aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg ataacactag
    27481 ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat agaactagtg
    27541 ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc acggcattac
    27601 tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc tacaaaccag
    27661 gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta aattccattt
    27721 gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca taaccttgat
    27781 acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag tactacctaa
    27841 cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc ttagtaaagt
    27901 atctgaacta ttaattttta aactatcatt tttatcctca actacatgca catctgttgc
    27961 aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg caatatcttc
    28021 agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata aaataaccaa
    28081 cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata aatgttgaaa
    28141 aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac cctataaaag
    28201 ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac ttccttaaat
    28261 aacttttatt atagaaggta
//
LOCUS       DACDXE010000034        27213 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.034, whole genome shotgun sequence.
ACCESSION   DACDXE010000034 DACDXE010000000
VERSION     DACDXE010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.034"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..986
                     /locus_tag="IWM23_001486"
     CDS             123..986
                     /locus_tag="IWM23_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9884135.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1115..2737)
                     /locus_tag="IWM23_001487"
     CDS             complement(1115..2737)
                     /locus_tag="IWM23_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9884136.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            2986..3507
                     /locus_tag="IWM23_001488"
     CDS             2986..3507
                     /locus_tag="IWM23_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884137.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3693..5423)
                     /gene="spxB"
                     /locus_tag="IWM23_001489"
     CDS             complement(3693..5423)
                     /gene="spxB"
                     /locus_tag="IWM23_001489"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAP9884138.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5588..6406
                     /locus_tag="IWM23_001490"
     CDS             5588..6406
                     /locus_tag="IWM23_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAP9884139.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6509..6706)
                     /locus_tag="IWM23_001491"
     CDS             complement(6509..6706)
                     /locus_tag="IWM23_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884140.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6875..7453
                     /locus_tag="IWM23_001492"
     CDS             6875..7453
                     /locus_tag="IWM23_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9884141.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(8498..9001)
                     /locus_tag="IWM23_001493"
     CDS             complement(8498..9001)
                     /locus_tag="IWM23_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9884142.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9360..10385
                     /locus_tag="IWM23_001494"
     CDS             9360..10385
                     /locus_tag="IWM23_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9884143.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10708..11586
                     /locus_tag="IWM23_001495"
     CDS             10708..11586
                     /locus_tag="IWM23_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884144.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12062..12178
                     /locus_tag="IWM23_001496"
     CDS             12062..12178
                     /locus_tag="IWM23_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAP9884145.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13106..13444
                     /locus_tag="IWM23_001497"
     CDS             13106..13444
                     /locus_tag="IWM23_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884146.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13538..13768
                     /locus_tag="IWM23_001498"
     CDS             13538..13768
                     /locus_tag="IWM23_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884147.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14168..14539
                     /locus_tag="IWM23_001499"
     CDS             14168..14539
                     /locus_tag="IWM23_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9884148.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14813..15505)
                     /locus_tag="IWM23_001500"
     CDS             complement(14813..15505)
                     /locus_tag="IWM23_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9884149.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15508..16293)
                     /locus_tag="IWM23_001501"
     CDS             complement(15508..16293)
                     /locus_tag="IWM23_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9884150.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16530..17930
                     /gene="lacG"
                     /locus_tag="IWM23_001502"
     CDS             16530..17930
                     /gene="lacG"
                     /locus_tag="IWM23_001502"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9884151.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            17955..18272
                     /locus_tag="IWM23_001503"
     CDS             17955..18272
                     /locus_tag="IWM23_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9884152.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18285..19958
                     /locus_tag="IWM23_001504"
     CDS             18285..19958
                     /locus_tag="IWM23_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884153.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            19972..21603
                     /locus_tag="IWM23_001505"
     CDS             19972..21603
                     /locus_tag="IWM23_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAP9884154.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            22475..23665
                     /locus_tag="IWM23_001506"
     CDS             22475..23665
                     /locus_tag="IWM23_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAP9884155.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            23799..25988
                     /locus_tag="IWM23_001507"
     CDS             23799..25988
                     /locus_tag="IWM23_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9884156.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            25985..>27213
                     /locus_tag="IWM23_001508"
     CDS             25985..>27213
                     /locus_tag="IWM23_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9884157.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDIL"
BASE COUNT         8733 a         4482 c         5383 g         8615 t
ORIGIN      
        1 ctgtcacaac ctcttatttt gattagaact tgcgaaaata aaccggttaa agtgtgtcta
       61 gcgcaagaga tttgctataa tagacgataa gggtaaaatg taacgagaag gggttgaaaa
      121 atgtgtatca aattgtaaca gattcttgtt gtgatctgcc ataccatgta ttagcaaaag
      181 aaaaagttga ttttttatca atgaatctcc aattgaatga tcaagagtta gtagatgacc
      241 tcggaaagac attcaattat gaccgctaca tgaatcagct gaaaaatggt gcgatgccta
      301 cgacatcaca aatcaatgtt ggccgatatt tagaattttt ccgttcttat gcggaaaaac
      361 agattcctgt tttatatgtg gctttttcca gtggattaag cggctcttac agtagtgcgc
      421 ttcaagcggt tgaattgctg aaggaagaat atgaagaccc tgagatctat gtttttgata
      481 caaaagctgc aagtattggg gaagggcttt tagtattgga agctgtaaga ttgaaaaaag
      541 aaggtaaaag catcgaagaa accctagagt ggctggaaag taattatttg aaagtccatt
      601 catgggtaac agtagacgat ctgaaatatc tagaacgagg cggtaggatt tccaaagcaa
      661 gtgctactct tggcagtcta ttgagtgtaa agcctattat tacggtggat aaagaaggaa
      721 ggctgacgaa tgttgacaaa gttcgaggaa gaaacaagtc gatccagaca atcgtcaatg
      781 agacggtaaa aacaatcgtt gcaccacttg atcaaatcgt ttatgtggct tacgctgggg
      841 atctagagag cgcggagaag gcaaaacggc ttttggaaga gcagataaag atgaaagatg
      901 tgaagcttta tcccttagga ccaacgatcg ccagtcacac aggctatggc tgtatcgcga
      961 ttttttcgat gggaatcagt cgataaaagc agtaactcat tctgtaaaaa agcccagaaa
     1021 ttgattgtaa caaaaacagc gactcttaaa agatagatga gcatcctatc tcttagaggt
     1081 cgctgttttt ttatttttta agctaaatag gccattatgc ccataactcg ttcaattgtt
     1141 tttgaatatc tgcattttcc aaaaattcat catatgtcgt atcagctcga tcgatcacac
     1201 ctttatccga aacggcaatg atgcggtttg ccaatgtttg gataaattga tggtcatgtg
     1261 acgcaaataa gattgagcca gtaaatgcca tcaagccgtc attcaatgct gtgattgatt
     1321 ctaagtctaa atggttcgtt ggatcatcta atacaagtac gttagctttc gaaagcatca
     1381 tttttgaaag catgcaacgg actttctctc caccagataa aacattcact ggtttcaata
     1441 cttcttcacc agaaaacaac attcttccta agaagcttct taagaaggtg ttatcatctt
     1501 cttctttact tgcatattga cgcaaccagt ctaaaatcgt tagatcagta tcgaattctt
     1561 tcgttgtatc ctttggtaaa taagaattac ttgttgtcac accccaacgt actgttccag
     1621 tatctggcgt aatttcaccc atgataactt tgaatagcgt tgttgttgtt atatcatttt
     1681 ctgcgacgaa agcgaccttg tcttctttgc tcaaattaaa agatatatca tctaaaattt
     1741 tctttccatc aatcgttaca cttacatttt ctacctgcaa taagtcgttt ccaatctcac
     1801 gttcaggttt gaatccaacg aatggataac gtctagaaga aggttggata tcgtctaaag
     1861 taattttttc aagcattttt ttacgtgaag ttgcttgttt tgattttgat gcattcgcac
     1921 taaagcgggc aataaattct tggagttctt tgatttgttc ttcttttttg gcatttgcgt
     1981 ttgcttgaag tttagccgct agttgactag attccaacca aaaatcataa tttcctacat
     2041 aaagtttgat tttaccaaaa tctagatccg ccatatgtgt acatactttg ttcaaaaagt
     2101 gacggtcatg ggatacaacg atcacagtat tttcaaaatt gatcaaaaat tcttccagcc
     2161 aattgatcga ttggatatcc agaccgtttg ttggttcgtc tagcaaaaga acatcaggtt
     2221 taccaaatag tgtttgtgct aataacactt ttaccttttg tccaccagtt aattcactca
     2281 tttgctgatg gtgcaattct tcagggatgt tcagaccttg cagtaaaaca gctgcttctg
     2341 gttcagcttc ccatccatta agctcagcaa attctccttc taattcagct gctcgaatac
     2401 catcttcgtc agtgaaatcc tctttcatgt aaatcgcatc tttttctttc agcacttcat
     2461 acagacgctt gtgtcccatg atgactgttt caagtacagt atagtcttca tagtcgtaat
     2521 ggttctgttt caatacggcc atccgttcat ctggtcccat agtaacggaa cctgtggaag
     2581 gctgcagatc ccctgataac actttaagga acgttgattt acctgcacca tttgcaccaa
     2641 tcaagccata gcagtttccg ggggtaaatt taatattcac atcatcaaat agtttgcggt
     2701 ctgaaaagtg caaactcaca tcatttacag taatcaattt ttttcctcac ttttatcatt
     2761 attagttttg acaagtctta tagaattata taggggaaag gtaaaagatt caaggcaatc
     2821 gaacattctt tgtgtttcac gtgaaacaga atgaaaaaaa taaaaataaa agcggagcga
     2881 aaatagctac tataggattt attagtttgc aacagcataa tttttgacgt tatttataag
     2941 agggagtact ataaaactgt aaaagaaatg aaagaaggat tgaaaatgga tgaaaaaaga
     3001 accatcgact ggggctcatt gattttggga attttatttg tacttgtctc attgttatca
     3061 tttcaagacc ctgtcggaaa tttagttgca attgtagtag tctttgcgat ttttgccttt
     3121 attaaaggga tatttgaact gtttgtacgg aacagattaa aagagctaac aggatataaa
     3181 ggaaagatgc ccttaataat cgggatcatc gatattctag ttggcgtctt tttcttattc
     3241 aatattggtg caggagtcgc agcattgcct tttgtttttg cagtatggtt tattgctgat
     3301 tcagttttag cgttatttac tgctgacttg gcaagaggag taagtgaagg atattattgg
     3361 tttacgatca tcgtcaatat tttaggaatt ttattaggaa ttttcttatt gtttaatcca
     3421 atttcttctg cgttgacatt aagcttttta gttggattct attttatgtt atttggtatc
     3481 acacatatcg tctatgcatt tagataagta aaatcaaaca aataaaaaag ccggcttccg
     3541 attacagttt tactgttgta attggaagcc ggctttttta tcatggacaa atatgcacga
     3601 ggtgattttt aggtgtcgag acaaatttat gcaaaaatgt gagaagctat atacacaaat
     3661 tttatcttaa tactcgaagc atagcgcagt tgtcacaacc tcttttcata taattcattc
     3721 aatactggca aatcatggac ttcatatttt tctttgaatg cttggatttc ttcttcagaa
     3781 tattttcttg gatctaaatg cagatcttct actggcaatg ggcgcttgtt attgatcttc
     3841 acatcgatta ctacaggacg atctgatttt gcagctgcat caaaggcaaa tcgaagttgc
     3901 gagtattctg tgactgtaaa gctatttgcc ccgagcgctt ctccaacttt accgaaatct
     3961 gcatcttcta aaaatacacc aaatttcttt tgttccgtat cttcttgttc tgcttcgatg
     4021 aaaccaaaag aatcattcga aaagacaaca ttaataattg gaagctgata tttgacttgt
     4081 gtaatgatat cttgcatgac catagcaaac ccgccatcac cacttaaggt aaatacttgt
     4141 ttttctgggt agcttagttg tgcggcaatt cctcctggca caccattacc catagttgcg
     4201 aaccatccag atgtcgtatg tttttgttta ccgttcatat tcaataaacg aatagaatgg
     4261 atagtcgtgt taccgacatc tgtgacgaaa atcgcatcgt cttcagcaat acgattgatt
     4321 tctttataga cagcctctgg acgaatcggt tcttcctctc gatcttcaaa agatttcaac
     4381 caatttaccc atttttcttt attttcttga tttgctaggt accagctatc agcagggcga
     4441 gcatccccta actcgacaag tctttgcaaa gctgtgtttg catcaccgag gatcgacaaa
     4501 gatgtttcat ggcgtcgtcc aaattttgac gcatcgatat cgatttggat aaattcagca
     4561 gttggattaa aaaatgcacg tccaaatggg aagtcgctac cgacaaataa gatcagatcg
     4621 gcttctgcca atgcttcatt tccgggttta gtagcgacac gtccagcaaa accaagaaag
     4681 ttttcataat catcaggaat aattcctttt gctagaacgg aagcgacaac aggaactgaa
     4741 aaatgttctg agaattcttt gatttgcgga aatccgtttc gcgttccttg accaacataa
     4801 agaactggtt tttttgcttt ttcaagataa ggcaaagcag ctagcaaatc tttttcttct
     4861 ggtaatatca cacttgtttt gtgagtgtga gcggtggaga aaggttgttc gtcaatttct
     4921 tcaaaaccaa gatcaacagg gatagtcacg atagccacgc ctttatgctc ataggcggct
     4981 ttaatggctt catctactac atgcggaaga ctttgtggtg tcatgactgt acgattgtat
     5041 acgctaacat ctgcgaaaat aggattttca tttagttcct ggaaagagtt atagttcatg
     5101 gatgtagaag caacttgtcc taagaacgct actagaggca catggtccat ctgtgcatca
     5161 tacaaaccat tgatcaaatg tgtcgcacca ggtcctgcag agccgaagac agcgccgact
     5221 ttccccgtta attttgcatc tgcagcggct gcgagtgcgc cgacttcttc atgacgaact
     5281 tgaatgtagt tgatttcggt tttttcttga tatagcgcat ccataattga attgaaagat
     5341 cctccgggaa tgccgtaaat atggtcgata ccccagcttt caagaacttt taccattgcg
     5401 attcctgcat tgatttttga catggtgttt cactccttta gcaatgatga tacataaatt
     5461 ttttgtatct agagtataga actttttatc gaaaatccca actaattcgt cttttcacaa
     5521 aaatgtagtc atgtttttgg gaatatgcta gagtagtaca tagatagatt caagagaggg
     5581 gaatggcatg gttgaatggc tattgagact tgaaccttgg cagcaagcac ttgctgggac
     5641 agcttttact tattttatga cagctcttgg tgccgggttg gtttttttct ttaaagaaat
     5701 caaaaaagaa gtgctaaact tgatgttagg atttgcttct ggtgtcatga ttgctgcaag
     5761 tttttggtct ttgttagatc cggctattac aaaagcagaa gaaaatggag atattgcttg
     5821 gctagttgta agtattgggt ttggtttagg cggtgtgttt ttatatatgg cagataaaac
     5881 actgccccat atgcattttg gaccacaaca tgaagcagaa ggattgccta cccatttaaa
     5941 acggacgatc ttgcttgttt tttctattac attgcataac attcctgaag gtttagccgt
     6001 gggggtagca ttcggtgcag cagcaactgc ggataatccg acagccgcta tattagccgc
     6061 tgtttcggtg gcgctaggta ttggtatcca aaacttccca gaaggagcag ctgtatcaat
     6121 cccgttgcga caagaaggtc tgagtcgtaa aaaggccttt gtttacggac aagcttctgg
     6181 aatcgttgaa ccaattgctg gagtgattgg agcgctacta gtaacgaaag tggagctgct
     6241 gcttccttat gcacttgctt ttgccgcagg agcaatgatt tatgtcgtgg tggaagaatt
     6301 gatccctgaa gcacagcaga cattgtccag caaacgtcat tttgcagttt ttggcgtcat
     6361 gagtggattt atcattatga tgattttgga tgtggcatta ggataaataa aaaaaatata
     6421 tatgatatat caaatagggc cgcgaactta tatcgcgacc ctattttttt agtaagttat
     6481 atcataatga agtgaatggt atcatacatt aataatagtt ttttctatgt gtatttcctt
     6541 tgatccagcg aacaagccag atgataagtg cgattgggat tagaactttg accgcaaacc
     6601 ataaaagccc gcttaggaca ctgacaaaaa gactgatgac gaagctagct gcaattagca
     6661 ctaaaagaac catgaagata tttaagaaca tatttttttg acgcatacaa aaccctcctc
     6721 ttgttcattc tttactatag aaaaagtata acagggattg tttaaaaaca cctccaactg
     6781 aagactgatt tttacttcat ctacttaaat gatcaaacaa ttgctttttc tctggtttat
     6841 catctaaaat agagcggaaa aggaggagaa tatcatgcct aaaaatcgtc ttgaagcatt
     6901 tacagatgct gtaatcgcga ttatcatgac tattttagtt ttagaacttc atgaacctaa
     6961 cggagacacg tgggaggcgc tcgctgcatt aggttaccga ttttttatct atattgtcag
     7021 ttttatcatt ttagcgattt attggaacaa tcatcatcat ttatttcaag ttgtacataa
     7081 gataaatgga cgggtcttat gggcgaacag tttctttgtt tttgcgcttt cgctttttcc
     7141 gtttgcaact tcatggatca gtgagtacat caatagcctg gctccagaaa tgacttatgg
     7201 ttttgttacc ttgaccgcta atattgcgta ttatatcttg gcaaaagaac ttgtcaaagc
     7261 aaataaaggc cacgagaaag cattgaaggc gatgttgaat aattacaaga aatcgtatct
     7321 atcgattggt ttgaatgtgt taggaattgt cttagggtat ttagtagcgc cagtagccgt
     7381 gctgatcgca aacatcgtga ttctagctgc ttggatcatc ccaagtcgta ccatcgaatc
     7441 acaatatcgg taaaatgatc cgtgtttaaa atttaagaga aaaatataga aaaaataaat
     7501 aaacgatcaa atatccgagt gatactagta ttcaaagagg gatgcagatg gatcaaaaaa
     7561 gaattcatgt aggactgatt actagttttg tcacgatgct tatttatttg gatcaaggtc
     7621 atttgacttg gacagtagtt ggtgtcgtag gatttttgac attattcaca gcaataaaaa
     7681 aataaacatc tttatgtaat ctgatgttct atctatttaa gaaaagcatg tttacagcag
     7741 tatgattctg ttgtaaacgt gtttttattt gtggaaaatt taatttttag aaaagtgtat
     7801 cgaaaaatag atagttagtt tttccacagt ggataacttc gtaagagata aaaataaaaa
     7861 attagtcaaa gaagggcgta tagatttctt ttgataggag atatacaaca gaaatgaata
     7921 aatattaacg tgttttcaaa cagtataaaa cggtgtggtt tttggaataa aaaaataaca
     7981 tatctcattt tacgcttttg ttattttata cttaaaaaat aacgacacag aaaataccgt
     8041 gtctcagatt tctctactac ttctaaaaac tactgttttt ttcctttgaa atgccttcaa
     8101 aaagtaggat caggcagaat atactggcag aatcaaggag aagggagcct aaataaggag
     8161 aaaaaccata gaaaatataa agtaattact tataaaacaa gctttttata gaaaaataat
     8221 atacctgagt agggtataaa ttaagacgga attcctttct agattgagat attgactagg
     8281 aagaatgtta agattttaag cctttaagct aaaaaaaaac cctttttgtt aaataagggg
     8341 aatatagcct gtcagtttca aactgcaagt cgtcctgctg caataatata gtagattaga
     8401 gatgaaaata gattttttta aaccaatgac tgaataaaaa gaccgtcata gatacacatt
     8461 ggaatgtgtc tattacggtc tttttttacg aacgatttta ttgaagacgg acagttcggg
     8521 tcaaaaataa aaaagctacg atcagaaata agaataaaat gtatcccaaa ataatggcta
     8581 gacggtctaa cttatttgtc gatagatgtt tgtttagaag ttgataggcc cataaaccta
     8641 tcagtcctcc tgttgtatta gttatcaaat cagtgatgtc cgctacgcca ataccgaata
     8701 ggaattgaat caattctgcc aagaagctta caaggaaaat aatccctaat tttttagaaa
     8761 aagaaagtct cttgaaattg acattcaaaa gcaccccaaa aggaaaaaag acaatcaagt
     8821 tataagtggt ctcacgtaat accgtcgtat tttcaaaaga aaaagtagaa aaagggacga
     8881 gattgacatt gctgtaagaa taagtgagaa attgtggaag attaaatgat agcttgaata
     8941 aaatcaacca taatagaaag aataaataaa tccctaataa aaattttgat acataccgca
     9001 tagacatctc ctccatttaa aaagtatacc atcgaaaacc gctgaaaaat cataaaaaag
     9061 tatgaagttt aaggaccaac gtagttatcg tacgaaaatt agaagctttt ttttgttggg
     9121 aatccggttt ctttaacaaa gctaagatag cgagttgctt ggaaaagttg ctatcttttt
     9181 tctgtcaaaa cgactcggaa tgttcgtttt ttgttctaat ttctccaagt tgtttacttt
     9241 ttgatactaa gtttatgaaa aatcaagaaa tagttgttat actaggtaat gctgacaaac
     9301 gggattggca tttcatcttg gttcagcttt ttttccaatt actaggaagg agggatagat
     9361 tgttcaagac ttattttcgt ttcaataaaa tacaagataa tccttttcgt atcatgaatc
     9421 tgatgctact tatctttatt gtattatggt taggttacat aaatcatgat tttttgattt
     9481 caagtttttc atcattaggg atctttactt ttttctatta tcaaaacata ccgatgaaat
     9541 atttgctgaa acgtatgttt ctgatcggtt gcggactgtt ggttgctttc gttttgggga
     9601 tcttatcgac ttatgtttta tggcttgaac catttgccgt agctttggtt gctttttcca
     9661 gcagattcgt gttgcgatta ttccatattt ctaaaccagg tggcttgttt tttgcgatgc
     9721 tttctgccat gggaacgagc atgcagctgc caatcgctca attaccgatc gtctcacttt
     9781 atttttttat gggtgtcgtt tttgccctga ttgcggcagt gattacaaaa ttactggatt
     9841 cacggcctga gcagacaatt gaaaaagcaa ctttaaaaga aagatttcac gaagagcctt
     9901 tagtgatcat tgattctgtt ttctacagcg ctgccttatt tctttccgtt tatgtcagtc
     9961 acggcttgaa tctgcataat ccttattggt tgaccttgtc atgtgcatcg attttgctag
    10021 cagaaaactt ggatgcgatg aagcatcgac aagtccaata tctgattgga tcgatgggtg
    10081 ggttatgtgt gtcggcattc ttgtcatttg tgccgtttac gcagttgcag accatttttc
    10141 tgatcacttt tctatatgga atcgcacaat ttctagttgc tagaaattat gcagttgcta
    10201 atatttttct taatccgatg gcgttgatgc tatctacact tattcgtgga gcatacttga
    10261 tttctttgat cgaatatcgt tttttaggga tcgtcatagg aagtttcatc ggtttaggtg
    10321 ttgcgtgggt aatgacggtt ggtttgcaac attatttaac ggttgttcgt caaaaattag
    10381 aatagaagga caaatatatg tgccaaaaga ggttatgaca agatttttgt cacaacctct
    10441 aattcaaggt gttcaaaagc cagattctca gcattaagtc aaattcacac aaaaatgtgg
    10501 aaaggaactt tcaaaaaaat tttatattta ttctatcaaa aaattttgtt tatgatggta
    10561 actgaataaa ctttcctgag atggtcaatg ctttccttga aagtcttaca gatattcttt
    10621 cttcatagaa agtttaagtt ttatagttag tgtacaataa aagtatgttt ataccatatg
    10681 gtgtccggat aatcagtcat ttaaacaatg aggttcaaag atatgaaaag gggaaatttg
    10741 atgaagaaaa taagcaatga aatccaaatt ctggcagaaa ctttaactct tcccaaagtc
    10801 gttatagagc aaatgattgt acaagcaaaa aacatccttg atgaggtgtt tgagcaatat
    10861 agtcatgaac taatggatcc taataaagca aaggcgactg caaagaaaat agacaaagtc
    10921 tatgaagagg aaccttttgc agcattagct atttatctat atgcagcttc tcaaagttgg
    10981 aaaaaaattt atcaaccatt gaagattcca agaactatct atttggcaac aatgaatgct
    11041 tttactagat ttattcatga acatttccaa gtaacaggaa gctatcgctt tgaccgtggt
    11101 ttttggattt ggcattacac tagtggcttg atctttcgga ttggtgaatt ggagtacgaa
    11161 atgacatatt ttccgaaagg gtacacggat ctaagattag aaggaaagca gtgcctttct
    11221 cttcatattc cttccgatgc agatttatct cagaataaga taagagatag ttacgaaagt
    11281 gccattcgtt tttttgaact ttattttcct gattaccatt accaagtgat gtacacagat
    11341 acttggttac tatcacccaa tcttactaaa tggttgaaaa aagagtcaaa aatctgtctt
    11401 tttgcagccg attatcgatt attatcagtt gatgaacagg acgatagtgg agtcccttgg
    11461 atctttggac gagtagatgc ccaaatacat gattatccag aaagtacgag tttacagcga
    11521 caagcaaaag agcaattgct tgcaggcgag cacattggct ctggcttagg tattcttact
    11581 tcataagttt agtacttcga aatagtttaa aagagcgtga gacaaaagta aagaacactt
    11641 ttgtctcacg ctctaaacac gtataaacgg cggaagcaga agcaactcct tcggaaataa
    11701 gctgaaattc acaaaaattt gaaaagcaat tttcgtgaat ttcttcttat ttcttggagt
    11761 taaacgcttc tgtcccagcc tcttttttag tgttctatcg aacacacagt acgttcaata
    11821 ggtaaactag ctataacaat agatattaaa gttaacggac aagagggcct tttttaggta
    11881 gctaaaacgt aataatgaaa atttatttcc agagatagga gtcaatcagc tagtctgatc
    11941 gactgtttta acaagttgac tttcatttta acttttgata ccatttaaaa cacaaatcgt
    12001 aattgtttcg atttgtgttt tttagcatga ctacaatcaa agaagaaagt gggtcgtcca
    12061 tatggaaaaa acagatatct caagtgcata ccgccgcttg aaaagcccaa atatcaaaac
    12121 acgaaaacgt gcgctgaaga tcatcaaaga tgtgaaacgg aatagtggga aaaggtaaga
    12181 agaaggaaac ttaattaaaa aaatagccga aaaacgtttt tcggctattt tcaataatcg
    12241 gtttttgtgt aaggtttccg aaagaactgc tgttgatttt ccatttttaa catatgataa
    12301 attggtagaa tctttctttt caaattgata ccattcccta attccagtta tatagttaac
    12361 tacagaacca caaagcacgt acgtttataa aagtattatc tgtagttatt tttcaattcc
    12421 ctttcaacct cttattttga ccagtagtat tgatcacttt accttttaac acctttgttc
    12481 gtttttgaaa catttttagt caagaatgga aacgtaacta cataatcaaa ggttttgcga
    12541 aatatttcat gaaatatttc atgtagaagc gagcaatacg gttgcaaata accattatcg
    12601 tttaataagc agttacgaaa gctcccaata tctctaacgt tcagctcact ccatttcttt
    12661 tcttcaaaaa aatcagtgat tcgtatctct ttctatttgt cgaaaaagtt aactttaaaa
    12721 agtaacgccg aaaaaatatg caaaaaaata aaaaagcagt tactattgct agaataacat
    12781 caaggaataa taagttttgg ttttcagata atagtcgata agttgtgtat tggaatttta
    12841 gatacagtaa taaagtattt ttcatgaaat tttgtgagaa tcactattac aagtatcttt
    12901 aataaaatta atggttaagg aggaacttaa agtagaatgt aaatgaatag ttgtagtcag
    12961 tcgattgtga tactagactc aaagttgcag gaataagttt ttcaatttgt gattggaaga
    13021 taaaaaatga aaaagaaggg ctttttttta tactttgttg attgtagtgt ttattttccc
    13081 tctgtttagc caagtaacat tttctatgac tcaaccagtt tcaagtgagg aacatgataa
    13141 cctttttcgt gctacagaag agtttaagaa atatcaggaa aatattgttc cggagactcc
    13201 attagctagt gagccaatta ctgatattga tggaaaccaa ataggatggt atatacagta
    13261 cgaagttatg gtggtaaata atgactcaat agtaaaaaat acagtaggct tagagctaac
    13321 accgattgaa aaggtgagtt cattgttaac gtatatttat actgatgaaa gtgacgagac
    13381 tgagatcatt ttaacggatt actcaaaaat tgctactgat aaaaagtata tattgtggat
    13441 gtagacacta gtgaaatact ttatgatata gaaattgatg aaaatttact agatgtagca
    13501 caagaaatcg atcatcaaaa gcaggaagct attcgatatg ctggtagaaa agggaaaaga
    13561 aaatatttat tacgtgaatg tagcaaaagt tagagaagat gaaaatgagt ggaaagaatt
    13621 caagagtcgc tactcaatta actcgactcc gacatttaca gtttacagag aaggatcgat
    13681 agaaaaaact gttttttgga ctaaagaaag tggaatgtct ttagctgaag tagaagagtt
    13741 tttggattat gttagtatgc aacagtaagg gagttcttat tatgaaaaat tttgacacat
    13801 attatgttat tgttttttcg atatctttgc tgttaatatt actaacttta atgcagtata
    13861 aagagtatcc agagtatttt attttaactt ttgtatattt agtaagtagc tacacacttc
    13921 tatataaatt cagaagttct tctagagttt ctaaagtttg tttcttaatt agcttattga
    13981 ttctataata tttttgattt atataggaaa ttaaaaatat tatagaatca acactcagag
    14041 aacgggttgg tttttaatag atttaataag tactgataat gaaatttctc ctggttggga
    14101 aaacgaagtt ggttaccctg tagaagaatt ttttcaagat tactttgcgg agtatgccga
    14161 taggataatg agagattacg taacagataa aactcaagaa gtcatcaatc aatcccagtc
    14221 attaaatgaa ttatctgata aggcgaaaga agaagcagtt gccattattt cttcagggat
    14281 tagtgactat attgatatga gtggtgaaat caattatggc gataaagaca aaattgtacg
    14341 catgataaaa gcttttatca aaacttttga tgtgaaggca gatcttaagt ttaatgatac
    14401 ttacctagta ggaaaattaa ttaatatcct tgggacagag aaagatacgg aaacgtttat
    14461 ttcctctttg tctatgttgt ttacagataa gcatttttca actcagcttg gtggagagga
    14521 actagtggaa atcttttaac cattacgccc agcattaata aaagtatatc aaaaaagtac
    14581 tcctgatgat ttttaagatt ggtttgagaa ataatgtgaa aacactattt ttaatgttaa
    14641 ttatagatag agatgaacgc gtcatatggt taaaagaatg aaaaagtcaa agatacactg
    14701 ctatatttca ctaaggatat agcagtgtat ctttcatgtc gtttcactgg cagtttttta
    14761 taaaaggagg tgtgcatttc agttgctttt ctttggtagt tagttgaatc gtttaagaat
    14821 taacggatgt ggcagacagg tgctctgtcc aaaaaccagt aaaatctttg atggttccat
    14881 ctttatttag ctgtgcctgt tcaaatactg ttttgttttt accgaatgga tcaattgcaa
    14941 taattgttcc gatccccgca cgtttggcag caagtaatcc tgagtaagca tcttcgataa
    15001 caagacaatc agccggagct aagctcagtt tgtctgccgc tttaagaaaa atatccggtt
    15061 gcggtttccc agggaaagag ccgtcgtcat agacaatctt gtcccagtca aaccagcggg
    15121 ctaaatcgaa aatatcaaaa taaaaagcaa cattctcttt gacagtagcc gtggcaatcg
    15181 ttaatggaat ctgttggtca gtcagctggt caagagtcgc ttcaagccct tttgtcaact
    15241 gaagctcgtt ttcattttgc aagcaaagca tacgataata gctttctttt tcttctgaca
    15301 atacggcgat ctcagaggcg gtcaaatgct ctgaaatgaa atgtttcaaa atctccgtat
    15361 tcgtccgacc atgaatattt tttaaaatat cctcgtctga taaaccatct tttgtatgat
    15421 catgtatcat atgcagccaa gcagcttcat gcagatgaga gtccaaaaac attgttccgt
    15481 tgaaatcaaa tataatccct ttcatagtta cgctcctata gtggtatttt cggttgaatc
    15541 agcaatttgc agatcgacat attgtgcata gtgctcttga acctcccgcg agatctggcg
    15601 atccgtaatg agcagatcga aatcatgcaa attataaaac acataaaaat catctcgatt
    15661 gaatttttta ttatcaacga gtagatattt cttacgggaa ttatttagag cgatctgctg
    15721 tgcttctcct tcttcggtac tgtaagtaga gatcgcttcg ttgtgaacgc catttgaact
    15781 gataaatgct tttgtaaact ttagtcttct taaattgtcg ttggtgatcg gtccgacaaa
    15841 tgcgccggta tttttccgga aatcaccgcc aatcagtaga atttctggtc catctgcttg
    15901 agcaagaata tcaaaaactg gataattatt tgtaatgatc cgaattttct tgttcagtaa
    15961 atgcgtcgcc aatagttcga ttgttgtacc tggtcctaag aaaatcgttt ctccctcgtg
    16021 aatcaaggag gctgctgtgg cagcaatttc ttttttttct tgaatctgca gagtggcttt
    16081 ttcagtgtga gaaagttctt gatctaaagt gtaggaaaga ctttgagccc ctccatggac
    16141 acgcagcagc ttgcccgatt tttctaattc atctaaatct ctgcgaacgg tcatatcaga
    16201 aacatctaat tgattgatga tttcattaac ggtaaggatt ccttttttat tgaccatttc
    16261 tacgatagta agcaatcttt cttttttcaa cattgtcttc acctctaatt taaggataat
    16321 ctatcatata aacggacaac agtcaatcta attcgaacaa aagtaaacat ttagggtggt
    16381 ttttatgttt gttacaatag attttattgc tgttttctgg tttttatgat tatttgtgtt
    16441 tgtttttgtt gacaaatgtt ttggatgcgt ttacaatgca atcagttaga aagagcgaaa
    16501 actgattata gatttgagga gagatgtaca tgcttaaatt accagaagat tttatttttg
    16561 gcggagccac agcagcctat caggttgaag gagccactaa ggaaggtggc aaaggcgctg
    16621 ttgcttggga tgacttttta gaggaacaag ggcgattcag tcccgatccg gcaagtgatt
    16681 tttatcatca atatgcaaag gacattgaat tgtgtgaacg ttttggtgta aatggtctaa
    16741 gattatccat tgcttggagc cgcatttttc ctgatggagc tgggaaacca aatccagaag
    16801 gaatcgcttt ttatcatcgt gtgttcgaag aatgtaagaa aagaaacgta acgccgtttg
    16861 tgacgttgca tcattttgat acccctaaac gtttgtttga ccatggagat tttttgaatc
    16921 gagaaacaat agaagcattt gtttcctatg ccattttttg ttttcatgaa ttcaaagaag
    16981 tcaaggtgtg gagtaccttt aatgaaatct atccagttgc aaccaatcaa tatttgttag
    17041 gtgttttccc accaggaatc aaatatgatt ttacaaagat cgtcgcctgt cttcataata
    17101 tgatggttgc tcatgcccgt gtagtgaact atttcaaaga aaacgaactt ccgggtgaaa
    17161 ttggtgtagt tcactcatta gagacgaaat atgcggcaac agacgcgccg gaagataagc
    17221 atgctgcctt tttagacgat gcattgtcga tccgtttctt attagatgct acctatttag
    17281 gctattattc aactgaaacc ttgactgctc ttgatgagat atgtgaagca aatcaggcca
    17341 gctatcattt tcctgaagaa gatttcgttg aattgaaaaa agcatccacg cgtaatgact
    17401 acttagggat caatcactac caatgccatt ttgtcaaagc ttacgatgga gaaaatgcaa
    17461 tccatcataa cggtacgggt gaaaaaggga cctctgttta taaagtgaag ggaatcggag
    17521 aacgtattta taaagaagga attcctcgaa ctgattggga ctggctgatt tatccagaag
    17581 gtctatatga cttgttgctg cgaattaaaa gcgattaccc acattacaac aaaatctata
    17641 tcactgagaa cgggatgggg tataaagatc agtttgaaga tggcattatt atggatcagc
    17701 cgagaatcga ctatttgaga gtctatttgg aatccttaag caaggcgata acagccgggg
    17761 taaatgtgaa ggggtatttt ttgtggtcgc ttatggatct tttctcatgg acaaatgggt
    17821 ataacaaaag gtatggcttg ttttatgttg attttgaaac ccagaagcgt tacccaaaag
    17881 aatcagctta ctggtacaag ttggtcagtg aaaccaaaac gattatttaa taagtgataa
    17941 tggaggaaga aaagatgaat aaagaggaat tacaaatgct tggttttgag atcgttgcat
    18001 attcaggcga cgctcgcagt acattattga aattattgaa agaagtaaga caaggaaact
    18061 ttgaacatgt tgattcggcg ttgaaagaag ccgacgaaaa tttaaccctt gcacataatt
    18121 cgcaaacaaa aattttagcg gaagaagcat ctgggaaaga gatggagatg ggattcattt
    18181 ttatccatgg gcaggatcat ttaatgacca ctttattgct gagagatctg attcaagact
    18241 ttattgtact gtacagacag aatcaaggat aggagaagaa cgctatggat ggaatcatta
    18301 aacaaatcga aaaagggaaa ccattttttg aaaaaatctc acgtaatagt tatttaggag
    18361 ccgttcgtga cggattttta acagcgatgc cagctatttt attttcaagc attttcattt
    18421 tgattgctgc ggttcctgag atttttggtt accaatggcc tgaatctgtc tccacatggc
    18481 tatggaaagt ctataattat tcgatgggtg ttgtagggtt actcgtttca gcaaccactg
    18541 ctcgctgctt ggctgattcg atgaatcgtc gcttaccgaa aaacaaagta atcaacacca
    18601 cctctgtgat gctggcctct gtggtaagtt tcctgttgtt aagcgtctct ccgattgaag
    18661 gtggattttc aacggaatat atggggacaa agggactact ggcttccttt gtcgctgcat
    18721 ttttaacagt taatttatat aagttttgtg tgctgaaaga tattaccatt aaaatgccta
    18781 aagaagttcc tggaacgatt tcgcaaacct tccgagatat ttttcctttc tctttttcag
    18841 tatttgcggc cgttttaatt gatttaggca ttcgacaaat cttccaaatc tcatttgcag
    18901 aagcaatcat tacggtgctg cagccgctat ttacagcagc tgacgggtac ttaggaatcg
    18961 ctattatctg gggagcgatg gcgctgtttt ggtttgtcgg tgttcatgga ccgtcgatcg
    19021 tggagccggc aattgcggca attatctatg ccaatgtgga aaccaatctg caattgttca
    19081 attccggtga acaagcttct aaagtactga cggtgggttt agggaatttt gtaggaacta
    19141 tgggaggaac tggggcaaca ttggttgttc cttttatctt cctactcttt gctaagtcta
    19201 agcagttgaa ggcagttggg aaagcttcct ttattccagt aagtttcgct gtcaacgaac
    19261 cattgctatt tgcggcaccc atgattttga acccttattt ttttattcca tttttgttaa
    19321 cacctgttgt aaacgtttgc ctgttcaagt tttttgtcga tgtattgggc atgaacagct
    19381 ttatgtacgt tttgccttgg gcaactccag caccgatcgg tttggttcta ggaacgggca
    19441 tgggtatctt agcttttgtt ctagcagcgg ttctaatcgt cgttgacttc ctgatctatc
    19501 taccattttg caaagcatac gatgctgtct tagtagagaa agaagcacaa aaagcagagg
    19561 tgatcgcaac cgaggaaggt gcttctgccg atgtacgtga ggaagtacct gtggcagcga
    19621 gtcaagttat cagcgatgca aataaagaaa tcaatgtgtt ggtactttgt gctggcgcag
    19681 ggacaagtgc gatgttagct aatgctttaa aaaaaggtgc atctgaatcg aaagcaccga
    19741 tcagtgcaat ggcgggtgct tatggttctc attacgaaat gctgcaggat tttgatatgg
    19801 tggtcttagc accgcaagtc aattcttact atgatgatat caaagaagac accgatcgct
    19861 taggaatcaa gctggttgcg acaaaaggag cagaatatat cggattaacg agagatcctg
    19921 aaaaagctgt tcagtttatt ctgaaccaat ttcaataaag gaggagccat aatgaaaatg
    19981 ggaatttggt tgcgtaaagc tgttttattt gcagctgtgt taggggcttt gggggctgtt
    20041 tcaaaaactg ttgaagcatc tgccagcgaa tttattcgcg gagcagatat ttcgatcctg
    20101 tcagatatgg aaaaatctgg tgcaaaatat tatgaagacg atatttcaaa agatgcctta
    20161 gagattttaa aaaataatgg tgtcaactat gttcgactgc gtttgtggca ggatccttat
    20221 aatagtaatg gagaaagtta tggagcaggg acaaatgatc tggaaacgac gattgacctt
    20281 gcaaaacgag caaaaaatct gggacttaag gtgctgctgg attttcacta cagtgatttt
    20341 tgggttgatc ctgggaaaca gaatcttcct aaagcatggc aaggtctgac ctttgaagaa
    20401 atgaatacgg cattgtacga ctatacaaag aacgttctgt cggaaatgaa gcagctggac
    20461 gtttatcctg atatggttca gatcggcaat gaactgaata gcggaatgct ctggccttat
    20521 ggcaagtctt ggggagaagg cgggggagaa tttgatcgat tggctgcttt tttgaagtct
    20581 ggtatccaag ctgttcgaga cactcaacct aaaacaactc cagtaatgct ccacttagct
    20641 gatggtggcg atacaggagc atttacttgg tggtttgatg aaatcacgag tcgaggtgtg
    20701 tcatttgatt taattggagt gtcgtattat ccgtattggc acggttcaat gaatgaactt
    20761 caaaccaata tgaatgcgat cagccaacgg tatggtaaag aagtgatcgt agttgaaaca
    20821 gcttatgggc atacaacagc gaatgccgat actatgccaa atgcttttgg tgaagccgaa
    20881 gcagctgcgg ggggatatga accaacaccg acaggacaag cagagtattt gctggatctt
    20941 gccgatcgga ttcaggcagt accgaataat cgcggggcgg gcttcttcta ttgggaacct
    21001 ctttggtaca acggaaatgt gagttgggca acacaagcag gtatgaatta tctcagtgtt
    21061 caatccacta tgggaaacga atgggacaac caagcgatgt ttgattttgc tggaaacgcc
    21121 ttgccaagct tgcgtgcgtt taaacaagca ggggcgcaaa taaatctagt taaaaatgca
    21181 agtttcgaaa gcgatggacc aacgaccagt ccttccaatt ggcaaacctg gtttcaacaa
    21241 ggaaccgcta agggtacgat caaaacggaa gcgggcactt tttttgggga ctacaaacta
    21301 actttttggc aggatcaagc atacgaggca tcagcttatc aaaatgttag tggcataagc
    21361 aaaggaactt acacattgag tgcctatgta atgtctgggg gagagtttga tactaatcag
    21421 ttatatatca aaaattatgg tggagcggaa ctaaatactg cggttgttaa aacaagtgaa
    21481 tggacaaaaa ttacaatcaa agacattcca attacaacag gtgtttgtga aattggggtt
    21541 tatagcaagg cttcaggaaa tgcttggacg aacgttgatt ttgtttcatt ggtgaaacaa
    21601 tagactgcgg atcgtttcga ttcaagatcg gaggttccct tcagccacat cgttagcgta
    21661 gaaggcagga atcgaacctg cacctcgcct ttgccaaaaa taaccagaaa cgatttccag
    21721 tcatttttac caaaacgagt aaagcaccag acttcatcta cactttgtat tatgctaaag
    21781 agagttcaac gatttcgtaa gaagcaatga aaaaattgca aaaagttgtg cagataacat
    21841 aaagtttgtt taagaaaggg taaccttaga attccgtcgt gaatatgatt tgagcactta
    21901 gatggataga aaaaagttct cggaaaacag ttcgtttttc gagaacttct ttttatgata
    21961 agaatgaaac aaattaactt atattatagt ctcttttggt agggaaccat cttattttta
    22021 atgaaaatca agcattttag ctattgaaga atgttgattt atagggattc cccctccata
    22081 ttatgttggc tctttattcc cactctgttt tcaacgtaaa gtgtataaaa cagaatgcga
    22141 tactatcaag ggagttaaaa caatatacag aacagtaaaa cactataaat aaaaataaaa
    22201 gtgcaataaa agtgcaattt ttgcacctaa tcatactctg tttatatatg aaaactagat
    22261 tctgaatcca atatttggtt aacgagaggc gttcgaattt gaacgtcttt tatatattac
    22321 tttcaaataa atatgaggta gaatactatc tattttcttt ttaacccaat tgttataatg
    22381 gagagtgtaa aatattttgt gtaaatagaa aaaaggaagt cccttctgta gaatagagtt
    22441 accacaacac attcacagaa gagaggactt ccctatgaac gattttacta cagaaattct
    22501 aaagactcta gcgaacaaag gcgatttgaa tgaattattc cgtgtccatt tggagaaagc
    22561 agtcaatacg cttctcaaaa cggagttaac ggctttccta gattacgaaa agtatgatcg
    22621 cattggtttt aacacgggta attctcgtaa cggctcctat gaccgtacgg tcaagaccga
    22681 gtatggggaa cttcatctcc agattccgcg cgaccgcaat ggtgagttca agcaacagac
    22741 tgttcctgct tatagacgga cgaatgacac gttagaggag accgtcattc acctcttccg
    22801 aaaaggtatt accatgtcgg aaatcgcaga cttgattgag aaaatgtatg ggcattacta
    22861 cacgccccaa accatgtcca atataacaaa atcatttaca gaagaggtaa cggcgtttaa
    22921 tgggcgggag cttcatgacc gttatgctgc tatttatatg gacgcaacgt atattccgtt
    22981 aaagcggaaa accgtcgcca aggaagctat tcatatcgca gttggcattc gcccggacgg
    23041 atcaaaggaa gtattgagct atgcgattgc accgactgaa tccatcacga tttgggagga
    23101 aattttattg gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg
    23161 cttaaagggg atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg
    23221 ttgtgtacac gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt
    23281 ctgtgatgat tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg
    23341 tggtactttt gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc
    23401 gaacgatcac ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc
    23461 tacgaacttg attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca
    23521 gttccaaaat gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca
    23581 aaaattccta ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat
    23641 gctaagccaa ctgattgaga attagaaaca atctgcagta gggaagaatc atttacacaa
    23701 aattattgac gctcccttat aatgagaact tttactaaca taatttatga taaacttact
    23761 atataatcat attaattaaa tagttaggag atctgcttat gtttatatct actcttcaaa
    23821 ttcgaaattt tagaaattat gaatccgaaa tctttcgatt cgataatgag acaaatacta
    23881 ttataggtga gaatgattca gggaaaacca atgctcttac tgcattgaga atcttattag
    23941 atgatagtta ttactatagt agtaaaacac taaaagaatc agattttttt catggaattc
    24001 aaaatgggtg gcaagggcat tggattatta tctctgcgac atttgaagga atcagcgaag
    24061 aagaatttga caatgagatt tgtgcttcca tttctttaaa ttctgaaagt caaacaatct
    24121 tagaagaatt gatttcaaat gcagataaag gtgtaggtag tatttctcta tttattagac
    24181 ctaacaaagc tattagaaag caattatttg atatttccac tgaattagac tctcatcaat
    24241 ttaacgaatt tagaaattct attcgtctat ccgactatga attttactac acatctaaat
    24301 caaatctaaa tttctgtatc aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca
    24361 atatagctag taatcctgaa gaagacgatg aagctctgtt gggctcaaga attgacatgt
    24421 cagatatttt caagcatatt tcggttgttt atgttgacgc tcttcgagat gttcttagag
    24481 aaatgaaaaa caatcgaaac ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat
    24541 catcagataa tgttgagtca ttaaaaacaa ttatacagca attaaatgag actattacca
    24601 gtgttcctga aattaggaaa attggcgaaa atattaatcg gcaactaaat agcataattg
    24661 gttcagtgta ttctcctaat cttcttcttt cctcaactat gtcagatgat atgggttctt
    24721 tagcaaaatt tctttcaatg aagccagaac aaaatatcga cttagactta ctagggttag
    24781 gacatctaaa tatgatttat ttagctttga aaatagtcga atttgaagcg tgtcgttcaa
    24841 gagaactact taatattatg ttgattgaag aaccagaagc tcatattcat catcatattc
    24901 aaaaaactct gttcgaagga ttaaatcttc aaaaaaacta tactcagatt ctcatgacca
    24961 cacattctgt acatttagct gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt
    25021 ttaatggcaa atctatcgtt atgaatccag caaataatct gaactcattt gctaaagaaa
    25081 agttaaaaaa gaacaatcta aatctaataa acgcagtaga gagatattta gatgttaaaa
    25141 gaaatggatt gcttttttct aaaggtgtta tcttagttga aggagatgct gaagaaatac
    25201 ttattcctca aatagtaaaa aaattatttg gtataaactt agacgaactt ggaataggtt
    25261 tgattaatgt tggtagtact tcgtttgaat atatcgctag cttgtttgac gatgatagaa
    25321 tacagagaaa gtgtgctatt gtaactgatt tagatgaaca ggcaatccca tcagaacatc
    25381 gattgtataa aagcactgca caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc
    25441 tttttaatga aaacaattgg gttaattgtt tttatgctaa tacaacattt gaaattgagt
    25501 ttttagatgt gaataaagaa aatattctaa cttatgtcag tccaattata gatgacgcat
    25561 tcaaagataa aaaaacaata actgcttata aagaagaaat acaaagtgaa caatatttag
    25621 aaagaaattt agcgatgtta aaactagcca atcatgttgg taaggggtgg tttgcaattg
    25681 agttaggtga aaaaattgac tttttaatca aaattcctaa gtatattctt aatgctattg
    25741 cttttgcatg tcaggaaata gtagatctct caatttataa aaaaattcta ctccatacaa
    25801 tcgacttgta cgaggaaact acagaattgt cagctttatc agctagttta aaaacaagtg
    25861 atacagctga tttgaatata gcaataattt ctttattaga gtactttggt aaagatgagg
    25921 atgttgtgta tctaatcaat cagatagaaa attacacaaa aatactttat gctgaggttt
    25981 caaaatgaca aatatcttcg aaaaaatcgt aaatgattta aatttagaac aaaaagatgt
    26041 tgtggaaagt gaagataacc tttatgtaaa tgcatgtcct gggagtggga aaacaagggt
    26101 acttacaagg aaaattgctt accaagcaat taaacacgat ggatcactaa aaagaataat
    26161 tgctatcaca tacactaata gagctgctga agaaattaag gaacgcctaa gtttacttag
    26221 tatagatgat gatattaata tttgggtggg gacaatacat cagttttgtc ttgaatttat
    26281 tatttatcct tttaaaatga atctctcaag agtatcaaaa ggatttacaa ttattgatga
    26341 ttatactcaa agagcttacg tcagccaaat taataatatg ctgaatttaa atctcaaaac
    26401 gtatgaactg aacaaaattg atttgtcact ttctcatgat tttaaaatta atgaagttca
    26461 gtatactcaa gtttctgaaa tgtatcacac tatgttggaa aaaaataaag aaattgattt
    26521 tgatatgatt ttactagtag cgtataaaat tttaactgta aacagtaacg ttgcacgtaa
    26581 tattgctagt aatataaggg cgatttatgt ggatgaattt caagatacta gagaattaca
    26641 atacaatgta atagctaaat tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg
    26701 agatattgac caagcaattt atggatcatt agatggtata gctaaatcag ttgaagaact
    26761 agaaatgcta actagtcaca cttttcaatc aaagacgcta catggctgtt acaggtctaa
    26821 tcagagatta gtagacttct actctaattt tcagagttgt ccttacgaga ttgaaagcag
    26881 agggaataat aaaaacgacc gtggttttat aagttatgat tgcaaagtta ctaaggagga
    26941 tttacccaac ataataaaaa atataataaa ggaaaagatt gaaagcggca ttcatgaaaa
    27001 agatatttgt gtacttgctc ctcaacacta tcctctgttt tcactgggtg agtatttaaa
    27061 gaaagaatta ccgtattgca attttgatgc tataaatata tcaccaatta aggtaaataa
    27121 tcatagttta tttttcaagt tagcaatttt gtactttact gaatcaggtg aaaaggttcg
    27181 aagaagaaag ctaattgcaa acgatatttt aat
//
LOCUS       DACDXE010000035        27056 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.035, whole genome shotgun sequence.
ACCESSION   DACDXE010000035 DACDXE010000000
VERSION     DACDXE010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.035"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1602
                     /locus_tag="IWM23_001509"
     CDS             <1..1602
                     /locus_tag="IWM23_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HAP9884158.1"
                     /translation="AEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVTGTVKN
                     VIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAAEPTVDTESAGSSSE
                     GVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKSVEEIPSPVTGTVKN
                     VIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETSGSASVVEAADPNKR
                     VLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPSSAPAKSEAPEAAAP
                     KEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVNSKHTAPHVTLHDEV
                     EVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLNASIDDANQEIVYKH
                     YYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDGKLSAEDMRNGTITI
                     SNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGRVMKLSLSFDHRIVD
                     GATAQQAMNNIKRLLADPELLMMEG"
     gene            1610..3016
                     /gene="lpdA"
                     /locus_tag="IWM23_001510"
     CDS             1610..3016
                     /gene="lpdA"
                     /locus_tag="IWM23_001510"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAP9884159.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            3178..3453
                     /locus_tag="IWM23_001511"
     CDS             3178..3453
                     /locus_tag="IWM23_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAP9884160.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3459..4226
                     /locus_tag="IWM23_001512"
     CDS             3459..4226
                     /locus_tag="IWM23_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAP9884161.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            4394..6223
                     /gene="typA"
                     /locus_tag="IWM23_001513"
     CDS             4394..6223
                     /gene="typA"
                     /locus_tag="IWM23_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAP9884162.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6484..8256
                     /gene="recQ"
                     /locus_tag="IWM23_001514"
     CDS             6484..8256
                     /gene="recQ"
                     /locus_tag="IWM23_001514"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAP9884163.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            8370..8672
                     /locus_tag="IWM23_001515"
     CDS             8370..8672
                     /locus_tag="IWM23_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAP9884164.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8675..9838
                     /locus_tag="IWM23_001516"
     CDS             8675..9838
                     /locus_tag="IWM23_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAP9884165.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9864..13292
                     /locus_tag="IWM23_001517"
     CDS             9864..13292
                     /locus_tag="IWM23_001517"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAP9884166.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            13313..14437
                     /locus_tag="IWM23_001518"
     CDS             13313..14437
                     /locus_tag="IWM23_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884167.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            14427..14864
                     /locus_tag="IWM23_001519"
     CDS             14427..14864
                     /locus_tag="IWM23_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9884168.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14848..15186
                     /locus_tag="IWM23_001520"
     CDS             14848..15186
                     /locus_tag="IWM23_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAP9884169.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            15305..15865
                     /gene="rsmD"
                     /locus_tag="IWM23_001521"
     CDS             15305..15865
                     /gene="rsmD"
                     /locus_tag="IWM23_001521"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAP9884170.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15862..16353
                     /gene="coaD"
                     /locus_tag="IWM23_001522"
     CDS             15862..16353
                     /gene="coaD"
                     /locus_tag="IWM23_001522"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAP9884171.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            16343..17386
                     /locus_tag="IWM23_001523"
     CDS             16343..17386
                     /locus_tag="IWM23_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9884172.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17545..18273
                     /locus_tag="IWM23_001524"
     CDS             17545..18273
                     /locus_tag="IWM23_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAP9884173.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            18389..18634
                     /locus_tag="IWM23_001525"
     CDS             18389..18634
                     /locus_tag="IWM23_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9884174.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18631..18849
                     /locus_tag="IWM23_001526"
     CDS             18631..18849
                     /locus_tag="IWM23_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAP9884175.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18985..19524
                     /locus_tag="IWM23_001527"
     CDS             18985..19524
                     /locus_tag="IWM23_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAP9884176.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19517..21790
                     /locus_tag="IWM23_001528"
     CDS             19517..21790
                     /locus_tag="IWM23_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAP9884177.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21828..22874
                     /gene="holA"
                     /locus_tag="IWM23_001529"
     CDS             21828..22874
                     /gene="holA"
                     /locus_tag="IWM23_001529"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAP9884178.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22963..23217)
                     /locus_tag="IWM23_001530"
     CDS             complement(22963..23217)
                     /locus_tag="IWM23_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAP9884179.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23617..23886)
                     /locus_tag="IWM23_001531"
     CDS             complement(23617..23886)
                     /locus_tag="IWM23_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884180.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(24356..24811)
                     /locus_tag="IWM23_001532"
     CDS             complement(24356..24811)
                     /locus_tag="IWM23_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAP9884181.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24949..26877)
                     /locus_tag="IWM23_001533"
     CDS             complement(24949..26877)
                     /locus_tag="IWM23_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAP9884182.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         9026 a         4690 c         5724 g         7616 t
ORIGIN      
        1 gcagaaggtg aaatcgtcaa atggttcgtc aaaccaggag atacgatcaa tgaagatgat
       61 acattactag aagttcaaaa tgacaaatca gtagaagaaa tcccgtcacc tgttacagga
      121 acggttaaaa atgtgattgt tccagaaggt accgtagcaa atgttgggga tgtattagta
      181 gaaatcgatg caccaggaca cgaagacaat gaaggggaca gtggtgtagc cgcagaatcc
      241 caaactccag ctaaaccggc tgctgaaccc actgttgata cagaatcagc tggatcgtca
      301 agcgaaggag tcttccaatt taagttaccg gatatcggtg aaggaatcgc agaaggtgaa
      361 atcgtcaaat ggttcgtcaa accaggagat acgatcaatg aagatgatac attactagaa
      421 gttcaaaatg acaaatcagt agaagaaatt ccgtcacctg tcacagggac agttaaaaac
      481 gtgattgttc cagaaggcac tgtagcaaat gttggggatg tattagtaga aatcgatgct
      541 cctggacaca atagtgcacc gtcaactagt gcgccatcag ctgaagcacc aaaagaaaaa
      601 gtggaaacat caggttcagc aagtgtcgtt gaagctgccg atccaaataa acgtgtattg
      661 gctatgccat ctgttcgtca atttgcccgt gaaaaagatg tagatatctc acaagtaact
      721 gcaactggaa aaggtggcag agtaacaaaa gaggacatcg aaaacttctt ggcaggtggt
      781 ccaagcagtg caccagcgaa atcagaagcg ccagaagcgg cagcaccgaa agaagcagct
      841 cctgcagcag aaagcaaacc agctgcacca gcaaaaccat tcaagtccaa cttaggagac
      901 ttggaagaac gtgtagcaat gacgccaaca cgtaaagcta tcgctaaagc aatggtcaac
      961 agtaagcata cagctccaca cgtgacattg catgatgaag tagaagtgtc taaactatgg
     1021 gataatcgga aacgatttaa agaagttgct gctgcaaatg gtacgaaact tactttcttg
     1081 ccatatgtcg tgaaagcttt aacagcgact gttaagaaat acccagtatt gaatgcatct
     1141 atcgacgatg caaaccaaga aatcgtttat aaacattact acaatatcgg tattgcaact
     1201 gatacggatc acggactata tgtaccaaat gtgaaagatg cagaccgcaa aggaatgttt
     1261 gctattgcag acgaaatcaa tgagaaagca aaattagctc atgacggcaa actatctgca
     1321 gaagatatgc gcaatggaac aattacgatc agcaatatcg gatctgtagg tggcggttgg
     1381 tttacaccag tcatcaacta tccagaagta gctattttag gtgtaggaac gattgcacaa
     1441 caaccaatcg taaatgctga aggcgaaatc gttgtcggtc gtgtgatgaa actgtcatta
     1501 agtttcgatc accgtatcgt agatggagca actgcgcaac aagcaatgaa taatatcaaa
     1561 cgtcttttag ctgatccaga gctattaatg atggaaggat gataaacaaa tggtagttgg
     1621 agattttgct gtagaattag atacagttgt aattggtgcg ggacctggtg gctatgtagc
     1681 tgccatccgc gctgctgaaa tgggacaaaa agtagcaatc attgaaagag agtatattgg
     1741 cggtgtttgt ttgaatgtcg gctgtattcc ttcaaaagca ctgatagctg caggacacca
     1801 ttatcaagag tcactagatt ctgaaatgtt tggagtaact agtgaaaacg tcaaactaga
     1861 ttttgcaaaa acacaagaat ggaaagaaaa caaagttgtc catacgttga cttctggtgt
     1921 tggtttccta ttaaaaaaac ataaggttga aacaatcgaa ggagaagcgt tcttcgtaga
     1981 tgatcacaca ctacgcgtca ttcatccaga ttcagctcaa acttattcgt tcaataacgc
     2041 aatcatcgca acaggttccc gtccaatcga gattccagga tttaaattcg gaggacgtgt
     2101 gttagattct actggcggat tagcattgaa agaagtacct aagaaatttg tgatcatcgg
     2161 tggcggtgtg attggtgccg aattgggcgg agcgtacgca aacttaggag cagaagtaac
     2221 gattcttgaa ggctcaccac aaattttacc aacttacgaa aaagatctag taaaattagt
     2281 agaagatgat ttcaagaaaa aaggcgtaac agtcgtaacg aatgcaatgg cgaaagaagc
     2341 agtcgataac ggtgacagcg tgacagtgaa atatgcagtg gacgggaaag aagaatccgt
     2401 tactgctgat tatgtgatgg tcactgtagg tcgtcgtccg aatacagatg atatgggctt
     2461 agaacaagcg ggtgtcgaag taggagaacg aggattgatc acggtggata aacaaggacg
     2521 tacgaacgta cctaacatct atgccatcgg tgatatcgtt ccaggggcgg cattagcaca
     2581 caaagccagt tatgaagcaa aaatcgctgc tgaagcaatc tctggcaaaa aagtagctgt
     2641 agattataaa gcaatgccgg cagtagcctt cactgatcct gaattggctt ctgtaggtat
     2701 gacgatcaaa gaagcaaaag atgctggtat cgaagcaaaa gcatataaat tcccattttc
     2761 agggaacggt cgggcattat cgcttggtaa aacagaagga ttcatccgtc tagttacgac
     2821 gatcgaagat aacgtgctta ttggcgcgca aatcggtgga gtcggcgcaa gtgatatggt
     2881 ctctgaatta gctttagcaa tcgaatcagg aatgaatgct gaagatatcg ccttgacgat
     2941 tcatcctcat ccatctcttg gcgaaatcgt catggatgct tcggaattag cattaggttt
     3001 accaatccat atttgatttg aataatacag gcgaaacgca actgaaaaat cagttgcgtt
     3061 ttgtttttta gaattatcaa taatattatt atgtaaactt aaaaatatga caacttcttg
     3121 gtttattacc acttcctatg ctacactgaa gaggaatatg aaataggaga gttgaagatg
     3181 caagaatatc agtatccact tgatttagat tggacaacag acgaaatggt cattgtaatg
     3241 aagatgtggg aagatctgga acgtgcgaat gaaaaaggga taaacaatga agcttttttg
     3301 aagacttatc agcagtttaa aactgttgtc aaatccattg gtgaggaaaa acgattggga
     3361 cgggaatttg aaaaagcttc cggttattct ttatatcgta cagtgcagca agcaaaaaaa
     3421 aatccaaaca aaactttgag aatgaacggg tgagataaat ggaacagatg attgatcata
     3481 tcaagtcctg gcttgaagaa gctggtgaga aaatcaaatc ttctcaacaa acattaacaa
     3541 tctcgcaaaa atcaaatcga acggatctag taacaaatgt cgatcgaaac atccagaatt
     3601 ttctaatcga aaaaatccat tcctttgatc cagaggcgaa aatattggca gaagaagaag
     3661 ggtgtaatca attatcggat ctcgatggaa gagtctttat catcgatcct attgatggca
     3721 ccttgaattt tgtgattgaa ggggaaaatt tttgtattat gctcgctgtt tatgaatccg
     3781 gcgttggaaa gcttggtttc atttatgacg tgatgagaaa tgaacttttt tggggtggaa
     3841 aaggaatagg cgtgtataaa aatgatgagc cattgcctag accagcaaat aaatccttat
     3901 cggaaggctt acttggtgtg aatagttatc ttttcggtca taataaatat ggaatccgag
     3961 aaattgcgga aagcagcatg gggacacgta tgtacggatg cgctggtctt gaactagtgg
     4021 caatcttgaa aggaaaccac attgcttata tttcaaacct tagcccttgg gattatgcag
     4081 cgggaaatgt cttattggaa gagtttggta tggtaacaag cggtcttttt ggtactcctt
     4141 tgacatttga aaaaagggaa tactatttag cagggacttc ttgtgcatat gcagaaatcg
     4201 cagacctttt gaaaaaagaa ggctgaaaaa aaggtagaaa tcattcacaa aaatgataaa
     4261 aacgagcaaa agattaattt tctcgaaaaa atgaaaacat tttactatat ttcatgaaaa
     4321 aagtctgcta taattagcag tcgagtaaaa tttgccctgt cataaagaac caataaaaaa
     4381 ggagttagtt acattgaatt atagaaacga tatccgtaac gtcgcaatta ttgcccacgt
     4441 tgaccacgga aaaacaacat tagttgatga actattaaaa caatcagata cattagacgc
     4501 acatacgcaa ttgcaagaac gtgcgatgga ctcaaatgcg ctagaaaaag aacgtggtat
     4561 cacgatttta gccaaaaaca cagctgtaga ctataaaggt attcgcgtaa acatcatgga
     4621 tactccagga cacgcggact tcggtggcga agtagaacgg atcatgaaaa tggttgacgg
     4681 cgttgtgttg gtcgtagatg cgtatgaagg aacaatgcct caaacacgtt ttgtattgaa
     4741 aaaagcactg gaacaacata tcacaccaat cgttgtcgta aacaagattg acaaaccttc
     4801 tgctcgtcct gagcatgtcg tagatgaagt tcttgaacta ttcatcgaat taggagcaga
     4861 tgatgatcag ttagactttc ctgtgattta tgcatctgca ctaaatggta cgtcaagctt
     4921 gtcagatgat ccagcagatc aagaaccaac catggcacct atttttgata caattattga
     4981 aaaaatccct gctcctgtcg ataacagcga cgaaccatta caattccaag tttcattact
     5041 tgactacaac gattatgtag gtcgtatcgg aatcggacgt gtatttagag gaacaatcaa
     5101 agtaggagac caagtagcat taattaaatt agacggaaca gttaagaaat tccgtgtaac
     5161 gaaattattt ggtttctttg gactaaaacg tctagagatt caagaagcaa aagctggaga
     5221 cttgattgct gtctcaggta tggaagatat ctttgtcggt gaaacagtta ctcctgtgga
     5281 tcatcaagat gcgttaccta tccttcacat tgacgaacca acattgcaaa tgactttctt
     5341 agttaataat tcaccatttg ccggtcgaga aggtaaattc gtaaccgctc gtaagattga
     5401 agaacgtttg atggcagaac ttcaaacaga tgtatcctta cgagttgaac caacaaactc
     5461 tccagatgca tggacagttt ctggtcgtgg agaattgcat ttgtctatct tgattgaaaa
     5521 catgcgccgt gagggatacg aattgcaagt atctcgtcca gaagttattg aaaaagaaat
     5581 cgacggtgta aaatgcgaac cgtttgaacg cgtgcaaatc gatacaccag aagaatatat
     5641 gggtagtgtt atcgagtcct tgagcttacg taaaggtgaa atgcaagata tggtccatac
     5701 aggaaacgga caaatccgtt tgacattctt aacacctgcc cgcggtttga ttggttattc
     5761 aactgaattc ctttcaatga cacgtggtta cggaatcatg aaccatactt ttgaccaata
     5821 tctgccaatg cttcctggtc aaattggcgg acgtcaccaa ggggcgcttg tttcgatcga
     5881 tacaggaaaa gcaacgactt attctatcat gagtatcgaa gaacgcggta cagtcttcgt
     5941 agaaccaggt acagaagtat atgaaggaat gattatcggg gaaaacagcc gtgataatga
     6001 tttgactgtc aacatcacga aagcaaaaca aatgacaaac gtacggtctg ctactaaaga
     6061 ccaaacatct gtcatcaaaa aaccaaaaca attgacgttg gaagaatctt tggaattctt
     6121 gaatgatgat gaatattgcg aagtaacacc agaaagcatt cgcttgagaa aacaaatttt
     6181 agagaaaaat gctcgtgaaa aagcatctaa aaagaaaaaa taatctgtaa aaggatgaag
     6241 agagaaacca gcttgttata aaaaatagct ggtttctttt tttgtccaac tcttttctgt
     6301 ggagaagtca tctttgtcaa tggacataaa ttcagaaaat acgccgaaag acgtttaaaa
     6361 gacttttaaa aaacttattt ctagagagtt tttgtttcat aataagataa tgtttcacga
     6421 aaggagatca acttgtcctt cgaagaaaat cgaagtaaaa tggcaagaga aaggagcggg
     6481 aagatgaacg acttattaaa acaatacttt ggttatgatg agtttagacc tggtcaaaaa
     6541 gaaattatcc agaaagtcat tgatcaggaa aatgtattag gaatcatgcc taccggaagt
     6601 gggaaatcaa tctgttacca gcttccagct ttactattag atgggcttac tgtggttgtg
     6661 tcccctttaa tctctttgat gaaggatcag gtggacgcgg cgaatcaatt aggtataccc
     6721 gccactttta tcaatagctc gttagatggt tacgaaacag ctagacgctt ccaagaaatc
     6781 gatagacagc agtaccgttt actttatatc gctcctgaac gttttatcat gccggatttt
     6841 atccaagcaa tgaagcgatg gaacgtccgt atgatcgcta ttgacgaggc acattgtatc
     6901 tctcagtggg ggcatgattt ccgcccaagt tatttacaga tggcgaacca gctagatcaa
     6961 ctgccaaatc gtcctgtaat tgttgctttg acagctacag cgactgtgca agtagctgct
     7021 gacataaaaa gacttttaaa aattccagaa aacaaccata ttcagactgg ttttgaacga
     7081 gaaaatctac gatttcaagt aattaaagat caaaaaaaag aacaatatct aatcgaatat
     7141 ttaaagatca acaaaaatca atctggtatc atttatgcag caacgagaaa agaagtagac
     7201 cgtctctatc atttgcttaa aaaattcgat ttttccgtgg gtagatatca tggtggtcta
     7261 aatgagaatg agcgaacaga aatgcaagaa gcctttttat acgatcgctt gcagctgatt
     7321 gtagcgacaa atgcatttgg tatgggtata aataaaagca atgtccgttt tgttatccat
     7381 tatcaaattc ctggctctct agaagcatac tatcaggaag ctggtcgggc gggaagagat
     7441 ggtctttcca gtgaagccat cctgcttttc tctcctcaag atatccaagt ccaaaagttt
     7501 tttatccagc agtcccaaag agaagaaggg caaaagcaaa aagaatatga aaaattaaaa
     7561 gcgatgacag aatacgtgta tattgagtcc tgtcttcagc aatacatcct aaactatttt
     7621 ggcgagacaa gcagtccgtg caaccgttgt ggtaactgtt tagatgacag agaaatagta
     7681 gaagtaacga cagaagcaca gatggttctt tcttgcttaa aacgtatggg tgagaactat
     7741 ggtaaacaaa tgctgatgaa agtcttagca ggatcgaaag aacaaaagct gcaagcttta
     7801 ggttttggac atttatctac ctatggactg atgaaaaacc aatcgcaaaa agaaacgatg
     7861 cagttaattg aatatttgat atcgaatggc tatcttctga ccatcaatgg tgagtatcct
     7921 gttttgaaag taacagaaag agggatacaa gtattgaaag gacaagaatc tgtttatcga
     7981 aaggaaccca agaaagtcca gcagctatcg gatgaagaaa ccgatacact atttgaagtg
     8041 ctgcgggaac tgcgaacgga tctagcatca gaagcaggcg ttcccccgta tgtcgtattt
     8101 tcagattcca cattgaaaga aatgagccga atacgtccat caagcagatt ggagatgctt
     8161 cagataaaag gagttggcca aagcaagctt gataaatatg gcgaagcctt tttatctcgg
     8221 ataaaaaatg cggactctaa cgttctggat aagtagaaag aacgaatacg taaaaatgaa
     8281 taaaaacaac tggttaaatc tgtttttttt gctccttatg aaaatcaaat atgttataat
     8341 taaggaatta gaatgggagg taagcacaga tggattcttt gtcgaaaaaa cttgcgatcc
     8401 aagtattaaa tgatgaagca gaacggatca aacgattaat ccgaaatcaa aacaacagtc
     8461 tctgtatctc acaatgcaaa gccttcgaag aagtggtaga tacacagatg tacggctttt
     8521 cccggcaagt cggttttgct gtccgtatag gagtaatcgg aaagaacgag ggccagctga
     8581 tgttaagtga gctagaacga gaattgaatc agttatatag tgaggtttac caagaaattt
     8641 atgataaaaa agaagttggc aaggaggaat agacttgcct aagaaagtga aaaaaaggca
     8701 tctattagac tatagcatct tgattcccta tttaatttta tgtgtattgg gattgatcat
     8761 ggtatatagt tctacgtcgt atctattatt agaaaacgga caaaatccgt ccgcatcagt
     8821 cattaaccag agtatttttt gggtgcttag cttgattgct atcgcactac tttataaaat
     8881 gaaaacagat gttttgaaaa accagcgttt gatcatggca gccatcgcag ttttgacgat
     8941 tttgctgctg attgttgtgt tttttggtaa ggagatcaat ggggcgaaag gttggctgca
     9001 aattgcagga ttctcgatcc aacctgccga atacctaaaa atcatttcta tctggtacct
     9061 gtctctgact ttatctaaaa gacaaaacag tgtacaaaaa gattttcttg ggactgtgaa
     9121 acgtccactt gcaatggtta tcggacttac tgcattagtc gctattcttc ccgattttgg
     9181 taatgcggca gttatctttt tgattatttt ggttctgctt ttagccagcg gagtcaatta
     9241 tgtgtatact ttgattgtag gagtaggagg cttttgtcta agtactttta cgatctggct
     9301 gatcaatatc acaaacggaa agatcttacc cggacgttta cagtatatct ataatcgatt
     9361 tgctatttac caaaatcctt tctcagacga attaaataaa gggcatcaac tagtcaacgg
     9421 ctactatgcc atgttcaacg gaggactatt tggcagggga ttaggtaaca gtatccagaa
     9481 aaaaggattt ttacaagaag cacaaaccga ttttatttat gcaatcgtag tggaagaact
     9541 gggagtgatt atggggattt tgatattagc gttgctattc tttatgattg tgcgcattgt
     9601 ccttgtaggt atccgttcaa aagatccgtt caattcgctt ttgtgcattg ggatcggcgc
     9661 aatgtttttg atccaagtat ttgtcaatct tggaggaatt actggcgtta ttcctttgac
     9721 agggatcacg ttcccatttt taagtcaagg aggctccagt ttgctgatgc tttcgatttg
     9781 tgtcggcttt gtcctcaaca tcagtgcgga tgaaaaacgc aagagcttag gtttataata
     9841 aaaaaagatg taggagtggt tcgatgaaaa aagttttagt tgctaacagg ggtgaaatcg
     9901 ctgtacgtgt atttcgtgct tgtacagaat taggaattaa aactgttgga atctatgcag
     9961 aagaagatga atattccgta caccgtttca aagcggatga agcatattta gtaggacaag
    10021 gaaaaaaacc gattgatgcg tatctagata ttgaagggat catttctatc gcgaaagaat
    10081 gcggagctga cgctattcat ccgggttacg gtcttttgtc ggaaaacttg aattttgcgc
    10141 aacggtgcga agaagaaggc atcatttttg ttggacccaa actgcatcat ttagatattt
    10201 ttggagataa aatcaaagca aaagctgcag ctatcgaagc ggggattgct tcgattccag
    10261 gcacagatgg accaatcgct tctatagacg atgccttgga gtttgcgaaa caatacggtt
    10321 atccgattat gatcaaggca gctctaggcg gcggcggacg cggcatgcgt gtagctcacg
    10381 atgaaaagag tgcaagagag ggctacgaac gtgcgaaaag cgaagcaaaa gcagcctttg
    10441 gtagtgatga agtgtatgtt gaaaaataca tcgctaatcc taagcatatc gaagtacaga
    10501 ttctcgggga tacgcatgga aacgtcatcc atctttttga aagagattgt tctgttcagc
    10561 gccgtcacca aaaagtagta gaagtagcgc cttgtgtctc tatgaatgaa cagcaacgtc
    10621 aaaaaatctg ccaggcagct gtccaattga tgaaatatgt cgggtatgtc aacgcaggta
    10681 cagtagaatt tcttgttgaa ggagatgatt tttactttat cgaagtcaac cctcgcgtcc
    10741 aagtggaaca tacgatcaca gaaatgatta cagatattga tatcgtaaca acgcaactgt
    10801 tgattgctca aggtttggat ctgcataaag aaatcggatt gcctcagcaa gaaggaatta
    10861 aattaaatgg ttcagccatt caatgccgaa tcactacaga agacccgttg aataactttt
    10921 taccagacac agggaaaatc gatacgtatc gttcacctgg tggttttggc gtacgtctgg
    10981 atgtcggaaa tgcttacgcg ggctatgtag tgacaccgta ttttgattca ttgctagtaa
    11041 aagtatgtac gcatggtgct acctttgaaa cagccattca gaaaatggaa cgttgcttga
    11101 aagaatttag aatccgtgga gtgaaaacaa acattccatt tatgctcaat gtcatcacac
    11161 atcctgaatt ccaatcagga aatgcaaaaa caaccttcat tgatagtaca gcaacattat
    11221 ttgaatttcc tcgactacga gaccgcggaa acaaaacgat gaaatatatc ggtgaaatta
    11281 ctgtgaatgg ttttccaggt atcgaaagtg gcgaaaaacc attttatgaa gaaccacgta
    11341 tgcctaaaga tctaatcaca agaactgatt acgtaacggc taagaatgta ttagatgcga
    11401 agggtgccga tgctttggtc gaatggatca aaggacagga aaatttgttg ttgacggata
    11461 cgacatttag agacgcccat caaagtctgc tagcaactcg tgttcggaca aaggatttca
    11521 agcagattgc acgtttgact ggagaaggtc ttcctgaact cttttcaagt gagatgtggg
    11581 ggggagcaac atttgatgtg gcttaccgct ttttgaatga agatccttgg caacggttga
    11641 gaaaaattcg ttctttaatg cctaatacgt tgctgcaaat gcttttcaga ggttcaaatg
    11701 ccgttgggta ttctaattat cctgacaatg tattggtgga atttgtcaaa gaagcagcag
    11761 cgcaaggaat cgatgtattt cgaatctttg atagtttgaa ctggactcca caaatggaaa
    11821 aaagtatcca agctgtacgg gatactggta aaatcgcaga ggcagctatt tgctatactg
    11881 gcgatatcaa cgatccaagt cgggcaaaat acaatgttca gtattataaa gacatggcaa
    11941 aggaactaga acaactgggt gcgcatatca ttgcaatcaa agatatggct gggctgctta
    12001 aaccgcaggc agcttatcgt ttgattagtg aattgaaaga aacgacagat ctgccaatcc
    12061 atctgcatac tcatgatacc agcgggaatg gaatcatcac ttattctgct gcttcaaaag
    12121 caggagtaga catcgttgac gtggcaatga gtgcaatgag tgggaatact agtcagccta
    12181 gcatgagcag cttgtattat gcactggtca atggtccacg gctgccagaa atcacgattg
    12241 aaaatgcaca gaaattaaac cattattggg aagatgtgcg catgtattac aaaccgtttg
    12301 aaaacggctt gaatgctcct gaaacagaag tatatatgca tgaaatgcca ggaggacagt
    12361 attcgaatct acagcaacag gcaaaagctg tcggattagg tcatcgctgg gatgaaatca
    12421 aacaaatgta tcatactgtc aacttgatgt ttggcgatat cgttaaagta actccatcat
    12481 ctaaagtagt tggagatatg gcactattta tggtccaaaa tgatcttaca gaagaagata
    12541 tttatgaaaa aggcgaaaca ctaagtttcc ctgaatctgt tgtgactttc ttccaaggag
    12601 aactagggca acccgttggc ggatttccta aaaaattgca aaagattatt cttaaaggca
    12661 gacctgcatt gaatgaacgt ccaggtcttt tggctgaatc agtcgacttc aacgaagtaa
    12721 aaaaagaatt ggcagaaaaa attggctatg aaccaaaaca agacgaagta ttaagctatt
    12781 tgatgtatcc gcaagtcttt ttggactatc aaaaagcata taatcaattt ggcgatgtta
    12841 cattactgga cacaccaaca tttttccaag gtatacgttt gggtgaaaca atcaatgtac
    12901 aaatcgaacg cggaaaaacg cttatcattc gtttagatga aatcggagaa cccgatatcg
    12961 aagggaatcg cgtactgttc ttcaatttga atggacaaag acgtgaaatc actatcaaag
    13021 ataattcgat catcagtacg gtccaaaccc gattgaaagc tgaaccgaca aaccgagaac
    13081 aaattggagc aacgatgtct ggttctgttt tggatgtatt agtgaaaaaa ggcgataacg
    13141 ttaaaaaagg tgatacgttg atgattactg aagcgatgaa aatggaaacg gccatcgaag
    13201 cacgttttga cggagaagtc gcacatgtat atgtgtcttc aggagacact atttcgtctg
    13261 gtgacctttt gattgaagtg actgagaaat aataagcgaa aggtggggaa acatgaaaag
    13321 actgggtttg ttttggctga ttctgctatt agttattgtg gcattctaca tggaaccagt
    13381 cttgtttccg ccaaaacttg aagtgaagga aaactctgat caacaaatat caaaccaaat
    13441 gacaacgtgg aaataccagg agcttaaagc aactggtttt tctacttata tcggaaaacc
    13501 gatcgatcag ctggaaaagg attttggcca agcctatgat cgattaacta gcggatttgg
    13561 ttttgaaaca agatattatg aaaataaagc tagtcatctc acatttgaag cgaacatcca
    13621 agacagcaga gtcacaactg ttaaagtact gcaaacaggg cctgctgata tcgcgccgtt
    13681 ttatttaggg atgacgatgc aagatcttac gaaaatcact actatctata ctaatttcac
    13741 ttttgagtac catgatacag aagttggaat cgaactaatg gaagaggata tgaattatcg
    13801 tccgctaatt gcttttgata acgacacttt tgctatttta ttttttgatc aaacaacaaa
    13861 cggcctattt tcagttgtgt atttagataa agatagtctt ttgaaactat tgccatatca
    13921 agtgtttggt gaaggccttc cacattatca agcagataaa tcggctgatt gggaatcaat
    13981 caatcgcgtt aaagaacgaa aaagtacgac tttattaaat atgcttagaa gagaagacga
    14041 acttcccata tacaatcaaa cacttgcctt tcaaaaccac agtcagctgc tactacatga
    14101 ttttttgaca aggcctgagg aaatattgtc agaggaacgt atggctgatt ggcaaaaatc
    14161 gctaggcagt gctcaaacga cagtgaaatt ctcactgacg aatgatgaat tggagaagtt
    14221 aatcagtaaa caaaaattag aaaatactag tgggctattt attcatccgg cgattgacgc
    14281 tactttttcg tttttatatt ggtacagcga tccttacaac catgaccgtt tcatggaccc
    14341 gggaacggat tctgtaggca ttgctttttc taaagaaaat atgttagtct tattgcaaga
    14401 agaaaacgga gaaagtagtg atagcagtga tcgttaacga agagctgttt caactggagg
    14461 atcaatgcct ccaagtcgct gaaatgataa aaaagagtaa aacgatggct tcttatcttg
    14521 aacataaaca gtcaatggaa gaatctgcaa aagtttgcca gttgaaagaa gcgttcttgt
    14581 ctaagaaaga agcatttgaa aatgtagcgg catatggtga gtatgcacct gattatcgtg
    14641 aaaaaaaacg agctgttcga aaagcaaaaa gacagcttga tatggatgag catgttgcag
    14701 ctttccgata tagtgaaaca cagctgcaaa gtctgttgga tcaaattgtc caagtacttg
    14761 ccgaagaagt ttctaatgaa atcaaagtag atgcaggtag cccgtttttt gaaacgaaaa
    14821 aacactttgg atgcggaggt aattgtcatg caattagaca atagtgaatt tgaaataaca
    14881 aaacgacgcg ggctgatcgt atgggtctac agcttaaaac aattaaagaa tctgaaaaag
    14941 ttcggcttgt tgcattatgt ctcaagaaga atgaaatatg tcgtgcttta cttaaatgaa
    15001 gagacattgg aagaaacaga agaacgaatc cgtaaattac attttgttcg aaacgtagaa
    15061 cgttcttacc gaccggatat cgaaatgaat tttgccgaaa aaataggaaa caaaacagta
    15121 ttaaaagacg aaggtttcga aattgaagag ctaaatacag aaatccgatt agctgaacat
    15181 ctttaaaaat ctcttatgag cagtttttaa ataaaaaata acttggataa cgatcgattt
    15241 ttgatcattg tccaagtatt tttttggtat actagtacca gtaccatttc taaggagtga
    15301 gtagatgcgg ataatatcag gaaattatgg cgggcgccgt ttaaaaagtt tagcaggggc
    15361 gaacaccaga ccaactacag ataaagtaaa agaatccata ttcaatatga ttggaccata
    15421 ttttgatgga gagacagtat tggatctgtt tgcaggaagc ggtggtttag ctatcgaagc
    15481 agtatctaga ggctgcagtc atgcggtatg cgtagacaaa aattaccaag cgctaaaaat
    15541 catcaaagaa aacatcgaaa ttacgaaaga accagagaaa tttaccacaa taaaacttga
    15601 tgcggacaaa gcaatcgaac ggctagcagc ggagaaacaa caattcgact acttgtttct
    15661 tgatccgcca tatgcaaaac aaaaaataat cgatcaaatc cataaaatgg aagaactggg
    15721 acttttttct aaaaccgcag ttatcgtatg cgaaacggat aaaacagtcg aattgccaga
    15781 aactgtggct ggattccatc aaataagaaa acagatttat ggcataacgg ccgtaactat
    15841 ctatagaaaa gagggaaatc aatgaaacgt gcattattcc caggaagttt tgatcctttt
    15901 acaaaaggtc acttagatac ggtggaacgt gcggctaaat tattcgatga ggtagtaatt
    15961 ggtgttttta tcaatacttc aaagaaaagt ttgtttcccc cagaagaaag aatgacattg
    16021 atcacaaaag ctgtttctca tcttcctaat gtaaaagtca tgcatcaaga aaatcaattg
    16081 acggtagaaa cagcaaaaga gattggtgca gatgcgttga tcagaggcat acgaagtatc
    16141 aaagactttg aatatgaacg agagattgct cagatgaatc atcatctata tgatgaattg
    16201 gaaactgtct tcttattagc aaaaccagaa tatagtcacg tgagttccag tattttaaaa
    16261 gaagtactgc actttggcgg agatgtgagc agctacttac cgccagtgat caacgaagct
    16321 ttagctggaa agcgtgaatc aaatgaaagc taaacaatgg ttgaaaacag tactgattat
    16381 catatttgga atgttgatca ttggatttgt agtagttccc ttgccgtatt atgtagaatc
    16441 accgggagcc acgatcgatc tgaaagaact aattacggtc aatgataagg aagataaagg
    16501 ttctgggtct ttctctctta cctctgttgg tatcaggcaa gctacaggtt tcttagcttt
    16561 aaaagctaaa atgacccctt ttgaagaatt gatcagtgaa gaagaattga ctggtggggc
    16621 gacaaatgaa gaatataatc aaatgcaaca gtattatatg gattcttccc aaaatgcagc
    16681 aatcgaacaa gcgttaaaac ttgcagacaa gccttataaa atggaattca aaggcgtata
    16741 tgtcatgagt gtagagaaaa catcgaattt ctttggcaag ctttcagttg gagatacagt
    16801 aacaaaagta gacgggaaaa gctttcaaag tacagaagca tttatggatt atgtaaaaag
    16861 ccaaaaagtt ggacaaacaa tcgaaatcag ttatttgcat aatggtgaag aaaaaacagc
    16921 ttctggtgat ctgatcgagt taccaacaga taaaaaggca ggaataggta tcacgcttac
    16981 tgaccatacc gagatcgaat cagatacaga tgttcgtatt aattctggct ctatcggtgg
    17041 gccatcagcg ggattgatgt tcactttaga aatctatgaa caagtcactg gtaaaaactt
    17101 gcgacatggt aagcaaattg caggaactgg aacgatcaac agtgaaggag aagttggccg
    17161 aatcggagga atcgataaaa aagtggcaag tgcggataaa gctggcgtag agattttctt
    17221 tgcaccagat gatacgattt cagacgatgt taagaaagaa tatcctgaaa taaaaaccaa
    17281 ctacgaagaa gcaaaagctg ccgctaaaaa attgaattcc tcaatgaaaa tcgtaccagt
    17341 aaaaacagta caagaagcgc ttgactatct agagaaaatg aaatgaaact aaataattaa
    17401 agaggggttg tgacacgagt tctgtcacaa cccctctttt tcgtcaaaaa tcttcacgat
    17461 aaaaataaaa acaaaagaaa aaattgacgt aattagatgt tgaagggacg attttgccag
    17521 agctcactgt ttcttcaatt ttaaatgaaa ggagtaatga atgattttta ctggtcattc
    17581 tttgaaaaaa gaacaatgat agaaaaaatc agcaagaaaa tactagcttc acccaaattg
    17641 atcatttgga tgtctatgat agttcttatc ttttgtatcg caagtggttt tttattatca
    17701 aatttgctat tttatagaga aacagaagag actgctgcaa aagaaatact ttcttcgttt
    17761 agttccagtg gagaagatcc atcagctgaa ggaaacgatg tagaagagga tgctcgtcca
    17821 aaggtcatgt acaccgatat caaaggaagt gtgaaggagc ctggtatcta ttccttttct
    17881 tcagaagaac gggtctatga tgtactaaag cgtgcaggtg gattattaga agaagcggat
    17941 agcgatcgaa tcaacttttc tgcgaagata gaagatcagc aagtcctata tatccctgct
    18001 gtaggtgaag agctgccaga acatcttgat cagttggcat cctcggaagg aaaacagcct
    18061 acagcagatt ccgagcctcc caaaataaat atcaatacag ccactccatc agaacttcag
    18121 cagattcctg ggatcggatc agtcaaagcg caagaaatca ttcgatttag agaagaaaac
    18181 ggatccttcc agaaagtgga agacctgcag gagatttctg gtatcggtga aaagacagtt
    18241 gaaaaactaa aaaacttcgt tacaattaaa tagaatgatt caaaatcgac tggcacgagc
    18301 aataaatgga cgctaatgga tactaagaat aatccaattc taagagcttg gcaggacgag
    18361 gaggtagtat ttttggatag gaaatagctt gattgttcgc aaagaaacat taaaaaaacc
    18421 aatgctgaat gtctatctcc aaaataaaat atctgggatt catattatga atacggctgt
    18481 cagcggcaat aacagtcaag ctctacgaga acgctttgcc aaagatgtac ttagctatac
    18541 agcagataaa gtatttattc tcattgggac gaatgatcta gcagagcata aacaactttc
    18601 taaagaaacg taccaaaaaa tctgcagtgg atgatagttc agctgaaaaa agactatagt
    18661 tctgatcatg ctttctttat cacgccacca gcagtcgatg agacaaaaca aaaatatcgg
    18721 aataacaagt tgatcaaaga atatatctct gttctcacag aagtagcaaa acgccatgat
    18781 tgtgaagtat cgatttttat acggaatttc ttgaacaaat ggattcgaag aatttttata
    18841 atgggctaat ggatgacgga cttcatttcg gcagtgaagg gtaggatctt ttgtcagaca
    18901 ggatgattcg ttatatttaa agaatatttt gtttaagcaa tgagtacttt ttaatcaact
    18961 tgaaaaaacg gaggaatata aaacatgtta gaaaaatctt caaaagcatg cgatacgaat
    19021 aaagaaaaag ttactgataa gtcacataat gaacgcattc cttgggacca gtactttatg
    19081 gctcaggcag tactgttatc tttacgtagt acgtgtacac gtttagaagt aggagcgaca
    19141 ctcgttcgag aaaaaaggat cattgctggc ggctataatg gagcagtcag tggagatgtc
    19201 cactgcatag atgaagggtg ttacgtcgtg gatggacact gcttgcgaac gatccatgca
    19261 gagatgaatg cactcctaca gtgtgcaaaa cttggtatcc cgacggaagg ttcagaaatc
    19321 tatgtcacac attttccatg tttggcctgt acaaaggctt tgttgcaagc tggtgtgaaa
    19381 gggatcaatt atctccatga ctatcgaaat gatccttatg cccaagcgtt gatcgaacaa
    19441 ttaggtgtat ctgtacacca agttgcttta gactcacagc atttttcaaa acttcaaaat
    19501 gaattaacag gaaaacatgt ctagcgtttt agaaaggcat gttcgaaatc ggttgatttt
    19561 tccggcaatt acgtcgatga ttatctgttt gttgtattac actgacaatt ggggttatcg
    19621 tttgcttctt gccggaattt ttttatggtt ttcgctaaaa gaaggacgtt ttatcgcgta
    19681 cttgtgtatt ttgtgtagca taacggtatc catttcttat aggctacaag agtattcatt
    19741 tttagaggaa aaaacaacat ctgcgcaaat tactgtattt acagatacga ttcgtgcaaa
    19801 tggtaatttt ttgacaatgg aaggaaggtt atcagagaat aaacaaaagc tccagctttc
    19861 ttatcaagta aacacagaag cagaattgaa aacatggctt aacttccgag gaaagggaat
    19921 agaattgact gctgaaggcg agttcatctc tccagcaaaa aagcggaatc tttataattt
    19981 tgaccaagaa gcttacttca ggaaccatag aatttctgga cggttccaaa ttcaaaagat
    20041 tgaacagatc accatgcaaa atcactggcg aaactggctt cagagaaaaa gaggatcttt
    20101 tatctccttt gtccaatctg aatttccgca aaaaacaagt gtatatatta attctttatt
    20161 atggggctat aaagatgcag gattcagaga accagaagaa atttttaggg agagtggttt
    20221 gctgcatctg ttcagtctat cgggtctaca catccaattt tatttagggt ggctgtatta
    20281 tttatttcga cgtatcggat ggccactaca cgtcagtatt attccgttga gtctactaac
    20341 tatctgttat gtttctattg caggaagttc tatttctgtt ttgcgtgcta gtttgctgtt
    20401 tttattgcga ttgctagtca aattgctgaa taagcattat tccaccatgg acttatttgc
    20461 agttgtcttt tggcttcttt tactatttga tcctcgattt ctttttcatg cgggtggaca
    20521 gcttagtttt ttcatgagtt ttttgtttat cctgatttct tttgatggag attggttgaa
    20581 aaaaacggcc tctcacgtcc tgtttactat gatcacgatg ccgttgattg tatggcattt
    20641 ttatgagtgg cctattttag gtagtctttt tacattactg attgcaccgg tattcaagtt
    20701 tctttttctt cctggtgtgt tatttctcac actcttcaat cgctggctgc cggaaacttt
    20761 acttttgctg atagaagagg gattaatctt atttgagcga ataatcgatt tttcttcagg
    20821 gtcgtctctc attataggac gtttgcccct tgtgctatct gggatgtata ttttcggatt
    20881 actgatttgt atggataggt tgaaagaaca acctgtgaaa cacttgtttt atgttggtct
    20941 tttttctttt atttttttga ctttgccatt tttacgcttt tcttcaacga tcgcttttat
    21001 cgatgtcggt caaggagaca gtatcttttt gcaaagtcct tttcgaaaag aaacgatttt
    21061 gatcgatact ggaggtcagt tacactttga aagagaaaag tgggcgcaag gtatgatacg
    21121 tcctcctgct gaaagaaata tcgttccata tctaaaaggt caggggataa gtgtaatcga
    21181 taaactcatc ttgacacacg acgatacgga tcatgtggga gaattaccca cgttatctca
    21241 gcattttact atcaaaaaaa tttatatcgg ttggggcgct ggacgaaatg agccattaca
    21301 aaggcattta tctgaagctc aaaaaaatgg tactgagatc attgaaatca aacatgggga
    21361 caggatcagc ggttattatg atctttatgt attgactcct tttgaaaagg gagagggaag
    21421 aaatgaagat tcgattggtc tatggatgga atataacaat aggcgctttt tatttttagg
    21481 agatttgaat caagcaatgg aaaaacaatt gcttttgatt tatcctaatc tgaaagccga
    21541 tgtagtcaaa ttaggtcatc acggtagccg tacgtcaagt aacacagatt ttctttccca
    21601 tattgaagca aaacatggga ttatatcctg cggcgtaaat aatcgttatg gtcacccgca
    21661 cagcgaagta ttggagacac tagaagaaaa tcagataaaa acatggcgaa cggatcaaca
    21721 aggaatgatc acctatcgct ggcatccagt ttttcatcct catgggctag tggaaacaat
    21781 gatagactag taattctcat ctgctcatgg taggataatt tttgaaagtg gtgagtaatt
    21841 tgagtataca agcagagtta caaaaaatac gtaaagaaaa acttgcatct tgttacttga
    21901 ttttaggaac agaaaagttt ctccaagatc aagttcggac agaaattgtc aaaaaaatcc
    21961 aattagaggg cgaagatgac ttaaattttt taagttttga catggaaaat gcaagtttag
    22021 atgaggtagt agctgaagca gagacgttgc cgtttttcgg agatcaacgg ttagtattcg
    22081 tagagaatcc ttatttttta acaggtgaaa aagtaaacaa tggaatcgaa caaaatactg
    22141 atttattgac ggcttatttg aaagaaccat tagaatcgac tgtattagta ttctttgcgc
    22201 catatgaaaa gttggatgaa cggaaaaaag taacgaaaca attgaaaaaa acagcaatta
    22261 tcatcaatgt ccaacagttg aatgaaaaag aagtacgtca atatctgatg aataccttag
    22321 agaattcctc aatcaaaatg gaacgaagtg caattgattt attcttgagg ttgaccgact
    22381 tagatttatc caaaatgatg agagagttac agaaattggt cctttatgga caaaatcaac
    22441 agcaaatcac ggtaagagaa gtagaagaat tagttcctaa aacgttagaa cataatattt
    22501 ttgacatgac ccaatatatt ttaaaaggga agacggaaca agctttgcgt ttatacgaag
    22561 atttagttat gcaaggggaa gaaacaatca aaattaatgc gattttgctt tctcaattac
    22621 gtttattttt acagacaaaa tttcttgtaa agattggtta tcagcaggca aatattgctg
    22681 aaacattgaa aatccatcca tatcgtgtaa aacttgccat gcaggaagta aaaaaattcg
    22741 atgaacattt gctggtacag ttgtttgatc aattagttga gatggattat caaatcaaaa
    22801 cgggacaaat cgaaaaagaa ttaagttttc aactattcgt tttgcgcaca gggcagttgt
    22861 taagaaatcg ataagaagaa agacgagggc aaagcatgcg ggaaggtcgt ttgtttttgt
    22921 cctttttttg acaaataaaa aaatcggccg aagccgattc ttttatttag cgattttttt
    22981 gcttaaacga gatttgtcgc ggttagcttt gtttttgtgg attagacctt ttgattcagc
    23041 catatcaata gctttaacag cttctttata taacgcatct acgttgtcag cacctgaagc
    23101 tacagcatct tcaaattttt tgattgcagt acgcatagcg cttgtttgag atgaattttt
    23161 cacattagcg ttttcgcttg tacgaacacg tttgattgca gattcaatgt ttggcattag
    23221 tttcacctcc gataacttaa gttcttacgc gtgttaccgc ataattcaac attcattatt
    23281 atacctaaag gcagcgtgct ttgcaatatg aacatgacaa gtttttttcc ttaacagtct
    23341 tttattagga ttttttttca gtaaaaacgg atagtacaag ctgaaaaaaa tcaaaaagac
    23401 gctattgtgg gaggcaatag cgtcaaacca aactagattt ttatttatct atttaaacaa
    23461 agtttgaatg ggaatgtttg gtactaagtt attatataac aaagagaacc gtttgcaaag
    23521 ttttttttga aaagggatac gattccctgt tgttctttgt ttttctatta agaaactgca
    23581 gttattttat aaccagtatc ctttgatcag taaaggctat tctggtgtac cgattatttt
    23641 aatgtattca tcgatatttt ttatcaacat tttttttatg accaactggt tccgacggtt
    23701 ccatttgcca cttacttcca gcacatattt atttaggggc aaacgtaaga tatattttgc
    23761 caaatagcga tctttaagca ggccattgat cacacggtcc tcaagctgca agttgatccc
    23821 agtcagcatc attggctgtt cttgtaaaac gtacgtatca attaaaagac cttcaaaagt
    23881 ttccacaagc gtcttccttt cttttcaaag taacctgaaa acaggcacta attcttccat
    23941 tttgatattc gttgaagtgg atgcgatcat tctttctttt gtccactgta atctagtata
    24001 acaaaaaaga gcagaagagc caaaaagaaa aaagagcatc gaattctata ggaagtctca
    24061 ttggcacggc ctaaattatt acaaaacatt gattaaaaaa agcccctgca tcgcaggagc
    24121 atcccctata tcgttttaat aggtcaatca aaaagcagta tcaaacacat tttgtatcaa
    24181 atcaggaaat aatagataag ctaagcccag aatcaaaagt accgctagaa gaaaccagcc
    24241 gattgttcct atgaaatact ttaatttttc gaacattgta tcacctcgtt gtcagtatat
    24301 cacggatcag taaattgggg gtgttaagaa tctcctaaaa tagacaaaat ttgttctatt
    24361 ttcctaaagc aaatacttgt aagatacagt aagtgatacc agaagcaatg accggtccgg
    24421 cggcgattcc tcttaagaag acaacaccta aaatcgtgcc gaaaacaagt gcaacagtga
    24481 tttgtggatc agaagcaatc aatcctacac ccttggaaga taagacagcc actagcccac
    24541 cgcaaaaaac agctacccag ccaatagggg atttaaaggc ttctaataga tcacgtagac
    24601 cgatttctcc tgttgcgata gggattagaa tagccgctgt gatgatcgtc accccaatat
    24661 tgattccttt gccttgaagc caagggaaaa aggaacggga ggctggtatg agcttcataa
    24721 tcaaaacaaa agctgccgct atcatcagac tttgattttt agctaaaatt gctacaagta
    24781 aaataattcc taaaaaaagc caactttcca tgaaatatat tcctcacttt cttcgaaaca
    24841 atcataacat gtttttagaa agttacaaaa gtaggaattc atccttcaag acctattcat
    24901 aagctgtttt tttcataaaa taaacaaaat cataaaaaaa cactttgctt atttgacaga
    24961 agacagccgc gctttgtata gtggacaaga cttacatttt tttgaagaat cagaaagatc
    25021 aggtaaaggt cttagtaaac gcaagccatt cagaataacc aatattgtgc ttccttcgtg
    25081 tcctacaact ccaagaggta gatttaaaaa ttgcaggaag ttagaagcaa tcaacaagag
    25141 aataacaaga atcgaaaaaa taatattttg tttgatgatc cgccgcattt ttaaagatag
    25201 ccgatgactg tagacaaatt tatttaagtc gcttttcatt aagacgatgt ctgcgatatc
    25261 catagcggca tctgtccctt gccccatagc gacacctaaa gaagcggctg ctaaagcagg
    25321 cgcatcattg actccatcac caaccatcgc atttacttgg taaagttcat tctctttttt
    25381 gactagttgt gttttttctt ccggagtaca gcttgcatag taatgttcaa tccctacttg
    25441 ttcagcaatg gcctttgctg tgccagaatg gtcgccggtt atcatacttg tatggatgtt
    25501 ttgggattta aagtaggaaa ttacttgttg ggtatttgct tttggaatat ctagcagacc
    25561 taaaacagca attaactgct gatctttaga aagataaatc acggttttgc cttgattttc
    25621 caaacgttcg atcatttccg caatctcttt gctgattatc atcgtctcag gatcatatgc
    25681 gtgcttgcca actttccatt gcgcacctaa ataggtagtt tgtaatccaa aaccagtaat
    25741 ttcttctatt ggtaagtatt ggatttcttc aggtattgtg caatcgaaat attgcaaaat
    25801 cgcatgagca agtggatgcg tcgttttttg ttccatagca acaagaagtt gttgtgccaa
    25861 atccttttca tcaaagaaaa agctgtcagt tacaactggt tttcctttag tgagcgttcc
    25921 tgttttatca aaggcaatcg cttttaattc agaaagcttt tctaaatgga taccgccttt
    25981 gattaaaatc ccattttttg cagaatttga aagagcagct agagaagctg gtgtagcaga
    26041 tgcgacaagc gcacaaggag aagccacaac taataatacc attcctcgat aaaagctttc
    26101 atcccaactc caacctagga aaaagtgggg gagaaggatc attagaggaa cagcaagcaa
    26161 aatcaatttt acataattat tttcgattcg ttcgataaat gaagctgttt gagttggcgt
    26221 attctgcgct tcttctacta attggataat cttagcaaag acagtttcgg aactcgtttt
    26281 tgttactcga atcgtgatgg gatttccttg attcagcgtt cctgcataca tctcatcgcc
    26341 taaatatttt tctacaggca cagattcacc gcttaatgca gactcatcga tcgttgcctg
    26401 tgtgctttct aaaaagccat ctatcggaac agctgctcct ttagcgacaa ataccaagtt
    26461 atccaccttt agttgagaaa catctacttc ttgcatttgt ccattttctt ttaataattg
    26521 ggctttttgt ggttgaagat tcatcaaagc agtaatttct ttttgacttt tattagttgt
    26581 gtattcctct aatgcaccgc ttaaacaaaa gatgaatgtc aacattgctc cttcaaagta
    26641 attaccgatc aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt
    26701 ccgatcattc cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat
    26761 ggcactgata taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa
    26821 gatcataaat aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt
    26881 tattagacct ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac
    26941 agctaaatga gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta
    27001 tttataatga ttattattaa agaaaataaa aaaccagaac atttctcaaa taagag
//
LOCUS       DACDXE010000036        27018 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.036, whole genome shotgun sequence.
ACCESSION   DACDXE010000036 DACDXE010000000
VERSION     DACDXE010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.036"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..74
                     /locus_tag="IWM23_001534"
     tRNA            2..74
                     /locus_tag="IWM23_001534"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IWM23_001535"
     tRNA            78..150
                     /locus_tag="IWM23_001535"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IWM23_001536"
     tRNA            155..225
                     /locus_tag="IWM23_001536"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IWM23_001537"
     tRNA            238..311
                     /locus_tag="IWM23_001537"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IWM23_001538"
     tRNA            323..394
                     /locus_tag="IWM23_001538"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IWM23_001539"
     CDS             660..1853
                     /locus_tag="IWM23_001539"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAP9884183.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IWM23_001540"
     CDS             1983..3455
                     /locus_tag="IWM23_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9884184.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IWM23_001541"
     CDS             3538..4326
                     /locus_tag="IWM23_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAP9884185.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IWM23_001542"
     CDS             complement(4393..5061)
                     /locus_tag="IWM23_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9884186.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IWM23_001543"
     CDS             5176..6888
                     /locus_tag="IWM23_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884187.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="IWM23_001544"
     CDS             6891..8660
                     /locus_tag="IWM23_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884188.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="IWM23_001545"
     CDS             complement(8865..9431)
                     /locus_tag="IWM23_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9884189.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="IWM23_001546"
     CDS             complement(9431..10387)
                     /locus_tag="IWM23_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAP9884190.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="IWM23_001547"
     CDS             complement(10387..11043)
                     /locus_tag="IWM23_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAP9884191.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="IWM23_001548"
     CDS             11413..13431
                     /locus_tag="IWM23_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9884192.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            13858..16272
                     /locus_tag="IWM23_001549"
     CDS             13858..16272
                     /locus_tag="IWM23_001549"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAP9884193.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="IWM23_001550"
     CDS             16620..18173
                     /locus_tag="IWM23_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAP9884194.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="IWM23_001551"
     CDS             complement(18554..20338)
                     /locus_tag="IWM23_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884195.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="IWM23_001552"
     CDS             complement(20353..22074)
                     /locus_tag="IWM23_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884196.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="IWM23_001553"
     CDS             22174..22935
                     /locus_tag="IWM23_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9884197.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            23902..24170
                     /locus_tag="IWM23_001554"
                     /pseudo
     CDS             23902..24170
                     /locus_tag="IWM23_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            24842..>27018
                     /locus_tag="IWM23_001555"
     CDS             24842..>27018
                     /locus_tag="IWM23_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884198.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVL"
BASE COUNT         8628 a         4755 c         5640 g         7995 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttattgtta tttttatata ttctttttaa
    23521 gaatgattgt ttttttatag aaattttaaa aaataaaaca cgttattgat ggatagttag
    23581 tttgttattt ggtaatatta tagaagttga ctggtagtga tttatttttc gtcgttttat
    23641 aaaagtgtta ttttttggaa aaattcacaa atatgtaacg aatgtttttc tggaatttgc
    23701 tgtgttaaaa agcagtatga ctaatataaa tactattgtc atttatgcaa ttttttatgg
    23761 atagtaattt tagaggggaa tcaatgaagg aagaatagaa gggaaaaata gttgcaaata
    23821 cgccagttag caaaaatagc aatacatctt caagctttaa aattcgagag aatcatcttg
    23881 gtagtggaat caactacaac aaaaggatta aaccaattaa tagatagaat taccaatgga
    23941 aaaatagaaa aggtagttat tctatacaaa agttgtttaa ttcgcttcgg ttatgaactt
    24001 agtgaaaccc tttgcaatat atggtacaga atatagaaat tattgacatt acagaaaatg
    24061 atgaagattt aatacagatt gtcactattt gtagatacct tttacaagga aaccgagcca
    24121 ataaagctaa gaagatgatt aaggagttga tggaagacga tacaagttaa gaaagtgaaa
    24181 ctacgcccaa ataaagagca aaaagagtaa ttggacgtgg aaaatgcaag aaatgaacca
    24241 tcgattcggt ggacagttta ttagtaatga cggtttgaga acatccatga cgagaatgaa
    24301 ggaacatcct aaatgcgttt agttgaacca agtgccaaat aatatggcca aataagcgat
    24361 cagttatcga tgaatcccaa gtattctaaa ctatatattc attttgatgc taagcattga
    24421 tatatttttt tgggattgat gaaggttaac caatacaggg attcactgat gaaattctag
    24481 agtctgacct tggtatcaac gatttagtga ttgtttctaa tggaaaagta taaaagtata
    24541 taagtacatt aacaaaataa ctattagggt tacgctccag cttgtgcaag gcactggcag
    24601 gaggaatcgt gaaatcataa ttatggttaa gccacacgga caactaaaag tcatacaaag
    24661 ctatcgaatc aatcaacaga agacaatcgg caatcattag ctttgtttac agcttacatt
    24721 gcaacgcttg agttgaaccg aaaatacgag ggaggtaacg cagccatttt gagttgggga
    24781 atctttttag aaaattatcg ggtaccttgt tgtttagtca aaattataag ggggagtaat
    24841 agtgaggaac aaatttcata aactgttaag tatgtttcta gttatgctta ttgtgatcaa
    24901 tcaatttggg aacataacgg ttctagctga gtctttagca gaacagccag agcgttcgct
    24961 gacgatcgaa gttgatcaag aggaggctgc tttgggtgag cagttccagt tgcgagtcag
    25021 aggaacacca gacctgctaa atgcattggt cattgataag agtgaggatt tgattgaagg
    25081 ggaagtgaag caactcaatg attacgaacg tctgattact tattcttccc agcattcagg
    25141 taaatacgaa ttgaccgcaa gaagcggtga tattcaatca aatacggtct caatagaaat
    25201 cagtgtacca gaagaaagca caagtgagtc ttttaatgta ccagaagaaa gcacaagtga
    25261 atcttttaat ataccagaag aaagcttaag tgaatctttt aatgaagcca gatcatctga
    25321 gccatctata acgcaggaat ctcagattga aagacaagag gagctcttga attatgacat
    25381 cggcatctct gctgagcgcc ccactttgcc gagcaggcat acacaaacgt taaagggata
    25441 cattcaagga ggcgctggcg atattcttga agaatatggg gagatggtcg tacgagttcc
    25501 tagagcatcg attggcttaa ttgagttcat tagagtggag gtcgctgatg attcaccttt
    25561 aaagccactg gcattttacc aagaaggcac agacgttgtt tttccattta cctttgatta
    25621 tgaaggatta aatgaagccg ctcagtcgta tacattcagt atcacgtttg aagttgcttt
    25681 ctttcaatca ggcggtactg agccaaataa tgactttcaa gtgagtctta cttatgctga
    25741 gcgagcagca aacacggaat tttcaattac gcctattcct tcatcaagac ccattttttc
    25801 gaaattatac tatggctcta gagaaatcga cagccaagga aatcttggga atctgctgat
    25861 tgacgggcaa gacgtgacca acagtaataa atttattttg caggtaaatt atgaaggatc
    25921 acgaaaagac ggtgtagtgg ttaccgatgt actaccgcaa ggaacaactt tggcaccgaa
    25981 tccgactaca tggagttcca ctggaaatag agaacctatt tcaggtgggg ttcggatcgc
    26041 agaagtaacg aatccaggtg aagcaaacgt tagaactcat tatgtttccg aggaatttgt
    26101 ggacaaagta tcttttaata aggaaacgaa tacacttgtt gtggactttg gtaatctagc
    26161 agagggtcaa tcgtatttta ttgaatatgc tcttgaagtg accgatgtcg acttgttcaa
    26221 tgaaggaaga caagaggatg ggttttctcg gaatcgagcg agtatgacct acaataatca
    26281 ggtaatgagc cgagactacc gcttgagaat ccctgaaagt aatcttccgg caatctcttt
    26341 aagaaaaagt gtggacaaaa atgttatcaa tctcaatgaa aatgaactaa cgtatactct
    26401 gcatgtctcg atgttttctg gaacaagaaa tacgcttgtt attgaagatg ttttggaaga
    26461 aggcatggaa tttgtgtcat ttctatcaga tattccctct ttcattgatg ccgaagtcaa
    26521 taatggaata gttcgtttca gtgcgaataa tacgattggt ggtccgacaa gttttaccct
    26581 ctcttttaag gtcaatgtag aggcgtatgc ggtcggcgat caaattttaa actttgcaac
    26641 gcttgtgaat agtgacaatc gattgaatac gagtactgta agaacaagga aaatcgatgg
    26701 gcgtattaaa gtaattaaaa ctgatgagaa tggccaaagg ctggcaggag caaaatttga
    26761 aattttatca agcgattcac aagtgataca agaaggtata acggcagaaa acggagaatt
    26821 tttaagtgaa ccgcttcctg taggtagcta tcaagtccga gaaacagaag caccggaagg
    26881 gtatctttta gatgcgacgg tacatgatgt ttttataaat gaatactcca tagaagaagt
    26941 gacgttaacc attgaaaaca tccttgatac gggtggtgtt gaactaatta aacatgctga
    27001 aggcagtggt gaagtcct
//
LOCUS       DACDXE010000037        25787 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.037, whole genome shotgun sequence.
ACCESSION   DACDXE010000037 DACDXE010000000
VERSION     DACDXE010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.037"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            438..992
                     /locus_tag="IWM23_001556"
     CDS             438..992
                     /locus_tag="IWM23_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9884199.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(1165..1638)
                     /locus_tag="IWM23_001557"
     CDS             complement(1165..1638)
                     /locus_tag="IWM23_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9884200.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1650..2186
                     /locus_tag="IWM23_001558"
     CDS             1650..2186
                     /locus_tag="IWM23_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9884201.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            2226..2597
                     /locus_tag="IWM23_001559"
     CDS             2226..2597
                     /locus_tag="IWM23_001559"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9884202.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2881..4152
                     /locus_tag="IWM23_001560"
     CDS             2881..4152
                     /locus_tag="IWM23_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAP9884203.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            4397..5422
                     /locus_tag="IWM23_001561"
     CDS             4397..5422
                     /locus_tag="IWM23_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884204.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5716..6825
                     /locus_tag="IWM23_001562"
     CDS             5716..6825
                     /locus_tag="IWM23_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAP9884205.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6908..7804
                     /locus_tag="IWM23_001563"
                     /pseudo
     CDS             6908..7804
                     /locus_tag="IWM23_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7820..8644
                     /locus_tag="IWM23_001564"
     CDS             7820..8644
                     /locus_tag="IWM23_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884206.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8676..9986
                     /locus_tag="IWM23_001565"
     CDS             8676..9986
                     /locus_tag="IWM23_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAP9884207.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            10134..11393
                     /locus_tag="IWM23_001566"
     CDS             10134..11393
                     /locus_tag="IWM23_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9884208.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            11403..12275
                     /locus_tag="IWM23_001567"
     CDS             11403..12275
                     /locus_tag="IWM23_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884209.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            12287..13117
                     /locus_tag="IWM23_001568"
     CDS             12287..13117
                     /locus_tag="IWM23_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884210.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            13157..15340
                     /locus_tag="IWM23_001569"
     CDS             13157..15340
                     /locus_tag="IWM23_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAP9884211.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            15401..16621
                     /locus_tag="IWM23_001570"
     CDS             15401..16621
                     /locus_tag="IWM23_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAP9884212.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16618..18078
                     /gene="gtfA"
                     /locus_tag="IWM23_001571"
     CDS             16618..18078
                     /gene="gtfA"
                     /locus_tag="IWM23_001571"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAP9884213.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            18155..19261
                     /locus_tag="IWM23_001572"
                     /pseudo
     CDS             18155..19261
                     /locus_tag="IWM23_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19583..19792
                     /locus_tag="IWM23_001573"
     CDS             19583..19792
                     /locus_tag="IWM23_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9884214.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19785..19934
                     /locus_tag="IWM23_001574"
     CDS             19785..19934
                     /locus_tag="IWM23_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAP9884215.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19969..20753
                     /locus_tag="IWM23_001575"
                     /pseudo
     CDS             19969..20753
                     /locus_tag="IWM23_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWM23_001576"
     CDS             complement(21037..22122)
                     /gene="xerS"
                     /locus_tag="IWM23_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAP9884216.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22456..22689
                     /locus_tag="IWM23_001577"
     CDS             22456..22689
                     /locus_tag="IWM23_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAP9884217.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22686..23093
                     /locus_tag="IWM23_001578"
     CDS             22686..23093
                     /locus_tag="IWM23_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAP9884218.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(24230..24748)
                     /locus_tag="IWM23_001579"
     CDS             complement(24230..24748)
                     /locus_tag="IWM23_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HAP9884219.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         8786 a         3930 c         4795 g         8276 t
ORIGIN      
        1 aattattgac gctcccgaca aaattatgat cttgcgtaaa gttataaaat attaataaaa
       61 atattttatt aatgaaaagc ttggtagcac ggttattcat atgctaattt atttcacttt
      121 atagtaaggg gagttcttag tatattgttc aaacttaaat ttcatataat ttaattatgt
      181 aaattagcta ttctaagaac ttctacggat aactgcccgt ctaaaattta aataagagct
      241 tagaatcttc atataagtaa ggttaatttt tttgtacaaa aaagtcggaa tattctgagt
      301 tactgaaatg atataaaatc ataaactaaa tcccttgatt ttattgactt tcaaaagttt
      361 atatatataa gttaatgatt atggtaaaat atatacataa tcattaactc agaatatttt
      421 taagaaggga aacaatagtg agctataatg tacaaccgtt aaggacaaat gaagagatac
      481 aagattttca gttttggcta agaagaacaa gtaatccgga aagagatagt tttttatttt
      541 tgtttggaat caataatggg ttacgtatgt cagatattat aggattaaaa gttggagata
      601 tacgaggcaa aagtaagcct attattgttg aacataaaac aggtaagcga aaaccgattt
      661 ttatagataa tttgagagaa gaaattttat tatatactga aggtaaagaa gaaaatgatt
      721 ggttatttcc gagtcggcaa caaggtcgac atataacaag ggatcgtgtc tatcagatat
      781 atgcagatat agcagaaaaa ttaggacgag atgatatagg aacacataca ttaagaaaaa
      841 cttttgggta tcactactac aagaaaacta gagacattgc taccttaatg tttattttta
      901 atcatagctc gcaagcaatt acaaaaaggt acattggtat aactgaagat gaaataggag
      961 catcacttcg tggatttaaa ctaggtgtat aatttaaaaa tttttataaa cagtaaagac
     1021 aaagattaaa agctattttt atctagaacg attttatttc ataaagttcg cggaataaaa
     1081 gctggggtta ctggtacttg gtataggttc gtgttatata aactaagtaa ttaaataaca
     1141 tttttaggtt ggaaaaggat gaaattaaaa aaagagaatc aatacggtct caaaaacgca
     1201 aacgctgcgg aataagaaaa atactgataa ctcaacgttt agcagactat ctaataggga
     1261 tagtctgctt ttttcgtttt tgaccatact tttgaccttc tgcaaacttc cttgcttttc
     1321 ctctgtagaa ctcatgaaat ctgcaaaacg aattgccgtt tcctctgtct gctttttcgc
     1381 gatatgggtg tatatatcat ccatagtggt tttaatatct gtatgtccaa gtcgaacata
     1441 cacttctttg acagacgcac cagattcaaa gagcagacta caatgggtat gacgaaaacc
     1501 atgaagtgtg atatgtttta agttattctc tttaatgatg attttcaaat tgtgatttac
     1561 atagtctaaa taaaggggct tattttcttc tgatgaaaac aaaagttgtt caccatcttt
     1621 tataggtttg attctcattt ttagatatta tgcccgcacg tattcatcac atttctattg
     1681 tgaatcgctt tattcgaccg acatttgact tttatcataa tatactcggt ttaaaattac
     1741 taatgaaaac aattaatcaa gacgatcata ccatgtatca cctgtttttt tcagataatc
     1801 accaccgtgt gggaactgaa ctgacttttt ttgaagtaca agaagggaac aaccagtttt
     1861 ttggaaccaa tacgattgaa cgaacgatac taaaagttcc ttccgaagca tcattgcatt
     1921 tttgggaaat atattttgaa acacaaggcg tttgccatta tggaatcgaa tcattttctg
     1981 gacgacctat cttacgtttt gaagggccag atgcaacacg attagcttta gttcccttac
     2041 gcgaatttga agatattgat gattatttcc cgtatgtcac agaccagatc cctacagagc
     2101 atgccatttt aggaattgat gccattcaac tacgcgtaca atattccgat gcgactgaac
     2161 gagagttatt gtcggtaggc tggtagcaca aagaatacgt cagtttcttt tcgggtcatc
     2221 aacgagtgac aatcttagaa aatcaagaca ctcagttcta tcaagaagtt cagattattc
     2281 aagatcgtga gaatccagtt gccattgaag ggattggtag cgtccatcat gttgcgtttg
     2341 gcgtagaaaa taaatctgat ttacaacgca tcgacaaaca attacaagag cgcaatttta
     2401 taaattcagg aataaaagat cgtgaatttt ttgtatcact ttattatcgt gacccaaatc
     2461 aattattgat tgaaattgct acaggggaag gaaacctaga tgcaaaagcg tatgaaaatc
     2521 aaagtccacg ctttgaagaa attcctttat ttttaccgca gtacttaaat ttcattcgtg
     2581 aacaagtgga gcaataatta gctaatcaaa tccatgacga agagtaagag gttgactcat
     2641 aagtcaaaaa ataaagaaaa acgcatcaaa tccattctaa agaatgttga tttgatgcgg
     2701 ttcctatttt tataaactat cattttcgac taataaaact acttatgagt cagcctctct
     2761 ttttttcttg atttttatac ctctttctag gtaaactaaa catctgccaa aaataccgtg
     2821 tctcattgaa acattctcaa ctatttcaaa ataaagggga gtaccaaatg aacgagtata
     2881 ttgaaattat ccacgcaaga gaaacaactt aaaaatgtca gtctcaaaat tcccaaaaat
     2941 aaaattagta tttttaccag cgtatctggt tcaggaaaat catcgattgt ttttgatact
     3001 attacccaag aagctggtcg tcaactgaat gacacctatg atagttttac ccgtttattt
     3061 ttacctaaat acagtcgtcc agaggttgat gagattgata atctttctac ggcgattgtg
     3121 gtcaatcaaa agaagattgg tggcagtgcg cgctcaacgt tagggacgat tactaacatc
     3181 aatgcgttat ttcgtgtgtt gttttcatgt tttgcaacac ctagtttagg atatgcaaat
     3241 gcttattcat ttaatgatcc tagtggaatg tgttctgtct gtcagggaat cggtaagacg
     3301 attgcatttt taataggatt gctttatttt tcggctattg ataatgctga ttttgtattt
     3361 tctgatccat ttaccgcaga agttttgtct ggaattgagg aacaggcaaa gaatcatggt
     3421 tattttgtat tggtgcataa tgttaattca gcagaagatg ttaaaactat tcagagaaat
     3481 tggcgttttg aggggttcat tgttgttggt gtacgtgtaa aagaattttt ggaattaaat
     3541 aacgtttttg atggtcctgt tgtttatatt gatacgcatc tttcaaaaga gacattaaga
     3601 gaacttgaac aacaaatgaa tcgtttattt gtaaatacaa atgattacca agctagtaca
     3661 ttggcagtgg aacatttgat tcattatggt catgagaaaa tagcgtttct ttcttttgac
     3721 tacattgtag gtgaaccaag tgttattcaa gaacgacatc aagcttatat cgatacatta
     3781 aagaaatatc atttaacctt taaatcgcat tggatatata acaactctga atttgagcga
     3841 atttatcaag aattagctga atttactgca attgttgtta ctggagatta tttagcggct
     3901 aaattagtaa aatttttaaa aaataagaat aaacgagtga tggaacaatt atcgattatt
     3961 ggatttgatg atattacttt tgcagaatta atggatcctg cgttgacaac aatacgacta
     4021 aatccaggta acaagggggc gattgctttt caccaactga ttggactggc caaacaagaa
     4081 gaaataaaaa atcaagtact actattagag ggagaattga tttctcgaga ctctgttcac
     4141 aaaatcaaat aaaaaagctc tgtgaaataa gtatagttaa caataaaata tccgaacgat
     4201 agagagaatg aactgtgatc tagcagttcc tcgtcgcaaa ttcactagaa ttagcattat
     4261 ttactctaaa ttgttcggat attttttctt gtttttaatt atgattacag tgcctatttt
     4321 taacgaaatt tgcttatttt caaaatgtat gattatatgt tagtaaacac tttcaaatat
     4381 gaaaagagga gctattatgg taggaattag ggatgttgca aaacgggcgg gtgtgtccat
     4441 tgcaacagta tcgcgcgtgc taaatgagga tccgacactt tcagtaaccg atgaaaccaa
     4501 acgaaagatt gcagaatcgg ttaattttta tcattatgtc aaaaaaagtc ccgttgcaaa
     4561 aaatttaaca aatattgcat taataacgac agtttctgaa gtagatgagt tggaagatcc
     4621 ctattttcga acgattcgca gagggattca agtcgaagca gaacaaagca aagtcaattt
     4681 gaagaaaatt attcgtttgt cagaaacacc gttggagcaa gacgatttgc taaaatgtca
     4741 aggtgtgttg attattgggc aggtgctatc ttctgtgatt gaagaagtat cagcaattaa
     4801 ttcgaatgtc gtagtgattg atgaccctag tattgaagct gaagtagatg cagtttatac
     4861 tgatttaaca aaagcaacga atcagcattt agatcgattg tataaaaaag gacaccgaaa
     4921 cattgcgttt atcggaggag gacgtgttcg tttagatagc caaggaaata aacacgtgtc
     4981 agatgatgat tatcgaaaaa tagcctatga gacgtggatg aaacaaaaag gattagaaaa
     5041 atatagtcaa atttatttag aaggctggac cacacttgac ggaatgcagg cagcagattt
     5101 acttttgaaa aaacatgcta aagataagtt gccgactgct atcatggtag gaaatgatcc
     5161 tattgctgtg ggggtttatc gttcacttca aaaaaatggt ctacatattc cagaagatat
     5221 ttcgattgtg agttttgata atattgaagt tgctgaattt ttaacacctg cattaaatac
     5281 ggtgaatgtg aatacagaag aaattggaaa actagcagtt cgcatggtta atgaacgaat
     5341 aaaagaagtt agaaatgttt cgatcaaagt gattgtttct aataatatta ttgttagaga
     5401 aagtgaaaaa aatatcgatt aaaaaagtaa acatccaaaa aagggtgttt acttttttgt
     5461 ttactaatat ttttagtaaa aaatctcgac aaaacattaa gtaaacagtt atggtgttgt
     5521 taacaaaaag aaaacgcatt caaaacagga ggaattaaga tgaaacattt caaaaaaata
     5581 gctttaacag ggatagctgt tttaactggg acattactcg cagcatgtgg tgggggagat
     5641 agtaattcta aagacgggca agtaacttta agctatgcga gttgagatac tgaccaagct
     5701 ccaggactaa gaaaaatgtt agatgaattt gaaaaacaaa atcctgatat caaagtagaa
     5761 atggagacaa cgccttggga tcaatattgg gttaaattag aagctgcaac aacgggaggt
     5821 aatatgccag atgtagtaac catgcactct agcgaatcct ataaatatat gtcacagaat
     5881 gctttgatgg gacttgatga tgttatcaaa gaaaatgata ttgacatggg aaactttact
     5941 gaaggaattg ctgatttcta tacgcttgaa gacgaactat acgcaatccc taaagatgct
     6001 tctgtagtgg ggctatggta caacaaagaa ttatttgata atgcaggtgt cgattatcca
     6061 gatgaaacat ggacatggga aacactcaga caagctgcaa ttgatttaac agatgaatca
     6121 aaaggcatct atggttttgc atcacatatt ggaacagaga ctggctactg gccatttatt
     6181 tatcaaaatg ggggagaagt atttgccgat gacaatacaa aatcaggttt gaatacccca
     6241 gaagcacaag aagctttagc tttttatcga gatattgcac ttgttgatca tgcttcacca
     6301 acaattacag aattacaaga tcctgataag ttaagtcgtt ttcaatcagg tagattagca
     6361 atgatgattg aaggaaactg gtttgcaccg ggatttttga aaaatgacta tctagttgaa
     6421 aatgttgctt ctgccgtgtt acctaaggga aaagttaatg caacaattac aaatggactt
     6481 ggttgggctg ccagtgcgag tacaaaatac ccagaagaag tgaaaaaact aatggcattt
     6541 ttagctagtg aggacgccaa taaaatccaa gcggaaacgg gggcatcaat cccagcacta
     6601 aaaggctttg aagacgagtg ggctgcttct tcagatacaa ttgatttgag tgcttttgga
     6661 aaaatgttgg agtatggagt ccctcgtcca tacaataaag atggattacg agctgaacaa
     6721 gcagaagttc aaattattaa ccgtattctg tctggtgaag taactgttga agatggaacc
     6781 gctcaaattg aaaaagaagt caatgcgata ttgagcggaa attaaattag ttaattatac
     6841 aatatacagg cgaaaggatt ctatttcgtc tgtatattaa tggatgaata ccaaaaaggg
     6901 ggatattatg aaaaatcgaa tttttggaaa atatcgtaaa gatcaagtga tatggggcta
     6961 tatttttatt tttccaacgg cattaggatt aattttatta aatatatggc cagcaattca
     7021 aacagcaatt ttaagttttc aaaaaacggt agggttcggt gatactgaat gggtcggatt
     7081 aagaaattac caaaaaatgt ttgcagacgg tgaagttttt cagtctttat taaacacatt
     7141 aatttatgca gtggtttcga tacctagtat tgttgtatta tccttgttgg tggcagtttt
     7201 aatgaataga aaagttaaag gattgtcgat ttatcggaca atttattttc taccagtcgt
     7261 tgctgcacca tccgcagtcg caatgatttg gcgctggatg ttcaataatg attatggact
     7321 gattaataac atgttggcta aaataggtct cgatggacca gcatggttaa cggatccatc
     7381 gattgcgatt tattctatta tcattgtagg agtttggagt gctattggtt ataacatggt
     7441 gttattacta gccggcttat aagaaattcc taaagattat tatgaagcag cggaaattga
     7501 tggtgctagt ccaatccagc agtttttcaa cattacgtta ccattagtta caccaacatt
     7561 attttttgta gttgtaacaa cagttatcaa tgctttccaa gtatttgatg ttatttttat
     7621 gatgattact cctaatagta ccgccatgtt taaatcacaa tcattagcct atttattcta
     7681 taaacattca tttattttaa atgacaaggg atatggttcg gcaatcgtta tgttcctttt
     7741 agtgattata ttaattatta ctgcaattca aatgaagttg cagaaaaagt gggtaaacta
     7801 ttaaggaggc tatcaaatta tgaaaaataa atttttttca aatgcgccaa tccatatcat
     7861 gcttatttta ggtggtattg caatggcatt accttttatt tggatgattt taacttcagg
     7921 gaaaacgttg gttgaatcta cccaaattcc acctacaatt ttccctgaga aatttcagct
     7981 aagtaactat caacaagtac ttgatttatt accattcatg gctttttata tcaatacatt
     8041 tttaatggtt gttggacgag tgattggctc ggttttattt tcagcaatgg ctgcatatgc
     8101 ttgtgcgcgt ctaagttttc cagggaaaaa tatccttttc agcttagtac ttattcaaat
     8161 gatgattccc ggttccattt ttattactcc acagtattta ttagcacaaa aattgggtat
     8221 tttaaataca attccagcat taatattacc aggggtagtt agtgcgtttg gtacgttttt
     8281 attaagacaa tttttcttag gtttacctga tgaattggag gaagcggcct atttagaagg
     8341 tgccaatatt tggacgatct ttttcaaaat tatgttgccg cttgctaaat caggattggt
     8401 tgcacttgga atattcacag cgttgtttgc atttaaagat ttaatgtggc cattgattgt
     8461 caatacttca attgataaga tgcctttagc tccgggatta gcaaacttac aaggacaata
     8521 tgccacgaac ttcccacaat taatggcagg ttcgttatta gcaatttggc caatagttgt
     8581 gatattcatc gttttccaaa gaaaattcat tgaaggaatt gctacaacag gtagtaaatt
     8641 ataaaactat taattagtga ttggagagta agtacatgac aaaaattaca tttattggtg
     8701 caggaagtac tatttttgca aaaaacgtat taggcgatgc gatgttaact ccaagtctac
     8761 aagatgcaca tattgcattg tacgatattg atgaaaaacg actaaaagaa tcaaatttaa
     8821 tgcttaaaaa tattaataaa aattcgaatg aaaatcgtgc tgatattgtt tcttattatg
     8881 atcgcaaaga agcgttaaga gaggctgatt ttgttatcaa tgctattcaa gtgggtggtt
     8941 ataaaccttc aacagtgatt gattttgata ttcctaaaaa atatgggttg cgacaaacta
     9001 ttggtgacac catggggatt ggtgggattt ttagaaccct acggacacta ccagtaatgt
     9061 tagactttgc taaagaaatg gaagaagttt gtccaaatgc ttggttattg aactatacaa
     9121 atccgatggc gatgttaacc atgggtgttt taaaagcgac gagtattaag actgttggtt
     9181 tatgtcatag cgttcaagta tgcgtaccag aattgtttga gcatttagga atagccgatc
     9241 aatatgactt aaatgacttt caatggaaaa ttgcaggcat taatcatatg gcattcttgt
     9301 tggaaattac aaaaaatggg gaagatttct atccaacaat tcgtcagtta gctgcagaaa
     9361 aaccaaatcc acatccagat tctgtacgtt ttgaattgtt gaaacgtttt ggttactata
     9421 ttacagaatc aagtgaacac aatgcagaat accatccata ctttattaaa tcaaaatacc
     9481 cagaattgat tgacgaatta gggattccgt tggatgaata cttacgtcgt tgtgaacaac
     9541 aaattaatga ttgggaaagc atgcgagagg acattgtaaa taatggggag ttaacacata
     9601 cgagaagccg tgaatatgct tcatatatca tggattctat tacgacagga actccgtcag
     9661 tgattgcagg aaatgtctta aataaaggct taatcacaaa cttaccgaca gatgtttgtg
     9721 tggaagtacc ttgtttagtc gataaaaatg gcgtgcaacc aacctatgtc ggtgaattgc
     9781 caacacaatt agctgcttta aatcggacga atatcaatgt tcaagaactg acagtagaag
     9841 ccgctttaac actagataaa tcaaaaattt atcaagcagc gtatttagat ccacatctat
     9901 catctgaatt gtctttaaca gatatcaaaa atttagtgga tgatttaatc gaagcacatg
     9961 gggactattt acctgaatat aattaaaaag aagctgaaag agtaaggaat tttcaattcc
    10021 ttactctttt cttagttaaa tttagtaaaa atattgacaa aacacttagt aaacagttac
    10081 attagtgtta acaaaaagaa aacgcattca aattgtataa ggaggagcat aaaatgaagt
    10141 ggtctaaaaa aattatttta gcttgtatag cactaggagc aacgatctct ttgactgctt
    10201 gtggaaaaaa tcaggaagat gcaagcggta aggtagaaat agagtttttt aatcaaaaaa
    10261 aagaaatgac tcaaacgatt caggagattg ctaaagattt tgaagcaaaa aaccctgata
    10321 ttcatgtaaa agttgtggat gtaccgaatg ctggtgaagt aattaaaaca cgtatgcttg
    10381 caggcgatgt accagatgtc attaatcttt atcctcaaag tatagaattg aaagaatggg
    10441 cgaaagctgg ttatttagag gacttaaccg aagaaccgta tttagagaat atcaaaaatg
    10501 gatatgcaca acgttttgct attgaggatc gtgtatatag cataccatta accgctaatg
    10561 tctatggttt ttactataac aaaactgctt ttgaggaaat ggggattcaa gcaccagaaa
    10621 cttgggcaga atttgaaaaa atagtagctg agattaaaga tcaaaacaaa gtaccgtttg
    10681 cgattgcagg agcagaaggc tggactttaa atggttatca ccaactagct atagcgactg
    10741 ctgctggtgg tgaagaggaa gcgaacaatg tttggcgttt ctcagaagtt aatggaatta
    10801 atgctgaaag caaagaaatg caaaaagatt ttaatcgatt agatttgtta agagaaccaa
    10861 aagctttgca aaacaactgg caaggtgccg gttataacga tacagtagtt acttttacaa
    10921 aaggtgatgc gttgattatg ccgaatggtt catgggcaat gccgatgatt cattcacaaa
    10981 atccagattt tgaagtagga acttttcctt ttccagcaga tgaagcaggt caaagtctaa
    11041 cgattggcgc aggagaccta gccttatcag tttctgcaac ttctcaacat aaagaagaag
    11101 ccaaacgttt tgtagaatat atgacaacac ctgaagcgat gcagaaatat tatgatgtag
    11161 atggtgcacc gtgtgctgtt gagggcgtta tcgagaatac tcaggattca ccattaagtg
    11221 gtttaactga acttgctttt actgatcgtc atttagtttg gttagccaaa gattggaata
    11281 gtgaaaatga tttttatacc ttaacaacaa attatttaca caatggcaat caaaaagcca
    11341 tgattaacgc attaaatgct ttctttaatc caatgaaagc tgacggaagg taggaaaata
    11401 aaatgaaaat aaaagaaatt ttaaatcgtt actgggcaat catttttgta gcaattccaa
    11461 ttattttgca gatgattttt ttctactttc caatggttca aggtgctttt tacagtttaa
    11521 ctaattggac tggtctgact tataattttg attttgtggg tttgaacaat tataagattc
    11581 ttttttctga tccgaagttt atcaaatcga ttggcttcac tttaattgtg acgctttgca
    11641 tgattgctgg acaaattggt tttggtattt tgattgcgcg ggcgttgaac gcaaatatta
    11701 aagggaaaac attctttaga gcatggttct tttttccagc ggtcttatcg ggcgtggttg
    11761 tttctttagt atttaaacaa atttttaact atggtttacc aaaaattggt agtatgttag
    11821 gcattgaatt tttacaaacc agtcttttag gaacaacatg gggagcgatt tttgcagtaa
    11881 tttttgtttt actttggcaa ggaattgcca tgccagtcat cattttctta gcgggttttc
    11941 aaagtattcc acaagaaatt attgaagctg ctagaatgga tggggcgaat gataagcaaa
    12001 ttttttggaa tattgaaatc acctacttat taccaagtat ttcaatggta tttattatgg
    12061 cattaaaagg tggcttaaca gcatttgatc aaatttttgc tttaactggt ggtggaccaa
    12121 gcgatgccac aacatctttg gggttacttg tttataatta tgcttttaaa aacaatcagt
    12181 ttggttatgc gaatgctatt gctttagtct tatttatctt aattgggatt gtttcactgg
    12241 cacaattaaa attatcgaaa aaatttgaaa tctaggaggt ttcccaatga aaacatcaaa
    12301 agaaaaaaat atttctaaat acttgctttt attattaggg ggcttcatga ttttagttcc
    12361 cttagtttca acgctattcg gttcatttaa aacaacaccc gatattatga ataatttctt
    12421 tcgttttcct aatccagcga ctttggaaaa tttccgaaga ttgttagcgg atggaattgg
    12481 tcattacttt tggaattcgg caatcatcac aatcattgca gtagttgctg tgacagcaat
    12541 tattcctatg gctgcttatt cactagcaag aaaaatgact cgtgggaaag catacggatt
    12601 gatgtattta tttttaattt taggaatttt tgttcctttc caagtaatta tgattccgat
    12661 taccgttatg atgagtaaaa ttggtttagc aaatatgtgg ggattaatct tactctatct
    12721 cacttatgcc gtaccacaga cactattttt atatgttggt tttattaaac taaatgtgcc
    12781 tatcagtctg gatgaggctg ctgaaattga tggggctagt cactttacaa cttatttcaa
    12841 aattgttttc ccaatgttaa aaccaatgca tgcgacgact attattctta atgctctatg
    12901 gttttggaat gatttcatgc taccattatt aattttaaat cgtgattcta atatgtggac
    12961 actaccattg tttcaatata attatacggg acaatatttt aacgactatg gtccaagttt
    13021 tgcttcctat acagtaggaa ttgtcacgat tacactagtt tatcttgctt tccaaagaag
    13081 tattatagct ggaatgagca atggagcagt gaaataacca ataaattatt ctcattataa
    13141 aggagttcat aaacttatga ttatatttga caatcacaca caaacatttc atttacaaaa
    13201 taaaaaaatt agctatctat taggaatcga agaaaataat tatttaacgc atctttattt
    13261 tggaaaaaag attacgcact attctggtgg gtatcgctac ccccgaacag atcgttcctt
    13321 ctcacccaac aatgcaaatg cgcaagatcg tttattatct ttagacacat taccattaga
    13381 atttccagga tatggacatg gtgattttag agaaccggcg cataatatta aattaggaaa
    13441 cggtagtcgt gtaaatgatt ttcgttatga tagctacgaa attcaaaaag gaaagcccag
    13501 cttagcggga ttgccagcct tatatacgga agatgatgat gaagctgaaa cgctgattat
    13561 tcaattgaaa gacgcgatta gtggattgac tttacagttg ttctacacaa tttatcaaaa
    13621 tcacagtgta attattcgtt ctacgcaatt agtaaataat agtgatgaaa cagtggagat
    13681 taaccgctta gctagtcaat caattgattt tccaaatcgt ccactcgagt taattcattt
    13741 aaatggtacc tgggcaagag aacgtcaaat gactagagaa aaaattcata cagggataaa
    13801 agtattagat agtaaacgcg gatcaagcag ccatcaacaa aatccgtttg ttgctttagt
    13861 agatgccaaa acagatgaat ttcaaggaga ggcatatgga tttaatttag tttatagcgg
    13921 taatcacgaa acggtgattc aaaaagatcc atttgaccaa acacgggtca ttatgggaat
    13981 taattcattt aattttggtt ggcaactaaa aactggcgac tcatttcaaa cgccagaagt
    14041 cgtgatggtt tatagtaatg aaggtctgaa tacgttatca cagacgtatc atgaattgta
    14101 taatcaccgt ttaattcgtg gaaactatcg tttaaaagaa cgtcccacgt taatcaacaa
    14161 ctgggaagca acgtattttg attttaacga agaaaaaatc atgaatattg tcgatgatgc
    14221 gaagaacttg ggcattgaaa tgtttgtttt agatgatggt tggtttggcg gtcgtgaaaa
    14281 tgacttcact tcattaggtg attgggttga aacagaaggc aaacttgctt gtggtttgga
    14341 aaatttagcc aagcaagtgc atgataacgg gatgaaattt gggatttggt ttgaaccgga
    14401 aatgatttct gaagatagtg aactatttcg agcacatcca gattgggcat tgggtattcc
    14461 aggtcgtgac cgctcaatta gtcgaaatca atatgttctt gatttttcaa gacaagatgt
    14521 tcgtgataat atctatcaac aaatgacggc gatttttgat cgtgtaccga ttgattatgt
    14581 gaagtgggat atgaatcgaa atatgacaga agtctattct gtcttgttgg actcaacgat
    14641 gcaaggtgaa gtggctcatc gttatatgtt agggttatat gaatttttag aaaagttaac
    14701 aacgaattat ccaactattt tatttgaaag ttgttcgggt ggaggcggtc gttttgatgc
    14761 aggtttcttg tattacatgc cacaaacttg gacgagcgac aataccgatg ccgtggctcg
    14821 tctgaaaatt caatatggaa ccagcttagt gtatccaatt tcaagtatgg gtgcgcatgt
    14881 ttcagcagtc ccaaatcatc aaacaggaag agaaacgagt ttaactattc gtggcaatgc
    14941 tgcgatggct ggtgtttttg gttatgagct agatctaacg caattaactt ctgaagaaaa
    15001 agcagaaatt aaagaacaag ttgcctttta taaaaagcat cgccaacttt tgcaatatgg
    15061 aactttttat cgcttggaaa gtccgtttga aggcaatgat actgcatgga tgtttgtctc
    15121 aaaagaccaa acagaagcca tggtctttta tttccgagta ttagcagaag ctgcgcatcc
    15181 attagtcaca ttgaaattag caggattagc agaagaagca acttatcaaa tggaagatgt
    15241 aacatttgct ggcgatgaat tgatgaatct cggtttttat attgactcag aactacatgg
    15301 tgattatgcg acacaacgct tttatttgaa acagatatag tagcttgaat ttacaatata
    15361 agtgcaacac caaaagagac tcattccaaa cgaagaaaat atgttttatt tacacgcatt
    15421 tactaaaaag cagcctgatt tgaattggga aaatgaagaa gtacgtgaag aaatctatgc
    15481 aatgattaac tattggttag ataaaggact aggcggattt agaatcgatg cgatattaaa
    15541 catcaaaaaa aaactagagt atggcatctt tgaatctgat ggagaagatg ggttagcctt
    15601 cattgggccc tggattctta atcagtcggg cattgaagtg tggttaaaag aaatgagaga
    15661 gcggacattt aaaccacata atagtatgac ggtagcagaa gccgatgttc ctaatgaacg
    15721 tttagatgaa tatattggtg aagatggcta ttattcgatg gtttttgatt ttagttatac
    15781 ggatattgat gtacctgaaa ctggtgaatg gtttaaagat agccaatgga catggacgga
    15841 tatgcgtacc aatattttta cgaatcaatt agttacgcaa gataaaggtt ggggcgcctt
    15901 atatttagaa aaccacgatc aaccacgttc aattaataaa tatattccag aagaatggat
    15961 caatgattat agtaaaaaaa tgttggcgac gctttttatg ttactaagag gtaccccttt
    16021 tatttatcaa ggacaagaac taggaatgac caacatagaa atggcatcat tagaagattt
    16081 tgatgatgta gctacgcatt ctcagtataa acgggcatta gaatatggat tggcaccaga
    16141 gcaagcattg aaagctgtca ataaacgaag tcgggataat tctcgcacac ctatgcaatg
    16201 gaatacgcaa aaaaatgcca gattctcaac ttctgataat gtatggttaa aagtaaatcc
    16261 taattatcca gaactcaatg cagaagctca gttaaccaat caacagtcgg tattcaatta
    16321 ttatcatcaa ttaattaaat taagaaaaaa tagtgtatac aaagacgtgc taatctacgg
    16381 acaatttcta ccagtgaaag ataaaaatga acatattttg ctttatgaac gccaattaga
    16441 agataaaatc gtattagtta tcttaaacgt tacagcaaca gaacaagaat attatgtaga
    16501 tgaacagtac caacaagtat taatcaataa ttatgaagat gttttattag atcaaagtcg
    16561 aaaattgcgt ttacgaccgt ttgaaagtat tgtattagct aattatggag gacaattatg
    16621 aaaattaaaa acgaagcaat gctcatcacc tatgcagata gtttaggaaa aaacatgggc
    16681 gaattaaaac aagtcttaga caaacattta caaggtgtta ttggtggtgt acatctgttg
    16741 cctttttttc catcgacagg tgatcgaggc tttgcgccga gtgattatac gacagttgat
    16801 ccagctcttg gaacatggga agatgtggaa gcattaggtg aagattatta tttgatgttt
    16861 gattttatga ttaatcacat ttctcgtgaa tcaaaatttt tccaagattt caaaaaaaac
    16921 catgagcagt caccatacaa agaaatgttt atccgtattc atgaattttt cccagaaaat
    16981 cgaccaacac aagaagatat tgatttaatc tacaagcgta aagacaaagc gccgtttcaa
    17041 acggtagaat ttgcggatgg tacgacagaa gaggtttgga atacctttgg ggaagaacaa
    17101 attgatttag atgtgaccaa agaagttgtg aaagaattta ttcgagaaac catcaaagat
    17161 atggcgaacc atggctgttc tttaattcga ttagatgcgt ttgcttatgc gattaaaaaa
    17221 ttagatacca atgatttctt cgtggaacca gatatttggg aacttttaga cgaagtgcgt
    17281 caagaagcag cgaaatatga cgtcgaatta ttaccagaaa tccatgagca ttattccatt
    17341 caaatgaaaa ttgccaatca tgattattat atctatgact tcgccttgcc aatggtcact
    17401 ctgtattcac tttatagtgg aaaatcagaa cgtttagcca attggttgaa aatgagtccg
    17461 atgaagcagt ttacaacatt ggatacgcat gatggaattg gtgttgtgga cgctcgtgat
    17521 ttattaacag atgaagaatt ggattacacc tctgaggaat tgtataaagt cggggcgaat
    17581 gtgaagaagg tctattcaag tgccaattac aataatttag atatttatca aattaacagc
    17641 acctattata gtgcattagg cgataatgac cgtagctatc tgttagcacg tgcgattcaa
    17701 gtatttgcgc cagggattcc acaaatttat tacgttgggt tattagcagg aaaaaatgat
    17761 attgaactgt tggaagcaac gaaagaaggg cgtaatatta atcgtcatta ctatgattta
    17821 gccgaaattg aagaagaagt gcaacggcca gtgattcaac aattatttgc acttttaaaa
    17881 tttagaaatg aatctgcggc gtttgatttg gatggaacga ttgaagtagc aacgccatca
    17941 gaatcagaaa tagtgattac acgtaaaaat aaaaacggta gtgatagtgc aacattaacg
    18001 gctaacttgg atacgaaaga atttatcatt gagtctaaag atataaaaat tttcactagt
    18061 gttgcattaa aattttaacc tattccatag aacaaaaaat cctttatact agcacttgga
    18121 tgacttatcc aagaccaata aaaaggactc atacatggat aagtataaaa caaatacagc
    18181 atttaataaa tggttttctg ccataaaatt aggaaaactt tctgtcaact cccaaaaatg
    18241 tatttttgat tacaattcgt atcgtaaaaa actgtctttt gaagctgtgc tcaagctctt
    18301 tctctacgca gttaatgaag aaaaagaaag tttgcgtgat ttgagtacag ctcttatcga
    18361 ccgatcgctc caactagaaa ccgatgtgtc atcgattagt catacccaat tgtctcgagc
    18421 atttaatgca cttgaaccta aagtgttaga agaaattttt caacaactgt tagaaaaagt
    18481 tcaacatcaa atcaaaccaa ccaagcgaaa tcatctgtat ctgattgatt ccagtacgtt
    18541 ttcgcttagt ttaaaacgtc ataagtgggc gactttccgt caaacaaaat caggtgtcaa
    18601 gttacactta aactattgtt atatggacga tactacgatg tatccaactg attttacctt
    18661 aacaaatgcc catgaacatg atctcaatca actaccagta ttggtgaatc aaccagaggc
    18721 aacttacgtg tttgatcgtg ggtattagga ctttgaaaag atggatcaga tgcattggga
    18781 tggctatttc ttcgtcactc ggattaaaaa aaacaccaaa gttcacgtga tagatactct
    18841 ggaaacatcg ccagaaacag aaattctacg agacgagctg gttcgtttag gatctaaaac
    18901 ctatctaacc gccaatttcc gactagtcac tgttcaagat aagaacggac gtgtgtttca
    18961 atttatcacc aatcgaatgg acgtctcttc aaaagaaatc tctgatatgt atcacgctcg
    19021 ttggcaaatc gaattgtttt tcaagcacat caaacaacac atgaccatta aaaccttctt
    19081 ttctcaaagt gaaaaaggcg tgcagaatca attgatttta acgatgattt ccgctctcct
    19141 cacgtttctc atcaaattgg agacgaaaac ggaaaaatca gtgttccaaa tcaaacgatt
    19201 ctttcgttat ttactgtttc agccctttga atgttgtcta gaaaaattaa ttcccacatg
    19261 agaaaaaaga aatttggctc gttacttttg gttttcagtt gaataacaga taaaaaattt
    19321 tctggaaaaa gtcatcctct gcgtcagcac agtttttttt gacttatttt cagaggaaat
    19381 agttttcaga atattctaaa atgttttatg caacactagt gatatttttt agaattagat
    19441 ttctaatgtg cattttttga gtattaagga gcaagtatac aaagatttat aaagctgatt
    19501 aaatttgaat aaaaatatgc aggaacgcta ctatttttag tagtgttctt ttttatattt
    19561 gaataaaaaa tgtggtggtt aaatggtggt tataacaaaa aatgaattaa tgaaattagg
    19621 ttatgggaag tatcagtctg agagaatcat acgacaggca aaagcaatta tggtttcaaa
    19681 aagctatacc tattacgaaa atagaagatt aggactagta cctgttgaat ctgtagaaga
    19741 agttttaggt atgaagatag aggtagagga ggattttcag aatcatggtt aattttaaaa
    19801 aagataaaaa tgggaaatat cattttactg ctagtttggg attcgacaaa cgaacaggga
    19861 aaagagtaca aaaaaacgta gaggggttaa aacagttaaa gaagaaaaaa agcgtatacc
    19921 gaacttgttt ctaattatgg aaaagaagca gaaacttctc aaagtactat gttgtttgag
    19981 gagttttttt ataaattatt cctaccttat tatcaaggga aagtagaaga aagaacatat
    20041 aaaaatcggt tatctacaat caaaaaaact tttcttattt ctttaaaatg gagttaagag
    20101 aaatacgtcc aattgacatt caaaagtggc aaaataattt attgagcaaa tataaaaatt
    20161 cttatgtaag aaacatgtat gggatttttc agatgtcatt agatcgagca gttgttttgg
    20221 gactacttga aaaaaatcct gcaaaagtta ttggaaatgt gaagaaaagg aaaactacag
    20281 ttgatttttg gacaaaagaa gaatttacaa aagttatttc aactttttat attgatgatt
    20341 tgtaccaaca ttttggtttt atttgtatgt ggttactttt tatgaccgtt atgcgtttgg
    20401 gagaagccac tgcattaact tggaaagata ttgattttga aaataaaaac ttgcgtgtga
    20461 ataaaacact ctattataag agagccaaaa cctatgagtt tgtagaaccc gaaacggctt
    20521 ctagtattcg tacaatcgaa ctagacgaca atacaattac ctttttaaaa gcttggaagg
    20581 agcgtcaagt ttcaatagga ggcatgtcgt ttgtactttc ttataatgga acacctactc
    20641 agaaatatac aatccgtcat attatgagaa gacatgcaac gatcgttggt gttcatataa
    20701 tccgcataca cggattaagg catagtcatg cttctttgct tatatcacaa taaaaagatg
    20761 cacttcgaat taaagaaagg ttaaaacatg ctgatgtaca aacgacttta ggaacttatg
    20821 gtcatttgta tctaaattca ttgagagaga taacaaatga tcccacagat tatatccaaa
    20881 ctaatttttc taaaatcaaa ccgacaccta tttacgataa taaaaaataa atgataaaca
    20941 aaagtaggat cacaccaaaa gaaacacact agaaaaacta taatacaggc ttttctagcg
    21001 tgttgtttct tatttccgcc tatttataca ttatactcat aaagcattta atgcatcgat
    21061 cgtagccgca ttgtctatat gggtatataa agttgttgta ttggtacttg aatgtcccaa
    21121 ttgttgggca gttaatacca atgaatttgt ctgttggtag agtctagtag ctaatgtatg
    21181 acgcagtttg tgagggctga cttgaattct aaagcctttt gaatattttg ctaccatccg
    21241 ttcgattgca ataccggata tacgcttggc ttctccttgg taaattgcta gaaataaagc
    21301 tttttcgttt tctgttactt tatatcgttc tttccgaata gctagataat tagctaaata
    21361 ttccattgcg aatgctgcta tattgacagc atctctttta tttccttttc tagttactac
    21421 cacattattc ttatctaaag ataggtcatc taaattaata tttataacct cggagagtct
    21481 caatccagat cctaaaatca gtgcattgat cgccaggtct ctttctacat ctcgttgata
    21541 atagatcaaa gctcgtttac tcaaagtatt tttatattca ttggcaatat aatctagata
    21601 attttgtgta tccttatcta gaaataattt tttctcaatg gcattggcac gtgcagctaa
    21661 cgtagtttta tctgtgatgg ttgctacttg atccatagga ttattgtcta agtatggttt
    21721 tcctagagga ttattggaac ttttcgaaag atatttaaat agaaccttta atgccgtgat
    21781 atttctattg atagtagaag tagatagacc taagttatta ttatctttga gcttagtttt
    21841 tgttgtctct ttttttacat tttcttttgt gcgacgtttc aaatgagatt tgtacaagtt
    21901 taggtcatag gcagttaact gtcttatcga ggtgatagaa gtatcaggaa cgatagactc
    21961 actcagaagc cattcataaa agagcttata attttttaga tattctaaag tggttcgagg
    22021 tgattgatct ttatcctcac gatcttgaac aaaatctttt atataatcgg gcatatgaag
    22081 tatgattgta tctatttttt gatatatttc tgtatttttc atgtcattca tcctttcaat
    22141 aagtagggga gaagagtatc tctataattg gactatacca attatagaat gttttgtcaa
    22201 ttggtatgct ctaaaattta ttaatttgtt tcaaaaaaag catataaaca ggtatacgtt
    22261 taataattta aaataaatgg tatacataaa tagggaagga aatagaaggg agcaatctaa
    22321 attttttgaa ttttcttaat taattatata gaaagaacaa tctttctata gagttagaca
    22381 tgagagggac tatgcttgat ttaaaatcaa caagcgtata tactgtatat acaaagaaaa
    22441 aggagggaga agactatggc aattaaagaa agaaaattta gggaagtggg gaactcagta
    22501 gtattaacta tttcaaagga gtttcttaaa gaaaatgggt taaatcccgg agatacggtt
    22561 tttttagatg aagaaaaact tcgagcggca attacaaaaa aacaagcctt atctgatgaa
    22621 attgatttag caatatctca agcattaact gaatatgata ctacttttag agagcttgtc
    22681 gataaatgag tattcattac ctagaagcca aagatattat aaaaatgaat gtaatccaaa
    22741 taaaaaaata ctctcctaat gaaccccttg gtgtaaagga tgcgaatgct ttagaaatgt
    22801 gtgtagggca acttcgagcc tcagcttttg gtgaagaggt ttatccttcg atttatgaaa
    22861 aagcagctat cctacttata caattgataa agaaacatcc ttttcacaat gcaaataaac
    22921 gtactgcttt cttagctact tttgtctttt tgaaaatcaa tggacagctt ttagctattg
    22981 atcaaaaaga tgctgtaaat ttagcggtat atattgctac gtatgataag gatttcgatc
    23041 aactaaaata tgaagtgatc aacactataa agacacatac gcagcccatt taaggagtta
    23101 ctgtatgtgt ctttataggg ttattagctg ctatagcgaa ataattgaag taaattacaa
    23161 atagctataa ttcaaatcca actatataga aaacgtaaat tttctatata gttggattaa
    23221 gataacgact atttatttta tcatattttt actataccct atacttttac ttggtatatt
    23281 tatgaataat cgcaaattta cccctatact actagtttgt aagttagatt aataaaaata
    23341 ctaaagttat ttgttctttt attaaataca cctacaactt ataaattgat ggtatttaga
    23401 taacacgaaa gaataaaaat aacagctgat agtaaagttt gttgacgatt gctactcaat
    23461 cgaaagtgag ttagtgcgta tgaacttgct tctgaaaact tatgaaatga aagacctttt
    23521 acatcttgca gaaatcggta ctgggattaa actaagcaat aaaaactaat gttcccctca
    23581 ttctttgaac ttctactatt tgatttattt attctgataa atgacgattt caccttcagg
    23641 atcttcaaat cccgtataga atttatttaa gatctcttca gtatcagtaa taggataggc
    23701 gataggccac ataccctttg atggttagaa taaactgctc aaaatctgta tcgtatgttg
    23761 gctttagcat attaacaaag gctatctcca aattataaac cataagcgtt gaatcgttct
    23821 taacaatttc aattcttcct aacccaaagt cagaagatat aattaccgga cttgccttac
    23881 ccttcatgtg ggttgttgag attccatatt taaagggaat tgtcggtact atcggcggtc
    23941 tgaaaaacat cccaaaaata aaaaaatctt tcccctgcta gtctcctaag tccctttgta
    24001 gcggatatgg caccataatc tcattttatc gaagtgagtc atggtttatc tgttggccaa
    24061 ccaaaattga tacaatctaa cgatgcaccc tctacggcat tctaggggag tgcacttgca
    24121 ttcacccaat aaaaaccggc agttagcaaa gaacttatgt agtcgtatgt tcttactgtt
    24181 aacatacaag aggtctcctt tccacaattg cttagcagta aacgggaatt tacaaagcta
    24241 atctcttcca ttttcttatc tttgttctaa aagtcccaaa tggagcaact gtatttacat
    24301 gaataaattt atatacttcc catacagccg ttttggttgc ctcgtctgcc cattttcttt
    24361 tgtgtgcagt gaatagatct tcatcactca ttgaatcaat catcatgtta atatcatcaa
    24421 tattacgatt gagaatttcc tctaattctt tcaatggtag atgtgcatag gtatttgtaa
    24481 accacttata taactctcct aactgattcc atttaaattg atcagaaggc gttttaacag
    24541 ataatccatt tctttcatca ttttcccatt ttagaattaa attcgtccaa ccaacttgat
    24601 atgctaaatt ttcagcaggt gttctatcca cttcctcaac tcttttatcc ttcaattctt
    24661 caggaatagt ttcaaattct aaaatatatt tagtataagt cctatttatt tcttttttta
    24721 attcttcttt atttttatat gttctcatta tattacctcc ataaattcct aattgtctcc
    24781 tttaattcat ctataatttc atccacgata ttttaatccg ttctacaatg catatctgat
    24841 atgcattgac tgtctaactc atatgtgaat gatttataaa atatcccaac gtctcatcta
    24901 aagaaatgtt gatcgtcagc aaggagctaa aaaatacgaa tatatttctt ttgtagtaaa
    24961 atcggactgg tttgcatatc attttcttcg taaatatcaa aatcatgtta actactatca
    25021 attactaata aaaactacca ctttaagcgt taagaactgt taagtttagt taagtgagga
    25081 tagttcacaa aatccattgt taaacttcac gaaagtggta aaactcaaaa tgctttatat
    25141 aggattacag catcgctctt tcttcgattg cgcgttgggt aaaaaatatt atgaagtaaa
    25201 aattgacgat gacacgttcc ttacggctca tcagatgaaa cagctacaaa aacgaaatga
    25261 tcaactcgaa gaaaatttaa ttctaaaaaa gcgattgcca tattcacgcc tcgctcgacg
    25321 aactattaaa ggtcaagatt tacctgaaga tcatcctttt taggaaattg aagcttttta
    25381 tgttgcacaa tgcatttata atactacaat gatgttttca ccagatattc ttctagggtg
    25441 cggtgtaatg aaacagatac acttgcaaaa gaaaattcac cgcgaattta ctaaaattat
    25501 gaataattat gttaatcttc cgaaattttt ttccaaaatt aagggatcaa gctggtgtta
    25561 tagattgttt aaggttggca aaaaaataac ttctttttaa acaattatag aaaaccgata
    25621 ctctgttaag ttggttgaca cggtgtcgat ttttatgact ttcttattct gaaacttcac
    25681 taacggacag tacctaaaaa gtctatagta tggacgttta cgagtactat tttatcaaaa
    25741 atgctgctca tttcaactta tatgttttag tgaactaccc ccactta
//
LOCUS       DACDXE010000038        25194 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.038, whole genome shotgun sequence.
ACCESSION   DACDXE010000038 DACDXE010000000
VERSION     DACDXE010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.038"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..746
                     /locus_tag="IWM23_001580"
     CDS             99..746
                     /locus_tag="IWM23_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9884220.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1139..3784
                     /locus_tag="IWM23_001581"
     CDS             1139..3784
                     /locus_tag="IWM23_001581"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAP9884221.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="IWM23_001582"
     CDS             4094..5407
                     /locus_tag="IWM23_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAP9884222.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="IWM23_001583"
     CDS             5397..6050
                     /locus_tag="IWM23_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9884223.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="IWM23_001584"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="IWM23_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="IWM23_001585"
     CDS             6787..7872
                     /locus_tag="IWM23_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9884224.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="IWM23_001586"
     CDS             7869..9761
                     /locus_tag="IWM23_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9884225.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="IWM23_001587"
     CDS             9768..10145
                     /locus_tag="IWM23_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9884226.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="IWM23_001588"
     CDS             10299..11081
                     /locus_tag="IWM23_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9884227.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="IWM23_001589"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="IWM23_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9884228.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12083)
                     /locus_tag="IWM23_001590"
                     /pseudo
     CDS             complement(11996..12083)
                     /locus_tag="IWM23_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            12447..13109
                     /locus_tag="IWM23_001591"
     CDS             12447..13109
                     /locus_tag="IWM23_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9884229.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="IWM23_001592"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="IWM23_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="IWM23_001593"
     CDS             13637..13837
                     /locus_tag="IWM23_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9884230.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="IWM23_001594"
     CDS             complement(14091..15770)
                     /locus_tag="IWM23_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9884231.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="IWM23_001595"
     CDS             complement(15772..15984)
                     /locus_tag="IWM23_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAP9884232.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="IWM23_001596"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="IWM23_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAP9884233.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="IWM23_001597"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="IWM23_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAP9884234.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="IWM23_001598"
     CDS             19959..20156
                     /locus_tag="IWM23_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884235.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="IWM23_001599"
     CDS             20309..20794
                     /locus_tag="IWM23_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAP9884236.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="IWM23_001600"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="IWM23_001600"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAP9884237.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="IWM23_001601"
     CDS             21671..22198
                     /locus_tag="IWM23_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAP9884238.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="IWM23_001602"
     CDS             22289..23026
                     /locus_tag="IWM23_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9884239.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="IWM23_001603"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="IWM23_001603"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAP9884240.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="IWM23_001604"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="IWM23_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAP9884241.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DACDXE010000039        24873 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.039, whole genome shotgun sequence.
ACCESSION   DACDXE010000039 DACDXE010000000
VERSION     DACDXE010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.039"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..908)
                     /locus_tag="IWM23_001605"
     CDS             complement(138..908)
                     /locus_tag="IWM23_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884242.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(901..1836)
                     /locus_tag="IWM23_001606"
     CDS             complement(901..1836)
                     /locus_tag="IWM23_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884243.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(2152..3801)
                     /locus_tag="IWM23_001607"
     CDS             complement(2152..3801)
                     /locus_tag="IWM23_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9884244.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(3918..6521)
                     /locus_tag="IWM23_001608"
     CDS             complement(3918..6521)
                     /locus_tag="IWM23_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9884245.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(6533..7582)
                     /locus_tag="IWM23_001609"
     CDS             complement(6533..7582)
                     /locus_tag="IWM23_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HAP9884246.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(7608..7922)
                     /locus_tag="IWM23_001610"
     CDS             complement(7608..7922)
                     /locus_tag="IWM23_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9884247.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(7938..8384)
                     /locus_tag="IWM23_001611"
     CDS             complement(7938..8384)
                     /locus_tag="IWM23_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAP9884248.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(8385..10361)
                     /locus_tag="IWM23_001612"
     CDS             complement(8385..10361)
                     /locus_tag="IWM23_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAP9884249.1"
                     /translation="MKIEIIIVHLSCIQEVKIMGIEFSERQMKILYFLIENEHTTYKE
                     LSEQLYVSTKTISKDILILKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKIN
                     IADSPEDRLTYVFAKLLNTNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLV
                     SDRNGVRIKADEKSKRRLMSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDI
                     LKKVAELLNRYMNKTQLKFTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNT
                     LILIKMIEKAFSIIVPIDEQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVR
                     IKLNEFYPDEELINNLVLHLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEE
                     IEERFCIHFNEDEIAYVTLHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFA
                     DMVEIEDVLSINELQDRKVTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQL
                     NEQTEDAFLNLLDKRFCFFTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKL
                     SSTAMNDFAMPHIEVDFIKKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKI
                     YETFNEIASNHELLANLKTSSSFEKLVYILKEGL"
     gene            complement(10519..11415)
                     /locus_tag="IWM23_001613"
     CDS             complement(10519..11415)
                     /locus_tag="IWM23_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9884250.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(11408..12088)
                     /locus_tag="IWM23_001614"
     CDS             complement(11408..12088)
                     /locus_tag="IWM23_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884251.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(12089..12871)
                     /locus_tag="IWM23_001615"
     CDS             complement(12089..12871)
                     /locus_tag="IWM23_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9884252.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(12864..13781)
                     /locus_tag="IWM23_001616"
     CDS             complement(12864..13781)
                     /locus_tag="IWM23_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9884253.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(13945..14268)
                     /locus_tag="IWM23_001617"
     CDS             complement(13945..14268)
                     /locus_tag="IWM23_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9884254.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            15056..18283
                     /gene="fss3"
                     /locus_tag="IWM23_001618"
     CDS             15056..18283
                     /gene="fss3"
                     /locus_tag="IWM23_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAP9884255.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18396..18710
                     /locus_tag="IWM23_001619"
     CDS             18396..18710
                     /locus_tag="IWM23_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9884256.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18723..19097
                     /locus_tag="IWM23_001620"
     CDS             18723..19097
                     /locus_tag="IWM23_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9884257.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            19107..19442
                     /locus_tag="IWM23_001621"
     CDS             19107..19442
                     /locus_tag="IWM23_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9884258.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            19525..20865
                     /locus_tag="IWM23_001622"
     CDS             19525..20865
                     /locus_tag="IWM23_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9884259.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            20943..21617
                     /locus_tag="IWM23_001623"
     CDS             20943..21617
                     /locus_tag="IWM23_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9884260.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            21850..22284
                     /gene="dcm"
                     /locus_tag="IWM23_001624"
     CDS             21850..22284
                     /gene="dcm"
                     /locus_tag="IWM23_001624"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9884261.1"
                     /translation="MRFLDLFAGIGGFRLGMEQASHHCIGFCEIDKFARRSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQRFSDTRGTLFFEIARF
                     TTLLRPKLLFLENVRGLLNHEGGATFETILRTLDGLGYDVEW"
     gene            22285..22992
                     /locus_tag="IWM23_001625"
     CDS             22285..22992
                     /locus_tag="IWM23_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAP9884262.1"
                     /translation="MLNSKAYIPQSRERIFLIGHSRDACTEQVFPIIGPSSTSDQNIR
                     NLVNINPSNRGMGGQVYGSDGVAPTLTGDEGIKIALPARDGISVAGLLPGKFEQGNRV
                     YEVTGTAPTLSTKQGGTKIMIRKNETEYQEVKPEDSVNLAFPNSTSRRGRLGKQSVHT
                     LLTGDQQAIVTDQYQIRKLTPRECWRLQGFPDWAFDRDSQVNSDSQLYKQAGNSVTVP
                     VIFDIARRLKELDVHGL"
     gene            22982..23272
                     /locus_tag="IWM23_001626"
     CDS             22982..23272
                     /locus_tag="IWM23_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884263.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            23531..24715
                     /locus_tag="IWM23_001627"
     CDS             23531..24715
                     /locus_tag="IWM23_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9884264.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            24730..24849
                     /locus_tag="IWM23_001628"
     CDS             24730..24849
                     /locus_tag="IWM23_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9884265.1"
                     /translation="MGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKGK"
BASE COUNT         7738 a         4794 c         4640 g         7701 t
ORIGIN      
        1 ttcacaacct ctccactaaa cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa
       61 cgacaaaata tgaggaacta ttttcccgtt tgtttctcta atactcaggt caaaacgctg
      121 atttcacaac ctcttttcta acttctcaat cctcttcctt tattgatcaa ataccaagta
      181 acggcgtaca aaacaacgat gaatagcaca agtacgatca tcgatgttgc tactggcaca
      241 tcgattgttc caaggaatcc gtaacggaaa ccggagatca tataaacgat cggattgact
      301 ttcgaaacag cttgccaaaa tggcggcaac atagagatgg catagaaaac gccacctaga
      361 taagttaacg gctgcaagac aaatgtaggc acgatcgata catcgtcata agactgagca
      421 aaaatcccgt tgagcagtcc tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg
      481 atgaccatga accatgaata cacatgaagt ggtacaaaga acaaggaaat gatcgttacg
      541 agagagccga cgagaatact gcgacccact ccgccaatca caaagcccca aatgatcaca
      601 tgcgtaggaa caggcgctac caagatttct tcgatgtttt tctggaattt ttgagagaag
      661 aaggacgaag aaacgttggc atacgaacta gtgatagcag acatcataat caaaccaggc
      721 acgatgaatt ccatatatga aaaaccaccc atgtcaccga tccgaccacc gatcattttt
      781 ccgaaaataa caaagtacaa ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag
      841 atacgcaaat agcggttcgt ttccttgata gctaaacttt ttaatgcagt aaaataaaga
      901 ttaaacattt ttctcctccg tatgattatt ttcttctgtg attttcaaaa acagttcttc
      961 taaacgattc gacttattcc tcatagaaag aactttgatt ccttgtcttg tcagctgatc
     1021 gaagattccg ttcacccctt gatttcgttc aacttcgaca gcgattgtct gttcatcttc
     1081 aaatgtacaa gcgtatcctt caatcacagg tttggtctca tatggcgcta aatcaaagat
     1141 aaatgtttca aattgcagtt tggatagaag ctctttcata ctagtattct cgatcagttc
     1201 acctgattgg atgatcccga tgttgcgaca aagcatttca gcttcttcta aataatgcgt
     1261 cgtcaaaatg atcgttgtac cattttcgtt caattctcgg agaaactccc acatctcgcg
     1321 tctcaattca atatcgactc ctgctgttgg ttcatccaag atcagcaaac gtggttcatg
     1381 catcaatgcc ctagcaatca tcagtcttcg cttcattccc ccggaaagca tacgtgcacg
     1441 gacatttctc ttttcccaaa gattggactg cttcaaatat ttttcgctgc gtttcatcgc
     1501 ttctttgcga gagacaccgt agtagcctgc ttgattcacg acgatttgct ggaccgtttc
     1561 aaagggattg aaattgaact cttgcgggac taatccaatc tgttgctttg ctcggactag
     1621 atccgtatcc agatcataac cgaagacatt cacttttcca gatgttttat tgactaaaga
     1681 agtaatgatt ccgatcgttg tagacttccc agctccattt ggtcctaata gcgcatagaa
     1741 atctccttct ttcacggaaa gatcgattcc tcgcaatgct tcgaccccac ttccataaac
     1801 ctttttcaaa ttatttattt caagcgcatt tgccatttat ttcctatctc cttatagaca
     1861 tgattcttta ttgtatcatg gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa
     1921 atcaggttgc tgggctcagg aaaatctcct aatgaaagac atcaatcgga cgaagtcaga
     1981 caaccttttc agaaaaaaca aatcgaagat aaaggattac tattcatatg tgcaacaaaa
     2041 ggagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
     2101 ggaagcaaaa gccaagattt ttgatccaat aaatactttc gatcacttcg cctatcccaa
     2161 tacatgccac tgccgctttt catcaaataa cgcggcttcc gtttttgcgg tttctactaa
     2221 agtctctggg taatccaaaa ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc
     2281 ttcttttaat ttgaaatcaa aactgattcc ttttccttct gttacttgtt cttcaaagtg
     2341 gacattctca caataattct ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa
     2401 tgctaaagat ggatactccg caagccaccg aacgactgaa gcggaagaag caatccgttc
     2461 gatcgtattt gtccctttta aaatctcatc tacgaagcag tagcagcgtt ctttggaagc
     2521 cacttggtcc aacaaacgtt tgatcgattt gatttctgaa acaaaataac tgtcgccttc
     2581 aaataaatca tcttcgattg ccatggaagt caacacatgt ccaggttgca gggtaaactg
     2641 ttctgcaatc gctgtttgga tcgtctgtgc tagaatacag ctgatggcta tacttttgac
     2701 atacgtagat tttcctgatg cattggaacc cgtgactaat gtattcttct gccagttgac
     2761 cggattcact accgcttctt ttaacaatgg atgatagata tctttcgctg tcacaccgcc
     2821 ctcagtaaac tctggtcggc aagtgatggg catataagta cggtaattca atattgcagc
     2881 ggctacttct aattctccca gcaattccca cagcttgatg gcttcagttg aatgcttttc
     2941 taatttagtg agaacgaaat gataggagat aaatggcagc atgaacatga tgttgacata
     3001 ttcaaaaatc aactctgctt cactcccact cttcatacga aatgaaaaac ctaatttagg
     3061 gatgtctctt aatggtttag tttgctgttt cagctcttct tgaagtggtg tcggcagttt
     3121 tgctagctga ttggcggctg aaatcgttga aaccaaataa ctcatgctat tcagctctgt
     3181 ttctagtgtt tgtttcttca ctagataata gaccgtattg aatactaacc caccgattgc
     3241 caaatagatt cctagtactt gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa
     3301 gccaagaaaa ataaataaac ctacgcgtcc aatcttttta ctttttccat ccgctagata
     3361 ttgttttgca aaattatgat ctttttttcc taaacgagcg aattgatatt ggattttttc
     3421 tctcagttga ggattttcct gataaaaagc aatcagtttt tctaactgct gatcttcacc
     3481 aaaatcaaag ctgcgtaaac gttgatacaa cgcttctgaa cctacactag aataggtact
     3541 gttgattcct tcgaatactt cgaacatatc caggtcatac caagtaagat catcgatttc
     3601 gctatcccat gaacggaact tcttctccgt gagccatgct tccttcaagc tttcttcttt
     3661 atcaaatctc ggttgataag gaattttttc ccatttgcta cgaactgttt cctttaattt
     3721 caaacgtgaa aaaatgtcta aagcaactaa taaaataaca gctgcaataa taattaaaac
     3781 gataaattct ctttgaccca tatccctctc cgttccttta tttgttagct ttagtatagc
     3841 actgttgcgc tatttgaaaa gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt
     3901 tcttgtatat tacatattta tctctcattt atcaaaattg acaaataatc atagggagga
     3961 attgttgtta attcttgttt attctccagt gcattgacaa ctgtcccaag ggaaaggata
     4021 gcctcaggta ctattttttc ttgttctgtt ggattagcta agcgaagaac aaatccttgt
     4081 tcatgaaaat aagaaggata aatcgatgat acaattaagt cttcaggtaa ttcaatcaat
     4141 gaaaatttac gaggaacatc aagttcattt tgtaatggcc aaatcttgtt atctaacctg
     4201 tgaataaata cattcagttt ctgcttttga taagaaatag attgttctaa ccgtttttct
     4261 gcctgttttg ccactttgaa ctcattaaat cttccttctt ctacattaaa tccaaaagtg
     4321 actttatact cacctatctc ttgagctagc ggtgtttcca tcatgacatg cccttcattt
     4381 gtcgtatctc ctgaagctcg cccaggtcgc caagcaagat taggttttcc aagttcccct
     4441 gttgttgaaa ataaggttat gattaattga ttttctatac gttcatactc ttttaaacca
     4501 tctgcataaa acgtaaaata atgatcattt tctgccacag agacactctt atcgaatatt
     4561 tctaaattaa caggtttctc taccatagtt tctgaccaat tcggaggaat gacatcaggg
     4621 gtattttcaa taaatccgtt ttgaatttgt gcaattgatt tttcagattt atctaaaaca
     4681 ttaattgcta agcgcatacg atgcgagtaa acagtattat gaattttgag tgtgccttca
     4741 acgatttgac tttctcccag actgaacgat atttcataag atatttcttc catttcgggt
     4801 ttttcagata aacgatcttt taaatcttta ggaagttgag cttttccgta aacaactaat
     4861 gtttcttgta aagaatcttt atatacttta gctgtttcta attttagaga tagttctgta
     4921 tctccttcta atggtgaata atcatacgta tcgccatcat ttgctgaatc gatcaaatga
     4981 acgaaatcaa gatattgtct atctccaact atcaaattca attgcccatc tttaaaagaa
     5041 agtttatatt tattgttact tatctcagtt ttgtctatct cgattgctgt ttctaatttt
     5101 tcattatttt tctcaaattc aacaacagtg tatcctagtg ctggcacatt tacatttatt
     5161 tttatgtcaa gttcataata agctggttca tcaaaatatt cgaaacctgt agcaatcatc
     5221 cgataagcat ttttccgttc tggaaaataa acttcttttt caatgatatt atagggacta
     5281 cctttgaagt gtattttctt tgaatcagct actacatgca cgattttcga tccactataa
     5341 ttctcagtcg ttgtgttgaa aacaaggact tcattcgatt ttagctttaa tgcatcagcc
     5401 atccgtttta caataaggtt ttcgattccg tcagctatct cattagcttc tttgattcga
     5461 tggaaaatat cttccgcaac attatctgaa acacagccac caatactatc atgagcctgg
     5521 ttttgcaaaa tctttttcca taaccgacga agtaggcctt tactgatatg gataccattt
     5581 ctttctgcaa tcagcatgag cggctcaact cgttttagaa ttttttgttc caatttaaaa
     5641 ttttctattt tcatttttgt tcttactgag ccaatagaac gatgaacacg agagtaaacc
     5701 ggctgacgaa gttcaccttt atattcaggt aattcttttt tgtttctgat aacttctaca
     5761 taagaaggat aatctgaaat aatattgtgg aatttactgt atttattcaa cttctctacg
     5821 acttgatcaa aatttaaaat cattgacttt tgatctacac ccgaaggtac taaaatatct
     5881 ttgttttttc cttgcatatg aagcatttca actgcttcat ccaacttttc ttttgcaaat
     5941 tcaggaatat ctttaattgc gtctaaggtc atcattccag tcatataacc aaatggaaaa
     6001 ttcatggcat aaacaaattt atctcccaat cctttccaga taaaataagg agatggaacc
     6061 atcgtttcaa agtttaatcc tcgccaacac atgaaattat cgatttctac ttgatgcaaa
     6121 agtgtaggca aattcgcatt gaacccaaac gtgtcaggca aataccctac catcattgga
     6181 tctccatatt taaggatcgt atcactgatt ccaatcatta agtttcttaa aattgattca
     6241 gcatccacta ataaggcatc tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt
     6301 ttcatcagtc gttggataac cttaagcttt tgcggattga tctccaaata ctcatcgacg
     6361 attgaagttt gtccatctaa acaaaagttt actttagggt tcctttccaa ttcatctaga
     6421 acttcagtaa agacctgatc agacaatacg atggcatctt catttgtaaa ataccattct
     6481 ctgtcccagt gcgtgtgttg aattatatgt gcatttacca tatcagattc tcctactttc
     6541 cttcttctac tgaatttact tgttgttctt ttttatactt gatggatttt agtgcattga
     6601 ccattaacgc tgtaaccact cccccaataa caattgatac taaatataaa ggtatattcg
     6661 taacaaaagg aattgccata aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc
     6721 catttagacc acatgctact gcagtgccca ccacgatgct tggtattact cgtaaaggat
     6781 ctttcacggc aaacgggatc gtcccttcag taatccccac aaggcccata atcatagctg
     6841 tctttgcatt atctcgttcc gattcatcat aaagtttttt gtttaaaatt gttgctaaaa
     6901 acattccaaa tggcggaata gcaattccta ataacatggc tgtactaggt ccaaagcttc
     6961 cgcttgaaaa agaagcaacc ccaaaagcat atgcgatttt attgataggt cctcccatat
     7021 caaacgccat cattcctcct aaaatagccg acatcaaagc agcatttgct cctgacatgc
     7081 cttctagcca attagtcagt cctgttgtca ttgcttgaat aggcgtagct agtacatacc
     7141 ataatatgat tccaacaact aatgtagtaa caaaaggaat cagcataatt gattttagac
     7201 taataacttg catcggtact tttacttttt tcaatgcatt agctaaatag ccacaagcta
     7261 atccagcaac cattcctcca ataaatccca taccaagatt ttgacacatc atcccagtga
     7321 taaaagctgg tgctagaccg ggacggtcag caattgaata tgcgatatag gctgaaatca
     7381 tcggtacgat aaactgtaat cccgtctgac caatcattcg caggactccc cagaatgtat
     7441 ccgtctggtc ccaaacacct gtgccaccaa aaatgactgc aatagaagtt agtaaccctg
     7501 ctgctacgac aagcgggatc atataggaaa ccccactttg gaaatgattt agaatttctc
     7561 ttccttgttt cttgaattcc attagtaact cccccacgat atatttttta tttgttaatt
     7621 aatgcaattg cttcatcaat cgtctcttca ccatctttta ctacctctga ggtccctact
     7681 gttaaaatcg gtttaccgtc aaagcgacct cgttctgaaa catttgtatc tactgcaaat
     7741 atgacaacat ctgcctcgga aacatccgaa attgaaagtt tgttttctat ccccatagcc
     7801 ccctgtgttt ctactttaat atctaaccct ttcttcttcg caaacttttc taaattagct
     7861 tttgccatat aagtatgggc aatccctgcg atacatgctg ttactgctac tatttttgtc
     7921 atatgaaatt cctcctttta aaatgataga gcttttacca attctttttt atttctcgaa
     7981 tcttttattc ttttaacaaa ctcgtcatcc atcaatcttc cagataattc tgctaaaata
     8041 cgtaaatgat tagtaccttt atcagtgtct tttattgcta acaaaaagac gagatcaact
     8101 ggttgatcat ctaatgcgcc ccattcaaca gaccgcttta acttggctac agccactgtt
     8161 gattcaatta ctgctaggct tttgccatga ggaatggcga tatttttccc aattcctgtg
     8221 gtcatatggt tttctctgtc aaatacgtct ttttgaaatt gtttgtggtc agacacaaac
     8281 ccattagtct ctaacaattt cgataatttt tcgactacac tttttttatc agatgcattt
     8341 aaatctatca ctatattatt tatattgatt ttttcactga tcatttataa gccttctttc
     8401 aatatataaa ctaatttttc aaacgacgaa ctcgttttta aatttgctaa taactcatga
     8461 ttcgaagcga tttcattgaa tgtttcataa attttattca attcaggttt tactttttca
     8521 ttaagtgcaa aaaagaaaac agaatgaaca agtgtcccat cccaatcaat tccttctgga
     8581 ttaacgtaga cacagattac tggctttttt ataaaatcta cttcgatatg cggcatagca
     8641 aaatcattca tcgctgttga agatagtttt tctcttttga ttgctccctc tagtatccct
     8701 tctttcgcgt aattttcttt taccaatttt ttggtaatta tatttaaaac gttcacttga
     8761 ttatccttta tttggtgggt aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca
     8821 tcttctgttt gttcatttaa ctgtagatac tgttttactt ttctgacatc tgcccctaac
     8881 atcaaaggac taacaacaat aacaggaaaa tttgtatttt caatcggaat cgtgcttata
     8941 acaagttcat cagtgacttt tctatcttgt agttcattga tgcttagtac atcttctatc
     9001 tcaaccatat ctgcaaaacc gttatttatt ctttgctcta ataattttga tgttccatat
     9061 ccgctactac aaactaatat cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat
     9121 tgaatatgga gtgttacgta agcgatttca tcttcattaa aatgtataca gaatctctct
     9181 tcgatttcct ctaataaatc tacacttatc ataaatgacc ttttaaaaga atatttgatt
     9241 ttgtccgtat aagggttata gatatttact cccaatttga gtcttttaac tgctgcattg
     9301 agatgtaata ctagattatt gatcaattct tcatcaggat aaaattcatt taatttaatt
     9361 cttactaact cttgtagctc aacacccata tctaaatagg aaagcttcgt ttgatctaag
     9421 tcgtgattca tagcattttt ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca
     9481 tctattggca caataattga aaatgctttt tcaatcattt tgattaaaat aagtgtattc
     9541 tttgagattt cttcttcaga attattacta cgtgataaga aattgcttgt ttcaatcctt
     9601 tctatcgcaa tagcaaggtg gatagctaaa gtttgaaatt cataatctgt aaatttcaat
     9661 tgtgtcttat tcatgtatcg attaagcaat tcggctactt ttttcaatat gtccttattt
     9721 aagaaattat tcgcttcacc aatgaaattg atattcaagt ctaagagatc tgttttactg
     9781 ttagatgctg taaagccacc ccaataatag ttcacaactt ctgacatcaa ccttctttta
     9841 cttttttcat cagctttgat gcgcacgcca tttcgatctg aaactaagga catcccatgc
     9901 ttgtttagtc tttttctaac atctttcatt gtgctttcta ccgtcgacct gctcacgtat
     9961 aattcgtcag caagattttg aatctttaga gaatcattag tattcaataa tttagcaaac
    10021 acataagtta gacggtcctc cggagagtct gcgatattga ttttatgact ggataataaa
    10081 tttcgaagtg tttctttttt tccaataagc gaaacgcctt ttttaggttg aaccaacatt
    10141 tgtacatctg gcattttttg gattatttct ttcaaaatta aaatatcttt gctaattgtt
    10201 ttcgtagata catataattg ttctgataat tctttgtaag ttgtgtgctc attttcaatc
    10261 aaaaagtata gtattttcat ttgccgttcg ctaaattcga tgcccattat cttcacctcc
    10321 tgaatacagc ttaagtgtac aattattatc tctattttca tacagaagaa agttcttgat
    10381 taatgtagaa gtttctaata ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt
    10441 tatatcacag gcttttttct ctattaaata tatataagat aatacatttt tctactagtt
    10501 ctcactaatc ttgtataatt aaaccctccg caacttgacg gagatggaga agatgccccc
    10561 gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg acaggtaaag
    10621 cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa aacgctggaa
    10681 gactttttca acagaggttt gcgttcctgg tttgatagga ttggccactg ttaatcgaat
    10741 gaatggatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac gcaataaatt
    10801 ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata cagttgtatt
    10861 tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt cataaaaaga
    10921 gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca tttctttttg
    10981 cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta atcttgcaac
    11041 agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta cctgttccag
    11101 taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag aaaggccgac
    11161 gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt gttcattttc
    11221 atccaaccat gtattcagtg catctactag gtccattaga ttcttttctc gaaattcgac
    11281 tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct tagttaatct
    11341 cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa cgagaagcaa
    11401 cagccattta atcaaatcta aaccccactc cccacaccgt atgaatatac acagcatgtg
    11461 cctttgctag cttccctcga agattggaaa tatgcatatt cactgttttc tcactatcaa
    11521 aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact tttttcggat
    11581 gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca gccacaccat
    11641 tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag atgcgttgcg
    11701 acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa
    11761 gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa
    11821 ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc gatgtctgtc
    11881 ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct agaaggatca
    11941 aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa gcatcaagaa
    12001 gtttgtatct ctgttcaaga acctcctcca gtaaggaatg aacagcttca tcgtcttcga
    12061 taatcaaaat agtttccagc aggaccacct acttaatgtc agatttttgg aaaataaaca
    12121 ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt ttcggtgctt
    12181 gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc gtaaaccact
    12241 ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaatgaag gccgttccca
    12301 tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg aatgtcaaag
    12361 ccactggaac agataatgtc cctaatgtca gcagaagcaa tacaggaaat acttttagaa
    12421 cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca agtgtgtaag
    12481 caagcatggc tagtcccgtg atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga
    12541 gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta ttgcgaatcg
    12601 taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa
    12661 tccatgcagc cattttttct accattaaaa tccctgttgc accgttgatg actgtatccg
    12721 ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc caagaataaa
    12781 aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc ttccatactt
    12841 ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa ttgtaagaaa
    12901 taatcttcca gtttttgccc aactaggtga ataccatcca caggaacttt tgcttctacc
    12961 aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat aacacggcta
    13021 gattgtacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc
    13081 gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc tttcgataat
    13141 tctttgatca agtgtccttc atgcaaaata ccatagtgag tggcgatctg tgacaattca
    13201 tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa acgttggatg
    13261 atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc atccaaaatc
    13321 aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg catacctaaa
    13381 gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt cagcaggtct
    13441 tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt atctcttgca
    13501 gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg tcttgctgcc
    13561 tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat caaacccata
    13621 atagttctca taaaggtaga cttacccgct ccattttccc caatcaatcc atagatcatt
    13681 ccacgtttga tttcgattga tactttatct aatgctttgg cagcaccgta ttttttggtt
    13741 ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct tctttcttat
    13801 tgatgagtga agtatagttg acgggtataa agaaagcagc cagaaatgta taaagaaagt
    13861 gtaaattttc caattgaatg tcaaaaaaaa caatcggaca atacgtgtct ttcctattat
    13921 caggcagttt gttatacgct tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt
    13981 ttctctgtat tcctcttcac tgaaaatttt tgcttctttc agatcaagct tgagtaattt
    14041 taccagaccg gttataagga taactatagc aagactccct cccagaaaaa ttagactgtc
    14101 tcgcagacgt ggttgcgttg tcttccaatc aaagagcgca ccaaacagtg ctaatccaag
    14161 tggactggcc accatggtaa tcgttgttat cgttgacata agtcgtcctt gtatatgcgt
    14221 tggaacttct cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa
    14281 acggacttat gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag
    14341 gttatgacaa aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac
    14401 tcctcggtca caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc
    14461 ttatttcttg aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt
    14521 gatgatactt tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat
    14581 cctacctatt aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc
    14641 agaaaagtta ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta
    14701 actcgtgaac tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc
    14761 tttcttaaga cctggttttt tcttcgtatc attgaataag tttgaaaaac cttgatttat
    14821 aaaggttttc ccgtaaatat attttattat atttgattaa atttgaaatg tttgtgttct
    14881 aaaatgtgtt ctaatttaaa ggcagtatat ttcgtatttt agattaaatt tttaggctca
    14941 ttcattctca aatccattct attcagtcaa taaaaatttt aaaaagactt tataaacaaa
    15001 gatgttcaag cgagcatctt atttttttgt gcttaatttg aaaggagaac atttcatgta
    15061 taaaaaacaa aaaaagaaat tatgtgtatt aactattttg agtttggctg ttcaagcaat
    15121 tgttggagca attgctccta cgattacttt tgctgatgaa attacccatc cacaaacggt
    15181 aaccgtagaa ctagatttag cacatcaata tgctgttgaa ggaactttta gtgatggtcg
    15241 tccaatgtca gaagtcactg ttccgcatta tgcagtgtac aacggcttta aacaagatat
    15301 tttctgtatt gaaccaggag tcccgattta caatgagttc actcctggct atgagaaaaa
    15361 cccgttacca gatatgtccg ataaagccaa attagtttcc gttttatgga aaaaagtagg
    15421 tacagatgca gatactcata ttgtggcaca gaagatgatc tggcaagaag ttaatgggta
    15481 tacccttcac tcaataaaac gatcagatgg tagtgccgta aatatcgcag ccattgaggc
    15541 aaaaataaat caagccattg acgattatca gaagaaacca accttccata atagtacggc
    15601 taaaacagta ttagggcaat cgactactgt gacggatacg aataatctga atttgtcaga
    15661 gttcgatgag gttgtggaaa atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt
    15721 gataatcacc ccaaatgcca attcaaaaga aagtggtgtg ctgacgctta agaaatctgc
    15781 tggtaccgga acacctgtcg cctataaaaa ggctggccaa cagactttaa tggccggggc
    15841 gattgataaa cctaatacct acacaatcaa aattgatgtg gaaacggagg gttctttaaa
    15901 gatcaaaaaa gtcgataaag aatcgggtga tgttgtacca ggaacggttt tccatttaga
    15961 ctttggaaaa actttacctg caaaagacgt gactactgac aaagaaggca ttgctacatt
    16021 ggatgagatt cctcatggta caaaagtaac cattaccgaa aaatcagtac cggcgcctta
    16081 tacgattgat accaccccca tgactaccac cattaaagca ggcgaaacca tttacgtgac
    16141 ttcaaaaaac gcacgggaaa aagggcaaat cattctggat aaaagcggag ttgaaacagg
    16201 gagtgatctt tggaatgaca attattcctt agctggtaat acgtttgcca ttcgcaaaga
    16261 cagtccaacc ggtgaaatcg ttcaagaaat gaccacggat gaaaacggtc atgcggaaac
    16321 accaaaagag attgctaatg cgttggaatt gggaacctat tatgtgaccg aaactaaagc
    16381 tagtcatggg ttcgtgaata cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca
    16441 aaccgtagcc cctgttacca gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac
    16501 cactttgacg aaagaagaca aagatactgg ggataaagca caaggtaagg cagtctttga
    16561 gggtgctgaa tacaccttgt ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc
    16621 ttttaaacca gagttagcga agggaacgaa aggttctgat gaaacggtca ctttgacttt
    16681 agacgaaaag aaccaagttg ccgttaaaca tttagctatc aacgagtatt attggcaaga
    16741 aacaaaagcg cccgaaggat atagccttga tgaaacaaag tatcctgtat ccatcaaaaa
    16801 ggtggatgat agcgaaaaaa atgccgtgat tacccgaaat gttacggcaa aagaacaagt
    16861 gattcgcttt ggctttgatt tctttaaatt tgctggatca gctgcgggca ctaccgaaac
    16921 tggcttcaat gatctgacct ttaaagtgtc gccattggaa gggaccaatg aaatcacagg
    16981 tgccgaagat gaagcgacca cagcttataa cgagcaatta gggtttgatg gttatggcaa
    17041 gtttgaaaat cttccttatg gggattattt acttgaagaa gtagaagccc cagaaggttt
    17101 tcaaaagatt acgccattag aaatccgttc tacttttaag gaaaacaaag aagactttgt
    17161 gaagagtgag tatgtcttta ccattaccga acaagaccaa aaacaaccaa ttaaaacggt
    17221 gaccgttcct tacgagaagc tgacgaacaa agcattttct gttagtttga accgtttaat
    17281 gctctatgat ttgcctgagg aagaagatag tttaacttct cttgcgactt ggaaagacgg
    17341 aaacaaagaa ttaacttctc tggattctac cgagctagtt gataaattga gttacaatct
    17401 acatgaaatc aaagaagact ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa
    17461 agctgcccaa gaaaaagatg aaaaagccaa accagtggtg attgcagaaa caagcgcaac
    17521 gttggcgaac aaagagaaaa caggaacttg gaaaattcag cataaattaa ccgccgaaca
    17581 agttttaaat aaaactattg tcttattcaa ctatgtgtat gaaaataagg aagcttttga
    17641 agcaggcgat aagccagtag cgaaggatgt tagcttgaat aaccaagccc aaactgtcag
    17701 ttgtacagtg gaacaccatg tttctatcca aacaaaggcc cacctagaaa acggttctca
    17761 gacctttact catggtgatg tggtggatat gtttgatgat gtgtcgatta ctcatgatgt
    17821 actggatggc tcaaaagaag ctttcgaaac aattctgtat gctttattac cggatggtac
    17881 aaacaaagaa atttggaaat ctggcaagat tgattatgaa gtaaatgata aagaattcac
    17941 caaaactgtg cttgcgaaaa aagtagatac cggaaaatat ccagaaggaa ccaagtttac
    18001 ttttgcggaa atcaattacg ataaagatgg gaatatcaat ggaaaacaca atgaagattt
    18061 gaaagaaaaa tctcaaacct taacaccaaa agaagttcca accatactaa gtacgccgaa
    18121 acaaccggaa acaccaactg ttccaagtgg ctctcaagaa tctagtccca cagtgaagac
    18181 cttccctcaa accggggaga aaaattccaa tgttttactg ttcattggtt ttactttgat
    18241 ttttgcgacg gcgggctatt atttctggaa tcgccggaac taaggtgatg agatgataca
    18301 agtaaatcct aagaaccaac gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt
    18361 gtatccaaaa ctaacaatga aaagaggaaa agaagatgga attgaaattt gtcgtgccag
    18421 atatggctga aacctttgga aagttaagtt atgccggtga aggagaaatt ataacagaag
    18481 gatatggacg gaatactacg gtgattggtc gcagttatca tttgtattcc agtaagcaac
    18541 gggccgatga tattgaggtg gtagtagctg cggaagccgg tgaaaaggat tttgatcaag
    18601 accaaccgct aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18661 gtgggtttac tgattacgtg ttgtatgttg atgatttagt gaaagcatag gaggaaaaga
    18721 agatgagatt aacagaaggt attgtcgtag attcaggttt aacatttggg aagttgcgtt
    18781 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacaatt ggtaatgagg
    18841 taaaagaacg aacctataat ttaaaatcct ctgcacaagg gcgaatgatt caagttagta
    18901 tcccagctaa tgtaccctta cgcgagtttg cttatgatgc agaggtagag ttggtcaatc
    18961 caatcgtgga taccgttgcc aattatgttt ttcgagaagg aactaccgtt aattggttca
    19021 ttaaggcgga tgatttagtc ttgaagcggc agccgaatca aggaaatcct acgaaccaaa
    19081 atgaaagaaa gaaataggtg aagattatgg aaattaatgt tgaacatatt ttagattgtc
    19141 tcgatcaata tggaaaaggc gagctgactg aggaacaact cgttaaagca ttaacctctg
    19201 atgaaaaaat gtttctgatt atacatcaag gtttacttgg agaaaatagc gacagtgaag
    19261 aagactctga tgtactagac tggttaggag aagaagagtc tttctttata gttgtcgaag
    19321 taaatgaaaa cttatgtcgg caggcagaat ccattttgga agaaattggt gtggagatgc
    19381 cggatgctat tgaaggcttt ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt
    19441 agcagtaaat gactagcata actttttgaa attgcctaat gaaatcctat tgggcttttt
    19501 cgattggagg tggaaaccat caaaatgtac aaaggacatc gcatacgcgc aggagatcag
    19561 cacttggttt atcactttgt tcttggctgg ctactcgctc tgttcatcgg ttggatgagc
    19621 gtcttttatt ttcaagagtt tagacaattt gatatttcta agctatcact ttctactatt
    19681 gaaatcgttt ggtctataaa agatttagta tgtctgcttg gaagtctcgc tttttcagga
    19741 gctatgatat tgctttatat tcactttttt cttgatcatt ggcgaagctt gtggcatcga
    19801 caaaagctag ctcggatgat tctagagaac cattggtatg aagtgaaaca aactcaaagt
    19861 gaaggctttt tcaaagattt gaatagtagc cgaaccagag agactatcag ttacttcccc
    19921 aaaatctgtt atcgaatgaa agatggatta ctgtctattc gcgttcaaat ttcacttggg
    19981 aaataccaag atcagctttt aaaattggaa aagaagttag aaagcggact gtattgtgag
    20041 ttagtagaaa aggaactcaa agactcctat gtggagtata ctttactgta tgatatgatc
    20101 gccaatcgaa ttgggataga cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa
    20161 aatcaagtat gggcttatga ttctttaccc catatgttaa tcgctggtgg cacgggtgga
    20221 gggaagacct atttcctcct caccattatc gaagcactat tgaagtcaga tgcagaattg
    20281 tttatcctcg atcccaaaaa tgcagattta gctgatttag gtacggtgat gcctcatgtt
    20341 tattcacaaa aggaagaaat ttctgcttgt gtggaagatt tctatgaacg tatgatggct
    20401 cgtagcaagg caatgaaaga aatgtctaac tacaaaacag gagagaatta tgcctatctt
    20461 ggacttccac ccaacttttt aatctttgat gaatacgtgg cttatatgga gatgttaacg
    20521 acgaaagaaa gtgcggtgat tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa
    20581 tctggtttct ttctgatttt ggcttgccaa aggccggatg cgaaatattt aggagacgga
    20641 atccgcgatc aattcaactt tcgcgtggct ttgggtcgta tgagtgaact cggatattcc
    20701 atgatgtttg gggaggtcga aaaaaacttc tttatgaagc acatcaaagg tcgaggttat
    20761 gtggatacag gtggcagtgt gattagtgag ttttatacgc cacttgtacc aaaagggtat
    20821 gatttcttag attcaattag taaattagaa aagatgaatg attgagtgat cttcaatact
    20881 gactttatac ttcaagtaac gtataatact tttatatagt aggaagaaag gggataatta
    20941 atatggaaag taatgattct tatttagagt atgcaaaagc aaataaggat aagtttattt
    21001 cttatattat caaagggaaa tcccccgaag ttggacgaga cgcagttttt atggcaggga
    21061 gtcctggggc tggaaaatca gaggttgcca ttggattagc tgagaattat gataaccatg
    21121 tgattattga tgcggattac tttcgcaccc agtttcctga ttataatgga aaaaactcta
    21181 gtctctttca aaaagcatct tcttggttgg tagagcaggc atttagatat gtcttagatc
    21241 atggctattc ttttattctg gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct
    21301 cgcgggcgtt gaaaaaacat tttcgagtga cgattttcta tgtttatcaa gaccctaaaa
    21361 tcgcttggaa atttacacaa gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg
    21421 catttattaa cgcttttttc aaatcaagag agaacattgc aaaagtaaag agtaaacatc
    21481 cagatgtatt gcttcatatc ataataaaag attatacgaa taatatttcc gaagttcatt
    21541 atgctacgga taatctagaa ttatttttac cactagggta tagtaggaaa gatttggagg
    21601 ttcaattaca tgactgagaa agaacaagta acgaaaattg taaagaaata caataagagc
    21661 attgcggatt tatcggaaaa tgctactgca aaagagttca aaacggtgat taaatatgta
    21721 gccgatcagg ccaatgaaaa acaacggaaa ttagttggat taaataaaaa atagtaggac
    21781 aaattaataa tgtatgagcc aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg
    21841 aggtaatgta tgagattttt agatttattc gccggtattg gtggctttcg tttaggtatg
    21901 gaacaggcga gccatcattg tatcggtttt tgtgaaattg ataaatttgc tagacgtagt
    21961 tataaagcaa ttcatgatac aagtaaggag gtggaaatgc atgacatcac aagtgtatca
    22021 gatgagttta ttcaatccct cggaccagtg gacatccttt gcggcggatt tccgtgccaa
    22081 gctttttcaa ttgcgggaaa gcggcaaaga ttttccgata ctcgaggtac tttattcttt
    22141 gaaatcgctc ggttcaccac tcttctccga cctaagcttt tattccttga gaacgtccga
    22201 ggattgctca atcacgaagg aggggctacg ttcgagacga tcctccgaac gctggatgga
    22261 ttggggtatg acgtggagtg gtaggtgctt aactcaaagg cctacattcc ccaaagccgg
    22321 gagcggatat tccttatcgg acattctcga gacgcatgta ccgaacaagt atttcctatc
    22381 attggaccgt cttcaacatc tgatcaaaac atcagaaatt tggtaaatat caatccttcc
    22441 aatcgaggaa tgggtggaca agtctatggt tcagatggcg tagcgccaac cttaacaggt
    22501 gatgaaggaa tcaaaatcgc actgccagca agggatggaa tctctgtagc aggactatta
    22561 ccgggaaagt ttgaacaagg taatcgagtt tatgaagtta ctggaaccgc ccccaccttg
    22621 tcaacgaaac aaggtggtac aaaaatcatg attcgaaaaa atgaaacaga atatcaagag
    22681 gtgaagccag aagacagtgt gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt
    22741 ctgggaaaac agagtgttca taccttacta acaggagacc aacaagcgat cgtcacggat
    22801 cagtatcaaa ttcggaagct aacacctaga gagtgttggc gacttcaggg ctttcccgat
    22861 tgggcttttg atcgagattc tcaagtcaat tcagatagtc agttgtataa gcaagccgga
    22921 aactctgtaa cagttccggt catttttgat attgccagaa gattaaaaga gttggatgta
    22981 catggtttat gatgagttga ttggagaaat ttattgggtg attggaaaga ttcagtcaga
    23041 cccagaatta gaagaacaat tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa
    23101 agtcgctggc gatccatatt taatgaatga ggagacggat gctcgaattg aaataaatca
    23161 agtaattgca gaatttgagc ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata
    23221 cttgttagaa atgaaagcag agttggaagt aaatggtatt acagatgagt aacctaaacc
    23281 aacacaactg actgttaggc gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg
    23341 tgctgtgcct gttcatggac ggcgttagcc gaccatgatg tctcaccccc gtatctaaca
    23401 ggggggtaca aaaacacaaa aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa
    23461 gatagtaagt aatttgtata ggtgttaact catttgaaaa agttaacacc ttgaatttgg
    23521 aggtgagaac ttggcacaac ggaatttaga ttatcggcta ttaaaagacc gacgcaacga
    23581 atacggcgtt tcacaaaata aattggctac ggcttgtgga cttagtcggc cgtacttaaa
    23641 tcagatagaa aatggtggcg taaccgcatc tataaaaacc atgaggaaaa tttttgacca
    23701 actggaaagc tttaatcccg atttgccttt gacgttactg tttgactatg taaggattcg
    23761 ctttcccaca acggatgcac gaaaaattat tcaagaaatt ctccatttga aatttgagta
    23821 tatgctccat gaagattatg ccttttactc ctatcaagaa cagtatgtca tgggtgatat
    23881 tgtggtgatg ttgtctcatg aagaagataa aggtgtgctt ttagaattaa aggggcgagg
    23941 ttgccgacaa tttgaaactt ttttactcgc tcaaaagcgt agctggtacg actttttcga
    24001 ggattgtcta aaggccggtg gcgtgatgaa acgcttggac ttggcaatca atgatcgagt
    24061 ggggctattg gatatacctg agctaacgaa aaaatgtcag aaggaagaat gtatctcctt
    24121 gtttcgtacc tttaaaagtt atcgttccgg ggaactttta aaggctgatg aaaaggacgg
    24181 catgggcaat accttatata tcggcagtct caagagtgag gtttatttct gtttgtatga
    24241 aaaagactat gaacaatata tcaagttagg gattccatta gaccaaactg agacgaaaaa
    24301 tcgttttgag attcgactga aaaatgaccg tgcttttcat gcgattcaag atttgttaaa
    24361 aggtcgtagc attgagagca ccacgttttc cattatcaat cgttacttgc gatttgcgga
    24421 taaagtagaa ggtaaaaggc gaaccaactg gcccttgaat gaacaatggg ggcgttttat
    24481 tggacggaat cggaaagaga ttcaattaac ttccgagcca aaaccgtata ccattgaacg
    24541 aaccttgaat tggctaggcc gacaagtcgc ccctacgtgg aaaatggcta aggagttgga
    24601 tcgattgaat cggacaacct atatccagga tatggtacag aatgcacgct tatcagaccg
    24661 acataaaaag attttggagc aacaaagtat ggcaattgaa aatttgatta tatgaaaaga
    24721 ggaatcgtca tgggaattgt aaaagatatt tttttggtat ttggtggcag tatgcttggt
    24781 gttactgtga tgtgcttgat gaatgcaagt gcagcagcgg atcgagcaat ggaaaagaaa
    24841 ggaaagtgac aagatgaatt tcggacaaaa ttt
//
LOCUS       DACDXE010000040        24036 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.040, whole genome shotgun sequence.
ACCESSION   DACDXE010000040 DACDXE010000000
VERSION     DACDXE010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.040"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(27..725)
                     /locus_tag="IWM23_001629"
     CDS             complement(27..725)
                     /locus_tag="IWM23_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAP9884266.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(718..2070)
                     /locus_tag="IWM23_001630"
     CDS             complement(718..2070)
                     /locus_tag="IWM23_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAP9884267.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2293..3693)
                     /locus_tag="IWM23_001631"
     CDS             complement(2293..3693)
                     /locus_tag="IWM23_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAP9884268.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3854..4591
                     /locus_tag="IWM23_001632"
     CDS             3854..4591
                     /locus_tag="IWM23_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAP9884269.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4716..5195
                     /locus_tag="IWM23_001633"
     CDS             4716..5195
                     /locus_tag="IWM23_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAP9884270.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5192..5959
                     /locus_tag="IWM23_001634"
     CDS             5192..5959
                     /locus_tag="IWM23_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884271.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6189..6758
                     /gene="lepB"
                     /locus_tag="IWM23_001635"
     CDS             6189..6758
                     /gene="lepB"
                     /locus_tag="IWM23_001635"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9884272.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6862..7464)
                     /locus_tag="IWM23_001636"
     CDS             complement(6862..7464)
                     /locus_tag="IWM23_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9884273.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7570..8039
                     /locus_tag="IWM23_001637"
                     /pseudo
     CDS             7570..8039
                     /locus_tag="IWM23_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8145..8729
                     /locus_tag="IWM23_001638"
     CDS             8145..8729
                     /locus_tag="IWM23_001638"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9884274.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8732..9805
                     /gene="prfA"
                     /locus_tag="IWM23_001639"
     CDS             8732..9805
                     /gene="prfA"
                     /locus_tag="IWM23_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAP9884275.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9798..10637
                     /gene="prmC"
                     /locus_tag="IWM23_001640"
     CDS             9798..10637
                     /gene="prmC"
                     /locus_tag="IWM23_001640"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAP9884276.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10630..10809
                     /locus_tag="IWM23_001641"
     CDS             10630..10809
                     /locus_tag="IWM23_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884277.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10831..11856
                     /locus_tag="IWM23_001642"
     CDS             10831..11856
                     /locus_tag="IWM23_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAP9884278.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12031..13275
                     /locus_tag="IWM23_001643"
     CDS             12031..13275
                     /locus_tag="IWM23_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAP9884279.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13428..14375
                     /locus_tag="IWM23_001644"
     CDS             13428..14375
                     /locus_tag="IWM23_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9884280.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14509..15138
                     /gene="upp"
                     /locus_tag="IWM23_001645"
     CDS             14509..15138
                     /gene="upp"
                     /locus_tag="IWM23_001645"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAP9884281.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15277..15519)
                     /locus_tag="IWM23_001646"
     CDS             complement(15277..15519)
                     /locus_tag="IWM23_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884282.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15640..15712
                     /locus_tag="IWM23_001647"
     tRNA            15640..15712
                     /locus_tag="IWM23_001647"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15673..15675,aa:Thr,seq:cgt)
     gene            complement(15831..16469)
                     /locus_tag="IWM23_001648"
     CDS             complement(15831..16469)
                     /locus_tag="IWM23_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9884283.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16592..17224)
                     /locus_tag="IWM23_001649"
     CDS             complement(16592..17224)
                     /locus_tag="IWM23_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9884284.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17575..17874
                     /locus_tag="IWM23_001650"
     CDS             17575..17874
                     /locus_tag="IWM23_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9884285.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            18369..20534
                     /gene="acm"
                     /locus_tag="IWM23_001651"
     CDS             18369..20534
                     /gene="acm"
                     /locus_tag="IWM23_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAP9884286.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(20806..21291)
                     /locus_tag="IWM23_001652"
     CDS             complement(20806..21291)
                     /locus_tag="IWM23_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAP9884287.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            21771..22412
                     /locus_tag="IWM23_001653"
     CDS             21771..22412
                     /locus_tag="IWM23_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9884288.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            22431..23066
                     /locus_tag="IWM23_001654"
     CDS             22431..23066
                     /locus_tag="IWM23_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9884289.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            23084..23899
                     /locus_tag="IWM23_001655"
     CDS             23084..23899
                     /locus_tag="IWM23_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9884290.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         8319 a         4090 c         4778 g         6849 t
ORIGIN      
        1 ttgtcacatt cccctttttc gaataactag gctgtttgtc cttcaagata gggcagatcc
       61 aattctttac cgtaacgttg ttctaaagct aggcggatca aacgagctgc caggttctca
      121 tttcttgcaa ggataggtcc atggaaataa gaaccgaaga catttcggta aatgacacct
      181 tcagattggt cttcaccatt atttccttga ccttgtacca ctttccccaa aggtcgttca
      241 ccttcaccta agaaagtccg accattgtgg ttttcaaatc cataataagt ttcattgaat
      301 tcttcgttat ggataacgat atcaccgata aaccggttat tttcctgact taacgtgtag
      361 tgatccagtg cactgattcc ttggatcttt tctccattag ctcccatgta gtagtgacct
      421 agcaattggt agccaccaca aatggctaac atgacgccat cattttcgat atagttgatc
      481 aaagaatctt tctttttctg gatgtctttt gaaatgatta attgctcaaa gtcttgacca
      541 ccgccaaaga aaaccaagtc atatttatca gcatcaaaag gttcatgtat actaacgatc
      601 tccgtatggc aagaaacccc cattttttct gctatatatt tcaacatcaa aaggtttccg
      661 ttatcaccat atgtgttcat caaatttcca taaaggtggg cgatattgag ttcataatta
      721 gccacggttc attcctcctt tgataaatcc ttgagcagct agttctttac gaagctgcaa
      781 tacagcagtg taagtagcta aaatatagac atgctcagtt ggcaattctt tgattttttc
      841 gattacttct ggtaattctt ttgtttctgt caatttattt tctggaatcc cggcaacttt
      901 tagacgtaaa gtcatatctg tatgacggtc tcctccagaa ataacagctg ggatatccat
      961 atcggcaaag ttttcaaaat taccatccca aatccagctg acatcgattc cgtctgcgta
     1021 attggcattt aataaagcca ctaaagaaaa tggataagga gctagaccaa tcatatcgat
     1081 tacttgattg atacctacag gatttttgac aagtactaat gtacattttt tcccattgat
     1141 attgatgact tcttgtcggc cgaatacttt ctcgtcatat cctaaacctt gtttgatttt
     1201 atcaggatcg acattgaagt actctgccac tgcagtagct gctaaagcat tgtagacatt
     1261 atacataccg ccaacttcga ttccgtattc atgaccatcg atcacgaaat ctgctgacac
     1321 attgtccatc cgtaccatat cagttaatcg ataatccaat tcaggacgtt tgaagtcaca
     1381 atttggacag taatatttcc ctaagtttgc ataagtgatc attttataat gcaagatgtg
     1441 atgacaattt ggacataaga caccgtctgt attgtaatga gccatttgtt cacgatcttc
     1501 ttcatggtca aagccataat attttctagg attgactgtt tcgatagaat tgaagattgg
     1561 tgaatcaccg ttactgataa tcgttgcatt tggtgacttt gcggcaccgt ctacaatcat
     1621 tttataggtc gtatagattt ctccataccg atccatttga tcacggaaaa tatttgtaaa
     1681 taagaacagt tctggtttca tatattctgt gactttgctc aaacttgctt catctatttc
     1741 taggacagca aactttttct gtcctttttt tggttttgcc tgcaagaaag tcgatacaat
     1801 tccctgtacc atattcgcac cagtagggtt ggttagaact tcatcgaatt gctggcgcaa
     1861 aatattgact gttaaagcgg tagtcaatgt tttaccgttt gttccggtga caacgacgac
     1921 ttggtagtct tttgccaaac tatctagtat atgtggatcg actttcagcg ctagtttacc
     1981 tggtaaactt gatccgcctt taaaaaaagt ttttaatgcc cattgcgacg tttttccaac
     2041 tgcaatcgca aaatgactgc gtattcccat tatgtaacct ccaaaaacga tttgctgacg
     2101 aataaattca actaatcata ccatatgttg aaaaaaaggt taaattgctt ttcttattct
     2161 taagaaaagg ttacggctga aagtgcatga aatcggacaa aatcgcatga tttaacaaaa
     2221 aaacagcttg aaacaaaatg attgtttcaa gctgctgtga ttttattatt ccctcttacc
     2281 tatattccca ttttaaccga taataatttt ctcgcttgga tattcatagc ctagatctcg
     2341 tgtttctttt ggcacgaata acatcaatgt atatagcatt ccgatccgcc caataaacat
     2401 cagtgtagca atcgtgattt tgcccacaac agacaagtct ccggtaatcc ctaaagataa
     2461 accagttgtt ccaaacgcag aagttacttc gatgatgatc gagatcagcg tttgttcttc
     2521 tgtagcagat aaaaaaacga tacaaaagaa acacatacca agagaaagca taaagacaac
     2581 gactgatttt cgcacatcat cttgatcgat tctccgtcca aaaatattga tgttatcttc
     2641 acttttgagg aaggaatata gatacaaacc gatgattgct acagtagttg ttcgaatccc
     2701 gccgcctaca gaactaggac tgcatccgat aaacatcagt aaagaaaaaa tgatcagtgt
     2761 tgtgatttga aaatcaccca aatcgtgaat ctgcagacca gcattacgag tcgtaatcga
     2821 ataaaacatc gaattgatcc atttgacact catattggaa tcttggaata aatggtcctt
     2881 ttccaataga taaatcaaga cagttcccga aacaaacaag ataacaaaag ctaacacagc
     2941 taatttggta aacaaggaaa aacgaaacgg aagcttcgca ttggaacgtt tgtataatag
     3001 ccattcccgg cactccatca atacaggaaa accaattcct ccgacaaaaa tcaaaaacat
     3061 gatcaggatc aagaaaaaat agtcatgtgc aaagggcttg atggaatccc cagtgacgtc
     3121 aaacccagag ttcgtgacag ccgatatcgc ctgataaaaa ccgtaaaaaa cggcatcacg
     3181 ccaacgatca aaatagcctc tgtaataaaa ataaatagaa aagaatgtcc caaaaagtaa
     3241 ttgaaaccag attaaaatag caaaagtgat ccgaatcagt cggacgatac cagacaaacg
     3301 cggttgattc atatccgtca tgatcagctg tcgctgtttc agtgtgatcc gtcgtttaga
     3361 aaaaatgaca aaagcagtag aaatcatcat gatccccaaa ccaccaactt gaaataatat
     3421 ttccagcagt ataataccgt tatcattgaa cacagaattg atatcaaaag tcgatagtcc
     3481 agtcacactc actgtactga ttgccatgaa cagcatgtcc acaaaagaaa catggctatc
     3541 tggttcgcga aaaatcggca tataaaatag aacgagtgat aagaccgtca tcaatatata
     3601 ataaaaaacg atgatttgga tcgatgagaa atgattggca gcaaacttct gtcctcgccg
     3661 ctgcagcctt cgaaagaata aatcaacatg cacagtacta tcctcctgac aatagtcgtt
     3721 tctaccagta tacaagggga attaggaata ctcaaagaaa ttatcaaaca gataacaata
     3781 cagctttcga ttaaaataga aatggcttat aataaggaaa atcctagaaa aaagaaggag
     3841 gaggaataac aaattgatta atgctcaaca aatcgtagaa aaaatgaaaa atcaaaaaat
     3901 aaattatgat acagttctga aaaaaatgat catgcaatgg gagaaagaag aagtaaagcc
     3961 taagatatta atacatacat gctgtgctcc ttgcagtacg cacacattgg aattcatgtc
     4021 tcaatatgca gagatcacgt tatttttttc aaattcgaat attcatccta aaagtgagta
     4081 tttgcgaaga ttacatgaac aaaaacgatt tgtagaccaa tttaatgaga aaactgacca
     4141 tcatgttgcg ctaatcgttg atgaataccg tccaaatgaa ttcaccaaaa tggttttaac
     4201 aaaccatctt gaaaacgaaa aagaaggtgg tgctcgctgc agtgcatgct tcaatatgcg
     4261 attagatctt gttgcgcaaa aagcgcaaga actaggatac gattactttg gaagtgccct
     4321 gaccatctcg ccaaagaaaa atgcagcttt gatcaatcag atcggaatgg atatccaaaa
     4381 gatttacggg acacaatact tgccaagtga tttcaagaaa aacaaaggct atgaacgctc
     4441 tttgcaaatg tgcaaagagt atgatattta tcgccaatgt tattgtggtt gcgtgttcgc
     4501 tgccaagcag caaggatgcg atctgaaaga aatcaatcgg gaagcaaaag cttacttaaa
     4561 aacatcaacc gtagtttttg aggaaaatta aagaaatctt tcggaaaaac gtaagaaaaa
     4621 aataaagaat ttatggtaga aataaggtag actgtataat ttgtccgtag taaagtaagg
     4681 aaacaagaat aaatttcaat gaaggaagag ataagatgcg aaaagtagca cctattttga
     4741 agccaatcgc tgtattaaca atctcgacga ttgtcgcgac tactacaggt attgtgaata
     4801 gttccagcta ccgtgcacca agttcaagtt ctagtactgt agaagaaaca acaccaacta
     4861 cttcagtctc gacatctgaa ccaacaacaa cggaaagttt gaatgaaacc actacgacaa
     4921 gtacatggga cgatacaaca actagttcgt cgtatgatga gacatataca actgacagct
     4981 caagcgaaac aagtaatacg gtaccagatg gcactccatc ggattcaacc aatccaagta
     5041 caagtccgga tacaagtgct agctccgaaa cgataccaag cagtacgact gtagatgata
     5101 acacgcaaaa tgatgatgca gcacgtacag cacagcaaaa ccaagattca ggaaataccc
     5161 aaacgtataa ccagcaagca ggtgattctc aatgacattt ttactagacc ttatcaacgg
     5221 ggtgcataaa ttccttggct atttagacat cagtcctaaa tatcttaacc gtggatatac
     5281 gattttaagt gtgattccca cgctttattt attgagaatt gtttatggac tttggcaaaa
     5341 ccaaaactat cttcaatttt tcttatatgg acttgcattt ctagtgcttg tttattttac
     5401 tgttttaaat gtattttatt actttttaga taaaaacacg aaagccgatg tcactcagct
     5461 ttttgtgaag tatttacctg atgaagcgtt caacatccag acagaagaat cgatagataa
     5521 tggaaattcg atcgataaag tgaatacaca agaggtcatg gtttcttatg acgaagatta
     5581 tcaattgaaa ttagccgaga atatgcggta tttgattgga aatggtgaga tcaaaacaaa
     5641 tgaactcggt cgcatggatg gatttctggt cgatagaaat acattgtatc cttattacta
     5701 tgtcaagaaa acaggagaga aagagtatgt tctaaatatt ggacgaagct actcggacat
     5761 ggaaaaagta gggaccattc gacttgactc aacagaagac acattggaac ctgtcggatt
     5821 gttcatcgtc ggcggtgatt ttgtcaaaga cgggttacgc tatcatgagc cttatcgtct
     5881 gaaattgatt gcaaaaaagg cacagtcgca ggaagaacca gtggtttata gtcgtagcca
     5941 aagaagaaga cattcataat gtgatgaaac gccgtgtatc agtggttgcg ataaaaacgt
     6001 ttattcggaa taagggggat gtgacacgtc ttttgtcacg ttcctttttt atataacgag
     6061 aaagataaga cgaacacatc acttattctt tcctttcatt tataaacatt gtagaataaa
     6121 gaaagtacaa cgaagtattg tgaaaaaacg taataaataa cgaggaaaat acaagtagga
     6181 ggtaagctgt gaaagaattt ttgaaaaatt gggggatttt gatccttgta ttagcggcta
     6241 ttttattagc tagagtgtac gtgtttacac cagttactgt aaacgggcat tcgatggacc
     6301 ctacattaag tgatggacaa cgattgattt catctaagat ctcaaactat gaacgaatgg
     6361 atattattac tacaaaggaa ccgggggatg aagaacggat gattgttaaa cggatcatcg
     6421 gtatgcctgg agatacagtc aagatggaaa atgaccagtt gaccattaat ggcaaaaagt
     6481 atgatgaacc ttatctggat gagttcaaaa aagaattttc agaagataag ttacaaggag
     6541 aatatgctta tagctcagga ttccaagcac aagcagaaag ttcatccacc tttacgagtg
     6601 actttgaata tactgtgcca aaaggaaagt atcttgttct aggcgataac cgcttgattt
     6661 caaaagacag ccgaatgttt gggttagtcg acaaagatat gatccaagga aaagttgttt
     6721 tccgttattg gcctctttca gaaattaaaa tcatgtgatg aaaaaataaa aagaagctgt
     6781 gacatgtttt ttgtcacagc ttctttttcc gaaaattcct tcttatttct tggagttgct
     6841 cacttttttt gcaatctcat tttaagcttt ttggctgatg gataagaaaa gcgaagcgtt
     6901 tttgttcata aatagctttt ccccacgatg gaaggtatga ctagtagcag agacaaagcc
     6961 cgttttttcc aatcgattat tcaattcttc ttggctaaag ccattatgga ctttcggatg
     7021 actaatttgt tcattcttgt caaagtctac gatgatcaat tgaccgccag gagctaaaat
     7081 ttcataaagt tttgtaagaa tattctccgt atcaggaatg tgtaaaagta caagtgatag
     7141 aaggatgaga ttagcttgga cagcaggaaa ttcgacagaa gcatcagcgt ggatcgttcg
     7201 gacatttttc agatctgctg cttggatctt ttcttctgcc attttcagca tagtttctga
     7261 agcatcggca atgatcagtt ctttgaatct ttccgcaagc ggtaaactaa caagcccagt
     7321 tccgcctccg taatccagca ggactttttg gtggctgtcg cgcggtagat aagtagttag
     7381 ttcttcgcga ataatcttgg ctaatgcttg acggtcaggg gaatcataat ggttagcgat
     7441 ggaatcaaaa atattttttg tcatcaaata gctccttacg taaaactttg aaattttaac
     7501 ttattatagc acgtgaaaag aaagaaagca tgctatactc aattcaaaaa gggagaatct
     7561 agaaagaata tgccgatatc gtttgtaaaa gatagagagg aaaaaggaaa gtgtgtccga
     7621 gaaatattac tagacttgcc tgagtggttt gggcttcctg aatcaacgga aaaatatatc
     7681 gaggaatcaa gtaaactacc tttatggtgt gaaaaaagaa aagaagaata tttaggcttt
     7741 atcactcttt cacagacaag tgaagatact gccgaaatct attctattgt atgggaataa
     7801 aaaaagacta tcatcaccaa ggattaggaa caaaactttt taaagaagca gaaggttacg
     7861 cagcaaaaca ttacaaatat ttgcaagtga agactgtaga cgaggggcac tactctatat
     7921 atgatcaaac tatttgtttc tacgaatcat taggattttc aaggttggaa gtttttccaa
     7981 atttatggga tgaatggaac ccatgtttag ttcttgtcaa aaaattggaa caaaagtgag
     8041 caatagattt aatactaatc tgtgcttgat gaaaattctc actgtaaaaa atgctgaaaa
     8101 tcaagtataa taccatagga acaaactgga aggtgaaaaa gagcatggca caactatttt
     8161 ttaagtatgg cgcaatgaat agcggcaaga cgatcgagat cctaaaagtc gcacataatt
     8221 atgaagaaca aaataagcca gttgtcttaa tgacaagcgg tattgataca agagatggtt
     8281 taggcgttgt ttcaagccga atcggactga aacgtaatgc gatgccgata tttgaagaaa
     8341 cagacgtatt tgacgtaatt cagaaaatgg aaagcaaacc ctattgtgtg ctgatcgatg
     8401 aagcgcaatt cttaaaaaag aaacatgtca ttgcttttac gagaattgtg gacgaattgg
     8461 atattccggt tatggcattt ggtttgaaaa atgattttcg aaacgagtta tttgaaggat
     8521 caaaatattt attgctttat gcagacaaaa tcgaagaaat gaaaacaatc tgttggttct
     8581 gccacaaaaa agcaatcatg aatttacatt atattgatgg aaaccctgtt tatgaaggcg
     8641 atcaagtgca aattggcgga aatgaagctt attaccccgt ttgtcgaaaa cattactttc
     8701 acccatcaat gattacagaa ggagaataag catgtacgat caattacaag caattgaaga
     8761 ccgttatgaa gagcttggag aacttttaag tgacccagaa gtgatttctg atacaaagcg
     8821 ttttatgcag ctttcaaaag aagaagcaaa cacgcgcgaa acagtcgaag tttatcgtcg
     8881 ttataaagaa gtagtacaag gaatcaccga tacagaagaa ctgcttggtg aaaaactgga
     8941 tgatgaaatg gctgaactag caaaagaaga attatctgaa ttaaaaaaag aaaaagaagt
     9001 cttagaagaa aaaatcaaga tcttgctttt accaaaagat ccaaatgacg ataaaaatat
     9061 catcatggaa atccgtggag cagcaggcgg agacgaagca gcattattcg ctggcgatct
     9121 tttcaatatg taccaaaagt atgctgaagc acaaggctgg aaaacagaag tgatggaagc
     9181 taacgttacc ggaatcggtg gatataaaga agtgatcatg atgatcaccg gagacaacgt
     9241 atattcgaaa ttaaaatatg aaagtggtgc gcaccgcgtc cagcgtgttc cttcaactga
     9301 atcgcaaggg cgtatccata cttccaccgc aacagttgtc gtgatgccag aagctgaaga
     9361 agtagagatc gatattgaag acaaagatat ccgtacagat atctatcatg cttcaggtgc
     9421 tggcggacag cacgtcaata aaacagcatc tgctgtacgt ttgactcact taccaacagg
     9481 gatcgttgtt gctatgcaag atgaacgttc tcaaatcaag aaccgtgaaa aagcaatgaa
     9541 aatcttgcgt gctcgtgtgt acgataagat ccagcaagaa gcacaaagcg agtatgatgc
     9601 caatcgaaaa tcagcagtcg gaactgggga tcgttcagaa cggatccgga cgtataattt
     9661 cccgcaaaat cgtgtgactg atcatcgtat cggattaact atccaaaaat tagatcagat
     9721 tttagcaggg aaattagatg agatcgttga tgcattgatc atgtatgatc aaacatcaaa
     9781 actggaagct atgcaaaatg ggtaaaatga cctatcgaga agtcctgtca cgggcttctt
     9841 cttttttaga ggatcatggg aaagaagcat atagtatcca atttttattt ttggaacgaa
     9901 aacaatggaa aaaacttgat tggctccttc atatgaacga agagatttca gaagaagaac
     9961 agcggttgat cgaaaccgat ctgcgacttc tgcttgcaga tcatccacct cagtatttgc
    10021 taggatatgc agatttttat gatcatcggt taaaggtaac tgaagcaaca ctgatccctc
    10081 gaccagaaac agaggagtta gtcgaatggt gtttagacga gacgcctggt gtaccgttag
    10141 aagtgataga tattgggaca gggactggag caattgctat tagtttgaaa gcagcaagaa
    10201 aaaattggca tatatctgct gtggatcttt cagaagaagc attagaagta gcgaaagaaa
    10261 atgctcagaa agaaggaacg aaaatcagtt tttatcatgg agatacatta ggaccagtaa
    10321 tggatcagca atttgatgtg attatttcta atccgccgta catcagtcgg aatgaatggg
    10381 aactgatgga tgaaagtgtc cggtcattcg aacctaaaat ggcccttttt gctgagaacg
    10441 atggattagc tatttatgaa aaaatagcag ttgaagcacc aagcgtacta aaatcaaatg
    10501 gaaagatttt tttagaaatc ggatttcgcc aaggtgaagc agtaaaaaaa atatttcagc
    10561 aagcatttcc agataaaaaa gtggcgataa aaaaggatct ttttggcaat gaacgaatga
    10621 tccaagttta tggttgaagt tgtggatgaa aaatttttta tttgtttttt tattataaaa
    10681 aaacacttat ttgacaagga tgtaaacgac tttttggttt atcaacaagt ttatccacta
    10741 ttgacaaata gattatccac aaacagtgga aaacttgagg ataaactcag acaaaataag
    10801 aatgtatgaa aaaggggttg tggataaact gtggaaacga aacgatatga tgaaacagaa
    10861 ttgcaagagg cagccgaagc actgaaagaa ggagaacttg ttgcttttcc taccgaaaca
    10921 gtatacggat tgggcgccaa cgctctttta ccaaacgctg tcaagaaagt gttttctgta
    10981 aaagggagac cgcaagataa tccattaatc gtccatgtcg cctcttttga acaagtaaaa
    11041 gagtatgtgg acaactttca tccggcagca gagaagattg tgaagaactt ctggcctgga
    11101 ccattaactt tgatatttaa gatcaaaaaa gacactttgc cctcagttgt gacgggagga
    11161 ttatccacag ctgcatttcg tataccggat aacaaaaaaa cactagaagt aatcgaactt
    11221 tccggtgttc cgcttgtcgg tccaagtgca aatacgtcgg gaaaaccaag tccaacgact
    11281 gcagatcatg tatatcatga tctacaagga aaaattaccg ggattattga tgatggagct
    11341 acgcgaattg gtgtagaatc cactgttctt gatttaagtg atccaacagc catgcctatg
    11401 attttacgac caggtgcagt aacgaaagaa caaatcgaag cagtcatcga atctcctgtt
    11461 gctattgatc agcatcttgt aaaagaaaat gaaacaccaa aagcccctgg gatgaaatac
    11521 aaacactact cacccgatac acgtgttctg atggtcagag atggcgattg gtcaactgca
    11581 gttcaatggg caaaaaataa gaagatccgt gcaggtgtca tagcaagccc cgaaattgcg
    11641 gatcaagtgc ggacagatac tgcagctgtt tatatgtata acgacaactc agtagaagcg
    11701 gcggctaaag gattgtttgc aggtctaaga ggattagatg aaccaacact tggattagat
    11761 ttgatttttg tacaagtgta tccagaaact ggattgggga atgcttacat gaatcgcttg
    11821 aaaaaagcag caggacaaaa ttattttgaa aaatgatcaa ttggttattg tcgcattctt
    11881 ttagtatgat acaatgctat caagtaaatc ttctatcagt gaacgaaaaa ctaagcgtaa
    11941 ctaatcgtaa tagaagagat acgaatattt ggcaatttgt ttttgatggg aaaaataaaa
    12001 ttgtaggaaa ataaaaagga gctgttgttt gtggtagatt acaaaacgtt tgaccctgat
    12061 ctttgggctg cgatcgccaa agaagaagaa agacaagaac ataatttgga gctgattgcc
    12121 tctgaaaact ttgtctccga agcagtgatg gctgcacaag gaagtatctt gacaaataaa
    12181 tacgcagaag gttatccagg gcatcgctat tatggtgggt gtgaatttgt cgatatcgtt
    12241 gaaaatttag cgattgaccg tgcaaaagaa ttattcggag cgaaatttgc gaacgtccag
    12301 cctcactccg gctctcaagc caacacggct gcttatctcg ctttggtaga accaggggac
    12361 acgatcttag gaatggatct ttccgcaggc ggacatctaa cacatggatc accagtcaac
    12421 ttcagtggca aaacctatca ctttgttgct tacggtgttg atccgacaac agaagttatt
    12481 gattataatg tggtacgtat tttagctaga aaacatcaac caaaattgat cgtagcaggt
    12541 gccagtgctt acggacgaac gatcgacttt gcgaaattca gagaaatcgc agatgaagta
    12601 ggcgcaaaac tgatggtaga tatggcacat atcgcaggat tagtagcagc aggacttcat
    12661 cctaatccag ttccttatgc agatatcaca acgactacca ctcataaaac attgcgtggc
    12721 cctcgaggcg gcatgatttt gacaaacgat gaagcattag caaaaaaaat aaatagtgcg
    12781 gtatttcctg gtattcaagg cggccctcta gagcatgtga ttgcgggtaa agcagtagca
    12841 ttcaaagaag cattagatcc tgcatttaaa gaatatagtg aacaaatcat cgccaatgca
    12901 aaagcaatgg tcaaagtttt caatcaagca attggaacca gagtcatttc aggggcgaca
    12961 gataatcatc tgatgttgat cgatgttcgc gagttaggta taaatggaaa agaagcagag
    13021 agcattttgg acagtgtgaa tatcactgta aataaaaact cgattccttt tgaaacacta
    13081 agtccattca aaacaagcgg tattcgaatc ggcacgccag caatcactac tcgcgggttc
    13141 aaagaagaag atgctgtaaa agtggctgaa ctagttgtca aagcattaca agcaaaagat
    13201 gataacgctc aactagacga agtcaaaaca ggtgtacgcg aattaactga aaaatttccg
    13261 ttgcataaaa aataaaaatt taaattccat tagtaaacag aaaagttcaa tgttgggctt
    13321 ttctgtttat tttttgggaa aacggattct ttgacaatat cggtgatgct ctctagtata
    13381 tgaggtgtaa ttttagtctt cacactaaat ctcagggagg aaaagtcatg actgatcgta
    13441 tctcgttaga aaaggaagca gtagcattca gtgatgcaaa tgcgcctcat ccgcgtatct
    13501 atgaactagc accggaagaa ggaagagttt tactagaaga agtacaagaa tcacctgtga
    13561 acaaatattc cgtcgatatt gaagacttga tggtggatac tggcgaatgg ggaaaactca
    13621 atattcgtct cgtacgacca gaagggaata tggataaatt accagttatt ttttatattc
    13681 atggagcagg ctgggttttt ggcagtgctc aaacccatga taaattgatc agagaacttg
    13741 cggttcgtac gaattccgtc gttgtctttc cagaatatag tcgatcacca gaagcaaaat
    13801 atccaacagc aattgaacaa aattatgctg tgttgcaaca gctgaataaa ttagccaagt
    13861 caaaaaatct ggatctcgag aatctaatcg ttgcaggaga ttcagtaggt ggtaacatgg
    13921 ctacagtcat gacgatctta acaaaagaac gaaagggcct cccaatatcc aagcaattgc
    13981 tgtattatcc agtcacaaac gctgattttg atacagattc ctatcatgaa ttcggagaaa
    14041 actatttttt gacaagagaa ggaatgatgt ggttttggga ccaatatacg acgaatgaag
    14101 aagagcgtgc tgaaatcaca gcttcgcctt tgagagcaaa tataaatgaa ctaaatgatc
    14161 ttccttcagc aatgatttta acgggagaag cggatgtatt aagagatgaa ggtgaggctt
    14221 atgcgcgcaa acttcgtgat gcaggggttc ctgttacaca agtacgcttc caaggaatca
    14281 tccatgattt tgtcatggta aatgctatgg atcagacaaa ggcaacgcgt gcagcaatgg
    14341 cattatctac ttcttggatc aagaaaaaag aataagcttt gcttgtccgc aggaaaactt
    14401 cttttcaaaa acggattttc ttaggaaaaa tggcgagttt gactagtcaa tcacttgatt
    14461 ttgttttaca attaaaaaga aacgacagaa caaaggagag cttaaaaaat gggcaaattt
    14521 caagtgatcg atcatccatt gattcaacac aaactaacta ttattcggga caaaaactgc
    14581 ggaacaaagg tttttcgtga agtagtcgat gaaatcgcta tgttgatggc atatgaagta
    14641 tcacgggata tgccattgga agacatcgtg atcgaaacac cgatcactga aacaacacaa
    14701 aaaacgttgt ctggtaaaaa agtagcgatt attccaatct tgcgcgcagg tattggaatg
    14761 gttgacggta tcttggaatt gattccagct gctaaagtgg gacatatcgg tctttatcga
    14821 gatgaagaaa cattggaacc acatgaatat ttcgtcaaac ttccagaaga cattgatgca
    14881 cgtcagttgt ttgtagtaga tccaatgttg gcaacaggcg gatcagcaat catggcaatc
    14941 gatgcattga aagaacgtgg cgcgtcgaac attaaatttg tttgtttggt agcagcacct
    15001 gagggagtaa aagctttgca agaagcgcat cctgatattg acatctatac agcttctcta
    15061 gatgaaaaat tgaatgaaca tggttatatc gttcctggac taggggatgc gggtgaccgt
    15121 ttgtttggta caaaataagt aaatacgaaa aagggtgtga cacaactttt gtcacatcct
    15181 tttttggtca tttttaacgt ttgatgcaga acagcttttt cacagctttc gtttgcatat
    15241 aaaaaaacca tcccaaaaat gcagagatgg tcaaattcaa atctttttta gttctttgta
    15301 gttaacgatt actacaccct tactttgcgt catcaagttc gcattttctg cagtttcatc
    15361 aatctcaaca acagcggaat ttttcaattg ctttgaaatc gttccagttt gcgacttgcc
    15421 gtgaattgtg aacattacgt gagtattgat cttgaatttt tcaccaattt ccgatggtac
    15481 gatagttgta tcaaattttc caaaagttgc cattttcata aaacctgcct ttcacatcaa
    15541 ttataccacg ttttatgaaa aatatcatga aattaaacta attttccatc ggactcttta
    15601 caatcagcca aatagagtat ataatttctt ttggataagg ccagcttagc tcagttggta
    15661 gagcaacgca ctcgtaacgc gtaggtcgta ggttcgatcc ctatagctgg cattaaaaga
    15721 gaagacagat atatcaagga tttttaatcc attgatacat ctgtcttttt gtttaaagga
    15781 aataaagtat gctttcttag caatagagca tgtattattt ctataaaaat ttaggagaat
    15841 aaaacgacta gcaatatagc tgtgaaatca aatatcacat gaactgccca acttgtaatg
    15901 atcgagttgc ttttaatggc agcttggttg aataacaatc ttgccacccc ttgtatcaaa
    15961 aggatgtgtg ctacagtgtg taaaatattt ccattatcat aagtactgta atggataaat
    16021 ccaaagatga ttgctgacaa gaaactagcg acaggaatgc tgaacttact agaaaaaata
    16081 gcgagaaaat acactgagaa caattcttct ccaagtatca tgataggcaa aataataaat
    16141 aataacgggc tgaaagcctc atttattgga ttgcctgtta aaccccattt caaaatttgt
    16201 tgcaaaaata ccgagactgc ggcactgatt actacattga taaaaaaata tttggctata
    16261 tttttgacag gagcgacagg tttcgagaac atttttttcg tatctttttt accaaaaaca
    16321 agtgtaaatg ctataaatcc agtgatagtt aaagaaaaaa tgctaataat ggatggcgca
    16381 aatagcaacc ctaacaaacc gagtacaacg acaatagtgc ccaatatccc ttttttaagt
    16441 tctatttttt ttcctaaaat gttattcatg ataataactc ctactattca gtttttgaag
    16501 atagagtcag tatacatgaa gagttaaaat aggcaatgtt tttttatgga tatttgtatt
    16561 gctaaaaatt tatcttatat attcattttg attatactac ttttatttgt aaaaattttc
    16621 tagcaaaacc aagtgcttgc aactgattaa gcgtacaatt ttttagatgt tccgagcgta
    16681 cttcaatcat ttcaaaggag ttgctactta aatcgatatt ttttaaatca gtatcaaaaa
    16741 agctagcatt ttctaaatca ttagtagaaa acattatttt agtgagggaa cagtctacaa
    16801 aatctgcgct tgataagaca gagtgctcaa aggtactttc ttttatgttt gaaccagata
    16861 aatttacata gtccatttta caattctcga ataacgtgtt cccaatataa ctttccatca
    16921 atttagtacc tgttaattta caattgacaa atttcacgcg atgcaacatg ctttttgtca
    16981 atatacagcc tgaaaaatca cagttttcga atatacagtc ggtaatctcc attctttgaa
    17041 aatcattgga agcaaaaaat gcatacttga aaatagattt gtaaataatt ggatggcaga
    17101 ttatttggtt ttcgatgacc tctttttgtg aaaggatatt atacagttca aaatcttcgt
    17161 tttcatagat atgaaaaaag gatcctttat tcaagcttgc ttcattaact ttaggaaaaa
    17221 tcatatttaa ttacctccat tttcataaag tcatattaac aaaagctaaa ccaacttaca
    17281 ttagcagaaa atgagatagg ttttcgcctt tttcgataac gatagtgtcg ctatctcttg
    17341 aaacgttctt agaattgccc tgagagacac aaattaggcg taattctaat aaaaatctta
    17401 gaataaaaat ttttactgat ttaatttaga atgtttttaa aataaaaatc gttgccataa
    17461 cagtgtattg aaaggtttta acaatccagt aatgatttaa aaaaatctag atgtagcgta
    17521 tactttgttt atagaatgta gtgggaagaa atatattttt gggaggaaat cgtgatgtct
    17581 aaaagtgaag aaatagaaaa catggtacta aaattatatg atctcgtttt aaatccagag
    17641 atcaaagaaa aggaacgtat acttttgata gacgcaaaga caggcttaga aaaaggtcag
    17701 tattatccta aagtcataaa taatttggaa cgtagtttac gtccgttagc aatcagggga
    17761 gaattgtcta aaccagtatc accgttttat atggagatat caacaatagg aaaatttgaa
    17821 aaagaattag gtcgaggaat ggcttcggca ccgattactt ttgggcattt ataaaaacga
    17881 gttattcatc tcaatgaatt aaaaagtata gtcaatctca taatgcaggc taggttcatt
    17941 tatttgaaca gagtctgtat ttttttattc taaataaaga aacagatgtt tctttatatt
    18001 gtattttatc ctttaattaa cacaaaaaca gctagtataa actaataatg ttaattaaaa
    18061 gtgtatttta tgaaactatc gttatttatt aatgttgcat ttttgtaagt taaaaattgg
    18121 aaatatagat tgaaaaaatg aaagcaataa gtaaaataaa atgaatatat gcgaaaaaat
    18181 aatttacatt aggaaaaacc ttgtaaaaac agttttttgg catatttatt ttttataaat
    18241 atttgtaaaa taactatcaa ttttttagtt aaaagattgg aaatgagtgg gggaggaatt
    18301 gaagaaagga tttttgagag atgatatagt aggttttatt tttagttaaa ataggggaga
    18361 tagaaaaaat gaaaaagtgt agaaatatta ttttatcaat gttatttatt attactaata
    18421 taacatcact gattcctgtt catgtctatg cggatgcagg cagagatatc agcagtaatg
    18481 tcacttcgtt gaccgtggac ccaacgaata ttacagatgg aggaaacata aaagtaaaat
    18541 tctcgtttga cgaaaaaaaa caaaatatcc aaccaggtga ttatctttgg atcaattggc
    18601 caagtgaagg aaatattcgc ggtgaaggat tccaaaaaga aatcccatta atgattgaaa
    18661 ataaaaatgt tggaacttta acggtcagaa aagattctgc gcaggttgtg tttaatgaaa
    18721 atattaaaaa cctagactca gtagaaggtt gggggcaatt tgagatacag gccagaaacg
    18781 taaccgatac cggtgaagaa aatacgggac cattcaccgt gacgagcggt gataaaacag
    18841 ctactgtaaa tgtgacaaag ccagcttctg gttcttcttc tagcgttttt tattataaaa
    18901 caggagacat gctacctgaa gatactaagc acattcgatg gtttttgaat attaacaata
    18961 acggcactta tgttgaacaa ccagttaaaa tcagtgatga aatccaaagt ggacaaagat
    19021 tagatccaag tacgtttgaa attaatcaaa tacacttagg tgaacagaag gtttatagag
    19081 gagaagaggg aatccaacaa tttttacaag atttccccag cgctacattt aatttttcag
    19141 taactgataa ttacattgaa ataactatac caaaaaactt tgtcaatctt aggaaaataa
    19201 tggtgagtta taagacgatc atagaaaacc cagaacaaat aaattttgag aatcattcgg
    19261 aagcttggtt taaagaattt aataaacctg ctgttgatgg agaaagtttc aatcatacag
    19321 ttaaaaatat ttcagcttct ggtggagtca acggtacagt tagaggcgaa ttgaagattt
    19381 tcaaatatat caatgacaca gagattggta tcccaaatgt gactttcgaa ttaagacgag
    19441 cagatgaaca gcctattcaa ggacagtcta gtatcctgtt gacttcgaat gaacagggag
    19501 aagctagtat caaaggcctg caagtaggag actatgttgt aaaagaaaaa gaagcaccca
    19561 attggattga ttttgatcca ctaagttcaa atgaactcaa attctccata aacgaaaatg
    19621 atacagaggg agtcagttta cctatttata acaagaaaaa ggttacgaac atcacagcaa
    19681 ctaaaatttg gaacggtgga accactccaa gaccgagtat ttattttaaa ttattcagag
    19741 caagtacaaa caattgggaa ccagttcctg atgcagaaac aaaacgatta gacaatggaa
    19801 taacttcagt gacttgggaa gatatccaac agtatgatga tagtggaaac gaatatacgt
    19861 ttaaagtgca agaagtagat caaaacggaa atgattatgt cccttcaggt tatcaaaaaa
    19921 ttgaaaacgg gctagtcgtt acaaatgaga ataaagaagt catttcagtc tcaggacaaa
    19981 aaacatggga agacaatgag aaccaagacg gcaaacgacc gacgacgatc acagtaaatc
    20041 tattagccga cggtcaaccg atccaacaca aagaagtaag tgaaaaagat gattggcgct
    20101 atcgtttcac aaatctgcca aaatacagag acgggcaaga aatcatctat actgtaacgg
    20161 aagacaacgt gcccgaatac agtacgacga ttgacgggta taacatcaaa aacagttata
    20221 caccggaaaa aacaaatata tctataccag gtagccatat aacaccgtgg aaaaattttc
    20281 cagagaagac agagagagag aaaattagaa atctattctc tcaaaaattg caaatatttg
    20341 ctcatacagg agaatccagc gaacaaagaa gcaattatat tagtaagaga gctgtaccta
    20401 aacagataac aaataaatca aaaagcattc ttcctaaaac aagtagtaaa aaatctagtt
    20461 ttgctgtaat cattggttta ttgatagtaa ctatctgtgc tgggatattc ttacaaaaat
    20521 ataaaaaagt ttagttagaa tataatattt tttgttaaat atgactgaaa tcaaaagaat
    20581 aaaattccga tttataatcg aaaaaagaag catttttata tatgcttctt ttttcgtaga
    20641 atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt tcagcgttca
    20701 tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt gtgctaaata
    20761 aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca aattttcata
    20821 aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat ccataatcaa
    20881 cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa tagcagcatc
    20941 gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag tcactgtacc
    21001 tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata agcgcttttc
    21061 ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca agatgacaag
    21121 atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg gaccaggtag
    21181 ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt gaaaagagat
    21241 acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca tcgcgcatca
    21301 ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa taatcatgta
    21361 aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt aggtgttttt
    21421 agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat cttggagtcg
    21481 attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg gtcgttgcta
    21541 gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg gaaccacgtg
    21601 attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa cggcaacgac
    21661 tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt ataatacgtc
    21721 ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag atggagtatt
    21781 tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa ttatttttat
    21841 ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg tctttgcatt
    21901 ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga acaccgctgt
    21961 tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt tttgatcgtt
    22021 tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca gagatctttc
    22081 gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt ttgcaattaa
    22141 gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt gttttacctt
    22201 ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat atattaggtt
    22261 taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact ttactgcctt
    22321 tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc ataatgaaac
    22381 aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat atgattgaat
    22441 tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg aatctaatca
    22501 ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa accacgcttt
    22561 tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta gacggtgtac
    22621 cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta ttccaagact
    22681 tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca aaaatggtat
    22741 tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct tcattaggga
    22801 ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa cagcgggtag
    22861 cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa ccaacaagtg
    22921 ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg aaagaagatg
    22981 gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc ggtgatcaat
    23041 tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa aaaccagttt
    23101 tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca aaaaaacttc
    23161 agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt tagacgatac
    23221 atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg atattgattt
    23281 agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca ttgactggtc
    23341 aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg gctattccaa
    23401 aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa acgatcaagt
    23461 gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata ataagagatt
    23521 aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag aagtattaaa
    23581 agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag cattgatcga
    23641 tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag actattattt
    23701 gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg agagtgagga
    23761 ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca atgaagccct
    23821 agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt ttggcgaaga
    23881 tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca cagtcccttt
    23941 ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa tggcaggctc
    24001 acaacctctt ggtaaacggt gttcaaaagc cagttt
//
LOCUS       DACDXE010000041        23826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.041, whole genome shotgun sequence.
ACCESSION   DACDXE010000041 DACDXE010000000
VERSION     DACDXE010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.041"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(54..788)
                     /locus_tag="IWM23_001656"
     CDS             complement(54..788)
                     /locus_tag="IWM23_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAP9884291.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="IWM23_001657"
     CDS             1115..1525
                     /locus_tag="IWM23_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAP9884292.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="IWM23_001658"
     CDS             1518..2219
                     /locus_tag="IWM23_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAP9884293.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="IWM23_001659"
     CDS             2219..3805
                     /locus_tag="IWM23_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9884294.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="IWM23_001660"
     CDS             3824..4045
                     /locus_tag="IWM23_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAP9884295.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="IWM23_001661"
     CDS             4042..4803
                     /locus_tag="IWM23_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAP9884296.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="IWM23_001662"
     CDS             5183..5692
                     /locus_tag="IWM23_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAP9884297.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="IWM23_001663"
     CDS             complement(5767..6306)
                     /locus_tag="IWM23_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAP9884298.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IWM23_001664"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="IWM23_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAP9884299.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7373..8170
                     /locus_tag="IWM23_001665"
     CDS             7373..8170
                     /locus_tag="IWM23_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAP9884300.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            8198..8833
                     /locus_tag="IWM23_001666"
     CDS             8198..8833
                     /locus_tag="IWM23_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAP9884301.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="IWM23_001667"
     CDS             8853..9626
                     /locus_tag="IWM23_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9884302.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="IWM23_001668"
     CDS             10034..10831
                     /locus_tag="IWM23_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAP9884303.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="IWM23_001669"
     CDS             10809..11222
                     /locus_tag="IWM23_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884304.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="IWM23_001670"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="IWM23_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAP9884305.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="IWM23_001671"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="IWM23_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAP9884306.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /locus_tag="IWM23_001672"
     CDS             15999..16559
                     /locus_tag="IWM23_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAP9884307.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IWM23_001673"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="IWM23_001673"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAP9884308.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="IWM23_001674"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="IWM23_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAP9884309.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="IWM23_001675"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="IWM23_001675"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAP9884310.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="IWM23_001676"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="IWM23_001676"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAP9884311.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="IWM23_001677"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="IWM23_001677"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAP9884312.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="IWM23_001678"
     CDS             21906..22484
                     /locus_tag="IWM23_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAP9884313.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="IWM23_001679"
     CDS             22536..23018
                     /locus_tag="IWM23_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAP9884314.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="IWM23_001680"
     CDS             23135..23509
                     /locus_tag="IWM23_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884315.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7782 a         4285 c         5114 g         6645 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaag
//
LOCUS       DACDXE010000042        23637 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.042, whole genome shotgun sequence.
ACCESSION   DACDXE010000042 DACDXE010000000
VERSION     DACDXE010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.042"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..279)
                     /locus_tag="IWM23_001681"
     CDS             complement(<1..279)
                     /locus_tag="IWM23_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAP9884316.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFN"
     gene            complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IWM23_001682"
     CDS             complement(304..735)
                     /gene="ruvX"
                     /locus_tag="IWM23_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAP9884317.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(732..998)
                     /locus_tag="IWM23_001683"
     CDS             complement(732..998)
                     /locus_tag="IWM23_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAP9884318.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(1253..1978)
                     /locus_tag="IWM23_001684"
     CDS             complement(1253..1978)
                     /locus_tag="IWM23_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAP9884319.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(2339..2409)
                     /locus_tag="IWM23_001685"
     tRNA            complement(2339..2409)
                     /locus_tag="IWM23_001685"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2375..2377),aa:Gly,seq:ccc)
     gene            2526..3278
                     /locus_tag="IWM23_001686"
     CDS             2526..3278
                     /locus_tag="IWM23_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAP9884320.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            3294..3773
                     /locus_tag="IWM23_001687"
     CDS             3294..3773
                     /locus_tag="IWM23_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAP9884321.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(4024..5604)
                     /locus_tag="IWM23_001688"
     CDS             complement(4024..5604)
                     /locus_tag="IWM23_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAP9884322.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(5597..6340)
                     /locus_tag="IWM23_001689"
     CDS             complement(5597..6340)
                     /locus_tag="IWM23_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884323.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(6516..7292)
                     /locus_tag="IWM23_001690"
     CDS             complement(6516..7292)
                     /locus_tag="IWM23_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884324.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(7540..8376)
                     /locus_tag="IWM23_001691"
     CDS             complement(7540..8376)
                     /locus_tag="IWM23_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAP9884325.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(8387..9316)
                     /locus_tag="IWM23_001692"
     CDS             complement(8387..9316)
                     /locus_tag="IWM23_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAP9884326.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            9508..9861
                     /locus_tag="IWM23_001693"
     CDS             9508..9861
                     /locus_tag="IWM23_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9884327.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(9935..10591)
                     /locus_tag="IWM23_001694"
     CDS             complement(9935..10591)
                     /locus_tag="IWM23_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAP9884328.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            10817..12751
                     /locus_tag="IWM23_001695"
     CDS             10817..12751
                     /locus_tag="IWM23_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9884329.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(12837..14060)
                     /locus_tag="IWM23_001696"
     CDS             complement(12837..14060)
                     /locus_tag="IWM23_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAP9884330.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(14054..15952)
                     /locus_tag="IWM23_001697"
     CDS             complement(14054..15952)
                     /locus_tag="IWM23_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAP9884331.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(15946..17064)
                     /locus_tag="IWM23_001698"
     CDS             complement(15946..17064)
                     /locus_tag="IWM23_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9884332.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(17147..17980)
                     /locus_tag="IWM23_001699"
     CDS             complement(17147..17980)
                     /locus_tag="IWM23_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884333.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(17973..18890)
                     /locus_tag="IWM23_001700"
     CDS             complement(17973..18890)
                     /locus_tag="IWM23_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884334.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(19117..20241)
                     /locus_tag="IWM23_001701"
     CDS             complement(19117..20241)
                     /locus_tag="IWM23_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9884335.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            20424..21332
                     /locus_tag="IWM23_001702"
     CDS             20424..21332
                     /locus_tag="IWM23_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884336.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            21473..23086
                     /locus_tag="IWM23_001703"
     CDS             21473..23086
                     /locus_tag="IWM23_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAP9884337.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         7372 a         4617 c         3949 g         7699 t
ORIGIN      
        1 attgaatact tcttcaatca tgtcccattc tgcgtctgtt tcgatttgtt ctaactcgcc
       61 ttctgttcca tcttcgtttt cgatgtatgc ataagcttgt aattctacat cttcatcttc
      121 tgaaacacca gcaggatata aaagaacata gttacgtcca aattcttctt gtccgtcaac
      181 tgttagtagg atttcatata atgtttcatt tccttggtca tccaccaatg tgatgtgttc
      241 gtgtccttca tggtcgtggg aatggttatg ttctgtcata agattacctc ttttctttta
      301 ttattaaaat aattgcttac ctgagccgtc taaataattt tgtaagatca tcacagctgc
      361 taatttatcg atgacttttt ttcgtttggc tcttgaagta tttgcctgct ctactagcat
      421 acgttctgcc tgaacagtgg ttaatcgttc atcttgataa tcaactggca aggagaatag
      481 ttctttgatc ttttctccgt atgacatgga agcttctgcc cttggtccga tggaattgtt
      541 catattttta ggtaatccaa ctacaaaccg gtctacttca tattgtgcta ctaattcctt
      601 caaccgatca aagccaaact cttcttctgc ttcattgatc cgaatgatct ctactccttg
      661 agctgtccag ccaaaggggt cactgatagc aacacctact gtcttagaac cgacatccaa
      721 gcccattact ctcataaatt cactccatga tttgagaggt agtccttgac aattacttcc
      781 atgatctcat cgcgttcgtg acggcgaatc aaatttcgag catcacgata acgaggaatg
      841 taggccgggt ctccagaaag caagtagccg acaatctgat tgattggatt gtagcctttt
      901 tcttccaaag cccgataaac aatcgctaat gtttcgctca cttctttttt gcgactatcg
      961 tcaaaaccaa aacgtacggt ttcatctgta aaacccatga ttctacacct ctttcccgca
     1021 aggtaattcc agcttacctt ctacaacctt attttactcg aagaaaggga aaactacaaa
     1081 caaatattcc aaacttccga ataaattaag aaacactatt tgtatgtttt tttccttttt
     1141 tctgtagctt tatcaaaagt actcttttca taaagtatac gctatatttc tttgaatgta
     1201 tgtttttaca caattttttc ataatattca taaaaaattc gaatagctgc tactaatttt
     1261 ttgtttttgt tgcttgtcct gtctcataat tgatcgtgta gccatcttga acattgtaga
     1321 taaagacatt gaattcaatt tttttgtctt caatgctttc tgcttccaat tgcactcccc
     1381 tggctaccag ttcattcccc tcaaaatgag gagtgacacg gtagcgtaca tgatgatttg
     1441 tttctttgat atatgccgca atatcatttt cgtgagcaag catgtatgga gcattcaacg
     1501 aacgtgtccc tgtcatcaag tttttaatgt tgttattttg accagtcagc tggaaaccaa
     1561 ttaaatggct gcgattatat aaccattgcc cgtcacttaa tttcttttgt ttccaacctg
     1621 tcgggtcaat atacaaaggc tctctctttt cagtagggaa caaatctttt ccaagcatag
     1681 cattggcttg tcctactctg ttcaatttat caatatttga atagctttcc catgttcctt
     1741 tggaaaggct aagatcctct tttgtaaatt ccggctgatt cccattgatt tctatttctt
     1801 gatgttttcc atcatattct ggcacatccg ctacttgaga agcttgtggt gtggaatcaa
     1861 aaatagttgt aatctgctga aagatatctg aatctttttg tgtggtggaa acaccgaatg
     1921 cccctgcaat cacagcagcg acgatcccca cgatcgcctt ggttgttttt ttgctcattt
     1981 ctttactcct ttactaacag ctccctaaat cttaacatat gttcccctat aaataaatac
     2041 tagaaagaaa tagaaacagc aaaaaataaa acctgtttta agatggctta aaaatacaaa
     2101 aaagagacgg attcctcgtc tctaatcagt caccactcta tctgtaaggt atttattatt
     2161 attcaaaaat tttcttcata ctcaagacaa tacactgatt tcacaaccgc ttggtaaacg
     2221 gtgcgcaaaa gcttgcatct gcggtaataa gtccatccaa gttaaaaaca taagaagttg
     2281 tttttgcttg gctgttctta ttacttgttg cagagcagct ttttcacaac ctattttaag
     2341 cgggtgacgg gaatcgaacc cgcgacatca gcttgggaag ctgggatttt accactaaac
     2401 tacacccgca atacaaaaac agtatacgcc tcttttattc cttcctcaag tttttttatt
     2461 ctttacgctt ctggtatgtt ttgtagtaaa ataaaactaa tcaatcaatt attggagaag
     2521 atgctatgac gggaaaagaa ttagatctgt tgctggacac ttgtttactc gctgggaaaa
     2581 tcatgatgga aagcaatgcg gagatgtatc gggtagaaga tacgatgagt cgaattgctt
     2641 tagcttctgg aaatttccgt ttggtcagct atgtcactca aaccggtctc tttattggtc
     2701 ttgatcgtac ctcgaccatc cgaatggaac aaattaccaa ccgttcgatt aatttagaaa
     2761 aagtagtaaa agtaaatgat ctttcacgta aatatgttgc ttcagagcta acattagaag
     2821 aactttactg cgaactgcaa gcaatcgacc gagaccgttc tttctttcct gtctggttgc
     2881 aaatcgttag cgcggcagtg atcagtggaa caattatggt gttatttggc ggaaagatga
     2941 ccgatttacc tgtgacttta gtcattggag gattgggata cgcagcttat ctatatagtt
     3001 tgaaattttt tagaatcaaa tttttatcgg aatttttatc ttctttattg attggcatag
     3061 ccgctatcta cagcgtacgt tttggcttag gaagcaatca ggatctaatc attatcggat
     3121 gtgtgatgcc tttagttcct ggagttcaaa ttacgaacgc tatccgtgat ttgctagctg
     3181 gacattatgt ctcaggagtt tctcgcggaa cagaagcgat gatgactgct acgatgatcg
     3241 gttttgcgat tgcttttatt ttccagcttt tttattagga aaggaacaca agtatgggga
     3301 cgatatttat tcagttttct tttagttttt tagcgactgc ggcatttgcc gttattacaa
     3361 atgtacctag gcgttcattg atttgttgtg gtctttctgg tgcttttggc tggatgatct
     3421 actggatctg tgttcaaata ggcggatctg ctgcttttgg ttcattgctt ggttctttag
     3481 gagtagcatt catcagcgat cttttctcta aacgtttgaa aatgcccgta acgattttca
     3541 atattcccgg gatggttcct ctagtccctg gtggtttagc ttatcaagcg gttcgaaatc
     3601 tcgttactga atcctatcga gaagcggctc attatgcggt ccaagcaatt atgattgctg
     3661 gagcaatcgc tttaggtctt gtattatcag aagtctttaa ccataacatc cgtaatttca
     3721 aagtaaagcg tgacgcaatc atgatgaaac gaaaaaaacg gctttttaaa taaacgaagt
     3781 gtaacaaaga aaaggttgtg aaagagacgt tcagccttga gcgatgacaa aatatctcac
     3841 aaacattgcg acaagtaacc gcaggagcaa taagaagtaa ttatgagtat taaaaaaagt
     3901 tttgtaaaaa tagcttctca catttttaca taaaattgac ttaatactga gaaactgact
     3961 tttgaacacc ctctatctgt aaaaaagggg ctgcgacatg atttttgtca cggcccctct
     4021 tctttattca aattttttcc aaaacgttcg ttgataataa acgatccata gagcggaaag
     4081 tattagaact aaaccgatca caggaatatt cactaaccaa aatggataaa acaaagccaa
     4141 cattccgtat aagacgataa gaataatact gatgatcaat gaacccatca tagttgcgac
     4201 taaacccaaa cttcctgaac ctctgttaaa taattggtta atattggtcc agttcagcag
     4261 cagcaaccga taatctctag cgaaataacg tagtgacaat aagtagcagc cgaataaaga
     4321 accaaatagc aagctaggga taaaaaacaa aggcagctga aaagacagtc cgcttaataa
     4381 tgcgatccca ccagttaaaa aactctgcaa tatccatccg aatctgaact tttcttttaa
     4441 atattggttc atagagattg gcaaagaacg gataaacaga aaattttctt gatccaaaga
     4501 aataagattg ctgataaaag aagtctgatt aactgttaag aaagccaatg cgatccctac
     4561 caggaaaaca acaccgatta acttattatc taaatcagtt aattttattt cgcctcctag
     4621 ggcaaaagca agaataaaaa tgattggagt taacaaggaa gaagaaagaa cttgcataat
     4681 caaattaggt tcttttacta attgagcatt gtaactaaat aaaagctgat gcacattttg
     4741 gttcttttta tgggttcttt ttattttgaa ctcactgttt gaatcagtca gttgctctgt
     4801 aagctttggt actatcagct gttttacagc aaggaaaaat cccagagccg tgatagctag
     4861 tattccccaa cccaataaac cttgttgaga aagtggctga ctggcagcat catagaatgg
     4921 caataatatc gaaatcggat gccgatctgt taactcattt tcaatagtcc ccgtttgatg
     4981 attcatccac atgattccag caaatgcgat cagcatcgag ctgccaagca agatactaga
     5041 aactgttttt ctatgttttt taaacactgc tgttcgagcc aaaccaaaaa caatcaaact
     5101 agccagacaa aaaagaataa tcaaaaatag agaaaacaaa aggattgaga taatgatagt
     5161 aagcaaaatc cctagatcag ctcgccatcc ggttaaaata aacatgacta gaagcggaaa
     5221 aacaaaagga atgatggtca aagcaactac caaaatcttt gctgaaaaaa cctgttcttg
     5281 tccaaaaggc agaggcaaaa atacttgcag gtcatttcct tcaaaaaaga tattataaat
     5341 gactgatata ctttgagtta aacctaacat accaaaaagt gccacgtaat aagtaaagaa
     5401 acctggcaat ttaacaaagt tcatagcaaa catagcaaat ccgtaaatca gtaaaaaaat
     5461 cagccctgaa aaaagatttt gagacaaaag atagatagtt aatcgctttc ccttttttcc
     5521 tttctctctt gctttattcg tgagctgtgg attaacatat aaaagattaa ctcgaattaa
     5581 ttctaataat tgtcgcttat acatacggtc catctcctgt cgcttgcctt ccagccattt
     5641 tcaaataaat cttttctagc gtttcatcag gtgagccatt caataattca ctaactgccc
     5701 cctgataaat caattcacct ttcttcaaaa tagccagttt atcgcataac tgttgtgcgg
     5761 tatccaatac atgtgtagaa aaaatgactg tttttccttt tgcggcatga gccttcatca
     5821 tttctttcaa atcaaatgct gcttgaggat caagtccttg caaaggttca tccaataccc
     5881 aaatatctgg atctggcagc aacgtgccga tcaaaatcgt tttttgacgc attccatgtg
     5941 aaaaacttgc gattgtttca ttttgatgac tatacatatc aaaaagcgct gtcaattctg
     6001 ctaaacgctt ttcttttttt tgtgtgtcta attcataggc agctgaaatc aagtcccaat
     6061 attctccagc cgtcaattgt aaaaaaatat ctggggaatc aggcacataa ccgatttttt
     6121 gtttgatact caatcgattt tccgtcaatt tcattccatc tactaaaatc gttcccgatg
     6181 atggttcaat aatactaact aaacttttaa tcgtagttga ttttccggca ccattatgtc
     6241 ctaaaaatcc aaaaatttcc ccttgtctta tcgagagaga caagtctttt aatgcttgtt
     6301 ttttcccgta acttttactt acattgatga attcaatcat ttctgtactc cttttgtttg
     6361 ttattacttt ttattgtacc cttcgttatt ggtggagtaa atgtcaaaaa gagtgcataa
     6421 caaaaaagga tagaaacgac taggctgcta cttgggttgt tctcttctac aggaaaacac
     6481 tcttctaaca ggtaagatat gagtctttgt tttctttaaa atgattcatt ttctatcttt
     6541 tcttgcataa aattctttag tttatcaata cttctctttc ccatctctag ttgttctcca
     6601 tttgcaaatg ttactatttc gtttactctg ctaatagatg taatgttttc agtattaatg
     6661 atataagctt ttaaatcttt acataaatac tcatattcga gtgatttttt atatttttta
     6721 attgtgccgt ttacgagaat ctcttcatct ttcaatacta ggcctatttt atttttataa
     6781 cctggcatgc tcattagata aaggatttca tcgtaatgga tatagtgatc gctattacca
     6841 taacggacag ttgtccacct ttttttcgta gcatgttgaa caggatagtt ttctttttct
     6901 ttcaataagt aactttggat gcttttttta tttttttcat aagtgaattc ttttatcaaa
     6961 taaccactag gcgtaatctt tcgattaata acagttattg ctttattcgg atgagacgtt
     7021 agataaacaa ttttacactt ttcattcttt tctctaagca tttcgcctag ttcgataccg
     7081 ctataatagg cgttcaaatc gatgtctagg aaaaaaatat ctgaggaata gatttcatta
     7141 tttttaatga attcaacaaa ttgtataacg ttcaatccct gaacgatctc aaatttccaa
     7201 ggacttaata aaattctgga ttcttccaaa atttctacta gcattttttc aaaaaccaaa
     7261 ctatcatcca aaataaatat acgcgttacc ataaaaagta cctccactcg atattttact
     7321 atcaaaatca tttcatacaa cttttcaaca tacatgttag gaataaattg taattctctt
     7381 cacaatcttt tccaacacct ttgaaaccga tatcaaatac tttcaggatg ctgcttctaa
     7441 aataaatatc aaacaaatga atacatcatg caaaaaaagc cagacaaccg acgtccttgc
     7501 gtgtcagctg tctgagctat gtttatttat tttgttcttt taaaaagaac gatacacata
     7561 ctgatttttc ggagcagaga ctttctcagc gtttgtaaca actacagatg ggatttcacg
     7621 cttgaatggt tgataatagt ctgattggat ggttccttct attttatacc attcatcatt
     7681 tttaaccgtt actccttccg gcatatgcgt caacaaacca aagactcctg aatctgccac
     7741 acaatggata atgccgaatc ggaacaagaa gatatctgta ttgctatcgt tcggtgtttg
     7801 gtaaacaaat ccttcatagc tgattttttt gccaatgaat tcgctagggt aattataaat
     7861 tagctccata acttctaaat agttttcatc tgtgatcttg atagtttgct tatccccata
     7921 cttttctttc aactcattca tttggtctaa gtagtctgat ttgttgaaat aaatactggt
     7981 atccggcttt aagtattgtg tctgcatctc cggatcccct actgattctt tacttactgg
     8041 gaaattaaat cctttggctt ctacgatcgt agtgtcgaga ctaactgttg gaaacaacgt
     8101 ccccacgatt actggcaaag ctagcagtac ataagcgatt ccacgttgat aaggttttga
     8161 caacccatgg tcatgatcgt gctcgtgtgc cttttcttct gcttttccgt ttttgaccca
     8221 caaatacagc tgaactaaag ccaaaagagc agataaaatc attgaaagaa tagctaagta
     8281 gcgataatgg acattgatat attgatcgag tttgccagaa acttgcaagt acatcatcaa
     8341 ttcgaaatac ccagataaaa tcaaaaaccg cagcatattt tccctcctaa ataaataatg
     8401 tgaatccaat aacgctaagg gcaatcagtc cgatcagcga taaaataaag cgtcctgtaa
     8461 aatatcgttt catcatcagt agatttttga tatcgaccat tgggccaaag accaaaaagg
     8521 cagctacagg tgctgtgccg aacagactta acaaagaaga tcctataaac gcatctgctt
     8581 ctgagcaaag agacatcgta aaggccaata acagcatgat gataatggct aaaatctttg
     8641 ttgagccgag cgttagcata attctagttg gtaaataagt ctgcatcgca gctgcgatta
     8701 atccgccaat gatcaaataa cgacctgtat caaaaaattc gtcgattgaa tgtgtcaaca
     8761 catgcccagt ttgatggatc agctgatttt ttggttcatt cttctcactt tgttcttctt
     8821 catggacatg atgatggtga ttttctgcta ctttagacgt gattttttct ttcaatacag
     8881 cgtctttttg aaaataggca agccagacac caatcacaag ggcaacaaaa aagctgccta
     8941 tcacccgcca gcttgccatc tgccaagaat tgccaaatgc gataaaagtg gaaaaaatca
     9001 cgattggatt gatgatcgga gcagtcagca taaaagcaaa cgctgtgtga actggtacgc
     9061 cttttttaac aaattgatga acgatcggta cgatgccgca ttcacatgaa ggaaagaaaa
     9121 atcctaagat cgaaccagta aaaatagaga agatacgatt ttcaggaagc catctcttca
     9181 cacgttctgg ggtcaaaaat acttgtaagg cgcctgagat aagacagcct aataatacga
     9241 aaggtaatgc ttcaataacg atcgataaga aaatcgtact catctgcaaa accgaatttg
     9301 gaataaaaga aaacatgagt ttcctccttt ttatttctag ttcattcgct ttgcaaatat
     9361 acgcttttat tcaaaaaaaa caagttgctc tgtaaatttt tacacaactc ttatttttta
     9421 ccaaagcact tcaattgact ttttctgtta ttcccattaa attaaggtct catacatatc
     9481 acttctatca taaaagggag aataaaaatg aaacaaacca ctaacttgac tgaagttcaa
     9541 gacatactgc aacagtcatc cgttgccgtc atttatcttt cgatgcctaa ttgcagtgtt
     9601 tgtcacgctg ttcgtccacg actagaggaa cttctcacac attatgatat tccggcactt
     9661 catttggatg catttgaaac accagaagta gctagtaaat ttgaagtgct gactgcgcca
     9721 gttgtcctga tttttcatca aggaaaagaa gtctttcggc aagcacggtt catcgacttt
     9781 aaaaaaatcc gttatctact agatgaactg acagcagaag atgattccct ttcttatgaa
     9841 gaaatcttcc gaacaaaata atcgttgtcc aaacagaaaa aagcgattga acaagtgaaa
     9901 gttttgcctt catttgttca atcgcttttt atttttattc tgctgtattt tcggaatgcg
     9961 catttgcgtc agaatgcaag aaatagatca aggtttgcaa ttcattcgtc aaatcaacat
    10021 tttggatcaa tacatctgac ggagcattca atcgagcagc ggtaaagttc aagattcctt
    10081 tgattcctgc ttccgttaac tgattgacta cttcttgtgc tctttcagcg ggtatcgtta
    10141 aaattgctac ttcgatttgt tggatcttga tttgttctga catatcttcc attggataaa
    10201 cggggatacc gtctacgatg cgcccaacga tttcctcatt cacatcaaaa gcacaactga
    10261 tacgtatgct attactttga tggaatttgt attttagtaa agcacttccc aagttcccta
    10321 caccgatcag cgcaacattt gtcaattgat cgtcgtttaa tgtctttgca aaaaagtgca
    10381 taagatcttc tacatcataa ccatacccac gttttcctaa ttcgccaaaa taagaaaaat
    10441 ctcgacggat cgttgcacta tctacttgaa cggcttcact taattcagtt gaagacactt
    10501 ttttctttcc agcattattt aaaatcctta aatagcgata atacaaaggc agtcttttag
    10561 ctgtcgcctt tggaataata tgatctttca cgaaatgacc tccacgtatt gtctaattac
    10621 atctttgatt tctccatcat agcagtattt ataggaaaaa atcaattcca ttgctcagat
    10681 attgtgaaat tttattcttt attcatttat ttatcatttc tgcaaatctc gcagtataat
    10741 ggaatttatg gtagactact aattgaaaaa tagtttttta tttgaggaga attcacaaag
    10801 tagaaaagag gattccatga tattattaca agccaatcag attgctcgcc attttggtgc
    10861 agaagtctta tttgaaaaca tacatttaga aatcagtaca ggcgctcgtg tcgctttagt
    10921 tggcagaaat ggcgctggaa aatcgacttt attaaagatc attgctggaa ttgaagcacc
    10981 tgatgaagga aagatcgcta aaaataaaac agcgactctc ggatacctcg ctcaggacac
    11041 aggacttgat tccaatgaaa cagtttggaa cgagatgctg aaagcttttg aacaagttag
    11101 acaaatggaa gagcggatgc gcgagctaga gattttcatt agcgaaggta cgccagacac
    11161 acctgcttat agttcttatt taaaagaata cgaccggctg caacacgatt tttcagatcg
    11221 aaacggctat ggttatgaaa atgaaattcg ttccgtattg catgggttta aattcgattc
    11281 ttcattttat gatcagaaaa tcgatacttt atcaggcgga cagcgcacac gtttagcttt
    11341 agctcgcatg cttttgcaac accctgacat tttgattcta gatgaaccaa cgaaccattt
    11401 agatatcgag acactttcct ggcttgagaa ttatctgcaa agctatactg gtgcattatt
    11461 gattgtttcc catgaccgtt attttttaga taaagtagtc aatgaagtct atgaattaag
    11521 cagaagaaaa atgcgccatt acaaaggtaa ctattcgaaa tatctagatt tgaaagcaga
    11581 acagttagca agtgaatgga aggcttatga aaagcagcaa acagaaatcc ataaattaga
    11641 agacttcgtt gctcggaacc ttgttcgcgc ctctaccaca aaacgagcgc aaagccgcag
    11701 aaagacatta gaaaaaatgg accggctgga tcgtcctcaa ggggatgaaa aacaagcaaa
    11761 ctttctattc agtatagaaa agccttctgg aaatgtagtc ttgcaaatcg aagatgccgc
    11821 tatcggatat gataatgtca ccttatctga accaatcaat ctagatatcc gtcgtcaaga
    11881 agcgattgct ctagtaggtc cgaatggtat cgggaaatcg acgttgctga aatcaatcat
    11941 tcaccagatc ccttttatcc gtggaaaaga aacgctaggg acaaatgtca ccatcggcta
    12001 ttacgatcaa ggtcaagctg atctgcatac aaacaagacg atcctagcag aattgtggga
    12061 tgagcatcct accactccag aaaaagagat ccgaagtgct ttaggtgctt tcttattttc
    12121 tggcgaagat gtagaaaaac caatccctct gctaagtggc ggtgaaaaag cacgggttgc
    12181 tttagccaag ttagcgatga acaaagaaaa ttttttgatt ttggatgaac caacaaacca
    12241 tttagatatt gacaacaaag aagtattaga aaatgcattg atcgattatg acggcacact
    12301 gctttttgtt tcccatgacc gatatttcat caatcggatc gcgacaaaag tcgtcgaact
    12361 ctctgaaaat ggcagcaaag tctatctagg agactatgat tattatttag agaaaaagca
    12421 ggaagaagaa gaaatcgccg aattgttggc acaagatgag ccagaatctg ctcctgctcc
    12481 cacgaacaaa tctgattatt accaaagcaa agaacagcaa aagcttatcc gtagccttca
    12541 acgaaaaatt actcaggttg aagaagaaat ggcgcggatc gatgaatgga tcgatcagtt
    12601 ggaacaagaa atggtcgcgc cagaaaacct ggaagatcac gtaaaactca atgagctgaa
    12661 ccaagattta gaagctgcta gacaagatca agaaacaaaa ttgacggaat gggaagaact
    12721 aagcttggaa ttagaagaat atgaaaatta acgaaaaaac caaaacagaa accattcatg
    12781 ctgttttggt ttttcttttt ttattttatc aaacaaaaat tccatattta cctttttcac
    12841 caataagaag accaattact agtcattctc actaaagctt ctaaataata gtaatcaccc
    12901 caaatacaag gtgtatttac cccattattt ttgggcttat tataaacaga ctgtgttaaa
    12961 aagccatctt cttcttccgt tgtagcatat ctttctttca attgtcgcat aattttccaa
    13021 acagcagact ggtataagtt cttttctgtt ttgtctaata gacctgattc tattaattct
    13081 aataatccac aaaccatgat caccatacta gatgtatctt tttcttctct gctagaatca
    13141 tcaaagatca aatcccaata acaaatggta tcttctggca accgattcat aaaataattg
    13201 gctaatattt tgcttaaatc aaaatatttt tgttctttca aataacgata acttaatgca
    13261 aaaccgtaaa tcccccatgc ttggcctcta gcccaacagg aattatcact ataaccttga
    13321 tgcgtacttc catatcttgg ttttccatct ttgacatcca tataaaatgt atgataagtt
    13381 gaaccatctt ctcttactaa ataatctgct gcattttgaa tatgagcctg agcagctttt
    13441 ttatattttt gatttccagt ttcttcactc gcccaataca ataatggaag attcatgcaa
    13501 caatccatga tcattctacc ttgttgtgaa ggatcactag gatctcccca agcttgtata
    13561 actcctgcct tctcccaaaa acgctgaagt aatgtttctg cagcatcaat tgaagcttcc
    13621 ttcatttttc gatttcccgt tattttataa tctgctacgg ttgataaaga atacaaaaaa
    13681 ccaagatcgt gatgattagt gataatttga cagtcaatcc tgtggataaa atctatatct
    13741 gctttttcag ctgtttgttg aaataaagga tcacccgtct tctcaaataa caaccaatta
    13801 attcctgtcc aaaatccttc tgtccaacca atattgtctc ttaatggata acgtcctttt
    13861 ataggacact cgcttgggaa ttgatactga ctgttactga tacgtttgat taacctctcc
    13921 gtgtgtctgc gacaacgctc gatctcatta ttccattctt tttccattaa ttctttataa
    13981 cattcattta tccatttttc tggatgtatt atctcttcaa acatcacttt atcaagcatt
    14041 tccccaccag cctttagcat aattttagtc tttttttatt gtttttattt tggatagtta
    14101 gcccggttgt cccatcaaaa tcaattttgg gacgattgtt ccatacttct aaactataat
    14161 cccgatcttg atgagcaacc actcctgttc cgagccaatg ggttcctttt ccaaatgtac
    14221 cctctaatcc aggaataaaa gtgttttccc atgtcatcaa gtttgtattg ggcaaaccca
    14281 taatagctgt ggattttcgt ttaatagtat cgtcacttaa acagataata cctgaaaagc
    14341 cagcttcact caccctatag ttttccttgt cttcttctat gattattcct tctttctcag
    14401 gagaacattc aatagcaaac cctgtctcat atagaacata ttctgtatct aattctactt
    14461 tgtgtacgcg aatctgccag ccttcaaaag gaataagcca agttctgatt tttgcctgta
    14521 aagtaggttc ccatgtagag gcaagtacct ctttcttaca tacaatgtct cgtacattat
    14581 ttcgaacgat gaactgtcca taggttttga tgctctcatt cttaaaactg gtcgtgatat
    14641 atgtgtccgc tcgctgaatc cctagcattg aatccattgc catttctgct ttcaattgat
    14701 tcgctcgcgg tatcgaaaaa ccaaattgac tagaataagc aaattttaaa tatttttcat
    14761 tggtcaatgt atggaaattt ggccctaatt gtcccgcatt caaaaaaaat gtatgtccat
    14821 tatcatgttg tatgagcaaa ttggcttctg aaagaagttt agttccttca cgaataggca
    14881 taggcatttc tttagccgtc caatactcat ggtcttcagg tagcgccaac aataaaaaaa
    14941 tcttattcag ccataatgga gataacgtac tattataggg ttcagtcaat gtcaattgag
    15001 ggtaggtata accaatattc aggatacctt tttcatcaaa tatttctttt tgcatccacc
    15061 atcttatatt ccgattgact aaccccttga tttcacctaa tttaaaaggc aaaagatcgg
    15121 cccacaacat agctgaccaa aagcttgaaa ctgcaaaacg ataaatcatg cttcgaccaa
    15181 aaggcacatt tgcaccatct tccgcaaaaa aatgaatata gtctctagaa aaatcaatag
    15241 ctcggttctt atatatctgg ctaatgggct tttctggttc tagtacgcta taaatcaaac
    15301 cataataatg aaatgcaaaa ggattataat aatcttggcg acctcttgaa ctgtctcgat
    15361 accagccatt ttgaagataa caattttcta tattatcaaa tgcttctttc agtgctgatt
    15421 ctttaactgg ataaccaaat ttttggataa ttgttccgac aattatctta aaaaattgcc
    15481 aattaccatc tgcacattca tattcaaaaa tttgcgagaa ccaatgaata atctggcttt
    15541 tttctcctgt atctaatttt ttccataata attgttcatg atgatataac gctaataaca
    15601 ttgcgggcat ttctaccata cgctggtctc tttcatgtat ttctccccaa taatcgggat
    15661 gggaaggatc tgttccagct tttattcctg atagaattaa tttcaaccat cgttcatcta
    15721 attggtcaat tgcgggcccc gctccccata aaacccgaga gaatccttcc attcctgcaa
    15781 ttttatttcc atatcctacc ccttgatgta tataatcaat ttgtgcgcca cttgctcgat
    15841 aaaacggctg gagtggttga tttatctttt taaataagtc aaagtaatcc tgtctggtcc
    15901 ttaatagtgg tactggatgt ttcattgtat tttctcctct ttttactacc aaatagctaa
    15961 ttccgactgt tctaatcgaa gtaagccttc aataaaaaaa taatcagaat agatcagtgg
    16021 cacattataa gtcagttctc tatggtatgc ttctgctccg cctccaatga tcccatcttc
    16081 atccatattc caatttgcat agtgcttttc tgtcttttgg agtatattga tagcagcttc
    16141 atgatacatt tttgatcgta cagaggaaac atatcctgct aattccagaa gaccgcatgc
    16201 agcgcatact ccagcactgg tatcaatatt cgaatctttt acagcaaaat caatgagagg
    16261 aatataacca gttttttcga ttttctgaat aaaatttact gctatctttt ctgctatagc
    16321 tagatagtac ggattttttg tgtaacgata acttaacgcg aatccatata aagcccatgc
    16381 atttcctctg ctccaacaag aattctcatt gtatccttgc ccgccaagta accgaatata
    16441 tttccctgat tctgcatcaa attctgcgat atgcccggtt gatccatctt tcctcactag
    16501 atacttagct gttgtatctg catgctgccg agcaatttta gagaacctcg gatcaccagt
    16561 ttccctcgaa gcccaataca gtaatggtaa attcatcata ctatcaataa tcatccaacc
    16621 aggtttatgg tcattccatg ctactaaata gttccctgaa atattatagc gtccagcaag
    16681 taccgtagct gcatgtaacc ctagttgtcg actatgtttg ttttgagact gttgataatg
    16741 tgcaatagcc gatggtaaga ataaaaaacc tacatcatga tgaacgtctg aaaaatggta
    16801 cagggcagat tctaataatt gttcttgttg tattgctgcc tctttgaact tttctcgttt
    16861 tgtcttgtga aagagctgcc ataaaatacc actccaaaaa ccattggtcc accagtcgat
    16921 tcctctgtcc tttattacgt ctgtatattt tccgttgtaa gaaatatatg gaattttatt
    16981 ccccattctg tctaattccg catctatctt ttcagaagct acattcaaaa tttttcttga
    17041 ccattccttc gtctgttcat tcatggatat cccgccattc ttttaatttt tatgaatgtg
    17101 gagttgctcc tagaacgaac tagatggtta acattcgtta ttttttttac ttatttgcag
    17161 ctccaatgtc attctcctta aatttagact gcgcccacac ataaatcaag accattggga
    17221 tcactgaagt tgctaaaatt agcataattg cgccataatt aattggctca cctgtactag
    17281 aaaccgtcat atttttgata attaagggaa ccgtatattt atctttatct gaaagcatga
    17341 ctaatggaaa tagatattga ttccaacttc ccataaagat aagtaaacca ctagtaataa
    17401 tcgtaggtga taaaatcggt acagcgattt tcgtaaaaat cacccactca ttggcaccat
    17461 cgattctggc agcctccatc agtgctgtag gaaattgact gctatattgt cgcatcataa
    17521 acactgaagt tgtactggca atcgaaggga tgatcacacc tgcataagta ttcgataatc
    17581 ctaaacgact cataatgtaa aataaaggaa tcaatgtggt aaaaaaaggc accatcctag
    17641 atagcataac tgtattgaac aacagtttat tccccataaa acgatacttt gctaatgcat
    17701 aacccgccat tgtagaactg gctgtagcta aaactgtact gattacagca ataacaatcg
    17761 aattccaaat aatcaaggga taattatatc tcgccatgat ggcttgccag ttttttaata
    17821 gcatatcccc aaacttgaaa ttgattccgc cacttaaaat ttcttgatta ctttgcgttg
    17881 aagctacaat catatataaa tatggcatca aaaatattaa ggcaccaaac acaataatga
    17941 cgtacgtaaa aattctgctt tgtatctttt gtttagtcat ttttatcacc tccgaattta
    18001 aattgaatag ccgataatat aacagataca aacaccatcg tccaggcaat agcagaggct
    18061 tttccaaaat caaccatttc aaaacttgtt ttatataaat tgactcctac gaccaaagct
    18121 gaattttctg gtccattgga agtcccaaag aatatatttg ggatattgaa attacccaag
    18181 ccattgatag tggctaaaac tgtggccatt aaaatgattg gctttagcat ggggatcgtt
    18241 aataaaaaga attgccgaaa tttcgatgct ccatctattt cagcactttc gtatatctct
    18301 ttagaaatcc cttgaagacc agctaaaaat aaaatagtgt tatatcctac actaagccaa
    18361 atcatcgtga tgatcattga tattcgtatc caaaaaggat ttgttaacca ctgtacaccg
    18421 ttaattccta gcgcattcaa aaatccattg aataacccag tcggattgaa aaaagtcatg
    18481 aatacggaag ctacagccac cgttgatgta atggttggaa tataatagac agttctaaaa
    18541 aatccttttc cttttaactg tgctgaattc aataaaacag caaagtaaat agctaatagc
    18601 atcgttattg gaattgatcc tgccatgata atcaaaacat tcgtgtaaga tttaataaac
    18661 aatggatctc ggaagatagc tagataattg tcaaatccaa caaattcaaa tgagtagcca
    18721 attttcataa aactaaaagc aaacgagata atgatgggaa tcaaaatcgt cacaataaag
    18781 aaaataacaa aaggtgtgac caatccataa gcactttttt tatgagacgt atctgttgct
    18841 tttttcttgc tgttctttat tgtcttgctt ggtaacgaag attgcatcat cgttaagtcc
    18901 tcctaatatt ttttaatcac cagttaattt tacgccgtac ttatgagcaa aattttctgc
    18961 ttcttttaga tatgaatttt caaaatcacc tgtttgccaa tagttatatg cagcggtagt
    19021 caaataagac gtagcatctg aataagtggc tggtaaaaca gtatttgatg tattttcatt
    19081 gattttattc aatagttcat agtatttttg tcctccttag tagtcaaact ttttctcagc
    19141 tgcttctgtt tgataggcca atgagttaga tgtagccacg cccagctcca gtgcgttggc
    19201 taagcattct tcatctgtca gcataaatgt aagaaattgt tgtgccactt gtggattttt
    19261 ggcatcttta gggacataaa aacttgatcc tcctgatact gcaccttttc ctggattttc
    19321 ttttgaaaaa ggaatcagtg gagccatgcg ccatttgtca gatgcatcag gaaaattcaa
    19381 agacatattc gtagcatacc agccgccagc aacaaacatt gcagattctt gaaatgcttt
    19441 ctcagcatct tgtgcaccgt agaaatgtac aatatctgag tcgatcattt ctttgattaa
    19501 ctttgcagca tgtttcgctt ctttagtatt cagacggata tttccttctt catcaagcaa
    19561 agattgattc tgctgggcta gcatgttgat aaacaattct tggtccccag attctggcaa
    19621 ggcaattcca aaactaccag ttttttcttt taatgctttt gccacctgga tatattcttc
    19681 ccaagaactg atttcttcaa aatctacacc tatttcagca aatttttttg gttgataata
    19741 acaaagtgct ggggtaaaat cactagccca aggaataatc gtttcattac tcgtttgttg
    19801 acgcaaaata ttcaattttt gatcataaaa ctgatcgcca tatttgtcat aaaatccata
    19861 ttcatcagca ctataaaaag catcttgaaa tttttccagc acacctgctg ctgaagtgtc
    19921 tgccatggag ataatatccg gcatatcttc gtgtgttacc aattttgggg tgatcatttg
    19981 tactgttgct gttcccgaag ctacattcac aatttttaag tcaatatttg ctccagtttt
    20041 gcggttatag atttttgcag cattttcaac atagtttgtc agtttgccgt ttgcgacata
    20101 tacttctaat gtagtggctt tttttatcat tggatctgcg atgtttgctg ttacttcttc
    20161 gttagttttt ccacatgcac ttaagctgaa taatagggta atgtttagca aagctatcac
    20221 caatttgtat ctttttttca tgtctatccc ttctttcttt tcaagttctt atataaaacg
    20281 ctttcactta tgtatattaa cggaattagt aacacaaata tttatataaa aaaatatttt
    20341 acttgtcgtt ttggcttttc atttttgtta taatagtcat taattataaa tagaaagctt
    20401 tttatataaa aagagaaagg aaagtggttc ttttgttaac aaacaaccaa tcctccaatc
    20461 ttgtcaaaaa aacactaacc tattccatag atacccatct tcatttatct gctgctccct
    20521 attttcgact actaataaat gaaagcgcta atttatatgc tgaatttgca cataaatcta
    20581 tagaactaga taatccttcc cttttattca ttgataaaca gcaagaagtc caaatctttt
    20641 ctactcaaaa ggaaacacaa acttttatca ttatccagtt ttattttgat gagcaattat
    20701 tagcacatat tacgggacaa tctttttcta tagatcaaga aataacatct atcattactt
    20761 ttctgaaaaa tgaacagttc aaaattgatt atctaaaaca tagtaagcca gctatttcct
    20821 atgatcagga aatcaattct tttttcatac tatgccaaca gatgagcatt catttgcttt
    20881 ccttattagt tacttattgt ctgcaaaaaa ttcaattgtc ttccttacca aggcattatc
    20941 aacaggaact tgctttattg acaatacctt caaatgagaa agtcattcct aaaaaaaagc
    21001 atattcttta ttcagatgaa ttagttgatc caatcatacg atatttagaa gaacatcttt
    21061 cagaaaattt tttgcttgaa caattagccc aaattttttt tatcagtcca gccagcttaa
    21121 aaaaacattt tagaaaagtg aatggaaaaa gtgttattag ctattttaaa gaactaaaaa
    21181 tggaaaaagc aaaacgtctc gtgctagaaa atgaattaac ctatacagaa attgcagata
    21241 tattaggatt tcattcgatc catcattttt cttcagcttt taaaaaatat acagggttat
    21301 ccccttcaaa gtattatcta tccttaaaat agaaaaaaat ttttaggttt tatccacaaa
    21361 ttatttctct atattttctg tctattcaaa ataaacaata attttccggc acaattattt
    21421 gctgtttttt accataacat agtacgatca acgtagaaaa ggagatgttt ttatgaaagt
    21481 tgttgtcatt ggcggtgtag ctggcggtcc ttcatttgcc actcgtttcc gtcgattgaa
    21541 tgaagcgcac gaaatcatta tctatgaacg cggagaaaat atttcttatg caagttgtgc
    21601 tttgccttat tatttaggag gtgtgatcac tgaccgtgac tcgctgatcg aacgtacacc
    21661 agaaatattg aaaacaaaaa acaacatcga tgtctttact aaacacgaag taacagcaat
    21721 cgatccttct actaagcgat taacagttaa agacctatcc acaaatgaag aaataaaaac
    21781 agattacgat aagttgatca tctcttctgg tgctagacca gattatccgg atattcccgg
    21841 agtttttgaa gcagaaaacg gctttgtcct ccgtagtgtg acggatgcgg atcgaatcaa
    21901 atcgttcctt gaagaaaaaa atccacaaca tgtcgtcatt cttggtgcag gtgttatggg
    21961 tctggaatta gctgagaatt tcaagcatcg cggcttagac gtgactttaa tcgatcaatt
    22021 gccacaagtc gctttccctt atgatccaga aattgctaat ttagtttatg acaaattgct
    22081 gaaagaagga ttagccgttc atttagaaac aagagttact gagatccgtg ataaaggtcg
    22141 agaaatagta ttatcagatg gctccgtcct ttctgctgat atgctaattt ttgctgttgg
    22201 tgtttctccg aagaatgaag tggtgaaagc aaccggcata caattatcag atacaggtca
    22261 gatcattgtt gatgaccagt tacaaaccaa tctgccggac atctatgcga ttggcgatat
    22321 tatcgaaacg actagtgtag tgactggtca gccgatccaa agtatgcttt ccagtgcggc
    22381 caatcgtcaa ggacatatgc tggcagatat tttaaatggt acgcctatgc gctatcgcgg
    22441 atatattggt gcaggtgtcg caaaaatctt tgatcataca gcaagttatg cgggaatgac
    22501 agaacatgaa ttaaaagcat caggcataac aaattataaa actgttttta tcactccctt
    22561 tgaccatgcc tatttctatc cagaagctac aagattaaat ctaaagctga tttttgatgc
    22621 agatagcggt cgtattttag gtggacaagc atttggagaa aaaggtgtcg ataaacggat
    22681 gggagaactt tctgtagcaa tcaccggaaa cttgacagtc tttgatttgc ctgatttgga
    22741 gttgccttac tctccaccgt attctactac ccgtgacccg ttgaatatag ctggttatgt
    22801 cgcaatcaat caaatgacga atatcgtaga aacgatcaaa gcaagtgata tacccgaaaa
    22861 cgatttgaaa gaagcgttct ttttagatat acgtgaacct aataaagcac cttctggcag
    22921 tatctcggca acaaaaaata ttccaatgaa tgagttacga gatcgaatca atgaaatccc
    22981 aaaagataaa aaagtttaca ttactttcag aagaggattg aatacttata cttctgcccg
    23041 aatcttggca ggtttgggta tcaaagcgat tttgattgaa gaataaaaaa acgaccgcgg
    23101 tgggtagccg cggtctagga gttattttta cttgaggagt aaaaatgaaa aagtatatag
    23161 ggttgttggt tggtatgtat atagaataga agataaatgt gaagaaattg tgcattattt
    23221 cttcacattt attaggttat agtttaagaa agtttaaaca ttgcgcaatt taattatttt
    23281 agctgtttct tttcataaaa aaacatgttg tgactctgag acaaatcata gtccatagtg
    23341 atgtctctag agtaactacg cctaatatta cgaggcatca tattaatcta tcagcactag
    23401 tacctaaact agcatccctt tcgaatattt tttcttatat aataaagttt gctttcactc
    23461 ctctaatctc accaaatcga ctggcttgcg ttgtgtgata ctaggaactg attggtttag
    23521 aacgactttt ctattgtaat catatttttc gccatcctcg ttgtcttcta atccttcaat
    23581 gaatgatccc taatgtgtgt attaaataat ttaatttttt tgttcctatt tatttaa
//
LOCUS       DACDXE010000043        23432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.043, whole genome shotgun sequence.
ACCESSION   DACDXE010000043 DACDXE010000000
VERSION     DACDXE010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.043"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(10..2154)
                     /locus_tag="IWM23_001704"
     CDS             complement(10..2154)
                     /locus_tag="IWM23_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9884338.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQTADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFEPVPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(2511..3884)
                     /locus_tag="IWM23_001705"
     CDS             complement(2511..3884)
                     /locus_tag="IWM23_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884339.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPIMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWM23_001706"
     CDS             complement(3884..4279)
                     /gene="rbsD"
                     /locus_tag="IWM23_001706"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAP9884340.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGSEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWM23_001707"
     CDS             complement(4307..5224)
                     /gene="rbsK"
                     /locus_tag="IWM23_001707"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAP9884341.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(5333..6346)
                     /locus_tag="IWM23_001708"
     CDS             complement(5333..6346)
                     /locus_tag="IWM23_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884342.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQISHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEESGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(6427..7029)
                     /locus_tag="IWM23_001709"
     CDS             complement(6427..7029)
                     /locus_tag="IWM23_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAP9884343.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKGIVNNGTLSPHTAQSILTNN
                     DLLAKTLKRTAALKVLVNVDITTFSYLSHQTDAGGISALSAPAEFTDLLLKDSLKDTN
                     NMLTITKYNPSAFFGTSLDLQLRRRGIETIVLSGVATTNGVYATALDAFQHGYHVVLA
                     EDACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            7214..7915
                     /locus_tag="IWM23_001710"
     CDS             7214..7915
                     /locus_tag="IWM23_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884344.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            7925..10636
                     /locus_tag="IWM23_001711"
     CDS             7925..10636
                     /locus_tag="IWM23_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAP9884345.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEHNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKITEGKAQIAQGRTQ
                     LEDAKKQLAQQSAAIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKAAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWSYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            11033..12661
                     /gene="treC"
                     /locus_tag="IWM23_001712"
     CDS             11033..12661
                     /gene="treC"
                     /locus_tag="IWM23_001712"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAP9884346.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRITIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(12721..13902)
                     /locus_tag="IWM23_001713"
     CDS             complement(12721..13902)
                     /locus_tag="IWM23_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9884347.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSVW
                     LVPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            14154..15680
                     /locus_tag="IWM23_001714"
     CDS             14154..15680
                     /locus_tag="IWM23_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAP9884348.1"
                     /translation="MKRLIGILAMLVLAGMLTACGGASGKAEDSKEKLSVMTTFYPMY
                     DFTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQK
                     TLQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVAN
                     IRDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLAL
                     EYGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGV
                     SLEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNG
                     YFEDEDVQDRSLSDWAGTWQTVDTYVEDGTFAPVFEYKEKLNQDKTAQEYKEYYTKGY
                     QTDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGA
                     FRYIQFRDHTIAPADSEHFHLYAGNESQEALLKEMENWPTFYPDTMDGREIAQEMLLH
                     "
     gene            complement(15749..16477)
                     /locus_tag="IWM23_001715"
     CDS             complement(15749..16477)
                     /locus_tag="IWM23_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884349.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(16490..17770)
                     /locus_tag="IWM23_001716"
     CDS             complement(16490..17770)
                     /locus_tag="IWM23_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9884350.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIALSAFLSALLAFCFKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYAQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLVVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(17911..18513)
                     /locus_tag="IWM23_001717"
     CDS             complement(17911..18513)
                     /locus_tag="IWM23_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAP9884351.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            18759..18965
                     /locus_tag="IWM23_001718"
     CDS             18759..18965
                     /locus_tag="IWM23_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884352.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            19278..19997
                     /locus_tag="IWM23_001719"
     CDS             19278..19997
                     /locus_tag="IWM23_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9884353.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(20078..20761)
                     /locus_tag="IWM23_001720"
     CDS             complement(20078..20761)
                     /locus_tag="IWM23_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAP9884354.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            20993..23179
                     /locus_tag="IWM23_001721"
     CDS             20993..23179
                     /locus_tag="IWM23_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9884355.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         7812 a         4210 c         4583 g         6827 t
ORIGIN      
        1 taatttattc tatttcttta aaggcagatc aagccagaat gatttgtcta aacttaacaa
       61 ggttagtaca ggaacagttg cagctttaac tgtacgagta gttgtttctg gtatctgtct
      121 tgccgtaaaa ttgttccaca aatcccagat tgaagcgaca cggaagatgg aagaagcgtt
      181 gttgacgcgt tttatcgttg aaaattctaa tcctgtgatt ggcacgccat ctgttgtcca
      241 tttgattggc acatcgaatc gcttagacat attgaggatt tcagcgtttg cttcttcagt
      301 tgttgctgct tcttttttcg gttcatcata ttttgtcagg tcgtacgctt cgatgatcgc
      361 gttcaatttt tgtgaatatt ggcgatctgt agcataacgt ccttccaaat atttggttgc
      421 ttctttgtag tcttttgttt ccgacttcca agttggtttg tagaaatcag gattgccagt
      481 cagtccattt ttgatcaagt cggcatagtc ttccaaactt tccttgtaag aaggatattt
      541 acggaattgt gcgcggattt ggtagctttg tccggaacta tcttgttcaa gtgtattgaa
      601 ggtcacgctg cttcctttat atgatccttt gataccaaaa aggttatgat ttggttcgct
      661 tgataataag ctgtttccag atccactttc aaggatagct tgagccatca tgactgatgc
      721 gtagattcct tctttttgcc cgatttcctg tgcatcatct gcaatctttt tcaagaactg
      781 atcagttgtt tggttttgac tgaaatggaa cgagccatct aatccatcta aaatagatga
      841 gccgcctgtc agcgaacctg aagcagcagg taaacctgct aagtttactc ggacagcaaa
      901 atcaggcgtg aaatctgaaa tagaagttac tcgaacttta tctcctggtt gaggcgcatg
      961 gataaattgt ccgttcccaa tatagatacc tacgtggtgg gtaacaccag aagaaggtcc
     1021 ccagaagacg agatcaccag gttgggcctg acttactgga atctgtgtac ctgcatattg
     1081 ctgttcgcct gtccagccac cgatataatg accggttgct ttcatatata cgtaataagt
     1141 caaaccagaa cagtcaaaag tatttggacc ttttgctccc catacatatg gttttccaag
     1201 atgcttgaat gcttctcgaa cgattgcttg ttggacattc gttgtatttg taggcaatac
     1261 agcatcaatt ggtgatttta caacaaaatc agaagaagca gaagatggat tctctatacc
     1321 gctttgattt gctgtatttg gacgagatga agcattgttc gttccgccat ttgatgaacc
     1381 attgtttgga tggtttggtt ctctctgagt atttccattg ttattcgatc cagatgggtt
     1441 tgtttgattc gaagaattcc cgctgttcga attattgtta ttattgtttg aattatttga
     1501 accattatta ttgttgttat tacccacatc tggtttgttt ggcgtttccg gatctggtgt
     1561 actagggact tcttcactag gatcagttgg tttctctgtt gaagtatccg gtacaggttc
     1621 aaattctgca gtaatagata aattgttcgt acctactgta aaggtctttg catcataatt
     1681 tacttctttg gcaccattaa cagcgactga agcaatttgt tcaccttcag ctggcgtaat
     1741 agtgtaagtt accttcgttt ccgctaataa accttctacc gttccacttt ccgtaatatc
     1801 caaaggttgc tgttcagggg aatccgctgt cgaagcagtc agttggatct taccaatcaa
     1861 agaaggagca attgtaaggg aatatgtctc ggctggcttt tcattttctg atggatcagc
     1921 cggatcaatt tcagttggct cggtcgatcc tgttgttgag ttagttgttc cagttgggtc
     1981 ttctgtaaaa gaatccgttg tttcactagt agctgagctg gatgtagtcg tatctgttgt
     2041 gactgtttga tcagctgttt gattatctac ggcaaatacg gtattggtcg acaaaacaga
     2101 cggagcaatc agccaagtag cagctagcaa acgtatcgtt tttttatttt tcataataaa
     2161 ctagtccttt tcctttcatt gattttctgt cttattcagc attcattaga cattttttgg
     2221 aacagataat cgtgtcgaaa agctttttct tttcataaat ctgtctgtgt attctaaata
     2281 aaacaatttg taaataagca acgagcattc attcgttttt cattgacgtt cattttattg
     2341 catagaccac ctttgtttgg ggataaacaa gtggggataa aaatggttat gcatatgtaa
     2401 attataccaa agtttggaaa gtatcacaaa aatttataaa aataatgatc ctaaaaagaa
     2461 aaagactgaa atatgtgata aacatctcat acttcagtct ttttcattaa ctattcataa
     2521 gacattaggt cttcatcaaa cccgctaacg agagtagtaa tcattgctac gactagacta
     2581 acagccagtc ctgctaagta ccagaaaaaa gtaccggaaa tgaatgcagg caatgtggtc
     2641 agactaccaa atacaaaagc gatcgctttc acttgggcaa atcccattac agctccacca
     2701 gcagctgcac caatgcaagc agcaacaaat ggttttttca atgggatcgc aattccgtaa
     2761 acagttggtt cggtcactcc cgcaagtaaa atcgaagcaa aagcagaacc ggagatcgaa
     2821 cgagtttttg aacgtttact tcgtagaaat acacctaaag ctgcaccagc cattccaaaa
     2881 ttggctgctc ctgtcaacgg aacgattcgg tcgaacccat aagcagagat attactgatc
     2941 atgatagggt ttacagccca ttggatacca aatattacca aaatattcca accaccagcg
     3001 atgattgcgc cgctgatcgg tccgttaata tccatcatcc aagtaattgc ggcagctaaa
     3061 ccttcgccaa ggtaaacacc cgcaggcccg aataccatta acgtcaaagg aaccgtgata
     3121 actaatgaaa gtaaaggtac aaatactaat tgcatatttt cgcgaatata ttttttgagg
     3181 aatttttcaa caaaagaata aaagaaaata cctaaaatcg ccggcatgac agtagacgta
     3241 tactgcatca gtactacagg aatccccaga aaatcagtag ctgctttgcc actgtctgaa
     3301 agtaaagagg tcagattcgg ttcaatcagt gctgcagcaa tgacagccgc aatatatcca
     3361 ttcactttaa acgttttggc ggcagaaaaa gcaagaaaga ttggcagaaa ataaaagacg
     3421 gcattcgacg cagcagacaa aatatgataa gttccgcttg tttcactaag ccagccaatc
     3481 gtagtcgcta aaacaaccag accttttaat ataccagaac cggcaaacag cggaatgatc
     3541 ggcgtaaaga tttccgatat caaaccaaat agtttttcga tcggatttag cttttcttta
     3601 ggagctgaat gatcgatttg acgcgttaat tccttgtaag caggagccaa tccctgatcc
     3661 atgattattt ctgcatatct atcgagtaaa atgaaatact taatggaagg gatttgctgt
     3721 tttaattttt ctgcattggc tactggctgt tttttcaatt tgatacgaag ccgagtcgca
     3781 catggaagga cagaatcaat attgtctttt cctcccaact gttcaatcag catatgaatc
     3841 tgctcggaca gtaccatctg ttcaggattc attgtcgttt ccattagaag accactccac
     3901 tcactaatat aatattggcg taaggtgtca tttcgcctgt acgaataaat gcatgacagt
     3961 tattcaactc ttttttcatt tcttcatgtg ggataaagct aatcggtgta tctggtaaca
     4021 ataatttgat tttttccaat atttccgggt tcattgtctt tatttcttcc gccaattcaa
     4081 tcgattgtac ctcaagttct tctaaaacgt tttctaaaac agataaaaag cttggaagac
     4141 cgtttgagac agccaaatct attttttcac taccaaaggg gacaggcatg cctgcatccc
     4201 cgatacttaa tttatcaaag tgccccattt gtgcgattac tcgtgaaata tctgaattga
     4261 ttacttttcc ttttttcatg tttgtttctt cctctcattg ttaattttag actgctgatt
     4321 gttctaaagc ttcgcggtaa gggatagaag gttgcgcacc ataagactgc acagcaacag
     4381 aagaggctag atttccataa aggatcgctt ctttcaagtt actaaggtct ggctgaagtg
     4441 tgctgcttaa tgcgccaata aatgtatcac ctgcggctgt tgtgtcaacg gcatccactt
     4501 taaaagcagg gacgataccg tgttcttttt ctgtatgata gaaagcgccg gcactgccta
     4561 gtgtaatgat tactgttcca atacctaatt catggagttt ttcggctgct gcgaccaaac
     4621 tgttatgatc tgtcactctg attcctgtaa tgatttctgt ttctgtttca tttggtataa
     4681 tgatatctgt tttttcaagt agttcagtag ggatcgtttc acgagctggt gcaggattca
     4741 ggatcgttgt ttttcctgcg tcttttgcaa tagaaaaagc taggatcgtc gcatctaagc
     4801 tactttcaaa ctgagcaata ataaagtcac tgtttgtaat cagttgcttg ttttttagga
     4861 cttcttgttc atggaaagca ttatttgcac ctgcatggat caagatacta ttttctcctg
     4921 ctgcgtctac cataatcatt gcttgtccgg ttgtggcgcc ttggatttca gctatcccag
     4981 atgtatcgat gttttctttt gtcagtagat ctaataactg ttgtccttcg ctgtcagccc
     5041 caacgcctcc aataaaagat gtccgtgcgc ctgaacgttt ggctgccact gcttgattgg
     5101 cacctttacc tccgccagcg tgaaaaatct catgcgcatg cattgtttcg cctggcttgg
     5161 gcatgttggt taaacgaagg gtagtgtcca tattaatact tccaattact gtaaccgtgt
     5221 tcattttaca aacctccaac tatctcattt attttgatgg gaaaaattat tttttaaaac
     5281 gttttagtaa aacgttttaa tagttcgata aaaatatagc acaccttgaa agctatttca
     5341 agggtgcagt tgaaaatctt ttttctaatc gtacaggtaa tcttttttct tcccattctt
     5401 tttctgggaa ttggatccga tctaacaata atttggcaga tgtttgtccc aattcgaaga
     5461 tcggttgtgc gatcgttgtc agtttcggct tgatatactc gcacatatct atattatcat
     5521 agccaataat gctgtagtct tcaggaatgc tttttccaga ttcctctaat ccgcgatata
     5581 atccaaaagc taattcatca tttaaagcaa acacaccagt tgcagcagaa tcaagaagtt
     5641 ctgctgtgat ttggtagcca ccttgttttg agaactgtgt aggtaagagg atcagttggt
     5701 cgtgagagat ttggtaaaca tcaagagcag atttaaaccc ctcaatccga gcgtggacat
     5761 tttccggcgg attttcagga tagacaaggg cgatcgtttg atgacctaga ctaagcaagt
     5821 gcatgcccgc taggtaacca ccgcgaaaat cgtctgttcg tacggcatca ctaaatcctt
     5881 cagatttttt ctgatccaaa acaatgaagg gccggccttg ttttttcaaa ttttcgttga
     5941 ttgcatctgt agagacagca gaagtcgcga taatgaatcc atccacacct cgacgtgtga
     6001 gctcggctag atattcgatt tctttttgat gatcagaatc agcattacat aatatcgtga
     6061 cgaaattctg cttatacaag atatcctcaa ttcctcgcat cagtgtactg aagaatggat
     6121 tcgtgatatc aggaacgagt acaccaattg ttttcgtttc cttggtaatc atttgtctag
     6181 caaaatagtc aggctgatag cctaattctt ctttggcagc taaaactttc tgaacagttt
     6241 tcggactgaa tttctccgta tttccattta atatcaagga gacagttgtg attgaaacgc
     6301 cggaaagttt agcgacttct ttgattgtga tctttttttt caccatgttc atactcctat
     6361 ctaaatcctg tggttatttt cccatgaaat caaaaagaag taaatgaaaa tcacgtgtgc
     6421 cagtacttat ttgctttgtt ggatagcttc gattatttgt ttcgttgagc gtactcgtgc
     6481 agttttcgga aatatctttt ttatgaacaa ttgatgggat tctttgtcac gatcactgca
     6541 agcatcttca gcaagtacaa catgataccc atgttggaag gcatctaatg cagtagcata
     6601 tacaccgttt gttgtcgcta caccgcttaa aacgatagtt tcgattccac gccggcgaag
     6661 ttgaagatca agagaagtac caaaaaaggc acttggattg tattttgtga tagtcaacat
     6721 attgtttgta tctttcaagg aatccttcaa caacaagtca gtgaattctg ccggagcaga
     6781 caaagcagat attcctccag catctgtctg atgacttaga taagagaaag tagtgatgtc
     6841 tacattcact aaaactttca aggcagcggt acgtttcaat gtttttgcta acaaatcatt
     6901 gtttgtcaga atagattgtg cagtgtgtgg agagagtgta ccgttattta cgatcccttt
     6961 ttgcatgtcg atcgcgacaa aagcagtttt tgataaatca aaaatcatat tttcctttaa
     7021 atcattcatg atgttccttc tttcaaataa caatgttttt tatgtaagta aatcataaat
     7081 cgtttatttg tcaactaaat aaaggattcc tttctttagt ctagagaaat tcagtggtaa
     7141 aataaataag tatgagaaaa cgcattcgat gcatagatat agcgagaaat atcgtgaaaa
     7201 tggaggaatc agagtgaagt atgtcgaagt aaaaaatgaa tacaaacgat acaaaatggg
     7261 agaaacgacc attacagcaa atgacgatat ttctttttca attgaaaaag gcgaattagt
     7321 gattatactt ggtccaagtg gagcaggaaa gtcaacgatt cttaatattt taggtgggat
     7381 ggatacacct gatgaaggtc agatcattat tgatgacaca gatattgcgc aattttcaga
     7441 taaacagctt actgcttacc gacgaacaga tgtagggttc gttttccaat tctacaatct
     7501 cgttcctaat ctaacagcaa aagaaaatgt ggaattggca acggaagttt ctcctgatgc
     7561 gcttgatcct gtagaggtat tgaggcaagt gggcctagct catcgattga ataactttcc
     7621 gtcccaatta tcaggaggcg aacagcagag ggtttcaatt gctcgtgcac tagctaaaaa
     7681 tcccaaactt cttttatgtg atgaaccgac aggcgcgctg gattttgaaa cgggcaagca
     7741 agtattgaaa ttattgcaaa acgctagcag acaacacggc aataccgttt tgatcattac
     7801 acataattct gctttagcgc ccatcgctga tagagtcatt cacattaatg atgctaaagt
     7861 aaggtctgtt gaattaaatg atcatccttc atccattgat gagattgtat ggtaggggga
     7921 ttttatgaaa acaagaacat atcttaaagc aggttttcgc gaaattcggc aatctaaagg
     7981 acgattcatt gcaatcatcc ttattgtcat gctaggaaca cttttatttg taggtgtcaa
     8041 aactgctgga cctgtcatgc aaaaaacgat ggaccactat gttggtacag caggactttc
     8101 tgatttgcaa atcgtttcta ctggtggctt gacacaaaaa gatattgctc aagcagaaaa
     8161 gatcccagat gcaaaagtag aaacaggaaa acagatctat tattccaata ctggtaaaaa
     8221 cgaagtgatt caaatatttt cttataacaa aaataacaag cagaacaagc tacaattaac
     8281 tgatggaaaa ctcccggaaa aaccaaacca gcttgtattg gatgaaaaag cggaagatga
     8341 agggtataaa atcggtgata tttatcagat tgattcagat gaattgaaag aaaaagagta
     8401 taccatcacg ggctttgtcc gttctccatt gttcatcaac aatttggaga gaggatacgc
     8461 gaacgtcgga aatggtagcg tcgattattt tgtctattta cctgaatccg tttttaaaac
     8521 agatatttat tctgtcttat atcttgattt tacaaacgta aagaatttga atacgtacag
     8581 caaagcttac aaagataaaa tggaacacaa tcaagagaag gcagaaaagt atttacaaga
     8641 tcgccctgaa gaacgcttag aagagctgaa aaaatcggca aacgaagaat tagaaccagc
     8701 acagcaaaaa ataacagaag gcaaagcaca aatagcacag ggaagaacac agttagaaga
     8761 tgctaaaaaa caattagcgc aacagtcagc agctatccag cagcttcctg aagcgcaacg
     8821 aacaatcgct gttcaaacat tatctcaaca agaagctcaa cttgctgaac aagaaaggca
     8881 attagttgag aaagaacgtg aactggcctc tgcagaggaa gaattgaatc gaaatgcaga
     8941 aaaaattgaa aatttaacta aaccatcata cctatacaat gaacgttcag aaaatgtcgg
     9001 ttttcaagaa tttggtgatc tgtctgatcg tatcgctgcg attgccaatg tttttcctgt
     9061 tttctttttc tttattgccg cattgatcac ctttacgaca atgacacgta tggtagaaga
     9121 aaaccgcagg gaaatcggga cattaaaggc gttaggttat acgaaagttg aaatcgcaaa
     9181 aaaatatgca atctatgcaa gtctagcctc agggataggc attgttttag gaacgatatt
     9241 aggaacgaat cttttgccaa ggatcatttt tgagctgtct aacgagcggt atgatattgg
     9301 gagtgcgctt attttttatg actggccgcc gattttacaa gcagctgccg cattcttcat
     9361 tgccgcattt ggcgctgcaa tgatcgtttt attcaaagat ttaagagaac ggcctgctgc
     9421 attgcttcaa ccgaaagcac cgaagccagg gaaacgcatt tttcttgaat acatcactcc
     9481 gctgtggtca cgccttagct ttaatcaaaa aatcagttac cgaaatctct tccgatataa
     9541 gtcaagaatg cttatggcga ttatcggaat tgctggatgt gccgggctga tggttgcagg
     9601 cgtgggattg aaagattctc taagttctgt ctcagctaaa caatttggac cgatcattga
     9661 ctatcaagca atcgttacat tggattcatc cacggaagat agtcgtcaaa aagcagcgga
     9721 tatgctgcag aagcaagaaa aagtaaatga gatcctagct attcatactc aaaccgtaga
     9781 attgagaaaa aaaggacatg cttcgcaaag tctgacagtg atcgtaccaa gtaaaacagc
     9841 tgatttggat cgttttatcc atttaactga tcttgaagag aacccacgct ccttgcctaa
     9901 aacaggaatc gctattacgc aaaaagcagc agaactattt gacttatcta caggagatca
     9961 tatttcgctt tatgatgaag atcaaaaaga atggaaagtc gaagtaaaag aaatcgtgaa
    10021 aaactatcta ggtagcttta tttatatgtc ccctgaatat tatgcttcag ttactgatca
    10081 atcgatgatg gataatgcat ttttgctgcg tacagataag atgacaacaa aagaagaaga
    10141 aatgctttcc gagaaactgc ttgccacaga cgctgtgaca aatacgtctt tcatgtccaa
    10201 acaaatcgaa acgcaagaag aatcaatggc gaacttagac tcggttgtct taatctttgt
    10261 cgtattatca ggactgctgg cttttatcgt cttgtataat ctaactaata tcaatatttc
    10321 cgagcgtgta cgcgaattat ctacaataaa agtccttgga ttttttgata aagaagtcac
    10381 gatgtacatt gttcgagaaa atatcatctt tactctttta ggaatcatcg gaggatttgg
    10441 tgtaggatat atattgactg acttcatctt gcaacaagcg tctatggaga atgtcatctt
    10501 tccattagtg atcacatggt ggtcttatgt cttatcagca ggattaacta tcgtatttac
    10561 ggttattgtg atgattgtta cccatttcaa attaaagcat atcgatatga ttgatgcgtt
    10621 gaaatcgaat gaataaaaac agaggttgtg aaatcagcgt tttgagctaa taacgagggt
    10681 cagctgctaa ttgaacaccg tgaatcagag gttgtgaaaa agctgtcctg catcgagcaa
    10741 aaggaatact caaataaaat tagctcctca tgttttacat ttgagaagac tgattgccgt
    10801 agataaaagc ttttgagcac catttataga agaaacaaga atattttgct attttaaaca
    10861 agaggataga caaatacatg tctgatctct tgttttttat tgggcaacag acctcttaga
    10921 atgtataaag ataaatttta caaacgcttt ctgttcagtt ataataataa atgataactt
    10981 gtatatataa gttgcggtag gtaataaatc agttgaatgg aggtaaatat ccatgacatt
    11041 taaagacaaa gtcatttacc aaatctatcc caaatcttac aaagacagta atggtgatgg
    11101 aattggagat ttaaaaggaa tcaaagaaaa actcccgtat ctgaatgatc taggcatcga
    11161 catgatttgg ctaaacccta tttatccaag ccctcaaaaa gacaacggtt atgatatctc
    11221 tgattacaca gccatcgatc ctatctttgg tacgatggag gaattcgaac agctaatagc
    11281 cgaagcaaag aaataccgga tagaaatcat gctcgatatg gtgctgaatc acgtttcgac
    11341 agaacatgaa tggttcaaaa aagcactagc aggggaccag cattatcagg acttcttcat
    11401 tttaagagaa gagccaacgg attgggtatc aaaatttggt ggaagtgctt ggtcgccatt
    11461 tggagatacg gggaaatatt atttgcactt atatgatcgg acacaagctg acttgaactg
    11521 gcggaaccaa gcagtaagag aggaactatt caaagttgtt cgtttttgga tggaaaaagg
    11581 ggtaaaagga ttccgtttcg atgttatcaa tgttatcggt aaagacgaac aattgaaaaa
    11641 taacaaagaa aatgatggca aacctgaata tacggacaaa ccaatcaccc atacgtatct
    11701 aaaagaattg aaccaagcaa cgtttgggac ggattccact atcatcacag tcggtgaaat
    11761 gagctctacg acgatcgaaa attgtatctt gtatacagaa cctagtagag aagaattatc
    11821 aatggtcttt aatttccatc atttgaaagt cgattatgaa aatgggaata aatggtctac
    11881 tcctccattt gattttgcag aattaaagcg actatttcat acatggggag aaaagatgag
    11941 tgagggtaac ggctggaatg ctctattttt taataatcat gatcagccac gtgcgctcaa
    12001 tcgatttgta gctatcgatc agtatcgaaa acaaggagca gagatgctag caaccatgat
    12061 ccatttgaac cgtgggacgc cctatattta tatgggagaa gaaatcggca tgatcgaccc
    12121 agactattca tcgatttccg actatattga cgtagaaagt ttgaatgctt ataagttact
    12181 ccttgaacaa ggattcacag aaaaagaagc gtttcgtcgg atcaaagcaa aatctcgcga
    12241 taactctcga acacccatgc aatggtcaga cacagaacaa gcaggattta ctacagggaa
    12301 accttggtta aaagttggag gcaagctaga agagattaat gtagaaaaag aactatcttc
    12361 tgaagattct attttgtcct attacaagaa attgattcgg ctgagaaaaa catatccaat
    12421 cgttgcacaa ggagattatc atgcttatga agcaaatcat ccggaagttt atggctttct
    12481 tcgccaattt gaaggacagc agctgcttgt attaacaaat ttctatgata aagatacaag
    12541 gatcaccatc ccagaaggat tcacagaagc caatattctt atcgataatt attccgatac
    12601 tgttcttcat caaagtctgg agctaaaacc ataccaggca attgctttgc tgaaaaatta
    12661 aaacaaaaac acaaaacgtc gttcctacga gctattctcg taagatcgac gttttgtgtt
    12721 ttatttaaaa taatcttctt cagaaaatga agagttttca tactctcctt tatcgccata
    12781 aaatttattg tagcgttgtt tgaatttacg gaaaataaaa gtaacgagta atttgactaa
    12841 cgtatagata ggaataccaa aaataacgcc cacaattccc attagatcac ccatgacaag
    12901 taaaacgatt aagatcgtaa tcggatggat ctgtaaacga tcacccatta ctcgaggaat
    12961 caccaagtca ccgtggagaa gttgtacggc aaaccagaca atgacaaatt tgacagccat
    13021 aaaagtagag tcttggaatg caatgatcaa acctggaaag aaggcgataa acggcccaat
    13081 atatggaatg atacataaca acccagcaga aatagctaaa acactagcaa attctaatcc
    13141 gatcagtaag aaacaaaccc agtagactgc tccaaggata aatgaagcaa tgatttgccc
    13201 ctttagaaat gaacccaatt gttgattagc aatctttgtc aaatttttaa aatctgtacg
    13261 aaatgctggt gggacaatcg ctaaaactga ctgataaaat ttttgaggat ctctcaaaag
    13321 aaaaaaagcg acaattggac cagtaaataa agtaataaaa atagctgtaa cagttgaaaa
    13381 aatgttgctg atccgttgtg cgccactttc ccaataattt ccaataaatc cagctatatc
    13441 agacgtcacg tcgtcaatag aagcgaaaaa ttgattaaat gaatcggcaa aaggtgtttt
    13501 ctctaaaaat tgtcggagac tctcttgaaa atcttgaaac aaatctggca aactactgat
    13561 caatgtcttt gcctgttctt caagaaaagg aacaagccat acgcttccaa gccccaataa
    13621 aaataaaata atcacataaa cgataatgac tgacaagttc ctcgaaaggt gcttgctcaa
    13681 acgtttgatg actggttcca aaagataata caagatcagt gcaaacaata atgggggcaa
    13741 gatagtagcg ataatgattc cgacaggctt gaatataaac gagatctgat ggaaaaaata
    13801 aatgatcagc gcaagtaaga taactattcc taaaacatag taagaggttt ttcctcctaa
    13861 aaagcggatc aaacgattcg ttgtatctgt ttcttttttc actctttttc ctcctttatc
    13921 tataacttca ttatagacaa aagcgggtat aaaaggaaaa tgatagaggt tgattgcaaa
    13981 aatatcgtca atcattcata taaactttct catcaattaa aataagaaaa cgcttgacag
    14041 ttcttttaac catatgataa attaaatcct gttgctaaaa cgtaactatt acgttttgta
    14101 aaaggcgaat cggaacattc aaatgaaaga agatgagaag aggagtgtca taaatgaaac
    14161 gattgatagg tatattggcc atgctggttt tagcaggtat gttaactgct tgcggcgggg
    14221 caagtgggaa agccgaggac agtaaagaaa aattgagcgt gatgacgacc ttttatccta
    14281 tgtatgattt tacaaaagca atcgtcgggg atgaaggaga agtagagtta ttgattccag
    14341 cagggactga ttcccacgat tatgaacctt ctgctaaaga catggcaaag atccaagata
    14401 cagatatctt tgtttataat gatgaaaaca tggaaacatg ggtccccgca atccaaaaaa
    14461 cattacaaga aggcaatgtc catacgataa aagcgacaga aggtatgctt ttacttccgg
    14521 gaagtgaaga aggacatgat catgaccatg aacacgggga agaaggccat acacatgagc
    14581 tagacccaca tgtatggcta gctccaagtt tagcaatcaa gcaggtagca aacatacgtg
    14641 atcagctgat tgaagcttac ccagaaaaac aggaagtatg gacaaaaaat gcagcagcct
    14701 ataccgagaa acttcaagca ctccatcagc tttatcaaga aacctttaag caagcaaaac
    14761 aaagatcttt tgtcacgcaa catgctgcat tcaactatct ggcattagaa tatggattga
    14821 atcaagtatc gattgctggt ctttcttcta gtgaagaacc gtctgcggcg agaattgctg
    14881 aactgaaaca ctttgttaaa gaacatggaa tcaactatat ttattttgaa gaaaacgcca
    14941 aagatagtat tgccagaacg ttagcaaacg aagcaggtgt atctttagaa gtattgaatc
    15001 ctttggaagg attgacgaat gaacaaatag aaaatggaga aaactatctt tctatcatgg
    15061 aagcaaacct cgaagcgttg aaaaaaacga cagagacaga aaacccgcta gaagatactt
    15121 tgacccccaa aaaagaaaag agcgtctaca acggttattt tgaagacgag gatgtacaag
    15181 atcgctctct ttcagattgg gcaggaactt ggcaaacagt agatacgtat gtagaagatg
    15241 ggacttttgc tcctgttttt gaatataaag aaaaattgaa tcaggataag acagcacaag
    15301 aatataaaga atattatacg aagggctatc aaacagatat tgcttcaatc aagatcagtg
    15361 agaaagagat ggcatttacg tttaaggatg gaacaacgaa acgttcaaat tatcgttatg
    15421 tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg cgtaaggtat ctattcgaag
    15481 cagaagataa ggaaagtggc gcttttagat atattcaatt ccgtgatcac acgattgcac
    15541 cggctgactc cgaacatttt catttatatg caggaaatga aagtcaagaa gcattgctga
    15601 aagaaatgga gaattggcca acattttatc cagatacaat ggatggacgt gaaattgcac
    15661 aggaaatgct tttgcactga taggcaaaaa agaaccgaag ggtttggatc ggctagtcat
    15721 ttagtcggtc caaactattc ggttcttttt atgaaatcaa tttctttaat ccacggatag
    15781 aggctgggat ggtctcgccg tttgccaacg tgacttctct ggttttttta ttaatcgacc
    15841 gcacattttg tgtattgata acataggatt tatgacaacg gataaaattc cgatgcattt
    15901 tctcgatttc tttgatcttc tcatagaatt gtaattgggc atgagtcaaa tggacttcca
    15961 gtttatgtgg tgctcctagc gaagtagaaa aatattggat gtctttgatc ggtaaaaatt
    16021 tgatctcatc attaaactct attttgaaga tatctttttg gatctgggac atttgaaaat
    16081 cttcgaaaat tttgctcagg atatgctcga cattttcttt taaatcataa agatcttctt
    16141 tgacgatata atcagtcggt tcgatgttgc tttttaagat tttcatcgac atttccgtat
    16201 gggaagtcac atagattatt tttcctaagg gatctaattc acggatcaat ttaccgatcg
    16261 ctacaccatc catttcactg tcttctagtt cgatatctaa aaagtaaata ccaaaagaag
    16321 aagttctgat cttggctgca ttgagaagtt cttccggatc agaggtagaa atatggacat
    16381 aactgtctaa gttttcaatc atgattctat ttttgatcac cgttgataaa acttctaatt
    16441 gttttggatc gtcttcacaa atataaatgg gaatcatatc attactcctt tattttattt
    16501 gtatataaat cgtttgagaa aaacgctggt tttcaattga tgtttcaagg aaaagataag
    16561 gattggtctt tgtcaattca tccaagataa acaagccaag gccttcgttt tttggatttg
    16621 tttttgttga gaaccctttt tgtttcaact ggtttaacgg caatgtattt gttgttgtat
    16681 tgatcacacg aatcatcacg gaatgattct tcttgagtat ggtcaattcg atttttttct
    16741 tctttgcagc actagctcct tcaatcgcat tgtccaagat aataccaagc atacgtacaa
    16801 ggtttaccgt atgtttcgta tccagttcga ttggctcagg aaaatgaagc tggacgacca
    16861 accctttttc ctgtgccatc atgagtttta atgacagtaa actacggatc tcaggaactt
    16921 tcatattatc caacatactt agattggccg gaaaaaggtt catcatctgg cgtgtaggta
    16981 atatcgtttg agcataataa gaccgtaatc cttctaagtc gccttctgaa atatagctgt
    17041 gaagagaaaa aagcagattt tggtaatcat gtttgaattc tctcaagtct tgatagtttt
    17101 tttctagatc tttgatataa tcctgttgca tcctgacttg ttgctctaat aactgatcag
    17161 ctaatttctt attagagatt ttccaataat aaaccaagat gatcaggcca acacacagat
    17221 atagaatgaa aaaactcgta ttcaaagcta agacatcagg tgtttcacca gaaaaacggg
    17281 tataaaaaat aatgatatag tatgtaaata atatcaatgt cccgatcgta ccgatcatac
    17341 ggcggtccac agtgtgaaac caagtctcta acgatttttt aaaacaaaaa gctaataaag
    17401 cagataaaaa agcggataag gcaatgtgaa aatactgcaa tgaatctcct ggttcaataa
    17461 ccgaagattt caaaacaaaa aaatcgccga tgcttgttaa gtgatctcct aaaagcgacg
    17521 tcaataaccc aaaaggaagc gcgtaaacca attgttgacg cgaagtcgaa aagaagaaaa
    17581 aagtaagcat cagcattcct atagcaatta gagcagtaaa aatccctaaa atcgaataga
    17641 cccatccaat caaaatacaa gaaatcagaa tgactcccgc aaacagatat ttgtatcgga
    17701 tgtgatggtc aatcaataag atacaaattg tgtaaatcaa atattgcaac gctatggacc
    17761 ataaaaacat aaagtacagc tcccattgat ttttaccaaa ttatagcaca attagcgtat
    17821 tattttttta atttttcagg gattttgaat tcagaactca taccaaagca gcagccagat
    17881 acaaaaaagt cagataagtt agttaattgt ttaatgattg tttttttcat gaaagattcc
    17941 tccaaaattc atcaagtagt ttgtaatagg aagcaacgta gctatttcta aaacaatccc
    18001 aagtagcaaa taggtagaaa agtaagaagc tgagaaaaat aaagtaatga gagaaaaaat
    18061 tagaaacatg ctgatcgcct tctttaataa tgggggacgt ttctcgggtt caatgggatt
    18121 gattgctgta cctcttgatc catagcgaag cagtaacaaa aagcagctga ttaacaaaaa
    18181 gaccatcatt gggaaggaga tagtcaattg cagatgtttc atcaccaagg gaacgcccca
    18241 taaataaagt agtgtccaca gcagacaaac gaattcagag tcggaatgca atccataagc
    18301 atatctgcgg atgcatatat aagaaagaaa ggtgattcct gtttccgccc atgagcctgt
    18361 caccagagaa aagactagaa cgaccgctag ttttccaaga ttattcaata aaacttctaa
    18421 ggcaaatttt acttggacat acaccatatc ttcttccgat ttgtctccgc tgaataaggc
    18481 ttctaataat ttgttcacaa ctttatcttt catctcatac gtacctctac cacaaatttt
    18541 atgctaaaaa aaataaaaaa cgtcttttgt ttcataaaaa gtggttttga catcaaaacc
    18601 gtcatgattt cctaaaaaaa tcttcgtttt ccaagaacct taccaaaaaa agtgacagtt
    18661 ttgatacgaa aagtactttt tatgacaagt ttgtgctttt tcgagtgatt tatgttaaaa
    18721 aaattactga ccttataaaa ggtgatggag ggattcttat ggaaaatgaa caattgtttg
    18781 aagtcggcga aacatatgca tttaaatctg atttatttga atcaccaata aaaggaaaaa
    18841 tagaaaaaat ctatgaaaat agtgtgatga tacgtgtcat gaaatgcgca gtagaagaca
    18901 atgataccgc tcataatctg cactggcagt tagttgtacg caaatcaagt acaatagaag
    18961 aataaaaaca gataaaatgt tgagagcata aagaggttgt gagtctccgt ttagctctga
    19021 gttacaagga ataatgttaa aaaacagctt ttcatgtttt atgaatattg ccacttgtga
    19081 ccgaggtgtt ggcaggagaa tactgtttat ttgaaaaaag ggctgcgaca agacttttgt
    19141 cacagccttt attttcatga ataggagtta cttttggcta cttttagaga agtagctttt
    19201 aaggagcaga taaagtcaag gcttttattt ctataaatga agtagtaaaa tgaagaacag
    19261 attttattac aggaaggatg ccgataattg tgatcattgt tgcaggaacg atcggtgcag
    19321 ggaaaagcac actgactgaa atgcttgccc aagatttaga aacaaaacct ttttatgaga
    19381 acgtggaaga taatgaagta ttgccgctat tttattctaa tccagaaaag tatacttttt
    19441 tattacagat tttctttttg aacaaacgct ttttggcaat caaagatgct ttcagccatg
    19501 atgacaatgt actagaccgc tccatctacg aagatagtat gctgtttcat ttgaatgcag
    19561 atttagggcg tgtttctgaa gtagaagtaa agcagtatga aggtctttta gaaacgatgc
    19621 tgaaggagtt agaagaaatt tcgccacaaa agaaaccaga tctactggtt tatattcgtg
    19681 tatcttttga aactatgtta gcgagaatca aaaaaagagg ccgggaatat gaacagctag
    19741 aacaagatcc tgaactctat tcctactata aagaactcaa tcgacgatac gaagagtggt
    19801 atgaacagtt tgatatatgt cccaaaatag tgatcgatgg ggacaaatat gatttcgtag
    19861 ccgatccggc atgtggagaa caaatcgtac aagaaatcaa gatgcgggca aagaaaatga
    19921 cagaagaggc agatgcccag tcgatccgct acactacaaa taacccgaaa gcaaagggaa
    19981 tctttacaac taaataatat aaaaactaag caaattgctc actcgcagat atttctctgt
    20041 gagcagcatc ttgcttagtt tttttgattc attgagatca aatagtgaac cgattcgtat
    20101 gataaaattg ccataaatga aatgtctgag tgagggccat gatcaaaaat aaaatggaaa
    20161 cgatcaaaat cgaatgggcg ccaaacagat gatgacagaa aacaaggaca atagctcctg
    20221 cgataaaggc aagaacgaca cttagatagt ggaaaaatcg atcaaaatct gctttttctt
    20281 tagaaataca ataatcatat aagttcgttg ctaccccttt taaatttcca gttgtaaata
    20341 aattagtgta ggcacgcccg ttgattttat caaaagctac ccactggatc gatgcagtaa
    20401 aagcaatcaa aatcgttact aatgcgggat gctcatggac gggtgtcaaa ctaacaaaga
    20461 aaaaaatcac tgcttcataa tatagaacaa acaagcgcca gaaatgtttt tctttttttc
    20521 taaaataatc aattagatac tttgttagaa gaatccctaa gaaaaagaaa agggtagaca
    20581 atactttttt accaacactt agccattctc cgtcaaatgc ttggatgatc gatagaatga
    20641 tattgccagt ttgagcagag gcaaaagcac catattggat ataagtataa ctgtccatgg
    20701 ctcctccgat aaatgtcagc aaaagtccta ttgtacgccc ctcgtgaaag gaagtctgca
    20761 tttttttcat caccttttat gaatattttt gtcttcctta tcttagcata aacgattgaa
    20821 aaaaaggaaa aattttcaga aaactaaaat catatcgctt cccattttca tttttagaaa
    20881 ggtctgttag tataaagaaa aaatagacaa cgaaacttta aataaccaac ttgaaataac
    20941 caacttgaaa taaacaaatt caaagaagaa agatgggaag gaaatcaatc agttgaatga
    21001 aaaagaaatt ctccgattac aaaaagaatt gaaacgctgg gagaacaagt ataaaaagct
    21061 tcaacaagtc tttcggctac aagaagaaat cattgctacc tatgaagcgg cttatggcga
    21121 agcacccgga cttattgagg aagaagtaca agtaaaagaa agtgaaggaa acagctcttt
    21181 ctttaaaatg atggagaaac gttcctataa tcgcttcaat caagaacaaa aagcagctat
    21241 cacatatgat atgaagaaac atcttcgaat catagcagga gctgggagcg gcaaaacgca
    21301 aacaatctgt gcaaaagctg tttatctgat gacgcaaaaa caagtagacg aagaacgcat
    21361 actaatgatc acttttacaa ggaacgctgc aaatgaacta aaaaaacgag tagataattt
    21421 cagccagaga aaaaccgctg tccatatcgg aacattccat agtatctttt ttaggatata
    21481 caatgaaatt tgtcgaaaat ttcctgaagt agcaatgcaa ggaatacaag gagacttttc
    21541 aaaggatact gcacaaaaag tgaatgctgt tttgcaacag ctgatacgta aatataatct
    21601 ttatatattt gatcaatatg gagaaaaaac gattgcttct cggttagact attggcagaa
    21661 tatgaacctt tcagtagaag aaatggttca gttagttaaa gaaaaatatg attccattga
    21721 taagaatgca cgacagccga tatcagaacg actgtatgga ttgctcacgg aactccaaga
    21781 acaaaaaaga agccagcaat tgctagagtt caatgatgta ctccaaaatc tgaaaagcgc
    21841 tttggagaat gaggagatcc agcattatat cggccaaaaa tatgattatc tattcattga
    21901 tgaatttcaa gatactaatc cacttcaatg ggaaatcgtg aaacggatga caaaggaaaa
    21961 caggatcaag ttgattgttg ttggagatga tgatcagagt atctatggat tcagaggatc
    22021 agagccagca tacatcaaga actttgaaaa agaatatcct accaaaactc ttttcttatt
    22081 aactaattat cgttctcggg cagctattgt ccaaggagca aatagattga tcagctataa
    22141 taaaaatgat cggataccaa aggcgatgat tccagcacaa aaagaagcag gtgttatgga
    22201 agcctatttg ttttctgata ctaaagcaga aagtcagtgg ctaatcaaac aaattcagac
    22261 atttattcaa aaaaatggga aatataagga atcaatcata ttataccgat cagcttcaca
    22321 aacgcagcag cttgtccaat ctctgctgaa aacgaatatt ccttttgtac tggaagcaga
    22381 tagtccatat gaaggtatct ttggtatcaa gtctttccaa catttctatc aaaaaattgt
    22441 tcattggcaa acagcactga atctgcaaaa gaaaaatcag gcctaccagc aattgctccg
    22501 gcagctgatg gcagactgct acctgaaaaa aagtgaatgc gatcaatact tttctcctaa
    22561 atatcaaaat atagccgttg cagattatat tttgtcgata agccccaatt taaaatcaaa
    22621 atctgcagaa atccgagagt ttgaggaagc gttgcgtaaa acagaccagc atcaagaaat
    22681 ctattcgctt gttcaaagct atcttcatct gccacgtata gctaaggaaa tcgaccagag
    22741 tgatcaagaa tggatacgag aagaagtaaa gcagcatgct actttcagaa gactccaagc
    22801 cttatcgcaa gaaacccaag cgacagctaa agaattaaaa acccgcttga ttgcttacag
    22861 acaaggaaaa cttgatgcac tatgtatcca gagcattcac aaaagtaaag gtcttagtta
    22921 ccgaaatgtc tttttgattg gttgtaacga aggaagtttg ccttataacg gagcgacaga
    22981 aaagaaagta atcgacttta atgaaataaa agcagaacca gtgacgacga tagaagaaga
    23041 acgaagacta ttttatgttg cgatgactag agcaagaaat cgtctgtatc tatgtgtacc
    23101 tcaaatgaaa aattcaagaa aattgaaagt atcgagattt atcaaagaaa ccgggagtcg
    23161 tgtgaaaaaa ggaaagtgat agaagagagg gtgtgacaga agtggtcagt ttcaagaaag
    23221 aagaaggaat gtacgaaaat tgcttctcaa atttttgtga aatttcagct tattttcgaa
    23281 gaagctattt ctggaacacc gtttattcgg aaaaaggagg tgtgacaaga attttgtcac
    23341 acctccctta tttattttga aaatgagaat actattatca aaatagagaa tatatagaat
    23401 aattctcatt attcaaacgt aaataatcag tt
//
LOCUS       DACDXE010000044        23075 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.044, whole genome shotgun sequence.
ACCESSION   DACDXE010000044 DACDXE010000000
VERSION     DACDXE010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.044"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            101..2542
                     /locus_tag="IWM23_001722"
     CDS             101..2542
                     /locus_tag="IWM23_001722"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAP9884356.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2872..3576)
                     /locus_tag="IWM23_001723"
     CDS             complement(2872..3576)
                     /locus_tag="IWM23_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884357.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(3763..4008)
                     /locus_tag="IWM23_001724"
     CDS             complement(3763..4008)
                     /locus_tag="IWM23_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884358.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(4025..4876)
                     /locus_tag="IWM23_001725"
     CDS             complement(4025..4876)
                     /locus_tag="IWM23_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAP9884359.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            5042..6046
                     /locus_tag="IWM23_001726"
     CDS             5042..6046
                     /locus_tag="IWM23_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAP9884360.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(6287..6643)
                     /locus_tag="IWM23_001727"
     CDS             complement(6287..6643)
                     /locus_tag="IWM23_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9884361.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            7011..8198
                     /gene="tuf"
                     /locus_tag="IWM23_001728"
     CDS             7011..8198
                     /gene="tuf"
                     /locus_tag="IWM23_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAP9884362.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IWM23_001729"
     CDS             complement(8386..10977)
                     /gene="mprF"
                     /locus_tag="IWM23_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAP9884363.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11003..11722)
                     /locus_tag="IWM23_001730"
     CDS             complement(11003..11722)
                     /locus_tag="IWM23_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9884364.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            12042..12563
                     /locus_tag="IWM23_001731"
     CDS             12042..12563
                     /locus_tag="IWM23_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAP9884365.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            12622..13482
                     /locus_tag="IWM23_001732"
     CDS             12622..13482
                     /locus_tag="IWM23_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAP9884366.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            13589..15982
                     /locus_tag="IWM23_001733"
     CDS             13589..15982
                     /locus_tag="IWM23_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9884367.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            16164..18005
                     /locus_tag="IWM23_001734"
     CDS             16164..18005
                     /locus_tag="IWM23_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAP9884368.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            18029..19138
                     /gene="rpoD"
                     /locus_tag="IWM23_001735"
     CDS             18029..19138
                     /gene="rpoD"
                     /locus_tag="IWM23_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAP9884369.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            19358..21457
                     /locus_tag="IWM23_001736"
     CDS             19358..21457
                     /locus_tag="IWM23_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAP9884370.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            21653..22516
                     /locus_tag="IWM23_001737"
     CDS             21653..22516
                     /locus_tag="IWM23_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9884371.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            22621..>23075
                     /locus_tag="IWM23_001738"
     CDS             22621..>23075
                     /locus_tag="IWM23_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAP9884372.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         7770 a         4038 c         4810 g         6457 t
ORIGIN      
        1 ttaaggattg ttaaaattta atcttagaaa aaaataactg gaacaagagc tatttgtttt
       61 tacagcaaag aatggtatat ttttgtagga ggtggcttac ttgaaagaag tcgttatgat
      121 agatgctgca agaacgccga taggaaagta tcgcggaagt ttaagtccat ttactgcagt
      181 tgaattaggc acacttgtga ctaaaggatt attagataaa acaaaactga aaaaagataa
      241 aatcgatcaa gtaatatttg gaaatgtcct gcaagctggt aatggtcaaa atgttgccag
      301 acagattgct ttgaacagcg gtctccctgt agatgtccca gcaatgacga tcaatgaagt
      361 ttgcggatct ggtatgaaag cagtgatctt agcacgccag ctgatccaat taggtgaagc
      421 agaacttgtg atagccggcg gaacagagag tatgtctcaa gcacctatgc tgaaacagta
      481 tcagtcagaa acaaatgaat atggtgaacc aatttccagt atggtcaacg acggattgac
      541 tgacgcattt tcaaatgcac atatgggatt aaccgcagag aatgttgcaa cacaattttc
      601 tgtgagcaga gaagagcagg atcgctatgc cttgtcgtcc cagttgaaag cagcacatgc
      661 tgtcgaagcc ggtgtatttt ctgaggagat catcccagtc aagatttctg atgaagacgt
      721 gttatctgag gatgaagcag ttcgtggaaa tagtacattg gaaaaactgg gcacgttacg
      781 tacagtattc tcagaagaag gaactgtaac agcaggaaat gcttccccgt tgaatgacgg
      841 tgcctctgtg gtgatccttg catccaaaga atacgcagaa aataataatc tgccttattt
      901 agcaaccatc aaagaagtag cggaggtcgg tattgaccca agtatcatgg gaatcgctcc
      961 aatcaaagcc attcaaaaat tgacagatag atcaggaatg aatttatcaa caatcgatct
     1021 ttttgagatc aatgaagcat ttgcagcttc ttctatcgta gttagccaag aactgcagtt
     1081 agatgaagaa aaggtcaata tatatggagg agcaatcgca ttaggccatc caattggtgc
     1141 aagcggtgca cgtatcttga ctacattagc ttacggattg cttcgtgaac agaagagata
     1201 cggtattgct tcattatgta ttggtggagg tttaggcttg gcagtcttgt tagaagcgaa
     1261 tatggagcaa acacataagg atgtgcaaaa aaaaaagttt tatcagctta cgccatctga
     1321 acggcgcagt caattaatag aaaaaaatgt attgacacaa gaaactgcgt tgatttttca
     1381 ggaacaaaca ctttctgaag agctctccga tcacatgatc gaaaaccaag tgagtgaagt
     1441 ggaaatccca atggggatcg cgcagaattt tcaaatcaat ggcaagaaaa aatggattcc
     1501 gatggcaaca gaggaaccat ctgtcattgc agctgcaagt aatggtgcaa aaatctgtgg
     1561 aaatatctgc gcagaaacac ctcagcggct gatgcgaggg caaatcgttc tatcaggaaa
     1621 atcggagtat caagcagtga tcaatgcagt taatcatcga aaagaagagc tgattctttg
     1681 tgcaaatgaa agttatcctt caatcgtgaa acgcgggggc ggtgttcaag atatctctac
     1741 tcgggaattc atggggagtt tccatgcata cttatctatt gattttttag tggatgtaaa
     1801 agatgcaatg ggcgctaaca tgatcaactc gattcttgag agtgtggcaa acaagttacg
     1861 ggagtggttc cctgaagaag agattctatt cagtatttta agtaattttg ctacagaatc
     1921 attggcttct gcctgttgtg aaatcccatt cgaacgctta gggagaaaca aagaaatcgg
     1981 tgaacaaatt gccaaaaaaa tccaacaggc aggggaatat gcgaagcttg acccttaccg
     2041 tgcagcaacg cacaacaaag ggatcatgaa tggtatagaa gctgtagtgg cagctacagg
     2101 aaatgataca cgtgcggtta gcgcttcgat ccacgcctac gcggcaagaa atggattata
     2161 ccaaggatta acagactggc agatcaaagg ggataaacta gttggaaaac taacggtgcc
     2221 tttggctgtt gccacagtag gcggagcttc caatatcctg ccaaaagcaa aagcatcttt
     2281 agctatgtta gatattgatt cagccaagga actagcacaa gtaattgctg cagtagggtt
     2341 agctcaaaac ctggctgctt tgcgtgcatt agttacagaa ggaatccaaa aaggccacat
     2401 gggacttcaa gctcgttcat tagcgatctc aattggggct attggtgagg aaatcgagca
     2461 agttgcgaaa aaattacgtg aagcagaaaa gatgaaccag cagacagcta tacaaatatt
     2521 ggaaaaaatc cgggaaaaat aaaaaagcaa tatctttaga agaataaagc aagatttaca
     2581 gttattttat ttactgcaga tcttgctttt ttctctatca gaaaatatgt ttattattag
     2641 aattttttat agttaaggtt aggcatctta agaaatcagg caactccaag atatcttcag
     2701 gtagaagaga agtcgcctaa cattgtagga tcataggtga tttgttggac aggtcaagct
     2761 gatatttgag cctgatgtta gaaggaattt ctttttactt gctgtagaaa ttattatatg
     2821 actaaaaagg ctgggatttt aaatcccagc cttttatgac aaatagaatt tttatcgatc
     2881 agtaatcgct gtcgctgcaa attgttcgat caaaggaatc aaagtcaatt tgactttgta
     2941 tttccgttcg agtgcttttt cgataacgaa ctcattttca gtagtcatag aaatagttgc
     3001 taatttacga ggttcatcag catttttttc attaataact acttcatgtt cattttgttc
     3061 tgtgtaaaat ttaggatact cagcaacggc ttttgaaaaa tcagcaaaac ttaaagcggc
     3121 attcgtatca gttgagcgtg tattttcagt tgtgtccgct tttggttttt ccgttttttc
     3181 ttctggcaca ctgactgttt cttctgtaac tggtgtcgca acagcttgcg cagttggttc
     3241 atcgtttttc aatcgttgat cattccagta agacacgatt tcaataagaa gattgtcacg
     3301 gaatcgttcg aaaatcccgt tttctcctga acgaattctc tttaatacag tattgaagtt
     3361 gtcatcgatt ggcacgttga tccgtgtatc ttcatttgtt ttcccagcta atccgatcgt
     3421 taagatttga cggtttttgg gatcaagcat gacttgtaac ggcaaaccat aacgtcgcaa
     3481 gctctcttct tctagagaac ggaataattg tcctcttaca ccatccaatg aaggacggaa
     3541 ttgatttaat aatttttgtt tctgtttttt cttcatatgt tctctccttc gctcataccc
     3601 tttcgattat aagaaaaaaa cagccaaatg aaaaggagta atgcaaaaaa acttgcgtat
     3661 acgcaaatta caacagaaaa atttgcgtat tttcccgttt tttgaaagaa aaataaaaaa
     3721 tggaacggat tatgtatata tacataatcc gttccattct ttctattttt tcggtatcaa
     3781 ggcaaaaaca ggtagttcag gtgaataaat gacttcatgt tcgtgccagg gtttgaccat
     3841 tagcgaatga tcgaaccgtt ttttcagttc ttcgatgatt tgatcatgtg tccagtttct
     3901 tgcaggaaaa tgttggattt tctctgcatc gatcaaaaag acaaaatcgg aatgaaaagt
     3961 ctgggtaact tcttccgaga gagaattcaa gcgttcgatt ggatccataa aatcaccttt
     4021 cgacttaaaa ttgggtagta tctgggtctt ttgctttgcc gactacacca tctaactgat
     4081 cgatttgttt catttcttct tctgttaatt caaaatcaaa taattcagta ttttctttga
     4141 ttctgcttgg tgtcactgac ttaggaagag gaaggaattc gtgttgcagc gaccaacgca
     4201 aagcaacttg tgcaatcgtc ttgtcatgtg cttcagcgat ttgtttcatt tcaggcacat
     4261 cgaagatttt accagtgcct aatggactat atgcttcgag gatcatatca tgtttttttg
     4321 catactcaac tacagttgtc tgtaattcgc ctggtgcaag gaaaacttga ttgaccatcg
     4381 gcataatttt tgctgttttt gctagtgtat ctagatggtg gggtaagaaa ttgctcacac
     4441 cgattgcttt gattttacct gcttcataca gttcttcaaa tgctcgccat gtatctgcat
     4501 ttgcttgttc ccaattatca cgaaaagcaa cagggtttgg ccagtggatc aaaaacagat
     4561 ctaaataatc tgtttggagc ttcttcaatg attcttcaaa agaagacatc acaagttcat
     4621 agctgtggtt tgcattccat aattttgttg tcaagaatat ctcttctcta ggaattccac
     4681 tttccttaat cgcttggccg acgctttctt cattcttgta tccttgggca gtatcgatat
     4741 ggcgataacc tgcagccagc gcttctttaa cagaggaaac agcgacatcc ccatcaggtg
     4801 tttgccatgt gccgaatcct actaccggga tttcgtaacc atttgataga cgataagtgc
     4861 ttgttaatga attcatactt tcagctcctt ttttattcgt tcaactataa gtgtagcgca
     4921 tttttcgaaa aaaagaatcc attttgtttc tactcttgaa atttttgttt aaaagaaaat
     4981 gattttgatt ctggtatttt tgattttttt tccgtaaact aagacagaag ggaatgagaa
     5041 aatggatatc tatttaaaaa aagcaattct gcatattatt gacagagaaa ctggttctcc
     5101 agtcttttca caaaaagagc tggatctgac aaaagaatat attcgtgatt ttcttcaaaa
     5161 gaagatccaa aaaatctctt ctgctcaaac aaaaactgga caactagcag aagaaagcat
     5221 gatcgttgaa tcacttaaaa tcgctcaaga aaattttgtg gaagcaagtg aagcaatcgt
     5281 tcagcggtgg ttcgatatct accaagaaag tgaagaagca cctagtgcag atgtatttgt
     5341 tgtcttgtat gaattagata cagtgatgta cttatcttta ttgaaagtca attatcgtga
     5401 ggcttacact cactttgttg aagcagacga agcagggatc gacaaccgct tgattttgaa
     5461 tcgagcaatt ctaggggcga aaacacaaaa agcagatgaa gcattcgctg tgaatttgac
     5521 tgattggacc tacgaattaa ttgaaaaaaa atatgagttt tctggcaaaa aagagcttta
     5581 tttttctgga agagtgatcg aaagtgttcc tgcaccttct ttagaagaaa atgtaaaagt
     5641 gatccaaaaa gtagccaagc atcttggaaa aaaatttgag acagaagaat ttgatatcat
     5701 ggctgatttg aaagaagctg tctatgatac gatagaagaa aaaggtcggt tggatcatga
     5761 gatgattgcg gaaaaagtct tcaaggaaaa catcacggca aaaatggctt ttaaagaaga
     5821 agtacaagag caaggatttg tcccagaagc acctcctgta aaagaagtac aagaaatttc
     5881 tgagaaaaaa tttggaaaac aaaaattcaa gctatcaaac gggatcgaac tgatcgtacc
     5941 agtagatgta tatcgcaacc cagatttgat tgaatttgtg aataatccgg atggtacgat
     6001 atccgtgatg atcaaaaatg tggatgaagt attgaatcgt ttataaaaat gactagaata
     6061 ttaggtgttc aagtaccagt gatcagttaa atgaattgtg tcaagagaag gatcggaaaa
     6121 gacgaatata acaaaaaaag agcgtgagac aaaagtaaag aacacttttg tctcacgctc
     6181 taaacacgta taaacggcgg aagcagaagc aactccttct tatttcttgg agttaaacgc
     6241 ttctgtccca gcctcttttt atctaataaa attttaagac catttactaa ttatcgattt
     6301 tttctgccgc atttttaggc acttcagaag cctttatttc ttcccagctt aatacacctt
     6361 tacgaggatt gactttgagt ttaagatacg cattcatctt taatggatgt tctctttctt
     6421 cgcgaagcgt cacctgtttt ttttcaccat ctttattata agccgcttgc ttgtaggtat
     6481 aagtcgtgaa tttctctccg ttatctgctt gggtagctga catttcgcca ttcgtcgtga
     6541 tttttgtata ataagcttcg ccgccgtaaa aataatgata agctgcaaaa cttccgccga
     6601 gaaatatgcc taaaacgaca agtacgccaa ttaatttttt catgtacttc acctctttcc
     6661 ttattattaa cgaaatagaa gatttcgtct attgaaagtt gttggcaaca gctgaatttt
     6721 aaacttttcc tcattttgtt cttattgctt caaaagaaag ttaaggacta tcttgaagtt
     6781 tttctcaaaa aacacatttt tttatttata aaaggtactt ttttcgcgta taagacagac
     6841 gagtgctctt agttgacttt gaccaatatt gacgtataat aagcttagtt cgaatcttac
     6901 aaaaagtaag tcggacgtat tctttttgat ttagtcaaaa aaagactata ataaaaaggt
     6961 gagagctgaa atttttcagt gatctatatt atttaggagg tttgtaaatt atggcaaaag
     7021 aacattatga cagaagtaaa ccacacgtta acattggtac gattggacac gttgaccatg
     7081 ggaaaactac tttaactgct gcgatcacta cagtattagg caaaaaaggc ctagcaaatc
     7141 ctcaagacta tgctagtatt gatgcagctc cagaagaacg tgaacgtggg attacgatca
     7201 ataccgcaca cgtagaatat gaaacagaaa aacgccacta cgcgcatatt gatgcaccag
     7261 gacacgcgga ctatgtgaaa aacatgatta caggggcagc acaaatggat ggtgcaatct
     7321 tagttgtttc tgcaactgac ggtccgatgc ctcaaacacg tgaacacatt ttattgtcac
     7381 gccaagttgg tgtaaaatac ctgattgttt tcttgaacaa agttgattta gtcgatgatg
     7441 aagaattgat cgatttggta gaaatggaag ttcgcgagtt attgagcgaa tatggtttcc
     7501 caggcgatga cactcctgtg atcaaaggtt ccgcattaaa agcattgcaa ggcgatccag
     7561 atgctgaagc tgctattatg gaattgatgg atacagtaga tgaatatatc ccaacaccag
     7621 aacgtgatac agataaacca ttactattgc cagtggaaga cgtcttctca attacaggtc
     7681 gaggaactgt tgcctcaggt cgtattgatc gtggtgctgt tcgtgtcggt gatgaggtag
     7741 agatcgtagg gatcaaacct gaaacacaaa aagcagttgt aacaggtgta gaaatgttcc
     7801 gtaaaacgtt agattacggg gaagctgggg ataacgtagg cgtgttgtta cgggggatcc
     7861 aacgtgacga tatcgaacgt ggacaagtac ttgctaaacc aggttccatt actccacata
     7921 caaaattcaa agcagaagtg tacgtgttga caaaagaaga aggtggacgt catactccat
     7981 tcttcaacaa ctatcgtcca cagttctact tccgcacaac tgatgttaca ggaacaatca
     8041 cattgccaga agatacagaa atggtcatgc ctggtgacaa cgtgacgatt gatgttgact
     8101 tgatccatcc aatcgctgtt gaaaacggaa cgacattctc aattcgtgaa ggcggacgga
     8161 cagtaggttc aggtatcgtt acagagatcg aagcataaga aacgcgactc gaatattttg
     8221 gtaatgaaaa atgtttaaaa taaaagagcg gtccaaacag gatttctgtt tggaccgctc
     8281 tttgttcttc aattgtataa gaagagattg tagaagcatg aactttcgtt ttacttcagt
     8341 ctcttttatt catcatgaga gtgttcatct atcggtatgc agtttttaat actttcttcg
     8401 tatccatttt ttcaaaccac ttattctacc ttgtgtctgc tcaactggcg cattatcaat
     8461 aatcagcaaa gcaatcataa catatgcaat ccaactatct ctagaatata gagtataacg
     8521 tgagacccag tttgtagcgt acttgctttt atattctctt aatccatgga aagaataaaa
     8581 atgagaacca aactcgtaaa ccaagtatgc aatcctttcc tgtaaaaaac tctttctgga
     8641 agttccgaca tttgaaagag gggccatccc taaattgaac cattggatat gttcttcttt
     8701 catataggca aacaaatgca agaacaagaa atccatactg ccagaaggtg cttttttaga
     8761 atcatatcgc atcagatcaa tcgttcctac tttattgttc gtgtaagtag gcatgatcgt
     8821 tgcaaaagcg acaatttctt cttgggtatt tttgacaact gcaataggcg ctctttgtag
     8881 ataatcttca gagaaaaagc ctaaagaaaa ccctttttcc tttcgtgaac ccaaccaatt
     8941 atcagagata tttttgaaag tactcatttg ctcagtagaa aatggtggtt gtaagacatc
     9001 aaatgtaaat ccttctcgtt cgatccgatt caagacagca cgagtgccct tcattttttt
     9061 ccctgaagtt gtaaaactat tcaggtctac ataagcttct tcccccatct taatgaaatc
     9121 gtagccgaat tcatgcaaaa tcataacaat ctcttcgctt gtttcgtaga aaactggtaa
     9181 atagcatagt cgatctgttt cttcgataaa agcttcgatt gcttctggaa aatcttcttt
     9241 cttaccagaa ggatcaccca ttacaataca tttattattg aagcaagcga attggagaag
     9301 aacagttggt tcaccgtctt tttcataggc aaacatccgc ttatctttta agaagatcaa
     9361 ttgactatcg gagtttcctc cataagttgt aaggatcttc agggcctttt cttcattaaa
     9421 ggcttcgcca atctgtttct tttctccttg aaggaaatga acaaatagaa cgatcatgaa
     9481 actaacagcg atgatggcca acaatcccga aaaccaaatc ttttcagaag gaaataagaa
     9541 aaaggaaata aaatggtgac gccgatgagg gaaatccggc agattgtata ccccaataac
     9601 gatgtaaagc aaggacagtg caccaataat gattccatcg atcgtacgcc actcccatga
     9661 atagatcaat tgttcccgaa ataattcgtt tttagaagca agaatgatca gaagcagaat
     9721 agacaagaaa accaccggtg tgaaactaaa atcacttaat aaaacataaa gtaaagctag
     9781 aacaatcaag aagattgtag gcaaatatgc tcgtttcaca cgtgctgcga tccctctgcc
     9841 cataatgatt aaaagaaatc ccaatagtat actaggaaat tggataataa aatgaaattt
     9901 caatgggttc aaactatgaa gccagcgaaa ctctgtaaaa gcttgcggga tagtggcaga
     9961 aagtactagc atgattccag aaaagtataa taagacaacc acgattttat gcgcaatttc
    10021 tgttgctaat tgtttaggga taccagaata acgctgatca aaagtggaac cgatattttt
    10081 gaagaaaaag acgattccaa tcaaaaacgg aatgatgtag tagaacaggc gatagagcaa
    10141 gatccacaca acgactattt ctcttggcac accaatagcg gacaacccga taatcatcat
    10201 tacatcgaaa ctcccaattt ctcctggaat catagacacg attcctatca ctgaagcagc
    10261 aatgaataat ggaattgttt gccacagatc aatccggatt tccattaaat aaccaacaga
    10321 aaggaaacta aacaggacac cggtccattc caacaatgaa actagcaata gctgtccttg
    10381 agttttaagt gaggtaccac caaggaatcc tttctttttg aaagtagtaa ataaaaagac
    10441 gacaggaaaa tatagaccgc cgccgatcaa ccatatccaa tattgcttca aaaaaggagc
    10501 aactggcgtg aagactacaa gtaaaaaaga aagcagactg taaatagaaa gtccagccat
    10561 caaaaacaag aaaactttcg ataatgcttg gatcacgttt ctgctctctt tcttttgtcc
    10621 atatagttca gagcgaagcc ccacactaac aaacccgccg aaaccggcaa tattgttgat
    10681 cgtattgatc agccagcttg tctcaaacag gtaacgagga ttctgctttt gttcaagcat
    10741 ccgattcaaa ataatatcat aaccaaccat aggtaacaca gagataagac caatcaatag
    10801 cattagtccg cttttccata caggtatagt ggcaaacgtt tctcctaatt gctggataga
    10861 tagagtttta ccgatggaca tcaattcacc gacaataatg acaatgacag aaatcaaaaa
    10921 gattgttttg aacagtccta ggtggttttt cagccattgt atggtatttt ttaacaaata
    10981 aatcaccttc tattcctaat agttatgttt cagaaagaaa ttgaagaact tctcgattga
    11041 agcgttcagg atcttttcta gctaattcat gtccttgttc tttcacaagc acaaaggaag
    11101 cttttggtat agttttagct atatacaaag aatgttttag cttgattaca tctttttttc
    11161 ccacgatgat caaagtgggg gagttgattt tttttaaatc attttctgta agcccgatgt
    11221 catgcagcaa aagtttgata actaaaagat ttttgcgaag acttgggcga aataaagaga
    11281 gtaaccatac ccatgcataa tgaatattac tgatgactct ggcagaaaaa cgaacgcctt
    11341 taacaagcgt gtttccagaa tttaaaatca atcgatgaac ttttttaggg aagctgctgg
    11401 caaataccag tgctagattt gctccatcac taaatccaag aaaatctgcc tgatcgattt
    11461 tttccagcaa catgatcgta ttcagatcct cggccattaa ctgaaaattc aacatagatg
    11521 cttcgtttgt cgaacgacca tgtcctcggc tatctactag atacacggta tagtagcgtt
    11581 cgagtacagg gacctgttct gaaaaaaagc ggccgctgcc gtcattgcca tgcagtaaaa
    11641 agagagggaa gccttgaccg ctcttttcat agtaaatttc gctcccatct ggcatttttg
    11701 catatttctt ttcttttttc acataatcac ctactgtgta aagtataacg attatatcag
    11761 tcattgggca attgttttta ctgaaaaaca acgatcattt tcgtaagatg tcaaaaaata
    11821 caggaaaatt cattttttct taaggcgtac acagaaatca aggaagaaat agccaagaag
    11881 ataaagaaat agtaagataa aaaaagatgt caaaataact aggtgatgaa atgaagcgaa
    11941 aacgattatc tattatccgt attctttttt acatgatcat tctgacggtt attggtgcgg
    12001 tgttgttttt atgaggaaga acttatcgtt cggtaaaacg agtggaaagc tatcaaatag
    12061 aaataacaac agctgcaaaa aattatcata tggaaaacta cgagaatttg atcaaggcga
    12121 taatattgac cgaatcaaaa ggaaaaggga cagatctcat gcagagctcg gaaagtctgc
    12181 atggagaagc tgagatcatt gataatccag ctgacagtat tgatcaaggt gtcaaatatt
    12241 tggcggaaat gatagtagaa gcaaagaaac aaggatgcga tttatggaca gctgtccaag
    12301 catataattt tgggaaagat tatattgcct atgtaaaaaa tcatggcgga gaaaacacag
    12361 tagaattagc agaagcgtat tctaaagatg tgctgagccc acttttagga aatgaggaag
    12421 ggaaacaata ccgatattgg cggatccagt ccttgcttta caatggcggc tatctctatc
    12481 ataatggagg taacatgttt tatcctgata ttgtcaaaat gaatgaaaaa ataatagaga
    12541 cctatgaaaa agtctttaag taaaatagca ggaaatgttc aatcatgaat aaaattaaat
    12601 gaaagaagga gtgcgcaaat agtggaaaaa aggcaaaaaa tggaaaatca agaagtgggg
    12661 tacacaacca aagactggat gttacggttt gtgaagggaa tgtttatcgg ttcaggattt
    12721 atcttgccag gagtcagcgg tggtgcatta gcagctgtct ttggcatgta tgagcggctg
    12781 atttcttttt tagcccacct tacaaaaaat ttcaaagaaa atgttttgtt ttttctgcct
    12841 gttggtctag gcggagtgac aggcatcttt ctactttctt ttttagtcag ttttttatta
    12901 ggtgcgtatg aaacaacgat attatggttt tttgtcggat gtatcgctgg aactgttcct
    12961 gcgttatgga aagaagcagg gaaaaaagga agagatactt ctgatgtgat catcatggtt
    13021 gttacttttg ttctggctta tttgttttta ttatacggcg ctcaattatt tgacgggcaa
    13081 gtcgatcaga attttggaac atggctgatt gccggcggat tgattggttt gggtatgatc
    13141 gtcccaggat tgagcccttc taattttttg gtgtatatgg gattgtataa agcgatggct
    13201 gacggattta aagaagtacg acttgaagtg attgtgccga tcggaattgg tgggattctt
    13261 tgtgttcttg gattgtcaaa aatcatggat tacattttct caaaggcata tagcaagcta
    13321 ttccatttca ttttaggtgt agtttttgct tcaacgatta tgatcattcc taccgattac
    13381 gcgggattgg gagttgctgg ggtactagct tgtcctgttc tttttatagc cggtacagca
    13441 ctaggttggt ggatgagccg tttggaggaa caatataagt aaagatagat tgataataaa
    13501 aacagcacac gggtgattca aacaaaaagg ttggcatctt ttttgtaaaa tcattcgtgt
    13561 gtttttctat aaattttata aaaacaagtt gtttttaatc ttggaacacg ctagacttaa
    13621 acacgtgatt atttttgaaa gttggcggaa aatggagaaa aagaaaatga atggacttac
    13681 acagcaagag gtagttcaga aaatggcttc tggccagcaa aatgactacc aggaagatgc
    13741 atcaaaaagt acaaaacaaa tattcagcga taatatattg acgttattca attttctgaa
    13801 ctttggtatt gggatttgtc tgttatttgt aggtgcgtat tccaacatgg cgtatttagc
    13861 aattattgtc gtcaatatcg ttattggaat ttatcaagaa atacatgcaa gaaatcttgt
    13921 acggaaatta tcgattgttt cccaatcaga agtactagtg atccgggacg gtaaagaaca
    13981 atcgatttct actaaagagc tggtattagg agatttggta aaaatcggag caggagaaca
    14041 gataccagcc gatttacagg ttatatctgg acgggcagaa gcaaatgaag ctcttttgac
    14101 aggcgaatct gatttgattg tcaaagaaac tggagctgaa ttgctttctg gaagcttttt
    14161 gacaagcggt tctgtcatgg ctgaagttat ccgagtaggt gcagataatt atgctgtccg
    14221 cttgactagc gaggcaaaga ctcacaaagc tattcgttct gaacttgtag attcgattcg
    14281 caaagtatcc aaattcacta gtttcgtgat tattcctttg gggatcatac tatttttgga
    14341 agcactgttc atccgcgatg cttctatcaa aatttctgtt attgcatctg cagcagcttt
    14401 gttaggcatg cttccaaagg gtttggtatt gctaataagt attgcattaa cgacaggggt
    14461 aaccaaactg gcaaaaaaac gaatccttgt tcaagacatg tattcaattg aaacgctggc
    14521 ccatgtcgat acattatgct tggataaaac agggaccatt actgaaggga aaatgagtat
    14581 ccagcgggta gaagcactac atccgctgta cgagaaagcc atcccagaaa ttatggggac
    14641 gtatctggca gaaagcagtg ataacaacat cactatgcgg gctatgagag attattttga
    14701 gctttctaat cggtatgatg tgacaaacaa agtcgcattt tcttctgatc gcaaatgggg
    14761 cgcgatggaa tttccagaac tgggagtcgt gtacgtagga gcgcctgaac gtttggtgga
    14821 agaagatcgg cttccggaca gtctaaaaac agcccaagcg aatggctatc gcgtacttat
    14881 gttggggatc gctccaaatc agcaattgaa cgaagaaaaa ccagcatttg ttgagccggt
    14941 tgctctgttt gaaatcgatg atccaatccg aaaaaacgct caagaaacac tagcttatct
    15001 tcatgaggaa ggtgtggatc tgaaagtgat ttctggagac aatcctttta ctgtctccaa
    15061 tatcgctaga cgtgcaggat taccgaatta tgatgcgtat gtcgatcttt ctcaaattac
    15121 tgaagaaatg gaagtgcgag aagctgctca tcgctatacg gtttttggac gagtgagtcc
    15181 gcagcaaaag aaattgctgg taaatgaatt gaaagaaagc ggcagaacgg ttgcaatgac
    15241 aggcgacggt gtgaatgatg ttttggcgtt aagagaagca gattgcagca ttgctatggc
    15301 agaaggtgat ggagcaacac gtcagattgc gaatctggta ttgttggact ctgattttac
    15361 tacattgcca gaagtcctgt ttgaaggacg ccgagtggtc aacaatgtga cgaaagtctc
    15421 aggcatcttt tttatcaaaa cgatttattc atttatttta tcgattatat gtgccgttac
    15481 agcaattgga tttccgttta tccctatcca aatcacgctg attgatttag cgattgaagg
    15541 gtatccttcc ttctttttat cttttgaaca agataaaaga aaaatcactt accgttattt
    15601 gcctacagca cttattaatg ctttaccaaa tgccttgctt gtagtagtga atatcattgc
    15661 ggtgtatcta cttggaaaaa cattcgcatg gggaaatgct gaaactacta ctttgatgta
    15721 ttacttgctg attgggatca gttgcatggc agttgtaaaa gcttgttatc ccttcaaccc
    15781 attgcgcatc ttcctagcag taacgacgat cgtagggata tatgtagctg caatgctgtt
    15841 ccatggaatt ttggaagtaa acttagggtt gggagagagc gtaggatact tctttgtatt
    15901 catgctcgta aatattgttc ttcgagtatt gtactggaag gctgatattc aaaatcagtt
    15961 actgaaaaga gtagcaaatt gatgcttaat gagatagaaa tgtgttatac ttgaaaaagc
    16021 aatgaaatat gatacaatca aacatgtata attgtgtcct ttaaaatcgc actgatttca
    16081 gttagtgcga ttttttaccg gttattctcc tgaaatatct tctgttaaac ccaaaagata
    16141 tcaatagagc agggggtgaa aaaatggctc aacggattcc tcaagaggtt atcgaaacag
    16201 tacgcagtca aacgaatatc gttgaggtga ttggacaata tgtccagttg aaaaagtctg
    16261 gtaaaaatta cttggggctg tgtccttttc atgaagaacg tacaccgtct ttttctgttg
    16321 cagaagacaa gcagattttt cattgctttg gttgtggtaa aggcggaaac gtgtttactt
    16381 ttcttcaaga actagaaggt ctaagttttc cagaagcagt ggtaaaagtg gcagacttcg
    16441 aacagatacc gttagaagat caatggcgtc agcgtactat agctgtccaa aatcctgaat
    16501 ctgctacggg gaaactgatt gctgctcatg aaaaagcagc ggaaatctat catcacatgc
    16561 tattgcatac aaaagctgga cagccagctt tagaatattt gcaaagtcgt gggttgtctt
    16621 tagaattaat tgaagaattc aacataggtt ttgcacctaa tgagcgttct tttcttcagc
    16681 aagtattcaa gaatgaatca atttcaaatg aattgttaga acgctctgga ttgtttgtca
    16741 cgcatgatga tggccgtatg tctgatcgtt tttatcaaag agtgatgttt ccacttcgta
    16801 attctcaagg ccagacgatc ggtttttcag gacgctggct tgcacctgaa aaagggcagg
    16861 aaaaagatca gcccaaatac ttgaatagtc cagaaacaga actgttcaat aaaagacaag
    16921 ttttatttaa tcttgataaa gcaagaagcg atatccggaa aaacggagaa attttgttgt
    16981 tcgaagggtt tatggatgtg attgctgcat ggcaggctgg tatcaaaact ggattagctt
    17041 ctatggggac aagtctaaca gctcaacaga tccaacaaat ggaaaagctg acgaaagaat
    17101 tggtcttttg ctatgacgga gacaacgcag gttttgaagc aaccaatcga gggattgaat
    17161 tgcttcggca aaacagtcga ttagcacttt ctgtcgtagt cgttccagaa aagcttgatc
    17221 ctgatgaata tctccgtaag tatggagaag catctttcca agaattagtg gctcatgggc
    17281 gcgaaacagt ctttactttt aaatcacgtt atttaaaaca agggaaaaat ttagataatg
    17341 aaaaagacaa gatcgaatac ctcgaagaat tactagttga acttagccga gttgtttcac
    17401 caatcgaaca agatatgtat ctatctcaac tgtcaacaga gtttcagatc ggcagagaga
    17461 cgatacaaaa acaactgcgc gatcttcgaa ggacaaatca gcaaaatcgt caagaacctc
    17521 aggaaagtca acacactcag gttacgagac aagaaactgc acggaaaaag agacctttga
    17581 cgcaagtaga aaaggcagag cagattctat tatatcgtgc atttaaagaa gtttctgtcc
    17641 gtaatatttt aaaagaacga gaatttcagt ttattcatga tgcatatgca gaagtttatt
    17701 ttttattcga tgcatatgtc tcacaacatt atgaattcaa ccttgcagag tttttagact
    17761 ttttgcaaga tgaaaattta aggcggttgg tggtggaaat cgaatattta ccggtggcag
    17821 aagaaagttc gccacgagaa atccaagact tgctagatgt gatcagcaaa tctggcttag
    17881 ctgatgaaat aacattaaaa aaacaaatgc agcaagaagc cagacgaact ggaaacaaac
    17941 agcttgagct tgaattgacg attgaaatca tcaatttaac gaaacagtta aaacaagcca
    18001 aatagctact caaggggggc cttcttttat ggcaaatgga acagataaaa aaacgtatga
    18061 agcagcagta gcagctttca tcaaagaaaa caaacccaaa cgacaagttg tatatgatga
    18121 tttatccaat cagcttgcta caccattcac tttgaatgca gatgaaatgg acaaattgat
    18181 ccaaaagatt gaagacgcag gtatcagtgt ggtggatgaa aatggtgatc catcaattca
    18241 cagcttgaag agtgctgaga aaaaagctga acaagcaaaa acagaagatc tttctgcgcc
    18301 aaccggtgta aaaatcaatg acccggttcg tatgtatctg aaagaaattg gccgtgtctc
    18361 ccttttaact gcggaagaag aagtagcgtt agcattgaaa atcgaagaag gcgaccaaga
    18421 agcgaaacag cgtctggcag aagcaaacct tcgtttagta gtaagtatcg ctaaacgtta
    18481 tgtaggacga ggcatgcagt tccttgactt gatccaagaa ggaaacatgg gactgatgaa
    18541 ggcagttgaa aaatttgact accgcaaagg atttaagttt tcaacctatg ctacatggtg
    18601 gattcgtcaa gccatcactc gagcaatcgc tgaccaagca cgtacgatcc gtattccagt
    18661 tcatatggtt gaaacaatta ataaattgat acgtatccaa cgtcaattgc tgcaagacct
    18721 tggaagagaa cctacgccag aagaaatcgg tgcggaaatg gatcttccaa cagaaaaagt
    18781 ccgagaaatc ttgaaaatcg cgcaagaacc tgtttccctt gaaacgccaa tcggtgaaga
    18841 agatgactct cacttaggtg atttcataga agaccaggaa gcaacaagtc cagcagaaca
    18901 cgcagcttat gagttactaa aagaacaatt ggaagatgta ctagatacat tgactgatcg
    18961 ggaagagaat gtccttcgct tacgttttgg tttagatgat ggccggacga gaacattgga
    19021 agaagtaggg aaagtatttg gtgttactcg ggaacgtatt cgtcagatcg aggctaaagc
    19081 attaagaaaa ttgcgccatc cttctcgttc aaaacaatta aaagatttct tagaataagc
    19141 gtggactatt gaccagaaga gaaatcagta aaactgtttg gtttatttta aaagaaaaaa
    19201 agcttgagac tacgcagtct caagcttttt tttctatagg aaggaaattc attcattatt
    19261 tttaagtaaa tcctaaaaat aagctattgg cgtgacgtat agaatatgat agattaaatg
    19321 gtagcgtaaa cctagtaaag gagccgatcg ctaagtgatg aaaaaatgtc caaattgcgg
    19381 atcgaaaaaa atggagcaga atactatctg tccagactgc ggtcatcaaa taactgcaga
    19441 agatcagcaa ataaaagaat caattgaaga aacaaataca gatgcagcta tgcaggataa
    19501 atcaactgaa gatgcagtct ttcctgatac agaattaaac gatccaatcg aatggtcgga
    19561 attgaaagat ttacctctag aatctgtaat ggaattattc gaaaaagcag aaccagaaga
    19621 cgaagaacca cctgctcacg atcaagtaac taatatagag aaaaatgaac agaagaaagc
    19681 aactgttcat gagacttcgg aagaatcagg aaagaatcaa gaagaaacgc ttgaatcaaa
    19741 acaaaaaaga agagtagctg aactgaaaga aatcgtcgat aatgaggaag aaaattcgat
    19801 actatccgct tatatcaaag cacatcgtga agatacaaaa gaagagcacg cgaaagagct
    19861 gctcaaaatg atcagtgaaa aaattgcaca ggaaaacaaa gtagagccag aaaaggaaca
    19921 agaaactgaa aaagactctg atagagaaaa cgccacaggt aaagaaacag taaaaataaa
    19981 agacgtaaaa acggatatca cagaaaaaac tgaagtattt actgaaaaac aattagaaaa
    20041 ggaaaaaacg aataaactgc cggaagcaga gaaaaaagaa atactcgaaa acacagacaa
    20101 aaagtcaaca gagcccgatg aaataaatag tgaatccgat gctgaaaatc aatctgtaag
    20161 caaagagggt actgatcttg aagcaaaaat agaaaaagaa gagaaccagc ctaagctgtt
    20221 tgaagcagaa gcgaaatcaa taacaatcgt gccagatact gaaaaaacac atcaagaatc
    20281 aaacttagaa aaaacagatc cagataaagc gcaaaagaaa aaatcaaaaa aagtacctta
    20341 tctcgtttta gcagcagtac ttctactcgg tggaggaagc tgggcttatt atgaccatca
    20401 tcaaaaagta gaagccgaag tagcagcaga agcaaaaaga cagcaacaaa tggaagaact
    20461 tcatgagaaa cttgctgcct tttatacaga tgatcaccat caatttattc gtgcagatat
    20521 gattcatcaa gatttatcaa aactaaaaaa tgatttatcc agtcttaaga atgaaaaagg
    20581 atattcagag ttagaaacaa cttaccaaga tatccaatct aagatccgag atatccaaaa
    20641 agtgaatgag tggttcatag cacctgccat cgtcgatgat catctagcag atgacatcaa
    20701 gttgaaagca gataaagaga tccaagcaat cgaaacggat aacacagatt ttggaaaatt
    20761 gattcaacaa gcacaaacag aagctaaaaa tcaatatgct gaattgcaaa cagctaaaga
    20821 aaaaacagct atagtcttta aagacggaaa agtagtggat tcagcaacac gcgaccaata
    20881 caagacggca aaagaagcag tagaaaaagt gaaaaataca gatctatctg ctcagttgaa
    20941 tgaacaattg aaacaagtag ataagatgct cactgataaa gagaaaaaag aagaagagga
    21001 aaagaaaaaa gcagaagaag cgaaaaaagc agctgaagaa caagcaaaac aagccgctca
    21061 agcagcagct gcagctcaag ccgctcaaca agctgcggtc gcacaagcag cacagcaaga
    21121 agcggcatcc tcgacccctt cctcttcaaa ctcagcgaat cagccaatca aatctgccag
    21181 agcaagtgat attgccgatg catctaatcc agcatggacg tgggcaccag gtgtccaaag
    21241 caaagtgatt gctacatgta tccaacgtgg atatatcgtt gaaggaggtt acagattaga
    21301 aaaagccaga atcgaaaatg gggaaggcta ttataatcta tatgccactt caacaaaatc
    21361 tgcattgatg aaaggcattg gagaaagtgc tttgccattt tacatcgtaa ccatcaactg
    21421 taaaactgga tggttcggag gaaatgggag caattaattt taggcaaatg aaagaggctg
    21481 tgagcctgcc gtttaacacc gtttattcga aaaaggggag tatgacacaa ctgttgtcat
    21541 actccttttt tcacctttcc aaaccaaggc tcagctattc aagtagataa cattatgtta
    21601 tgatagcaaa gaaatgtatt aagaaaacct ttcactatag agggagaaaa aaatgaacga
    21661 tttattagca actatattta caggtattat tattgatgaa aatgaaaaaa actactttgt
    21721 ccaaaaaaac ggacaaacct ttggcttaag caaatcagaa ggtgaacaca aaatcggtga
    21781 agcagttgaa ggattcggtt accaaaatca aaaacatgaa aatcggatca ctacacaaat
    21841 tcctaaaagc cgtatcggtc attacgcttt tggaatcgtt actgctacac gaaaagattt
    21901 aggtgcgttt gtcgatatcg gtcttccaga taaagacttt gtcgtatctc ttgatgaatt
    21961 gccgacaatg acagagctat ggcctaaaaa aggggatcgc ttgatgattt cccttagagt
    22021 tgatgcaaaa gaccgtattt ggggcagttt agcagatgaa aaaatattca aatctttaag
    22081 caaatatgga acagaagaaa tgaaaaacaa agatgtgata ggaacagcat accgcttgaa
    22141 attaacaggt acgtacgttt taacggaaga tttctatctt gggtttatcc atccttctga
    22201 acgatataat gaacctcgtc taggcgagca gataaaaggt cgagtgattg gtgtccgacc
    22261 agacggtgta ctgaatcttt cattgaagcc aagagcatat gaagctatat cagatgatgc
    22321 acaaatgatt ttaaccattt tagaacgttc tgcagatcag caaatcaact ttacagataa
    22381 atcggatcca gaagaaatca tgaaagcttt tggtatcagc aaaggagcat ttaagcgcgc
    22441 attaggtaat cttttgaaac aaggattgat tacacaagaa aacggtgtga caaaactagt
    22501 caaaaaatct aattgagaat cccttgcatg tttttcttaa ctagcttata ataattttaa
    22561 ttaacatagt gttatatctt tttttatttg taattattat aaattgttgg ggttgagtaa
    22621 atggtcatga atgctatttc ggcgatgaaa aaaacaaaac agcagctaca tgaagcgggg
    22681 tttaagctga caccgcaaag agaagctact ctgcttgtgc ttttggaaaa tgagaaagag
    22741 catttatctg cagaagaaat cttcttttta gtgaaaaaaa agagtccgga aattggactt
    22801 gctactgttt atcgaaccct tgaaatcttg acagaattga aaattgtcga taaagtaagt
    22861 tttaatgatg gattagcccg ttatgatttg agaaaagaag gcgccaaaca tttccatcat
    22921 catttactat gcttgaactg cggcacgatc gaggaagtag aagaggatct tttacttgaa
    22981 gtggagcaag tagtagaaga acgttatcat tttttagtaa aagatcatcg gctaacgttc
    23041 catggaatct gtcaggaatg ctatgaaaaa gaaaa
//
LOCUS       DACDXE010000045        23066 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.045, whole genome shotgun sequence.
ACCESSION   DACDXE010000045 DACDXE010000000
VERSION     DACDXE010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.045"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(109..564)
                     /locus_tag="IWM23_001739"
     CDS             complement(109..564)
                     /locus_tag="IWM23_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884373.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(718..1926)
                     /locus_tag="IWM23_001740"
     CDS             complement(718..1926)
                     /locus_tag="IWM23_001740"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAP9884374.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2038..2370)
                     /locus_tag="IWM23_001741"
     CDS             complement(2038..2370)
                     /locus_tag="IWM23_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9884375.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2518..3393
                     /locus_tag="IWM23_001742"
     CDS             2518..3393
                     /locus_tag="IWM23_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9884376.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSGELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3475..4056)
                     /locus_tag="IWM23_001743"
     CDS             complement(3475..4056)
                     /locus_tag="IWM23_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAP9884377.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4062..5321)
                     /locus_tag="IWM23_001744"
     CDS             complement(4062..5321)
                     /locus_tag="IWM23_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAP9884378.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5323..6369)
                     /locus_tag="IWM23_001745"
     CDS             complement(5323..6369)
                     /locus_tag="IWM23_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884379.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6604..6879)
                     /locus_tag="IWM23_001746"
     CDS             complement(6604..6879)
                     /locus_tag="IWM23_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAP9884380.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7122..8432)
                     /locus_tag="IWM23_001747"
     CDS             complement(7122..8432)
                     /locus_tag="IWM23_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAP9884381.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWM23_001748"
     CDS             complement(8614..9843)
                     /gene="rpsA"
                     /locus_tag="IWM23_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAP9884382.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9978..10658)
                     /locus_tag="IWM23_001749"
     CDS             complement(9978..10658)
                     /locus_tag="IWM23_001749"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAP9884383.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10707..11342)
                     /locus_tag="IWM23_001750"
     CDS             complement(10707..11342)
                     /locus_tag="IWM23_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9884384.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11408..12811)
                     /locus_tag="IWM23_001751"
     CDS             complement(11408..12811)
                     /locus_tag="IWM23_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAP9884385.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12798..13829)
                     /locus_tag="IWM23_001752"
     CDS             complement(12798..13829)
                     /locus_tag="IWM23_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884386.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13860..14081
                     /locus_tag="IWM23_001753"
     CDS             13860..14081
                     /locus_tag="IWM23_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAP9884387.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14246..14983)
                     /locus_tag="IWM23_001754"
     CDS             complement(14246..14983)
                     /locus_tag="IWM23_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAP9884388.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15136..16476)
                     /locus_tag="IWM23_001755"
     CDS             complement(15136..16476)
                     /locus_tag="IWM23_001755"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAP9884389.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16713..18236)
                     /locus_tag="IWM23_001756"
     CDS             complement(16713..18236)
                     /locus_tag="IWM23_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAP9884390.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18614..19225)
                     /locus_tag="IWM23_001757"
     CDS             complement(18614..19225)
                     /locus_tag="IWM23_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9884391.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19348..19473)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19555..20271)
                     /locus_tag="IWM23_001758"
     CDS             complement(19555..20271)
                     /locus_tag="IWM23_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9884392.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWM23_001759"
     CDS             complement(20277..20846)
                     /gene="scpB"
                     /locus_tag="IWM23_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAP9884393.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20830..21627)
                     /locus_tag="IWM23_001760"
     CDS             complement(20830..21627)
                     /locus_tag="IWM23_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAP9884394.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21597..21968)
                     /locus_tag="IWM23_001761"
     CDS             complement(21597..21968)
                     /locus_tag="IWM23_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9884395.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWM23_001762"
     CDS             complement(21987..22874)
                     /gene="xerD"
                     /locus_tag="IWM23_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAP9884396.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6468 a         4926 c         3911 g         7761 t
ORIGIN      
        1 aaatcttgtc acagtccctt attctgaata aacggtgtgg aagagacagc ctatcagtag
       61 taataagaca catctctttc acagcctcat ttttgcgtaa aaatcgattt attcgaaaaa
      121 ttccccgtat acatcttcta cacgttgatc tggattgatc acgatataca agttacctgt
      181 tttttcgctg atttttgttg attgatattg attgtctaat ccttctgttc ctttatctag
      241 atatgggaag tacacaagat caggtgtgcc atttcggtaa aggatttcca ttcgctcgat
      301 atcttcgtat ttcaatgcgc gattaaacat gcccatttcc agtccgccta ggttgatatc
      361 atgtgtcgca actgcgtccc cttcatggaa gatttcgatt ttaaatccag cacacggatg
      421 gatttcaaca aattcactgc cattgattcg tccaaaactt gttgtgattt gtttgatcca
      481 aagatcgccg atatgacggc ggttgatcgt ccaggtttct ccatttctaa aataaaattt
      541 tagcgctgtc atttctttac tcattaaaaa gactccttta tgatgtaatt tattcttgta
      601 ctacctcagt ttagcacaaa tttatgtaag tggtcacatc ttatgttttt tcttctaatg
      661 acgtatctgc ccactttaag tagccaacaa agctgagcag atacagtcta gtgtttttta
      721 aatcaatttt tctttcgata aaaaatccaa aacctgtttt ttatcatttt ttattctttg
      781 ttctacgacg gctttttcag ctaattgcaa agcttcacta acccacttac caggtttttt
      841 tccagtttct tctagcaaga ttttaccatc tactgctaat tcgcttaatg acttgatagg
      901 taaacgatca taggattctc ctacggcatc catctgaggt gtttcgtcaa aatagatcat
      961 caattcttct acttgcagtg catgtttttt gcctaagaag tacaattcga atggttccaa
     1021 agctccagtt tctcgttgtc gtagtccttg gacaagtgct tgaacgtctt taatcatttg
     1081 gttagaacac ttccacgatt tcaaaaagga acgaatctca ttttctttta gatccagttg
     1141 gctgatcaat aatgcccatg cttggctctc ttctttcaat gggacggatg gtaattcagc
     1201 aaaacgtaaa agtgcttcgc cttcttcttt caaaccggga cagcaaatgt agcactctgt
     1261 ttcaataaat gcatggattc ccgcggcccc ttcatttccc aagagcatct tgatgaattc
     1321 cacatttata cgttctacag atattttacc taatagctcg tggttttctt tgatggcgta
     1381 gaaggtttct tcctctaatt tgaaccctag ttgactgaca aaacgcaacc cgcgcatcat
     1441 tcgtaaggca tcttcatgga accgctcatg aggattgcct acagctcgca agatctgttc
     1501 ttctagatct tgcagaccat cgaacaaatc gatgatttct ccatcttctt tcaaagcaaa
     1561 cgcattgatc gtaaaatctc ttcttttcaa atcttctgcc aacgaacgaa cgaattcgac
     1621 atgatctggt ctacggtagt cctgataagt tgattccgtc cgaaatgtgg tgatctcata
     1681 ttgttcatca ttttctaata ccaacacagt tccatgttcg atcccaatat ctatcgtttt
     1741 tggaaaaagc tgtttgattt cttctggaaa tgcgcttgtt gcgatatcca catcatggat
     1801 tggctggttc agcaagatat cacgaacact tccacctaca aaataagctt catatcctgc
     1861 attctctaat tttttcagaa ctggaattgc cttttggtat tccagaggta attgttttaa
     1921 tttcattttt ccaacctttc ctgcgtgttt cttgtcctga gataaagact cagttgcatg
     1981 gtgactgttt gacaagtccg tttcagagtc ttcaaggcca ggaccacttc atcttgttta
     2041 ctcgtcctta cgctcaaagc gattatggtc tctttgattg aatttttcca tgtttttttc
     2101 aaaaacttca gttaagtcga tattcaatga attagccatg atcatcgtaa cgaataaaac
     2161 atcaccaagt tcttctgcta ccgagttaac tggttcagaa gcctttttgc ttttttctcc
     2221 ataagtgtgg ttgacttctc ttgccaattc acctacttct tcagttaatc gagccatctg
     2281 agctaatgga gagaaatacc ccaccttgaa ttgctggata tattcatcca cttcctgctg
     2341 catactgtat aaagaacgct ttttatccat ctttttccct ccaaaactat cttaccaaaa
     2401 ctttaacgca aatcctagat aagagcaatt gagaaaatcg aatttccatg ctacatttag
     2461 gaaggaattt ctaaatggaa attctgtgaa aatacataat tgaaagctgg aattcatatg
     2521 gtcaaacgaa aaacagtaaa agaagttctt tttatcatta taggaacaag tatttatgca
     2581 tttggtcttg tttacttgaa catcgctaat catttagcag aagggggagt ctcagggatc
     2641 accttgatct taagagcttt atttggtatt gatccagctt atacgacttt attaatcaac
     2701 atcccattga ttttactagg aggaaagatc ctcggtaaac gttccttggc ctatactgtt
     2761 ctaggtacac tttctttgtc attcttttta tggatctggc aaagggttcc actagaaatc
     2821 aatttacagc atgatctact gatcgtttct ctacttgcgg gtttagttgc aggtgtagga
     2881 agcgggatcg tctaccgcat gggtggaaca acaggcggca gtgatattat cgctcggatt
     2941 ttagaacaaa attacggcat cactatgggg cgttccctat tggcatttga tatcatcgtc
     3001 ttacttgctt cattaaccta tatcgacttg aaacgtatga tgtatacgtt gattgtttcg
     3061 tatgttttca gtcgtgtgat tgattcgatc ttagatggcg gatattctgc aaaaggtcta
     3121 cttgtcgtct ccaacaaaag cgaagagatt gcaccactac tgatgaccgg tctgcaaaga
     3181 ggtgtgactt tcctttctgg agaaggcgca ttctctggta attcaaaaaa aatcatctat
     3241 atggtcgtta gctctggtga attaactgaa gctaagagga tcgtccacga gattgacgaa
     3301 aaagctttcc tttcaatcat caatgtgcat gaagtggaag ggcaaggctt tacttatttg
     3361 aagccacaga caagattact gaaaaaacta taagaaaaaa gacaaagata ggctatgagg
     3421 aaagattcca catccagctg acctttgtct tttttagtac tatactattt ttaactaatt
     3481 gttatgttca cttacccggt tacttttctt ttgtgtctgc aaaaggatct ttcggttaac
     3541 ttcatcagat aatttcaaaa aagcatccct atctccatct tctagtgctt gatcgatttg
     3601 agcataaagc atttttagtt ttgcctcttc ttctttttct tgaaaatatc gttcaattaa
     3661 ttcagtatct tcttcactga ctgaagaatc ccaagaagaa aatggattgt cttctaagat
     3721 agatagatat tctcttgtct gccaagcatt ttcgaatata cattccacat ataaaggctt
     3781 tttccaattc aaacggattt catgaaaaat ctggtctgtg tccgtaaatt cttttccctg
     3841 caaaaacaat ttcatgggtt ccccttcaac agatagatca ctgatctgca gtcctcgatc
     3901 cgcaaaattc gccccttcaa caaagtggac attgcttaaa atcgcttcat gattggcaag
     3961 ataattcaag atccaaaaaa cttctcttcg gctaaaagat acatgattga ccatccacgt
     4021 taaaaaattc ttcttttccc gtacatcaat tagcattttt ctcacctctc tgttaggtca
     4081 tctaataatg acagtgcctc catatctcct ggttcaagtg ttaaataata agtcaataat
     4141 tcttgcgctc ttttcagctg cccttcttct cttaagaaaa aggcatactc tttcaagaaa
     4201 tctggttcat gtgatagttc atgataagcc tgttcatagt gtacagctgc aacggtaaag
     4261 tcttctaatt cattataagc atgggctagg ttccattctg cataaggatt agcagtttct
     4321 tccatctgat tgatcacttg tatcacatct tcatatcttt catcatccaa gtaaagatta
     4381 cttaaagtaa gtaatgtttc atcttgtttg tctcctactt ctaaagcttt cagtaaaagt
     4441 tcttctgctc gctgtctatc atgcagacgg aaagcatttt ccgaggccag gtgataaagt
     4501 tcgacttggt aaggattttc tttgattcct tcttctaaca ctgtttgtgc ttcttcaatc
     4561 atttcttctt cttgaagagc ttgtcctaga tatagatata acgattgata atgcggatta
     4621 acttctcgaa gttcttccag atagttgatt gcctgctgat tttctttcaa ttgaagattc
     4681 gtaaaagcta gctggaacag tcgatcatct gtcctttctt ctttcagtgc ttcttctaaa
     4741 ggtacgatag ctgcttcgaa ttctccttgc atacttaagg attgtcctaa acgttcctgc
     4801 atcgagattc ctgaaatttc atttatcccg cttgccaaca atgtttcata aaccactgct
     4861 gcctctttga aacgatcaac tgaaaaatac aattcagcta gcgcaaactg gatcaatggt
     4921 tcatcaggaa gcaacccagc tgcttctttc aattttgctt cactcacttc agggattccg
     4981 ataacttggt acaaatcagc tatcgccaat aagctttgcg gatagtaatc actttgctta
     5041 tccacttttt ccaggtattc aaaagctaag tcgatctcat tatcttcgat tgcaatttct
     5101 gctaacggga tgttcagtcc gtcattttct gggtattggt tgatcagttg ttcaaaaatc
     5161 tgttttgctt cgatcaaaaa gccaatagcc agcaattctt cgcctagttc agctaatata
     5221 tctgctgggt cttcttttaa tgctttttct aaaaagattt gtgcttgtga caagtcttca
     5281 tctgataaag cttgcaacat tttttcgcta tttgtattca tttcaattcc ctcttatctc
     5341 actgacgatt tgttgaaata ccgtcattgt tctcaataat tctttttcgt ttggatgttg
     5401 gtatccgtct attataccta ttttttctaa aacaagtatt tttttgcttt tttctgccaa
     5461 aaaaagaact ttttccaaat actccatctt tgaaatctgt tccagccagt ggattggata
     5521 gccaagcaag tccagccaca caaaaaagtt ttttagttgg aaatcaaaag gatatgcttg
     5581 gagattccac acgaaatgaa acatcattcc taaaaatttt ttcatactag cagaaagtag
     5641 atggccattt tcggtcagat aaaaagcttg ttcaaacagt ttaccataat ttgacaggtt
     5701 ttctgcctct aattgataaa aatagatcat ttcttcgatc atcccggcaa acggccgagt
     5761 cgttaattgc tttttatttg gatagttttt atacagattt tgcaaaaaac gatgatcaca
     5821 aatcattccg caacagataa aagtcaaaaa gtcaatctgc tttccgtcca cctgccgctc
     5881 gctgattgtt tgatcataaa cgatgcgttc tggcagattg atggtttgca gcactacacg
     5941 atttggtagc tgcaaaatcg tccgctgcgg tgttaatgct cggcttagag aacggatcga
     6001 tacagggatc atccatagtg atgaatcaag ggttgtatgt ttttgaaaaa aacctgccag
     6061 atccatgatc ccttcatttc caaatccgat gataaacatg gattgatttt cgggatagcg
     6121 tttgacgaaa tcaagcaggt tctttaattc tgttaaatga ttgcattgag tgttggcaca
     6181 aatataccag tcattgtttg cttgatttgc aaataacggg ttgatcttat ctgcaaacaa
     6241 atcataatag cgctgattcg ttaaaaataa gattggctga tcaatggaaa gttctcttag
     6301 ctgactgact agcgtttctc cgtaaaacac tttcgtcaac gcatcttcta cttgataatc
     6361 aagttccata cgcatccctc ctcttcttat ttttatttta ccattagtaa gcaaattcta
     6421 gaaaagataa gagcaaagct cacctcataa cttggaaata aataaaaatt aaataaaata
     6481 catgtgtttt ctatgtatga catacggatt cggaatctct gtctcttatt tattcctttt
     6541 ctgctaggaa ataaaaagaa aagcacttgt agccctagag ccacaagcgc tttttcaact
     6601 gtcttattta acagcgtctt ttaatgcttt acctggtttg aatgcaggta ctttgctagc
     6661 tggaatttcg atttcttcac cagtttgtgg gttacgacct ttgcgagctg cgcgttcgcg
     6721 aacttcaaag ttaccaaaac cgattaattg aactttttcg ccgttagcta aagcatcttg
     6781 aatagttgaa aatacagcat ctactgctgc agttgcatct tttttagtta aatcagtagc
     6841 tgaagcaact ttttcgatta attctgcttt gtttgccatg gatagattca cctcctccac
     6901 aagagatatg atagaaatat catttagtta aaactttctg agtggtccag aagtttaaaa
     6961 agaaatattg attaacttca atattctgtt atcacgatat catatgaacc gcttaatagc
     7021 aaggttttta ccaattttca catgcttttt ttagtcatag aaacgtaaca aacgaaactg
     7081 tctttttctt ttgaaaaaaa aacgtcgata tggtaaaatg cctattttct cctacgaggg
     7141 ataattcgga tggacgtccc ttcaaaagta aatgcttttc tgatttgatt ttctaagaaa
     7201 cgttcataag aaaaatgcat caattcctct tcattgacaa aaacgacaaa tgtaggtggt
     7261 ttgacagcga cttgtgtagc ataaaaaatc ttcaggcgtt ttcctttatc tgttggtgtt
     7321 ggattgattg ctacagcatc cataatgata tcattcaaca cagccgaaga gatacgaaga
     7381 ttctggttca tacttacttg ttcgatcatt tcaggcagac gttccaaccg ttgtttagtc
     7441 actgcagaaa caaaaacgat tggagcgtaa tcaagatacc ggaattcatc acgaatctct
     7501 tgttcgaaat cacgcattgt attggtttct ttctttacaa gatcccattt attcacgacg
     7561 ataataattc cacgaccagc ttcatgggcg taacccgcta ctcgcttgtc ttgttcccga
     7621 atcccttctt cagcattcag taccatcaaa acgatatcag aacggtcaat ggcacgcatt
     7681 gcacgcatca cgctgtattt ttcagtattt tcgtatactt ttccgcgttt acgcatacca
     7741 gctgtatcta tcattaagaa tttctgacca ctttcagaaa cgaagtgggt gtcgattgcg
     7801 tctcgggtcg ttccttcaat atccgaaaca attacacgtt cttctcctaa aatcgcattg
     7861 atcaaagagg atttaccaac atttgggcga ccaatcaaac taaacttaat cgtgtcgtcg
     7921 tcctcttctt cagtctcttc agagaaatac ttcactgctt catctaaaat atcaccaatc
     7981 cctagtccat ggcttcctga aacaggataa ggatcgccta gccctaagga ataaaattca
     8041 tagatatcgt ttctcatttc tggattatct actttattga ctgctaaaat cactggttta
     8101 ttacttctgt ataaaatttt cgcaaccatc tcatctgcgt ctgtcacgcc ttcgcgtccg
     8161 ctgactacac aaatgattac atctgcttct tcaatcgcga tttctgcttg atgcttgatc
     8221 tgatccataa aaggttcatc gccaagatca attccgcctg tgtcgatcac gctgaattct
     8281 cgacctaacc attcaccttt tgcataaata cgatcacgag ttactccagg agtatcttcg
     8341 acaatcgaga tacgctcacc agcgatgcgg ttaaaaatcg tagacttacc gacgttcggg
     8401 cggccaacga ttgcaattgt tggatttgcc atattatttc ctcctcacaa gtttccaatc
     8461 tcacttagtt taacgaatgc attgccaact ttcaagcata gattgtcaat taaacgaagt
     8521 atttttattc cttattgaaa tactaatcgc ttttcaaaag aaaaactgag acaaaagcag
     8581 ctgctttcat ctcagtcaca gatacaaaat caattatttt tcagaatcat cggttgcttt
     8641 ttcttctact tcttctttca acgcatcacc taaaatgtct cccatagtaa atccagtatt
     8701 ttcttctggt aattcataag actcaacatt ttttggttct tcttctgaag ctggtttttc
     8761 ttctaaagct ttgatactca atgcaatacg atgttcttct ggatggatct ctaatacttt
     8821 cactttcact tcgtcacctt catgaagcac ttcatgtggt gtcgcaatat gtttatgtga
     8881 aatttgagag atatggacta gcccttctac tccagggaac acttcaacaa aggcaccgaa
     8941 gcttgtcaaa cgtttaactg ttccgtctaa tactgtgcca actggagctt ttgtatcgat
     9001 ttctgaccaa ggtccaggct gtgtgtcttt gattgacaag gaaacacgtt cttgttcagg
     9061 attgatagac aacactttga catttacatc atcattgaca gccaatacat cacttggttt
     9121 tgctacatga ctatgtgaaa tctctgagac atggactaga ccgtcaattc cgcctaggtc
     9181 gacaaatgcg ccaaaatcag taagacgtgc aacacgtcca ctgagaacat ctccctcatg
     9241 gatagttgaa agaatttctt ttttcttttc ttctctttcg ttttccacta ccgctttatg
     9301 cgataagatc aaacgatttt ctgatggttc gatttcgatg attttaaagc tcaatgtttt
     9361 gcctttatag tctgaaaagt ctgcaacaaa atgatcttcc accattgatg cagggacaaa
     9421 accacgtaca ccgacgtcaa cgactaaacc gcctttgaca acatttgtaa caggtgcttc
     9481 gatgaccttg ccctcttgga aatctctttc gatatcttcc caaacttttt tagcatctaa
     9541 acggcgtttt gaaagtaggt agctgccgtt ttctttatct ttgccgatcg tactgataac
     9601 gactaaatct aatacatcac cgacttttac tacttcgctg atatcttcta ccggcaaagt
     9661 tgataattct tttgccggaa cgactccttc aactccagcc ccttcgattc cgacaatcac
     9721 ttgtttgtct tcaatgacta aaacttcgcc cttaacaaca tcgccaactt ttacttcttg
     9781 tacgcttttc attgcgtctt ccattgactg gttgttctct tgattttgat caaattctgt
     9841 cataacactt gtcctcctaa ccaacattct gccgtaagta cggtactgtt tttagtttaa
     9901 ttgattattc aaaaaaaata aagcgatttc gttcgttttc cttgaaaaaa caattgtttt
     9961 tcattttttt tcttaattta aaaaagaaga taaccttttt tcaaaacaac agcttttatt
    10021 gctgcaacga cttctggaat cgatttgcca gtcgtatcga tacgaacagc atcctctgct
    10081 tgcttcagcg gagagacgtc ccttgttgaa tcataatggt ctctttcctg tatctctttc
    10141 gtcagtgttt caagatcagt cggaattcct ttttcttggt tctctttgaa acgtctttct
    10201 gcacgctcac ttacgcttgc tactaaaaag attttcactt ctgcattcgg taatacagct
    10261 gtcccgatgt ctcttccgtc catgacaacg ccaccgcgtt cccctatttt tcgttggaca
    10321 gcaactaatt cttctctgac ttttccatgt gcagagactt cagaaacagc ttttgtgaca
    10381 tctggttgac ggatttctaa agtcacatct tttgatgcta tgaatacatg ctgtccatct
    10441 tcttgttgcg aaaaagagat aggatacttt ttgatcagcg aaactagccc ttcttcatct
    10501 gaaaaagata cattgttcgt aatagctaaa taagttactg cgcgatacat ggcaccggta
    10561 tctgtataaa tataattcaa ttcttttgct aatatcttgg caacggtact tttcccagat
    10621 gaagcaggcc cgtcgattgc aatactgatt tgtgtcatga tataactcct ttttctcaga
    10681 aaaaaagccg caaagcgcgg ctattattat ttaattctta attcttgacc agggtaaagc
    10741 ataaagttgt ttggatctaa gccattcaat tcaaacaatt gatcagtggt gatccctgca
    10801 cgctcagcca cttgttttgg accttcgcct gcttgaacag tcgttgttga accggctgca
    10861 gctgatgaag aaggtgttgt ttctgctgca ttgctgcttg aatcccctgc ttgataagtt
    10921 gcctcacttg aggaagtcgg tgttgtctct gctgcaatac tgctttctga cgttacacct
    10981 gtatcttgag acacagtaga ttcttccgcg attgtactag attctactgt tgaagtctca
    11041 gttgactcct cttttgttgt acttgtagaa ctttctacta ctgatgtcga agtttcagaa
    11101 gaagttgctg ctgttgcatt gttgcttgat ccatttctga tccagaaata agcacctgct
    11161 ggaatagcga tacataagac cagtaaaatc aaaaatagcg aaagaaataa tgtatttcct
    11221 ttcttttgtt gtctctgaga acttcttgat gaaacttcat catctgtatc gtagattggt
    11281 tgttcccatg gttcttgtgt ttcgttattt tctgtagatt gatgtctatc ttttctgctc
    11341 actgttttac ctcctaaact ctatcgtgtg tattatacca taggaaaaag tgtcttgtcc
    11401 taaaaaatca atgttccatg ttttttttaa ataaatttag taaaatcgtt tcccaatgaa
    11461 gttgttcagt cttagaaaaa agcttcctct ttggcagaag ttctgctttt gctccatcaa
    11521 tatcacaaca attctgcggc ttttcggaga gtttttcgcc aaaataagct aagatgaatt
    11581 ttctgcggca gttttcttca ttaatatagc gaagcatctg ctgcaggcgg aattcttttt
    11641 ctttttcctg acgtttgagc cgttccaccc aattttctgg tttttcagac tgctggatca
    11701 gctccatcca ttttttttgc agctcatcaa agggagcttg ctttgtggca tattccaaat
    11761 aaagctcaaa actttgtcgc tgttctctag aaagttgatt aaagaaataa tggatacgtt
    11821 catccccact ttgataaaaa agaattgctg cactttcttc ctgatctctg cctgctcgac
    11881 cgatttcttg tacatagttt tctagactgt ctggcaaatc ataatggacc acataacgga
    11941 tatcagattt atcgatcccc ataccaaatg cattcgtagc aatgagaaac tgcagctgat
    12001 tttttacaaa ctgctgttga agttgtcttc tttgagctgc atcaagacca ccgtgatagt
    12061 atccaactgt aaaacgttcc ctgaataaat gatacaatcg ttcgacttct ttttttgttg
    12121 cacaataaat gattgccgct ccttgtgctc tttccatgaa gtgttcaagt tcttgttctt
    12181 tttgctgcgt tttccgaaca aaaagcgaga tattttgccg attgactgat tgtctgatct
    12241 cttttggtgg tcgatcaaat agtacatgac gtatatctct tgctactaaa tctgttgctg
    12301 tcgccgttag tgccagtgtg accgggtttc ctaaaagctt tcggattttt cctaactgtt
    12361 gatattctgg acggaaatca actccccatt gagacacgca atgagcctca tctactacat
    12421 ataacgcaat attttgtttt ttcagatgct ctaaaagaga aggattagtc aacatttcgg
    12481 gactcaaaaa caaaaattta tattgcgata gatgacggag gacgtatctt ctttcagatt
    12541 gcgaaagtaa actattgaaa gcagctactc gtttctcccc tcgtttttgt aattgcgtca
    12601 cttgatcttc cattagagaa agcagcggtg acacgatgat gaccagacct tccattaaat
    12661 accccgtcaa ttgatagcaa aggctttttc ccgttcctgt aggcaaaata gcgagagtat
    12721 cttctccatc aagcaacgcc tgaataattt cttcttggcc gggacgaaat gaatcaaatc
    12781 caaaatattt ttttaattca tttattaaca ttctgtatcg cttcctttct caatagatag
    12841 atttgataca aacgaaattc tccataatct aattgtccat ctacattcca ttcgctaaag
    12901 cgccactctc gcacggaatc cttatttggt tccaaacgtt cgattgtttc tgcagagaga
    12961 atccactcgt atggaaaatc atcaaaaaac aaggcccact caataagatg atctgttact
    13021 gttcctcttt ttaaatgacg gattgccatg atctggtcaa ttgtctgacc attcataaac
    13081 atctgcttgg tgatcatcat gctttgattg aaattttgct gtaataatga atcgatcaaa
    13141 gcgtgccaaa gcgaatctgg ccgtttttcg atttgtgcta acaaaaggtg gatacatctg
    13201 ctttgttgca gataaacctc tgactcatca ttgttttttg ccagctgata aggaagcagc
    13261 cctgttcgct catttccaga taattggtta gctaaaaaat ctgctgcttc ttctggtatc
    13321 tccgaaaaca gctgcaccaa actctcatac gcagtatcaa tcaaaatttc acgtggcaat
    13381 tggctttcac taagccattt ttttagttgg aaagtataaa aaggacttgt ctctgctgga
    13441 agatagtctt gaatacccgt tgccagatta gaaattactt gaacagcaaa tttaatgagc
    13501 cgccaactag tttcccctgt tctgccatag cggtcaaaac gcaaaccagt atgttctatt
    13561 tgatgttcgg acaacatatc agcccctgca ggcgttagct ttgcttcatt ttcatttatt
    13621 tcgatccaac cttgctgcgc aattttttgc aaaatttgat aaaacttatc ctgcttcaat
    13681 tctggaaaac tgttatggat aaacaaaagt tcgtgggtaa atccataaat aagtactgaa
    13741 gtcgttcgtt ttcccacaag gagttggtaa agagaactaa tccgcagctt gtgtcctgat
    13801 gaaaatagcg ataaaataaa aacaatcata ttattctcct taagttagga tggttagaaa
    13861 tgtcactact atgtaaactt gtacctgaac gctgtattgc atgcggtctt tgtcaaatcg
    13921 aagcgccaga tattttcgat tatgatgaag aaggaatcgt tttatttgcc caagaaccag
    13981 aggctcatca acagttcgtg atgccggaag aagaaacata tgtaaaaaaa gcgtatcaaa
    14041 aatgtccagt tcgagcgatt ttattagaaa ataaaatata aatataaacg aaactttaaa
    14101 aacgtacact aagttctcta aacttgaaaa taagccggga tctgtttctc ttgttccgtg
    14161 aaatcccgac ttattttgaa agattcattt gctatctata gttaattgac tagttttttc
    14221 acggacccta taggacccat ttatattatt tcaacttttt cagccagttt tccaatgtat
    14281 tgacgatcac agtttgctgg tctgcgttag aaatttctgc ctctccatct cctttttgct
    14341 caccatagct gccaaaacct ccatgatttc cgccattgat tgatacatac tctgttcttt
    14401 ccggtaaata ctgcttagct tcttgataag cttcctgatt caatacttta tcttttgtcg
    14461 ccgtgatcga taaaacagga acagctgatt ctgtcaatgt tccttttttg tcgggataac
    14521 ttgccatgaa gaatacccct tttaaatgtt tgtctgaatg attcttattg acataacgac
    14581 ttgccattac gccacctaat gaatggccac cgatcacata atcggaattt ggaaaatctt
    14641 ctttgacctt atccgctttg tttccgccca tcactgctag atcaagtggc attttgacaa
    14701 tagcgactga aaagccgttt tttgctaaat cttgcgccca aatactataa ctttctggct
    14761 ctacgagagc accaggataa aaaataagta atggagactt tccatgattc tttttctctc
    14821 cttcaaaaaa taagtaatct tctttgtttt ttccttcttt taccaacact tgcgcttctg
    14881 acgatggttc gtacgtattt tgctgtatat agtaataacc ggctccaccg agtaatccta
    14941 aaatgatgac taagccaatt agtattcgtc taaatatttt cactttctcc caccttttct
    15001 atctgagtga cccctttttc ttaatcaagt atgattatac cttaaaaaaa taagagagaa
    15061 atgcaaactt tccagcatca aataaaaaaa gacggaatat ccatttgaat atccgccctt
    15121 tttgtatgtt tatttttatt tagcacataa acgatacaca gcttctgcat aaatttccat
    15181 tgctttatac atactactta attcccatcg ttcattcgcc tggtgcataa agtcaggagt
    15241 atcagggaac atcgcaccga atgccacaca ctggttcatc gtacgagcaa aagtagcgcc
    15301 gcctgataca aacggttcag tcatgtctcc agtaatatcg cgatatgtcc ccaatagatt
    15361 tttcactaac tcactgtcta gtggcacgta taatgaatca aggaagtcaa attcttcata
    15421 agtcaaccca tatttttctg ctgttttagt taatttagct actaaatcat ctttcttgaa
    15481 tgtaacaggg attctcatat ctacaccgat ttttgtttgt tctggtgtga tttccaaact
    15541 tgcaaagttc atcgttagct cgccggattg ttcatcttct gttttgccca cgacagcttc
    15601 accagttgca ttttcttgaa ctaattttcc taagaaattg attgggccaa aatcaaatac
    15661 ttctgaaaga gcaattgcca gacgagaaat agcattcact ccttgtgctg catctttcgc
    15721 gtggatactt ttaccaagaa cgacgatgcc ttcaccttgt tcttcgaaat cgaattcgtg
    15781 ttttttcaat gcttctttta cttcagaaag cttttctcca gagtaaggag ctgcatcagg
    15841 aacaacattc aacgctcctc cagcttttac agaaaactcg ttcgttcctg gtcctgtcaa
    15901 gtaagcttgc aataagcctt tttctgcata aattaaagga aattcagcat ctggcgcaaa
    15961 tccttgcgta attccttctt ctttttcgtt atatttttct aaacaacgcc ataaattttc
    16021 ttcatctgta ccaaagataa aacgaatacg tgtcttgaat tccacgccag catcaatcaa
    16081 agctttgacg gcatacattg ccgcaataga aggacctttg tcatcttggg atccgcgacc
    16141 aactaaccag ccgtctttta tagttggatc gaatggtttt gtttcccaat tattttcgtc
    16201 ccctgctgga acaacgtcca tatgacacaa gataccgaat aattcatctc cagagccaat
    16261 ctctgaataa ccgtaatagc cttctggatc tttaaatgtt ctaaatccta gcttttcact
    16321 gatttccaat actttatcca aagcttcaat cactttttta ccgaagggat gtccttgtcc
    16381 tgaatctgct tcatctagta cggaaggaat tcggatcaat tcgctcaatg attctaatgc
    16441 ttgttcatga tgttgttctg tcacaaattt tttcatgata ttatcctcct aatatgttaa
    16501 aaagggtcat gtcaaattcc tccgacacaa cctcttcttc tcaaataatg acgttttaag
    16561 cctgctccat cagcaagcag aatgttctta acaaatgatg aaagaaaaaa cactcactga
    16621 atggagctat tgacttgtcc acgtgcttat cgatagccgt ttatcggttt cttcattctt
    16681 atgatagacc ttgtacctgt atttgtctag gtttagaaca ttgctgctac acctaggatc
    16741 acacagatgg ctacaaataa tatcgcaatt aatttaccgg taaatttcca ccacactgaa
    16801 acatcgaaac gagcaattgc tacagcaccc attacgatag cagatgttgg tgtaattaag
    16861 tttactaaac cagatgcaga ttgataagca gtgattacta aatccttacc gacgccggca
    16921 aattcaccca taggtcccat gattcccatt gttgcagcag ctaagccgga agttgaagga
    16981 atcaagaatg acatagggat atagaaaata aatgtaataa tgatgaatac acttgaagat
    17041 aatgaactta atccttgttc gccccaatgt aagatggtgt ctgtgattaa tccattgttc
    17101 ataaccactt ggataccacg agcaacagct actacgattg ctacaccgat caaatcagaa
    17161 gcaccagcta aaaattcaga gacaaattca gactctttca tgccataagt gattgctact
    17221 acgatggaca ttactaagaa taacatagta atttctggga agtaccagtc acctaatgga
    17281 agcatgcttt tacctagaac cgtacctagt accggaaggt ttgttaacca gtttgtcaat
    17341 gattcaaaaa tcgtgaaatt cgcattcaag gttgtccaag gaatcaaact gatgatcatg
    17401 ataccaaaag taataaagaa caatacatta acatgttttt gttttttcgt gattacttct
    17461 tgacgaccga ttgcttcaat atcgaaatgc ttcatatctt ctttacgttg gttgtatact
    17521 aatgattttg ttggatcttt ttcgatcttt gatgcataac gatacacata tacaatagaa
    17581 atagctaaca aaataacgaa catcaagaat cgaagaccaa taccttcacc catcgaaatc
    17641 ccaacagctt gtgaagctac gcctgttgca aaaggattta ccgttgaagc tagacagcct
    17701 acttgagacc ctactaatac gattgctaca gctactactg tatcaaagcc tacagccatc
    17761 atgactggga tcagtaaagc atagaatgga atcgtttctt ctgccattcc gtaagtagag
    17821 ccgcctaatg caaataacag catcaatact ggaataagca ttttttcttt tcctttgaat
    17881 ttcttcacaa ctgaagcaat cccggcatct agtgcccctg ttttgttgat aatacctaaa
    17941 aatccaccaa caaccaaaat gaagaaagag attgggattg cagcaggtgt ttctttagtt
    18001 agctcccccg ctggtacacc tagcatccca ttgattggcg ccatgaaaat atcccacagc
    18061 ccttgaggat tttgttgtac tcgttcatat gatccagcga cgatgttgcc tgcatcgtct
    18121 acactatagt gcccagctgg tataaaccaa gtaagtacag cgattactgc aataataata
    18181 aataagaccg tatacgctga aggcatttga aattttttct tttttgtttg ttccatcttc
    18241 ttcttccttt cttttccgtt agagacaaca attttggctc taatgttatt ttatcaaaaa
    18301 gagtatcaga acaaaaataa taacacgcat tttgtaaaac ttttgttcaa aaagttcgtt
    18361 tttccctatt ccctcctctt ttttcagttt tttaaagctt tcttcttttt cattgtagcg
    18421 tttccttttt tttataaaca aacattttcg tcttttaaaa ggagaagaat ttcacatttg
    18481 caaagaattg attgattttt atgcataaat caaaaaaata ctcaaaacaa acaaataaaa
    18541 cgtttacatt attgcatgtc ttgtattttt ttccaaaaaa aaagaccaag aaatttcttg
    18601 atctttttga tatttatttg actgctgtat gacgttgttc gaactgatgt tgttttcgac
    18661 ttaaccatgg aagaagttgc gcatgcagca ctaaaaagac agcactcacg atgaatcctt
    18721 tgatcaagtt gaaaggaacg atcccaatca gtacatagtt tgctaaagac atgcccagca
    18781 tctgtgttgc gttcagccct aaaaacatta agtaaagagg cgtaataacg aaataatttg
    18841 cgatactcat aaaaatagtt agagcgatgg ttcctgtaat gacacctaaa aatttgtttc
    18901 ttcctttatg ctgtttcttt acactaaaga aataataaat cgggaatgta aagatcgttg
    18961 ttgataagaa actagcagtg tctcctacca aattatccgg agaaaaacct gtagtcagca
    19021 gatgtaatac tgaacgcaag aacgctgttg caactccggc cgctggaccg aataagaaca
    19081 tgctgatcaa taccgagata tcactaaaat cgatcttcaa aaaactaaaa gccggcatga
    19141 tcggaaaagc aaaatactgt aaaactaatg cgatcgcagc aaacatcgct actgcaacca
    19201 ttttctgtac acgggtgttc ttcatttgaa atcctcctgt taggactcct cttcagcgaa
    19261 ctttttgacg tttctctcca aatgaaaaaa ctctattttc cagggaaaat agagttagag
    19321 ttatttgatc aagcgtcgtc aaataagctc tatcttcttt atccagacta tactgtcggt
    19381 atcggaattt caccgattca gctatttttt tgcaaaatag gtcgtggact ataaccaccg
    19441 gtcgggaatt tcaccctgcc cctgaagacg aaccttatat aatttttgtt tacatattca
    19501 ttatacatat ctattcaaaa aacgcaacat ctaacaatta taaaaagtaa gtgttcattt
    19561 tgagtggttc agcaaactgc tgacttcttt tttgttcagt tcacggtaat ccccaggacg
    19621 taggccttgt aaagtcagat ctccgtattt ttcacgtttt agtttttgca caggaagacc
    19681 aactgcttga agcatatttt tcacttgatg attacgtcct tcgtggatcg tcaattccac
    19741 gatactgtgg tttgtcttag gatccactga taaaatttgg aatttagctg gagatgtttt
    19801 tcgtccttcg atctttattc cttttgtcaa aggtgcaagc atcgattttg tagcgatccc
    19861 ttttaccttt gcaacgtaaa ctttatccac ttcatgtttt ggatgtgtca ggcgctgagc
    19921 aaaatctcca tcatttgtca gtagcaagat ccccgaagta tcgtaatcca gtcgacctac
    19981 aggatagata cgttctttta ccatcggcaa aagatccgta acaacttttc ttcctttgtc
    20041 atctgcaaca gcagaaataa ctcctttagg tttgtacaat aaatagtaac cgtattcttc
    20101 ttgataaatt gggacttcat ccacttccac agtgtcatgt ttcccaactt ttatccctaa
    20161 ttcccttaca acttgtccgt ttacttttac acgtcctgca aggatgaatt cttctgcttt
    20221 tcgacgagaa gtgatgccag cgtgggcaat tactttctgc aatctctcca ttacaattat
    20281 tctcctttat tcattccatc aaagaataga tctggtgaca cgtcttcttc aagtgaaatt
    20341 tccatttctt gtgtatcagg cagttcttct aatgatttca aaccaaaata atctaagaag
    20401 tagccagttg ttccatataa tatcggccga cctggtcctt ctactctacc tttttcttca
    20461 atcagtttat gggcagctag acgctgtacc gctcctgaaa cctgaactcc tcgaatctgc
    20521 tcgatttcag ctcttgtgat tggctgttta taagcgatga ttgctaaggt ttcgacggcg
    20581 gcttgagata atgtgtttga tgtcccttgc gcatatcgct taagtaaggg agccagctct
    20641 tttttagtcg ttaatacata atggctttcc gtttcaaaaa tatgcaaagc tgagtgcgag
    20701 ttttgagtgt aagattcttt taattctttc actaaacttg tgacttctgc ttcgctgaga
    20761 tttaacagtc cgcttaattc ttcattactg atcccttcct cgccaacaat aaaaagaagg
    20821 gcttcaagtt cacttaattt cgtcatgttc ggtatctcct actgctggat aaagcataat
    20881 tgattcatag ttttcttttt gttgcgcaat gatctgccct tttttcatca attctagcaa
    20941 agccataaaa gtagtgacaa cttcactttt agaaggtgac tcaaaaaaag aatcgaaagt
    21001 acagccatgc gcatttcttt ttatacgctc ttcgataaat accattcgtt cttcgatcgt
    21061 tgtgtcatcg ggcgtgatcg ttttttccac tttttgagaa aatttcttac gttctaagat
    21121 tttctgcatg gcacggatca aatcgatttt tttgacttgt cctcgcttca acggcttttc
    21181 agcatccgct aacccatcga tgttcatagg ttcttttgtg taataaagac ttcgttcctc
    21241 tgctttttct gtcagtcttt cagctgcata tttatattta cggtattcta aaagctgagt
    21301 gaccaattct tctcgaggat cttcttccca taattcttca tcgtctgtgc cgaattcttg
    21361 gacaggaagc aatgtcttgc ttttgattgc catcaatgta gcagccatca ctaagtattc
    21421 tcctgcaaca gctagctcga gtgttttcat tgtatggata taattcatat attgctccgt
    21481 gatcgtagcg atcggtatat catatatatc gatttccatt tgctgaatca gatgaagcag
    21541 cagatcgaga ggaccctcga atacatctaa tttaatattg atctccgtca actcattcac
    21601 tctttcttct ttttctttgc tagccatttt ccaatgtaag gctctgtgat ttcagttgca
    21661 cggatggaga catccgggta cttttcctgt aattcatcta acatttgaaa gcccatattc
    21721 tcaccgcgat aagacggatt caaagaaatg tcatggatga tcagctgatc gttcgttgcc
    21781 cagtaaatac caataatccc ttgaatgttg gttgaacctt ctggcgtaaa gaaaaaaagt
    21841 tcataatcgt cttcagattc gtacgtatca atctccttca acaaagaagt tttacctgtc
    21901 agtttcttat ggaaactcaa tagtcccata gcaattttac ggttttcttt ccggtaatgt
    21961 gttaacatct tctcaccttc ctatgactaa gcccttggaa aatgctgctt ataaacatcc
    22021 gtcattcttt tcttcgtgat atgtgtataa atctgagtcg tagagatatc cgcgtgacct
    22081 aacaattcct gtactgtccg caaatcagct ccattttcta gcagatgtgt ggcaaagcta
    22141 tgccgcagcg tatgtggtgt tacttctttg taaatcccag cctctcgtac taactgcttc
    22201 agatttttcc agattccttg ccgagaaaga cctttcccat gattattgac aaatacatgc
    22261 atttcagatg cgtcctttac aagtaatgga cgtgcttcgt ctaagtagcg ttccagccat
    22321 tggatcgcat aatcacctaa aggaatgatc cgctctttat ctccttttcc aactgtctgt
    22381 accagtccaa gagaaagatg aagatctccc aatttcaatc caatcaattc actgacgcgc
    22441 atcccagtag catacatgac ttccaagatt gcacgatctc ttatccctaa cgtttttgtc
    22501 gtatcaggtg cttcaatcag cttttctact tctgtcagag tcaatgtact tggtaatttc
    22561 tgtgctttct tcggcgtatc gatatgttgc atcggatcat gatcagtgat acgctcttgc
    22621 cgtaaaaact gatggaatct tctgagactt gagatcatcc gagtaacagt tgctgtcgct
    22681 tttttttctt catgcaactt ctccagaaaa cggatgacta aaaagcgatc gacatcctgc
    22741 catgaagcaa ttttctgctc ctgtagaaaa tgcaagtact gttccaaatc acgttcatag
    22801 ctttgtcgtg tattggcgga caatccccgt tcaatattca agtagtggag ataatcgttg
    22861 atttgctctt tcataataat ccccctcatg cctctctatc atatcagata ccgcttcatt
    22921 ctgatagtct ttttccataa tctgagcaaa gaaaaagccg tctagacact cagacggact
    22981 ggtttctttt ttcggctcta taatttatgg gactgatgaa tcagaattga ttcttcagcc
    23041 ccattttcat tttaggtatt aaaaaa
//
LOCUS       DACDXE010000046        22457 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.046, whole genome shotgun sequence.
ACCESSION   DACDXE010000046 DACDXE010000000
VERSION     DACDXE010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.046"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..152
                     /locus_tag="IWM23_001763"
     CDS             <1..152
                     /locus_tag="IWM23_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9884397.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="IWM23_001764"
     CDS             complement(230..763)
                     /locus_tag="IWM23_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAP9884398.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWM23_001765"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="IWM23_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAP9884399.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWM23_001766"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="IWM23_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAP9884400.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="IWM23_001767"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="IWM23_001767"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAP9884401.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="IWM23_001768"
     CDS             complement(4488..5066)
                     /locus_tag="IWM23_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAP9884402.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="IWM23_001769"
     CDS             5388..5858
                     /locus_tag="IWM23_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAP9884403.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="IWM23_001770"
     CDS             complement(5966..7192)
                     /locus_tag="IWM23_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAP9884404.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="IWM23_001771"
     CDS             complement(7946..8146)
                     /locus_tag="IWM23_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9884405.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="IWM23_001772"
     CDS             complement(8280..8888)
                     /locus_tag="IWM23_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAP9884406.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="IWM23_001773"
     CDS             complement(8973..9443)
                     /locus_tag="IWM23_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAP9884407.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="IWM23_001774"
     CDS             complement(9491..10405)
                     /locus_tag="IWM23_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAP9884408.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="IWM23_001775"
     CDS             10551..11354
                     /locus_tag="IWM23_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAP9884409.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="IWM23_001776"
     CDS             11532..12479
                     /locus_tag="IWM23_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAP9884410.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="IWM23_001777"
     CDS             complement(12547..12840)
                     /locus_tag="IWM23_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAP9884411.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="IWM23_001778"
     CDS             complement(12868..13533)
                     /locus_tag="IWM23_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAP9884412.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="IWM23_001779"
     CDS             complement(13673..14305)
                     /locus_tag="IWM23_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884413.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="IWM23_001780"
     CDS             complement(14312..15379)
                     /locus_tag="IWM23_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9884414.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="IWM23_001781"
     CDS             complement(15376..16107)
                     /locus_tag="IWM23_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAP9884415.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWM23_001782"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="IWM23_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAP9884416.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWM23_001783"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="IWM23_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAP9884417.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="IWM23_001784"
     CDS             18270..19439
                     /locus_tag="IWM23_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9884418.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21328)
                     /locus_tag="IWM23_001785"
     CDS             complement(19706..21328)
                     /locus_tag="IWM23_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884419.1"
                     /translation="MNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAFHINR
                     ALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLYIVTL
                     ITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPLILLG
                     FIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIFLIIK
                     LIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDSELLS
                     FGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLNIIQA
                     PWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNMVKEM
                     NFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPNKDEI
                     IFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTIRYSY
                     TFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
     gene            21825..22457
                     /locus_tag="IWM23_001786"
                     /pseudo
     CDS             21825..22457
                     /locus_tag="IWM23_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT         6972 a         4369 c         3555 g         7561 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatataa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtttttttat attacatata
    21361 gtatcattta tttttttaaa acactagatt actcactata taaaatgtga ttaattttat
    21421 tattaataaa atatttttta tattacaaaa gtagtgtttc aaagttagta gagaaaatgt
    21481 agttatatat ttaaattttt accagaaaga aatgaacacc ttcaattatc taaatctcga
    21541 aaatggttag atgtaatcat caggaaaaac aatcttaaac gaattactat tcatggtctt
    21601 agacacacac atgctagctt gcttttagca agctagtgca aatattaaag atgtgcaaga
    21661 gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata ttacagacaa
    21721 aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt tatgggactg
    21781 atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt cgtacattga
    21841 aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa gtattgatgg
    21901 tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt tgtgcggatg
    21961 gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga ttaacacact
    22021 attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat atgaacgccg
    22081 cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt gattgttttc
    22141 atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa atgaaaagat
    22201 tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg cgctttcttc
    22261 taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt tttcgtgcac
    22321 ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca ttttcaacgc
    22381 ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga gacaaggaat
    22441 cagacttatt ttttacc
//
LOCUS       DACDXE010000047        22031 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.047, whole genome shotgun sequence.
ACCESSION   DACDXE010000047 DACDXE010000000
VERSION     DACDXE010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.047"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            367..555
                     /locus_tag="IWM23_001787"
     CDS             367..555
                     /locus_tag="IWM23_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884420.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="IWM23_001788"
     CDS             545..805
                     /locus_tag="IWM23_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884421.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="IWM23_001789"
     CDS             846..1073
                     /locus_tag="IWM23_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884422.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="IWM23_001790"
     CDS             1138..1710
                     /locus_tag="IWM23_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884423.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="IWM23_001791"
     CDS             1711..2022
                     /locus_tag="IWM23_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9884424.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="IWM23_001792"
     CDS             2013..2273
                     /locus_tag="IWM23_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884425.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="IWM23_001793"
     CDS             2434..2793
                     /locus_tag="IWM23_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884426.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="IWM23_001794"
     CDS             2790..4433
                     /locus_tag="IWM23_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9884427.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="IWM23_001795"
     CDS             4468..5550
                     /locus_tag="IWM23_001795"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884428.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="IWM23_001796"
     CDS             5594..6766
                     /locus_tag="IWM23_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9884429.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="IWM23_001797"
     CDS             6753..7457
                     /locus_tag="IWM23_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9884430.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="IWM23_001798"
     CDS             7462..8622
                     /locus_tag="IWM23_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9884431.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="IWM23_001799"
     CDS             8698..8976
                     /locus_tag="IWM23_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9884432.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="IWM23_001800"
     CDS             8957..9328
                     /locus_tag="IWM23_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HAP9884433.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="IWM23_001801"
     CDS             9344..9748
                     /locus_tag="IWM23_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884434.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="IWM23_001802"
     CDS             9745..10155
                     /locus_tag="IWM23_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884435.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="IWM23_001803"
     CDS             10216..10803
                     /locus_tag="IWM23_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9884436.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="IWM23_001804"
     CDS             10876..11343
                     /locus_tag="IWM23_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HAP9884437.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="IWM23_001805"
     CDS             11389..11697
                     /locus_tag="IWM23_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884438.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="IWM23_001806"
     CDS             11892..18626
                     /locus_tag="IWM23_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9884439.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="IWM23_001807"
     CDS             18630..19502
                     /locus_tag="IWM23_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884440.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20126
                     /locus_tag="IWM23_001808"
                     /pseudo
     CDS             19499..20126
                     /locus_tag="IWM23_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20087..20548
                     /locus_tag="IWM23_001809"
     CDS             20087..20548
                     /locus_tag="IWM23_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884441.1"
                     /translation="MQSPGPGNENKLLIVDKAMKDIENPLVLSTWYLDDENNLTQDST
                     NKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGNAYSHEIKVNVVLNARNINVENI
                     ETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFGNVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="IWM23_001810"
     CDS             20552..21376
                     /locus_tag="IWM23_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884442.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..21939
                     /locus_tag="IWM23_001811"
                     /pseudo
     CDS             21394..21939
                     /locus_tag="IWM23_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
BASE COUNT         7878 a         3297 c         4670 g         6186 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacttataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DACDXE010000048        22028 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.048, whole genome shotgun sequence.
ACCESSION   DACDXE010000048 DACDXE010000000
VERSION     DACDXE010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.048"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..1371)
                     /locus_tag="IWM23_001812"
     CDS             complement(61..1371)
                     /locus_tag="IWM23_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9884443.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="IWM23_001813"
     CDS             complement(1383..2801)
                     /locus_tag="IWM23_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9884444.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="IWM23_001814"
     CDS             complement(3041..4609)
                     /locus_tag="IWM23_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAP9884445.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="IWM23_001815"
     CDS             complement(4613..4894)
                     /locus_tag="IWM23_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAP9884446.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="IWM23_001816"
     CDS             complement(4891..5814)
                     /locus_tag="IWM23_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAP9884447.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="IWM23_001817"
     CDS             complement(5811..6689)
                     /locus_tag="IWM23_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAP9884448.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="IWM23_001818"
     CDS             complement(6676..8013)
                     /locus_tag="IWM23_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAP9884449.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="IWM23_001819"
     CDS             complement(8016..9119)
                     /locus_tag="IWM23_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAP9884450.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="IWM23_001820"
     CDS             complement(9193..10701)
                     /locus_tag="IWM23_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9884451.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="IWM23_001821"
     CDS             complement(10667..11359)
                     /locus_tag="IWM23_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9884452.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="IWM23_001822"
     CDS             complement(11381..12355)
                     /locus_tag="IWM23_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884453.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="IWM23_001823"
     CDS             12655..13527
                     /locus_tag="IWM23_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884454.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="IWM23_001824"
     CDS             complement(14109..16307)
                     /locus_tag="IWM23_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9884455.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="IWM23_001825"
     CDS             16539..17531
                     /locus_tag="IWM23_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884456.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="IWM23_001826"
     CDS             17591..19012
                     /locus_tag="IWM23_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAP9884457.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="IWM23_001827"
     CDS             19005..20504
                     /locus_tag="IWM23_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAP9884458.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="IWM23_001828"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="IWM23_001828"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAP9884459.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7041 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaat
//
LOCUS       DACDXE010000049        21912 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.049, whole genome shotgun sequence.
ACCESSION   DACDXE010000049 DACDXE010000000
VERSION     DACDXE010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21912
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.049"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM23_001829"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="IWM23_001829"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAP9884460.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="IWM23_001830"
     CDS             complement(1172..1867)
                     /locus_tag="IWM23_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAP9884461.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="IWM23_001831"
     CDS             complement(1880..2596)
                     /locus_tag="IWM23_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAP9884462.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="IWM23_001832"
     CDS             2926..3534
                     /locus_tag="IWM23_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAP9884463.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="IWM23_001833"
     CDS             3859..4182
                     /locus_tag="IWM23_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9884464.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="IWM23_001834"
     CDS             4364..5734
                     /locus_tag="IWM23_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAP9884465.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="IWM23_001835"
     CDS             complement(5864..7165)
                     /locus_tag="IWM23_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9884466.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9510)
                     /locus_tag="IWM23_001836"
     CDS             complement(7519..9510)
                     /locus_tag="IWM23_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAP9884467.1"
                     /translation="MATTHNTKKISMTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="IWM23_001837"
     CDS             complement(9654..9920)
                     /locus_tag="IWM23_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAP9884468.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM23_001838"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="IWM23_001838"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAP9884469.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM23_001839"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="IWM23_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAP9884470.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="IWM23_001840"
     CDS             13264..13827
                     /locus_tag="IWM23_001840"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAP9884471.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="IWM23_001841"
     CDS             complement(14080..14601)
                     /locus_tag="IWM23_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAP9884472.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="IWM23_001842"
     CDS             complement(14604..15446)
                     /locus_tag="IWM23_001842"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAP9884473.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="IWM23_001843"
     CDS             complement(15439..16164)
                     /locus_tag="IWM23_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAP9884474.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="IWM23_001844"
     CDS             16340..16636
                     /locus_tag="IWM23_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884475.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="IWM23_001845"
     CDS             17204..18796
                     /locus_tag="IWM23_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAP9884476.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="IWM23_001846"
     CDS             18809..19549
                     /locus_tag="IWM23_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9884477.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="IWM23_001847"
     CDS             19717..21066
                     /locus_tag="IWM23_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9884478.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="IWM23_001848"
     CDS             complement(21194..21784)
                     /locus_tag="IWM23_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAP9884479.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
BASE COUNT         6295 a         4462 c         4158 g         6997 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aa
//
LOCUS       DACDXE010000050        21315 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.050, whole genome shotgun sequence.
ACCESSION   DACDXE010000050 DACDXE010000000
VERSION     DACDXE010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.050"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM23_001849"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="IWM23_001849"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAP9884480.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM23_001850"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="IWM23_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAP9884481.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM23_001851"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="IWM23_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAP9884482.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM23_001852"
     CDS             complement(3742..5853)
                     /gene="hflB"
                     /locus_tag="IWM23_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAP9884483.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM23_001853"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="IWM23_001853"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAP9884484.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM23_001854"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="IWM23_001854"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAP9884485.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="IWM23_001855"
     CDS             complement(8053..8523)
                     /locus_tag="IWM23_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAP9884486.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="IWM23_001856"
     CDS             complement(8569..8994)
                     /locus_tag="IWM23_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAP9884487.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="IWM23_001857"
     CDS             complement(9061..9330)
                     /locus_tag="IWM23_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAP9884488.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="IWM23_001858"
     CDS             complement(9341..10936)
                     /locus_tag="IWM23_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9884489.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM23_001859"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="IWM23_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAP9884490.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="IWM23_001860"
     CDS             complement(14489..15049)
                     /locus_tag="IWM23_001860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAP9884491.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="IWM23_001861"
     CDS             15402..16376
                     /locus_tag="IWM23_001861"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAP9884492.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="IWM23_001862"
     CDS             16546..17946
                     /locus_tag="IWM23_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAP9884493.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="IWM23_001863"
     CDS             complement(18024..18746)
                     /locus_tag="IWM23_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAP9884494.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="IWM23_001864"
     CDS             complement(18825..19331)
                     /locus_tag="IWM23_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAP9884495.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="IWM23_001865"
     CDS             19454..21253
                     /locus_tag="IWM23_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAP9884496.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5929 a         4672 c         3841 g         6873 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaat
//
LOCUS       DACDXE010000051        20948 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.051, whole genome shotgun sequence.
ACCESSION   DACDXE010000051 DACDXE010000000
VERSION     DACDXE010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.051"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..333
                     /locus_tag="IWM23_001866"
     CDS             <1..333
                     /locus_tag="IWM23_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9884497.1"
                     /translation="LTTGADGKLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKE
                     QEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISI
                     KFRKKRNI"
     gene            complement(757..927)
                     /locus_tag="IWM23_001867"
     CDS             complement(757..927)
                     /locus_tag="IWM23_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9884498.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1121..1300)
                     /locus_tag="IWM23_001868"
     CDS             complement(1121..1300)
                     /locus_tag="IWM23_001868"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884499.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            1687..2799
                     /locus_tag="IWM23_001869"
     CDS             1687..2799
                     /locus_tag="IWM23_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9884500.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(2877..3170)
                     /locus_tag="IWM23_001870"
     CDS             complement(2877..3170)
                     /locus_tag="IWM23_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAP9884501.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3338..3652
                     /locus_tag="IWM23_001871"
     CDS             3338..3652
                     /locus_tag="IWM23_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAP9884502.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3673..5652
                     /locus_tag="IWM23_001872"
     CDS             3673..5652
                     /locus_tag="IWM23_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9884503.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5665..5991
                     /locus_tag="IWM23_001873"
     CDS             5665..5991
                     /locus_tag="IWM23_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAP9884504.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6007..6525
                     /locus_tag="IWM23_001874"
     CDS             6007..6525
                     /locus_tag="IWM23_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884505.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6525..7877
                     /gene="celB"
                     /locus_tag="IWM23_001875"
     CDS             6525..7877
                     /gene="celB"
                     /locus_tag="IWM23_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9884506.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(7961..9466)
                     /locus_tag="IWM23_001876"
     CDS             complement(7961..9466)
                     /locus_tag="IWM23_001876"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAP9884507.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9558..10148)
                     /locus_tag="IWM23_001877"
     CDS             complement(9558..10148)
                     /locus_tag="IWM23_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9884508.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10327..11973
                     /locus_tag="IWM23_001878"
     CDS             10327..11973
                     /locus_tag="IWM23_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9884509.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12022..12741
                     /locus_tag="IWM23_001879"
     CDS             12022..12741
                     /locus_tag="IWM23_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAP9884510.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13220..14167
                     /locus_tag="IWM23_001880"
     CDS             13220..14167
                     /locus_tag="IWM23_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884511.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14179..15099
                     /locus_tag="IWM23_001881"
     CDS             14179..15099
                     /locus_tag="IWM23_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884512.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15140..16597
                     /locus_tag="IWM23_001882"
     CDS             15140..16597
                     /locus_tag="IWM23_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9884513.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            16942..17556
                     /locus_tag="IWM23_001883"
     CDS             16942..17556
                     /locus_tag="IWM23_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9884514.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17549..19273
                     /locus_tag="IWM23_001884"
     CDS             17549..19273
                     /locus_tag="IWM23_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9884515.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19270..20721
                     /locus_tag="IWM23_001885"
     CDS             19270..20721
                     /locus_tag="IWM23_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884516.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6760 a         3542 c         4190 g         6456 t
ORIGIN      
        1 ctaaccacag gagcagatgg aaaactggcg atagatggct tagcaccagg agcatatcag
       61 ctggtagaaa ctcaagcgcc aacggggtat gaattggatg cgacaccagt aactttcaag
      121 atcgaaaaag aacaagaagt ggcgatcttt ttaacaaaag agaatagaaa agttgctgat
      181 agctcagata taagaggaaa caatccaaca agcagtggaa ataagcatct tccaaaaact
      241 ggagaaacca tcattagcca gtttatattg tcaatattgg gtgtgctact tgttttcatc
      301 agtataaaat ttagaaagaa acgaaatata tgagctaaaa taaaaaattg aataagcata
      361 gtttttaaaa tcaactagta cccatcaatt gaatggaaac taccgatttt agaaggggta
      421 tacaaacaaa tattagtatc ggagtagaga aggttgaacc caaccagtaa ataagtaaag
      481 acaattttgt gctgcgttga gaagtgaaaa ataaaccttt gtagtaatac tagttaataa
      541 aaaatataca aaaacactgc gaaaatcatt cttgagtttc gcagtgtttt ttgcctaatg
      601 actcaagttt attgggtaat actttgattt tctttctaca aataatcagc gatcagttgt
      661 aattttttct ctagttttaa tggctctttg tcaataagga gaaaaacaag tcgggtcttt
      721 tgtctcggaa agcttcaacc aactcgtgaa taaaggttac gcctccttga aaggcgtgga
      781 aaaaccaagt aatcgatcaa tcaccaattt cgcatttggt agaacatgtt tggtgagctg
      841 aaagtaggtg gcgttcatgt ctgtaactaa gaattctatt tctttaggat tagtgcagct
      901 aaggaaatag tttgttaatc gaggaatttt ttgtgtaggt aaattttctc gattaatttt
      961 tctgtctcgc catccgcgga aataaaactc agtttatcct caacggaaac gtgagaacga
     1021 aattcacaac cctcaacact caagacagaa ttcttttgga agactttggt aaatagcttc
     1081 tcaactcttt caatgtgcga ataacggttg tcaaagatac ctaacaactt ttcgaaataa
     1141 aaaataacga cactttttca gtcagtaaag aagtgatttt atgtctgaca tgatttgaaa
     1201 tcgagtgcct tggacggata aaataacttt gagcgttcca atggcttcga cagtttttac
     1261 aggtatagcg ctgctttttt aggcgtataa ccattggcat atgattatat tattcaaagt
     1321 ggacaatggt ttccttcttc ttgtttttca ccaccattaa tttcctattt ccatctacca
     1381 cagcagaacc acaatttcta cagacacatg gagcagggaa gagaactgca gcaacgatca
     1441 atgttttttt cttctgtaag gtttcgtaag agacctctgt aatcatcaaa tttttgtcta
     1501 ttgatctcag aatttttttg atagaaccat tcataaagca tatcgttctc tcagttgttt
     1561 attttgtggt gatttaatca tacaagaaaa cgatatgttt ttttataaaa acatgaatag
     1621 gaaaaatagt tgacaagaaa atgacaacgc catatgatgt tccatagtat ttacaacctg
     1681 tcatagatga ctgtaaaaaa taaaagtaaa aagaaaggac gacaacaggt ggatttttat
     1741 cagtctctac aactagatcc gtttatttta aaacaaaaaa tacgagaagc agcttctaaa
     1801 aaagaaaagt gcatgtatat ttgttcattg tttttacgat cattattcat cgtattgttt
     1861 gccatttgtt ttatcattat aattacaaca ttatttgaat cgaagcataa accatacgct
     1921 gtggttttat tctgtatgtt gatgagtatt cgttttgttg actttggtta taagatttcc
     1981 cattcgatca taggtttagc aatcgtcatg ttctcactat tagtagcacc gtatgtccag
     2041 ctgatcaaat ggtcagttat gggcatgttg attcatttta ttttactaag cagcatatta
     2101 atggcaaccg ctagtgatcc caaaatgggg aatgcttctt tgtacggctt ttcatatctc
     2161 tttattgttt attcgttacc taaagattcg ttgaacaaga atttctttac acaaacaagt
     2221 agcttattat ttttgttttt ttgttggttt tcagttctcc tttacagaaa acatcgagaa
     2281 aagaacaaag acaaaagctt gtttaaagag atttttttga aaggcatgta ttcgcaggaa
     2341 aaaatttgga tgctcatcta tgcatttggc attagtcttt tgatcgttgc aggagagtat
     2401 gtcccttttc agcgattgat gtgggcaggg tttgcatttt cttctattgt atctagctat
     2461 ggattgatgt cgattggttt caaagaacga gcagtagacc gaatcattgg ctcattgatt
     2521 ggatgtgttt tatttattgg tatcagccaa tttattcctt ttaactgggt cggtatatta
     2581 ggtggtctag ctttaggtgt ttgttctacc tatagatata aaacagtttt taattctttt
     2641 ggtgctttgg caatcactgc cagtttattc ggggttccag gagcagtaac gatccgcatt
     2701 tttgagaata tccttggggt atgtttagga atcatgtata ttggggtgac tgaaatactt
     2761 atccgaaaaa taagagaaaa acacggtttg aatcattaga tgagatcgaa taaaaaataa
     2821 ggaaaaagtg aagatacaca tggatgataa attgctatca ttccatgtgt atctttttac
     2881 ggtaaatcag tgaaacgttc gactttcatt tttaatttat actttttacg gagttcgttt
     2941 atttgagcca tcatttcgga tttgtgatgc ttttcgattg cttcttcatc tttccaacgg
     3001 tcgatcagta aaagagattc tggatcttct agtggaaagt aatagtcata tttctcgttt
     3061 ccttcttctg cacgaatcgc tttgacaatg tttctttcga ccatttcttc tgcaaatttt
     3121 ctggcttgtc catttgtact tgtatacaaa atattcactg tgatactcac taaatcccct
     3181 cctgaaaaaa ttctatcact agtatatcac ggtgccagaa attattattc aagatatgat
     3241 acacattttt tccgcaatgc tacggaaata tatattttga atagaaaacg cttacaagat
     3301 atgcttggtt tataaaaata aaaggagtga ttgatttatg aaaaaagcat tgattatttg
     3361 tgcggcagga atgtcatcct cgatgatggc ctcaaagaca acggatttct ttaagcaaaa
     3421 aagagaagag gtactcgtag atgcagtttc agctacagaa ggcgataata tgattaagaa
     3481 tagtgatttt gatttatttc tgatcagccc gcaaacgacg atgtttttgg ataaattcaa
     3541 aaaattagga gaatctgttg gaaagcctgt tgttagtatc cctttccaag cgtatgtccc
     3601 aatccctaca ggcatccaga agttagcaga ggtgatcgaa gagaacatat gatggaatag
     3661 gagtggatga taatgaaaca gaaggaaaaa ttgctgttga gatatttgat tgatcacaag
     3721 aaggagtatg tcaccagtca acgtttagct tctgaattgt tattatctga tcggacaatc
     3781 cgtaattact tgcagcggat caaagagtta gtagaaaaaa acggaggtaa gatcattgct
     3841 aaaccaggtt atggctacca actgcatatt ttacaaagac tgacatttga cttgtttttg
     3901 agtcggcaag aaatagtacc tggatatgct agagaaccgc aaggatttta tgagtccgaa
     3961 gatcgacaaa agtatatatt gaaccaattg cttttggaag atgccattct ttttatggat
     4021 gatttggcag aagagcttta tatcagtcgt tccagtttga caaaggacat gcaagaaatc
     4081 aaggagcgat tgattccata ttcactaaaa atcgtttcta agcatggtca agggacatgg
     4141 atcgaagggg aagaaagaaa tcgacggcat tttatcatgg atactttttt tggaaaccat
     4201 tatggaaatt cattgaaaga atatttaggg caaagccgtt ttttcccaga catcagtttt
     4261 gaagagctag tcattatcat tctcgatgaa acaagagaag ctaagttaaa agtttcggat
     4321 tttatcatac agaatctgac tttacacttg gctttgggga tcaaacggct ccgagaaggg
     4381 tttgaaatca aggaattagg catcgaacaa gaagtttcta agcgagtaga gtatcaagtg
     4441 gcgcagcgga tcgttcaacg tattgaggta atcgcaggtg tatcgtttcc cgaagaagaa
     4501 attgcttatt tgacattaca tctcatggct aagtcgaatc acaaagaaaa tcaaaatgat
     4561 caggaactat caaaggaatt agcgaaagtt ctgaaaagat tgtctcagga atttcatttt
     4621 tcattagtag aggatctcca gcttttcaat ggactactcg atcatttgtc accgatgatg
     4681 attcgattga gccgaggtat tcgcattgag aacccattga ctgaggagat aaagaaagaa
     4741 aatcccaaag tctttgacgc agtaaaaagg catttcagca acatgccggc tttgaaaaac
     4801 tataccatca atgaagatga atgggcctat ttggctttgc atctcatggc ggcattggaa
     4861 aaagaaagag ctgcgcataa actacatgca ttgattatct gtgcaacggg ttacggcagt
     4921 gcccaactat taaagaatcg cgtagtcagt gaattcggta aaaatattac agtggtaagt
     4981 gtaaaaggct attatgagat caatgaaaca actttaaaag gaatcgacct gattatttct
     5041 tctattgatc tttcgacaat gtttttcaaa attccagttt tacatgtcag tatttttttg
     5101 aatgaagatg atgttcgtaa aatacgaaag gtagttggag aatctatacc ctatcattcc
     5161 tcagatagac cgataccagc attttctcta cagcaaaaaa agcagattta caccgaacag
     5221 ttgtctgaaa aatttttcaa gacttatacg tacgaaccga aaaaggaaga aattcttcat
     5281 gatttgctgc aaaagctgag cataaatgaa gaagaaggat atataagaga aatgcgaaag
     5341 cagatccagc aacgtgaaaa tatgggacaa atcatattca gtgatacagt ggcagtccca
     5401 catcccgctg ttccagttgg aatcgaaaca aaggtagccg ttgcattgat tccttctggg
     5461 atgcagtggg atcaatatca agaaattcat tttgtttttt tagtttcgcc ttcttatgtc
     5521 gaaaatgaag gaattacggt cgtgactaaa gcaatcgtta ggttagttga ccagctaaaa
     5581 gtacagcagg aaatcttagc tgaacctatt tttgagaatt tcagcagtaa atttatcaaa
     5641 ctgatcgaat aaggaggaaa acagatggct gaaaaaataa acagtgaaac actacaagtg
     5701 acttcttttg atatcatttt ccatagcgga aatgcacgga cactgataca tgaagcattt
     5761 cgctcgatgc gtaaagagat gtttgaagaa gtagaagaga agttgcgaca agcaaatgaa
     5821 gagattcttc atgcgcatca gtcacaaact caacttctga aagaatatgc cagcgggaca
     5881 aaaatagaaa tggaaattat catggtccat gcacaagatc atctgatgac gacgatgaca
     5941 ttattggaag ttgcacagga aatgaattat ttgtatcaaa aaaataaata gaaaggaagg
     6001 atcattgtgc aggatacagt aaaaaggaaa atcgaattag aaaaaaagca acttagtttg
     6061 aaaaacatgt atttcaatcg ttatctaatc gtacgttacc tgactgcctt tttctttttc
     6121 atgaacatgc aatggatgat tctgcttcta tcagctaaaa gtttaggaag tagtttaccg
     6181 atgattctga ttttagctat tttgcctgca gtaggagaac aagtaaagct ttatcgaaaa
     6241 caccaaacga acgttccatg gacaaagtgg tattttttgt tccagagtgt ctttaattta
     6301 ttagtgattc cagtgttgtt tacctcagga tttacattct tgtatccttt tatggcaaat
     6361 aacaatggag gacagttatt tgttttcatt cttgttgtaa gcggtatttt tgccagtgca
     6421 tttatacagt atcgtttaaa gaaaatttca ctcaaccaag accaacagta tatccgtatt
     6481 aaacaatatg aaaaagcgtt atatttagga aaggagaata actaatgttt gattttttgc
     6541 agaaatacct gatgggacct atggggaaaa ttgcgcaact taaaatcgtt cgggctgtca
     6601 tggcagcggg gatggcttct attcctttca cgattgtagg ctcgatgttt ttggttttaa
     6661 atattttgcc attaccgttc ccatttttag aaggattttt taatgcaact tttttcaaag
     6721 ttagtgactt gtacatgatc gtcaatacaa tgacgatggg gattttatct atttactttg
     6781 ccattgtgtt tgcttatgaa ttaacgtcaa ttgaacgaga tgaacaaaaa ttaaatgtta
     6841 atccgctcac gggggcatta ctatctgttt ttgcattctt tatgtgtatc cctgaattga
     6901 tcatgactga tggtaaaatc agcttgattg cttctatcaa tgagacagaa actgtagtaa
     6961 atggtattag gatgggctct tttgtcgaac gtttaggaac ttcaggaatc tttacagcta
     7021 ttattatgtc gattattgcg gtcgaattgt attgtatgtg tgtcaagaga aattgggtca
     7081 taaaaatgcc agatgttgtt ccgccaggag tttctcgttc tttcactgct ctgatcccaa
     7141 catttgtcat tgcgtttgtg gtgatgatca tcaatggatt attagttgca ctaggaacgg
     7201 atatcttcaa aatgattgct atcccatttg ggtttgttac gcagttgacg aatacatggc
     7261 ttggtgtcat ggtgatttat ttcttgatcc atgcattgtg gatcgtaggg atccatggag
     7321 cgaatatcat cacttctttc ttgaccccga tcgtattagc taatatggca gctaacgcac
     7381 aaggcgcaaa ttatccgttg gcaggagaat tcaacaattc ttatgtaacc gttggtggat
     7441 caggtgcgac tttaggattg atcatcttta ttgcattcct cgcgaaatct gatcagctga
     7501 aagtgttagg aaaagcctca cttgtaccag gattattcaa tatcaatgaa ccaatcattt
     7561 tcgggatgcc aatcgtctac aatccctatt tagcggttcc gtttttctta gctccgatgg
     7621 cttctgctac actcgcttat tttgccattc ggctggaaat cgtcaaaccg atgcttgcac
     7681 agatgccgtg gccttcacca gtcgggatcg gagcatttgt tggtagtggt ggtgattgga
     7741 aagctgctgt gttagcagtc atttgtgcaa tcatcgcatt cattatctgg ttcccattta
     7801 tcaaattcta tgataataaa ttattaaatg aagaaagaga aaaggcagca gaattagcta
     7861 gtgaaaagtt aaaatgattt ctaagaaaaa aggaactgtg acaaaaaatc acagtccctt
     7921 tttttcttag aaatgggaaa ccactagtat ataaatacca ttattcttca atcaaccgct
     7981 tcacgattgc ataatcccct tcataaggtt catctacacc attgattttt tgatagggat
     8041 ccacgccttt tcccgcaata acgactgaat cttgcggagc gctttctgct aaagcgcgtc
     8101 gaatcgcttc aggacgatcc atttcataat gaacgatcaa atctggatta gtaattgctt
     8161 cattgatttc ttccgcgatt tttttcggat cttcaaatgc tgggtcatct gccgtcaaaa
     8221 aggcgatgtc agctgtttct gatagcactt ttccaaaatc atgtcgacgg gagattgctt
     8281 tgtttcctgg gctgcctaat acaacaatta cacggccatc tggatgttca tttttggcaa
     8341 atttcagcaa tgttttcaag cttagataat tatgtgcata gtccacatag acatgcgctc
     8401 cattggattg gatcaattga tccattcggc ctggtacacg agcttctttt aatccgtctt
     8461 gtgcatcttg actggaggca cctgttagtg ctgcggcaat gatcgcactg gctgcatttt
     8521 cttggttgaa tcctccagcc agcaaaatat cataagcagt atcgcagata gctaagcgat
     8581 cttcatgaga agataaagta aaaccgtgag tacctttatc tgaacggatc acttgataat
     8641 tgctttcttc cgaacgacca taagtgatcg ttggaatctg gaatagatcg gctgtttccc
     8701 taagtaaatc aaaatagtca ctttcatgat ttaaaacaac tgtttttgaa ttcaagatca
     8761 attgtcgctt gcaatagaaa tagtcatcaa atgtcggatg ttcgatcgga ctgatatggt
     8821 caggcgagat attcaagaag attccgacat ctaaagtcaa gccgtatacg cgttgtgttt
     8881 tgtaagcttg tgaagaaact tccatcacta gatgagtcat accattttca actgcttctg
     8941 acatcatacg atataaatct aatgactcag gcgttgtcaa atgggatttg aaatatgttt
     9001 tgccatctaa cgttgtattc atagtagaaa gcaatgctgt ttttttattt gttgtgtgat
     9061 ccaagattgc ttttgtaaag tacgcagcag ttgttttacc ttttgttcca gtgaatccga
     9121 tcactttcaa tttattttgc ggatagtcgt aaaaagccat agcaagaagc gccattgctt
     9181 tacggatatc cgttacgatg attcctactg ctgaattttc gtatggttgt tctgatacat
     9241 aatattgaat accctgttcg attgctgaat ccaaatattc ttctttgaaa ttcattcctt
     9301 tacagaaaaa caaagtagaa gcatcagctt ttcttgaatc ataacttaac tgaagaaatt
     9361 cagctttttc tggtacatca agatgccagc ctcttgttga aacaaattct tttaatagat
     9421 tttcttttaa tagtaaatga cggatgtctt ctaaagagaa gctcattgat tgatccctcg
     9481 ctttattcaa tttcttggtt tattatagca cactcttttt aatatatcgt ttgtttccgc
     9541 tatgacaaaa tccagcttta ttcaaatttt cttgacacag gggacaagcg gatgagtatt
     9601 ttcataaaca atgaactgac tgtaatagat aggagaacga atagtgtctc caacaatcca
     9661 aaaacataat aattcaaaaa gacgataaga ctattggtaa gtaaaagtcc ttgcggcatt
     9721 ggaagccgaa aaaatttgtg gaaaataatc ggcggtatcg ttataccgcc atttgaggcc
     9781 ccgactgtgt acaatgtgta aaccccaaga ctgaacaaaa gacttcctga aatcagtgaa
     9841 aagaaatgtg aggagatcat ttccactttt ggaacaatct ctaaaacaac aggaaaaagc
     9901 agacttcccg ctacagtctt aaaaaatact gattttccta ataggacagc ggctagaagc
     9961 agcatcataa gattgatgct ccaaacagta actgagcgtt ggatagaaaa ggccgtttca
    10021 actaaatgtc cgatggcgcc cactccggcg gaagctatat cgtgagggag caagaaccaa
    10081 ttcaatccaa ctgtgataaa gaaaagacct aaaaatataa cgggtagttt ttttatttct
    10141 tgtgtcattg atatctccta taaatgttag ccataaaagg ctgtatattt ctttacttta
    10201 ccaaaaaaac gaaaccgaat tcaaaaaaac aattgttctt ataaaagtta tataaaatcg
    10261 attgccagtt tctgttttac caattaaaat gatagtaatg ttttgtagaa agttggaatc
    10321 tgaccgatgg ataatcaaag aaaaccagca ttagatcaat taacaccgca agaaaaaatg
    10381 gcacgaggct ctgcttggat gaccgccagt aatatcattt caagattgct aggcgctgtc
    10441 tatatcattc cctggtatgc ttggatgggc gaaaatgcta aggctgccaa tggattattc
    10501 aatatgggat acaatatata tgcattgttt ctactgatct ctacagcagg aatacctgct
    10561 gcgattgcaa aacaaactgc acgctataat tcattgaatg aatatggcac ttcacgacga
    10621 ttatttatcc gtgcattaca gatgatgggc gggttaggcc tgttgtttgc tttgttcatg
    10681 tacatcgcct ctccttggct agctcatgct tctggtggcg gagaagaact gattccaacg
    10741 atgcgttctt tgagtatcgc tgtattggtt tttccttgta tgagtgtgat acgagggtat
    10801 ttgcaaggca atcaagaaat gatgccttat gcgctgtcgc agattgtaga acaggtggca
    10861 cgtatttttt atatgctatt ggctacattc atcatcatga aagtcatgga tggtgactat
    10921 gtgacggcgg tcacgcaatc aactttcgcg gcatttatcg gtatgctagc aagtattctt
    10981 gtccttttgt attttttgaa gaaacatcaa gcgtatacat cggcgtttat tcactacagc
    11041 gaaaacaaaa taaatattgc aacgaaggaa ttgttgttag ataccgttaa agaagcgatt
    11101 ccgtttatta ttgtgggttc aggggtaacg atcttcaaat tagtcgatca gttcactttt
    11161 atgaagatca tgtctgcatc caccgagtat tccaatgcac agttactgga tcttttttcg
    11221 atttttagtg ccaatccaga taagttgacg atggtagtta ttgcattggc aacatcgatc
    11281 gcgtctactg ggttaccgtt gattacagaa gctgttacgt tgaaagatcg agtaggtctg
    11341 gcaaaattga caagcggaaa tttacagttg ttttcatttt ttatgtttcc ggcgacattt
    11401 ggtgttatgt tacttgccta tccgctaaat acattatttt atacaccgga ttctctagga
    11461 agtaatgtac tgatccaagc aagttttgta ggactcttcc taggactata tatgctagtt
    11521 tccaacatgc tgcaaggaat gtttgaaaac aaggcggcaa ttaattattt ggttattggg
    11581 tttctagtga aactccttct tcaataccca gccattcgtt tatttgaagt atacggccct
    11641 ttattggcga caatgatcgg atttgctgtg tcttgtacct tgatcttaaa gagaatccat
    11701 gaagtagccc gctttaaacc aagttttgtc tggcgcagga cattgctgat ctttattctt
    11761 accttgatta tgcttttggc ttcgtggatc acaaagatga tttttggtgc ttttttaaac
    11821 gaagatagca aattccagtc tctattgttg attcttgtag tagcaggtat aggggcttta
    11881 gtgtatggtt atttagcttt gaaaatccgc ttggcagaaa gactactagg accaggaata
    11941 gcgcgtttac gacataaatt aaaaataaaa taagtgaatc agaagagcta gattgatgct
    12001 cttttgaaat gggggaaacg catgcgttta gataagtttt tagcagatat gggacttggc
    12061 agccgcaaag aagtcaaagg attaatcaaa aaaggaacga tcgaagtcaa tggacaagtg
    12121 gtgaaaagcg ataaagcgca agtaaatgaa ttcgaagatg cggttactta tttaggagaa
    12181 atcattacgt atcaaaaaga tttttactac attttgcata aacctgcagg agtcatttct
    12241 gctactcagg ataatcatga tcaaaccgta atcgatttat tggaagacca ggattatcgg
    12301 gaagatcttt ttccagtcgg ccgattagat aaagacacag agggcctctt gattttaacg
    12361 aatgacgggg tgtttgccca tcagttacta tcacctaaaa aacatgtgga gaaagaatat
    12421 ctagctgagg ttagcggtat aatgacagaa gaagatatcc gtttatttgc agaaggactg
    12481 attattgatg gcgatgagca gacattgccg gcaaaactcc aaattgatca agtagatcaa
    12541 gagacagaaa cttcaattat ccgtctgatt ttgcatgaag gaaagtttca ccaagttaag
    12601 cgaatggtca aagcagtagg aaaagaagtc ttatatttaa aaagaatccg tatgggaaaa
    12661 ttttggctgc ctaaagattt gaataaaggg caatatcgac ccatgaccaa agaagagcta
    12721 gaacaagtga gagaaaaata attgaatgga tagctaaaag aataagcaga gtagttgtag
    12781 cgccatctga aaagcagact tgtccgtctg actttcggat ggcttttttg tgaaatttcg
    12841 gcttatttcc gtagaagcta cttctggaac accgtttgtt cggaataagg gactgtgaca
    12901 agacttttgt tacagtcccc attgttcctt gtgactcaga gttagatagt aaggacacga
    12961 tctccctaat actcaggcca aaacgctgaa ttcacaacct ctgataaacg gtgttcaatc
    13021 agctgaccct cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttcccgtttt
    13081 tttctctaat actcaggcca aaacgctgat ttcacaacct ctcctatact ttgtttcatt
    13141 ttccctatac tttgtttatt tattctcatt attgaaagcg ctataattac gttgcgaatg
    13201 aatcgaaagg ggtttttaga tgaaaaaacg aaaggggttc aatcattttt ttatgaatct
    13261 atggcgttat aaagcacttg tcctgatggc tgtaccgggt atgctttgga tgattttttt
    13321 cttttacata cctgtaatgg cgaatgtcgt agcattcaaa aattttcata tttcagcaga
    13381 tgggttcttg gctagcttga gggaaagtcc ttgggtgggt tgggaaaatt tcaaattttt
    13441 attttcttcg gatcaagcat ttctcatcac taaaaataca attttatata atgtagcatt
    13501 tatccttttg aatctgctga tctcagtcgt atttgcaatc attatgagcg agttgagaaa
    13561 taaacgactc gttaaagttt atcaaacgat gtccttacta ccttattttc tatcttgggt
    13621 cattatcagt tactttgttt acgctttttt aagtcctgac aaaggaatat tcaatcagtg
    13681 gatcactgga catggaggag aagctgtcaa ttggtataat gaaccgaaat attggccgtt
    13741 tatcttggtg ttcattggta catggaaagg gattggttat aacagcatta tttactttgc
    13801 atctgttatg ggaatcgatc cgacttatta tgaagctgct atggttgatg gagctagcaa
    13861 atggcagcag atcaaacatg tgacgatacc acaacttatt ccactaatga cgattttaac
    13921 gattttagcc gtaggaaaca ttttccgagc tgattttggc ctgttttata acattcctag
    13981 gaattccggt gcattatatg atgtgactca agttttggat acgtatatct acaatggcct
    14041 gacatccaca ggagattttg gtatgacggc tgctgcagga ctttaccaat cagttgtcgg
    14101 ctttgtcttg ttgatgatca caaatacgat cgcccgacgt tttgatagcg attctgcatt
    14161 attctaagga ggaaaaaaat gaaaaaaaag aaagtggaga gagtccaagt ccgctctttc
    14221 aataagacgg ctaatttttt ctctaatata atgattgcac tctttgctat ttcctgtatt
    14281 ttgccattta tatttgttat cgccatctct tttaccgatg agtccatatt gtcgactcac
    14341 gggtacagtt tttggccaga aaagttcagt atatttggtt atacgtactt gttcgaccag
    14401 atgcaggata aaattttcca agcgcttttc gtcactgtac ttgttactgt tttaggtaca
    14461 ctgatcaata gcacggctac ttctttatat gcttatgcga tttcacggtc aaactttcca
    14521 ttccgtcgtt tttttaccgt tttttgtttg attacgatgt tgttctcacc agggatggtg
    14581 gccaattatc ttgttatgac aaatcttctt cagttaaaag atacgatttg ggcattgatc
    14641 ttgccaatgg ctgtcagtcc cttcaatatt attgtgatgc gcacattctt taaacgttcg
    14701 gtctctgatt cgattattga atcggcacgg atcgatggag ctagcgaact gcgaatattt
    14761 gttcagatcg ttttaccgtt agctgttcca ggtattgcga caatcagttt atttgccgca
    14821 ttgggttact ggaatgactg gttcaatgct ttactataca tccaagatga taagctagtt
    14881 ccactacagt atcttttgat gaaaatccaa agtaatattc aatatctgac acaaaattca
    14941 ggagcaggaa gccaattgac aggcggatta gcctcagttc caggagaatc tgccagaatg
    15001 gcgatcgtag tcatctcgac gttgcctatt gcgatcagct atccattttt ccagaagcat
    15061 tttgtcaaag gtctaacaat cggcggagta aaagaataaa gaaacaaaaa tataaatcat
    15121 aaaattagga ggaagaaaaa tgaagttatg gaagaaacta gcctttacag gtgtttcagc
    15181 gttgttgttg ggaacattag cagcatgcgg aggttctggt tctaaagacc aggcagaagc
    15241 tagcaattcg gacactttgc agatgtatca aatcggagat aaaccagata atttcgatca
    15301 attgatggaa gtagcaaaca aacgaatcga aaaagagatc ggtgtaaaag tcaatatcaa
    15361 ttacattggc tggggcgatt acgaaaagaa aatgaacgtc atcatttctt ccggggaaaa
    15421 ttatgatatc gcctttgcga acaactatgt ttccaatgcc caaaaaggag cctttgctga
    15481 tttgactgaa ttagcgccta agtacgctga aaaagcttat aatgatttag atgaagcata
    15541 tattaaagga aatctggtca atggaaaatt atatgctttc ccagtcaatg gaaatgtctt
    15601 tgctcaacaa gtacttactt ttaacaaagc tttactagat aaatacgatc tttcaattga
    15661 tggaatccaa tcttacgctg atgcagaaaa agtcttgaag gaattccatg aaaaagaacc
    15721 aaacacggca gcttttgcca ttggacaagg cttcaaagtc caagggaact ttgattatcc
    15781 tttagggaat acgttaccat ttgctattga tctggatgga gatgattcga aaatcatcaa
    15841 ccaatacgat aatgaaacgt atatcggact gttacgtaca atgcatagct ggtacaaaca
    15901 aggactcatt ccaagtgacg ctgcaacgag caataaggat tatccactag aaggaaatac
    15961 ttggctaatg cgtggagaaa cacaaggacc gtatgattat ggcgatacaa ttttgacaaa
    16021 tgcagcaaaa caagaacttg tctcaaaagc aattactgtt ccactaaaat ctacagctca
    16081 agcacaaatg gcaaattttg tcgtatcaaa tacttcaaaa aataaagaaa aatcagtaga
    16141 acttctaggg ctgcttaaca gtgatccaga attattgaat gggcttgttt gggggatcga
    16201 gggagaagca tgggaaaaag tcgatggttc agatgataaa atcaaattgc tagatggtta
    16261 tcagccaaat acacatatgt ctgcttggaa tacaggaaac aacaagattc tatatacaca
    16321 agagtctatt acggatgaca tgattgccaa acgtgatcaa tcgattgctg atgcagaaac
    16381 ttcaccgatt ttaggattca gcttcaacac tgacagtgtc aaaacagaat taagtaatat
    16441 ttctaatgtc atgaatcaat atttagatgg attgaataca gggactgttg atccagatga
    16501 aacattaccg aaattgaaag acgctttgga caaggctggc tatgataagg tattgaaaga
    16561 aatgcaaaaa caatatgatg agttccgaca agaataataa tgaataaaga caaaggggtt
    16621 gtgacaaaag acgtgtcaca acctcttgtt ccgaataaac ggtgttcaaa agccagtttc
    16681 tcggtattaa gctaaatata tgtaaaaatg tggaaagcca tattcacaaa atttttctta
    16741 ctactcgaaa ctaagcggct atttcacaaa ctcttggtaa acggtgttca atcagctaac
    16801 cctcggtatt cgctcacaaa acgacaaaat atgaggaact atttttccgt ttgtttctct
    16861 aataatcagg tcaaggtgct gattgcccaa cctttttttg tataatgaga aagtataaac
    16921 ttaatgtttg gaggagaaga tatgttaggg aaggttttgg aagagttatt tatccggatc
    16981 tgggtcatta taaaactaac cttgtatttc tggatatata catttgccgg cgggatcatc
    17041 tttggattag gtgcggcatg gaaaacagtc aatgaactat tttatttgta cggattcgag
    17101 tataaagaaa tcacaataaa aagaggctgg aatatatata aaagaaattt tttaagaggc
    17161 aatctgctat ttagtttatt tttatctggt actgctttat tgagttacaa tttatattta
    17221 tctgttcaaa tcaaagggct cattttttta gctattgatt ttattttact ttttgccttg
    17281 ctctgcctat ttgctactta tcaatacagt atgattttag atagtgaata tacgatctct
    17341 gttcctaatc tgctaaagct aagttttatt tcggtttttt ccagcttttc gtcttttcta
    17401 aaaataatta ttggtagcgg tataatttta ggcgtgactt ggcaattcaa aggattgatc
    17461 ctgtttggtg tcattggtct tttgacggtt tggaatggta cgatgacaac acattggcgt
    17521 gaagaattag ataaacagtt ggaaagttat gaataagttt cggattctat tttcaaaagg
    17581 aaccttacta aatcgtctgt tgcggaaata cgcggttatc atgatcggtg taacgatgac
    17641 tgctaccgtt atttttagta tccatacttg ggaacaaaat caaaaacagg cagaaaacat
    17701 gacaagtgat gccgttcaaa gcacaagcag aatgctgaat gataaaacaa ccttgagtag
    17761 gatcatcaaa aaccagcttg tagggaattc cgaaaagatc gaaaatgtga cgacctactt
    17821 aacaaaaccc atagatcagt atctgatgta tgtatacgaa cagcaaaaca gtaccgatga
    17881 attagtttct ttcccaaatc aaatcaaaga tctctacgca aattatgaag aactaagtgc
    17941 gatatatatc gtgttgaatc aactaccgga gtattatgaa tcttctagag aaaacaaagg
    18001 tggggaaata aaaaagggga caccaaaatt actagacgcc ttttatatca agttgcctat
    18061 cacacaagga ggagctgagt caggaagtat ttttattggt tttcaaaaga aagaactaga
    18121 tatgatttta gaaaatctaa cgtcattcaa tggactctct ttatacatga tttctggaac
    18181 gacaaatcga ctatacacat tccatgataa taaaatagct aaagaaacgt ttgaaagaca
    18241 agaaatgatc atcacagaga gtctgaagaa aaattcacaa ttgccgattg acgatctgga
    18301 aaaaaacaat tttgttcaac accaagaact gtctgatgat tatcgtatat tagctgtact
    18361 agataaaaaa agtgtaaaaa tcgaaacggg aaaaaatctt gccccgctga tcattggtat
    18421 cattatttta gacaatatac tgcttatctt tctttataaa acattcaaac gatattcaca
    18481 gcaagtatct atcgtcatgc aggccatgga tcaaacagca gcagggaatt tagagacgcg
    18541 gatcgacata acaaacacgg aatatgaact gagggaactt tcaatcggta tcaacgaaat
    18601 gttggatagc atcaatcagt ttgtagaaga catctacaaa ttagaaatca aacagcaaga
    18661 cgcacatatg cgggcgcttc aagcacagat caatccacac tttttatata atacattaga
    18721 atatatccga atgtatgcaa tcagcgaagg aagtgaagaa ttagcagatg ttgtgtatgc
    18781 gttttccgct ttgcttcgaa acaatacgaa tcaagagaaa acgatcactt taaaagaaga
    18841 actggacttt tgcgaaaaat atgtctattt gtatcaaatg cgttatccta atcgagtagc
    18901 ctatcatttt atgatcgatc ctgatttgga aaagatcgaa gtacctaaat ttgtgatcca
    18961 gccattagtt gaaaattatt ttaaacacgg tattgatttt actcgattcg ataatgcgct
    19021 aagtgtcaaa gtcttgcagg aaggcaaacg ggtaaggatc atcatcaaag ataatggaaa
    19081 agggatgact gaaaaacggc taaaacagat tgaagaaaaa ctgtctcatc ctaaagtgga
    19141 acttcacggt tctatcggtc tgcaaaacgt gaatgaacgt ttacgtgcca gcttcggcag
    19201 cagttattat atgtcgcttg agaataatga gacaggtgga ctgaccgttt ctattacatt
    19261 caaggaggga tgagcgtgta tcaagtgtta ttggtagatg atgaatacat gattttgaat
    19321 gggttaaaaa agataattga ttggcaaagt ttaggctttc aaatagtagc aacagcagag
    19381 aatgccaaag aaggactagc tgtacttgaa cagcgacaga ttgatttagt agtaacggat
    19441 gtgacaatgc cagaaataaa tggactggaa tttattgaag cggctcaaaa agaacgtcat
    19501 aattttgaat ttatgatttt atcaggatac caagaattcg attatttgaa aggaggtatg
    19561 caattaggcg ccgtgaatta tttgatgaaa cccgtgaata agttcgaatt ggttgaaagc
    19621 ctcaagaaaa tcaaaacacg gttagatcag caaaatgaac aaaaaaatca acaagaaatc
    19681 tatcaagaac tgttattcag tcaatggttg aatgaagaat tggatgaaat aagtgaagaa
    19741 gaaataatag gacaaataag tgagaagcag cataggatag tattgattca actggcaaga
    19801 actcatgata ctcttatcaa tagatggttg caagaacggc aacaaatctt ttactatcaa
    19861 cgaaattacg gagaccgtgt attgatcacg ttgttgttgg aggcagatga agttgatggc
    19921 ttctgttgtt ttgttcaaga aaactttcct gatcaggaat ggctgatcag tatcggggaa
    19981 gagacagatt ctttggacaa aatccctgaa acattccaac aagcgaaaga caatctacag
    20041 ttgcatcaat tttatggaga taaagacgag catgtttttt acgcagtaca agcagaaaca
    20101 aaagatcaaa ccatcgattt ttcaagtttc agacgcgtgc tgcaaaataa acaattggaa
    20161 gtcgctcaaa aaatgatcac tgattttttt gaacagtttc agttagcagc catgatgccg
    20221 gaagatatac gatattccag ttttttactg tttatggaga ttcaacggga attgattgat
    20281 ctggaagatg aggaatattt acaaggaatc gaaaaaatcc atcaggcaaa aacggtgcaa
    20341 gaacttcagc agctacttct gtcattcatc aaaaagcatc agagacagaa aaagtacagt
    20401 gacaatgtgg aaaaagtaat cgtaatcctg caccagcatt atcaagaacc acttacactc
    20461 aaagaagtgt cagaaaacct gcatttaaat gtcatgtatc taggacagct gttcaaaaaa
    20521 gaaacgaaaa aaagtttttc agcttatttg aaccatttac gtatggaaaa agcgaaacaa
    20581 ctgcttttgc acagtaatca aaacatcaat gagatagcaa gtgagattgg ctacaacaac
    20641 accacgtatt tctctaaatt attcaagaaa atcgtcggca gatcccctaa agagtaccga
    20701 gaaaacagtg gagaatcata aaagaaaaag ccaatgtaat gaaataactt ataaaaaatt
    20761 gtctagaaat ctaattttgg gctgttctta ttacttgagg caaggcagct ttttcatgac
    20821 ctctcgataa acggagttca aaagccagtt tctcggtatt aagccaaatt tatgcaaaaa
    20881 tgtggaaagc cattttcaca aaatttttct taatactcga aactaaacag ctttttcacg
    20941 acctctcc
//
LOCUS       DACDXE010000052        20863 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.052, whole genome shotgun sequence.
ACCESSION   DACDXE010000052 DACDXE010000000
VERSION     DACDXE010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.052"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            99..293
                     /locus_tag="IWM23_001886"
     CDS             99..293
                     /locus_tag="IWM23_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884517.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="IWM23_001887"
     CDS             562..861
                     /locus_tag="IWM23_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884518.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="IWM23_001888"
     CDS             complement(958..1392)
                     /locus_tag="IWM23_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9884519.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="IWM23_001889"
     CDS             complement(1624..2334)
                     /locus_tag="IWM23_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAP9884520.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="IWM23_001890"
     CDS             complement(2324..3184)
                     /locus_tag="IWM23_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAP9884521.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="IWM23_001891"
     CDS             complement(3189..3827)
                     /locus_tag="IWM23_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAP9884522.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="IWM23_001892"
     CDS             complement(3842..4141)
                     /locus_tag="IWM23_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9884523.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="IWM23_001893"
     CDS             complement(4184..5668)
                     /locus_tag="IWM23_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAP9884524.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="IWM23_001894"
     CDS             complement(5688..6149)
                     /locus_tag="IWM23_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9884525.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWM23_001895"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="IWM23_001895"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAP9884526.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="IWM23_001896"
     CDS             7452..8222
                     /locus_tag="IWM23_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9884527.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="IWM23_001897"
     CDS             complement(8495..9322)
                     /locus_tag="IWM23_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884528.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="IWM23_001898"
     CDS             9890..11125
                     /locus_tag="IWM23_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAP9884529.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="IWM23_001899"
     CDS             complement(12124..12384)
                     /locus_tag="IWM23_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAP9884530.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="IWM23_001900"
     CDS             complement(12411..13712)
                     /locus_tag="IWM23_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9884531.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="IWM23_001901"
     CDS             complement(14004..14462)
                     /locus_tag="IWM23_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9884532.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15800)
                     /locus_tag="IWM23_001902"
     CDS             complement(14475..15800)
                     /locus_tag="IWM23_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAP9884533.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17229)
                     /locus_tag="IWM23_001903"
     CDS             complement(15817..17229)
                     /locus_tag="IWM23_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884534.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="IWM23_001904"
     CDS             complement(17246..18010)
                     /locus_tag="IWM23_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAP9884535.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="IWM23_001905"
     CDS             complement(18114..18989)
                     /locus_tag="IWM23_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9884536.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="IWM23_001906"
     tRNA            complement(19244..19328)
                     /locus_tag="IWM23_001906"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="IWM23_001907"
     tRNA            complement(19346..19417)
                     /locus_tag="IWM23_001907"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="IWM23_001908"
     tRNA            complement(19427..19499)
                     /locus_tag="IWM23_001908"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="IWM23_001909"
     tRNA            complement(19561..19645)
                     /locus_tag="IWM23_001909"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="IWM23_001910"
     tRNA            complement(19666..19736)
                     /locus_tag="IWM23_001910"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
     gene            complement(19782..19853)
                     /locus_tag="IWM23_001911"
     tRNA            complement(19782..19853)
                     /locus_tag="IWM23_001911"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19819..19821),aa:Gln,seq:ttg)
     gene            complement(19865..19937)
                     /locus_tag="IWM23_001912"
     tRNA            complement(19865..19937)
                     /locus_tag="IWM23_001912"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19902..19904),aa:His,seq:gtg)
     gene            complement(19940..20013)
                     /locus_tag="IWM23_001913"
     tRNA            complement(19940..20013)
                     /locus_tag="IWM23_001913"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19979..19981),aa:Trp,seq:cca)
     gene            complement(20020..20100)
                     /locus_tag="IWM23_001914"
     tRNA            complement(20020..20100)
                     /locus_tag="IWM23_001914"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20064..20066),aa:Tyr,seq:gta)
     gene            complement(20102..20177)
                     /locus_tag="IWM23_001915"
     tRNA            complement(20102..20177)
                     /locus_tag="IWM23_001915"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20142..20144),aa:Phe,seq:gaa)
     gene            complement(20213..20285)
                     /locus_tag="IWM23_001916"
     tRNA            complement(20213..20285)
                     /locus_tag="IWM23_001916"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20250..20252),aa:Asp,seq:gtc)
     gene            complement(20295..20367)
                     /locus_tag="IWM23_001917"
     tRNA            complement(20295..20367)
                     /locus_tag="IWM23_001917"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20332..20334),aa:Val,seq:tac)
     gene            complement(20383..20454)
                     /locus_tag="IWM23_001918"
     tRNA            complement(20383..20454)
                     /locus_tag="IWM23_001918"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20419..20421),aa:Glu,seq:ttc)
     gene            complement(20469..20558)
                     /locus_tag="IWM23_001919"
     tRNA            complement(20469..20558)
                     /locus_tag="IWM23_001919"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20522..20524),aa:Ser,seq:gga)
     gene            complement(20598..20671)
                     /locus_tag="IWM23_001920"
     tRNA            complement(20598..20671)
                     /locus_tag="IWM23_001920"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20635..20637),aa:Asn,seq:gtt)
     gene            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="IWM23_001921"
     rRNA            complement(20681..20796)
                     /gene="rrf"
                     /locus_tag="IWM23_001921"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6572 a         4381 c         3323 g         6587 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatagaa actggggtag ctggattcga
    19801 accaacgcat gagggagtca aagtcccttg ccttaccgct tggctatacc ccaagaataa
    19861 aaagggcgaa tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac
    19921 cacttcgcca cattcgccat ggcaggggca gcaggaattg aacccacact aacggttttg
    19981 gagaccgctg ttctaccttt aaactatgcc cctataaaat ggaggagagt ggattcgaac
    20041 cactgaaccc taaggaacgg atttacagtc cgtcgcgttt agccacttcg ctactcctcc
    20101 atggtggcgc gggacagaat cgaactgccg acacacggag cttcaatccg ttgctctacc
    20161 aactgagcta ccgcgccata ttttaaaatt attaattgaa taacaagaac gacggttccg
    20221 acgggaatcg aacccgcgat ctcctgcgtg acaggcaggc atgttaaccc ctacaccacg
    20281 gaaccagttc attatggagg ttaacgggat cgaaccgctg accctctgct tgtaaggcag
    20341 atgctctccc agctgagcta aacctccaac attcaattaa aatgacccgt acgggattcg
    20401 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaacg ggccaggtaa
    20461 atttgctacg gagagtaagg gattcgaacc cttgagacag tttggcacca tctacatgat
    20521 ttccaatcat gctccttcgg cctctcggac aactctccag agtgaaagaa gctcaatact
    20581 ctttctcaaa atgagaactc cggcagtagg actcgaacct acgacatcat gattaacagt
    20641 catgcgctac taccaactga gctatgccgg aataacatca gcgtggcgac gtcctactct
    20701 cacaaggggc aacccctcac tacaatcggc gctaagaagc ttaacttctg tgttcggcat
    20761 ggttacaggt gtatccttct cgctatcgcc accacactgt ggtgttatct tttattgagt
    20821 aaattttgtt cactcaaaac tggatttgaa gtcacataag ttt
//
LOCUS       DACDXE010000053        20740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.053, whole genome shotgun sequence.
ACCESSION   DACDXE010000053 DACDXE010000000
VERSION     DACDXE010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.053"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..180
                     /locus_tag="IWM23_001922"
     CDS             <1..180
                     /locus_tag="IWM23_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884537.1"
                     /translation="LEEAGIEGIKNDRSSWSKLRYYFALYIDTTQLRNLAYTKLLFVD
                     CVKGLQKHLNELEQV"
     gene            342..644
                     /locus_tag="IWM23_001923"
     CDS             342..644
                     /locus_tag="IWM23_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884538.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            641..802
                     /locus_tag="IWM23_001924"
     CDS             641..802
                     /locus_tag="IWM23_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9884539.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            799..1104
                     /locus_tag="IWM23_001925"
     CDS             799..1104
                     /locus_tag="IWM23_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884540.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1104..1460
                     /locus_tag="IWM23_001926"
     CDS             1104..1460
                     /locus_tag="IWM23_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAP9884541.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            1420..1665
                     /locus_tag="IWM23_001927"
     CDS             1420..1665
                     /locus_tag="IWM23_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884542.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            1662..2081
                     /locus_tag="IWM23_001928"
     CDS             1662..2081
                     /locus_tag="IWM23_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9884543.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            2078..2635
                     /locus_tag="IWM23_001929"
     CDS             2078..2635
                     /locus_tag="IWM23_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9884544.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            2632..2928
                     /locus_tag="IWM23_001930"
     CDS             2632..2928
                     /locus_tag="IWM23_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884545.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            3005..3418
                     /locus_tag="IWM23_001931"
     CDS             3005..3418
                     /locus_tag="IWM23_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9884546.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            3764..4138
                     /locus_tag="IWM23_001932"
     CDS             3764..4138
                     /locus_tag="IWM23_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884547.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            4368..4712
                     /locus_tag="IWM23_001933"
     CDS             4368..4712
                     /locus_tag="IWM23_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9884548.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            4717..4998
                     /locus_tag="IWM23_001934"
     CDS             4717..4998
                     /locus_tag="IWM23_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884549.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            5101..5415
                     /locus_tag="IWM23_001935"
     CDS             5101..5415
                     /locus_tag="IWM23_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAP9884550.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            5393..7087
                     /locus_tag="IWM23_001936"
     CDS             5393..7087
                     /locus_tag="IWM23_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9884551.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            7107..8285
                     /locus_tag="IWM23_001937"
     CDS             7107..8285
                     /locus_tag="IWM23_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9884552.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            8248..8934
                     /locus_tag="IWM23_001938"
     CDS             8248..8934
                     /locus_tag="IWM23_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAP9884553.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            8934..10094
                     /locus_tag="IWM23_001939"
     CDS             8934..10094
                     /locus_tag="IWM23_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9884554.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            10104..10979
                     /locus_tag="IWM23_001940"
     CDS             10104..10979
                     /locus_tag="IWM23_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884555.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            10976..11287
                     /locus_tag="IWM23_001941"
     CDS             10976..11287
                     /locus_tag="IWM23_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884556.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            11277..11630
                     /locus_tag="IWM23_001942"
     CDS             11277..11630
                     /locus_tag="IWM23_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9884557.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            11620..12021
                     /locus_tag="IWM23_001943"
     CDS             11620..12021
                     /locus_tag="IWM23_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884558.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            12014..12418
                     /locus_tag="IWM23_001944"
     CDS             12014..12418
                     /locus_tag="IWM23_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884559.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            12430..13038
                     /locus_tag="IWM23_001945"
     CDS             12430..13038
                     /locus_tag="IWM23_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9884560.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            13058..13420
                     /locus_tag="IWM23_001946"
     CDS             13058..13420
                     /locus_tag="IWM23_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884561.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            13423..13605
                     /locus_tag="IWM23_001947"
     CDS             13423..13605
                     /locus_tag="IWM23_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884562.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            13622..17041
                     /locus_tag="IWM23_001948"
     CDS             13622..17041
                     /locus_tag="IWM23_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HAP9884563.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            17092..17829
                     /locus_tag="IWM23_001949"
     CDS             17092..17829
                     /locus_tag="IWM23_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAP9884564.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            17839..20130
                     /locus_tag="IWM23_001950"
     CDS             17839..20130
                     /locus_tag="IWM23_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884565.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            20154..>20740
                     /locus_tag="IWM23_001951"
     CDS             20154..>20740
                     /locus_tag="IWM23_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884566.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7430 a         3039 c         4456 g         5815 t
ORIGIN      
        1 ctcgaagaag ctggaattga aggaattaag aatgatcgtt cgagttggag taagctcaga
       61 tactatttcg cactttatat cgatacaaca caattaagaa atttagcata taccaaatta
      121 ctatttgttg attgtgttaa aggcttgcaa aaacatctta atgaacttga gcaggtgtaa
      181 tcagatggac ctaaagacat ttacagcaca gattgaacta atgcatcaag aagctttaag
      241 acaaagtgta tcgtacgaag acaagtggct caacacgttc catggcggac gtgagagcgc
      301 acttgatcaa gtactcaaat tactgaaagg agaatgtcgg gatggataag aaagcggcaa
      361 tgcagcgaat tatcgaattg acttattcag aagattggca aaatgacaaa gaagctgctt
      421 cagaagtgat gaggcttgga agagcgatgt gggcagacaa gagcaacaag ccaagaccac
      481 gaaaaatcgc aatttggcac ggtgacaaac ttctagtgat agggacagct gaacagttag
      541 caagtctcac aggcttgcac gagaaaatcg tgaggaaaag agcaaggtgt ggctacacag
      601 acgctaagaa gagaacgttt agatacgtgg aggaatcatc atgacaacag aagaagtgat
      661 tcaaatgcgt attcgaagcc ttcagcgtga gattgacgat ctggaacgaa caaaggcagt
      721 gatggtcaat gaaacggcga gaaaggcaat cgatttgcac atagagaatt taagaaggga
      781 aatccatcga ttggaggaat gagcgtggat aagaaagcag caatgaaaag aattgctgaa
      841 ttaaccaagt cagaatcttg gcaagaagac aaagaaatag ttgcagaagt ccaaaagctc
      901 ggtaaatcaa tgtggactga aaagcccaaa cggagaacgc cgagaaaaat tgcaatctgg
      961 catgatgacc gaattttagt aacaggtact gctgaacagt tatctgaaat tactggatta
     1021 agcaaaaaca ttatctggga tagagctagg agcttatgga ttgattcaaa aggacgacag
     1081 tttaggtatg tggaggagag ataatggatc tcattacaca atacagtgac atcatcctca
     1141 agaaaatcat gatgaagatt cagaaagata aaaaatcaaa agaacgagct gaattagtta
     1201 agttagaaat ggctgaaaca ggagcaggag tgcgaagtag caggcattgg aaagcagcag
     1261 caaacattga attttattac aacgaaattc aaaaagggtt cgatcagatg cgtgagctgg
     1321 atcggcaaac aaattggagc aagaaacttc atcaagatcg tttcaaattt gtagagaagt
     1381 ataaagaaat attagaagag tatttgagga ggacagcaaa tgataaaaaa actcgttcaa
     1441 ttcagcatgg atttatatga tatcgaatca ggagcaacac tatctgtgga atcggaccat
     1501 ctaatcataa attttggtgg aaagcgccag attattttgt gggtagttga tgatgtactg
     1561 tttccagaaa ttgttcatga tttcgaagaa tcaaaagcgg ttgagtttga aatagtgaaa
     1621 aaagtaatgg aattgattga aaaatacgag gaggacagcg aatgataccg aagtttagag
     1681 cgtgggataa acgaaagaac gtaatgagag atgtagccgt cttgcatttt actaaaaacg
     1741 gcaaaacaaa ctttattgaa tattggataa atcctaccga attgaaatca tatcacgtgc
     1801 gaaacatcga cctcatgcaa tccacaggaa tgaaagataa gaatggtgtg gatatatttg
     1861 aaggggatat agtattagtc agcgtgcgaa atggcttcga ttacttagat aataaagtct
     1921 gtattgtcaa aaattcaata gattattccg gattagtttg tgccactgtt gatgaagact
     1981 tagagtatca aatttttaac acagagctgt ttgaagaata cacgtacgaa gtcaccggaa
     2041 atatatacga gaatagcgag ttattggagg aacagcgatg aataaacagg aagcaatcaa
     2101 aaagttagaa agtattaaag cgataggaaa tgatgcaata gctgcttgct ataacgagag
     2161 tataaattca ggtattacgt taatgaaaaa aatagacgaa ccgcagaaac cagttattcc
     2221 acaactcgtg gccggttggc ttgagaaatc tacggaccct tttacaaaag ctgaaaaaat
     2281 agcgtattta atcaaatcta aagatggtga ttcatattat ttctgtgatt ggtttgtacg
     2341 agatggcata gtgacgcaag agcaaggaga agaattactc gcttgggcaa caagacaatc
     2401 atatgaaaca ctattgagcc tatacaacgg ctacgaggtt gagaaagagc cgttatgggc
     2461 aataaagaat gccgatggaa actatcttac taaatgtgct ttatggggaa aagatggagt
     2521 aaattatagt tttgaatgca atccatctca tcgattgctt ttcactgata aagcaacagc
     2581 ggatgctgca gcattgttgg tgaatggaac agtggaagag gtggtagaaa gatgaaacta
     2641 aaagacggat tttacgctag cagtcacggt atcggcggtt taatgctaga tatgccgaca
     2701 aagaacccta aaacacgcaa gaaatcaaaa ttcaaagtcg gtgacatggt ccgctgtgaa
     2761 gcagaagagt tcgtttatcc gttcagagga tacgttaaaa agatactgtc aaactcagca
     2821 atcattcgta ttgaaaatac aatgaagtgt gatcgttcga ctgcaaaaca caagcattat
     2881 ttagctgtag cgagattagt tgacatggaa gtaatcaaga gcaaataaaa aaagccggat
     2941 cgctccgact gattcaataa atccaacaca tttattatat cacataaagg agcggtttga
     3001 cttgatgcaa ttgttacgag aggtagattt caaacagaca agatgtaatg cgagagatgt
     3061 gctgaagaac tttcggcgtt tggagcggat ggcaggtcgc tctttgatag atattaagtc
     3121 gccgattatt acggatatgc cgaaggcacc aaagcacggc aataaggcag aagacgcgat
     3181 cattcagatg atggatatag aagcggagag agatgcgatt ctagcggctt tgatggctct
     3241 tagtctaatt agtcgccaga tactctacta cagcttctgt gtgccagata gcttctcaaa
     3301 ctacaggatt agccgtgaag tgggttattc agaaagaagt atacaacgga tgaagtcgga
     3361 agctctaata gagtttgcag aagcatataa acacggaaga ataattgctt ataaataatt
     3421 tggcggtttt ttggcggaat gatggcggtt tttagctatt taccagtgat attatggtag
     3481 tgtcgaaaga ttagtgatag gtctaagaca aaataaaatg taagggagga aatctccctc
     3541 atcgtttaat taagcttcga tagacagcag caaataaact aaaggatgtg ggggtcagct
     3601 cctacagtta gttcatgtgt tgctgtctat taattgcaac tctttcggtt ttattgagta
     3661 tttactcaac ttaaaaaaac tgttgtttgt agatgtaagc tgttcctatt tgtacatagt
     3721 aaaaaatgat ataataaaaa tattacgaaa ggggtgagag tgcatggaag acaaaatgta
     3781 tttaagacaa aaagttttca caaaaaaaga tattgaaaat caaattaatg gatttataca
     3841 gaaattagaa aatgcaaatt cttatttgat aaataaggaa ctaaataaag cttatgacca
     3901 gtggaaagaa gtatatgatg agttgaaatt gattcaaaat gaaacaaaat tagtacgaac
     3961 ggagaaaaag aatgaaaata gtttcttttt tgatggatat gctatattga tgttggaaac
     4021 tgtagcaaaa caaaatataa aagctcccaa aaaagaacta tctgacaata ttgataacgc
     4081 attagccgag ttaaggtatt acgttatgca aataaaagat gttaggatca ctcgttgagt
     4141 ggtcttttta ttttgcacaa aggaggtaac aacaatgtat agaccacaat acttagaaca
     4201 gaagtatgaa gtaatcactg tgcaaaatgg taacggagag atagtacgaa agtatagaag
     4261 accaataaag agcgatacat ataaacgaaa ggaaagcaat gaagttattc cattgtatgg
     4321 caaaagaata gctaagcatt aaataagatt gtgaaaggag acgggacatg aacgaggaat
     4381 tctatagatg gctattacag ttgataagag aagatcgttt ggttaagttc tatcagtctc
     4441 ctaaatggcg caggcttaga gagaaagcga tgaaacgaga tcactatgaa tgccaagagt
     4501 gtagaagact aggtaagtat catagagtag agaacgttca tcatataaag gaagtcaagg
     4561 atagacctga cttagcttta gatttagata atcttatttg tttatgtgtt gaacatcata
     4621 atgaagttca tggcagatat cttacagcat tggataaaca agagaagaag atagaaagct
     4681 ttgctaactt cgatgcaagt gaaaggtggt aagtgcatga tcatcaatga taatggcaga
     4741 gagtatgata cagaaaagat tgaagagtat tcatcttata ctcagggatt aattaaacgt
     4801 ttgatatacg ttcgctatgt aggtattagg gatctgttat cagataactg ttgtagtaaa
     4861 tacaaagtga atcaagtaag agaagcgttg aataaagata ataacgtcga aagaataaaa
     4921 aatgtttttg gatatagtat tgaagagatt aattattaca ttgacttcgc tgaagctttc
     4981 attccgatgg tgagataacc cccccttaaa ataaatcgca aattttttgg gggtgatgaa
     5041 acggaggggg ctgtcaggaa aagagatttt ttcgaacttt atcatgaaag gagggctaaa
     5101 atgtttaaaa acgaattgtc tcaaaatcgc tacagagaaa aattacgccg ctctttaata
     5161 agccaattgg aaagtcagaa aacaaatatt gagccattct tagataatgt tgatcgttat
     5221 atcagtttat gggaaacggc gatatcactg gaagaagata tatccgagaa cggcattaga
     5281 ctggagaatg gtaaaaagaa tgaatcagta gcgttgcttg tttctgtcaa caaacaaatg
     5341 ggattgatgt tggataaact tgccattact cctgaattgg taggtgaagc aaatgaatca
     5401 attcctgagt tataagcata ttgaaaattg gttcaaagct atagaagaag gcactatcaa
     5461 ggtatgcaaa gagcaattat tgctaaaaaa ttatctagaa gaaagagtct ttactagaga
     5521 agatatttac ttcgataagc agatggtaga ggattcaatc aatataccag cacaatactt
     5581 tccattcgaa ttaattccgt gggaaaaatt tctacaatgt tttatttatg gtgttcgatg
     5641 gaaaaaagat aaaacactag tgttcaatag atatctttca ttaatgggac gtggtaatgg
     5701 taaaactggt tttgcttctt ggaacaactt ctttctacta accgctaaac acggtattaa
     5761 aaattatgat attgatatct atgccaataa tgaaagccaa gcaaagacta gttttgatga
     5821 tgtatttaaa gtaattaaag atcatcctga tttagataaa aaagtattta aagctacgaa
     5881 ggaagttatt caaaatatcg ctacaaacag caaacttcgt tataacacgg caaatgctag
     5941 aacaaaagat gggaagcgac caggtgcaaa ccgctttgat gaaattcacg aaaatgaaga
     6001 ttattcaatg ataaatgtgg ctacttctgg tggtggtaaa attcgagatt atagagaatt
     6061 ttatgataca actaatggtc atgttcgtgg tggtccgctt gatgacatta tagaagaatc
     6121 aaaaatgatt ctttctggag aacttggaat tgacaaggat ggagcagaat tttctagttt
     6181 gtttccattt atttgtcgct tggataacga taatgaagtt gatgatcccg acatgtggga
     6241 aaaagcttgt ccaactatta attacaatgc aaatttaaaa cggaaaatgt ttcaagaata
     6301 ctctcaaatg caacgtaatg ctggtttaag acttacgttc atgaccaaac gaatgaacag
     6361 acctatggaa gatacacgat ttgctgttgc ttcatatgat gatgttctgc atacgaaaga
     6421 aaaagaattt cctgaaaaaa tggatgaagt gataggaaca gtcgattttg ctgatagacg
     6481 agattttgcc agcgttgggt tgctaggaaa atacgataaa gatgtttatt ttacacaaca
     6541 tacttttatc cacgaatcag cccttcgatt acaaaacatc aaacgagagg ttatagatat
     6601 ttctatagat caaggaaaat cacagatcgt tcatggaaaa aatatagaag ctgattatat
     6661 tgtaggttgg tttcttgaaa tgagtaataa atattatatt aaaaaaatcg ctatggatat
     6721 gtaccgtgca aaaatattga agcccgcttt agaagaagca ggttttactg tggaaattgt
     6781 tcgaagcgga tctgttacac atggtatgtt aaaagatctg gttgatgacc tttttattaa
     6841 tcaacgttta ttttttggtg acgatgcgat tatgcgttgg tattgcatga atgtatatga
     6901 agagcatatt tctaatggaa atatacgcta tgaaaaaata gaacctgaaa ctagaaaaac
     6961 ggatggcttt ttttcattcc ttcatggttt gaatttttta gatgatattt atgattctgc
     7021 tcctgtaaca gtcacaaata gctcagtaga aaatacagga actggattta ctcctctagt
     7081 attctaactt gaaaggaggt gagaaagtgg ggatttttca aaaggcggta ggatacttca
     7141 caaaaaaagc aacggttcct ttagaagaat acttttgtaa attgcaagtt gattttgtgt
     7201 atcgaaaatt tgcgattgaa acttgtattg atttgattgc aaatgcgatg agtaaagcgg
     7261 aattcaagtc atatgaagat ggaaaaaata aaaagaatga tctttactat aggctgaatg
     7321 tagctcctaa taagaaaaat aatgcaacag aatttagaaa aaaactgatc aggagattaa
     7381 tattctacaa tgaagtattg atcgtttctc cgtctaataa ttctagcgaa atatttattg
     7441 cggatagttg ggatgtcaca gaatatgcat tgaaagatga tgtgttttct caagtgcaaa
     7501 ttaacaacat agtccttgat agagaatttc tagaaagtga tgttatctat ataaaatacg
     7561 cagatcaaca aattaggcaa ctagtcgatg cgtattatca agcgtatggg aaactcattt
     7621 ctagtgccat gaatgtttac aagcgctcta acgctcgtag atacgtactg aaagggaatt
     7681 tattccgacc gcaagacaat acaacacaag atcaaatcaa taaaatgatg acatcacaat
     7741 ttaaggcttt tatggaagct gataatgcag gtgcggtatt tcaattacaa aatgagtaca
     7801 cattagaaga tttcagcgga aacttccaaa gcaattcaag agatataaaa aacttaatag
     7861 acgacatctt tgagatgaca gcagcagcgt ttcacgttcc gaaaaaccta ctaaagggag
     7921 acatgagtgg gttatcggat caagtggacg cttttttaat gttcgaaatc ataccgattg
     7981 ctgaacttat tcaggatgcg tttaacgcta gtctctatga agtagaagaa tacttgtcag
     8041 ggaattttgt acgtgtggat acaactatga tcaagattac tagcttcaaa gatttggttg
     8101 acgctattga tgtaggcatt agaaatggag tatttacaat caacgaagga agagaacgcg
     8161 ttggaaatga tcgctctgat aaggcgatgg cagatgaaat atttataact aaaaacaatc
     8221 aacaagtatc gaaaggaggt gaggcgaatg acgacaatga aaacatttct agcagtaaag
     8281 aatgaaggcg cagtaccgca aatttttatt cagggattta ttggttctag ttggttcttt
     8341 gaagggaata ctgacaaggg aatcaaaaat attttggata gtctaggtga tcaagaagaa
     8401 attgaagtag taattaattc aaacggtgga gacgtatttc aagggattgc tattgggaac
     8461 ttacttaagt caaataaagc aaaagttaac gttgtgatta acggcttagc cgctagtgct
     8521 gcttcaatta tcgcaatggc tggcgatact ataaaaatct acaacaatgc acaattgatg
     8581 attcaccgcg cttccacata cggagaagga aatgtcgatg acttccgtac gattgctgac
     8641 caactggaat caattgataa atcggtaaag gcttcatata aaacacgatt caatggcaca
     8701 gatgaagcat tgcaagaact tcttgaaaaa gaatcgttta tggatgcaga aacagctttg
     8761 agttatggat tggtcgatga aattatcgat gcagaaaata gctcaggtac tgaagctaaa
     8821 aaagaacaaa gcgttgaaga aattttgaat gacgttaaag aaaaaagagc agaaaaaatt
     8881 gctgcattta cagcagcatt aaataaaaca tttggacaag gagatgtaaa ataatgacag
     8941 ttaaaaattt aaaaggtgta acagctgcaa gcgaccaatt gatgaaagct tttaaagatg
     9001 gtaacgaaga atcttttagc gcagctatgg taagcttatc taaggaaatt caggataaaa
     9061 ttttagaaga agcaacagca aaaaatcaag atcaattagt attaatgaac cgtggtcagc
     9121 gtgtattaac tacacaagaa acaaaattct ataacgaagt agtgaaaaac gaaggttttg
     9181 caggggtcga agaattagtg ccagctactg tatttgaacg tgtatttgaa gatttagaac
     9241 aatctcatcc actattgcaa aaaattactt ttgttaacac aactggtgta acagaatgga
     9301 ttgtgtcacg tggagtcaat ccagcatggt ggggtaaact ttgcgaagct gttaaaaaag
     9361 ttttagataa tggctttgat gtaattaaca tgaagcagtt caagctatca ggttatattc
     9421 ctgtatgtaa ggcaatgctt gatttaggtc cagtatggtt agatcgttat gtccgtactg
     9481 ttttagtaga atcattgaga attgcattag aacaagcaat tgttgatggt actggtaaag
     9541 atatgccagt cggaatgatg cgtgacatga gcaaacaaac tagcggagaa tatgctgaaa
     9601 aaaaagcaga acctattaca gctttagatg ctgtaactat gggccgtttg atggcgcgac
     9661 tatcaaaatt caatatcgaa ggtgtgaatg atccgattta tcgtaatgtg aatccttctg
     9721 atgtggtcct aattgtgaat ccaactgatt actggtctaa agtattccca gctaagactg
     9781 tactaactgc taatggagaa tacgtacaag tattgccagt accagtttca gatttgcagt
     9841 caacggctgt gccagaagga aaagcagtta ttggggtagc ctcagattac ttcatgggtg
     9901 taggatctac actaaaaatt gaagcttcag atgaatacca ttttgttgaa gacgaacgca
     9961 tttatctagc taaacaatat gcaaacggtc aacctaaacg taacgatagt ttcattgtat
    10021 tagatattag cgctttggga actactacta caactacaaa accaacaacc acaacaacta
    10081 caacacaagc gtaggtgatc agaatgaagt atattctttg tcagccggca atcaatcggt
    10141 ttaaatggga gcttgaagtt tgtttaacta atctgaagaa actaggaatc aaagatatcg
    10201 tattgctttt cagcagacac gatgatcaga ttcctatttt ttttgagaag gaatatggtg
    10261 ttgaagttca tgtgtacgat gatctgcggg acgacaaaga gtatattcct tcgattaaac
    10321 catatttatg gtggaaatat ttagaagaag atcattcgcg tgaggacgac cgatatttct
    10381 atatcgattc ggatgtcatt ttcaataaaa gaattaattt gcgcaaattg ccttctaaag
    10441 atgatgtttg gtattgtagc gactgctgta gttatctaag tcttgattat attagaagct
    10501 gtgaaaacgg agaaaatatt ctaaaagata tggcaaacat tgtaaatgtt acagtagaat
    10561 ctttggaaac tataaacact aattcaggag gcgcacagtg ggttattaac cgtcctaaag
    10621 cgaattattg gaaaaaggtt tatctggatt ctaatcggct atatcgctac cttagagggc
    10681 aaaaaacaaa tatacaaatc tggacagccg agatgtgggc acggctttgg aacatgatgt
    10741 atttcaatat tggtcctaaa gttcacgagg aattagactt ttgttttgct actgatccaa
    10801 tagaaaaagt taaagaagta aaaatcttac acaatgctgg agtaacaaca aacgatgaag
    10861 atttattttt caaagggaga tacgtgactt ctacgccttt tgatgaagat ttatcatttg
    10921 taaacaagaa aaaatgctct tacgcatatg ttaaagcaat taaggcggtg gttagatgac
    10981 gcctgaacaa gtgactgaag aattgctaat agctgtgaag gataatattt acgttacctg
    11041 gaacgaagaa gatgagtcaa ttaaaaagat gatagctaaa aatgctgttt atcttcaaag
    11101 taaagtgagt acaacacttt ctttttctcc tgaaagctta gaatacggat tgctaatcga
    11161 aagatgtaga tacgactgga atcgtgcttt agatgagttt gaacaaaatt tcgctagtga
    11221 gttattaggt ttcattcaac attatgcgct acaagaatat attgcaggtg atgtgaatgg
    11281 cgaataatcg tagactcgaa gaaacattca acgatggttg gttaaagatt ttgacgcaaa
    11341 ctaccaaaag aaatgaacta ggaaaaaaga ttggtgtaga agatacagaa atcacttctt
    11401 taaaatttag aaatctttcc atgagagata gtgatataac agctatggat gcgatgggat
    11461 cgaaattaac taagaaagta aagactccat ttcatccaat cgccaagaaa tttaataaag
    11521 atcaatattt tatcgtaatc gatagtatgc gttacaacgt tatctatgcc gattacgata
    11581 atttttatat ctatttttat cttgaaagtg tgggtgaata tggtgattga taattctaaa
    11641 gaaaaagaac gtttaaataa gcaaatttct gctatcaaaa cttccttaga agagcatttt
    11701 ggcctcaaac tctttcaaga ctccgtaggc gaggatgagc tacctgatga ttttaattac
    11761 ttcattctcg aaacaggaga aatagaaatg atcactgagc caaaatatag cgtgggtcaa
    11821 aatctatatc taactttcta ttcagaaaat agagaagatt taacaggaga ctcactagat
    11881 attatttcat tgattcaaaa tcgttcgatt cgttttcaga gaatggatcc caatcattta
    11941 aaactagaga accaagatcg ctatatcgat caattggtat ttacgtttag acgattattg
    12001 aagagtgatt gtcatggcta aaaatagttg ggagctaaaa ataaatggac atgatgaact
    12061 tcttgtgcgg atggaacgct attcaagcga gagcgaacga ctgattaacg aagcattgaa
    12121 atcaaaaggt tcggctattg cagtggatag gattacagaa aaaattcctg tttctgaagc
    12181 agatttaaga agagggcacc aacacgcaaa aaatagtcgt ccacttaaga ctcaatacat
    12241 taatttgggt ttcatcatta gacctacaag aaaatttgag tatttaaaat atcctgattt
    12301 ggggataggt acttctaaaa gaaatcagcc ggacgaattt atgagaagag gattaggtct
    12361 tgcacttgat ccaattacag aacttctgat tcgtcaattc gataaattaa ataaataggg
    12421 ggaacaacaa tggctaaaac aaaaactgta gtaacaacgt tcgataacgt aagtatcaaa
    12481 cgaattgctt ttaattttaa gaacgcagga aatgcaatcg caacgaattg taatggacaa
    12541 ttagatggcg aaacagaaat gcaaacggtg gttaaaaaat gtggagcgac agaagtaaaa
    12601 tcaaaatcta aaccaatcaa tatgacggta acaattactg cacatgtacc gatggaagtt
    12661 tatcgacgtt tcaatgggtt gaaacaagat ggacgtatta aaccaggcat ttactcttac
    12721 ggtcctgatt ccgtaggcga agatttctca cttgctgcag agatcgtgga tgacttcgaa
    12781 gaaaatagaa agttagttgg tatgttagca tgcacttcga atacaggatt aacattctct
    12841 attgaaaatg gtgcggatga agtagctgcg ttagaactag aaacaaaagt tatgcaagat
    12901 gaatttggta aattttatca tgaagcaatt gttgcagaac ttgaagaaga cttaacagat
    12961 caatggatga cgaatctatc tgctgatgtg attaaaaaaa cttcagttgt gacaactacg
    13021 gccactcaat cacagtaaaa aaaacggagg tagcgaaatg aacgaagatt actcaaaaat
    13081 tgaactaaac gatggaacaa ttttgaattt agaacctaaa ctgaatatca agaaattatt
    13141 gatgatcaat agagatttta acacagacga gtttgcaaaa atgactgtgg gaaaaggatc
    13201 catggatatt tctgttattc aaggtgcaaa ggctgtgtat attgcttacc gccaagcgaa
    13261 catgactgat tatatttcat tcgatgaatt tatcgataaa tgggattttg atatggctac
    13321 tgccagctat atttatcaat tgatgatgtt caaacaagca cgcgatgctt atcaaaaaga
    13381 atttgaaaaa gcaaataagg aaaaaaagct tcaaaagtaa aaatgccaaa gctcttagtt
    13441 gaaacgtggg tcgatgtcta ttcgatgttg accgacgttt tttctatgcc ttcagatttg
    13501 gttttaagcg atatctgttt agatgacatt ttgcaaatgg cttacaacaa gagtgcttat
    13561 gaaggatgga aaaactatgc aataaaccaa tctcagaaaa actaaagaaa ggaggtaaaa
    13621 aatggctaaa aagagaacag aagcagaagt aactttcata gctaacgatg acggattgaa
    13681 atctacgtta aaagaaatca gtgctgaatt aactaaaaat agagcagaat taaaactaga
    13741 acaagctcaa ttacaacaga ctggttctga atcagacaag ttaggaagta aattatcttc
    13801 tttagaaaag cagtatgaat tacaaagcca aaaagttgaa gtaactagcc aacgtttagc
    13861 caatgccaaa aaatattatg gagaaaattc caccgaagtt cagaaacttg agagagaact
    13921 gattaaccaa caaacagcgc aacaacgttt gtcaaacgaa attgataaaa cgagtaatac
    13981 actagctcaa gcaaaaggcg aaatacagac gtacgagtct acaatgcaac agttggatag
    14041 tgaacaaaaa aatgttcaag ctagtgcttc tctgattgaa tccgaataca aaaaatggca
    14101 agtaactgct ggtcaatcag cttctgaatc cgagaaatta gcgaaagccc aagaatatgt
    14161 ttctcaacaa tctgaaaatg cagagaaaac gatagatatc ctgagacgac agttagaagc
    14221 tacacagtct gagtttggcg ctacatccac agaagcaatg cagatggagg cgaagcttaa
    14281 tgatgctgaa cgtgaatttg aagagttagg acaagctgct aaaaatgtag atacaactaa
    14341 cttggacgat atcggaagca aaatagatat gaataattta atggaagctt ctgacgtttt
    14401 aagcgacatt ggcgataagc ttacagaatt agggaaacaa gcagtggact ctgctaacag
    14461 tgtaggtagt tcccagagta aaatacaagc taattttggt ttgactaaac aagaggctga
    14521 agaattaacg aatgtagcca gagacattta ttataaaggt tttggagaat cgttagatca
    14581 gtccacagat gcattgattt tggtaaagcg taatttaggc gatttaaata atcaagattt
    14641 acaaaatatt acggaacaag ctatggtcct agaaaacacc atgggcgctg atatggatga
    14701 aacgttacgt ggtgtaaatg gcttaatggt caacttcgga ttgagcgctc aagatgcaat
    14761 ggatttaatg gtttcgggta ctcaaaacgg tttagataaa acgcacgaat taggcgacaa
    14821 tatggcagaa tatagccaat tatggagtca aatgggatat tcagctgatg aaacgttcgg
    14881 aatgcttcaa aatggtttag atgcgggtgc ttataacctt gataaagtca atgacttagt
    14941 taaggaaatg ggaatatcgt taacagatgg tcgatttgag caaaacatgg atatgtttag
    15001 tgaaagtact agaaaagctt ttgaagagtg gaaaaatggt ggaggaacac aaaaagacgt
    15061 tattaattcc atgattcaag attttagcaa tatggatggt caatacgacc aattaaataa
    15121 agcttctaca atttggtctg cacttggcga agataacgcg atgaaagttg tccaatcttt
    15181 gactgatgtt aaccatacat ttgatgatgt tagtggatct gcacaaaaaa tgaatgaaga
    15241 ttctactact ccgttacaag aattaaacgg aaaaatagct gaattaaagg attcattagc
    15301 tcctataggc aacacaatca tagatgcact cgaaccagta attgattttc taggaaagat
    15361 ggctgatgcg tttaataatc ttccacaacc agtacaggat tatgccgtag cgattggcgg
    15421 attgactgct gcatttactt tattaatgcc aataatagtt ggcttcatgg ctctaggtgg
    15481 tcctactaca ttaataatag gagcagttat tactgctatt gctggagtta tagcaattat
    15541 aaaaaactgg ggcgcaatta ctgactggtt taagggaata tggagtaaat tcactgattg
    15601 gttgggtggt acttgggaaa gcataaaaga aggtgcctca tcagtttggg atggagtcaa
    15661 agaaacctgg tctggatttg tagattgggt tcaagaaatt tggcaaggag tttctgattg
    15721 gtttggagag ttatggagcg gattagttga aggagcttcc aacatctggc aaggagtcca
    15781 agagacttgg caagcattcg ttgattgggt ttcaaatatt tggaacggag tcaaagaagt
    15841 atggtcgatt atttgggcag acattgtagg aattgttcaa ataccatgga cattaataac
    15901 gtcattgatt caagctggta ttaatattat cgtgggtatc tttgatgtag ctggacagtt
    15961 attaggcgca gcttggcaag ctgtttggac acctatttct gatttcctta aaaatacttg
    16021 ggatactatg acacaatgga taagcatcgc ttggaatgga attgtaacta cattccatac
    16081 tatatttgat ccagtagtgg catggtggaa tggtatatgg acatctatta gtactacggc
    16141 ttcaaatatt tggaactcaa ttagtgcaac agcttctagt atttggaata gtatcaagaa
    16201 tacaatcact agcttggtac aagcagctgc tacagtaatt caaaatattt ggtcaactgt
    16261 atctagttgg ttaggtggaa tttggaattc aatcagctct acagcatcaa atatctggag
    16321 tagcgtgact agtagtataa gcaatgctat aaacgcagct aaaagtgcca ttcaaagtgt
    16381 ttggaatagt atatcttcgt ggatcagcgg aatttggaac ggtatcaaaa acactgcttt
    16441 gaatctttgg aatggaatta caagcactat tagctctaaa gtaaacgatg gaaaaaatgc
    16501 aatttcaagc ggttggtcca atctaacagg tattgtttcc gacatattca ataatgttaa
    16561 aagcacgata gctaatatat gggaaggtat caaaaagact gttagcgctc cgattgattg
    16621 gattagagat aaaatcagta gtgtctttga taatttgaat atttctatac cacatattcc
    16681 gttaccacat tttaaattga gcggagaatt caatccattg aaggggaaaa tcccaacatt
    16741 gggtgttgat tggtatgcga aaggtagtgt gtttaattct ccaaatatta tcggtgtagg
    16801 tgaatcagga cctgaagcag ttttaccttt gaaaagatct gtgctgcaag aaattggtga
    16861 tcgtatcttg agtagcacct cagtttcatc taggacacaa gcggttcaac ctgtgaacaa
    16921 ttacgaattc aatttcacaa ttgatggtaa cgcagatgaa gtcactatga agcaaacaac
    16981 tcaacaaatc attgatagca ttacaaaagt tcaaaatgat aatgcttcgg catggcgtta
    17041 aacaggagag tatttctcct gtttttttag tattaaaaag gatgtgaaaa aatgactgat
    17101 tgtatacatt ctataatcga tggatttcct gattatttgc ataaattggc tttagctgaa
    17161 agaccaacca taccttctcc aaaaagacag agagttgaaa cttctgtttt aggaaggtta
    17221 ggtggcttag tgcaagatta ctcgtttgaa gacatgtcgt ttacattgca ctataactat
    17281 ttagaggatg tggaagacca tcaagcgttc aagcaatcgt tttatatcat gcgtcattgg
    17341 ttaaactatg caaagaaatt agaattctct gacgatccca acgtctatta cgttatccag
    17401 actatcgata ttggggatgc agaaaacgat attgttgaat ggggagagtt cgatgtaaat
    17461 attactgcga aaccattcgc aagagttcaa gaagatgtac ctataaccgt agataaacca
    17521 cagtcattta acttgctgaa taatagttta gaagaaagtt ttccaaagat tatcatcact
    17581 ccttcagcta cttcatgcca gttcatctta aatgattatg tgtttagttt cgaaggctta
    17641 gtagcaggaa ctgatgtagt cattgatagt gatttgatgc tttgctacga agagcaatcg
    17701 gacggagata ttttagatcg gtccaacaaa atgaagacca tgcaatatcc gacattgcaa
    17761 gtggatatca atcattttaa ttgcactggt ttgagcaaaa tacaaattta tcgtaacggg
    17821 ttaaggtagg tgaaatagat gatcgataat ttaataacta tttacgataa aaatgacgcg
    17881 aataatttag ctgaacattt atatgatacg caaggtttag gcgctttgtc agactggtta
    17941 acagctactg ttagcaataa actaaacgga gccgagatat ttcagggtac ttatccaata
    18001 agcggaacta atgcagattt gattgtagaa ggacgtatta ttcagtgtta tgtagatgaa
    18061 aatcgagcaa aacagcgtct acggatttat tatgcaaaaa cttctgtaat aggcaatacg
    18121 atagaagtaa aagctgaacc tattttcaat gatataagaa aatcggtgtt gaataaatat
    18181 gacagcggaa cagaaaaaat cactgctact caggcatggc aaaacgcaaa agctttagcg
    18241 aaaccagcta tcccttcgca gttttctttc tcgtcattag tagatacgct tgctaatgtg
    18301 aagatagaaa aagcgaattt tttagaattc tttggtggaa aagagggatc tattctagat
    18361 cgatttcatg gggaatttct taaagataat aacacattac atcatgaaaa aaggctaggt
    18421 acggatcata aaatcaaagc gatttatact aaaaatttaa ctggtcttga cttagagata
    18481 gatgctcaaa gcgttttagt tggagtttat ccattcatta gcagttcttc agaaggagaa
    18541 gacgagatca ctctaccaga agaagtcatt ttcacggatt acgtggatga ttatcctgct
    18601 ggatatgttt cttttgttga ttttaaagac aaagcgactg atgtagccac attaagggaa
    18661 gctgctaaag actggttgaa aacaaacata gataaacaaa aaccacaagt gagtggttcg
    18721 attgaattag taccattgag gcatcaaaga ggctatgaaa aatttgttga tctagaaaaa
    18781 gtttcgatgg gtgacggagt agatgtgtat catccacagt taaaagtgaa tatgtcagcg
    18841 agaattgtgg aatatacgtt taatgttcta accaattcat acgataaatt agttgtagga
    18901 aacgtcaaaa caaacttctt agaaaacaca gagaataatg taagcaattt gattaatgat
    18961 gccattgatc aattgaaaaa tggtggcgaa atcagtgatt taatcaatga tattgtagat
    19021 catcaaactg atatgattac tggtcaaaat ggtggttatg ttttattaga tcctaaagaa
    19081 gcgcctagtc gtattttgat tatggacaca ccagataaga ataccgcaag gaatgtttta
    19141 caaatcaaca atgctggtat tggtttttct aaaactggca ttaatggaac atatgaaacc
    19201 gcatggacat tagatggcgg attcaatgcc tcgtttatta cggctggtga aatagtagga
    19261 attactatta gaggtactac attaattagt gatggcactg attatagaac aagtattgct
    19321 aatggcaaaa tgacttggta ctcaaaaaaa gttaacaaag atattatgga gctagaagca
    19381 cgtgattatg taagtgctga tgccggtatt gtatcataca ccatgaaaac tggtggtggt
    19441 ttcatgatta gaaatccaca gggtaacttg gtttttagta cgtgggataa tggtaataac
    19501 agaccgtttc tatcttttgg tgcgcccaat ttcaggtata gcaatgctag ttatgtaact
    19561 tctggcgacg gtagttcttt aagcattaat ggtagtgcgg gtaactcatg ggaatttaag
    19621 gtagctggta ggactatgaa atttactagt gatggtatgc taacgttgcc aggttgtttt
    19681 tttggttcat gggaagatgg gaaacttgct aggtttgaac aatcaacggt acaagtatat
    19741 aaagatttta ctgttagagg tactaaaaac tcaaccgtac caacagaaca ttatggacaa
    19801 cgactattaa acgcttatga aactccagaa tattatttcg ctgattatgg ggaagccgtt
    19861 acaggtgatg atggtaaagt tcgtgttgat attgacccca tgtttgctga gacagtaaat
    19921 ctaagtcgat atatgacaca tgtgacacct acagaactag ttttgtgtgc tgttactcat
    19981 gaagatattg accatttcat cattgaaact agtaagccaa acgtattagt tagatggaat
    20041 ttagtggcac accgtctagg gtatgaagat attagattaa aagaggatac agcatatgat
    20101 agcacagtgc ttgaccaaaa acgtttttaa aacgaagaca aggaggtata taaatggcta
    20161 gcagtttata taatttggct ctagatttca gcaaagaatt aaactacacc aaagctatta
    20221 tggctcgtca aggtgataaa gggattacag tgactgttaa accattttta aatggcttgc
    20281 agatggatac gagtggcgga acatttactt taaaaggaac aacgccatct aaccgttacg
    20341 tagatagtgt ggcaactagt gtaactagtg aagaagtcac gttttctctt gatggcacat
    20401 ttatgagtga agcaggatat tataaacact gctacgtaga atatagaaaa gacaatcaaa
    20461 ttttaacaac gcaagatatc atttttttct cactaggagt gtctgacatt tcgcaaggcc
    20521 aagccgatga atacgtttcg caattggaag agttgattca aaagtataac gaaacttttg
    20581 atgcttttat ggctgaaatc gaaggtagag tggacagctt aaataaacag attactgatt
    20641 taactggtca agctaaaaca ctacaagaca agttagacgc tctgaaagaa gaaatttcta
    20701 aattaggtaa cttgcaggtg atgtacagta acagcatcga
//
LOCUS       DACDXE010000054        20484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.054, whole genome shotgun sequence.
ACCESSION   DACDXE010000054 DACDXE010000000
VERSION     DACDXE010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.054"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            3..551
                     /gene="hslV"
                     /locus_tag="IWM23_001952"
     CDS             3..551
                     /gene="hslV"
                     /locus_tag="IWM23_001952"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAP9884567.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            565..1965
                     /gene="hslU"
                     /locus_tag="IWM23_001953"
     CDS             565..1965
                     /gene="hslU"
                     /locus_tag="IWM23_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAP9884568.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1983..2774
                     /gene="codY"
                     /locus_tag="IWM23_001954"
     CDS             1983..2774
                     /gene="codY"
                     /locus_tag="IWM23_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAP9884569.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2856..3731
                     /locus_tag="IWM23_001955"
     CDS             2856..3731
                     /locus_tag="IWM23_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAP9884570.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWM23_001956"
     CDS             complement(3863..4501)
                     /gene="plsY"
                     /locus_tag="IWM23_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAP9884571.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4628..5062
                     /locus_tag="IWM23_001957"
     CDS             4628..5062
                     /locus_tag="IWM23_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAP9884572.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5228..7273
                     /gene="parE"
                     /locus_tag="IWM23_001958"
     CDS             5228..7273
                     /gene="parE"
                     /locus_tag="IWM23_001958"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAP9884573.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7285..9735
                     /gene="parC"
                     /locus_tag="IWM23_001959"
     CDS             7285..9735
                     /gene="parC"
                     /locus_tag="IWM23_001959"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAP9884574.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10012..12243
                     /gene="pflB"
                     /locus_tag="IWM23_001960"
     CDS             10012..12243
                     /gene="pflB"
                     /locus_tag="IWM23_001960"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAP9884575.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12487..13248
                     /gene="pflA"
                     /locus_tag="IWM23_001961"
     CDS             12487..13248
                     /gene="pflA"
                     /locus_tag="IWM23_001961"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAP9884576.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13333..14259
                     /locus_tag="IWM23_001962"
     CDS             13333..14259
                     /locus_tag="IWM23_001962"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAP9884577.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14441..15334
                     /locus_tag="IWM23_001963"
     CDS             14441..15334
                     /locus_tag="IWM23_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAP9884578.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15468..15593
                     /locus_tag="IWM23_001964"
     CDS             15468..15593
                     /locus_tag="IWM23_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9884579.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15727..16359)
                     /locus_tag="IWM23_001965"
     CDS             complement(15727..16359)
                     /locus_tag="IWM23_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884580.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16507..17049)
                     /locus_tag="IWM23_001966"
     CDS             complement(16507..17049)
                     /locus_tag="IWM23_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAP9884581.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17184..17573
                     /locus_tag="IWM23_001967"
     CDS             17184..17573
                     /locus_tag="IWM23_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884582.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17577..18341
                     /locus_tag="IWM23_001968"
     CDS             17577..18341
                     /locus_tag="IWM23_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9884583.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18470..19417
                     /locus_tag="IWM23_001969"
     CDS             18470..19417
                     /locus_tag="IWM23_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884584.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19547..20287
                     /locus_tag="IWM23_001970"
     CDS             19547..20287
                     /locus_tag="IWM23_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884585.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7192 a         3481 c         4316 g         5495 t
ORIGIN      
        1 acatggtcga atcaccattt cattcaacca ctatctgtgc cgtcgaaaaa gacgggaaat
       61 ttgctatggc tggagatggc caagtaacaa tgggtgaatc tgttgtcatg aaaggcactg
      121 caaagaaagt acgacgtatc tacaatggag aagttgttgt aggtttcgcc ggaagtgttg
      181 cagatgcgtt tactttagaa gaaaaatttg aaggcaaact aaacgaatat aacgggaatc
      241 tacaacgcgc agctgtcgaa ttagctcaag aatggcggac acagcaatct atgcaaaaac
      301 ttgaagcgat gctgatcgta atgaatgata aagaaatgct tcttgtttca ggtacaggag
      361 aagttatcac accagatgat ggtatcttag ctatcggatc aggagggaat tttgcgttgg
      421 ctgcagcccg agcaatgaaa aactatgctc aaaacgacat gagtgcgaaa gaaattgcag
      481 aaaatgcgtt aaatattgcc gcagatatct gtatctttac taatcataat attatcgtag
      541 aagaactgta agaggtgagt agatatgtat gaattaaaac aaacaccaag acaaatcgta
      601 agtgaattag atgaatatat cgtaggacag cacgcagcaa aaaaatctgt tgccgttgct
      661 ttgcgtaacc gctatcgccg tttgcagtta gatgaaaaaa tgcaacaaga tattacacca
      721 aaaaatatgc tgatgatcgg acctacaggg gtcggtaaaa cagaaatcgc acgacgtttg
      781 gcaaaaatcg tcaatgcacc atttgtcaaa gtcgaagcaa caaaatttac tgaagtcggt
      841 tatgtcggaa gagatgtaga atcaatggtt cgggatttag tagaaaatgc gatccaaatc
      901 gtagaaaaag agcagtacag ccgtgtatac acaagtgcta aaaaaaatgc agaaaaacgt
      961 ttggtaaaaa tcttggtacc tggtatcaaa aaagaacaga aacaatccac taatcaattt
     1021 gaacaaatga tgaatctttt taatgctagt cagcaaaata ccgctgaacc gcaagaagaa
     1081 gtgacagaag atattcgaat caatcgccgt acagtcttgg aacaactaca aaagggttta
     1141 ctggatagtc gtgaagtaac cattgaagtt gaagaccaaa agaaacctgc accaatgatg
     1201 aacaatggat tggagcaaat ggggatcgac ctaaatgaaa ctctaggtgc tttgacaccg
     1261 aagaaaaaag tggaacggac ggtaacagtc aaagaagctt tagagttatt gatcaaggaa
     1321 gaatcagcaa aattagtcaa ggatgctgat atccatagtg aggcaatcaa attagcggaa
     1381 gaaagtggaa ttatttttat cgatgaaatc gataagatca cttctaaatc acagcaatct
     1441 ggagaagtat ccagagaagg cgtccaaaga gacatattgc caattgtcga aggttcacaa
     1501 gtaaacacaa aatacgggat catccaaaca gatcacgtcc tattcattgc ttcaggagct
     1561 ttccatttgt ctaaaccaag tgatctaatt ccagaacttc aaggacgctt cccgatacgg
     1621 gtagaattag atgatttgac agctgaagat tttgtgaaga ttctgaccga accaaataat
     1681 gctttagtaa aacaatacgt cgcgttgatc ggtacagaaa atgtgtcagt tacgtttaca
     1741 aaagaagcaa ttgaacggat cgctcaaatc gctttcgatg tcaaccgtga cacagataat
     1801 atcggcgcac gcagactgca taccattttg gaaaaactac tagaagatct attatttgaa
     1861 gcaccagata tgcaaatggg agaaataacg atcacagaag catatgtgga tgacaaactt
     1921 ggctcaattg cggcaaatga agatttaagt cgctatattt tataaaaaat cggaggacga
     1981 caatgacaga cttgctaacg aaaacccgca agataaacac acttttgcag caaaagaata
     2041 cttttgacat gcaagcagat ttgccttacg ataaaatggc tgtcacacta ggtggtattc
     2101 ttgacagcaa cacctatatc ataggaagtg acggagtatt actaggttat aatgaacgtc
     2161 acgatgtgaa caatgaacga atcaaaacaa tgtttgttga gaaacaattt cctgaatctt
     2221 atactgatac agtggcacag ttgatgaaaa ctgaagctaa tatcccaatt actagtgata
     2281 ttactgcatt tccggtagag tcaagagaga aatatccatt tggtctgacg actgtcgttc
     2341 cgatctttgg agctggcgaa cgtttgggta cgattatctt atctcgtatg gaacaatcgt
     2401 ttgcagatga cgatctagtc ttagcagaat atggagctac ggttgtcggt atgcagatcc
     2461 tttatcagaa gtctcgaagc atcgaagcag atgtacggag tgcaacggct gtccaaatgg
     2521 caatcaacac attatcttat agcgaattga aggctgtaca agctatcttt gaagcattgg
     2581 atggagatga aggtcgtctg actgcttcta atattgccga tagtatcggc atcactcgtt
     2641 ctgtgatcgt caatgcttta cgaaaattgg aatcagctgg aatcattgaa tctcgttcgt
     2701 taggcatgaa gggaacttat ttaaaagtat tgaattcccg ttttaaagac gagttagcaa
     2761 aacacagtta ttagaagtaa ggggactggg gcagatagaa atgtttgttc cagccttccc
     2821 attttaaaaa gtaagtcgaa gttggaggaa tggcaatgac tgttattatt caaaatgacc
     2881 agctgatcgc cgaaatcgct gaacacggtg cggaattgat cagtctgaaa tcaaaagaac
     2941 atggtttaga gtatatctgg caaggtgatc cagcgtattg gggcagacat gcaccggttt
     3001 tattcccttt tgttggacgt ttaaaagatg atcagtatgt ttatcaaggg aaaacatatc
     3061 aaatggggca acacggattt gctcgggata tggattttga agtcattgaa catggacaag
     3121 aaatggctag tttcatctta aggagtacgc cggagacatt agaaaagtat ccatttgatt
     3181 ttgaattagt gatcagctat gaattaggag gagacggcat cacggttcat tatcaagtgg
     3241 caaataccgg tgaaaaagaa atgttctttt ctatcggtgg acatcctgct ttcaatgtac
     3301 ctcttgaaga aggattgtca tttgaagatt tctatctggc attttctcca ttgaaatcaa
     3361 gattacgcct accattgaaa ggcccattca tcgatatgga tcaaaagacg ttaggtcaga
     3421 caaatacgaa tctggctttg aatcgtgaac tttttgagca agatgccttg gtttttgaaa
     3481 caaggggtct aaatgcgtac gccattcgaa gtgaaaaatc ttcccatagc gtaactttaa
     3541 gctataaaga tatgccttat gtagggatct ggtctcctta tccaaaagaa gcgcctttcg
     3601 tttgtatcga accgtggtgc ggaatcgctg acacggtgaa cagtagtgga aacttgatgg
     3661 aaaaagtagg gatcaatcaa ctaggtgccc atgaaatctt caagacaaaa tattccatca
     3721 cagtgaagta agaggttgtg accgagaagt tagcagggaa caccgtttac caaggtgtgt
     3781 aaaaaagcta tcctgtaagt aataagggag tgtgacatcg atttgtcaca ctcccgtttt
     3841 atctctttgg aatgaaaaag tatcaggatt ttttcttgct gctattcaaa ccaaacggga
     3901 ttcgactttc agtatggttt ctgatacgct tgatattgtc tttatgccga taaaagataa
     3961 aggaagaaac taaaaaggca atgatcgtca gtaaccaatt gggcttagcc aaaatcacag
     4021 gagctacata aggaaggacg atcgtagata aagtgatcaa tactgcactg atcatactgc
     4081 tcaaactcac catacttgta caaaataaac taacgataaa gatgatacaa gaataaacaa
     4141 aaaatacagg attaaatcct aaaagcattc ctgcacttgt cgcaacagct tttcctcctt
     4201 tgaacccagc aaaaatcgga aatgtatggc caagaatggc gcatacccca aaccaaagag
     4261 ggttaatccc agaaaggtgg aagatcatcg gcaaacaagt agccaatgta ccttttaaaa
     4321 tatccataag caggacaaca atcccggctg ttttgcctaa tacacgaaat gtattggtcg
     4381 ttccagtatt accgctcccg tgttgtcgga tatctttttt gaaaaacaat ttgccaatcc
     4441 agacaccgga aggaatagaa cctaaaagat atgctaaaat aagtaataag atcaatttca
     4501 ttttttaccc tctttcaagt tgaaaactat cctttatttt agcatgaata ggtaacagga
     4561 acaattgaaa aaatgaaaga tacaagtata atagatggaa taaaaagaaa agcaaggggt
     4621 ggcagaaatg tctgtaaaaa atccaacaca agaaacgatt ttcaactatt tggaagaagc
     4681 aaaaaatatt gccgtcgtag gcttgagcaa taaaccagaa cgaacaagtt acatggttgc
     4741 acaggtattg caagacaatg gatataaaat cataccagta aatccattat tggctggtga
     4801 agaaattcta ggcgagaaag tctatgcgaa tttgacagaa atcgatgatc caatcgatat
     4861 cgtagatgta tttagacgga gtgaattttt acctgaaata gcaggtgatt ttttgaaaac
     4921 tgatgctaaa gtgttctggg cacaattagg gattgaaaac aaagaagcag ctgagatgct
     4981 aagaaaagca ggacgtacag atatcgtaat ggatcgttgt attaagattg aacttggtaa
     5041 gttgaaagaa aaccaaaaat aaaaatgacc acaaagaaga gatgatcgat agaggggaat
     5101 gtcgaaaaag cggttaggag ataattaaaa ttgatttctc gtctaccctc ttttttttca
     5161 cgaccttttc tagtatgata atcgtggtgc ggttttgccg caaagtatta ttgaaggagc
     5221 gtttgccttg gcaaaaaaag taaacaacga atacaatgac tcgtccatcc aagtattgga
     5281 aggattagag gcagttagaa aaagaccagg gatgtacatt ggatccactg acagccgagg
     5341 cttgcatcat ttagtctatg aaatcgtcga taatgcagtt gacgaagcat tatcaggtta
     5401 tggcagtgag attgatgtga ccatccatga agacaatagt ataactgttg cagatagcgg
     5461 acgtgggatg cctgtaggaa tgcatgcttc cggaatacca acagttgaag tgatttttac
     5521 tgtgctgcat gctggcggaa agtttggtca aggagggtac aaaacttctg gtggactgca
     5581 cggtgtagga gccagtgtgg taaatgcctt atcgaaatgg ctaattgtaa cgatcgttag
     5641 agatggcgta gagtatcagc agaaatttaa aaacggcggt aaacctgacg gtactttgaa
     5701 aaagatagga aaaacaaaaa aagcaaacgg gacgactgtc cattttttac cagatgatac
     5761 aatattcagt acaacgaaat tttcatatga aattttagcc gaacggttaa gagaatctgc
     5821 ctttcttcta aaaggcgtta aaatctctct ttctgatctt cgtggagaag aaccagtcaa
     5881 ggaaattttt cattacgaag aaggaatcaa agaatttgtt gattatctga atgaagaaaa
     5941 agatacattg acaccagttg tttatttttc aggagaaaaa gaaggtatcg aagtagaggt
     6001 cgcttatcaa tacaatgacg gttattcaga aaatgtttta tcttttgtca ataacgttcg
     6061 gacaaaagat gggggaaccc atgaagctgg tatgaaagcg gcgatgacga aatcttataa
     6121 tgaatatgcg agaaaagttg gtttgctgaa agaacgggac aagaatctgg aaggtagtga
     6181 tttcagagaa gggttagctg ctgtactttc tatccgtgta cctgaaaatc ttctgcaatt
     6241 tgaagggcaa acaaaagaaa aacttggtac gccagttgct cgtaccgtag tagataacgt
     6301 catcagtgaa caaatgggct tttatttgca ggaaaatagc gaaatgagcc agatgttggt
     6361 ccgtaaagca atcaaagcca gagaagcgag agaagcagca cgtaaagcca gagaagaaag
     6421 ccgaaatggg aagaagcgta aaaaaggcga atctcttctt tcaggaaaat tgacacccgc
     6481 acaatcccga aaccctaaga aaaatgaatt gtatttagtc gaaggggatt ctgctggcgg
     6541 atctgctaaa caaggaagag acagaaagtt ccaagcaatt ctacctttac gaggaaaagt
     6601 catcaatacc gaaaaagcta aaatgcaaga tatcttgaaa aatgaagaaa tcaatacgat
     6661 gatctatacg attggtgcag gagtcggtcc tgagttttct atcgaagact gtaattacga
     6721 caaagtaatc atcatgaccg atgcggatac agacggcgcc catattcaag tattactgct
     6781 tactttcttt tatcgttata tgaaaccgct gattgaagct gggaaagtat atatcgcgtt
     6841 accgccttta tataaagtat ccaaaggaca aggaaagaag caagtcattg aatatgcgtg
     6901 gacagatgac gaattggcag caatgattaa aaaagtcggc aaaggatata tgctgcagcg
     6961 atataaagga cttggagaga tgaatgcgga acaattgtgg gaaacaacga tggatccgac
     7021 ttcccgtacg ttgatccgcg tccgtatcga tgatgctgcg caagctgaac gtcgagtaac
     7081 cacactgatg ggtgacaaag tagaacctcg aagaaaatgg atcgaaaatc acgtgcaatt
     7141 tacgctcgaa gaagatggca gtatcttaga taaaaaagag gatactgaaa tctctccttc
     7201 agtttctaat gatttattag atgaagaacg agcagataaa aatgaaaaca atcagttatt
     7261 tgaagtagaa taggaggtca cctgatggaa aatcgacaag aagtacaaga attaacttta
     7321 gaagaagtga tgggtgaccg cttcggacgt tattccaaat atattatcca agaacgtgca
     7381 ctgcctgata tccgtgatgg attgaagcca gtgcaacgcc ggatattatt tgccatgaac
     7441 aaagatggca atacgtatga caaaggattc cgtaaatcag caaaatctgt cggaaatatc
     7501 atggggaatt atcatcctca tggtgacatc agtatttatg aagcgatggt ccgcatgagc
     7561 caagactgga agttgcggga agtactgatc gaaatgcacg ggaataacgg gagtatggac
     7621 ggtgatccac ctgctgcgat gcgttatact gaagccagat tatccaagtt aagtggagag
     7681 atgctagcag atatcgagaa agaaacagtc gatctcgtgt ggaattttga tgatacagaa
     7741 aaagaaccaa cagtccttcc agctcgatac ccaaaccttc tcgtcaatgg atcgacaggt
     7801 atatctgctg gatatgcaac agaaatcccg actcataact tagctgaagt gatcgatggc
     7861 acaatctata tgatcgatca tccacaagcg agccttgaaa aattgatgga atatattcct
     7921 ggacctgact ttccaacagg agggatcttg caaggaaaag atgaaatcaa aaaagcttat
     7981 gaaacaggac gaggaaaagt tattcttcgt tcgaaaacaa agatagaggc aatgaagggt
     8041 aataaacagc aaatcgtgat cacagaaatc ccatatgaag taaataaagc gaccttggtg
     8101 aaaaaaatgg atgagatccg tttgaataag aaaatcgatg gaatagctga agtacgagat
     8161 gaaagtgata gaacaggtct gcaaattgtc gtcgaattga aaaaagacgc caatgctcaa
     8221 ggaattttga attatttgtt caaaaacact gagctacaga tcaattacaa tttcaatatg
     8281 gtcgctatcg accatatgac tccgcatcaa gttggactga aagacatttt aagaagttat
     8341 atcgagcatc gaaagcaagt cataacaaaa cgtagtcaat tcgatttagc caaagctcaa
     8401 aaaagacagc atatcgtaga aggattgatg aaagctttat cgattctgga tgaagtaatc
     8461 gcaacgattc gtgaaagtaa agacaaaaaa gacgccaaaa agaatttagt cgacgtcttt
     8521 caatttacag aagaacaagc agaagcaatt gtcacattgc aattataccg tttgacgaac
     8581 acggatatca cagagcttca aaaggaatcc gaaagcttga ttgcgcaaat cacagagttg
     8641 aataaaatcc tttcaaatga caaagagctt ttctctgtta tgaaaaaaga attgcgagaa
     8701 gtgaaaaaaa attattcttc cgctcgtcta acgacaatcg aagacgaaat cgaagaaatc
     8761 aaaattgata cgcaagtctt agtggcacaa gaagacgtaa ttgtcagtgt gacgcgagaa
     8821 ggctatgtga agcgaacaag ccttcgttct tatagtgctt ccaaaccaga agaaatcggg
     8881 atgcgagaag gcgactactt gctatactca ggtgaattga gtacattgga ccatgttctt
     8941 ctgatcacaa ataaagcaaa tgtgatctac cgccctgtcc atgaactacc cgatttgaaa
     9001 tggaaagatg ctggtgagca catttctcaa acaatctcca atctgtctgt agacgaatcg
     9061 attttagctg ttttcccata tcaaaaaatt caagcagaga aaacatttgt attcatcagc
     9121 aagaatggat tgatcaagca aacacgaatg acagagttcg aaccatggcg cacctataag
     9181 agccgtccgc tcagctgtat gaagctaaag gcggaagaag atgaactgat tgctgtgtat
     9241 ctgaaagaag atcagccagg cttagatgtt ttcttagtaa cgcatcaagg aatgggtttg
     9301 cgctatcctt taacagatgt tcccgttgtt ggagcaaagg cagctggagt gaagtctatg
     9361 aatttgaaag atggagatag gattatcaac gggctgcttg tgttagcaga aggtgatacg
     9421 ccgatcgtaa tcgttactca gcgaggagct gttaaacgca tgcttgctca agaaatcagc
     9481 caaacttcac gtgcgaagcg gggagtgacg gtcatgcgtg agctgaaaaa aaatccgcac
     9541 cgtattatct atatgtcgga aggaagatca aaagaaataa ccttgatgaa tcaaaaaggg
     9601 cagcagttaa cgattgaccc aactgatttc cctataggtg atcgaacatc gaatggatca
     9661 tttgcgatgg atgagaaaaa aggtggagaa gtcatcgaag ttatcgatgc tcctgagatt
     9721 ttcatcgagt cgtaactgtt gtagtatttt aatgaagaga cgccatctgt attaatacag
     9781 gtggcgtctc tttttcattc atttttaata aatccttata cgtttgcaaa aaaagatgaa
     9841 agtgttcaca atgcttaaat gttggtatgt caacgttgat ttaaatatat gtaaataaaa
     9901 cataaaaatt gttaaataaa aaacaagaaa acccttgcaa agatagggtg ctgtgttata
     9961 ctaatcacgt aaaaactgtt acataaaaca gaaatttagg aggtccatac aatggagcaa
    10021 tggaatggat ttaaaggtac caaatggaaa gaaggcgtag acactagaga ctttattcaa
    10081 aataactaca ctgaatatcg cggtgatgac agctttttgg aaggcccaac agaagcaaca
    10141 gaaaaattat gggatgaatt gcaaaagtta aatgatatcc aattcgagcg taacggtgtc
    10201 tatgatatgg acacagaaat cgtatcaact attacttcac acgatgcagg ctatttggat
    10261 aaagatttgg aaaaaatcgt cggtctacag acagacaaac ctttgaaaca agcattcatg
    10321 ccgttcggcg gaatcaatat ggctaacaat gcgctaaaaa gcaatggcta tacaccaaac
    10381 gatgaattga cacatatttt cactgactgg cgcaaaacac ataatcaagg tgtatttgac
    10441 gcttatactg ctgaaatgcg tgctgctcgt aaaaacaaaa tcattacagg gcttccagac
    10501 gcttacggtc gcggacgtat catcggtgac tatcgtcgtg tggcattgta tggaatcgac
    10561 tatttgatgg aacaaaagaa aaaagatcat gatatgactg gttatcatac aatgagtgaa
    10621 gaagtgattc gtttacgtga agaaatcact gaacaatatc gtgcattagc gcaaatgaaa
    10681 gaaatggctg cgaaatatgg ctatgatatt tctaaaccag ctgcaaatgc aaaagaagct
    10741 gtccaatggc tatacttcgg atatttagca gcaatcaaat cacaaaatgg tgctgcgatg
    10801 tcaatcggac gtatctcagc attccttgat atctatatcc aacgtgattt ggataatgga
    10861 gtaatcacag aaaaagaagc acaagaattg atcgaccact tagtaatgaa attacgtatg
    10921 gtcaaattcg cgcgtacacc tgaatataac caattattct caggattccc tatctgggct
    10981 actttatcca ttgcaggtat gggattagat ggtcgttcat tagttacaaa gaatgatttc
    11041 cgtctgcttc atacattaac aaatatggga ccttcaccag aaccaaactt gactgttttg
    11101 tattctgaac accttccaga aggattcaga acatatgcta gcagaatcgc tatcgaaagt
    11161 tcttcgattc aattcgaaaa tgataatcag cttcgtgaac aatggggttc tgatgattgt
    11221 gcgattgctt gctgtgtatc aggaacagtt gtcggaaaag acatgcaatt ctttggcgct
    11281 cgtgctaact tggctaaaac actattatat gccatcaatg gcggagtcga tgaagtgact
    11341 ggcgcccaag ttggaccaga atatcgtcca atcacatcag aaatactaga ctttgaagaa
    11401 gtgaaacata acttcatgaa tatgatggat tggttagcag aactatatgt caatacgttg
    11461 aacatcattc attatatgca tgataaatat tcttatgaag cagctcagct ggctttgatg
    11521 gattctgact tgcaacgcac atttgctaca ggtattgctg ggatctctca tgcggcagac
    11581 agcttgtcag cgatcaaata tgcaaaagta aaagcaatcc gtaatgaaaa aggaatagca
    11641 gttgattttg aagtggatgg cgacttccct aaatacggaa atgatgatga ccgtgcagat
    11701 gaattagttt catggatctt ggaatacttc atgggacaaa tcaaacgtca taaaacttac
    11761 cgcaactcaa aaccaacaac ttcattattg acgattactt caaatgttgt ttacggtaaa
    11821 aatacaggaa atactcctga tggccgtgaa gctggaaaac cacttgcgcc aggagcaaat
    11881 ccaagttaca atgcggaaca aagtgggtta ttagctagcc tgaactcaac agctaaaatg
    11941 aattacggct gtgcattgga tggaatttca aatacccaaa caatcaatcc ttcaggtctt
    12001 ggaaaagaac aagatacaag aatcgaaaac ttgcgtaatg tcttagatgg atacttctca
    12061 aaaggagctt atcatttgaa tgtcaatgta ttcagtaatg atttattgcg tgatgcaatg
    12121 gaaaatccaa tgaaatatcc gaacttgacg atccgggtat ctggttatgc agttaaattc
    12181 cgtgacttga caagagaaca acaattagat gtcttgatga gaacttcaca cgacagaatg
    12241 taaaaagttc gtgaaaaaag tttttcgact tagcgtcgaa aggacgttga atagccatcg
    12301 catcaaaatc gaataatcag tatgcttatt tgaaagaaac gatttacagg ggcaggactt
    12361 gtatgttccg tccctgtaaa ttttacacat aacgaagcgg caattgcgaa aaattttgtt
    12421 cagaaagctt cgcttcgaca gctaacttgt acttaaaatg aaatcaaaga aaagatgtga
    12481 acttaaatgg aagaaaaaac aatcggctat gttcattcta tcgaaacttt cggttcagta
    12541 gacggacctg gactgcgctt tgtcgtgttt atgcaaggat gccgcatgcg ttgccaattt
    12601 tgtcacaatc cagatacttg gaatattggc ggagggaaag aatacacagc agatgaactt
    12661 ttagataaag cagaacgctt cagaccttac tggggagata aaggtggtat tactgttagt
    12721 ggtggagaac ctctattgca gatcgatttt ttgatcgaac tatttaaaaa agcaaaagag
    12781 cggaagatgc atactacatt ggatacatgc ggaaaacctt ttacgtatga agaacctttc
    12841 ttttcacgtt ttcaagaatt gatgaagtat acagatttac tgttatttga catcaaacac
    12901 atcgataatg aagagcataa aaaactaacg cattgggaca atgaaaatat tttagaaatg
    12961 gctcaatatt tgtcaaaaat caacaaaccg gtttggattc gccatgtact agttcctgag
    13021 cgaagtgatt atgatgaata tttgatccga ctagacaact ttattcaaac cttgtcaaat
    13081 gtagacagag tagagattct tccttatcat acgatgggaa aatacaagtg ggaaacactt
    13141 ggtttgaaat atccattaga agggatcgaa ccacctacaa aagaacgtgt cgaaaatgct
    13201 aaacgattgc ttcatacaga agagtataca aaatatttaa cgcgataggt cgcatcggaa
    13261 agaagataag tattgcttct ttccgttttt ttgctattat taaataggag aaatcacaaa
    13321 ggagcgaatg acatgtcaaa aattttagtt tttggtcacc aaaatccaga tacagatgca
    13381 atcggggcag ccattgcgtt ttcacacttg caaaatgaat taggtaaaga aacagaagca
    13441 gttgctctag gtacacctaa tgaagaaaca caatacgcat tagaccactt tcagctatct
    13501 gcaccacgag tagtagagtc agctaaagca gaaaatgcag atgtcatgct tgtggatcat
    13561 aatgaattcc aacaaagtat ttcagatatc agcgaagtaa atattttagc tgtagtagac
    13621 caccatcgta ttgcaaactt tgaaacagct aatccattgt actatcgtgc agaaccagtt
    13681 ggatgtacaa gtacgattat tttaaaacta tataaagaaa accaagtaac aattcctaaa
    13741 gaaatcgcag gaatcatgct ctcagctatc atttcagata cattgttgtt caaatcacca
    13801 acatgtacgc ctgaagatga agcagctgca aaagaacttg cagcaatcgc tgaagtcgat
    13861 ctaaacactt atggacttga aatgttgaaa gcaggaacaa atctaagtga taaatcagct
    13921 gcggttcttc ttgatttgga tgcaaaaagt ttcccaatgg gcagttccaa cgttcgtatc
    13981 gctcaagtca atacagtgga ccttaatgaa gtgatggatc gacaagcaga gctagaagca
    14041 gcaatggaag atgaaatgaa agcaaatggc tacgatctgt tcgtattagt tgtcacaaac
    14101 atcttagaca gtgattcaga attacttgtt gctggtaaag gaaaagaaaa agtagaagaa
    14161 gctttccaaa caactttgac caacaaccgt gctttgctta aaggtgtcgt ttcacgtaaa
    14221 aaacaagtcg tacctcaatt gacggctgta tttagctaat caaatggtag tatgaagcat
    14281 tcaaaatgaa tggataagcg attgatataa aaagaaacat agaacatact aaaggccgaa
    14341 gaaatggagg agaataacag tcaattatga ctcttgttct tcctcctttt tacgtaaaag
    14401 ccttttaagt aaaataaaac ggatagaaaa gagaacatat atgaactatt attcaaaaaa
    14461 taaggaaaac ttttatcaaa aactgaccga tgatccgctt ttttcttcat tgaccgatta
    14521 tctatatgaa catcgagaac aagaaacgat tctaagagaa ttgaaaaaag aattttcgca
    14581 aaataaattt tcccattttc ttgatttatt gatcgatgca ggattaatca agagggaaga
    14641 acgaagatat catttgaact ttccaatatt tgatcctaaa gattacttgc aacaagccac
    14701 ttcggctgct gaaaccatag ctgaacaatt gaagagactc tctgttgatg aacaaaaatt
    14761 agcgatggga gaagttattt gggcgtattg tttcgaagat gaacggaaag aagcgtattt
    14821 ttatggagta agaaacagcc gagagacaga attgctgagg acaactgcag gaaatgagaa
    14881 gtaccgtttt atcacacttt cctctataga acacttcccg cttactttgg ctaattactt
    14941 ttttgtacag aagaatcagc ttcctgtaac gaaggcattc aaagaattag ccgaacttat
    15001 tggagacgta aatgaatcgt acttttttga tcaaatagaa gttatcgttg acaggatacg
    15061 taagaataaa tataaaaatc gccggcctag tattttccat cagtctctac ttgtgacgaa
    15121 tactataaag gaagaagaat cattcacatt agaattacct attgtcgaaa aaaacaattt
    15181 tgagattgaa ttaccaacat tggatccttc attgacaatg gaggaaatgg cctttcttaa
    15241 acgacaaatc ttttctgagc tgagcaagaa atttataccg catgcatttt cttatatcaa
    15301 agaatatgga acagttttag tgagtaaaac ctaagagagc agtaagcaaa aattatactg
    15361 actcttgcag gaggagcctt tctcttcctt tatggttaaa agaattattt gagtattttg
    15421 cctatctacc acctattaga ttcactacat ggtctggaaa caaaggaatg caaaaaaatg
    15481 gtgagaaatg tggaatgaca aaggaagtag tgataagaaa agttagattt ttaaataatc
    15541 aatattatga ttcagtcaag tatggcattt tatgggaaga actagctgat tagattagga
    15601 agtccatttg ataatccaga atcactaaga aacgaatggc gagtcattaa taatttatcc
    15661 aatcttgggt tattctttta gattgaagtg tttaaaacta aaaaagcatt tatgaagtcg
    15721 taatagtcag gcttcaaaat gctttttatt ttgtttgttt tttctttgcc acataaatac
    15781 acctagactg acgatgaaga gcatatccaa cagatttcct gcgacttgga tcgtcgaagg
    15841 gttggtcagt ggcattaaag caaggacaaa aaaggaaaac aagctgtaaa taaaacaggt
    15901 gacaggaact aatcgaaaga agagcggaca tttcaattgg ctaaaaatga gtgaaaaaat
    15961 aaaataagta aaaatggata aaatcacgac taaaccattc atgataaaga agaaaagcgt
    16021 attgtgatta aacgaataaa aaagaattgc taaaaaagaa aacgttttaa atgcaatcaa
    16081 tacgcttgaa gcttcgttta acgtaccagg atgtcgatga aaaaaatagg caccgaaata
    16141 aacagaaaac agtaagccgc aaagagaaga caaaatcaaa aaaaacggat tttcttcggc
    16201 ttctgtgata tcagaatgat ccaaaagcaa ccagcctaat gcactagaag gcgcatgtga
    16261 ggtcttaagg tatgaataac atagaaaaaa taagatcgtc aaagttgcaa aaaaagagcc
    16321 taaatgtaaa aaattagtgg ttctacgttg aatttccata atatctccaa attaaattga
    16381 taagtatata aagtataata acaaattgat gagtatatgt cgatgttttt taatcaaaat
    16441 aataaataaa aactgtctaa catttaaaat ttaatttaga tattagacag tttttaagtt
    16501 tgtgagttat tttccttgtc gttttctcac ttttttagtc cagaaaccac ctgaaatata
    16561 ttttggctca taagaaatga taaaagcacg ctcttcgatt tctttgatca ggcgatacag
    16621 ttcacgttcg ctttttctag gagaaagtat ttcgagaact agccgctcac cttccagccc
    16681 cattgcagaa gtctgtgtca caccataacc gtgttctctc agtatacccg gcaaactttt
    16741 atcttctgcg ctatttgctg gcaaaatcgt tgtgaccatg atgtatccta atgccaattt
    16801 gtcttcaatc aaaattccga cactgatacc tactgcgtaa cctaacgcat agacaaataa
    16861 attcagtgga ttatctaaac gatctaaaac aagggataag ccgacgatat aaatcgtgat
    16921 ctcgaccata ctgacaagtg gagcaatcag tcgatagcct ttcattgtca acatgaaacg
    16981 taacgtgttc agagtgatat aagcaaaatt aatgaggaag atcatcccca acatttttgg
    17041 atcaatcatt tgtaaaactc ctttcattta tataacaaca atatcaaaaa aaatcgtaag
    17101 tgaagaaaaa ttgaaacttt atacatacaa aaaaatgata aaatgattat aatagaatta
    17161 taaagatagg ttaggtgatt aaaatgaaaa cggtcaatcg tgtgttttgt gccagccacg
    17221 tgctaagtct ccttctcagt atatacttag gtgtctattc gattcaacag ccaattgagg
    17281 tagagaacat tgtttttcag atgtcgctga cagatggttt aattttaagc gtttttttct
    17341 ttttgatact gtttattggt aatattttcg gtagcgttgc cagttttttg aatttttcta
    17401 tttatccact cctttcgcta gcattaggag tggtgggact ttgttctctg tgcttatttc
    17461 ccgagccatt cagtcctgca tttatattat ttggtacact gaacctgttc cagacaactg
    17521 ttggagcatg gttgctatgg cgatcaggaa atttaatgaa aataggtgag taacaattga
    17581 atccaattat attgaaacgt tctgtccatc atattccagt gttagaagtt gttctgaaag
    17641 aattaagaga ggaacgtatt cccgttgtca tctattatca cggatggcag acaagtaagg
    17701 agttagtttt gacgcaagga agaaaaattg cgcagcaggg cattcgtgtg atcctgccag
    17761 atgcgatgaa tcatggggag cgcaaacagc cggtttcttc tattccatcc tatacatttt
    17821 gggatagtat ctacggaaat ctgtttgaat ttgacacact tatcaagcat ttagaaaaaa
    17881 gaggtcttct acatgatcag cttgcagttg gcggggtttc gatgggggga atgacaacat
    17941 gcgccttgtt ggctaaacat cctcatatag tagcgggcgt gtgtttgatg ggttcccctg
    18001 cacctttgga atatggaaaa gaaatcgtaa ggcgcgcatc ggaatataat tatgtcgtac
    18061 caaaagacta ttttgatttg atttcatggg ttaagagcta tgatttgtca ttgcagccgg
    18121 aaaagttagc tggacgtcct ctgttctttt ggcatggaca agctgacgaa aaaatccctt
    18181 atatacaaac ggaacgattt gtagaaaaaa atcggcataa gtcttacgga aaagaaattg
    18241 tttttcgttc ctcaaaaaca cagcgacata tggtacaaat accactaatg gaagaagctt
    18301 ccactttttt agcaaaaaat ttgataaaaa agtataaata attgaaacgg aaaatcactc
    18361 atgacgatag aggattttcc gttttttatt ttgggctata ataaactctg ttaggaaaat
    18421 ttggtataat tgatagtaag aatgacaaca tgttagaaag gaagagagca tgcccacttt
    18481 agcagatgtt gcaaaacgtg ccaatgtttc aaaaatgacg gtctctcgag tcatcaatca
    18541 tccggaacag gtgactgacg aattgaaaga gttagttttc tcagcaatgg ctgaactgga
    18601 ttaccgtcca aatattgctg caaaagcact tgtcacgaat cggtcgcaga tcatcaaatt
    18661 atttatactg gaagaaatcg acacaactga gccctattac atgaatttgc tgatgggaat
    18721 cgctaaacgg atcggtaaag cgcattattc tctccaatta gttacaaatg atgccttcga
    18781 cgtcggttct tgtgatggat acatcatcac aggtgtcaga aaaaaagact tcgattggat
    18841 cgaccgtttg gaaaaaccag ttgtcttgtt tggggaaaat tctgaaggat atgattatgt
    18901 agacagcgat aatgaatatg gtacagcaaa agcaacgcaa tatgccttag aaagagatta
    18961 tcaaacgatt atttatatcg gtatcgaagt ggacgagcct tttgaaattt caagggaaac
    19021 tggttattta tctgtgatga aggaagttga tcttcctagt aaaatcttgc ggtttccaaa
    19081 tcattcgaca gaagtagaaa gatatatgac agaaaactgg gcagatttta cagataaaac
    19141 ttgttttgta tgtagttcag atcggctagc actaggagca gtaagaggga ttataaatgc
    19201 tggaggacac ttgcccgaag aatttggtgt gattggattt gatggggtat ttttagacca
    19261 aatcagttca cctaaattga caacgatcaa gcaagatatt atacgtatgg gtgaagtatg
    19321 tggagaaatg ttgttaaaaa aaatagaaga aaaaggggcg agtcaaggtt atcgtcgttt
    19381 ttttcctgaa ctagttatca gagagacaac gaattaagtc ttattactat atatatattt
    19441 atgaaattca tcgctatttt cttaaaataa tcagcgcatt gctactgaaa acaatgaatc
    19501 tgtgctatta ttgagaaaag ttccattttt gagaaggatg gtttgaatga aagaaatcag
    19561 acgaaaacat ttccgttttc ctgcgtacga tgatgagtta ggcgtaaaat taaccaacga
    19621 caagaagcga gtattatttg acggacaaga agatcttttg actgaacaga atcagtcccc
    19681 gtttgaacag atgaaagaat ttcatttcac gaacacgaac accgagaaaa tccgtcgcaa
    19741 gcgtcgtgca acaaatggat tgaagtcagc tccgcgtaag gaaaatttgg aaaagcataa
    19801 aggaaaatta ccggattata ccggccatta ccaatcagaa atcacatcga caggacgaaa
    19861 aaaacttttt ggtgattcct ctcgttctgt cagacaaagt gaaacaagga cagagtccaa
    19921 gcatactaca cgaaacgcat ctgttgacac gaaggtgcgg gagcgttctt attttgttcc
    19981 taaatatgta cccgcatcga ttatcccaga ccctagagaa agcaaaattt ctgaggatca
    20041 tttaatcgaa tcgatgaaaa aaacggatga ctcttatctg ttgttcgata cagaaccgac
    20101 gccgtatcaa gaaaaaagaa atggtgagcc gtctgtacga acattcaatc gaacaaaaac
    20161 gttagaggaa acaaagcagt cagaagaaaa aaaatcgaaa aaaagccatg gtatcttaga
    20221 acgctcatta caaggattga tcgatgatca aggaaactct ttgggagaaa atagctattt
    20281 taaataagtg atatacaaat ttatattcgg ctacagttgg aaaggaaacg ctgcggcgtt
    20341 tccttatttt atactctatc cgaaagttta tatcgtatcc tcaaattttt ttgagtttaa
    20401 gcgaattttt tctcccaata agtaagaaaa taatggcaaa ataaaaaaac aaactatttt
    20461 cgcagatcgt ttgcaaaaat agtt
//
LOCUS       DACDXE010000055        20216 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.055, whole genome shotgun sequence.
ACCESSION   DACDXE010000055 DACDXE010000000
VERSION     DACDXE010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20216
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.055"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..504
                     /locus_tag="IWM23_001971"
     CDS             <1..504
                     /locus_tag="IWM23_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884586.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLKNNE
                     VILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDKVR
                     YMFNKIT"
     gene            623..790
                     /locus_tag="IWM23_001972"
     CDS             623..790
                     /locus_tag="IWM23_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9884587.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(850..1203)
                     /locus_tag="IWM23_001973"
     CDS             complement(850..1203)
                     /locus_tag="IWM23_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884588.1"
                     /translation="MRKKEDKYDFRAFGLAIKEARLKRGLTREQVGALIEIDPRYLTN
                     IENKGQHPSIQVLYDLVSLLHVSVDEFFLPANNLVKSTRRLQIEKYMDSFTDKELSLM
                     ESLASGINEARNIED"
     gene            1708..2130
                     /locus_tag="IWM23_001974"
     CDS             1708..2130
                     /locus_tag="IWM23_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9884589.1"
                     /translation="MKPSSFQTTIENQFDYICKRAMEDERKNYMLYLSRIAKREVSFS
                     DVGDYLVSQFATTDNYSTDFQIFTLNGLSVGVENDLLSEALRELPDKKREILLLFYFM
                     DMSDSEIADLLKLNRSTVYRHRTSGLALIKKFMEEFEE"
     gene            2127..2357
                     /locus_tag="IWM23_001975"
     CDS             2127..2357
                     /locus_tag="IWM23_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9884590.1"
                     /translation="MKTQYPMIPFPLIVKATDGDTEAINQILHHYRGYITKRSLRLMK
                     DEYGNQSMVVDEVLRGRMETRLITKILSFEIK"
     gene            2818..3021
                     /locus_tag="IWM23_001976"
     CDS             2818..3021
                     /locus_tag="IWM23_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006730246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9884591.1"
                     /translation="MKQTDIPIWERYTLTIEEASKYFRIGENKLRRLAEENKNANWLI
                     MNGNRIQIKRKQFEKIIDTLDAI"
     gene            3103..4320
                     /locus_tag="IWM23_001977"
     CDS             3103..4320
                     /locus_tag="IWM23_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020220015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAP9884592.1"
                     /translation="MSEKRRDNKGRILKTGESQRKDGRYLYKYIDSFGEPQFVYSWKL
                     VATDRVPAGKRDCISLREKIAELQKDIHDGIDVVGKKMTLCQLYAKQNAQRPKVRKNT
                     ETGRKYLMDILKKDKLGVRSIDSIKPSDAKEWAIRMSENGYAYQTINNYKRSLKASFY
                     IAIQDDCVRKNPFDFQLKAVLDDDTVPKTVLTEEQEEKLLAFAKADKTYSKNYDEILI
                     LLKTGLRISEFGGLTLPDLDFENRLVNIDHQLLRDTEIGYYIETPKTKSGERQVPMVE
                     EAYQAFKRVLANRKNDKRVEIDGYSDFLFLNRKNYPKVASDYNGMMKGLVKKYNKYNE
                     DKLPHITPHSLRHTFCTNYANAGMNPKALQYIMGHANIAMTLNYYAHATFDSAMAEMK
                     RLNKEKQQERLVA"
     gene            4494..5339
                     /locus_tag="IWM23_001978"
                     /pseudo
     CDS             4494..5339
                     /locus_tag="IWM23_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            5913..6482
                     /locus_tag="IWM23_001979"
     CDS             5913..6482
                     /locus_tag="IWM23_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAP9884593.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            6469..7329
                     /locus_tag="IWM23_001980"
     CDS             6469..7329
                     /locus_tag="IWM23_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9884594.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            7519..8223
                     /locus_tag="IWM23_001981"
     CDS             7519..8223
                     /locus_tag="IWM23_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884595.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            8228..10063
                     /gene="walK"
                     /locus_tag="IWM23_001982"
     CDS             8228..10063
                     /gene="walK"
                     /locus_tag="IWM23_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAP9884596.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            10060..11376
                     /locus_tag="IWM23_001983"
     CDS             10060..11376
                     /locus_tag="IWM23_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884597.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            11377..12246
                     /locus_tag="IWM23_001984"
     CDS             11377..12246
                     /locus_tag="IWM23_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884598.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            12301..13110
                     /locus_tag="IWM23_001985"
     CDS             12301..13110
                     /locus_tag="IWM23_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9884599.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(13213..13842)
                     /locus_tag="IWM23_001986"
     CDS             complement(13213..13842)
                     /locus_tag="IWM23_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HAP9884600.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            14012..15304
                     /locus_tag="IWM23_001987"
     CDS             14012..15304
                     /locus_tag="IWM23_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9884601.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(15380..16042)
                     /locus_tag="IWM23_001988"
     CDS             complement(15380..16042)
                     /locus_tag="IWM23_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9884602.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            16267..16551
                     /locus_tag="IWM23_001989"
     CDS             16267..16551
                     /locus_tag="IWM23_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAP9884603.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            16602..18227
                     /gene="groL"
                     /locus_tag="IWM23_001990"
     CDS             16602..18227
                     /gene="groL"
                     /locus_tag="IWM23_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAP9884604.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(18316..19464)
                     /locus_tag="IWM23_001991"
     CDS             complement(18316..19464)
                     /locus_tag="IWM23_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884605.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(19566..20150)
                     /locus_tag="IWM23_001992"
     CDS             complement(19566..20150)
                     /locus_tag="IWM23_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9884606.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
BASE COUNT         6708 a         3422 c         4135 g         5951 t
ORIGIN      
        1 tttcaaaatg cagttatgga ggggatacgc tatggctgtg aacaaggatt gtatggttgg
       61 aatgtgacgg actgtaaaat ctgttttaag tatggcttat actatagccc tgttagtacc
      121 ccagcagatt ttcggatgct tgctcctatt gtattggaac aagtcttaaa aaaagctgga
      181 acagaattgt tagagccata tcttagtttt aaaatttatg cgccacagga atatctttca
      241 cgagcataca acgatgctcc taaatattgt gcgaacatcg tagacactca attgaaaaat
      301 aatgaggtca ttcttagtgg agaaatccct gctcggtgta ttcaagaata tcgtagtgat
      361 ttaactttct ttacaaatgg acgtagtgtt tgtttaacag agttaaaagg gtaccatgtt
      421 actaccggtg aacctgtttg ccagccccgt cgtccaaata gtcggataga taaagtacga
      481 tatatgttca ataaaataac ttagtgtatt ttatgttgtt atataaatat ggtttcttgt
      541 taaataagat gaaatatttt ttaataaaga tttgaattaa agtgtaaagg aggagatagt
      601 tattataaac tacaagtgga tattgtgtcc tgtatgtgga aataaaacac gattaaagat
      661 aagggaagat actgaattaa aaaaattccc cctctattgt ccgaaatgca gacaagaaaa
      721 tttaattgaa ataaagcagt tcaaagtaac tgtgattaca gagccagacg caaagacgca
      781 gagccgataa aatgagatta atacaatctc attttatcgg ctctttccgt tatgtatgga
      841 ttcttttaat tagtcttcga tgtttcttgc ttcgttgata ccgctggcta aagattccat
      901 taaggatagt tctttgtctg taaagctatc catgtatttc tctatctgta atcgtcgggt
      961 gctttttacc aagttattag caggtaagaa aaattcatca acggaaacat gaagtaacga
     1021 tacaaggtca taaagaactt gtatgctggg gtgttgccct ttattttcaa tattagttaa
     1081 gtaccgtggg tcaatttcaa tcaatgctcc cacttgttca cgagttaaac ctcgtttcaa
     1141 tcgagcttct ttaatggcta aaccaaaggc tctaaaatca tatttatctt cttttttacg
     1201 catagtagac cacctctata cattttattg ttcctactga attaaaaaca ggtatagaaa
     1261 aacgtgttat atggtttata ggtttatatt taataaaaag cactactaaa cgccaataaa
     1321 aaaaaccgtt atatggtagt gctatttacg ctgttaaaat attgtatatt acttccaaat
     1381 ggcggtttgt tggaggtcaa cgtcgccatg aagtacatca tatacaataa atttccttac
     1441 attgggttct tgtcaaaaaa agtcgtctat ctgcaataga taagtacgtc caccaatgtg
     1501 gttttataaa tcatatagat agaataacag aagcatgtaa acagagaaat aaatctgttt
     1561 atatgctttt ttggctattc agaacttttt tacaaagttt atttatcagt aatgcaacaa
     1621 atcccccttt cacattggga ctaagagtga aaggagataa acgagcaagg ctcacttcct
     1681 ttcctagaca gaaagggggt gagaaacatg aaaccatctt cttttcagac cacaatagaa
     1741 aatcagtttg actatatctg taaacgtgct atggaagacg agcgaaagaa ttatatgctt
     1801 tatctttcaa ggattgcaaa gcgtgaggtg tccttttcgg atgttggcga ttatcttgtt
     1861 agccagtttg cgacaacaga taactattca actgactttc agatttttac actcaatggg
     1921 ttatcagtag gcgttgaaaa tgatttgttg agtgaagcat tacgtgagtt gccagacaag
     1981 aaacgtgaaa ttctactgct gttttacttt atggacatga gcgattcaga aattgcagac
     2041 ctgttgaaat tgaaccgttc tactgtctat cggcatagaa ccagtggact agccttaatt
     2101 aaaaagttta tggaggaatt tgaagaatga aaacacaata tcctatgatt ccctttcctc
     2161 tcattgtaaa ggcaacagat ggcgataccg aagcgattaa ccagattcta catcattaca
     2221 gagggtacat aacgaagcgt tccctacgac ttatgaaaga tgaatatggc aatcaaagta
     2281 tggtcgttga tgaagtctta cgtggaagaa tggaaaccag actgattaca aagattttgt
     2341 catttgaaat taagtaatat cctctctcct ttcgtggaag cgtgctaaac cattccacgc
     2401 ttcccgaaca gggaggtttg ttattccacc aaagcatatt gagctttcaa tgtgttttga
     2461 taggctaacg agccattgtt ctttgaaaac tgaataaaag taatcgaata cgtttcgata
     2521 agaaaagagc caacggaact aaccgccatg acctatctta taaagatagc gagcgattca
     2581 tgttagtgat ccgagaagca atctttagca ggattgcctg caacgacatt cttatcgtga
     2641 taatgatact cccatacagt caatagtccg agcgtgataa aaccgtcgca ggcaatgagt
     2701 atggctacat gagaaccatg caggggtgga actcccgtga gctttgctaa agctgttcga
     2761 ttgctggtaa aacaactttt atgaaatcca aataagtgat ttggaaagga ggattttatg
     2821 aagcagactg acattcctat ttgggaacgt tataccctaa ccattgaaga agcgtcaaaa
     2881 tattttcgta ttggcgaaaa caagctacga cgcttggcag aggaaaataa aaatgcaaat
     2941 tggctgatta tgaatggcaa tcgtattcag attaaacgaa aacaatttga aaaaattata
     3001 gatacattgg acgcaatcta gcgtcgccaa agggtcttgt atatgataaa atagtattaa
     3061 gtcgtatcaa ggctctttcc ataaaggaaa ggagcaaatg ccatgtcaga aaaaagacgt
     3121 gacaataaag gtcgaatctt aaagactgga gagagccaac gaaaagacgg aagatactta
     3181 tacaaatata tagattcatt tggagaaccg caatttgttt actcgtggaa acttgtggct
     3241 acagaccgag taccagcagg aaagcgtgat tgtatctcac ttagagagaa aatcgcagag
     3301 ttacagaaag acattcatga tggtattgat gttgtaggaa agaaaatgac actctgccag
     3361 ctttacgcaa aacagaacgc tcaaagacca aaggttagaa aaaacactga aactggacgc
     3421 aaatatctta tggatatttt gaagaaagac aagttaggtg taagaagtat tgacagtatt
     3481 aagccatcag acgctaaaga atgggctatt agaatgagtg aaaatggtta tgcttatcaa
     3541 accatcaata actacaaacg ttctttaaag gcttcattct atattgctat acaagatgat
     3601 tgtgttcgga agaatccatt tgactttcaa ctgaaagcag ttcttgatga tgatactgtc
     3661 cctaagaccg tactaacaga agaacaggaa gaaaaactgt tagcctttgc aaaagctgat
     3721 aaaacctaca gcaaaaatta tgatgaaatt ctgatactct taaaaacagg tcttcgtatt
     3781 tcagagtttg gtggtttgac acttccagat ttagattttg agaatcgtct tgtcaatata
     3841 gaccatcagc tattgagaga tactgaaatt gggtactaca ttgaaacacc aaagaccaaa
     3901 agtggcgaac gtcaagttcc tatggttgaa gaagcctatc aagcatttaa gcgagtgtta
     3961 gcgaatcgaa agaatgataa gcgtgttgag attgatggat atagtgattt cctctttctt
     4021 aatagaaaga actatccaaa agtggcaagt gattacaacg gcatgatgaa aggtcttgtt
     4081 aagaaataca ataagtataa cgaggataaa ttgccacaca tcactccaca tagtttgcga
     4141 catacattct gtaccaacta tgcaaatgca ggaatgaatc caaaggcatt acagtacatt
     4201 atgggacatg ctaatatagc catgacgctg aactattacg cacatgcaac attcgattct
     4261 gcaatggcag aaatgaaacg cttgaataaa gagaagcaac aggagcgtct tgttgcttag
     4321 tagtacaaat gaatttacta cttatttacc acttctgaca gctaagacat gaggaaatat
     4381 gcaaagaaac gtgaagtatc ttcctacagt aaaaatactc gaaagcacat agaataaggc
     4441 tttacgagca tttaagaaaa tataaaaaga taattagaaa tttatacttt gtttatccta
     4501 aaaaaattaa actaaatagg ttaaagcctt atcatttaca agagttttat caatatatgt
     4561 taaatgataa ctgtacagca aatacggtta ttcattatca cgcatttata cgcaaagctc
     4621 ttcaagaggc tgttattaca gaattgataa gtacaaatgt agcagataaa gtaagaaagc
     4681 caaaaaagaa acaatttgtt tctcaagttt ataatcaaca agagatcttg aaattacttg
     4741 acattttgtc agaagaaaaa ctctatttgg tagttctttt aacagcattt tatggattgc
     4801 gaagaagtga agttttagga ttaaaatggt ctgctattga ttttttaaac aaaaaaatca
     4861 tgataaacca tgtaatcata gaaaatccag agaatatgtc acagttaata aagaaagatc
     4921 aaacaaagaa ctcttccagc tatcgtacat tacctttggt tgaaacgatt gaacgagcat
     4981 tagtaaatca agctaaatgg caacaagata atcgtattct tttaggagat gattatattt
     5041 tagaagataa tgagtacgta tttactatgg aagatggacg gttgatgaag cctcaatatg
     5101 taacgcatcg tttgagtaag ttgattaaga aaaatggatt gaaaaaaatt cgctttcatg
     5161 acttgcgtca ttctaatgct tcaataatgt tagatagtgg ccagaatatg aaatccattc
     5221 aagaatggct gggacatgca tcgtattcga caactgctaa tttatacacg catttaaccg
     5281 caggagtaaa agatcgtgca gcagaagcat tagaaaacgc atttgggttt aaagaataaa
     5341 aaaacaaaaa acaatatctt tggggtatcg cttcttgttt ttagaatttt tttgttggca
     5401 atttgttggc agtatgttgg caatgaaaaa attcacaaac taaaaaccta tttataaagc
     5461 tttataaaac ttttttcaca aatagagcat ttggttaaga gtcagctgct ctaccaactg
     5521 agctaacggc ccgtacaaaa agtacttaat aatagtagca ctaagaattt taaaatgcaa
     5581 gactacattt aaagaaaatc agaaaaatct ttacttcctt ataggaaatc attcatagat
     5641 tattcacatt tgctcgctat tatataagca taccaacaaa caaagttctt gcaagagaac
     5701 aaaccctaac acttttttca ttttttactc ctttcccgcc gtctccccgg cgggtctcct
     5761 gttcttaacg ataaaagaca agaaggctta gccttcttgt tttttgtttt catggaatcc
     5821 cgctaatcaa atcacctagt taaagcaact agattgaatg tttatcataa gtctgatatc
     5881 atgtttatga aatgaaaaaa ggagcggtta tgatggaaaa aatgcgtcag caattaatgg
     5941 agtgggggaa ggaactagaa acattccgtc tgcctcactg ggaagaactc cctgacttag
     6001 aactttatat ggaccaagtc agaacgttag ttgatcgtta tctttcacct gtgatacaag
     6061 gagaaaagca tccattgctt acctcttcta tggtaaacaa ttacgttaaa ctaggtttga
     6121 taccagcacc agttaaaaaa agatataaca aagaacacgt agcattttta cttgcaatca
     6181 ctacgttgaa acaagtattg actattcctg aaatcaaaga agggatattg ttccaaggaa
     6241 aaacagtagg aattcgtgaa gcctataatc tattttgcga tgaacaagaa gcagctgtgt
     6301 ggatggtcag ccaattagca caaggtaaat ctcatccgca aaaattcgac caggcaaccc
     6361 ctgtagaata tattgctgta agagcggcaa ctttatcgtt tgcaatgaaa cttcttgcgg
     6421 aaaaaacgat tgttctagaa accgaatatt taaaagagga gaaaacaaat gaaaaacaat
     6481 aaaatcgcaa ttttagtaga ttcaggtacg gatgttccag aagaattgat aaaaaaatac
     6541 gagatgtata tgatcccatt gaaaattatt tataaagatc aggtatatac agataaaatt
     6601 gacattaccc ctgaagagat ctatcaacgt ttgccagagg aaatccctgg tacatctttg
     6661 ccggatggtg agtcgatcac agaaatcttt gagcaaatca aaaaagacgg ttatgaaaag
     6721 gtacttgcgg taacaatttc cagtggattg agcgggacat ataatgtggt tcgtttaatt
     6781 gcacaacagc aagaagaatt ggaagcatat gtactagata cgaaaaatat tggtattggt
     6841 gcaggttttt ctgcgatcca agcagcaaaa tggctggaag aaggagtaga atgggatcaa
     6901 ctgatcagta atctgaatga gttagtcgaa agaacaaaag ttttctttaa tgttgcgaca
     6961 ttggaatatc ttcaaaaagg tggacggatc ggtttagttg cttctattgt aggaaccgct
     7021 ttaaagctta acccaatcat ctcatgtaat gaagaaggga tttattacac agtaggaaaa
     7081 gctaggggac gtaaaaaaag tttggataaa acggttgctt acgtcaaaga aaaaatcggt
     7141 aatgcaaagg tcttcaactt agctgttgcc catggggatg cgaaagaaga agcaattgaa
     7201 atggaagcga gacttaaaag agagttccca caagcaaacc aaatctactt tggccagatt
     7261 tctccggcat tagtagtcca cactggtccg ggattattgg gtgtcggtgt acaagtttta
     7321 gaaggataaa tcaaaaaaag gaaacactga ggtgaaaatc agtgttttcc ttttttattg
     7381 aagttagaac aaaaaataat aaagtatttt gcactttcat tttaattttc tcttttgttt
     7441 tataggcaaa gatgaaacaa aagcagaaat acggtataat gagaaggtat gacaataata
     7501 tataagaggt ggctctttat gaaaaaagtt ttagttgtcg atgatgagaa gccgatttcg
     7561 gatatcgtca aattcaattt agccaaagaa ggctatgacg tttatactgc atatgatggg
     7621 gaagaagcct tggaaaaagt tgctgaggta gaacctgatt tgattttact tgatttgatg
     7681 ctacctaaaa tggacggctt agaagttgct cgtgaggtac gcaaaactta tgatatgccg
     7741 atcattatgg taacagccaa agattctgaa attgataaag tcctaggctt agagttaggc
     7801 gccgatgatt acgtgaccaa gccattttcc aaccgagaat tagttgcacg tgtcaaagca
     7861 aatcttcgtc gcggtgccac ggctgcgaag gaaccagaag aggcagcacc tgcggaattg
     7921 acgattggtg atttgacgat ccatccagaa gcttacatgg tgacgaaacg aggagaaaca
     7981 atcgagttaa cacatcgtga attcgaacta ctcttttact tggcgaagca ccttggacaa
     8041 gtgatgacaa gagaacattt attacaaacg gtttggggtt atgattattt tggagatgta
     8101 cgaacagtgg atgtaactgt acgtcgtttg cgtgaaaaga tcgaagataa tccaagccat
     8161 ccaaattatt tagtgacacg tcgtggtgtc ggttactatt tgcgtaaccc agaacaggag
     8221 taattggatg aaaagaaaag tccgcttttt tcgatcagtc aattttaaga tcgctattac
     8281 ctttattctg attttattga tctcgatcga aattattggt gcctacttta tacgtggttt
     8341 agaacgttcc acgatcaata catttattaa agatatgaat caaacggtgg aatcattagc
     8401 aacaacaatc agcccggaat tgaatcgaaa agataatgca gatgatgaag aggtaaatgc
     8461 gaatatcaaa agatttattg aaaacagtgc gactagcgat atcatcgaaa tacgggtcgt
     8521 tgacgaaaaa gggatcatcc gaggaacaac cgatgtcaat gaacaaagcg ctgtgggaaa
     8581 gaaaaatgat tatgttgata tcaatgattt cacgactaaa cgttatgttg ctttagataa
     8641 cgacgataaa cgggtgaata tcaatgttca accgatcctg tccccgaccg gcgatgccgt
     8701 tatcggggct ctttacgtca aaagtaatat cgaacaaaaa tattcggaaa tcaataacac
     8761 agcagtcatt ttctttactg cctcactaat tgcggcattt atttcaatga tcgtatctgt
     8821 tttagttgct cgttcgatta cacagccgat cggtgaaatg agagaacaag ccattcgaat
     8881 cgctagagga gactacagtc ggaaagtcaa agtgcatgga caagatgaac ttggacagct
     8941 ggctgatacc ttcaatcagt tagctgaacg tattgaagaa gctcaagata caatggaagc
     9001 tgaacgcaat cgtttggata gcgtgctatc ccatatgaca gacggcgtca ttgcgacaga
     9061 tcgcaggggt aaagtcatta cgatcaatga tatggctgtt tccttgctag atatcaaaaa
     9121 cgaggaagca atcggacaat cgattttgac acttttagat atcgaagaag aatatacgtt
     9181 gcggaaatta ttagaagatc caaacgaaat ggtacttgac cgttcaacca gtacattgga
     9241 aagtgatcag atgatcttgc gagtagactt ttctatgatc cgtcgcgaat caggttttat
     9301 cagcggatta gtagctgtct tgcatgatgt gacagaacaa gaaaaaacag aacgggaacg
     9361 tcgtgaattc gtctctaatg tttcccatga acttcgtaca ccgttgacaa gtatgcgtag
     9421 ttatatcgaa gcattgagcg aaggtgcgtg gaaagacgag gaagtagcac caaatttttt
     9481 gaaggtaacg ctagatgaaa cagaccgaat gattcgaatg atcaatgacc tcttaaatct
     9541 atctcgcatg gatacaggta atacccagct tcaattagaa tatgtcaatt tcaacgagtt
     9601 agtgaatttt gttcttgacc ggtttgatat gatggtggga aatcaggaaa aaaattataa
     9661 gatccgtcgt gaatttacgc aaagagatct ttgggtcgaa gtagatacag ataaaatcat
     9721 ccaagtagtc gataatatca tgaataacgc gatcaagtat tcaccagatg ggggaacgat
     9781 tacttgtcgc ttattagaaa cgcataataa cgtgatttta agtatcaccg atcaaggatt
     9841 ggggattcct aaaaaagatc tgaaccgagt gttcgagcgg ttctatcgag tagataaagc
     9901 acgtgcaaga gcacaaggtg ggacaggact tggattagct atttctagag aagtgattaa
     9961 ggcgcatcga ggcgctattt gggcagagag caaggaagga aaagggtcga ctttttatat
    10021 ctcgctgcca tatgaacctt atgaggagga atggtgggaa tgagaatctc tgaaaaagtc
    10081 gttcgtatag gactcatttt gatggttgcg ttaagcatat acttctctta tgcaatctgg
    10141 ctgagcccag caggaaaaag ttcaatcaac gtagatgaga caaacactca aatgatcgag
    10201 tctcaaagca atcgaaaaac ttccgaagtc tttcttccgc ttcatgttac atggcaccat
    10261 tcgggaatga tccaagaaac caacagtgaa aatctcgtgt accgattgca attgatcatc
    10321 gataaagcaa gatttggaaa gctttctgaa gtagtttcag gagacaaatc acagtttgaa
    10381 caggtgaaaa atctgaactc tggttttgag ttggcctata atgcgccttt tttgctaaga
    10441 gaatacaagg aagcatttga tttagattta gatgtgtcga acttggcaaa agaaagcgaa
    10501 aatatgtatt ttactagaat acaattcgat caaggaaata aaaaaatccg atttttgaat
    10561 tattcgaacc atctcgtata tgaagcaaat atttcgttga attggacaga tatcaataaa
    10621 gaattaacag caaaagacac aaaatggaca gaaatgacaa cagaaccatc gacgatcgac
    10681 acgcaatatg ctacgaaaca ttcgatcaaa ctgaagaagt atagttatat cctgtcacag
    10741 caaccctata cactttttag aaatgctttt ttccagaaac cagatagcgt gaaaaataat
    10801 gatgaaagca atgaattgat tttttacggt gggaatgaaa caatgtctat ccattcggaa
    10861 acgcaagtag ttgattttcg tggagagctg ccagagaaaa aagaaaatgc tagtgtattt
    10921 tctgaaagtt tcccctatat tagcaaatta ggcagttctt tagggaacct cagatatttt
    10981 gaccgcagtg acaatacgat cgattatcgg atattcgtgg aaggcttccc ggtttttggg
    11041 acagacagca gaggacagat tggtgtggaa gttggcggtg aaacgaatgg ttctgtgcaa
    11101 gtgaatatcc aaaccagttt gaatacgatt caagtgccga taccttcaga cgaagaagta
    11161 gaacttgcaa gtacgaatga aatcatccag cagttaaaag ctaagggagc agatagcaac
    11221 aagattggtt cagtgatcgt tggctataca tggcaaaatg tcaaagaaac gaatcgtgtc
    11281 gttgatctct tacctgaatg gtatgtgaaa tataaagata actggtattc agctaacgac
    11341 cttctggaaa aattacctgg attggaggcg aattaattgg atttcaaaaa aatcgaatgg
    11401 atatttttcg tcgctttttt agggttgaat gtgtttttat tcagcagtta tcatgacgcg
    11461 gtataccaag aaaacaatgt cattcgctct aatcagactg aatcaatcca acaacgtctg
    11521 gctagcgacg atatcactta cacggggaag atttcgtctg agaacaagca aggctattat
    11581 cttagcgcgg agcagaccaa tcttagcact tcattagcga attatcgcaa ggctggtaaa
    11641 agcggcttgc tggcaagtgg tgtgttttta gacggtgatg ttttgacgaa atctttatcc
    11701 agttcagatg ctgaaaagaa cgtgatcgat ccagataaaa ttagtagttc cttggaagat
    11761 tttttatcta acaaagacaa tattttattc ggtaatgatt atgtgtacac gaaaaacttt
    11821 tctcatttag aagaagaacc actagaaatc caagcaagcc aagagtatga aggcatccca
    11881 ttcaatgatg atacagcaaa aatcgttctt tctttagaaa aaaacgataa tgattatagc
    11941 atcacgaaat acatgcagac gcatttatct gatatcgagc agctgcgcga aaagacggaa
    12001 ctgtatacgg aagaggaagc aatcaataca ttgtacatca ataacaaaat accaagagga
    12061 tcaaaaattt tatggcgtca gctagcttat tcctgtattc tgaaagtccg ggaaaaaaat
    12121 gtgtatgttc ctgtttggca tgtagcgatc gaaacaagcg ataaagtggt ccaaattgag
    12181 agcgtcaacg ctttcagcaa tacgatcgtg acaaacaata ctgtaccaaa agtggaagat
    12241 caataaaaaa gcgagtataa tgaaaaaagt ttacaaagta agaagggaag taggagtcaa
    12301 atggctgaag aaaaaccagc ttttaaaatc agtatcctag caagtggaag ttcgggtaat
    12361 tcactttata tcgaaagtga aaaaaaacgg cttttggtcg atgcaggact tagcggtaaa
    12421 aaaatcactt ccttgatggc tcaaattgat cggacaccgg accaattaga tggaatactt
    12481 gtgacgcatg aacatcgaga tcatatacat ggcgtgggtg tcttggctcg taagtatcat
    12541 ttggatgttt ttgccaatca gcagacttgg gaagcgatgg aaccactgat tggaaatgta
    12601 ccagtggaac aaaaacatct atttgagatg gggaaagtca gaacattcgg cgatatcgat
    12661 attgagagtt tcggcgtctc ccatgacgca gcggctccgc aattttatcg tttccataaa
    12721 gaaaatcggt cattcgtcat gttgacggac accggatatt gcagtgatta tatgcgaggt
    12781 gtcattgaaa atgccgatgg ttatttgatg gaaagtaatc atgatcttga gatgttacgc
    12841 atgggacctt atccgtggaa tttaaaacag cgtattttag gagatagagg acatctttcc
    12901 aacgaagacg gtgcacttgt catgactgat gtgatcggta atcggaccaa acgaatctat
    12961 cttggccatc ttagcaagga aaacaatatg aaagaacttg ctcatttaac aatggaaaat
    13021 gttttgaaag aaaaagattt tggcgttgga catgattttg aaatctatga tacagatcca
    13081 gatgaagcaa ctgagctatt ttcgatttaa acagttcaat caataaaaat ataaaaagca
    13141 gaagataaaa tcaatcaggg ttggttttat cttctatttt tattttttta gcatattaaa
    13201 aaaataaccg tattatcgac tgactacact gatgtgctgc ttttcatgtt tctcatccag
    13261 tatctcatct actaatgcaa tcgcgtaatc tgccatgctg atcgtactgt cgctagcagc
    13321 gtttctttgt aaataatcat cacttaacaa ataagtacct gtacgtttgc catgtggatc
    13381 aaataaagaa gctggactga tataagtcca atgtagattt ttagattgtt tcaattttaa
    13441 caatccttcg tacatattga aagctgttgg atagaagggt gcatttttag gtacttggtc
    13501 aatcatacga gtcgtctttt ctgtatctaa gaataacgaa gaggcacccc caacgacaat
    13561 taagcgtgta tcggttcctt caagcatctc aatcaaatgg gacaaagaag tttgatgcat
    13621 ttcttctttt cctttgggag cgttgaaagc atcaacgaca acatcaaaag atgcaagatc
    13681 agtagttgtc aactcaaata aatccttttg tataaacgga acatctgatg gcaatttttc
    13741 tggatgacgc acgattgccg tcacgtctaa atctctggac aatgcttcgt ctaaaataaa
    13801 tgaacctgca tggccagtag ctccaatgag tgcaattttc ataaaaaacc tcctttttta
    13861 ttcctgtcca ttgtaagcag ttgctgaata ttcgtcaaag aatccgattt ttgaaaagct
    13921 cagtgcgcat tcctttcatg gttcgtgatc attgggtata gttaaattaa gaaaactgat
    13981 tgataaaaat agaagttgaa ggagataaga aatgaaagag tggttagatc gtgccttttt
    14041 accaatgata acatctgttg tgaatcttag acccttaact gctttgcgta atggaatgac
    14101 ttacacgatc ccaataatta ttgttggctc gctgtttttc ttatttgctc attttcctgt
    14161 ggattacatc gctcagtgga tagacaaaac aggattttct atgtatttta cacaagtata
    14221 ccaggctaca tttgatgtga tagccttttg gatcgttttt gccattgcat acgcttatgc
    14281 aaaagaagaa gacatcgatc gcttaccagt cggcataaca gccttgtctg cttatctact
    14341 gcttattcag ccactaaata ctcagataga tactgtctgg acaggtgctg aagggatact
    14401 aggagcagtt acaactgggc tcatcatcgg atggggctat gcacgtttag taagacttgc
    14461 gaaagggtgg aagcaaagag agaagatacc tgaaaatgtt tggcagtctt ttacttcatt
    14521 gattccaatc attgttatag tcacttttac gcttatcggt tcagcggttt ttcaacattt
    14581 gttcagaatg actgttgttc aaggagtatg gacatatgtc cagcttccca ttcaatcgtt
    14641 catagacacg ttgtttggtg tgcttttatt aggttttttt gttccgtttt tatggttatt
    14701 tggtcttcat ggatctgcga tggtcaatgg gttggttagt cccgtcttgc aagcgaattc
    14761 attagcaaat gcagaaatct tagcctcagg acaagagttg acggtggcaa atggcggaca
    14821 tatcgttact cagcaatttc tcgatcagtt catgacggtt acgggtgctg gattgacgtt
    14881 aggggctgta ttttttatga cggtttttgc gaaatcgaag aaatacagag aattaggaaa
    14941 tcttgctctt ttaccagcct ttttcaacat caatgaatca attatttttt ccacaccgat
    15001 cgtgatgaac ccgatgatgg ctgttccttt tattttcgca ccggtcgttt caggattgat
    15061 tacctattcc gctttatatt ttggattcgt tcctttattt acagcagtcc aagtcccttg
    15121 gacaacaccg ccgatcctct cgggtttctt agtaggcgga gtgcctgcag cgattttaca
    15181 aggtatcgtg ttgtcaatta gttttttcat ttatttccca tttttgaaaa aaatagattc
    15241 tgcaaactgc aaaaaggaaa gacagacaaa aaatgatgga gcagcagaaa agatgaacga
    15301 ctaaaaaaca aaaatactcc tgtcgcttta gattgggaaa atcaccaatc taggatgaca
    15361 ggagtattta tagtcatgtt tatactagcc ctttttggac agctaattga acaagaacaa
    15421 ctaatgtgtt cattcctacg tgtgtgatca tcgatgtcca gatccgacca gttgctttgt
    15481 actctaggct gaagaagaaa cctaggaaaa agtagattag cagatgacca tcattatgag
    15541 caaacgcaaa cagcaaagag ctgatcactg ctccgaccca aaaatttgaa tatcgactga
    15601 tgatccccag tatactgcgg cggaatacga actcttccat gatgggtcca gcaacagttg
    15661 tcgccaaaat aaaagcgggt gcttccatga tcatctggat gatattttgt gtattctcgg
    15721 aaggggttgc tttgccaaac aatacagatt ctaacatgac agcgatgctt tgcagaatca
    15781 atgcgacaaa gattccaacc aatccataaa taacaatctt tggagcagaa gcttttttta
    15841 ccgctgattc gatcgctaag ggttcacgtt gcttaaaata aaaaatagct aggacaacgg
    15901 cgcctagtat atacatgact gcagttgctg tgatcaattc agacgtagat tttacaagtc
    15961 ctatcgaagc taggatcaag ggtgagataa aaattagacc atagcaaaaa atgctgagga
    16021 agctatattt ttttaaagac atgtaattcc tccttttaag ggataacaag catgcaaggc
    16081 aagcttatat cgattgattt caattatatc ataatttcaa taagcagctg tcagaataaa
    16141 atttcttttc tttgatttaa aagcttgcaa aaaagaggga gaatgagtat tattaaaaat
    16201 gtaggttagc actcgaaggt taagagtgct aacagattaa aaaaagagtt atcggaggga
    16261 tttatcgtgt taaaaccatt aggtgatcgc gtcattatcg aagtcgcaaa agaagaagaa
    16321 aaaactgtcg gaggaatcgt tttagcttct gctgctaaag agaaacccca aactgggaca
    16381 gttgttgcag tcggtgaagg ccgtttgtta gaaaacggtg aaaaagttcc tgccgctgta
    16441 aaagcaggcg accaagttat gttcgaaaaa tacgccggta cagaagtaaa atacgaagga
    16501 aaagaatatc tgatcgtcgc aggaaaagat atcatggcga ttgttgagta aatttggaag
    16561 ataaaaaaat aatcaaaaat aatcaatgag gtgaatcaat catggcaaaa gaattgaaat
    16621 ttgcagaaga cgcacgcgca gctatgttac gcggggtaga taaattagca gatacagtga
    16681 aagtaacatt aggacctaaa ggccgaaatg tcgtactaga aaaatcttac ggctctccat
    16741 tgatcacaaa tgatggtgtg acgatcgcaa aagaaatcga attagaagat cattttgaaa
    16801 acatgggtgc aaaacttgta tcagaagtag catcaaaaac aaatgatatt gcaggagacg
    16861 gtacaacgac agcaactgtt ctgacacaag caatcgttcg cgaaggttta aaaaacgtaa
    16921 ctgcaggtgc taatcctcta ggcattcgtc gcgggatcga attggcaaca aaagcagcag
    16981 tcgaagaatt gcataatatc tcaactgttg ttgattcaaa agaagccatt gcacaagtag
    17041 cagctgtttc ttcgggttct gataaagttg gacatttgat tgctgatgca atggaaaaag
    17101 ttggaaacga cggtgtcatc acaatcgaag aatcaaaagg gatcgaaaca gaactagatg
    17161 ttgtagaagg tatgcagttt gatcgtggtt acttatcaca atatatggta acagataacg
    17221 acaaaatgga agctgttcta gaaaacccat atatcttgat cacagacaaa aaaatctcta
    17281 acatccaaga tattctgcca ttgttagaac aaatcttgca acaatcacgt ccattgttga
    17341 ttattgctga cgatgtagat ggtgaagcat tgccaactct tgtattgaac aaaatccgtg
    17401 gaacattcaa cgtagtagct gtaaaagctc ctggtttcgg cgatcgtcgt aaagcaatgc
    17461 ttgaagatat cgctattttg acaggtggta cagtaattac tgacgatcta ggtttagaat
    17521 tgaaagatgc aacaatcgaa aacttaggaa atgcttctaa agttgttgta gataaagaca
    17581 atacaacgat cgtagaaggt tctggagaaa aagaagcaat cgaagctcgt gtgcaattga
    17641 tcaaaaacca aattgctgaa acaacttctg attttgaccg cgaaaaatta caagaacgct
    17701 tagctaaatt agcaggtggt gtagctgttg ttaaagttgg tgcagctact gaaacagaat
    17761 tgaaagaatt aaaattacgc attgaagatg cattgaatgc tacacgtgcc gcagtagaag
    17821 aaggaatggt ctcaggtggt ggtacagctc tagtgaacgt tatcagcaaa gtttctgctg
    17881 tagaagcaga aggagacgtg gcaacaggta tcaagatcgt tgttcgtgct ttagaagaac
    17941 caattcgtca aatcgctgaa aacgctggtt atgaaggatc agtgattgtt gataaattga
    18001 agaacgtgga actaggcact ggtttcaatg ctgctactgg agaatgggta aacatggttg
    18061 aagcaggtat cgtagaccca actaaagtaa cacgttctgc cttgcaaaat gccgcttccg
    18121 tttctgcctt gttattaacg acagaagcag tcgttgcaga taaaccagaa ccagcagcgc
    18181 cagcagctcc tgcaatggat ccatcaatga tgggcggtat gatgtagtaa ttaagaaaga
    18241 accctatttt aacagggttc tttcttcttt ttttgtttta ttttaagcaa aaggggcaaa
    18301 gaaggggcaa aaatatcaaa ggctatttag tttatcaatt acttgttgtt tggctctttt
    18361 tgttacatgg ttataaatag acagagttgt gtttgcatca gaatgcccta cacgctccat
    18421 aatggcttta agaggtacgc ctaattcaga taatagtgaa acgtggctat gtctgaatat
    18481 atgtgaggat agactttttt ctaattccaa ctcttcctct actttatgga gtatagcgtt
    18541 aaatgagtgc agcgcaagcg gagtgccact tgtagatata aatatatatt gatcgggatc
    18601 tgtgggtcta cctgcaagaa tattgtcagc tatcacactt tcaatcaatt cttttgcacg
    18661 attgggtaat tgcacttcac gttgcgaata agtatttttt ggagttgttt ttatagcatt
    18721 atccattttc acagatgtgt aatctaaggt cccattaatg gaaattttcc catcctcata
    18781 gtccttcatt tgcaaagcta gcaattcccc atatctcaaa ccagttaaat atagaaactc
    18841 agctattatg ccgtgtagtt ttctgcgagg attggagtat agctgtttca gtatttgatc
    18901 aatttcttct ttctccagat attttttatt catagaaagc cttctttttt cttcttccac
    18961 ttttttagga tggattttaa ctgctagcgc agggtttctt tgaatgtatt ttctatcgat
    19021 tgcatagttt agcataacag atagagttgt ttttgtttgt tttgtgtagt tcagtgagag
    19081 gtcaccaaac gtatacatat cttcaattat cttattaatg agtgtctcat caatgtttct
    19141 aacgatcgta tcatcgctta tgtgcttaga aacatgtttc atcatcattg gaaccttcaa
    19201 atagctagta cgtttaacat gctgcttata atattcatac cattctttat acagctcacc
    19261 aaaagtgata tctgatttat tgtaatcttc gagtgcttct ttaatttttt tatctagaat
    19321 tttctgagct tttttccacg cttgtggtga attacttgta agtgttgtag atttttttcg
    19381 tgttttttct gtataaggat ctacatatct ttcaataaac ttgaattggc catctttggt
    19441 ctgttcaacc cacactttta acatctccta tcatttgcta taataggcat aacaaataga
    19501 cctatatagg tttgttttct aaaagcacgc tcttactctg gacggtgggg cgtgtttttt
    19561 atttattata cagttgcttt aaaaatagct gctttttcaa atgatactaa aggagagaag
    19621 tggatttcta tttctccctt ttggtttaac gtgaaatgag cagttacatc catttgtttt
    19681 cctggagcaa cagatcccat tgtattttcg ttggcatatg tctctgattt tttgtcatct
    19741 ggtccatata cttccacatc tgtacctaca gggatatctg aatcgccatc attttttacg
    19801 gtataagtaa tttttactac ttgtgcaggt tggttttctt caaattgatt tctttcatca
    19861 gttagttcta cactatttag tgtatattca gcatcaccaa cttttacagt gtcaccaatc
    19921 ttatagaagg tatcgctttt ttcttcttta gaagatgaag cagaggttga agatttagtt
    19981 actttttcgc caccattatc acttgcttta ttactatctg atccaccatt taaagcagaa
    20041 ccaataatta taattaaaat tactgctaat acccaaaacc atactttttt gtaaaaaggt
    20101 ttactaactt tatacatttt tccgtcttga cccataactt tttttgccat ttaaatattc
    20161 ctcacttctt gttataatat atttgcgatc tcagaaatga ggtatgagtc cgtgtg
//
LOCUS       DACDXE010000056        20211 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.056, whole genome shotgun sequence.
ACCESSION   DACDXE010000056 DACDXE010000000
VERSION     DACDXE010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.056"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..2058)
                     /locus_tag="IWM23_001993"
     CDS             complement(151..2058)
                     /locus_tag="IWM23_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAP9884607.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            2188..3063
                     /locus_tag="IWM23_001994"
     CDS             2188..3063
                     /locus_tag="IWM23_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAP9884608.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            3143..4117
                     /locus_tag="IWM23_001995"
     CDS             3143..4117
                     /locus_tag="IWM23_001995"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAP9884609.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            4306..5442
                     /locus_tag="IWM23_001996"
     CDS             4306..5442
                     /locus_tag="IWM23_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9884610.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            5579..5923
                     /locus_tag="IWM23_001997"
     CDS             5579..5923
                     /locus_tag="IWM23_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAP9884611.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            6194..7480
                     /gene="efmA"
                     /locus_tag="IWM23_001998"
     CDS             6194..7480
                     /gene="efmA"
                     /locus_tag="IWM23_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAP9884612.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(7618..8217)
                     /locus_tag="IWM23_001999"
     CDS             complement(7618..8217)
                     /locus_tag="IWM23_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAP9884613.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            8621..9742
                     /gene="mnmA"
                     /locus_tag="IWM23_002000"
     CDS             8621..9742
                     /gene="mnmA"
                     /locus_tag="IWM23_002000"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAP9884614.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            9988..11409
                     /gene="arcD"
                     /locus_tag="IWM23_002001"
     CDS             9988..11409
                     /gene="arcD"
                     /locus_tag="IWM23_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAP9884615.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            11423..12541
                     /locus_tag="IWM23_002002"
     CDS             11423..12541
                     /locus_tag="IWM23_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAP9884616.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(12601..13893)
                     /locus_tag="IWM23_002003"
                     /pseudo
     CDS             complement(12601..13893)
                     /locus_tag="IWM23_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
     gene            14043..14603
                     /locus_tag="IWM23_002004"
     CDS             14043..14603
                     /locus_tag="IWM23_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9884617.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            14732..15109
                     /locus_tag="IWM23_002005"
     CDS             14732..15109
                     /locus_tag="IWM23_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAP9884618.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            15042..17114
                     /locus_tag="IWM23_002006"
     CDS             15042..17114
                     /locus_tag="IWM23_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9884619.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17325..18272)
                     /locus_tag="IWM23_002007"
     CDS             complement(17325..18272)
                     /locus_tag="IWM23_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9884620.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            18518..18982
                     /locus_tag="IWM23_002008"
     CDS             18518..18982
                     /locus_tag="IWM23_002008"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAP9884621.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(19044..20087)
                     /locus_tag="IWM23_002009"
     CDS             complement(19044..20087)
                     /locus_tag="IWM23_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAP9884622.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
BASE COUNT         6491 a         3708 c         4239 g         5773 t
ORIGIN      
        1 gaaactaagc agctttttca caacctctaa tacatggtat tccagaagta acttcttcga
       61 aaaataagcc gaaatgtcac aaaaatttga gaagcgattt tcccaaattc cttcttattt
      121 cttgaagctg accacttctg gcacaacctc ttaccagatt cgcactcttt tttctggcgc
      181 tagatacata gcgtcttgtt ccgtgatacc aaatgtttca tagaattcgt caaggttttt
      241 tggttgcacg tttgctcgga gtttagcagg ggcatggaca tcgatttgca gtaaaagttg
      301 ttggtattct ttttttgctt ttgtacgcca aatcgttgcc caattgacaa agaattcctc
      361 gatagaaaga tctgattccg tttttgctgc ttccaatgca cagcttagac cgcctgcatc
      421 agcaatattt tccgaaacgg ttaattttcc gtttactttt ccgtcagcaa aaggcagacc
      481 atcgaattct tcgatcattg cttgtgcttt gttttgaaat tgtgtaagat cttcttctgt
      541 ccaccagttg ttcaagttgc catattcgtc aaataaagcg ccgttattat caaatgcatg
      601 ggaaatctca tgagcgataa cagctccgat tccaccataa ttagcagagc ttgattgttc
      661 tagactgtaa aatggtgctt gaaggatcgc tgccggaaag acgatcacat tccgaaatgg
      721 atgataataa gcattgaccg tgtcagcact catttcccat tctgaacgat ccactggttt
      781 gttccatttg ctgaaacgat cttttaatgt gagtttgctg aaaacaagag tatttgaaag
      841 caaagtacca ccctgatctg caggtactgt tttgaactgg tcgaataatg ctgggatctt
      901 atcggggtag cctacttgga tacctaagtt atttaatttt atgacggctt tttcttttgt
      961 tgcatcccca agccagtcat tttcttctag acgtttttta taaacggcga tcattttttt
     1021 caccatttgt tctacgtctt ttttagcagc ttcgccaaaa tatttttttc cgtagtaatc
     1081 gccaactact tgatcgaatt gtccagaggc aaggtaatac gctgcttttt tctgaggcat
     1141 tgcttcgtct gttccagaca atacgcgaga atagattccg ctgacttggc ggaattcttc
     1201 tgatagataa ccactaagag aacgtactgt tttaatgatc atccagcttt tgaataacgg
     1261 aaaatgagct tctgtcagga tgtcattcaa atgatcgaag tacactggat cagttacgat
     1321 cactttatct ggagttgtcc caatcaaacc cgtgacaagt tttttcaagt ccagtttatc
     1381 tgtgtattta ataaatgtat caaaagattg cggattatac attttgctgt aatctgcgtt
     1441 ttcttctgca ctttttacat gaggagcaat caatttatca aattgcactg cttgttccgt
     1501 gattgcttct gcctgacttt tttctttgcc ggcaagctct aataactgaa cagccatatc
     1561 aaagaaaacg cctattagtt gtgcaccgtt gggatggtct gatgcataat acgtcttatc
     1621 tggaaggaat aaagaaggcg gataagcaaa gaaggcgttc gtttgcgcat tcttcatatc
     1681 tgcatctaca tccagattaa atggcaatgg taatgtatcc aacagccatt ctggaaattg
     1741 cttattcaat tcgtcgaaag actgcaatga ttcaactttt tcgattatag gttgaagcgg
     1801 ttgagcatgc aactcatttc ttcggtcata atcgtttgcc agttgataat atgcaaggaa
     1861 atgtttcatt ttttctgaag acactttttc tggatgggca agcagatcgt ctgtttctcc
     1921 catcaaaagt tggtcgatcc cgtcaactaa atcttggaaa ccgcctgtag ctggtttatc
     1981 tgcagggatt tttgctgttt tttgccattc ttcattgact gcttcataga aatcttgttt
     2041 caatcgttct ttgttcattc atcgatcgct ccttttttgt ttcgtgtgcc cattatctta
     2101 caaaatgaag gaattaacaa atagaacata aaaaattgaa aattcgaaca atcaaactga
     2161 tttgtttttt tcaatccttc acttataatg aaaaaaagga ggcgatccca actgaatcaa
     2221 gtagtggata aacctttata tttcaaagta gataaaaaaa ttaaaaaatt agccagcact
     2281 cagcagctgc agtcaagaaa aagtaaattg ctttttttag ctctggtttt tgaagatcaa
     2341 agttatgtga ttatcgatca atcaggacat ccggtagaat attctccagc aaaatatact
     2401 tatcaagagg gtctttcttg taaaaaatgg aagctgatca atgaagagcc aatcgagtta
     2461 tctagatgga tcaacagaaa agaagatatt cccgttttga tcgaagaaaa aaggtcagga
     2521 aaggaactcg caaattgctg ggtgggattg ccagaagaac gttttttgag gtataagaaa
     2581 tgggctaccc cctcaggcta tctttgtggt acttacgcgg cagcagttct ccttgcctat
     2641 tatcaagact atcgtaaggg gtggatgctt ccaaatgaga ttcgcaagaa aaatacggct
     2701 gattcgttgg ttttgacgaa ggcattgcgt agccagatcc aaccactagg acttcctacg
     2761 attccttttc aggttagtac agggatttct agttttttaa agaagaatgg caatcatgag
     2821 cgtgcacgag caactcttct aggttcatgg cagcgagcta caaaacgtat acgtgaaggc
     2881 aaacctgtga tgattggtat tttgaaagta cttggcagta catatggcaa tcattgggtg
     2941 acagcgtacg cttattttga aactgaaaca ggagaacgtt attacaaagt acacgacaac
     3001 tggggcgatt atcataaagt cattcctgcc agttggagta acggaacagt ttcacttcct
     3061 taagaatttg ctgaaattaa cagaaaatct tggaaaaata tgttaaacta tcgaagaata
     3121 gagaagtttt ggggaggact acatgagtaa taagtatcag gatgacacgt tgaagatttt
     3181 tagtttgaat ggcaaccgtc cattagctga aaaaatcgcg aaagtgttcg gaacagaatt
     3241 aggtaaaagc gtcgtgaagc aatttagtga tggcgagatt tccatcaata tcgaagaaag
     3301 tattcgaggg gatcatgtgt atatcgttca atcaacgaat gcacctgtca atgattacta
     3361 tatggaacta ctgatcatga tcgatgcaat gaagcgagca agtgcgaaaa cgatcaatgt
     3421 cgtattgcct tactatgggt atgctaggca agaccgcacg gcgaaacctc atgaaccgat
     3481 cactgcgaaa ttgatcgcta acttgatcga agaagcaggg gcaactcgag tactgacttt
     3541 agatctgcac acggttcaag tacaaggatt ctttgatatt cctgtagata atttatttac
     3601 gatgccgttg tttgctcatt attaccgtca attaggtctg acgggagatg acatcgtagt
     3661 tgtttcgcca aaaaacagcg gtgttcagcg tgcacgtagt ctttcagaat atttgaacag
     3721 cacattagcc atcgtagatc atgcagatga tgcttcaact gagggtggtt atgtgatcgg
     3781 tgatgttcaa ggaaagacat gtatcatggt agatgatatc ttgaacacag gagcaacgct
     3841 tgcacgagca gcaaatgtat taaaagaaaa cggcgcaaaa gaagtatatg cctgtgcgtc
     3901 acatggatta ttgtccgatc atgcgaaaag tattttagac aatgcaccaa tcaaagatat
     3961 ttgtatcact gattctgttt tcacagaaga aaatcgtcat ccagaaaact taacaatcgt
     4021 tacatgttca gaattaatgg gtgaagcggt caaacgcatc catgaaaata caccgatgag
     4081 tccgttattc cgtctggaag aaaaagaatt cgaataaaca aaaaaaagag ggaaagaagt
     4141 tgtgacagaa gtgagcagct tcaaggagct gcgacaagac ttttgtcaca gctccttttt
     4201 tctatgccca tgaaacgtcc tctagacaaa aatgagctct tctttggttt gttcgctatt
     4261 ttatgctaga atgtgcctag caataatttg gaagggatga atgaagtgaa aactatttat
     4321 ctggatcatg cggcaactac ccccatgcat ccacaagtaa ttgaagcgat gacggcaatc
     4381 atgcaagaca cgtttggaaa tccttcaagc atacatggat ttggtcgcca cgcgcatgaa
     4441 aaattggaaa cagcaagaca aatcattgca gacagtctgc aagcaaaacc tcacgagatc
     4501 atctttaaca gtgggggaac agaaggggat aatacagcta tcttagaaac agcgttctcg
     4561 cgtcaaaaag aaggcagaca tctaatcact acagctatcg aacatcctgc tgttcttaat
     4621 accatgaaat atttagagac aaaggggttt gacgttacat atctgcctgt agatgaaaaa
     4681 gggaatctgt cgatcgaaga ggtcaaaaaa gcgttacgag aagatacgat cttggtctct
     4741 gtgatgtacg ggaacaatga aatcggaaat ttgatgccaa tcaaagaaat cggtgaactt
     4801 ttagcggatc atccagctta tttccataca gatgcggtac aagcgtacgg aaaccaaacg
     4861 atccatccag aagaattagg aatcgattta ttaagtatct ctgctcataa aatcaatggg
     4921 ccaaaaggcg tcggtttctt atataaacga gacggagtaa atatccctgc attgctttta
     4981 ggcggggagc aggaagaaaa gcggcgtgca ggtacagaaa atttagcagg gatctggggt
     5041 atggcaaaag cagttgaact gctgtcacct gaagaacgtg aagcacgtaa caacaaatat
     5101 aaaggattcc agcaattgat tcttgataca ttagaagcaa acgaaatccc ttatcaagta
     5161 aatggtgatt taacgaataa actccctcat gtgttgaatc ttcgcttaat tggcgaagca
     5221 aatgatttgc tactgatgaa gcttgatctt aatggggtcg ctatttctac tggttcagct
     5281 tgtacagcag gaaatatcga gccttcacac gtattagaag caatgtatgg agaagataca
     5341 ccgatactaa aagaatccat ccgtatcagc tttggtttgg gaaataccga agacgacatc
     5401 cagtatttcc ttgatcaatt agttcgtgct gtcaaaaaat aactagtgaa gtcattctta
     5461 tatgaacaga tgtctgagag aacgccttca ttttttaggg aacttaactg aaaaacctag
     5521 taaaaatcag gataaacttt tataataaag agaaactaat tttatcgagg tgaaaatcat
     5581 ggcatttgaa acaacagcaa cggtactggg cgcaccaatt agctatcgtt tgcatcctga
     5641 tgcaaaaaga tatacattgc gcgataatgg atttacagaa acaaacggag gcaattatca
     5701 attgatccga ccattagatg ccacgccgca aagcaaagaa ggattcaaat taaaaatcac
     5761 tgtatcaaaa gatattcaaa cattgaaaat gtcgatcact acagcaaatg gattgaaagc
     5821 agtcaatatt ttcaaagatc caaaacagaa aatgagccaa gataaatttt attttctgat
     5881 ggatgggatg attagccgtg ggttattcga aaaagtcgaa tgatctgtgg atataaaaag
     5941 acaactagtt ttgaaacggc ctattgttag atgactaact ttgtaaggac gacgcaaatt
     6001 agttgtcttt tttattttta aatttaacac atgttgttat ttttaatatt ttgctgattt
     6061 attgtttcta agtaaagaaa tttaaattga aacgattgtt atttagggaa aaaggtattt
     6121 tttgaaaaaa gaatcccgta taatgattgt tgtgcaaacg ctatacaaaa tgaaaaagaa
     6181 aagagggatg tttatggaaa atgaacagtc ggtcgtttta acgaattgga agagaaatta
     6241 tttgttcttt ttatcgggac agtttttgtc aggaatcact agtatggtcg tccaatatgc
     6301 gatcatctgg tatttgacaa gagaaaccgg ttcggcgaca attttgagtt ttgctacttt
     6361 actaggaatg attcccatgg ttttactaag cccatttgta ggaccgctag tggatcgctg
     6421 ggataaaaaa gctcttttga ttgtcacgga tattattgta gcgattttcg cgcttatttt
     6481 agcagtagtc ggaacgattt cagaatcctt cccgatttgg ctagtttttg tgtctttgtt
     6541 tatgcgttcg gtcgcacaaa cattccaaat gccaacgatc cagtcgatta tgccgacaat
     6601 cgttccatct tctcacataa caaggacgaa tggacagtta ggaatggtcc aatcagcgaa
     6661 tttcatcatt gcgccagctc taggtgcggc tttgttttca gtcgttcctg tcaattacct
     6721 tattttatta gatgtattgg gggcagtttt tggtgtcggc ttattgattt ttgtgaagat
     6781 cccaaaagtt tctcctgaaa tattggaagt tccgcttact atttttaaag atgcaaaatt
     6841 tgggctgcag caattgatgg ataataaagg gttatggtac ataacgatca atggtgcatt
     6901 tgtgatgctg ttgttcatgc cagcaatcag tctgtatcca ttgatgacgc tagattactt
     6961 tggtggttcc gttggacagg cgggagcagt ggaagtagtc tacgcagtcg gaatgctctt
     7021 aggcggtgca ctgatcagtt ttataggaac atggaaagac cgaatgaaac ctattattat
     7081 agcctacatc attatgggcc tgacgatcgg tgcaagcgga ttggttccaa acgatagtca
     7141 agggttcttg tactttctta tcttgaatgc aggagcaggt tgtgcaacgc catatttcaa
     7201 tacactgctg atggcaatga tccagcagag ttatgaatcg aatgttttag gtcgtgtctt
     7261 agggaacttc aactctttga tgaacctggc aggtccaatt ggattattat ttgcaggacc
     7321 attagctgat cgtttagggg tagaaaaaat gttcttgttt tccggaatcg ggattttatt
     7381 atgcggaatc gttttatttt taacgtcagc tgcccgaaat tatgataaag aattacagaa
     7441 aaaactggta aaagaacacc atgagcaaaa agacgaatag tatggaatgg agttgtgaca
     7501 gaagtcttgt tacaactcca tttctgatcc taatgttcct gtggcaataa gcctactcaa
     7561 atgaaaaata tgagtaatta tctttatttg tttaatactt gatatagggc agttttttca
     7621 cgacctatcg tacttcctct ttgcttcata gatttctttg atttgttgct cagataacgg
     7681 agtgccaaat ggtagttttt cctttaagag ttctctttcc ggtgcgtcta cgcctacatt
     7741 gacatcgttc tcgataggta cgctgtaatg atggatatgt ccatgaagat tgcggatagt
     7801 gtcgttaggc ccgagcaaca tcggataatg agtcaagtaa tattgttgat ggtcgaattt
     7861 aacgatggca cctacatcat aaaaataaaa tttgggactt ccgtcagtaa gcctaggatc
     7921 atttttttct aaataacgga aaaaagcgcg actgtcatgg ttccctttga tcaagtggat
     7981 tgttccttgc agttgagtga ccagttccaa aatttctgca tttgcttttt catattgcgg
     8041 atgcatagca aggtcgccta gatgataaac atggtcggtt tcttttatta ctgcattcca
     8101 gctagtgatc aactgttggt tcatttcatc cacatttgca aagagccgcg gagcaaagtc
     8161 gttttttcct aataagcgtt catggaaaaa gtgcatatca ctgataaagt aattcatgta
     8221 tcttttgcct cccttttttc ttagagtaaa caaaatcact tatctgagca actcataaca
     8281 tctaaaaaga aaaggttgta aatgagacgt gcagctttaa gcgataagaa gcaattttga
     8341 aaaatagttt ttcacaattt ataaaattat tccttattgc cgaaaggctg gcccttttta
     8401 tgagtatgcc aattttttat atcgaatgct caaaagatga gtgatttttt ggttgactat
     8461 catgtttgtg tactgaatag gtccagtaga acagtatttt tacgacttga taattaaggg
     8521 atgaaatttt cagaaatatg acgtataatg taaaatactg aaaagttgcg accttcaact
     8581 cgtagatgtt tcagaatcta attgaaatga tggtgatatc atgacagaca acagcaaaac
     8641 acgcgttgtc gtagggatga gcggcggtgt cgactcatct gtaacagcac ttttgttaaa
     8701 ggaacaagga tacgacgtga tcggaatatt catgaaaaat tgggatgaca cggacgaaaa
     8761 cggtgtatgc acggccacgg aagattataa ggatgtagcg aaagtagctg ctcaaatcgg
     8821 gattccttat tactctgtga attttgaaaa agaatattgg gatcgtgtat ttgaatattt
     8881 cctagccgaa taccgcgcag gacgtacacc taacccagac gtgatgtgca acaaagaaat
     8941 caaattcaaa gcattcttgg attatgcaat ggatcttggc gccgattatg tagctactgg
     9001 acattatgcg caagtcactc gcgatgaaaa cggcgtggtc cacatgcttc gtggagtaga
     9061 caataataaa gatcaaactt acttccttag ccaattatca caagaacagc tttcaaaaac
     9121 gatgttccct ctaggcggca tggaaaaatc agaagtacgg gcaattgctg aacgcgcagg
     9181 ccttgcgact gctaagaaaa aagattcaac gggaatctgc tttattggtg aaaagaactt
     9241 caaacaattt ttgagcaact atctgccagc aaaaaaagga aatatggtca ctcttgatgg
     9301 tgaagtcaaa ggacaacatg ctggcttgat gtattatacg attggtcaac gtcaaggact
     9361 agggatcggt ggcggaggag attcgcaaga accctggttt gttgtaggaa aagatctagc
     9421 gacaaacaca ttatatgtgg ggcaagggtt ccatcatcct gcattgtatg ccacaagctt
     9481 ggatgcaagt gaaatccact ttacgacgaa tgaaccaatg ccaaaagaat tcaagtgtac
     9541 agcgaaattc cgttatcgtc agcaagacgt tcctgtgaca gttcgcttat tggatgataa
     9601 tcgtgcagaa gtcgtattcg acgaacctgt acgtgcaatc actcctggac aagcagttgt
     9661 attttatgat ggaatggaat gtctgggtgg tggtttgatc gaccacgcat atcaagaaac
     9721 aaaagtttta caatacgttt aagcgatatt aaatgaatca aaacagctat ggctaacaaa
     9781 agaaacacct tttcttttgg atagcatagc tgtttttttg caataaaatt aatttttatt
     9841 cattttatac ttgaaatact tgtgtaaaaa gttcataata tccaataata tgaataaagt
     9901 tgctttttgt taagcaagag cagttgcaaa atagaaggga atcttttgca agtgatttgt
     9961 tcaattttat aagtgagggt gatggaaatg gaaaagaaat cgacgggtat tagcaaatgg
    10021 ggactggttg ctctggttgt cagttcgtca atcggaagcg gtgtgtttgg gatcacaagt
    10081 gatctggcta gttctgcagc tccagggcca gcgatccttt catggataat cgttgggatt
    10141 ggtattttag ctttggtatt atcgctgaat catttaggag aaaagcgtcc agatctagat
    10201 ggtggtattt tcggttacgc ggaagcttca tttggtaaat taggtggctt catcagtggt
    10261 tggggttact ggttgtctgc gtggctggga aatgttgctt ttgcaacgat gctgatgagc
    10321 gccatgggag aatttttccc gatattcaaa ggtggacaaa atgttccttc tattttattt
    10381 gcaagtattt ttatttgggg tctgacttta ttagtaaaca atggagttga aggagcgagc
    10441 gtcatcaata caatcgtgac gatctgtaaa ttagtgccat tattcctatt tttaatattt
    10501 gtatttatcg catttaaagt gaatatattc actgctgatt tttggggtaa cgtatctgat
    10561 aatctagtga atggaacagg gcagcaagga acgatctggt tgcagattaa aggttgtttg
    10621 atggttatga tgtgggtctt tgtagggatc gaaggcgcaa gtgttttagc caaccgcgcg
    10681 aagaaacgtt cagaagccca gcaagcatcg atcattggtc tgctttgttt attgtttatc
    10741 tatatccttg cttcgatctt gccatacggc gtcttatctc aagaagagtt agcaactatt
    10801 tcacaacctg ctatggcaaa tattttaaaa ggaatcgtgg gaaattgggg agcagcgctg
    10861 atcaatatcg gcttgattat ttcgatcatt ggttcttggt tgtcttggac gatgctccca
    10921 gcagaaacaa cgatgttgat ggctcgagac ggtctgttgc cgaaaggttg gggaaaaacg
    10981 aataaaaaaa atgccccaac ttattcgtta gttgttactg caatcttgac taatctattc
    11041 ttattgactt tcttagttac tgattatgcc tatcaatttg cctattcttt atgtacagcg
    11101 gcaattttga tttgttatct acttgtcggg atctatcaag tccagttttc tttaaaagaa
    11161 ggaatcaaaa aacaagtagt gatcggtggg attgctgtag tttttgaatt gatgggcatc
    11221 attttagcag gattttctta tgtcctttta tgtagtattg cctatcttcc tggtttttgc
    11281 ttctactgga tcgcttgtcg tgaaaccaaa catcaaatta cgggaaaaga aaaagtaatg
    11341 attgggctga tttgtgtggc cgcactgctt tcaatcgggt tgcttgctgt aggctggatc
    11401 aagatataag ggggaaagaa acatgaaaat cgcaaatttc ggtgtagaag aatggctaaa
    11461 taaatgggaa aagaaagcgg tatatgatat tgcacaaagc tcgatcgaag cattgacatt
    11521 agacgaactc gttggcttag atgggactag cgtaagtaaa tttttcgaaa aacgcaaaaa
    11581 cgaaccattg gactatggct ggatcgaagg atcagatgtt ttcaaacaag aagtcgcttc
    11641 tctttatcaa accgtcgatc cggaaaacat cttacaaacg aatggagcga ctggtgctaa
    11701 tcttttagct ctatacgcgt tagtggaagc aggagaccat gttgtttcga tgcttcctac
    11761 ttatcagcaa ttatatgaca tccccaaatc tttaggtgcc gctgttgact ttgttcattt
    11821 gaaggaagaa gaagattggc agtttgattt agaacagcta gaagccttag tcaaacctga
    11881 aacaaaaatc atctgtttga atagtgcaaa caatcctaca ggcacattgc tagatcggaa
    11941 aactttagaa aaaatcgcag aaattgctcg tacagtagat gcatatgtat tgatagatga
    12001 agtatatgcg cctttaacgg ataaaggtga attcttgtcg atcgtagatg tctatgaaaa
    12061 aggtatagca accaattccc tatccaaaac ctactccatt ccaggtattc gtattggctg
    12121 gacagctact ggacctgagt tggcagaagt ttttcgaaag tatcgagact acacgatgat
    12181 ttgcggcgga gtcctgtcag atgatcttgc tgtacatgca ttgaagaata aagaaaaaat
    12241 attggcgcga aacaaaaaga tcattacaga gaaccttgtg attttaaaac aatgggtagc
    12301 caatgaaccg aaagttgaac ttgtcgctcc caattacgtc tctacttctt tcattaaatt
    12361 agccatcgaa gaagatgacc agacattttg tatcaattta ttagaagaaa caggagtact
    12421 attagtacca ggaagtgcct ttgatttacc gaagcatgcc cgactaggtt actgttgtaa
    12481 aaaagaaacc ttggaaaagg gattattcct cttatctgag ttcttgaaaa aacaggccta
    12541 aacaaaaaca ctgcaaggac agtctgtgac aactgttcaa gcagtgtttt tcatttacgc
    12601 ttattgtcta tgagaaggag agtgccctga agctatggag actcctgaag ctttttcttc
    12661 tgcagtttct tgctgatgga tctcttgtgt attgtgtcgt acaagcaaga agttgaccaa
    12721 aacaagaaga gaagtcgaaa gaaagacgcc gcgataacca aatgcactgg aaacactgga
    12781 gccgatcata ggccctacta cgttaccagt tgcttggaaa gattggttat aactgaagat
    12841 ccgtccagca gcttgatgag gcgaatattt tgtgatcaat gcttggacag cagggagcag
    12901 acaagcatca gagataccaa tcaaaaaacg taaggcagct aattgccaaa cattcgtgac
    12961 gaatgccatt gggatataaa ccagaatcgc aaaacccaaa ccaatcgcaa gtattctttc
    13021 actcccaatc ttgtcaccta agcgaccaaa tcttggagcg gctatcaaag tggcaatccc
    13081 aggtatcgaa gcaatgattc cgctgatcaa cgtaacattc ccatgtccat gcagcaattg
    13141 gcgaatgtac agactgatga ttggactaat ggaattgttg gatgcttgga tgatcatcgt
    13201 tgtgacgaac ataccgatga cgacgtgtgg atatttcaag tctttgaaaa tctgtttggc
    13261 agaaaccatg ttttcttttt tcactgggac aaatttttca tggacgaaaa acagactcaa
    13321 caagaagacg aaaaataaga tgatccctgt aatgaaaaag gtagggcgat agccaaacgc
    13381 tgaagcagag atcccgccaa gaagcggtcc aagtaatgtt cctgtaacag aaccagttgc
    13441 taaggttcaa agaacttgac cacttttttc ttttggcgtc ccagttgcga caagggcagt
    13501 ggcattactg atataaccag aaaaaatccc ttgaagcaga cgcaatgcga ctaattgata
    13561 cacactagtc acgcaaccca tcaaagaaat gacgattgcc attcctaatg aagcacgcaa
    13621 cagcatcaat tttcttcctt tttggtcggc taattttccc caccaaggag agataagcgt
    13681 agtcaccaag aaagtagatg aaaaagtcaa tccgctccaa aagttcaatt gtgacgtact
    13741 ataattacct aacgtatcta tgtaaagtga cataaaaggc atgactaaac taaatccgat
    13801 accagccata aatgttccaa accataagac aaacaagttt tgttcccaag tttctctttt
    13861 cctaaataat ttttccttta ttcttgcagc caattagact ccttctttct gaaaattttc
    13921 atctacagct ctttattata aactacaata tgtagtttat caatgaaagt ctcttttttc
    13981 tctttctttt tcgtggtaaa ctgaaagagt gaaggattta ttttatgaga ggaaacatgg
    14041 aaatgaaaaa aagaaatctg tctcaagaga aaatcatcga ctgctttcga gaactagcag
    14101 aagagatgga tgttcagcaa ataacctttc agcatttagc caaacgcttg gatatcaagg
    14161 ctccctcgct gtataaccat ttcaaaaata ttcgtgatgt caaaacagcg ctaacagcga
    14221 aacttctgca ggaacttaat gaccagctgc gacgggctct tgtgggaaaa agtggtcagg
    14281 aagcactgca gatctatgca gaaacttatc gatcctttgc tttttcaaat caagcagtgt
    14341 atgagttact gatcagtgta ccgcatacca atgaagctgt tttattagat ggaatccatg
    14401 aaacgaatca gatcattttg cagatatttg aggcttttca attaacgaaa gaagaaaaag
    14461 ttcatcgaag cagggaactt cgtagtatga tccacggtta tctttcctta cgttttttag
    14521 gatactttac aaaagaacca acaatcgatc cagaagacag ctatttttgg atgatcaata
    14581 actttatcgc tactttgcct taaacaatca gattcatttg taacaatttt aagatgttca
    14641 ttaaatgacc cttttataag agaaaattct tgcaactttc ttgaaaagtg actaagatga
    14701 gtctagaaaa gctgaaagaa ggagcacgag tatgtatcaa gtaattacca tgttcgggga
    14761 caatgagcct tggtggtttt ttgaagactg ggaagaagat attgaagaag aagaaacatt
    14821 cgagtctttc gaagaagcac gacaagcgta cgaaaaacaa tggaacgaaa tccatcaaaa
    14881 ttatgaatat atcaatgcaa aaagtaactt tctcagcgcc ttttggaatg acggagatga
    14941 gcgctggtgt gaagagtgtg acgatgattt acagcaatac aagggacttg cgttgttgaa
    15001 aaatcatcag cccatcacag tagaaagcag aaaggaattt tatgaaacaa ctaattctag
    15061 cggaaaaacc aagcgttgcg aaagacctaa gcaaggtgct tggtgctaat caaaagcaca
    15121 aaaattatta tgaaggaccc aaggtgatcg ttacttgggc ccttggtcat ttattaggat
    15181 tgaaaatgcc ggaagatctg aataaggaat ggcagagctg gcaaatggac acgttgccaa
    15241 tgatccctaa aaaactagga atcaaaccac ttcctaaaac aggacatcaa ttaaaagcaa
    15301 tcaagcagct ggctcagcga aaagatgtct cagaagcagt cattgcgaca gatgccggaa
    15361 gagaagggga acttgttgct cgctggatct tagagtatgt gcatttcaat aaaccagtga
    15421 agcggttatg gatctcttct caaacagata aagcaatcaa aactggattc aagcaactaa
    15481 gacctgcgaa agaatatgat gcattgtatg aatctgctct tgcccgggca aaagcggact
    15541 ggctggtagg attgaatgtg accagagcct taactgtgaa gtacgcggat aatttatcag
    15601 caggccgtgt gcagacaccg acactagcat tagtgagaga ccaagaaaag aaaatcgagc
    15661 agttccgtcc acaaacgtat ttcacgatca cgttgcaagc tggaaaagaa caagccaaaa
    15721 tgcttcaaaa aaatcaattt gctttaaaaa cacaagaaga agcacaaaaa cttgttcaat
    15781 tgatcagtac aagcaaagga actgtcacag aagtccaaga aaaattaaaa acggaatctg
    15841 cgccattgcc ttatgattta acagagatcc aaagagaagc caatcaacgc tatggctatt
    15901 ctgcaaagaa aacattaagt cttgtacaaa gtttgtatga gacccataag atcgttactt
    15961 atccgcgtac agacagcaaa tatctgacaa atgacatgaa aagtacgatg aaagaacgat
    16021 tgcaagcagt cagcgacttt gctcctgagg tcaagcagta tttgaaaaac ggagccatcg
    16081 tccaacagac aaaagtattc caagacagta aagtgaccga tcaccatgca cttttgccaa
    16141 ctgaaaacag agcacgttat gaaaaattaa gtaatgaaga gcgaaaaatc tatcaaatga
    16201 ttgtctctcg ttttttagga ctgtttgctc aaccccataa agtcagccaa acaaaagtta
    16261 cggttgaatt tggtaaagaa cgattcattt ttcggcaaaa tcgtgtcgtc caagcaggat
    16321 ggaaaggcga aactgaaact gagacggaaa cagtcaaatg ggaaaaagga atgagaatca
    16381 atcccgattt cgccatcaaa aaagaactat ctgcaccgcc taaaccattg tcagaagcaa
    16441 gcttgcttgg tcaaatggaa aaatacagtc tgggaacccc agctacgcgt gcagagatta
    16501 tcgagaaact aatcaaatct gaactgatgg aacgtacccc tcaaggactg caagtatctc
    16561 ctaaaggtaa gcaattatta gtattggtaa atccctcttt agtaacacca gatttgacag
    16621 aaagctggga aaaagacctt gaagctattg ctgcaggaaa gaaacaagca ggaaccttct
    16681 tgaatggaat cgaaaaagaa acaaaacgct tggtgaatga gatcaaatca agcaaacaag
    16741 aatatcaaga tttctcgatt acccaaaaga aatgcccaga atgtggggcc aatctgcgcg
    16801 agaaaaacac acgtgacggc aaaatctatg tctgcaccaa ttcagattgc aactaccgca
    16861 gaagaaaaga tccaaaagtc tctaatcatc gatgcccgca atgtcaccgg aaaatggaaa
    16921 tcatcgaagg taaaaacggt gcttatttcc gttgtaaata cgatggaacg acagaaaaat
    16981 tgcttgataa aaaagagcag aagaagaaaa tgaccaaaca tgaagaacga cgcttgctga
    17041 aaaaatatgc acaagcagaa gaaccagaag aaagcccatt agcagcagca ctaaaagcag
    17101 caatgaagaa gtaataagat atgaaagagg ttgtgataga agtggtcagc ttcaagaaat
    17161 aagaaggaat ttacgaaaat tgcttctcaa atttttgtga aatttcggct tatttccgaa
    17221 gaagctactt ctggaacacc atttattcgg aaaaaaggac tgtgacaaga cttttgtcac
    17281 agtccttttt catttaaaaa aatgtttact tcatttgacg tatattattg gtgaggcttc
    17341 aattcatttg gcttcacttt ttctgtttct cttatgatat aaacaggacg ctgtttcgtt
    17401 tctaagaaga ttttcccaat atatttcccg atgattccaa gacataacaa ttggattcca
    17461 ccaatgaaca ggaagatcga gaccatggaa ggccatcctg atgtaggatc accaaacaac
    17521 aatgcccgca aaataatgaa caaaattgca ataccagaac cgacacaaga caaagcgccg
    17581 ataaatgaag caatcgttaa cggtgcatcc gagaagttca cgataccatc aatcgagtag
    17641 ttgaataatt tccagaagga ccaagatgtt tcgccagcca cacgttcgcg gttttcataa
    17701 gaaatatatt ctgttttaaa tccgacccag ctaaataatc cttttgaaaa tcggttgtat
    17761 tctgttaatt ctaggacggc atccaccatt tgacgagtca tcaagcggaa atcacgagcg
    17821 ccatcgacca tttctgtatc gctgatttta ttgatcaact ggtagaattt tttcgcaaag
    17881 aaactgcgta tcgcaggctc gccttcacga gtcgaacgcc gggtgccgac acaatcgata
    17941 tcagaagtag tgatccggtt gatcatctca ggaagcagtt cgggcggatc ttgcagatca
    18001 acatccatca cggtcactaa atctcctgta gaagcttgca atccagcgta cagagccgct
    18061 tctttcccaa aatttctgga aaatgagaca aagcggacat aaggataagc tgaggctagc
    18121 ttcttgattg ctgctaacgt accgtctttt gatccgtcgt caataaacaa atattcgaac
    18181 tcatgggcac ctttgaattt ttcaacctca tcaaaaaaaa gaggaacagt tttttcttca
    18241 ttaaaacatg ggacaacaat tgatactttc atcgtttcta tcctttcaat cgtatgttta
    18301 tatatggttt acattcaaaa tttaataaac cttaaataac acctttaaaa tttgatttta
    18361 cgtaaaagaa caggtattct ttttgtaaac taacgtattt agtttaccat gtttcctcgg
    18421 tttatcaaac ttttaagaac aatttttatt agttggcaag tagttttgga tagagaaaaa
    18481 ggaggaagtt ccagtctatt gactaggagc aaacaaaatg aaaatacaca cacctatcgg
    18541 ctttttatgg ttagatgcag atgaatccgg attgactgct gtatcttttc agccaatttc
    18601 agaaagcgaa tcagcgaatc agcgaatttt aacgcgcaca caagaacagt ttgaagaata
    18661 ttttgcggga aaaagaaaag aatttgatgt tcctttatct ataaagagag gtacgccttt
    18721 tcagcaaaat gtctggaaag ctttgtcgac cattccttat ggagaaatcc gaacgtataa
    18781 agaagtagcc gttgctgttg atagtccgaa agctgtgcga gcgattgggc aggcaaatcg
    18841 aaccaatcca ttgccaatcg ttattccttg tcatcgagtg atcgggcaaa atggaaaact
    18901 gacaggatat atgggaaacg caatagatgg ggttgaaatc aaacgccaat tgttgttgtt
    18961 agaaggatat ctgcaaaatt aaaaacagtt gtgattcgga cattgttcca tgcgagaatc
    19021 acaactgttt tttcatgttc attctaaact ttgatcagcc aaataaatag taaagccatc
    19081 actgtttgga gaagcccgac agctaatcca taagataaga agcgtgtacc ttctttcatg
    19141 aattttgcaa agtctaaacg cagaccaatt gccgctaaag cagtgatctc aaaccagctg
    19201 ctgataaaat gtgccgattg gtcaataact acaggtaaag aaaacaagct gttgaccaaa
    19261 caaaagatca caaaccctaa tacataccaa ggaagcggta attttcgttt tttctgcgta
    19321 tcagttggcg tttcaaacag agctggttgt ttcttgtgca tgaatttttc aaacagatag
    19381 acaacaacaa cgagcatcat aatacgtaga atcttaaata gcatggcgaa ttcgacaaca
    19441 gaattgtcaa gtaagcttgc cccagcgact acttgaccaa cagattggag tgtaccaccc
    19501 aaaagtgcac ttttttcaat gagatttcca ccaaacaact gtaatccaag aaaagggaga
    19561 gtcaacatca tcacagtacc cagtagattg actagtgtga tgatctgtcc tttttcttcc
    19621 tctttcgcat ggatggaagg ggcgattgaa gcaatagcag aagaaccaca aacagcgttt
    19681 cctccagcca tcatcaaaga agaagtatcg ctgaattgca gttttttacc aatcaagtaa
    19741 gcaccgatga tagtgactga catttgcagt aacacaaaaa caattccttt tacaccaatt
    19801 tgagcaatcg tttgagctgt gacagttgca cctagtaaaa aaacagagaa ttctaatagt
    19861 ttgctttcag caaattttgt gccgattgct agaacaggct gtttaaaaaa agtatttcct
    19921 aataaaatac ccaataaaat agcaatagtc gctccaccta actgtggcag ataaatagca
    19981 agaagcctgc tgaaggcagc gatggcaaaa gaaagcagta aaccgggtaa gatttcgcgg
    20041 accgctgaat cctttacaga ttttattgga tgtgacaatg atttcatttg atcggttcct
    20101 ttcgtcaata aatactaaaa gcatactcga acatagtagc actaaaccag tattttgaca
    20161 aaagaacaag aggtcgtgac aaaagtcttg tacgacctct tgtttcgaat a
//
LOCUS       DACDXE010000057        20030 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.057, whole genome shotgun sequence.
ACCESSION   DACDXE010000057 DACDXE010000000
VERSION     DACDXE010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.057"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(282..434)
                     /locus_tag="IWM23_002010"
     CDS             complement(282..434)
                     /locus_tag="IWM23_002010"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884623.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(625..1503)
                     /locus_tag="IWM23_002011"
     CDS             complement(625..1503)
                     /locus_tag="IWM23_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9884624.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(1542..2660)
                     /locus_tag="IWM23_002012"
     CDS             complement(1542..2660)
                     /locus_tag="IWM23_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAP9884625.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(2657..4756)
                     /locus_tag="IWM23_002013"
     CDS             complement(2657..4756)
                     /locus_tag="IWM23_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAP9884626.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(4807..5628)
                     /locus_tag="IWM23_002014"
     CDS             complement(4807..5628)
                     /locus_tag="IWM23_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9884627.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(5625..6434)
                     /locus_tag="IWM23_002015"
     CDS             complement(5625..6434)
                     /locus_tag="IWM23_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9884628.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(6455..6949)
                     /locus_tag="IWM23_002016"
     CDS             complement(6455..6949)
                     /locus_tag="IWM23_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9884629.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(6961..7449)
                     /locus_tag="IWM23_002017"
     CDS             complement(6961..7449)
                     /locus_tag="IWM23_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9884630.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(7526..10222)
                     /locus_tag="IWM23_002018"
     CDS             complement(7526..10222)
                     /locus_tag="IWM23_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9884631.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(10650..11723)
                     /locus_tag="IWM23_002019"
     CDS             complement(10650..11723)
                     /locus_tag="IWM23_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9884632.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(11720..12547)
                     /locus_tag="IWM23_002020"
     CDS             complement(11720..12547)
                     /locus_tag="IWM23_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884633.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(12548..13354)
                     /locus_tag="IWM23_002021"
     CDS             complement(12548..13354)
                     /locus_tag="IWM23_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884634.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(13366..14451)
                     /locus_tag="IWM23_002022"
     CDS             complement(13366..14451)
                     /locus_tag="IWM23_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884635.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(14468..15010)
                     /locus_tag="IWM23_002023"
     CDS             complement(14468..15010)
                     /locus_tag="IWM23_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9884636.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            15268..16257
                     /locus_tag="IWM23_002024"
     CDS             15268..16257
                     /locus_tag="IWM23_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAP9884637.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            16254..16991
                     /locus_tag="IWM23_002025"
     CDS             16254..16991
                     /locus_tag="IWM23_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAP9884638.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNCLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEETQSKSEIAALITEKIRLEESK"
     gene            16988..17539
                     /gene="coaC"
                     /locus_tag="IWM23_002026"
     CDS             16988..17539
                     /gene="coaC"
                     /locus_tag="IWM23_002026"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAP9884639.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            17555..18187
                     /locus_tag="IWM23_002027"
     CDS             17555..18187
                     /locus_tag="IWM23_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9884640.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            18272..18919
                     /locus_tag="IWM23_002028"
     CDS             18272..18919
                     /locus_tag="IWM23_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9884641.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            18924..19907
                     /locus_tag="IWM23_002029"
     CDS             18924..19907
                     /locus_tag="IWM23_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAP9884642.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMKANKNIGKIILKITE"
BASE COUNT         6176 a         4271 c         3474 g         6109 t
ORIGIN      
        1 taaaataaaa aatttattgc aaaaaaaata gaagtgacaa aattatcaat catttcaagg
       61 taatataact acctgagttt attatcgatt aaaatgctaa ctacatttgc aagagaagcg
      121 agagttaaag gtaaatatag aatttctact tttttagcaa tagaaatagg aataaaatat
      181 ttaaaatgta tagaaacttt cttttttttc ttgctatctt tttataatca caactctcta
      241 taatagaatt acgatttatt ttatacggct gaaaaaatct atcaccaaaa aaattcaaac
      301 attaagccaa ggattatgaa gtcaacattt cgaaaaatga acatacattg gtaagcaact
      361 ataactatta ccacagacat aaagtaacac aatatattac tatttaagtg tacgccacca
      421 gtgaatatcc tcaaggtata cagcgcacaa caaaaagaaa aaaggaaacg cagaagcatt
      481 tcctttcagt gatttaatga aaacaacacg atttgactta gagccttttt atttgaattc
      541 attttcgctt tatccaactt catttgatca tttgattact cggttaaata cttttgacat
      601 gagtatcaat gaaacattca ttaattatgg ttgcttattt cttaccgctt ttctggccaa
      661 tgctaatccg ccgattattc cggcattatc acctaattcg ggcgtaataa tgtagctatc
      721 aattggtggt atttcaacat atccatttat aagctcaaca aatttatcct gaactttttt
      781 cttcatgtgt tcttgtttca ttacaccacc gccaaaaata attctatctg gcgaaaacat
      841 cagtgttgta ttgtatgcac attgggcaag gtaaaaggct tcaatctgcc aaaacgaatc
      901 gtcagccaat aaattctggc cttttacacc caaccgtttt tcaatcgctg gtcctgctgc
      961 catcccctct aaacaatctt ggtggaaggg acagtttcct gaaaaattgt cctttggatg
     1021 tcgcttgatc aacatatgcc ccatttccgg atgactgaac ccttccacga aagtcccatt
     1081 gaacaaggca ccacctccga tacctgttcc cacagtgaag tatgcaacac ctgctaaccc
     1141 tttgccagaa ccagcaacat attcaccata gcaagccgca ttgacatctg ttgtccattc
     1201 aatcggaata ttgaaatatt gctttagcgt tcctacaaaa ttgaaatttt gccaagcaag
     1261 ttttggtgtt gatgtgatat gaccgaaact cgttgatttg cattgaatat caattggacc
     1321 aaagcagccg accccgatgc ctgccaattg ttccttaaaa ggtttaaaaa aatcaatgac
     1381 ctgcttcatt gtttcaactg gagttgttgt gggaaaactg actctttcca aaatcgttaa
     1441 ttcctcagta ccgactgcac agacaaattt agtccctcca gcttcaattg ccccatatat
     1501 catcataggt tcctcccttc gaatatgtga aaaacacttc attaacgcgg caagcgttct
     1561 cgatcagtga aatgacgttt agtatttcct gaagcttctg ttgaatacca gtaagcgaca
     1621 ctagcaacat cgtcttgccg ctcaaataag cgaacatcat cgttgccgat ttgttgtaaa
     1681 gtgaccgata aatcttcggt aaaacagatg gggtcttgaa gatgccaacg ataaagacca
     1741 tgtcgtggca ttgcgtcgtc accaaaggca tgaacggcat tcaattttcc cgtttcaaaa
     1801 cgatctcttg tggcatctct atttgattgg taaggatacc ctaaaaaggc tgttgaatag
     1861 tttttcgcat gtggacgtcc gaactcatcg tactcatgaa aggcccacgc cccgccaaaa
     1921 taatcttctg aacctgttga ggttacggtc ggatactcac tatccccatc aatgtagaat
     1981 ttaaactcgc cttcgcccca ccaataacgt tctaggcagg taagcgcaag gtaagtaccc
     2041 acgtaatagc cttgaccctt taatcgatca attaacttat aatcctcctg taatttggta
     2101 atgcgttccc gatgccaatt tgcgtgaaaa tataggctat tttcttccaa ttcgtccacc
     2161 aaagcgtaat ttatcgtata aaaaaatgag gacatatcct tcggatgtaa attctcgatc
     2221 gtaattttag cggatgtttg gaaaggcatt tcgaaatagg aattaaagcc ccctgtagga
     2281 ttaaccacta tcggcaacga gttcacctcg caacgctctc caaacccatt acaaaagaaa
     2341 tcccctaaag gactttcaac agcagggcta tccgacccat cccaataaat tcttaagatt
     2401 aaatcacgta aaacaaaact gccaaattct gttcgatcac gaatcgtcat ccacatatga
     2461 cgaattattc ccgttccagt aatatccgca atcacagttt cttcaccaga agtcagcgga
     2521 attgcgccac gacctttgcg gcttggtcct agctcgcttg gcaacatcgc agctttacct
     2581 ttctcgcctg ttggattttc gggagtgatt gctcgactct ggattttttt aaattgggta
     2641 atatttttta gtatactcat ctttttctcc tttgaatgtg aatcgttttt cccttttcta
     2701 cgtgaagaat ttttttcgta tcacttattt tcaatggctt ttctgacaaa tttgaaatac
     2761 tcaactcgga ttgcgtacat tcaatcgaaa tgaaggcttg accgaacttc aaattcttcc
     2821 aagaaaacgt taaattatca acaggatcaa tttgcaatac tccttttttg aatttcaatc
     2881 cgaaatattg actgatcatt aggctgacaa aaagacccga tgcccagccg catttgccgc
     2941 agccaaagtc tcgcacgact tctccttgtt gagcctctaa gcgatacggc caccaccacc
     3001 acgaaccgtc taaatctgcc aaacaaatta gttcttctaa tcgggttacg aaagcttgag
     3061 ggttgtgaag ctcgctcatt aaaacagtga taaagccagg aaaagtggcg ccggattcta
     3121 atccccaagt gatgcctttg atttcctcgc tataggttgg attgaaagaa ctagcagaaa
     3181 aggccatggt gttctgatat aaatgatctt tattatctaa aaattgataa aaaggcatca
     3241 acgtagtgtc tgattcttct ccatcatgca tcaataaatt aatttttcca tccacaatca
     3301 catcacttaa aaagattaac ccttgctcaa gaatcggttt ttgatagtgc ttcactgaat
     3361 aggttgtttt ctcctcatca ccgattccct ccaaaaattg ctcaccaaaa ggaccatcga
     3421 tagtcatttg tttacaaatt gattccttta gtttacaggc aatgtttttg tatcgcttgc
     3481 taagactcag ttgttctagg gcttcaaaaa tttctgcaag tccaatacag gccttccaaa
     3541 gacaaagatt gctgccggta tgatactttc caagtgcaaa ggcgtctgat aaccaaacag
     3601 atggaaaaag cattggttcc cctgaccgac gttgagctaa tatgtcctca ataattcttg
     3661 aagcattcag tagaacctgc ggacaccgct caaagaattt tgtatcccca gtatgttcaa
     3721 aataaagact agccagtaac gcactggata acgaattact cagagaatga ttgatacctc
     3781 ctgtaaattt tgaaccagga aacttaacgc catattgatc aaaccataga accgtttttt
     3841 gacataactc cggttcaaac atggtgaatg gcaatgatgc gtaatacata tccttattcc
     3901 aaattcgatt agtagcagga tagctgcccc aattgctgcc gacgacctgt ccctgtcgat
     3961 tcataccgaa actagaaaag ctttgataaa gtgcacgacg atagagatgg acaactctat
     4021 cgtttggcat tgacagctgc ccaaatagct gagcaaaata gttcaatgtt tccttcagcc
     4081 atttttcgat atcagattcc tgaaaagtca ttacttcttc aaaagcattt gggtccacaa
     4141 aagcaatggc gaagcttgcc acttcctgtt gtgcgatcgt gtccttattt cctttttgag
     4201 aaatcaacac attttgctga tccgaatact tctcttgata cgaaggcaca tctttcactg
     4261 aaacttgaag gctttcgtgt gtttcattca caatgtaaac ctgttgaaca agcatactta
     4321 aacgcttatt agccagtatg ggagcaaagg atactacaat gaccttaaac gtcgaataat
     4381 gatgaatata tctaggcaaa cagtcttgaa tcacatcgac ttcaatacga tgatccgatt
     4441 ctgacaaatg gtagacaatc ccgtttactt caacctttaa ggataacgaa ttggtttgag
     4501 agagctgttt attgacccaa acacctggtt tttcctcagt tagtaaattt tctcgataaa
     4561 aggtcaacgt ttgaatcgtc ccgcaaacat caaggtttgc taatgtcttt tgattactta
     4621 catcgaaggt caatcccttt acatcaggaa tattccgata gtagctgtat tcgtttgaag
     4681 tagtcacatt tccgtcgttt aactcagcat taaaaaatgg gtgtttgggt gaataaatta
     4741 cttcattaat agtcatcttt tccctccttg ctaaaaaaac tgcagcctag tagctgcagc
     4801 ttcctactat gcgattccaa gtaatgacaa taaaattccc aagacgacca ttccaaaaat
     4861 aattttgatg acgccgactt ttttatctag taatttgaaa cagctcaatg ttaccaataa
     4921 cggtacaatt cctttaaaaa tactatcgag gatttcttgg acattcatta gttcttcgcc
     4981 cttcattgaa aagctcaaaa cggtattgaa tttgaccata ctgatcgtca ttgcacctac
     5041 catgatcaga cccaagatac tagcagcttt tgtcagaatt tcaattaacc cgttcgcata
     5101 cagcttttca atatatttag agcctaatga atagcctaaa acgccgccat agtatcgcat
     5161 caagtaatta ggtatattga acataactaa aaagataatt ggtgccaaaa tatttccggc
     5221 attggctaag ttgatcgcta gtcccgcggt aacgacacgc caaactcccc agaacaatga
     5281 atcgccaatt ccagccaatg gtcccatcaa actcgttttg atcgcgttga tactttccac
     5341 gtcaaaacca gctttttcag aattttcctt ctccatcgat gccgcaatcc ccatgacaaa
     5401 ggacgcgcaa ggaatggtcg tcgaaaaata ctgtgtatga cgttccagcg ccgcagctcg
     5461 atcttcttca cttttgtaga aatgattaat gaagggcatc atagaatagg cgaaaccacc
     5521 gccgcccatc gtcaccggat tcaccgaagc atacatagtc atcgagcgcc aaaacatctt
     5581 ccgaaccaat tttttctcat ctttagaaat tccatcagct aatttcattg aaagaaatcc
     5641 tcctcttctt ggtcaagcgt tgcatttaca gcagcatcgc tttgaactgc atgaacagat
     5701 atatttttta gttgtagatc tctttgggct ataacaattg caataatcgt tccaactgcg
     5761 gcaattgcaa tcaatggtaa ctcgagatac gccataacaa cggccccaaa gaaaaaccac
     5821 acagcaatat ttttactcca aagcatccgt aacagcatcg ccatcccaac tgccggcagc
     5881 agatttccag cgactgttaa tccgttcatg atgacctctg gaatattatc caagacgctt
     5941 ttgacaacat ctgctccaaa taaaacggca aagaatggaa taagagcata aagcgcatag
     6001 tggacaaacc acataaagaa gtgcagattg atgattcctt tttgatcacc agattttgca
     6061 aatttgaaaa atttagcagc catcggtcca catacaaaag aaaaaatcat attcattaac
     6121 tgtaatccta atactgctag tggaaagaca atgggaataa tcgcatccac gtttcctccc
     6181 attttgattg cgaacgctgt agccagccca gcagccaaag aaggctccgc cgtactagag
     6241 attccgatat tgacgacacc cataaagatg gcttctagtg acgccccgac aatcaacccc
     6301 gtctgcaaat cccccatcaa cgctcctgct aacggagcaa caacaatcgg acgactcagc
     6361 atggacatcc cgaacatttc atgtccagca actccaataa aaacggccaa agcgactaca
     6421 aatgcatcat acataatttt tctccttcct ttttctaaaa atcactcaaa atagagatag
     6481 ctgaacgttt ttccatcgcc ggtgtaggct gaaaaacggt atctggataa agttcaacta
     6541 attcttttaa gtcttgaatt tctgacgtcg ttaacatcac ttcctttgat aaggttttct
     6601 tatcttccga tggcggtgtc attttcccag cattccccac gttcattaat tcgatttccg
     6661 ttgggaaagc tttcgcaact tttaccgcat ctttgacatt agggactaaa accatcaagc
     6721 gcatgtcctt cgcccgcgga tcttttaaca aatgaatcgc gtcatcaact gttttaataa
     6781 tgactttgac attcgatggt gcagccatct tcaatgtcat cttttgaata tcgttggtag
     6841 ccgcctcatc gcttgctact actaacccgg ttaaatttag actctttgtc caagtcatcg
     6901 ctacttgtcc atgaattaat cgttcatcaa ttcgcactgc tttaatcatt tttcttcctc
     6961 ctaaaaaaaa tcttcctctt catttactac atgtacctca tcaagaatgg tcatctttaa
     7021 ttcctgttgg catttattta ccagttcctg aatatcctcc tttgttaatt ccttggttgc
     7081 caatactatt tcgagcagaa ttggcagatt aaagccagac aaaatagtta cagcaatctc
     7141 tttctccaaa aaggttttgg taatcttctg attgacactt ccgccaaata aatcggtaaa
     7201 aacaaaaatc tgatcctgct cgttcacctg gtttacaaat acattcaact cctgttcaaa
     7261 atcacactca tccacataag cattaatcac cgtaatctct tgaaccttcc ccgtcagtaa
     7321 ttgaatactg ctaagaaagc ctgaagctaa gtgaccgtga gtcgctatta gaattttctt
     7381 catatggtta attcctttct ttctgttcac ttttatataa gcaattcctg tgccaacttt
     7441 taaaaacatt ggtttaatca cgttaattca cattaaaaaa agtgtctaac ctaaaaaaaa
     7501 cagattagac acttttttag acactttatt tttgaatata cggtcctaat aattcataga
     7561 gcagcgaaag ctcataagca ttgactttaa aattatattt catctccatt gattgaaaaa
     7621 tacttctaga aacataataa aattcatctt catctggttt caaatcataa ttcggttctt
     7681 cagatttttc tgtccgagcc aagaataaac gttccatcat caatgccgta tgcatataga
     7741 gatttagttt tacacggcca tttaaagtga aatgatagta attttcatat ttagaaatga
     7801 ctgtttccac ttcactcatc acgatcgttg ggttcagaaa gttcaaacgg tccgcaattc
     7861 cttccaacga aaagaatttt actagctcct gaatcaagcg gttaaattct gccggcttca
     7921 tttcaggact caaaatatgc tccaaattgg ctgctccatc accgtctaag atgtcataaa
     7981 cattaatatt aggaattgaa aaggaacgtg gaagatccga agtcgtgatg accaattttg
     8041 tcttgtcaaa ataagtttca tcattttggt cgattaattc acgtagttct ctgtagtcct
     8101 tcgtgaaaac ttcgatggat tcatccgata aatgatgacg aaaaacatct ttcaatttgt
     8161 ccgaaatccc caaacccgac atacaggaaa tgataatgtt gctactttga gaaaagcctt
     8221 caaagtattg aacattgatt gaatacttgt ctgctgcatc ctccgcaatc tgtttaaatg
     8281 gcgcattcat cgtcatcttc aagccaatat caagtgcaat ggaagtcgtc aaattattca
     8341 gaagaagtaa ctcccctttt aaatcgttct tgatctgtgt gtaaatttga tttaaagagc
     8401 ccatatctac gatcagcact agactatctg tattcacttg ccgtttcacg aaattccttg
     8461 tcgattcgac aatctctgac acattactct ctactggcat atccaacgct tcaaaaacat
     8521 aattgccgca taattgattc actactgctt gaatgctgct agccgtacta tctccgtgag
     8581 caagcaacag gcccttcagc tcgatggatt catctacata atcagataaa gtcacgacaa
     8641 taagcagtct tagaaagttc tcgtcctttt ctgagagcgg tggtaaatgc tccaagaact
     8701 gattacataa gtaagtcgtc cgaggtagat ttgcagctaa tgtacgattt atcccggtta
     8761 agtctgtctt atagcttggg gaaatgacat aggatctgct aataatttct gcaacaggtc
     8821 cactcgtcgt caatccgtac gtttttccta aaacggatac ccagaccttt ctaaacatct
     8881 gaatcatcaa ttttctactc attgataaaa aagaatcctt atcaatgtga tgcaccaaat
     8941 gctgtacttc attctttatc gaagaaaaat tttcattgaa attaccttca tttatcagct
     9001 gagtaatttt ttccaaaatc gaatcataat caaacggttt gattcgatcc gtttttatct
     9061 tttgattttt ctttatcaat aaactagcaa tcggcaagct ttctacttcg gattccagca
     9121 tatttaactg aatcaagatc tcatttttcc caatcgttaa tggttcttct tgtgttcgac
     9181 tatacacatc cgcacaactg attttaatga tattcttcag cttaccaata ttcccactaa
     9241 agggatgaga taacattaaa gacaaggctt ctcttgaaac aataatatct ttttgtaaaa
     9301 catccgcttc attttgaaaa aataattgaa tcagctctaa tcgttctcga atggggcgat
     9361 cttcaaaagt tggcaacata acactaattt gaatacgccg atcaaaggtg tctaataaat
     9421 aattttcacc tttttccgtc gtggcgaaaa taaaacgaac cttagcagaa tgccaattta
     9481 cattttcacc gactggacga tagtttccag tatcaataaa tagaaagagc ttttcttgat
     9541 tttctttcga taggcgatgg acctcatcga gaaaaagata gccgttgttg gccgttgcca
     9601 ataaaccatc ggtatccttt tcagcccccg taaaggcccc cttcttgtat ccaaacagtg
     9661 ttgccgaaag cagttcggga ttgtccgcat aatcggcaca gtttaagata gcaaatggtg
     9721 catctttttc aataatttgc tcatggatag cgtattcaaa aatttttgtg gctaaaaaac
     9781 tttttccaac acccgtggct ccatttatta atacactgag tccgttaggc ggatatttga
     9841 cagcggctat acatttttga atgacctctc tcagacttcc gtcatgacca actacagaat
     9901 gaaaggcttc gtttatttgc tccgtattat ctacaaccgc atattcatct aaaggcttcc
     9961 acaaaacagg ctttcccggt aatttctcga cctttttatc ttccactaat ctcgtcaaat
    10021 aatggctggc attctgacga gagataccta gattcttcga aattttctcg gcagtgacag
    10081 aagacatatt ccctgttagt cgtatcagct catcaaaaac tcgatcttta gcgctctttt
    10141 tcattttttc ctccagttta atgtgtctaa aagcataata aactatcggc tgcaatatgt
    10201 ctattctata tttgtattcc aatttttact ttgaaacaca aatagtcgaa tatttgcctc
    10261 agatatctga caaaataatt tatctaccaa tttagttttt atctaatatg atttttatgt
    10321 tttttcatag ctacaaatat tgaactggat tcatatatta gttttattaa tagattacag
    10381 aagggttaag tatatttgca tagtttccaa ttactttatc cttctgtaac tttttcaatc
    10441 ttagatttac tagcccgtct cgggccctat tttacaatgg ttttgtccac ttttctctgg
    10501 tagaaaaacg tggtagaaaa aataaaataa cacaaaaaaa gcggcgtttc atccgctttt
    10561 ggtagaagat tggaaaactc agatagaaaa aggacgattt ttagctcttt agaacgttga
    10621 taagtcccca ttcttactga gttttgccat tatctcctat acatcttgaa ttctaagaat
    10681 aggtcattat agatgcctag gtatttcgat cctagatctt tgtagacttc aagatgggag
    10741 atcgtgtcat cagatggata gaattgttcg tccgacgaaa tgtcttttgg caataacgct
    10801 tttgcttctt catttggtgt agaataacca atatactctg cattttgtgc cgcattttcc
    10861 ggttctagca taaagttgat gaaatcatag gcaccttctt tatttttcac ggtttttggc
    10921 ataacgatat tatcaaacca aagattggat ccttctggtg ggatcacata atgtaaatgt
    10981 tcattttccc acatcatatc ggcagcttcg ccagaaaatg tcactgccac ggaggcttct
    11041 tcattgatca tatacatttt gatctcatct gccacgattg ctttgacatt tggtgttaaa
    11101 tgattcaatt tatttgctgc ttctgtcagc tgtgccatat tcttactatt caaagaatag
    11161 cctaaactat tcaaagaaag ccccataact tctcgtgcac cgtcaatcag catcaaactg
    11221 tcttttaatt ccggcttcca caaatcatcc caatgcttaa tttgatttgc atcaaagaat
    11281 ttatcgttat atacgatccc taaagttccc caaaaatatg gaatcgaata ctgattatct
    11341 ggatcaaaat ccaagtctaa aaatcgtgga tcaatattat ttaatccttt gatcttcgaa
    11401 tgatctaatg gaagtactag tttttctttg atcatttttt ggatcatata ttcacttggt
    11461 atagcgatgt cgtagtttgt cccaccttgc tggatttttg tgaacatcgc ttcattagaa
    11521 tcaaatgttt cataattcac tttgtatcca tattcttttt caaatttcgt gatcaaacta
    11581 ggatcgatat aatctcccca gttatagatg gtaagtacct ttgctccact catgccactt
    11641 gttttttcaa gttgacgaac accaagcagc agcaccgcaa taatgatgag aatacctaaa
    11701 aacagagatt gtaatttttt catcgcagcc gtgccacccc ctcgcgtact cttctgtttt
    11761 tcttcagccg ttttggacgg ttttctttac taataaagta gtatccaatg accaatatca
    11821 tggagaacaa gaaaactaac gcacttagcg cattgatttc taaactgatt ccttgtcttg
    11881 cacgagaata gatctctact gagagcgtcg taaagccatt tcctgtgacg aaaaatgtaa
    11941 ccgcaaaatc atcaagagaa taggtaaatg ccataaaata tccagctatg attcctggag
    12001 aaagataagg caaaatgata tttttgacta cttggaaatt attagcacca aggtctctag
    12061 ccgcatcgac cattgaatcg ttcatttcct gcaattttgg caagaccatc aagacgacga
    12121 ttggaatgct gaaagcaata tgggaaagca atacactctg aaaacctaat ccgaatccta
    12181 gtagtgtaaa gaaaatcaag aaactagcac cgataataac atcaggagag actaataaga
    12241 tgttattcat gctcaacaac gctgtccgtg cacgtcgttt tttcgtgtaa tagattccca
    12301 tagccccaaa tgtcccgatg atcgtggcga tcaatgcaga taagaaagcc agcatgaacg
    12361 tattcaatac gataatgatc aaacgtgtat cttcaaaaac agcttcataa ttttcccaag
    12421 taaatcctgt aaagctgttc atcgttcctc cttcattgaa ggaataaaaa atcagataga
    12481 agatcggtgc atataagaat aaaaagacga tccctaaata aagatacgac catttaaatt
    12541 ttttcattta tgagcgacct ccttttttct tctcgcctgt caggagcatc acgatgaaca
    12601 tagcaatgat taaaatcaca ccgattgtag aacccatccc ccagttttgt gtaaccaaga
    12661 aatgctgttc gatagccgtt cctaaagtga tcacccgatt tccgccaatc agacgagtca
    12721 gcatgaataa agaaagagaa ggaataaata ctgcttggac accacttttc acaccattta
    12781 atgataacgg aaaaatcact cgtcggaagg tctcaaagct attcgcgcct aaatctctac
    12841 tagcactgat caaagaagga ttcatctctt ccaatgcatt gaaaattggc ataatcataa
    12901 atggaatctc gatatacgtt gccacgaaca gaaaactgaa gtctgtgaat aatatctgtt
    12961 tcggcccgat cccgataaaa tcaaagaaat gattcacgct gccgtgcaca ctgaaaatcc
    13021 caataaatgc gtaagcttta agcagcagat tcacccatgt aggcaaaata atcagcatca
    13081 gccataattg cttgtgcttc agttttgtta aaaaataagc agtcgggtaa ctgatgatca
    13141 gcgtgaaaaa cgtgatcaaa aatgcatacc atacagaatt gatcgtcata gaaagcgatg
    13201 tccccgacgt aaaataagat agataattat tcagcgtgaa ttgcccattc atatcaaaaa
    13261 aagattgata aatgatcatt aatacaggcg cgatcacgaa taaaccgagc cacaaaatat
    13321 aagggacgga atagatcctg cgcattgttt tcattcttgt cccccctatt cttcgtagct
    13381 gtcaagacgt gcatcgaatt cttcttcaga ctcgttgaaa cgcatgacat ggatatcttc
    13441 tggctcaaag gccaatccta ctttgcttcc ttctttagct tttttcgttg aatggaccat
    13501 ccattcattt tgctgttcat cataacagat gatttcataa tggacaccac gaaacagctg
    13561 agtatcgacc gttacgacta gttttccttt atctggtgtc gtgatcgtca aatcctctgg
    13621 gcgtaaaaca acttcaactg gttcatttag acgcatcccg ccatcgacac attcaaattg
    13681 cttaccaaca aactcaacta gattatcttc gatcattaca ccgtccacga tattgctctc
    13741 cccaacaaaa tcagcaacga aatgattgat tggctcatca taaatatcta cgggtgtacc
    13801 actttggacg attttccctt tattcatgac aaaaatctca tcactcattg ccaacgcttc
    13861 ttcttggtca tgagtaacaa aaataaacgt gattcctaat cgttgttgca gatcccgcag
    13921 ttcatactgc atatctgtac gtagtttcaa atcaagcgcc gataacggtt catctaatag
    13981 caatactttc ggttcattta caattgctcg tgcaatcgct acacgctgtc tttgaccacc
    14041 agacatttca ctaatctccc gtttttcgta tccaggaagc tggaccatac gcaaagcatc
    14101 ttttaccttc ttttcgatat ctgatttgga cattttcttg atcttcaatc caaaagcaac
    14161 gttatcaaaa acattcatat gaggaaaaag tgcataatct tggaagactg tatttacttg
    14221 tcgcttatta gctggcaaat cattcaaccg tttcccttca aaatagatgt ccccttctgt
    14281 agcttcggtg aatccagcta taatacgtag gatcgttgtt ttaccacagc cggaaggacc
    14341 aagcagtgta taaaattttc cttgttcgat ctcaaaactt acttttttta atactgtttc
    14401 atcatcatag cgtttcacta cttgatcaaa tgaaatgatt ggtttcccca cgtttttacc
    14461 ccctatatta caaatatgat tgcgttgcaa caatcaatac ttttgttcgt tttttgagat
    14521 gattccttaa ttgatgttgt tttgtcgctt gataatagat cgactgtcct gcttttgcca
    14581 catagaccgt ctctcctaag cttaactcta attccccttc taatacaaaa ataaacgttt
    14641 cagataatga aggttcaaac gtcttatatt ctcctgcacc ttcaaaagtc agcaagacag
    14701 gctccatttc tttttcattc gaatcagcca cgagccattc caattcatac ccgttttctt
    14761 catcgaaata aaccgtatga tcttcttttg aataaaccac ttgcagattc gctgcttctt
    14821 gatgaaaaaa ttcttctgga gtcacaccta agacttcaag aatagaaaag aatgtttcca
    14881 ttgatggtga acttaaatct cgttccaact gggaaatata cccttttgac aaatctgtgc
    14941 gttcgcctag ttcttcttgt gtcaaattct tttggatgcg caaatttctt aacttctcac
    15001 caatttccat aatttcctcc gaatcgaagt tttctaatag taaacttcct gtttactacc
    15061 actcgtatta gtatacgaaa tttattgaaa aaggcaaccc tttgtaaaat tttttttgct
    15121 gaatatttct ttatcttttg gaataaattc tagttgtttg attctttatt tttcacctaa
    15181 tttttgtcta aatttacgga agaacaagaa taaaagagta ggctttatgg tatgataaaa
    15241 gaaaatttta aaaggagtgt ctcacgaatg aacgcttttc gatcttttca ggtaataaag
    15301 gaacctgttt tccatacagc aatcagtgat ctgccattta ctgagttggc tgaaaatgaa
    15361 gtcttggtaa aaattgccta ttctgatgtc aattataaag atgcattggc aatgagcgag
    15421 tctggtcagg tgatacgaac atatccgatg actccaggga ttgatttatc cggaactgtc
    15481 gtccaaagta acaatccacg tttttcaaaa gaagatcttg tacttgctac tggttttggg
    15541 cttggcgtca cccatccagg cggttatagc caatatcaaa aagtaccagg agactggtta
    15601 gttcctctac caaaaaatat gacgctgcgt caagcgatga tccttggtac agcagggttt
    15661 actgctatgt tgtgtgtgaa cgctttaatc cgtcaaggta tgacctccga taaaaaagtt
    15721 gtcgttactg gtgcttctgg cggtgtaggc agtacagcga tagccctgtt acataaacta
    15781 ggattccagt caatcattgc cttttccaga aaagaagagt ctgtcacatg gctaaaaagt
    15841 ctaggtgctt tgcaagtcgt acgacctgat gaatttctgc cagagacaac aaaagcttta
    15901 ggaaaacagc aaatcgacta tgtcatcgac acagtaggtg gcgagcagct aagcagtctt
    15961 cttccattga tctcttataa cggtgcagta gctttatgtg gcaatgctgg gggaatcaaa
    16021 ctaaacgcga ctgtcttgcc ttttattctg cgaaacatcc agttgattgg aattgattct
    16081 gtcaatgtac caatagatca acgtctttct ttatggcaac agatggcgga tttgcagatt
    16141 gcagatgaac tggtcgtcca agagattact ctggatcaat tgcctgaaac agcatctaaa
    16201 ctacttgctg gaacacacca aggaagaaca ctagtaaatg taggtgacca taaatgaaga
    16261 ttctaatcac agctggagga acgactgaaa aaatcgatca agtacgtgct attacaaatc
    16321 attcgacagg acgattaggg caagcattag ctgattattt agctgccaat ccggatacca
    16381 caatcgatta cgtgactacg aaacaagcac tgaaaccgaa aagacactcc aatataacga
    16441 tttatacgat cgaatctgca ctggacttat ttttacaatt agaagaacta acgaaaaaag
    16501 aacactacga tgcgattatc cacagtatgg cagtcagtga ttttactcct gcttttagtt
    16561 tttcagaaga acagctggca aaaaacctac ccacttcttc aacgcaagaa gaactggaca
    16621 attggtttgc ggaaaatgaa cagacagata ccactgtttc aaagatctct tctaacaccg
    16681 agcatcttgt gctcgttttg aagaaaacac caaaaatcat ttcttatcta cgtgaatggc
    16741 agccaaaagc aaaaatcatt ggtttcaaac tattagtaga tgtaccaaag gaatctcttc
    16801 tggcagtcgc aaaaaattgt ctgataaata acaaaactga ttttattttc gcaaatgatt
    16861 tgacggaaat ccatggcgag acacaccacg gctatctctt atctaaagat ggaacagtgg
    16921 aagaaactca aagtaaatct gagattgctg cattgattac tgagaaaatt agactggagg
    16981 aatcaaaatg aaaaagatct tactaggcgt tactggaagt atttctgctt acaaaagcgc
    17041 tgatcttaca agtcaattcg tcaaattagg tgcccaggta gatgtgatta tgtctaaaag
    17101 cagcacacat tttatcacgc cattaacctt gcaatctctg tcgaagcgcg cagtccatac
    17161 agacgtcatg caagaatcaa atccgcaggt aatcaatcat atcgaactag caaaacaagc
    17221 tgacttgttt ctcatcgcgc ctgctacagc aaacattatc ggcaagctag caaacggcat
    17281 tgcagacgat cttctttcaa cagtcgcaat ggcgcttcca gtgggaacac caaaattgat
    17341 tgcccctgcc atgaacacga atatgtatca aaacccgatc actcagcgta atctggaaac
    17401 attgagatct gtcggctatc aggaaattga accaagagaa tctcttttag cttgtggaga
    17461 ttttggaaaa ggtgccttag ctgatattca gacgatcatc gaacaggtca tgctgactct
    17521 taatgaaaga aatgcttaga aaaaaggagt acttatgaaa aatacaaaaa actttacatt
    17581 aactgcgatg tttttagcta ttttgatttt attagctgtt acgcctttag gttttatccc
    17641 tatcggtcca atcaatgcaa caaccatgca tattcctgta atcattgctt cgatcgtttt
    17701 aggaccaaga ctaggtgctt ttcttggcgg tacatttgga ctgatcagca tgatccgtag
    17761 cacagttatc caaacacctt tgtcttttgt cttttctccg tttatcccag tcatcggaac
    17821 agaacatgga agctggaaag ccttgctgat cgcgtttatt cctcgcatct tgattggggt
    17881 ggttccttat ttcgtttata aaggtctgcg caaactaatg aaacaaaaag cagattccgt
    17941 ttctttattc ttagctggac tttccggttc attgaccaat acgattttag tgatgaatat
    18001 gatctacttt ttattccagc aagattatgc caaagtcatc ggaacaaatt taaatgctgt
    18061 ttattcagct attctagcgg ttatttttac aagtggtata cctgaaggaa tcgtcgcagg
    18121 tcttgcgact gcagcagtat gcaatgtctt gttaagatac ttcaagcacc cttctcttac
    18181 taactgatag tcatcgtagc cgatctctga tttttttctc ttttggtgtt actattgaat
    18241 ggaaagattt gacgtcgaaa ggatgatcca aatgaaaatt tttgttgtag gtgcaaacgg
    18301 acaaattggc cgacacctga taaaagattt agctccaagt tcccatgaaa tttttgcagg
    18361 ggtgagagat gttgcttccc aaacattagt aaaaaaagag aatgtttcgt atgtctcttt
    18421 tgatttaact tggtccgtgg aaaaaatgac cgaagcattc aaaggcatcg atgtattgat
    18481 ttttgctgcc ggctctcaag gaaagaatct tctgcaagta gacttggatg gcgcaatcaa
    18541 aacagtgatc gcagctgaaa atgcgcatgt ctctcgctat ctgatggtca gtgctgttta
    18601 tgcagatgaa ccagcaaaat ggcctgagtc catgacagat tattatatta ctaaacatta
    18661 tgcagatgaa tggctgaaac gaactaactt agattttgtc attcttcaac cagtcacatt
    18721 gactaacgat gaagaagtta cttccattca attaactaag cctaatgaga aggcatcaaa
    18781 aacgatcact cgctctacag ttgctgctgt acttgccgct ctagtggaag aaacggatat
    18841 ttctcgtacg actctagtac tttctgaagg tagcaaagag ttgaatacag cattccaaga
    18901 atgggcaaag gaggaataga tccatgcgtg ccatttcaat caagcatcct ggttctgtag
    18961 atgtattaga acaaattgaa gtgcctgttc ctaaaccaaa acaaggcgaa ttactgatca
    19021 aagtccatac agcagctgtc aatcgaacag atattatgaa aagagaatca gtctctttga
    19081 aacctcccta ccctattttg ggagtcgaag tagctggaga ggtagttgag aataaaagtg
    19141 atatctccac ttttactcct gggacaaaag tatacggatt agtcaatcta ggtggttatg
    19201 ccgaatatgc tgtgatgcct gccgatcgtg caatccttct ccctgatacg ctagactatg
    19261 tttctgctgc tggaatctca gaagtttttt tgactgctta tcaaacactt tactggcttg
    19321 gaaaattaca agaaggcgaa actgtcttga tccatgcagg agcaagtggt gtgggaacag
    19381 cagcgatcca attagctaga cagctttctc acgcgaaaat catcgttact gcaggatcag
    19441 aagaaaaact cgccttttgt aaagaacttg gtgcagatga attgatcaac tataaaaaac
    19501 aagactttca agaagaggta cgaaaaatca ctgaaggtcg gggagtcgat gttattttgg
    19561 atttcatcgg cgcttcttac tgggaaaaga atttagcttc tatagccgtt gatggacgtt
    19621 gggtcttgat tggtatgcta ggtgggacca tcgtgcctga gatagacctt ggtgtattga
    19681 ttgggaaacg tgtccaactg cttggtacat tattgacacc tagaagtgat cagtataaag
    19741 cagagctcgc acaaactttt atgcaaaaag ctgggccttt tctagaaaat ggcaagattc
    19801 ggccaatcat tgaccgtaca ttccaattca gcgcagtcaa acaagcgcat gaatatatga
    19861 aagcaaacaa aaacatcgga aaaatcattt tgaagataac tgaataaaat catgctgatc
    19921 aaaaaagagg ctgggacaga agcgtttaac tccaagaaat aagaagaaat tcacgaaaat
    19981 tgcttttcaa atttttgtga atttcagctt atttccgaag gaatttcttc
//
LOCUS       DACDXE010000058        19578 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.058, whole genome shotgun sequence.
ACCESSION   DACDXE010000058 DACDXE010000000
VERSION     DACDXE010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.058"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..504
                     /locus_tag="IWM23_002030"
     CDS             <1..504
                     /locus_tag="IWM23_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAP9884643.1"
                     /translation="FQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVSTPADF
                     RMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQLKNNE
                     VIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSRIDKVR
                     YMFNKIT"
     gene            602..790
                     /locus_tag="IWM23_002031"
     CDS             602..790
                     /locus_tag="IWM23_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9884644.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(848..1201)
                     /locus_tag="IWM23_002032"
     CDS             complement(848..1201)
                     /locus_tag="IWM23_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884645.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1732..2154
                     /locus_tag="IWM23_002033"
     CDS             1732..2154
                     /locus_tag="IWM23_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9884646.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2151..2381
                     /locus_tag="IWM23_002034"
     CDS             2151..2381
                     /locus_tag="IWM23_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9884647.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2879..3079
                     /locus_tag="IWM23_002035"
     CDS             2879..3079
                     /locus_tag="IWM23_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9884648.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3106..4299
                     /locus_tag="IWM23_002036"
     CDS             3106..4299
                     /locus_tag="IWM23_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884649.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="IWM23_002037"
     CDS             complement(4367..5932)
                     /gene="guaA"
                     /locus_tag="IWM23_002037"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9884650.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5991..6914)
                     /locus_tag="IWM23_002038"
     CDS             complement(5991..6914)
                     /locus_tag="IWM23_002038"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAP9884651.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7110..7715
                     /locus_tag="IWM23_002039"
     CDS             7110..7715
                     /locus_tag="IWM23_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9884652.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            8023..9039
                     /locus_tag="IWM23_002040"
     CDS             8023..9039
                     /locus_tag="IWM23_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884653.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9180..10481
                     /locus_tag="IWM23_002041"
     CDS             9180..10481
                     /locus_tag="IWM23_002041"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAP9884654.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10527..11189
                     /gene="deoC"
                     /locus_tag="IWM23_002042"
     CDS             10527..11189
                     /gene="deoC"
                     /locus_tag="IWM23_002042"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAP9884655.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11210..11608
                     /locus_tag="IWM23_002043"
     CDS             11210..11608
                     /locus_tag="IWM23_002043"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAP9884656.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11674..12756
                     /locus_tag="IWM23_002044"
     CDS             11674..12756
                     /locus_tag="IWM23_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9884657.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12968..14530
                     /locus_tag="IWM23_002045"
     CDS             12968..14530
                     /locus_tag="IWM23_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884658.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14523..15656
                     /locus_tag="IWM23_002046"
     CDS             14523..15656
                     /locus_tag="IWM23_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884659.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15656..16612
                     /locus_tag="IWM23_002047"
     CDS             15656..16612
                     /locus_tag="IWM23_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884660.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16711..17877
                     /gene="deoB"
                     /locus_tag="IWM23_002048"
     CDS             16711..17877
                     /gene="deoB"
                     /locus_tag="IWM23_002048"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAP9884661.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17901..18719
                     /locus_tag="IWM23_002049"
     CDS             17901..18719
                     /locus_tag="IWM23_002049"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9884662.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18736..19443
                     /gene="deoD"
                     /locus_tag="IWM23_002050"
     CDS             18736..19443
                     /gene="deoD"
                     /locus_tag="IWM23_002050"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAP9884663.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
BASE COUNT         6256 a         3523 c         4217 g         5582 t
ORIGIN      
        1 tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt atatggttgg
       61 aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc tgttagtact
      121 ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag aaaagctgga
      181 acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga atatctttca
      241 cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca actgaaaaat
      301 aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta tcgcaatgat
      361 ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg atatcaggtt
      421 accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga taaagtaaga
      481 tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat ggtttcttat
      541 taaataagat gaaatattct ttaatataga tttgaattaa agtggaaagg aggagattgt
      601 tattataaac tacaagtgga tattgtgtcc tatttgtgga aataaaacaa gactacgaat
      661 acgagtggat actatactta aaaatttccc tttatactgc cccaaatgta agaacgaaac
      721 tttaattaat gttcaaaaaa tgaatataat aacaatcaaa gagccagacg ccaagacgca
      781 gagccgataa tttgagaaat gaaactctca tcttatcggc tctttttgtt tatctgaatt
      841 ttactgacta gccttcaata tttcttgctt cattgatacc ttttgctagt gcttccatta
      901 aggatagttc tttgtctgta aagctatcca tgtatttttc tacctgtaac cttcgagtgc
      961 ttttaatcaa attatctgta ggtaagaaaa attcatcaat agatacatgg agtaatgata
     1021 caagatcata cagcacttgt gtacttgggt gttgtccttt gttttctata tttgttaaat
     1081 aacgtgggtc aatttcaatc attgttccca cttgttcacg agtgagaccc cgtttcattc
     1141 gagcctcttt aatcgctaaa ccaacggctc taaaatcgta tttatcttct tttttacgca
     1201 tactaaatca cctctataca ttttactgtt cctatataac cataaacagg tatagaaaaa
     1261 catgttatat agtttatgag ttcctataga acactgtgga atttattaat gcgatgaatc
     1321 caatttcaaa tagaaaggtc gctgatttta tttacctata caactttata aaatttagtc
     1381 atactcctag aaagtagata aaattggcga cttaatggag ggaaaagtcg ccatgagaaa
     1441 gataatctgt catctagttc tcttaattaa gttcttatca aaaaaactta tccacctgta
     1501 ctggatagat atgaccatca atgtggtttt gcagattaca tagatagaaa aatcaaagca
     1561 tatgaacaga gaaaacaatc tgtttatgtg cttttttggt cacttaataa atatctttta
     1621 ggatttctct tcatttatgc aacaaatccc ccgttcgtgt cggggtttag tatgaaggga
     1681 gataaagaag cacatgccac tccctttcac gacagaaagg gggtgaaaag catgaaacca
     1741 tcttcttttc aagaaaccat agaaaatcag tttgactata tctgcaaaca agttattgaa
     1801 gatgaacgaa aagattactt caaacatcta gacagactat cagcaaaaga agtctcgttt
     1861 tctgatatag ataactacct tgtcaactct ttttctactg ttgatacgta cgtcactgat
     1921 ttccaactat ttaaattatt tggattaaga atcaatgttg aaagtgattt attaagtgaa
     1981 gccctgcgtc atctttcaga gaaaaaacgg aacattattt tacttcatta ctttatggat
     2041 atgagtgatg tggagattgc ggagttacta aatttgaatc gttcgactgt ttatcgtcat
     2101 cgaataagtg gactagccat gattaaagaa ttcatgaagg agtgtaaaga atgaaaacag
     2161 cttatccaag ggttcccttc cccctcattg taaaggcgac agatggcgac gtggaagcaa
     2221 ttaatcagat tgttaaacat tatcgagggt atacctcaaa acgttcatta cgtcgcatga
     2281 cagacgaata tggaaattct catatggtta ttgatgaaac cctacgtggc agaatggaaa
     2341 cgaaactcat ctctaagatt ttatcgttcg aaattaaata gtcccatttt ttcgtggaag
     2401 cgtgtcgaat cgtatccacg cttcccgatt tgggaggttt gttattccat caaagcatat
     2461 tgtgtgttca gtaggttttg ataggctaac aagccaaacg ttctttgaca accaaataaa
     2521 ggcaatcgaa tacgtttcgg taagaaaaga gctgatgaac taacacgcca tgacctacaa
     2581 aattagtgag cgattcatgt tagtaattcg aaagtaatct ttagcaggat tgctgccatg
     2641 actttcttat cgtgataatg atactcccgt atagccatag tccgagcgtt agaagcgtca
     2701 caggcaatgg gtacggctac atgagaacca tgcagaagtg aaattcctgt gagctttgct
     2761 agagctgttc gattgctaag atattcttct cttgtcagtg tattattata aaactatttg
     2821 atataattat aggtgaatat tggagttttc ctagcctata attgggaaag gagtaataat
     2881 gataacggtc acaaaaaagg atttagttga gctaggatat ggaacttctt tttcagcaga
     2941 catcataaaa aaagcaaaag aattgatgat tacaaaaggg catatctact atcaatctcg
     3001 aaagctagat agagtccctc gtgaagctgt tgaagaaatt ctaggaattc ggttttcaaa
     3061 tgaaaacagg tctgagtagt ttttgcacag taaggagtaa aaactatggt taaagaccca
     3121 ataaaaaaag caaagaatgg aacctattat tttcgtgcaa atctaggtta tgattctaaa
     3181 gggaaaaaaa ttcaaaaata tcgtagtgga ttcactacta aaaaagaagc aagagaagaa
     3241 tattcgaaac tgctattgat gaaaccagaa gaattaagtg agaataagga taaaatgacg
     3301 tttaaacatt atatctttga aattttcctt ccatggtata agacacaggt aaagctcaga
     3361 acatatgaaa atcgcttacc aactattaag aagcatttct cttattttga taaaatggct
     3421 gtttccgata ttgagcctat tgatgtgcaa aattggcaac taaaattatc aaagaagtgt
     3481 aagtcttctt atgtaagagc tgttcaagga ctattttcag ttgctatgga tcgagcaatt
     3541 gtccttggga taaccaccac aaatccatct aagattattg gcaatgtgaa aaagcaaaag
     3601 tctaaaattg aattttggac aaaagaagaa tttgaaaaag taatctctct aatttacaaa
     3661 gaagattact atcaacactt tttatttatc tctctgtggt ttttatttat gactggaatg
     3721 cgaattggtg aagctactgc cattcaatgg gaagatattg attttgactc aggagtacta
     3781 accatcaaca aaacactcta ttacaagaat caatcgaatt atagatttgt tgaaccaaaa
     3841 acaaaggcga gcgttcgtca tattgtatta gataaatgca cattaactta tttatccgaa
     3901 tggaaggaag tacaacaaaa tctaattcag acagactttg taatgagcta taacggtatt
     3961 cctacccaaa aacatacact atcatatgct attgaacgct atgctaagct ggctggtatc
     4021 catcgaatca gaatccatgg gttacggcat tctcatgctt ccttgttaat cagtatggga
     4081 gaaaatccac ttatcattaa agatcgtcta ggacacgaag atattgagac gactttagga
     4141 acgtatggac atctttatcc taacagtaat ttcgaggtag ctaacaaatt aaatggagcc
     4201 atttccttta aagaagcaaa acaaaattat gatacttcac caaagaatca atttactgta
     4261 gactacttgc gaaacaaaga aatgaaaaaa gtgcaataaa agtgcagtaa aaagataaga
     4321 agagccagaa acccttggta taagaggatt tctagctctc ctacaactat tcccactcca
     4381 ctgttgctgg tggtttagaa gtgatgtcat acacgatacg gttgacgtgg gctacttcgt
     4441 ttacgatacg tacagagatt ttttgcaaga cttcccatgg gatacgggca aagtcagcgg
     4501 tcattccgtc gattgaagtt acagcacgga taccgactgt gtagtcgtaa gtacgtccgt
     4561 cacccataac accgacagag cggatccctg gaagaacagt gaaatattgc cagatatcac
     4621 ggtcaagtcc agcagctgcg atttcttcac gcaagatagc atcagattcg cgtacgattt
     4681 ctagcttgtc ttcagtgatt tcgccaagaa cacgaatacc aagaccagga ccagggaatg
     4741 gctgacgcca aacgattgaa tcaggcatgc ctaactgagt ccctaattca cggacttcgt
     4801 ctttgaataa tgtgtttaat ggttcgatca attcaaattg catatcttct ggcagtccac
     4861 ctacattatg gtgagatttg atcgtctgtg ctgtttctgt accagattcg ataacgtcag
     4921 tatacaacgt tccttgtgct aggaaagaga cgccttcttc accagcaagt tttgttgctt
     4981 cgtcatcaaa aacatatacg aattcattcc caatgatttt acgtttttgt tctgggtcag
     5041 atacaccttt caacttgtct aagaagcgat ctcttgcatc gactttgatg atgttcaagc
     5101 cgaattttcc gcctagactt tccattactt gttccgcttc gcctttgcgt agcagaccgt
     5161 ggtcaacaaa gatagatgtc aattggtcac cgatcgcttt ttgtaaaaga accccaacga
     5221 cactagaatc aacgccacct gaaagaccaa gcaagacttt tttgtcacca acttgttcac
     5281 ggattttttc tacttgcata tcgataaagt tatccattgt ccagttacct tcacagtgac
     5341 aaacatcaaa ggcaaagtgg cgcagcaatt cgattccgta ttcggaatga cgtacttctg
     5401 catggaattg gataccataa aaattacgtg ttgtgtcttg gatagaagca atcggacaat
     5461 ctttgcttgt tccgactgtt tcaaagcctt caggaacttt agttaccaag tcaccatggc
     5521 tcatccatac tgtttgttgt tttggtgttt ctttaaacaa gacagcttga tcgcttgtta
     5581 cttctaacaa ggctttccca tattcgcggt ttttagcagg ctctacggaa ccgccaagtt
     5641 tgtacgtgat caattgcata ccatagcaaa tccctaatac aggaatacct aaattgaaga
     5701 tctcaggatc gatcccaaat gcattctcat catatacact gtttggtccg ccagaaagaa
     5761 tgattccctt tggattcatc gcttttactt cttctgcagt aatgcggtgg ctcaataatt
     5821 cggaaaagac tccaagatca cgaatacgtc ttgtgatcaa ttggttatac tgactaccgt
     5881 agtctaatac gataatcttt tcaacgttcg tcatatcggc aacgtttgtc aaatttatta
     5941 cccctatccc tttaaatttt atctcaaaat agtcaaacta ttttaaaatg ctaatatttt
     6001 cgcatgaaaa tcgaatcgat gatatgattt tcagttttat ggagaattat atcggctctt
     6061 cctcgtgtag gcaagatata ttcctgtaaa tttttcaaat tcacattttt ccatacatct
     6121 tttgccattt taaaggcttc ttcacgatct ccgatagcaa aacgataata ataattctct
     6181 ggattttgga aagccgtatc cagtaacgca ccaaaacgat ccaaatacca tttttcgatc
     6241 agcacgggat cggcatcgac atagacagaa aaatcaaaaa agtcgctgac gtagatttgc
     6301 tgattcgcag gtaattgaag cgtattgatc ccctcaacga tcaagatatc aggctgctgg
     6361 atcaattcgt acttgtctgg aatgatatca tacacatcat gtgagtaaac aggtactttt
     6421 atctcttcct gaccagattt tacacggttc aagaaatcga tcagcatctc catatcataa
     6481 ctttcaggaa atcctttacg gtccatgatt ccacgctctt ttaatatacg atttggataa
     6541 agaaatccgt cagtcgtgat cagctgaacg tttcttcgtt tgaaggtacg agacaaaatc
     6601 gtctgcagca gtctggctgt tgtacttttt ccaacggcta cacttccggc gatcccgata
     6661 ataaaaggcg gtacttctac gtatttatga agaaataaac ctttactcaa gaacaaagat
     6721 tcaaattctt tcatataaag ataaatcaga tgagcaagag gtacatagat atctcgaaca
     6781 tcctgcatgg aaatgcggtc attcaaactt ttgatattat ctagttcatc ttgtgtcaaa
     6841 ggtgcttgcc cattacgata aaattctcgc cattcttctc ttgccacacg gtagtagttc
     6901 attggatcat tcatattagc ctcctgcgtt ctcccatcaa tggaactatt ttaacacaaa
     6961 ataaacgcaa accccaccac ttaagagaga ttgccgatct ttttgtataa tctttataat
     7021 agaaagaaac ggagtcttat tcttgacttg cgcacggata cataaaagga tagactgttg
     7081 tcatgaaaga aatgagaagg atggaagaca tgacaaatca ttactattct gaacgcccag
     7141 atacggcgca cgattttgaa caatgggctt ttgagttaaa aggcagaacc tttcaatttg
     7201 taacagattc tggtgtattt tcacgtgaaa cagtggatta tggttcacgt gtcctgattg
     7261 atgctttcga ttgggaagag ttacctgatg aaggaaagat cttagatgtg ggatgcgggt
     7321 atggaccgat tgggttagct ttggcatttg cgacacagcg gtttgttgaa atggtcgaca
     7381 ttaatgaacg agcggtaagt ctagcgcaag gaaacgcaaa gcgtaaccaa atcgaacatg
     7441 ttgatatcca tcaatcgaat atctatgaag cagttcatga agaaacctat gcagctatcg
     7501 taagcaatcc gccgatccgt gctggaaaaa aagttgtaca tgaaattttg acaggcgcat
     7561 atcctcggtt gaagaagggc ggtacactga caattgtgat ccagaagaaa caaggagcgc
     7621 cgagtgcgca aaagaaaatg gaagaaacat ttggtaatgc agaaatcgtc acaaaagata
     7681 aaggatatta tatcatcaaa agtgtgaaag aataactagc tgaaaaatag tggagcgacg
     7741 aaagagtgga actgggcgtg catgtacggg tacgtatcag ttcttttttt gttgagaagt
     7801 ttttaaaaac aaagaactat tttttcttca aaaagaaata gtaggaacag agtcgttgtt
     7861 ttttacgata aaataaataa gatacagaac ttgtatagaa ggctgccaag tcggtagata
     7921 aaggaggttt ttatcaaaaa acatactttc cttttttctc tattcttttc ttgggggttt
     7981 tggtacactg atagggaatt tagatagaag ggagccaata ggatgaagct gaccatcaga
     8041 gatatcgcag aaatggcagg cgtatcagta acaactgtct cacagatttt gaataacaaa
     8101 ggaagcagat ttagtgataa gacaagaaaa aaagtgttgt cgatcgtgaa tgaacatcat
     8161 tataagcccg attatttcgc ttcaaatgtg atcaatcgac attcaaagac catcggaatg
     8221 atcgtccctg atgtcactga tttcttcttc tccaaactga tcgaaggtgt agagagctat
     8281 ttgaatcctt tgggctatgt gatcatgtta tgcaattctc gtcatagcca agaaaatgag
     8341 atcaagtatt tacaagagct ttcccaccgc tcagtggacg gaatcttgct ggccacacca
     8401 aatattttac ctgagaaata tgcattagac agcggttttt atcaaaaaat gccaatcatt
     8461 ttaatcgatc gcggattgaa tcgtcgggat aacggacggc ttatcgtaaa ggaatatgag
     8521 ggcgcctatc aagctgtttc gctgtttatt gaaaatggcc atacgaagat cggcatgctg
     8581 aaggaaacca ctggatacta tcaattagaa gaacgtttca acggatatcg tcatgcgctg
     8641 aaagatcatg gcctgccttt taatgggaaa tttgttgaac atggagagtt gactgtccaa
     8701 ggtggatata ctgcatcaaa aaaattactc aaacataaag atatcacggc tattttttgc
     8761 gggaatgatg ccatggcgat cggatgctat caggcgatcg atgaattggg caaaaaaatc
     8821 ccagaagata tctcagtcat aggattcgat ggattaaagc tgtcagaata catgattccg
     8881 caattgacga cagtgcagca gccaagtttt gatattggat tttatgctgc acgtttcctg
     8941 atcgatacga tcgaatttcc tcaaagaaaa gttccgaaca aagtgttcga aacaaaatta
     9001 attattcgtg agagcgtaaa agtattgaca aagggctaac tcggcggtat attgcttatg
     9061 taagcattta caaaggctag ttagtgcttt ttttattttt agtaaaccag tttactaaag
     9121 tggtttacct ttcctaaaaa taatgtataa taccgaatga agacaaggag gatcaggcaa
     9181 tgcgaatggt ggatctgatt gaaaaaaaga gggatggaaa cgagctgtcg aaagaggaaa
     9241 tcgagtacat cgtcacgaac tatacgaatg gaaagattcc agattatcaa gtcagtgcat
     9301 tattgatggc aattttttat caagacatga caaatgaaga aatcacgaac ttaacattgg
     9361 cgattgctaa ttctggtgat gtcatcgatt tgagttctct tgaaggaata aaggtagata
     9421 agcattcgac aggtggcgtt ggtgacacga ccacattgat tttagccccc ctagtagcta
     9481 gtgtcggagt gacagtggct aaaatgtctg gacgcggtct aggttacaca ggtggtacac
     9541 tagacaagct agaagcaatt cccggatttc agatcgagct ttcggatgaa gaatttgtac
     9601 gcattgttaa tgaaagtaaa gtagcggtaa ttggtcaatc aggaaacttg gcaccggcgg
     9661 acaaaaaatt gtatgccttg cgtgacgtca cagcgaccgt tgattctctt ccgctgattg
     9721 ccagttcgat tatgagtaaa aaaatcgcgg caggagcaga tgcaatcgtt ttggatgtga
     9781 ccactggaga cggtgctttc atgaagaaca tcgaagatgc aagacgtctg gcaaagacca
     9841 tgacgagcat tgggaaactg gctaaccgag aaacagtggc ggttatctca gatatgtcag
     9901 aacctcttgg cgaagctatc ggaaatagtc tagaagttgt cgaggcaatc gaaacattac
     9961 aaggaaatgg tccagaagat ctagtagaaa tgtgctacgc cttaggaagc caaatggttg
    10021 ttcttgctgg caaagctaag acaaccgatg aagcaagaac gttgcttcaa gaagctctgg
    10081 aatcaggtaa agcgttagca aaattcaaag aaatgatcca aaatcaaggg ggagacccag
    10141 cgatcgtgga acatccagaa cgaattttga ctgcacgcta cacaatggag cttcctgcta
    10201 aacaaagcgg agttgtgtca aaaatcgtgg caaatgaatt aggaattgct gcaatgatgc
    10261 taggtgctgg gcgtaaaacc aaagaagaag atattgatca cgctgttgga ttgaaactgc
    10321 ataaaaaaat tggtgacact gtgacaaaag gtgagtcact attgacgatc tatagtaacg
    10381 acgaagaaat cacttctgtc attgaattat tatataaaaa tattgaaatt ggtgaatcgg
    10441 caatgaagcc aacgttgatt catgacatca tcacagaata aaggaaaaaa gcggacttta
    10501 ccgttttaaa gaaaagggga aaagaaatgg aactaaaccg aatgatcgac catactattt
    10561 tgaaagcaga cgcatctaaa gaagctgtga tgcaaattat cgaagaagca aaaaaatacc
    10621 atttttattc tgtctgcatc aatccagcat gggttgcgct agcgaaagaa caattacaag
    10681 gtgagccagt agccgtatgt acagtaatcg ggtttcctct aggagcaaac acacccgaga
    10741 caaaagcatt tgaaacaaca gatgccatca ataacggtgc agatgaagtc gatatggtca
    10801 tcaatatcgg tgctttgaaa tcaaaggatg atcaacaagt ccaaaaagat atcgaagcag
    10861 ttgttgaagc agcaaaagat cgtgcgcttg tcaaagttat tattgaaaca tcgttattag
    10921 atagagaaga aatcatccga gcttgcgaaa tagcaaaagc tgcaggcgct gattttgtca
    10981 aaacatcaac tggtttctca acaggcggag caaaggtaga ggatgtccgt ttgatgcgtg
    11041 aaactgtagg accagaaatg ggtgtcaaag cttccggcgg tatccataac gaagaagaag
    11101 caatggcaat gatcgaagct ggtgctacaa gaatcggaac aagcgcagga gtagcaatcg
    11161 tatctggttc aactggtgaa ggctattaat tttttcaaag gagaagctca tggcagaact
    11221 caaacaagaa tggatcaaca cagctattga agcgttagat aaagcctatg ttccttattc
    11281 ccattttccg gtaggcgctt gtctcgtaac ggaatcaggc aagatttatc aaggcatcaa
    11341 cattgagaat gcatcatttg gattaacgaa ttgtgccgag cgcacagctt ttttcaaagc
    11401 agtatctgaa ggagaaaaaa catttacgca tctagtcgta gcggggcaca cccccgatcc
    11461 tatctctcct tgtggtgcat gtcgacaagt aatggcagaa ttctgttccc ctgatatgcc
    11521 ggttacgcta gtaggagaca atggtgtgac gaaggcaacg accgttcgtg cactgctgcc
    11581 ttatgcattt actgaaaaag atctataact catgttcatt cagtcagctg actgttgaaa
    11641 aataaaacaa ttattttagg aggctttaca gaaatgaaaa aagcaaaatt atttggttta
    11701 ggtgctgtcg cactagcagc agggttattt ttaggtgcat gcggcaacaa cggctctact
    11761 gattcatccg gtggcaaaag ctcatcagac acaacaactg cagcattgat cacagacaca
    11821 ggtggtgtag acgaccgttc attcaaccag tcagcttggg aaggactaga aaaatggggg
    11881 aaagatcaag gactttcacg aggaaatgat ggcttccaat atttccaatc ttcaaatgaa
    11941 tcagattaca tcccaaatat tgaccaagca ttgaatgctg gattcaaaac gatttttggt
    12001 attggttata aactaaaacc agcgatccaa gaacaagcaa caaacaatac aggtacgaac
    12061 tttgttatta tcgatgatgt catcgatgga ttagataacg ttgtatctgc aactttcaaa
    12121 gacaacgaag cttcttatct tgcaggtgtt gcagcagctt atacgactga aacaaacgta
    12181 gtcggcttta tcggcggtgt aaaaggtgaa gtaatcgatc gttttgatgc tggattcaaa
    12241 gccggtgttg atgcaggtgc gaaagaatta ggaaaagaaa tcaaagtatt gaatcaatat
    12301 gctggtgact tctctgctcc agataaagga cgttcaattg ctcaaggtat gtatgcacaa
    12361 aacgcagata tcatcttcca tgcttcaggc ggtacaggta acggtgtatt ccaagaagca
    12421 aaatcattga acgaatcagg cgacaaaaaa gtttgggtta tcggtgttga ccgcgaccaa
    12481 tcagacgaag gcgaatacac attaaacggc gaaaagaaaa acttcacatt gacatctaca
    12541 ctgaaagcag ttggaacagt agtagaagat ttggcacaaa aatcagcgga cggtaaattc
    12601 cctggcggcg aacacactgt ttatggattg aaagaagacg gtgttggtct aacagaagga
    12661 caactttctg acgaagcgaa aaaagcagtt gatgaagcaa aagaaaaaat catttcaggt
    12721 gacgtaaaag ttccagaaac tcctgaagaa aactaaaacc atttctatag ccttgttaga
    12781 cacatgtttg acaaggctat ggtttgatta gaacaagaat tgttcattta tccaagctgc
    12841 cttgtgacag tttggataag ggaacaactc cctgaagtaa tgtttgtttc aaaagaagaa
    12901 cgctcactaa gcgtgaatct tagtcaatcg ttgccctaga tagaaatcgt taggatgtga
    12961 aaaaaaggtg gttcaagaaa atattgtcat agaaatgcag aatattacca aagaatttgg
    13021 aacattcaag gcaaatgaca acatcaattt acaagtaaag gcaggagaaa ttcatgcgtt
    13081 gcttggagaa aacggtgctg gcaaatctac attgatgaac gtgctttccg gattattaga
    13141 gccgacatca gggaaaattt tgatgcgtgg gaaagaagta aagatcacaa gtccgaccaa
    13201 agccaaccaa ttagggattg ggatggtcca tcagcacttt atgcttgttg atgcctttac
    13261 tgtaacagaa aacatcgtgt tgggaagcga gcctagtcgc gcagggatgc ttgaccataa
    13321 aaaagcacga aaagagatcc aaaaagtttc tgaacagtat ggattgtcag tcaacccaga
    13381 tgcttacgtt cgtgatattt cagttggaat ggaacaacgg gtagaaattt taaaaacact
    13441 ttaccgagga gcagatgtac tgatttttga tgagccgaca gctgtattga cccctcaaga
    13501 aattgatgaa ttaatcgtga tcatgaagga attagtcaaa gaaggcaagt cgatcatttt
    13561 gattacgcat aagttagatg aaatcaaagc agtagctgac cgctgtacag ttatccgccg
    13621 tggaaaagga atcggtacag tcaacgttaa agacgtaacc tcacaacaat tagccgatat
    13681 gatggttgga agaacggttt cattcaaaac gatgaaaaaa gaagcgaagc ctcaagaagt
    13741 cgttttgtct attgaaaatc tagtggtgaa agaaaatcgt ggattagaag ccgtgaaaaa
    13801 cttgaactta gaggttcgtg ctggcgaagt acttggtatc gctggaatcg atggaaacgg
    13861 gcagtcggag ttgatccaag ctttgactgg tttgcgaaag gcagaaagcg gacatatcaa
    13921 gctaaaaggg gaagacatca ctaataaaaa accccgaaaa atcactgaac atggtgtagg
    13981 acacgtgcca gaagaccgtc ataaatacgg gttagtccta gatatgacat tatctgaaaa
    14041 tatcgcacta caaacctatc atcaaaaacc ttacagtaaa aacggcatgc tgaattattc
    14101 agtaatgaat gaacatgcca gagaattgat cgaagaatat gatgttcgaa caacgaatga
    14161 acttgttccc gcaaaagctt tatcaggcgg aaatcagcaa aaagcaatta tcgctcgtga
    14221 agtcgaccga gatcctgatc tgttgattgt tgcaaatcca actcgtggac tggatgtagg
    14281 tgcgatcgaa tttattcata aacgtctgat cgaacaaagg gacaaataca aagcagtgtt
    14341 actgatcagt ttcgaattag aagaaatttt aaatgtttcg gatcgtattg ctgttatcca
    14401 tgaaggagaa attgtcggta tcgttgatcc gaaagaaaca tctgaaaatg aattaggtct
    14461 attgatggcg ggctatacct tagaagaagc acgaaaagag ttagaaaagg gggctggcgc
    14521 acatgaatga tcgcagcgaa aaattccgta atttattagt gcctgtactt tcagttgttt
    14581 taggattgat cttaggcgcg atattgatgg ctgcttttgg ttttcagccc atccaaggtt
    14641 attcttctat gctagatgca gcccttggtt cgcaaagagg gataggtgaa acgttaaggc
    14701 aagcaacgcc gttgattttt acagcattag gtttttctgt agctaactca gctgggtttt
    14761 tcaatattgg gctttctgga caagcacttt gcgggtggat cgtcagtgtc tgggtggcac
    14821 ttgcgtttcc agatctgcca aaagttgttt tgcttccgtt atgcgtgatc ttaggtgcct
    14881 tagctggtgc gatggcagcg gctgttccag gtttacttcg agcatttttt ggaacaagtg
    14941 aagtgatcgt aacaatcatg atgaactata tcttattata cgtaagcaca tttgctttac
    15001 atgaagtgat gcctgaaagc tatcgctcaa gtttggactc ttccaatatg atcagtcaaa
    15061 atgcctcact gcggttacca tggttaacac aagcttttgg cggctctcgc gtaaactcag
    15121 gattattttt agcaatcatc gcgcttattt tcgtttggct gctgatgaag aaaacgactt
    15181 taggatttga aatccgttct gttggattga atccttttgc ttctgagtat gcaggaatga
    15241 gtagtaaacg tacgatcgtt ttgtcgatgg tcatttctgg cgctttggca gggcttggcg
    15301 gtgtggttga aggattagga acgtatcaaa acttctttgt tcaaacgact tcgctatcta
    15361 ttggatttga cggaatggct gtttccttgc ttggttctgg atcagcaatc ggtattcttt
    15421 tatcagcgat cttgttcagt attttgaaga tcggtggact tggcatgcag acaggagcag
    15481 gtgttccgtt tgagatcgtc aatgtatcga ttgctttgat tatcttcttt gtcgggatca
    15541 acttcctgat tcgtttcgtc atggctaaag tcttccaagg gaaaaaacaa gaagagatcg
    15601 ttgcgacaat agaatcgaaa ccaacgaatc aaaaccatga agggggcgaa ctataatgtc
    15661 gactatcgaa ttaattgcct tgatccttac ttctacactg gtttattcaa caccgctaat
    15721 tctaacgtca ctaggcggaa ctttttctga acgaagcggg atcgtaaacg tagggctaga
    15781 gggaatcatg gtcatgggtg cttttagttc catcgttttc aacttgacga tgagtccatc
    15841 acttggttct atgacaccat ggatcgctat tttagtgggt ggcgtcgtag gtatcctatt
    15901 ttctctgatt cacgcagtag caacagtcag tttgcgtgct gaccatatta tcagcgggac
    15961 agtaattaat ctgatggcac ctgctttagg tgttttcttg atcaaagcat ggtatggtaa
    16021 aggtcaaact gataacatca cgcagaatct ggggtatttt tctttcccag gattagcaga
    16081 tattccggtc attggaccaa ttttctttaa aaatacattc ttacctgctt attgtgctat
    16141 tttgattgct gtcctagctt gggtgatcat cttcaagacg aaattcggct tgcgcctccg
    16201 ttcagttggt gaaaatcctc aagctgccga cacattaggt atcaatgtat atgctatgcg
    16261 ttattcaggc gtactgattt ctggcttgtt aggcggtatg ggcggtgctg tttttgctca
    16321 gtcgatttcc ggtaactttt cagctggtac gatcgttgga caagggttca tctccatggc
    16381 tgctatgatc tttggtaagt ggaatccttt aggggcaatg ggtgcctcat tgttctttgg
    16441 ttttgctcaa agcttgagta tcattggcgc acaattacca gttatttctt cgattccgcc
    16501 agtattgctg caaattgctc catatttgct gacaatcatt gtgctggttg ttttcttagg
    16561 gaaagcttct ggtccaaaag caaacgggaa gaactatatc aaatcaaaat aatcttttag
    16621 cgaataaaaa gggtgggtta cgggaagtgt ttttcgtctc gccctttttg ttttaaaggc
    16681 aataaaaata aaaagaaaga aggaataatc atgtttaaac gcgtacatct gatcgtcatg
    16741 gattcagtag gaattggaga agcaccagat gccgataagt tcggtgatgt tggttctgat
    16801 acattaggac acattgcgaa agaagcagga ttgaacgttc cacatttaga gcagcttggt
    16861 ttaggaacga tccgtcctct tgaaggtgtg gaatcagtga aagatcatca aggctatgcg
    16921 acgaaattag aagaagtttc tgtagggaaa gatacgatga caggccactg ggaaatcatg
    16981 ggattgaata tcaaaactcc atttcgtgtg tttcctaatg gattccctga tgatctgatc
    17041 aaacaaatcg aagaattttc tggccgtaaa gtcgtatgta acaaacctta tagcggaaca
    17101 gctgttattg acgattacgg tgaacaccaa atgaaaacag gcgatttaat tgtatatact
    17161 tctgccgatc ctgtccttca gatcgcggca catgaagaaa tcattccttt agaagaatta
    17221 tatcggattt gccaattcgt tcgtgatata acaaaagatg atccgtatat gattggccga
    17281 atcattgcac gtccgtacgt gggtgaacca ggagacttca cacgaacaag caatcgccat
    17341 gattatgcgt tgaatccatt cggacacaca gtccttgatt ctttgaaaga agcaggaaag
    17401 gacgtgattg ctgttggaaa gatcaacgat attttcaacg gacaaggaat cactgaatct
    17461 gtccgcacga aaagcaatat ggatggcgtt gaccagctgc tgaaagttat gaaaaacgat
    17521 ttttcaggaa tcagctttac caatttagta gattttgatg cattatacgg tcatcgtcgg
    17581 gatgtagtcg gctatgcgca tgcgattgaa gaatttgact tacgaattcc agaattgctt
    17641 gaagcgatgg aagaagacga cttgctaatg attacagccg atcacggaaa cgatccgacg
    17701 tttactggta ccgatcatac gcgagaatat gtaccgttat tggtttacag taaaaaaatg
    17761 aaagaacaag gggcattgcc tcaaggatat tattcagata tttcagcaac gattgctgag
    17821 aactttagtg tttcttcgac tgaaaatggc gaaagcttct tgaaattatt aaaataatct
    17881 ttttaaatag gagaaagcaa atgaccaagt taagcgagat gttacaagaa acgacccagt
    17941 ttatccttga taaaggagta aaagaaattg attttggttt gattctaggt tcaggattag
    18001 gtgaattagc agatgaaatc gaagatgctg taatgatttc ttatgatgag atcccgttct
    18061 ttcctgtttc gacagtagtt gggcatgcag gaaagctagt ttatgggtct ctggcaggca
    18121 agaaagtgtt agccatgcaa gggagattcc actactatga aggtcattcc atgcaaacag
    18181 tgacctatcc agtacgtgtg atggccggac taggggccca ttcagtcatt gtgactaatg
    18241 catgtggtgg tgtcaacgaa tcatttgtac caggagattt gatgctgatt accgatcata
    18301 tcaattttac tggtgaaaat cctttgatcg gtccgaatga agatgcaatg ggccctagat
    18361 ttccagatat gagtcaagct tatacgcctg actatcaagc aacagcaaaa gcagcagcag
    18421 aaaaactagg tttggatctt aaagaaggcg tatatatggg ctattctgga ccaacttatg
    18481 aaacgcctgc ggagatccga atgtctcgga caataggagc cgatgcagta gggatgtcga
    18541 ctgttccaga agtcatcgta gcagctcaca gcggattaaa agtgttagga atctcttgta
    18601 ttaccaactt agcagcaggt atgcaagcga atctgagcca tgaagaagtt gtagaaacaa
    18661 ctcagagagt caaacaatct tttaaagcat tggttaaaga aacattgaca ttattgtaat
    18721 aggaggaaca ataaaatgag tgtgcatatt gaagcgaaag aaggcgagat cgccgataag
    18781 attttacttc cgggagatcc tttgcgagct aaatatattg cggagacttt tttggaagat
    18841 ccagtttgtt acaatcaggt acgcggtatg ttaggcttta ccggcaaata taaaggagaa
    18901 cgtatttctg ttcaaggaac aggaatggga atgccatcag ccaccatcta tgcccatgaa
    18961 ttgatccagt cgtatggtgt aaagaaattg attcgagtag gtacatgcgg tgcattgtca
    19021 aaagacgtcc atgttcgtga tttagtacta gcacaaggtg cagcgaccag ttcttcgatg
    19081 atcgaaaaaa atttccaagc ctttcatttt cctccaatca gtgatttcaa tctattactg
    19141 aaagcatatg agatagctaa agaaaagggc tatacggttc atgtaggaaa tgttctttcg
    19201 gaagattctt tctacaaaga tgatttgaca gagacgttcc aattagctga acttggcgtt
    19261 ttaggagtgg aaatggaagc agcggctctt tattacttag gcgcgaaata ccatgtgcag
    19321 acattaagtt tgatgaccgt tagcgaccat ttgatcaccg gtgaagaaac aacagctgca
    19381 gaacgtcaat ctacgttcaa tgaaatgatc gaagtaggat tggaaacagc gatagcagaa
    19441 taagaaggaa atgaaaaagc tgccctgcat taagtaataa gaacatccaa gcaaaaatag
    19501 ctcctcatgt ttttgacttg gctggactta ttattgcaaa tgtaagcttt tgaacaccgt
    19561 ttaccaagag gttgtgaa
//
LOCUS       DACDXE010000059        19455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.059, whole genome shotgun sequence.
ACCESSION   DACDXE010000059 DACDXE010000000
VERSION     DACDXE010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.059"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..3658
                     /locus_tag="IWM23_002051"
     CDS             131..3658
                     /locus_tag="IWM23_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9884664.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3655..7377
                     /gene="addA"
                     /locus_tag="IWM23_002052"
     CDS             3655..7377
                     /gene="addA"
                     /locus_tag="IWM23_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAP9884665.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7446..7787
                     /locus_tag="IWM23_002053"
     CDS             7446..7787
                     /locus_tag="IWM23_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884666.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8096..9139
                     /gene="pheS"
                     /locus_tag="IWM23_002054"
     CDS             8096..9139
                     /gene="pheS"
                     /locus_tag="IWM23_002054"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAP9884667.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9144..11564
                     /locus_tag="IWM23_002055"
     CDS             9144..11564
                     /locus_tag="IWM23_002055"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAP9884668.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IWM23_002056"
     CDS             complement(11734..12744)
                     /gene="trpS"
                     /locus_tag="IWM23_002056"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAP9884669.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12998..13735
                     /locus_tag="IWM23_002057"
     CDS             12998..13735
                     /locus_tag="IWM23_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAP9884670.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13749..14564
                     /locus_tag="IWM23_002058"
     CDS             13749..14564
                     /locus_tag="IWM23_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAP9884671.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14577..15224
                     /locus_tag="IWM23_002059"
     CDS             14577..15224
                     /locus_tag="IWM23_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9884672.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15240..15896
                     /locus_tag="IWM23_002060"
     CDS             15240..15896
                     /locus_tag="IWM23_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAP9884673.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16058..16879
                     /gene="racE"
                     /locus_tag="IWM23_002061"
     CDS             16058..16879
                     /gene="racE"
                     /locus_tag="IWM23_002061"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAP9884674.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16882..18237
                     /gene="rph"
                     /locus_tag="IWM23_002062"
     CDS             16882..18237
                     /gene="rph"
                     /locus_tag="IWM23_002062"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAP9884675.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18238..18756
                     /locus_tag="IWM23_002063"
     CDS             18238..18756
                     /locus_tag="IWM23_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9884676.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18863..19363
                     /locus_tag="IWM23_002064"
     CDS             18863..19363
                     /locus_tag="IWM23_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9884677.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6541 a         3341 c         4178 g         5395 t
ORIGIN      
        1 tttgcgtata cttaaaaaag tagaaatatc agaaccttaa aaggatataa tttgaagaat
       61 atagaacaaa tgttcagtat tttttgatat aataaaggag caccgtatca tgtgttgaag
      121 gaggaaaagc atgtctttac aatttattct tggaaacggg aaaaaagatc accgtcaagt
      181 cttgatcgac gaagcatccg cttggctcga ccaggatgca agtaaccaag tatttttcct
      241 tgttcccaac tacagcaaat tcgaacagga acaagaaatt ttagcggaga tgcgaagaag
      301 aagcggaaaa aaaactttca gcacaacaaa cgttcaagta tttagttttt atcgtctggc
      361 ttggtatttg ctgcaacaga caactcttct tacaggaaat gaattatcag aatcaggatc
      421 tgcaatgatc ttgcgacaga ttttagaaaa aaatgaggaa gaattaacaa tctttcgcgg
      481 agaaatcaat aaaagagggt ttattcgtca attacaagaa ctctacagtg aattgcgtac
      541 aggaaatatt caaccagaag acctgatgct tttatttggt gattcaccta atgcagaaga
      601 ccagcaactt aaaatgaaag acttgaagct gattttttca gcttatgaca gcgaattgac
      661 tcaacgtgca ctaacaaatg aagatgcact atctatacta gcaaattata tgcagacaca
      721 agatttcaac aacgtcaaat ttattgtcag tggtttttct cgaatcaatg gtcaagagta
      781 tcaattactg caggcaatga tggaaaaggg gcaattggtt gtcgatttat tgctggatcg
      841 tccttataca gcggatcttc cagaagtact tacgctcttt catgagactg gaaagcttta
      901 tttccgtctt tttcaaagtg caagagaacg gaaagtccct atattagtgg accgatttgc
      961 accagagcag actcgtccga cagagcttca actgctgcaa acaatgtggg aagagacatc
     1021 tagccaaaag aaagcggcag tacacaaaat agggggacaa gagcatctcc atttgtggga
     1081 agctgaaaat ccaactgaag aagtccgaca attagctgtt gaaatcagac gactagtcag
     1141 tgaagaaaac taccgttatc gtgatatcca agtgctgaca aaaaatatgt ctttatatgg
     1201 aaatatcctt cggccgatct ttcaagaaat ggcaattcct ttttatatcg atgaagaaca
     1261 aatgatggaa aatcatccgc tgatcgaatg gatcaacagt ctgttcgctc ttgaccgcta
     1321 ttcttatcgg ctaagtgata ttatgcgttt tttcaaaact gaattatttc tgtcgacgga
     1381 agcagaaacc gaacttgatg aatggaaaca agagcgagat gaattcagga aaaaactgga
     1441 catcactgaa aatgtagcac ttgcttacaa ttatcaagga acttattgga ctcgtgaaaa
     1501 agactggcag tttgtggcgt atgattttga agcagaacag attgaagacg tacagtattt
     1561 ggaagaacag tcaaatacga ttagacgatt atttcagaaa gctgtaccag tatttttgaa
     1621 aaaaataaag aaaagcaaaa caggacaaga ggccgcaaat gttttttatc aatttttgat
     1681 ccaaagtggc gtacagcaac aattattgtt ttggcgtaat caggaagtag acagaggaaa
     1741 cttagaagcg gcaagaaacc atgagcagac atggggagcg ctgattgatc tgttggatga
     1801 atatgtagta atttatggtg aatcggcttt tgactggtca ctgttccaag aaatcattac
     1861 aagtggtcta gaaaatctaa gttatggtaa aatccctacc gcaatcgacc aagtgcgaat
     1921 caatcgtttg gaattagttc gaccaaatca agctgccgtc acttttgcat tagggttgaa
     1981 tgatcaagtg tttccagacc gaaaagagac aaaaggattg ttgtccagtg aagaacgaga
     2041 agtattaaat caggccttgc cagatggaca gtttttattc gacccatcaa aggaaagtat
     2101 ctcttttgag cctttccaag cctatcttgt ttttctttcc ggaacagatc gattatatct
     2161 tagttatgca caaagctatg acacagatgg ttcgctgaag atgtctcctt atttgcgtag
     2221 aatcacagaa tatttatctg ttcccgtcga gagaaaagag catttgacga tcgaaagcga
     2281 ccctaattgt tatgtaggga cttacagaag cggaatcaat acagtcaata ggatttatcg
     2341 attagcttta gatgaaaaaa aacctgtacc aaagcactgg caaaagctca aagaattagt
     2401 actgaattcg tcatggcgaa attttgcttt acagattttt gaaagtcagg aacatttaaa
     2461 tgttcctgtt tcgatgccag aaaaaatggc ggaagagctt tatggaaaag agatttatac
     2521 ttcaatttca cgtatggaga atttttataa ttgtgaatac aaatacttca tgcaatttgg
     2581 tttgaaattg aaagaacgaa ctgtttatgg tctaactcca gcagcaactg gtgattttta
     2641 tcatgaatca ttggatcgtt tcttcaagct actgttttca aaccaattat ccttagtgaa
     2701 tatgtccgat gcgcagcgta gagactttac tgaaaaagtg ctgcaggaag tattcggaga
     2761 acttcggttt gagatattgg atagttctgc aagaatgaac tatatccgct accagttagg
     2821 acagacgatt caaaaagtga catgggcttt gcaaaaacag agcaaaaata caggcatgca
     2881 tcctcttcag acagagattc tatttggtca gattgctgga gcaaaaggta tcacaggtct
     2941 ggaattaccg ttgagtaatg gtggaaaact ccatgttcga gggaaaatcg atcgaatcga
     3001 tgtagcttct gaacaagaag atacttggct aagcgtcgtt gattacaaat ctagcggacg
     3061 ttcattcgat gtcacagaag cttattatgg catggctatg caattattga cgtatctgga
     3121 cgtagcattg atggatgcgg tacgtttgac tggaaaggcg gctgttaaac cagcaggatc
     3181 atattattta catgttcata atcctgtatt gacggagaca gatgatgtag agaagaatac
     3241 attgaaaaaa tttagttatg atggcgtttt tgtcgatgat cccgctcttt tagaagtact
     3301 agatcattca ctggaagcaa aagaacaatc actgatctat ccagtgaaaa aaaatgcgaa
     3361 agatgaatac caaaaaggta gtttcagtaa agacaaattt ttcacggaag aagaactgca
     3421 actattcatg gcgcataatc gggaaaatat gcggttagct ggagatgcga ttacttccgg
     3481 agaaatcaaa ctaaatccag cttataaaga taaacagcgg attgcctgcg aattttgtcc
     3541 attcagaagc atctgtacgt ttgatgtgat gctgaaagaa aataattacc accgtctgga
     3601 gaaaatagat aaggaagaag cactgcgtag aatattgaaa aggagtgaag aagagtgaat
     3661 caaattcctt taaaaccgca aaatgaacga ttcacagatg atcagtggca agcgattttt
     3721 gatcaaggag ataatctatt agtctctgcg tctgccggat caggtaaaac tacagtgctt
     3781 gttcgaagag tgattgaaaa actaaaaatg ggttttgata tcgatgaatt attgattgtg
     3841 acatttactg aagctgccgc aagagaaatg aaagaaagaa ttcaagaggc attacaagaa
     3901 tccgtcaata gcgaaagtga ccctgtacgt agacagcatt ttactaagca gttagtttta
     3961 ttgcccacag cgaatatctc aaccttacat gctttttgcc tgacagttat tcgacgttat
     4021 tattacttaa ttgatattga tccagtcttt cgcatgttaa cagatgaaac agaaacgatc
     4081 ttgatgaagg aggatgtgtg ggacgagctg agagaagcac tttatgcaga aaatgatgaa
     4141 cgatttttcc aattaacaat gaatttttct aatgatcgtt cagatgacgg cttgacgaat
     4201 ctggtgtttt ctctttatga gtttgcccgt gccaaccctg acccccaaaa atggctagaa
     4261 cagttaagcg ataattatcg tttgcctgag gggctagcga aatctcgatt atatcaaaaa
     4321 caaatccgtc ctttagtttt ggcagatata taccaatgtg tacagttata tgaacagatg
     4381 actcagttag cacaaggtga aggattagaa aaaatgcatg agcaagtagc tggagaacaa
     4441 cagcagatca agaatatcta tgaagcgttc tcgcaagatc ggttagaaga agcttatgct
     4501 ggtttggaac aactaacttt ttcaactttt aagagcagtc ggaaagcaga attgaaagaa
     4561 atttccaatg aagtcaaggg catgagggat aaagcaaaaa aactgattca gcagataagt
     4621 aaaagttatt ttcctgtttc cccaagccaa atggaagaat taacggacaa agcactaccg
     4681 ttagtcgaag aaatgacaaa agtcacgcaa agcttcatgg acggtttcag tatgcgtaaa
     4741 cgggaaaaag gtgtgctgga tttcaatgat ctggaacatt tagcgttgca gattctcaca
     4801 gaaaaaacga aagatgtatg gctgccaagt gaagcttcaa aacattatcg aaaaaaattc
     4861 aaagaagtaa tggtagatga atatcaagac gtgaaccaat tacaagaagc cattttatac
     4921 tggctaagag aacccgatga tacaaaagga aatatgttca tggttgggga tgtcaaacaa
     4981 tccatctatt ctttccgttt agcagatcct agtttattta tcgggaaata cgagaatttc
     5041 tcaaagaaag aaggcggacg tcgaatcgtt ttggctgaga acttccgctc tagaaaagaa
     5101 gtcctttctt ttacgaatct tatttttgaa caactaatgg atccagcagt gggtcaaatc
     5161 aattatgacg aagcagccaa actgatccaa ggattttcag actttccaga gaatgaacag
     5221 tttgagccgg aaatcatgat ctatgaaaaa gaacaggaag aatcagagat tgagattccg
     5281 acggatgata tactggaaga caaaacagaa ggcgaacttt ttatgacagg actaaaaatt
     5341 cgtcaattaa ttgattcctc ctttatgatt tatgataaaa aatcaaaaaa aagtcgtccg
     5401 atagagtaca aagacattgt tctcttgaca cctacaaaga agaacaactt aacgatttta
     5461 gagattttca aaacattgga tattccatta gagatgaatg atgctcaaaa ctattttcaa
     5521 gcgacagaaa ttcgcacgat gatctcgctg ctacaattaa tcgataatcc ctatcaagat
     5581 attccgcttg cggctgtatt gcgctcaccg atcgtgggtc tgattgaacc agaattagcc
     5641 agcattcgat tagctaaccg tgcacatact tactatgatg ctgtgcttgc ttatcaagca
     5701 tcaaacgaag atgaattagc agctaagctg gaacattttg gcaagcaact agagcattgg
     5761 cgagaacttg ctcgccgttc ttcgatcact gacttgttat gggatatcta ttatgagacg
     5821 ggatatctag agtatgtggt cggtctgcca gcaggggcac agagacaagc caatctttat
     5881 gcattggttg atcgtgccaa agcttacgag caaagttctt tcagaggatt ataccagttc
     5941 gtgcgtttca ttgaaaaaat gcaagaaaaa gataaagact tagcagaacc agtcatttca
     6001 atagaagata atgctgttcg cgttatgacg atccatgcaa gtaaaggctt ggaattccca
     6061 gttgtatttt tgttagatat gacaaaggag ttcaacttac aagacttgcg caatcggtat
     6121 gcttttgaag aaaaacttgg agcaggaatt cgctatatgg atcctgaaac acgagtgtta
     6181 tatgatacgt taccgtttca agccatcaaa ttggcaaagc aaaacaaatt gttgtcggaa
     6241 gaaatgcgta aattgtatgt aggattgact cgggcagaac aaaagctttt cattgtcggt
     6301 tcctataaaa ataaagagca aatgatccaa acgtggtctg aagcagctga tcatgaggag
     6361 ttagtttttg atcctgcgct acgtttgaag ggcagaagca gcttgatgaa ttggattggt
     6421 tacggattga tccgtcatcc tgaaatgcag aaatacttag aggaagaaat ttcgacttct
     6481 cttcttcaac attcaaatgc acagttttct atttcatgga tgaatcagca atcgatcatt
     6541 gaacaaagac agttgcttgc tgaaaaagaa ctggttaact tagatcagca gatgaaagaa
     6601 gatgaaactt tattagcaga ttcgttacaa aaacgtctgg catatgaata tccttatcaa
     6661 gcttcttcac aaaccacaag ttatcaatct gtatctgaaa tcaaacgatt attcgaagat
     6721 ccagatgata cacaagaatc acgtttgact ctggaaagca gtcagaacaa agcagcatcc
     6781 cgtcaatttc gttatacgca agaacaacta gcagaaccaa aatttttgca aaaagatcgg
     6841 caagtgtcag cagctgctgt tggaactgct acgcatgctt tgttacaatt actgccatta
     6901 gagatgccaa ctacagagag tatccatcaa aaattacagg aattggtaag gaaacggtta
     6961 gtagacgaaa aagtcgcgaa aaaagtagat gtttcttcta ttatatggtt ctttcaaaca
     7021 gaacttggtc aacagctgat tgccaacaaa gaaaacgtaa aacgagaaca acctttttcc
     7081 atgctgcttc ctgctgacga agtatttcaa gactatccaa atcaagaaga tgaactatta
     7141 atccacggaa tcgtcgatgg atacctagaa gaaaaagatc atctcaatat ctatgatttt
     7201 aaaacagatt ttattttaca tccggatgat ccagcagaaa tagatgcaat cgtccaaaaa
     7261 tatcaaggac agctaagact ttaccaacaa gcaatgtcag aagctttgaa taagccagta
     7321 gaaaatgttt tcctaatttt gcttcgagtg aaacaaatta taaacattaa caaataatta
     7381 taattgagca aacttgactt atgttttgta aggattatgc tatactctgg gaagaggtga
     7441 gtatgatgga gctaacacag acagaattcg ttttatgccc taaatttgaa aaaacattct
     7501 ctattttagg aaaaaaatgg aacggattga ttatcgatgt actactcgaa aacggtccgc
     7561 aacgctttgt tgagcttgcg gcaaaaatcc cgatggtcag tgatcgagta ttagtcgaac
     7621 gcttgaagga attagaaaaa gaaggaatag tcactcgaaa ctttgcctgt agcgaaaaga
     7681 gccgttcaga atatctcttg actgaaaaag gagcagactt gcaaaaagcg atgcgtcaaa
     7741 tacaagtttg gagcgaaaaa tggatcacag aggaagaatg cggttgattt tgtaaaaaaa
     7801 atcttgtaaa ctatagtcca tacgaataag taaagaacga tgacgaaaaa gagtagtttg
     7861 gctaaaccag ttcagggaga tctgtcgcag actgaaagca gatcctgtgt ttgaaaaatg
     7921 aagttcactt tccgagttcg tctgataaaa tcagaccggc aattgtcgtt aacgaagtaa
     7981 gagtgcggtg taagtcattt atccgcaaat taggatggta acgcgaagac ctcgttcctt
     8041 aacaggaatg aggtcttttt gttttgtatt tggaatttaa aggaggaaca agcacatgtc
     8101 tctacaagca caattagaga gcttgaaaga agcaacgctc gtcaaaatca cagcggcaca
     8161 agatttaaat gaattaaatc aaatacgtgt tgaaacgtta ggaaaaaaag gaccgatcac
     8221 agaagtatta cgtgggatga aagatctaag tccagaagag cgaccaaaag tcggaagttt
     8281 cgcaaatgag atccgagatc ttttgacaga agagatcgag cgaaaaaaac aagtattaga
     8341 agaagaagcg ttaaatgcag ctttggcaaa agaaacaata gacgtgacac tacctggtag
     8401 aaaagtaaaa catggtacac gtcatatcgt gacacaaatc atggaagaaa ttgaagatat
     8461 ttttgtgggt atgggctatc agatcgtaga aggatatgaa gtagaatctg atcattacaa
     8521 tttcgaacgg atgaatctgc caaaagatca tccagcaagg gacatgcaag atactttcta
     8581 tatttcagac gagatattga ttcggacaca tacttcacca gtccaagcac ggacaatgga
     8641 aaaacatgat ttctctaagg gtgctctacg aatgatctcg ccagggaaag tcttccgcag
     8701 agatacagat gatgcgaccc acagtcatca gttccatcaa attgaagggc tagttgttga
     8761 taaaaacatc acaatgggcg accttaaagg aacattagaa gtcgtaatga aaaaaatgtt
     8821 tggggaagat cgtgaaattc gtttgcgtcc aagttatttc ccatttacgg aaccatcagt
     8881 agaagtagat gtcagctgtt tcaaatgtgg tggtgccggt tgtaacgtat gtaaatacac
     8941 tggatggatc gaaattttag gagctggcat ggtacacccg aatgtgttga agatgtcagg
     9001 aatcgatcca gaagaatatt caggctttgc tttcggcctt ggaccagacc gcgtagctat
     9061 gttgcggtat ggagtgaatg atatccgtaa tttctatcaa aatgacttac gtttcttaag
     9121 tcagttcaag ggggagtaag aaaatgttag tttcatataa atggctcaat cgttatgtag
     9181 atttatcgaa tgtaacacca aaagaattag cggataagat gtctgtgaca ggaatcgaag
     9241 tagagggagt aacggttcca gaagaaggct tgaaaaaaat cgttgtcggt gaagtcaagg
     9301 aatgcgtacc acatccagat tctgatcacc tttctatttg tcaagtcgat attggcgaag
     9361 acgaactgtc tcaaatcgtt tgtggagcgc ctaatgtgaa ggttggaatc aaagtgattg
     9421 ttgcacttcc taactcacgt atcgctggta atgtaaaaat caaaaaagga aaaatgcgtg
     9481 gccaagtatc aaatggcatg atttgctcat tacaagaaat tggctattca gatagtgtta
     9541 taccgaaaga atacgctgaa ggtatctatt atatgccaca agaagctgta aatggcgatc
     9601 cagtttttcc ttatttggat atggatgatt cgattattga attatctatc acaccgaatc
     9661 gtgcggatgc attgtcaatg cgtggcgtag cttatgaagt tggggcaatt tatcgtcaga
     9721 ctcctacatt cgatgacgaa aagctggtag aatcctcacg tcctgctagc gataaaattc
     9781 aagtggaagt tgaagataaa gaagcagttc ctgcttatca aatcagaatc attgaaggag
     9841 taagaatcca gccaagtcct caatggctgc aaaatctttt gatgaacgaa ggcattcgtc
     9901 caatcaacaa tgtagtagat gtgacgaact atattttgct tttattcgga caaccactac
     9961 atgcatttga ctacgacaaa ttgaaatcaa aagagatctt tgtccgccgt gcaaaagaaa
    10021 atgaaacatt agtaacacta gatggagaat ctcgggaatt gacaactgac aatctcgtca
    10081 ttactaacgg caaagaacca gttgctttag ctggtgtcat gggagggtta gactcagaga
    10141 tcacgaatga aacaacaaca gttgcactag aatctgcttt atttgatcca atttcgattc
    10201 gtcgtacagc aaaacaattt aatttgcgta gtgaatcctc tgcacgtttt gaaaaaggaa
    10261 tcaaccatgc gactgttgga gaagccagtg aggttgctgc agcaatgatc gtttctcttg
    10321 cgggtgggga agttctacaa ggcgcggtca aaggctcaga gatcaaagca gaagacgtat
    10381 cagtgaagat ttcacttgaa cgcatcaatc aatatttagg aacatctctt gtcgttaaag
    10441 aagtaaatga gatttttgaa gcacttggat ttagctatga agaaaaggag tatgtttatt
    10501 ccgtagtagt accaccaaga cgctgggata tccaaatcga agcagatatt atcgaagaag
    10561 ttgctcgtat ttatggatac gaccagttgc catctacact tccaagtgga gaaacggttg
    10621 ctggaagctt gtcaaaagaa caagaaacta ctcgtaaaat tcgtacaatc tttgaaggaa
    10681 gcggattgag cgaagcaatc agttatgcat tgacaacaga agaaaaatca agacagtttg
    10741 ttataaatga aagtaatatt acacgtttgg actggccaat gagcgaagaa cgttctgtat
    10801 tacgaatgaa tttgatcagc ggactattag atgatatcag ttataacact gcccgaaaaa
    10861 atacagagat tgctttatat gaaattggtc gggtgttctt ccaagaaaat gatccacgta
    10921 cacatctgcc gcaagaagta aaacatgcag caatcgctat ttctggtatt tgggaagaaa
    10981 aagattggca gacgaaagct caaaatgtag actttttcac catcaagggt cttttagaaa
    11041 acttatttga acaactaggc atcactgaag atgtccatta tcaaacagtg acagaaatga
    11101 aggaaatgca tcctggaaga actgctgcga tttaccttgg cgaaaaattt attggttttg
    11161 tcggtcaagt gcatccaacg attacaaaaa gttatgatat tccagaaaca tatgttgctg
    11221 agtttgattt atctgctata gtggaagctg ctcaaaaagg gatcgttttc gaagcagtga
    11281 caaaattccc agcagtcagc cgggacatcg ctttgcttgt aaaagaaact gtgacaaatc
    11341 aggaattaac agaagttatc cgttctgctg ccggaagatt tttaacagat atccaattat
    11401 ttgatgtcta tcaaggagaa aacattgaaa aaggacataa atccatggcg tatagcctga
    11461 cattcgtcaa cccagaagca acattgacag atgaagaaat caataaagca atggaaaaag
    11521 tcgaaaaagc gttggtagaa aacctagagg caagtattcg ctaagaagga aaatatagta
    11581 agtaacgatc aagtagcttt tgaagaccat acatttgaaa aaaagatgtg acacaacttc
    11641 gtcacatctc tttttttgat tttaaaaaag gaatagcaca gagacgagat aaaaaaacag
    11701 gcctttacag cctgcttttt tagagtaaat gaattaatgg aagtaattga ttcccattgc
    11761 tgattttact tcagaaagtg tttgtgcagc tactttagca gcttcttcac tacctttacg
    11821 taagatttcc ataacagctg aaggatcttt ttccaattct tctcggcgcg cacggatcgg
    11881 tgcaaattca gcttccaata catcaatgag gtatcgtttg atctttacat caccaagtcc
    11941 gccgcggcgg taatgagctt tcatttcttc aattgcttct ttatctgttc cgaatacatc
    12001 caagtaggtg aagaccatat tcccttctac ttgtccagga tcttgtacat ggatgtggtt
    12061 tggatcagta tacatactca tgactttttt ctgtaagaca tcggcaggat cggagatata
    12121 aataccgttt cctaacgatt tactcatttt gccatttcca tcgattccag gtaatctacc
    12181 cattcctttt ggtgggaata cgccttttgg ttccaccaag acttcaccat atgtatgatt
    12241 gaagctttgt acgatttcct gtgtctgctc tagcattggt ttttgatcct caccaacagg
    12301 tacaagattc gctttgaatg cagtgatatc tgctgcttga gaaacaggat agataaagaa
    12361 tcctgtaggc acgctttcac cgaatttttt ttgttcgatt tctgttttga cagtcggatt
    12421 gcgccgaacg cgtcctacgc tgactaagtt caaataatac atggttaatt cagctaattc
    12481 cggaatttgt gattggataa ataaagtaga ttttgcagga tctaatccca cggctaaata
    12541 gtctaaagca acttgcaaga catttgatga aaccttctca ggatttttag cattatctgt
    12601 cagtgcttgc atgtcagcaa tcatgacaaa cagttggttg tcttcatctg cctgcatagc
    12661 cactcgggtt tttagagaac cgacgtaatg ccctaaatgc agcttcccag ttggtcgatc
    12721 tcccgttaaa ataatatttt tcaaaaaaga cacctcttta cgatttgtaa gtctgagcac
    12781 ttaatatctt accgtttttc ctgaaaagaa acaacagttc ctaaggaaaa aacaaggaaa
    12841 gaggataaaa taactaatag attagaaatg gaagaaatat ttatgggaga ataaacgttc
    12901 taagaaactt tacaaattac taaactatca gatataatgt attttattat acatctttaa
    12961 aagaaatctt actaaataat aatgggagtg taattctatg gcaatgatta aatttgatca
    13021 cgtggataaa tattatggca agttccacgc attaaaaaat atcaatctcg aattcgagaa
    13081 aggagaagtt gtcgttgtca ttggtccttc tggttcagga aagagcacga tgcttcgctg
    13141 tatcaatgga ttagagacga tctcttcagg taagctgctg attaatgata ctgacttgca
    13201 tgataaaaag acaaaactga cagaagtccg aaaaaacatc ggcatggttt tccaacattt
    13261 caatttgtac ccaaataaaa ctgttctgga aaacatcaaa ttagcaccaa tcaaagtgtt
    13321 gaagcaagac caggcaactg cagtcaaaaa cgcggagaaa tttttgaaaa ctgtcaatat
    13381 gttagataag aaagattctt atccttccat gttatccggg ggacaacagc aacgggtggc
    13441 aatcgccaga ggtttagcga tgaatcctga gatgctgctg tttgatgaac ctacttctgc
    13501 tttggatccg gaaatgattg gtgatgtatt agacgttatg aaaaaattgg ctcatgacgg
    13561 tatgtcaatg attgtcgtga cacatgaaat gggctttgcc aaagaagtgg cggatcgtgt
    13621 tatcttcatg gctgatggag aagtattgga agacagcaga aatgtgagag actttttcga
    13681 aaatccaaat gaagtccgag cacagcaatt tatcagtaaa gttatcaatc attgatagga
    13741 ggaaaaagat gcgcaggtta aaaaaaatac gcctgatttt ccttttaggc attgcttttc
    13801 ttgcactagc cggatgtaaa ggaaatgggt tggcaaatga agatatttta gagcgaagca
    13861 aatctaccaa tgaaatcatc tggggtgtca agtatgatac tcgattattt ggaatgatgg
    13921 atattgagag cagaactgta caaggatttg atgtagatat tgcaaaagca atcactaaaa
    13981 agatccttgg tgacaatgga aaaacagaat ttgttgaagt cacttctaaa actcggattc
    14041 cattattgaa aaacggaaat atcgatgcaa ttattgcaac gatgacgatc acagatgaac
    14101 gaaaaaaaca ggttgacttt tcagatgtct atttcgatgc tggccaagct ttgttggtaa
    14161 agaaaggaag ccaaatcaaa agtgtagatg atttgaatgc ttctacaacg gttctagcag
    14221 tcaaaggttc tacatcggct gcgaatatcc gacagcatgc accagatgca aaaattttag
    14281 aattagaaaa ctatgcagaa gcatttactg ctttacaatc tggtcaggga gacgcgatga
    14341 ctacggataa tgcgattttg ttagggattg cagatgaaaa tccagagtat gagttagttg
    14401 gtggaacgtt tactaatgag ccttacggga ttgcgatcaa caaagggcaa gaaaacttct
    14461 tgaaagctgt taaccaagcg ctagaggaaa tgcatgcaga tggcacatac gataaaatct
    14521 atcaaaaatg gtttccaaat gaaactgaag gaaaagtaga gtaaggggga gaggcaatga
    14581 gcgaattgat acaaacttat ggaccaatct ttgtcgacgg gttcaagatc acattgtttt
    14641 cgagtgtctt agcacttgtg tttagtttga ttatcggaac gttaatggca atcttccaat
    14701 tatcaaagaa taaagtagtg agcgccttag caaaggcata tgtagaattt tttagaaaca
    14761 ttccattatt aatcatcgtg atgttttttt atgttgtcgt accgatttat tggtttaatc
    14821 tagatggatt ccaagcaggt acgattggat taacgatcta tacatctgct tttattgctg
    14881 aaacagtacg ttcagggatt caaaccgtac caaaaggtca aacagaagca ggactatctt
    14941 ctggttttac ctattcagaa atcatgcgtt atattgtttt accgcaagca ttcaaaatcg
    15001 ttatcccgcc attaggcaac caatttatca acctcgtaaa aaactcatct attttagcaa
    15061 tcgtagctgg tttagatttg atgtatcaag gagacttgat cgctagtgaa acattcaata
    15121 ccttcgatac gtatatcatc gttgggttat tttacttagt tatcacgcta ccgttatctt
    15181 acttaatggt ttatcttgag aaaaaatggg cggttcgttc atagaaggag gggaaataca
    15241 tggattttag cggcgctttt tcatggatga atatccgttt tttattggaa ggattaaagg
    15301 ttactgttga agtttcattg ttttcgatca tttttagttt cttgatcggt ggtctgacag
    15361 gtgtgattcg ttttgcgaat atccctttct tttccaaaat ccttggtgta gtgattgata
    15421 ttatacggaa tcttccatta ttattgatca ttttcttcac ttactttgcc ttacctcaaa
    15481 tcggcattca gatgaacatc ttctggtccg ctgtcgcagc attaacgatt tttgagtcag
    15541 ctatgttgtc ggaaattttc cgagctggac tcaatgctgt tccaaaagga cagatggaag
    15601 caggattatc aacgggactg acttatgtag aatcgatgcg aacgatcgtg ttaccgcaag
    15661 catttaaatc aatgatcccg gcaattgtga gccaattgat ttcactgatc aaagatactt
    15721 cattagcagt aatcatttct ttacctgaac tttctcatca ggcacgtatt atttatggac
    15781 agaacaccaa ttatgtgctt ccgatgttca tcatcatgac acttatgtac ttcgtggtat
    15841 gttacgcgct ttctctgctt tccggttact tggaaaagag acggtacagt tattaataga
    15901 tgattctttt agcggttgtg gcaaactaaa gtcacaaccg cttttcctct ttccattcga
    15961 aaaaaagaga tttcgattgt tttaagcaag taagagcact tgaaaattca cgctaaaaag
    16021 tacacaatag ggaggataga atttattgaa aaaaaggatg atacgattga cagataatcg
    16081 ccctatcgga tttattgatt caggtgtcgg cggcttgact gtagtaaaag aagccctgaa
    16141 acaattaccg aatgaaaata ttttatttgt aggagacaca gcacgctgcc catatggccc
    16201 tagacccgcg gaacaggtaa tccagtatac ttgggaaatg acggattatc tggtggagca
    16261 aggaatcaag atgctggtga tcgcctgcaa taccgcaact gcggtggctt tagaagaaat
    16321 caaagctgct ctttctattc cagtcatcgg tgtgatcctt cccggtacta gagcggcagt
    16381 aaaaaaaaca caaaataaac aagttggcat tatcggtacg attggtacgg taaaaagtca
    16441 agcttatgaa aaagcactga aagagaaagt accagaattg actgtgacaa gtcttgcttg
    16501 tcccaaattt gtttcagttg tcgaaagtaa tgaataccat tcatcggtgg cgaaaaaaat
    16561 tgtggcagaa acattagctc ctttaaccac taaaaaaatc gatacattga ttttgggatg
    16621 cacccattat ccattattac gccccatcat tcaaaatgta atgggagaaa atgttcaact
    16681 gatcgattct ggagcagaaa cagtaggtga agtatctatg ctgttagatt atttcaattt
    16741 gagcaattca ccgcaaaatg gtcggacatt atgccagttt tatacaactg gctctgccaa
    16801 acttttcgag gaaatagctg aagactggct tggaatcgga cacttaaatg tagaacatat
    16861 cgaattggga ggaaaataag gatgcgtcat gacgggagaa aagcccaaga gcttcgcaaa
    16921 ctggagcttc aaacaaatgt tttcaaatat ccagaaggat cggtagtgat ccgttttggt
    16981 gatacaacag ttatttgttc tgcaacaata gaaaattctg ttcccccttt tttgagagaa
    17041 acaggaacag gatgggtgac agcagaatat agtatgctgc ctcgagcaac gagtacgaga
    17101 aatcgtagag aaagcagcaa agggaaactt tctggtagaa caatggaaat ccagcgtctt
    17161 attggtcgtt ccttgcgtgc agtagtagac ttggaaaaat taggagaacg tagtatcatc
    17221 gtagactgcg atgtgatcca agcggatggc ggtactagaa cagcaagtat cactggggca
    17281 tttgtggcat tgaaattagc cattgaaaaa cttttaagag aaaaagaatt gtcagaagat
    17341 ccaataaaag aacatcttgc tgcagttagc gtaggaatct taccagatgg gacttgcgtc
    17401 acagatttgg attaccaaga agattccgca gcacttgtag acatgaatct tgtaatgaca
    17461 gaatctggaa aattcgtgga gattcaagga actggagaag aagcaacgtt tgatggtgaa
    17521 cagttgaatg aaatgctctt ttttggaaaa aacgcgattg aagatttgat caaagagcaa
    17581 aaacatgctt tgctaacaga gtttgctcaa aatgatgagc ggatagaaga aacaaaaaca
    17641 atcatcattg cgacaagaaa tccaggaaaa gcagaagagt ttcgaaatat gttcaaagaa
    17701 gcgggatatc atgtaaaaac attacttgac tatcctgaat tgccagatgt ggaagaaact
    17761 ggcagcacat ttgaagaaaa tgcccgatta aaagcagaaa cgattgcaca attgcttgat
    17821 cagccagtct tagcagatga ttcaggtttg aaagtggatg ctttaggcgg tatgccagga
    17881 atctattctg cacgttttgc aggggaacaa aaaagtgatg cggggaataa cgccaaatta
    17941 ttatatgaat tgacagatgt gccggatgaa aaaagaacag cacaattcca ttgtacgtta
    18001 gtattcgcgg cacccaaaaa agacagcctt gtagtagaag cggaatggcc cggtcgagta
    18061 gcccgtatac catctggcga aaatggcttt gggtatgatc ctttatttat cccagaaggt
    18121 aagaaacaaa cagcagcaga attatcgagc gaagaaaaaa acaagatcag tcatcgggct
    18181 caagcaatga aaaaattgag tgccgagtgg aaacaatggc tggaaggaga gagatagatg
    18241 cggtatttag tagtaagtga taatcatgga gacagagata tcctcgttga cttagtagat
    18301 cgttatcgtt ctgaagtgga tatctttttc cattgtggtg attcggaatt agaagcttct
    18361 gacccattat gggatacatt ccaagtggtt caaggtaatt gtgattatgg accaggattc
    18421 gaacagaaaa aagtgatcca aactggtcag gatacagtat tcatgactca cggtcattta
    18481 tcgaatgtcc gatttggttt gacacagtta gcaatcgaag ctcgagctgc aactgccgat
    18541 atggtgtttt ttggtcatac ccatcaaata ggctgtgaga tggatcagaa aatcctgttt
    18601 ttgaatcctg gtagcatttc tcagcctaga ggcccgatcc aaatcccatc atatgctctt
    18661 atcgaaagtt tagatgacca aatttctgtt caatactata accgtgccca tcaggcagtt
    18721 gaagagttgg ccttttcttt ttcgagagag cgctgaataa aaaaactcta atttttaata
    18781 aaaataagct ttattttatg aattttgcat ccggtttcgg taaaatagta gtgttgaatg
    18841 aaatagaaat ggaggccatc agatgattgg acctatagta agagaaatgc tgctggaaaa
    18901 tcaggaaacg tttttgattc ctgcagaaaa tgtggcaaat gttatgtatc atcatccatt
    18961 gtcccatggt ttacttgtgt tatcgaaggt cggttattcc aaaattcctg tattaggaaa
    19021 agatgatcac tttgtaggac tagtaagttt atcagatgtg gtcaataaaa tgattgactt
    19081 gcaaacaatc agcatggacc cgttagaagg actaacggtt gctgatgtga cagaaacaga
    19141 tgttccgaca attgatgaaa attgggaact agaagaagta ctccatttgt tagtcgattc
    19201 gcctttttta ccggttgtca cagaagatcg gattttcaaa ggaatcatta caagaaaaga
    19261 gctgcttaaa gccgtaaact tcatggtgca tgaattagaa cgtcgtaata tattgtctcc
    19321 gaaaatcgaa gaattaaaag aacaaatcga tatcgtgagc taaagaacga gaaactaaaa
    19381 gataatcaaa aaataagtag aaaaaacagc gtaccagttt tgactttatc ttgaaaaaga
    19441 ggctgggaca aaagc
//
LOCUS       DACDXE010000060        19126 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.060, whole genome shotgun sequence.
ACCESSION   DACDXE010000060 DACDXE010000000
VERSION     DACDXE010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.060"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..703)
                     /locus_tag="IWM23_002065"
     CDS             complement(<1..703)
                     /locus_tag="IWM23_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884678.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="IWM23_002066"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="IWM23_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="IWM23_002067"
     CDS             1758..2837
                     /locus_tag="IWM23_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9884679.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="IWM23_002068"
     CDS             2869..3462
                     /locus_tag="IWM23_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884680.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="IWM23_002069"
     CDS             complement(3572..3799)
                     /locus_tag="IWM23_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9884681.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="IWM23_002070"
     CDS             complement(3902..6490)
                     /locus_tag="IWM23_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAP9884682.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="IWM23_002071"
     CDS             complement(6510..7169)
                     /locus_tag="IWM23_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9884683.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWM23_002072"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="IWM23_002072"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAP9884684.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="IWM23_002073"
     CDS             complement(7942..8808)
                     /locus_tag="IWM23_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9884685.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="IWM23_002074"
     CDS             8917..9558
                     /locus_tag="IWM23_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAP9884686.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWM23_002075"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="IWM23_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAP9884687.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="IWM23_002076"
     CDS             complement(10999..11898)
                     /locus_tag="IWM23_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9884688.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="IWM23_002077"
     CDS             complement(11900..12697)
                     /locus_tag="IWM23_002077"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAP9884689.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="IWM23_002078"
     CDS             complement(12675..13379)
                     /locus_tag="IWM23_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAP9884690.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="IWM23_002079"
     CDS             13510..14085
                     /locus_tag="IWM23_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAP9884691.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="IWM23_002080"
     CDS             14203..14880
                     /locus_tag="IWM23_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAP9884692.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWM23_002081"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="IWM23_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAP9884693.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="IWM23_002082"
     CDS             complement(16943..17941)
                     /locus_tag="IWM23_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAP9884694.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="IWM23_002083"
     CDS             complement(18167..18820)
                     /locus_tag="IWM23_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAP9884695.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4136 c         3324 g         6141 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagt
//
LOCUS       DACDXE010000061        18740 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.061, whole genome shotgun sequence.
ACCESSION   DACDXE010000061 DACDXE010000000
VERSION     DACDXE010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.061"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            256..531
                     /locus_tag="IWM23_002084"
     CDS             256..531
                     /locus_tag="IWM23_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884696.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            544..2616
                     /locus_tag="IWM23_002085"
     CDS             544..2616
                     /locus_tag="IWM23_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9884697.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2781..4667
                     /locus_tag="IWM23_002086"
     CDS             2781..4667
                     /locus_tag="IWM23_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9884698.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4679..5626
                     /locus_tag="IWM23_002087"
     CDS             4679..5626
                     /locus_tag="IWM23_002087"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9884699.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5646..6173
                     /locus_tag="IWM23_002088"
     CDS             5646..6173
                     /locus_tag="IWM23_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9884700.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6236..6889)
                     /locus_tag="IWM23_002089"
     CDS             complement(6236..6889)
                     /locus_tag="IWM23_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9884701.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7021..7863
                     /locus_tag="IWM23_002090"
     CDS             7021..7863
                     /locus_tag="IWM23_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9884702.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8021..8917
                     /locus_tag="IWM23_002091"
     CDS             8021..8917
                     /locus_tag="IWM23_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAP9884703.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8920..9633
                     /locus_tag="IWM23_002092"
     CDS             8920..9633
                     /locus_tag="IWM23_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAP9884704.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9653..10171
                     /gene="msrA"
                     /locus_tag="IWM23_002093"
     CDS             9653..10171
                     /gene="msrA"
                     /locus_tag="IWM23_002093"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAP9884705.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10168..10395
                     /locus_tag="IWM23_002094"
     CDS             10168..10395
                     /locus_tag="IWM23_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAP9884706.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10547..11098
                     /gene="lepB"
                     /locus_tag="IWM23_002095"
     CDS             10547..11098
                     /gene="lepB"
                     /locus_tag="IWM23_002095"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAP9884707.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11181..11723)
                     /locus_tag="IWM23_002096"
     CDS             complement(11181..11723)
                     /locus_tag="IWM23_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAP9884708.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
     gene            complement(11960..12358)
                     /locus_tag="IWM23_002097"
     CDS             complement(11960..12358)
                     /locus_tag="IWM23_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAP9884709.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12618..13478
                     /gene="ylqF"
                     /locus_tag="IWM23_002098"
     CDS             12618..13478
                     /gene="ylqF"
                     /locus_tag="IWM23_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAP9884710.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13471..14238
                     /locus_tag="IWM23_002099"
     CDS             13471..14238
                     /locus_tag="IWM23_002099"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAP9884711.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14294..15151
                     /gene="dprA"
                     /locus_tag="IWM23_002100"
     CDS             14294..15151
                     /gene="dprA"
                     /locus_tag="IWM23_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAP9884712.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15273..17351
                     /gene="topA"
                     /locus_tag="IWM23_002101"
     CDS             15273..17351
                     /gene="topA"
                     /locus_tag="IWM23_002101"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAP9884713.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17320..18696
                     /gene="trmFO"
                     /locus_tag="IWM23_002102"
     CDS             17320..18696
                     /gene="trmFO"
                     /locus_tag="IWM23_002102"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAP9884714.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5092 t
ORIGIN      
        1 ttttgtcgca gccccatttt tttgttgtga taaaagaaca agaaaggcag gaatgggaaa
       61 gtaaacatcc ttttgtcaga aacttttatc tttcttaagt tttacttgat atctgatact
      121 atttggaata gaatgaggcg ttgaaaaatt tatggaaatt tctaactagc aataaacgat
      181 gaaacaatga aatatttttc atccaaaaag aactttgcta taatgatgga agtttgtgta
      241 taggagatct ggaaaatgag agagagtttt gaacaacaaa aaaaactgtt gcatgatcgc
      301 tatggcgccc tttcgatgga tgaccgccgt caaatacttt gtaagttgcg taaaagaaat
      361 atattgatgt atcgtcagtt ggaacgtctg aaacatgatc tgcttcgttt ggagtcaaaa
      421 cgtgtacagt gtgaattaga ggggaaccaa acacaggttg aagtagtcga aaccaaaatt
      481 ttaaagaaaa aagaacaatt tttaaaaatg cttacacaaa ataaaaaata aaggggatgg
      541 acgatggaat ttgctgagtt agttattgta tttgcagtaa cgatcacctt atcgaacata
      601 gctagccgca ttttgccaat gattccagct ccactgatcc aaattttcct tggagtggta
      661 ttaggtctga cagagtgggg acaatcaatc gattttgaac ctgaattgtt cttagttatg
      721 attattgcac cattgttatt tagagaagga gaaaaagcag atatttcttc gattctcaaa
      781 aatttcggga caattttgtt cttggcattt ggaggagtca ttttgaccct tgtcggggta
      841 ggtgcgacgt tatcattcct gttgccaagt gtcccacttg ctgcctgctt tgcttttgga
      901 gcagcgttag gtccgacaga tgcggtagca gtcagttctc tttctggacg agtgaatatc
      961 cctaaaaagg cgatgcatat tttggaagga gaaggtcttt tgaatgatgc atctggtgtt
     1021 actgccttcc agtttgctct tggtgcgctg atcaccggat ctttttcagc agtaaacgcg
     1081 gggatgagtc tagtagtttc tagtattggg ggagctttga tcggcttttt gcttgtctgg
     1141 ttcaaacaaa aaataattca tttgatcgaa aaagcatcag cacaagatgt cacagggtat
     1201 ctgctgatcg aacttttgct tccttttcta gcctatgtat tggcagaggc atttgatgta
     1261 tctggaatca ttgctgcagt ggcagcaggt attctccaag cttctggttt taggaaaatc
     1321 tctgtgtttg atgcagaatt gtcgagcttg tcccacagta catggacaac tattgctttt
     1381 acactgaatg cattagtatt tatattttta ggaatcgaat tgacgcaagt gttctcgcca
     1441 gtatggggag acggtttata tccaaacggt cttttattag cgattatcgt attaatctct
     1501 gtcatgcttt ttgtgatcag attcatttct atcagtcttt tttatgtatt caaagatggc
     1561 agtaaaaagt tcaaaaaaca gttgaacgag atcttgatct taacatttgg cggtgtaaag
     1621 ggaacagtca gtttggctac gatttttatt ttgccatttt caatcaataa tatgatgttt
     1681 taccagcggt ctttgctttt gtttctgacg gcgggtgtca tcttagtgac tttggtcata
     1741 gggatcattg tcttgcctat gctgacggaa acagaggaag cgaaaagcac ggatttgaat
     1801 gctttgatga ttttagaaga ggtcgttgaa gttttgcgaa aagaaataaa ggaaattgat
     1861 cgtaatacaa aagaattttt agccactgaa gcagtgatag aaaattatca agaacggata
     1921 cgtgatctgt acttggaaga tttaaccgat gatgaaaagc aagaagtaca agaaatacaa
     1981 gcactgattt tgtcgatcga acgggatgga ttggatgaaa gttatcgttc gggcaagctt
     2041 tcttctaatg gttatcgttt ttactcccgt tttctttctc gattcgaaca ttctatcaca
     2101 agccagattt tgtcttttat cggcttttgg ttcattgttg tccgtaggtt aatgcgaatc
     2161 gttctccatc cgaaaatgtt ttggcaaaga agatatgctg atcgtcagac atttattagt
     2221 gatgaagata tccaagaaat cagagagaca tatcaaaaaa atactcagtt gattattgaa
     2281 agtttagata acttgactga tatttatgat gaaacgatgg ttcaattttt tatccagcaa
     2341 cgcaaacttg aaggaataaa aatgaactcg gggaatttga tttcttcgtg gatgatccag
     2401 caagatagtc tgtttacgaa aaaaatgcta agaggttatt atcttgaaag aaaaatcatt
     2461 gatgaatacg aagttgctga aaaaatcacg acgttttctg cgaacagcta tcgcaggaat
     2521 atcaatttgt tagaatctta tacgatgaac aaaccatctg ataacttttc tttccgattt
     2581 gcgtttcgag caaagtcaaa aaaacgagtc caataaaata aaaaggtaaa aattttcaag
     2641 ttttataaaa aacaaaacat atcagaacag gatcgaatcg gtcctgttct ttttttgaga
     2701 acacaaaaag aacaaatacc aaaaaaattt tcctgaagtt cactattttt atgatacaat
     2761 atccaagata aggagggaac atgttgaaag aattaaaagt aactgacctc aaaaaaactt
     2821 atggtgaaaa agatttgttt gaccagattt cttttttggt ccacgaaaaa gaccgtatcg
     2881 ggttgattgg gacgaacggg acgggtaaaa caagtctctt aaatattatt gccggtatcg
     2941 atagcagcga cggtgatcgg caaacagtgt tttatccaac tgattaccgt attggttatc
     3001 tttctcaagc agctgaattt tccgatgaat taactgttct ccaagcagtc tttcaaggaa
     3061 acagcccctt gatccaaact gttcgagcat atgaggaagc attgatcgaa ctaggggaaa
     3121 acggagaaga ccctgatgtg caaaaacgtt atgctaaagc agaagaacaa atgaataaag
     3181 aagatgcttg gactacagat accaacgcaa agattatttt gcaaaaatta ggaatcagtg
     3241 aattagataa aaaaatcagt actttatcag gtggtcagaa aaaacgagtt agtctggcgc
     3301 aagtgttgat tgatgaaccc gatttactat tactagacga accaacaaac catttagatt
     3361 atgaagcgat cgagtggttg gaaaactatt tgaaacaata ccgtggcgca ctattgatgg
     3421 ttacccacga tcgttatttt ctagatagag tagcaaaccg aatcttcgaa ctttcatttg
     3481 gaaaattata cgagtataaa ggaaattatg aagcttacgt cttagaaaaa gcagaacgtg
     3541 atcgtgtaga agtagaacaa gaagaaaagc gcaagcggct ttataaacag gaattagctt
     3601 ggatgagggc tggcgtacag gcacgtggca ccaagcagca agcacggatc aaccgtttcg
     3661 aagatctaaa ggagaacttg taccaagtaa accaagaaga tgatttagag ctgaacctag
     3721 cgacacaacg tttagggaaa aaagtactgg aaatcaaaga tggcagttac cgtataaatg
     3781 accaaacact gctagaacat ttagatttat tgattcaatc tcgggaaaga ctaggtatta
     3841 ctggtaaaaa tggtgcggga aaatcgacat tgcttaatat tctagctggc agaattcccc
     3901 tggatagcgg tacaatgtct atcggtgaaa cggttcatct tgcgtattat acacaagaaa
     3961 atgaagaaat ggcaccagat aaacggatga ttgcttactt acaagaagct gctgaagaag
     4021 caaaaacagc agatggcagt caaatcggag tagcagaatt acttgaacga tttttatttc
     4081 caagatttat gcatgggaca ctgataggca aactttcagg cggagaaaaa cgccggttat
     4141 ttttgttgaa actgctgatt cagcaaccta atgttttatt attggatgaa ccgaccaatg
     4201 atttggacat agcaacattg acgattttgg aagattattt tagaagtttt ccaggtgctg
     4261 tgatcactgt ttcccatgac cgttatttct tggacaaggt agcagataag ttattagtat
     4321 tccaaggaaa cggaaaacaa gaactttatt acggaaacat gtccagttat ttactgaagc
     4381 aaaaagagac ccagcagcct gctgaaaaag cgaaaccaaa aatacagagc aaggaacctg
     4441 ctgaaaagaa gaaattatct tacatggaac aaaaagaatg ggaaacgatt gaagatgaga
     4501 ttgcagaact tgaagaaaaa atctcattgc tccaagaaga gatgaatcat caaggagata
     4561 atttcactcg attacaagaa ttgcaaaacg acgtatctga aacagaagca caacttgaag
     4621 aaaaaatggc acggtgggaa tacttgagtg aatgggtaga agattaggag gaaaaaggat
     4681 ggaacaagcg tatcttgact taggaagaaa attgttggaa gaagggcatc gtaaagaaga
     4741 cagaacaggc acaggcacat taagcttatt cggataccaa atgcggtttg atctgcaaaa
     4801 agggtttcct ttgttaacaa cgaaacgagt accttttgga ttgatcaaaa gcgaattgct
     4861 ttggttttta aaaggagata cgaatatccg ctatctatta caaaataaaa accatatttg
     4921 ggatgaatgg gcatttgaac gctacatcaa aagtccagac tataatggac cagatatgac
     4981 tgattttggc catagatgtt tgacagatcc tgaattcaat gaagaatata aaaagcagag
     5041 caagcttttt tgcgagaaga tcctaaccga tgattccttt gctgaaacat acggcgatct
     5101 tggacacatc tacggctatc aatggcgtca ctgggaaaca aaagacggtg gctttatcga
     5161 tcaaatcaaa caagtgatcg aagcaatcaa aaagactcct gattctagaa gattgatcgt
     5221 ttctgcatgg aatccggaag atgttcctag catggctttg cctccttgtc atacgatgtt
     5281 tcaattttat gttcaagagg gacggctaag ctgccaattg tatcaacgca gtggggatgt
     5341 gtttctaggc gttccgttca acattgcaag ttatgcgttg ctgactcacc taattgcaca
     5401 tgaaacaggt ttagcggttg gcgaatttgt tcacacatta ggagatgctc atctttatat
     5461 caatcatatc gaacaaatga aagaacaatt gagccgagaa atacgcgcat ttccgacttt
     5521 agttttgaat caggaaaagc agtcagtatt tgattttgag atggaagata tccaattaga
     5581 aggatatgac ccacatccgg cgatcaaagc tcctatcgct gtttagaatc agaaaggatg
     5641 agaaaatgtt agctgcaatt tgggcacagg atgaaaacgg attgattgga aaagaagacc
     5701 gtcttccttg gcgtttgcca aatgatctga aattcttcaa acaaatgaca gaagcaaata
     5761 cactagtgat gggacgaaaa acattcgaag ggatgggtag ccgtccgttg ccgaatcgtc
     5821 agacgattgt tttgacgaga gattcttcct ataaagcaga aggcgtgcat gtcatgcata
     5881 gtgtggaaga cgtgcttgct ctggaaaaag aaacagacgg tatctttttc atcgctggag
     5941 ggtcagctgt ttatcaagaa tttctgccat tttgtacgat attataccgt acagtcatcc
     6001 atcatgtatt cgatggagat gcttattttc cacctgttga ctggtctgat tggtcactga
     6061 tcaatactag tcaaggagaa atggatgaaa aaaatcgtta tccgcatcaa tttgaaacgt
     6121 atcagcgtaa tgagaatcct gtaaaaagcg ggacaggcga attgacgata taaaaaagct
     6181 atggatagac tagctgttat ttagagctgt cccatccata gctttatttt ttttattaag
     6241 taaagaataa aacagagaag tacataaaac ctgcacctaa cataacaaac aaatgccaaa
     6301 caacatgcat atagcggata gaattcaagc tataaaagaa tgcgcctatc gtgtaagaaa
     6361 gcccgcctgc taccatcaaa ataacaccag tcattccaag agagtgataa agaggaacag
     6421 cagccacgac acagagccac cccataaaaa tatagatgat cgtagatact tttgatactc
     6481 tctcccgttt atgcaacgtc aacgctttat atataatgcc aagaatcgca aacaaccaga
     6541 ttaatacaaa taaagtccaa cctagccatc ctcggatact cagcaaacaa aaaggagtat
     6601 aacttcctgc aatcaaaaga aaaatcgaac tatgatcgaa tacttggaac accttttttg
     6661 cttttgtaaa aatcaatgaa tgaaacaaag tagacgttaa aaataaaaga atcatcatcg
     6721 atccatagat agcataagag acaatatgga gggaggaatg caagtgggca ccttttacta
     6781 aaagaaccac tagtcccgcg atactcaatc cagtcccaat tccatgggtg actgcattaa
     6841 acacttcatt taatatttca tacgttcgat taaatttcgt tttttccatg aaaacggctc
     6901 ctttactagg tgtaattaat gaaaaatcat tatttgtctg ttatactaaa aatagaaatt
     6961 gtcttacatg tagtttaaca tgaataaggc atcttgttaa agaaagagag agagaaaata
     7021 atgacaaatg taaaaatcgt aacagattct tcatgtacga tggaaaaaaa tgtacgcgat
     7081 gctttaggca ttcgtgtcat gccgctatcc atcatgatcg acggaacagt atatcccgat
     7141 gatgaccaac tagacggcga aagatttatg gaaatgatgg cgaaagcaaa aaatctgccc
     7201 aaaacaagcc aaccaccaat tggagaattc atctcgatgt atgatgagct aggagaagac
     7261 ggcagtgaag tgatttcgat ccatatgaca aaaggcttga gtggaacagt agaagcagca
     7321 agacaagcca gtaatctaac aaaaacgaaa gtaacggtta tcgacagtga taccactgac
     7381 caaggattat cttttcaggt aattcgagca gctgaattag caaaagaagg taaaacagta
     7441 gagaaaatcc ttgaagaagt caaaaaagta caagacaata caaaattata catcggaata
     7501 tccacactag ataacttggt aaaaggtgga agaatcagcc gaacaactgg tttgctgtct
     7561 agtgttttga acatacgcgt ggtgatgaat ttcgcccatt ctgaattaat ccctgtaact
     7621 aaggggcgag gaaagaaaac gttcgataaa tggtttaata atttgaaaaa tgaactgaaa
     7681 gaattgccaa atgtgcgaca aatcggtatt tcacatgcgg atgcaaaaga attggcgcaa
     7741 gaattcaaag agggtttgca agaattattc ccggacatgc atatccctgt actgcatacg
     7801 aatccggtta tcgctacgca tactggtaaa gacgcgtttg caatcatgta ttatgtagat
     7861 tagtcgaaaa gaggataagc cctcagcctg aaaggcttgc agcttattct ctttttcttt
     7921 ataaaattag gaaaataatt gttttctttt ttcataaaga ctaccttttt gataaaattt
     7981 ttgataacat tagaaccaat cgaatacaag aggtgaaaaa atgaaattaa cgaaacgaca
     8041 tttgatcctc gttttgttcc cattattaat tgcgatcatc atctatgcag gtttagctgc
     8101 ttctataccg aaagctggcc cactaatgaa accagtaact actgtgagag aagatccaaa
     8161 taaaaaagaa aaacttcact acatagctat cggagattcg ctgactgaag gagtaggaga
     8221 tcaaaccaag caaggaggat tcgttcccct tgtcgcaaat gatctaaaag accgttataa
     8281 tttgaccgcg gttgaaacag aaaactatgg tgtaaatggt gaacgaagtg accagatctt
     8341 aaagcgagta aaaacaaaag aagaaattcg aaacaatatc aaaacagctg attttatcac
     8401 actgactgtt ggcggtaatg atttgatgaa agttatccaa aataatttat tcgggttaac
     8461 ggtcaattcc tttaaaaagc cactgaaaaa ctatcaggaa aatgtcacag aactattgga
     8521 agaatttcga tccctcaata aaaccgcacc tatttacatt ttaggcatct acaatccatt
     8581 ttatttgaac tttcctgaga tcactgacat gcagacaatt gttaacaatt ggaacaaagg
     8641 aacagaaacg gtgacaaaaa aaatgccgaa ctgctacttt atcccaatca atgatttgct
     8701 ttatcaagga ttgaatcagg aagtaggtgt cacagaagaa ggaacagatc aaacaactag
     8761 cacgacagaa aatctaaatg gcatcaaaaa taatgtgctc tatgaagaag atcatttcca
     8821 tccaaataat ttaggttatc aattgatggc aaatgcagtg agagatcagc tgatcgagac
     8881 acaaaaccaa tggttagaga aggaggatga tcaataaaca tgaaaacaag atctgacgta
     8941 tcttcaaaca aaacaaagaa aacaaattac tggaaattcg ccttccttat ccttcttggg
     9001 gtagtacttg gttcgaccgt atttttaggc agccgtattt ttgctaatcg ggaaccagaa
     9061 ctcccagaaa taccagctct tacggaaaga caaggagatc ctgtcttaac gatcaattca
     9121 aataaagaaa aagtcaatca aatcatcagc tttttcctta gtgagtatca aaaagatagt
     9181 gatattgaat ataaatttta tttggaaaat gaagcactac tcaatggtac tttcgaagta
     9241 ctaggttttc ctatcgattt ctatttatat tttgatccgt atgtgatgga aaacggaaat
     9301 gtccaactaa aagctagaag tctttctatc ggaacattga gtttgccaat caaagatgtg
     9361 atgaatatga tcaagagaaa ctataaatta ccagattgga tcgaagtcaa tacagatgat
     9421 ctgactatca tgcttaggtt ggatcaattc cgaatgcaaa acgggatgta tattaaagca
     9481 gataaaatcg acttagtaaa tgatgacatt cggtttagtc tatatttgcc aaaagatacc
     9541 tctgatgctt caaacaactc aaataccaac aaaactagca ccagcagtac tgaaagcaag
     9601 acaaaagaaa ataaaaaatc atcggatgag tagtattaga gggggaatca taatggaacg
     9661 agcaatattt gcaggaggct gcttctggtg tatgatccag cctttcgaca cgcttcctgg
     9721 tatccataca gtgatgtcag gatatactgg agggcatgta ccagatccga cttatgaaca
     9781 agtaaaaagc caaacgactg gacatacaga agcagtggag attatctatg atccaacatt
     9841 gatttcatat gaacaattag tagaacttta ctggcagcaa actgatccaa ctgacgcttt
     9901 cggacaattc gaggatcgcg gtgacaatta tcgcccggtg atcttttatt tgacagaaga
     9961 acagcggatc attgctgaaa aaagtaagaa acagttagct gaaagtggac gtttcgaaca
    10021 gccaatcgtt acaaaaatcg aaccagccca accattttat ctagcagaag aagaccatca
    10081 agagtattat aaaaagcatc ctgaaaattt taaaagaaac catgcacgcc gggctgcctt
    10141 tatcgaagaa aattggagga acgaagggtg aatcgaagct tttttcatta tttaatgaca
    10201 ctgcgaggcg gtggcacaca tgatgcttta tctgttttcg ctacagaagc cggtaaagac
    10261 atccaatttc ctaagcacag ctctagctac gaagaaattt ctaattatct ggaactgaat
    10321 gtcgactatt tgccgtccat ggatatcttt gacgaagcat gggaaaaata tttagaaaat
    10381 aatcatgaca tataagagat agttttgtac tttatgatca aaaaagtttt ttgtccaata
    10441 gcctaattga aagaattatt tttatttcag ctatctagtc gcattttttt gatatttttc
    10501 ttataataaa gaaagaatga aaaaaagagg gatggattga tagagattga caaaaaaaca
    10561 acgctatatt aatcgcttct ggcttgtatt caaatactta cttgtttctg tattccttgc
    10621 gttcatgttg cggggatttt tattcatccc ggtaccagtg gaaggcaatt caatggaaaa
    10681 cgtgttgaag caaggtgata tggttgtgat ggaaaaattt tcggagatca gacgatttga
    10741 tattgttgtt tttcaattag cagatggcac gatttatatc aaacgagtaa tcggtcttcc
    10801 aggagaaaat gtcagctacc aaaatgatca attgaagatc aatgggaaag tagttaagga
    10861 accttatcta actaaaaacc tgaaatcaga tcatgcaaat gcatcctata cgacggactt
    10921 tacactacaa gagctaacgg gacaatccaa gcttccggaa gataattatt ttgtacttgg
    10981 agataatcga agagtttcaa aagatagtcg ttcgtttggc acgatcaata aaactgatat
    11041 tctaggtaaa gcaagatttg tctattatcc acttgatgaa ataaaatgga tccagtgaga
    11101 aacaataaag cgtgcagtct gttgagactg cacgctttat tgtttaatcc aaaatcagtt
    11161 tactacctta gtacgtaaaa tcaaacagct cgtttaaaca cttttctagc ctctaaagtt
    11221 ttgataaatg aataaacaac cattatttga atagggattt ggatcatatt cctgatgaat
    11281 ctacctagaa aaattacttt gaaatctgca ccaaacaaaa cagacatcag ccaagaattg
    11341 ataaaaaggc tgactgggat cataatcaat aattgggcag cgatgacgga aggcaggttt
    11401 atctttttat gatataaaaa taagccatta ataaatccac caaggaaagc cgtgataaga
    11461 aaagcagctt gaaacgttcc tgaaccaaat atcgtgaaat ttaaagtgtt cgacaacatt
    11521 tcaagcaaac cagcccagat cggtccatat agataaccca taagcgccat acttaaaaat
    11581 ccgaatgtga tttgaaaata agcaggacca atcgctatct tgtataagat gacatcaagg
    11641 gctaataaaa gagcagaggt cgacaacaat cttgcattca tttcgggcaa tctaaaaacg
    11701 tcttttgcaa tcaaagtgtt caaaatattc tctcctttgt cagttaataa taaaaggata
    11761 ccataaaatc accaatgtgt catgacacat tggtgattta catacactgg catctaacaa
    11821 ttaaaaaaag ggtgagaaga ctgcttttat aaactgtaaa aaagagattg tacgaataaa
    11881 aaaaatagag gcggagaata cttatcgtat catccgcctt tgtttttttg ttataaaaaa
    11941 cgtcagatta gttatctgct tacatcttca tccatacggg ttctaatgta tttccgtcta
    12001 gatcttggac ttctaatgaa aacatttctt cttcaggcat tcccatatct acttcgtgga
    12061 aagtaccacc attttctttt gcaatttgag caaatttttt tacttcatcc gcgttttcaa
    12121 cactaaacga aatcaaagca ccacttgttt ttttattatc agctatttct ttatctttta
    12181 taaatttttt ataaaaatca tgagagagaa gcattatcca aaaagtgtca tcccatacca
    12241 ttgaaccagc ttcttcagta gaaaattctt catttttttt aaaacccagc ttttcataaa
    12301 aagcaacgga acgattgaca tcagcaacag gaaaattaac aaaaaccata ctattcattt
    12361 gtttcgtctc ctttctgttt tgttcgtcta tcatctctta tttaggaaac gctgtcaaaa
    12421 atttagattc atatatagaa accaaaataa aaatttgcat catcaaaaaa gtgaagatgt
    12481 gatagaatga ggactggttc actttcactt aactaataga tggaatttct ttataataaa
    12541 aaagtgaatt tatattgaaa aagaaaaaaa caagtaaagc cggggaatat tccggtatga
    12601 aaggaatgga gagaacaatg accatccaat ggtttccggg acatatggca aaagcccgac
    12661 gagaagtttc tgaaaaaatg aaatatgtgg acatcgtatt tgaattggta gacgcacgat
    12721 tacctttatc ttctcgtaat ccaatgctag atcagatcat tcagcaaaaa cctcgccttg
    12781 ttttattgaa taaagcggat ctggcagatc cacaacagac aaagctgtgg caagaatatt
    12841 ttcgtaaaca aggccatctt gcattagcta tcacggcaca agaaagtaaa ggaattaaag
    12901 gtctagtacc tgcggctaaa gaagcattga aagaaaaacg ggcaagagat gctgctcggg
    12961 ggatcaagcc tcgcgctatt cgggcaatgg tcttaggtat tccaaatgtt gggaaatcta
    13021 cgttgatgaa tcgactagta ggcaagaaaa ttgcacagac aggaaataag cctggcgtaa
    13081 caaaaggtca gcagtggctt cgtttaggta atgagttgga attgttagac acaccaggga
    13141 ttttatggcc caaatttgaa gatgaagaaa tcggcaaaaa actagcgctg acaggcgcga
    13201 tcaaagacca gcttgtccat ttagatgatt tagcgattta cggattagac ttttttgccc
    13261 gatattattc aggaagaatc agcgatcgtt atggtttatc aatcgagcaa gaagaaaaat
    13321 tggcaccgga attactgatg gatattacag caaaacgtgg gtataaagaa gactatgatc
    13381 gtggcagcga attagtcgtg catgatattc gtcaaggaaa acttggacga tacacactgg
    13441 atcgttgcga agaaatggag caaaaaagtg atgactgaat ccatccaaag tatcaaagaa
    13501 cgattgaaaa gcatacagag aatagatgat cctacactag aatcattaag aaatgatcca
    13561 cgcaaaggcg tgcagcaagc actggcttct tttgaacgac gattgaaaaa ggaagcatta
    13621 attaaggaaa aatatgaaga aatgttttgc tttgaaaagc aagggtacgc acaaggtcac
    13681 caatggattg ccggcattga tgaagtagga agaggaccat tagctggacc tgtcgtggca
    13741 gctgcagtta tcttgccgaa aaatgagatc atattagggc tgaatgactc caaacaatta
    13801 tcagagaaaa agcgagaact tctttatcaa caagttcaag aaaaagccct ggcaatcggg
    13861 atcgggatcg ttgatcagca gacaatcgat gatgtgaata tttatgaagc aagtaaatta
    13921 gcgatgatcc aagcagtaga aaaattgtcc attgtaccaa gctatttgtt gatcgatgcg
    13981 atgaccttgg atttgcctat tcagcaggaa aaaataatca aaggggatgc acgatctgtg
    14041 tcgattgctg ctgcaagtat cattgctaaa gtatatcgag accaattgat gaaagaatat
    14101 gacgtgcttt atcctggata tggtttctcg cataatgcag gttatggaac aaaggaacac
    14161 ttagaaggac ttgaacgaca aggaattacg ccaatccatc gtaaaagttt tgcacctgta
    14221 aaaaaatatt tacaataaaa aacagccgct tttacgtatc ttattagaaa gagagacgaa
    14281 aggcggtttt atgatgtatc aaatagaaga aaatttattg aaacttgctt atacaaaagg
    14341 aatcagtagt caaggaaaat gggcacttgc taactggatg atgcagtatc cttataaaga
    14401 aatcggattt gaagacatca ttaagatagg taaaataacc aaacatcggg agctgtttcg
    14461 caaaagttgg caggaaattc acgctaactg gggcaaaatc caatcgaaac agtcgtttat
    14521 cacttgcttt gatcccaaat atccccctca gctgcttcac ttgacctatc cgccaattgt
    14581 actgttttat aatggtgatc tatctttact ttcttgtaac atgttaagcg ttgttggtgg
    14641 aagacacact tctgctttag ccaaaaaaac agtttattat ttactaaaac cagtgataga
    14701 agctggatat gtgatcgtca gtggctgcgc gaaagggata gatacatacg gtcaccaagc
    14761 agcaatctct catggtggac ggacaatcgc agtcattgga acagggatag agcaagtcta
    14821 tcctgctgaa aatcgtgact tgcaagaggt gattgcaaat gatcatcttc ttttgagtga
    14881 atatcctcct caatccggcc cgaaaaagca ttgttttcca atgagaaatc ggattatcgc
    14941 agctttgtct aaaggaacat gtgtctttga agccaagaaa aaaagcggct ctctgataac
    15001 agcacagcaa gcattggatc ttggaagaac agttttttct gttccaggaa atattttaac
    15061 agaacattct accggatgtc atcatttgat ccaagacggt gcagtttgca cggtttcggg
    15121 tgaagatatc ttggcagaat ttaaagaata acaccatact tttcatttgc ttttcttgac
    15181 aaacgttttc tgaatggata tgattagcta cgatttgtat gaatcctctt ttaatagtag
    15241 gaacacgaca atggaaagga gtccattcag taatggcgta taaatattta gtgattgtag
    15301 aatcacctgc aaaagccaag acaattgaga aatatcttgg acgaaactat aaagtaatgg
    15361 ccagtgttgg ccatataaga gatttaccga aaagtaaaat gggtatcgat tttgaaaaca
    15421 attatgaacc ccattatatt tctatacgcg gaaaaggcga tgtcatcaaa agcctgaaag
    15481 ccgcagcaaa aaaagctcaa aaagtttact tggcaagtga cccggataga gaaggagaag
    15541 cgattgcttg gcatttagcg tacctacttg ggttggatct gaaagaaaaa aatcgggtgg
    15601 tcttcaatga aatcacaaaa gacgcagtca aagcagcttt taaggaacca agaacgatcg
    15661 atgtagattt agtagatgca cagcaagctc gtcgtacctt agacagaatc gttggttatt
    15721 cgatcagtcc tattctctgg cgtaaggtca agaaagggtt aagtgcagga cgtgtccaat
    15781 ctgtcgcttt aaaaattatt attgaccgtg aaaaagagat ccgagaattt gttccagaag
    15841 aatattggag catcgacggt aattttaaaa aagctcgcaa gaaattcaaa gcaaatttct
    15901 ggggaatcga cggtaagaaa aagaaattac cagatgcaca aagtgtaaaa gaagtcactg
    15961 ctagaattga tggaacagac ttcgaagtta aaaaagtaga aaagaaagag cgcaaaagaa
    16021 accctgcgaa tcctttcact acaagtagcc tgcaacaaga agctgcccgt aagttaaact
    16081 ttcgaacaag aaaaaccatg atggtagcac agcaattata tgaaggaatc actttgggta
    16141 aacaaggaac agtcggcttg attacataca tgcgtactga ttctacccgg attgccgata
    16201 cagcaaaagc agaggccgca gcatttattg aagatacgta tggaaaagaa ttttctagac
    16261 atgatcaccg aaagatcaaa aatgctcaag gcgcacaaga tgcccatgaa gctgtgcgtc
    16321 caacgagtgt gcttcgtaca ccagatgaac tcaaacaata tttagataaa gatcaattga
    16381 aactttatac attgatctgg tcacgttttg tggctagtca gatgacacca gctattttag
    16441 atacaatgaa agtgacatta gaacaaaatg gtgtcatctt cattgcgaac ggttcaaaaa
    16501 tcaaattcaa aggattcatg caagtgtatg tggaaggtag agatgacggc aaagaagaga
    16561 aagaaaatat tcttccagaa cttgaagaag gcgatgtagt ccaatctgtt gatattgaac
    16621 caaaacaaca ttttactcaa ccaccagcac gttttagtga ggctacgttg attcgtgcac
    16681 tagaagaaaa cggggtaggg cgaccttcca cttatgcacc aacacttgat acgatccaaa
    16741 gacgctacta tgtaaaattg acacaaaaaa gatttgagcc gactgaacta ggagaaattg
    16801 tcaattcatt aatctgtgaa ttcttccctc aaatcgtcga tatccatttc acagcagaaa
    16861 tggaaggaga tttggataaa attgaagaag gcacggaagc gtgggtcaaa gtggttgatc
    16921 gtttctataa accgtttgaa aaagaactga cgaatgcgga agaaaaaatc gaaaaaattc
    16981 aaatcaaaga tgaaccagca ggattcgatt gtgatgtctg tggacatccg atggtgataa
    17041 aattagggaa atatgggaag ttttatgctt gcagtaattt tcctgattgt cggaatacga
    17101 aaccaatcgt aaaagaaatc ggtgtgactt gcccagtatg tcatgaagga caagtcattg
    17161 aacgaaaatc taagaaaaac agactattct atggttgttc ccgtttccca gaatgtgaat
    17221 tcacttcttg ggataaacca gtgggcagaa attgtccaaa atgtgacgga tatctggtcg
    17281 agaagaaagt caaaggcggt aaacaagtag tctgtgtaaa tggagattac gaagaagaca
    17341 tccaaaaata atagaaaaga ggttctttca ttgaaaatgg taaatgttgt tggagcaggg
    17401 cttgccggaa gtgaagctgc ttggcagatc gcacaagcag gagtccctgt ccgtttatat
    17461 gaaatgcgtc cagtcaaatc gactgaggcg catcacacaa gtaattttgc tgaactagta
    17521 tgttccaatt cattaagagg aaacagtttg gctaatgcag tagggttatt gaaagaagaa
    17581 atgcgccgat tgaattcggt agtaatcact tcggcagatg aaacagcagt gccagctggt
    17641 ggtgccttag ctgttgaccg cgattctttt tctgaaacaa ttacccgaaa agtcaaagag
    17701 catccattag taacagtgat aaacgaagaa attactgaaa taccggaagg aatcaccgta
    17761 gtagcaacag gacctttgac ttctcatcca ttagcagaaa gcatcaaagc attcaatggt
    17821 tcagatggtt tttatttcta tgatgcagct gcaccaatcg tagacaaagc tacgatcgat
    17881 atggataaag tatatttgaa gtcccgctat gacaaaggag aagcagctta cttgaactgt
    17941 ccgatgacag aagaggaatt caaagctttc catgaggcac taatcaatgc cgaagtggca
    18001 gaacttaaat cttttgagaa agaaaaatat tttgaaggat gtatgccaat tgaagtaatg
    18061 gcacagcgag gattcaaaac gatgcttttc ggacctatga aaccagttgg attggaagat
    18121 cctaaaactg gcaaacgtcc ttatgctgtt atccagctgc gtcaagacaa tgctgcagct
    18181 tctttataca acatcgtggg ctttcagact cacttgaaat ggggcgagca aaaacgtgtt
    18241 ttccggatga ttcctgggtt agaaaatgct gaattcgttc gctatggtgt gatgcaccga
    18301 aacagcttta tgaattcgcc agaattgctg aaaccgactt accaatcaac gaaacgcgaa
    18361 gatctgttct ttgcaggtca gatgactggt gtagaaggct atgttgaaag tgcagcgagc
    18421 ggactacttg ctggaatcaa cgccgcacgc ttagcaaaag aaatggaacc agtcgagttt
    18481 ccacaagaaa cagctattgg aagtatggct tactacatta ctcatgcgga aggaaaacat
    18541 ttccaaccga tgaatgccaa ctatggtctg tttcctgaac tgccagagcg cattcgagac
    18601 aaaaaagcaa gatatgaagc aattgcacaa cgagctttaa aagctaatga ggcaataaaa
    18661 acagaattag aaaaaactgc aatcccaaat gagtaaggaa actccatgaa gagggtgaga
    18721 cggatagtct cgctcttttt
//
LOCUS       DACDXE010000062        18054 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.062, whole genome shotgun sequence.
ACCESSION   DACDXE010000062 DACDXE010000000
VERSION     DACDXE010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18054)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18054)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18054
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.062"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            214..498
                     /locus_tag="IWM23_002103"
     CDS             214..498
                     /locus_tag="IWM23_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAP9884715.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="IWM23_002104"
     CDS             complement(779..1198)
                     /locus_tag="IWM23_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAP9884716.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="IWM23_002105"
     CDS             complement(1200..1784)
                     /locus_tag="IWM23_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAP9884717.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="IWM23_002106"
     CDS             complement(1831..3306)
                     /locus_tag="IWM23_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAP9884718.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="IWM23_002107"
     CDS             3487..3798
                     /locus_tag="IWM23_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884719.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="IWM23_002108"
     CDS             complement(3913..6249)
                     /locus_tag="IWM23_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9884720.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="IWM23_002109"
     CDS             6391..7059
                     /locus_tag="IWM23_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884721.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="IWM23_002110"
     CDS             7062..8105
                     /locus_tag="IWM23_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9884722.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="IWM23_002111"
     CDS             complement(8342..9223)
                     /locus_tag="IWM23_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9884723.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="IWM23_002112"
     CDS             9382..10110
                     /locus_tag="IWM23_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAP9884724.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="IWM23_002113"
     CDS             10266..10583
                     /locus_tag="IWM23_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9884725.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="IWM23_002114"
     CDS             10576..11184
                     /locus_tag="IWM23_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAP9884726.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11200..12030
                     /locus_tag="IWM23_002115"
     CDS             11200..12030
                     /locus_tag="IWM23_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAP9884727.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            12168..12680
                     /locus_tag="IWM23_002116"
     CDS             12168..12680
                     /locus_tag="IWM23_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAP9884728.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="IWM23_002117"
     CDS             12706..13578
                     /locus_tag="IWM23_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAP9884729.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="IWM23_002118"
     CDS             13754..14116
                     /locus_tag="IWM23_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9884730.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="IWM23_002119"
     CDS             14125..14877
                     /locus_tag="IWM23_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884731.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVKPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="IWM23_002120"
     CDS             14891..16693
                     /locus_tag="IWM23_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884732.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAEKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="IWM23_002121"
     CDS             16970..18010
                     /locus_tag="IWM23_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9884733.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
BASE COUNT         5663 a         3139 c         3729 g         5523 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gttttttttc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaactt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 tttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tttgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgtta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa ggttgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgactagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg taatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aagacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aagcggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgcat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg catgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtt ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaaatc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagact tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcaaacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttctggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cctggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctag gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgata ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtatagga gtagccttag ttacgatcgt
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctga aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcctatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagacat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggtatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt ttta
//
LOCUS       DACDXE010000063        17963 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.063, whole genome shotgun sequence.
ACCESSION   DACDXE010000063 DACDXE010000000
VERSION     DACDXE010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17963
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.063"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(83..751)
                     /locus_tag="IWM23_002122"
     CDS             complement(83..751)
                     /locus_tag="IWM23_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9884734.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            885..1172
                     /locus_tag="IWM23_002123"
     CDS             885..1172
                     /locus_tag="IWM23_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884735.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1588..3126
                     /locus_tag="IWM23_002124"
     CDS             1588..3126
                     /locus_tag="IWM23_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAP9884736.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3123..4088
                     /locus_tag="IWM23_002125"
     CDS             3123..4088
                     /locus_tag="IWM23_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884737.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4075..5397
                     /locus_tag="IWM23_002126"
     CDS             4075..5397
                     /locus_tag="IWM23_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9884738.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5460..5564
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5638..6381
                     /locus_tag="IWM23_002127"
     CDS             5638..6381
                     /locus_tag="IWM23_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884739.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6396..7388
                     /locus_tag="IWM23_002128"
     CDS             6396..7388
                     /locus_tag="IWM23_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9884740.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7400..8137
                     /locus_tag="IWM23_002129"
     CDS             7400..8137
                     /locus_tag="IWM23_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884741.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8127..8849
                     /locus_tag="IWM23_002130"
     CDS             8127..8849
                     /locus_tag="IWM23_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAP9884742.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8933..9556)
                     /locus_tag="IWM23_002131"
     CDS             complement(8933..9556)
                     /locus_tag="IWM23_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9884743.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            9891..10325
                     /locus_tag="IWM23_002132"
     CDS             9891..10325
                     /locus_tag="IWM23_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9884744.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            10348..10668
                     /locus_tag="IWM23_002133"
     CDS             10348..10668
                     /locus_tag="IWM23_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884745.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            10809..11000
                     /locus_tag="IWM23_002134"
     CDS             10809..11000
                     /locus_tag="IWM23_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884746.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(11071..11328)
                     /locus_tag="IWM23_002135"
     CDS             complement(11071..11328)
                     /locus_tag="IWM23_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAP9884747.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            11486..11749
                     /locus_tag="IWM23_002136"
     CDS             11486..11749
                     /locus_tag="IWM23_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884748.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            11773..11961
                     /locus_tag="IWM23_002137"
     CDS             11773..11961
                     /locus_tag="IWM23_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884749.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(12044..12229)
                     /locus_tag="IWM23_002138"
     CDS             complement(12044..12229)
                     /locus_tag="IWM23_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884750.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            12444..12815
                     /locus_tag="IWM23_002139"
     CDS             12444..12815
                     /locus_tag="IWM23_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAP9884751.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(12965..13037)
                     /locus_tag="IWM23_002140"
     tRNA            complement(12965..13037)
                     /locus_tag="IWM23_002140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13002..13004),aa:Gln,seq:ctg)
     gene            complement(13083..13511)
                     /locus_tag="IWM23_002141"
     CDS             complement(13083..13511)
                     /locus_tag="IWM23_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884752.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(13571..14329)
                     /locus_tag="IWM23_002142"
     CDS             complement(13571..14329)
                     /locus_tag="IWM23_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAP9884753.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            14624..14821
                     /locus_tag="IWM23_002143"
     CDS             14624..14821
                     /locus_tag="IWM23_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884754.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            14927..15409
                     /locus_tag="IWM23_002144"
     CDS             14927..15409
                     /locus_tag="IWM23_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884755.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(15467..16063)
                     /locus_tag="IWM23_002145"
     CDS             complement(15467..16063)
                     /locus_tag="IWM23_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAP9884756.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            16450..17916
                     /locus_tag="IWM23_002146"
     CDS             16450..17916
                     /locus_tag="IWM23_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAP9884757.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT         5889 a         2971 c         3670 g         5433 t
ORIGIN      
        1 aaagaagatt ttactgaata atatcatttt agtttacata atgatatttc tgtaaattaa
       61 ataaagtagt gaaaaataag aatcagtgct ctaactgttc aagaggatca agtggttcaa
      121 atgtttctgt agtctgtttt gcttccaatt ggggcaaaac agatccaatt tccagatact
      181 tcactttatt caatgtataa gatagaatag aacagctaag taatgtatac agaaaatgtg
      241 tgagatccaa ataaagcaag gagacaacta aaataccaaa agtaatcaag aaaagacttt
      301 tgccaatctt aatatgaaaa tatttttcaa tggcttgata aatgatatct gttccgcctg
      361 tgcttccgcc gaatcgaaag acgagaccgc aaccaatgcc agaaagaata cctgtcatca
      421 aactattgat cagtagacta tctacagcaa aatgtatatg aaggctctcc cataaatgta
      481 aaaagaaaac tagcgcaaat gttccgacga tcgtccggag aaaagcgtgt tttccaaaaa
      541 gaaacaatga gataagcaat aaaggtaaat taaaaagcag tgaagtgaca gaaggagcga
      601 atccaaaaag attatgcaaa agcaaagcaa taccagtaac cccaccatct gccagcttag
      661 atggaatatt gattcctgtc acagctaatc cgtagattcc tgcgcctaaa atgattccgc
      721 acaattctgt aaataaattt aattttttca tttctagcct cttttctttt gaaaattttc
      781 agcaacttct ttattatact gacttatact cttacttcaa gaggaaaatg aaaaaaaatt
      841 atttttggct gtttcttttt atttatcaaa taaggagtgg aaaaatgcga gcagcagact
      901 tattgacaat tcttacaaaa tcacccaaaa aaacagagta tcaaggagtt tttttggctc
      961 aagaacaaaa actggtgcct gtgacaatgg tccagataaa taaagcaaat caatttatct
     1021 ttttatttga acacggcaaa gcaccacttg ctatgaaaga catctttctt tttttgatga
     1081 agaaccgtga gaaagctgtt tattatcagt caaaagaaga actcattcct ctttatggag
     1141 tgaaagagat agaaaataaa gtggtgatat aagtaaaaat aagaaaacaa gacgtttatg
     1201 atgtcaaaac aaccattttt tacatttttg gcaaaaaagt gtcatgatac agtataatat
     1261 tcgttgaaca ggccaataaa ggtgttctcc cccacgagat ttctacttta ttggctgttt
     1321 ctttttatta aaataaaagt tgtgtgtgta aaaaagatat agctgattcc aagtgacaat
     1381 ttcgtataga tttttatggt attcgtagtg aaaaaacgag aaccaaaaag ttgatccgac
     1441 tttgttgctt ggattctttt tttgtcagca tatttagagg tatttttttc ttttttttgt
     1501 tcgtgctgta aggttaaggc aagacaagtt agttgggaaa aaggaaagaa atgtttatac
     1561 ttaaagaata acagaaagga aggtcgcatg aataatttta tacaaacatt aagtgaaaga
     1621 aaaggcgagt tgctggaagc tgcatttcaa catctgtcca tttctctcat ttctttgctg
     1681 attgcagcac tgattgctat tcctttagcg atttgggcag ccaatcataa aaaaatggca
     1741 gaagtcttat tgcagatcac gggtgtgctc caaacgattc cttcacttgc cttattaggt
     1801 ttacttatcc catttgtagg gatcggaact gttccagcat tgattgcact tgttatttac
     1861 gctttgcttc cgattttcca aaacacgtat attggtctag ctgaaattga cccgtctatt
     1921 gaagaagcag cggtagcatt tggtatgtcc agaatgagac gcttgctgaa agtagagctt
     1981 cccatcgcat tacctgtcat tatctcaggt atccgaactg ccttagtttt aatcatcggt
     2041 acggctactt tggcagcatt gatcggagca ggtggattag ggacatttat tttgttaggt
     2101 attgaccgaa atacacctgt tttgacattg attggtgcga tcagttctgc cttgcttgca
     2161 attatattca gcggactgat tcgctggttg cagcataaga aacctcgata ttccatcatt
     2221 actttagcag cagtggtgtg tttatttggc ggcgtttctt tatatcagtc tgccttgttt
     2281 aaaaatgaaa ccatcacgat tgctggaaaa ttaggagcgg aacctgaaat cctgatccaa
     2341 atgtataaag aattgattga agacaacaca aaaacagaag ttgaattaaa accgaatttt
     2401 ggcaagacga gttttttgtt caatgcactg gatcaaggtc aaatcgatat ctatcctgag
     2461 ttcacaggta ctgtcctaga aagcttggtg aaggtacctg aagcaatgaa aagtgctgat
     2521 ttgacaaaag aaaaaacata tgacgaagca gaagaattat tgaataagca attcgatatg
     2581 acattattaa aaccaatggc ttaccaaaat acgtatgcct tggcagtcaa gagatctttt
     2641 gcgaacgaac atcaacttaa aaagatttcc gatttggcag ctatctctga tcagatcaaa
     2701 gcgggattca cactggaatt tattgatcgt tcagatggat ataaaggaat ccaagaagca
     2761 tatggcttga atttttcaag tgtacaaagc atggagccaa gtttgcgtta ccaagcaatc
     2821 aataatggag atgtcaatat tgtggatgcc tattccacag atagccagct caaggagtac
     2881 gatctagtga ctctagaaga tgatcgaggg ttattcccag cttatcaagg cgcaccgctg
     2941 atgaaaaaag attttgctaa agaacatcct gaaatcgtta aagcactgaa taaattaagc
     3001 ggaaaaataa cggaggatca aatgattgat atgaattacc aagtcaatgt cgaaaagaaa
     3061 aagccgacag aagtagcacg aatcttcctg aaaaatgaag gattagtaaa ggaggaagat
     3121 cagtgactaa attaatcgag ttcaaacatg tgcaaaaaaa gtatgatgga aagtatgtca
     3181 tcgatgacct aaatctggca atcaaaaaag gagaaatctt tgttttggtt ggtccttccg
     3241 gtagtggaaa aacaactact ttgaaaatga tcaatggatt atctaaacca tcagcaggag
     3301 acatctactt caaaggaaaa tcgttaaatg aatataatct gcagaaaatg cggtggaata
     3361 tgggatatgt gctgcagcaa atcgctttgt tcccgaccat gacagtcaag caaaatattg
     3421 aagtcatccc tgaaatgtta ggttgggaaa agcagaaaag agcagaccgt gtagatgaac
     3481 ttcttcaaaa agttggctta tctcctgata tttatcgaga tcgtatgcct agagagttgt
     3541 ctggaggaga acagcaacgg atcgggatca tccgagctat tgctgcaagc ccagatgtga
     3601 ttttgatgga cgagccattt agcgcattag atccgatttc aagaaactca ttacaagaat
     3661 tggttttgtc cttacacgaa gaattgggca cgaccatcgt tttcgttacg cacaatatgg
     3721 aagaagcaat caagctaggt gatcgtattg catttatgaa ggatggagaa attatccaat
     3781 gtgacacacc agagcagtta ttgatgaatc ctaaaaacga ctacgttcgg catttctttg
     3841 atgaaccgaa acagacgaaa gagtggcgag tagaagattt agtcgtcaat ggatatttct
     3901 taaatgaaat accagaaaat gctcgtcaac aagtttgctt tgatacgcct atgaaagaag
     3961 tatattcgct tctttcggtg tatccaagca ttgtgatagt agaaaagcaa agaagtattg
     4021 ggtcgctgac aagtaaagaa atctttgctt ttttaagtcg agaggaggaa ggaaatgaaa
     4081 acatatgatg caatcatcat cggttcaggt gtgagcggct taagtgctgc ttatggattg
     4141 aaagaagcag gtaaaacagt actagtggtc gaagaagatc tatggggcgg gacttgtcct
     4201 aaccgtggat gtgatcctaa aaaagtcttg ttaagtgcag tagaggcacg taatcgggta
     4261 aaacaattga gtggaaaagg ttttaacgag atcccaactg ccaactggga agaacttcaa
     4321 aaattcaaac gaacatttac ggatccagtc ccagaaagcc gaaaaaaaca attagcagaa
     4381 gctgaaatcg atcatctttc aggaaccgct cgttttttgg atgattcatc tattgaagtc
     4441 aacgaagaag tcttccatgc ggattactta gttttggcaa ccgggcaaag acctacgatc
     4501 ttaccagtgg agggaaagga atacttgaaa actagtgcag actttttatc attgcctgta
     4561 ctgcctaaag aaatcatttt tatcggtggt ggttatattg cttttgagct agctacgatt
     4621 gccaatgctg cagggagcaa agtaaccatt gtccatcata atcaacggcc attaaaagaa
     4681 tttgaggcgt ctttggtaga ggaagccgtc catcaaatgg aagcatctgg cattcagttt
     4741 gcttttggtg ttgaaaccca aaagatcatt tcagaaggta ctcgctatcg tttagtcgga
     4801 aaagagacgg aattagtagc agatatgatt ttttgtgcga ctgggagaca gccgaataca
     4861 gaatctttag cacttgagca agcgaatatc gtctttgata aacacggaat tgcagtcaat
     4921 gattatctgc aaacgagcaa tccaaaaatt tttgcgtgtg gagatattgt ttcaaggaaa
     4981 accccaaaat tgacaccagt cgccactttt gaaggaaatt atgtagctaa gcggataacg
     5041 gatgcaacaa gcgaacctat caaatatccg attataccta cgatcgtcta cgccagtcct
     5101 aaacttgctg aagtaggagt tacaaaaagt cacgcctctt cgtcagatca agtggtagag
     5161 atggatctta ccagttggtt cacctatcat cgtgtcaatg aaccagttgc taaggctgaa
     5221 ttgacctttg accagcaaaa ttatttgatt ggtgctgcag tgattagcga gcaagcagac
     5281 gaattgatcg atgatttgac actcgtcatc aatcaaaaat tgactaaaaa agagttggat
     5341 agctatatca tggggtatcc gacattggca agtgatctct cctatttatt gaaataatca
     5401 gcatgataac taaagcgctt gttgcttttt ccttcttgtg ctacacttta aaaaatgaat
     5461 acctgtttct ggggatgtcg tagacgacat gagagtaagt tgtgaactta gacccattga
     5521 acctgtcagt taatactggt gaagggagaa acatagcaga cgcttgaagc gggttactat
     5581 gtcgtgccca tttcaagcgt tttttgttat tcatattaga agaaaggagg taacaaaatg
     5641 aagaaaaatc tttatccagc agtcagtatg gcattcattt taattatttg gcagattgtt
     5701 gtcatagttt ggaaaatacc tgcctttatt ttacccggac ctgctgctgt attttctgca
     5761 ttagcggcag atgcacaagt attactttcc cattctgtca cgacattaaa tgaagcagtg
     5821 cttggtttgg taatagctag cgttctttct tttattactg ccttggcaat ggattattgg
     5881 aagtttttag aaatgtcttt ttatccgtta ctagtgattt cacaaatgtt gccaatcatg
     5941 gtgttagggc cattacttac cctatggttt ggtttcggta tgcttccaaa agtgatcctt
     6001 gttgtcttga taagctattt tccgatcgta gtttcttttt cggatacatt gaggaaagta
     6061 tcaaaagagc aaatcatctt tttgaaaacg atgggggcag atacagggaa gatttatcga
     6121 atatataaaa taccagcggg tttatccggc ttcttttccg gtttaaaagt ggctgctact
     6181 tattgtgtca gcggagcagt cgttggagaa tggctaagcg ctcaagcagg cttagggtat
     6241 tacatgatac gcgtaaaaaa cagctaccag atcgataaag tttttgctgc gatcatttgt
     6301 gtcattctat tgagtttatt gctgaatggc gcttgttcat tattgaaaaa agggtattat
     6361 cgtatgcttt atatttttta agagaagggg atcaaatgaa aaaattatgg cttttattac
     6421 cgctactgtt gcttttatcc gcttgtggca ctgcaaaaga aacaagtagc aaacaagaaa
     6481 taaaggattt gaaaaaagtg acacttgttt tggattatgt accgaatact aatcatactg
     6541 gcatctacct agcaaaagaa aaaggctatt ataaagaggc tggtttaaat gtccaaatca
     6601 tcgaaccagg agataattcg acaagtattg gtctagtagg tgcggacaaa gcacaatttg
     6661 gtgtaagtta tcaagaagat gtcacttatg ctcacgcaga tggacagaat ataccagtca
     6721 aagcaattgc tacagtgatc aaacataata cttctggatt cgccacccta tcagacagta
     6781 acatccattc accaaaagat tttgaaggaa aaacatatgc aggttggcaa agtccaagtg
     6841 aagaagctgt cttaaaagct gtgatggaaa aagacggcgg ggacttttca aaactgacaa
     6901 tggtaggaag caatggagaa gggccagaaa gcttaggaaa atcctcagat atccaatggt
     6961 actttgaagg ttgggatatg atcaaagcaa aagaagcggg tattgaacta aactatatcc
     7021 cattgaaaga attagatgaa cggttagatt actatactcc ggtaattatt acgaatgatc
     7081 aactgatcaa aagtgatcca gaattggtac agtcttttat ggatgccacg aaaaaaggct
     7141 atcaggaagc tattaaggat ccaaatgaca gtgctaaact gttgcaaaaa tatgcaaaag
     7201 aaaatgatcg gacgttttta gaagaatcac aagcattctt gtcaaaaaat tatacggatg
     7261 atcctgagaa ttggggattg atggaagaaa aagtttggcg caattataca gcttttatgc
     7321 aggaaaacgg attgatcaag caagatgtac cttcgcaaga actattcact aatcagttta
     7381 taaaatagga gtgatagata tgtcaatcca aatcagccac ttgaatgtag tcattgaaaa
     7441 cctgaaaata ttagaagaca tccatttcac tatccctaaa gataccttta catcgattgt
     7501 cgcgccaagc ggagccggta agtcaacatt gctgaaaact ttaacaggag tccagcctat
     7561 gacttctgga acgatcaaag tagacgatca aaaagtaact ggtctccata cagcatttag
     7621 ttatatgccg caagaagata tgttacttcc ttggctgagc gtctatcaaa acgttacgtt
     7681 atatcaaaaa atcaatcatc taacaatcaa tgaagaacaa gtaagggatt atctggatat
     7741 ctttgggtta aacggctatg aacatttttt gccggaacaa ttatctggcg ggatgaagca
     7801 gcgaacggcc ttgttacgaa caatcatgaa tccttcttct tacttgcttc ttgatgaacc
     7861 atttggagcg ttagatgcta tgaccagagg gcagatgcaa gactggatgt tgaagctgcc
     7921 taaaaaagca aagcggacta ctttacttgt gacgcatgat atcgaagaag caatttattt
     7981 atcagacagg attcttgtgc tttccgcccg tccggcacat gtgattgctg agattcaagt
     8041 gcctgaaaaa cgacggtcac gtgaatggct actgcagcaa tcagagttga agcaaacgat
     8101 ttatcagtta ttggcaggtg aattagatgt taagtgatgc tcattgtcat ttagatggga
     8161 gtcagagttt agcactcctt cagcaggaac acacgatttt gacgatcatc aattgtgatt
     8221 cgccagaaga atggaaggaa aacagacagc ttgcagcaag taagactcaa gccttaagtt
     8281 acggtatcca tccttggaaa gcagatagct atacgtttga acaagttgag ccttttttga
     8341 aaaaagcaag gatcatcggt gaaatagggt tagataatat ttggacgaat gtaccgatga
     8401 ctacacaaaa aaaagtattt gagcgacagc ttgcttttgc agccataaat gagaaacctg
     8461 ttgtactgca cacaaaagga tgcgaaaaag agatcttaaa ttatatccaa gcttacccta
     8521 atcactattt gatccattgg tattcttctt tggactatca acaagactat attgatttag
     8581 gctgttattt cagcattggt cttgatttga aaaagaatcc agcagtctgg caactagcta
     8641 aagcagtccc aatcgaccac ttacttattg aaacggatgg actacatgca gcagagtggg
     8701 ctttaggggg gattttttca gaaaaagatt atgtctcact gcttgaagaa cagttaaaga
     8761 caatcgctga gatcaaagag ctatcagttg aagaagttaa aaaaaatact tatcaaaact
     8821 tggaacgttt cattagatta ggtaaataaa aaagagatgt gacacaacta gtgtcgcacc
     8881 tctttttggc tttctttgta taaaagtaaa ctgtttgatt aataaatgaa tcttagtacc
     8941 aaccgtttgc ttcccagaat gctttcgccg cttcccaaga accataacgt gaagtaacat
     9001 attgttctgc tactctttct tggtttgcag cagagtagtc accatttaag taagaagaat
     9061 ctaattggta acgtccgatg taacgtccgt tcgttgctgt gtaagaaccg cttgattctt
     9121 tttgtgcgat ccattctttt gcagagcttg tgcttgctgg agtagcagca gtttctgtta
     9181 cagcaggagc aggttgtgtt tcagcaacag gcgctgcttg tttttccgtt tgaaccgttt
     9241 cttgtacaac aggttgttca gcttggactg gtgcttcttg aacggctgct tgaggttcag
     9301 cagctgcttg tgcagagcct tctgttggga ttgttaattg ttgtcctgag taaatcaggt
     9361 taatatcaga aattgaattt gattctgcaa tggcattgat caaggagtta tcaccaacgt
     9421 atttttgaga aatcgttgat agagtatcac ctgattgaac ggtataagct tcgtccgcat
     9481 gagcagtcgt tcccatgaag aatgcagcgc cggcagccaa agttgttcca aaaagtaaag
     9541 ttttaagtga tgtcatgaaa aatctccttt attcgttaaa aagttttatt tgttgtgttc
     9601 aacgaggaat actttaacac gtgaaaatat ttcataggta aaagaagcat gacaatttat
     9661 gacaaatcca cgttttttgt aacagaatga tgttcgctcg atgtgaaaaa tgatgaggaa
     9721 tttcacataa tgcagaaata atgggattag aacaggtgtt ctgatgagaa aagaaatgta
     9781 taaataactt gaaagaaatt gcttttgttc atcattttat ttacatattg cgtatatttt
     9841 ttgtattttt gcttgagatg ttacaataaa agaaaaagga ggcggttcca atgagtaaaa
     9901 gataccagaa catcatggta gcagttgacg gatcaagaca atcgatcaaa gcgttcgctg
     9961 aagcccttga cttagcaaag gacaatgaag cccacttatt tatcgtgtca atcatcaaca
    10021 aagttgagct gacccatagt gcctatgcct tttcaaaaat ctatgcagat gaaaaacaaa
    10081 gaacagaagt agccatgctg aaaaaaatca atgatgccaa agagtacggc attgctgaga
    10141 tccacgcaat agttgaaact ggagatccaa gaaatttgat tgccaatgtc atccctggac
    10201 aagaaaatat tgatttgatc gttatgggag cgacaggaaa aggtgctata caacaagcat
    10261 tagttggatc gaccgcttct tatgtcgtat cacatgcccc ttgcagtgtg ttggtcgtga
    10321 agtaatgaga taagggagga atagaaaatg gttcgtcctc aagaagtccg tgcgccaaaa
    10381 gaaaaaatag aaattttagc aattattgaa gacggcacac aaacaaagaa gggatactcg
    10441 atcgctttag taaagtggaa aggaaaaaaa ggtgttgctc tgagatggga tggagataac
    10501 caacaagata aaggattccc tgtcacagct aacggttatc atccagcgtg gtttgttctg
    10561 cctgataaat ttatggaatt atacagctat gattatgcaa gagccgcagc atcaatcaaa
    10621 gcattagata aattaacaga agagcagaat gaaatggagg aaaaataaaa aagacgaacc
    10681 aatcagcctt tagaaaactg attagttcga ggagtaaaaa tgaaaaaagg ttatttggga
    10741 tgaaactaga ataatgcgta taacttaaat aaaacttaaa gcaataaaat ggcaagaggg
    10801 tgaggcaaat ggaagcagga aaagtattac aggaagtaaa agcactatgt gcagataaaa
    10861 aatatgaaga agcgaaaagc ctgatcgaaa agaacaaaga aaatttggaa gaaaagtacc
    10921 cgatcgcaca agaatttata gaattaaaac aaggaacgat ccttcaccga ttcaaacatt
    10981 tttttggtat aacagaatag aaaaaacgat taaagatcgg cagaaatttg ctgcatcttt
    11041 aatcgttttc tttttaaaca tgcgagggat tcaattctga ttcttatata aaaaatagat
    11101 ccaaataata agaaaaatta ctgaaagcgt caattcgatg atgtgtgtta ttaatacaga
    11161 cgttgtttcc caatgcctaa aagaaaaaat ttgataagta aaatatacag caggtaaaga
    11221 ggccagtaaa actgaaaact tcttttttgt ccattttatg taatagaaaa gaatgcttat
    11281 cataataaaa aaaggaatat aaaaaacaaa ttgaccgatc gcgatcatac tattgcctcc
    11341 gataaataaa gtccatgata caaaataaca ctttgtgcga aatggcgcaa gcagttttgt
    11401 ggatttatcc ggtaaaatct gtatcgattt tgaaattaga ggagaaactt gctataatca
    11461 aagtgaaaga aggagtggga ttttcatgga tcatgtagtg ttagaaatcg atttgcattt
    11521 gatcaataat attcgggtga tttactatgt tgtaagtaac agtgtcgaac aacgtgtact
    11581 gacgaataaa ataaatggta ttttagcaaa gaaagacgtt caccgattca ataatggtga
    11641 aggaagttat tatagtatcc cagtagaaaa aattatttat acgacagtca aagtcagaga
    11701 agatctagat accaaaaaag catatgaacc tatttttaca acttattgat tgacagcaga
    11761 tggaggaaaa aaatgaatcc atatgaacaa cttattcaag atttgatcga tggaaaaatc
    11821 gaaaagattg atgtaaaaag agaagaatta atggcttttc gagaagcgtg gctgaagttg
    11881 gagaatcgta aatatgtggt gggaaaagcc tcactgaacg gcaatgtgac ttatcattac
    11941 gatccgacac gtttatttta gtatgaagta ttcagaaaag aaccaaagga ggacgatgca
    12001 gcagcattgt cctcctttga ttttacaaaa gtgaaagttt tacttatttg aatagatttt
    12061 taaatttgtt tttgacactg tcgatattta catcttttaa aagattttgg gctttctctt
    12121 tataaggtcc taattgttct ttatgttctt cgataaatcg ttttgcgcca tcgaaattgc
    12181 cgttcttaat catttccttg actttttgaa ataattcttc ttgattcatt ttgaacacat
    12241 cctttgctat caacatgatt ttttacatgg acaaaacgag aaaaactgat ttatccacac
    12301 gtcaataata tcaaataacg atcattttta ctaaaaaaac actcgattct cgcttctaaa
    12361 gtaggagaaa aatctccaac ttgggacgga tggaatttct ttaactgacc catatactga
    12421 atatataaca aggaggtaga aagatgaaag tgattaaata tttgattgga ctggcggcga
    12481 ttagtggtat tttgctattt ggactgaaat attatactga aggaagttat ggagaaatcc
    12541 ctggtatact tgatcagcta aatccattag ttgaaaaagg agaggtctat gtaaagacac
    12601 aaaaaccaga ggaagtaaat gaatatggta cagcaaggta tgtgcagaaa gctgctgatg
    12661 ccaatgggaa agaacgaacg gttgaattta atggtctttc tgtgttaaaa gaaaatcatt
    12721 atttgaaaat tagcaataaa ggagcacaca tagaaacgta tgaggaagtt cctagagaaa
    12781 aagttccgga gaaagcatta gcaattattg ggtaaatcag tttttgtatt aagaatacgc
    12841 ccgattttat tattcataat ttaattatgt aaactaaata caagtagtaa aaataataca
    12901 ataatccccc tgcaaaaaga ttgttctttt acagggggtt cattttttca gcaagcacat
    12961 caaattggga tggcagggtt cgaacctaca attgcttgat tcagagtcaa gtgccttacc
    13021 agtttggcta catcccattt attcaacaaa ataatcatag cactttccta aaaaaacgca
    13081 agttattttt cttgatcgga tagttgttgc tttactcgta aaaggcggcg tactagtttc
    13141 ttttctaatc cagcaggaag ggcaatcagt tctgctactg acaagacggt gtccaataac
    13201 tgatattctt tgacaggttc attcataata tgatagatgt ttgctaaacg ttcaatatat
    13261 tcaaagcgag tatactctgt tcgagcaatt agaaatttca ataaatcggt ggctttctcg
    13321 tactcttgat ctgcgtaata aacaagagat tgacgataca atttgatttc cagttcattg
    13381 agggtagcag gtctgcgacg ctcgttccac tcattaatag agactttacc ttgaacattt
    13441 cccttgcagt cacggacaaa aataatcgga atttcatttg gatccatttt tttatgtgtg
    13501 aatttttcca ttatctacgc tcccttcaaa ggtttggtat cttgagtata agggattttt
    13561 gtttgaatta ctagcttttc tccaaagtta gcatctgtcc acccattgca gtgaaaatcg
    13621 ccgaaaaatc gttgatcggt actgctcgat tttttacacc atagggatcg ttgacataaa
    13681 tattttcttt gtcatatcca gtgactacac aagcatgaac aagaggagtg actcttactt
    13741 ctccattatt tgtggtccaa gtcataaaat cagtttcatc tgggacttca aaattgatag
    13801 tcacagaagt ccagacagga tttcctttag atagtgcttt tttgatatca tcgagttgag
    13861 catcggtttt tggaaaagtg tgataatccg tttggacgat ttgctgagca agggaggcaa
    13921 ctgggggaac atagacgacc atagcccagt ctccgccact gatatcacca acaaatcctt
    13981 cattaggatc gccatgagtc ccatctgcat tgaaaacagg gacgtaatcc aattgctcag
    14041 ctaactggtt tttagttgtt tcataaccat aaaaagataa aagcatagaa agcgaagtga
    14101 cttcacagcc attttctaaa gaaggagaat caaactggct ctctaatgga acatctagta
    14161 aaacttctgc tttctctttt gtctttttcg ttttttgtgt tgaacttttt tgtgcagtgt
    14221 tcattgtatg ttctggagaa tggatatgta ggtaataact tgtaccgctt aataaaagag
    14281 cagctgtgcc ccctagaaac aatagttttt tgaacgtttt tttcttcatt tgaaatctcc
    14341 cttttcagct gtttatcttt cctattataa atgtattttt attggaaatg gaagtattat
    14401 attttaatat gtaggaaata attagttttt aaggaatatg ttttaatttt ttgaaaaaaa
    14461 ttcttctaaa tacaacagct gttttaattg tgaaaacgaa aacagtacgc tataatgcct
    14521 gtaaggttga tttttaactg taaataaaaa tcaatgagtt aaatctccaa agaaatagca
    14581 gtggtaatgt ggagaaaagt tccagcagtt taggaggatt ttgatgacgg aagaaatgat
    14641 taatttagga gaacaatatg catgcaaacc aattggtttt acaaaaacgg tcattggtga
    14701 agttgtatca aaaatgacaa actgcgccgt ggtcaaagtt gcccaatgtg ctgcagagga
    14761 ccaagaatta ttagatgaaa aagcaagcat ggttgttgca aaatatgata catttgaata
    14821 aagaaaacaa ttggacgtgc ggcgtgctgc aagtccaatt tctttgagaa ggtaaatcag
    14881 tattcttttc ctattttctg gagaagaaaa agcgaggcga gttgtcatgg taacattctt
    14941 tacggttcta ttgtttttat tcgttgttgt gatgttttta tttctttggc tggttcgaaa
    15001 agaaattatt tatcggactg tgcgaaatcg atgggtctac ttggtgatac ccttcttagt
    15061 gtcactagtc atttggtata cactcatcag tcatcctaca gcagatgaat tggcaaaagg
    15121 gatcttgtca gcaatgattt ttattagttt tttgttggac agtagaggaa tcacagaaga
    15181 aggacttgtt ttgaatagct ttgacaaaaa aggtgttcct ttttctgaga tcaataaaat
    15241 tgtattatat cagccaaaag gttctaaaat cgtaaaaatg aattttttcc gaaacggatg
    15301 gcgaggaccg atgctgaaat tttctgcatc attagaagaa ttagttccat ttttgtccca
    15361 acgcttaaac gatgaggcag agattgatat tatgattgat ccagaataaa aacagaggtt
    15421 gcgacaagat gtttgtcaca acctctgttt tttatttcaa ataaaattat ccaaatacgc
    15481 ggaagcgatc ggcatcgttc atgtattctt tttccccagc cggtgtttct ggtgaaccga
    15541 atactaattg cgaacgtagt ttccagtttg aagggatatt ccattctttt gcaactgctt
    15601 cgtcaataac tgggttgtaa tgttgcaagt ttgcgcccaa tcctttatca actaatgcta
    15661 cccaagtatt agctgttgcg attccgtttg attgttctga ccaatctggg aagttgtctg
    15721 catataattc aaattgttct tgtagacctt ttaccacgtc agtatctttg aagaataata
    15781 ctgtaccata accatttttg aaaccagcta atttgttttg tgtatttggg aaagcttctg
    15841 ctggagtcaa tgggcgaagt gcttcttcag tgatttccca aagtttttca tgtgcatctc
    15901 cgaaaaggat aactgcgcgt gttgattgag cattgaaagc agttgggctt tctttaattg
    15961 cttctttgat caaagttgtt aattcttcat tagaaagtga aacattacgt cctagatgat
    16021 agattgaacg gcggtttttc aaagtgtcag taaaagttgt cataataaaa tttctccttt
    16081 gatttgcgtt ttgcattttt ttaattgatt acgaaatgaa gtatagcacc ttacaaaaag
    16141 taagtaaaca gatatgctca caaaaaataa gcgaagttca aaaaacaatt ttaataagaa
    16201 aaatcgttat gtaaggattt tcttagttat cgctttcatt gttgtgtttt aggaattttt
    16261 gcttatttga aaaaaacagc tgatatataa ttattaaatc tctatcttct tttttttatg
    16321 atattgccat taaaaattac ttgcttttat agaaaattag tttatatttc ttaaataagt
    16381 tttttagtta aatttttcgg taaagatgta taatattttc tgaaaattta aagaattggg
    16441 gtatagatta tggaaaaaca gcaagacaag ggcttcttcg ggcagccgaa gggattgtca
    16501 actttgttct tcactgagat gtgggaacga tttagttatt acggtatgcg tgcattactt
    16561 atctattata tgtacgatac agttgccaac gggggacttg gattaccacg agcaacagca
    16621 ttagctatca tgtcgatcta cggatctttg atctacatgt ctagtatcgt cggcggatgg
    16681 gtatctgacc gagtgttagg agcaaaacgc actgtattct tcggtggagt tttcattatg
    16741 cttggccaca tcgtcttggc tttaccattt ggtgtaagta cattgtttgt ttcgatggca
    16801 ttgattatta tcggaaccgc ctttttgaaa ccaaatgttt ctggcatggt cggacatctt
    16861 tattcaaaga ctgacttgcg aagagatgca ggattttcga ttttttacat gggtgtcaac
    16921 ttaggggcat tattagcacc actaatcgtt ggtacaatcg gtcaagaata taactatcat
    16981 ttagggttct ctttagctgc tattgggatg ttctttggat tattacaata catcatccaa
    17041 ggacgcaaga cattagacgg agtgggtaca acaccgccga atcctttgac aactgaagaa
    17101 cgtaaaggat tcatccgtaa cttgatcatc gcacttgttg ttatcgctgc agtattcggc
    17161 ggggcacaac taactggtca tctgacaatt gacttttttg tcaatacaat cagtgtattg
    17221 gggattatct taccgttgta ttactttatc aaaatgctga cttcaaaaga tgtaactgct
    17281 gaagaaaaac caagagtatg ggcttatatc ccattattcc ttgcagcaat cattttctgg
    17341 tcattacaag aacaaggttc atctatctta gctttatttg ctagtgaacg tacacaatct
    17401 acgctatttg gtgtaacgat ccaagcaagc tggtatcaat cattgaatcc aattttcatc
    17461 tttattctga caccagtatt cgtaacgatg tggacgaaac ttggtgaacg tcaaccatct
    17521 acagtagtga aattctctct tggactttta tttgcaggat tatcgtactt gctattgatg
    17581 ttgccgggta tgctttatgg tacaagtgga aaagtaagtc cattgtggtt gttgggaagt
    17641 ttcttcatcg tggttattgc tgaattgtgt ctttcaccag ttggtttgtc tgtgacaaca
    17701 aaattagcac cacgagcatt tgaatcacaa acaatcgcta tttggttctt agctgatgct
    17761 tcttcacaag caatcaatgc acaaattgca cgcttctaca caccgggaac agaatctgct
    17821 tacttcggta ttatcggtgc agtagcaatc gtcggtggta tcttgctatt tactgtgaaa
    17881 gaaccaatca aaaaactaat gggctctatt cattaatcaa tgaacataaa aacaggaatt
    17941 gcttatttgc agcagttcct gtt
//
LOCUS       DACDXE010000064        17231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.064, whole genome shotgun sequence.
ACCESSION   DACDXE010000064 DACDXE010000000
VERSION     DACDXE010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.064"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..370
                     /locus_tag="IWM23_002147"
     CDS             2..370
                     /locus_tag="IWM23_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9884758.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            550..1491
                     /locus_tag="IWM23_002148"
     CDS             550..1491
                     /locus_tag="IWM23_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAP9884759.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1560..2291
                     /locus_tag="IWM23_002149"
     CDS             1560..2291
                     /locus_tag="IWM23_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAP9884760.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2435..4615
                     /locus_tag="IWM23_002150"
     CDS             2435..4615
                     /locus_tag="IWM23_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAP9884761.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4635..5117
                     /locus_tag="IWM23_002151"
     CDS             4635..5117
                     /locus_tag="IWM23_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAP9884762.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5133..5894
                     /locus_tag="IWM23_002152"
     CDS             5133..5894
                     /locus_tag="IWM23_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAP9884763.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6025..7359
                     /locus_tag="IWM23_002153"
     CDS             6025..7359
                     /locus_tag="IWM23_002153"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAP9884764.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            7666..8151
                     /locus_tag="IWM23_002154"
     CDS             7666..8151
                     /locus_tag="IWM23_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAP9884765.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8144..8827
                     /locus_tag="IWM23_002155"
     CDS             8144..8827
                     /locus_tag="IWM23_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAP9884766.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            8953..11598
                     /gene="polA"
                     /locus_tag="IWM23_002156"
     CDS             8953..11598
                     /gene="polA"
                     /locus_tag="IWM23_002156"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAP9884767.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            11643..12479
                     /gene="mutM"
                     /locus_tag="IWM23_002157"
     CDS             11643..12479
                     /gene="mutM"
                     /locus_tag="IWM23_002157"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAP9884768.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12443..13072
                     /locus_tag="IWM23_002158"
     CDS             12443..13072
                     /locus_tag="IWM23_002158"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAP9884769.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13124..13456
                     /locus_tag="IWM23_002159"
     CDS             13124..13456
                     /locus_tag="IWM23_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9884770.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            13768..14259
                     /gene="nrdR"
                     /locus_tag="IWM23_002160"
     CDS             13768..14259
                     /gene="nrdR"
                     /locus_tag="IWM23_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAP9884771.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14282..15655
                     /locus_tag="IWM23_002161"
     CDS             14282..15655
                     /locus_tag="IWM23_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAP9884772.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            15655..16581
                     /gene="dnaI"
                     /locus_tag="IWM23_002162"
     CDS             15655..16581
                     /gene="dnaI"
                     /locus_tag="IWM23_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAP9884773.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            16710..17150
                     /locus_tag="IWM23_002163"
     CDS             16710..17150
                     /locus_tag="IWM23_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9884774.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
BASE COUNT         5877 a         3008 c         3796 g         4550 t
ORIGIN      
        1 gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa aaaatgcaac
       61 gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg ggacgcttat
      121 aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc gagcatttgt
      181 tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt acagtaaaga
      241 tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg atatggtaca
      301 gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact ttgcggttac
      361 aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag cgaagtcttt
      421 ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt atacacaagc
      481 aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa agaaggaagg
      541 aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat cgaagaacca
      601 gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa aggtcgtatt
      661 ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa cacgactgcc
      721 tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa gatcgatcca
      781 agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct tgtaagttta
      841 aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc accgcaagaa
      901 tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa tgattatgaa
      961 tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt tcgagagctt
     1021 ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt gacctactgt
     1081 actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg attcaaagat
     1141 gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt ccaaggagaa
     1201 ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat caaccaagta
     1261 gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg atatatcaat
     1321 tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga agaaaaacat
     1381 ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt tcaagaccat
     1441 ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata gaaaacaata
     1501 gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg gtgatccaca
     1561 tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt tttgaaaaac
     1621 ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat ctcaatgatc
     1681 gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa gaaacaggag
     1741 ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa tcaccggagg
     1801 actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc ctgattcacc
     1861 caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa tcgagatatt
     1921 cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa aattatcttt
     1981 ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt ctaagagact
     2041 ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag acaattgtat
     2101 ttggacatac gattacacca atgctccatg gagatatgca gacaacagac ctctggattc
     2161 aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg catggggttg
     2221 tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa ggaccttggc
     2281 atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc tctttttatt
     2341 tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta tgatagaata
     2401 aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga accagacgat
     2461 cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga ctactgtact
     2521 taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa aagaaatgac
     2581 tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct atcttgaaaa
     2641 cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga aactgactcc
     2701 cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag atttgtatcg
     2761 tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag gacttgaacc
     2821 actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag cagaaaaata
     2881 tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg ctcatgagat
     2941 tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt cttatacgta
     3001 taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa aaggcgtata
     3061 tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc gaatattagc
     3121 tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag atgaagcagc
     3181 aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt cttcttcatt
     3241 cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa tcgagcgaga
     3301 actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt tcggcgaaaa
     3361 cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg gatttgatcc
     3421 ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag ttttagcaat
     3481 cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg ctggaccagc
     3541 ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa atggtacagc
     3601 cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc ataaaaccta
     3661 ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa ttgcacgaaa
     3721 agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc gtcgcctgca
     3781 agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag gccagtacca
     3841 acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg aaacagcggt
     3901 taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc atatatcagg
     3961 attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg gagaattcac
     4021 cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg aacaagcaat
     4081 cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta tccatcccga
     4141 aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa aagagcttgg
     4201 tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg ctgaaagcct
     4261 atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac ctggacgaga
     4321 tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta tggaagactt
     4381 gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg gcgcatttgt
     4441 tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca aaaaattcgt
     4501 caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga tagaacaagt
     4561 cgatacgaaa aaaggtagaa tcagtttgac gatgctttcc ccttatgaag agtaacaatg
     4621 aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag atatcattaa
     4681 aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta cgtacaactg
     4741 gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta tttgaagagt
     4801 cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat catcttcatc
     4861 tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg aaaaaaacag
     4921 gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag tattatcgat
     4981 gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc aaatatcgtt
     5041 gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac tttaatgaag
     5101 cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag aggaatctaa
     5161 aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat tttgaggaac
     5221 tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct gataaagcag
     5281 cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg gtggggcatg
     5341 cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa gatggcatga
     5401 gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc gataaggctt
     5461 ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca ctggagattc
     5521 ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg acactgcaac
     5581 gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt ttcgcgaatg
     5641 ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt acagcaaaag
     5701 aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga gaaatggaac
     5761 gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt cctcacattg
     5821 atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata aaaacggatt
     5881 tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat ttggttgaaa
     5941 ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa aagaatggac
     6001 gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca ttttgttggt
     6061 atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg tctaaatgtc
     6121 caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa agcaaatatc
     6181 acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat tgcaggaaat
     6241 gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt ggaagtgatt
     6301 cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc tgtaacaggc
     6361 tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg tgtccgtcca
     6421 acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga attcttttca
     6481 tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta tgcaatcatg
     6541 accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt atttacagct
     6601 ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga cgatgcttat
     6661 ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga aaacgatgat
     6721 atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt ctatcatggg
     6781 gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat cttaaatgct
     6841 ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt tgcagaagaa
     6901 atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc agacatgacc
     6961 gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga tggcgcacgc
     7021 caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt tacgcgtacg
     7081 attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt ttacttatgc
     7141 gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga ccttggagcg
     7201 aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt gttagattat
     7261 catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga acaagcgtat
     7321 gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg cagtgtcctt
     7381 ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt ttgcaactta
     7441 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa tcaaaaacat
     7501 gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt tttcacgacc
     7561 tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt acgctacttt
     7621 tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa cataggtaag
     7681 ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt atccttaaca
     7741 gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga tgcaaatcct
     7801 ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat cgttccatca
     7861 gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg accaggatca
     7921 catgttgtca atttttctgt caattttatt gaaccggttt tccattatga aacactgact
     7981 tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat ttctgtagaa
     8041 gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca accgccacaa
     8101 gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata atccaagtac
     8161 atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag gtatgggact
     8221 tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc aattagcaac
     8281 ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac tgattttagt
     8341 aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg gatttattgc
     8401 ctactctctt ttgaatggca taacattagc ggttactctt gcatactacg atattggaac
     8461 agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg ttgtcggagt
     8521 aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt taatcggagt
     8581 gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg agctgttcat
     8641 ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc aaagcatccg
     8701 taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat tcatggcatt
     8761 gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct ttggacggaa
     8821 caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg gtatggtccc
     8881 gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac tttacagtaa
     8941 ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag tgtggctttt
     9001 cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa cggtcttcat
     9061 acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa agagcaaccg
     9121 actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga aatgtatgcg
     9181 gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat gccatatata
     9241 cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta tgaagcagat
     9301 gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt cgtcgtgctt
     9361 tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga tatcactgta
     9421 aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa atatgatggg
     9481 ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc tgacaatatc
     9541 cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca atatggctca
     9601 gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa agaaaatctg
     9661 ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa tacttcagcg
     9721 cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga taaactcgtt
     9781 ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat cgtagaagaa
     9841 gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga ggaaatgttt
     9901 actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac agaggaaatc
     9961 gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct atctgtattc
    10021 gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa agtatatgat
    10081 gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg aatcgatttt
    10141 gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga tatcgaagga
    10201 atagctgctc attacggata caacgatatc caatcagatg aagctgtata cggcaaaggg
    10261 gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc gcgaaaagta
    10321 gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa aaaccaagaa
    10381 gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat ggaaataagc
    10441 gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc tgctcgctta
    10501 agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt gaattcacca
    10561 aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa aaaaacaaaa
    10621 actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc accaatcgta
    10681 gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt cgagggactg
    10741 ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac attgacccaa
    10801 acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg attagatgaa
    10861 gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat ttattcttct
    10921 gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga acatttgaaa
    10981 gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt atttggaatc
    11041 gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt gaattttggt
    11101 atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag ccgaaaagca
    11161 gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga atatatggaa
    11221 agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca ccgccgtcga
    11281 tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga acgaacagct
    11341 atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat gatcgaattg
    11401 gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt tcatgatgaa
    11461 cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt gaaggaagtt
    11521 atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg gggaaaaacg
    11581 tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg tgaaaagaaa
    11641 aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg ttagtagtag
    11701 gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa ccagagacac
    11761 ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt agagggaagt
    11821 ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg gaaggaaaat
    11881 atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt ttcttttttg
    11941 aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg acaatcgtcg
    12001 aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag cctttgccag
    12061 atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa gcaatcaagc
    12121 cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta gatgaagcat
    12181 tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc aaagaggtag
    12241 aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc ggaggtacca
    12301 cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta gcactgcatg
    12361 tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg aaaacaaaag
    12421 tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc gcaaagtagg
    12481 atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag ccaaagtctt
    12541 tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa ctgttgagcc
    12601 tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat tgcagcctga
    12661 tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg aaaaacggca
    12721 aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc aaatcgcctc
    12781 tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg aaggtcatta
    12841 tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc agcttcagcg
    12901 tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta gtagtcaact
    12961 tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg gaacaaaaga
    13021 aaaacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt gagtatctgt
    13081 gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat cattaaaaaa
    13141 tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc tcaataaaga
    13201 aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag aaaagcagta
    13261 ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga agcggcaaga
    13321 tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc tcccagaaag
    13381 tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact atacaggaat
    13441 cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga tgagaaggtg
    13501 ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt aagttatttc
    13561 tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag gggacacaat
    13621 tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag gacagtcgcg
    13681 tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca tcctttatat
    13741 aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca taataattca
    13801 cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg tcgcgaatgt
    13861 gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc gttattagta
    13921 atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag aggactcatc
    13981 cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt caaccatgtc
    14041 gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat tggtgaatat
    14101 gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc aagtgtttat
    14161 cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt ggataaagca
    14221 aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag gaggcatttc
    14281 cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa gaaaagcggc
    14341 tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac taatcggcgg
    14401 agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat ctggcaaagg
    14461 tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc cgcaatttta
    14521 tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta aagaagatag
    14581 tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta aagcattttt
    14641 caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac gattccagaa
    14701 gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa aaatcacaaa
    14761 aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc aagaaaacat
    14821 cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg aagcagattc
    14881 gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg caagtcttga
    14941 tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg atgaattgac
    15001 acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat ctgaaaaaga
    15061 actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa aagaaacagt
    15121 tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa acgcagcgct
    15181 tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag caatcaagtc
    15241 agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc ttgtttctca
    15301 atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag tgatcaagaa
    15361 taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg cacaaaagaa
    15421 aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc aagcaaaaca
    15481 atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc gacgggaaaa
    15541 gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg agaaaaaagc
    15601 agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata tctagtggaa
    15661 gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga aaagtatgct
    15721 gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag cgcacaccaa
    15781 gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga attcgtgcaa
    15841 gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta tgagccgaag
    15901 ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc attgttagca
    15961 cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc aaaagatatt
    16021 caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc gagcttagaa
    16081 gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa aggactgtat
    16141 ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc gagagaatta
    16201 gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt tgagatgaaa
    16261 caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg ttcacctatt
    16321 ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga cgaagtattc
    16381 ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc atcgaatttt
    16441 acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga agagccgcta
    16501 aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga aatgactggt
    16561 cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac ctagtagtaa
    16621 aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac cgcaacaagt
    16681 aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt atatctgtag
    16741 ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca ggttctgttg
    16801 attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag agacaaagca
    16861 tatttgtcgg aacgattgaa agtattactc aagtagaagc agaagaagaa acgcttcagt
    16921 tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg tcattcagca
    16981 atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata aatgaattga
    17041 aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg atgtctatcc
    17101 caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa ccaataacat
    17161 tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt ttaaggagat
    17221 atgggaattt t
//
LOCUS       DACDXE010000065        17210 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.065, whole genome shotgun sequence.
ACCESSION   DACDXE010000065 DACDXE010000000
VERSION     DACDXE010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17210
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.065"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(97..1116)
                     /locus_tag="IWM23_002164"
     CDS             complement(97..1116)
                     /locus_tag="IWM23_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884775.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1138..2145)
                     /locus_tag="IWM23_002165"
     CDS             complement(1138..2145)
                     /locus_tag="IWM23_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAP9884776.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWM23_002166"
     CDS             complement(2138..2803)
                     /gene="pgmB"
                     /locus_tag="IWM23_002166"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAP9884777.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2796..5090)
                     /locus_tag="IWM23_002167"
     CDS             complement(2796..5090)
                     /locus_tag="IWM23_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAP9884778.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5350..7515
                     /locus_tag="IWM23_002168"
     CDS             5350..7515
                     /locus_tag="IWM23_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884779.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7819..8652
                     /locus_tag="IWM23_002169"
     CDS             7819..8652
                     /locus_tag="IWM23_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAP9884780.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8754..10454)
                     /locus_tag="IWM23_002170"
     CDS             complement(8754..10454)
                     /locus_tag="IWM23_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884781.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10961..12121
                     /locus_tag="IWM23_002171"
     CDS             10961..12121
                     /locus_tag="IWM23_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAP9884782.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12137..12580
                     /locus_tag="IWM23_002172"
     CDS             12137..12580
                     /locus_tag="IWM23_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9884783.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13063..13593)
                     /locus_tag="IWM23_002173"
     CDS             complement(13063..13593)
                     /locus_tag="IWM23_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAP9884784.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13698..14249
                     /locus_tag="IWM23_002174"
     CDS             13698..14249
                     /locus_tag="IWM23_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9884785.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14402..14875)
                     /locus_tag="IWM23_002175"
     CDS             complement(14402..14875)
                     /locus_tag="IWM23_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9884786.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15013..16485
                     /locus_tag="IWM23_002176"
     CDS             15013..16485
                     /locus_tag="IWM23_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9884787.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16482..17192
                     /locus_tag="IWM23_002177"
     CDS             16482..17192
                     /locus_tag="IWM23_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAP9884788.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5352 a         3094 c         3533 g         5231 t
ORIGIN      
        1 tttttacaga taaacaatgt gttagaagta gtatcttctt ttacataaaa agctgaccat
       61 aggataaatg gtcagcgtat ttagtgagtc agctatttag tatgggtttt ctaaaggaac
      121 gactgtctct cttttgatca agcgatgagg gatgaccaaa cgtacgccac tgctcaattc
      181 ttcatggatc aattcgcgca gcttttgcat agcgacacgt cctaattcgg agatatcgat
      241 atcgatactt gtaaggtaag ggtgtgttaa tgttgagaag atcgagttgt tgaagctaat
      301 gacggagagt gtctctggaa tgacatagcc atacatttgc gccaactgca tcgtgcgaag
      361 ggcgaagata tcgtcaatga ccaccaatgc tgtggcttcg ttttctttca acgttttctc
      421 aaaggacaga taatcctccg gatgctctag tactacactg gataacgcag gaagaccagc
      481 catcatcatt gctttttgat aaccgaaata tcgttcgaaa tacaaacttt catgagtcgt
      541 gtttgtcaca aacaggatat tcttatgtcc attctggatc aaaaattctg ttgcttgttt
      601 acctagaagc tggttatcat tatctacata aacgatttcg ttctcatgat gataaggctg
      661 tccgatcaaa gtgaaaggga tgtggttttg gtataaataa tcaatgacag tatcttgact
      721 atctgaatac actagaataa aaccatctac ttgtttttgt aaatgcatac gctggacgtt
      781 ctctaacagc aattcaaaat ttttagcagt agcgattgct gtagtcatat cataattccg
      841 cgcttcttcg ttgattgctt ccatgatctc aagataaaat gggttaccga tccgctcttt
      901 tgaatcaaga gggggtaaga ttacgccgat ggcactggaa atgcgttttc ctagattgcg
      961 ggcagtcatg ttaggaacat aacctagttc atccataatt tttcggactt tcttctttgt
     1021 tgtctcggaa atactaggat gatcgttgat gacacgagag acggtcgagg tggcaacacc
     1081 ggcttttttc gccacatctt ttacggtaat agccatgata aaaacacctt tctcttgtta
     1141 ttgtgcagag aaccgataaa tcgtctgact gatccgttct tgaccaggat ggaggttgat
     1201 cgatccaaat ttggggaaat gaacgatatc agggatttct tgcggttcga ttgctaatcc
     1261 gtagtttgaa tgcatctgtt ttccattgac agagaaatca gcttcgaatc cagtcgtaga
     1321 aaataatacc atacttttat tagaagttgc tatagtcatt tggcgaccag aatgcttctc
     1381 gaataattgt aaggaagttt tggaaagcct aggatgatgc aaaagaaata catcatctag
     1441 tccatctgga taacatgcaa ggatctcttg cagtgttggg gattttctaa agtcgattgg
     1501 cagtttttct ttttttgtcc aatttccagt aggcagtttt tcttgatcta attcaagcac
     1561 accatctaaa aagaccgtta actgatgttc atagatgtct cttgttccat ttcctgaaag
     1621 gttgaagtag gcatggttgg tcggattgca gatggtttca acaaggctac tagcagaggt
     1681 tgtcatctct aattcgtttt ttgttaaacg gtaagtgatc gtagcagtga ttggtcctgg
     1741 ataggtagaa aattcatctt ttaagtaaaa gaccacacct attgatgatg gagttttgaa
     1801 aacttctact tcccaaaatt ggaaagacca gcctttcgtg ccaccgtgga tatggtgtga
     1861 tccgcagttt ttctccaatc tatgctcacc ccaagaagca tcccggatcc ttccggctac
     1921 aggtccgaca gttgctccaa aaaagctttt gtctgttaga acatcttcga aacgatcgta
     1981 agacaataaa acattttctg attttccttc tttgtctggt gcgaagattt ggtgcaggcg
     2041 ggcaccgtaa ttgagaagaa cgacttctaa gtagtcgttc ttcaatgtga ttttctttat
     2101 ttcagtctct ggaactggta cgacggagat ctctggatta tccatggtca tgccactttt
     2161 ctttcaagta atcgatcgta agaaaagtcg tatcagggac aagcgggatc tcctctccaa
     2221 ggtcttcagc acgtcccacg ccaattggtt gagcgccact tgagaggatg gcttggatac
     2281 cggcttgcgc gtcttcgatc ccgatacatt cttctgcttt tagtccgaca gcttttgctg
     2341 caaggaggaa gatatctggc gcaggttttc cgtttgttgc ttctgccggg tcggcaatag
     2401 catcaaagta aggtgtaaga cccatttttt caagtaggaa tggcccgttt ttacttgcag
     2461 aagcaagagc aatcttgatt ttttcttctt ttaaagaatc cagtaaaggc acgaccccgg
     2521 ggaagacatc ttttggttcg atcgtctgga tcatctctaa ataataatcg ttttttcttt
     2581 tcgctaattc tgcaaattct tcctttgaaa aagagtgttc ttttccgcca taagctaaaa
     2641 gcaaagagag tgagtcttct cggctgacgc cttttagctg ttcgttgaat gtgtggtcga
     2701 tcgtaatgcc gatttcctga ccaagctttc gccaagcacg ataatggaat tcagcagtat
     2761 cggtaatgac accgtctaaa tcaaacaata cccctttaaa catttgcttc ctcctttctt
     2821 agcgttattt ctagacgatc cgctaaagaa taagactgtt catagacgta aatttctaga
     2881 ggagaacctt ctaataactc gaaagtaact tgttcatctg tgactgagat gaacaataaa
     2941 cgtccgcgat aattgatatg gaaagaataa gctttccaag tttttggcag aaatggtgcg
     3001 aatctcaact gatcatgggc agttttcatt tgagcaaatc cttggacaat cgacaaccag
     3061 ctaccagtca tggaagtgat gtgcaagccg tcttctgtgt cgttattgta attatccaaa
     3121 tccaagcgag ctgttcgctg gtacatttct acagctttgc tttccattcc taattctgct
     3181 gctaaaatcg aatggatact tggcgaaagg gaagactcat ggacagtcat tggttcatag
     3241 aaatcaaagt ttttttgctt ttcttctttt gtaaaagtat cattgaagaa ataaatcccc
     3301 tgtagtacat ctgcctgctt gatgaagcaa gatctaagga ttttgtccca agaccaatgc
     3361 tgattcagag gaagatcgct tgtgggaata tcggatacag gcatgagatc tttatctaag
     3421 aatgtgtcat gctggacaaa gattcctaat tcttcgtcaa ctgggaagta catgttctct
     3481 gcgatatctt tccatttggc tagttcttct tcgctgatag tgatagcagc ttctttttga
     3541 taagttaaat aattttctcg cgtgtaacgc aagacccaag ctgcgatcgt atttgtatac
     3601 caattgttat tgatattgtt ctcatattcg tttgggccgg tcacaccgtg gatcatgtat
     3661 ttatttgctc gttttgaaaa atggacacgg tctgcccaga agcgtgcgat ttctgctaaa
     3721 acttccagtc cttcattttt caaatattcc gtatcgccgg tgtaattcgt ataattgtag
     3781 attgcatagg caatcgcgcc atttcggtga atctcctcga atgtgatttc ccactcgtta
     3841 tggcattcga caccagtgaa ggtgaccata ggataaagcg cgccttttaa tccttgttgc
     3901 tgagcgttgt ggattgcttg cggcagttga ttatgccgat atttcagcag attcttagtt
     3961 acttcaggtt tggctaaagc aagataaaga gggacagcat acgcttctgt atcccagtaa
     4021 gtggctccgc cgtatttttc tcctgtgaat cctttggggc cgatattcaa tcgttcatct
     4081 tcgccgtagt aagtagagaa tagttggaac aaattgaatc gaataccttg ttgtgcttct
     4141 tcgtctcctt cgatgaccac atcggcaagc ttccatcgtt ctgcccaagc cgctgtttgc
     4201 tcttcttttg cttggatata attgtttgtc tggttcgtta attctgagac agctttttct
     4261 gcttgctgcg cttctggaat atctctacta gtaagaacga taatttcttt ttccactgac
     4321 catgtctcac cttgagataa ttctgcagac aagcaaccat gtaatgctaa agtatcttcg
     4381 tgatagtcag ggatgactga ttttccatta acgaaatggc gcattgctgc agtaacagta
     4441 aattgttcaa tcccaaagtt gtttggaata gttttagtcg tcaaatagcc gatttcttct
     4501 atagttccac gatcaatctc attccaaaaa tgttcatcat agttgctgtc ttcgttttga
     4561 acatttccat ccaattttga tacgagtttg actgttccgc taccttcaag catttctata
     4621 gttaaataga tataagctga ttcttttttg acaatgctca gaaaacgttc gaaggaaatg
     4681 cgtactttat tagtagaagt agtgattgtg aatgtacgag ataatacacc atttttcata
     4741 tctaattccc agtaaaagtc ttctggttcc aaagaagcca agtcgatcag ctgatcgttg
     4801 atcgaaatat ctatcgcgat aaagttcaag gcgttgatca cttttccaaa gtattctgga
     4861 taaccatttt tccaccagcc gacacgtgtc ttatcgggat accagacacc cgccaaataa
     4921 gtaccttgat ggtggtcccc tgagtaagtt tcttcaaagt tcccgcgcat ccccatataa
     4981 ccgtttccaa tacttgttaa agattcttgc agtcgaagat tttttttatc taattgtgtc
     5041 gtgctgattt tccatggatc aatagcaaat agtcgtttga tctgtttcat ttttctcctc
     5101 ctaatgagct gttttatacg ttcataataa acgcaaccga ttgcacaagt caatagacaa
     5161 aagaaaatat ttcatagaaa aaagcttaaa aaaataaatt tttaggagaa aattatagaa
     5221 gggaacacac gtattatagg ttttatagaa tggtatagac agaaaaataa aaaataaagc
     5281 atttacaaaa aagaaaacgc acgctataat acgtttaacg aaaccgattg cgtaaaggag
     5341 ctaaacggaa tgaaaaagat atttagtttt gaattttggc aaaaatttgg aaaggcttta
     5401 atggttgtgg tagcagttat gccggcagct gggttgatga tcagtatagg gaaatcaatt
     5461 cccttgatca atcctgattg gacgccgctt gtcacaacgg gcggagtaat tgaaaatatt
     5521 ggttgggcaa ttattggtaa tcttcatatt ttatttgctt tagcaatagg aggaagttgg
     5581 gcaaaagaaa gagcaggcgg agcatttgca gctgggatct catttatttt aatcaatcgg
     5641 atcactgggg ctatctttgg tgtcacaagt gacatgcttg cgaatgaaga tgcatttacc
     5701 cacactctgt tcggtacgaa gatcatggtt aaaggatttt ttacaagtgt attggaagca
     5761 ccagctttga atatgggagt attcgtaggg atcatcgctg gatttgtcgg ggcaatggct
     5821 tataataaat actataatta tagaaaattg ccggatgctt tgtcattttt caatggaaag
     5881 cgctttgttc cgtttgttgt tatcttatgg tcaacaatcg tggcattgat tcttgctgtg
     5941 gtgtggccaa acgtacaggc tggaatcaat aattttggtt tatggattgc agaatcacaa
     6001 gaaagcgcac cgattctagc accgttttta tttggaacat tagaacgttt actattgcca
     6061 tttggattgc atcatatgct gacgattcct atcaactata cacagcttgg cggatcgtat
     6121 gagattttat caggcgcaca ggcggggaca caagtatttg ggcaagatcc gttatggttg
     6181 gcttgggcca ctgatttagt gaatcttaaa ggtgcagggg atatgtcgca atatgagttt
     6241 gtgttgcata actggacgcc ggctcgtttc aaagtaggac aaatgattgg ttcttcaggt
     6301 atcttgatgg gaatggcttt tgcgatgtat cgtaatgtcg atcctgataa gaaagcaaga
     6361 tataaatcaa tgtatttttc agcagcgttg gctgtctttc taacaggtgt aacagaacca
     6421 ttggagttca tgtttatgtt tgctgctgtg ccattatatg tcatttattc agtgatccaa
     6481 ggggctgcat ttgcgatggc agatatcttg ccattgcggg tccattcatt tgggaatatc
     6541 gaattgctga ctcggacacc gttagctatc aaagcgggtc taggtggaga tcttatcaac
     6601 ttcgtgttgt gtgtgatcgc ttttggtgtt gttacgtatt tcttagcaaa tttcttgatc
     6661 aaaaaattca actttgctac accgggacgt aacggaaatt acgacaatga cagtgaagaa
     6721 accgttgtat ctaacagcgg aacaggaaca gcagatcagc aagtggtcca gatcattcat
     6781 ttgctgggag gaaaagaaaa tatcaaagat gtcgatgctt gtatgactcg gttacgcgta
     6841 agtgtaaatg atcgagaaaa agtagggact gaagaagcct ggaaacgagc aggggcgatg
     6901 ggattgatcg tcaaagacaa tggagtccaa gcagtttatg gaccaaaagc agatgtcttg
     6961 aaatcagata tcgaagatct gctgcaatca ggagcagaga ttccagagcc agaaatggtt
     7021 tctacaggaa atggacaagc aggtagtaag caatacttag gtatagaaca agaactagtt
     7081 tccgcagctt ctggagaagt gattccttta tctgaagtaa aagatccggt attctcacaa
     7141 aaaatgatgg gtgacggctt tgctgttatt ccaacatcaa gagaagttgt tgcgccgatc
     7201 gctggaaaag tgacaagtat tttccctagc aaacatgcaa ttgggctgga aacgaaagat
     7261 ggtatcgaag ttttgatcca tatgggaatc gatacagtcc aaatgaatca gccagctttt
     7321 gagattgctg tcaaagaagg ccaagaagta ggaacaggaa cgaaattagc tgagatggat
     7381 cttgatgtga tccaaaacga agaaaaagat ccaacgatca tgatcgtatt tacaaacgat
     7441 aaagtagaag aagtagtgat caagcaatta ggtacagtga cagctggtaa agtgatcggc
     7501 tccattaaaa tgtaaggaga ggttgtgaaa aagctgtcct gcatcaagtc ataagaacag
     7561 ccaaacaaaa atagctcctc ctatttttcg tttggttggg cttattaccg agaagcagca
     7621 gtttgaatac tgtttagtgg agaggtcgtg aaaaagctgc cctgcaccaa gtgataaaaa
     7681 cagccaaaca aaaaatagct cctggtccat tgagcttatt actgaggagt tagcaggaga
     7741 acactatttg ctggagaggt tgtgacaaaa attttgtcac aacccttttg tttcgaaatg
     7801 aagacaagga gaaaaccaat gaaattcatg acactcaaca cgcatagctg gcttgagcca
     7861 gaaccagaga aaaaattgca agaattagca gataagatcc tgttagaaga ttatgaagtg
     7921 attgctttac aagaaatcaa ccaattactg gaatctgaag aagtagaacc tgcgaagcta
     7981 atgaagttct gtccggtaaa gaatcaagta cctatccgta aagataattt tgcctaccgt
     8041 ttagttcagc ttttaaagga acacggaaaa gagtattttt ggagttggga aatgagccac
     8101 atcggttatg ataaatatga agaaggaaat gcactgctta cgaaaaaaac attggaaagt
     8161 caggtcctta ccgtctccca atttcaaaag aaagaagatt atcagacacg caagatcttg
     8221 attgggaaaa caaagataga tgatcaggac atttttgtct cgagctgcca cttttcttgg
     8281 tggacagata aaaaaagtgg cttttatttt gagtggaaaa acttggaaaa ctactttttg
     8341 gaaactcgcg taccactttt tttcctaggt gatttcaata atccagtaga ctctcaaggt
     8401 tattatactg taagagaaag ttgccttctt cttcaagatt cgtatgttgt cgcgaatgaa
     8461 aaaggaaaag cagctactgt ggaaaagaaa atagatggtt gggaacaaaa tacagaaaaa
     8521 ttgcgtatag attttatttt cgtccctgaa ggaatgcagg ttaaaaaata tcaacggatt
     8581 tttgatggga tcgatagtcc aatcatcagt gatcactacg gggtggaaat cgaattagat
     8641 gtgaacgaat aaaaaatata acatataaca taaaaaaact gattaaaata aaactctcca
     8701 aacgattata ccgtttggag agttttttga tcggatgtat ttatcccttt tgcctaagca
     8761 cgtaaaagct tgtatctatg ataaataaat aaacatagca aggtaaacca gccaatcaaa
     8821 gtaagagaaa gaatgaaata gaaatagatt ggcgtttgat aatgaagcgt gagctcgttg
     8881 attccttttt gctgcttcac gattggggtc ccgatatctg ttacttcata atcagtcaat
     8941 gtttttccat tttgctgtag cacagtccga tcgtagacga taacagggat atgaacagtt
     9001 ttgtccccag tgtttttcca cttgattgtc aaaagaccgc ctggatgtgt ttttataaaa
     9061 gttgtttcgt tttctagtat ttcttcgtta taccggtcgt atttattgtc ttttgtctcc
     9121 ttatagattg gcagatagtc aggtgtggtt ttttggaaaa cgaacaagct ggtagacata
     9181 tctttgctga agaatgtttt ccttgcttct tccggcgtat cgaatagata agtatgtcga
     9241 ctgacaaatg tttcattttc ccacttttcc agatgctggt ataagttctg gatcgtctgt
     9301 gctgaaaaaa taagaatgcc aaatatactt aaaagacgga accacttttg ttcagaccac
     9361 tggttcagtg aaagggcaag gtaaagtaga agcaatagcg taaatggcac gaaaaatcga
     9421 aatggaaact ggatcaaact aataaaaggc acattttttt cgatcaatgt cgtccatgga
     9481 atcaagctag tagaaagaaa aaagaaaaca agacttgtgt acaacacgat tttttggaat
     9541 aaggtgttct tttgttgaaa gaaaataaaa agcaaaaaac ctgataatat caatatagga
     9601 aaggccttag ggtaatggag ccaataactg ccatccagat ctatcgcctt ttgactcatt
     9661 tctctgttaa caaagggagg aagaatctta ttcccactgt aaacaatagc taatcctgcc
     9721 caaatatttg ctgtcagaac aaaataaagc aagaccgact taatcagttg aagcagtgct
     9781 tttccttttt cagtctggtt aaaaaagtaa taaagataaa aagggatata catcaaagct
     9841 aacatgatcg aagacagtaa atggatctgt gtcataagtc ccactgagac agcgactttg
     9901 ataacaggga actttttatg aaggacgaaa tcagcaatag ggatcataca taaaggataa
     9961 aaacaagtcc cccagctagt gaatccttgg ttgatagacc agtattgaat agaatacgta
    10021 ctcatgaata aaacggctac tggtacactt acccaatttc gaacattgac atatctcagt
    10081 aaaatcagca tagaaaatcc tgaaataaga taaagaatga aatttgaaag aatctgataa
    10141 tgaaaccagc tacctgcaag caacacgatc aatccttgta aataagcgaa aaatgggccg
    10201 taaacggcat tcacgatcct gccactttgc tggaatccat aaagggaagg aaaataatgg
    10261 aaatccccat atttaatttg ttgcgcggca tcatagaacc gattgtaatg gaatggggca
    10321 tcagcaccca aaattagttt atgtgtgaat aaatgaggca tgagaaatgc caaagcaatg
    10381 aaaaaaattg ctaaaaatgg gccatatttt ttcaattgtc gaactccttt gcaaacttta
    10441 gataaattca tcatatatcc tatgcgagag gtaggcaaga aattagaaaa ctcatgaaaa
    10501 tatttatgaa ttttacaaga aaaacataag tgaaagttct aaggagagag gatgcgacat
    10561 aagttttgtt gcatctcctt gttttgaaac agaatttttc ttaatactcg aaacaaagta
    10621 gctttttcat gacctctcgg taaacggtgt tcaatcagct gcttctcggt aataagctca
    10681 ctagcctaga aaatatgaga agctattttc tgactagcgc tcttattact caaagcataa
    10741 cgcagttttc acaacctctt ccttaaacgg tgttcaaaag cttgcatctg cggtaataag
    10801 cccaacccaa caaaaaatat gagtagctat ttttttgggc tgtacttatt acttgatgca
    10861 ggacagcttt ttcacaacct catcggcttt cattttctat ttttttcctg aacccatata
    10921 ataaaagaaa gaacaaaaaa acttgcaagg gaggacacat gtgaagcaga gtaagcaaaa
    10981 acagtctcgt cgacttttgt ttcgtttgtt tttattaata tgtatcgtcg tagggttact
    11041 gatcgcttta tatccatttt atgtggattc tttaaatagt tttattgatc aaaaacgtat
    11101 cgaacaagta caaaaacgga ccgcagctga aattgaagtg cagcgaaaga aaatggagga
    11161 gaaaaaccaa cgattaactg accaaggatt caatcctggt actgatcctt tcaatgaaaa
    11221 gaacagaaat gaaagtacaa catccagtca attagaggaa tggctgatcg gaagtatcaa
    11281 tatacctaag atccaattaa atatttcact ttatgatcgg ctcaatggta ggatccttga
    11341 aaatggagca ggcgtcttgc aaggaacctc tttcccgctt ggtggaaatt cgacgcattc
    11401 agtgatctct gctcacagcg gtttacctaa tcgacggtta tttaccgaat tagatcgttt
    11461 agaaaacgga gacacgttta ttttgactgt tttgggggag aaattggctt atcaagttaa
    11521 aaatatacaa gtggtactgc cagatgacac gtctgttcta acgatttcag aaggagaaga
    11581 cttagttacc ttgcttacat gtacgccgta tatgatcaac acgcatcgtt tgctagtgac
    11641 aggacatcgg attccttata acgaatcggt aaaaaaagaa gaaggaaaag gaaatcagga
    11701 aagaaccatg cggcaatggt ttattcttgc aggaacgata atagctgtag tcatactgtt
    11761 agtgtttatc ggacgactaa tctatcagta tagattaagt aaaaaagtgc ttgatttttc
    11821 atttattatt agtgattcgt cagggaaacc ggtaaatggt agttcattca ttttgaaaca
    11881 taagaaaaaa acacttacta gaaatggtgt gccgttttca gtacaaagcg accatcatgg
    11941 gaaagtcaag ctggatcaat tgcctggtgg aacttatcga atcgtttcag ctgatgatcc
    12001 taaagttgct gtttcttttg gtattagaaa agtaaaacaa gagaagatat acttttttga
    12061 ggggcgaaaa cttgtaaaag agcttcaaaa aaatggtttt tattttaaaa tcaatgaata
    12121 agtaaggaga gaaaaaatga tccgcacaac aacaaaattg tctgccaaag aactggaatc
    12181 tgttctatcg atttggctgc atgctaactt ggattctcat gaattcattc catcaactta
    12241 ttgggagaat caagtagaat ctgttaggaa agctttacca gatgcagtga tttacttagc
    12301 agaaatagat caagaaatta ttggttttgc tggggtgatt gatcagttta tcgcaggaat
    12361 ctttgtgaaa agggaatacc ggcaacaaag aatcggtacg caactaatga aaaaaataaa
    12421 agaaaccaat aaaacactga ctttgacagt ctatcaaaaa aatcaatcag ctattcattt
    12481 ctatctttca caaggtttta caattcaaac acagcaaaaa gatgaagcag taaatgagga
    12541 ggaacttgtg atgaaatgga atcaaaacgc tgatttttga tgaacagaat aacgaaataa
    12601 tgctcccagc ctaatttttt gctgggagca ttatttcatt ttgaagtcac taatttaact
    12661 gtgtgaatat gcagttgtta caatctttga taaacggtgt tcaatcagct gaccctcggt
    12721 attagctcaa aaaacggcaa aatatgagga actatttttc catttgtttc tctaatactc
    12781 aggtcaaaac gctgatttca caacctcttg ataaacggtg ttcaaacggc tgcttctcgg
    12841 taataagccc actagcctag aaaatatgaa aagctatttt ctaactagcg ttcttattac
    12901 tcaaagcata acgctgattt cacaacctct tgataaacgg tgttcaatca gctgaccctc
    12961 ggtattagct caaaaaacgg caaaacatga ggaactattt ttccgtttct ttctctaata
    13021 ctcaggtcag aacgctgatt tcacaacctc tctttcacaa ccttatttta ctcgtttata
    13081 attttcatcg aagtattttc cgctacggat atcatctggc attccatcgt atggttcttc
    13141 gctaataaca tcttcattgt ctatacctgc atcacccata taagtgatcg tagcaaattc
    13201 aggaacaagt aattttggat aagtttcata tttttcacga gctgcttcca tttctgcgat
    13261 atgttcgttt tggaaagtaa cagtgatttc tggatgttct tcaacccatt tagggaaaaa
    13321 gatcattccg tgaagtttct tttcatttgg cataaagtcc aatgccacgt ctgtccctgt
    13381 tggctctgtc caagtgattt tgaatacgcc gtccgtcaat ttaacgatat cagcttcttg
    13441 gtcttttacc caacggcctt tgaccattcc gccatggata cggtaatcaa cagtatggtc
    13501 atttttggca taccattcat attcccaacc attgtcatac gtatagataa agtgagttcc
    13561 aataaaatct tccaattctt taaattcttt catgtttaag catctcctta taaattaaaa
    13621 catgtaataa actacatgtt ttcgattact catttatgat agaatggatt taaagatttg
    13681 tcaaggaggg aaaactgatg gcaagaaaaa atacacttcg atatattttg ttagggttat
    13741 taagtaagaa gaagatgaca ggatatgaat tgaatcaatc gttcaaaaat gaaataggag
    13801 aattttggca agcaaaacat agtcagattt atcctgaact tgcaaaaatg gaagagcaag
    13861 gaatcattca gcatcaagta gagatcacag gtgaaaaatt ggaaaagaaa gtttatgaat
    13921 taacagaaga agggaaggaa caattggcag agtggattca tacacccacc aatgaactac
    13981 cggttaatcg agacgaattt gttctgaaat tgtatttcgt caaagacgtg aatgatccgg
    14041 cattagtaga aattattcag cagcagcgtg accttcatga agaaaaacga cttcatctat
    14101 tagagcgaaa aaaaacgatc tttcctactg aaaaagaaaa acatgaaaat tacggacact
    14161 acctgatttt agcgcatgcg atcaatcggg aaacagaata tgcaaattgg ctaacagatg
    14221 tattagaaga aagtaaccga aaaaaataaa aaaatatccg aacagtgatg gagaattcct
    14281 ttctatatga aagaaacgca gatcaccgtt cggatattct tatgttcaaa caggcctttt
    14341 ttcaagtatt tatgtagcat ctacttatta aaaagcttgg tacttaggag attagtggcg
    14401 tttagagttc ttttaacaac tgttcgtagt cagctttttc atcgacagga aggagcttgt
    14461 aagttaattc gtctgtctcg ttgtcatgaa tgatgtacat cttagcattc tcttctttga
    14521 agttaccgat ttgtaaaatc gtatagcgtt cgtcgatcaa ttcgatatct ttgattgctt
    14581 ttgaaaaaac tttacggaca tggtgcattt tcttttggcg gatttctgcc ttttctgcag
    14641 cttttgacaa agtaaatccg tcatctaaat attttttgat cagttctact tttatgactg
    14701 taggtaaacg atattttcta ggacaattcg catcagcttc gatcgaacga atgaatccct
    14761 tttgttccca ataccttaat tgacgtggag acacacctac gatatcactt aattcactga
    14821 tgccaacaac caattgatca ttttccaaca aacgtttaag ggaagcccca accatttttg
    14881 tcactccttt tgtcctcttg cttcccttaa ttatatccgt attaacttat gtgtcaagta
    14941 ggttgacaaa tagcgaattt aggactaaag tattctagta gtttcagaaa aaattcagaa
    15001 agaaggagaa atatgagtaa caaccaaccc gtagatattt acggaaaacc atataatcgg
    15061 acacttttag ttgttgtctt attaattggt acattttgta ccgttttgaa tcagacctta
    15121 ttgacgacag cttttcctac attgatgaaa gctttcgata tttcagcgtc tgatgtgcaa
    15181 tggttaacga ctggtttctt gctagtcaac gggatcatga tcccaataac agcatggctg
    15241 atcaataaat tcagttcaag aaatttatat ctttctgcca tgacgatctt tttgatcgga
    15301 acgattactt gctttacagc acctaatttt agtacattac taatcggtcg tttgatccaa
    15361 gcttgcggtg tgggagtatc gatgccatta ttgcaaaata tcatgctatc tattttcccg
    15421 ccagaaaaaa gaggatcagc aatggggatg tcagggatcg ttattggact tgctcctgct
    15481 ttaggcccta cattatctgg ttatatcatt gataactacc attggcgtga cttgtttggt
    15541 atggtactgc caatcgttgt tcttgtcttg gttttagcat tcttcctaat gaaaagtgtt
    15601 attccattgt caaatcctag cattgatatt ttatccgcta ttttatcaac gattggattc
    15661 ggtagtttac tttacggatt ttctagtgta ggagatgacg gctggggaag cttgaaagta
    15721 attggtttct tagttgttgg tgtgattttc atcgccttgt tctcatggcg tcaattacat
    15781 ttggaacatc cattcttgga acttcgtgta ttcaaatcat ctacattcac gattgcagct
    15841 gttttagctg gtgtaacaaa tatggcgatg gtaggggcag aaatggtcgt accattgtat
    15901 attcaaaata ttcgtggaga atcagcattc cattctggct tgatgttgtt gccgggtgca
    15961 ttgatcatgg gggctatgat gcctgtgacg ggtgcgattt tcgataaata cggcgcgaaa
    16021 cgtttagcca tcacagggat gttcattttg acagcggcaa ctgcaccatt tgctttcttg
    16081 acaaaaacga ctccagtttt atatatcgtg atcctttatg cgatccgtat gtttgggatc
    16141 tccatggtca tgatgccggt aacgacttct ggtatgaatg cattgccaaa taacttgatc
    16201 agtcatggga cagcggtaaa taatacattc cgtcaaatcg caagctcgat tggtacagct
    16261 attttgatca gtgtgttgac gaatgtgaca aaagcaaata tcccaagcaa atctgtattg
    16321 gataatacgc cattagctta taaagatcag gcaatcaatg ccacactgtc aggttaccat
    16381 gccgcgttta tcgtagcgat catctttggg attcttggct ttattattgc ctttttattg
    16441 aagaaaaaag aacctgttct aatgaaagcg ggggataatg catgattatt ggagttttga
    16501 ttgtcagctt gcttgctttt gctttaacga acgtgttcgc taaaaaaaca tggcaaacct
    16561 ttttatcttt gattttcgga ctaatcttcg tcgcttcact aagtttgatc gtcgctaatt
    16621 tgtccaatca ctttggaatg gaaaaagtaa cggaaacaaa aacagaaaaa attgtttcca
    16681 gtgctgattc ccaaggagca gatatgcttt tgtataaagc tttagggaat ggaaaagaaa
    16741 aagtgtatct ttaccgaaca aatgaaaaac aagaaaaacc aaaagcaact ggtacggata
    16801 acgaaacaaa taaagtagaa aagacagacg gcgatgctga aaaagtgacg aaaactactt
    16861 attgggaata caaaaacgac atgtataaat tctggttcaa tattgctgat aacaatcacg
    16921 aatatgacag ccgagtgaat acattcaaaa tcccagagac atgggtagaa ttaagtacgg
    16981 accaagcagc caagttagct gaattagtga agaaacagca aagtacgatg gaaagtgaag
    17041 caaaagctta tgttcaagac ggtatggtaa aagcaatgac tgaaaatcca aaaatgagca
    17101 aagcagagca agaacaacgt acgaaagcct tagctgctga attccaacaa caagcattcg
    17161 caaaattagt gaaagaagca aaaggcgaat aatattttga acaccatgta
//
LOCUS       DACDXE010000066        17150 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.066, whole genome shotgun sequence.
ACCESSION   DACDXE010000066 DACDXE010000000
VERSION     DACDXE010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.066"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            138..1475
                     /locus_tag="IWM23_002178"
     CDS             138..1475
                     /locus_tag="IWM23_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAP9884789.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="IWM23_002179"
     CDS             1523..3652
                     /locus_tag="IWM23_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAP9884790.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="IWM23_002180"
     CDS             3627..4316
                     /locus_tag="IWM23_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884791.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="IWM23_002181"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="IWM23_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="IWM23_002182"
     CDS             complement(5546..5740)
                     /locus_tag="IWM23_002182"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884792.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="IWM23_002183"
     CDS             complement(5790..6230)
                     /locus_tag="IWM23_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAP9884793.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="IWM23_002184"
     CDS             complement(6234..7079)
                     /locus_tag="IWM23_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAP9884794.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="IWM23_002185"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="IWM23_002185"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAP9884795.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="IWM23_002186"
     CDS             complement(8703..9650)
                     /locus_tag="IWM23_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9884796.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="IWM23_002187"
     CDS             complement(9873..10223)
                     /locus_tag="IWM23_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAP9884797.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="IWM23_002188"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="IWM23_002188"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAP9884798.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="IWM23_002189"
     CDS             11646..11966
                     /locus_tag="IWM23_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9884799.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="IWM23_002190"
     CDS             11988..12452
                     /locus_tag="IWM23_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9884800.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="IWM23_002191"
     CDS             12657..13262
                     /locus_tag="IWM23_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAP9884801.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="IWM23_002192"
     CDS             complement(13301..14590)
                     /locus_tag="IWM23_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAP9884802.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="IWM23_002193"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="IWM23_002193"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAP9884803.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16250
                     /locus_tag="IWM23_002194"
                     /pseudo
     CDS             15700..16250
                     /locus_tag="IWM23_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
     gene            16321..>17150
                     /locus_tag="IWM23_002195"
     CDS             16321..>17150
                     /locus_tag="IWM23_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HAP9884804.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT         5459 a         3105 c         3467 g         5119 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttgaagaga gtgtaaaata ttttgtgtaa atagaaaaaa
    16261 ggaagtccct tctgtagaat agagttacca caacacattc acagaaaaga ggacttccat
    16321 atgaacgatt ttactacaga aattatggaa atactaatca ataaaggtga tttggatgat
    16381 ttgtttcgtc gtcacctaga attggctatt aacgctttat tacaagctga attaacggta
    16441 tttcttgact atgaaaagta tgatagagct ggatttaatt caggtaattc ccgtaatggg
    16501 aattattcgc gctcttttaa aacagagtac ggtgaattga atttagtaat acctagagat
    16561 aggaatggag acttttctca acaaacttta cctgcttata aaagaaccaa tgactcttta
    16621 gaaaccacta ttattcagct agttcaaaaa ggaattacta tgtccgaaat ctctgatcta
    16681 atcgaaaaaa tgtatggtca ttattacaaa ccacaaacta tttcaaatat gagtaaaatt
    16741 gtgtctgaag atgtttcggc atttaaagaa agaactttag aagctaaata ctcagtcatt
    16801 tttatggatg ctactcatat tcccttaaaa agacaaaccg tatcaaaaga agctgtctat
    16861 atagtaattg gtatcaggtt ggatggaact aaagaggttc taggatttac tatagctcca
    16921 actgaatctg cttatgtttg gaaagagatt cttcaagatc taaaagaccg tggtttagaa
    16981 gaggttttgt tagtcgtaac agatggttta agtggtattg acgatagtat tcatagtgtt
    17041 tatccaaatg ctcaatttca acaatgttgt gtccatatat ctagaaatat cgctcataag
    17101 gttcgtgtta gtgatcgaca agaaatctgt agcgatttca aattagttta
//
LOCUS       DACDXE010000067        16590 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.067, whole genome shotgun sequence.
ACCESSION   DACDXE010000067 DACDXE010000000
VERSION     DACDXE010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.067"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(79..786)
                     /locus_tag="IWM23_002196"
     CDS             complement(79..786)
                     /locus_tag="IWM23_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAP9884805.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(865..1203)
                     /locus_tag="IWM23_002197"
     CDS             complement(865..1203)
                     /locus_tag="IWM23_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAP9884806.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1314..2276)
                     /locus_tag="IWM23_002198"
     CDS             complement(1314..2276)
                     /locus_tag="IWM23_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAP9884807.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2291..3622)
                     /locus_tag="IWM23_002199"
     CDS             complement(2291..3622)
                     /locus_tag="IWM23_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAP9884808.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3630..4310)
                     /locus_tag="IWM23_002200"
     CDS             complement(3630..4310)
                     /locus_tag="IWM23_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAP9884809.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4529..5008)
                     /locus_tag="IWM23_002201"
     CDS             complement(4529..5008)
                     /locus_tag="IWM23_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAP9884810.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5135..6814)
                     /locus_tag="IWM23_002202"
     CDS             complement(5135..6814)
                     /locus_tag="IWM23_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAP9884811.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7362..8864
                     /locus_tag="IWM23_002203"
     CDS             7362..8864
                     /locus_tag="IWM23_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9884812.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8877..9539
                     /locus_tag="IWM23_002204"
     CDS             8877..9539
                     /locus_tag="IWM23_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884813.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9640..9843)
                     /locus_tag="IWM23_002205"
     CDS             complement(9640..9843)
                     /locus_tag="IWM23_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAP9884814.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWM23_002206"
     CDS             complement(9954..11138)
                     /gene="iadA"
                     /locus_tag="IWM23_002206"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAP9884815.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWM23_002207"
     CDS             complement(11295..13034)
                     /gene="yfcC"
                     /locus_tag="IWM23_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9884816.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13807..14421)
                     /locus_tag="IWM23_002208"
     CDS             complement(13807..14421)
                     /locus_tag="IWM23_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9884817.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15027..15404)
                     /locus_tag="IWM23_002209"
     CDS             complement(15027..15404)
                     /locus_tag="IWM23_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884818.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWM23_002210"
     CDS             complement(15704..16585)
                     /gene="galU"
                     /locus_tag="IWM23_002210"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAP9884819.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4808 a         3574 c         2833 g         5375 t
ORIGIN      
        1 ttccaaataa atggtattca atcagctaac tctcggtatt agctcagatc aaagcgctga
       61 tttcacaact tctttatttt aatctcttcg gttgccgccg aacaataaga tcaagcgcaa
      121 cagctgcagg aatgtcgaaa gcgctgctgc aacataagtc agtgctgctg caaataatac
      181 gtgtcttgcc atcggtactt cttcttccgt cagtagtccg ccttcactca aaatttggat
      241 tgctcgccga gacgcgttaa attcgactgg taatgtcacc aattggaaaa gtaatgctaa
      301 agaaaaagcg aaaatcccga tattgattaa cgtagagttc cagctaagca atacacctac
      361 cataatcaag gggaacgaaa tcatcgaacc aaagttagct gctggaacaa gtgctgcacg
      421 aatcttcaat gggacataac ccgaagcatc ttgtaccgca tgtccgcatt catgagctgc
      481 tacacctatc gcagcaactg aagtagactg tgcagttgct tgagacaaac tcaacatctt
      541 attacctgaa ttgtagttat ctgtgagatc tccggcaatt tgttgaacac cgacattatt
      601 tatctgttga ctttgtaata tatattgtgc tgcttgtgta ccagtcacat tgtttttgct
      661 tctcacttga tcgtattttc ggaaagtact attgacgtac gctgacgcaa ttccggaaat
      721 aactaatcca agaatgacca aaagaaatgt cggatctaaa aaaccataca ttccataacc
      781 atacattttc gtttcactcc ttttcgttta ctttatttta tcattagtag taggaaaaga
      841 atgtgaaaag accttgaata ttctttaatt ctttttcaca aattccgatt tcagtttcat
      901 cggtccaaat ccttctacct tgcaatcgat attatgatcg ccttctacta aacggatgtt
      961 cttgactttt gttccttgtt taatggcacc tgacgcacct tttactttca agtctttaat
     1021 cactgtcacg ctatcgccct ctaacagacg gtttccattt gcatctaata ccactaatcc
     1081 gtcttctatg acagtttctt gttcttcttt gttccattcg ttcccgcatt ctgggcaaat
     1141 caacaagcct cgatcttcat atgtgtactc tgagccacat tctgggcaat ttggtaatgt
     1201 catcttcttt ctcctttaaa atctttagtg agcactattg taacggaaaa agaagatctt
     1261 gaaaagatac atttgtgatt tttactacaa aaggcacgtt ttcgaacgaa gtatcattct
     1321 gtttttctgc tcttatacga ttggcatata tcttggatct ctttatagat ttcctctact
     1381 tctgctaaat cttttgcatt cgcaccactt gctaatggat ggcctcctcc atgatggcgt
     1441 ttagccaatt cattgatgat cggcccctta gaacgtaaac gtacgcggta ataccctgaa
     1501 ggctgctgaa caaaaatccc ccaagccagc acttcgtcaa tcactccagg aagtgaaacg
     1561 atcgaagctg tctctgaatc atcgattcca tatttatcaa gaatttcttg cggaaggatt
     1621 attcttcctg caccattttc atccacctgg atattttgat aaagataacc ggaaagttta
     1681 gcgactttca tcggcatttg ttcaatctct ctgttcaaag cacttgcatc aaatggataa
     1741 tttctcaata tcgaagcaat ctctaatgta cgggacgtcg tggagggata caaaaagcgc
     1801 cctgtatctc cgacgattcc tgcataaagt aaccgtgcag ccttaacgtt catctttaaa
     1861 gattcaggaa acatcaaagc aaattctgca atcatctcac tacagctact tgcttttgta
     1921 tttacccaca caagatctcc ataagggtca tcattgggat gatggtcgat tttgatcagt
     1981 ttttctgcca actgatatct ttgatcactg attctgggtg aattagctgt atcagtcacg
     2041 atgactagcg ctccttggta gaagacatct tcaacttctt gcatttctgc taaaaagcga
     2101 agcccttcaa tcgtttcacc tacttgacgt atttcttttt ctgggaaact ttcccgtaat
     2161 agttcggcta atcctacttg tgatcctaat gcatcaggat cagggcgcaa atgcctgtgg
     2221 ataatgattt tatcgtattt ttttattgta tctactattt cttgctgtac tgtcatttca
     2281 ctcctgcttt ctaagaacgt tccattactt gacaaacaac gattgctttg gaaacgatgg
     2341 tgttttcaat aaacacttcg atatccagtt tagcggaacg tctgccgatt tccaatacac
     2401 gtggacgaat gtctaattcg ctttccaatt ggatcagccg caaataatgt aggttcactt
     2461 gttcaatcaa gatatttcgt ttttggttca tcaccattgt tttttgtgcg acatcagaaa
     2521 tgatttcgct aagtacgcca aaagaaatgg ttccaacgcc attgaccatt tgtggtgcta
     2581 ctacgaattt gaacttaggg ttttccaaac gatttccttc acggtcagta tcaatcgtat
     2641 ggatatttcc agatatctga tccgaaatcg tatctgcaat ctgcggttgt cgctggacaa
     2701 gctgcatcgc tttcattaca tcttgccggg taatcaagcc ttccaatgtc aagtcatcgg
     2761 aaacgaccgg catgacttct agcccatccc agatcatttg atggctaaca gaggcaatac
     2821 tcattgtttt ctttacactg attggttctt tcgtcatcac ccgatcgatg atctggtttg
     2881 gcgatttccc taaaacatct tttgccgtaa caatccccat caaacgatga tggcggttga
     2941 cgactggaaa acgcgaatgc tgcgtttctt ctgacaaacg ctgataatca gaaatcgtat
     3001 ctgtctgctg caaataatga gtcttttcag ctggcatata gatatcactg acaagcatga
     3061 tatctttttt gatcaattgg tcgcttaacg ctcggttgat catggttgcg actgtaaatg
     3121 tatcataaga ggtgcgtaag atcggcattt caagatcatc agctagcttt gcaatctcag
     3181 cagtcgtatt gaatcctcct gtgatcaata ctgcggcgcc atcttccaat gctaattttt
     3241 ggacttccgc ccggttgccg acgatcatta gtgatcctgg cgtaatatag cgttccatgg
     3301 catgtttagt catcgcgccg atgacgaatt tattcaagac cttgtctaat cctgccgccc
     3361 cgcctaaaac gtccccttca atgatcctga cgacctcgcc aaatgtcaaa cgttcaatat
     3421 gtttttttaa tttgcgttcg atacggatgg tccctactcg ttgaattgtt gaaactagtc
     3481 cgacattttc tgcatctttg attgcacggt acgctgttcc ttcactgaca ttcaattctt
     3541 ttgcgatgct tcttacagaa attcgattgc caacaggtaa actttcaata tattctaaaa
     3601 tcaaatcatg ttttgtcgcc attttctgcc tacacttcca gcgtttccga gacgctcatt
     3661 atttttcctt cactatcttc cagtaactct acaaaagatt cgggatcttg ttcaatgaca
     3721 gggaaagtat tgtaatggat tggaactact ttcttggcac gaattgtttg cgcagccttt
     3781 gctgcatctt ctggccccat cgtatagtta tcgccaatcg gcaagaaagc gacatcaata
     3841 tcgaattctt ctgcaagcaa tcctagatca ctgaaataag cagtatcccc tgcatgataa
     3901 atcgtttttc cttctgcttg aataataatt ccgctaggct cgcccatata ctgaacgata
     3961 ccgtctactt catagcttga actatgctgt gcatgtacga attttaccac gccgaatgga
     4021 aacgcatgtt ttccaccgat attcataccg aatgaatcta ctcctctgct gttcgcataa
     4081 tcagcaatct caacaatact gataattgtg gcttgatttt cttttgcaat aggaatcata
     4141 tctcctatgt ggtcactatg accatgagta atcagcaacc aatctgtctt tacttcttct
     4201 gcagttaagt ctgtcaatgg atttcctgta ataaacgggt caatcatcac gcgttggcca
     4261 ttttccattt caattgttaa acaggaatgt ccatgatacg taattttcat attttacacc
     4321 tcgcgttttc tctgtatctt ttttattaaa aacgatttca tcacttgcac atcaatgaaa
     4381 aaatatagct gctaaatata ttttcattgg attgatagta cagtatgtac aaagcaattg
     4441 taacagataa tacaggatgc accaagtacc aagcagatat aatctcaagt ttgaggtctc
     4501 tgctctgctt agcttctctt ctcgtccctt aactaattaa atcagcgtaa ataggatgga
     4561 cgatcttttc taaatcggct acttttatcc gaaggcttct cccgatttca ccgcttgaca
     4621 caattacttc ttctttttct tttaaagaac tgtctgctaa aaaacaataa tcaggatgtg
     4681 cgagttttat tccaataggg gtgtttgctc cgtggggata tcctgtatgc attaatacaa
     4741 aatccattcc aggaaaacca actttacgat ctttcgtcac ttgtctcgtt tttttgtaat
     4801 ctaagtgatc gttcaaagga atcaatgcga tgatcacccc agttttgttt cctttcacta
     4861 cgagagtttt acatatcaaa gctggatcaa tccctctttt ttgtagttct tctgtgaaag
     4921 attcttctac cacatgacta aaatcaagtg gttgatagct gatcttttgt tcttctaaat
     4981 aacgttcgac ttttgtttga ctcatcattg attcactccc tttattctat cgtttattat
     5041 aaagcgaggg atgctgtatt caaaaattat ttgtttttcg caaaaaaaga gcaagacgag
     5101 cactctatag agaggttcat cttactcttt ttgtttattt tttatttcga cgttgagtag
     5161 cttccataat gactggcaag atcactgggc ggcgttttgt ttgttcaaat aagtaacggc
     5221 ctaattgatc tcggatgtct tgctttaatt tgctccactc aaaatcattg ctttctaaat
     5281 gtttctcaac gattgtttca atcatattgc tgctttcttt catcagatca cggctagctt
     5341 ttacgtaaac gaatccccgt gaagtaattt gaggttttga aatgattttc ttttctcgcc
     5401 gactgatagt gacaaccgct acgaaaatcc cgtcttctga caagattttt cgatcacgta
     5461 aaacaatgtt tccgatatca cctatgccta atccatcaat cattacattt tctgcacttg
     5521 tagttcctgc aacactcata cgcttgttct tatattccag cacgtctccg cgtccagtaa
     5581 tatagatatt tttgtatggc atccctactg catgagccaa atctgcatga gcagctaatt
     5641 cacgatactc tccttgaact ggcacaaaat atgttggttt catcaaattg atcattaatt
     5701 gcaagtcatt aggattggca tggccagaaa cgcgcatatt ctcagagatc aacttcactg
     5761 ttccaccagc tcgataaatg atatcttccg ttttcgccac gatggtttcc atcgcgatgc
     5821 ttggcgtagt agtgatataa accaaatctc cttcttcaat cttgacatta cggtgtgtac
     5881 cgtttgccat tttttgcaga gacttgatgg gttcacccat tcggccagtt tctaaaatga
     5941 tcagttcttc tggatcatag tttttcatgt ctttcaacgt aacaagcaga tcttcacttg
     6001 gtaattgaag tttttctaaa cgcatcgctg tgcgaataat tcgttcgaaa tcttgaccag
     6061 tcaagacaac ttttcgttgt gctttttctg ctgcatccaa tacttgttgc actcgttgca
     6121 aattacttgc tacacaagcg acgatgatac gcccttccca atatttgatc gtatcaaata
     6181 cttcatctgc tatttgggct tcagaagcta caggctgagg attttctgca tttgccgatt
     6241 cacttagtaa ggctaagact ccttctttgc cgatttccgc caatcgtgca taatctgttt
     6301 gatacattgg gatagccgtt tggtcaaatt taaaatctcc cgtataaacg atactacctt
     6361 cttttgtttt cagattgatc cccactgaat ctggtatcgt atgagtggta cggaaaaagc
     6421 tcactactgt ttcaccaaag tctatttctg tatgctcatc taccacatgg aaatccttaa
     6481 atcctttggc tgcttcatta cgactgacat tcaattttgc taactcgatc gtcaactctg
     6541 tcccgaatac tggtacagaa atttttgaca acaagtaagg aagtgccccg atcgcatctg
     6601 catgtccatg tgttaaaaag acaccagcta cacgatcaat gttttcttct aaatacgtaa
     6661 aatcagggat tacgacgtcg atccccaaca actcattttc aggatatttt agcccacaat
     6721 ctaaaataaa aatatcctcg cccacttctg cgacatacat gttttttcca ttttcacgaa
     6781 cgccgcctaa tgggataatt ttaatggtac tcacaaactt cacctcttct tatgacatcc
     6841 aagtttaaag atgtcgattt tcttctttat ctaaatatat gagccattcc aatgaagcat
     6901 atgcaaaatc actatttctc aacttatgga gaatcagcag gaaaattacc agcacatccg
     6961 cgttctgaca gttggatggg cattttattt tggatatact tcattaacaa aatggatctt
     7021 ttgcgaaacg ctatagccag aatacttata taagtattca gctgttcccc attattatac
     7081 aacaaataaa ctaaatcgct agtatcagca attgttagaa caactatgca cgtaaaaatc
     7141 cctctgtagc atctaggatt tcttttgcta ttcctctttc atttcttctt cgtcggtttg
     7201 aaacatactt ttttcatttt accacatttt ttgcaataac tatagtgact ttttatatat
     7261 acagaacctc atgttgatgg tgtgcaaaca gcataagatt taagctagct ttaagatagg
     7321 ttgtctatta tcaaatgcat ctactaggag ggatgcaaaa tatgaatttt atgaaacgtg
     7381 catggaaaag tacaaaagta aaatggggac gttcattgtt attgtttgcc gtgttcacag
     7441 ccattcttat ttttgtgcta gctggcctca cgattcgaag cgctgcagaa gaagcagcga
     7501 atcaagcaca aaaagaagtt ggagcaacag tgactttaag tgccaatcga gaagcttcat
     7561 ttaaaaaaca ggaacaaact tcttcaagcg gttctaccac tgaagaacgt ccagatccag
     7621 gcagtttttc tcttacacca gtttccttaa cggacgcgga aaaaatagct gaattagata
     7681 atgtaaagag ttattccttt gaaagttccg cctcagctat ggcaaaagat ggaataactg
     7741 ccatcactag ttctgattca agtgaagaca gttcagaaac tactgatgca ggcggtgaac
     7801 ctgctggagg aatgggcggg aatccgcaaa tgactcaagc agactttcaa atcagtggtg
     7861 tgtcagcaac tgcacaaaat agtaattttt ctgatggaac agcaaaaatc acggatggtg
     7921 aaggaataga tgacagcgac aaaggcacaa acaatgtagt cattgattct actctagcag
     7981 aaacaaatga cctggcagtc ggagatactt tcacaatcac gagtactgaa gatgaagata
     8041 ccacttatga aatgacgatc aaaggaattt atgaatccag cgaaacaagc ggcagtatgg
     8101 gtatgaaatt caacttcatg aatccgtcga atacacttta cacttcttac acttttgcaa
     8161 atgaactaaa tgggacatct gacgataaca cgatagattc tgctatttac actctatctg
     8221 atccagataa aatggatgag tttctcaaag aagctgagaa gctgatcgac acagatacat
     8281 ttagcttaca atcaaatgat tccatgtatc agtcaatgtt ggaaccttta aacaacgtgg
     8341 caagtttttc taaaaatgtt gttttactag tggctgtagc cggtattatc atacttactc
     8401 tgatcattat gattaccatt cgggagcgtc ggcatgagtt aggcgtcttg ctatctttag
     8461 gagaatccag aagtaaaatc attctccaat tgtttaccga agtggctatt tgtatgattc
     8521 ttgcattagg agttgcttcg cttagcggaa acgtcgtcgc gaatgccgtg ggacagcagc
     8581 tacttgacca acagacagaa accacgacac aaaaccagca agctcctggc ggtggacaaa
     8641 tgccaggtaa cagacaagaa caaggcggtc aacgcgatgg tcaacaagga accacgaata
     8701 atccatttgc tgtttctgat caagtcagcg aattggaaat caatgtcaaa cctgcacaat
     8761 tgggactatt agccggagta gcatttggaa tcagtctgtt ttctgtcttt ttagcttcta
     8821 tagggatatt aagattaaac ccaagaaaaa tcctattaaa ctaaaggagg aatttgatgt
     8881 tacggacaga acaattggga ttttggtatc aagatccgca agatgcgctt tataaagatg
     8941 tagaactgaa tttcgaagct ggaaaaatgt acgctatttt aggagcaagt ggttctggaa
     9001 aaacgacttt tctttcgctt atttctggac tagatgcacc aaaagaagga aaggtatact
     9061 atcaagatcg ttcactgaac aaaatcgggt taagaagtta tcgtaaaaat gatgtctcga
     9121 ttattttcca agcttacaac ttgcttcctt atttatcggc tctagacaac gtcatagcag
     9181 caatggaaat cactaagacc aagcaagaga attatcgaca agttgcttta gatcgactgg
     9241 ctcgtgtagg aattgatgaa atattagcaa aaaaacaggt gaccaaactt tcgggaggtc
     9301 agcagcagcg agtcgctatc gttcgtgcta tctgctgtga acatcaacta atcgtagcgg
     9361 atgagcctac tggaaatcta gatgaaacaa cttcacaaga tatcgtcaaa cttttccaag
     9421 aaatcgccca tgaacaaaat cgctgtatta tcatcgtgac tcatgagatt gaagtagcaa
     9481 aaagctgtga tgaagtcttt gaactaaaaa ataaaaaatt tcaaaaaatc gaagtttaaa
     9541 aaacaggata atgatcaaat aaaaaatccg aaccatatct gtctcgcaga tatggttcgg
     9601 attttttatt tgcttgattc aattagtaat aagtggtttt tatttttttt cgtcttttcg
     9661 agaaaacttg tctttgactt cgtcgcctaa atttcgagct ttttctttga tgtcagcacc
     9721 tagtgattgt gctttccctt ctgtttcttt gctcttgtcg tctgttgctt tgccgtatgc
     9781 ttcttttcct tcaccgacta ctttatcttt catatcttta gaacgtcctt ttaaatctgc
     9841 cattgtgctt cctcctctta gtttgtttat tgatctacct tcagggtaac atcaacaaca
     9901 gatacagaca aacagaacgc tcgttttcta aaaaagatag ttagagctgt tgattaaaaa
     9961 cttttttcga aagtccccca tactttgact tctttttctt ggaccattat ttctccttta
    10021 gcgataactg tatcaatatc aagcgtttct ttatccaaga tacacagatc tgcatcagca
    10081 ccaatttcga tttttccttt tttatccaat ttcaatatac gtgctggatt actggttata
    10141 gctcgtaagg cgatttccaa agggatactt tctttttgca cagcttcttt gatccctact
    10201 agcaatgcct tggcacttcc tactcctaac cctaggaaat gattgttttc atcaaaataa
    10261 ggcaagcttc cttgtccatc tgaggtcatc gtgaaattat ctaaagaaac atcttcctcg
    10321 atcaatcgtt tcagtgcttt gctgaagcga acttcaccat ctgtcttctc ccaaaaatca
    10381 ggatcttcgc tagcagtgaa gtcaatcgtg ccaccacgtt tggcaaatgc cacgcaagct
    10441 tcaaataatt ctggtgtgcg atttgcatga gtaggcaaaa attgtgtgat cggaatttct
    10501 gtttcttcta cgattcgtgt caatagctct agctttcttt ttcctccgcc taagtggaca
    10561 ttgatgatcc cagcttttcc ggataacatg cctcctacac gagcatctgc tactgcacga
    10621 gcgaactctt caaaacttgg ctgagaagaa cggtgatcgg atactgcgat ttctccgatt
    10681 ccgataattt tatcaattgt caaaaagtct ttgatgatag aatctgttac tgtttttacc
    10741 ggcaatcggt acgaaccttc gtatacataa gtggagattc cttctgcttc cagacctttc
    10801 gctttgctga tcaacgcagt catatctcta ccttctccgt ctgttcccaa acaaccgaca
    10861 actgtcgtta caccagctgt agtcaattgg ctcaatgtga cttctggcgt tcgattttga
    10921 taaccgcctt ctcctcctcc acctaaaata tggaaatgac agtcaatgaa acccggggtt
    10981 agtacttttc cctcacctga aagttcttct acttccactc cttcgtagcc gccttctagc
    11041 tgttcttcta cagccaaaat cttcccaccg gcaatcagta catccatttt tcctaagtaa
    11101 tctggtgcaa acacttcaat ttgtcgaatg attttcatac atttttcctc tttctttaag
    11161 tataactgat gataggatct taaaaaataa tccaaacgat aggatcttta ctcctttgat
    11221 tttaccgtaa aatgacggtt cttggagtcc ttatctactg tttggattac ttttttattt
    11281 acaggtctcc agtactactg cagacctatt gcaatggcaa tcatgatgaa aatagttcct
    11341 aaaacaaaca ggaatccttg catcttgatt tggaatcttg cccattttgc ccattcgatc
    11401 cgtgcaacac ctagcacacc aatcaagctg gctgatacgg gtgtaaaagc gtccataaaa
    11461 cctgaaccaa gctgataagc aagaactgct acttgtctgg atacacctac taaatctgcc
    11521 agtggagcca tgattggcat agtcaaagct gcttgtcctg aatttgatgt aacaaccaag
    11581 ttgaacaaac tttggaagat gtacatcgcc caagcaccaa tgacagctgg aacaccggtc
    11641 aacgccgtcc cgatcccatg caaaatcgta ttcaaagctg aaggaacaga tgcatcagat
    11701 cctcccaaga caagaagaat ccctttggcc atacctacta cgattgctgt accagctaaa
    11761 tcagctgcac cagattggaa agaagaagcg atgtcattga ctttcattcc atctaatttg
    11821 aaaagaacag caatgattcc agcaactaat cccatcacaa agaattgaga agcgatttct
    11881 ggaatgtaat agcctttttg agtcacgccc caaacgatcc agacaaggac aactaacatc
    11941 tctaataaga tcaatttatg cccaagcatg aattttttat tttcatcaga tgtctttttg
    12001 atatgagaac ggaaataatc atctgatttg taagtcaacg aactttctgg ttttttacgg
    12061 attttttctg catatatcat catataacca gcggctaatg cagtaacaac tacccacata
    12121 atcaatcgga atgtcgcacc agacaacacg gggattccag caatcccttg agccaccgct
    12181 acgctaaacg ggctcatcca agaggtcgca tttccgactt gagatgcgac ataagtcacg
    12241 gttactgcta cgatcgagtc atatcctaac gcaatgacaa atggaaccat caccattgag
    12301 aatggaatta cctcttctgc catcccgaat gtagcgccac cgaatgaaaa agcaaagaat
    12361 aatagcggta aagctaaacg ctccagtccc ttagttttac tgataaacgc atagatacct
    12421 gcgtcgatcg ctccggtacg cataataata ccaaaagcac ctccaacaac aaggatcaat
    12481 gcagcgatcc ctacagcaga tccatactta tctccagata ctagtccttc aaaaacaaaa
    12541 tttaagaaac caaagccgcc aaaatcatct gttccccata ttttggctgt tttatgcaat
    12601 tttttagaag tatcatagat attttcgccg tactgatcgt aaagcacatc atctgtcaag
    12661 gagatttcat ctagtttttc ctgcgtaaga tttttttctc cgtcagcaat gactttttcc
    12721 aatccttttt ccggaacatc cagtttttca cgttctgctg gatgatcttg cagttcttct
    12781 aattgatcaa acacatacga cttatccagc tcgtaagcat aacggaagga atcttgacgt
    12841 aaaacagtac gagtacttgt ttcgccattt gcatctttgt attcaatatc ttgcgtgctg
    12901 aactttcctg ctggaacgac aaaagtcagt atccatgcaa agatgactac accaaaaata
    12961 atgacatacg tatggggtgt cgataattta gtgaagtcgc gtttcggttt tcctgaagaa
    13021 tttgttttct tcaaatttat ttcttccttt cttttttcag taattttgat gaataaaaaa
    13081 tggataatca aatgaattat acagtaaaaa tgacatttcc acatgaataa tttattagaa
    13141 aatcactttt ttctattttg ttttttttct cctatgtatg attatttttt tctctttcca
    13201 tttatattaa gtattttatt tctctgtttt tccttaaaat tcttttttta tttttagatt
    13261 agagaaaatc tcgtcctgtt tttttcattt tacaatcatt gaaaaattga atttcattgc
    13321 aaaaaaaata ataaacactt ataaatatcc attatctatc cttcaatttt tgtgtatttt
    13381 atttctatta gaaaaaggta tcctgttgga caattttgtt atatcaggta aacttcattg
    13441 caataatgat aacaaatgta ctataattac tttgtaaaga gttttgggga agactctttc
    13501 atcaacgtgt attaagagac aagaaagaat ccatatggat tctttctagt ctctttttcc
    13561 actacttttt atttttctga cactacatat tcttataact catttctcat aactgtgaaa
    13621 caacttataa aaataacaaa ctttaaacaa aacaagctta aaatcaaaga tgatccatct
    13681 taaaataact aaaacaaaaa aagtagtcgt tgttaatcct cattaataat cgttttcttt
    13741 ttttcaaacg ctccattttt atgtggaaca ttattttttt aaggaacttt gtcaacatgt
    13801 ggataactat tttccacttg tgtcctggac tagctgtttt gctgtaaaat acaaaacttg
    13861 ctgcagcaaa aagatcgttg caagagaggc tgttccgcag atcgctggat ctagatttgg
    13921 caaagtcaag actgcatcat gtaaattggc gtggttgtcc aaaaccaaat cagcaaattc
    13981 aaataatcgc aaaccactgc tatgttgcga tttgatactt cgtgacaaat caaatccagt
    14041 aacaatgatt aatggatgac cattttcttt gatccactga gctagttcga tgattgccgg
    14101 gtttcttcct tcattcgaaa gaagaagaaa aatgtcttct gatttgacag aaagctgatc
    14161 aataattgcc tgtcctgcac cttctagcgt ttcatagaca cctccggctg gattttcaat
    14221 caatatactt ggtacaagat ccggggtttg atcccaaaat tcaaatgcta caccacttag
    14281 tgtgcggcta gcatatatct gcaatctacc accttgatta atttgtttcg cgatccaagt
    14341 gccagcttga agcatcagcg gctcttctat ccttaatttc tcttccaatt ggtgataata
    14401 gtcaaaataa cataaactca tttatctctc ccgcctagct atacttgaca tgaatagaaa
    14461 cagctgccta atctaaggaa gtacataaaa aaacagtagt tagacagtaa cacatgtggt
    14521 ctttgctgtt tcaaacacaa aaaaggcatt gaacgaatac gcccaatacc ttgacatagc
    14581 aaataaaaca acgtgcagac catggaaaat atctacatag gggtagaatt ttcaccctag
    14641 attaggtaca ataaattagg tcgatgcact gcattgacta tggcaaaatg atcggtcagg
    14701 gtgtatgtgt gtgactggtc attccctgat tcttagcgcc aactaagggt cagggagcct
    14761 ttttttattc agctgtttct atctgaattt attgtaagta atctcttatc caagtgcaag
    14821 caagaaaata caaaaaaaca ttggtatgat ttatatatat ataaataaca taaaaaaagg
    14881 aatggcttat cccgatacaa caagcattcc tttaaaaaag attcttttat ataagtgaaa
    14941 aaagcttttt tccaccaatt aaataaatct ctcatctcaa tagtttatcc attattttat
    15001 ctttttttgc gtatacctac taaatattac cgtcttcttc tcgttcttct actttgttcg
    15061 agagaatctg tactagaaaa agtagttgta tgtctacctt ttctgcctat ccgagacttt
    15121 cttttcatat atgggatata aatgattaga tacagtacag caaaaaataa agcaaatacg
    15181 caagcttccc aactacctaa cgccaattca accatcaagg tagcaacccc aacggatgct
    15241 gcataccaga taaaaatcag aaacgtttct ttttttctga ccatgcgaaa catgataaat
    15301 agactaataa gcaatagtgg caacacaaat atccaattgc ttccttccat cagccacttg
    15361 agtatatcaa gcagcgactg tgcaacagcg actaattttc ccatagtgaa cacacacctt
    15421 tttttataat taaaaaattc gcctttttat tctactatga atcttctggc ttggctttca
    15481 tttctcttat gtaatcattt tcttttatac aacttaaaaa aaaattaaac cattagcata
    15541 atattttctg aatctattca cgaaagtctt tttctatctt tcgttttcca cgactcaact
    15601 gatctagagt caaaaaacta aaacgacagt tgcgcaaatg acgcacaagc tgccgcttta
    15661 ataacaaata gcctttcatt ctcagcaggc cacttatttc cagttaactt tctgttctta
    15721 gctttttact caactggata atatatgcac ttaatgaagt cccaatttcc gaatgttgaa
    15781 gtccatattc aatatttgtc ttcatatatc ctaacttatc gcctacgtcg tatctttttc
    15841 ctttaaactc tctcgcatag accgcctctg tctggttaag agtctgaagt gcatccgtta
    15901 gctgtatctc gtttcctgct cctggttctt gagtttctaa tatctcaaat attttaggag
    15961 tcagtagata acgtccgata atggctaaat tacttggtgc ttttcccatt tcaggttttt
    16021 ctacaaaccc ttttactctg caacgaccct tttcgctatc tgttttagga tcaataattc
    16081 cgtatttaga tgtttcattt tctggtacac gcatgacggc aacagcggaa gcttgtgttt
    16141 cttcataatc catcatcaat tgttgagtca atgggatatc atctttcatc aaatcatccc
    16201 ccagcatgac gacaaacggt tcatttccta taaatgcttt tgcttgaagc acggcatccc
    16261 ccagtcctct tggatgagat tgacggatga agtgaagatt gacttgagtc gtttcttcaa
    16321 ctaaagcgag taattctgtt ttttcctttt cccgcaaatt gttttccaat tcgatatttg
    16381 catcaaagtg atcttcaatg ggtctttttt cttttcccgt tacgatcaag atgtcttcga
    16441 ttcccgaatc caatgcttct tccacaataa actggatcgt tggtttatcc acgattggaa
    16501 gcatttcttt tgccatggct tttgttgccg gcaaaaaacg tgttcctagc ccagcggcag
    16561 gaatgactgc ttttttgacc ttcacttgct
//
LOCUS       DACDXE010000068        16346 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.068, whole genome shotgun sequence.
ACCESSION   DACDXE010000068 DACDXE010000000
VERSION     DACDXE010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.068"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(131..787)
                     /locus_tag="IWM23_002211"
     CDS             complement(131..787)
                     /locus_tag="IWM23_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9884820.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            980..1258
                     /locus_tag="IWM23_002212"
     CDS             980..1258
                     /locus_tag="IWM23_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884821.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1277..2485
                     /locus_tag="IWM23_002213"
     CDS             1277..2485
                     /locus_tag="IWM23_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9884822.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(2633..2998)
                     /locus_tag="IWM23_002214"
     CDS             complement(2633..2998)
                     /locus_tag="IWM23_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAP9884823.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(3145..3918)
                     /locus_tag="IWM23_002215"
     CDS             complement(3145..3918)
                     /locus_tag="IWM23_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9884824.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(4092..4952)
                     /locus_tag="IWM23_002216"
     CDS             complement(4092..4952)
                     /locus_tag="IWM23_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAP9884825.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(4952..6370)
                     /locus_tag="IWM23_002217"
     CDS             complement(4952..6370)
                     /locus_tag="IWM23_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884826.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(6427..6876)
                     /locus_tag="IWM23_002218"
     CDS             complement(6427..6876)
                     /locus_tag="IWM23_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9884827.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(7321..7584)
                     /locus_tag="IWM23_002219"
     CDS             complement(7321..7584)
                     /locus_tag="IWM23_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAP9884828.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(7683..8978)
                     /locus_tag="IWM23_002220"
     CDS             complement(7683..8978)
                     /locus_tag="IWM23_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAP9884829.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(8975..10246)
                     /locus_tag="IWM23_002221"
     CDS             complement(8975..10246)
                     /locus_tag="IWM23_002221"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAP9884830.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(10411..11280)
                     /locus_tag="IWM23_002222"
     CDS             complement(10411..11280)
                     /locus_tag="IWM23_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAP9884831.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(11572..13182)
                     /locus_tag="IWM23_002223"
     CDS             complement(11572..13182)
                     /locus_tag="IWM23_002223"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAP9884832.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(14375..15715)
                     /gene="glnA"
                     /locus_tag="IWM23_002224"
     CDS             complement(14375..15715)
                     /gene="glnA"
                     /locus_tag="IWM23_002224"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAP9884833.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(15743..16135)
                     /locus_tag="IWM23_002225"
     CDS             complement(15743..16135)
                     /locus_tag="IWM23_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAP9884834.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         4664 a         3463 c         2780 g         5439 t
ORIGIN      
        1 cttggtaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaac ggcaaaatat
       61 gaggaactat ttttccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
      121 tctttgttca tcaaaagata atattgtctt ctaacaagtc catattcaaa ataacgatat
      181 gatggtcttt aacggtgata ataccatctt ttttcagttg ttttaaaatt ctgtttacac
      241 tgctagcagt tgagatgcca caaaatttgg cgatttcttc attcgtgatc gaaaaatcga
      301 tcagcatccc ttcttctgtc cgcacaccga ataaatcata taattcgtaa agttgcgtac
      361 aaacagctcc cagctttccg tttaatagca tttgctgcat ttttttcatc gaataaagca
      421 gacgatagcg gtaatactct tttacatagt gttgtagctt tgggtcttgg ttgatatttt
      481 gccaaaagcg cacacgattg attcgataaa actctgcata ttctgattca actcgaatat
      541 tatacggcga atcgatgaat tgtgaatatt cgtcttttaa aagagagacg atttcaagat
      601 ctttgatata gcgaaggtta aattctctcc catcatttga aataacactt gcttttaccg
      661 ttccctcttt caagatatac gtatgggaat cttccatccc ttcgaatgtc aaatatttat
      721 gatatccttt gcgtataatc ggaaaatcat ttttatccaa atattccttt aagactctac
      781 tatccaatga tgctccccca tccaaacttt catctaaaaa gatcatacag tactttaaaa
      841 ctttttaaag tccaaaggta ttttactaat gatattaaca tttgttaagt acctttttcc
      901 atcacttatg cgattttttt cctttttttt attcgttata ctgcagaagt aaaatttttt
      961 aagaatagag ggaaaaaaca tgaattactg gatgaacaca atcattaatc gattagaaac
     1021 agcatatcaa acaagatttg atatgaaagc ttctttagtc tttttaaatg atgcgtatca
     1081 aaactcgatc gaattgatca aagcagtgga tgagaatcca acaaacgaat gcgaagaatt
     1141 tttaaatctg tttatgtcta caagagattt atttattcgc caacttgttg atcgctatcc
     1201 atccaattat cacgatgtag aagttcaaat acaaaaatta aaagcatatt cagcttaagg
     1261 ggaatgtata acatacatgg aaaagaaatt atttaataaa tattttattg gaatcacatt
     1321 gatcaatttt attgtctacc tgatttatta tctgttgatg gtcatcatgg cagtcattgc
     1381 gcaagacacc ttacacgcca gcttgagcca agcaggtttt gcttctggga tctatatcat
     1441 cggaacattg ctggcacgct tattcatggg gaaacagtta gaactatttg gtcggaagtt
     1501 taccttaaga ggcggcgcga tcttctatct cctttcaaca ttagcatatc tttatacgcc
     1561 aactatcggg gcattgtatt tagttcgatt cttgaatgga ttcggctacg gtacagtttc
     1621 tacagctaca aatgctatcg ttactgcata tatccctgca tctaaaaaag gggaaggcat
     1681 caattattat ggattaagta ctagtttagc tgccgctgtt gggccatttt taggaatgat
     1741 tttgctgaat atgactgatt ttcgtttcat tatctggttt tcaattgtac tggtcttctt
     1801 tgttacgatt gcctgccttg tctttcctgt aaaaaatatt ccattatctg aagaacaaaa
     1861 aatatctctt cgctcatgga atatcaatag ttttattgaa aagaaagcac ttttcattac
     1921 aggaattgcc tttttgatgg gtctttctta ttcaagtgtt ttatcatttt tatcttctta
     1981 tacaaaagta atccacttag ttgctgctgg gtcatttttc ttcgtcgtat atgctttggt
     2041 catcacattt acacgtccat tgactggaag gattttcgat gtcaaaggag aacaatacgt
     2101 gatgtatcct agctatttat tcttaacagc aggattattt ttactgagtg taacgacgaa
     2161 tagtgtgaca ttgcttatct ctggagcatt agtcggccta ggttatggta cgtttatgtc
     2221 aaatggacag gctgtttgtt taaaaatcgt ccgcgaacat cgaatcagta ttgctttatc
     2281 tacctacttt attggtttag acctaggctt aggcgttggt ccttatttga tggggatgct
     2341 aaaatctctt acttcattta gaggattgta tgtgattgcc ggagtcattc cattgatatg
     2401 tacagcactc tatcttgtta atggccgaaa acatagtcat gaagcagagg gcgaattgat
     2461 tgaatcattg aaaaatgaaa tttgatcttg atgaatagat aaaaaaaacc gttcagatct
     2521 gaaaataatc tgagcggttt tttgagtaaa aatagtttat gattattctt aattaaacat
     2581 ctatttattt ttaaagcttt catttatcga taagaatagt ttaactgttg atttattcca
     2641 ttttccgttc atctggtacg atgccttgac ttagcaacca cttgtggaag aaccattctg
     2701 ccacaccgat caatagagca ggaatcaata ctttcatcca aatatcgact gtatcattca
     2761 taaaatatag atagagccac aagataactg cagctgaacc agcatctgcg atcgtcgctg
     2821 cgatgttccc tactttacgt aagatcaaca gatcccctag cacatagatc atgactacaa
     2881 taattacccc aacaatcgtt gaatccataa agctaacacc gtataaacct gtcagcacga
     2941 cccacagaat cgcccagatc atgattgcct tcactaccaa cgccttcaca tgtttcattt
     3001 tctgcactcc tttctcctgg ataaaaaagt aagattatct aacaagataa ccttttcatt
     3061 attataccat cggctgagag aagcgagcaa agataaggtc ctatctaaaa atgaggaagc
     3121 cacttagaac attcacgtaa taatttatcc gtacagacat tcgatatggt gttcctttac
     3181 tttcgcatac tgttcttttg tcagttcttt acttgtcacc agataatcga tttcatgaaa
     3241 agcaatggct tgatgcatga atttatgtgc aattttttgc tgtgtaacta aaacggcaag
     3301 ccggtctgat tgttttctaa aagctttttt gaccattgcc tctccttcat gagtttctgt
     3361 tactccttgg ctgaaatcta gcccgcgaca actcattatc gctaaatccg cataatatga
     3421 caatgcatcg ttataggctt ttgaaccgtt gattgttgct cgtttcggaa caattgcccc
     3481 tcccaataca gatacttttg ccgttgtata ttcactcaac atagcagctg tgacgatgcc
     3541 attcgtaatg atctgcaaac catcaaatct tgctaggttt ctgactaagt gtaaagaggt
     3601 tgtactggaa tccaagaata agaccatatc atctcgaatc aaagaagaag ctgtatctgc
     3661 aatcttacgt ttttctgttt cttcttctaa tttggtgatc ttcatcaggt aaggcacgtc
     3721 aatttttcga ttgtcaataa gcatggcgcc accgcgaact ctacggatca atccactttt
     3781 ttccagaaaa acaaggtcac gtttaatcgt ggcttcgcta taatttacta actcgatcaa
     3841 atcggtgact cttgcaaatt ggttgttttt tagataattc acaatctcct gctgtctatt
     3901 ctccacttca ttcaacataa aatcacccca tttactttat atagtaacag gaaaaaagag
     3961 gttgtgacag aagtgattgg cttcgagaaa taagaagaaa tttttcgaaa aaactacttc
     4021 tgaacatcgt gtgtggttaa gagggtatga caagactatt gtcataccct cttgaccaca
     4081 catttttatt tttagtataa actagccttg cctactgtgt tcaaaatttc cattttatgt
     4141 ttgactactt cttgtacctt tttattagct gctggataaa ttgcattcgg ctcgtacatc
     4201 gatggatttt cactcaatat ttctctcaaa gcattgaaaa agacgctttt aagatctgag
     4261 ctcaaattca ctttggcaac tccgtatttt actgcttcgc tgacttctgc gtccggatta
     4321 cctgaaccgc catgtaaaac aaatggaaga tcgatttgtt ggttcaattc tttcagtaag
     4381 tcgatattta attttggagt tctgtttttc ggatacaagc catgcgctgt tccaattgct
     4441 acagcaagtg tatcgattcc tgtttctttc acaaaaatct tcgcttgatc aggatctgta
     4501 taaatgattg tttcagcacc tccttcagca gaaccattat tcccaatcgt tccaagctca
     4561 gcctccactg agacatttac cgcatgagct aacgcaacca cttttttcgt aatctgtaca
     4621 ttgtcgtcga atgatttcaa agacgcatcg atcatcacgg aagaatagcc gtttcgaatc
     4681 gcccgtaaga catcttctat cgtaccgcca tgatccaagt ggataacgat cggcagttta
     4741 cttgtataag cataggctct tactgccgca acaaactcat cacttaaata ggcgatttca
     4801 tcaggatgaa tttctaagat tactggtgct tctaatctct ctgcttcttc gatgattgct
     4861 ttcatcatat catagctgca aatattgaag gctggtactg caaattgatt ctctttagcc
     4921 actttcaata actctttcat tgtatataac attacgcttc cacctcattt gttagatttt
     4981 catttttcgt ccacttgtct tgttttttct ctttcactgt ttttttcaat agtccaagca
     5041 taaaggctgt agtgactgta cctgatgcaa ttgccaagac atacataggt aaattgttca
     5101 taacatttgg aattagcatc acccaaatcc ctccatgagg tgccattgaa gtaactccta
     5161 ctgccatgga aatccctgca gcgactgctg aacctgtcat caaactaggt atcacgcgga
     5221 tcgggtcaga taccgcaaac ggtatagcac cttctgtaat atacgaagcg cctaaaatcc
     5281 agcatgattg acctgaagtc cgttcttctt tggtaaattt atgtttgaat aataaagtgg
     5341 ctaaagcaat acctagggga ggtgtcattc ctgcagccat acaagcagca atcggtgcat
     5401 ttactccagc tgacatcaaa ccgatcgaga aagtcgaaat cgatttattg atcggcccac
     5461 ccatatcagc tgccatcatg atcccgataa gtaatccgaa caatagcccg gatgtatttc
     5521 ccaagctatt cagccaattt gtcaaagcat ccaacaccca tgtgatcggt tgttcaatca
     5581 caaataccat caaaagccca ataattaaga tcgataaaaa ggggacgaca atcagctgat
     5641 aaatagcaga aaattctttt tttacgctaa ttttatttgc cagaaaatca cagatatacc
     5701 ctgctagcac gccaccaatc attccgccaa gaaaacctga accacctgta gaggccagta
     5761 atccggcaac aattcctggg gcaaaacctg cttttccagc aatcgatgta gcaatccccg
     5821 cgcctaacgc aggaaccatc atggcaaacg ctgtatctcc tccgatcctt gaaaatgcag
     5881 ctgctaacac attatagtct gctgagttgg gatcagccgc tgtaatccca aaggcaaaac
     5941 tcagtgcgat gatgattccg cctgcgatca caaatggaag catgtaattc acaccattca
     6001 tgaaatgatt atagattgtc ggctttttct catttgcagc tggttctttt tcattagaca
     6061 caacgaatac gccaatatct ctcaatgctt cgtctattac tacttgcggt tctttgattg
     6121 ctcggcttgt agagacacgc tttacttttt tcccggcaaa tcggtccaat tcgatttctt
     6181 tatctactgc aagaatgatt gcatctgcct ctttgatttc ttgttcagat aactgatttt
     6241 ctgtttttac accttgtgtt tcgaattttg cttgatggtt tagatttctt gctgttactt
     6301 ctaatttttc tgccgacata taagtatgag caattccagt cgggcaagca gttacaccaa
     6361 cgattttcat gattattttc ctcctttttc ttcactatcg atactcggac aaagactcta
     6421 ctattttcat tcttgactaa tctgtctgat gatatctgct ggcgtagtgg cttcttttat
     6481 tccctttcta aaatctgcat tcatcaatcg tctggagagt gtgctgagaa tctctagatg
     6541 catatctgct gatttttcag gaactacgat ggcaaaaata tattcaattg gcatttcatc
     6601 tatcccgcta tagtccgtta atttggctgt atgaaaaaaa cacagtgctg gttttgtcac
     6661 atatttgctt tttgcatggg gaatcccaaa tccatcttct attcctgtgg agatctcctc
     6721 ctctcgtttg aatagtgcct taacgacttt ttgaacattt cttaccttat ttgttttttt
     6781 caacacatcc cctattcgct caaataatga tccttttgaa tctatcgatg gatcgaaaac
     6841 aatcagttct tctgcgatca attgatcaaa cctcataaaa aacctctccc ccacaaaaaa
     6901 tgttaacgtt ttcttggaac tgaggtcagt atagcatcgc tagctgtacc gttcaatatt
     6961 atgaatgaac agaggaaaaa gacccatcat ccgcgtgtat aagtcattaa aatgaacgat
     7021 ctagctcaca aacaatcgca agtctataac aaaacaaaaa gccttccttg aaaggaaagc
     7081 ttagcttcta attttaggta aatggttttt tacttcgaaa taacccctct tcatttcgtt
     7141 aatataaggt gtttttatca agtggataat taaaactata tactagtttt tcattgtttt
     7201 gtgcattatg atcctacgac tttttcagca tcaaacgaag ttttaaattg gcaaaaaaca
     7261 taaaaaaaca gactatccct atcagatagt ctgctaaacg ttgagttatc agcgttttta
     7321 ttattccgca gcgtttgcgt ctttgagacc gtattttttg ttgaaacggt ccacacgtcc
     7381 gtctgcttgt gtgaattttt gacgtccagt gtagaatgga tgtgagtcag aagttacttc
     7441 gacacggatg actgggtatg tattaccgtc ttcccattca actgtttctt gtgaagtttt
     7501 tgttgaacct gacaagaatt tgaaaccagt tgttgaatcc atgaatacta ctgggtggta
     7561 atctggatgg atgttttctt tcatgatttt ttcgctcctt tgccctgctc catttgctga
     7621 accagagttg atttgtcgat ttacaacatt aacatcttaa catgtttcag tcaaggttgc
     7681 aattatcttt tcagatttct ggtcggattt tttttaccaa aggagacatc ttgaaattct
     7741 ttgaaaaatt gctgattgtt ttttgtttta cgcaaaaagc ggatcagttg ttccgtatat
     7801 tctaatgaat ctccagtcat attttttcga agtttccaaa tttcttctaa ttcttcactt
     7861 ggcatcaata attcttcttt tcgggtacct gattttttga tatcgatggc tgggaagatc
     7921 cgacgttcag ataattcacg tgaaagttgt aattctagat ttccagttcc tttgaattct
     7981 tcatagatca cgtcatccat acggcttcct gtatcgacta aagcggtcgc taaaatcgtc
     8041 aaactgccac cttcttcgat gtttcttgct gccccgaaaa atttcttagg tttatacaat
     8101 gcggctgggt caatcccgcc actcaatgta cgtccgcttg gtggaacgac taagttatag
     8161 gcccgagcaa gtctagtaat actatccatc aagataacga catctcgttt atcttcgact
     8221 aaacgcattg cgcgttccaa tactaattca gaaactcgag tatggttttg cggctgcaga
     8281 tcaaatgtag aagataccac gtcgcctttc acgcttcgtt ctagatccgt tacttcttcc
     8341 ggtcgttcat caatcagtag aacgattaat tctacttccg gatagttttc ggtgatacca
     8401 tttgcgattt cttttaatac agaagttttc cctgcttttg gcggtgcgac gatcaatcca
     8461 cgctgaccaa aaccgattgg agaaaagata tcgatcattc gggtagatag tctttgcggt
     8521 gttgtttcca gttgaagctg tctttgcgga taaagaggtg ttaaggctgg aaaatgcgga
     8581 cgttcttttg cttcttccgg atcttttcca ttaacgcttt ccacgtgcat caaaccataa
     8641 taacgttccg attctttagg tggacgggct tttcctgcca ctttgtcccc atttctcaaa
     8701 ctgaatcgtc tgatttgtga ggcagaaata tagatatctt ctgcacttgg tccataattg
     8761 attggccgta aaaaaccgta accatcttga ccaacgatat ccaatattcc ttccatataa
     8821 taaaatcctt gtttctctgc ttgtgcacga atcacagcta gagataattc ctttttattc
     8881 atcttactgt aataaggaat tttaaattct ttggcataac tataaacttc ttttaacgtt
     8941 ttgttctcta gttctgccat tgtcaaataa tcgctcattc agctcccact tcctctatca
     9001 tttcaattga tgcccctaag gctgttagtt tctcgataat atgatcataa ccgcgtaaga
     9061 tataatctac attgtaaatc gtcgttgtcc cttctgccaa tagtccagca atcactaaac
     9121 aagcacctgc ccgaagatca gaagccacga cttctgtgcc gtgaagttta tttggaccat
     9181 tcacgatgat catatttcct tccacggtaa tatcagcgcc cattcgtgct agttccggca
     9241 catggttcac acgtttcgca tagatcgtat cgatgatttc tgaagtgcct gtagtcatca
     9301 atagtaacgc agtaagtggc tgctgcagat ctgtggcaaa tcctggataa ggataggtca
     9361 tgatgtttgc gggttttaat tctttcgagg ggtaaacttc gatttcatct tcgctgattt
     9421 tcatatggac accaatttcc tgcaattttg ctataaagct ttccaaatgt tcaaagatca
     9481 catttttcac tttgatacca tttcctactg cagctgctaa tgctagatag gttcctgctt
     9541 cgatgcggtc aggaatcatg aaatgccgac agccatgtaa tgtttcgact ccttcgatac
     9601 ggatcacatc tgtacccgct cctcgaactt ttgcccccat attattcagt aaagttgcca
     9661 catctatgat ttccggttct cgtgcagcgt tttcaattac tgtctgtcct tttgctttta
     9721 cagcagctaa catcacattg atcgttgctc cgactgaaac cacgtccatg aaaatccgat
     9781 tgccacgcaa tcctttattt tctgtccgta aatacatggc accatgttca tttgtcactt
     9841 cagcacctag agcttcaaat cctttgacgt gtaggtcaat cggtctaggg cctaaataac
     9901 agccacctgg caggcctact accgcttcgc caaatttacc aagcagtgtt cccataaaat
     9961 aataagaagc acgcaaacta ttgatttttc cgtgcggcat cggaatcgat acgacttctc
    10021 gaggatcgat ttctagtatg ttgttggaaa aatggacagt tgcgcccatg atctctaaaa
    10081 tttcaattaa tgaatgaaca tcttgtatgt ccggcacacc ttccaagatc actggggaat
    10141 cagctaagat tgcagctgga atcaatgcga ctgcactatt ttttgcaccg ctgatcgtta
    10201 cttcaccagc taaaggtcgg tttcctttga ttacgatttt tttcatctgt ttccaccttg
    10261 tttattaatc ttttttcatg gatatgcttg tattttatca tagataaaaa aaagaaacga
    10321 gcatggtctg ccgttttaag taaaaaagaa tggagagcga cttcaaaagt cgttctccat
    10381 tcctatatat cgtatctgtt atgtggatgc ttacgctttg ttagctgaac cgaaccagtc
    10441 gatatgagat ttagcatctt caacgattgc tgctttacct ggagctaata atttacgagg
    10501 gtcgaatcct ttaccttctt tatctttacc agcttcgata tatttacgag tagctgcagc
    10561 aaatgataat tggaattcag tgttaacatt tacttttgag atacccattg agatagcttt
    10621 ttcgatttga tcttgaggaa tacctgatcc gccatgtaat actaaaggca tgtctggacc
    10681 tacagcatca gcaattgctt gtaagtggtc aaaagctaat cctttccagt tttctggata
    10741 ttgaccatgg atgttaccga taccgcaagc taagtagtcg atacctgtag caaccatttg
    10801 tttgcattct tcaatatcag ctaattcgcc gttaccgatg attccgtctt cttctccgcc
    10861 gattgaacca acttcacatt caactgagat accttttgca tgtgcttttt caacaacgtc
    10921 tttagctaat ttcaagtttt cttcaaatgg tagatgtgaa ccatcgaaca ttactgaagt
    10981 atagcctaat tcgatacatt ccaaagcagc ttcgtaatca ccatggtcta agtggatagc
    11041 aactggaaca gtgatgttca ttgcttcaac taaatcagtg attaaatctt tacagatttt
    11101 gtaaccaccc atgtatttag ctgcacccat tgatgtttgg ataagtactg gtgcttgttt
    11161 tgcttcagcc gcttcaagga ttgcttgagt ccattccaag ttgtttgtgt tgaatccgcc
    11221 gacagcgtaa ccgcctttac gagcagcttt taaaaattca gctcctgata ctactggcat
    11281 aataaaattc ctcctaagtt ttgtaaaact aaatttgtta agacaattct taaccaacga
    11341 ccctatttta ccaaacattt cggtagtttt ctactaaaat caatgaaaat gaaaattttc
    11401 aagaagtttt ttgcaaaaaa taaaatatcc aatagcgctt taatcgaaaa aagtcttttg
    11461 ctttctatct tttttcctac aaataataac aaatcagaaa aagagaaaag tgacaagaaa
    11521 attgcttatc cccttctctt gctacttttc tctttctatc aagttcaaaa attattttgt
    11581 ttcgtgatct gctaatgatg ctccgacaaa tgctttgatt aatttttgcg ggcgatttgg
    11641 acgagaaatc aattctgggt ggaattggca tcccacaaag aattttttct ctggcagctc
    11701 tacgatttct actaaacggt tatctggtga gacacctgaa aagactagcc cttgttcttc
    11761 aaacaattga cgatatttgt tattgaactc atagcggtga cggtgacgtt cttgcacaac
    11821 atcggctcca tcatatgctt cagccgtctt cgttcctttt ttgattttac atgggtataa
    11881 tccaaggcgt aaagttccac ccaaattctc gatgttttcc tgatcagcca ttaaatcaat
    11941 aatattgttt gctgtatctg gattcgtttc agctgatgac gcatcttcta atccaacgac
    12001 gttacgtgca aattcgacac aagccatctg cataccaaga caaataccta aaaacggaac
    12061 gtcattttcg cgagcataac gaatcgcttc gattttccct tctactccgc gatcaccaaa
    12121 tccaccagga accaagattc catctgcgtc tttcaaacgt tcttctacat tttctctcgt
    12181 taattcttgt gctttgatcc aatcgatctc gatgtctgaa ttataggcga aaccagaatg
    12241 tttcaatgct tcaactactg agatatatgc atctggtaac tcgacatatt taccgacaag
    12301 cgcaatccgt acttttttct tcaagttcaa gacacgttct tctaattctt tccattctgt
    12361 catgtctgct tctggtgtat ctagttttaa atggtcgcaa acaatttgat ccattccttg
    12421 tgcttgtaaa actaatggga tggagtaaag tgtatctaca tctggcgact cgataactgc
    12481 ttctggttcg acatcacaaa attgtgccaa tttgtttttc acgctttgag aaacaggttg
    12541 ctctgtacgt accaccaaaa tattcggttg gatacctaaa ccacgtagct cttttacact
    12601 gtgctgcgtt ggtttagttt tcatttctcc agcagctttc aagtaaggaa tcaatgttgt
    12661 atggatatac ataacattat ctgtaccgac atctgctttc atttgacgaa gtgcttcaag
    12721 gaatggcaaa gattctatat cccctactgt tccccctact tctgtaatga tcacatctga
    12781 atcagtcatt ttagctgctc gcatgatttt ttctttgatc tcatttgtaa tatgaggaat
    12841 aacttgaaca gttgctccca agtattcgcc cttacgttct ttacgcaaaa cttcagaata
    12901 gatttttcct gtcgtcacgt tggagtattt gttcaaattg atgtcaataa aacgttcgta
    12961 gtggcccaga tccaaatctg tttcagcacc atcatctgtt acgaatactt ctccgtgctg
    13021 atatgggctc atcgtccccg gatccacgtt gatgtatgga tcaaattttt ggatagttac
    13081 ttttaagccg cggtttttca ataagcgtcc taaagaagct gcaacaatcc cttttccgat
    13141 tgatgaaacg acaccgccgg tcacaaaaat atattttgtc ataagttcaa aaactccttt
    13201 ttaatttatg tgcaggaaaa gtctcagaca ataaacaaag ctccctattc ttcaataaga
    13261 atagggagcc gaaatttctg taaacttctg accctctaaa agggtgccca aggtgtataa
    13321 tacaagctga ctaagggaaa gtcaagtact caaatgaata ctatccgctt acaatttttt
    13381 ctttttcgat atcctttgag aaattatttc ttaagtcctt tatgagtgca aaaaaaattt
    13441 aacccacata ataagcttga tataaacata gctaacgtta aacctaagca aatcagaaaa
    13501 tcgttactga aaaattttaa gtgaaataat agataaaaaa ataaaacaaa cattatgata
    13561 gataatatcc cacctaacaa agaactactt tttcctactt gcagcaacag tttatatagc
    13621 ttatcgtaga aacaatcttt ttctttcacc cttatttacc tcctttacgt aaaaaacgta
    13681 gtccacagtt ctcggatttt tggcatatct tttctattaa ataatatcaa aaacaaaaca
    13741 attccactaa ttttttcaaa gatgaccttt ttttcaacaa aagtgccttg tagggattca
    13801 ttgaaggtaa cgaatcacat aagtaaaaaa taaggagatg cgacaaaagt cgtgcctcct
    13861 tatttctaaa taaacggtgt tctcctgcca actcctcggt cacaagtcac aatatccatc
    13921 aaacatgaga agctgttttt gaaattattt cttgtgattc ggagttaaat ggtaggctca
    13981 caacctctgg ataaacggta ttcaaaagcc agtttctcgg tattaagcca aatttatgca
    14041 aaaatgtaga aagccatttt cacaaaattt ttcttaatac tcgaaactaa acgtcttttt
    14101 caaaacctct tggtaaacgg tgttcaaaag cttgcatctg cggtaataag ttcagccaat
    14161 tcaaaaacaa gaaaagctgt ttttgcttgg ctattcttat tacttgttgc agggcagctt
    14221 tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt agctcaaaaa
    14281 acggcaaaat atgaggaact atttttccgt tagtttctct aatactcagg tcaaaacgct
    14341 gatttcacag cctctaattc acggtattcc tgttttaata aagttctaga tattgttctc
    14401 tttcccactc ggaaacagtt tgtctgaatg ccgcccattc gagacgtttt gcttcaacaa
    14461 aatttgagaa gatatgttgt cctaatgcgt caatcattac attatcctta cgcaattctt
    14521 tgatagcatt atggatagtt gaaggtaagt cctggatatg tgcagcttct ctttcttcct
    14581 catccattac ataaatgtta cggtcaacag ctgctggcgg tgtgatctga ttacggatac
    14641 cgtctagtcc tgcttgcaac aatactgcca ttgccaagta tgggtttgca gatggatcaa
    14701 ctgaccgcaa ttccaaacgt gtggataaac cgcgagattc tggtacacgt actaatggcg
    14761 aacgattacg gccactccat gcgacataaa caggcgcttc ataacctgga acgagacgtt
    14821 tataagaatt gactgttgga ttgcaaacgg ctgtgtaggc acgagcatgt ttcagcaaac
    14881 cacctaagaa atgataagct gtttcactta gttccaattc accgtcttta tcatagaaaa
    14941 catttccttc atcattgaac aaagacatat tgcaatgcat accagaacca ttgatcccaa
    15001 ataacggttt cggcatgaaa gtcgcgtgaa gtccatgctt gcgtgcaatc gttttgacaa
    15061 cgagcttgaa ggtttggata ttatcacaag catctaccac atctgcatat ttaaagtcaa
    15121 tttcatgctg tcctggcgct acttcatgat gtgaagcttc cacttcaaag cctagacttt
    15181 ctaattccaa cacgatgtca cggcggcaat tttcacctaa atctgttggt gcaaaatcaa
    15241 aataaccacc tttatcattc aaggtcgttg tgatcccacc gtcttcgtct aatttaaata
    15301 agaagaattc tggttcaggt cctaaattga aagaggtaaa accaaggtcg cgcatatctt
    15361 tcaatgctcg tttcaaattc ccgcgaggat cgccggcaaa tggtgtccca tccggattat
    15421 agatatcaca aattaaacga gcaacttttc catgagcact ttcccacggg aagatcatcc
    15481 aagtggagag atctggatac aaatacatat cgctttcttc aattcgaaca aatccttcaa
    15541 tcgaagaacc gtcaaacatc attttatttt ctaatacttt gtcgatctgg ctcactggaa
    15601 cttctacatt tttgatcgta cctaagatgt ctgtgaacat caatcgtaaa aatcgtacgt
    15661 tttcttcctc aatgattcgt ttgatttctt cacctgttat ctgtttcttt accatggtat
    15721 ccctcttcct tttttaattt attcagtcaa agaattgtct ttgcaatcct tgcgactgat
    15781 aatgacctgt tttgcttaac ccaccttgcg ccaaaatctc atcacgaaaa atttgtcgaa
    15841 cgtcatgatc tgtcaacggc ttcttgtttt ttgcttcttg ttttttcgcc tgtgccattt
    15901 cgtatacacg tttgatccca gccatattca atccctctga aagatagtct ttaatctcaa
    15961 gcaacatatc aatatcattc agcgaataca tacgtcggtt tccttcgctt cgttccggat
    16021 gaactaaatc ttgttcttca taatagcgaa tttgccgagc agacaaatca gtcagcttca
    16081 taactgtacc aattggaaat acggacatag atcggcgcaa ttccttttct cccatcagac
    16141 gtcccctttc gttttataag ttggctaaag tgtaccaatt aaaaaaaata ttgtcaactg
    16201 catatgttag gttttctaac atgagatctc tattttttat aaaataacaa aaaaataaga
    16261 acacaaaaaa ggtatctagt tgattttcgt accagatacc ttttattttt tattatactg
    16321 tactatcctt tattagtgtt tttatt
//
LOCUS       DACDXE010000069        15687 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.069, whole genome shotgun sequence.
ACCESSION   DACDXE010000069 DACDXE010000000
VERSION     DACDXE010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15687
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.069"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            68..751
                     /locus_tag="IWM23_002226"
     CDS             68..751
                     /locus_tag="IWM23_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAP9884835.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            753..1589
                     /locus_tag="IWM23_002227"
     CDS             753..1589
                     /locus_tag="IWM23_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9884836.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            1600..3354
                     /locus_tag="IWM23_002228"
     CDS             1600..3354
                     /locus_tag="IWM23_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884837.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(3630..3971)
                     /locus_tag="IWM23_002229"
     CDS             complement(3630..3971)
                     /locus_tag="IWM23_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAP9884838.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(4039..6210)
                     /locus_tag="IWM23_002230"
     CDS             complement(4039..6210)
                     /locus_tag="IWM23_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAP9884839.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            6421..7278
                     /locus_tag="IWM23_002231"
     CDS             6421..7278
                     /locus_tag="IWM23_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAP9884840.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(7349..8206)
                     /locus_tag="IWM23_002232"
     CDS             complement(7349..8206)
                     /locus_tag="IWM23_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9884841.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(8191..10893)
                     /locus_tag="IWM23_002233"
     CDS             complement(8191..10893)
                     /locus_tag="IWM23_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9884842.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(10906..12195)
                     /locus_tag="IWM23_002234"
     CDS             complement(10906..12195)
                     /locus_tag="IWM23_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAP9884843.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            12345..13391
                     /locus_tag="IWM23_002235"
     CDS             12345..13391
                     /locus_tag="IWM23_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9884844.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            13393..15546
                     /locus_tag="IWM23_002236"
     CDS             13393..15546
                     /locus_tag="IWM23_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAP9884845.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT         4454 a         3282 c         2887 g         5064 t
ORIGIN      
        1 ttgctccgtc ttcttctctt gctgttctgc ttcattgcct ataaaccttt atgaaagaag
       61 agataacatg cctactatcc atattgccgg tgattctact gcggcagaaa aagatgaaaa
      121 caagcgtccc gagacaggtt ggggagaaaa aatctctgct tatttttcac ccgagatcca
      181 gatcaataac caagcaaaga atggacgcag caccaaatca tttatccagg aaggaaggtt
      241 agcccgtcta gcttcacagt ttcagccgaa tgatttctta tttgtacaat ttggacataa
      301 cgaccagaaa atccaagaag aaagaggtac gcttcccttc ggtagctatt taaaaaatct
      361 gcagatattt tttgaatgcg caaaaaaagc gaacgtccaa atggtcttac tgacatctgt
      421 gacgcgacga gattacttag aaaacggaac attgaatcca gatatacttg gagactatcc
      481 aaaagcgatg cgtgcatttg ctgagaaaca ccatattcct cttttagatg tgttctcaag
      541 atcacaagaa ttgttccaga ccttctcaaa agaagagaca aaaaaattct acctgcatct
      601 tatgcctgat acttgtaaaa attacccaga aggactaaaa gacaataccc actttagtcc
      661 agaaggcgct gataaagttg cccgaataat tgttgaattg ataaaagaat cccgtctgcc
      721 tcttgctaat tatttacaaa aaggagtttg agatgtttcc attaaattta ggcatccgtg
      781 cccacgattt agatgttcga aatcgtgaag aaatcgttca aaaagtatca cagcttcact
      841 tgtcccatat ccaatttgca ccacaaaaag catttcctga acaacttaaa ttaactgaca
      901 tgacacttgg gagtgctgcc tattttggtg aatacttcca aaaaaacaat atcgaacttt
      961 ccattttagg atgctatatc aacctatcca gcaaaaatca agaagttcgt gcacaagcat
     1021 tggcaacttt caaacagcaa attgctctgt gctctgccta tcaagccaag atgattgcta
     1081 cggaaaccgg tagtgtctct gtcggttaca caaaagaaaa ttttactgaa gaagcttatc
     1141 agatcgccag aaattctatc attgaattag tcgctcatgc agaaaatttt ggcgtaactg
     1201 ttgctgttga agcagggatc aatcatccaa tctataacta tcagttagct aaacgtttaa
     1261 tcgatgaagt agcttcacca aatttaaaaa tcattttaga ttgtgcgaat ttgatgcata
     1321 aagaaaatca tgctgatcag gaaaaagtga tccgaggtgc cttggacaat ttacatggtt
     1381 atattacagc gcttcatctc aaagactata tcatggaaaa tggaaagatc cgcatggtac
     1441 ctgttggcaa gggctgttta gatttccgcc cggtactgca ttatatcaaa tatgagcgtc
     1501 ctctaatgta cgccactctt gaagctactc ctgagattta tgttcctgaa gcaatagaac
     1561 acttgcagtt tatttaccaa gattgttaag gagaagatta tggaggaacg aatacagaaa
     1621 agttatcaaa aatatcatgc aaatgctttt cagacattgc tgcatcttta tcagggaaat
     1681 tattggcgtt ttgccatgtc aggactcttt tttattatca aacatcttcc atcttggtta
     1741 atgccgatcg ctattgccaa tgtaatcaat gcagcttcat cgaaagattc ttctcagttg
     1801 caggccattt atttcaacgc ttttttgatg gcttgtctga ttggtcaaaa tatattgacg
     1861 aattattttc atgtaaaata ccatagcact tcgatccgtc aagtagaagc tggactaaga
     1921 gcgaccctta tcaagaaaat ccaagaacta tctattcctt atcaaaaaga attgcaatct
     1981 ggacgaatcc agtcaaagat catccgtgat gtagaagcta tccaaacgtt gtcctctcaa
     2041 gtatttgtca gcggattaaa tatcgctgtc aatttattcg tagctttagg tatcacgctt
     2101 tataaaagtc ctgttgtttt catgtttttc ttattgactg ttcctctcgc cgcattcgtt
     2161 actctctttt tccgtaaaag gatcagacag tccaactcag acttcaggca agaaatggaa
     2221 gagacttctg caaaagtgat cgaagtgatc gaattagcac cgattgctcg agctcatggg
     2281 ttagaaaaac acgaaacaac tcgcttaaat aagcatttta ctcaaatcta tgaaaaagga
     2341 ttccacttag atatcctgca atctgttttc ggatcagtca gctgggctag ttttcagata
     2401 tttcagctga tttgtttgat tttcactggg atattagctt tacaagggcg catccaaccc
     2461 ggcgatgtcg tgatgtatca gacgtatttc acgacgatcg tcaattccgt ttctggaatg
     2521 atcacgctga ttcctacgat ttcaaaaggc atggaatcta tcacttctat tggcgaactg
     2581 ttgattgctg gcgatgtaga aacttatcac ggtaagaaaa aaatccaaca tttggatgga
     2641 aattttcgtt ttgaggatgt ttcttttctt tatccggact cactcaaacc gattatcgat
     2701 catttttctc tagaggtgaa agctggagaa acaattgctt ttgttgggcc atctggctct
     2761 ggaaaatcta cactccttaa cttattgatt ggatttatcc agccgacttc aggaaaaatt
     2821 tttctagatg atcagccttt ggatgaactg gatatgcgaa gtgtgcgaaa ttatttagcc
     2881 gttgttcctc aaaccacttt gttgttctcc gcttcgatca aagagaacat cacttatggc
     2941 ttgaaaaacg tatcaaaaga acgtttagat gaagtcatcg aagctgcgca actttcttca
     3001 ctgattgacc agcttcctga tggtctggat accttagtag gcgaacatgg caacaagctg
     3061 tctggtggcc aaaagcaatg tatctccatt gcacgagcgt tgatacgtca gccaaaaatc
     3121 attttgctag atgaagcaac ctcagcttta gataaccagt cagagaaaaa aatccagcaa
     3181 gccctgaatt acctgacaga aacacccact acttttattg tggctcatcg gctttcaacg
     3241 atcaaagagg cagataaaat cgttgtgatc gatgaaggaa aaatcgttga aataggtact
     3301 tacgaaaaac tgttagctca aaaaggctat ttctaccgct tgaaaaatga atgacaaaaa
     3361 ataggagcta tgacaagact tttgtcgtag ctcctatttc tgaataaacg gtgttctcct
     3421 gccaactcct cggtcacaag tcacaatagt catcaaacat gagaagctgt tttttgaaat
     3481 tgttccttgt aactcagagt taaacggtag gctcacgacc tcttggtaaa tggtgttcaa
     3541 gagacagcct atcggcaata agaagcaatt ttgtcatcgc tcaaggccga acgtctcttt
     3601 cacaccctct gttgtttcat aaaatagttt tattcgctgt aaaggtcgcg aactggtgtc
     3661 atgatgatac ggttcaagtc attgatgatt gtactgaagc cttgctcttt ttgcatcaag
     3721 tcattgatta cttcttctga ttggattttc acagccattt cttgtgcttt cattgcatct
     3781 tcgtcgctga actcttgtcc actcatttgt ttttcttgta attcgatttg catcgcttgg
     3841 aagtctttga acagtttgtg tgcttcttcg tttgcttgta ctttctcgta cgcttctttc
     3901 aacgctttga attctggtaa ttcacggatc tcacgttcga tttgattcgc gctatcgtag
     3961 atgtttgaca tgttatgccc tcttttcttt tagtagttac tctctattgt aacatggggt
     4021 tattcgtttt caaaggggct actctccttg atatttccgc cagaggtctt tagccgcttc
     4081 accaattccc tgaacgcctt ctttcaccat atcgccgaag tttttcagcc cttcttttgc
     4141 ttgttcacca tattgattca agttatcttg ccaatttcca gactctccat tagaatttcc
     4201 tgtgtttcca acttgatctg cagctaccac ttgtcctcct gtggcatagg catctgctac
     4261 actaaatcct gtttttgaag tatacggaag gatgccttct gccgctgatt tgaagacttt
     4321 cccgacaaca tttccacttg tcccttccaa atagtgcagc ttgctggatt cttcgtaacc
     4381 catccatgtc gaaatcacta cgtctggcgt gtaaccgatc atccattggt catttgcttt
     4441 tgtggcatcg aaattagttt ctgtggttcc tgttttccct gctatcgtat aacctgcagg
     4501 attcgcagca gccgctgttc catttgaaaa tgtaccaaga agcatgctgg tcatttcatc
     4561 ggctgtttcc tttgatatga cctgttttgg ttttttgttt gtgttatcga cgatcaccgc
     4621 tccagtcgaa tcaacgattt tcgtgataag atgcggcgta tacatctgac catcattggc
     4681 aaatgcactg taagcacccg ccatgatcaa tggagatact cctttttcca gtccaccaag
     4741 ggctaagcca taatacttat cagattttgc taaaggcagg ccgaattctt ctgctttatc
     4801 atatcctttt tgtaaaccga tctcatgcaa aagccataca gcaggcaaat tcaaactttg
     4861 tgcaacagct tgatacatcg gtacttctcc ttggtatgtc ccgtcaaaat tatgcgcatc
     4921 gtaataactt tgcggttcgt ctttcaagat actggaaggc ttgtatccag cttccagtgc
     4981 tggtgtatat acgcttaatg gtttgatcgt tgatcctggt gaacgacgca tctgtgtagc
     5041 aaagctgaat cctctaaaga catgatctcc tcttcgtcct actagtgctt gtacaccacc
     5101 tgtattcgga tctaaagcta ctgaagcaga ttggaccatg gcaccatctg aagcatttgc
     5161 tgggaataat gcactgtttt gataagtagc ttctaattgc ttttgataat cctgatctaa
     5221 agctgtatag attttatagc ctttattcat gacatcttct tcatcaagac catactcgtt
     5281 aactgcttca tcgatcactg catcaaaata ataaggataa cgataaccag aatctgaatt
     5341 atcgtatgtg tcattaagat acgcgctgat atctgtctgg ctttctgctt gttcttgttc
     5401 ttttgtgatt tttccgtttt cttccattac acttagtact gtgtttttcc gatcattcgc
     5461 attttttggg tgatcgatcg gattataaat accaggacct ttcagcattc ctgccaaagt
     5521 agcagcctca gacagcgtaa cattcgttgc atctactcca aaatactttc tggaagcgtc
     5581 ttggacaccc cataccccgt tgccaaaata ggcattgtta agatacatgg tcaagatttc
     5641 ttctttgctg tacttctttt cgatctcgat tgccaaaaat aattccttcg cttttcgatc
     5701 aaacgtttgg tctaatgtca gatacgcatt tttggcaagc tgctgagtga tcgtacttcc
     5761 tccgccccct gatgttccgc cagacaagac cattcggact gctgcacggg caatcccttt
     5821 gatatcaaac ccgtgatgct cgtaaaaatt cctatcttct gtcgagatta ctgcatcttg
     5881 gatataggga gaaatcgcgt ttaattctac atatgttcct ttttgggcgt acagtgtgcc
     5941 agcttcttcg tttttcttat cataaatgac tgtcgattgg cttaatcctg attttaacgt
     6001 ttcgacattt gcttgttttg ctaaaataaa gaggtagata cttgtcacta agacacagat
     6061 caaaccaatc agcaaaatca atttgttcac atgatatttt ttccagatgc gctttcgcca
     6121 tcgatggaat tgtccgagat atggtctgat ccaacgccaa aaactgatca aagcagcttt
     6181 tatcttttct aaaatgcttt gaaaatccat ttgcttcttc tcctgtctat ttgattcaat
     6241 cgtcttattt taccatagtg aaagagtgtt ttcttcctgc ttaagttgta gttcaatatg
     6301 tttttttacg ttagtgcaag tagagtaaca taagaatgaa cagaaaaatg taagtttttt
     6361 cttaaatatg ttttaaaaat ggtacaatta gtctgcaatt gcactgaaag gagtacactt
     6421 gtgaaaatca cgatcacttt acctatgaac catcctgaaa caacaattcg cgacttgctt
     6481 gaaaaggaat ggttagttcc tagaaaggtt cgacactttt tgcgtatacg taaaaacgtt
     6541 tggatcaatc aacaacctgc tttgttccat catgaagtaa agggcaatga ccagatcaca
     6601 ttgttattcg aagaaacaga ttataattat caagaagttc aattaggaga tgcgaagcac
     6661 atccgtgttt tatatgaaga tgagcatttg attatagtgg ataagcctgc aggaatgaag
     6721 actcatccaa atgaaccgac agaaaacagt accttactca atcatttagc tgcttatctc
     6781 gacactaaac agcaacgacc ttacgttgtc caccgcttgg acaaagaaac aagcggtgcc
     6841 gttttatttg caaaaaatcc atttgtcttg ccgatacttg gaagaatgtt ggaacaaaaa
     6901 atgatctatc ggagatatca agccatcgta tggggagaaa ttgccaaaga tatgaccatc
     6961 accaataaaa ttggacgaga tcgccatgat cggagaaaaa gggtcgtaga tgataaaaat
     7021 ggtcagagcg ccttgacaca tgtcactgtc aacaaagtac tttctgaaca aacacaagtc
     7081 tattgtgttt tggaaaccgg acgtacacac cagatcagag ttcatttagc tcataaggga
     7141 catccgatcg ttggagaccc tctctaccaa aatagaacag ctgaacgact gatgctccat
     7201 gcattagaac tgcacatgat ccatccattt actcaagaaa aaatagtcgg taaagcttta
     7261 cctggattat gggaatagaa ataaaaaaga acagagtctt atcagcagat aatgataaga
     7321 ctctgttctt tttgtgtaat ttgtctgctt acttttgatg ttcgtgttct gctaacagca
     7381 aactaccgat aatcccactt tcattcggca gtccccaacg aacgatatat tcacttactt
     7441 gaggtgtttc catataacca cccatcaatt caacaaattg ctgacggatt ttttgaagga
     7501 gatgatcctg attcattacc ccaccgccta aaataatctt ttcaggtgct aacgtcaacg
     7561 tatagttgat cagtgcttga gcaatataat aagcctgaat agcccaaaca ggatgatctt
     7621 gcggcagatc ttgccctttg atccctgtgc gtgcctcaat agacggtcct gctgctaatc
     7681 cttctaaaca atctttatga taaggacaag taccttcata atggtcatct ggatggcgtt
     7741 tcacccaaat atgtcccatt tcaggatgtg cgatccctga aaaaatttct tcattgatca
     7801 cgacacctgc accgattcct gttcccacgg taaggtaaat acaactattt tttccttgtg
     7861 ctgctccttg cttcaattcc ccatatgcag cagcattgac atccgttgtc caagcagcag
     7921 gaatattgta gcgttccttg attgtgccaa gaaaatcgaa ttgtgcccac ccttttttgg
     7981 gagttgccaa aacgtatccg taattatctt ttgttttgtc gataccaatc gggccgaatg
     8041 agccaatccc gattcccttc aattcataac gatcaaaaaa ttcaaatacc tgatccaatg
     8101 tttcttctgg tgttgtggta gggatactta atttttctaa aatttctcca ccatctgaaa
     8161 ccgcacacac aaattttgtt ccgcctgctt ctattccgcc atacattcgt tttcctcctc
     8221 gaatccgatt gtccgaatct cataaggttg gattattggg acaaccgctt gcttcgttgg
     8281 ttcttcaagc agattcaata tgacagtttt tccattgtct ttagttattt ccaatttttc
     8341 taaatgacta gacatattga aacctcttac tactacttta tcactaaact tgctgcgttt
     8401 caaagctgta attgcaaacg tttcactttt tatcgttaaa tattgttgtt ttgaaatcaa
     8461 tgttccttca tgatgtttga tttgttgagt ggaaaaagga atctgtgctg cgtatgcatg
     8521 caaataagta gagaattttt cttctggtcc atgtagttca atgctgtaat tgaaccgatg
     8581 ctcacctaga cactgtgctt ctggtgttgg aaaatatccc caatcgccta gttctcccac
     8641 actgcgaagt agcgtcactg cgatttgtcc gtcttctgtt acttcatatt cattcaaacc
     8701 aaagtttccg actgttacac cgtactcttc atcatgaata ttgacaaatg cttgctgatg
     8761 ttgcggattc gtggggtttt cccaactttt agacacatgg tttgggcgct tcaccacttc
     8821 aaaaatacta tctgcttcgt gatgttccac atgtaacttt gtaggaaaca agacacgtaa
     8881 gcgatgatct ttcatctgat tgtcgagagt cgtttcaaag tcgatttttt tactatcttt
     8941 tctgattgtc atgattgttt ttatttgaag gattcgtttt tctttggatc gttctgcttt
     9001 tcgataacga aattccacaa ccatctgctg ttctttttct aaacgctcgt ctgcagaaac
     9061 agggatttcc atttcctgta gaatacttat ttttgctatt tccggtgtat ctactaatac
     9121 ttttacttca gagttttctt tgttactgga taggattggc tgatcacaga aaggttgttt
     9181 aaagatatac tcatttccta tatcgccgat atcttcaaaa atatgcagat tattcaactt
     9241 tcgtttgttt ttgcgatctt ccattgtaat ggtaccattt ttttcaatcg tcaaatgtag
     9301 agggccgttt tcgatgattc gtccactctg atgaatcatt gtttcctttt cttctgcttt
     9361 cgcttcccct tctacaagcg cgaatgtttc ccaagaaaat gctggcatgt tttctaatgg
     9421 taaagtcact ttgatcattc ttgccatata tggcacacgg aaacggtctt tcggcaaatc
     9481 ataaccaaat tggacagttt cttcagataa aatagctggt atagtggccc cactttttgt
     9541 ttctacatga tacttacgtt taggcaaatt ttctaactct tgatatagtt gttcaggtat
     9601 tccttcagcg aatctttttc tttctaattc gatggttatt tctgcttcac cagttttatc
     9661 cattcctgca gtatttacaa tgacaaatgg aaagctgtct tttgggaatc cggtcgtatc
     9721 gattgctgcg gtcagctgtt ctaaggaatc ttgtgcaaga tactttccaa cttcatctgc
     9781 tttttcaaaa cgaggtatca tttcccgatg gacagaatcg actgaacaac cgcaaatact
     9841 gtcgtgagga tgattttgca ttaatgtctt ccaagcataa tctaattgat catggggata
     9901 gtttccacta acttcataag ccatcgtcgc aagtggttct gtaatatttt ctagctgtcg
     9961 ctggaccttg gtattccatt gttttaagta aacccgagca gatgctgtat tggctaaagt
    10021 ataccagcca tccgtttctt gacttgtcaa ttctccttct actgagccta agtcttctgg
    10081 cacatctttt tgaactgctt ccagatagtc agtaaaatta gaatgaataa actcgtaatc
    10141 tggaaacagt tcatttgcga gatggattgc ttttgatata tctttttgta ccggttgatg
    10201 atcaacacca ttcatcatca ataagtggtt cgtagaggca tattgttctg catccgctaa
    10261 tttttgcttc caaaaggcaa gagctgcttc tttttctact ggaatctcat tcccgttgct
    10321 ataccaattg gcaaatagca gaccaaagat tgctgtttga tcagggcctt tccaccacat
    10381 ctctgagtat tgggaagaat agttttctgc ttccaaaact tgattatcaa agccaattgg
    10441 ttttacacca cggccaaacg ctgctgctga aataccagct tgtttcatta attgaggcgt
    10501 ctgtcccata tttccaaatg tatcgggaaa atagcctaac atgacagggg ttccccattt
    10561 acggctttct tccatgccaa tcagcatatt tctcacgttc gattccgagc tgataagaaa
    10621 atcatcttgc aagatataaa atggtccaat tctcaatttt ccttcattga ttgcttgttg
    10681 gattgcctgt cttttttctg gccttacttg aagatagtca tctaaaataa tcgtttgccc
    10741 atccaaatga aaactgttga aggacggatc aacttcaaat agctctagta gatcgtctat
    10801 caattcaatc agtcgcatgt gatgctgttc gtaagccatg taccattcac gatcccagtg
    10861 gctatgagaa atgatatata ctttcttttt catttacgat acctcctact tttctactct
    10921 aatatcaaag taatccatga ctaattcgca aaacatcata tttgcccaag aaaaccattc
    10981 tctcgtataa ttttcaggat tgttcacatc gaatccttcg tgcataaggt gtgttcctgc
    11041 gtcagttgct acaagtaaat tcaatatccg ctctttttca gatttgtctg acgttgtcat
    11101 tccttccata gctaatgcaa tcggccaaac gtatttttct ggcgtatggg aggatccaat
    11161 tccttttgcg tactttcctt catagaaata tggattttct ttactcaaca atgttcttct
    11221 tgtttgcaga taatcttcct cttgttcaga acaatatccc aagtaaggtg cggcgatcag
    11281 attgggcaca ttactgtcat ccatgactgt ggcgtttccc attccatcta cttcatatgc
    11341 ataaatcgtt tgcccttttt gattaaccgt tcgtccgtga ttctgaatcc cctcttggat
    11401 ctctttcttc aatttctgtg tttgctgtac aatttcttta tcatcgagta tcgtttgaaa
    11461 aatttcttcc acataaccta acacgaccac cgcaaacata ttggatggta ccagatatcc
    11521 ataacggcaa gcatcatcgc taggacggaa accagaccat gtcattcctg taaaagctgt
    11581 ttttgcacct cgcccgtcat tgatcagcgt atcttctttt cttgtcgtgt ctcgtacaaa
    11641 tgtataagga gagtgttcat gatcttgctc tgttttgaac acgtccaaga tttttttgat
    11701 accttcaata aaagattgat tgaattgatc agtttttcct gtatttttgt aaagaagata
    11761 ggccaactgt attgggtagc ataaagaatc aatttcatat tttctttccc agatccaatc
    11821 gttcattttt gtatgatctg tttggtggcc agcaccgtta gcttcttcgt tgaaggcatt
    11881 cgcatatgga tcgatattga tataaaaaaa ttgctttttg actaacccgc tgatcatgtc
    11941 agctaattcc tcatcttctt ttgcaatcac taaataagga cgaacttgtg cagtagagtc
    12001 tcttaaccac atcgctggaa tgtctcccgt caataaaaag gtggttccgt cattttgctt
    12061 tttgactgtt gtcaacagag tattggcaaa tgctgctgta aaattctttg cccagtccgc
    12121 atggatatcc ccacattttt gtgtgatatc ttccataaat ttctgcaccg attttggtat
    12181 atctttataa accactaata aaaccttctt tctatttgat atatctttaa agattatact
    12241 tcatacagcc aattatgtct acgctttcaa aaaatatctt tttatttact ttttcatcca
    12301 ttatggtatt atttgaatat gatatatcat taagaggtgt ttatatgact caaccattgt
    12361 accagacaat atttaatgat ttgcaaacgg ctattttaac tggtgaatta cctgtagaca
    12421 gccaactacc aactgaaaaa gaactttcac gtacctataa agtaagcaga atcacttcca
    12481 aacgtgcatt gactgaatta gagcaattag gacttattta tcgcgttcgc gggaaaggca
    12541 gtttcgttaa atccgcttct ccggaacaga ttcttgcttc tcctacaaaa acacgacgga
    12601 ttctgtttct tcttccatat gtcgctgatt tgtctgttgg tgattttagc aagggattga
    12661 accctgtgat gcaagataaa cagattgatg tcatgatgac gacgcttgac tttctacaaa
    12721 ataaaacagc aaaagagcta atcaatgaat ttgacggatt gatttattat gctcttgata
    12781 cagaacaaca tcttgactta ctttttgagc tttcattgaa agaattccct gtcgtcatat
    12841 tagataaaaa aatctatgag ttaccttttc ctaccgttct ttccgataat ttccaaggcg
    12901 gtgcgcttgc tgcctcttat ttgatcgaac agggacattc aaaaatcgct tatttgtttg
    12961 gtacgcagac tcatccacaa tccgttcgac agcgttacct tggttacctt gaagcattgg
    13021 acaaagctgg tttgactttc cacacttcac tggatgacaa aagcgcgaca ttagatgaat
    13081 tgctttctta tatcactaag tatgaagtaa ctgcttttgt ttgtgaaaac gatgttgtcg
    13141 ctatccagac catgaatctc ttgagacagc atggttatca tatccctgaa gatttttcaa
    13201 ttatcggatt tgatgatatc caagcagcag ctcttgttga cccgccactg acaaccatcg
    13261 cacaagattt tgaacagtta ggtaagcttg cgggagatgc tttggtcact tggttagaga
    13321 caaaagatcg accatccgat agtaaatttc cagtttcatt catcaaaaga caatcaacaa
    13381 aggagaaata aaatgaatat cggtcaaata gatactagac atgcaacagc gaatcagcca
    13441 aatttctcta atgggaactg ccagccttat actggcgttc cttttgggat gaattatttt
    13501 gcaccgcaaa ctacggatca aaacggcagc tggtggtttc atcctgatga ccgcgttttt
    13561 caaggctacc gtcttaccca tcaaccaagt ccttggatgg gtgatttcag tcatatgctg
    13621 tttacgccga tcaatggaaa actacaggag aacacccttt tccatgctca aagttcttac
    13681 cgcccagaag aaagtatatt ttgcccgact catctgtcga tctgtcaatt acgttatggc
    13741 atctgttcta cattgatacc tagcatgtat ggcggaatct tatctattga ttacagcaaa
    13801 aatgacagcg gtttactttt atcctttccg ggacgccatc agctctttgt tatagatcct
    13861 tacacagtgg aaggaaaaat cacacatttt gctggttcag aagatcctga tttttctttt
    13921 tattttatat tgagatttga atatccttta acaattgatg agttagctgt ttccaacaaa
    13981 aatagcgact cgatcccgtt gtattttgaa cagacaaaaa aacaaactat ccgatttggg
    14041 acatctttta tcagctcgga acaagcagct tataacctgg aactagaaca aaaccaaagc
    14101 aaagagactt accttgctca cagtcaagaa caatggcagt cctattttga tcgtatacag
    14161 ctcacacacc atgatcaaca acaattgcgg acgttttatc ataatttata tcgggtgttt
    14221 ttatttccac aaacatttta tgaaattgat cctagtgggc aaaaaatcca ctatgatacg
    14281 actagtcgat ctataaaacc tggtgttctc tatacaaata atgggttttg ggatacgtat
    14341 aaaacggttt atcctcttta ttctttgatt gctgtagaaa aatacgaaga aatgctggaa
    14401 ggttttttga acagctatag agaaagtggc tatttgccaa aatggctgtc tcctgatgaa
    14461 cgtggactta tgcctgggac attgatcgat gcggtcattg cagatgctgc tgtaaaagat
    14521 atccgacgag acttgatgcc tgaatttcta acggcaatgg aaaaaggtgc tacggttcaa
    14581 agcaaacatc caaattatgg acgccaagga acagaagatt acttgacata tggctatgtt
    14641 cctaatcact accacgaatc agtcaatcac acattagact atgcttatag cgatttttgt
    14701 attagccaag ttgccaaggt actagaagaa gaggaaatga cgaagaaata tcagcagcaa
    14761 tcattgaact atcaaaatat ctttgactat gagtcaggat ttatgcgttc aaaagataaa
    14821 gatggcaagt tcagagaacc attttcagct aatcgttggg gacaagatta tgcagaagga
    14881 agtgcttggc aaagcagctt tgctgtttat cacgattttt caggactgat cggaaaatat
    14941 ggtggcagtg agagatataa agaaaaactc attgagctgt gcaatcagcc cccaacgttt
    15001 gacccaggag gatatggttt tgagatccac gaaatgagtg agatggccgc gatagaattt
    15061 ggacaattag ctatttcaaa ccagccaagt tttcactatc cttacttgtt cagttatata
    15121 ggacaacctg aaatggcaca gccactgctt aaacagttga tgactcagac cttcgacgat
    15181 tctccaatgg gttaccctgg agatgaggac aatggaagta tggccgcttg gtatatcttc
    15241 aatagtctgg gattttatcc tgtcacgcct ggaaccggtg aatatgttat cggtatgcca
    15301 ttaattgaca aggctgtttt gcttttgtca aatggaaagg aattgacgat tgaagcaacg
    15361 cctaatcggc cgcagcaaca atttatccat aaaatcaaac ggatggacag agaatatacg
    15421 aaattatatt ttacccatca agatttgctg aaaggcggca cgatctctta ccaattaggg
    15481 atcatccctc accctaagaa atatcaagca aaagatttgc ctttttcctt atcaaactat
    15541 aattaacaag aggttgtgaa aaagctgtcc tgcatcaagt aataagaaca gccaagcaaa
    15601 aatagatcct catatttttc gtttggttgg tcttattacc gcagatgcaa gcttttgaac
    15661 accgtttatt gagaggttgt gaaatca
//
LOCUS       DACDXE010000070        15230 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.070, whole genome shotgun sequence.
ACCESSION   DACDXE010000070 DACDXE010000000
VERSION     DACDXE010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.070"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            278..787
                     /locus_tag="IWM23_002237"
     CDS             278..787
                     /locus_tag="IWM23_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAP9884846.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="IWM23_002238"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="IWM23_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9884847.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="IWM23_002239"
     CDS             2322..3302
                     /locus_tag="IWM23_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884848.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="IWM23_002240"
     CDS             3424..4842
                     /locus_tag="IWM23_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9884849.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="IWM23_002241"
     CDS             4913..6382
                     /locus_tag="IWM23_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9884850.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="IWM23_002242"
     CDS             6392..6859
                     /locus_tag="IWM23_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9884851.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="IWM23_002243"
     CDS             6895..7767
                     /locus_tag="IWM23_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9884852.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="IWM23_002244"
     CDS             7848..10382
                     /locus_tag="IWM23_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9884853.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="IWM23_002245"
     CDS             10538..11311
                     /locus_tag="IWM23_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9884854.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="IWM23_002246"
     CDS             11304..12095
                     /locus_tag="IWM23_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9884855.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="IWM23_002247"
     CDS             12105..12596
                     /locus_tag="IWM23_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9884856.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="IWM23_002248"
     CDS             12614..13024
                     /locus_tag="IWM23_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9884857.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="IWM23_002249"
     CDS             13028..14428
                     /locus_tag="IWM23_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HAP9884858.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="IWM23_002250"
     CDS             14451..14825
                     /locus_tag="IWM23_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAP9884859.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DACDXE010000071        14913 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.071, whole genome shotgun sequence.
ACCESSION   DACDXE010000071 DACDXE010000000
VERSION     DACDXE010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14913
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.071"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(389..1117)
                     /locus_tag="IWM23_002251"
     CDS             complement(389..1117)
                     /locus_tag="IWM23_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884860.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1095..2669)
                     /locus_tag="IWM23_002252"
     CDS             complement(1095..2669)
                     /locus_tag="IWM23_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAP9884861.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2898..3836
                     /locus_tag="IWM23_002253"
     CDS             2898..3836
                     /locus_tag="IWM23_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884862.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3848..4759
                     /locus_tag="IWM23_002254"
     CDS             3848..4759
                     /locus_tag="IWM23_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884863.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4783..6255
                     /locus_tag="IWM23_002255"
     CDS             4783..6255
                     /locus_tag="IWM23_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9884864.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6306..6917
                     /locus_tag="IWM23_002256"
     CDS             6306..6917
                     /locus_tag="IWM23_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAP9884865.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6947..8860
                     /locus_tag="IWM23_002257"
     CDS             6947..8860
                     /locus_tag="IWM23_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HAP9884866.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8988..11969
                     /locus_tag="IWM23_002258"
     CDS             8988..11969
                     /locus_tag="IWM23_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HAP9884867.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11966..13627
                     /locus_tag="IWM23_002259"
     CDS             11966..13627
                     /locus_tag="IWM23_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HAP9884868.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13667..14599
                     /locus_tag="IWM23_002260"
     CDS             13667..14599
                     /locus_tag="IWM23_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HAP9884869.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4850 a         2355 c         2996 g         4712 t
ORIGIN      
        1 aactaaaaaa atatgcggcg cacagaacgc gccgcatatc ggactaagtt ttattcaaat
       61 ttgtcttcac caacacgatt tgacgaagag cctatttaaa taactacatc catttattct
      121 atcttctaaa atagcctttt tcatttcagt taaatagttc agcgccattt ttgcactcga
      181 cttgcatagt agttttttcc atattcctca acctcagcgg gggttgaaga cataaggaaa
      241 atcctatgcg ttatgctcaa ctggatctga ttaaatattc taatgtattt ttctctacca
      301 tcattaaatg gttaatagcc aaaaatcacc acttttcaat ggctccttgt tgtataagta
      361 taactagaag atgaacacgt ctgagaaatt atagaagctt tttacggtaa ttactggggc
      421 tgtgtccagt agtttttttg aagagaacag aaaaatagct cacgtgatct attcctactt
      481 tttgactaat ttcatcaata gataagaatg tgtctttcag tagagcactt gctcttttga
      541 ttcgaaagtc tagcaagtaa tcatgaaagg aataacctgt ttcttttttg aaggcccgtc
      601 ctaaataagc gggatggtaa tggaattgtg tagctacatc tttgagcgta agcttttcac
      661 tataattgtt ttgaacgtaa tcaatcactt gttgaattga ctcattaaaa gaatcatcag
      721 ttagagagtt ttgattagct gctgatttac gcattttttc ttcttgtact aacttaacta
      781 aataaggaaa atcaatgggt ttcgttatgt aacttttaat agataaatca atcccctttt
      841 ttgcatactg aaaattttgg tgggcactaa tgacaatact agtaaagtag cgattggatg
      901 tttgttgcaa agattcgatt aattcgagtc cagaaatttt ccccatcgta atatctgtga
      961 taagtaaatc aaagcgctgg ttttgcaggt ttttaaatgc ttcgtctccg tctgtgtagc
     1021 agtgatcgat aatgatgtcg gggactttct tcaaaagata atgccttatg ccttctacaa
     1081 ttgtacaatc atcgtcaaca attgcaattc tcaacatgca tattcccccc tttttgtatt
     1141 ttgatagtaa tcgttgttcc gacgtgtagt tggctttcta taacaaaacg tgtatctcca
     1201 ggatagagat gctttatacg tcgaaaagaa tttgagatgc cgatattctc actataagga
     1261 atatcatcat taagtttttg ttgaatatgc tccagcttgt cttggtccat tcctttccca
     1321 ttttcaacga tttttaacac ataatggttg gttgtttcag ttgcatagac agtcaaacga
     1381 ttttcctttt ttgatgaatc gaagccgtgt ttaatataat tttcaacaaa tggctgtaaa
     1441 gaaagttttg gtaacattaa ttgatcaata tgatcaatca gctgatagtc aaagttatgt
     1501 cgataacgac gttgatgtag ttgcaaggag aggtcgatat taattaattc ctctttcaaa
     1561 tcaattatgt cgggctgata tgtattattg cgatacaact tgctcaataa atatatactg
     1621 tcgctgattt cttcgtcatc attcaatatg gcagtcattc gaataaattc caaattatta
     1681 aatagaaaat gaggctcgat ttgatattga agtgctgcat actttgcttc ttgcttcaac
     1741 caattatttt cataaagtgt tttttgatat gtagcattat ctaaataaaa ctgattgata
     1801 tgcatagcga tttctttcaa atcaccgact ttgttttcga tattgattga cttgtagtct
     1861 cctgtagatg aatgatttaa ggaagtaatg atttcctgat actgctggat atagttacga
     1921 aaacttctat ttaataattg atgaatagca agaataaaca aactcattaa taagaaaatc
     1981 atgattgcca gacctaataa ggtttgtgac gaataatttg gattttcata tacttgtaat
     2041 gtgtggttac cgatagctat ctgttgcgtt tttttccatt tagaaggcga gatgtcttca
     2101 gataaattga cggaatcaat accattagac aaagaataat tgtaatgtgt tggaaagaga
     2161 ctctttttta gcgcgttgat cgaaacgtct agaatcaatg tagcatcggg catgtcctca
     2221 ctaatgaaac ttaatgaacg aataaaagaa attttttcgt tttttgtagg tgcgtaggca
     2281 cttaagtatt tttcgtaatc taatgagata gatatatttt taacttcatc atcttgcaca
     2341 agttttttta agttatctac taaataggta ggggagcgat cctttgacaa aatatcttcc
     2401 atgtattcag caggtgatga gaggtagtag tcattaaggt tttcttggaa atcataattt
     2461 tgatagatct ctgtcacgat accggagaga atacgattat atgtttccat atagtgggtg
     2521 atttgctgaa tgttttgcag gttttgattt ttttcttttt caatcacatt gatataggaa
     2581 aagaacaatg tgaaacaggc aagtaaagta ataaaaaaaa tcaatatgta tgtaaaacgc
     2641 ataataactt ttgataaatc gtttttcatt caaagtcctc attttgataa tctctaattt
     2701 tatgaatcat tatagcacat attacaagtg atgtaaacgc tttgcaaaca gggctactta
     2761 tgattgtgaa tacagtttcc gtttaaaaac tattctattg tagaggttta ataaactaaa
     2821 aataatgtat aaaaaattaa tgtaagcgat taaccaagtg ggtataatca aggaaacgct
     2881 gagagaggag agacaaaatg aagctaaaga aagggttatt aggaaatcta aaaaaaaacc
     2941 gagcattgtt tattatgctt ttaccaggtg cgttatggtt atttatattt tgctatatac
     3001 caatgtttgg agttgtaatc gcttttaaag atttcagaat ttcatcaagg ggattcttta
     3061 caagtttgtt caacagtcct tgggttggct gggaaaattt caaatatctc tttgcttcgc
     3121 aagatgcttg gatcattact cgcaatacgg ttgtttataa cgttgtaggt attttgctaa
     3181 gtactttttg tacagtgtca ttagcaatca ttctgagcca aatggcgaac aaacggttat
     3241 tgaaaaagat gcagacattt atgatttttc ctaatttttt atcatgggta gtcatcagtt
     3301 actttgttta cgcatttttg agtacggata aaggattaat taatatttta ctaagcaatt
     3361 ttggtgtgga acccataaat tggtatagtg aaccgaagta ctggcctttt attattgttt
     3421 ttacgagttt atggaaaggc gtaggctatg gatccatcat ttattacgcg tcaattatgg
     3481 ggatcgatac tagttattat gaagctgctt tagttgatgg tgccagtaaa tggcaacaga
     3541 taaggaatgt agtcttgcca catttaagat cgattattgt cattatgttg attttgtctg
     3601 ttggtagtat ttttcgagct gattttggat tgttttatca attgccaaga aattcggggg
     3661 ctctttatga tgtgacgaat gtattagata cttatgtcta ccgtggatta atgaatagcg
     3721 gagatatcgg aatgtctacg gcagcgggat tatatcagtc atcagttggc tttgtcttga
     3781 ttttgattac taataagata gtcaacaagt tagatcctag ttcagcacta ttttaggagg
     3841 aatcaaaatg atatcaaaat taaaacggca gatgtacttt tcgctgccag tcgaacggtt
     3901 tttcacaata ttactaatct tatttactct tagctgtatt tttcccttta ttttcgtaat
     3961 cgtcatttca ttaacagatg aaaaagcatt acaactcaac ggttacgaat tattgccggc
     4021 tcaatggagc actgatgcat atcaatactt aatacaagat ggcggacagt tacttcggtc
     4081 attaggcgtt acaattatga ttacagtgat tggaacgttg attactgttt ttatgacggg
     4141 aacctatgct tatgttttat caagagcctc tttcccttac cggaagttct ttacttttta
     4201 tctttttttt accatgttat ttgcgggagg gatggttcct tcttacttag tgatgacgaa
     4261 gatgcttggc ttaaaaaata caatttgggc tttgattcta cctcttgctt ttagcccata
     4321 caatgtcatt attttaagga cattctttaa gaagtcgatt ccagaatcga ttatcgaatc
     4381 cgctaaaatg gatggctgta gtgagtttcg agtttttttc caaatcgttt tgcctttagc
     4441 gatcccaggg gttgcaacaa taggtttatt ttcaagctta ggttattgga atgactggtt
     4501 caacgcattg ctgtatattg acaccaacaa attgattcca ttgcaatatt tgttaatgaa
     4561 gattcaaaat tcaatggaat tcttagcaaa taataatgac attacattag cgcagcaaca
     4621 agcaattcaa aatagccttc ctcaagaatc aacacgaatg gcaatggtcg tagtagctac
     4681 tttaccgatt gcaattgttt atccgttctt ccaaaaatac tttgttcaag gattgacgat
     4741 cggtggagtg aaagaataaa aaagaatagg agatgttaaa agatgaaaaa gaacatgcag
     4801 aaatatttga ttggtggtgc ttctgtacta acccttggcc tattaggtgc ttgtggtagc
     4861 agtaatggcg aagcaaataa tgaaacagga accacaaaag ttacttggta tcaggttgga
     4921 gaagaaccca aagattttga tgaggtcatg acaaaagtca acgaaaaatt aaaggaagaa
     4981 atcaatgtag agttggatat gcggctgatt ccggacgggg attatcagca aaagcttggt
     5041 gtgatgatca attcaggaga agaatatgat atttgtttcg tcaatggcac agattatgtg
     5101 aattatggga acaaaggtgc tttcattagt ctcaacgatc atttggataa agagttgaaa
     5161 gattatgcag ctgaattgaa tgaaggattt attgaaggtg gggcaattaa tggcgaaact
     5221 tatggattgc cagtcaataa cgatattgcc acaacggaat attggacctt taataaagat
     5281 ttggtagatc aataccaatt ggatgtagag aatgttgaag gtgtagaaac attggctcca
     5341 gtattagaaa agtttgcaga aaaaaatact gatacgaata ttacgccaat cggtatgacc
     5401 cgtaccagtg tctgtattcc ttatgacaat attatcggtg gtggtgtacc gctagcggtt
     5461 gcatttgaag gcgatacaca aacagttgtt aatacttatg agactccaga aatgatgact
     5521 tatttgaagg ctatgcatga gttttataaa aaaggatata ttttgaaaga tgcagccact
     5581 gttacgacag atccatttcg aattgatagt aaaaactggt ttttaggtcg taactcaagt
     5641 ggttatcaac cattagcaca aaaaaaatta gaagctctag cgggacatgg attaatttta
     5701 aaaccagctg gaaaagcttt taaaacgact ggatttgcaa gagtggcaat gcatgcggta
     5761 tctgcttcaa gtaaacatcc ggaagcagcc ttgaaattgt tgaattatgt gaataccaat
     5821 caagaagtag cggatttaat ggtttatgga attgaaggaa aacactatga tcgcaatgaa
     5881 gatggttcga ttactttaac aaactctgat gattattatc aaggcgcttg ggcattggta
     5941 aatacttatc tagtttcaaa aacttcaccg attacagaag aaagtaaaaa actagaagaa
     6001 gaaagtaaaa agtttcaaaa tgaagcaccg atgtctccta ttttaggttt ctcttttgac
     6061 gcttctgatt ttcgaacaga aattgcaagt gtacaaaata tcgtgtcaca atatgaagta
     6121 cttttaagct cgggaacagt tgaccctgaa caatatgtac cagagttgtt agaaaaacta
     6181 gaaaaagctg gattggataa aattcaggca tctgctcaag aacaatacga tgaatggcga
     6241 aaatctaaca aataattttt tactaaatga aaagaataga ctaagcagtt agtgggggga
     6301 ttcaaatgaa cgaaaacaaa tggaccaaag gatttaattg ggtttttcaa ttgttgttac
     6361 taaactttac ttattttata ctaatttgtt taggatttgg aattttaggt tttttccctg
     6421 ctacgatagc tttgattgct ggcgttgtga atcgaaaaaa atatggcttg caagagtctg
     6481 tgaagttcat gagtaaaatc tataagaaaa attttgttag atataatcta tattgctggt
     6541 cagcaacttt gttgttggcc ggcaatatta ctacgatctg gctttgttgg cagcaaagcg
     6601 caccattgtt ttatttatta agtacattat tatcggtaat tacactgttc ctagtctttg
     6661 caatgttgaa ttgggtcgtg atcaaaagtc ttgggttgtg gatgatggga gaccgactga
     6721 ttgactattt gcttttcaca gcaacgatgt tgccaacggt agtcatgcaa ggcttggtgc
     6781 ttggaattta tttccgaata ttgatgattt tgcctgtgat ttttctgttt ggcggaacag
     6841 ctgtaacggt aatattgatt agtaaattaa ctgaaagagc gttagcaaag ttgccttgtc
     6901 agatacaatc gcactaattt tttcacaaaa aggagtattt ctttctatga agttaactga
     6961 taaacaagta gctaatatag cctttatcca gcaacttgag attccattga cacaaaaaga
     7021 tgaagaacaa atagacttga tttttactga cacaaaggct aatgtgcgac aaatcaagat
     7081 tactaaaaat gagcagcaac gaatttgctt gtcttattca catgaagact tgctgtatcg
     7141 aggattgttt atctgccttc cacgtttttt agctcaagga ggagagatgc catacattga
     7201 agaaacctcg atcattgaga ccagttattc attagatgct tctcgtaatg gcgtgatgaa
     7261 taaagagact ctaaaaaaat tcatttctta cttagcgttg atggggtaca acacattgta
     7321 tatttacaca gaagatacct atgaagtaga agaccttcca tattttggtt accttcgcgg
     7381 agcgtataca aaagaagaag tgaatgaaat tgttgaattt gcttctggtt ttggcataga
     7441 agtagttcca tcgatccaga cccttgctca cttgacccaa tttttgaagt ggtatgctca
     7501 acaagaactg atggatgatc aaaatactct actgatcggt gaagaaaagg tttatgcggc
     7561 aatcgaaaag atgattattg cctgcaaaga aatgtatcag acagagcgta ttcatttggg
     7621 aatggatgaa gcctatcaag caggtctggg acgatattta agcctgtatg ggtattgtga
     7681 tcggacaact ttaatgattc aacatatgga ccgtgtgtta tcaattgttg ataaatataa
     7741 gatgaaacca atgatttgga gtgatttcat ttataagcta ttagatgaaa ccggtaaaga
     7801 taggtattac aatcctgatt ttaaactcaa agcattagca gctaaaggat taccagcagg
     7861 attaacctat gttcattggg actatggttc agatgaagtt tcctcctatg agaaagtaat
     7921 tgataaccat ttgaaatttt gtgacattga tcattacgtt ttagctagtg gtgcccatat
     7981 ctgggggaga ctagccccga atcatgggaa aacagaagca attgtttctg cagctgtaaa
     8041 agcttctaaa aataaaggtg tacgttctgt gatgttaact acttggggag atgaagggca
     8101 ggaaacagag cattggcatt ccttaattgg ggcattaaaa ttagcagatt atgtctattc
     8161 tgaagaagag tggaatgatt ttgaggaaat ctacgatcgt ttattaggaa caggtagtta
     8221 tcaattaatg tccgaattac gatatttcga tgaagtggat accattacaa ataacaactt
     8281 acaaatggct tccgtgagta aactgctact ttggcaagat ccgttagttg gtatatatga
     8341 ctttcatgta gccgctcata acaaaattgc cactagaaca attggtactt attatcatga
     8401 gttagctcag aaattagata tgaataaaaa ttggaatcca aaacaaccaa ttttaacaac
     8461 tattcgtcaa cgatatatac ttttagctga aattttagca caaaaggcag aactaggcat
     8521 ctatttacaa cactgctgtc tggtagaaga ccttgcagga ttaatggaat tagcgaatcg
     8581 gtgtgaagca ctagccaatc gagtaaatga attggcatct attcatgaaa taatttggta
     8641 tcaaacatac aaaacctatg gttgggaagt attagaacaa cgatatgctg cattagctca
     8701 tcgtctgaaa accgttgcgc gtcgtattaa aaatcatctt caggggcaag atgccctaga
     8761 ggagcttttg gaagaacgat taccgttttg tcaatatcaa gtgccgcttg aaatttctgg
     8821 ctttaattat cggcaaagct cggtgtcagg ctacaattaa cgaaggaatc ttcccgttga
     8881 acggaccact tgctcatcta atgggaaagt ggttcgtttt ctattcataa gtagcttacc
     8941 actaactctt ttattaagaa gagaaaaaaa gtaggaagtg agaaaatatg ttatcggact
     9001 attatcaaaa tctgtcagtt ataagtgtca atacagaacc attacgtaat tattatattc
     9061 cttatgcaag taaagaagaa gcaaaacaag agttagaaag ggaaaaaacg agccagttta
     9121 tcgatctcaa tggtacatgg gattttcagt attatccttc tgtccaacag ttaccggaaa
     9181 atttttttca agaggccttt actgaaagct atgatagaat ttctgttcct ggagtttggc
     9241 aaacagaggg atttgactta cctcagtata tcaatgtatc ttatccaatt ccttttgatc
     9301 cgccttatgt acctaatgat accccttgtg gtgtatatcg gcgtcagttt caaattgagc
     9361 aaacagatgc acgcttttac ttaaatctag aaggagcaga tagtaattgt tttgtttgga
     9421 taaatagaaa ctttgtaggc tatgaccaag tgagtcatag tacatctgaa tttgatgtga
     9481 ccaagtattt acaggttggg gttaatgaaa ttcaaattct agtgatgaag tggtgtgatg
     9541 gtacctactt tgaagatcag gataagtggc gaatgagcgg tctatttcgt ccagtgtatc
     9601 ttcttcaacg accaactatg catctgacaa gttatcaact acggacgttg actgatccta
     9661 aaaaaggaag cggaacatta atggtttctt ttcaacgatt tactcctatt gaagtatcgt
     9721 tacctatttc ttacgaatta ttccatcctg atggacgtct catagcgaaa ggggagtgtt
     9781 actcggaaca acaaataaat ttggatgttg aagattgcct tttttggaca gcagaaacgc
     9841 ctaaattata tacattactt ttaacatgta atggtgaata ttttaaagaa aaagttggct
     9901 ttcgagcact gacgatcaag aatcgtaagt tattgctgaa cggaacacct attaaattat
     9961 atggagtcaa ctatcatgac gtgaatcagc aaggcacgcc agtgatgacc cgagagcagt
    10021 tacgtgcaga tttgttatta atgaaaaaat taaatgtgaa tactgtacga acggcacatt
    10081 accctaaatc accagatttt tatgaattgg cggatgagat tggtctgtat gtaatcaatg
    10141 aagcagattt ggagtgtcat ggtgtggtta atctatatga tactgaagcg aattatcaac
    10201 taattgctaa tgatccgaca ttcgaaaagc cgattatgga ccgagtgatc cgttctattc
    10261 ttccttatca aaatcgaagc tctatcatta tgtggtctat ggggaatgaa tctggttatg
    10321 gagtaaattt tgaaaaagct caagctttag cacggaaatt ggatgattca cggttgattc
    10381 ataatgaacg ctcgattgaa ccagttattg accaagtgaa tgattttagt aacttggatg
    10441 ttattagtca aatgtatccc ccactggaag atgttcatcg tcattgtcaa agaagtgata
    10501 ctgaaaaacc tttttttatg tgtgaatata gccacgctat gggcaacggt cctggagatc
    10561 ttaaaacgta cttcgatttg attcaacagt atgattcctt tcttggcggt tgtgtctggg
    10621 agtggactga tcatgcaatc aatagaggaa ctttagaaac gccatcttat ttatacggtg
    10681 gtgattttgg tgaaaaatat cacgacggca atttttgcgt agatggttta ttgaccccag
    10741 atcggcaatt aacagtaggt gcattggagt accgtaatat tcattcgcct attcaactga
    10801 ttgctgtaga tgaggagctg caagaagtaa ccttgagcaa tataactgct tttacagccg
    10861 gttcagcatt taacctttct cttgaatggt atatagatgg aaaaaaaaca gtttatgaag
    10921 taatgaatcc tttcatactt gagcctggca aaagttgttc attgcccatc acagtaccta
    10981 caacgattca tggtgctcat acggttgctg tttcgctatc tgtttataaa aatgatgagc
    11041 tttatggaca gcaacagctc tttcttaaaa aagaaaaaat gagattgcca agaactgctc
    11101 atcataatga tgcaccacaa atttcagtaa aaggcacaga gctgataatc aaaggagaat
    11161 cctttattta tatatatcaa ttagcaaaag ctgcgttcac ttctgtaaag atcaatcaaa
    11221 aggaactttt ggaacgtcct actcaatttt caatttggcg tgcaccaacg gataatgatc
    11281 gtaacttagt aaacaaatgg aaagcagctg gatatgctga aatgtcttta gaaatcagaa
    11341 aaaaagactg gaaattcgta gatgatcatc tcctgatcga aacgtcttat cgaggagtta
    11401 ttccaggatt acagccaatt tttacaatgc acatacagtt gaaaataaat ggtgcaggcg
    11461 taatcaacca aaaagtatca gtggagaaaa atccgatttt tccagatttc cctcgctttg
    11521 ggatggactg gccgttgaat gatacttttt cgaaggtttc ttattgtggc tatgggccgt
    11581 atgaaagtta tcctgataag cactatgctg cgatgtttgg tatttatgag atggagattg
    11641 aggaatttga tcagccatat atcaaaccac aagaatatgg taaccgctgg caatgtgatt
    11701 gggtatcaat caaaaatcat caaggatcta ccatgacggt ttatggaaac tctaattttt
    11761 ctgctctgcc ttatagtcaa gctcaattaa ccgaaaagtc acaccattat gaattaataa
    11821 aagaaaatag aacttggttc aatttaagtt ataaacaaaa tggtatcggc tcacacagtt
    11881 gcggtcctta tttagaagat cgtttccgtt ttatggaaga tacttttgac tggaatatag
    11941 tgattacttt caaaggagaa aatagatgaa agaaacaaag atcaatcaag tcaaaaaaaa
    12001 attatctcgt tatcaagaag ttgtttatcg aaaccattgc ttaaaatggt caaaaaataa
    12061 gctgaatgtt cgttttaatg gattatttta tcgtttcgaa cagttagaag aagtctggaa
    12121 accggtaacg aatttccaat catgtcctgt acagctcata aatgatccaa aagaaacata
    12181 cgatttatgt ataacacacc tttataacca gcatttgaaa aatgacggat tttatttaaa
    12241 ggttactaat gacaatcaac ttacgattga atcagcaaac cttcgagggt ttcaatatgc
    12301 tatggaagcc ttattagaga gttttttttc ggtaggagat caattattat taccggaatt
    12361 gattctgaaa catgaaccat cagtgaaaat tcgaggaatc attgaagggt tttatggtat
    12421 cccctggaca catgaaatgc gcctctctct tttttcatat atgaaagata accaattgaa
    12481 tacttttatg tatgccccta aagatgatga attattacgt aaaaaatggc gtgaactcta
    12541 cgatgcgcaa gaattagaca aatttaagga gcttttatct gctgctgagg ctagcaatat
    12601 cgatttttgg tacttaatta gtcctggaaa tgatattgat attacgtgtg aagaagacat
    12661 tcaagttctt ctgaaaaagt tagaacaact gattgagctt ggcgtatttc aatttggtct
    12721 attaatggat gatattgact atcaattaaa aggtgctgct aaacgccgtt ttcgagagcc
    12781 agcattcgct catgcctatt tagtgaatcg agtagaggaa tatcttagca ctcggttgtc
    12841 caatcatgaa ttagtcattt gtcctacaga atatgataat cgtcatggtt ctcgctattt
    12901 agcaacgtta agtcaagaaa taccagaaca attaccgttc ttttggactg gcccaagcac
    12961 gttagcggcg tcaatttcta cagaagagct gcaggaaatg gcttctgtct atcaacggcc
    13021 gatgctgatt tgggataata ttccggtaaa tgattattta gaggataaag aactattgtt
    13081 catgagtcca tatgagaatc gaacaccgaa tctttctaag gagagatacc aggttacagg
    13141 agtagtttcg aatccaatgg ctcaattaga agcttcaaag tttacgatca atagtatggc
    13201 taattactta tggaactgtg agcgttttga tcctttggaa acttggacgt cagtcgttga
    13261 aaaagtagtt ggaagtaaac tacaaccagc ttatcttacc ttaaccaatg cttttccaaa
    13321 tcattacaca agcagtctgc tttcagatga agaattactg ctagccaaag acccagaatg
    13381 ggtcacaaaa gaaatggctg ctttagtcca agcagtaaaa tggatccagc aagaacaaca
    13441 tacatctaga ttgagaacag aattggagcc ttggttacaa gcaatcacag aaagttggac
    13501 tttctggcaa gaatggcaat taaaaaaaga tccaaaagaa gcagaagaat ggttggctaa
    13561 gaaaaaaaca caccgcatag gacgtgattt agtaactgcc catttagagc aaattatcaa
    13621 aagttagata tcttaaaaaa taaaaagcaa gaaaaaggag aacaaaatgg tcaagagatt
    13681 aattagtgca aactacagtg atgtcaagaa aatgacgtca aaagagttga agcaatccat
    13741 caaagcgagt gaaggacggg tgattgtttg tgaaacggtt gctaatactc ttccacaatc
    13801 tggagaaatt acgaatgcag aagttgcgtc agcatttggt gcagatttga ttcttttaaa
    13861 tggtttcgat tgttatcgtc ctgcagtttt tggactacca aatgtgacga ttgagaaggt
    13921 ttttgatcta cagcaaacga ttgaaaaccc gattcctaaa ttaaaagaac tagtcggtcg
    13981 tcctgtggga attaatttag aacccattgc ccctaaaaca aatatgatga gtgagaaagt
    14041 agaaatcagc caaggaagag caagctctgt tgaaacgata caaaaagcag aagaactagg
    14101 agtcgatttt atttgcttta ccggtaatcc aggcactgga gtaaccaatt tagaaattac
    14161 taaatccgta aaaatggcaa aaaagtattt ttcgggattg attattgctg ggaaaatgca
    14221 tagcgccggc tcagaagagc cagttgtgaa taaagaggcg attatttctt atgcggaagc
    14281 aggcgcagat attttactag ttccttcagt agggtctatc ccaggctttt tagagcaaga
    14341 cctaattgaa gcggttcggt tggcaaaaaa atatgatttg ttaacgatgt ctgcaatcgg
    14401 tactagtcag gaaagtgccc gaccagaaac attgcggcaa ttagccatta tgaataaaag
    14461 ttgtggtgtt gatttacaac atattggaga tgctggatac gggggattgg caccagcaga
    14521 aaatattttc gaaacctcgg ttgcaattag aggaatgcga cataccatca actgcgttag
    14581 ccgctcgatc aatcgctaac aaaattaaat aaaaaatggt taaattgatt atttaagtgg
    14641 gaacatactt gagatatcag ctaacttctg tcactttaaa aaagggactg tgacagaagt
    14701 gaaaatagac aaaaaatatc cgaacaagag actaggatag cagaaaaact atcgttttgc
    14761 aacgaggacc aactggaccg taggagcaat tctcttattg ttcggatatt tcgcttgttc
    14821 aaggacttat gtctgtagtg cccttttttg gtaggaaaaa tggtagggaa ccatatcatt
    14881 tttaatgaaa atcaagcatt tcaaaagttg aaa
//
LOCUS       DACDXE010000072        14805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.072, whole genome shotgun sequence.
ACCESSION   DACDXE010000072 DACDXE010000000
VERSION     DACDXE010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.072"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..560
                     /locus_tag="IWM23_002261"
     CDS             <1..560
                     /locus_tag="IWM23_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAP9884870.1"
                     /translation="KNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTKH
                     IFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTAH
                     KVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEV
                     GYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            760..1074
                     /locus_tag="IWM23_002262"
     CDS             760..1074
                     /locus_tag="IWM23_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9884871.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1095..1472
                     /locus_tag="IWM23_002263"
     CDS             1095..1472
                     /locus_tag="IWM23_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9884872.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1482..2255
                     /locus_tag="IWM23_002264"
     CDS             1482..2255
                     /locus_tag="IWM23_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884873.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2277..3680
                     /locus_tag="IWM23_002265"
     CDS             2277..3680
                     /locus_tag="IWM23_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9884874.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3862..5046
                     /locus_tag="IWM23_002266"
     CDS             3862..5046
                     /locus_tag="IWM23_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9884875.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5043..5333
                     /locus_tag="IWM23_002267"
     CDS             5043..5333
                     /locus_tag="IWM23_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884876.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5330..5551
                     /locus_tag="IWM23_002268"
     CDS             5330..5551
                     /locus_tag="IWM23_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884877.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5593..6372
                     /locus_tag="IWM23_002269"
     CDS             5593..6372
                     /locus_tag="IWM23_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HAP9884878.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6439..6933
                     /locus_tag="IWM23_002270"
     CDS             6439..6933
                     /locus_tag="IWM23_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9884879.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6989..7627
                     /locus_tag="IWM23_002271"
     CDS             6989..7627
                     /locus_tag="IWM23_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884880.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7699..8094
                     /locus_tag="IWM23_002272"
     CDS             7699..8094
                     /locus_tag="IWM23_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9884881.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8078..10531
                     /locus_tag="IWM23_002273"
     CDS             8078..10531
                     /locus_tag="IWM23_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9884882.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10528..12555
                     /locus_tag="IWM23_002274"
     CDS             10528..12555
                     /locus_tag="IWM23_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884883.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12552..13574
                     /locus_tag="IWM23_002275"
     CDS             12552..13574
                     /locus_tag="IWM23_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9884884.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13591..14520
                     /locus_tag="IWM23_002276"
     CDS             13591..14520
                     /locus_tag="IWM23_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9884885.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
BASE COUNT         5030 a         2469 c         2928 g         4378 t
ORIGIN      
        1 taaaaaatga atttggaata gaagttaaag attattttat tgacttagat ttattacagc
       61 taataaacag tgtgaaaaga atgtatagtg gagacgacaa cggtgttgat ttatacaaat
      121 ttgttaccaa acatatattt gttaatctta atattgacga gaaaaattac ccgtctattt
      181 ttaagcacta tctatttttt attaacgaag tagaagatcg gataaaaaat aattcattat
      241 ctagtagtgt atctagtttt aagaagatgt ttaaatctcg agatggtatc acacttacta
      301 cagcacataa agttaaagga aatgagtatg atactgttat cgctattaat cttctaaacg
      361 ggaaaattcc tcattggaat gaaattttta atgtttctga taaaggaatt agttcagcga
      421 gaaaactact atacgtaatt tgttcaagag caaaaaataa tttatttctt ttctcagagg
      481 tgggttattt tacgagtaaa ggagacttgc tttatcccac tcaattatta gataaaatag
      541 aatttgatta taatatttaa aatacctagg tagtcgttgt tttaaatata gtaggaatcg
      601 gacgctcagt acggagcgtc tattttttta cccaattttg aaaggaagtg aacttatgaa
      661 aataaaaaat caagaatcaa aaggtcgctc tccccccttt aaaaccatca cacaaataag
      721 attcattcaa acataaaaac agaaaggata ggtaaaaata tggaacttaa atatgtcatt
      781 cccaatatgg aaaaaacatt tggacaatta gaatatgctg gtgaagggaa cattgaacaa
      841 cgtcgaataa atggcagacc aaccattctc tctcgtagtt acaatttata ctcaacgatt
      901 caacgagcag atgatattgt tgtcattctt ccctccgaag ctggcgagaa acattttgaa
      961 tccgacgaaa aagtcaaact catcaaccca catattaccg cagaaggtta taaaattggc
     1021 aatcgaggat ttaccaatta cattatgcac gcagacgata tggtgaaagc ataagaaagg
     1081 aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat tgaaaaagac cagacatttg
     1141 gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat ccagaatgaa gatggtacgg
     1201 tttccgaaga aattaaagaa cgtacctatg acttaaaatc aaaaggacaa ggacgcatga
     1261 tacaagtcag tatccccgct tctgttcctt tgaaagaatt tgactacaac gcagaagtgg
     1321 aattaatcaa tcctgtggca gataccgtag cgacggctac ttttcgaggg gcagacgttg
     1381 attggtacgt gaaagcagac gatcttgtat taaaaggaca gacaaaacct catcagaaat
     1441 ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct tatgacaaac acaccattga
     1501 ttttgaaaga aattccgaag gatgaagcca tttcttttat tcggcaatac cattattcaa
     1561 agatactccc tagattgtgt aaatactttc tagggatttt ttcagaagaa aaattattag
     1621 gcgttgtgga attaggctgg ggtacgcaac ccctacaaac catacgcaaa ctctttcccg
     1681 acagttccct tcaaaccaca gattatttag aaattggaaa gatgtgtttt cttccagaaa
     1741 tgaatcaaac caattatttt ggcagtcaag ctctttcagc tctaattaag tggttgaaag
     1801 aacatacgga ctgtcatttt ttatatacac tggcagatgg aattgaggga aaatgtggct
     1861 atgtctatca agcgtccaac ttcttttact gcgggtattt caagacaagt gtctatcgtg
     1921 ataagcagtc atgggaaaag atacaccctc gaagtgctcg ccttttatta gaggaaaatg
     1981 ctcgctttga acaggtagag aaaaagcatt ggctttccca ggcattttgc gaatacaaag
     2041 ggattgagaa aatcaatgga cgcatgtttc gctatctcta tccgttgaca aaagaagcga
     2101 aaaaactgtt aggacacact ctctatcgac gtcattatta tccgaaagaa aaaaatttac
     2161 gctttgaaaa gcgaatcgct tatcagaaat atgaagccat ttctcaacca acttttgata
     2221 aacaggctcg tatctacaat acacaattat tttgataggt ggaaaggagg atttttatgc
     2281 cattgcttca tgacagagga gagcgaatca aagccaacga taagcacttg gtctatcatt
     2341 ttgtactaat ttcattgctg gtagtttttc tagtcgtcct actccctttt cattggagag
     2401 cgttacaatc ggtgaactgg caagcagttc agttagatac atttttctat caacttcata
     2461 cgccatactt ttggagcagt attctcactg ctctttttgt atgcggactt atcagctggc
     2521 tttattatca ctatcgtata gattctataa aacagttaga gcaccgtcaa aagttagctc
     2581 gtatgatttt agaaaatcat tggtatgaag ccgaagaaac tcaatcagag ggattcttta
     2641 aagatttacc cagcaagaaa accaaacaac gaatccgcca ttttcctaaa atatattatc
     2701 gaatgaaaga tggactaatc catattcatg tagaaatcac cttagggaaa tatcaagatc
     2761 agcttttgca cctagaaaac aaactagaaa caggattgta ttgtgaatta gtggaaaaaa
     2821 cgttgaaaga ttcttatgta gagtatgtcc tactttatga tactattggc aatcgaattg
     2881 gcattgaaga cgtggtagct caagatggac gtgtcaaatt gatggaatcg gtctattggg
     2941 agttcgattc tctccctcac atgttaattg ctgggggcac tggtagtgga aaatcctatt
     3001 ttattctcac attgattgaa tctctcctgc atacaaatgc aaagctttat attcttgacc
     3061 ctaaaaatgc tgatttggca gacttagggg cggtcatgaa taatgtctat taccgtaaag
     3121 aagatatgtt ggcatgtatc aatcaatttt acgaagatat gattgatcgc agtgaaacca
     3181 tgaaacaaca ccctaactat aaaacaggag aaaattatgc ttatcttggt ttgtctgcca
     3241 attttcttat ttttgatgaa tatgtggcaa tgatggatat gctaggtcgt gaaagtacca
     3301 ccgttattca taaactcaaa caaattgtaa tgttaggacg acaagcaggc ttctttttaa
     3361 ttcttgcctg tcaacgtcca gacgcaaaat attttagtga tgggattcgg gacaacttta
     3421 acttccgtgt ggctttgggt cgtatgtctg aaatgggctt tggtatgatg ttcggaagcg
     3481 atacacaaaa acaatttttc ttaaaaccca tcaagggaag aggttacgtg gatacaggaa
     3541 aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa aaggtacgat ttcttaggag
     3601 aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt accgcacgaa gtgaaaggta
     3661 caggttcaga cttgctttga ttggtgtggc gttagccacg ccaatccttt agccccctct
     3721 atctaacagg ggggcacaaa tacaactgaa aacaggcaat tctcatcaac caatgttgat
     3781 ggtacaaagg attgcggaaa atatgaaagg tgtaaactca agggtcatag tttacatctt
     3841 ttttagattg gaggtttgcg aatggaaaac gaaaaaattt ggattaagga attgaaagaa
     3901 aagcgactgg cttatggcgt ttctcaaaat aaactggcgg ttgcttctca tattaccaga
     3961 ccttacctat cggatattga aacaggcaag gcagtcccta ctcaacaggt gaaagaagat
     4021 ttactgaacg ctttggaacg cttcaatccc gataacccat tggagatgtt attcgactat
     4081 gtacggattc gattccctac caatgatgtg gctcaaatta tcggtgaggt tttacgtttg
     4141 aatatggatt atatgctaca cgaagacttt ggctattatt cttatcccga acactatcgt
     4201 ttcggtgata ttgttgtcct agtctctcat gatgtaagta aaggtgtttt attagaactg
     4261 aaaggaaaag gctgtcggca atttgaaaac tttctattgg ctcaacatcg gagctggtat
     4321 gatttctttc aagattgcat ggaacataaa ggtattttca aacgcttgga tttagctatc
     4381 aacgataaaa cagggatttt gaatattcca gatttggcaa agaaatgtaa acaggaagaa
     4441 tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg gtgaattggt tcaccgtgat
     4501 gaaaaaccat acatgggaaa cacactttat attggctcat taaaaagtga agtctatttc
     4561 tgtatatatg agaaagatta tgagcaatac gtgaaaaatg atgttccctt agaagacgca
     4621 gaagtaaaga atcggtttga aattcggtta aaaaatgacc gagccaccca agccatgaaa
     4681 gatttattgg cacatcaaca ggcggaaaaa acagcctttg aaattatcaa tcgctatatc
     4741 cgctttgcag ataaagacga cactaaacgt cggagtgatt ggaaaactaa tgaacgttgg
     4801 gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa ccacacagcc agagccttat
     4861 tcttttgaac gaacattgaa ctggttacat catcaagtcg cccctacatt aaaaatcgca
     4921 agcattttag atgttctcaa cggtacaacc attatctcca ccatgattca ggaagccaaa
     4981 ctgacagaaa aacatgagaa attaattgaa caacaacatc tagccatgga ggatttgatt
     5041 acatgagcct tttaacaaaa gaattaaaga agttagggtt tcagtgtggc attgagtttc
     5101 aagcgtatat acaaaataca gggaaatata cgtctctcat tattgaggga aaacggcaag
     5161 ctggtgatac catctatacc tatgattttt acaaggtgag attttataat aattatacca
     5221 atcgagtaac cgtctatgga gagcatttaa ccccttttca attattaagg cgtgtaaaaa
     5281 gctatatcta ttatcgagaa aaatatctta aggaaaggag gacaatcaca tgaattttgg
     5341 gcagaatttg tacaactggt tcttggataa tgcacagtca ttggtattgt tagcgattgt
     5401 tgtcattgga ctgtttttag gttttaaacg agaattttca aaactcattg gcttcttgat
     5461 tattgctcta atcgccgtcg gacttgtgtt caatgcagct ggcgtgaaag atgtgttatt
     5521 aaacttattt aaccgaatca ttggtgctta acagatattt ttcatgataa aataggaagt
     5581 atatattttg ttatgaaagg atttatgaat atgtttacaa atgatcaatt taaatctctt
     5641 ttaattaaaa cattagaaag aaagagctct tcaaattatt atgaaggagg taacgagatt
     5701 gtttctaaaa tgaaaatatc agaggtaact ttagatgaga ctgacgattt tacttattat
     5761 aaaggatata aaaagggctg gaatacgctt tgcacatatc ttaaaataag agttcctttt
     5821 gatgatttag acttttttga aagtcataaa gatttgatta cccagacagc aagctccata
     5881 tatgataaac aaggtgataa tgtattagtg gatactattt tagtaccact tccagagaat
     5941 tacgaagtaa ttaactttag tcagttgaaa attagtgatg tggtttcaca agctattgaa
     6001 gacgctgagt cttttatgag taatggtgaa tatcaacgtg cctttgacag agttcacact
     6061 gcttttcatg gatatttaat tgaaatacta aaaaaatatg aaattacagt tcctagagac
     6121 gaaaatctat ctaaattata tagtaggata caacaattaa ttgaaaaaga aattcaacct
     6181 actgaacttg ctgatatagt gaagacaacg ataaggtcat caaatggaat gattagctct
     6241 cttaatgaag caagaaatag gcattcactt gcacacccta atacaaatat tattggaaag
     6301 agagaagcaa aactaataat aggtatttca agtacagtta cagattatat tagcggatac
     6361 cttgataagt agcgtcatta actgattgct acttttttta tgactatttt tataaaaagg
     6421 atgtgataag aattggaaat gaaagtctat gtggcaaatt taggacggta taatgaaggt
     6481 gaattagtcg gagcatggtt tactccacca attgatgagg aagaaatggc agaaagaatc
     6541 ggtttaaatg aggactacga agaatatgca atacatgatt ttgagcttcc ttttgatgtg
     6601 gacgaataca cgccgattag tgaaatcaac cgtctatgtg aagccattca agaaatcgaa
     6661 ggtacaccta tttataatga actcaaagaa attcaaggca tgtggtttag tagcttggaa
     6721 gaattactgg aaaacaaaga ggatattcat tgttatagtg attgtgattc gatggaagat
     6781 gtcgcccgtt attacgtgga agaaacagga cagcttggtg aagtcccttc caatttacaa
     6841 aattatattg actaccaatc tttgggacgt gatatggaaa tagagggtaa ttacctagtt
     6901 acctctcatg gggtatttga atattgtcag taagtttaac aatccgtgtt ataattaaat
     6961 taataaatag cacggagagg tataaattat ggtaaatgta tttgaaaatc ttaaactatt
     7021 aaaactagac atgaaagata aaggctgggt gatagattcc ttttactttc gttacaaaca
     7081 gcaaaattat attgtacttg ttaagctatt tgaaaaagag gaaaaagttc cagaatatgc
     7141 tctgttaaaa ctagaatttt taaaggaaaa cgatttttca gatatgctag ctgtctatgc
     7201 taattcggta aaattatata cagacacgaa aacaataaga gaatattttg gtatcgaata
     7261 cagtagtaac ttgggagatg ttttatttca atttagccaa actcttgctc gatttattcc
     7321 gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa gccatgtgtt tctcattaag
     7381 tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt tcagttagaa gaaatccttt
     7441 aaagggaaat ggtgagctgg gaaaacgaag tccttttaat gacaataaaa cgagattgta
     7501 tagacccagc ttatatgaac gattagggtc agataccaat ttaagtttca gatattcaat
     7561 gaatccagat gatgaagaaa ctgatgaagg tattattgca aaatggacaa aaaataaaat
     7621 tgaatagtta aagcattgaa agagcaatca cctattttga ttgttctttt tctattttta
     7681 cggaaaggac gtgaagacat gaaaaaaata agaagttaca ccagtatttg gtcagtagaa
     7741 aaagtgattt atgcgattaa tgatttacaa ctccctttcc caattacctt tacgcaaatg
     7801 acatggttta tcgtttcttt gtttgtcgtc atactctttg gcagtttacc accactttct
     7861 ctgattgacg gagcactact caaatactta gggattccca ttgttctcac tgggttcatg
     7921 tgccaaaaga cctttgacgg aaagaaaccc tatggatttc taaagtctat cattacctat
     7981 gctctacgcc ctaaagtaac ctacgctggc aaacaaatac gattaaaaaa agaaacaatg
     8041 aatgaatcga ttacgattgt aaggagtgaa caactagatg gcatatccga ttaaatatat
     8101 ggaagataat ctcgtattta atcatgatgg cgaagtattc gcttattatg aattaattcc
     8161 ctataattac agttttttaa gttccgatga aaaaattcaa gttcatgata atttctgtca
     8221 attgattgcc caaaatcgtg atggcaagat tcatgctctg caactagcaa ccgaatcaag
     8281 tattcgagcc acacaggaac aatcgaaaaa agaaatcact ggacgattaa aagagatagc
     8341 ttacgaaaaa atagatgaac agaccgaagc gttaatttct atgattggtg acaatcaagt
     8401 agactatcgc ttttttattg gttttaaact ctacctcaat gaacaggaag tatccgtaaa
     8461 aaatgttggg aaagaaattc aaaaagcatt tgcggaattc tttcatggtg tgaatcacaa
     8521 attaatgggc gattttgttt ctatgcctat ggcggaaatc gaacgctttt ctaagatgga
     8581 acagttgtta gaaagtaaat tatccagacg ctttcagatt cgtaaattgg agaaagatga
     8641 ttttggttat attattgaac atctttacgg gcaaacaggt gtcccttttg atgactacga
     8701 ctaccattta ccgaaacgat acgaagataa aaaagtgttg gtaaagaaat acgatattct
     8761 caaacccact cgctgtttaa tcgaagaaaa tcaacgctat ttacggattg aacaggaaga
     8821 taccactact tatgtggcgt attttacaat taatagtatt gttggtgaat tggatttccc
     8881 taatagtgaa attttttatt accagcaaca gcagtttact ttccccgttg ataccagtat
     8941 gaatgttgaa attgtcgcca ataaaaaagc cttatctacc gttcgcaata aaaagaaaga
     9001 actgaaagat ttagatagcc acgcatggga aagcgacaac gaaaccagta ctaatgtggt
     9061 ggacgcttta gaatctgtcg atgaattaga aagtaattta gaccaaacca aagaatctat
     9121 gtacaaacta agctatgtgg tacgggtatc agcaaagaat ttggacgagc tgaaacaacg
     9181 ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc aaattagtac gaccttttgg
     9241 cgatatgcta ggcttgcata atgaatttat tccctcaagc aagcgatata tcaatgacta
     9301 tattcaatat gtcacgtcgg attttttggc tggtcttggt ttcggtgcta cacaaatgat
     9361 tggtgaaaat tctggtattt acgtcggtta caatctcgat acaggcagaa atatttatct
     9421 ccaacctagc cttgcaagtc aaggaattaa aggctctgta acaaatgcat tagcgtccgc
     9481 ttttattggt tcattaggtg gcggaaaatc attctctaac aatatgctgg tctattattc
     9541 agtactcttt ggcggacagg caaccattgt tgacccaaaa gctgaacgtg gtgaatggaa
     9601 agaaacctta cctgatattg cccatgaaat caatattgtt aatctcacga gtgagaacga
     9661 caataaaggg ttacttgacc cttatgtgat tttaaaactc ccgaaagatt cagaaagcct
     9721 tgccattgat attctcacgt tcttaacagg tatttccagc cgagatagcg agaagtttcc
     9781 tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa caacgagggt tgttattggt
     9841 gattgatgaa ttgcgaaaag aagatacgcc catcagcaat agtattgctg accatatcga
     9901 aagctttgtc gattatgatt ttgcccatct gttattcagt gatggcagtg tcacccaatc
     9961 cattagcttg gaaaaacaac tcaatattat tcaagtggca gatttggtct tacccgattc
    10021 caatagctct tttgaagaat acactaccat ggaattatta agtgtggcaa tgctgattgt
    10081 cattagtacc tttgctttag actttatcca ctctgaccgt tccattttta aaattgttga
    10141 ccttgatgag gcttggagtt ttttacaagt ggcacaagga aaagctctat ctatgcgatt
    10201 ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc gttactcaaa acgcggacga
    10261 tttactagat gaaaagatga agaataatat tggcttgaaa tttgcctttc gttccactga
    10321 cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg gacaaggaag atgaaaacaa
    10381 tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta gtttcagaca tctatggtcg
    10441 tgtaggggtt atgcagtttc acccgatttt tgaagagttg cttcatgcct ttgataccag
    10501 accgcctgtg cgaaatgagg tggaagaatg acacaaaaac aaaagaaatt tcttcactat
    10561 agttggataa cgctcctttg tgtgggcgtt attttacttt tattaggcac gctgggcaat
    10621 gcagtacaag cgacaggctt agttgatgaa accattgata caagcaatga atactccaag
    10681 tatggtttga atcaatatca gcttgattat tatgtggaca atagctgggg ctggcttcca
    10741 tggaattgga gtgatggcat aggacaatcg gtgatgtatg gattgtatgc cattactaat
    10801 tttatttgga caatttctct ttatctatcg aacgccacag gttatttggt gaaagaagcc
    10861 tatgccttag atttcattag tcaaacatcc caagcaattg gcgaaaacat gcagacgatt
    10921 gcaggcatta caccgaatgg cttttccagt tcagggtttt atgtcggttt cctccttctt
    10981 tttattctca ttgttggaat ctatgttgcc tatacaggat tggttaagcg agaaacgacg
    11041 aaagtgatac gagctgtttt aaactttgtc gtggtgttta ttctatcggc ttcctttatt
    11101 gcgtatgcac ccgattatat tgcgaaaatc aatgattttt ccaaagatgt cagtaccgcc
    11161 agtctgaata ttgggacaaa aattgttctt cctaacacgg atagtcaagg gaaagacagt
    11221 gtggacatga tacgtgacag cttattctct gttcaagtaa agcaaccatg gctattgctt
    11281 caatatggct ccactgatgt agaagcgtta ggggaagaac gtgtggaaag tttattgtct
    11341 accagtccag atacaaacaa tggtgaagat agagaaaatg tagtgattga agaaattgaa
    11401 gatcatgaca atacctattt aaccctaccg aagacgattt ccagattagg aacagtcttc
    11461 tttttgtttg tattcaatat tggtatctcg atttttgttt tccttctaac aggaatcatg
    11521 attttttcgc aagtgctatt tattattttc gctatgtttt tgccaatcag tttcctatta
    11581 agtatgttgc cgagttttga tggcatgggc aggcatgcca ttaccaagtt atttaatgtg
    11641 attatgacaa gggctggtat taccttgatt attaccgttg cctttagtat ttccactatg
    11701 ctttattcgc tgtcagcaag ttctcccttt ttcatgatta tgtttttaca aattgtgacc
    11761 tttgcaggga tttattttaa actgggcgat ttaatgagcc tattcacttt acaaagcaac
    11821 gactctcaaa acatgggcag acaagtgttt cgcagacctc gtcaaatgat gaatcgacaa
    11881 accagaagat ttcaacgcag tatgagtaag atttttcgtg gaaaagcagg gtcaacgaag
    11941 caaaagacaa tgacaacaaa gcagaatcaa accaataaag ctaaccatac tcgtcaaaat
    12001 gaacggacta caccaaagaa agaaacagca ctttccaatg tgaaaaagca acaaggcggt
    12061 atcgggcaac gattaggtga aaagacagcc catgtattgg acacgaaaaa tcgtctagtc
    12121 aataaaagaa agcaggcaaa acaacagata caggcgacac caacccatgt caaatatgcc
    12181 cttcatcaag gaaaagagaa tgtcactcat aatgtaacag actttaaaca atctatggca
    12241 gataccaaag ctcaacgcaa acaacagcgt atgaaccagc aggagcaaaa acgaaagaac
    12301 attgctcaaa agcgtttgga gctggataaa gcaaaagaaa aaagacgaaa agaaaaccca
    12361 tcatctcctg ttcagaataa ccagcagcat gttcgcaagc gtcagcaaac cgtcacgcct
    12421 gttttgtcac aaaacactaa acgtgaaaat catcagcgac cattgaaaaa agagcaaaaa
    12481 caaaagacac caccgaagca attcaatcag aggaagctaa ataaaagaac ggataagcga
    12541 ggacaacaaa catgaagcta aaaataacag tgatttgtag tgtggtcttc tctctcttct
    12601 tttttctctt attattccta gtcattttct ttgcagatga tacggacagt ggcgaaaaca
    12661 acaaggattc ttctattcca caaggcggtg tgaccgtttc accagaagtg ttggcacacc
    12721 gtccgttgat tgaaaaatat ggtaaggaat atgggattga agactatgtt tcttatatct
    12781 tagccattat gcaagtggaa tcgggcggta cagcagaaga tgttatgcaa agttccgaat
    12841 ctttgggttt accgcccaac agtttgagta ccgaagagtc catcaaacaa ggggttaaat
    12901 atttcagtga attattaacc agtgcagaac aacaaggagt ggatatagat tctgttatcc
    12961 aatcttataa ttatggcggt ggctttttaa attatgtgag gagtcatggg aaaaaatata
    13021 cctatgaatt agccgaacag ttttctaaag agaagtcggg tggtcaaaaa gcagactatc
    13081 ccaatcctat agccatacct gtgaatggtg gttggcgata taactatgga aatcagtttt
    13141 atgttcagtt ggtttcacaa tacctaaccg acacttcccc aacagaattt gatgatgaaa
    13201 ccgtgcaagt cattatggac gaagccttga aatacgaagg gtttccctat gtctttggag
    13261 gggcgtcgcc taccacctct tttgattgta gtggcttaat acaatgggtg tatgacaaag
    13321 ctggcatttc tctccctcgt gtcgcccaag atcagtatga cgctacgcaa gaaatctcta
    13381 tggaagaagc ccaagcaggc gaccttatct tctttcattc aacctataat gcaggcacat
    13441 atgtgactca tgtggcgata tatttagagg gaaatcgctt ttatcatgca ggcgacccaa
    13501 tcggttatgg cgacttaagc agtcgttatt ggcaagacca tctgattggg gctagacgtg
    13561 ttattcataa ctaagaaagg aatgaatcaa atgattataa aaatcgagcg aaaagaaaag
    13621 aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa gaaaagttcc gatgattaaa
    13681 gttggcacgc ataagaaatt aacctttgtt ttatgggttt tattgattgg tagtgtcgga
    13741 tttggaatct ataaaaattt tactgccatt gacactcata ctgtccgtga aacagaaatc
    13801 attaaacagc agattgtcga taccaaccaa gtagaaagtt ttgtgaaatc ctttgcgaaa
    13861 gactactttt cgtggcaaca atcccaagaa gccattgata aacgaaatga aaaattgacc
    13921 cattatttga cggaagaatt gcaggtatta aatgaagaaa tgattcgtaa ggatattcct
    13981 actagttcta gtgtgaacga tatacaggtg tggcaggttt ctcaagtgaa tgagaacacc
    14041 tttgaggtac tgttttctgt tgaacaagtt attaccgaag acaaagacaa ggaaactata
    14101 tcgtctagct ttcatgtggt tgtccatata gatgaatcag ataatatggt tattatcaaa
    14161 aatccgacaa tgagtaagaa acctcaaaaa tcggactacc aacccaaaca gctagaaagt
    14221 gaccatacag tagatacaga aacgatggac gaaatcacta gctttttaga aacgttcttc
    14281 cagctctatc ctaccgccac agaaaaagaa ttgacttact atgtaagtaa tcatgtgtta
    14341 cctatgatta acaaggaata tgttttcgag gaattggtga atcctatctt taccagaaaa
    14401 gataaccaag taatcgtgaa tgtcgctgtg aaatatttag atcaagaaac aaaggcaacc
    14461 caaatctcgc aatttgagtt gatattagaa aaacaagata attggaagat tgtaaaataa
    14521 caaatattgg tacatgatta cagatacttt gtaatcatgt actctttttg ataaaaaatt
    14581 ggagattcct ttacaaatat gctcttacgt gctattattt aagtgactat ttaaaaggag
    14641 ttaataaata tgcggcaagg tattcttaaa taaactgtca atttgatagc gggaacaaat
    14701 aattagatgt ccttttttag gagggcttag ttttttgtac ccagtttaag aataccttta
    14761 tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt tgtat
//
LOCUS       DACDXE010000073        14779 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.073, whole genome shotgun sequence.
ACCESSION   DACDXE010000073 DACDXE010000000
VERSION     DACDXE010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.073"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(147..773)
                     /locus_tag="IWM23_002277"
     CDS             complement(147..773)
                     /locus_tag="IWM23_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884886.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(1068..1403)
                     /locus_tag="IWM23_002278"
     CDS             complement(1068..1403)
                     /locus_tag="IWM23_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAP9884887.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(1390..1674)
                     /locus_tag="IWM23_002279"
     CDS             complement(1390..1674)
                     /locus_tag="IWM23_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAP9884888.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(1730..3253)
                     /locus_tag="IWM23_002280"
     CDS             complement(1730..3253)
                     /locus_tag="IWM23_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAP9884889.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(3246..4421)
                     /locus_tag="IWM23_002281"
     CDS             complement(3246..4421)
                     /locus_tag="IWM23_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAP9884890.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(4425..4610)
                     /locus_tag="IWM23_002282"
     CDS             complement(4425..4610)
                     /locus_tag="IWM23_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884891.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(4576..6270)
                     /locus_tag="IWM23_002283"
     CDS             complement(4576..6270)
                     /locus_tag="IWM23_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAP9884892.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(6267..6740)
                     /locus_tag="IWM23_002284"
     CDS             complement(6267..6740)
                     /locus_tag="IWM23_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAP9884893.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(6809..6964)
                     /locus_tag="IWM23_002285"
     CDS             complement(6809..6964)
                     /locus_tag="IWM23_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAP9884894.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(7098..7478)
                     /locus_tag="IWM23_002286"
     CDS             complement(7098..7478)
                     /locus_tag="IWM23_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAP9884895.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(7482..7697)
                     /locus_tag="IWM23_002287"
     CDS             complement(7482..7697)
                     /locus_tag="IWM23_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884896.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(7700..8107)
                     /locus_tag="IWM23_002288"
     CDS             complement(7700..8107)
                     /locus_tag="IWM23_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAP9884897.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(8373..9848)
                     /locus_tag="IWM23_002289"
     CDS             complement(8373..9848)
                     /locus_tag="IWM23_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAP9884898.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(9838..10686)
                     /locus_tag="IWM23_002290"
     CDS             complement(9838..10686)
                     /locus_tag="IWM23_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAP9884899.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(10723..11085)
                     /locus_tag="IWM23_002291"
     CDS             complement(10723..11085)
                     /locus_tag="IWM23_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884900.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(11385..12009)
                     /locus_tag="IWM23_002292"
                     /pseudo
     CDS             complement(11385..12009)
                     /locus_tag="IWM23_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(12006..12317)
                     /locus_tag="IWM23_002293"
     CDS             complement(12006..12317)
                     /locus_tag="IWM23_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884901.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(12361..12654)
                     /locus_tag="IWM23_002294"
     CDS             complement(12361..12654)
                     /locus_tag="IWM23_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884902.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            12848..13495
                     /locus_tag="IWM23_002295"
     CDS             12848..13495
                     /locus_tag="IWM23_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884903.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            13556..14701
                     /locus_tag="IWM23_002296"
     CDS             13556..14701
                     /locus_tag="IWM23_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884904.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
BASE COUNT         4266 a         3045 c         2347 g         5121 t
ORIGIN      
        1 gaaacccata tcaaagaact ataaaggtta tcaaaacagc gtttatgact aaatgaatta
       61 gagttatcta tcactatttt tcaagcgatt taaaccaaat ggcatacaat atggcataca
      121 aaaacttcaa taagccgtgg gggtttttaa atttcttctt gttctttaaa atcaatgagt
      181 ggagctacat aatttgaatc cactttttca aacgtcactt tttgcatcga cgaatgtttt
      241 ctttcccaag ataacgtaaa tagatttgac tctggactta taaagtctac ttcaccttct
      301 agtattactt tcttatgcaa atattcctct aataaatata gacttgctaa agcatacaac
      361 acattttcca aatttgcttc cttataattt gaagaacgac catgttttac tctgttatat
      421 attttccacc aatctggtgt atcttcaggc cacccatctc caaaaggttt tagtgaagtc
      481 aatgttaaag aattcataac cctaacacca ttatccaaac acattaattc gctagattta
      541 ataacagctt taaaatcagt tatgctttta ttgctactgc tatcaaatcc gcaaagttct
      601 ttgaaaataa tctcacactc cgaaccaatc gctagtagta gtttttgata ttcaatagag
      661 aatgttttga aattattctc atcgagcatc acaaaatctg tggtattaat aaactcattt
      721 tctaaatgta gataatactt ccaataaaaa tttgtaaact ctgctttgtc cattaaatca
      781 cctcttttga attataacaa aaaaaacacg tccacaacag acgtgttctt tgatttctac
      841 acaatcagct tgccaaaacc atccaatcag tcctagcatg cctgtattaa aattgaacgt
      901 tcatcatcta tcatctttct tagtccagca tgtttgcaac cacacctaca attttcagtc
      961 caacaataga tttcgactag ctaagtcttg caaaattcag gcagctaacc cttcatttac
     1021 taccttaatt atactaaata cccacaacgt ttacaagtgt aatcattcta agaaatctgt
     1081 ttagctatga tcgttgtata atctttaaac gccgttcctt cattgattga aataatttta
     1141 taactcttgt ttttccaagc tactttcata tcgtttgtta gttcataatc ttgatcgtat
     1201 cgaataataa acgtcaacgt tccttcaaga atggtaccta cactagcaag cacgtctttt
     1261 agcatttgtg aacgcacatt agcccaacat tcaaatacag tttcttcact atccaccatc
     1321 tgcccatctt catcttttac ccgttttgtc tttttaaact tgactctctc agtcaattca
     1381 tttacattct taatgattgg cataataagc acctctcatt tgttgtacta atgcttgtac
     1441 cgttactggt atccgttcgc tgctggcttc ttgacggttc aaataccaat gttgagtaag
     1501 taatgacacc gccaaatcaa attgctgata attatccata tctttatccg ttgcgctact
     1561 atcaatagca ctttttatat attcgcctgc tgtatcaatc aattgttgaa taaagtcatc
     1621 atcaatagtg tgatcgatcc gcatactttt ttttatgcta tccaaaatta ccattttatc
     1681 tctcctaatg aaaaaaaggc agtgggataa accgccgccc aatatttttc tatacttttg
     1741 gtgttacaac tggcgtaaac tcaatataca ctgaagcatt ttgatcaatt ttcttataat
     1801 cgttgcgaac gatcactgca agtccttgtg agtagtaatc gaatttttcc cattgagtcg
     1861 ttacttgatt tctacgagca acaaagatag attgtgcaat atcccccata atcataggga
     1921 atgttccagc tttaggattg gcaaacaatg tatctgcaat caatactact ggcataccga
     1981 ataatgattt accactaggt gctgtcacat cgggttgtaa aatgtaacgt ccatctgaat
     2041 ctttcaatgt atctaggtgg taatatccgc tttggtttag aattaccatt ttatttaatg
     2101 ctgggtctaa tgccacattg tatagttgtt tcaaatcgtc caacgtagca gccgttttct
     2161 tagtgaatgt ttttaataaa tccatgatat gcttattgtc agtgttttct accaatttcg
     2221 ctaactgatc tttgacctct tgtacaatat tcactgctga atcttccaca acctcatttg
     2281 ataaggcaat cttaccagca cgagtttcta ctttataatc aactgaagta aacatttcag
     2341 cgtcaatatc accaatttca gcaagttcag cttttgttgc taacactgct tgttggttag
     2401 ttgctactgg atatttacct tgtccatttg atactgtttt tactgttgca tattgagcta
     2461 agttataatt tgaacgtttc aaatcaaaga cttcaccgat tacttcttcg ggaacaactg
     2521 ccgctgcatt tacagtagtg actccatcac gcacttcgcc ttgtgaacgg atatattctt
     2581 catacccacg caattctgtt ttttcgttat cgattaaagt tttttccatg ttattactct
     2641 ccttttgtgc tttagaagtg gattctaagc tattgtttgt ttgttttgct tggttgttac
     2701 tcataaacga ttcgtaagaa cgcttattta cttggacatt tgatgaatca taagcgggaa
     2761 cggtcacgac actaatttca ttaagtgctt tgattttatc tattgatcgt tcaattgtgc
     2821 catcttcttt tttgtcgaat gaatctaatc caagcacgaa cccaaaggac atagaatcaa
     2881 caaccccttt tgagatattc tcatacacat catttgaata ggtcgtgtcg gttagctctg
     2941 cttcaaaatg taatcctgta tcatcgacat tcaatttcaa cgtacctgct ttgacactag
     3001 ctagtggctt tgaataatcg tgtccatata ataagaacac atttgataaa tccacctctt
     3061 ttaatgcttc gggtgtaatc acttctacaa agccgcctaa atcttttgac ggttgcccga
     3121 atttcaacgc atagcctgaa atagttttgc cgtctgttgg ttgtttttca ttttctttct
     3181 ctactttggg agaatcggct gttagctcag cttcttctgt cagccgtttt tccttttctt
     3241 cttcattatt caactggtgt aactccttcc tgttctttta attgatattt ctccaacgta
     3301 tctaaatacg taaagttcaa acttgctagt aaacgatcgc caccatccat ggggggtagc
     3361 cccattttcg accgcccctc attaatcgtg agaagtgacc ctttgacctg atccaataca
     3421 ttttttactt ttgtttcagg atcagtctct agtaactgat cggaattaaa gcggatattg
     3481 gtttcaagtt tcgtatctaa ttcactcaca aacacattaa agtaatgaat cagcgtactt
     3541 tggatatact ggagattact ttgaacggtg ctagaatgtt cattttcaac gcctaaacgc
     3601 tcaatcggta caccaaacgc cttggcaatt tgttttgtgt tccaatcatt ggaattgata
     3661 agcttcaaaa catcggtgtt tatttctaat gttttgtagt ccattgtttc atcaagaatg
     3721 atcgttctaa gagcattatc tccactactt gaaccgttgg cttcttcaaa cttttcacga
     3781 attgcgttct ttgctgatcc gtctaaatct gacttatgaa ccttcaaaat acctgaccca
     3841 ctgacaccac gagtaaagaa attgtgcaat gtgcggttgc cagcttgttg aattttcagt
     3901 tcatcacgta acgcataaag tggtggtaat cctgttaaac catcttgggt gaaatatttg
     3961 aagtgcaaaa tatcgctaga cttcacacgt ctttttttat caccaatctg ataagacaac
     4021 gtaccattat ctaattgagt aaccgttaca cttgagttag gtaataagtg aatttcttct
     4081 accttttcac cgttgcgttt aatctctgca aagctattac cgtttagcaa catattgacc
     4141 gccaaagcaa actttaaatg ccaaccatcc atttctgaat taggtttctc gtttagtaac
     4201 ttcaccagtt cattatcagc ttgtggcata ttgtttttga ccaattgaat cggacttgaa
     4261 gcaatatcac tagcaataat tcgaacggct gcaaacacat cactatttct taatgcacga
     4321 acgcttgtaa agctggtggt gtaatcatcc gattggattg ataccacatg ttgtaggaaa
     4381 ggatctcccg ttgtattttg tctcgcttta aaaaatgcca tcttttaacc tcccttctgt
     4441 tctgtattta gcatgaccgc taccccaata agcactatgc ccagcgctaa caagccatag
     4501 tagacatttg ttagtaaggt gatagcaacc aacacacaca ccaatccaag aagtaaaaga
     4561 acggtatgaa tgtaattaga aagaaaattc ttcacttgta taaaattcat tgtctgcttt
     4621 cgctccttcc gttgtggtga agtaatccat tgcaaacaca taggcattga ttaatgcagc
     4681 tattggatca attttgttac tgttctttga tttattaatc tgaatgccgt tgttatccgt
     4741 ttttaaaata gcgttgttca ctgcatgggt gagaatcgta tttttattgt gaataacgtt
     4801 gccttcataa acttgttctc taaaagttcg ggtcggaacg tttagagtaa tcgttccttg
     4861 tcttacttct aacattgggt agttggcttt ttcagcttta ctgataagcg aattagcgtt
     4921 atacgggtca taacaaatcc ccatacattc caaatcattt tcttgaatca aatcttgaat
     4981 aaactgaaat acttggtcat agtccacgat tccactttct aattgagtaa tggaacactc
     5041 accagctttt tctagttctc gataaggcaa accgtcgcgc ttttctttgt cttgaagccc
     5101 atatttggtg gctacaaagc tatgtgaatc acaataaagt tcgccgttat ctagcggtac
     5161 gatccatgaa acgctagtta aatcatctgt tttagataaa tccactccga tataaacggg
     5221 cttgcctgta atatcttgtg gttctacttc ggttgcctgc cagtcttcag caatcatata
     5281 gctgtcctca ctcgcttgtc tccataaatt gaagtttttc accaatacag aattaaggtt
     5341 attttgtttc aaagcaaggt tcacatcatc ttgaatggtt ggaatcatta cttttttgat
     5401 ttcttcactt tcaaaaatcg gattggcttt gatccatttc tcttgatcgt gaatttcttc
     5461 gggatcgtcc aattcccata tcgctataaa gtacctgtcg gcttgttctt cgccttttag
     5521 cacacgatct aacaataagt attcctcgta cataggaacg tttaaatcaa gcccagcggt
     5581 actaataaca cacaataagc cgttcttttg ttgggtcatt cctgacttaa tgacgttgta
     5641 cgttttacga gtttttgctt cgtgccactc gtcaagaatt gcggttgttg gtgcaaaccc
     5701 atctagcgtg ctggtttcac tagccagtgc catagcgaac gaattagagg gcaagtgctg
     5761 aatcttagag ttcataattt tcagttgtgg tcttaaaaac ttactagact tgaccacgtt
     5821 tctaagtgaa ttagacagca tatcatagcc caatttagct tgtttcaaag cgttacttac
     5881 aaacaatact tgccttgctt ctgctggttc ttgttccata atcaacgcat tggcagccat
     5941 gccacttgcg atatacgttt tcccattctt acggctcata ctggtaaacg ctcgattgaa
     6001 tcgcctgtaa ttcccttctt tagtacgcca gccataaaga ctgcctagaa tgaatttttg
     6061 aaatcctaac atcttgattg ctttaccgtc cgtagatggt agcaactcaa tgaatttaat
     6121 cgctttggtg gcttgttctt catcgaacac ataagggaag ctatctgact tagaacgctc
     6181 caaatcgctt atatgacgct cacacgcttg gataattttc ttgggtgcaa tcagcttacc
     6241 tgataggact ttctcaatgt aatcattcat gaattcatca actctttgaa cgggtcttcg
     6301 ggttcatcat ttttaacctt gttcaaagct agttttgatc gtgattctaa ggtcatacct
     6361 agtgaggtgg cttgtttttt caaatcactc atagcttgtg attgaacccg aaccgctgga
     6421 ttggctttct ttactccgtt ttcgcatgtg ataaacgtac ctgattttct tatctcggct
     6481 tcggctgttt tcattcgagc aaaggctaaa caataggcta ccaatgaacc ataatctgct
     6541 tcactcaatg gataatcttt ttttaatgtt ggtacgatac gattccattc acttctagcg
     6601 ctcgctgcca tccagtcagg gggcgtgata tttattaatg gttgctgctt gaacaattct
     6661 tctctagcgt ccacacggat tgctttttct tcacttgata tattgccttt tgtatcgtgt
     6721 aatattttag ggtgtcccat gttgtcctcc tttcagacaa aagaaaaagg aacgtaagca
     6781 attaagctca cattccttcg tttttcgatc agaatttttc tttatctaat ttatctatat
     6841 aaatgatttt tccgttctgc atttgaatgg tcacttgacc aaaatgaggt aactcaatgg
     6901 tgcgctgttt ccctgtttta tcagtaataa caattgtatt tttcaataaa tctcctactt
     6961 tcattatata ctttttcact tgtgattaca agtgtaatcg ttatttgtaa ttacccttat
     7021 gccttttcac aacggttatt tgtaccaaaa tgagtaggca tcgattgtca gtaagtttca
     7081 cactatgccc ccttaactca aagggtactc ttttctcgtc tttgtctttc attggctgtc
     7141 ttggaattat ggtgtgcatg gcataaagaa cgaaaattag tttcatctaa ccgtctattc
     7201 caatcatctt ttagttcaat tttgtgatct acaacatcag ctttccttat caagccttct
     7261 tctaggcaat cctcacatac tggatgattt aaccgataca agtatgaagc ctttcgccat
     7321 gttctacttt gatagaattg aaaatattta ccttcgcttt gctttctttc ttcacgctta
     7381 attgccgttt gctttgcttt atgcttccca caatacttct gcctataatc aatcaatgtt
     7441 ttacatccag catgattaca ttgtttttta actgccatag attaccaacc aaagcgatct
     7501 cgttcgtcag aatcatcttg ttcccatttt gatttgtatt caatataagg cgaaactatc
     7561 ttcaagcacg ttccgtcttt aaaattaact ttagtgattt tatcttcctc aaaactaata
     7621 aagtctacat cttcttgagt gtttgaatac tcaatgtgtt tctcaccatc gaagtatcta
     7681 atactttcca tatgcataat tattctacct ccaataaatt agttatcaat tcatcatctg
     7741 ttagtagcaa agccaagaac ttatcatgga tgtttaaaaa caaattatcc tcattcgatg
     7801 taagctctcg ttgtctcgct cgatcatata ggttaagcag acaattaaat tcatccatta
     7861 gctcacctgt atcatcatag ttgccactat ctaccaatga tcccactact agcgattgac
     7921 accacgctac tgactcctgc agccaatcag gttcttcttc cattatcttt aatacttctg
     7981 gatcaaatgt ggaataatct tctgttacca atcccacact aaatgccagc gtgttcatta
     8041 gaaaccgatc catttcatct gtatattgaa agttcggtgc tttgtttgtt gaaataatat
     8101 tgagcattta ctttcctcct tgattcgtaa cacgtggtaa cacctacgta acaggtaccc
     8161 gttaccactc aatcccaacg gggacaaggg ttctagcgtt ttggtaacag gtaacgggta
     8221 aaatccagta acattattcc ccagcatatt agctaactat ttcaaaagtt ttaaggaaat
     8281 aaggtgttac cccgttacca aattgctgta tcccttgggg cagtaagggt aaactagtaa
     8341 cgggctaggt gttactgacc cgttactacc cgttactttt tgtactttcc agctcgattt
     8401 ttattttcat aggcatttga cttattgtaa aatcctcgtc gtttatttct ttttggcagt
     8461 tgcttccgtt cccaaccagg aatactgcta ataatcaagg caatcttttt agtttctgca
     8521 tctgatcgac cattcaataa atcctttgct tgtttatcaa aaacagcttc caaaatatca
     8581 gcggtgagta cgctatcaac taaataaaac tcttgttgca tagtagctcc attatagaaa
     8641 tgctgtaaat ttttctcatt acatttactg tcaggatagt tctgataata agaacgtctc
     8701 gcccacgctg gagcttcttc ccatttatac gggatttcca tttccaaata tttagtgatt
     8761 gcttctttaa ttggattctc catcttggca tcctcttgat agtcttttgc aatttctaag
     8821 acttctttat cttcatcggg actaaaatag attcgttgtc ctttatcaaa aagcaccttt
     8881 gcttcagcta acacttgaag aaaatattca tcctttgttt taaacggatt catctttggc
     8941 ttattcttac aaggcaaagg ataaaaacgg cggtttcctg tatcatcttt caaatactgc
     9001 ccatcattcg acgttccaat aaatacacat tgccttgccc aagctatgac attccgacca
     9061 taaggaggtc taattttatc gatcttggca ctgatgaagt tttttatctg ttcaatttta
     9121 gttttgttca ttgaagataa ttctccaagt tcaataatta cattgccttg aagctgcata
     9181 taatcatctt tcttttcacc tagcgaatct aatgaatcgg tgaaaaaatc atccgtgtaa
     9241 agtaatgctg gaacggtact cttaccaatc ccttgcggtc catccaaaac tggaacgatc
     9301 tcaaacttta ttcctggttt atagatacgt gcgactgatc cgactatcca acgttttgtt
     9361 acttctcgtg tgtaaaggtt gtcgtctacc cctaaaaggt caataaacag tgtttccagt
     9421 ctaggctgtt gatcccattt aactttttcg atacgttctt ttactgggtt gaaaacattc
     9481 tttctagcca catttactac tgctttgtgt attttgttgt catcaaaaac tacattgtag
     9541 ttttcttcaa tatatgccgt taaggcatcc tcaaaactgt cttcccattc tccagcggta
     9601 cgattgatcc aaggagaatc ttttaatagg tgaatgtatc cgctaaattc gttataagca
     9661 attgttccag ctaaattgct atcattggtt aaaatcaatt ctgcattcac aacactgtta
     9721 cttttaatct gatcgtcttt attgcatctt aatcgtgaca tccaggtttt caattcaatg
     9781 atattgtctt cacgctcgct ggcttcggga aagtctaaaa tactatctaa tttatgctta
     9841 tgagccatga gctttccccc tcaaatcttt tttaatcatc gatcgaataa tttgattcac
     9901 ttctttatct tttagcggtg gttcattgta cgcatttgct gtataaatca attctgctaa
     9961 agtttgatta tctacaccgt gccatagtaa ccaaccgcat aacttagtta aatgatcgtt
    10021 tctactacct tcgccagcgc cttgaacaat catatctaat ttttttgctg tccctgtttt
    10081 tgctacgggt cggtattgag tctttatttc ttgcggtttt tttagcagta ggttcactag
    10141 ccaatcggga gctggtgcaa tattatttag agatacgttt tcaataggtt catatgctct
    10201 cccaacactt cttgaaataa tcatagttgg cgctattgga actcctttag tgataatatc
    10261 aatcccgctt aaacttttac ctttatcatc acgcccgaag atatcagcaa atgcacctgt
    10321 ttcttgttgg ataattacac catcaggaat tttaaagaag aaatggattc ctttacctgg
    10381 tgtgatttcc gtataagtag aaggaaatgg atcatacatt gaagcaagtt ttttataatt
    10441 ctcaacgccg tttcgtgttt cagaatgttg atctaggtca actaccacca tttgatgatc
    10501 ttcaagcatt aacccaatat ttctagttgc atcttccgtc caccattcat taatttgatt
    10561 agaatcgtta gttgcatcat tccaaccact agagcctttt attggaattt tagttccagg
    10621 agataaggga taaattgacc atcccgactt ttgtaattcc gttgctgctt gataattatt
    10681 tgccaataaa cctctcctcc tttttcaaaa tactggtata aatcattcag acacctccat
    10741 gagcttcaac tgctcaagtt cgtatttcaa cccatcccga acaaattcag caccttttaa
    10801 ttgattctga tactttaccc atgtattaaa tgttttcacc cttgcttctt gcatatcagt
    10861 aatgtttaac catgcggtta aaatctgatt ttttagctcg ttgatttctc gacaaattat
    10921 tttgtgttct gcatgttttt tgagtacata caaaactgtt agtttttcat tgaatgaatt
    10981 tccaaagtag gttttcccga tattattttc tagttcagcg atcccttgat taatttcctc
    11041 taattcaagg ctatcgagtt ggtttaatag ttttgttttg tacatctact tatgccctcc
    11101 agttcttaat tgatagtcgt actcgtcata agcagtgaag agatataacg ctaatcctgc
    11161 aaagatagct atttttgcaa ttaatggtaa taagccgaaa attccgccga ttaaaatgct
    11221 tcctataatt aagtttcgtt tcataatggt tccctccgat tttttaatat ggtataatcg
    11281 gaacaaagaa tatcgggttt tatcccaatt aagttgcctt tatcctgtcc gccaagactg
    11341 ttatggataa ggctttttct ttgttctcga tcatgttaat gctcctaagt tgtctgcaat
    11401 tcagcagaac gattataaat tttctcactc actgaaaact gcaacccata tttttcttta
    11461 acagtcatta attctactgt ctcatctaaa ataggttcac gatcaaccaa catttcagga
    11521 gtcatatctt tcttttttac cattttggaa tgaccataaa tagttgatac agctttattg
    11581 gctatcacat tcgctttaat gtagtctact ggttttggtt cagtaatagc gtggcttaat
    11641 ttagtcattg cttctttttg gtgttctttg tctagcatgc ggaatacttg aaagccttca
    11701 agtcctgtgg actggcgtag ttctttgatg atgtcgaaaa tccagtcttg aaatgcctcg
    11761 gcttcttttt tacggctttt gaatgccatg cggtaaatat tttttttcgt tgatgatgtt
    11821 tacatcttgt gttctgccca atgaatcgat gaccttacta gtagtaaccc catctttagg
    11881 caaagagtga atggctctag taacctgttt aagacctaac gcttccgcaa catctttcgc
    11941 cactgcccac cattcatcat taatatcgac aaatcttata atgtgtccgt tccaaatttc
    12001 agttttcatt caacgtcctc cagttcaagc cctagcaagc tcgctaacaa ctcaacttgc
    12061 tgatacaaaa tttcttgata ctcttcaata gaaagtcgtt ctttaaactc tgcttgtact
    12121 aaatcgttca agccacgcaa tactccgctt accgttggtt cttgtggtag caatttttga
    12181 tagtccattt gttcgtacaa acattcaaac gtttcttcta gcgctttgcc ttccaagttt
    12241 gctaattctg ccgctgtgtc ttctgtaaga attgttgata atactttttc gattgcttta
    12301 ttttgtgtcg attccattat tatttcctcc tattaatccc tttgccccca cggcttgcgt
    12361 ttatttttgg tattcttcca taaagcgatc acattcatct tgtgaaattc ttacgactcc
    12421 atcaactata atcaacttta aaccgttagg aacatatttc tgcatcatag tattgtatga
    12481 aacgttcata tacttagcag cttgtgcctt gttcatatat tttggaaact ggtacttttt
    12541 ttctaataat gaaagtaacg ttccttgagt ttgctctact tgttgaacaa ctcgatcgtc
    12601 aataatttta aaagctggat catcaagcgt gagtttgtca atttttaatt ccatatttat
    12661 cccccctcgt tagcatacat ttctatacaa agattttaaa aaaatgcaag aggctcttga
    12721 atacatttct atacctctta taaactcaat ataccatcat accttttcat ttgcaagaat
    12781 tttaacatac ttttttgaac ttttatgttt cacacatatt taaatcgtga tatagtagag
    12841 aaaatatatg aatggtggtg aaaatgtggc tagaaaaaaa taccccgaag cagtggagac
    12901 aggacaaaga attagactgg ctagaattga aaagggactc tcaatggaac aacttggagg
    12961 taaattatcc ccaccggcca gtaaaggtgc cgttagtaat tgggaaaatg gctataatct
    13021 tcccaataac accaggctta aacaactttc aaaggtatta gatgtatcca ctacctatct
    13081 attaaacgga tcttatacat taagagatat gcatatgatg cccgaaatta agcgaaatgt
    13141 gcttgaaaag caattgaaaa aaactattga aaaaaataac tctataattg attctaatct
    13201 ttctaatgct atcaataaca tcgaagaaga taacctgact gtctcacaga agatttattt
    13261 aacaaacgca ataaaatatc ttaaaacagg atctcgaatt gaatttcctg acgggagtac
    13321 cccaattgct tatctcgcag ctcttctaaa tacgctttta cgatatgact cattaattaa
    13381 tgattcagaa aaaaatacag atgagaaaaa agagtatcaa gaattttatg aaaatgatct
    13441 tctaaaagag ttcacagctc ttgttactga gctaaataaa cactattcaa aataataata
    13501 aactgcctcc tccaaccgcc cccacggctt gctacactat ggagggaaaa ctattatggc
    13561 aacattcaag caatatgaaa ctaaaaaagg taaattttgg ctctatgaag cctatcttgg
    13621 aatgaacaaa atgactggca aacctgataa ggttcgtaga cgaggttaca aaacaaaaaa
    13681 agaagcccag cttgccttgt cacgcttgca agtggactac gataaaaacg gtattaaaaa
    13741 agcgaacaac gaaacgttta aagaagttta tgacttgtgg ctagaaacat ataaaaccac
    13801 tgtccgagaa gccaccttca tgaaaactga aatcaagttt agaaagtgga ttcttccaaa
    13861 atatggaaat cttcgtgtga atgaagtcac ggtaaaaaaa gcccaacaaa ttgtaaatag
    13921 ctgggctgaa actactgacc aatacaaggt acttcattca acttctaagc gtatttttag
    13981 ctacgctatc aatcttggga ttattgagac taatccacta gacaacgtat taatgcctaa
    14041 aagaaccacc actaagcata aagaaaaggt caaagtttac agtaaagaac aactgatcac
    14101 cttgtttgat tacctaaaca gcaaacctga aacttatcga aacgactttg ataaaacgtt
    14161 actacgcttt ctcttttata gcggggttag gatcagtgaa gcccttgcat tgaattggtc
    14221 agacattgat tttcaagaaa atacggtcac catcaataaa acactaagcc aatccaaaaa
    14281 tggctataaa atcagtgatc ctaaaactga ttgtagtgct ggggttcttc cactagatga
    14341 aagaacaatt tctatattaa aaaaatggca gattaatcag cggaaatata tgctgacact
    14401 tggtgtaact gatccgacta tgattttttg tggtatctat aaagaactaa tcacacacca
    14461 cgctatttat gcaagattga ttactatttg tgaaaaagct atggttccat ttctcggtgt
    14521 gcatgtagca agacacactc atgcttcaat gctcttggac gctggagcta ctatggtaga
    14581 agttcaaact agattacggc atacaaaaat tagtcagacg atcgattgtt acggtcactt
    14641 ggcaaaagaa acgaaagaaa agactgttga aaaattagta caacacttaa atattgacta
    14701 gatggcatac aatatggcat acagtaaaaa ataaaaaaag gagaaaccct atcatatcaa
    14761 ggtttctcct agctttata
//
LOCUS       DACDXE010000074        14569 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.074, whole genome shotgun sequence.
ACCESSION   DACDXE010000074 DACDXE010000000
VERSION     DACDXE010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.074"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(374..736)
                     /locus_tag="IWM23_002297"
     CDS             complement(374..736)
                     /locus_tag="IWM23_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884905.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(810..1190)
                     /locus_tag="IWM23_002298"
     CDS             complement(810..1190)
                     /locus_tag="IWM23_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAP9884906.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1272..2447
                     /locus_tag="IWM23_002299"
     CDS             1272..2447
                     /locus_tag="IWM23_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9884907.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2524..3108)
                     /locus_tag="IWM23_002300"
     CDS             complement(2524..3108)
                     /locus_tag="IWM23_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9884908.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3309..4307
                     /locus_tag="IWM23_002301"
     CDS             3309..4307
                     /locus_tag="IWM23_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9884909.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4419..4928
                     /locus_tag="IWM23_002302"
     CDS             4419..4928
                     /locus_tag="IWM23_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAP9884910.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4958..5617)
                     /locus_tag="IWM23_002303"
     CDS             complement(4958..5617)
                     /locus_tag="IWM23_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAP9884911.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5619..6383)
                     /locus_tag="IWM23_002304"
     CDS             complement(5619..6383)
                     /locus_tag="IWM23_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAP9884912.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6391..7068)
                     /locus_tag="IWM23_002305"
     CDS             complement(6391..7068)
                     /locus_tag="IWM23_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAP9884913.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7105..8490)
                     /locus_tag="IWM23_002306"
     CDS             complement(7105..8490)
                     /locus_tag="IWM23_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAP9884914.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8700..8784)
                     /gene="ffs"
                     /locus_tag="IWM23_002307"
     ncRNA           complement(8700..8784)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IWM23_002307"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8807..9322)
                     /gene="tadA"
                     /locus_tag="IWM23_002308"
     CDS             complement(8807..9322)
                     /gene="tadA"
                     /locus_tag="IWM23_002308"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAP9884915.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9429..10286
                     /locus_tag="IWM23_002309"
     CDS             9429..10286
                     /locus_tag="IWM23_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAP9884916.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10367..10702)
                     /locus_tag="IWM23_002310"
     CDS             complement(10367..10702)
                     /locus_tag="IWM23_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884917.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10769..12139)
                     /locus_tag="IWM23_002311"
     CDS             complement(10769..12139)
                     /locus_tag="IWM23_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAP9884918.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12217..12753)
                     /locus_tag="IWM23_002312"
     CDS             complement(12217..12753)
                     /locus_tag="IWM23_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9884919.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13062..14543
                     /locus_tag="IWM23_002313"
     CDS             13062..14543
                     /locus_tag="IWM23_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9884920.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         4485 a         2898 c         2366 g         4820 t
ORIGIN      
        1 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
       61 atgtcgttag taacatgtca ataactattt tttaaatttt ttagaaatgt gatataacat
      121 ccacttcaga atcaacgctg ttaaatttat catgtatttc ttccttggtc aactataaat
      181 taagcattac tttgtgaaat gatttttcga tcaattatta ttattcgtta tacaaccgtt
      241 ttcaacatat tttgaaaata aaatgcacta aaaaactatc tttagcagaa catttcattg
      301 tattttttgg accaaacaat aaaaaaagaa gtttgaaaaa aattcaaact tctttgttcg
      361 cttaatctgt attttagaag aaaggcactc cctctacttc gtaagttcca agaacagagc
      421 gcatttgacc tggttcttcc caaatatatc cttcgcctaa ttcttctctt tgattctttt
      481 gttcagtttc aaagacaaac aacggaacat tttcctcttt aatatgtgta tttgtcaatt
      541 ccatcaaact aatcttgcta acatcgatat gaactatttc ttttaagaaa ttaagtatat
      601 tcgctaaacc agtctttcct tcagaaagtt ccgctaacgc aaattccaac ttgtcatcaa
      661 ttgaatggac caaaaatttt ttagtaccat cctccaaatg caacataatt gtaccagcaa
      721 ctcttttgct tgccaaaatt tccacctcga tcaacttgtt ctatggctta cataaaaatt
      781 ctagcacatt ttcagaaaaa atacactttt tatttcttat tttctttccg ttctagttca
      841 gataatgctt cttctatcca aataggtaac tgctccgcaa tagtggtaaa tccgatctta
      901 cgatttttat tcgtgaaata cctttttctg cgatatggaa cagctggatt tgttttttct
      961 aacgttctta aagataaact gatttttttg gaatattcat caatgtcgat gatctgaact
     1021 gttactggct gtccgacttt taacaatgaa tggatatttt tcgtataacc ggattgtact
     1081 tctgaaacat gaataagtcc ttgcgtctcg ttatcaagag aaacgaatgc gccataaggc
     1141 tgtatccctg tcacttttcc ttcaattatt tgtccaattt tataagtcat ctacttccct
     1201 ctcttcgcat ttatcattat actgtaaata gctcaaaaaa agaagcgacg aaaatgactg
     1261 gaggtcttaa gatgatcgaa tttgataaac agatttcaag aaaagataca gaaagtgtaa
     1321 aatgggacgc aattgctgag acatatcaaa tggatgactt acttcctttg tgggtagcag
     1381 atatggactt cttgtctccc ggtggggtag ctaaagcgtt tagtgaatac atcaaacatg
     1441 gaatctttgg ttatgccact ctgtctgata aactatatca ggctatcatt cattgggaac
     1501 gccaacacca tgctgtggag ttgacgaaag aaaatatcgt ttttacaagt ggtgtgttaa
     1561 ccagtttagc tgcagcagta caaactttta caaaaccagg cgattctatt ttgattcatg
     1621 acccagttta tcctccattt tcctctatta tagaaacaaa tcaacgtcac atcgttcgaa
     1681 gctctttgct agaaaaaaat aatcatttca tcatggactt gtctgacatg gagaaaaaaa
     1741 tcgaagaaaa caatgtaaaa gcaatgattt tatgcaatcc tcacaatcct ggagggcgtg
     1801 tttggagcaa agaagaattg aaacagttaa gcgaattgtg tttaaaacat caagtgattc
     1861 tgtttagtga cgagatccat caagatttaa ctttattcga tcatacattt acttccatgc
     1921 taacgattga tcctaattta gatgatctac tagttgcttt tacttccgca accaagactt
     1981 tcaatctagc tgcaatcaaa aattcaatgg tatttatcaa aaatccagag atgaaagctg
     2041 attttgaaaa acatttagtg atgaaccaac agcacgaaat caacactttt ggactgattg
     2101 gaacccaagc ggcttatgag acgggtggag aatggttaag ccaactgatt ccttatctag
     2161 aaaaaaatgt tcaaacagtt aatcagtttt ttgaacagta tcttccgaaa gtgagaataa
     2221 tgaaccctga aggaacttac ttaatgtggt tagattttac cgcctatatt tcagacgacc
     2281 aagaattgga gaacactctt gttcggaaag gaaaagtcgt attaaatcct ggcatcacgt
     2341 ttggtccttc cggtcatggg catatgcgct tgaatattgc ttgtccagaa gaaactttac
     2401 tggaaggact taatcgaatc aagtatgctt tagagacaat cgattaaatc atttattcaa
     2461 aataaaagga cgagacctta ctaggtttcg tcctttttca taattactcc gtaccatacc
     2521 aaactattct gaaatttcaa ttgtttcaat cactacatca tgtacaggtc ggtcttgtgg
     2581 accacgttgt acatttgcga tttcatctac tgtgtccatc ccttctagta catgtccaaa
     2641 gactgtatgt cggaaatcta gccaaggagt tccgccacct ttatacgctt caatgatttc
     2701 tgatgggaat ccagcacctt ctagttgtcc aagcatatta gctggcacat ttgtgttatt
     2761 tacaataaag aattggcttc cgtttgtgtt tggacctgaa tttgccatag ataaagctcc
     2821 gcgaaggttg aacagttcac gactgaattc atcttcaaaa ctttcgccat aaatactttc
     2881 gccacccatt cctgttccag ttggatcgcc gccttggatc atgaaatcag gaataacgcg
     2941 gtgaaaaatc acgccatcat aataaccttt tttggaaagt tcaatgaaat tttgaaccgt
     3001 cttcggtgct agttcaggga ataattgaac agtaatgtca ccacggtttg ttttgataac
     3061 agcttttggt cctttttcac tttttaaatc tagttgtggg aatgccatat tgtttcctcc
     3121 taatatttgc atacttcata ataataataa cataatacct aacctctcgt aaaaatacta
     3181 gatttgtttt cttttatttt tcatcactag atacattcta taacttaccg tttcgatttc
     3241 gaattcaaac atttttgaga agcattctca attaaattct gctatagtgt gaaaagggag
     3301 gaatttttat ggaaatctat gacattacaa tcatcggtgc aggtcctgta gggatgtttg
     3361 ccgcttttta cgcaggaatg cgccaagcta aaacaaaaat cattgacagt ctacctcaac
     3421 ttggtggtca attagcaact ttgtatcccg aaaaatatat ttacgatatt cctgggtatc
     3481 cagcaataaa agcaagtgaa ttaatcgatc aattagaaaa acaattaact acttttaacc
     3541 atacattcca tttaaaagaa gaagttctct cgcttactcg tgaagacgaa gttatcgaga
     3601 ttactacaaa taaaggcatc cattattcca aagcagtcat tttagcttta ggaaatggtt
     3661 cattccagcc aagaaaattg aatttagata atgctgaatc atttgaaaac cacggattgg
     3721 attatttcgt gaatgatttg atgaagtatg ctggaaaaaa agtcgctatt gctggaggtg
     3781 gtgattctgc tatcgattgg acgttgatgt tagagcctat tgcaagcgaa gtttacttga
     3841 ttcatcgtcg cccggaattt cgtgcgcacg aacatagtgt atcacgcttg aaatcttcat
     3901 ctgtcaactt gcttactccc tatctcatcg atggattatc aggcaataat ggggaactga
     3961 cagatatccg gttaaaaaaa gtgaaaagtg acgagacgat tgatttgatg attgattctc
     4021 tgatcgtcaa ttatggattc tcttctaatt tagaacatct ttcttcttgg ggacttgata
     4081 gtaccagaaa tgccattact gtaaaatcag atatgtctac ttctattcca ggcgtttatg
     4141 cagctggtga catctgtacc tatgaaggaa aagtgaaact gattgctact gggctcggtg
     4201 aagcacctac tgcagtgaac aacgctttac actatattaa tccaaaggaa cgaactcagc
     4261 ctggtcacag cacaagctta tacgataaaa atcttcgtcc tagttgattt tttatatctc
     4321 tttgcatttc aagttaaacc cgaaagcagt ttcttgcatt tttttcatgt tccgttaaca
     4381 tgaaatagag aaagaaaaaa taaaggggga gcaataatat ggtcgtcaaa actgagactt
     4441 tagaagaaaa ggcaagagaa ctattgaagg aacgtggcgt aactgtagaa gatatcgcac
     4501 aacttgtcat gtttttacaa aaagactata tttctgattt gactttggcg gattgtactg
     4561 aaagtgtcaa cagcgtgcta acaaagcgtg aagtacataa cgccattatc actggaatcc
     4621 aattagatat attagcagaa aagaaaaaat tgatgagtcc gttgcaacat attatcgaag
     4681 atgacgaagg cctttacggt atcgatgaga tcctagcctt gtcaatcgtg aatgtttacg
     4741 gtacgatcgg ctttacgaat tatggttata tcgacaaggt gaaacctggt attttaaaag
     4801 aactaaacag ccacgatggc gttcatgtcc ataccttctt agatgatatc gtcggagcga
     4861 tcgccgctgc tgctgccagt cgattagcac acaaagaacc agaaaaaaga agtcgcctta
     4921 ctcaataatt caacaaaaaa agaaagaaaa cgtctattta gacgagttct ttcttttttg
     4981 tttgtttcaa gctatttttt cctaagaaaa gaaaaacagc aaaaaataat tctaataata
     5041 caaaaaataa gataaagcta tgcatgaaaa attcttctgg aagtacattg atgattggat
     5101 cagtaacggg atcgaataac cagtcatcgt tgttgaaaaa cgtttcatga aaaaggataa
     5161 aaaagcgatc aaatcctatc atcatgaaaa aaccgaaaac aactggcagt aacatgccga
     5221 tttgaaacgg acgaatcaac cgccaaagcc gatcattctt ttgcaaatat ttgataaaca
     5281 aaatactcgg gatcaaagtg atgaaaaaca cagcataatc cactaaaaac aaaattttca
     5341 cttcataaaa gtgatgggct ccgctagcag aaactgggaa atctggcaag ctgaggatgg
     5401 ttttgaaggg attattgaga taattcatta ggtgatcaaa atttttcagt aacgtctctt
     5461 gatctaaaga agtataatcc aaaatttgca aatgtccgat atcaaatacg tatagtggtc
     5521 gaaagttgat cgtcacacca actgccagac tgatcagtgt cagaaatagg ctgaacaatc
     5581 cagcatattc catccactgc catctcgaat ttttcatatt agaagctcca ttcatccagt
     5641 gattgtttca cataggtagc tgggataggc aacccaggta catcttcttc ttttgtaaaa
     5701 cctgataaaa cgagcaaagt atctatatta ttgcgtatcc cagcttggat atctgtttca
     5761 tagttatctc caaccatgat tacttcttct ttttgcagcc ctaatacttc aacggcttta
     5821 tccataatga tggcttctgg ttttccgatg tagatcggcg gcgtttgggt tgctgtctct
     5881 acaaaagaga tgactgaccc tgctccagga agaaggccgc gttcagtagg tatattctta
     5941 tctgggttcg ttccaatgaa agtagcacct ttttgaatag caagtgttgc tttaactact
     6001 ttttcatagg tcagataatt atctaagcct accactacat agtcaggagc ctcttcttcc
     6061 catgtaaatc cggcagctaa aatcaagtca atcaatcctg cttctccgat aacatagact
     6121 tttttccctt ttccatctgc tttcataaaa tcgattgttg ccaaagtagc tgtgtagact
     6181 gtctctggag ctacatgaat gtcaaactca tctgctagac gattagctac tgtttctggt
     6241 gacttagtcg tgttattggt aacaaacaag aaaggtaatt ttcttttttg caattcttcc
     6301 acaaatcgtt taccggccgg tatgggttgt gtcccacgat aaatcgttcc gtctaaatca
     6361 attaaatatc ctttatagtc cattcatcca ctactcttcc ctttttctga tcgtaaatgc
     6421 tgtttttgtt ttttctactt gtttaggttg ttcttgtttt tttacttttt cagaggtttt
     6481 tgctgactct gtttttcgat ttttaataac cggttttttg cgattagatg cgactggtcc
     6541 ttttttctca tcgatatacg cacgattatg gttttttttc ttttttcggc gctgagttgt
     6601 cttttctttt ttaccgccga ttctctcgat gacaaaatac gcacaaccaa aattgcaata
     6661 ttcgtaaaga tagtcttcca aggaatcgat tcgctgatct ggatgcgccc gtcgattatc
     6721 cgcttggaaa aatcctttta ggcgtagttg ttcataaccc caatctccta cgatataatc
     6781 atatcgggct agtacatcac tgtaacgttc tccaagcttt tctgcattaa atccttcacg
     6841 ataatcggac acaagccgat aatgtctttc acctatcaaa aaattactgt catctaacag
     6901 tgtcactagc tcgcctttga ctttttctgc tttaggctcc tcaacgattt cttccaaagc
     6961 agctgtcagt tcttcggtga ctgttgtttc ctgttcagct gatgattttt tcttagcaat
     7021 ttctgtttta tgctcaacgt tctgctgttc attttgtttt gcagacatgc gttcaccacc
     7081 tatctttttt attatacctt gtttttattt gattgggtag tgagctttca agtaatctcc
     7141 taccacactt cggataaatt cagggaacaa aaattcattt tttccagcaa tctcaatcgt
     7201 tggaaaaaat ggaacaaaca tcaaatgatc tacggtagta aaagtataat ttttattcag
     7261 atcgattggc ttgttcttcc aaaagacttg atggttaata gaatcatatg tgattccatt
     7321 atagaccatc tgcccaaaaa tcttaccgcg aaatcccatt cccttcatag gatagttacg
     7381 taaaaagtta cgattttttt ctatttctaa aactaagcgg atcagatcat ttccacccaa
     7441 tgtgacattt aacaagtgca tcggatgcgg caacgcagtg tgaagctgat cctgattgac
     7501 ccgccctttt ggaatttcag ttaaaaacag accactgttt aatattgctg cttccgtctt
     7561 tcctcgttct ttcaccgcct caaaggctac ttggataaac gaatgctcat tgaagatatc
     7621 tagactcaga gaaaatggca aatctgctac ttctttttct tttaataatt catgcccacg
     7681 ttctagataa ctatttattt cttgttcatc ttctgtaaaa gctgtcattg tttcggtagg
     7741 gatggctttg gctgaatgct taaggatttt tttctcttca tcgatggtta aatggacttc
     7801 accgacatac tgcccgtact ttccagcggc tgctatttgg acatcattga ttacgtgacc
     7861 ttctctgaat aaatgatggg tgtgcgatcc taaaataaca tcaattgctg gtatttcttt
     7921 tgcaatttgg aaatcatcct gaatcccgag atgactcatc aaaactaaaa catccacttg
     7981 tgaacgtaaa tcttcgacta attctggcaa aatatcataa ggattccgta tgtcccagcc
     8041 attcggatta taagtaagcg gaaatggagc agtaaatgca attagtccga ttttcgtctg
     8101 gtgtttcgtt gtaatgattt tatatggttt cgtccatgcc ggtggctgga gcgtattttt
     8161 atcgaataag ttatccagga tgatatcaaa ctcagcatga tcgtataaat gattcaactc
     8221 ttctttggaa tttcctacgc cttcattgtt tcctatcgtc gctgcatcat aaccaacttc
     8281 attcatcagc aagatatttt cctgtccatt cgttgcttct gacaatggat gccaacgatc
     8341 cacaaaatca cctagatcta ctgcaatcgt tgtcgttttt tctttctttt cgttttctcg
     8401 tttacgttga tccaaaaacc ttctgatcct tggccaattt tctaaatgag aatgcagatc
     8461 attcgtatgt aaaatcacta ttttttccat gtaccctctc gcctctcttt atctttatca
     8521 taccataggc agctttttct tccattgaaa caagctctat ttcagagttt ttcctgcctt
     8581 gaagcaaatt gtataagtac aatattcgat aaatcagtct ggctaaaaaa tcaggaacat
     8641 tcatttttac agtactataa aaataaaaaa aacagacccg aaagtctgtt gtcgtttcat
     8701 tataatcaga cacatggcgg aacctgctta gtgctgcttc cttccagacc tgacacgctt
     8761 cacaagccca ccattgtcct agcctttcgg caaagaatag tataccttat ttttcattcg
     8821 ttgaccagtc tttttttaat ttcttttttc gctggcggag ctttcggaaa aagtcagtca
     8881 aaatcatccc acattcgtct tctaaaatgc caccttctac atatgcttgg tggttaaacc
     8941 gctcgtcctc caaaagattc atcaaggtac cggctgttcc gccttttgga tcatacgcgc
     9001 cgaaataaac ttctgctaca cgagacaaaa tcatcgcgcc gctgcacata ggacaaggct
     9061 ctaaggtaac aaaaagtgca gcatcttcca agcgccagct tcccactttt tcacaggctt
     9121 ccttgattgc aagcatttct gcatgtgtag tagcatcttg cgtggtttct ctaaggttat
     9181 atcctcttcc aatcactttt ccatccaata caaccactgc tccaatcgga acttcatgaa
     9241 gcatttctgc tttctttgct tctgccaatg caaggcgcat ccatttttct ttttctactg
     9301 catcaagatt tgtatttacc aattaccctt ccctgccttt gttttttctt gttttacttt
     9361 agcatatgat aaaaaggctg taactaactt ttttttctat ggtaaaataa atacaagagg
     9421 tgaacaagat gaaaatggat aaattacttg ctctttaccc acaagcacag aaaaaaaact
     9481 ctccagcatc agaagcggat atcctttctt ttgcaattga tgatgctttt atctggatca
     9541 aacaagattc attatctcaa caagaaacca gccttttgaa agcgttattc cctgtaatca
     9601 acgatcaaaa aaaacaccca tggtatcgct atctttttaa agatgcttct tgtgttcaag
     9661 aaaaaagctt tcgtttgatc cagcttcacg tcgaatcaaa aggagaattc ttaaaaaaag
     9721 aatggcaaga tactattttg gaattatttc gctattctgc tgatttcttt ttttatacag
     9781 acacagatgc gctcttagtc gaacaaataa accgtgatta tcttgatgct actgaaatca
     9841 acggtatttt tctatcttta gatgctgatt ttgatacgac aacgtctgta ttcgttggtt
     9901 ctttccattc acctggcacc gacctcgtcc ctttgtttgc tgaagaaaga aaaatttttt
     9961 taacagagca aaacaatctt tatactcaca gccgtacttt ttctatgcaa gatgtggctt
    10021 tacattacta tacaaaagat actgtaaaag atagtaagtt gatcaaaagt tacatagaac
    10081 tgatcaaaca aaatgaaatg gaagtgatca ttcaagaatt atggaatgaa ttaggcaatg
    10141 tcagctctgc tgcaaaaaca ctgtttcttc atcgtaatac tctaaaatat cgaatcgaaa
    10201 aatttcaaga acagtctggt ttcaatctaa aaaaagccaa cgatttactg ttttgctatc
    10261 ttttattgct tcatgaacat aattaaagat agattaaagg ggttgtgaca ataattttgt
    10321 cacaacccct tatttcgaat gaatagtgtt caaaagctag tttctcttac atcatttttt
    10381 caacaaaatg cattagccgg tcttttacat cttcggttga atctttgatt gaatcaccat
    10441 aaacagagat tttctttttt cctgatttga tttttgctaa cattcccatc aaagaatcca
    10501 agtcactatc tgaaagacca tctacgattt ctagtaattt ttcatttcca tcaaatttgt
    10561 catagacgaa tttttttact ttcgtccgag tagtcatatg atggatcttt tccattactt
    10621 ttcccgatac aacgactgct gtcgctacac tggcagcggc tgctacgctt aatccgattg
    10681 ttacttttgt tgatgttttc ataagtatca gctccttttt atctatctcc atcatagcat
    10741 agacaaaaaa ataaacgaac taatccactc agtgaaatct caatccttta gggaaaaagt
    10801 tctttactgt tccttttact tgtttcccaa atccgacagg tatcttatct aatactagca
    10861 aaacccatcc ttgattccca tcacgttgaa atgtttctcc tgctgtgtaa gattgccatt
    10921 ccttttgagt gatcggcaag caaggtatat tctctatctt ttttgtagca agcgctaatg
    10981 catagctagg ttcaaatcga ttctttttga aatctcctaa atgcaaacct gtacgtacga
    11041 cttttaaacc atcgagagaa ggggctaact caggaacttc ccaaagatgg tcgttgaaca
    11101 ctaaaagtct tccagtagct tcataatgaa aatcatttga aaactccgtc cagagttttt
    11161 cctgctcttt tgtcatttgc actttacttt tttttcgtgt cttcttttct ttatgcatct
    11221 gattttgtcc atgaaaagtc agctttgcaa caaaatgtcc ttcaccctga tctttatgag
    11281 gccatatacg tattgttttt tccaaaccag ctacgcttcc ccattcagaa cgtcctgaac
    11341 taacggattg agtcaaagga atctcctcaa tcgttactgg ataattttct acaagccagc
    11401 taatgatctc ttcattttct tctggtgcaa acgtacaagt cgaataaatc agttgtcctt
    11461 tattttttag catttttatt gctgaagaca aaatctcttg ctgacgtttt tgacagtata
    11521 gcggagattc ttctgtccac tcttttattg catttggatc ttttcggaac attccttctc
    11581 ctgaacaagg agcatcaacc acgatccgat caaaaaagcc cgaaaaatga gggacaagtt
    11641 ctgcgggtgc atgatttgtc actattgcgt tcgaaactcc ccatcgttct atattttctg
    11701 ataaaatctt cgcacgtttc ggaaagatct cattcgtcac taataaccct ttccccttca
    11761 tttgagcagc taattgggtt gatttaccac cgggagccgc acacaaatcc aaaacttttt
    11821 caccgggttt tgctgcagca gctgttccaa cgatcattgc acttggttct tggctatatt
    11881 catacccagc ttgatggagg aaagactttc cattgacagt gccaagaaat ccctcattgc
    11941 tgtagggagc gggttgtaat tcttcgctat ggtaagtttg gaccatgtcc aatcctgccg
    12001 gttttagtgg attccaccga aatccttttt tcacgcttcc ttgttcaagc gcagaaaaaa
    12061 aatcggatgc ttcttcgcct aaaagaagac gatatttttt gataaattgc tgtggtaacg
    12121 ttgtcgcttc ctccttcatt ccaatgtttc gttagacatt ttacaatctt ttttacgaaa
    12181 aataaaggtg gctgatctac tttttcatgt tgctgcctaa ggtgactgtt cctttaagat
    12241 attttccaag tattcatccg tcacctcgtt tttatcccaa acagcgttca tcatttcaag
    12301 aaaatcttga gaagaatcat ctgagcgcaa acttcccaat gcattaagcc ataccaattc
    12361 aagcgacttt tcttctttga tcttcgtgtg acctttgaca tatgagaaaa atggccgctt
    12421 aaatagcgaa tccatgtcta aatcagattt tgcaatgatt tcacgaatac tttcatggat
    12481 agaaaaaaaa tctttcaata agtagacaat gcttaaaaca tcatcggaac tgcattcagc
    12541 taagtagttt tcaaaataaa cagtgatttc ttctaatatc ttctctttta acccgctgat
    12601 tgatttgaaa tgtgtataaa tcgggtatgt agaacaaccg cagaactctg ctaactctct
    12661 tgcagtaatt gacttcattc catattcttc cacgtatacc atacttttga acagcaaata
    12721 ttctttacta taatttgtta ctctccccat cattcttacc tccgtctctt ttcatctacc
    12781 cataaataaa taacataaat aattctaaaa atattattga taattatcaa aacatattta
    12841 gttatatcca ttaaaaatca atttttccgc cttaaaaaca agaatatctt gacaaaatac
    12901 cctaggaaat aaacaataga ataaacaagg taacaaaaaa attaaataac gttatataaa
    12961 aaagaaggcg acactctgtg agaataacac agactgaaat gactttccac tcacagtttt
    13021 tgtctaacta tatacaaaaa cagaataaaa aggaggtatt tatgctaatt gaagcaagaa
    13081 aaaaagagtt acttgttcta caaatggtca ttcaaaaaaa taattgttct ttacaagaat
    13141 tatccaatga cttacatatt ccaaaacgga caatcaaaga actgatccga aaaataaatg
    13201 tatcactcaa gcaattttta ttgattgatg aatttattta ttctaatcat aaaggagaga
    13261 tccaaaccaa agaaaccgat caaactaaaa agttagctgt tttttctaag ttaaaattat
    13321 gctacctaaa agaatccaat cgttttaact atttgatgtt actaatcaat tatccacaaa
    13381 ctgctgtacc aaaaaaatat ttacttgaac aactttatat atcgccctcc tatctcgaaa
    13441 aactgacaaa acagctcaat cattccttaa aaaaattcca tttacggatc ctatctgtaa
    13501 aaaattgctg tactcttgaa ggaaatgagt tatctatccg tctctatctt tacttttttc
    13561 tttcagatac atttggaggt aacgattggc cattttcctc acttgaatta aatgcactaa
    13621 aaatgaacag tacagatgag caaatagaaa aaatttcaaa acaagaaaaa aatatctact
    13681 tgctgttaac tttattcata ttacgaaaca aacaacaggc atattttcca aaaacttctt
    13741 ctgaaatcac tgaattgatg gacttattgc agcatacaca tgattattca gaatacttta
    13801 cttctttttc agtgagtcgc ccttgtgaag agacagaaac agagagagcc tactttaatt
    13861 attttttcta ttgttttttt ccaaatcaaa tctcttttcg acaaaaacag gagattggtt
    13921 ttttattttc cacaagcaaa aaccttattt gtcgacaagt cggtcaattg atgacttact
    13981 taactcaaca gtttcctctc aaccatttag aaaataaaat accaatttat ttttattttc
    14041 ttgtgttggc agttgttttt ttcgaactgt tgggtgaatc cagcagtagt ttttctacac
    14101 tttgctttcc agaagccacg tccaatcatt ctagttcttt tatacaggaa aagaaattaa
    14161 gagaaaaaat cgatacaatt ttttcaaaaa atggggaaag aaaccaaatc agtcaatacc
    14221 ttagtcaatt gatccaacac ttactacaaa cagaaacgaa agagcagtta ctcatccatt
    14281 tgcagataac caaacagttt acaggaaatt atcatcttca aagccatttg cagcagttat
    14341 ttcgcaaaga tgctgttaaa attaccgcat attcagaaca tgctgatttg atcattacag
    14401 atcagcatga gaaaatttca gatgaaacgg agactttcta tctggataca acgcacaata
    14461 cagtgttatt tggcaagtta ataaaagcgg ttcatgaact gtatatgaaa aaaggttatg
    14521 agaacaaaac aaagaggatg tgacaaaatt cttgtcacat cctcttatt
//
LOCUS       DACDXE010000075        14352 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.075, whole genome shotgun sequence.
ACCESSION   DACDXE010000075 DACDXE010000000
VERSION     DACDXE010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.075"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(81..1817)
                     /locus_tag="IWM23_002314"
     CDS             complement(81..1817)
                     /locus_tag="IWM23_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAP9884921.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(1814..2518)
                     /locus_tag="IWM23_002315"
     CDS             complement(1814..2518)
                     /locus_tag="IWM23_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9884922.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            2649..3152
                     /locus_tag="IWM23_002316"
     CDS             2649..3152
                     /locus_tag="IWM23_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAP9884923.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            3112..3450
                     /locus_tag="IWM23_002317"
     CDS             3112..3450
                     /locus_tag="IWM23_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAP9884924.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            3471..4889
                     /gene="ffh"
                     /locus_tag="IWM23_002318"
     CDS             3471..4889
                     /gene="ffh"
                     /locus_tag="IWM23_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAP9884925.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            5001..5579
                     /locus_tag="IWM23_002319"
     CDS             5001..5579
                     /locus_tag="IWM23_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAP9884926.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            5583..6104
                     /locus_tag="IWM23_002320"
     CDS             5583..6104
                     /locus_tag="IWM23_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9884927.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(6183..6737)
                     /locus_tag="IWM23_002321"
     CDS             complement(6183..6737)
                     /locus_tag="IWM23_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9884928.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            6990..7265
                     /gene="rpsP"
                     /locus_tag="IWM23_002322"
     CDS             6990..7265
                     /gene="rpsP"
                     /locus_tag="IWM23_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAP9884929.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            7277..7528
                     /locus_tag="IWM23_002323"
     CDS             7277..7528
                     /locus_tag="IWM23_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAP9884930.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            7653..8444
                     /locus_tag="IWM23_002324"
     CDS             7653..8444
                     /locus_tag="IWM23_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884931.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            8614..9135
                     /gene="rimM"
                     /locus_tag="IWM23_002325"
     CDS             8614..9135
                     /gene="rimM"
                     /locus_tag="IWM23_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAP9884932.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            9125..9886
                     /gene="trmD"
                     /locus_tag="IWM23_002326"
     CDS             9125..9886
                     /gene="trmD"
                     /locus_tag="IWM23_002326"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAP9884933.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            10022..10369
                     /gene="rplS"
                     /locus_tag="IWM23_002327"
     CDS             10022..10369
                     /gene="rplS"
                     /locus_tag="IWM23_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAP9884934.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(10501..11637)
                     /locus_tag="IWM23_002328"
     CDS             complement(10501..11637)
                     /locus_tag="IWM23_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884935.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(11753..12667)
                     /locus_tag="IWM23_002329"
     CDS             complement(11753..12667)
                     /locus_tag="IWM23_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HAP9884936.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(12758..13186)
                     /locus_tag="IWM23_002330"
     CDS             complement(12758..13186)
                     /locus_tag="IWM23_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9884937.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(13191..13565)
                     /locus_tag="IWM23_002331"
     CDS             complement(13191..13565)
                     /locus_tag="IWM23_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884938.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            13851..14036
                     /locus_tag="IWM23_002332"
     CDS             13851..14036
                     /locus_tag="IWM23_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9884939.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            14048..14296
                     /locus_tag="IWM23_002333"
     CDS             14048..14296
                     /locus_tag="IWM23_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884940.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
BASE COUNT         4647 a         2573 c         2789 g         4343 t
ORIGIN      
        1 taaaaaagaa caaccactcg tgtatctttc gaagcaggat gagtcatcca actttaaaac
       61 gcacgagcag ttgctctttt ttacattttc ggaatggtaa cggtgaaagt cgaacctgtt
      121 ccaagatgac tgtctacagt aatcgtacca ttaaggagtt gcacgtagtc ttttacaatc
      181 gctaacccta atccggtccc gcctgaatgt cggcttcgcg ccttgtctac gcggtaaaaa
      241 cgctcaaaga tccgttcctg atcctcaatg tcgataccta taccaaagtc ttggacagaa
      301 aacgatagat catctgttgc ctgatagcgg ataataatct cgctgtcgct ttttgaatat
      361 tggacagcat tttcaattag atttttgata attggataaa ggatatctgt tttagtagta
      421 aatggttcgt tctttccaat caatcgaatc gtcaaccgtt tcttttcgat gattggctga
      481 taattttgaa cgatttgctg gaaataaggt tctgtataaa ttgtctggat ctcgtaagaa
      541 ttctcgccat cttttgacag ctggatgatt tcccgaatca gtttatctaa ccggatcgca
      601 tctttttgca tgatctcaag aaaagaagtc agagtttgcg ggtcatcttt tgcaccatcc
      661 agtaaagtct cggtaaaacc aatcagtgaa gtcacaggag tttttaattc gtgagagacg
      721 ttgccaacga aatccttttg tagcttttct aaacgtcgca ctttcgttaa gtcatacgcc
      781 acccctagaa tctgtccggt accttctgaa ttagtaaagt agcgcaaact caagtctaat
      841 gtctgccgac cttctgtaag ggtgatctcc tgatggatca gcggatgctc aggagtcact
      901 tgatgaatca gctgaatgag ttttggctct tgaatgactt ctgtgtaccg ctgttcaggt
      961 tgatagctta ccacgtttaa atgtagctgc atctttggat taagcagtag taaacgagaa
     1021 tcagcatcat caatcaggaa tacgccaatc atcagttcgt tcagtaacgt atataattgc
     1081 tcttctgtgg tagtacacgc ccgatacatc ttactcattt gttcgctcaa cgcattaatc
     1141 gtctgataca attcttccca ttgatcgggt gtttgcatga ttacttccgc ctgattagga
     1201 ttttgcacca ttttttttag tacgggcagc acggttttca atggctggtt tttttgatag
     1261 atcagataat agatcattcc tgcaagaaca aggaagaaag acagaaagaa gaaaaagacc
     1321 caacggcgaa aactctcagt tctcggtaaa aaaccgcttg tcggttctgc gatacgtaaa
     1381 atggcttcta actggccact ttttttgact ggtaatgcga catataaaag atcctcattc
     1441 aaagtagtac ttttgcgcaa agcgctacct aggttcccac cattcaatac ggctttgatt
     1501 tcgggacggg agtttcgctg ttcattcaat gtttcattgt ttgtgtcaaa taagatcttt
     1561 ccagtagcat ccattaaagt aatccgttcg ttcgactgat ggacgaattc ttccaaagca
     1621 gttttgtttt gcgctgcttg gaggttttcg acatctaatt gccggatcag cagttgtgct
     1681 ttttttgtca ggtagctttc ttgctgttcc acgatctgct gttgaaaata atgggaaatc
     1741 aattgccagc caccaaaaaa caaactaact aaaagaataa gaaagaagag acgttcataa
     1801 atttttttcc atttcatcgt ttgggttcct gaaacttata gccaaatcct ctaatagtca
     1861 ccaagtactt tgggtgtttt ggatctcgct cgattttttc tcgcaaatga ctaacgtgta
     1921 catctacgat tcggctttgt cctgcgaagt caaaattcca aatgcggtct agcaatgtgt
     1981 ctcggtcgat cacacggtct tttcgtttca tgaaataaac aagtaattca aattctttcg
     2041 gtgttagttc aatcagatgg tcgtctacgg tcacttgata attggtcaaa tcagcagtga
     2101 tttgtccaat cgatagatat tcaggctcag cttcatcttt tgattgctta cgaggttcaa
     2161 ttcgtctgaa tattgctttc atacgggcta atacttcgcg aggactaaat ggtttggtca
     2221 aatagtcgtc agcacctatt tctaggccaa tgatccgatc cacttggtca tctttagccg
     2281 tcagcatcaa aatcggtgtt tcattttttt cttgacgcag ccgctgagtg atctccatgc
     2341 catccatcgt tggaagcatc acatctaaaa tgatgaaatc aaatgattgt tctaatgcta
     2401 attctaatcc ttcgcctcca tctgtggcac tggttacctt gtagccttct ttttccaaat
     2461 tgaacgtcaa gagcgttaca atagaaggtt catcatcaac aactagtatt tttttcattc
     2521 aaatctcctt taaataggtt tcattctgtt tatattttag catagaatca gtacaaaggg
     2581 ctattttaca aaagaaataa atggtataat aggaaggcac tttgctgaat agaaaggaag
     2641 agcagtcaat gattggaatc agtgcatgtt taggcggtct ggcctgtcgc tatgatggac
     2701 agaaaaaaga agtaccagaa ttgatggaac tagttgaaac tggagaagct gtcatggtct
     2761 gtccagaagt gatcggcgga ttaccaattc ctagagatcc cgctgaaatc attggtggaa
     2821 atggattcga tgtgtggaaa gatcaagcaa aagtatggac gacaagcggc gaagatgtaa
     2881 cggaagcgta caaaaatggc gcgatcaaag cctatcaaaa attgcaggaa aaacacattg
     2941 atctagtcat catgaaggaa aaaagccctt catgcggaac ccattcgatt tatgacggaa
     3001 gtttttccgg aataaaaaaa aatggtgccg gagtggctac tgcttatttt attcagcaaa
     3061 aaatgactgt gctctctgat gaagaatggc tgaaaactag aggtgagcag aatggaaatc
     3121 gaaaagacca atcgaatgaa cgcgctcttt gaattttatt cgaccttatt gacagaaaaa
     3181 caaatgaact acatggaatt atattatgca gatgattttt cattaggtga gatcgcggaa
     3241 gaatacgaag taagccgaca agcagtatat gacaatatca agcggacaag caagatcttg
     3301 gaggattatg aaaaaaaact ccatcttttt tctaattata ttgttcgaga acaggtattg
     3361 gaaaatatga ctgcatatat tacagataag tacccggaag ataaaaaact acttgaatat
     3421 gtacagcaaa ttcaagcaat cgaagaataa ataaaaggaa tgaagaaaat atggcatttg
     3481 aaagcttaac tgaacgtctc cagcaggcga tgggaaaaat aagaaaaaaa ggaaaagtat
     3541 cagaagctga cgtaaaagaa atgatgcggg aaatccgcct tgccttacta gaagccgatg
     3601 ttaacttgca agtagtcaaa gattttacta agcgcgtacg cgaacgtgcg gtaggggcag
     3661 aagtgctgga aagtttatca cctgcacaac aaatcgtaaa aatcgttgat gaagaattga
     3721 cgatcacact tggttctgaa acagcagagt taaataaatc accaaaaata ccaactgtta
     3781 tcatgatggc tggtcttcaa ggggcaggta aaacgacctt tactggtaaa ttagccaatt
     3841 atttaaagaa aaatgaaaat gctcgtccgc tcctaatcgc aggggacgta tatcgaccag
     3901 ctgcgatcga ccaattgaaa gtattaggtc aacagctaga cgttcctgta tttgatatgg
     3961 gaactgaagt cagcccggta gagatcgtcc gtcaagggat ggagttagca aaagaaaaga
     4021 aaaacgacta tgtattgatc gatacggcag gacgtctgca tatcgatgaa acattgatgg
     4081 atgagttgaa acaaatcaaa gaattgacgc aaccaaatga gattttgctt gttgtcgatg
     4141 ccatgactgg tcaagatgcc gtcaacgtag ctgatagctt caaccagcaa ttaggtatta
     4201 caggggtcgt catcactaaa cttgatgggg atactcgagg cggggcagca ctatcgatcc
     4261 gctcagtgac aggcgcacca atcaaattca tcggttctgg tgaaaaattg actgacttag
     4321 aagtattcca cccagatcgt atggctagcc gtatccttgg tatgggggat atgctgacat
     4381 tgattgaaaa agcgcagcaa gactacgacg agaaaaaagc ggaagaactt gcacagaaaa
     4441 tgcgggaaaa ttcattcgat ttcaatgact ttattgaaca gcttgatcaa gtgatgggta
     4501 tgggcccact ggaagatcta atcaaaatga tcccaggaat gaatcaagtc cctggaatcg
     4561 aaaacgtcaa ggtagatcca aaagatgttg aacgcaaaaa agcgatggtt tattctatga
     4621 cgccagcaga aagagcaaat cctgatctgt tgaacccaag ccgtcgtcgc cgaattgctg
     4681 ccggttctgg aaacagtgtt gttgaagtga atcgaatgat caaacaattc aaggaatcaa
     4741 gaaaaatgat gcagcaaatg tccaaagggg atatgaacat cccaggtatg gaccaaatgt
     4801 ttggttcagg cgtcaaagga aaactaggca aaatggcgat gaatcgaatg atcaagaaaa
     4861 ataagaagaa aaagaaaaaa cggaaataag atgaaaaaaa cagcttcggt acagactgaa
     4921 aatgtctact gccggagctt ttctatcagc aatttttatt ctttactaat acaattactc
     4981 taaacgaaga ggtggcacag atgagatgtc cgtggagtga acaaacagaa agtatgcggc
     5041 aatatcatga tactgtctgg ggtgttcctg aatatgatga ccaaaagctg ttccgaaaat
     5101 tgatgcttga tattaatcaa gccggattga gctggcagac gattttaaat aaaagtgaag
     5161 cattcgatga agcatatgat cgattcgata tcaaaaaagt cgcaaattat ggagaaacaa
     5221 aaatcagtga actaatggaa aacaaaggaa ttattcgtaa ccgaagaaaa atcgaagcag
     5281 ccgtccaaaa cgccaaagca gtcttgaaaa ttcaagaaga aatcggcagt ttttcagagt
     5341 atctttggtc gttttctaat ggaaaaatcg ttgattcttc ttatcattca caagaagaag
     5401 tcccaacaac aagtcctctt tcagatgaat tagcaaaaga tctaaagaaa agaggattta
     5461 aatttatcgg aagcacaaca atctatgctt ttttagaagc tgtcgggatc atcaatgatc
     5521 accttgacag ttgtttccgc aaagaagagg tcgtacgtat cggcagaaag ggcgaataaa
     5581 gcatgcgaaa aatcatattt tatggtgcta taagtttaga tggctatttg gcaacgaaaa
     5641 cggatgatct tcaatggctt tttgacactc caacaggtaa gcaaactact tatgaagctt
     5701 tttatgaaac aatcgatacg accatcatgg gcagaaaaac ttatcaggaa gcaaaaaaat
     5761 atttggagac cgaagcaatt tatccagaga aaaagaattt cgttttttcc acccacgcac
     5821 aattacaatt gccagatgct gaggtcgtaa acgaagatcc tgttgctttt ctaaaaaatc
     5881 tacaaatgac agaaggaaaa gatatctggg ttgtgggtgg cggtcgattg atcaaaccgc
     5941 tattggaaca tcaaatgatt gatgaatggt atatccagat tacccctgta ttattagggg
     6001 acgggattcc tttattccaa aaaggaacat atgaagcgcg ttttgaaatg ctagacacga
     6061 cacgattcgg tgaatttatc gaattgcatt atgtacgaaa gtagaagtaa ataaaataaa
     6121 aaaggcaact atatgcagaa atgtcctgta catagttgcc ttttttatta ccgttatgct
     6181 ttttactctg ttaatttgtt agaaaaatct agatacgcat ctaaaatcgt ttggaagaaa
     6241 cttaatgttc cttcaacgat attaccattt tcatcaatta gattcgcaac gtttccgata
     6301 tatgcttccg gttgctgcaa ggttggcaca ttcaaaaaga caagtgattg gcgtagatgg
     6361 tggtttgcgc caaagccgcc gatcccacct ggtgatgcag taacgactaa acctggttta
     6421 tgatcccaaa cactgtgtcc ataaggtctt gagcctacat ctaaagcatt tttcaatact
     6481 gctggcacag aacggttgta ttcaggagta acgaaaacga caccgtctag tcccctcact
     6541 tcctcacgaa aacgagtcca ttcagcagga gcctgttctg gagtttcgag atcctcattg
     6601 taaaatggca gatcatcgat ttttacaaat acactttcgt acccttcagg aagcaatttc
     6661 gcaaaagctt ctgctacttt tttgttgtaa gaatcttttc ttaaactacc aataaaaaat
     6721 ccaatttttt ttgtcatacg atttttcctc cttcaaacta tctataataa tcttacaaaa
     6781 gataagtaac tggagcaaat aaaaacactt actgacaaaa gctacataga agatagatta
     6841 cttattttta acaaaaaaga agtctaaaat cttctatttt ctaaaaaaaa tcgaatttaa
     6901 tcaagctgta aagaaaaaaa gctttacagg gtaaaaaaaa actgttacac tacatcttgt
     6961 gaataaaaca atggaggtgg aaataaataa tggcagttaa aatccgttta aaacgtatgg
     7021 gttctaaaaa gagtccgttt taccgtatcg tcgtagctga ttcacgttct cctcgtgatg
     7081 gacgtttcat cgaaactgta ggtacttaca atcctttgaa agaccctgca gaagtagttt
     7141 taaaagaaga cttagttctt gactggttat caaaaggtgc acaaccttct gacactgtac
     7201 gtaacatcct ttcaaaagaa ggcgttatgc aaaaacacca cgaagctaaa ttctcaaaga
     7261 aataaggtga ccggatatga aagatttaag cgacttagtc ttaacaatcg ttcgtccgct
     7321 agtcacttat cctgatcaag tcaagttaga cgtcgtagag tcgaaagact tttacgagta
     7381 caatctaaca gtggctcctg aagatattgg ccgtatcatt ggaaaacaag ggcgtgttgc
     7441 taaagcaatc cgcacaatcg tttacggtgt tcggatcaat gcaccgaaaa aagtgcgttt
     7501 gaatatcatc gatgataagg aaaagtaaat aaacgtgctg tactcgaatt ttcgagtgca
     7561 gcttttttgt tttttttatt aattgaagaa atgattattt ggcaaaacaa gcaagaaagc
     7621 gtatccttat aggaggaggg agtgaacagc caatgagaaa gaaaatgtta tttattccat
     7681 ttgctctatt gttaagcgct tgtagttcgg ttactcaaga atcagacaat acacatctat
     7741 caagcagtat atctgaaact gttaccagtc cagaaaaaag tagtaccaaa acggcttcca
     7801 ccactgaaca aacaatggct acctcaaata atgagaaaaa gactgcttta gaccagttga
     7861 aagaacaaca gccaaatgtt cccatgcctc tagatgtacc agtctcttca ggctatctca
     7921 atatcgcagc gacacataca aagcaaggtt attctatttt atattacaga acagatcgtc
     7981 ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc tattgcaacc tatctatatc
     8041 aatatgggtt tgcttctagt caagaaacaa tccaagtttt gcagccattt gagatcgaca
     8101 caaatggtca gcaagtggac ttaggttctc gaatcacagg gtatcagcaa ggtgcagcag
     8161 gttcaagttt tcttgaatgg caagaaggaa actggcgtat tcgaatccgt gggaataata
     8221 tagaaggcca agatccctta ctattagcta aagaaatcgt agcttatttg gaagaaaaca
     8281 gtctaccagc acctgaacaa tttggtaaaa ttactgttga tatgggtgat acgacgaatc
     8341 gagccgtaga agtaagctgg caagaaccga aaaatgccta tacgatcact catcaagatc
     8401 ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt gtgaaatcag cgcgctgatc
     8461 tgagcccatc atttaactcc aagtcacagg aagtaatcaa aaatagcacg ccgcattttt
     8521 gcataatttt ggcttaatac cttaaaagaa aactagaatt gcctcttcac acatgctaaa
     8581 ataaaacaga caaaaaagaa atggggatat aagttgactg aatacttgaa tgtaggaaaa
     8641 atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta tctcaacaac ggatttccca
     8701 gaagaacgtt acaaaaaagg aacggtgcta acactattcc aagacggaaa agcacctgtg
     8761 gaattaacag tcaaaagtca tcgcaaacat aaaaattttg acttattgag ttttgaagga
     8821 catccttcta tcaatgatgt ggaaaaatat cgggatggca tattaaaagt gtcaaaagac
     8881 aactctgtag atctagctga aaatgaattc tattaccatg agatcatcgg tctgcatgta
     8941 ttagaagatg gaactgagtt aggtaaagta aaagaaatct tgtcaccagg tgccaatgat
     9001 gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc ttccatatat cgcttctgtc
     9061 gtcaaagaag tcgatttaga agctggagtg gtccgtgtag aaattccaga aggactgatt
     9121 gacgatgaag attgatgtat tgactttatt tccaagaatg tttgaaggtc ctatggggga
     9181 atccatcatc ggtaaggctg tagataaagg attattagat atcaatatct ctaacttccg
     9241 cgattattca gataacaaac atcagacagt agatgattat ccatacggcg gtggagcagg
     9301 tatgctgctc aaagtccagc ccatctatga taacatccaa gccattgaaa aagctgcacc
     9361 ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt aaacgattcg atcagaaaat
     9421 ggcagaagaa ttttcacaag aagaacactt agtattcatt tgtggtcatt atgagggata
     9481 tgatgaacgt atccgcacat tagtgacaga tgaagtttcc ttaggagatt atgtccttac
     9541 tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca gttcggcttt tgccagatgt
     9601 gttagggaat cagacttcag ctcaaacgga ttcttattca accggtttat tggaacatcc
     9661 ccaatatact cgcccggcag aatataaagg gatgaaagta ccagaagtat tgacaaatgg
     9721 aaatcataaa ttgattgaag aatggcaatt aaaagaatca ctaagaagga cctatcaacg
     9781 tcggcctgat cttttggaaa cacttgaatg gacacctcaa atgacgaaat ttttagaaga
     9841 aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc aagtgacttt ttctaaaatc
     9901 ttctttacag taggttgata atatgttagt attttatggt gagtgcaaaa gcacttaact
     9961 attacgatat tccgctgtga gaaaagctca taagaatatt tggaataagg agaagaaaac
    10021 catgaatcca ttaatcgaag aattaacaaa agaacaatta cgttctgaca tcccagcgtt
    10081 ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt gaaggtactc gtgaacgtat
    10141 ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct ggaatcagcg aaacttatac
    10201 agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca ttcccattgc atacaccacg
    10261 tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt cgtgcaaaat tgtactacct
    10321 acgtgcatta cacggaaaag ctgctcgtat caaagaaatc cgtcgttaat catcgttatc
    10381 agactgagaa agaacccttg gtattcaagg gttctttttt tgttctctgt ttttttatat
    10441 taacgatttc tatttgggac atttttgatt aataaaaagg ggcaaaaaag gggcaacaag
    10501 ttacagtttt tctatatctt ctttcaattt ttcttttact ccctttgtca catgcaaata
    10561 aatcttttca gtgatatcac tattttcgtg cccaacgcga tcctgaatag catacagagg
    10621 agttcctagt tccgctaatt ttgaaatatg ggtatgtcta aaaatatgtg aactaagttt
    10681 tttatcaatc ttcatgtcag ctttatgatt tctcaaatag gtgttaattg ctgttagttg
    10741 gaaaggagtt cctttcgttg tttgaaatag aaattgacca ttcggattta attctagaag
    10801 ctcattgtaa atagctatcg ctttttttgg taaatcaatt tctcgcattc cagcagcagt
    10861 ttttgtagaa tcagattttt tcatatcagc tattgaacgt tctctataca tcatcgtccc
    10921 atttataact actgatgcat tattattggt aatgtgtaca tcgtctttac ttaatgcgat
    10981 tgcttctcca ggcctcattc cagtcaaata tagccactgg aaaaggagag aatatctttt
    11041 attgtgtgaa gttgtgaaat ttactagtct tttatattca tcatcttcta aaaacttatc
    11101 tttaattttt atcgtttttg actctcgttt ataatcaata actacttcgt tgataggatt
    11161 tttttctaca tagccttttt tcatagcgta tgaaaaaaga agattcaatt tggatttaat
    11221 tacactgacg tacttatttg acaagtcatc tttatatatc atatcttcaa aaatattatt
    11281 taaatctact gtattaatac cagaaacgat gtaagtctca ggtatctttt tctttatagt
    11341 gtttaagata ttattcgtag gataatatgt agactcctta acttgtcttt tgtaaataac
    11401 taaccattct tcgacaagct catgaaatgt tagatcaggc ttctgaagtg ttttttcgtt
    11461 taattttata tcaatttttt tatttaactc tagcaatgcc actttctgag tttctcgaga
    11521 tttattttta taagtaatgc tcactttttt tctttttcta gtttttggat caatatatcg
    11581 ctcattatat ttatatactt tttttccgtt tttatcagtt ttggtttcaa tccacattgt
    11641 tttcatctcc tatttgttgc tataatagga atggataagt aagccaatta tagcaggttt
    11701 atttttcatc acgtccacaa actttggcga gggagggggc gtgttttttt gtttagtagc
    11761 tacctttcca tgaagtatct gcgaagaaca cttcaaatgt tgttccatca tttttcactt
    11821 caaaatatgc tgttccttgt gcggatttac ccggttggat ggtttccgaa aagtaatctc
    11881 tactagctaa atcagattta atatctgatg aatcataaaa ttctaaataa cttgaacttg
    11941 catcaaaagg tgtagtccct gtgttttcta caagaaaatc tacctttgcg taaaataggc
    12001 cttctggttt atagaaatca tcaccgtaat cttttgatac agactggaca gttacgttaa
    12061 tagagttacc attttcagaa gtaaaaggaa ctgcttctcc tatactatgt gcaactgatg
    12121 ccgtctgggg ttctgaagac gaagttgtag ttgtagttgt agtagagctt gaatctttgg
    12181 attttccatc tttatctaag aaagttcgtt gattataata tacaacctgt tcagtttttg
    12241 gactaaccca aattaacatt gtttcatcat cagaatcagt tgaataagta tattggagca
    12301 tctctaattt ttttatatct tttgcactat ttccatgttc aattaattca gagtgctctt
    12361 tataataatc tccttgtgca tcctttgaaa tagatacaat taattcatcc cgtgtgatag
    12421 cattgtctaa ttctttgtaa acaaaatcat ttgtccattt acgtaacggt gaaccaagtg
    12481 tctgataaac ttgagttaaa tcatcaccaa cttttatttt atgaacgtct gatgaagtta
    12541 gtttttttgt ttcatattca gatgttgatg aaacagtatt ttctttagtg ctagcagaat
    12601 taggactagt ggagcatccg cttaatacca agcctaaaaa aagcaaccca aaaacaattt
    12661 ttttcatttt taattcctca cttctatgat atgatttttt tgtaggatct tagaaataag
    12721 gtttttagtc cgtgttgcag cacggacttt ttttacatta tataagaaag cgataagcgc
    12781 tttctggaag tccgtaaaga ttctttaatt cctcaattcg tttaggatat tgatcattgt
    12841 cttctttgta tagagaaaca atgagattag cagcaaagca attagcttcg ctttcagatt
    12901 tgcttctaga tgttcttgtt gatacatagt aactggataa gccacgatga aaaatagcgt
    12961 gacctaattc gtgagcgcaa atgtagaatc tttcctcaga gtctctcagt tcatcattta
    13021 agaagattat tgcacgacct ctaatttctt gaaactgtcc tttgggattc tcgataaaag
    13081 gaacgtattg aattttaatg cccatctttt cacaaatata aaaaggatta gcggactggt
    13141 atttccgctt caactcctcg actaaattaa tcgtatccat ctccataagc tcacatcttt
    13201 ttgccttttt ctttgtcttc tttcacaata tcccagaagg ttccaataag gatatctttt
    13261 acgcgttgga cctgttcagg cgtcagtgtt tctccgccat aagacatatt gacatttgaa
    13321 tctagtaatt tatcaagttc aactacttca tcttttgttg cccagtcagg aacttggttt
    13381 ctaccgagta aataatcagt agttacatta aaataatctg ctacaagttg tagtctttta
    13441 gtactaggag ttctattttt ccattgataa atcgtgtttt taggtatatt taattcttct
    13501 tccaattgag ttatacttat gcctctttta tgagctaatt cctttattct atctagtaaa
    13561 ttcattttcg cacctcacaa gctacgaaaa cactaataaa aaaattagtt aaaggtgttg
    13621 acaactaata aaagtgttag tatactgttt tcgtaagcta aaatattagc taaaaagact
    13681 tgataactaa taaacacttc acggtcggca aactctgaaa tgtaaattac taggcatttc
    13741 tgtgtcttat ttagctatgt ctatatacta ataaaaatat tagttattgt caacgaatat
    13801 tagctaattt tttagcttac agattatttt tttagaaagg agctattttt atgtctgaga
    13861 atttagactt aaaaattcga gcggagatga gaaaaagaag aatgactttc aaagaactag
    13921 ctgcgcttat cggtatttca ggagcttatt tatcagatat tctaaacggc aatcgtgatg
    13981 gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat attggacatc cgataggggg
    14041 gtaggcgatg acaaaactaa aaaaacaaga ttttgtaaaa aaatacaatt attctccatc
    14101 tacttatcaa cgtcgaatgt cggaactaaa aaatacagca attttctcag cggcgtatga
    14161 acgggtcaca ggacaagaag tttggatcaa tacagaatta tacgataaat ttttgtcttt
    14221 caaatcctat aacaggttac gcacaagaaa ggtaacacct aaagaattta tcgagaagca
    14281 tttagttgat ttataaaaaa cagaacattt tgagaggtga aggttaatgg gtaaattcaa
    14341 cagagcattg gt
//
LOCUS       DACDXE010000076        14041 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.076, whole genome shotgun sequence.
ACCESSION   DACDXE010000076 DACDXE010000000
VERSION     DACDXE010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.076"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWM23_002334"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="IWM23_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAP9884941.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWM23_002335"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="IWM23_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAP9884942.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWM23_002336"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="IWM23_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAP9884943.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            1642..2274
                     /locus_tag="IWM23_002337"
     CDS             1642..2274
                     /locus_tag="IWM23_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAP9884944.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="IWM23_002338"
     CDS             complement(2276..3181)
                     /locus_tag="IWM23_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAP9884945.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3919)
                     /locus_tag="IWM23_002339"
     CDS             complement(3266..3919)
                     /locus_tag="IWM23_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9884946.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVRV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="IWM23_002340"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="IWM23_002340"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAP9884947.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="IWM23_002341"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="IWM23_002341"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAP9884948.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="IWM23_002342"
     CDS             6168..7253
                     /locus_tag="IWM23_002342"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAP9884949.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="IWM23_002343"
     CDS             7250..8314
                     /locus_tag="IWM23_002343"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAP9884950.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="IWM23_002344"
     CDS             complement(8563..9366)
                     /locus_tag="IWM23_002344"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAP9884951.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWM23_002345"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="IWM23_002345"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HAP9884952.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="IWM23_002346"
     CDS             complement(10244..11251)
                     /locus_tag="IWM23_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9884953.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="IWM23_002347"
     CDS             complement(11258..11902)
                     /locus_tag="IWM23_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAP9884954.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="IWM23_002348"
     CDS             complement(12002..12760)
                     /locus_tag="IWM23_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HAP9884955.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWM23_002349"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="IWM23_002349"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HAP9884956.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
BASE COUNT         3955 a         3005 c         2656 g         4425 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 t
//
LOCUS       DACDXE010000077        13918 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.077, whole genome shotgun sequence.
ACCESSION   DACDXE010000077 DACDXE010000000
VERSION     DACDXE010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.077"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            131..505
                     /locus_tag="IWM23_002350"
     CDS             131..505
                     /locus_tag="IWM23_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9884957.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="IWM23_002351"
     CDS             complement(619..1248)
                     /locus_tag="IWM23_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9884958.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="IWM23_002352"
     tRNA            complement(1382..1454)
                     /locus_tag="IWM23_002352"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="IWM23_002353"
     tRNA            1668..1741
                     /locus_tag="IWM23_002353"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            1696..1845
                     /locus_tag="IWM23_002354"
     CDS             1696..1845
                     /locus_tag="IWM23_002354"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884959.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2338..3666
                     /locus_tag="IWM23_002355"
     CDS             2338..3666
                     /locus_tag="IWM23_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAP9884960.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="IWM23_002356"
     CDS             3868..4701
                     /locus_tag="IWM23_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAP9884961.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="IWM23_002357"
     CDS             4717..5454
                     /locus_tag="IWM23_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAP9884962.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6535)
                     /locus_tag="IWM23_002358"
     CDS             complement(5609..6535)
                     /locus_tag="IWM23_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAP9884963.1"
                     /translation="MKENMLTGGVSPDVANPLLDAEITIPKHVELLVEDIFNLPSPHI
                     MPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIGNKLPIVLTGAMRSS
                     NELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTKTHTTNVATFRTPTL
                     GPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGDLLEAIAHTKVDGLV
                     IEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDYEGGGKELEKMGIIF
                     CNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IWM23_002359"
     CDS             6810..7643
                     /locus_tag="IWM23_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAP9884964.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IWM23_002360"
     CDS             7677..8516
                     /locus_tag="IWM23_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9884965.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IWM23_002361"
     CDS             8542..9102
                     /locus_tag="IWM23_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9884966.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IWM23_002362"
     CDS             9301..11022
                     /locus_tag="IWM23_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAP9884967.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IWM23_002363"
     CDS             11187..12329
                     /locus_tag="IWM23_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAP9884968.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IWM23_002364"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IWM23_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAP9884969.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4036 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DACDXE010000078        13805 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.078, whole genome shotgun sequence.
ACCESSION   DACDXE010000078 DACDXE010000000
VERSION     DACDXE010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13805
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.078"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(98..1465)
                     /gene="nhaC"
                     /locus_tag="IWM23_002365"
     CDS             complement(98..1465)
                     /gene="nhaC"
                     /locus_tag="IWM23_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAP9884970.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1574..2995)
                     /locus_tag="IWM23_002366"
     CDS             complement(1574..2995)
                     /locus_tag="IWM23_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9884971.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3238..5115)
                     /gene="tdc"
                     /locus_tag="IWM23_002367"
     CDS             complement(3238..5115)
                     /gene="tdc"
                     /locus_tag="IWM23_002367"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAP9884972.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5406..6662)
                     /locus_tag="IWM23_002368"
     CDS             complement(5406..6662)
                     /locus_tag="IWM23_002368"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAP9884973.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7242..8543
                     /locus_tag="IWM23_002369"
     CDS             7242..8543
                     /locus_tag="IWM23_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAP9884974.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8612..9211)
                     /locus_tag="IWM23_002370"
     CDS             complement(8612..9211)
                     /locus_tag="IWM23_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9884975.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9465..10346
                     /locus_tag="IWM23_002371"
     CDS             9465..10346
                     /locus_tag="IWM23_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9884976.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10359..11969
                     /locus_tag="IWM23_002372"
     CDS             10359..11969
                     /locus_tag="IWM23_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAP9884977.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12046..12705)
                     /locus_tag="IWM23_002373"
     CDS             complement(12046..12705)
                     /locus_tag="IWM23_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAP9884978.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12988..13653)
                     /locus_tag="IWM23_002374"
     CDS             complement(12988..13653)
                     /locus_tag="IWM23_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884979.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         4035 a         2792 c         2532 g         4446 t
ORIGIN      
        1 aagctgaaat tcacaaaaat ttaaaaagca attttcttga gtttcttctt atttcttgga
       61 gttaaacgct tctgtcccag cctctttttt ccttccttca attttttaca cgtcgacgat
      121 tcaataaaaa accgcctaat attgtaaaga caggtgcaag aatcgagaaa aaggcgaatg
      181 gcaagtaatg caacgcgcta actttcatcg ttcccataat gaatgtgccg ctgactcccc
      241 acggtacgag agaattgacg gtcgcacctg aatccgctag tgttcgtgtc aaataggttt
      301 ttgataagcc aacgtcatca aaagaacgct tgaatgtttc tcctggaaga atgatggaca
      361 gatactgttc tccaacgatc agattgattc cgatacagct caaggcagta aacccgacta
      421 aacgagaagg tcggtccatc ttttctttta attcttgaac gatggtatca acgatcagat
      481 atttgatcag taacccacct aatgccaatg caagtaaaat caaactagca gaacctaaca
      541 tactcatgat cccgcctcga gaaagcagtt catctagctt tggatcaggt gtatttgcga
      601 cataaccatt catcaagatg tctgctgctt ttccgaccgt caaacttttc tcatggataa
      661 aagaaagaac gagagcagct gtcgatcccg ctagtaatgt agggatagcc ggaatttttt
      721 tgattgcccc cccaaataaa ataactagtg gaatcaatgt ccaaggagag atccaaaagc
      781 tcgctttcat gttttggatc atttcattga tactagctag atcggctgaa tgactgttat
      841 gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc tgggaaatcc gtataacgca
      901 taccagcgat atgttcgaat agattaactc ctccaatacc cgctgccaag ttggtcgtat
      961 ctgataaagg cgagcagtta ttccctaaaa aggcaccgga aacgactgcg cctgtagtca
     1021 ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa agcaattcca atcgtcgaaa
     1081 tcgttgtaaa tgaactgccg acagtcactc cgacaattgc acaaacaaga aatgctgtcg
     1141 gtaaaaagaa acgcacagaa acaagtttaa atccataaac cataatcgta gggatggttc
     1201 cagataaaat ccatgaagca actaagacac caatcaaaag aaaaatcagg attggaatga
     1261 tccctggagt gattcctttg atgatcccgt catggatctc atcccatgtg aactttttaa
     1321 atctgccata aaacaacaaa cacatgaaag ctgcaaggat cgggatatgt ggcgagagct
     1381 ggaatccgat gatcagtact cctaggagtg ccaacaacac gatcaataca gcgatacttt
     1441 cttttaaaga aatattttta ttcatcgtcg atctcctatt tcaaatgaaa aggccctgcc
     1501 atgaaaaaac tattcacttg gcaggacctc ctactaaatc agcgacaaaa cgtcggctga
     1561 aagacttatt tttttatttt tcttctaaac ttttttctac ctcaatcccc caagaatctt
     1621 tcttgaagtg attgatgatc aatggaataa cgaacatgac cactgtgata gctgctacta
     1681 ataatgcatc actcaatgtc gctgtaccta atgtagcaaa tacgacaacg atgatagaga
     1741 agatcagcat gcagctgact acccaagcta atccgtttcc gtttttacca attcggtaag
     1801 gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa tgaaacagca atcaaaatgt
     1861 acacgataca ataaataaca gttgtagcat ttgtaagtgt caagaataca gcacttacgt
     1921 catccattac accataaagc aaagcaaata atgaaataac gattgattgt gttagaacga
     1981 catttctaga tacgccgtat ttgttttctt tatggaatcc gaattttggt ggaaggaatc
     2041 cttcacgagc tacttggatg attgtttttg atggtccagt aacccaagct gataattgta
     2101 aaagcacacc aataaatacc atgaagctga agatatttcc gatcacttct ggtaagccta
     2161 ggatttgaca gtcaatcaag ataggctgcg tgatatttgc taattccatt tttccatctg
     2221 gtacgacatt tgctacaagc atcgcattga tgacattcaa taaaactaat ccgatcaaag
     2281 cgataaatac gccttttgta taattttttg ttgcgtcttt caaacgaggc atgtaaacag
     2341 aactcatttc gatcccaaca aaaataaatg tgataccagc taagtatttc aatgaatcaa
     2401 tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc acctagatag ccgtttgaga
     2461 ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa catgatgact actgggatat
     2521 atacccctag ccatacaccg atgttcccgc cgacttttgc catatcgaat ttcaagttca
     2581 agatcgtgat gatccagtag ataactaaaa tacaacctaa aacgaaccag tgattattgc
     2641 caagttctac attaccaaat gtgtttccta acaatggtcc taaagtagaa gcgaccatta
     2701 ccatacctgg gaacatttgc acccataata gccatgcaac gacaaatccc catttgctgc
     2761 ccagtgctgt ttttacccat aactgaggac cgccttcttg aggaagcatc gtggaaagct
     2821 caccggaaac cattgatatc ggaccggcaa aaaacaaaac tgcaaaaatt gaataaaaaa
     2881 tcaatgtcca accgactgcg gctaacgtag gaatactccg aaccgttgca cacagtgcca
     2941 ttgtcagacc gataaaggtc ccaagaccca ttttacttac ttcagattta ctcaccgttg
     3001 acactctcct tgattttttg gataaaataa cggtgtcttt gaagtagttt cagcaataaa
     3061 cgaaacaatt cagcgaatat ttgaggggat tctttgagtt tattgcttga agggtttctt
     3121 caaagacaac ctcaaaacaa agggatcgaa gagcttaggt ctccccttgc tcttcagatc
     3181 caaacatgcg tcagaaacag ttagtattca agctgttttc taacatgatt accttgctta
     3241 ttttgcttcg cttgccaata attggtctgc gtagattttt tccaattttt cttgaagagc
     3301 tgctttgatt ttttcagcat attcttcaaa gtgttcttct ttgttcatgt atggtgtcat
     3361 tacgctggca cgcaatacag ttacttttcc ggcacggttc cattcttcat ctgagaatcc
     3421 tagttggttc acaaattgta atgggctgtt gccatagtct gggatagcaa agtcagtatg
     3481 tgaagttaag aactcattac cgtaaatgct tcctttcacg tatgaagaat aatcatatac
     3541 gtcatggttc aatttattca ttgcaactaa gtcatcgttg cctttttctt tgaatacata
     3601 gtctaccata ttgaaatctg gataagttaa tggatgaact tcgatttctt tgtctcctac
     3661 tttaaatgac aagtcattca agaagttgta gaaacgatgt gcaccttcga ttgatgcacc
     3721 cattaattta ccatatcctg tgacattcaa aggtaagaca tggtgtgctg cccaaacgct
     3781 ggctgctgtt gcccctgctt ttgaaccttc caagatataa gccccaagta atgctgggat
     3841 atctgcgcct ttttcaaata catatgtcgc aaagtaagag ataacgtctc tcatgcggat
     3901 atctttgatg acgataccac cggcagaata tggaacatag cccattttat gtgggtcaat
     3961 cgttacagat tctgcttctt cgattgcttt atacgcactg tgtacttctt ctaagatata
     4021 atctttgttt tctgtaaaga cattgtattt gtaatgaaca tcttttaaat cttcaaatgg
     4081 aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg tatccgccat aagcagcatc
     4141 tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg cgacgcaatg caacgatttt
     4201 atcgataccg tcgattgctc cttcttctgt tgatcctaca acaccgacta cacctaggat
     4261 tggtgttttt tcagctgcta gaccacgaac gattttttca agttcgttga tgtccatgcg
     4321 atagttgtgg tcaactggca ctggaatcac ttggtctaaa ccgataccga tgatgtcggc
     4381 agcttttagc catgaatagt gtttagtttg tggaaccaac cattttccta atttttctaa
     4441 atgttttccg ctacgtgctg agtgagcttt gatttcgtcg attttttctg ggacgctgtc
     4501 taacagattc atgatttctt ctgttgacaa gttcattaat tcccaatcgc tttttccagc
     4561 aactagttca ggtgtcactt ctttcattgc aagcggcaat gatttgatgt tgcgggcata
     4621 ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg atgtggcccc agccatcttt
     4681 atagctcatt aattttgcaa attccattcc tacttcttct tccatttggc ttgttgcagg
     4741 agaagattca taggcaacgt tattaccatt ccatagcata gcaaaattgt acgctagtaa
     4801 agatggcatc aatgtttcag agttcatatg accccagtaa cgtccggcat tatgccaagg
     4861 aacggaatgg gtacgcatcc gcgctgaaat ttctgataga acatcctttg ttttattcac
     4921 agtgtgttca aaactagcac tgcttttttc ttctggcgta ataataggca tgtcttgagg
     4981 catgtagttt tgacgccagc ctaaatgttc atccaccaat tcattcaaca aagctttata
     5041 gatttggccg ttctcagctt tgtccccaat gaatagggca tttaggttta gatctttcga
     5101 caatgattca ctcatagtga aaccctccta aaaaaatatt taaattttgt cgagccctac
     5161 attacacctg ttcttctatt ctgtaaacta agttttggcc tgatttttaa tcatatttta
     5221 atatgtatca attatccgaa ttgttcacac ttttgaaata attaaaaaaa acgagggaat
     5281 ttacaaatgt aaactccctc gcaaagattg tattaacgac aaccagcgtt ctattttagg
     5341 atgggtttca aaattggaat gttgtaatag tatttccctt tttcaaggta aaaacttact
     5401 aaattctaat caattacttt ggccaaaaag taatttttct taccttttcg aataaccaca
     5461 aacttaccat cgaattcatc ggatggattt atgacaaaat ttaaatcggt aacgcggtca
     5521 ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg cttgacgttt agatggttca
     5581 atttttgtgg aaacaagcag ttccacgata ttttcaggtg tgcttgaaat ttcaacgttc
     5641 ggcattttac cgaatccttg cgcgatttct tccgcattta gttctttaat atttccagag
     5701 aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt cttcactatg cacaaatctt
     5761 gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct caggttctgt ctctactttt
     5821 ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa agaatttcaa gtatttcact
     5881 acatcgcgat catcttgatt caaccagaat tggtagaact caaatggtga agttttcttc
     5941 ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt tcgttccgtc agccttcagc
     6001 atcaatggga tcgttaaacc gaacgcttct gcttctggtc cttctaattt tcggattagg
     6061 tctaatccag ctgtgatatt tccccattgg tctgctccac cgatttgcaa ttgcacatcg
     6121 tatgttttat gcagatgcaa aaagtcgata gattgcagga tttggtaagt aaactctgta
     6181 aatgagattc ctacttccaa acggctggca acgatatctt ttgctaacat cgtgttgatg
     6241 ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag agatttttga caaccagtcg
     6301 tagttgttca caaatgtgat atttgcatct ttaccaaaca attttctcat ttgattggaa
     6361 agtgcgtcca cgttatgttg cacttgttcc atcgtttgca acacacgttc tgttttgcgg
     6421 ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca gaatatatgg atgatggcct
     6481 gccaattgga atcttttcat catcataaat ggaatcaaat gtccgatatg catcgaatca
     6541 ccagttggat caacgccgca ataaagtgaa atgctctttt cttcaacgag ctcgcgaagt
     6601 ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag ttaattcatc aataatattc
     6661 attttactga cctccgtgta gtttttttat gaaagcagga acctgctagc atgtgtaatt
     6721 aattatttct tcaggatagg ataggaaata aaaaaagtcc cttggcatat aagccaaggg
     6781 acgaaatctc gcggtaccac ccaatttgta tgtctagata tccaacatac ctctcgaaca
     6841 tttaacgctg tcgcgtggca atcactgcca aagactcctg aaatgtaatt cgctttaaag
     6901 catgtactgg tttgcagcga ccaccagctt tctgaaacat tggctttaac aactacttcg
     6961 tttcgatctt cgtcttgtta ttattaatat gtgtctatca tagagcgcaa ctttttcttt
     7021 gtcaacagaa aaaggatatt ttttttaaat tcctcatttt ttcttttttg aatccgctct
     7081 aattccgaac attttatttt caattattgt taaaagaaat aactttttct gtattttttc
     7141 tgcaatttat cgagaagtca ctttactttg cctcttattc tttcttcgtt acaataagga
     7201 atgggacaag taaaaaaatg aaaaagcaag ggacgaggta tatggaaaaa aagcaaaaaa
     7261 ttattttagg agccacttct gctttaacta tcgttatttt aattctaagt ttgttttttg
     7321 tcatccgatc atctacaaaa aaagaaccaa tcattcctga aaaaaaaata acagaaaaag
     7381 caaaagaacc gacagatgat aagcaacaac tagttactca agcaaaaaat ctggctcaaa
     7441 gctacgatta tgaccaagcg attgcgttat tggaaaaaga tagtaagaac ggatctactg
     7501 aaacacaaca gcttcttcaa acactaaaaa aggagaaaga acaactgatc gactggtctg
     7561 atcctactca gatctctcat gtctttttcc acagcttgat cgtggacccg caaaaagcct
     7621 tccactctca acaggcacaa ggatacaaag attatatggt gactgttgaa gaattcaacc
     7681 gttcgatcga acaactttat caaaatgatt ttgttttagt taatttgaat gatttgatcc
     7741 aaaaagacga acaaggaaat ctttccttta acgggcttgc gctacctgaa ggaaaaaaac
     7801 cattggttct ttcacaagat gatgtcagct attatgaata tatggataat tccggttttc
     7861 ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa tgtctataaa gagaaagata
     7921 aagaagaaat cggtgacttt gacatggttc ctctgatcga tgcctttgtc aagaaacatc
     7981 ctgatttttc ttatcgaggc gcaaaaggta ccttggccct gacaggttac aacggagttt
     8041 taggttatcg aacatcaaaa tctcaatatg gagacaatga aaaaacaaaa aaagagatta
     8101 aagaagctaa aaaagtggcg gaacagttga agaaagacgg ctggagcttt gcttcccata
     8161 catggggtca cttgaatatg actcaagctt cactagaaga tgtaaaaaaa gacaatgaat
     8221 tgtggcagca agaagttgca cctatcctag gaaaaacaaa tattctgatt tatccatttg
     8281 gtgcagacat cagcgactgg cagccttatt cagcagaaaa tcaaaaattt gcttacttga
     8341 agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc tacaccagct tggggacaat
     8401 taggtacgga tttctatcga aatgctcgga tcaatatcga tggcattcgt tttcaatctg
     8461 atttagacgg aaaaaatcct attcttgatc cattcatcaa tgtccatgat gtctatgaca
     8521 aagaagcacg cgaatctgct tgattttacg tcatttatga accaaaaaag ccgaactgac
     8581 attttatcgt cagtttcggc ttttttatgc tttatttctt cttcggtgcg attcccttga
     8641 tcaggacttc tgtccattct gctgtatatg gaagcagttt tgcgtatatc tccggcttag
     8701 aaattccttc taccattgct tgaaaataga ggatcaagaa ctcatccgaa tatttcaaat
     8761 ccacttttcc ttctttccgc cctcgatgga aaagatcaag catgatactg aaactttctt
     8821 gcgtatacgc atccatcgcg tcagaaaatt cttttttttc tttttttgta tagtatgtca
     8881 tcaaatcgat ataaaattgt ttgttcactc gattgagata acggattttg ttttcactca
     8941 tggcaatcag tgtttcttca aatggaagat tttccgcaat caacgctttc gcttcttgcc
     9001 ctaaatcatt cataaatgca aaaaatactt cataaatcag cccttccttg ctgccaaaat
     9061 atttaaaaat cgtcgcttta cttacctgag cgttctcagc taccttatcc attgtcactc
     9121 cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc ttgtaaaatc tctttctttt
     9181 tctcttgtgc tctcttttca aaaccgttca tttgtagagc ctcccgtaat tttaaacttt
     9241 tataatatat atagttcaaa tttattctga ggtttattat aactatctat tgagaaagta
     9301 tcaagctagc aattccttgt ttatgaattc attgacagaa atttataaaa agaatattat
     9361 gtactaaaat aataaaaaaa gttttataaa aaggtataaa acctttttat taaactattt
     9421 aatcttgttt agtatatatt tttagaaaaa ggagtggggt ttttatggca gaaatcgtca
     9481 aagtcacaca tttgaaaaag agctttggtc gtttcgaagc tttaaaagat gtctctttca
     9541 ccgtacattc aggtgaagtc gttggattta tcggaccaaa tggttctgga aaatccacga
     9601 cgattcggac acttttaggg attataaaaa aagacggcgg aacggcaacg atttttgggc
     9661 aagatgtatg ggaagatagt ttatctatcc atgaacgtct ttcttatgtt ccaggcgata
     9721 tctctttgtg gggaaatttg actggaggag cgatcatcga tctcttcatg aagcttcatg
     9781 gaaaagggag tgctcaaaaa aaagatgaac tgattcaacg tttctctctt gatcctaaga
     9841 aaaaagcaaa aaactattca aaaggaaatc gccaaaaagt gggcttgatt gctgctttgg
     9901 ctgttgattc ggatctctat ttatttgatg agcctacttc tggtttagat cctttgatgg
     9961 aaacgatttt ccaagaagaa gttgaaaaat tgaaagaaca gggcaaatcc atcctacttt
    10021 cttctcatat tctcagtgaa gtcgagcgac tagcggataa agtcgtgatc atacgcgaag
    10081 gaacactcga tgaagaagga acactcgatg aattgcgtca tttgactcgt tcgaccatca
    10141 ctatagaaac agaagcagag aatagtcact tagcacaatt gaacggtgtc catgactttg
    10201 tgcaaaaagg tcacctcgct caatttgctg ctgataacga agctttcgat gtgattttag
    10261 ctgaagcgtc caaattaggt gtgaaaaaat ttgaatctat tccaccgact cttgaggatt
    10321 tattcatgag acattatgaa ggatgaggag gaagcgaaat gactgataaa ttttcacgct
    10381 ggtccaccct tttttctcaa aaccttagcc gtgactggaa aaagatcctg atatggctag
    10441 tcggtgtcac tgctttttct ggtggttttg tccctgcgtt tgaagaattg acttccggac
    10501 aaggattagc tggattatac gaaaccttgc aaaacccggc aatgattgca atggtcggac
    10561 caactccagc ttcgaatgct gctgagtata ctttaggagc gatgtatgca aatgaaatgt
    10621 tgcttttttg cagtttgttt gctgctgtga tctctgtttt acacgtggtg agccaaacga
    10681 gaaaaaaaga agaacttggt ctgattgaac ttattcgttc ctttcgagtc ggacgtcaag
    10741 caaactcttt agctgtcatc gctgaaagta ttctgatcaa tttgctttta gcaatatcga
    10801 tcagctgtac gatgattttt ttccaagcgg attcgatcac tgcagaaggt gcttggcttt
    10861 ttgggctatc tgtaggtagt atggggattt tggggagtgc cgttgcactg tttgctgcac
    10921 aactgatgcc aagttcagcc agttcgaatg gtctgtcttt agcgttttta ggtggacttt
    10981 accttctacg agcaataaca gatgtagctg attcctccct ttcgatgtgg aatcctttag
    11041 gctggtctta tttgacctat ccatttacaa ctaacaactg gattcctttg ctttatttgt
    11101 ttttgttcag tctaatcttg ataatcagtt catttgtttt agaggaacat cgagatttga
    11161 acaccggcta tatccccgaa cgtgaaggaa aaccgtatgc taaacgttct cttttatcct
    11221 tgcgtggttt attattctat ctaaataaag ggatgacaat cagttggctt atcacttttg
    11281 gtttgatggg ggcggcatac ggctctatct acgggaatat gcaacagttt ttagaaagta
    11341 acgacttaat gaaacagatg tttatccaat ctggcgtctc aattgaaaaa aactttacaa
    11401 gcacgatcat ggtcgtcttg atttgtctag ctgctatttt accaattgct agcatcaatc
    11461 gtttatttac agaagaaacc cacggacgta tgaatcagct gttcgcaaca aaaatttcta
    11521 ggaaccaact ttattggacc aatatgttac tagctattct atttgctata ttggcaatcc
    11581 tgattagttc tggtgcacta gggctttctg ctttggcttc gatgaaggat gcgaaagaca
    11641 tggatctatt gatgtttctt aaagccgggt tcaatttcct gcctgccgtt ttagtgttta
    11701 tcggaattag tggctttcta ttcggatggc ttcctcgaat cggtaaactt gcttatatct
    11761 acttagctta ctcctttgca ctcaattact ttggcggaat tttggattta ccagaatggt
    11821 tttccaaaac agctgtccct agctgggttc cacggatgcc aaacgattct tttgatcttt
    11881 ccgtgttcct tacgttatca tgtatcagcc ttgcactgtt cgtccttggt tctattggtt
    11941 ataggcgaag agacttacta gaaggataat aaaaaagcat gtacaatttc atgctagcct
    12001 ccaaatctgg gtcaaaaatc caacatttgg aggtcatttt tttatttaca agtcttctaa
    12061 ctgctcttta tcaatgatcc agacttctcg attctttctt tttacataac cattctcctc
    12121 taataattta aattttctag ataaagtttc gggggtcgta ccgataaatg cagcaagttc
    12181 tttcattttc attggcagga aaaagtgatc cttccctgtc gctttagcta agtcaagcag
    12241 atagacagcg actcgttctt ctactttttc catcatcaaa aaagttgtct gctgctgtac
    12301 ttttgttaat ttctcaacgt tgattgctaa aagtttcaga ctaagggcgg gataatgcag
    12361 taagagttcc gagaaatctt tttgtctcaa tatgcaaatt tccgtttttt ctagtgcttc
    12421 gccaaataaa gactcatttg gaacgcctag caactggctc tctccctcat atccgcccgg
    12481 ctctattact cgtaaaagct gttcctttcc gctagcagat aattgataca ctttcatact
    12541 gccggaggcc acgatcacta attgaggttc tccattcggc gcaagaatct gttctccttt
    12601 ttccacatag tgatgtttga caagtgcgtg tactttttct tgatcttgct gtactaagtg
    12661 attgaacaaa ggaacaaatg aaacacataa atgatcatga ctcataatct ctcctccttc
    12721 taaacagcta tttctaataa agaaacataa cttcccttat tccaactgat aattttcttt
    12781 tatcatagca taaacgtcaa gcgaaccaga taccagcaaa tagagtggat ttctttttgt
    12841 cccttgcttt attttccttc ttttgagacc ttaagtaatt gattcctatc aattttttaa
    12901 atatttactt tttgaataat caaaaacaaa ccttcactcg caagccgaga gtaaggtttg
    12961 tttttcgcat tgcttatttc gtgttgtcta ttgaaatttt tccgcaccta gcttttgata
    13021 tagtggtgct agttcttgta aggtttcagc ggttatttgc tcaacgtgtt cgatttcttg
    13081 cggatagaag aatagtttac ccatttgaaa ggttcggtct ctcatcgctt ttttcaactc
    13141 ttcttgtttc tttgtatcgg acaaatagtc agacaaagct aagtggtcgt caagttcatg
    13201 ttctggttgc ggccatatgt aataatcctt gatatcgact gttttgctcc actctttcag
    13261 tatcgcaagc aactggttat acgctgcaat gctaccatat cgttcttcgc ttttataatg
    13321 ttggtacatc aaatagattt ggtattgttt ttgattcaac aaaactgcta gacaggcatt
    13381 ctcgtcttga cgagattcgc ttcggtacgc gctccaaaaa tgactgcgta aattccagcc
    13441 attcgtccag ctttcgattt taggtttgct catgccgtac atatcaggca aaagtgcagc
    13501 tgtctggagc tgaatctctt tccatttttg ccagtgttct ttatattctt tcttgatttg
    13561 ttcaatctta tctgttggat ggctctcttt gagctgtttg aatgcgtaga ttggtcgatc
    13621 aaacaactga taatctgatt ttccaagttc cattgtttcg cctctttccg ttatcttttg
    13681 tattctacct gaattttgcg atatggacaa atagaggttg tgaaaaagct gttctgcatc
    13741 aagtcataag aacagccaaa caaaaaactg cgatgagcat caccgcagtt ttattggctc
    13801 tataa
//
LOCUS       DACDXE010000079        13782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.079, whole genome shotgun sequence.
ACCESSION   DACDXE010000079 DACDXE010000000
VERSION     DACDXE010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.079"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(143..1345)
                     /locus_tag="IWM23_002375"
     CDS             complement(143..1345)
                     /locus_tag="IWM23_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9884980.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1348..1542)
                     /locus_tag="IWM23_002376"
     CDS             complement(1348..1542)
                     /locus_tag="IWM23_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9884981.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1589..2437)
                     /locus_tag="IWM23_002377"
     CDS             complement(1589..2437)
                     /locus_tag="IWM23_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HAP9884982.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2642..2992)
                     /locus_tag="IWM23_002378"
     CDS             complement(2642..2992)
                     /locus_tag="IWM23_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9884983.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3587..4033
                     /locus_tag="IWM23_002379"
     CDS             3587..4033
                     /locus_tag="IWM23_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAP9884984.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4657..5649
                     /locus_tag="IWM23_002380"
     CDS             4657..5649
                     /locus_tag="IWM23_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9884985.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5665..6549
                     /locus_tag="IWM23_002381"
     CDS             5665..6549
                     /locus_tag="IWM23_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018165023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9884986.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6598..8205
                     /locus_tag="IWM23_002382"
     CDS             6598..8205
                     /locus_tag="IWM23_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAP9884987.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8206..9690
                     /locus_tag="IWM23_002383"
     CDS             8206..9690
                     /locus_tag="IWM23_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAP9884988.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9867..10859
                     /locus_tag="IWM23_002384"
     CDS             9867..10859
                     /locus_tag="IWM23_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9884989.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10911..11780
                     /locus_tag="IWM23_002385"
     CDS             10911..11780
                     /locus_tag="IWM23_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9884990.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWM23_002386"
     CDS             complement(12200..13765)
                     /gene="guaA"
                     /locus_tag="IWM23_002386"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAP9884991.1"
                     /translation="MSSPTRFDEEPKIIVLDYGSQYNQLITRRIREFGVFSELMSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDEKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGK
                     VKPAKNREYGKAELEITSAEAALFQNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCP
                     IASIQNAERKMYGIQFHAEVRHSEYGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGK
                     FGLNIIKVDAKERFLSKLAGVSDPEEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGELTEEKLEIVRESDAILREEIANAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
BASE COUNT         4230 a         2329 c         2843 g         4380 t
ORIGIN      
        1 ttgaaaaacg ttgatttatc ggcattcccc atccatatta tatttacgtc ttattcccac
       61 tcaatatgag tagcctcgta aagaagctat tatatcagtg ctttttcagc ctattcctca
      121 ttttggtagg gaaatggtag ggttagattt ttactgcttg atttccatta aagtttgcaa
      181 ctttagattt tgttgcacag gtaaatttaa tgtttcctgt catttgttct gcaattcctt
      241 catcattacg gctccataga tgagcgtagt gctttaaagt gatttcaggg ctagaatggc
      301 ctaaccgttg cgacacgacc agaatgtctg cgttaaactc attgattagg tagctaacat
      361 gagaatgacg taatcctttt ccttgtatta ggggtacctt agctagtttg gcataacgct
      421 gaataattcg ttgaacagtt gaacgataga gtgggaggtc agtgtagcta agaataaatt
      481 gttctacacc atgatttttt tgaataactt tccacttttt tagcacttca attgtatcat
      541 cgtccagaga aatgatccgc aagccgcttt cagtttttgt gtaaggtttc cgaataaaat
      601 ctttttgatt tgtcatttcc aaggtgtgat gaaccctcaa ctttttacgt ttgaagtcca
      661 cgtcattcca attaagtgct aatccttcgc ttacacgaac accagtcatg taatatagcc
      721 agatcattaa aaaacacata tgttcgtaga aatcatcttt ataaattttt gccaaaactt
      781 cttcaaattc ttcttttgtc cagtaaggaa cgattgattt tccttttgga attgcttttg
      841 ttcttttgga aatattctca ttcagaaatt gcagggtgac cgcatagtct aatgatttac
      901 gaaacatacc gtacataagt gaactgtatg cttgtgagta tccgctatcg ttcaataacc
      961 aaattcgata attttcacaa tctcgcacat tgatttcacg aagcttctta tcaccgaaaa
     1021 attcagtaat ctgaatcaat ccacactcac gagaactcca agtacttttt tgtacgctag
     1081 ctttgtagtg tggaacgtaa gactgctcca taaattcttt gtaagttaaa tcataattcg
     1141 caaatccatt tacaaccatg tattcatttt ttatacgcat gacttctttg tgtgcttcac
     1201 gagctgatga gaatcgtttt cctagagaat cttttgttcc tttcttctgg atcctctttc
     1261 cagtcacttg atctgttcct aactcaacta gataagaaaa gcgaccattt ttatcttgat
     1321 acacacaagg gtattttgtt ttcatagtta ttcctcctgt gaaagaggaa cacctataat
     1381 ttcttcaatt acttgtcggg gtactacccc aactctttta ttattataat atggatatcc
     1441 ctgatttacc attcttgttt ttgcttgtcg gattattgtg gtagcttgat gtgttttaaa
     1501 tcctagagcc tctaaatctt ttcgacaaat tgtatttttc attgtataaa ctcactttca
     1561 aagaaaacac tatcattggg taatagtgtt atttctttat ttttgtataa tgaacggtac
     1621 tttcttgaaa atccatcact tgttcaggac ttcccaactc atttaaagct aaagcattgt
     1681 aaaaaaatgg acgtctaact atatattcgc ttgtatatag tatatcttga ttcgtggaat
     1741 catagggttt ctttaccatc tttttggtga cagttggtag tttgagattc cgggacttga
     1801 aaaaagcttt tttcttatgc tctttcaatt ccagctcttt atcgaaatat ttgctgatat
     1861 agcgcccgcg attttcgata ctgtctacat cgatcttgtt gatcttaacg aaaccctgac
     1921 cccaaatcgt agttaaccgg ttaaatgaga taaaggggaa atcaaacaag ataatatggt
     1981 agtggattgc accgcgttgc tgcttttccc acgtggcgag atactttaaa tttgcttttt
     2041 tagttttata gaggtcataa ttcagtcgtt tgataaattt tttaaattca ttatttgcat
     2101 actcaatgtc ttgaatgttt tctttgaaag tgagagttaa aaatttcgtt tgattgtcaa
     2161 aattcgtgtc tacaaggcgg gcaatctcaa atcgcatact ttgataatgt ttctgtttgc
     2221 gttttaaact gtcatattgt ccttgagcgg ataattcatc atagtttttt cttttttcaa
     2281 gagtaatatc attttcataa atccaagcta atttttgttt tcgttccaga tttacttcgt
     2341 tttcgatcgt tttacgtttg ggagtaatga ttgcttcata atcataaatt tcaatatacg
     2401 tgggagtttc aatgattttc tgattataag cttgcaaaaa tcatcactcc ttgttgcgta
     2461 ttcgattgtc aaagaaccag tcattttctc ctgatgttga tctataaatc aagttaaggg
     2521 ggaaggcctg cgtagcagcc cttctagcct atggggtgtt gggcggtctt aacggacaga
     2581 aacggctctg tgggcgctgc cccgccgttt cgtcctaacc acccaacact ccatagtata
     2641 gttagttttg tgtagtggta ccttgttttc tgggaatcaa agttgccgta tcagcggttg
     2701 ccgataaccc ataactgaca aattgtccat tcgagcgtcc ccaaggtaca atcaccaaat
     2761 tggtaaatgt aattatggct aaaccttgtt gggtaagttc ttccggcgtg atcacaggat
     2821 gttcagattt gactttgaca ctgatttttt cgtagcgaag ttttggtagg atacattcgt
     2881 agctccaacc tagaatttcg ccctcctttt gccagcgagt gacattgacg acttggtatt
     2941 ctttttcgag tgtttctttt ggtaaactga gatcggtaat ttttattggc atcacaattc
     3001 tcctttttta tacgaaagtt tatacgattt gattaaaaaa tatagatggc catctacgac
     3061 ttgtattata tacgagaact tatacgtagt caagagattt tctaaaaaaa tatatacgat
     3121 tttttatacg agtgttataa tgaaaaaaat gataaggagg aatttgaata tgtttggttt
     3181 agtacaacct aataaagtga aggtgggcca acgaatcaaa gaaataaaag acggaatgtc
     3241 tctctctttt acagaattag ggaatcgatt aggtttaaaa aagccgacga tcagttccta
     3301 cgttcaaggc tatgcgttgg ctcctaagag tgtgatcaac caattatcta gtatcagcgg
     3361 aaaacctgtc ggctggtttt attttgggca tgtggaagaa tatatccgag agtatttgtt
     3421 tctcattggt cagaaaaaaa tagtggacga ttatcctgaa atcattgaga aaataaagca
     3481 agaattttat acgggcgagt tcaaaaatcc tgcttgggag aatgagctag gctatccttt
     3541 ggaggaattt atccaagaat gttttgtaga atattcgagt gaaatcatga aggactatgt
     3601 cgtacagatt acgaaagagg tacttgaaga atccaaaacg ctaaacaaac tatcatcaaa
     3661 agcaaaagaa gaagctgtca cgattctttc atctgggatc attgactata tcgagatgag
     3721 tggtgagatc aagtatggag agaaagatcg cattgcgaaa atggtgaaag aatcaataaa
     3781 aacctttgat gtgaaacaag acctgacatt taatgattcc tatttgattg gaaagttgat
     3841 caatattctt ggtacagatg aagatacgga gctgtttctt tcttctttat caatgatatt
     3901 tactgataag catttttcca ctcgatttgg tggggaagaa ttagtcgaaa tatttcaatc
     3961 gatgcgacca gcgttaatca aactctatcg ggagaaaaca ccagatgaat tttatgattg
     4021 gtttgagaag taaaataaga aaagtggctt agattacaag ttggtgaaaa aatttttttc
     4081 agcataacat cagtaatcta taaaggggtt gttgattata ctgtagaagt taagtactca
     4141 agagattttt tgtacgtaaa caaagcaaaa aagggtgcag tttcactaga aaatgtactc
     4201 ttttttgtga tttttcttag ttatagagtt tttttgaaac tttaaaaatg agaaaacata
     4261 ttgattttaa agagtgtaga catagcttct tttgctaaat agattaaact tactggtgag
     4321 tggtcttgaa catactttta aatcacattt tactgagttt tattggattt tataattcat
     4381 tggaaacctc gtacttaatc tgtttttatc tatcgactag aatctgtata tattccagct
     4441 atcaatgagg ccgcagaaaa gggtgcttaa aaataatctg ttgtttaaaa tattattatc
     4501 agaatactta gatacaaaat tctggcacaa agtaaaaaaa atattgactt aaaaaaatgg
     4561 atatggtaat ctataaacga aaatagtggt aacgtttcca aaatgcatct tcgttaaatt
     4621 tttatttttt taaacgaaaa tggaaacgtt tctaaaatgt attgggagga gattgaattg
     4681 gaaaagacat tgggaccagg tggtgagata agggcggtac aacgggataa aaaaaatatt
     4741 tcgaagtggg aaaaattaaa gaaacagttg cggcgtgata agtggatgta cttgttgctc
     4801 ttaccaggta tcatcatgac gtttatcttt aagtatgtgc caatgtacgg tgctgtgatt
     4861 gcatttaaag actataatcc tatgctagga attttggaaa gtccttgggt aggactagat
     4921 cactttaggg aatttatcag ttcaccaaat tttacgcttt tgctgagcaa cacattaaaa
     4981 attagtattt atggtttatt gttaggtttt tttccaccaa ttattttagc actgagcttg
     5041 aatcaaatta tcaataaaaa aataaaacag aaaatccagt tgattctata tgcgccgaat
     5101 tttatctcag tagttattat tgtggggatg ctgtttttgt tcctatccgc aaatggacct
     5161 attaatgggt tagtagaaaa ggttacgggg caaccaatta tgttcatgtc taatccagct
     5221 tatttcagaa cgatttatat tttatcggga atttggcaag gaatcggctg gtcatccatt
     5281 ctttacaccg caacgctggc gggagtcagt accgatttat atgaggcagc aaatatcgat
     5341 ggggcaaata ttttacaaaa gatttggcat atcgatttgc ctgcattgag gcctgtgatg
     5401 gtgatcagct tcatcttggc cgccggcggt attatgaatg taggatatga aaaagcctac
     5461 ttgatgcaaa cttcaatgaa catccctgcc tcagaaatca tttccactta tgtatacaaa
     5521 gtcggcttgc aatcaggtga ttatgcctac tctgctgctg ttggtctgtt caatatgatt
     5581 attaatgtcg ttttattact gattgtcaac aatataacca agcggctaaa cgatggtcaa
     5641 ggcttgtaat gaaaggagaa aattatgaat agtgctttat caaaaatcga ccgtcaaatc
     5701 ttatggttga atcgagtgat tgtaactttt ttagtcgtca tcaccattgt tccgctgatt
     5761 tatgtactgg tagcttcttt tatggaccca gctactttga tcaataaagg tataagcttc
     5821 aatccgaaag attggacaat acagggatat caacgggtat ttgcagatga ttccatttta
     5881 cgcggttttg taaattcgtt gttttactct tttagcttca gcttattgac tgtatttatt
     5941 acgatgatca ctgcgtatcc tttgtctcga cctaacttgg cggggaaaaa gattattatg
     6001 actttttttg tcattacaat gttcgtgggt ggtggcttga tacctaccta tttattggta
     6061 aagaacttgg gcatgttaga tagtgtatgg gcgattatcg taccaggtgc tataaatgtg
     6121 tggaatatta tcttagcccg tacctatttt agtgctttgc caaaagaact ttctgaagct
     6181 gctactatcg atggcgccaa tgacttgcaa atactgttca aagtcatgct tccattagcg
     6241 aaaccaatta tgttcgtatt gtttttatat gcctttgttg ggcaatggaa ctcatacttt
     6301 gatgccatga tttacatcaa agatcccgac ttacagccgt tgcaactcgt attaagaaat
     6361 attttgatcc aaaaccaacc ttcacaagac atggtgggag ccaatactgc catggcagaa
     6421 atgaaacaaa ttgctgagtt gatcaaatac gcaacgatcg tcatttctag tctgccatta
     6481 atcataatgt atcctttctt ccaaaaatat tttgataaag gtgttttagt tggttctgtt
     6541 aaagggtaag tattgttcac tatttgtatg aataaaaatt ataaaggaga ttttaagatg
     6601 aaagcaaaat ggatgctgcc aatcgttttt acttccgtac taattttggg ggcttgtgga
     6661 ggctcaaaag aaaagagtaa atcaagccct gattatgaac tagaaaatgt ctcttttcca
     6721 ttgaaagaaa aagtatcttt aaaaatgatg tctcaaagtg cccctttggc tcccaatgat
     6781 cctaatgaca aattgatttt ccaacgatta gaagaagaga ccaatctcca tattgattgg
     6841 acaaactaca gtagtgattt tgccgaaaaa agaaatttgg atatcgctag tggtgattta
     6901 ccagatgcca tcttcaacgc cgctgcaggt gactatgatt tattaaactg ggcggaaagt
     6961 ggcgttattg taccgattga agatttgatc gaagattata tgccaaatct gaagaaaatt
     7021 tttgatgaaa accccgaata ccgacaaatg tctactgcac ctgatggtca catttactct
     7081 atgccttgga ttgaagaatt aggcgaagga aaagagtcga ttcatacagt aaatgggatg
     7141 gcttggatca acaaggagtg gttggataat ttgggtcttg aaatgccgca aaccactgat
     7201 cagttgatga ctgtgttgga ggctttcaag acgcaagatc caaatggtaa tggtgaagca
     7261 gatgaaattc cttttagctt tattaacaat ggaggaaacg aagacttaaa atttttaatg
     7321 ggggcattcg gtctaggtga taatgatgac cacttagtcg ttgataacga tggtcaggta
     7381 caatttactg ctaacaaaga agggtataaa gaagccatca aatatttcaa tgaaatgaat
     7441 aataaaggtc ttattgataa agaggctttc gaacaagatt ggaatacgta tgttgccaaa
     7501 gggaaagaac ataagtatgg cgtgtacttt acttgggata aagcaaatat cacgggtgca
     7561 gatgatagtt atgacgtctt gccagtctta gcgggaccag atggaacgaa acatgttact
     7621 cgtacgaatg gcatgggttt ctcaagagat cgtttcgtaa ttactagtgc gaataaaaac
     7681 ttggaactta cagctaggtg gatagataaa atgtatgaac cgttacagtc tgtccaaaat
     7741 aactggggaa cctacggtga tgaagcacag gcgaatattt ttgaattaaa ggatggtatg
     7801 ttaaaacatt tgcctttaga ggggacagca ccgggggaac taagacaaaa aaccgaagct
     7861 ggaggaccat tagcagttct tgattcttat tacggcacag tcactactat gccagatgat
     7921 gcgaagtggc gtttagattt gatgcatgac acgtatttgc catatattac agaagacaat
     7981 atctatccac gtgtcttttt agaaaaagaa gaaacagatc gattagcaaa aatcgaaacg
     8041 gatatgtggg attacatcta tcgcaaaaga gcagagtgga tcaccggcgg taaaatcgat
     8101 gaagaatggg aagaatacct tgctgaattg gaacgtgtag gtttggaaga atggattcaa
     8161 ataaaacaaa ctggctatga taattttttg aagggagcac aataagtgtt agtagaagaa
     8221 acggcgattt tggataaagc aaatcgattt attgaaatga tgcgaggaaa agttaaccct
     8281 cagtatcgac aaaactatca tttttccgca cccatcgggt ggataaatga ccccaatggg
     8341 tttatatatt acaaagggca ttaccatctc ttctatcaat actatcctta cgatagtcga
     8401 tgggggccca tgcattgggg gcatgcgcgc agtagtgatt tggtccattg ggaagatcta
     8461 ccagtagcgt tagccccaga tcaaccttat gacagcggcg ggtgtttctc aggaagtgca
     8521 attgaaagag acggaaaact atatttattt tatacaggct gtcaagaaat tgatggtatt
     8581 agccatcagc atcaatgttt ggcggtctct gaagatggta ttcattttag caaatacagt
     8641 ggtaatcctg tggtagcaga aaaacagcta ggtgaaaatg ggctaataca agaatttcgt
     8701 gatcctaaag taattgttca caacgatggt tactatatgg tagtagctac taaatctaca
     8761 cagtctactg gtagaatagt gatgtttcaa tcgtcagatt tgtatacttg gcacttttac
     8821 tctgtgctgc tagaagggac ttcagagcaa ggaataatgt gggaatgtcc tgatttattc
     8881 catttagacg gacgagatgt cttaatcatg tctccgatac aaatcaagaa gaacggactg
     8941 gaatatcata atatcagttc taccatggct tgtattggtg aggtaaactg ggaaacaggt
     9001 cagatgatag ttgataattt ccatgaaatg gattttggaa tggattttta tgcaccacaa
     9061 acaacaagag atcctaatgg tggacgggtg atgattgctt ggatgcagat gtgggatcgt
     9121 acattgccta cacatgagtt aggccatctt tgggcaggct ctatgagttt gccccgagag
     9181 ttaagagtga aaaaaaatcg attggtacaa aaacccatct caactgtata tagtcattta
     9241 gtttatcaac aaggcagtga aaatattgca gtgactgaca catcagtcgt atttcgaaac
     9301 gcgctgtctg attcttcata tttacatcta gtcgcagatt tggagaaagc tgaagttttt
     9361 acaattgaat tactgcggtc taagacggat tgtctcaaac tagcctatga cactgctaca
     9421 caggtttttg aattggatcg tcagaaacta ggaagagagc ttacaggggc ggaagaagac
     9481 cctctgaatg tacgaaaagt tgaagtacct ttgattgata atcagctcgt tcttgagatt
     9541 tttagagata cctcatcaat tgaagtcttt attaatgggt tagaagttat gacggctact
     9601 ttctatgagc tggaaaaagc tcgagatatc ttgtttcatt cgatcggaga aagtcgtatt
     9661 caagcttttg aaacagcgag tgtgaaataa atgtttgatt gcttaggcga tgaatcaatt
     9721 atttaatagg ttttgaagga taatgttttt agaaaagaac agatagctag atttcccatg
     9781 gatttgctat tagtattttg aaaaaattag taatttatga caaaattcat tatactaaac
     9841 ttgtgcagaa atgtgaggga tcaactatga aagcaacaat gaaagatgtc gcaaaattag
     9901 caggtgttgg agtaggaact gtttcccggg tattgaataa cggttctgtt aaagcttcta
     9961 ctagagaaaa agttgaagca gcaatgacag agttaaacta tcaaccagat ttttatgccc
    10021 gggggttaaa gatgaagcat acgaacacgg ttgctttgat tctacccacg gtatggcatc
    10081 cattttttgg cgaatttgcc taccatgtag aaacagcatt agaaagacaa aactacaagc
    10141 tttatttgtg taactccagt ggtaatagtg aaaaagaaca agaatatatc cagatggtca
    10201 gccaaaataa agtagacggc atcattggaa ttacttattc agatatcgat gaatacattt
    10261 cgagtaatct gccttttgtt agtattgacc gttatttcac tgaagaggtg atttatgtga
    10321 ccgctgacaa tgcggccggt ggacgtttgg ctgcaaatga actttatcgc cgaggatgtc
    10381 agaatgtagc atatatcggt gggtaccaag aaacaccgaa tgaaacgaag agtcgtcgaa
    10441 aatattttga aatcgagtgc cgtcaactgg ggatttccta taaagcgtta gatatgttag
    10501 aacctttaga ccatctctca gaacgtgtaa caaatttttt gttagagaat cccgatatcg
    10561 atgggatttt tacgatcaac gatccaatgg cattagctgt cattgatatg cttgaaaaaa
    10621 tagggaaaaa cgtaccagag gatgtccaag ttatcggatt tgatggtcaa aagatgacag
    10681 aaggagatcc ttatctagtc tcaacgattg cccaacctac aaagcaaatg gcggaagtgg
    10741 cagtggcaaa attaattgct gtcattaatg gagagacagt agaaaaacgt accgtattgc
    10801 cgattgaatt tggcgaaggt gacacaacga aacctttagt gagaaacgat tttgcttagt
    10861 gcaaatacgt ttcttttttt gaaaatgagt gaatgaaagg tgtagataaa atgttatatg
    10921 gtggaataga agccggtgga acaaagtttg tctgtgggat tggtaatgaa caattacaag
    10981 tgatagaacg gaaaagtttt cctaccacaa caccagaaga aacgatggag caagtgtttg
    11041 atttctttga taagtacaaa ggaaatatcg catcaattgg tgtaggagca tttgggccta
    11101 ttgatattca aatcgaatca agaacttacg gatatatcac caacacaccg aagttagctt
    11161 ggcaaaatta tcattttgta ggagctttgg aaaacgcctt gtcggttccc atctattgga
    11221 ctaccgatgt aaatgctgct tgttatggag aatatatggc agggtatgga aaaggtaaaa
    11281 atagtgttct gtattacacc gttggtacag gtgttggtgg tggtgctgta gtggatggga
    11341 aaatagtcgc aggttttagt catcctgaaa tgggacatat gctcttacaa cgtcataatg
    11401 aagatcaatt tgaaggaatc tgtccttttc atcatgattg tttggaaggg ttagcatcag
    11461 gacctgcgat tgaaaaacgc tttggaagaa aagcccagac tatctcattt gatcatcctt
    11521 attggacgat tgaggcagat tatcttgctc agtgtgtgta taacacaacg ttactatatg
    11581 caccggaagt aataatttta ggcgggggtg taatgaaaca gccgcaacta ctcgagttga
    11641 ttcgccaatc gtttaaagaa aaaatgggtt cctacgtgga tacgcctact ctcgaagaat
    11701 atctactaac tccaaggctt gctgacaatg cgggcttgat tggctgccta gctttagcaa
    11761 taaagaaact caatcattag aatagtattt attagaacta ttcataacat accggaagtt
    11821 taaaagggta ttctgaaact taaatagtct caattcattc ttctttcttg agaatctgag
    11881 tagagacttt tattctgttt caaattaata taattacgaa atgatattga gtacaagaaa
    11941 gttcttttta ccagatcttt taaatcgaaa aatctgatat atatatttat agtactaaaa
    12001 atctgattag ataaaaagct aattcaatag gaaaataaag gtagtgaata aaagcaaagc
    12061 ccttgttagt agttaatcta acaagggctt tggtagggaa aatggtagga aaccatctta
    12121 ttttttacga aaatcaggaa ctttagtaat tgaataatgt tgatttatag gtattcctca
    12181 tccatattat gttggctctt tattcccact ccactgttgc aggtggtttg ctggtaatat
    12241 catacacgat acggttaacg tgtgcgactt catttacgat acggacactg attttttgca
    12301 agacttccca agggatacgg gcaaagtcag cggtcatgcc gtcaatagaa gtgacagcac
    12361 ggatacctac agtgtagtca tacgtacggc catctcccat aaccccaaca gagcgaatgc
    12421 ctggtaggac ggtgaagtat tgccagatgt cgcgatctaa accggcattg gcgatttctt
    12481 cgcgcaagat agcgtctgat tcacggacga tttctaattt ttcttctgtc aattcaccaa
    12541 ggacacgaat acctaagcct ggtccaggga atggttggcg ccaaacgatg ctgtcaggca
    12601 tgcctaattg ggtacctaat tcacgtactt cgtctttgaa caatgtgttc aatggctcga
    12661 tcagcttgaa ttgcatgtct tcaggtaatc cacctacatt gtgatgtgat ttgatggttt
    12721 gggcagtttc agttcctgat tcgatcacat cggtataaag ggttccttgt gcaaggaaag
    12781 aaacgccttc ttcaccggca agtttggttg cttcatcatc gaagacatat acaaattcat
    12841 ttccaatgat tttacgtttt tcttctggat cagacacgcc agcaagtttt gataagaaac
    12901 gttctttggc atcgactttt ataatgttca agccaaattt tccgccaaga ctttccataa
    12961 cttgttctgc ttcaccttta cgcaacaata cgtgatcaac gaagatacat gttaattgat
    13021 cgccgatcgc tttttgtaaa agaacaccaa caacagagga atctactccg cctgaaaggc
    13081 ctaataaaac ttttttgtca ccgacttgtt cacgaatttt tgcgatctcc atgtcgatga
    13141 agttatccat tgtccagtca ccagtacatt ggcagacatc tagtgtaaaa tggcgcaaca
    13201 agtcatttcc atattcggaa tggcgaactt ctgcatggaa ttggattccg tacattttgc
    13261 gttctgcatt ttggatcgat gcgatggggc aatctttgct ggtagcaacg acatcgaaat
    13321 cagtcggtgc ttgggtaact aggtcaccat gactcatcca aacggtttgt ttttcaggag
    13381 tgttttggaa aagggccgct tctgcagaag tgatttccaa ttctgctttt ccgtattcac
    13441 ggtttttggc tggtttaact tttccgccta atttataagt aatcaactgc ataccgtaac
    13501 agattcctaa aactgggatc cccaaatcaa aaatcttttc atcaatacca aaggccttct
    13561 catcataaac actgtttggt ccgccagaaa ggatgattcc tttagggttc attgccttta
    13621 cttcttctgc ggtaatgcgg tggctcatca actctgagaa aacaccgaac tcacgaatac
    13681 ggcgagtgat cagttggtta tactgactac cgtaatcaag tacgataatt tttggctctt
    13741 cgtcaaatcg tgttggtgaa gacaaatttg aataaaactt ag
//
LOCUS       DACDXE010000080        13706 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.080, whole genome shotgun sequence.
ACCESSION   DACDXE010000080 DACDXE010000000
VERSION     DACDXE010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.080"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..902)
                     /locus_tag="IWM23_002387"
     CDS             complement(<1..902)
                     /locus_tag="IWM23_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAP9884992.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="IWM23_002388"
     CDS             1185..2153
                     /locus_tag="IWM23_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9884993.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="IWM23_002389"
     CDS             2266..2544
                     /locus_tag="IWM23_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAP9884994.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="IWM23_002390"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="IWM23_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAP9884995.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3010..3756
                     /locus_tag="IWM23_002391"
     CDS             3010..3756
                     /locus_tag="IWM23_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAP9884996.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="IWM23_002392"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="IWM23_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAP9884997.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="IWM23_002393"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="IWM23_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAP9884998.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="IWM23_002394"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="IWM23_002394"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAP9884999.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="IWM23_002395"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="IWM23_002395"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAP9885000.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="IWM23_002396"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="IWM23_002396"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAP9885001.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="IWM23_002397"
     CDS             8493..9035
                     /locus_tag="IWM23_002397"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAP9885002.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="IWM23_002398"
     CDS             9058..10614
                     /locus_tag="IWM23_002398"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAP9885003.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="IWM23_002399"
     CDS             10628..11530
                     /locus_tag="IWM23_002399"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAP9885004.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="IWM23_002400"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="IWM23_002400"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAP9885005.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="IWM23_002401"
     CDS             12973..13395
                     /locus_tag="IWM23_002401"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAP9885006.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2452 c         2988 g         3736 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctgcgacaag actttt
//
LOCUS       DACDXE010000081        13429 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.081, whole genome shotgun sequence.
ACCESSION   DACDXE010000081 DACDXE010000000
VERSION     DACDXE010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.081"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..914)
                     /locus_tag="IWM23_002402"
     CDS             complement(<1..914)
                     /locus_tag="IWM23_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAP9885007.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNHTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="IWM23_002403"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="IWM23_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAP9885008.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="IWM23_002404"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="IWM23_002404"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAP9885009.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="IWM23_002405"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="IWM23_002405"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAP9885010.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="IWM23_002406"
     CDS             4440..5321
                     /locus_tag="IWM23_002406"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAP9885011.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="IWM23_002407"
     CDS             5318..6034
                     /locus_tag="IWM23_002407"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAP9885012.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="IWM23_002408"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="IWM23_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAP9885013.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="IWM23_002409"
     CDS             6751..7752
                     /locus_tag="IWM23_002409"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAP9885014.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="IWM23_002410"
     CDS             7749..9110
                     /locus_tag="IWM23_002410"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAP9885015.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="IWM23_002411"
     CDS             9070..10221
                     /locus_tag="IWM23_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAP9885016.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="IWM23_002412"
     CDS             10196..11365
                     /locus_tag="IWM23_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAP9885017.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="IWM23_002413"
     CDS             11540..12919
                     /locus_tag="IWM23_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9885018.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="IWM23_002414"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="IWM23_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4486 a         2340 c         2922 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatggttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatatgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caattcttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa accagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcaaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DACDXE010000082        13404 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.082, whole genome shotgun sequence.
ACCESSION   DACDXE010000082 DACDXE010000000
VERSION     DACDXE010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.082"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            79..1125
                     /locus_tag="IWM23_002415"
     CDS             79..1125
                     /locus_tag="IWM23_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAP9885019.1"
                     /translation="MTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPILSG
                     NEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQAPNY
                     NLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGISGN
                     KDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGPQMS
                     KTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSWGNY
                     VVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDSSLS
                     QSMLVDPKTLLFGD"
     gene            1474..2106
                     /locus_tag="IWM23_002416"
     CDS             1474..2106
                     /locus_tag="IWM23_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAP9885020.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2694..4133
                     /locus_tag="IWM23_002417"
     CDS             2694..4133
                     /locus_tag="IWM23_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAP9885021.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4428..4646
                     /locus_tag="IWM23_002418"
                     /pseudo
     CDS             4428..4646
                     /locus_tag="IWM23_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174271.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4895..5110
                     /locus_tag="IWM23_002419"
     CDS             4895..5110
                     /locus_tag="IWM23_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885022.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5327..5583)
                     /locus_tag="IWM23_002420"
                     /pseudo
     CDS             complement(5327..5583)
                     /locus_tag="IWM23_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(5770..6681)
                     /locus_tag="IWM23_002421"
     CDS             complement(5770..6681)
                     /locus_tag="IWM23_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885023.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6700..7704)
                     /locus_tag="IWM23_002422"
     CDS             complement(6700..7704)
                     /locus_tag="IWM23_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9885024.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7701..9824)
                     /locus_tag="IWM23_002423"
     CDS             complement(7701..9824)
                     /locus_tag="IWM23_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9885025.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(9829..12276)
                     /locus_tag="IWM23_002424"
     CDS             complement(9829..12276)
                     /locus_tag="IWM23_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885026.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12263..12652)
                     /locus_tag="IWM23_002425"
     CDS             complement(12263..12652)
                     /locus_tag="IWM23_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885027.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12712..13215)
                     /locus_tag="IWM23_002426"
     CDS             complement(12712..13215)
                     /locus_tag="IWM23_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9885028.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13228..>13404)
                     /locus_tag="IWM23_002427"
     CDS             complement(13228..>13404)
                     /locus_tag="IWM23_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885029.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLSLFNRIVGA"
BASE COUNT         4099 a         2642 c         2454 g         4209 t
ORIGIN      
        1 tcaagtgaaa gtagtacgat ttcaagtacc gaatcttcaa caacttcaag tagtacaagt
       61 gaaataactt cttcgaaagt gacaaaacca aaccatatag aatcttcaaa aaaggaagcg
      121 ccaaaaacgg aaggacaaac aaaaactttt tcgacaccac agcaaataga atcaacagaa
      181 tctttccaat cacaaccaat actatctggt aatgaagaaa ttcattttga caaaaatgaa
      241 tctgtagaaa attttattat gaaaattggt gagtctgcga gaaaaattgg tcagaaatat
      301 gatttatatg cttctgttat gattgcacaa gcgatcttag aatctgccag tggtcaaagc
      361 cagttagctc aggcaccgaa ttacaattta tttgggataa aaggaactta taatggaaac
      421 tttgtcatta tggtaacaaa cgaagatttg ggtaatggaa cgctttatac gacccaatca
      481 aaatttagag tgtatgaaaa ctatgaggaa agctttgaag attatgccaa attattaact
      541 aagggaatta gtggaaataa agatttttat gcaggagcat taaaagctaa tagtaaaaca
      601 tatcgagaag caactaaatt ccttacagga cggtatgcaa cagatacaca atactatttg
      661 aagctaaatg aattaattaa aacctacgat ttaaccaact atgataaaga agttaaaggt
      721 ccacaaatga gtaagactgg ataccaagtg cctattcata attattcaat aaccagtttg
      781 tttggaaatc gtggaaatga atttcaccga ggacttgata tggcagctac acagggtgag
      841 ccgattcatg cagttaaagc tggtaaggtt gttaaagcag agtttcacta ctcttgggga
      901 aattacgtgg tgatacaaca cgaagatgga tcaaccgcgt tatatgctca ccagcaacaa
      961 tacctagtaa aaactggaga taatgttttg caaggacaaa taattggcta tgtaggaagt
     1021 actgggaata gtactggccc acaccttcat ttggaaattt gcaaagatag tagtctttct
     1081 cagagcatgt tagttgatcc gaaaacctta ttattcggag actaaaaaag gtaatcaatc
     1141 tgacaaagta acaagaaaat ggttggattc ttagaccttt gatcaggata ttgagactgg
     1201 aattgatatc tcgattacga tgggtttgac aaataaaaca agtccattat cgaatatcga
     1261 gtgtattatt gccgtcttta tgctcatatt ttgagcaggt ttcactagat gaaaacaatt
     1321 tatctatctt gaggatttcg tgtacgtatc gttcagtctt gtattgtaaa ttagtcataa
     1381 aatcagacta agaaaaattg gaaatatttt tttctgattt attaaaagtt aattgaaatt
     1441 atatggtact tctttcttgg gagggtaggt gcagtgaagg aaagatatgg tcaccttact
     1501 gttgttgagg agattactca taatggatat aaaaaatatc gttgcctatg tgactgtggg
     1561 aatgaaacga ttgtatatcg atcaaactta ctatcaggta gaaccaaaag ttgtggatgt
     1621 ggaaattata aaaataggca taaatataaa aatttgacaa ataaaacttt tggtaagtta
     1681 gttgcaatca agccaactaa actgcgaagt gaaggaactg ttgtttggga atgtcaatgt
     1741 ctgtgtggga gaaaaaagtt agtatcggca aagcgacttc taaaaggtga tgtaaaaagc
     1801 tgtggatgtt tgaaatcaaa aaagcatcac attgaaaata aaaaattcgg attacttact
     1861 gcaaagtatc ctttggacaa taactgttca tggaaaacaa aatgggtttg tcattgtgag
     1921 tgtggcagta tgtgtgagat aagttatagt aacttatttt ttggacatac tcaaagttgt
     1981 ggatgtttaa aaaggcaaga aacaaggaca tgtgttgaag ggacagtaat agagtgtcta
     2041 acgtcaaaat taccgaaaaa taatacaagt ggtgttaagg gagtttatcg ttctaaagag
     2101 aaatagattg cgtatataac tttaaaaaaa aagaggtact atttaggatc gtatgattct
     2161 attaagagtg cagctcaagc tagaaaagat gctgaaaata aattgtttca acctttgatt
     2221 gacaaatatc gaggtaatca ttaattattg tggataataa ttaacagtga tatataaaca
     2281 tctataatat attattcata ctattgatat ttagtataat cctatggaat tgaatatcat
     2341 agcaatgtat ttgaaaagca aattggaaaa caaataggtg caacaccata aatatgaaaa
     2401 aattgggaaa aagtagaatg gaaatttgct tacgtctcac gagatagctc acactattaa
     2461 tctgaagaac aatgaagtcg acttttagaa attgctagaa gaaagtaaag cgaattatca
     2521 ctagttacta tagagtaagt tcaaataaag catctccaac ttacatggtt acatgaggat
     2581 atcaaatcga aattactcaa gcttcctatg gctagagtcg cgagaagttg ttgggcagta
     2641 gacataggtt atattgttct tagctacacg atcattagaa aggaaatcct attatgtata
     2701 gttatcaaga agatgcaaaa gtccaaaaaa atcgctggtg gattttgatt gctatatcta
     2761 tgtttacctt catgtccaca ttggatggca gtattgtcaa tattgcacta cctaccatct
     2821 cagatgatat gggcatacca atgaatcaag cggaatgggt agtgtccgtt tatctgatga
     2881 ttgtctgttc ttgtttgttg ctgtttggta aaattggcga tagccgtggg aaaatcaaag
     2941 tatttcggat tggtacgctg atctttgtct tgggatcgct gttgtgtggg ttcaatcatt
     3001 cgctaggtat tttattgttc gcacgggtgg tacaagccat tggtgctagt atgactatgg
     3061 ctacaaatgc cggaatcatc acggaagtct ttcccataca tgaacgtggt cgggcattgg
     3121 gagcaatagg tgcgtttgtt tcattaggct cgattgctgg accaggtctt ggcggcttga
     3181 ttttgtccca attttcttgg gcgtacattt tttggatcaa tgtaccggtg ggaatcgtaa
     3241 cgatgttgat tgctgaaaaa ttcttaccaa aagatatcac catgagcggc catacaatcg
     3301 atggaggggg ctttgctgcc attgcggcta cgattatcag cttttttgga gcaatctttt
     3361 tggggcaaga aagtggcttt ttccatccag tcccccttgt actgttcgta atttcagtga
     3421 ttgcctttgt cagcttttta aaagttgaaa agaaagcagc ctctccattg atttctttcg
     3481 gtatttttaa gaacaagatt tttaccatga gcttgattac agccttgttg attttttctt
     3541 ccaacttctt tgtcaatgta gtgattcctt tttatctgca aaatgctcgc gggttgagtc
     3601 ctagctatgc tgggctgttg atgatggtct ttcctttgat gatggtcgtc ggggcgcctt
     3661 taagcggcta cctgaccgac cgggttggcc cggagatttt ggtattagta ggcttgggct
     3721 tattgtcggt aacccaggtc atgtatatgt ttttgtcagt ggactctcct ttgtggctat
     3781 acattgtcgc tactgccatc atgggtgccg gcaatgcctt gttccagtcc cccaataata
     3841 cgatggttat gagctcggtc actatcgaaa acttgggact ggcaggctcg atgaattcct
     3901 ttgcccgaaa tttggggatg gtcagcggga tttcgctttc tacgacggtc ttatatcaag
     3961 cgatgtctgc taagatgggc cagcaagtaa ctaactatat cgcaaatcgg ccggatgtct
     4021 ttttatatgg aatgaagatt acctttttag gcagctttgt tttgtgtttt gttgcctttt
     4081 tcctgactat ctggcgtatc aaaaaacaag gaggtattca gaaaaatgtc tgaaaaaacc
     4141 actgttgaag aatttttgga ggcctgcgta tgggtgagtg ggatttccat ggatgatgga
     4201 agatgaagtg aacttctaaa agccgaaaaa ctcaagtatt atcttcaaaa tactagtgct
     4261 caatgagatg cttgatttga tcacaagaag actgcgaaag ccgctggcaa ccgcaagcta
     4321 ggtatcccac cgatggacta cgttttagag gctactggtt atgaatacgg agccaacgct
     4381 ctcatctgca taagaataca tcacccagat tatgtaaaga ttttcgatac ttctgtcaaa
     4441 aaatcattaa gatagataaa tgatttccgt ctagtcaaat tggttcagaa tgtagacaca
     4501 cagatggtaa gaagtcactc acaattcgag aatggacttg tcctatttgc cgtatacatc
     4561 ataatcgaaa cgtcaacgat agtatcaata ttttgaccaa gggctatgaa tacaagcatt
     4621 gccttagaaa acatcaagaa ccttaggaac aacggggata gcttggttaa taagagatac
     4681 cgctgtttag gcttgcaagt atactctttt atcaagaatt ccgtggcttt agtcatgaga
     4741 ggcttaatac tatgaaaatt ttctttagca tttttgattt aggttccttt tctaattata
     4801 ataagatgac cactacagta acctactgaa atatataaac agccactgta gtaccatttt
     4861 caaagtagtc tatgagagga gaatcttcat tcttatgaac gctggaattg cggatatgaa
     4921 tttaataaaa aaaaccctta atgattttac ttccaattca atatcaaaag gtactggtat
     4981 caatttaagc acaataaaaa agctgaaaag tggtgaaaga tcagtggaaa agctgaatct
     5041 tttagatgct ataaaaataa cggaatttgc tatgaaaaat ggaaaagctg agattgaaat
     5101 atggagataa ttatcgtcta gtgaattact tgccacttag ctgaatttaa cgatccttgg
     5161 tgcttgaagg gggagattct tgggaataga gcatacttgc aagcctatgt ctttactaac
     5221 aacgatatct cttattaacc aagctatccc cgtagttcta aggcttttac ttttatctaa
     5281 gcaataatcg tattcaaagg aaatttttga cccttagaaa gtctcatttt atcagacgtg
     5341 agcttaggca ttgctgaatt ttgttaattc ggttttaggt tgtgctttga gcataatatg
     5401 aaagtattct ttatcacgat ttcattcatc taaggtaatg tgatgtgatt ctgaaattct
     5461 ttcaaaggca gtttttgcat aagctgacat tacatcatca atgacatgtt tacaatgttt
     5521 cactactaaa ataagggggt aaccaagaag aaaaactgaa tgattatttg tatctaattc
     5581 catggtatat caaccatttc ctatatagac tgattatgac atggattaaa ataaaaaggc
     5641 actatgtctt aattgtcggt tttcgagtta ccgaatggca ttcatcaccc acctatagag
     5701 gatgggcgac ttctgcctga aataagttac actacttata acttctaata aaattagatt
     5761 gagtcattat tatttaacat caacgatttt ccagtttcca tttttttgta atactaattt
     5821 gtactgaaag tagctagtgg tttttgatac attatcaagg tattttatct ttgtactcac
     5881 tagaaaattt tttccgtttt gtagataaac tggattctct aactccgaaa actttaagtc
     5941 aatattgatt ggtcgcatcg caccattttc aacataatac tccaattctt tttcagaagc
     6001 tgatggataa agtttaaaaa atgtttctaa aaaatcggtg atttcttccg ttgttttggc
     6061 atcaatttct gaatcgtttt ctacttgttt tgcttcatat tttgccttgt ctggctttcc
     6121 aatcattgtt ggcaaactag ttactaaatg attcctgttt tcatcttcaa agattgtcac
     6181 tcgatatgca gaagaaattg attgcgtctt ttttccctct gtaatatgtt gctccactaa
     6241 aaatgaaacg acaaattctt ccgagtgttt ttcaacatct agaattttta cagattgaac
     6301 ttcggaactc gtgggaatat ctgctcgaac catatcttgg attaaagcga gaccgttttc
     6361 tgtaaggtac tgttcgagat tactcattct cttttcaatt gcttctttgt tatttttcca
     6421 tgaaaaatat tctttcgcaa aatcctctgt aaaactttca acgccagaag tattcactac
     6481 cttctctttt ataaccactt tttcatgagt tgtatgttga tcaatggcgg tgaaattttt
     6541 ataaacgcca aaagcaagac tacaagacaa taaaagccaa agggcaagca ccatttttcg
     6601 atgcttaccc acggatacaa ctcgttcttt tttctttttt gggctctttt ccttttgatt
     6661 ccgttcaatt tttatcttca ttctttttcc tcttttctct tatttgtatc ggccagctcc
     6721 gataagatgc tgccgccaat agttttccgt tagattcgtc caccccactg gatctcctgc
     6781 atgaaacatg cgattctccc ctgcgtaaat gcccacatgg gtcacataag taccggcatc
     6841 ataggttgaa tgaaagaaaa ctaaatcacc tggtttggca tcttttagat caatatgagt
     6901 catggcatca tattgatcct gagccacccg aggaagtttc aggtcaactt tggcataaca
     6961 ccattgggtc aagccactac aatcaaaccc cgtttctgga cttgagccac caaacacata
     7021 ggccgttcct tcatacttaa atgcctcatc aaaaatttct tggactattt tgtcatcaaa
     7081 ctgagttgta gttaggtatt gcttcactaa taaacagtaa aacatattgc cataattata
     7141 ccgccaccca ccattttcct taatcgcgat tgggttgggg taatcgactt tttgaccacc
     7201 tgatttttcc tttgaaaaag attcagctaa agcaaaacta tgcttcttcc cgtttttccc
     7261 gacatgatct agatacccgc caccatagtt ataggcctga ataatactat cttgatcaca
     7321 ccctagttct ttggaacgct taaccaaaga cgcaaaatag agacagcctt gtttgatgga
     7381 tgcctcggta cttaatgaat taggtggtaa tccggctgac tcagaacttt gcataacgtc
     7441 ttccattgtg ccaccagatt ccacttgaat aatcgccaaa agaattggaa tatactctgt
     7501 tatatccgat tctttggcat acttttccac cattgcacgg tgtttcaata cttcttctga
     7561 gacactagtt ccgccaggag aagtgccaga actaccattt ccctcactgt cttcaccaaa
     7621 caacaaaccc acacacatca aaatacccat aaaagagatg gtcagaaaag ttaatgaaat
     7681 aacacccact ttcttcaagt tcattttttc tcacctgacc ttgattttgg acgatggttc
     7741 attctttttt gtactttttg attggtctgc tttggcagag tggacctctg tttcacttgt
     7801 tggaaagaat ttttcttact tgctgattgg cttttccctt ttgaaatgat ttcttgacta
     7861 gttagttttc gtttgatttc gggattcaga tttgctctca caatccgctt tggtgcttct
     7921 tttttcagca tcatatcttt ctgaggttga gctgctttat ctattggaat attcgccctt
     7981 ctttttccct gacgatttgt tgcttcactc agtatcttgc gtttttcgtc cattttcgca
     8041 tttcttcgtt gtcgttgttt ttctcgcaaa tccactcgat ccgctttttc ttggaacagt
     8101 ccgcgcttga attcttctgg tgcttttttc gacttttcga ttcctttatg gagattgtat
     8161 ttcaaattcg ttggcgtatt ccttacctga tcctttactt gtttagtctt atctttcacc
     8221 aaagcttggg tatccaaaac tttgccgatt tttctcccag ccaaattcat tcgggattgt
     8281 ttcttattat ttgaaactgg attctcctta gttaagtcag atagtatttc gtgatcgttc
     8341 ggtaatcgtg aattctttcg tagagaagaa ccggatggct taaatttgga ttttgattgt
     8401 tcctttgcca acttatttcc gactgctgca ccaccaccaa aagccaaagc tcgtcccaca
     8461 tttcgattga gttgccgcaa ttttcgattc attaacattt gcggttttcg catgacgcgt
     8521 cttcccattg actggctatc attactctgg aggttgaaca tactcataat gtcgcccaat
     8581 ttgaaataga taccggcaaa ggtcacaatt tgcagaaaag caaccatgaa aaatggatag
     8641 gttgccgaaa tattgaaaaa catcgtggag atgctaaatg cagtcgtaat caccaaagta
     8701 acacccgctc gcatcataat cgtattgaat aaacgaataa tcgccttctt tcctaaactt
     8761 tcataagtcg gcaacatgga caataagaaa ctaattggca gaaacatcgc aaagataatg
     8821 aagagaattt gcgagaacaa cataatccct gttagaagaa aaacaaagaa ggaaatgcca
     8881 atattgaaca ataccaaaaa gaccacggtc cctaaacggt tcatggtctt ggtaatactg
     8941 agattcgcat tgtcattgtc ttcaatctct gccttgaccg cttcttcccg atctttgcct
     9001 ttgttttcat ctggactcac agatagaatc ttgttgaccc ggtcttcacc aatttcttct
     9061 atgttggaat catcaaattg cagtagtaac caaggttgtt ggacttgaat cgaaaacaaa
     9121 ctatcccgaa tcaaatccac actatctttt ccttggcttt ctgaattggg tacaacgatc
     9181 tcggttccaa gggtaagggc cgcttcacta acatctgagc taaagtcatt gatttttgtg
     9241 atataggtag gcgcataagc aatgaacgaa cccgatagta gaaaaatcat cacaaaatta
     9301 atcacggctc gaactgcttt cgtggtttct cgttttaata atccggtata cgccacatac
     9361 acccccagag ctaaaatcat caataataga aacccaaaat aaaatccgga ggtctggagt
     9421 ccattttcag taataccagc caatgtttgg atattctttc caatactttc agccgtatcc
     9481 gaaatgaaat ctaatttgta agcctcttga accacataac ccgtggcgtt cgacaaatac
     9541 aaactcaccg tccaaatgaa attagtaatg gcataaagac catacatcac gcttttccct
     9601 aagccatcac cccaattcca aggcaaccaa tcccaagagc tatccacaaa gaaatctagt
     9661 tgatagttgt tcagtgaata tttggaatat agatttcctg cttcaactgt atcatcaact
     9721 agccctgtcg cctcggctac cgtaccaact aaactcaata gaactatcag agtgagtatg
     9781 ctaataaaaa cgattcccac aatccgaaaa agtttctttt tcatcccctc actccattct
     9841 ctcttgaaca ggtggacggg tatcaaaggc atggaagagt tcttcaaaga ctggatgtac
     9901 ctgaaccacg cctacccgac catacaaatc ctgcatcaag cattggccat tttccaaatc
     9961 tcgtaggcgt ttttgattgc tttcatcttc tcgatccaag ccaaagaatt ccaaggtttt
    10021 cttgatttca tgaatatcgg tactgcgaaa cgcaaacttc aacccgatat tattcttcaa
    10081 gcgttcatcc agtaaatcat cactattttg cgtgacaaag taaaccccgg cattcatagc
    10141 ccgcccagca cgaacgagct tattcgataa tgttttccct tgcgctactt gaagaaaact
    10201 ccacgcctca tctaagtcta caattttgaa gatttctcga ttactatgga taaagtccaa
    10261 ggcaaaagtg gaaatgacga ttagcatgga aacactaagc aattccatgg tggtatattc
    10321 ttcaaaagtg gtttctgcat ctggtaaaac caaatccgct acttgaatga tatttaactg
    10381 cctatctaaa ctaatcgact gggtaatcga accatctgag aaaagcaaat gggcaaaatc
    10441 ataatcggta aaggactcga tgtgatccgc gatattttcc gcaactggtg agccttcttt
    10501 tcgaagctca ttaattactt gtaataatcc ccgttgattg ctttgggtaa ccgtccgaat
    10561 ggccttacgc aacactggaa acttctcccc atctcgacta gaaattcctg ttaaaaaggt
    10621 cagaatatca attgctagac tttcagaatc cttctttttc tccataatca cataaggatc
    10681 aagtaatcct tgattttttg gcttactagt taagttgaga atattgattt catccgcaat
    10741 ttctggcaag gtttctttcc agccaccccg ttcccctttt ggatcgacaa tcacagcttg
    10801 tccaccaaaa aggacggcgt aataaactaa gagattgtta ctaaaggact ttccgccacc
    10861 gagtgaaccg aggaatgccg ctgctagcgc attggtaact gaatccttga ccccttgtga
    10921 ggccaatgcc ggtttcaggt aaacattgcg ccccgtatct acgttataac caaagtaaat
    10981 gccttccaac tcccctaaca tctgagtggc gccaaaacca agtccggcga gaaaatcaga
    11041 cgtaacatac tggatataat cattcatgta tcgcttacta gaaggtaaga attcttcatg
    11101 tagccccatc atatcgccaa aaggccggac tagttttaca ttggtatcat cataaaaatc
    11161 tagcacttca tcacaccggc gctttagttc atctactgat tcggcactta ctcgaaccac
    11221 atagctcagt ttgtacatgg attctttgga ctgatccaag gtagtttcca attcatctac
    11281 ggaatccaat gcatctaaga cattggaatt tgtttcattg tcagattgat aggcatggtt
    11341 gtccaagtct tttaattcct ttttcttatt gcgaacggta gcaagtgcct ttttgttcgt
    11401 cacaatctcc acattcatac tagtgtcaat tgggaatgta aattgttgtt gctggtaata
    11461 gaaaagttct gaggagggaa actccatttc ccccacaatt gtattaattg ttaagtacgc
    11521 tacatatgat tcatgattct catgttccat gcataagtac cggggctttt cttcaatcaa
    11581 acaacgactg ggacgtaata agtcgtaacg tttcaccaaa gtttccgact tcagcttttt
    11641 ctttggtagc tgaaattcat attcttcaaa tggtgtaccc ttttcgccat agatatgttc
    11701 aatcaaatac atcagatcac tcggtgtgac tcgtctgact ttaaatcttc gagccaattt
    11761 actttccatt agtttttcaa gcttggtata tcggcgaatt tcttcattgg ataaggaaac
    11821 aaaatcgccc atcaaatgat gattcactcc atagacaaat tcttgaaagc cggagaagaa
    11881 agatttcttg agattcttta gattgacctc ttcatctgtc gcaatcagtt tgaagccaat
    11941 aaaaaagcga taatcaatct gactatcgcc aatcatggat actaatgcct ctgtctgtaa
    12001 atcaattcgt tgtttcgcta cttctgttaa tcgcccggta atttcctttt ttgatcgttc
    12061 ttgcgtggca cgaatactac tttctgtggc aatttgtaag gcatgaatct tgccttcacg
    12121 attttgcgca atcaattgac gaaagttatc gtggacttga tatttctgtt caggtgacaa
    12181 aaaggagtaa ttgtaaggta ttaactcata gtaggcaaaa cattctccct cttgattaaa
    12241 gacaagatta ttttcaatat acttaatcgg gtacatagtt cacactcctt accagagtaa
    12301 tgggttcgtc ccatcgtttc ttctgttctt tgactttctt tcctgcgaaa gtgaccttaa
    12361 atcgtaagtg atagctaata aaagatcgta aaaatcccaa tggtttctta ccgtcaaacg
    12421 ttttttgcga catgaaccaa gtaactgcca ccggtatacc taagtatttt aaaaaggccc
    12481 cctctatcat agatagtgga ggcacatggg caaatacgat gacaaacagc aaggataaaa
    12541 caaaccaagc catctggtta aaagtcacag gaaaaggcag ttgaaagtca ttaattgcat
    12601 aaatgacctt ttcaactgcc caaatactgg tgtagctttt tattttcttc atcaaatcat
    12661 tcctttaaaa agaaggatag tttagaaatc aactaccctt caataatttt gttaatatgg
    12721 atactcaaaa acacctcgac tcgttaccaa ataattgcct ccaatctcca agtctctacc
    12781 acacgcatca aaatcaatat acgtcttcaa gtgttctggt acttctccaa aatcaccagc
    12841 ttctacaatc aagaaatatg ctacatcact catatcatcg caatctggat aatagataat
    12901 atcatccaca tgctctacca tctcctcaaa agaattgaag aaagcatgtt gaatctcaga
    12961 agcaacttca ccaactggcg ttccttcaag ttcctccgct aagccacaca accgattaat
    13021 ttcctcaatt ggcgtatact catcaatctc aaagggaagt tcataatcat gaattgcata
    13081 ttcctcgtat tcgtcattta acccaattcg ttctttcact tcatcaaaat cgacaggtgg
    13141 ggtaaaccac gcccccacca attcaccttc gttatatttc ccgaggttcg caatataaac
    13201 acgcatctgt tccatcgtgc ttccccctta cgcaccgaca attcggttaa acaaactgag
    13261 taagacatcc ttcaccccag aagcattaaa gaccaatccg accgcaatca aagcaatcac
    13321 caaaaagcca atcagtttgg aaaactcccg cttaaatcct aaaaacaaac caatgaccac
    13381 aattgccagt aagactaagg attg
//
LOCUS       DACDXE010000083        13400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.083, whole genome shotgun sequence.
ACCESSION   DACDXE010000083 DACDXE010000000
VERSION     DACDXE010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.083"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(16..708)
                     /locus_tag="IWM23_002428"
     CDS             complement(16..708)
                     /locus_tag="IWM23_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9885030.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            793..969
                     /locus_tag="IWM23_002429"
                     /pseudo
     CDS             793..969
                     /locus_tag="IWM23_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348541.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
     gene            complement(1145..1714)
                     /locus_tag="IWM23_002430"
     CDS             complement(1145..1714)
                     /locus_tag="IWM23_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9885031.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1788..2111)
                     /locus_tag="IWM23_002431"
     CDS             complement(1788..2111)
                     /locus_tag="IWM23_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885032.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2265..3077)
                     /locus_tag="IWM23_002432"
     CDS             complement(2265..3077)
                     /locus_tag="IWM23_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885033.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3431..4279
                     /locus_tag="IWM23_002433"
     CDS             3431..4279
                     /locus_tag="IWM23_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAP9885034.1"
                     /translation="MIVALLAGGHVLFEDIPGVCKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4424..5365
                     /locus_tag="IWM23_002434"
     CDS             4424..5365
                     /locus_tag="IWM23_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAP9885035.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5355..7526
                     /locus_tag="IWM23_002435"
                     /pseudo
     CDS             5355..7526
                     /locus_tag="IWM23_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IWM23_002436"
     CDS             complement(7638..9809)
                     /gene="copB"
                     /locus_tag="IWM23_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAP9885036.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9829..12015)
                     /locus_tag="IWM23_002437"
     CDS             complement(9829..12015)
                     /locus_tag="IWM23_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAP9885037.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IWM23_002438"
     CDS             complement(12015..12224)
                     /gene="copZ"
                     /locus_tag="IWM23_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAP9885038.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12237..12677)
                     /locus_tag="IWM23_002439"
     CDS             complement(12237..12677)
                     /locus_tag="IWM23_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAP9885039.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12751..13290)
                     /locus_tag="IWM23_002440"
     CDS             complement(12751..13290)
                     /locus_tag="IWM23_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885040.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3766 a         2807 c         2380 g         4447 t
ORIGIN      
        1 agaggggggg attttttatc gttgtggaat atttaaatac caacgcttgt catgaaaatc
       61 ttgtgctcca cccttagtat ttccttctgg atcgtttgtc gcacgcatca tgacgtatac
      121 tttcttatta gggaaatcac gcatattgaa agatacatga tagccaacat ttcctaaagt
      181 attataagct tgatttacgt ctggtctata aatcccatca gctcttactc tagctagttc
      241 ttttccagta ttgtagtcca taatgaaaat atactcgtat ttataattag caatgtgcca
      301 tccagctaca tgcaagtttg cgttttcgat ttctccgaac tgatcaatgt gggcgtaatt
      361 tgttccatct gtcagcgtag aatttgcagc acctgctcga gttggatcaa ttactggttt
      421 atcctccgaa gtagttggat tatcatccgt aaaaccatga gccaaatcat aggctagttt
      481 ttctttactt acacccattt cagaaagata accgtaagga tctgtatgat cgccccaaat
      541 attttgtgtt acccataaat gcgatttgat tcctggttgg ttataaggag tgtctaatgt
      601 taatggaata ccatatttca ttgctgaatc tcttgccaat tcaaagtata ctttatagtt
      661 cttttcaaaa gtttctttat catgtgtatg ttgtaactcg atttgcacag gacttttggc
      721 tcacgaggtt gtcctactct acatcttgct gatagatatg ctgaaaggaa aacaccaaca
      781 ttatgttttg ctttttcagc tagaacgaga caagcaggaa tacctttaga agatatcaaa
      841 ggttttttag gtcataaaga tgtttccact acacaagtct atgcccatat ttctcccgaa
      901 gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag aagagcaaat aaaaaagcac
      961 tcaaattgag tgctttttta agctcactat cactaaaact atcactaatt ttatttataa
     1021 gcgaacatgt ttctatcact gattttttaa atctagcgaa aaacccttat cttaaaggcg
     1081 ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg cctagaagtg cgaacatggt
     1141 tttcttatag ctcatgtaga aaaattttct tgatgatgat tttatccatc aattgagatt
     1201 gactgttttg ctctcctatg atttcagcta tgattgtccg attttgcggt tttcttctta
     1261 actggtagtt gattttccca gtttccatca actcgctaag catatccacg actccgccaa
     1321 atccggtctt tttcgtaata aaacctagtg ctaccattcg ttcatctaga ttttttgctg
     1381 taatcgtcac accagtaatg agcgagtgct gtaatgtttg ggccagctct tccatccctt
     1441 cagcattgga tacgacggaa gctgttttgt taataggttc ttcgtttatt tcttttggtt
     1501 ctttcctctc cactttcact gtttgacgaa ggacatctag ttcttgcaag agcgtttcgt
     1561 attgttcttg ggtcagcatc acgccagcta ctttttcacg attaaatata tatacgccag
     1621 ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga tcgtttcact tctgtgatcg
     1681 aagatgtggg aacttctaat ttctttaatt ccaatggatt tcctccttta ctaatcgctt
     1741 tttattatag cacagactga cttgttttga tcttttttcc aaaggtttta tcggtttttg
     1801 cctaaccagt aattgatatc ggtaagaagt ctttcgatac caaatgaagg atggtacaga
     1861 tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat gatcgatgat cactagatct
     1921 gcataccgag gcggcttatt tcgagtacat acatgtatag agatccgttt tcgatgtctt
     1981 agcgttttgt tcagtacccc agcaagtctt cttgcttctt ggtgttcata gcgaaaaaaa
     2041 aagacgattt tcacttcagg agggtggaca tctaaatacc ctcgttcctg gagtgttttc
     2101 agtaaaagca tcccccactc tactgcgggg tgtgcaagtg cttgttggat cgctagccga
     2161 aattgatata gtttatcttt ggaattggga aatttttcta atgtttcctg ataataacga
     2221 acttcttcta tcagccggtc atggatacca attacgtaac gaagtcattc ttggtaaagt
     2281 acttttgtca gctttgattt gctttcgctc tcaaattcgc tattcagcca aaacagcgaa
     2341 tcaagctttt cagtcacaat cgtcaatttc cgatagtctt gtgtctcttc tatccgtttc
     2401 caccgctttt cagtaatctg gttacagctc aagcgcaaaa ttgctagata atttttttct
     2461 tttattggca aaatctgctc ctctctaaat agcaattcag gaaacaattc aaagaatagt
     2521 gttcgactgt ctcctaaaat ctcgatttct gccggatcct ttctttgctt caataattgg
     2581 atgaacatct cagaaaagga ctgctctcca tcatctgcaa aaaaatgcgg aaaatctttc
     2641 aaaaagcaaa acagcagata tcttactttt ctttctgaac caaagatctg tgctttttta
     2701 taagaaaaag aaaggtggta ctcgccaaac cactttctca atcgctcttg cgtagaacga
     2761 tcgtacttct gctcgtaagg ttcattcaat agcagatttt tcagtaaaca aaacgggggt
     2821 gattggatca gaaaaacaaa caagagataa ggttcgctat atccttgatc ataaactaac
     2881 tgaacggttt tcttatgaag aatcagctga atgggacagt cgctttccgc caaacgtttc
     2941 agatgccgat ccactgtttt aacatcgatt ttcagttctt cagctaaatc agcttttcgc
     3001 cagatcaaat ctttcattag attgattagt gaaaataaat tctgtgcatt ttttgataat
     3061 agtttttgcc atctcatcga tcatttctca cctgatttct aaacatattg tttgatcaaa
     3121 atgggttcac tcctaaaaag ataaaaaact ccctttctat ttatttaaat acgcaaatct
     3181 tcctcattat tttttatttt actcaattta atcgtattct catttcaaaa cgaatgtact
     3241 ttaaaaaaac ataataagat cactttgctt gtatgatgta ttcccttcca ttacaatgaa
     3301 ataaatagcg attttatgag aaggtgatgt gaaaatggaa gaaaaaatag aagaatccaa
     3361 acaaaaaatc gatcagttga tataagaatt agaaaaagcg gtcatcagca aacgttcaac
     3421 tatccaattg atgatcgtgg ccctattagc aggtggtcat gtcttattcg aagatattcc
     3481 cggtgtatgt aaaacgttac tggtcaaagc actagcaaaa gctgtacagg gaaccttcag
     3541 cagagtccaa tgtactcctg atctgctgcc tagtgatatt cttggttttt ccgtatacaa
     3601 ttcacaatca aaagagtttg aatttcgtcc gggaccgatc tttaccacga tcttgttagc
     3661 agatgagatc aatcgtacga ctcctcgtac tcagtcagca cttttagaag caatggccga
     3721 gaatcatgca acgatcgaca acaatacata ccctctagac aatcattttt ttgtcttagc
     3781 tacgcaaaat ccaatcgagt acgaaggaac atatcctttg ccggaagcac agctcgatcg
     3841 ttttttattc cgactacaaa tcggttatcc ttcttttgat gacgaacttt tgctactttt
     3901 ggataaattc gaaaaagtgt tggataactt aaatgaagta ttgaacagtt atgaaataga
     3961 agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc gaagtggcat cttatgcctt
     4021 gcagttagtg tcagcaacaa gaagtcatgc tgccatccag cttggcatca gtccacgagg
     4081 aagcctttcc tttattcagg cagcaaaagc ttatgcttta atccatggga gaaattacgt
     4141 cacgcccaaa gatttgcagg atttagttcc ttacgtgttt tcccatcgcc tgatttttta
     4201 cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt catactcttg ttgcacaaat
     4261 tcctattcct gtgaggtaga ttgaggtaga caatggaaaa aatcaaagat tatttcatta
     4321 attttgtgtt atttctggta tatttcatca ttgtgctttt tgctcttgta ttcaacaatt
     4381 ctttaggctg gttcttgttg gcctttttta ctctttatgt atcatggagc ttgatttcat
     4441 taagcagctt tctaaagaaa ctttcggtta cttgtacatt gcagccactc gtttatcgca
     4501 aaaaaagtat gcaggttatt ttttccatag caaaaaagag aactttttct ttacctctcc
     4561 ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa aaacaagaga ttttgattct
     4621 tacaaataaa ccgcaagaag tccaattgct ttggcagcca aaagaaagag gatgccatga
     4681 aacattaact gtcattttta cagcctatga tagtctagga cttttcagaa aaagtgcaaa
     4741 aagagagatc tcttttcctg tgactgttct gcctgctttt tgtaagattc aagcagaaaa
     4801 attacagaga gttctggaaa aaaaacagca gctgaacgcg taccaaagag gtcttgattt
     4861 tcgggaacat cgcgcttatc gtccaggtga ttctttcaac cgaatcgatt ggaaattgtc
     4921 tgctcatact caagagtggt tataccgtga atacgattat caagaagaag aatgttctcc
     4981 tcttttttgc ttttggggtt tgcccagtcc aaaatttgaa cctctgctag acttgtattt
     5041 ttctttatgg catttgagaa aagaaaagca gccggaactt ctgatattag gcaaccgcgc
     5101 ctttcatggc aaagaccccg gtcataccta ttttgcttct ttggaagctg gcgatgagaa
     5161 agcttttttc gcacacttga aaaaatatgc aaaaaaacaa atcgtcctct ttgttcctga
     5221 gaattctcct gaagtctcag aagctattga aacattgtca aaagatcgaa cggtttatct
     5281 tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa gacaagatat gttcattgcc
     5341 aggaggtgaa tggattgacg attaaacggg aaaaatggct gcgtggcgtt tactcctttg
     5401 tgatactaag tgtaggtgct ccgatatttg tagtagctaa tcaattgcaa aacagctacc
     5461 ttcttatcct aactattggt gtcctatgcc ttattgtctg ctgtattcct aaaaaatggg
     5521 tacgtttgct gctttctttg ccagtaatga taggttgttt gtttttatat tttccttcga
     5581 agacattttc catattatgg atcttacagt ttttttcgtc agtcacagag gaattcttcc
     5641 atctatccca aggtaaactt tattatttgc cagaaaagac agcttttttt ctcataatgt
     5701 tgctgatcta tttatttatt acattgaccg tggattacga ctattggtac gcaccctttc
     5761 tttctttgat ggcttacttc atgatgctta ccgtttttcg gaaaaaagat ttactgtacg
     5821 aaatgattgc ggtagtaact gttttgttcg tgtatctggc agcaaggcaa tttttctcca
     5881 tccggttgtt gtcgggaaac actcgattcc agtcattgac tacagttctg cttatttgtt
     5941 tcttaacttt tagcgctatc ttgcctttat atcttccgaa agtacaccaa tctctcgagg
     6001 aaacgagtga accgatccga gaatatctta atgatgaagg gctttatgac acactccatg
     6061 agtatcaatt aaccggaagt gcaagaacag gcttcagtga gaatgatgaa cagcttggtg
     6121 gacctttata tgataatggc gaagttgttt ttacagcgaa ccaaaaagga aatggctatt
     6181 ggaagatcga aaataaaaat cattacaccg gttctggatg gatagaagat caggacgcac
     6241 ccatccaacc aatccaagaa ttccctgttg acatcacgag ctaccaaacc gttatagaag
     6301 acaaagaaaa cattacattg accctaaaaa aacaagtttt cttaccttat ccttatggaa
     6361 atactacctt gactgatttt tcattagcta gtggacaaac gctttactat ttgcctgaaa
     6421 gccagcgatt cattcctgtc ccgaatgaat cgacacgttc tgcaacttta caaacagaaa
     6481 ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt aaaagctgat gatttagaaa
     6541 atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa acgtattgag gacttggcgg
     6601 tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt cgaagctgtc taacagtatt
     6661 taaaaacaag cggaaatttc cgctattcca aaacggatac cgggcatact ccaaaagaac
     6721 acgattatgt ggattatttc ctctttgatt caaaagtcgg ctattgtgac aacttttcaa
     6781 cttctatggt cgtccttctt cgaacacttg gtatcccagc tcgctggaca aaaggtttta
     6841 caaatgggct cagaacaggc acagataatc aagggaatga ggaatacagc attctcaata
     6901 gtcatgctca ttcttggcca gaggtatact ttcctggatt tggctggctt ccatttgaac
     6961 caaccccttc atttgccaat ccaatgggtt cacaaccagt tgctgaaaca accgaaaccg
     7021 tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat ggcagaaact atttccagtg
     7081 agagatctga ggtcattcca tcagaagaaa caacactcac accagcttat aaaatcgtct
     7141 tattcagcct tggagtgtgt ttatccgtct tgttgttctt tctattgtta ttcagaaaat
     7201 ctctgcgctg gacaagttat cttatctatc ttcatttttt tgcttctact aatacgattg
     7261 catatcaaaa acttcttaac cctttggaaa cattggtacc gcgcaaaccc gcagaatctt
     7321 tgcaggaata tagcctgcga atagaacaag aattcaagct agatctgcgt ttcattcgat
     7381 taactactca atatgaagca gaaatatatg gacgacaagc agatccggta aaattcgata
     7441 aaaaaaccta tttagaaata acgcatataa taagaaaaat ggtatacaaa aaaattctga
     7501 caaaagggag ccgcttcaaa aaataagata aaatttctgg agaatctact tctggaatgc
     7561 cgagaaccag ctgattgagt atcgattact cgaaaaaaag gttgtgacac gttttttgtc
     7621 acaacctttt ttcattatta tctgattttc aatgtcaaag catttagagc tacaactacc
     7681 gtacttaacg acatcaagac agcaccaact gctggactta atatgatacc aattggcgca
     7741 agaattccgg ctgctagcgg aattgcaaga atattgtaac ctgcccccca ccagaggttc
     7801 tggatcattt tctttcgcgt ttgcttggct agatcaagaa aatgcaaaat atcttttgga
     7861 tcgctgtttg tcaatacgac atccgcagaa tcgattgcga tatctgtccc tgcaccgatt
     7921 gccatcccga ttgaagcacg ggctaaactt ggcgcatcat tgatcccatc acccaccatg
     7981 attacttttt tcccttggtc aaggtatttc tggacaacag cttctttatc atctggtaat
     8041 aaaccgccat aatattcttc gatccctaaa taatctgcta ctgcttgggc agcgttttta
     8101 ttgtcaccag taagcatgac aggagtgatt tttctttctt tcaatgttcg gataaattct
     8161 tttgcttctg tttttactac gtcacctaaa gcaacgattg cagctagatg attttctaaa
     8221 atcaggaagc tgatggtatt tccttgttct tggtaaggtt tcaataatgc ttgttctacg
     8281 tttaatccga gacgctcaac ttctttttca ttaactattt tcacttgttg ttttttgact
     8341 gttgcctcta gcccgactcc tgaaagattt tttagatttt cagcttgata aggcttgatt
     8401 tcatgttctt tcaaataatt cataatgcca acagccaatg gatgattggc attcgcttcc
     8461 aaggctccta gatattgaag gatttcattt ttattgaacg catcatcaag tacttcgaca
     8521 cccgttacag taaattttcc ttctgttagt gttcctgttt tgtccagcat gatcacatct
     8581 agatcattgg cttgctccag tgcattgcgg tttttcaaaa gtaatccgtt tttagctgct
     8641 attgatgtgg aacgagcaac aactaatggg atagctaaac ctagagcatg aggacaggcg
     8701 atgataaaga ctgttaccat acgttctaat gcattaggaa gatctgtcaa gaacaaccag
     8761 gcgatgaatg ccaggatacc cacaattaat gcgacataaa acagccattt tgccactttg
     8821 tcagataatg actctagctt ggatttttct ccttgtgctt ttcgcaccat ttccataacc
     8881 ttcgctagat aaccattttc accagttcca gttacttcga tttcgatcgt tccatcccca
     8941 ttgatagaac cgccaatcac tttgtcattg gcttgttttt tgattcctct tgattcgcca
     9001 gtcacagctg attcgtcaac aattgtttca cctttcaaga ttacaccatc tgttggcatt
     9061 ttatcaccag accggacgat caaacgatca ccttcgttta cttctttcaa tgaaactgtc
     9121 tcttcttttc catctttcgt caagcgtttc actgattctg gcagtaattc tgctagtttt
     9181 tgtaaagcat tgctggcatt cgacacagca ttcatttcaa tccagtgacc tagtagcata
     9241 ataacgatca aagtagccaa ttcccagaag aaatccatga cgtgtgtatg tggattgagc
     9301 agatttgcga taaacgaata gacactataa atatatgcaa ctgtaatccc catcgcgatc
     9361 agtgtcatca tagcaggact tttttgtttc aattccatct tagctccact taaaaatggc
     9421 tgaccgccat agataaatag tatagttgct aagatcaata cgagccaatc agaaccagga
     9481 aacgttactt gaaatggaaa ttccattccc atcattggtg aaaaaagaat gattgggata
     9541 gctaggataa gcgatagcca aaatttttgt ttgaaattcc ccatgtgcat ggaatgatcc
     9601 atgccgccgt gttcgtgatc catgtgcatc gaatggtcca tttgtttgct gtttgctata
     9661 tctccatggt tatgtcccat gtgcatggaa tgatccatct gactatggtc atgtttcatc
     9721 tccatctgtt tttcatgaga ttctgtactt tctttgttta ctacttcttt tttatgctca
     9781 tgttttttct ccatatcgtt cccattcatt tttactccat cccttctctt actttttcat
     9841 gtgtcgattt aagcacaggg agttcaacaa tacactgacg gaactaaacg ccatcgctcc
     9901 tcctgcaatg attggattca aaaagcctag tgcggcaaaa ggaataccaa tcgtattata
     9961 gacaaaagcc cagaaaagat tttgtttgat ttttctcaat gtcaacctag acaactcgat
    10021 tgtttgtgca atggatgcca aactgctatt catcagcgtc acatcggctg tttccatcgc
    10081 aatgtccgtt ccacttccca ttgccatacc gacatcagct aatgctaagg ccggtgcatc
    10141 attgattcca tcgcctgcca taccgacttt tttgccatct ttctgaagtt tttctacgta
    10201 ggctgcttta tcctctggaa gtacttcagc aaagatatgc tttggatcaa taccaacttg
    10261 tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc agcataaaaa catctaatcc
    10321 tttattctgg agctgcttga ttgcttgttt gacttctagt ttgatttgat cagccacggc
    10381 aatcaacccg atcacttgct tttcatcagc taaaaacatg actgtttttc cctgctgttc
    10441 taattctaac gcgtgttctt ggtattcatc aaatgatagg ttcaattcaa ttaatcgttt
    10501 acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg ctgattccag cacctgggtg
    10561 ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc agttttccgt attccacgat
    10621 tgctttacct agtggatgtt cagaagcttg ttctaaagta taaaaaatag ataggacttc
    10681 tttagaaccg actagatctg taactttggg ctttccttct gtaatcgttc cagttttatc
    10741 caaaacaatg ctgtctaagt gagcagctgc ttctaatgct tctccgcctt taattaaaat
    10801 tccatttcgt gcaccaacac ccgttcctac cataatagct gttggagtcg ctaaacctaa
    10861 tgcacatgga cacgcaatga ctaatacaga gacactatgg atcaatgcga gttgccaatc
    10921 accagtgatc aaacctgtag ctatgagcgt gataaacgcg atgactaata cgatcggaac
    10981 aaaaatccct gagatccgat ctgcgatttt ttggattggg gctttgctcc cttgagcatc
    11041 ttccaccatt tgaatgattt gcgctaatac tgtctgcttg ccgacttgtg taacttccat
    11101 gtgaagtaga ccattcgtat tgattgtccc gccaaagagc gtgtcatccg gattcttttc
    11161 tacaggaaga ttttcaccag taagcatact ttcatctatc gcactgcttc ctgaaataat
    11221 tcgaccatct gctggaatct gctcaccagg acggatgact agttgatcac caacgactac
    11281 ttcttcaatc gctaacgttt cttctttccc gtttcttatc acttgggcgg tttttgtttg
    11341 aagagacatc aattgtttga ttgcattacc tgttttcgtt tttgctgtat gttccaaata
    11401 tttgccgagc aaaatcagcg tgatgatcat actgctgctt tcaaaataaa gatcttgggg
    11461 gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag gcagccgatg ttccaattgc
    11521 taccaataca tccatatttg gtgctttcgt cttgattgca tggtacgcac ctttataaaa
    11581 acgagcaccg acataaaatt ggacaggagc agacagcact aactggacga ttggaaaatg
    11641 aaaaaagtga acaatcgccg catggcttcc taacatcata gcaatcattg acaacatcaa
    11701 cggcaaagtc aaaaccgtac tcaatataag atcgaatagc atttttttca aatacgcttg
    11761 cttttcttct gcaatttttt gtttgtgggc ttcatcgtac aaaattgcac catagccgat
    11821 gttttctacg ctttggatta atttttcagt ggtcgtccct tcatatttga cacttgcttt
    11881 ttcagtggcc aagttgactg tggcattgac cacaccaggt tgtccgttca gctctttttc
    11941 gatcctagca gaacaatttg cacaagtcat acctgtaatg acgaacgtat ccattttggt
    12001 ttctgctgtt gccatcaaat cacctctgct ttataaccca atccattgat tgcttgacaa
    12061 atcttttcgg attgaacttt tgtttcatca aacttgaccg taccgtttgc ttttttcaaa
    12121 tttactttca ctttttgcac gccgtcaagt gcgcttactg tttcttctac tcttgctgca
    12181 caatgattgc agctcatacc ttcgattgaa aattgttgtt tcattttttc ttcctcctat
    12241 tgatgttgtt tacattcaca ttgacctggg atacagttac attcgatcgt atctactggt
    12301 tgcttttgtt ccagttgttt caaaatctca ttgatatctt cttgagtcaa agtggcttct
    12361 tctactagat ccgcaatcgt ctctccaatc ctttttgcac agatatggga aaaaagattt
    12421 tccgtcgcac tgcgaactgt ttccatctcc gagacagctg gataataaat gaattttttt
    12481 ccttcttgtt ctgtccagat cacttctttt tttaccaaac gacccagcaa agttttgatc
    12541 gttgctggtt tccaatccat cgaatcagct aagatctgcg tgatttcttg ggctgtcgtc
    12601 tgtccaagtg tccaaataac acgcatgatc tcccactctg aatcactgat ctttactggc
    12661 gttttgactt tttccattta tttcacacct ttcgtttaca aatgtaatca ttatcttatg
    12721 aatacagatt acacttgtaa acaaaaactg tcaattattt ttgctctcta ttttcaattt
    12781 agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg atcagtttat ttcttgcatt
    12841 agctacttgg atcagctgct gtattttcgg ttcattgttt tcttcaaaat gccagataaa
    12901 agataaaaat tctaagtatt ccctgtttag tttctccgaa aatgattcag gcatatctgg
    12961 ccgagatttt ttgtttatct ttctttttag agaacgccaa actactcggt agatggcgag
    13021 ataacgcggc actttaagcc aaatgatctt atctgcttct ttaatccttt cctctaatgt
    13081 agaagtataa tttccttcaa cgatccatga cggattagat accataaaca attgctgttc
    13141 ttgcaaaaac cacttttttg cctgaatact gtagtctgtt gtatgccata aaaggtctag
    13201 cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt gcgagggtcg attttcccga
    13261 cccactttgt ccaatcacga tgatcttcat tcttctccct cacttctctt tggaatttgg
    13321 ttcgatcata ccacttataa cgccgcaaaa aaacaaggga ctgtgacaaa agtcgtgtca
    13381 aagtcccttg tttcgaataa
//
LOCUS       DACDXE010000084        13232 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.084, whole genome shotgun sequence.
ACCESSION   DACDXE010000084 DACDXE010000000
VERSION     DACDXE010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.084"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            168..1037
                     /locus_tag="IWM23_002441"
     CDS             168..1037
                     /locus_tag="IWM23_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAP9885041.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1246..2271
                     /locus_tag="IWM23_002442"
     CDS             1246..2271
                     /locus_tag="IWM23_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAP9885042.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2310..3260
                     /gene="manA"
                     /locus_tag="IWM23_002443"
     CDS             2310..3260
                     /gene="manA"
                     /locus_tag="IWM23_002443"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAP9885043.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            3354..5057
                     /locus_tag="IWM23_002444"
     CDS             3354..5057
                     /locus_tag="IWM23_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9885044.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5059..7092
                     /locus_tag="IWM23_002445"
     CDS             5059..7092
                     /locus_tag="IWM23_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAP9885045.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7103..7543
                     /locus_tag="IWM23_002446"
     CDS             7103..7543
                     /locus_tag="IWM23_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9885046.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(7743..8870)
                     /locus_tag="IWM23_002447"
     CDS             complement(7743..8870)
                     /locus_tag="IWM23_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9885047.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8873..9550)
                     /locus_tag="IWM23_002448"
     CDS             complement(8873..9550)
                     /locus_tag="IWM23_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9885048.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9814..11580
                     /locus_tag="IWM23_002449"
     CDS             9814..11580
                     /locus_tag="IWM23_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAP9885049.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11595..12032
                     /locus_tag="IWM23_002450"
     CDS             11595..12032
                     /locus_tag="IWM23_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9885050.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12045..13196
                     /locus_tag="IWM23_002451"
     CDS             12045..13196
                     /locus_tag="IWM23_002451"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAP9885051.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
BASE COUNT         4493 a         2300 c         2732 g         3707 t
ORIGIN      
        1 acagcttttt cacaacctct ttttataaga gtttttactc attaatttac aaaaaacaag
       61 caaaatgcga attttctcac atttttttta acaaaatcaa agttattgct tgaaagaaga
      121 agaatccatc gttaaaataa agcttgacgt agaggggagc aagaaatatg aaagattttc
      181 aattgggggc tgccacctat ttaaaaagtg tagccattcg ggtgaaagac cgagataaaa
      241 tgattgattt ttacaagaac gttgttggct ttgatttgaa aagagaagaa aacgaattag
      301 ccattttagg aacagaagaa gctggaagtg aaatgttgct gctggaagaa agtcctagag
      361 cgaatgatca catgggggtg atcaaaaagc tgaaccgttt ttcattgatt atccctagca
      421 cagaagaaat ggcagatatc ctttgtcgta tccgtaaaat gaattatcca atcaaagaag
      481 ctttagaaga tcatggacgt atggggattt tgctcgtaga cccagaagag aatgaattgg
      541 aaatttacca tgaagagaat aagaacgacc aaagagaaat tccagaaaca atggatcaag
      601 aagcattatt aagaaaatct gaagaaaaat tcagtaaact atcaacaggt tcttattttg
      661 aaaaaatcca tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac
      721 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc
      781 atgttggttt gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg
      841 gacttgattt tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact
      901 tgcagcaact atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg
      961 atgctatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
     1021 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
     1081 aacggtcata ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac
     1141 tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct
     1201 ttaagcttgg actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg
     1261 aaacagctta ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc
     1321 ataaaagaaa atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag
     1381 cagctgatga cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc
     1441 acttcattag ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat
     1501 aaatatcttc ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact
     1561 tctgatatca aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc
     1621 gcttatttga agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc
     1681 ggtacgattc ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc
     1741 tttcaagaac aagtattgga accattagga atgatgaaca gcttatttct tccttttcct
     1801 tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact
     1861 catgatccga aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat
     1921 ttgaaagaca gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt
     1981 cgttttttag aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat
     2041 ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt
     2101 tttcatactg gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg
     2161 atatttttgt caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga
     2221 gatgaaattg ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat
     2281 agctatgaaa aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt
     2341 ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta
     2401 acgataaaat cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag
     2461 aaaatggcga attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt
     2521 ttggccatcc ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg
     2581 atctatctgt acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag
     2641 gaaaaactga atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc
     2701 atcatgcaaa gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc
     2761 ttttgaagaa agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc
     2821 atgctattgg taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc
     2881 gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac
     2941 aatcgatcga tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc
     3001 gaaaagggaa ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt
     3061 gggatattaa aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa
     3121 tcgaaggagc aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa
     3181 gctgtattat tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg
     3241 cgactgaacc aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc
     3301 tgctttttct acataaaatc cgtccatcaa taaatagaag tatggtgaaa aagatggaat
     3361 acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac
     3421 atcacttaaa tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt
     3481 cattagcaac aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt
     3541 ggatgatcca gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg
     3601 gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg
     3661 ctccacaaat cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat
     3721 ttgtttctag attccacgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac
     3781 tagtaggact gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt
     3841 taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt
     3901 taccacttgt cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca
     3961 atcatggtgt attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct
     4021 tatttttgat ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt
     4081 ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg
     4141 ggattcacga aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa
     4201 tggctggtgg ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac
     4261 ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg
     4321 gggtaagtat agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat
     4381 tgaaattacc aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg
     4441 aaccatctat cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta
     4501 tgggagccgc cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat
     4561 acagctctat ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac
     4621 tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt
     4681 tggaaacaga tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag
     4741 aaaaaaaaga gatagcagat atcacagaac aaaaaaagtc gcctgctaat attgtatttt
     4801 tgtataaaaa caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag
     4861 caaaaaaagc gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc
     4921 aagaagaaac gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa
     4981 agatccctcg gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg
     5041 tgaagggaga aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt
     5101 ctcagtagcc cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa
     5161 cggactattt accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct
     5221 ttgaaaaaag cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa
     5281 ttaaaagaaa atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg
     5341 attttagccg agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca
     5401 tatcgcatca gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc
     5461 caactgcctt tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac
     5521 cgccggcttc tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat
     5581 agtatttctt tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta
     5641 tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat
     5701 agaaagcttg aacatttggt tttgatgctg acattgacga tggagcgtgt gactaaaaat
     5761 catttactga caaatgagac atataatagt ctggcaaaca aagaattact acagacagct
     5821 aaacgattgt tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc
     5881 gtcttttttg caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa
     5941 gagacgttcg atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa
     6001 gagacgaatg tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta
     6061 tctggtgtgt tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa
     6121 aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt
     6181 tttcaagaaa aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat
     6241 tcattggaac gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga
     6301 ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa
     6361 attcatttct atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt
     6421 gttatttcta catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta
     6481 ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa
     6541 aggcaaaaaa ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa
     6601 acaatgcatt tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg
     6661 gataatcctg agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga
     6721 ctagtaacgg atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct
     6781 atcggtattc ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa
     6841 ccgatctttt gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag
     6901 ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac
     6961 gggattcttt taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc
     7021 ttccgctcag gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa
     7081 ataaagaaat gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca
     7141 atcctatcaa aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg
     7201 gggaatcaaa ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta
     7261 tatcgggaat ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga
     7321 aggaatctat ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa
     7381 aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca
     7441 agatttagcc tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcgaaaac
     7501 cattgaagaa gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa
     7561 aaaagctgcc ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt
     7621 tgttgggagg ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac
     7681 cgtttatttg aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg
     7741 ttttaatagc tcttttttat atagatagtg cccataccta tgaataataa aacgatactc
     7801 catataccaa gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag
     7861 aaaatatctc ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga
     7921 ctgattgatt ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa
     7981 agaagtgtac ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg
     8041 attgtactta aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca
     8101 tacatcggaa ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct
     8161 cccaaaccat ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt
     8221 agcatagctg caccataata aatggtaaaa cggatgctat gtttctttac catactatcc
     8281 ggtaatagat tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa
     8341 agaaacaagg cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt
     8401 tcgtagtaat ttgctaaagg gagatgcgct ggcaagtcaa aaatagaata tctttggatc
     8461 tgatgttctt tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt
     8521 ccttgtcttt gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct
     8581 tcgaccattt gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg
     8641 ttgcttttct ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca
     8701 atcaaacgtt gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt
     8761 ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag
     8821 tttactttgt tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct
     8881 catataattt acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt
     8941 ctccattttt tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg
     9001 aagaaagaag aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat
     9061 caatacttcc tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac
     9121 tgatcaagta agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg
     9181 gcaaagtaag gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc
     9241 tctgccaata gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact
     9301 gattcgttga ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga
     9361 tgttgtgaag ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca
     9421 aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta
     9481 aaagattcaa ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca
     9541 gtaatgtcat agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc
     9601 taaattaaat gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt
     9661 ttatttaaaa ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac
     9721 ctttggcaca aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa
     9781 ttagagtatc aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac
     9841 atttcgctaa aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca
     9901 aatattggag cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat
     9961 ttaccaaatg aggctttggc taccatggta agcccaatgc taacttatct gattccttta
    10021 ttgatcggtt atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca
    10081 attgcaacga tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc
    10141 atgggacctc ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc
    10201 aaatccggtt ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta
    10261 gcgttattag gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca
    10321 aaaggtgtag agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa
    10381 ccagcaaaaa tcttattttt gaataatgca atcaatcatg ggattttgac accattggga
    10441 actgaacaag tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt
    10501 ccaggacttg gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca
    10561 gcaccaggag ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac
    10621 gttatgatga aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc
    10681 attttccaat tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg
    10741 attctagcga tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc
    10801 gcagctgttt cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat
    10861 gaagacttcg aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc
    10921 caaggtattc ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat
    10981 gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca
    11041 gacttgcctc aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca
    11101 ttgatcgtta ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt
    11161 tttgtctcag tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta
    11221 agcagcaagc agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt
    11281 tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca
    11341 gcaacaatgg ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg
    11401 ttaagaaaag tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa
    11461 gaaagtttag cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac
    11521 agcatggtca atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa
    11581 gggaggaaaa cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta
    11641 aaaccaaaga agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg
    11701 atgaggaata tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga
    11761 attttatcgc gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca
    11821 tctgtgtcgt tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg
    11881 cgactgtttt gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa
    11941 tcgcacttta ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag
    12001 aagaagtaac ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt
    12061 ttggtgcagg aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg
    12121 agattacttt cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat
    12181 acgaaatcga attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa
    12241 tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta
    12301 ctaccgccat tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc
    12361 aagaaagaga aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga
    12421 ttggcggttc agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta
    12481 ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc
    12541 atgaagatcc tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc
    12601 aaagaaaaaa taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat
    12661 atatcgaacg caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg
    12721 ccttgttagg ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat
    12781 taaaatcagt tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag
    12841 agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg
    12901 atgcgatcac acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta
    12961 cacgtccagt gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa
    13021 tggggattat ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa
    13081 tgaagatcca tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga
    13141 aaacaatcgg gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata
    13201 ggatacgaaa aaggggaatg tgacgaaatt tt
//
LOCUS       DACDXE010000085        13093 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.085, whole genome shotgun sequence.
ACCESSION   DACDXE010000085 DACDXE010000000
VERSION     DACDXE010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.085"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(108..731)
                     /locus_tag="IWM23_002452"
     CDS             complement(108..731)
                     /locus_tag="IWM23_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885052.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(890..2287)
                     /locus_tag="IWM23_002453"
     CDS             complement(890..2287)
                     /locus_tag="IWM23_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9885053.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2319..3290)
                     /locus_tag="IWM23_002454"
     CDS             complement(2319..3290)
                     /locus_tag="IWM23_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9885054.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3664..4089
                     /locus_tag="IWM23_002455"
     CDS             3664..4089
                     /locus_tag="IWM23_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885055.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4577..5278)
                     /locus_tag="IWM23_002456"
     CDS             complement(4577..5278)
                     /locus_tag="IWM23_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAP9885056.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5294..5734)
                     /locus_tag="IWM23_002457"
     CDS             complement(5294..5734)
                     /locus_tag="IWM23_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9885057.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5734..6309)
                     /locus_tag="IWM23_002458"
     CDS             complement(5734..6309)
                     /locus_tag="IWM23_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAP9885058.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7157..7636)
                     /locus_tag="IWM23_002459"
                     /pseudo
     CDS             complement(7157..7636)
                     /locus_tag="IWM23_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7795..8496)
                     /locus_tag="IWM23_002460"
     CDS             complement(7795..8496)
                     /locus_tag="IWM23_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9885059.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8604..10013)
                     /locus_tag="IWM23_002461"
     CDS             complement(8604..10013)
                     /locus_tag="IWM23_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAP9885060.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(10061..11926)
                     /locus_tag="IWM23_002462"
     CDS             complement(10061..11926)
                     /locus_tag="IWM23_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9885061.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(12047..12898)
                     /locus_tag="IWM23_002463"
     CDS             complement(12047..12898)
                     /locus_tag="IWM23_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9885062.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3752 a         2818 c         2133 g         4390 t
ORIGIN      
        1 agctgacctt cggtattagc tcaaaaaacg gcaaaatatg aggaactatt ttgccgtttg
       61 tttctctaat actcaggtca aaacgctgat ttcacaacct ctgtttttca ttgtccatag
      121 gattgatatt gtccttgtga tgactcattc gtaataattg aggcttgatt ttcttcactt
      181 cccgttatcg tttgtccact gccagaatct gaaggacttg ttcctgtcga ctcgtttttt
      241 tgagaagaga cagttgtttt cttgacttgt tcatttgtct ttttccattc cgctaaacga
      301 tcggcgacca cttttttgtc tactttttca gaataagcgg tgatcacttt tccatttttc
      361 aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa cggtcaaatg cttgtcttta
      421 aattgatatt gtctgagaga aatcgcacta agtacgtcat cgatattaat aaattgattt
      481 tcttcttttc cttttggcaa ataaatgtcc aattcgcctt tttcgtaatt ggcagttatt
      541 ttatactccg cccaccaata agttgagaat tcttttttga aattatctac tgattctttg
      601 cttgctacga ttttttctgt ttctttctga gcaagatctg ctgtcatttt tcctgaaaat
      661 gctgcggata aaataagcag taagaaaaaa ccgaataacc aaaagagaag aaacggtctt
      721 tttttcctca tgctttcctc cactcctatt taattttgac tattctatta tatacaaaaa
      781 atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat aattgaactt ctttttcaca
      841 aaatggtaag aatggagaat ttcacgcatt tgctctcaac tattctttct taagcctcaa
      901 atccgttttt atctgatacg tctttgaacc aatgacccga ttttttgatt gttctttttc
      961 catcttgtgc taagtctact gaaataaagc catatcgatt cttatatgca ttggtccacg
     1021 accagttatc catacatgtc cacatatgat agcctaaaca attggcacct tcttgaatgg
     1081 cttgatggac atatttcaga tgagattgga caaattcgat ccgataatca tcgtggatca
     1141 tgccatcttc tttgataaat cgtgcttcgt tttctacacc cataccgttt tcagaaataa
     1201 agcagtcgat atttccataa tgatctcgta cattgatcaa aatatcatag attccttgtt
     1261 catagatctc ccatccgcga taaggattca ttttacgacc aggcatttca taattgtcga
     1321 aataatcctc aggcattgga ccgtttttag tatcgattgg tgtttctttt gcttttaccc
     1381 ggcgtggctg gtaataattg atacctaaaa gatctactgt attttctttg ataaccgtca
     1441 aatcctctgc atggatcaca ggcatgagat caagttcttt tactatctca atcaattcac
     1501 ttggaaactc gccttttaca gctggatcaa ggaatgaacg attgaagaac gcatctgcta
     1561 tttgcgctgc tttaacatct tcagggtttg attcgtctct tggatagctt ggtgtcagat
     1621 tcaagatgat accaataaca ccaccttgct ccatttcatg gtaagatttg atggctaacg
     1681 ctgaagccaa cgcttcatga tagccaactt ggatcgcttt tttcatatct gactcttctg
     1741 gataatgcca tcgatataaa taacctgctt cgactggaac gattggttca ttatgagtaa
     1801 accatttttt tactttcgaa ccaaacaatt caaaacaact tttggcatat ttttcatagg
     1861 cttcaaccgt ttctcggttc aaccatccgc ctttttcttg cagatacatc ggcatatcga
     1921 aatgatacag attcacaaag ggttcgatac cgttttcaat caactcatca aaataacgat
     1981 gatagaaacg aacggcttct tcgttgattg ttccagattc atcaggtatc aaacggctcc
     2041 attggataga tgttcgaaat gaattatgcc cagtctgttt catcaactgt acatcctctt
     2101 gatacttcgt ataaacttgc gaggtttttt caggtcctac ttgatcaaag aacttttgcg
     2161 gttctttttt gtaccaataa tcaaaaatgt tctctccttt tccatcgcct gaaatccttc
     2221 cttctgtttg tgggccactt gctgcagaac cccaccaaaa atttgctgga aattgatatt
     2281 ttttcatcaa agcacctctt tttctttttt agttcgtttt agttgattcc atgtatgata
     2341 ttttccatca caatcacttt ttcattttct tcctcttctt ttttgattcg ttttaacagc
     2401 tgttctgctg ctcgttcccc gattttatct gttggtggcg tgatcgtgat tttcggaagg
     2461 atactcagat cgtttttatc taaataatca tagtgactga acgcaatatc cttcccgatt
     2521 ctgacacctt cttgattgaa acattcgatg gcaccaatcg acatattgta attgctgaca
     2581 aaaatagcag ttgccccttt ttctaataat gcttttgccc cgtaatagcc gctttttcta
     2641 gaatagtctc ctgtaaagac ctctaattgg atcgatggat cattttggac cgcttctttg
     2701 gctcctgcta aacgttctct tgctacgtag tctgtttgag tggcagcaat aaatccgatt
     2761 tttttatgtc cttgttcacc atgatgagcg atcacttttg ctacactttc tcgatcattc
     2821 actaatattg tatcaatacc tgctggtcct tctggacaat tcaatgcgat aactggaata
     2881 ctcatttttg tgagcatttc aaatccagac cactcttctt cggataagaa gacaatcagc
     2941 ccatcgatca catgcttcat caggtattca attttttgtt caattttttc tggctgatcg
     3001 gcaaacccgc ttaataataa gctataccca ttttcttcag ctactctttc gatccctgag
     3061 acaacttccg acccaaaacg gctggaaatc ccgtttatca aaagtccaat agagaagctt
     3121 cgattattct tcaacccttt agcaatgatg ttttctttat agtccaacgc tgaaatcgct
     3181 tctgctattt ttttcatgtt cttttccttc agttgctgac cattcaagta acgggaaact
     3241 gttcccactg aaacaccagc catcttagcc acatcacgga tcgatgccat acattctcac
     3301 tcctgccagt tacatttgat ttctctttct tgttgttcga ttatgatgac cgcattcttt
     3361 gcatagtgtt gcttgtcatc aatatccata taactaaagt cttcagtaga gcaacttcct
     3421 ctcactatta attatactta aacaagtatt tgaaccggtt catttttaat atagcatttt
     3481 tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa gtggcggatt ttttgccttt
     3541 ttaaaatttt attctaaaat ttactctatt tgtgggtgtt ttgtgcgttt ttctcttcca
     3601 tccgtttgaa gttttacatt ccaatggtat aatttcttta tcctatcaaa tgggggaagt
     3661 attttgaaga aattaatcgt actatttatt ttgtctgcgg gacttttgac attgactggc
     3721 tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa caaccgattc ttctgtctta
     3781 aaagcggaaa aagcagatac caccaacagt gtatatacgc taaaagtacg taaagatatg
     3841 gatttatccg cgggacagtt gacatttaca gataaaaaaa tggaatggac acgcacgtat
     3901 cagacacaag gaactgattc ttctgaatca tctgaagaaa aaacagtgct gacagatatc
     3961 aagatcacta ccaaagatga cgaatatatt atttcaggaa aagaaaataa aaaagaaaca
     4021 acagtcatat ttacaaaaat cggtaattac cgtttgaagg atgaggatgg caacatctac
     4081 agtctatagt gccgtctgtt ggatcttctt ttgtttctcc ccttcttttc tgctatagta
     4141 aagaagcaag atgattgtaa aggagacgaa atggatgatc gatagtcctg attattgtac
     4201 atgtaataac aacaaaaata tccatacaga atttaatgac caaggaagct tgagcatatg
     4261 cgacaactgt cacaaactag tcaaagattc atttaaaaca tttgaatctt atcctgttca
     4321 aaattatgta aaaagtagaa gaagataaac aaatccaagc aaaactcact atggctgatt
     4381 tttatgaact tcaaactcag cttcttcctt tgatcaagct tcatcagtaa aacaaacagt
     4441 ggatcatttc agtttacaaa tcaaagagta aagatttatt tatttcaaat atttgaatat
     4501 aaaataacca aacgatagaa aggcttattc tacatcacgt agtaattttt ctttcccgtc
     4561 gtttggtttt ttatttttag cctaattctt gaccttttgc atgctggtta tccgtaaaat
     4621 attttccagc aggctttcct tccaattcag ctaatttttc ttggatctga cggatcacct
     4681 tttttgcatc ttctggattt tctacaaaat cgaatttatc cccatccact tgtattttag
     4741 gacatacatc aaaatcttca taccattgat tatagcgggt gttcaacgtt tcataatagg
     4801 tatataacgt ctcatcgctt tctagctgtt catagtcacg cccacgtttt ttgatacgtt
     4861 ctagcatcgt gtccaaagaa actttgatat ggaccatcaa atcaggccgt ttttttggag
     4921 caacatcatc taactcgttt agcatcgtat ctaataagct atcgtactgc tgtacttcga
     4981 tatccgttac ccgacctaaa tcagcgttta gatggaacaa caggctatct tcatagatgg
     5041 atcggtctag cacattgtca tcgttcacta aagcattttt cattgctaaa aatcgcttgt
     5101 tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg atttgaataa aagagcggca
     5161 atacttcgtt atcttcgaca ttttcataaa atggtttaga atcaatctct tttgcaagca
     5221 tatccgtcaa actgctctta cctgcaccaa tcgttcctga aatcaaaatc actgacatac
     5281 aacccttcct cttctataag tactcgccta cgtaagtttc aacaggcaat gtttcaaaat
     5341 catattcacg tacagcttgg tctgatttta aatacgtatg gagactttgt gtaatcatca
     5401 agttcgtagg taccgtctct tcttgtacaa tgatcatcgg ttttttcaac atagttgcca
     5461 cacctaattc gtaagccgtt cctggatcga ttgtttgatg ctcaaagtca aggatagcaa
     5521 caacaaattc tgcatttgtc agttcttcca tatctttttc gtatacttcc tgcgcccatc
     5581 cagcgctgaa tagttcataa ttgctttctt ggtgatgacg tggagagtag aaacttgtga
     5641 ctgttttgtt ttcctccaac gctttttcga tacgacttac tcgatcgatt tgctcctctg
     5701 aaaaaaatgg tcctgctaaa tatatttgac tcattatttt gcctccttca ataataactg
     5761 atttttaata gatactgctc gtaattcatt gaccagttcc aatggattgt tggaaaagat
     5821 tacttgatcc aaccattttt tatccataaa atcctctttt gcaaaagttt ctaataattg
     5881 ttgcaagccg ctgtaataac cgccaatatt caatattccg attggcttgt cgggcgtcag
     5941 accgattgct atccaagaaa gaagttgtga gaattcctcc aatgtaccta gacctccagg
     6001 gaaaacgagg acagcatctc ccttttcaaa taataatacc tttctttcat cgatcgtttc
     6061 tgtaggaata aatgtagtaa catcttttcc aggcaattca gtttcaaaaa gcccattcgg
     6121 atagatacct gtgacttttc cgttattttc cagtactgtt ttactaatca ctcccataat
     6181 cccggagtat gaaccaccgt aaaccagttc gatttcttct tgtgccatgt attttcctaa
     6241 cgtttgcgca ataaatttgt aactttcttt attaccaaaa cgggaaccgc aaaaaacagt
     6301 aagtttcata aaaaaacact ccctaactca aaagtctatc ttcattctac acgtgttttt
     6361 ttaaaataaa agactagatt ttgtagtttt ttagtggtat ataggataga cgccactata
     6421 tatagtattt agaaaaaaat catttttcag ccattttata gataaaacaa aaataaataa
     6481 cgtgttttca tcgatatgaa atgacttact tatagaagat actgggattt cgtcctatat
     6541 gccaataacg tgtattattt taaacaacaa atacttgttt cttttattaa cgaaaaaatc
     6601 attttgttct cgtgttatgt ttcactgata aatgatgttt ctctaaataa aaaagccgac
     6661 gaactaaacg atcaaaattt gatcattttt gttcatcagc ttctcctaaa aatctttttg
     6721 ttaacgataa atagctggat ttgtaaaaat atcacttgaa tcgtcactgc tggaagaaaa
     6781 ttcagtgata accgcacctg ttgcgcctgc ctggaactag tgtttgatat tgggctcgaa
     6841 cgtgtactga tctctagcat gaagaataat ttcttttttt actgtataat aactttcttt
     6901 cctttttgga atagtacagg aaagtttttt tgtttccttt ccttcgacat aaggtatacc
     6961 agaccctttc gacaacgaaa ggttctcatt tttccttcat cgtcatttca aggagggtgc
     7021 cgatgctctg gacagtttcg atcgctccta aaccaaaatc tgtatattcg attctttcta
     7081 actctgattc tttcatacca ttcctgtctc aaggtacatc tattgtactt ttttcttgta
     7141 atccattctg cctctcctaa ctaattgtca aatcatccca agatatgtct tcttcctctt
     7201 tttcttcttg gatgatttct gcattcggat ctactggttt tttcaataat aaaatggtta
     7261 ttcctgtgac aaaagaacca gctaacaatg caccaatgaa tgcccatggt ctggtcatcg
     7321 tagcgactgc aaacattccc gatgccggaa tacggctatc tgcgccccaa aacatgctta
     7381 atccgccacc tactgtccca ccgactcccg ttgctatgac attgcgtgcc aagtcgttca
     7441 atacgatagg gaaacatccc tctgtgatct gacagatccc cataggaaaa gcggtcttca
     7501 atgtatcgat ttcttgacga ttgagtatat ttttacgaat gattttccct aagaaataag
     7561 cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat caatacagtg attggttcat
     7621 agattccttc tgaaaatcgt gccacacccc cttatccaga ataaatggta atccagaaac
     7681 agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa tatgagaagc tatttttgtt
     7741 tggctgttct tattacttga tgcagggcag ctttttcaca acctcttttt ttatttatgt
     7801 ttcataatca atatggtaag gatgcagctc cgtatggtag accgcctccg aatattcaat
     7861 catttcccca atttgcgtag acgatttccg tatccgtttg atgccgatct gtgcgtctgt
     7921 tttcagaatt ccttgttctt cttctgttaa aaatactgcc gagaaatcat cagaaaatga
     7981 gtcaatagta tatccttttt tcgtcagcaa acgatacaaa gattctttgc caaattcttc
     8041 tctgctgaat tgcatcattt cttgtggaag ataatgaaca aaataaatat aaggctgatt
     8101 gttcaaataa tataagcgct taaaacaaac agcttgctca ccgaaaaagg aaaacaaagg
     8161 atcttccttc tttatatgga ccaattgcaa atccagcgtt tctttccgga cttcatatcg
     8221 ggagttctcc aagatttgcg taaagcttgc cgctttggat aatcgattgt aaggacgatt
     8281 gcttagaacc gtcgtcccac gaccactttt ctttgcgaca tattcatctg atgccagcag
     8341 ttcgattgcc tttcggacag tgattttgct gacttgaaac agttcttcta attcactttc
     8401 tgtcggcaac atcgaaccga ctggatattt tcctgagaaa atatcttctt taattttatc
     8461 tgcgacttcc aagtatagta cacgtgtcct tttcataacg tcaccctttg ctttactatt
     8521 actaaaagaa tagcacataa aaaataaaaa aagaacacaa atcacatgtg agaagtgtgt
     8581 tctttttcat tcagctgctt taattacaag tttacgccat ttgaggcaat tacttcttta
     8641 taccaatcaa aagatttttt ctttgatcga ttcagtgtcc catttccttc atcgtctaga
     8701 tccacataga taaagccgta acgtttactc atttctcctg ttgatgcact gacaagatcg
     8761 atacatcccc aaggcgtata gcccatcaaa tctacaccgt ctgctactgc attagccatc
     8821 gcttcgatat gacgacgtaa ataatcgatc cgataatcat caataatcgt gccatcctct
     8881 tccatatgat caatggctcc aagaccgttt tcaacaatga ataatggttt ctggtaacga
     8941 ttgtataatt cattcaatgc gattctcagc ccttctggat cgatctgcca gccccattca
     9001 cttgcctcaa gaaacggatt ttttactcct cccagcaagt tcccttctga agttgctgct
     9061 tgtggatcag tctcattgat tgccgtagac atataataac taaatccgat gtagtccact
     9121 ggatattctt ttagcaactg gaagtctttt tccgtttgct ctaatttttc cggatcgact
     9181 ccatattttt tatgggtacg cgctgtgtat gcagggtatt ctccacgtac ttggacatcc
     9241 gtgcagaaaa aattgaattc ttggttttgg atcattgttg ctgcctgatt gactggattg
     9301 gcatcgatgc tgtaagttgt tgcatagata atcatgcagc cgatttggat atccgaacgc
     9361 aattcgtggc cgatttttac ggctttgcta cttgcaacga attgattatg ccatgcttgg
     9421 aaaatatttt ggtactctcc tgctccattg ctttttacta atccttgact taaagctgga
     9481 aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat atttgacttt actgctgtat
     9541 ctctctaata ctgtctgagc aaatcgttca tagaagtcga tcagttttcg atttttccag
     9601 ccaccgtatt cttttgccaa atgaagaggc atttcgtaat gagaaatcgt gatcacaggt
     9661 tcaatatcat attttaagca ctcatcaatc agctgatcat aaaattctaa tcccgcttcg
     9721 ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc aggcaatcga aaaacgataa
     9781 catttgaatc ccatttccgc aaacaaggca agatcttctt tgaaacgatc gtaatgatcg
     9841 attcctcgat gattcgggta aatatatttt tcttgatcaa tcgtccagtc aaattcttca
     9901 ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag catctgcaac ggatagtcct
     9961 tttccgtctc gatcataagc cccttctaat tgatttggca gcagttgctc caccccataa
    10021 aaatttttct gaaaaacgtg attgcatcct gatttcctcc ttattttaca attgccagaa
    10081 catcttcgtt tgcaataatc tctttttggt ctaattcaag tatatcggca aatgcatcag
    10141 tattcgtaat gacaactgga gtgattacgc tatagccagc tgctctaatt gcttcgatgt
    10201 caaaacgaat caataaatct cctgctttga cgagatcccc ttgtttgact aagatctcaa
    10261 agccattccc atttaattct actgtatcca tcccgatatg aatcaaaatc tccgctccgt
    10321 cagtcgttgt gatccctaca gcatgtccag ttggaaaaat agtcgtgatc tctccgtctg
    10381 caggtgcaac tagttctcct acttctggat caatagcgat accttttccc atcacgccag
    10441 aagaaaaaac ttggtctggt acttcactca atggcagtac tttacccgtt aaaggacttt
    10501 ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt cgcatgtttg ttttctaatt
    10561 gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt acctacgaat gcaagaacaa
    10621 aagcaattcc tgtagctatt actgctactg tcacatttga ctctacacca tccaccgtat
    10681 taatgaaagt cggaatcgtc aataagctga ccaaactgct ggagaaagct tttactccgg
    10741 aaaatccgat aatagctcca ccaatacctc cagaaataca agcggcaatg aatggtttct
    10801 tcaaaggtaa tgtcacacca tacacagttg gttcagtgat tccaaataac gaagtcactg
    10861 tagaagaaat ccctaatgct cgaagtttcg tatcttttgt tcgcaatgct acagctaaag
    10921 cagccccacc ttgtgccaag atggctggaa gaagcattgg catcaataca tcaaacccat
    10981 attgttcgat attcaaaaac atgatcggta cgaatcccca atgcatgccg aacatcacga
    11041 atacttgcca taaacttccc atgattagac ctgctacgat tggactaaat ccataaatcg
    11101 agttgaagag accgcctaaa gcattaccgg caaccgtccc gattggtcct aaagcaagaa
    11161 atgttaatgg aaccatcact actaaaacga caagcggaac taaaatcatt tgcagaaatt
    11221 gcggaatcac tttttttacg aatggctcta atttactttg aacccatacg gctagaatga
    11281 ttggtatgac agaagaagta taaccagatg gtgaaagaat cactgggatc ccaaagaaac
    11341 tgatagcttc tcccgcacca gcaactgctg tgatagctgg atacacaagt gccatagcaa
    11401 ttgctactgc taaaaactgg ttggctttga actttttagc tgctgtaaaa gcaagcatga
    11461 caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa tatttgataa gtaccgctat
    11521 cagctgtcag ccagcccatc accgttgcca atgataagaa tcctttcaat accccggcgg
    11581 ctgccatcgc ccctagaaaa ggtgtaaata tactagagat aatatcgatc agacgattca
    11641 aaatattttt ggactcttct tttccatcct ccgaaactgc aagaccttcc tcatccacga
    11701 tcgattgata aacatctgct acattactgc caatcactac ttgatactga ccgccacttt
    11761 gtacgacttg gatcacgtcc gggtcttctt ttaacttttg cgtatctgct aaagattcat
    11821 cttttaatct gaatcgcaac cgcgtagcac aatgtactaa acttttgaca ttcttttgtc
    11881 ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt atccattgtt ttttccttct
    11941 ttctttttta tttaaaaaat aaaaacccag attgaaaaaa acagtcacaa acattcgtga
    12001 acctattttt tcaatccagg ttttgcctgc catacagtaa caatccttat gattggatgc
    12061 tttttttctc acttactact ctttgaatat gaatcgttaa ataaatctgc tctgcgatcc
    12121 ccatttcctg tttttgcgtt tgtcgaagat actcattgat ccgttcgaca caatgaaacg
    12181 cttttggata ctttccttga actaatgcgt agagaaattc atcttgctcg tcagatcctt
    12241 cccctttgag ataacgctgg gcaaaaaact gcagatgagt aatgatacgc tgataattca
    12301 aagattcttc atcgaacacg atgccgaaga atttactgat gatactcagt atatctctta
    12361 ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt tccttgttgg ctattcacaa
    12421 aatgaaaggc aataaaaccg gcttcatcct cagaaagttc cacacccgtc ttttgtttca
    12481 cgaacaaaag agcctctttc gctgcttgat attctttcgg ataaaatttt tttgtttcaa
    12541 ataataatgg attaggcagt tggatatttt cttttgctcg tgtgatcgca taatgaagat
    12601 gatcggtcaa tgtcagataa atattaggcg aaaattcaac ttttagttta tcttcagcca
    12661 gttcgactac ttgtttgacc cattcgatct cctctgccgg aatatcagcc atcagctgct
    12721 ggaattggtt tttagcaaaa gaatctctta aaacaaattc tttttggacc gcttctggcg
    12781 aaatctcgtc cccaatcttt ttttgaaaag ctagtccttt tcccatacaa acgacttctt
    12841 ccccgaatcc atttttcgaa atgacgacat tgttattcaa aattttatca attttcattt
    12901 tgcttcgccc tctcaagaaa aataaaaaaa cctagaattc gcctactgtt tcttagctga
    12961 attctggttt tgcctgccgt acagtaacaa tccatttctt attcaaaatc atcataccat
    13021 cgaattttta tttatgcaag ccttttcata taaaaaataa aaaaactgga acacaagtct
    13081 atctagaaaa caa
//
LOCUS       DACDXE010000086        12990 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.086, whole genome shotgun sequence.
ACCESSION   DACDXE010000086 DACDXE010000000
VERSION     DACDXE010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.086"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..171
                     /locus_tag="IWM23_002464"
     CDS             <1..171
                     /locus_tag="IWM23_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885063.1"
                     /translation="TYEEGITNAMIYPYTNGKIEAKNTHIKTMKRVSYGFKSFENMRI
                     RIFLINQLIKVR"
     gene            complement(341..520)
                     /gene="rpmF"
                     /locus_tag="IWM23_002465"
     CDS             complement(341..520)
                     /gene="rpmF"
                     /locus_tag="IWM23_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAP9885064.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(578..1147)
                     /locus_tag="IWM23_002466"
     CDS             complement(578..1147)
                     /locus_tag="IWM23_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAP9885065.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1333..2235
                     /locus_tag="IWM23_002467"
     CDS             1333..2235
                     /locus_tag="IWM23_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAP9885066.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2270..4054)
                     /gene="pyk"
                     /locus_tag="IWM23_002468"
     CDS             complement(2270..4054)
                     /gene="pyk"
                     /locus_tag="IWM23_002468"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAP9885067.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(4128..5090)
                     /gene="pfkA"
                     /locus_tag="IWM23_002469"
     CDS             complement(4128..5090)
                     /gene="pfkA"
                     /locus_tag="IWM23_002469"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAP9885068.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5258..8572)
                     /gene="dnaE"
                     /locus_tag="IWM23_002470"
     CDS             complement(5258..8572)
                     /gene="dnaE"
                     /locus_tag="IWM23_002470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAP9885069.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8803..8988
                     /locus_tag="IWM23_002471"
     CDS             8803..8988
                     /locus_tag="IWM23_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAP9885070.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(9050..10246)
                     /locus_tag="IWM23_002472"
     CDS             complement(9050..10246)
                     /locus_tag="IWM23_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAP9885071.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10416..11852)
                     /locus_tag="IWM23_002473"
     CDS             complement(10416..11852)
                     /locus_tag="IWM23_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAP9885072.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            12145..12954
                     /locus_tag="IWM23_002474"
     CDS             12145..12954
                     /locus_tag="IWM23_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9885073.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3905 a         2724 c         2290 g         4071 t
ORIGIN      
        1 acctatgaag aaggcatcac caacgcgatg atctatcctt acaccaatgg aaaaatagaa
       61 gcgaagaata cccatataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
      121 aacatgagaa ttagaatctt tttgatcaat caattaatca aagtaagata acaaaaaatc
      181 tgagccagaa tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta
      241 ttgacaaaga gcccatattt tttatttacc gactacaaaa aaataaacct caaggctcca
      301 tggttagcct tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta
      361 cgtccttacc atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac
      421 cacagtttgg acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg
      481 cttttttagc ttttgatgtt cttctagcta gtactgccat tttgctacac ctccttgtaa
      541 agtcttgtat ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg
      601 atttagaatc aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt
      661 cttccatttt tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg
      721 gaagttctgt tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga
      781 tgttgtcttc aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat
      841 ctgcagacat ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta
      901 cagaaaaatc catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag
      961 tcgtctgcac agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga
     1021 cttccactgg cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt
     1081 cgagcgtctc atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc
     1141 atttcatttt tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg
     1201 tcaatgaaat tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa
     1261 agcctctgtt tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag
     1321 cggatgatct tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa
     1381 aattttacga aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta
     1441 caacgtcttg aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa
     1501 ttaatcgtca cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag
     1561 atttctactg caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg
     1621 cctcctatta tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa
     1681 ttgatgaatc aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga
     1741 caagggagga tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta
     1801 gaaacagagt tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt
     1861 tccaagcagg atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac
     1921 gaacattatg tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca
     1981 aaagttgttt atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg
     2041 ggtatcggtt ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct
     2101 ctttcagatg aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc
     2161 gtcacgcctc ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa
     2221 aacagctctt cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg
     2281 ttgtttcgcc acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg
     2341 taattgttgt tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta
     2401 caactgcagc gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga
     2461 ttgcaggcat atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt
     2521 tttttacagc ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc
     2581 caacgccttg agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg
     2641 ttccgcgttc accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga
     2701 aacctaattc aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt
     2761 tttcagcaac tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt
     2821 caaaagtaac agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt
     2881 atcctgattc agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta
     2941 cagataaacc gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac
     3001 ctaattctga taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt
     3061 caactgggta gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa
     3121 atacagcgtt agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg
     3181 attctaacat ttgtgtagca gtgataactg ggataccagc agcattacat tttttgataa
     3241 tgcgtttttg aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg
     3301 caatcataac accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag
     3361 attcgatttt agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga
     3421 tatctagaac gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt
     3481 ctaaaccaaa acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa
     3541 cgcctggtgc atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga
     3601 ctaattcacg gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa
     3661 ataaaatgtg tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt
     3721 tttcttttga acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa
     3781 atttgatgta tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga
     3841 tttcagctcc ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt
     3901 cacggaccat agtcatacgt gataattgtt cttcatggtc accatgagaa aaattaaagc
     3961 ggaagacatt tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg
     4021 gtcctaatgt actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg
     4081 tttatttaaa gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt
     4141 gaattattta gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca
     4201 atgatgtcag acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct
     4261 tcttgaagca attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg
     4321 cttggtgctc caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcacca
     4381 aattctgaaa gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc
     4441 aagcaatgtt tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata
     4501 tcaaaatcat gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa
     4561 gcgatgtctc ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc
     4621 gctgtgtcac gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg
     4681 attgtgaaat cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg
     4741 aaaccgcgtt ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact
     4801 agaccttcga ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt
     4861 gcaaattctg gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct
     4921 acatctgcca catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg
     4981 atcccgtata cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca
     5041 ttcattccag gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt
     5101 cacctcatta aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa
     5161 ccacgactaa cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg
     5221 aaaaaaaatg aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt
     5281 cgccaagcag ataaataagt tgccccttta tactcttgct gtcatccacc caataagcat
     5341 ctgacagaac tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg
     5401 ggtgtttctg aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg
     5461 taatacgcaa atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa
     5521 agctttctcc aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa
     5581 caaagatcac tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa
     5641 ctgtgacaga aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt
     5701 tttttgtccg aatttctcta acttccttga tcatcactaa taaattgact ggttgattag
     5761 ggacgatatc tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg
     5821 caggatgacc tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta
     5881 gtgtatagtc agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc
     5941 caccactgta aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt
     6001 taggagataa ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca
     6061 gccaacgctt atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt
     6121 ttctttcgtt taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc
     6181 cgaatctgat ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg
     6241 gcgaggaaag tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg
     6301 tgttatgccg aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta
     6361 gatacgccat ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat
     6421 agtttgcaaa tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct
     6481 gttgtaatgc tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt
     6541 tactcactgc ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg
     6601 cgatctgcat gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga
     6661 tcagtgaatc atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat
     6721 ctatattttg catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt
     6781 tttctggatc taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc
     6841 ctgaggtttc gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt
     6901 ttaatcggat ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg
     6961 ataagttgcg tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca
     7021 tcgtatactg ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta
     7081 gattcttatc gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt
     7141 ctaaaatcac tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta
     7201 ctaattgatg cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg
     7261 cttttgacca gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa
     7321 gaaccatttt tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat
     7381 acttttctcg aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga
     7441 tatctggcat cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta
     7501 atggatccac atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac
     7561 gacctgcacc ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca
     7621 agaaataatc atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt
     7681 ggtattcctt tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta
     7741 aatattctcc agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga
     7801 actggaattc aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa
     7861 gacccgcttt ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg
     7921 ttagtggtaa agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt
     7981 tcatcacttc aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac
     8041 aaagcgaata attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt
     8101 tttctgtcaa gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat
     8161 gaccgaaaaa gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa
     8221 caaacgctag atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt
     8281 ttcggctgga aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca
     8341 agccataatt tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc
     8401 cttcttctac acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta
     8461 tggctaatga agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg
     8521 tactttgtaa aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt
     8581 ccgccttttt tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt
     8641 aagatagcag gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga
     8701 atgagttctt tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca
     8761 aagaaaatct ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca
     8821 ttattctggg ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc
     8881 ggaacatatg attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt
     8941 ttgattggtg cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa
     9001 gagcttgaga tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc
     9061 tgcgaagaac tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac
     9121 ttgtcacaat aaaaacagca ctgtaattat aagcacgcgc gactgtcgat cctagcatgg
     9181 gacctaaaac ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc
     9241 cttcttgagg cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg
     9301 aaaagcctaa aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag
     9361 gtaaataaca gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct
     9421 tatcccctag ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa
     9481 caatcaagcc gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata
     9541 aggtcaaaat agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga
     9601 gaccgatcaa aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat
     9661 cttttttctc gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga
     9721 tcatcaacaa agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg
     9781 caccgcccat tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta
     9841 acgcccatcc gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa
     9901 tatatccaga caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta
     9961 caaaagccaa agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt
    10021 ttcggccttt tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag
    10081 cagtcacaga gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt
    10141 gctcaacata aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac
    10201 agccaatcca cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt
    10261 tcgtctcttt tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga
    10321 gaaaaatccc gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga
    10381 tcagaacaaa tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag
    10441 ttgataatga ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct
    10501 ttgattactt tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta
    10561 tagcacaatc ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc
    10621 catttgatgt ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca
    10681 gttgcttgat ttggtacaat ggcgatcagt ggagagaata aactacttaa cgcgaatagc
    10741 actgccacta ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat
    10801 tcgacataag ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca
    10861 tcggcaaata gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc
    10921 tcgtcttctt ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta
    10981 tcgaaagtat cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa
    11041 gaatcactga ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct
    11101 aattctttaa atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca
    11161 acacccataa aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg
    11221 acatttttta caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga
    11281 gcgttgtcaa agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct
    11341 tttccgcctt caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat
    11401 cctgcatttt tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt
    11461 tgaagacttt cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa
    11521 atattgatta atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc
    11581 ccaaaaacga ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat
    11641 ggtacatttg cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct
    11701 agaaatactg cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag
    11761 atataactca tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta
    11821 ttgtttttct ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc
    11881 tgatggaaaa ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc
    11941 aaattatata gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa
    12001 tacttaacgt tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt
    12061 gttaggaata gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc
    12121 tagttgagga aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga
    12181 actaccctca atcaagattc gatgattaca tctaaaacaa aagagactct aaaaaaagca
    12241 atcaatgccg gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag
    12301 ttttaccaac aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac
    12361 atccctaatc agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt
    12421 gaaatcttat cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat
    12481 acttttttta ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca
    12541 ggagagaaga atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc
    12601 cgaacagaca aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt
    12661 tatgtcgatg ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga
    12721 atccaaaaag caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac
    12781 gtcattgcct ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga
    12841 gtagctatgg ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg
    12901 agcaatcagg aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga
    12961 attagattcc acaaaaaaga ggttgtgaca
//
LOCUS       DACDXE010000087        12670 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.087, whole genome shotgun sequence.
ACCESSION   DACDXE010000087 DACDXE010000000
VERSION     DACDXE010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.087"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..305
                     /locus_tag="IWM23_002475"
     CDS             6..305
                     /locus_tag="IWM23_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9885074.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IWM23_002476"
     CDS             378..1739
                     /locus_tag="IWM23_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAP9885075.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IWM23_002477"
     CDS             1761..2021
                     /locus_tag="IWM23_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885076.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IWM23_002478"
     CDS             2035..2787
                     /locus_tag="IWM23_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAP9885077.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IWM23_002479"
     CDS             complement(2881..3486)
                     /locus_tag="IWM23_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9885078.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IWM23_002480"
     CDS             complement(3539..4513)
                     /locus_tag="IWM23_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAP9885079.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IWM23_002481"
     CDS             complement(4891..5670)
                     /locus_tag="IWM23_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9885080.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM23_002482"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IWM23_002482"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAP9885081.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IWM23_002483"
     CDS             7161..8558
                     /locus_tag="IWM23_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9885082.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IWM23_002484"
     CDS             8639..9079
                     /locus_tag="IWM23_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAP9885083.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IWM23_002485"
     CDS             9227..9553
                     /locus_tag="IWM23_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAP9885084.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IWM23_002486"
     CDS             9554..10303
                     /locus_tag="IWM23_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885085.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IWM23_002487"
     CDS             complement(10383..10838)
                     /locus_tag="IWM23_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9885086.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IWM23_002488"
     CDS             complement(11265..11540)
                     /locus_tag="IWM23_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885087.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IWM23_002489"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IWM23_002489"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAP9885088.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4055 a         2175 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACDXE010000088        12556 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.088, whole genome shotgun sequence.
ACCESSION   DACDXE010000088 DACDXE010000000
VERSION     DACDXE010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.088"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            368..1147
                     /locus_tag="IWM23_002490"
     CDS             368..1147
                     /locus_tag="IWM23_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885089.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1193..2137
                     /locus_tag="IWM23_002491"
     CDS             1193..2137
                     /locus_tag="IWM23_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885090.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2153..2932
                     /locus_tag="IWM23_002492"
     CDS             2153..2932
                     /locus_tag="IWM23_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAP9885091.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2944..3642
                     /locus_tag="IWM23_002493"
     CDS             2944..3642
                     /locus_tag="IWM23_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAP9885092.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3670..4434
                     /locus_tag="IWM23_002494"
     CDS             3670..4434
                     /locus_tag="IWM23_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAP9885093.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4478..5929
                     /locus_tag="IWM23_002495"
     CDS             4478..5929
                     /locus_tag="IWM23_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAP9885094.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5961..7097
                     /locus_tag="IWM23_002496"
     CDS             5961..7097
                     /locus_tag="IWM23_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAP9885095.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7097..8203
                     /locus_tag="IWM23_002497"
     CDS             7097..8203
                     /locus_tag="IWM23_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9885096.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8209..9231
                     /locus_tag="IWM23_002498"
     CDS             8209..9231
                     /locus_tag="IWM23_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAP9885097.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9253..10308
                     /locus_tag="IWM23_002499"
     CDS             9253..10308
                     /locus_tag="IWM23_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAP9885098.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10926..12161
                     /locus_tag="IWM23_002500"
     CDS             10926..12161
                     /locus_tag="IWM23_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885099.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12302..>12556
                     /locus_tag="IWM23_002501"
     CDS             12302..>12556
                     /locus_tag="IWM23_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885100.1"
                     /translation="MKNTIFSDEYVEIIISDHSKNTKEIAKKIEEKKVFDGCHYLESK
                     SFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFDEF"
BASE COUNT         4574 a         1670 c         2488 g         3824 t
ORIGIN      
        1 ccaacatgtt tagacatttt cctctaggaa aaaattaact agcaccacgg gtataactat
       61 tattaattca atgttaattt ttttgttatt ctattttttg tgtaactttt aaaaacacct
      121 atgaaaatta tggaaataaa aatgaaatcg attaattttt atcttattaa tgttttattt
      181 acttttaaat cgtaaattgc cctagtcaag aaaacagttt gtggtatgat tatataggga
      241 aaacttgttt acaaatattt ctgtgaatat attagtttaa ttatgttttt ttaaataaaa
      301 agctgtcatt tttatgtaag tgaattatat cttaatttat ctatcatcaa tacttaggag
      361 tgaatctatg gaaaagccat tcgtcaagtc attgactgct gtggcggtag gtgcaggtgc
      421 tattgcgatc tgtttgtttg gataccatat caataatcag cggcaacatc agcaacggat
      481 caattatgca gaatctgcca tcaccaatca aaaagacacc ttgacttctt tatcaaaaga
      541 agtagacaag ctgtatagta caaaagaaaa aatttttcta aaccctgaaa taacagaaga
      601 aacaatcaca agtttgtcaa ataagctatc ctctgtcaaa ctttcagcag atgattacga
      661 catcaaagaa tcagaattgc ctaaagaagc tgttgcaatc caagatgaga agaaagcaat
      721 cgtgacacag ttagatagtg cagaatcaaa actgaagatc cagacagctg tcaacgattt
      781 atttactaaa aatgtatcaa actggcagca agctgtagac gatgtcatca tcaaagaaaa
      841 ggtgacaagt gcagatgtcg ctcgtgtacg tgaaaatatg agctttttta aagatagtcc
      901 ttggaaaacg gttgttatgc aatatctagg attcgctgat attcagatcg cgcaagtcac
      961 gcaactagat cagttattcg atacgatgtt aaaggatggt caagtgactg ccaccgctac
     1021 ttacgatcaa tatttgacgg tgctcagtca aatagagcag atccggaatg aaaaaataag
     1081 cgcagcatac gcaacaaaag cacaaactgt tgctgagcaa atgggctata gcaatataag
     1141 ctattgacag ttaaaacgtt gattcaagca aggttagcaa acggaggaaa ctatggcaag
     1201 acgaagaaga aaatcaagag ggaaaaaaat atttctgatt attttagcag ttcttatcgt
     1261 attagtatta gcaattgtag gagtaggagc aaaattatat ctggatctct cacattctat
     1321 tgagaaaact tacgaatcag tggaacgaac ccaaggagaa cgaacgaaac cagttgatct
     1381 ggacaaacaa gaatctttct ctgttctatt gatggggatt gatactggag atttaggtcg
     1441 ggtcgatcaa ggaagatcag atacgatgat ggttgccaca gtcagtcctg aagacaaaca
     1501 gacaacgatc gtcagcattc ctcgtgatac gtatgtcgac atcgtaggtc gtgacacaaa
     1561 agacaagatc aaccacgctt acgcattcgg cggagctgcc atggcgatgg ataccgtaca
     1621 aaaatatgta gacatcccaa tcgatcacta tgtctctatc aatatgaaag ggctaaagga
     1681 actcgttgat gcagttggtg gtatcgaagt caataatgac ctcactttct cacaagatgg
     1741 ctatgacttt ccaatcggta aaattacatt gaacggggat caggcattat cttattctcg
     1801 tatgcgttat gaagacccaa atggtgatta cggtagacaa gggcgccaaa gaaagatcgt
     1861 agaaggcgta gcgaaaaaag tattgagtct aaacggtgtc agcaactatc aatccgtttt
     1921 gaatgctcta gagtcgaata tgaaaacaga catgaacttt gatgaaatga aaaaaattgc
     1981 tttagattat cgggaggcat ttggtacgat taagcaagat cagctgcaag gagaaggctt
     2041 catgcaagat aatatctcct atcaaaaagt cagtgatcaa gaacttgatc gtgtgaaaaa
     2101 agaattaaaa gatcaaatga atctagagaa taaataggaa agtggagaaa aaatggaaga
     2161 gacaatcagc ttgaaagaaa tttttgaaac attacgcaaa cacttggtaa taatcatcat
     2221 ctcaatgttt gctggcttgg ccatctcagg aatcgtaaca tttttcatct tgacaccaaa
     2281 atacagttca caagcacaat tgatcgtgac actgccacaa tcagacactg caaatgtcaa
     2341 tgatgtcaat acgaacctgc aaatgatcaa tacgtataaa gatatgatcg tcagtgattt
     2401 agtgctcaat gaagtcaaag atcgtttaga aacagaagac aatgtgaaaa tgactgctga
     2461 acaaatcaaa gatgccatct cagttaatca ggcagaaaat tcacaaatgt tctcgattca
     2521 ggcaataagt acgaatgcag tggctgctca acagatcgct aacacaactt cacaagtttt
     2581 ccaagagaac gcaaaagatg tgatgaatgt tgataaaatc tcgatcattt caggtgcagt
     2641 tgccaatacg acacctgttt cgccaaataa taaattgaat ttaatgactg gcttaatgtt
     2701 aggaatgatg gtaggtgtag gtctggcatt cttattggaa ttactcgatc ggacagtgaa
     2761 agatgaaaaa tatgtgacag atacattagg attcccaatc ttaggaacag ttccagaaat
     2821 gtcagcgaaa gaattgaatg ctggtatccg taaaaaagga ccagctcaga cacaaccaaa
     2881 agctacaaac gataaaacag cgccatcacg cagaagccgc tcaagagtct aggaggaaag
     2941 aaaatggcac gaacacagaa acaaaaagca gcccaaactc atgcagtcag tttggtcaca
     3001 ctcaccaatc cctcttcacc aatcgctgaa caatatcgta cgattcggac gaatatccag
     3061 tttgcctccg cagctggtca gcaaatcaaa acgattgttg tgacttcatc aggacctgga
     3121 gaagggaaat cgaccactgc agcaaacatt gccgttgttt ttgccaaatc tggtcaacgg
     3181 gtgctgctag tcgatgcaga cttacgtaag cccgtcatct ataaaacgtt caagttgaac
     3241 aacgcaagcg gattaagtac tgttcttagt tcatcaggaa gtgtagcaga tgagatccaa
     3301 cgaacaccag tcgacaattt gtcgattctg ccaagtggac ctaaaccacc aaatccatca
     3361 gaactactaa gttcgccaag aatggatcag attttagcag aagcaagaca actatttgat
     3421 gtagtgatct tcgacatgcc gccagtcgtt gccgtaacag atgcacagat catgtcttca
     3481 aaaacggatg gcaccttatt ggtcgtacgg gaaaatacgt cacgaaaaga atcattgact
     3541 aaagcaaaag aactgttaga tatggtacaa gcacgagtct taggtgtcgt atacaacgga
     3601 gcagaacaca gcaaagatgc tggttattac tacggaaatt aaggaataag aagagaggag
     3661 acaagagaaa tgatcgatct acattgccat atcctaccag gaatcgatga cggggcagaa
     3721 aacttagaag caagtattgc aatggctgaa aaagcgattc agcaaggcat tacgcatatt
     3781 ttatgcacac cacaccacaa taatggaaaa tacagcaatg aaaaatcaca agtcatttct
     3841 ctagtcgctt cgttacaagc cgaattagag aaaagacagt tgcctttgac gttattggaa
     3901 ggacaagaag tacgtattac cggaacgctc atcgaagaca tccaccgaga tgaaatttta
     3961 tttacagatt tagatgatac atatcttctg atcgaatttc cgacactgga agtgccgtta
     4021 tatgcagaac ggcttttttt tgagttgtta gaactaggaa agataccagt gatcgtccat
     4081 ccagaacgca acgcgcattt tcgagaagac ccaaatcatt tgcttccgtt cctagatatg
     4141 ggatgtttgg cccaacttac tgcgccaagt tatgttggtg tatttggcaa agatatccag
     4201 aaaacagcca aaacaatggt caaacataat ctcgtgcaga tggctgcttc tgatgcacac
     4261 ggggtctcta agcgcacctt ttacttaaaa gagtgctatg agcagattgc caaggagttt
     4321 ggtaaggaaa aagtagtaca gatgaaacaa gtcgcaaagg atctgatcaa cggggatgag
     4381 atcaattatc caatctatga agaggtaaaa aagaagaagt ttggtttatt ttagttcttt
     4441 attcgtagat aaaaaggaaa tgaggagaga gtgagagatg tatagaaaaa actcaagtgg
     4501 ttggtctaaa catattgatt ttattttatt agacttattt tgtgtccagc ttgcttttta
     4561 tatatcatat gtaatgcgac agggtgactg gaatccgtat gtgattccta tttatcgcca
     4621 catggcaatt tttatagaac ttgagaatct tacgattata tttttatttg aggcatataa
     4681 aaatgtttta aaaagaggat attataaaga ggctgtagct tctataaagc aatcttttat
     4741 gctgttactt gtgtgcagtt tataccttat tacaatgcag gatggaaatg attattccag
     4801 agtttcttta tttataatgg gggtggtgta tggattatta acatatataa ttcgtatact
     4861 ttggaaaggg ttgttgcatc accgcatgaa aaacggtgaa gatgtatcgt tagttattgt
     4921 aacatctcaa aagattgcaa aaaatgtagt tcgtaatctt aaagagaaga attatggcat
     4981 gtataaactt agtggtgtga ttgtagtaga cagagatatg tatggaacta ccatcggagg
     5041 tgtcccagta gttgcaaatg ctgataatgc agcggaatat cttttacaga attgggtaga
     5101 tgaagtattc atcaatgttg acgaaagtgt tccatatccc aaagaattaa tcgccagatg
     5161 ttcggaaatg ggacttgctg tacatatgaa tcttacaaaa gtgtctgata gtacacgtgg
     5221 aaaacaattt attggccgag ttggtgatta tactgttatt acatctagta ttaatgttat
     5281 gacgatgagg caagcattta taaagagaac gattgacatt cttgctggaa ttattggatg
     5341 tattgcaaca ggtgttattt ttatcttcgt agcaccagtt atttacatca aatcaccagg
     5401 gccgattttc tttgcacaag aacgtattgg tcaaaatggt aagacattta agatgtataa
     5461 gttccgtagt atgtatatgg atgcagaaga acgcaaagca gagttgatga aagaaaataa
     5521 gatgagtaat aatctcatgt ttaagatgga ttttgaccca agaatcattg gcaataaaat
     5581 acttcctgat ggaacaaaga agacaggaat tggacaattt attcgatcta catcattgga
     5641 tgagtttcca caattttata atgtcctaat gggaaaaatg agcctggtgg ggtttagacc
     5701 ttgcttgaag tcagaatatg agaagtatga ttttcatcat cgtgcgagaa ttgcgatgaa
     5761 acctgggatt actggaatgt ggcaggtgag tggtagaagt gatatcacag acttcgaaga
     5821 ggttgtaaaa cttgatgcag aatatatcag gaattggagt atgggattag attttagaat
     5881 tttgtttagg acagtcaaaa ctgtgcttca tagaaacggt tccatgtaag ataaagatac
     5941 tatagaagga gtaaaacgtg atggcaagaa aaaaattaaa aatctgtatg ctaggtcata
     6001 aaagaattcc atcaagagaa ggtggaattg agatagtggt agaagaattg gcaacgcgaa
     6061 tggttgcact tgggcatgaa gtgacttgct ataatcgaag tggtcatcat gttagcggaa
     6121 aggaatttga ccagcataaa aataaagaat ataaaggtgt aaaattaaag actattttta
     6181 cattaaatat aaaaggaatt gcagcaatga gttcttcagt atttggtgga attagagcag
     6241 cattcggaaa atatgatgtt gttcattttc atgcagaagg accatgtgct atgttgtggt
     6301 tgccaaaact ttttgggaaa agatgtgttg caacaattca tggtctagat catcaaagag
     6361 aaaaatggaa caagcttgct agtacataca tcatgttagg ggaaaagtgt gctgtaaaat
     6421 ttgcagatga aataattgta cttagtgaaa gcgtccaaaa ttattttgaa gatatatatg
     6481 gaagaaaaac aaggtttata ccaaatggcg ttaaaaaaat tgaaataaaa tcagcaggat
     6541 tgattacaga aaagtatgga ttaacaaaag attcttatat tttgtttttg gggcgattgg
     6601 ttcccgaaaa aggaattcgg tatctaattg aggcatttaa agatgttcag acagataaaa
     6661 aacttataat agccggagga agctctgata cagatgaatt tgcaaatgaa ctaaaggaat
     6721 tagcaaaagg tgatgagcga attatattta ctggatttgt acaaggacag gaattagaag
     6781 aattatatag taatgcgtat atctatacac ttccaagtga tttagaagga atgccattaa
     6841 gtttgttaga agcaatgagt tatgggaact gttgtatagt ttcaaatatc tcagagtgta
     6901 cggaagtagt agaagacaag gctatgattt ttaagaaatc ggatgtaagt gatttaaaga
     6961 taagattaga agaggcatgc aatcaatcgg aaatggttaa agttttgaag aatcaagcta
     7021 ctgagtttat ttgtagtaaa tataattggg ataaaatcgt acaagaaaca ttaaatttgt
     7081 ataggggaaa tatataatga ttcgaatatt acagtgtgtg aatgatatgc atcgtgcagg
     7141 gttggagacg atgctaatga actattatag gaatatagat cgaactaaaa ttcagtttga
     7201 ttttttgaca catcgtccat ataaaagtga ttatgatgag gaaataataa gtcttggagg
     7261 aaaggtctat tatgcaccga gactttatcc acaaaattat cctaaatatt ttaaatggat
     7321 ggaacaattt tttaaggatc atccagaata tgagattgtt cattctcata ttgatgcgat
     7381 gagttattta cctttaaaag ctgcaaaaaa agctgggatt ccgatacgga tagcacatag
     7441 ccataataca tcgatagata gagattttaa atatctttta aagcaatatt atcgatgtaa
     7501 gttaacgtct gttttgacac atgaattttc atgtggaaaa gtagcaggtg attttttgtt
     7561 tcgaaatgat aaattcactt tgattccaaa tgcagtggat gctaaaaaat tttattatga
     7621 tgaaagtatt agaaatgatg tacgaatgaa attaggaatt tcaagtcaga cgtttgtaat
     7681 gggacatatt ggaagaattt catattctaa aaatcatcgt tttttgattg aaatttttaa
     7741 agaatttcac gctattaaga ataattccgt attattaatt attggaactg gtgatatgga
     7801 agaagaaata aaaaattatg caaagaaaag tggcatagat gatgatatta aatttttagg
     7861 aaatagaaat gatatagaaa aattttatca agcatttgac gtattaatgt tgccatcatt
     7921 atttgaaggg gttccgttag tgggtattga agctcaattt gcagatttac cgtgtttctt
     7981 ttcggagaaa gttcctacag aagttgcttt cagtgataag accaatttca taagtttaaa
     8041 tcaatcggca aaagaatggg cgagagagat tgcaaatgta gatatctctc atcgagattc
     8101 agaacgttcg attttaataa aggcagatta taatattcaa acagcataca aaatcttaga
     8161 atcaaaatat tatgagttat ttgaattaat acaaagaggt taaagaatat gacacagaaa
     8221 cattcaagta taaaaagaat tatacatcgc atatccgaag aattgaatgc aacaagacga
     8281 attgcaaaga taacatcttg gaaggaagca tttgccacgt tacaagctaa aattgatatt
     8341 caagtcatga atcgtaatgg atataatgaa agtaaaaaac agaaggaaca tttattaaaa
     8401 aaacatgagg taatgctgaa gtattatgaa aagacttttt cagatttttt gaaaacatat
     8461 gattatagtc aaaatcaaat aattaatcaa tcaaaatcac catattctaa ctgcatttgg
     8521 gtttgttggt ggcagggatt ggatcaatcg ccggaaattg taaaagcttg tgtaaaatca
     8581 ataaaacgaa atgcagggaa acatactgtt ataattttaa cagaagataa ttatagacaa
     8641 tatgttgata taccaaaatg ggtggaagaa aagaaaaaca aaggaatcat tacgagaaca
     8701 aattattctg atttattaag attatctttg ctagcaaaac atggaggttt gtggttagac
     8761 gccacatttt attgtacaaa accaatacca gatgagtatt ttgatatcga tatttggtct
     8821 attaggaggc ctgattatgc tcacgcatcc gtagcatcag gatattttgc gggttattca
     8881 ttagcatgta atcaagagtc tagatggatg tttgctataa ttagagactt ttttttaaat
     8941 tattggaaaa ataatgatat gatggtggat tatttgatga tagattatat gattgtttta
     9001 gcacagagaa taaatcatga aatagcacag acatttgata aaatacctag taacaatcca
     9061 ttatgtgacg aattgattaa aaatttaaat aatatatatg atgaaaaagt atggaactta
     9121 ttaaagaaag atactttact gtttaagttg acatggaaac aatcttttaa aaagcaaaaa
     9181 gatgataaag ttacttttta tgggatgttg gtagaaaaca aattattata aatattagag
     9241 gtataaaaag ttatgaaaca caaggtaagt gtgatagtac caatttacaa tgttgaaaaa
     9301 tatttaagaa aatgtgtgga tagtttattg agtcagacat taaaagagat agaaatcgta
     9361 ttagttgatg atggttcgcc agatgcttcg ggggaaatag cagatgagta tcaaaaaaaa
     9421 tattctaatg taaaaacaat acatagagaa aatggggggt taggaccggc aagaaataca
     9481 ggaattgaga atgcgacagg agaatatgtg gcttttcttg attcagatga ttgggtagaa
     9541 agtgatatgt atgaaaaatt atacttagtt gcgtccaaag aaaatgctga tatagtagtt
     9601 agtggacatt gtgattttgc gaatgacaaa gctatggtga aaaagcaaca tcctttgagt
     9661 gggaatgtat atgatacgcc taatagtatt aaaaatgtac gaaaaaattt atttggacat
     9721 agtccaaaag atcaagaagt agaggccttc ccgatgtcag tatgtatgtc tttgtataat
     9781 aaaaatctgc tagataaata tagactaaga tttgaaaaaa ttttatctga agatacaatt
     9841 tttaacttga atgcatatga tgtcgcaaat aaaattgctt ttacagaata tacagattat
     9901 tgttatagaa aagaagaaca agagtctatt acaaaaagtt tatctccaaa taagcagacg
     9961 gaatttaaag aatttttaat aactctaaaa gctatagcat cgaaagaaga tttagaatgt
    10021 caaatcagag caaaacgtat ggcaattgat tattgtaggc tttatgtggg cttagttgat
    10081 gatgcaaatt tgggaattaa agagaaaaga aagtatataa aaaaatttat tgaagatgag
    10141 ggattaattc aatgttggaa gggatatcct acaaatacgc taccatttca acaaaaattt
    10201 tttcataaaa tgcttgaatg taagtgttat acaatattgt tgttgttaag tagaattaga
    10261 aagataatta aaaatatgac cagaaaaaaa ttgtttataa tattataaac taaaagtata
    10321 tcaaataata gaaattaaca agttacaaaa aaatattata aagaataata ttttctaaaa
    10381 agtaaactat agtaatgtgg attatcacct tccaacagta tataaaatga gcgtaaaaac
    10441 ggctattttt gccttaacaa cgattttttt gtatacaaat acgatatgct cattaattat
    10501 tggaatttgt agtgctgttt aaattaatga aggtgtaatt atatgagtga tcattttgaa
    10561 aaagtttgtt gagaagatgt gcttctagtc atgtgatggg agaaaataat ttaactgtta
    10621 gagtgaatag ttaaattatt ctggttatat gaaaaaagta gtttctaaaa taatcttgca
    10681 gggattaact taaagaaatt gagagaatag tttctttgaa atttctatat acaatgagaa
    10741 gctgaaattt acttttattt gatgactgga aatagaaatt aaatatattg tattgttaca
    10801 ttaaagaaaa cttgtatgga aaaatcagta caataccatt atttaatgtt cttcacttaa
    10861 agcgcaaaaa atatcagttt attaaactcg tatatagaac aaatatatgt agacggggcg
    10921 aaacgatgag tgtaaaaaaa atgctattta tggtaagtac aataattgta ttaacatccc
    10981 gattttgggg aattaatact agtttatcta tgcgatattt tattatggca atatgggatt
    11041 tatattttat gttaagctat ttttcttatc gtgagaaaac atataaaaaa tacgttatca
    11101 agaataactt ttggttgact ataatgccgt ttgtgatatt ttctatctat actctaataa
    11161 tatggggaat tggttcaaat gcagaatttg aaaattttat aaagttatgt tccacgttgc
    11221 tttatttaat tcttgcatat ggttacgcat gtacagcatt ttacttattt aaggcagaag
    11281 gaattgattt tattttttgg tcaggtgtta tttcatatat gagtggttca gttttttatt
    11341 taattgtatc ttatggttta aatggtttaa tttcttatat aaaaagtctt ataacaggtg
    11401 aaacaaatac tgcaaatttt gctatggagg tacatgattt aacttttgcg atgggggttt
    11461 ttttccttta ttacgtattt tttgaaaata aaaatacaaa aaaacataag ataaaaatta
    11521 tcgtatcttt aattttaatt ttcatgggat tgaagagaat tgaactgtta gctttaagtg
    11581 gtgcaattct agtgtattat gttttattaa agtggggaaa aaagattcaa atccgtgcat
    11641 tagtgtgtac tatttgtgca actgttattt cgttagtgtt tatttacatt atagatacag
    11701 gaattttggc attgcttatg tcaaatttgg gcattacaga tgacggtgcg aggctttcat
    11761 tttatctata ttcatctaaa ttttatgaac ttggtttagg atatgtagga acaggatgga
    11821 catggttttc aaaatacttt tttaatcttt atttatcgca ctttaggtta aatggtggaa
    11881 gaattgcagc aagcctacac agtgatatat taactattta tatagaagtt ggatgtatag
    11941 tttttattat atggatgttt tggttatttt ttgtgaaacc gataagacta actaaaaaat
    12001 ttggattgaa aacaggggag tgtgtattat tattaactat atatatgttt atattatatt
    12061 tgacggataa tacgctcact tatccagaca ctcaaatgat ttttctctta gtacctatga
    12121 tagtttcgtg ggttgataat caacaacgaa tatcggaata gatagctaaa gtatatttta
    12181 attatgaatt tataattagt tgtaaaagtt aatatttaac ggacaggaag tagggtgaaa
    12241 aaatttgaaa aaaattttag tgttagcaaa tacatattat caaataatag tagcaataca
    12301 aatgaaaaat acaatttttt ccgatgagta tgttgaaatt attatttctg atcattctaa
    12361 aaacactaaa gaaattgcga aaaaaataga agaaaaaaaa gtgtttgatg gctgtcatta
    12421 tttagaaagc aaatcttttg atgataatat tgggtatttt caaaagatta aggactgtct
    12481 tcagatttct tgtggtaaat caaatcgata tgcctattat ttgaaaaact gtaaaagtga
    12541 atattttgat gaattt
//
LOCUS       DACDXE010000089        11328 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.089, whole genome shotgun sequence.
ACCESSION   DACDXE010000089 DACDXE010000000
VERSION     DACDXE010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.089"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..675
                     /locus_tag="IWM23_002502"
                     /pseudo
     CDS             316..675
                     /locus_tag="IWM23_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(970..2103)
                     /locus_tag="IWM23_002503"
     CDS             complement(970..2103)
                     /locus_tag="IWM23_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885101.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(2094..2762)
                     /locus_tag="IWM23_002504"
     CDS             complement(2094..2762)
                     /locus_tag="IWM23_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9885102.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWM23_002505"
     CDS             complement(3218..4204)
                     /gene="lacD"
                     /locus_tag="IWM23_002505"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAP9885103.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWM23_002506"
     CDS             complement(4217..5143)
                     /gene="lacC"
                     /locus_tag="IWM23_002506"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAP9885104.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWM23_002507"
     CDS             complement(5156..5671)
                     /gene="lacB"
                     /locus_tag="IWM23_002507"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAP9885105.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWM23_002508"
     CDS             complement(5690..6118)
                     /gene="lacA"
                     /locus_tag="IWM23_002508"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAP9885106.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            complement(6138..6911)
                     /locus_tag="IWM23_002509"
     CDS             complement(6138..6911)
                     /locus_tag="IWM23_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9885107.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            7076..7555
                     /locus_tag="IWM23_002510"
     CDS             7076..7555
                     /locus_tag="IWM23_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAP9885108.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            7612..7914
                     /locus_tag="IWM23_002511"
     CDS             7612..7914
                     /locus_tag="IWM23_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003088862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HAP9885109.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            7936..9393
                     /locus_tag="IWM23_002512"
     CDS             7936..9393
                     /locus_tag="IWM23_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HAP9885110.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNMTYAAEAK"
     gene            complement(9986..10738)
                     /locus_tag="IWM23_002513"
     CDS             complement(9986..10738)
                     /locus_tag="IWM23_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAP9885111.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(10953..11189)
                     /locus_tag="IWM23_002514"
                     /pseudo
     CDS             complement(10953..11189)
                     /locus_tag="IWM23_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
BASE COUNT         3257 a         2445 c         1933 g         3693 t
ORIGIN      
        1 tataaataac tcataaaaaa tattttgact ttcaaaatca cttttgctac cagaagttga
       61 ggcgaagtgt tttaagcata gttgtaccct agtttgaccg ttaaaaataa ataacgatcg
      121 ctagagtgca actatgtttg tttgccctac cgtcagcgtc ggcttggcct ctcacatcca
      181 ttaccgtcgg gattatctcc gttacatacc cttacaaaat acgccctgta agcattgcat
      241 gaaaccaccg tgcatttgca ccgtatttcc gccctagccg ttcaaatcgg tcgctaccac
      301 cattcagaaa cacgtgcggt aatggaaggt ttggaggaag ctatccagac cactaacgat
      361 tgtccctatc gtcgtacgtt tcactcagat caaggttggt cgtatcaaat gaaagcttat
      421 agaaacaaat tgaaggagta caaaatcttt caaagcatgt ctcggaaagg gaactgctta
      481 gataattcac ttatggagaa tttttttggc ttattaaaac aagagatttt tcacgggaaa
      541 gtttataact gtttcgagga attaaaatca gccatcgata gctatatcta ttactacaat
      601 aatgaacgga taaaacagaa attaaactgg caaagtcccg tccaatttcg taaaacaacc
      661 gttacggttg tgtaaaaata tcattttaga gcatgaaaaa aagagtggat ttctccactc
      721 caaaaaagtc taacttttgg gggtcactac atttttgatc ctttttattt gattaattat
      781 atccagctgg ataataattt tgggggcaac atttctatct tactattatc ttcctaaagt
      841 agtagacttt cttattggga aaaattacaa caaaaaggag tggcttagtt gacaactcct
      901 ttttgtttac aaatcaattg gatatctata aattgtattt tctaacgaat tttatagaca
      961 tctgatttgt caaattaagc tagacaatat gtctttatct atgcagctca aaaatacttc
     1021 caatggtgtt cgataattta atgattttct tggaatatgg tttcttttcg aggcaacgga
     1081 ttgaataaag gtttcatccg tgtccctaaa gtccattgtt ttaggtaagc catcctgctt
     1141 gagcccatgc actcggtact gatgagaaaa aattgatgcc tgtttctcct gaggaatgcc
     1201 aattccctga tcttttactt caataatcac gctacccttt tggtttgaaa tggtgacaat
     1261 taccagtttt ccttcaccat atttgattgc attgtcaact aaattcgaaa ccgcccgata
     1321 aatccaacga tctcgtccat gaatcagggt tggttgatct tcagtaaacg caaacaaaag
     1381 attcggataa ttcattttgt aggcatcaca aacttccgca cagatcagtg aaacgtctac
     1441 gacttccgtg ctaagatcct ctgagttatt ggataatgtc aaaatatcgt caatactgtc
     1501 ggtcatccga tctaaaagca ttaaatttcc ttgatcggca tgttgccgtt ctatttccag
     1561 attcgtacgc aaaatagcga tggcattttt ctgctcgtgg gtcagataac tatttaatcg
     1621 tcgataatcc tccaaatgac catcgaactt atcctttagc ttattaaaag ccgcatttaa
     1681 tgcctcgtcc ttcacagtat ttttaggaac ttgatcaata aacgttaaat cacgcgtaat
     1741 cgctaagatt tgattactgt aaattttgcg taaaaccaac caaagaataa acgttaataa
     1801 catgacggca gaacaaataa ccagcaccat cgctggtaaa tatttaaata gttcgtcctg
     1861 gtaagcttta tctatcatcg aaatagtcat aaattgattg ttggaagatt tcgaagcttc
     1921 catctgcgtc tctgcttgaa ccgtcttacc ctcttccata aaataaacat cttcacctaa
     1981 ggtagatgcg atactgattc cctccaaatt atttctcaga aagaagaaac tcactaaaat
     2041 cacgcctgtc accaacagtg aaaaagccag catcgttaac gtcattttaa aatttaatag
     2101 catagctggt acccctttgc tcgaatggtt tttaagacct ctttttcagc tgcttcagcc
     2161 agtttctttt ttagacgagc cagatgcact cgcagaaccg acgaaaaagg atcaaaatct
     2221 tcatcgtaga catgttcaga aatttcctca gacgaaacaa ccgctggatg acgctgagcg
     2281 atgcacagta agatatcaaa ttccttgggt ttcaggacca cctctgtctt ttgatagctg
     2341 acccttcgac ctaatggatc aatcttcaaa gcaccaattg aaatctgcgg cagcgcacgt
     2401 ccatgataac gacgaataac cgcgcgtatt cgtgctttta attctagtaa ctcaaagggt
     2461 ttgactaaat aatcatctgc tccaaaatcc aagccttttg ctcgttcttc aatctcatca
     2521 cgtgcagtaa taatgataac tggactttcg acttcttcag cacggagact ttgtaaaaca
     2581 gataatccat cttttttagg cagatttaaa tctagtagta tcccatcgta tgaattgatg
     2641 aaggccttct cttctcctga ctcgccatca taagctacat ctacagaaat attctcctgc
     2701 tctaggccct tttttacgga taaaaccagc tcacgattgt cctcaaccac cagtacacgc
     2761 atgtatattc actcctcata cggatttagt aattacactt atgcttttta gcatagtaca
     2821 cgctcttctt caaagtccac gttctttttg tgaagatttg aagaaattca tacattctgt
     2881 taaaaaacac ataattaaga atgattttac tgtttgaaaa tacctagaat agaaaaagac
     2941 tggaaccttt cctccagtct cactactttc tatttgcttg gttttcttgt atatactttt
     3001 caaagttcgt tggtttatga ccaataagcc tcgttagaat actgaaaatt gccatcaagt
     3061 cattaagtac cacatgtaag aaaatctctc taaagaaaaa ccccttcgtt ttaactcact
     3121 tactatttta gaattcgttc ccctaataat gcagcatgat tttacgcttt atgtataaaa
     3181 aaagactgca agcaattttg cttcacagtc tgatttctta tgatagtgag cttgttaaca
     3241 cttccaccgg tgtagcagtt tctttcaata cggcatttaa ttcttcaata tttttacgcc
     3301 cttgcgtatt catccactcc atcgctgctt cttttccatc aacgatatag ctttctacac
     3361 catctgccca agtcgcacgg ccgcaaagta caccattgaa ggttgagccg gcatttttcg
     3421 cgaagcgcag tgtttcttgg aacaactctg cttttacacc ggcgcttagg aaaataaatg
     3481 gcaggttagt ggcttcagcc tgttccttga agaagttagc cgcttgtgcc tttgtgtaca
     3541 cgacttcgtc tgtcccatag ccttccacat aattcatatt gacgggtact tccaccttta
     3601 acacatctac gccataacgt ggatcagaaa attccttcat catatcgttg actttatgag
     3661 gtttcttaat ggcgtattct ttcgaagtcg catcagagat gttcgcgtca taagaaacaa
     3721 gctccaagaa gaagggtaag ttttcagcct gacattctga gccgattcgt tcaataaagg
     3781 catgtttttg attattgatt tcttctgctt catcgacatc atagtaaagt aggaacttac
     3841 aagcatcggc gcctgcttct tttaaacgtt taactgacca gatcgataat aaatctggta
     3901 aacggccggg agttgacgca tcgtaaccag ttttttcata agctaacagc aagccggcat
     3961 tttttgctct aacgtcagct gctggcaagc catattcggg atctaataaa atcgatgagg
     4021 catatttcgt taattgtgaa gaaacaattt ctttaaattc aacgatttct gattcttctg
     4081 cttcgctgcc cttgaatttt gtgatcatct tcttcaaagc cccgcgttga tcaattgcta
     4141 aggccccaat cactccatct tcattgcata gttgcttgat atattgacgt ttttcttcgt
     4201 tcatttccgt atcctcctac actttctcca ctaagatttg atcatatagt ttttgataat
     4261 tggctaattg gacatagcca gtcgtcgctt cctgagcatt cagcattcct aaaacattac
     4321 cctgttttaa cacggcttca tccgaaagac cttgttgaat cgcagcggtc aatccggcaa
     4381 cggtcgcatc accagagcca actggattga cgacatcaat ctttggaatt gtcacgcaat
     4441 aaaaagtctc gtgatgtttg gcaaaggctc cgtcgccccc cattgaaacc actatccatt
     4501 caatttcatc aaacatcgaa tctgacaagg cggcttttaa atctgataat tctgttgaga
     4561 tttctcgtcc tagcagacca gccagctctt cggtattggg ttttattagc ttaggcttta
     4621 cactgcctcg caacacttcc cttaatgctg tacctgaaca atctaaaacg acaggctttg
     4681 gctgacaaac ttgcagcatt tgtcgataaa aatcagcagg caatccttct ggcaagcttc
     4741 ctgaaaaaga aatgatttcg gctttaacag ctaatttttc gaaatgttgc aagaacgccc
     4801 ttgcttcttg ttcagtaata acaggtccgt tttctaggat ttccgtctgt tttccgtcat
     4861 gtaaaacagc gatgcaatta cgtgtctctc cgctgatagg taaaaagtca tgagcaatct
     4921 cattttgagt caggtccttt ttaatctctt cacctaaaaa accgccgatt aaaccactag
     4981 caatcacctc cgtatctaat tgcttcaaga tacgtgtcac atttaggccc ttaccgccag
     5041 ctgttttgcg aacatttctt actcgattga ctgtgtcgag cttgaaggtt tctaatggat
     5101 aagaaatatc aatcgatgga ttcatcgtta ttgttaaaat catttgtcca cccgcttaat
     5161 cgtggtattc gccacgatcc catttctcga taaattcatc gaagaaatgc ggatcagact
     5221 gcttgtcact ggctttttcg acgtgatcga ttttggcgat caattttttg ttttcttcag
     5281 tttcttgata atcggcattg atgaaggcct ctaatatgtc acagatcaaa aattctcccg
     5341 taatccgagc gccaaagccg atcacattgg catttagttg ttccttcgca tagatagcag
     5401 tcgtcatatc gcgaaccagc gcactgcgga taccaggtac tttattgaca gcgttgttaa
     5461 tcccaacacc tgttccacac aaacaaactc ctagatctgc ttctccgcta gtcacggcct
     5521 ctccgacttt ttttccgtag atcggataat gggttctggt aaagtcatag gtccccatat
     5581 ccaacacttc atgtcctttt gattttaaga aatctgatac tgccattttt gtatctgtga
     5641 cgatgtggtc acagccaatt gcgattttca tttcattcat ccttcctaat tacgccattt
     5701 tatttaacat gtcgacacgt acttgatgac gaccaccatc ataatttgct tgtagaaatt
     5761 ccttaacaat attcaacgcc aactcatcgc caacaatttt tgatccaagt gtgatcatcc
     5821 gagaattatt atgtttacgg gtcatatacg cactgcgctc atccgaaact tctgctgcta
     5881 ccattccttt aacctttgtt gccgtgataa agctgccagc gccgtagcca tcaaccgcga
     5941 ttcctaaact accttcgttt tcattcagat catttgcgat cgccaaagtt gattccacaa
     6001 aatcttctga agccgtttca ctcttatcta ctactgtaat accttctttg atcaattttt
     6061 tcttgattaa ttcctttaat tccatgccgt cactgtctga accaattact acacgcattt
     6121 ttgtttcctc ctatttttta ctgaatgatt ttagtgaatt gctgataata actcttcagc
     6181 tcggctgaaa gagtagaatc cgtaaataat ttgtcgatct tgcgcaaatc atagaaatta
     6241 taaaaatcgc tatggtcgaa tttgtttgca tctgaaacca aacatttttc tgccgcatta
     6301 tctaaaacaa ttttttgcaa ggtaccttct tcaatactga aattcgaaac ctccgaaccc
     6361 ttcaatccat tcactccgac aaaagccttc ttaataccga ttttttgaac agcttcattg
     6421 gcaattgaac cgacaaaggc tccagtcttt tcccgatata gcccaccgat caaatacagt
     6481 tcaaatcttt ttatttcact taatagattg aaaaccggca gactattggt aacgattcgg
     6541 agagatgacg gcttgatgta tcgtgtcatt aattcgatcg ttgtacccga tccaagaaaa
     6601 atcgtgtcgt cttcttgatt gatatgagct gccgcatttt tagcgatgta ttccttctct
     6661 tttgtatgca gcttcttttt ttcagtatga gaaagctcat ttgcttctat agaagtcgac
     6721 atttccagat aatcgatttt ttgtgcaccg ccatgaacgc gtaaaagctt tttcttctgc
     6781 gcgagttcct ctagatctcg gcggatcgtc atatctgaaa cattcagtac ttcctgaata
     6841 aatttgactg ttatcgtccc atagcgatca cacatcgcta agatcgaacg ctgtcgttct
     6901 gttttcagca tattctgtac ctccacgacc ttataataaa acatttacga acatttttca
     6961 acacatattg ttcgtttatt ttcgtttatg cgttgacaat gattttgtaa gcggttatac
     7021 tcaacttgta aacatataaa taaacataaa cgaactaata aaggagctgt tttgtgtgaa
     7081 tgaattcttt ttcaaagaca ctgtctatgt ttctgacaag cagtcgcagg aagaagtgtt
     7141 cgaggaagtc tatcaagatc tgttaaagaa agaattggta accgaagatt ttttggagaa
     7201 ccttttagaa cgggagcgta attatccgac gggattagat ctctcacctg tctctaaaat
     7261 attgccagat atcgctatcc ctcataccga aagtgagttt gtgcgaacca cccgtatcat
     7321 tccaatcaaa gtaaaaaatg aaatcccttt ctacaacatg atctcgccaa gcgatcagat
     7381 ttccgcttca tttcttttca tgatcttgaa cgaaaatggt gaagcacagg ctggcatgtt
     7441 ggctaatatc atggatttca ttaattctgt tgacgaagaa aaactgtttt cgtttttcaa
     7501 attagaggac acagaagcga tttatcattt tttagaagaa catttttata tatagaagtc
     7561 tctgcctaac cgcaggataa aaaataaact tattttaagg ggagaattat tatgatcaaa
     7621 attttagcag catgtggagc aggcgttaat tcaagccatc aaatcaaaag cgctttggaa
     7681 actgaattga aaaatcgtgg ctatcaagtc acctgtgacg ccgttatgat caaagatatc
     7741 aatgaagaaa tgctgtctca ttatgacatt ttcgcacaaa ttgccaacac cgacttgggt
     7801 tttgacatta acatccctgt cgttgatgca ggtgctattc tttatcgtat tcctgctatg
     7861 gctgagcctg tttaccaaag tatggaaagc gcaatccaat cattaaacaa ataggactaa
     7921 agaaaggaaa agcatatgaa tacaattatt gatattgcca ataaagtatt taccccccta
     7981 attaatttag gtgcggcacc gttaatgacc atcgtattaa ctgttattgc tttatttttt
     8041 aaagtaaaac cgtcacgcgc tcttgaaggc gggctcaaat tagggatcgc cctgactggg
     8101 atcggagcta ttattggaat tttaactact gctttttcag acgctatgac agcttttgtt
     8161 gaacgcacag gtatttcatt aaacattaca gatgtcggtt gggccccact agcaaccatc
     8221 acatgggggt cgccatacac gctttatttt ttactaatta tggtgatcgt caatattgtc
     8281 atgttagcaa tgaaaaaaac caacacatta gatgtggata ttttcgatat ttggcattta
     8341 gcaattgttg gtttgttcgc gatcttctgc ggtgctaatc tgtttattgc ttctgcatta
     8401 gttattttca ttggtgtatt aaaaatttgg aactccgact taatgaaacc aaccttcaat
     8461 gacttgttaa atgcgccaga cagcaacccg atgaccacga ctcatatgaa ctacatgatg
     8521 aatccgatta ttatggtttt cgataaaatt ttcgacaaaa tcttcccttg gttggataaa
     8581 tatgatttcg atgctgccaa attgaatagt aaaattggct tctggggatc aaaattcgcg
     8641 attggaattt atttggggat ttttgttgga ttactagcag gacaaacacc aacgcaaatt
     8701 ttctcgctag cctttaccgc tgccgtctgt ttggaattgt tctctttgat aggcgcttgg
     8761 ttcatcgcag ccgtagaacc gttatcacaa gggattaccg attttgccaa taaacgacta
     8821 aaaggacgta ccatcaacat aggtttagac tggcctttca tagcaggccg cgctgaaatc
     8881 tgggctgccg ctaacgtatt agcaccaatc atgttgctag aagccatcat cttaccagga
     8941 aataaattat tgccactagg cggaatcatc gcaatgggtg ttaccccagc actattggtc
     9001 gtaactcgtg gaaaattaat ccgtatgatc gtaatcggcg cgatcgaatt accattattc
     9061 ttatggtccg gcaccttgat cgccccgttc gttactcaaa cagctaaagc agtcggagcc
     9121 tttccagccg gcttgaacgg gtccgccatg atctctcata caacgatgga aggcccgatt
     9181 gaaaaattcc taggttattt agtcggtaat gcttctcaag gacaaatcga atttgtgata
     9241 tacgctgcct tagcattagg tgcatacctt ttaatcttca tttggtacgc tcgtcaaatg
     9301 aaaaaacgca acgctgcata cgcagctgaa aaagaagaga atgcatctcc ggcaacggcg
     9361 cataatatga cttatgcagc cgaagctaaa taagctaaaa acaccccgaa tgtctttaga
     9421 cttcggggta ttattttacc tataaaaaat aaatagatgt aaaatccatt tcaaaaagca
     9481 tagcccatct cttgccacac ccaataaaga aattatcaac gtttatgctt tttttaactc
     9541 ctttaagctt gcctttgttg gtattcctca acgattcttt ttgtgttctt gtttgttttg
     9601 tgctttttga ctttttgttg tttaattgcg aattccttga tttctttttt cctgaatcgc
     9661 ttcgcatccc gcggttatgc gtgcgatttc atcattgctt tttctgatct aactcgtttc
     9721 atgtcaaaga gcttcagctg acttagtgtc catggttgtt ttgatatccc ttttcctcct
     9781 taataaagcg gtgattgata ctgctatttt ttccttcaca taaacataac atcccaaaaa
     9841 aatcgactat cttttttcaa atggtgtatt caagtattcg gaggaattag taatgatgca
     9901 ccctaaaaat aacataattc gcttaaattc aaggctacca aaaaactacc aagttctaat
     9961 tatctagagt ttggcagttt ctcatttaca caacatattt ttcttctttt tcgatttttt
    10021 catagtgctt ttgttcatca tcatccgtta taactactgt tacatcattc agattataat
    10081 atactgaaaa atctcttttc ccaattttcg aatgatcaat caataaaaag cgttcaacag
    10141 aattattcag agcgatctgt tgagtttggc cctcttcaaa agtcgaagtc ataattttct
    10201 gttcatttaa agcattacag ctgaaaaatg ctttatgaaa attcatatcc tgtagcgatt
    10261 tattcgtgat ttcaccaaca aagcattgag tcgtttcccg catctcgcca cctaatagat
    10321 agatttttcg attccctttt cgcttattta attcttcaaa aacaggcaaa cagtttgtaa
    10381 caatccgcag tgaatcccct tgaatgattt ttgacatgag ctcgattgta gttcctggtc
    10441 caagaaatat cgtatcacca tcattgatca tttcattcgc cttttccaca atccgctgct
    10501 tttcttcttg cagcataatc tgtttttctc tatgagaaag ctccacatgc cgataaaaat
    10561 ccttcaattt cgctccgcca tggacgcgtt ctaataatcc ttgtttctcc atttcgtcca
    10621 aatcacgtcg gactgtcata tccgaaaccg aaaacttctc cataatatcg ctgactcgta
    10681 ctgtaccgtt aacctctaat aacgacatga tcgcttggaa tcgatccgtt tttaacattt
    10741 catcgcctct tttcttttta agactatcaa attcttaaga aaagtgctag aacaagtttt
    10801 cttaagaagc cttctgcagg aaggaaagca aaatttttca gaatttgcaa gctgctattc
    10861 taaaaaataa aaccgaacga gcatgactgt catagtgaca gccttgttca ttcgactttt
    10921 atctttaata aatcatttta tttaatctga agttattgtg caagttcaaa aacaaagtag
    10981 ttaatgtctc ctcgatattt cgaaatcgaa tactcaacgg gcagacgttc tagcgaaaaa
    11041 ccctgtgtat ggaaatagaa cattggatct ccttcttcca catctaacaa atcgctgatc
    11101 gtatcatccg ctttgattac ttctagtttt ctggtgaccg ttttaattgg ataacccatt
    11161 tcttcaaaaa cttgatacat tttttctttt ctgatttgtc aaattaagct agacaatatg
    11221 tctttatcta tgcagctcaa aaatacttcc aatggtgttc gataatttaa tgattttctt
    11281 ggaatatggt ttcttttcga ggcaacggat tgaataaagg tttcatcc
//
LOCUS       DACDXE010000090        10931 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.090, whole genome shotgun sequence.
ACCESSION   DACDXE010000090 DACDXE010000000
VERSION     DACDXE010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.090"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..288)
                     /locus_tag="IWM23_002515"
     CDS             complement(<1..288)
                     /locus_tag="IWM23_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885112.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAEN"
     gene            430..1548
                     /locus_tag="IWM23_002516"
     CDS             430..1548
                     /locus_tag="IWM23_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAP9885113.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1676..3052
                     /locus_tag="IWM23_002517"
     CDS             1676..3052
                     /locus_tag="IWM23_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAP9885114.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3453..5033
                     /locus_tag="IWM23_002518"
     CDS             3453..5033
                     /locus_tag="IWM23_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAP9885115.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5231..5454
                     /locus_tag="IWM23_002519"
                     /pseudo
     CDS             5231..5454
                     /locus_tag="IWM23_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5659..5979)
                     /locus_tag="IWM23_002520"
     CDS             complement(5659..5979)
                     /locus_tag="IWM23_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAP9885116.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6127..8361)
                     /locus_tag="IWM23_002521"
     CDS             complement(6127..8361)
                     /locus_tag="IWM23_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAP9885117.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8840..9106
                     /locus_tag="IWM23_002522"
     CDS             8840..9106
                     /locus_tag="IWM23_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAP9885118.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9106..10833
                     /gene="ptsP"
                     /locus_tag="IWM23_002523"
     CDS             9106..10833
                     /gene="ptsP"
                     /locus_tag="IWM23_002523"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAP9885119.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1912 c         2216 g         3323 t
ORIGIN      
        1 gttttctgct tcttcaatca atatttttcc taacccttgg tgtctgtatt cagaaagaat
       61 cgccatccgc tgtaatttca tttttccgtt ttctaaaggc aaaagacgta cagttccttg
      121 cggttctttt ttatctgagt acaggacgaa gtggatacaa tgggcttcat ctttgtctat
      181 ttctcgagac aaaggcacac cctgttcgac tacaaaaact tgatgcctga tccgtacagc
      241 atctaagtaa atattgctca ttgtatcttt tgtttgtaca actttcatgg tctagccacc
      301 ttcctttcgt atagtatact ggaaaataaa acaaataaaa agtatggcaa gttttcaaaa
      361 ataaaaaaag aataaaagaa tttttaacga atataatata tactagtaag aaagaaagga
      421 ggaaatctat tgtttgaaaa acttagacag tctaaactaa tgttttggtc gcttgaatta
      481 ttgatcatag ctgcattgat catgatttca tcagaaataa actttatttt cgaaccgatc
      541 gggacatttt tttccacgtt atttgcacct gtactgattg caggctttct ttactattta
      601 ttgaatccta ttgtcaagct gttgatgaaa gcaaaaatca aaagaatatg ggctgtcgca
      661 attgtgctgc tccttttagt tgcagcaatt atttggattt tacttagtgt catcccaagt
      721 ctagtacagc aaatttcctc attggcatct aatatgcctg atttcatcaa acaggtagaa
      781 agctggttaa aagaggtagc cgaattgcct ttgttcaagg aagtagatat caataaatat
      841 tttgagcaat tagacatttc ttacgggaca atcattcagc aattccttag tggagtatcc
      901 aatagtttag gttcgatcgt tggaacgatc gcttcggcaa ccgtagtaat cattacagca
      961 ccattcatct tgttctatat gttgaaagac ggagaaaaac tagtgccaaa catccaacag
     1021 tttttcccag aaaaaagaag aaaccaaatt gtggagttat tggggcaact gaataaaaca
     1081 ttggctaatt acattagcgg acaagcaatc gaatgtttat tcgttggtac cttcacattt
     1141 ttaggatata tggtaattgg tgtggattat gctttcttat ttggggtcat tgcgggattg
     1201 acaaacctaa ttccatattt aggaccttat ctaggactag cacctgcatt tctagtaaca
     1261 gtcttcaatg atccggtaaa agcattactt tgttgtgtgg ttgtgctaat cgtccaacag
     1321 ttggatggga atatcattta tcctaatgta attgggaaat cactgaaaat ccatccatta
     1381 acgatcatta tcgttcttct agtagctgga aatattgccg gattgcttgg tatcttctta
     1441 ggtgttcctt tttacgcaat ctgcaagacg atcatttcct atataatcaa aatcgtcaaa
     1501 gaagacaagc aaaatgaaaa caagaagaaa attgctacga cctcgtaggt agttttagta
     1561 cacttgtttt tattgcaaag aaaattgctt tgtgatacca ttttaacgtg gctatagtgc
     1621 cacgtttttt tctatcgtaa actaaaaatt aattttataa ggagagaatg cgattatgaa
     1681 tgctgaccct gagagtcagt cgctgttagc gcagatttta ttattgatcg ttttgacatt
     1741 gatcaatgct tttttagcag cttccgaaat tgctgttgtt tcgatcaata agaatcgaat
     1801 cgagcaaaaa gctgatgaag gagatgtgaa atctcaaaag cttttaaaaa tattgcagaa
     1861 tccaaataat ttcctatcga caatccaagt aggaatcact ttggtcaaca ttttgtctgg
     1921 tgcttcacta gctaatactt tatcggaacg gttagctccg gtattaggtg gcggcacagc
     1981 ggcaaaaaat attgcaaata tcattgtgct ggctattttg acctatgtat cgattgtttt
     2041 tggagaattg taccctaaac gtattgcttt aaataaatca gaagaagtcg caaagttcac
     2101 ttcagggctg atacgcttga ttggtgtagt agctaagccc ttcgtctggc tgctttctgc
     2161 ttccacaagt ttattggcgc gtatcacgcc gatgactttt gatgatgaag attcaaaaat
     2221 gaccagagat gaaatgcgtt atatgcttga aaacgaaggc gtattgaata atgaagaatt
     2281 agaaatgctc caaggggttt tttctcttga tacgaaagtt gcccgagaag tcatggtccc
     2341 acgaacggat gcatttatga ttgatatcaa cgacactgtg gaagaaaatg tgaatgaagt
     2401 tttatccgaa aactattcaa gaatccctgt ttataacgaa gacaaagata aagtagtcgg
     2461 aatccttcat acaaagaatt tactaaaagc tgcccataaa ttcggctttg ataatttaga
     2521 tatcaagaaa atcatgcagg agcctttatt cgttcctgaa acggtcttta ttgatgatct
     2581 gttatatgaa atgaaaaaga cgcagaatca gatggctatt ttattagatg aatatggcgg
     2641 cgtcgttggg ttagcaacac tagaagacct tttagaagaa atcgtcggag aaatcgatga
     2701 tgaatcagat gaagttgaga atctttatga aaaaatcgat gaacacgaat atatcatcca
     2761 aggacgtatg ctgattgatg agttcaatga agcttttgac agtgatctgc atatgagtga
     2821 tgtagatacg atggctggtt atttgattac agcattaggc atgattccgg atgaaggcga
     2881 aaagttatcg tttgatgtcg ataatattac gctggtttct gaagaaatgg aaggttctcg
     2941 aatccttaaa atccgtgtca tttttcatga tccggaagaa acagaagcgg aacctgaaga
     3001 agaacgaaga tacttcagaa aagaattcga agatgacgag ccaagaagat aattggaaac
     3061 ttggttatga aagacgtgtt gcttgaataa acaagcaaca cgtcttttct tttagtcagc
     3121 tgacaaaaaa atatttgact cttatggttt ggtggctttt tcattcgctg aatccatgct
     3181 atactgattc agttgtagta gttgacaaag gttgtgaaaa agctatcctg cattaagtaa
     3241 taagagcagc caagcaaaaa cagcttttcg tatttttggc ttgggtgggc ttattgctga
     3301 taggctgtct ctagaacgcc gtttactcgg aaaaagagac tgctacacgg cttttgtcac
     3361 agccctatat gaaattgtct ttctttaaga gatcggacaa tctgtgccta gagaaaatat
     3421 aaataattga atggaagaaa tggagttagg ttatgaataa tccaaaatta aagcaacaag
     3481 tggacaatcg cagaactttt gcgatcattt cccatccgga tgctgggaaa acaacgatta
     3541 cagaacaatt actattgttt ggtggggcta tccgtcaagc cgggaccgta aaaggcaaaa
     3601 aaacaggtaa ttttgccaaa tcagactgga tggaaatcga gaaacaacgt gggatttctg
     3661 ttactagttc tgttatgcag tttgattatc aagggaaacg agtaaatatc ttggatacac
     3721 caggacatga agatttttct gaagatactt atcgtacttt gatggccgtg gatagtgcgg
     3781 tcatggtaat cgatagtgca aaagggatcg aagcacagac aaagaaatta ttccaagttg
     3841 taaaaaaacg tggcatcccg atatttactt ttatcaacaa actagatcgt gatggtcgtg
     3901 aaccattgga attgttagaa gaattagaag agctattaga tatcgaatct tatccgatga
     3961 attggccaat cggtatgggt aaagggctag aaggtcttta tgatatctac cataatcgtg
     4021 tagagtttta tcgtccggaa aattatcagg atgaacgatt agtagaattg gatgaagatg
     4081 gactgcttcc cgaaaatcat cctctaactg aaaattcctt atatgaacaa gtcttagaag
     4141 aagtagaact aatcaaagag gctggagatg catttaatca agaaaaaatt gctcgtggcg
     4201 atcagacacc agtatttttt ggttctgcat tgactaactt cggtgtacag acttttttag
     4261 agactttcgt tgaattggca ccagctcctt atggacacaa aacacaagat gatcagatgg
     4321 taagtcctta tgaggaagaa ttttcaggtt tcgtattcaa gatccaagca aatatgaatc
     4381 cagcccatcg tgaccgaatc gcttttgtcc gaatctgttc tggaacattc gaacgaggaa
     4441 tggatgtctg gttggaacgt acaaataaaa aactgaaatt aagcaatgtc acacaattta
     4501 tggcagattc gagagaaaac gtcgaaaaag cagtagcagg cgatatcatc ggtgtatatg
     4561 atacaggcaa ttatcagatt ggggatacat tgttcgaagg aaaactaaaa gttgcttatg
     4621 aagaactccc ttcatttact ccagagctgt ttatgaaagt gactgcaaaa aatgtcatga
     4681 aacaaaaatc cttccacaaa ggaatctatc aattagttca agaaggagcg attcagcttt
     4741 ataaaacata tctaacggaa gaatatatca ttggagctgt tggtcagcta caattcgaag
     4801 tattccaata tcgtatgtta aatgagtata acgccgaagt aatcatgtct ccaatgggac
     4861 acaaaatcgc tcgctggatc gatccagaag atttggatga gaagatgagt tcaagtcgca
     4921 atatcttggc tcgtgaccgt ttcgaccaac cgttgttctt attcgagaac caatttgcag
     4981 aacgctggtt tgcggataaa tatcccaatg taaaattaaa aagtttgatg taaagaagtc
     5041 ttgacacaag ctgccctgca tcaagtaata agaatagcca aacaaaaaca gcctctcata
     5101 ttttgtttag agccgtgacg gattttccgt gacggctctt tttactatat taaaatcata
     5161 gaaatgtcta atcaaaaaaa tataaaaatt attttgcgta catatgatta aagaaaggag
     5221 agtgtgacac atggaaacag aatggcaaag acgtgaaaga aaaaaacaga aaaagaagag
     5281 atgttacgat tatgtcgatt attagatgta aaagactggg acaaaaacga gcaaattgca
     5341 caaaaagtac gatcaatcat ccataaagag gaaaaagtta aaaataaaga cacgcgtaga
     5401 actgaaaatg aaaagagaaa agtacaaaag aaaagagaaa aatcaaaaag ctagaaagaa
     5461 aaagaggctg agacagaagc gtttaactcc aagaaataag ctggaattca caaaaatttg
     5521 aaaagcaatt ttcgtgaatt tcagcttatt tccgaaggag ttgcttctgc tttcgccgtt
     5581 tatacgtgtt tagagcgtga gacaaaagtg ttctttactt ttgtcccacg ctctttaatt
     5641 attatgttca aaatttgttt acacggctaa tgtagcgcct aattcatgtt ttggaatcga
     5701 gatttcaaca aggcctgcca attcgattac tttgatttca tcctcgtggt aaacggcgtc
     5761 tttgatttta tctaagaaat aatttttagc atcttcgatt tctttcggct ggattttacg
     5821 tttgtcattg atcagcagaa tttcattatg ttcaggggct tcagtgtaga caatagtggt
     5881 atttccggct gtgtagacct tgactagatg ggcatcagtg ttttcaagct ggtttaatac
     5941 aagacgggaa tggctgtttg ttacatttac caatttcatg gcgctcacct cttttatgta
     6001 tttttaattg cttgtttctc ttattataat ttctttttta taaaatgaac agtattttga
     6061 cttttctgaa aaaagaatga gacggcattt ctcaaagaaa taccgtctca cgctataaaa
     6121 ctggatttat tcagccgttt cttcggctga ttctttagcc aaacgttctg gtttagaagt
     6181 aacgatgatt tttccatctt tatccagttt tgctttcaac tcatggatag aagggtgatc
     6241 aagataaaat tcagcgatac catcttcgat ttgttcttgg atcgtgcgtc gaagaggacg
     6301 agcacccata gatggatcat agcctaagtc aactaatttt tctttgacat tcgtaggaac
     6361 ttcgatgtga agttgctgtg ctgccagcat ttggttgaca tcgtctagca ttagtgtgac
     6421 gattgtcatc aagttttctt ttgataatgc actgaattcg atgatcccgt caaaacggtt
     6481 cagaaattct ggtgtaaagt agttgtttaa ttggttcaat acagaacgag taacaccttc
     6541 gcgtgccgca ccaaaaccaa cgtttgcttc tactttacca gttccggcat tacttgtcat
     6601 aatgatgatc gtatctttaa agctgaccgt tcttccttgt gcatctgtca atcgaccatc
     6661 atctaagatt tgcaagaaca tatgcaaaac atctggatga gctttttcta cttcatctaa
     6721 caagacaaga ctgtaaggat ttctccgtac tttttctgtt aattgaccag cttcttcgta
     6781 tccgacataa cctggaggag aaccaatcaa tttagagaca ctatgttttt ccatatattc
     6841 agacatatcg aaacgaatca ttgaatcttg tgaaccgaat agttcgtagg caagttgttt
     6901 cgcaagctct gttttaccta caccagttgg tccaacaaat aagaaggagc cgattggacg
     6961 attctgtttg tttaatccaa cacggtttcg gcgaatcgct ttcgctactc gatctacagc
     7021 gttatcttgt ccgatgacat gagctttcaa gtcatctgct aagtttttca gttgtgtctg
     7081 ttctttttct tttaattcac ccactggaat acctgtccgt tgttcaacga ttttttccat
     7141 atctttttcg gtaatcacag gtgtttcctc ttcagtgagc tgacgttctt tcattttttg
     7201 aagtttattg atctgatcgc gataataagc tgctttttcg aaatcttctt ctcgagaagc
     7261 taaaactttt tgttcttctg cttcttgcag ttttttctcg attgttttag gatcaacgat
     7321 ttggatcgtc aagtttttct tagaacctgt ttcatctagc aaatcgatgg ctttatccgg
     7381 caagaagcga tcttggacgt aacgattgga taaagttgcg gctgcttcga tggcctcatc
     7441 agtatatttt acatgatgat agtcttcata acgtttttgc aaacctttca aaatactgat
     7501 cgtttcttcg acagttggtt catctacccg tacaggttgc atacgacgtt ctaaagcagc
     7561 gtctttttcg atgatgcggt actcatttaa ggtagtagca ccaaccattt gcaattctcc
     7621 tcgagctaaa gctggtttta aaatattacc agcatccata tttccatcac cagcagaacc
     7681 agcaccgacg atttcgtgaa cttcatctat gaataggatc acattttctg cttgacggat
     7741 ctcttcaatc aatttttgca tacgttcttc aaattgtcct cgtataccag taccttggac
     7801 taaagatacg acatctaaac gaatgacttc tttgtccatt aatttttgcg gtacatcacc
     7861 atctacgatt ttttgtgcta aaccttcaac aactgctgtt ttaccaacac ctggttctcc
     7921 aatcaagaca ggattgttct tggttcggcg atttaaaatt tcaatgactc gtttgatttc
     7981 gtcatcgcgt ccgatcacag gatcgatatc accttgtcgt gcttgttctg tgatattgat
     8041 tccgtattct cccaataaac cttctgtatt gccttgaggt ggctgaccac cattgaagtt
     8101 gttgtttccg ccaaattgtg ttggtggtgt ttgctcataa tttccttgct gttgcatttg
     8161 gcgagacatg gaacggaaaa gatcgtccaa actgccaaat ccaaatggat cattttgggc
     8221 catattagtc agacctccat tttgttgatt tttgattttt tgataacagc tctgacagta
     8281 atcgagttgt gtccgctgac cattgacagt ggcatacaag tgaatcgttg cctcattctt
     8341 gccgcagttt tgacaaagca tgaatcattc acgtcctttc acaaaaatgg gtacatccat
     8401 attttacatc tcgtttttca tgaggtaaaa gaataacccc tagtttttta agagatttat
     8461 gaggtagaaa aggtgaaatc tctattgacc attcctgacc tttgactctt attatactaa
     8521 aaataaaaag ggaagcaagt aattcgtttc atatgaaaac aaagagggtc aaaggaaaga
     8581 atgataaacg tcaggagaag agaaagcgac aagaaaaagg aaataaacac gtttgttata
     8641 aaatgtgaag aaaaggatga aaaagaatcc ggaataattt gaatactttt atctgaaatt
     8701 gaatgaaaac tgttgtatct aacgaaaaaa aatgttaatc tttacagatg aaagggtggc
     8761 atttgttagg attctctgaa ttactaaaaa agaaaaccct cacataaaaa caacactcac
     8821 ttaaaggaga ccgattaata tggaaaagaa agaatttcac gtagtagcag aaactgggat
     8881 ccacgctcgt ccagctacac tattagtaca aacagcaagc aaatttaact ctgatgtaaa
     8941 cttagagtat aaaggtaaat cagtaaactt gaaatcaatc atgggcgtta tgtctttagg
     9001 tgtaggtcaa ggttctgatg ttactattac tgcagaaggt gctgacgaag ctgatgcaat
     9061 ggcagcaatc gttgaaacaa tgaagaaaga aggcttatct gaataatggt tgaaatgcta
     9121 aaagggattg ccgctagtga cggtgtagcc gttgcaaaag cttacctgct agttcaaccg
     9181 gatttaacat tcagtaaggc aacagtagaa gatactgcgg ctgaagaagc tcgtttggat
     9241 gctgcattag ctaaatctac tgaagaattg cagcaaattc gtgaaaaagc agcgcaaagc
     9301 ttaggtgaag cagaagcgca agtatttgat gcccatttga tggttctttc agatccagaa
     9361 atggtaggtc aaatcaagca aaatattaaa gacaacagcg taaatgctga atcagcgcta
     9421 aaagaagtaa cggatatgta tatcggtatg ttcgaagcaa tggaagataa tgcttatatg
     9481 caagagcgtg ctgcagatat tcgtgacgtt gctaaacgta ttttagcgca cttattaggc
     9541 gttactttac caaatccatc aatgatcaac gaagaagtgg tcgttgttgc acatgacttg
     9601 actccaagtg atacagcaca attagatcgc aattttgtaa aagcgtttgt aacagacatt
     9661 ggtggacgta cttcacattc tgctatcatg gcacgttctt tagaaattcc agcaatcgtt
     9721 gggacaaaag aaattactgc taaagtcaaa gaaggcgata ttttggctgt caacgggatc
     9781 gaaggcgatg taatcatcga tcctacagat gaacaaaaag ctgaatttga aaaagcaggt
     9841 gcagattacg cagcacaaaa agcagaatgg gaaaaattaa aaaatgcaga aactgtcaca
     9901 gctgacggca agcattttga attagctgca aacatcggaa caccaaaaga tctagttggt
     9961 gtccataata atggtggaga agcagttggt ctgtaccgta ctgaattcct gtatatggat
    10021 tctccagact tcccaacaga agatgaccaa tatgaagctt acaaagctgt attagaaggt
    10081 atggaaggaa aacctgtagt tgttcgtaca atggacatcg gtggagataa agaactacca
    10141 tatctgcaat tacctcatga aatgaatcca ttcttaggat atcgtgcatt gcgtatcagt
    10201 ttatctgaac aaggcgacga aatgttccgt acacaaatgc gtgcattgct tcgtgcatct
    10261 gttcatggta atttacgtat catgttccct atggtagcta cactcaaaga attccgcgca
    10321 gcaaaagcga tctttgaaga agaaaaacaa aaattgataa gtgaaggcaa ggaagtatct
    10381 gatacgatcc aagtcggcat catgatcgag attccagcag cagcagtctt agcagacaag
    10441 tttgcaaaag aagtagattt cttctctgta ggtacgaacg atttgattca atacacaatg
    10501 gcagcagacc gtatgaacga acgcgtttct tacttgtacc aaccatataa cccatcaatt
    10561 ttacgtttga tcaaaaatgt aattgatgca gcacacgctg aaggaaaatg ggcaggtatg
    10621 tgtggagaaa tggctggaga tcaaacagct gttccattgc tagtaggtat gggtctagat
    10681 gagttctcaa tgagtgcgac atctattctt aaaacgcgta gtttaatgaa acgcttggac
    10741 acagcgaaaa tggctgaact tgctgatcgt gcattgaaag aatgcgatat gatggaagaa
    10801 gtcgttgaac ttgttcatga atatgtaaaa taaacaacgt atcaaaagct ggtgtggctt
    10861 gtaaaagagc tacgccagtt tttttgttct tttttaagaa aacgtttttg tcagtaagaa
    10921 tagacaaaaa t
//
LOCUS       DACDXE010000091        10532 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.091, whole genome shotgun sequence.
ACCESSION   DACDXE010000091 DACDXE010000000
VERSION     DACDXE010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.091"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..144)
                     /locus_tag="IWM23_002524"
     CDS             complement(25..144)
                     /locus_tag="IWM23_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAP9885120.1"
                     /translation="MEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKGK"
     gene            complement(159..1343)
                     /locus_tag="IWM23_002525"
     CDS             complement(159..1343)
                     /locus_tag="IWM23_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9885121.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="IWM23_002526"
     CDS             complement(1599..1853)
                     /locus_tag="IWM23_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885122.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="IWM23_002527"
     CDS             complement(1878..3020)
                     /locus_tag="IWM23_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HAP9885123.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="IWM23_002528"
     CDS             complement(3080..4432)
                     /locus_tag="IWM23_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAP9885124.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="IWM23_002529"
     CDS             complement(4503..4847)
                     /locus_tag="IWM23_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAP9885125.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="IWM23_002530"
     CDS             complement(4857..5231)
                     /locus_tag="IWM23_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9885126.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="IWM23_002531"
     CDS             complement(5244..5558)
                     /locus_tag="IWM23_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAP9885127.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWM23_002532"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="IWM23_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="IWM23_002533"
     CDS             complement(8976..9638)
                     /locus_tag="IWM23_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885128.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="IWM23_002534"
     CDS             10255..10485
                     /locus_tag="IWM23_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885129.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
BASE COUNT         2895 a         2344 c         1786 g         3507 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ct
//
LOCUS       DACDXE010000092        10471 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.092, whole genome shotgun sequence.
ACCESSION   DACDXE010000092 DACDXE010000000
VERSION     DACDXE010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10471
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.092"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(179..931)
                     /gene="xth"
                     /locus_tag="IWM23_002535"
     CDS             complement(179..931)
                     /gene="xth"
                     /locus_tag="IWM23_002535"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAP9885130.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1044..1964
                     /locus_tag="IWM23_002536"
     CDS             1044..1964
                     /locus_tag="IWM23_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAP9885131.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2037..2972
                     /gene="murB"
                     /locus_tag="IWM23_002537"
     CDS             2037..2972
                     /gene="murB"
                     /locus_tag="IWM23_002537"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAP9885132.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3270..4748
                     /locus_tag="IWM23_002538"
     CDS             3270..4748
                     /locus_tag="IWM23_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9885133.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5025..7087
                     /locus_tag="IWM23_002539"
                     /pseudo
     CDS             5025..7087
                     /locus_tag="IWM23_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7170..7739
                     /locus_tag="IWM23_002540"
     CDS             7170..7739
                     /locus_tag="IWM23_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885134.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7914..8720)
                     /locus_tag="IWM23_002541"
     CDS             complement(7914..8720)
                     /locus_tag="IWM23_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885135.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8858..10372
                     /locus_tag="IWM23_002542"
     CDS             8858..10372
                     /locus_tag="IWM23_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAP9885136.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3708 a         1744 c         2063 g         2956 t
ORIGIN      
        1 ctatttttgt ttggctgttc ttattacttg atgcaggaca gctttttcac aatctctcga
       61 taaacagtgt tcaaaagcca gtttctcggt attatgccaa atttatgcaa aatgtggaaa
      121 gccattttca caaaattttt cttaatactc gaaactaaac gactttttca caacctcttt
      181 atttcaaatc taattctact ggacaatgat cactgcccat aatgtccgta tggatctttg
      241 catcagctag tcctggctct aaatctttgc tcgtcaaaaa gtaatcgatc cgccaaccag
      301 cattgttttt ccttgaattg aatcggtagt tccaccaaga gtagacacct tcctgggttg
      361 gataaaaata acggaatgta tcgataaacc cgttatccaa cagtcgagaa agagccgctc
      421 tttcttctgg agtgaatcca gcattttttt gattcgtttt ccagtttttc aaatcaatct
      481 tttgatgagc gacatttaga tccccacaca cgatgactgg tttttgtttt tttagatttt
      541 ccaaataatt ataaaaggct tcttcccatt ctaatcggta atctaaacgt tttaattcgc
      601 tttgagaatt tggcgtgtaa caggttacaa gaaagaaatc ggaatattcc aaagtaatca
      661 gacggccttc tgtatcatgg atatcgatac ccattccgta agtggcattc aatgctggtt
      721 ttttagcaaa aatcgctgtg ccggaatagc cttttcgttc cgcgtaattc caatattgat
      781 aataacctgg aagatctaaa tcaatttgtc ctgcttggag tttcgtttcc tgcagacaga
      841 aaaaatcggc gtctaactca tgaaaaactt ctaaaaaatt tttatttacg atggcacgaa
      901 gcccgtttac gttccaagag ataaatttca ataaaagcac actccttcga attaatttcc
      961 ttacctttgc gatactattg tacctcattt tagtaaaaaa tggtgtaaga tgaaagtaac
     1021 taaatgatcc aggaggaaag tagatgaaga ttggtgtaat cggtgtaggg aatatcgcag
     1081 aaaaagcata tttaccaact tatgcgaaaa aacaaggaac acttgatttt tattttgcaa
     1141 caagaaataa agaaacaaaa gaaagaattc aaaaagagta cgggttccaa catctatatg
     1201 aaacgatcga tgagctttta gaagaaaaaa tcgaagcatg tatgatccac gcagcaacaa
     1261 aagtacatta tgaattagcc aaaaaatgtc tagaaaatgg tatccatgtt tatattgaca
     1321 aaccactgag tgtccatcta aatgaagttc gtgaattgca agaattagct gataagaacc
     1381 aaataatttt gatgatcggt ttcaaccggc gctttgctcc tatggtagaa gaattgaaac
     1441 gtatcccaga taagcggttg atccacctgc aaaaaaatcg aatagcggca aaagaaacga
     1501 cagagttcgt cctttatgat ttgtttttac atcttattga tacagctgtg tatttattag
     1561 atgaacccat tttgagaaca aaataccaga taagagaaac aaaggaattt ttggaatatg
     1621 ccattttaca attagaaaca caaaatcaaa cggctatttt aacgatggat ttagcaagtg
     1681 gcgctaatat agaaaaatat caagtaacaa gtaaaaatgg tacttatgaa gtaaacgatc
     1741 tgacccagct tgtcattcaa gatgctgatg gaaaaaaagt gaaagaattt ggcgactgga
     1801 caaacacgct agtcaaacgc gggtttgagc caatggtaga ggccttcttt tctcagatcc
     1861 gtttaggtaa atcggaaaca gaattgctta aacaaaaaga agttgtaaga agtcatgaat
     1921 tatgtgagga agtgcttaaa gatcactacc gtcatcaact ttgaacaaga ctggcaagat
     1981 tgtattaagc gtgatagaat aatgaggacg tttgagaaaa gaaaggaaaa tcctcagtga
     2041 ataaagaaga aatagttgaa agcttagcca cgattactat attgtttgat gaaccattga
     2101 tgaactatac gtttacgaaa acaggtggac cagctgatgt ccttgcattc ccgaaaaaac
     2161 aagaagaagt aaagcaaatc atcgattatt gccgaatcca tgatatacct tggatggtat
     2221 taggaaatgc aagcaatctg atcgttcaag atggcgggat tcgcggtgta gtcatcatgc
     2281 tgactgaaat gaaacagatt catgtcaaag gaacgatggt cattgcggaa gcaggtgcct
     2341 cactgattga tacgacttat gcagctttgg cagaatcatt gactggattt gaatttgctt
     2401 gcggcatccc gggaagtgtc ggtggagcgg tttatatgaa tgcaggtgcg tatggcggag
     2461 agatcaaaga tgtttttgct gaagtggatc ttttgcttga agatggaaca ttgaaaactt
     2521 tgacaaaaga cgaaatggta ttttcttatc gtcacagtaa agtccaagaa ttacgtgcta
     2581 tcgtattgga agctcgattc agtttgcaaa caggggatta tgaagcaatc aaagcaagaa
     2641 tggatgagtt gaccgaattg cgtcaatcaa aacagccatt agaataccct tcatgcggaa
     2701 gtgtctttaa gcgcccagta ggacactata caggtcagct gatccaacaa gcaggattgc
     2761 aaggcctgaa atggggcggt gcacaagtct ctgaaaaaca tgctggattc atcgtcaata
     2821 tcgatcatgc gacagcgaca gattatattg aattgattgc gcacatccaa gaagtaatca
     2881 aagaaaaatt tgatgtgtct ttagaaacag aagtgcggat cattggagaa aaacaagcaa
     2941 tatcagaaac aagcagaagt ttagccaatt aataagtaat ggaacttaaa ataagtaaaa
     3001 gagcaggacg gtggccgtcc tgctcttttt tgtggttgtt tacgcaaatg gcgcaaatct
     3061 tgtacatttt aacacaataa aacaaaaaat atagaatatt tcctcataaa tttttccatt
     3121 ttttgcaata ttctcaaaaa aaagataaaa aatgagaata actgatgaaa aaacaagcat
     3181 aatccatcat aaaatcagta taggtgaaaa gatgaacttt catctgcata tcaagtaatt
     3241 ggtatgaggc attttttgag gtgtaggata tgaagttgga ttaccttata gaagaggaag
     3301 atagagaaaa actgctgcta gctagatatt tagaatttga gaaaaagaat tatgtagtgc
     3361 tgcctaagtt atatgaaaca ttaggtttgt ctaaatttaa aacaagaaat tatatcatag
     3421 aattaaataa agaatttact gaattcaaaa caaagccaga gatccaaatt tcagaagaag
     3481 cagagatcca tgtcgaacgt ctgaatttga cggatatcaa aaatttaaga agaatctact
     3541 ttcaacgatc aaaaattgct cgactgttaa cggagatcgt ggattcaaaa gtaacgatcg
     3601 aaaaatttgc agagaatcat tttttaagca tatcaagagc ctatgtaaaa agaagagaat
     3661 tagtctcttt tcttgaacag catcaaataa agttaaagaa aaacatatta gttggggaag
     3721 aaataaacat acggaacgta ttatttgggg tatatttcgg ggtctttaat ggtttttcta
     3781 tgccatttag agaaagcgtc atgaatgaag caaagagtat cgtgcaatat tttgaatatc
     3841 tgttcaattt aaaactgtct gcaacaaaac gaatcaaatt caatttgcta accactatta
     3901 gtttgttgag ggcctatggg aatcaaccaa tcggaaaacg tttcttttca gatgaatact
     3961 ttaagagctc agaaggaatg aagatcatca aggatttatc acatatttct tttcttcata
     4021 tccctaaaga gcagatcatt gatgagattt cttatatcat gttgtttttg atcacggaag
     4081 aagaatttga attagatggc gcatttggac ttgcggaagc tcattttcat gatatccaca
     4141 tgattagtca aaaaatgtca gtggaattga ttgatcaatt accattttcg attgaaatct
     4201 ctgaagaaga aagactatat atacgaaaag aacttcgtat gggtatgatg catgtcaatc
     4261 gaaaaaattg gatattcgac tttgagatga ctagctttac gtctaaaaag cagctacagt
     4321 ttttcttcga gacgtatcca atattcagta cagcaatcca aaaggtcata caagagaata
     4381 aacgctattt cttgggaaaa gatgaacgtt tcatgattcg cttattttat gattatctct
     4441 ttttattggt cggtatttgt ccggtatcat attttgaagc accgattcat gtctgtattg
     4501 atttttcgca aggatctgct tataccgatt acattatttc tcaagtgaaa ggatttaaaa
     4561 attacaatat tgtgattgag agtcgaatca cttctcgtac gaatatttat ttatctgatt
     4621 gtttacttga aaaatttaat aaagaacaga taatttggaa aaatccaccg actccagatg
     4681 actgggaata ttttggaaat accatcatca gagaaaaaaa tagacattta actgaaaaaa
     4741 tcagctagta tttatggatg gcagctttgt ctcacatatt tattagctga tttatgtttt
     4801 tttcaaagaa aaggtaacta gatataacta gatataacta gatatattac ttaacaaaat
     4861 taaaaagtac ataagttatt attccgattt tcttgaaaca ataaaaaatc aaaaaaaata
     4921 ctctcctaat agtccatctt cttttgattt tatttatata atgaaatcat cttatgaaaa
     4981 ttactacttc atgtttagac aagagtaggg aggactaaga ataaatgtgt tcaaaaagac
     5041 gttcaaaaca actaaataaa cacgcttgtc gtttagataa ggaacggtcg tgttctgaaa
     5101 aacactacgt taagtttatg atgcgcacga tgctgatcag tttatctgta ggtgtctata
     5161 tgcatgtacc cgttcaggca acgacacaaa ccatcaaggt cgtacgtacc tctgtttctg
     5221 agaccactga ttcccctatc actattactc atactttcac tgatgcaaat ggaaaacagc
     5281 tacttcctgc attgacttcc tataataaag tcagttatcc tccaacgata aaccactact
     5341 ggttaattcc taacaaaaca ttatggacat cggaaaccgg aagcagtgtg ccgctttcta
     5401 tcttttccga tatgactgat cctacaaaag gtgatcagtt aaaacagatc actgatattt
     5461 tgaatcatgc tttgacagat ataaaggacg gtggaagcgc acaagtttct tatgtatatg
     5521 aaaaagatta ttcacagttt gaaagaaaga aaatcgatgt tctggcaggg acaaaagttt
     5581 cgatatctga tctggtagaa aggctgaaga acgcagatgg aacacaagga gatatgtcca
     5641 aagtaatttc aaacaatggg aatatcgtgg atacgactaa tacaggaaca tctactattt
     5701 ccttgtccta ttttgatcca attgctttaa aaacaatgac tgcatctgct gtgatccatg
     5761 ttcgtgctga tcaaacagct atcaaaggaa aagatcttca cttaagaaca ggagagagtt
     5821 acgcccctaa agatcttgtt gaatatgtaa caaaaagcga tggaacttca ggaaataaag
     5881 aagaaatcaa aatgaacggg acagtagata ctacaactcc gggagaatat cccgttgtat
     5941 taacttatat agactcgaaa acatcaaatg tggcagaaac aacagctgta gtttatgtag
     6001 aagcatctgc tgcttcatcg actgtcatca ctcattttat agatagtcaa acaaataaag
     6061 aacttcaagc agaaacacat atgagtagtg taaatcgtat tcctgatttg cgcctacctg
     6121 ctgaattttc tgatcgagtg ccaaatatat agatttatcg acttatgaac aaaatgggaa
     6181 agtcacttct atttctgatt ggatgcaagc aaacggatta gacagaaaaa gtcactggtc
     6241 ggagatcctt gaagtacttc actcagaact gatgaataat aggaagcaat caggagggga
     6301 aattaccttt acttatgttt atataccaga caatcgttca ttagaaggtc atgatcttat
     6361 tgttcatcca aacgaaaaaa taaacaaaga agagttgatc caatcagcaa caaattcctt
     6421 aggtaaaccc gtaccgttaa aggatgtatc ggttacaatc aatgaagtac cattggagga
     6481 tggttatttg accaaacaaa caggtgattt tacgattatg tatagttatc atgacccgta
     6541 ttcactccaa gaactccatg ctcagtcact attacatgtt ttaccattaa acaaacagat
     6601 agatcaaaaa gaacaaaaaa gacaagaaga aagcttgtca gaaaaaaaac aacaaatggc
     6661 tcatgcagtt tctccaacag ggaaagtaaa acaaacaaga agcctagagt tagcaagaat
     6721 atctagaaaa atctcagaat ctaataaaac gataaggcaa gaagctcatt tggccccaca
     6781 agtttctgga ttggaaaatg aaattttaga gcctaaaata aaacaacaat cagaaaaaga
     6841 atttgttgct aaacagtcgg aagatcagaa ggaatcagat gaccagaagg aaacgaagaa
     6901 aaaacgcttg tcaactattg attccaccga tgagcatctg cagccgcaag taccacttcc
     6961 tagtccggga acacccgcac ctggtggagg tattccttct tctgctttag gagatttcat
     7021 gcgaggtatg gcaggaacat gggtatacgc taatcgaaat gaatttgacg cagatgactg
     7081 gttctgattt atatctctat ctgttatagt caaataagta gctgagtcat atgttatttg
     7141 gcataatgga gacgaggagg gatagaaaaa tgttagataa aacgttacct tacgcagaaa
     7201 tctggatgac aagaccactt caacaaaatg ttccaatcac gcctttagcg gaaggttttg
     7261 aatttcgatt ttatcaaaaa gaagatgaat ctgcttgggc ggagattgag acttccgttg
     7321 gggaatttca aacgacagat gaagcacttg agtattttca acaaaacttt gctccttatc
     7381 ctgaagaact tgaaagaagg atgttttttg tagagacaaa aaaaggagaa aaagtagcaa
     7441 cctgtaccgc ttggcgaaaa aaatcaagag agcaagtgta tccggttttt cattggttag
     7501 cagtgaaacc agattaccaa ggaaaagggc tcgcaaaagc attgactgcc agagtcttgc
     7561 aagaatttcc taccctccat acggacggac caatctatct tcacacacag acatggagcc
     7621 atcaggcgat tgctctttat aaaaaaatgg gttttacttt tattgctgaa aatctagacg
     7681 gcacacctaa tccagattat gaaaaagtta tgcaggtttt aagtaaatta gataaataac
     7741 gaaatggtga aataagtcct cagtattggg ataaaaaacg aaatagtcat tcctcatatc
     7801 ttactttttt gagttaatac tatcggataa ctaatggcat gccatacatt caaaaaagaa
     7861 ggggcagtga caaaagtatt gtcactgccc cttctcttgt tttttagcat gttttatcgt
     7921 ttgatttttt taggaataaa attcttgatg aacgtaaatt tttggtaatc gtttcgacta
     7981 tagaggtaac caaatacact gaaaacaact gctccaataa aattaacaaa taaatccttc
     8041 atagtatcca tgattcccac gtctaagtaa ccattttgga tgattgtttt atctgtttta
     8101 ccatctgcat tttgactttc aatgattgtc cgagtgatat ctggaacatg atagacagtg
     8161 tttccgtctt tacttaactc cacactattg atttgctgga tgatcgtatc tttttgcata
     8221 tcgaattgca gtaatttatc tgctgaaaat tccacaaact cccacataac accaactgtc
     8281 atcgaaaaac agaaggaaac aactgctagt aataaaggag tcaagttcat ggtttttgta
     8341 tttttattga gcaaataaac aagggaaaag ccgacacccc cagctaaaaa tccgctgaat
     8401 gcatgtagaa aagtatccca cattgaaaaa tagccataaa aatcacttaa ctctcctaaa
     8461 actgttgaac taaaaataaa agataacaaa atgatttcta gcatatttgg aatctgtatc
     8521 tttaatatct tttccagaaa ataagggatt tcaaataaaa ttaatgtcag taataaaagc
     8581 attgtatttc cccagtgttg gttccagacc tgatccacta gcataataac gacgatgata
     8641 cgtaataaaa tatagattcc gtttgtgatt ctacctgcag tttttgtact gttcgtaaaa
     8701 aaggttttca aatgattcat aaaataactc cttcatgtat atccttaatt atactaagaa
     8761 gcgtgtgttt accaaagcta tatgacttaa agaaaaacag agttgatatt gtgaaaaaaa
     8821 tcacttaata tagtataagg agaaaagggg gatacaaatg aaaaaattag ttgctggttt
     8881 attaacagcc gttgcacttc ttgctatgag cgcatgcggc gcagatcaga aaaatacagc
     8941 gcagacaagt tctagtacga aggaatctgc cgaagtatca tcaggcgctt ctgaacaagt
     9001 atatacagat ccaaaagaaa tgaaagaatc ttatgatgtc gtgattattg gagctggtgg
     9061 tgcagggatg gcagcagcta tcgaagcaaa agatgctggg ttgaatccag taattttgga
     9121 aaagatgcct gttgccggag gaaacacatc taaatcatca agcggtatga atgcttcaga
     9181 aacaaaattt caaaaagaag aaggaatcaa agactcgaat gatgccttct atgaagaaac
     9241 gctaaaaggt ggtggaggaa cgaatgataa agaaatgctt cgatactttg tcgatcactc
     9301 tgctgatgcg gtcgattggc tagatcaaaa cggtatcaaa ctggacaact taacgatcac
     9361 tggagggatg agtgaaaaga ggacacatcg tccgtctgat ggctcggcta ttggtggata
     9421 cttagtggag gggttattga ataacgtcca tgagcgggaa atccctattt ttgtcaacgc
     9481 agatgtgaca gacattaaga aaaatgggga aacggtcaat gaagtgactg tacatgttca
     9541 aggagaagaa ccaaaagagg tcaccggaaa agcaatcgta gttaccacag gcggttttgg
     9601 tgccagcaaa gaatttattg aaaaatatcg tcccgattta gctgattata ttacgacaaa
     9661 tcaagaagga tcaactggag atggtatcac catgattgaa aaattaggtg gacaaaccgt
     9721 tgatatggat aaaatacaaa ttcatccaac tgtgcagcag gatgaggggg tcttgattgg
     9781 tgaggttgtt cgcggagaag gagcaatctt ggtcgatcaa gaggggaaaa gatttgtcaa
     9841 tgaaatggat acaagggata aagtatctgc agcaattaca gcattgcctg aaaaatcagc
     9901 ttatttgatt tttgaccaag gcgtgcgtga tcgtgccaca gctattgaat tttatgacca
     9961 aaaaggatat gtcacaactg gaaagacaat ggaagaatta gcagaaaaaa tcgatttacc
    10021 aaaagaaaca ctagcaaaaa cagttgatgg atggaatacg gatgttgcgg agaaaaaaga
    10081 tgctcaattc aaccgtgcca cagccatgga acatgactta tcgtctccga attattatgc
    10141 gatcaaaatc gcaccaggaa tccattacac gatgggcggt gtgaaaatca atacgaacac
    10201 tgaagtatta gataaagaaa accagccaat tagggggtta tatgctgctg gtgaagtcac
    10261 aggtggctta catggaaaca accggatcgg tggaaattca gttgcggaga tcattatttt
    10321 tgggcgacaa gcgggcatac aagcagctcg atttgcttcg gaaaatgaat gaaatagaac
    10381 gtaaataaaa agaggttgtg aaatcagcgt tttgagctaa taccgagggt cagctgattg
    10441 aacactgttt agtgaagagg ttgtgaaaaa g
//
LOCUS       DACDXE010000093        10086 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.093, whole genome shotgun sequence.
ACCESSION   DACDXE010000093 DACDXE010000000
VERSION     DACDXE010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.093"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(247..759)
                     /locus_tag="IWM23_002543"
     CDS             complement(247..759)
                     /locus_tag="IWM23_002543"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAP9885137.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWM23_002544"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="IWM23_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAP9885138.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3646)
                     /locus_tag="IWM23_002545"
     CDS             complement(3212..3646)
                     /locus_tag="IWM23_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAP9885139.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="IWM23_002546"
     CDS             complement(3656..4444)
                     /locus_tag="IWM23_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9885140.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="IWM23_002547"
     CDS             complement(4441..5382)
                     /locus_tag="IWM23_002547"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAP9885141.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="IWM23_002548"
     CDS             complement(5559..6146)
                     /locus_tag="IWM23_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAP9885142.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWM23_002549"
     CDS             complement(6367..7680)
                     /gene="obgE"
                     /locus_tag="IWM23_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAP9885143.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="IWM23_002550"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="IWM23_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAP9885144.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="IWM23_002551"
     CDS             complement(9063..9998)
                     /locus_tag="IWM23_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAP9885145.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DACDXE010000094         9852 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.094, whole genome shotgun sequence.
ACCESSION   DACDXE010000094 DACDXE010000000
VERSION     DACDXE010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9852
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.094"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            23..646
                     /locus_tag="IWM23_002552"
     CDS             23..646
                     /locus_tag="IWM23_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885146.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IWM23_002553"
     CDS             1096..2145
                     /locus_tag="IWM23_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9885147.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IWM23_002554"
     CDS             2142..3023
                     /locus_tag="IWM23_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9885148.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IWM23_002555"
     CDS             3026..3796
                     /locus_tag="IWM23_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAP9885149.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IWM23_002556"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IWM23_002556"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAP9885150.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IWM23_002557"
     CDS             5107..6270
                     /locus_tag="IWM23_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAP9885151.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IWM23_002558"
     CDS             6387..6797
                     /locus_tag="IWM23_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9885152.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IWM23_002559"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IWM23_002559"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9885153.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IWM23_002560"
     CDS             9337..9654
                     /locus_tag="IWM23_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885154.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
BASE COUNT         3432 a         1656 c         2115 g         2649 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatggaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gc
//
LOCUS       DACDXE010000095         9695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.095, whole genome shotgun sequence.
ACCESSION   DACDXE010000095 DACDXE010000000
VERSION     DACDXE010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.095"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(309..731)
                     /locus_tag="IWM23_002561"
     CDS             complement(309..731)
                     /locus_tag="IWM23_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAP9885155.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(721..2895)
                     /locus_tag="IWM23_002562"
     CDS             complement(721..2895)
                     /locus_tag="IWM23_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HAP9885156.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2911..5253)
                     /locus_tag="IWM23_002563"
     CDS             complement(2911..5253)
                     /locus_tag="IWM23_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885157.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5275..7818)
                     /locus_tag="IWM23_002564"
     CDS             complement(5275..7818)
                     /locus_tag="IWM23_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HAP9885158.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7828..8226)
                     /locus_tag="IWM23_002565"
     CDS             complement(7828..8226)
                     /locus_tag="IWM23_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885159.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8307..8591)
                     /locus_tag="IWM23_002566"
     CDS             complement(8307..8591)
                     /locus_tag="IWM23_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885160.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8615..9613)
                     /locus_tag="IWM23_002567"
     CDS             complement(8615..9613)
                     /locus_tag="IWM23_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885161.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
BASE COUNT         2793 a         2051 c         1495 g         3356 t
ORIGIN      
        1 acttattaca tataaccttt aaataatctc taaataaccc tcaaataatc cttactaata
       61 ctgaaacgta aaaaaaataa acggatttca aacaaaatac gtttattttt tctattgatt
      121 ttagctcaaa atcgattcaa ttcgagtgaa atttgcttca attttcaatt ggtttttgtt
      181 caaattttga aacatttttg ctcaaaaaat gagtgaattt gtttcaaaag aaaaacaaat
      241 acgatcgaaa aaaatttgaa ctcataacaa ttttttgttt ttttcaacgt attctctttc
      301 aattttcact aaaaattagg aagaaattga ttcaatgtct tttcaaaaaa gaatgttttt
      361 ctctcaattt ttttgacgtt tctatcatga atcacgagaa aacttgggac acttttaaca
      421 tggtaacgtt gtaaaaaagt ttgaagttct tcttgtcttg gttcttttct tgtttcaatt
      481 cgatatacat tcctagagaa ttttttgtcg tatctttcca agaaagattc aacttcttgg
      541 cattttttac aatcatttct tgctagatac acaaaaaatg attgattcgt tttttgaagt
      601 tctagtactt cttctaaatt actattgatt agttttccat ttgattcttc gttagttttt
      661 atcagtttaa aacaaccaaa taagaggaaa ctaacaataa gtatcctaac aataactttc
      721 ttatttcgca tattgtttct ccaaatttgg tttccgaata tagacgactc gcggtggttc
      781 ctggacgtct tcagctgttc gatacaccac aaattcgcct gttctcaatg ctcgaatgcg
      841 attcggattg aatttaaagc ggtctacgtc tcgtttcgtg cctctttctc ctgtccaatc
      901 aacctttccc atatcagaat aacctgcttc ttgtgtcgtc atttccgtta attcaatgtc
      961 ttcttctgtc cccattaaat ctgcccaata atcaatttct gtcgggtcat ttgtcttccc
     1021 aataaagaac gtattcacat tcccaatcaa ctgttttcga aggacaggat caattctatc
     1081 aatgtccgca agcgtttgag gggcatacgc cgcatgcacc ccagcgcctc gtcccttatt
     1141 gactaagtct ataaggttat catttagata agaagctggt tcgtcaaata ttactaggac
     1201 tttcgagcga ttggctgttt ctcctgcttg tttatacact tctgacgcaa agaaattaat
     1261 atctcccaca agcatttgag ccaatgtccg aataaactca ctataaatca atccgtttaa
     1321 cgagatataa acaatctttc cttctttaac aacttcttga atatctaatt ctctttttcc
     1381 tccagaagta tcaaagagtt caccaagctc actatccaat aacatattga tattggtctg
     1441 taaattctca gaagtcgcat acaaataaaa cgggttcttc ttatgttcgt aacgaacaaa
     1501 caaccgttta aacaacgttt gctcgatttc tgtcatgaga gaaatgtttt cttttaataa
     1561 tagataatac gtttctaagt caacggtttt acgatcatgt tcaacgattc gctcttcact
     1621 taactgttcc acatcggcaa aatcacgttc tgttttgtcc atcgcttcat tctttttcgt
     1681 ttttttcttc gtggttttct tcttttctac gaccactttt tctttaaata aatctggatt
     1741 tttcaactga atacgatccg caaacaaatg taagacgttt cgtggcaata aataatgttg
     1801 taaccaaact agatttcttt caattttatg gctcttttgg aactcgtcta aaagctgaat
     1861 cgttacctgt aataacgctt tggctgctgt cgaataatac acgctttctg tttctgctag
     1921 attggttaat ttatctcgaa tggcaatatc attgccattt tttacaggat tataacgcaa
     1981 atcgccaccg tccacaaaga cttcaatcgg gcgattgtat gcctctgcca cttcttttaa
     2041 cgcttgtaaa gtctctcctg ctcctttccc atcgactaag ataacgggga tacgaaactt
     2101 acacgcatgt tctgctagta attggatcac cgtcgttttt cctgaacctg tagtcgctgg
     2161 aataatcaca tgttggttca attcactaaa gttcatcaca taagggagcc catactcatc
     2221 tgttcctaat agaatcactt tggctaatga agccagttgt ttattccgca aagcaacgtc
     2281 ttttatgaca attgctacct ggtacgcttg ctgatactct gctgtatatt tttgggactc
     2341 ctgaaattta ttcttccgac gttccttgta ttctttacta tttagcaaag ctgtttgccg
     2401 ttctttcttt gaaacaactt ttcttttttc aataaaagac gcatacgcaa tcaaacaact
     2461 tcctgcacct gtcccaccta aaaggagggc aaaaatcgtt tgtccattaa actgcaacgc
     2521 atgagaaaac aagtttaatg gaaataataa gtaacacgac aaataccctc taggaagaac
     2581 aaaaataaaa caaaatagat ttaatagcgt aaaacctaac gcaaaaataa aaaaaagaga
     2641 gcttcttcgt tttaagaagc tctcatactc aaagtcataa atatgtgcac gtgtctcttt
     2701 tttatctatc ataaagacaa tcgctaaagg aatactgcct acaataaaaa atggatatag
     2761 acaaacacct aataaagtga cgataaaata aagttgttga aaccgacttt ttggcgtttc
     2821 tttttcttgt tttcttttgc gatcgacaga aaacatttgc tcaaaatgtg tcggtcgttt
     2881 tggtgcattc atcatactat cgatttcctc ctatcgtttt cgttgtttca tttctcttcg
     2941 ataaatcggt tgtttttctc gttttcgttt catttttgct tgagttgctc gaaactcact
     3001 ccgtgtcttc ggtgcttgtt ttggtgattt tgtttgtttc atggttgagt atgctttaga
     3061 tacggttgaa ggcgctttgg tttgtgctgt tactcgattg actgctgaca tatgttcccg
     3121 aaccccctct ggacgtcgaa cagatttcgg tgtggtagaa cctgttgctc gtggtacatt
     3181 cccttttctt agtggttttt gtaccgcatg tttttgtcct gcaactgtcc gggttttcat
     3241 cgaacttgtt tttgttacgg gagtaagtgc ctccttccga cgacgacttg tttgtccctg
     3301 acgacgtttt cgatcgttca attctgttct aggcgtatgc ttctggccat aagacttgtg
     3361 tttttgcttt ggattcgcta atgacaccac cattgcagaa ccctgattgc gatacggact
     3421 acgagacgca ccttttctac ttctttctct tagactagga ttgccttgca attctcgttg
     3481 taataaatcg gctgtttttc ttgcctgttt ctccattgct gttcgttgca tacgttcttg
     3541 actttgctct tctgcttgtt tcgcttgatc atgcaagtct cttgcttttt gtgccatacg
     3601 ttccatacga tttggtgctt tatgacgagg ttgttgtgcg gattcacgcc gaaaagcact
     3661 tcgtgtggct tgtttcgccg tttgtggcat acgaacgtct gagccacccc cacgttttcg
     3721 ttgacgttct tgtctcttcc gtgcgttttc tttttctttt tgtttctgtg cttttctcgc
     3781 ttctttgcgt tctttcaacc gttgtttggc ttcccttctc aattttgctt ctgccccaaa
     3841 tggatttccc gcaaaagcta acgctcctcg aacacccatt cttccagtaa atagactgcc
     3901 taaaaagcct cggaacaaat aaattcccaa cggcgcaaac aaataaacaa tgatcaagag
     3961 aattttttcc catggatcgt cttgtgtact acttaattgg aacccgtaac taagaaaaga
     4021 agaaaaaagg atacatgcaa aacctgtcat tccttttaaa aacgtcaaaa taccaaaaac
     4081 tttggcatag ttaccaaaca cattcattgt gacaagaaac aatcctaaaa aaagtaagaa
     4141 cggcaacatc ggtactaaca atagttgcat agttccaata atcaatcgaa acatacttaa
     4201 gacaaaataa acaacggttt gaatcagatt cacgacaaaa taaaacaagg caaatgctgt
     4261 atttttccaa ctattgtaat attgtatgtt cttgtttttc agtgtatctg cttcatactc
     4321 tgtgactttg tcatgaattt tctcccctat ttttgtacct aaatcattgt ccaataaaag
     4381 attgattcga tcatattctt ttccagcata tttgactttc ttctcacgaa ttgtgtctac
     4441 attggttgtg ccataattta acaacagata tggttcataa acattactat aaaagacccg
     4501 agccgccatc aatttgcctg ccgtttccac ttttttatcc gctgttacct cttctgtttc
     4561 ttttgtttca taatttgttt cttcttcagg tgttaacact ggattgacct tcacgaacgc
     4621 cgtttccact gtagaatcta aagaaaaaag aacatcaaaa agattcttat ttgtcccaga
     4681 atcttttaat acgcttaacc cagtaaaaat aaggatcgtc ataagaaaga cacggagtgc
     4741 ttgtttcatt cgagcctctc cagcatattt gattgccatc ataattgccg aaaaactgat
     4801 tccaatagtt cctgcaatac ttaacatatt gtccgcaatt ttttgtgtta agttttgaat
     4861 gggttctcga atggcggaaa taatatctaa ttcaaagaga aattcaacca ttgaagcatt
     4921 gaattctcct aacattttta cgccactcca aacaaaattt ttcatcactc ctgcaacatt
     4981 gtacgatagc ttttttttaa ggttcattga ctcatagtca ttggtcatca attcaaacga
     5041 attctttttg taatttttat acaattgatc cacatcagtc gttacttctt ttacttctac
     5101 tttatcgttg gcattctctc cttcagaacg atcggtttca gtgacacccg caccatcatt
     5161 catttgatcg gcagaaacag tagccccttg tagacagaaa aaaagacccc ctaataaaag
     5221 gagcgtccac catttcagag atttttttcg caaaaaaacc acctcttttt tgttttatcg
     5281 ttgtttttca ttttctaaca ttgcttgaat tttttcatct tgattttcag tagaagaaac
     5341 agccaataac caactatcaa acaacacatc aatggcaatc ggttgatttc ttcccaaatg
     5401 atcctggaat aaacaggttc ctgtttgtaa ttgttccatc atttctaaat tggctgaatt
     5461 cacttccata tcaaagaagg acaatagttt ctctcgtgct tctttttgtt tcggacgaaa
     5521 cgcaaatttg taagaaatta actctttttt atcttcttga tcgtaatctt taaacgcttg
     5581 cacaacaagg taaatatccg tattattcgc acgtcctttt cgcaagctat catcaatcaa
     5641 atagcttcct tgtgcggtat cttcataccc ttttgcctcg tcataaataa tcgcagcgtc
     5701 ttcttctttg tttgtagaaa agatatacgt aaacttcata atgacttcca taattgccat
     5761 tcctgcaagt tgttcgctat ttaatcgacc cgaatcaatc tctttttgtg tcggaatgcg
     5821 tattccttgt gtccctaaca cgttgatttg tgaatcaaaa cgtatcggcg tactgtgctc
     5881 attcccaatg agaatctttc ctaaaccaac gctatgccct aaaatcaatt tagctagttc
     5941 tttgtcactt tttcgaattt cttcgataac ttgacttaac gaatgcttac ctggcatggt
     6001 gtaaactaca tcgatggcgt cttggatcac cgttttcttg tggctcgctg tttctcgatt
     6061 ggtatctact tctcctaaca ttcctaacac atttttagcg gtttgaattg cctcttcttt
     6121 tggtaaaaac aatagaggat caagcatccc tcgacagctt tcatctgaag ataacgaaat
     6181 aaaattaatt cgttcataca tagccacaaa ctctggtatg tgtccgaatt tttcaagtgc
     6241 ctttaaaaag aacttttggg tttcattttt cggatcaata taaagaattt tttgacctaa
     6301 aaacatcagc cataaaaaaa tatattttac aaggaccgat tttcctttac ctggtggacc
     6361 aataatcaac gtatttccat tggtatgttc cgcgccttca atacttttct ttgttaaatt
     6421 gggataatac catactagtt tactagatag cgctaacgct tctttcgcag atttaaactt
     6481 ttttgcaata acaatccgtc ctaaaggaaa tcctttctta tttcctactt ctttctttaa
     6541 gtccaagcca taatctgcca caaaacctgt cgtagcgacc tgctcgtaac tacgaaaacg
     6601 ttgcgtactt ccaattaagc attgattaaa caacgttagc tgatccgcta ccgatcgata
     6661 aaatttgaaa tctgtagact caatcgcaaa ttcaatatct ttgactcgtt cttccaattc
     6721 ttcttttgaa gaagcagata caaccagcgt gacggtagaa taacacaatc gtttttcttt
     6781 gcgcttcagc ttatctttta catccattaa ccgttctgta ccaaaattta atacatcgtc
     6841 tgaatctaaa atgccgtcaa cggtctcata ttctttttct tgttcataaa tccgtccagc
     6901 cattttatta atttttcgtt catcagattt tgaatgctca aatcgaagtc gaatatgcgt
     6961 atcaattgga aaagataagc tatcctgaag attttgaata aagctactcc catacatggc
     7021 taaaggagcg tccgtttgac ttaaaaacgt cgaataatgc gtttgattca actgttcaat
     7081 cgcaatgtat cctgcttcat tcgaaatgac gccctcgcaa atttccattg gcgtaagctg
     7141 tctttcaggc aatttttctt ctgatcgatg gaaaaaataa taataaattt gtccaatctc
     7201 ttgggcagat aagcgctcta tcggcttata attggacaat tccgtataga gctccttttc
     7261 taatgtttca tacgttggta acacactact tataggaact cgttgtcccg taatacgata
     7321 gataaacttt ttaaaaacat gcttaagcaa ttgaaaatat tcgatcggat cagacacttt
     7381 catttctgta gtgaaacgca caaacagata cgttcgatac tcgtttaatt gcacttcgtc
     7441 ttttaaaatc tttcctgcct gttcaaagta cactttccca agatctgcgc attctccctt
     7501 taccaaatct cgaatggttt cttcaatgtg cgtatctagg ttaaattgac gtggaatatt
     7561 gataaaatga tagtcatatt catcatgtcc aagtatgcct tcgccatctt gaagatagtt
     7621 cttgaaaaaa tcaatgtcat ttaaaggaaa tacttgggaa gaaagttgat agcctaccca
     7681 cacatcatca tttttggtca aaacaaaatt tcggataatg gcgtctatcg gaaattctaa
     7741 tcgaggaagc ttcgtttttt tctttttgaa ctgccaaatc ccgataataa agacgaccaa
     7801 aagaaacaaa aacgccattt actcgactca ctccttccat ttgtgtaggg aatacgtcac
     7861 tttttcatgg aaaaacggca catacatttc atgttcaaag cgttgtttcc gtttctgata
     7921 ataggacgtt cgcccaatta aatactcgaa aaagtgttta ttgtcccatt tcattgaaaa
     7981 caatgtccaa acgagaacac caatcccaaa aatcataatc aaccaggact ttcgaaacag
     8041 tgtctgcaag aaactaattt gattcacata cgcaatcaaa aataggatca aaagaatgaa
     8101 gagaaatacc ccacctatga ctaattgttc catgcgaatg ccattagaaa tccagggaat
     8161 tttttgtcct ttaatcccgt aaattttgtg agggtgctta aactcctttg tatagttgta
     8221 ttccatgttc actctacctc cttttacaaa aaaagaagcg accagaagtt ctaatcttct
     8281 gatcgctttt gcagtttgtc tttttcttat gataattgta tccagggcac aatcatttgc
     8341 gttaccccca cgttctcttc actatcctta ataaagataa tgttctttag ccattcacca
     8401 atatcaacgg cattcgataa caataagacg agcattatca aaataaagac gccaataatg
     8461 gcaaacgctg cccgcgcaaa gccttgggtc gcataagcac caaccattag aacaatcgca
     8521 acgattgcca atagccaaat gacttgtcca gatacatcat caaataacga tttaagatca
     8581 aaaagtttca tttccattct cctctaagtt cattttattc atttaacgta gtaacaaaat
     8641 aactatcgcc acttgttttt acttccattg aaaagtttga ttggaactcg tttcctttct
     8701 caaaatagaa acgatacttc ccttgcaaaa agtagtttcc ttctttattt tttactgcat
     8761 gatccagttg tacattgatc aattcgccgc cttctaaccc tttttcgtta gaaatcaatg
     8821 ccaatttttc atcatttttg acatataact caaaaaaccg tttcacaaat gcttccagct
     8881 tgatttcttc gctttcagtg accgcttgcc cagaagaata aaatcgattt tcctgatagt
     8941 tcacagaatt ggtattctta ctctcagaaa gattcaaaaa ttgaacgcca tcaagtactt
     9001 ttaaaacatt gttttgataa ctgactggaa aagaaagttg actgcttttc caatgttctc
     9061 cttctgtaaa agtaacctca taaaataggc gatacattgc ttgtgtcaaa tcgatttctt
     9121 ctagtttaat aaagcgaaca tctttcgcta ctcgattggt agccgtcgtt acaaagccta
     9181 gatcagacgc ttccaatcct tgtgctaaat aatgattgac ggaagcctcc cattcgtttc
     9241 gtttttctgg cgaataagta aataaccttt caataaaatg ttgtccctcg tacttcaatt
     9301 gttccgtaga agacaaggtt tggttgactt cagttaacaa tgtttctgga ttgacttgtt
     9361 ttttcactgc taaacgcgtt aatgtatcca ctcgtccaaa gtttaggagg agcacgacac
     9421 ccattaacag aaacaaacaa aaaaaagcgc ctgaaaatac actcatggca cttttttgcg
     9481 aaaattggat cattcgatct gacggttctg ttttcttttt ttggaacaaa aaacgtttct
     9541 tttgtttctt ttccttctgt tgtttcggtg gcttagtttg gaacgcttgt ggattaaatt
     9601 ttgcattact catgagaaat gccataccgt tctaacaata atttcggtat tttgtccaca
     9661 atcactactt cgtggacaac cacacttgcc ccctc
//
LOCUS       DACDXE010000096         9386 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.096, whole genome shotgun sequence.
ACCESSION   DACDXE010000096 DACDXE010000000
VERSION     DACDXE010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.096"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            202..1077
                     /locus_tag="IWM23_002568"
     CDS             202..1077
                     /locus_tag="IWM23_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885162.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="IWM23_002569"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="IWM23_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="IWM23_002570"
     CDS             complement(1642..2973)
                     /locus_tag="IWM23_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9885163.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="IWM23_002571"
     CDS             complement(3122..3835)
                     /locus_tag="IWM23_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9885164.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="IWM23_002572"
     CDS             4010..5434
                     /locus_tag="IWM23_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAP9885165.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="IWM23_002573"
     CDS             5733..6440
                     /locus_tag="IWM23_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAP9885166.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="IWM23_002574"
     CDS             6427..6750
                     /locus_tag="IWM23_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAP9885167.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="IWM23_002575"
     CDS             complement(6839..7876)
                     /locus_tag="IWM23_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9885168.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="IWM23_002576"
     CDS             complement(8197..9180)
                     /locus_tag="IWM23_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAP9885169.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
BASE COUNT         2942 a         1803 c         1600 g         3041 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataag
//
LOCUS       DACDXE010000097         8829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.097, whole genome shotgun sequence.
ACCESSION   DACDXE010000097 DACDXE010000000
VERSION     DACDXE010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.097"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..216)
                     /locus_tag="IWM23_002577"
     CDS             complement(37..216)
                     /locus_tag="IWM23_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885170.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(597..1304)
                     /locus_tag="IWM23_002578"
     CDS             complement(597..1304)
                     /locus_tag="IWM23_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885171.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1297..1734)
                     /locus_tag="IWM23_002579"
     CDS             complement(1297..1734)
                     /locus_tag="IWM23_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885172.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1749..2000)
                     /locus_tag="IWM23_002580"
     CDS             complement(1749..2000)
                     /locus_tag="IWM23_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885173.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2000..2206)
                     /locus_tag="IWM23_002581"
     CDS             complement(2000..2206)
                     /locus_tag="IWM23_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAP9885174.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2643..2903)
                     /locus_tag="IWM23_002582"
     CDS             complement(2643..2903)
                     /locus_tag="IWM23_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885175.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2978..3181
                     /locus_tag="IWM23_002583"
     CDS             2978..3181
                     /locus_tag="IWM23_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAP9885176.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWM23_002584"
     CDS             complement(3322..3807)
                     /gene="ssb"
                     /locus_tag="IWM23_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9885177.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3810..4709)
                     /locus_tag="IWM23_002585"
     CDS             complement(3810..4709)
                     /locus_tag="IWM23_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9885178.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4722..5315)
                     /locus_tag="IWM23_002586"
     CDS             complement(4722..5315)
                     /locus_tag="IWM23_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection protein"
                     /protein_id="HAP9885179.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWM23_002587"
     CDS             complement(5556..7760)
                     /gene="topB"
                     /locus_tag="IWM23_002587"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9885180.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7771..8793)
                     /locus_tag="IWM23_002588"
     CDS             complement(7771..8793)
                     /locus_tag="IWM23_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9885181.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTNETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKVIYEEVAKEEKITPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEAINLKDICREVAKELKACERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIYQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT         2253 a         1823 c         1507 g         3246 t
ORIGIN      
        1 tgctatcact tttagttttt aggataaaaa gtgacgtcac tttttgagtg ctggtacctc
       61 aaatcctgct ttttcttggg caatcaataa atctaacgca atacacataa tcctggattc
      121 acttttgccg tacttctgct ttagttgctg caattctttt tgtctctttt catccaacgt
      181 cacgggtaac cgtttatttc ctattgcaat tggcattagt actctctcct ttcatcgaaa
      241 taaccctatt aaaaatatgg ctgcaatcaa cttgaatatt agactcacaa gggttaacac
      301 aaagcgccaa aagatcaaac aaaataccag tattcctaag aatactacga agtttaaatg
      361 tagagctgca ccatgacgca taagtatctc gccaacaaac agtacgaaca atagaggaat
      421 gattttattc atatggatct ccttctttct cttaggttat cgggctttgc ccgcttattt
      481 cgcaccacct aagcggcggg ggcggttgga tcgattggtg cgaaataaga accattccct
      541 taatttcaag gttttttgtt cttatttcac acatcctcac ttttcggtat aaatggttat
      601 tgaccttttt tcctttcaaa tcgttgatat ggcattcgtt tacggtcaag ttcattttgg
      661 atttcttctg tatcgtcgta agtgtaggtg tctaaaaaat cagttaaagt cgtgtaccag
      721 tcgtcgcctg ggttatctac ttcttctaca tattccctca aaaatgcttc gggtacagtg
      781 agttcataat ccattcccat taacacgata gattccgtcc cacacgtcaa aaatctagta
      841 aatacaaggt aataatcgtc taccgtcgca tctttgctgt ttagctcttc catttcttct
      901 tccattaatt ccatgctctc aaaaattgaa taatattctg attctgagta atagtgtttc
      961 ctgcaaatcg tgtagctgac aaagttttcg accagctcac tcctttctag gtaaacattc
     1021 atgatatatt tttctgtttc tagtcgtaga agctccgctc ctttttttaa tgtgtcagga
     1081 cgacgtttat acacgtctaa acgattatgg ctgactgtaa ccttcgtttg actcgtttca
     1141 ctttccatat acaactgttc ttccactctt tgaataaagt acttcatttc ttccatacaa
     1201 ctaaagtctt tttgaatccg tctgattact tcattcaagt gttctttgtt tgttaaaaat
     1261 tgcgaaacat cttctgttgt tagctgataa accaccttat tcattatctt tctcctccga
     1321 ttcaatcgta taaaatgcac catttttttc gatattttca aaagtatcta gccagtcgct
     1381 agccattgct ttagctttct taatctgtgc gaattttgca taagtcagtt ctaaggcttg
     1441 atattcggaa ggtttgatag tgcttggttt gacacctact tccaagcata gtaaggcttg
     1501 gaaaaaccat tttccatttt gttttttcag ttcttccagt gttcgatagt acgtcgcaat
     1561 gagattcatt tcgacacctg attcaatcat ttcttccact gcgactgaca atttttcctt
     1621 gtaatgtttt ttgggtacac gtgtttcatg gtataggaac gtcatatcct ccattttttg
     1681 tgccatttta tccaaaggta atttgacaaa ttcgtcatag taattttgag ccatattttt
     1741 tctccttttt agttctctaa tttggttaac caatattcga tttcataagt ttccatgttt
     1801 tcttgataat gcagttcata tcctgcaatc ttcactaggt cttcaaatag catagtcggt
     1861 gcatcctcta ctaaaggggt ttctatcgtc cagtaccgcg gaataagtaa agcttctgct
     1921 actttttcag aaatgacttc tttgtccacc aaaatttgaa cgacttcttt tacagtcaat
     1981 aatttgtgtt gtgttttcat caccgtttct ttactttctc atcaatcaaa acataatcgc
     2041 cataagacaa ggtatatttt ttcccgcatg ttggacattg atacaaacga tagcttttga
     2101 tacatttcgc tctttctcca caatcgcatt cgacagtatg tggtgcatga tagtttggga
     2161 ataattcgct gaagggaaca ccccaataat caaatttctt tcgcatgatt gattctgcct
     2221 cctcttagga aaggttttat ttcctttcgt tagttctttc tttattgatt tgttggccag
     2281 aatgaatcaa cgaaaagcga caaggacaag ggcgcgcagc gtgccattta cccttgtcct
     2341 tgtcgctttt tggtgataaa aggcaacttg ttgccggtct ggcaaatcaa taaagagata
     2401 gcgaacagga acgaaccttt tggtgagagg aagttttttc tattttgtct tgctttgcaa
     2461 gacaccatat cttagtgaac gtgcaacgtt cacttccttt cttctcgaac ggcggtggcg
     2521 gttggagtga gagaaaggtt tttcaattga gaaggcactt gtgtcttcct aaaaaggtct
     2581 tttcctgttt ctatgtaaaa caaaaagagc tgcaatcctc atttaggact gcagctcttc
     2641 ttttatatgc ccatcgatcg cgtaaaatga tcggggtttc taatttgcat cgattcaatg
     2701 actaattgat tccgtttatt gaaatgacct gtgacggcaa tattgtattt tccatcatcc
     2761 ataattaaca atttatcgat aatttcaata tctgcaacta cacaattaat cgattcatga
     2821 attgtttgta aagtaaagcg aaccaataat ggttgtgttt ttattactcg gattttatca
     2881 agcaaaccaa catatttgtt catcacgatc acctcgctaa aattatagca aaaacagcta
     2941 aaattgagaa tttttattcg attttagata tgctataatg atcaagacag taaagtttgt
     3001 gacggtggct atccgattga aagtgaggtg gtgcgtatgt gcgtacttcc aaaatcttac
     3061 agaaaggaga agccttttgc ctgttgaaac ggcgctagga ttaatgatta gtttcggttc
     3121 tttgatcgca acaatcattt ttggaatctt agcagttgta aacaacaatc aaaaaaaata
     3181 accgtcagct ttagccggtc gacgattatt tttcaaaaat aatattaagc tggtcaccgt
     3241 cttaaacggt gctgttaggg gagttgttag cgcaactcct cttttcgtat ccattataac
     3301 aactttattg atcttttaca atcagaatgg taaatcatca tcactaatat cgattgattg
     3361 tccgccaaat ggattttgtg aagcgttcaa tccattgttg ttaggcgtag ctacatcccg
     3421 ttgttcaaag gtggtagggc ttggactgct ggacgattga tcagcgtgcg cacggctttc
     3481 tgtcgctgct ttactttcta atagttggaa attatcggcc acaacttctg tcacatacac
     3541 acgttgtcct tgttggttat cgtaggttcg ggtttgaatc cgtccaacaa cgccgatcaa
     3601 actgcccttt ttagctaatg tcgccatcgt ttctgctggt ttgcgccaaa tcacacagtt
     3661 aataaaatca gcttctcgat tgccgttttg attcgtgaaa tttcggttga cagctagggt
     3721 aaaagtcgct actgcagtac cactcgcggt ataacgtaaa tctggatctt tggttaagcg
     3781 tcctactaaa gttacattat tgatcatctt tatctctcca tttcttgatt catttgttgt
     3841 gatcttttaa actgccgttt tctttcttga ttttcaattt tgatcacact tttcattagg
     3901 tgatcaattt cgttgactgt atctgtaaat gaaatggaat tcgcaaacgt gttaactgag
     3961 cgatattttt cccactgctt ttccaaatag tcatttcctc tgatcttatc catttctctt
     4021 tcataataaa aggacaacgg ttttgtatcc ccacgttttg taaatgtatt tcgaacactt
     4081 ttgtataact ctttcgtatc gatattttct agtttcggaa tggtaaataa gtcgtagtaa
     4141 tcttttgctc tcgaattatc tgtgccaaag ctgaacgtag cacttagctt ttctgcaata
     4201 atttgttctg ttggataagc tggaatctcc acttttttat cttcaaacat taattggtga
     4261 tagtgtgtat ctacttcagg ataaatagaa tctcctgttg tcacgtccat tttaaacggt
     4321 acacgtactt tctctaaatt agccaccaat ttaatttgaa atccaggata aaaatcggct
     4381 tctcttgttt ccttcattcc ttgaatagag aaaacaatgc cttcttttgt gggttttcta
     4441 gttaaatcat taaaaatgct cttcaatttt tcttcagtta atttgctatt tctaaatgtc
     4501 gtatcgatat caattgttgt ccttctatcg attccatatt ttgatcctaa taaaaagccg
     4561 cctttcaaaa tgaagtcatt tttgtacttt gattcagaaa tttgttcaat tagacgatcc
     4621 atcataaaat gccgttgcac taactgtgca tttaatcctg tactctttgc taaatttctt
     4681 gtttttgctg caaattgtgt cggtgtcatc tttttcctcc atcaatataa gctttgcatg
     4741 tggtatttca tttctttttc tacatgtagt tttgtcatgt aatctcttaa tttacttgga
     4801 tctctcaatg ggtcttccat gtactctttt aatgcttcta atttcatttc atattcaaat
     4861 tcatatctcg gattccaaat atcacaaagt gtacgctctt tatcatacat agtaatagga
     4921 tttccttgaa aggaagttcc ctctactttt cctagttcat aaacttcttt cttcacgaaa
     4981 tgaaattgta actgctgctc ttttgtaatt gaacgatcaa cgttatagcc atggggaaaa
     5041 gttaagtgaa tgaatttcgg catattcgta gaaagctggt aaatatctaa agtcgtttca
     5101 tgagagaaga ttcccctgga aaatcgttgc tgaagccaaa ataaatcatc tattccatga
     5161 ccatcctcta cataataacc acgatcaact ttgatcaatt ttccttgatt caccaatctt
     5221 tgtacagtgg ttgctgcgat tccaaatttc ctggcttcct tcatagataa atttccatcg
     5281 cgaatagcca acaattcttt cagtttatct tgcatttact tcccctccct tcgttacaaa
     5341 tacactacat caaatatatc atagtagttt atttgtaaca agattaacgt ctatcgatct
     5401 aattcgattt ctttttgaat gcgctgctgg ttctttaatt gttgcagtct tcttttattt
     5461 gcacgtaatt tctcttgttc taaacgaatt tttgcttttt gattttcgat ttcttgttgt
     5521 ttgtgtgttg tatttcgcat aggataaccc cttcattatt ttgtagtgcg gcgtgttctt
     5581 ttcttatagt tcgttttttt cttttcagaa aattcaaagc ctgttgtaaa gtcttctttt
     5641 aaaacgatcg ttgcttcaaa cgaacctttc tgacctttta gtcctctgac tgattggctt
     5701 gttcggccgt tttgaatgat ctctttcagt tgtgtggctg tgagtttctt tttggcaatc
     5761 gttgtccata atttaaaatc acaggcttca gacgtacatg ccgccacatt ttgataaagc
     5821 aagacttctt gttcccgaca ttttggacaa acacctaaat gacgcgttgc ttcctgtttt
     5881 tcactggctg ctttcacctc ttgtaacgtt gatccaaaat ctgttgactg gatttgtccc
     5941 ggtacgactt ccaacaagtg agagacaaat ttttggatat tagctaagaa agtagcttgt
     6001 gttcctttct gctcaccaat ctttaataag taactttccc atttggcggt catttcggca
     6061 cttgttagca gcggctcttt ttccacggct tggcaaagta aaatcccttt atttgtcacc
     6121 acaagcttat ttttttctac cttgatatat tccttttgtt tcaaagtttc aataatattt
     6181 gctcgtgttg cttctgtacc gattccttct acttctttca atatggattg tgcttcttcg
     6241 ctatccacgg tttttccagc tgttttcatc gctgtgatca acgtaccttc ggtataaggt
     6301 tgtggtggct tcgtttcttt ttcaaggact tgtagatcta ccgtcaccgc ttctcctttt
     6361 gtcactttag gaagtagcgg ttcttcttcc ttttcttttc tgtcgctctc tttgccaaac
     6421 agctttttcc aacctagatc tactggtact tttccgactg tctttagctg aagctgctga
     6481 acttgcgtca agatcgttgt ttcttcatag gtatactttt ccgcaaacat ggccaccgtt
     6541 gtttttaaca cttgttcata aatagcctgc tgcagtggac ttaattgttc aaaagccgct
     6601 gccgtcatga ccgtctttgt aggaatgatc gcgtaatgtt cctggacttt cttattattc
     6661 acatatcgac tattcggtgt atgaatagga gtaggaatgg cttcggcctt taaaaagtgt
     6721 ttgtattcgt ctaagtgatc caacagatac gcatactccc cttctgtaat ataaggggta
     6781 tctgttcgtg ggtagcttag atatttccct tcatacaacc cttgcatggc ttccaaagta
     6841 tctttggcac tagctttcat gagctgattc attttagatt gaaggctgga tagagaaaaa
     6901 agacgtggac ttgccgcact ttttttctcg gtttcaactt ggtggatcgt acctggctgt
     6961 tttcccaatt gaaccccctt ttcttttaaa taatcttcaa atgcttcttg cgtcgcttgg
     7021 gtttcattcg gatcaagctt cgcatcaaat gcgccatttt gagcgattac ttgggctttt
     7081 ccttcaaaat atggttcttt ttggaagttt ctaatctttt cctgtaattg ataaatcatg
     7141 taaagcgttg gggtttggac tctgcctaag gaaaaacttc ctggtatgcc tttttgctgc
     7201 aagagtaaac tatacagcgg gctaccgttc atgccgatca accaatcggc gatctggcgt
     7261 gcttgtgctt cttcaaattt tgggtaggtc tcttctgcat ctcgtaactg ctggaatccc
     7321 tgataaatgg cttctttttc taaggagttg atccaaagtc ttttaaatat ctttccatga
     7381 tcggcgcctg cttgttgaat gatcgtccaa gctatcagtt ccccttcacg atcgctatct
     7441 gtcgcaataa taatggtgtc ggccgattga agttgctgtt tcaccacctt gaattgcttt
     7501 ttcttatctt taggaacatg atagtgataa tgtgtaggga aaataggcaa atgctctaat
     7561 gaccattgtt tccaattttc atcgtacata tcgggcgaat ctaactcgac taaatgacca
     7621 aaaccatacg tgatcacagt ttctcctgaa aacaatcgat cttggatctc aaaataccca
     7681 tcttttcggg tagctttaga aaagctatcg gcataagcct tcgcttgtga cggtttttct
     7741 gctaaaataa cggttttcat catgacgatt tcactccctt tcttgctgtc taccttgttg
     7801 aaacaacatg gcacgttttt gctttctgat ctcttgttgg tagatttcat gcttcaattg
     7861 atcattccaa tcctttttta cttcaggtac aaattcatgt tcatgatttt tatagccttt
     7921 gtagtattgt tctgctttat aagagaaaag aatctgagca ttaatatccc ctttttcttg
     7981 ataaaaacga tcaaaatcgt acgtaccatc cacgcgttca caagctttta attcttttgc
     8041 gacttctcta caaatatctt tcaaattaat agcttctatt gaatcgttaa cagaaagctg
     8101 ctgaccaaaa gacgcattta ctttgtgagc ggaaaccaat tggttcgtta attgcagatt
     8161 gttttctgtc ttgtgtatgg ccataatagc ttctggtgtg atcttttctt cttttgcgac
     8221 ttcttcataa atgactttgt attcttttgg cacttgccaa tcattagcga ttcctcgttc
     8281 acaacgtact ttctgattcg taaatggatc aactgcgccc attaattgtt ctttttcgta
     8341 gaaaatatgt cctgcacggt cattatccac acaaatctcg atggattgag gaaaggcttg
     8401 tttctttctc aattcagata ctgcctcacc aaaataatga ctgatcacat ggtgttttaa
     8461 cccttccatc gaaactaacc acgtatcgtt taactgatca cgatttagtg cagcatagct
     8521 aagtaaatca atactactct caaaaaattt taactgctta gggtcaccga tctttaggtt
     8581 aaagccatga ttcgctgtcg aatttttatc tatatgctta tatgtgcctc tctcaccaaa
     8641 acgttcataa tcaatgaacg ttccttgata actcgcccct actgtctcat ttgtctgtgg
     8701 atctttccat aaaaaggcga ccgcatgatc taaacgatcg tctcgaacta agaaattttg
     8761 ccgttcatgt tttggcttta gttcggcaat caacccttct ttttctgcat aatcaatcag
     8821 ccactctgg
//
LOCUS       DACDXE010000098         8573 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.098, whole genome shotgun sequence.
ACCESSION   DACDXE010000098 DACDXE010000000
VERSION     DACDXE010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.098"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..777
                     /gene="menA"
                     /locus_tag="IWM23_002589"
     CDS             <1..777
                     /gene="menA"
                     /locus_tag="IWM23_002589"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9885182.1"
                     /translation="NYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVI
                     GVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYV
                     NTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDI
                     ENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHL
                     DQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1591..2937)
                     /gene="gor"
                     /locus_tag="IWM23_002590"
     CDS             complement(1591..2937)
                     /gene="gor"
                     /locus_tag="IWM23_002590"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAP9885183.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3071..4312)
                     /locus_tag="IWM23_002591"
     CDS             complement(3071..4312)
                     /locus_tag="IWM23_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9885184.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(4330..5253)
                     /locus_tag="IWM23_002592"
     CDS             complement(4330..5253)
                     /locus_tag="IWM23_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAP9885185.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5474..7966)
                     /locus_tag="IWM23_002593"
     CDS             complement(5474..7966)
                     /locus_tag="IWM23_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9885186.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7973..8440)
                     /locus_tag="IWM23_002594"
     CDS             complement(7973..8440)
                     /locus_tag="IWM23_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAP9885187.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         2355 a         1814 c         1422 g         2982 t
ORIGIN      
        1 aattacatgg attatgaaaa ggctcattct gaagtatata agtatgaaga aaatgtcatt
       61 ggccgcagaa atctatctcc tgtattgatt cgaaacatga ttttagctat gattgcattt
      121 gtttttgtta tcggcgttat tctaacgatc aatacgggtt ggcttttact tttgatggga
      181 tttatctgtt gttttattgg agtattctat acattcggac cgattccttt atctcgaatg
      241 cctttagggg aaattttcag tggggtgact atgggccttg gaatttttgc catggtcgtt
      301 tatgtcaata cattcggttc agatgtctat gatctgacag tcaacttttc atctggacaa
      361 tttgatttgg taggaaatct ttgggctata gcagcaatcg tattagcttc cttacccttg
      421 gtgtttacga ttgccaatat catgttggct aataatttgc gggatcttga ccgtgatatt
      481 gaaaatcatc gctatacact agttttttat atcggacgtc ctgcgggaac agtcttattc
      541 cagctattga tgtatgcttg ctatttcgtg attttaatgg ggttgttgag cggtatctat
      601 gaatggccga tcttggtcac cttcttgacc ttgccaattg tgaagaagca tttggatcaa
      661 ttcaagcaaa cgttacccca gcctatcagt ttcagctatt ccatcaaaaa tatggtctta
      721 ttcaatgcca gttatgtatt ggggctatta ctaacaatca ttatgcaagc catttaacag
      781 catgaagagg ttgtgaaaaa actgccctgc atcaagtaat aagaacagcc aaataaaaat
      841 agctccttat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
      901 gtttatcagg aggttgtgaa aaagtcgctt agtttcgagt attaagaaaa attttgtgaa
      961 aatggctttc cacatttttg cataaatttg gcttaatacc gagaaactgg cttttgaaca
     1021 ccgtttatca gaggttgtaa aatcagtgtt ttgtcctgag tattagagaa acaaatggaa
     1081 aaatagttcc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
     1141 ctatttgttc ggaaaaagtg actaactatg acacaatttt tttcacagtc ccttttttca
     1201 cttgatttga ttcgtaccag cgaacttgac gagaactttc ttggtttggt aactatatgg
     1261 tattctttta acatcctcgt ttgcttcttc taatttattt atacaaagtc acactttttt
     1321 aaacatctgt tcttcgatac atttctattt cttttaaaca tcgataccaa tattttgaac
     1381 ttaagagtgc ataccaaatg gattcattta ctcttcgtca gttacgaaga taatcaaaat
     1441 ctccaacttc cctacttatt tcgttttgag aattatcgaa gcaatcttac ataaataact
     1501 aaaaaacaaa aaaccagaaa atcagtatac tgattttctg gttttacatg gtttgttcaa
     1561 taaaaagctg ttggaactgt taatattaaa ttatcgcatc gttacgaatt cttccgctcc
     1621 tgttggatgg atggctactg tattgtcaaa gtcttcttta gttgcaccca tcttgatagc
     1681 cacggcaaaa ccttgcagca tctcatcgac accaacacca atagcatgta agccgacgat
     1741 tctttcttct tttccgacac agatcaattt catttcgcat ttttggcgat aatcatttaa
     1801 tgcaaagtac attggggtaa agcgtgaacg ataaactttc aattcatttt cgccatattt
     1861 ttccaatgct tcttcttctg tcaaaccaat tgttgcaatt ggtggatgcg taaagacgac
     1921 tgtcggaaca agtttatagt caagataaga atctttcttt ccattaaata aacgttctga
     1981 taaacgtcta ccagctgcga ttgcaacagg agtcagatct aatttaccga tcacatcacc
     2041 aacagcaaaa atatgttcat ccgttgtatt ttggtattta tctactttga taaacccttt
     2101 tttgtccaat tccacatttg tattttccag acctaatttt tcggtattcg gtacacgtcc
     2161 accggcaaaa agtacacaat caccagttaa ggtttcacca ttttcaaata gcaccgtata
     2221 ttcgtcattg tttttagtaa tttctttagc aatatgatgt gcgtaagttt ttattccagc
     2281 ttcttggtac atttggatca aattgtccga aagcatttta tcaaacgttc taagtggtcg
     2341 ttcttttcga tatgcccaca tgacttcact acctaaacca cgaaatacac ctgaaagttc
     2401 agccgcaatg tatccagcac ctaaaacgat tgctctctta ggtaactctg ttaattcaaa
     2461 aaatccattt gaatcaattg cgtattctcc accaggaatg gtcattttac ttgcacggcc
     2521 tccagtagcg atcaagattt taggagcacg ataggtttca ccgtttactt cgatcgtttg
     2581 actatcgata aaagtagcgt aaccttttat agcctcgaca tgattgctat ctaatcctct
     2641 ttgataagca ccgtgtagaa aatcaatata tttttctcgt ctttctacta gaccggcgaa
     2701 atcaacattt tttaaatctg cttggatacc gtaacttgag gcatctcgtt caattgtttc
     2761 caaaatcgta cttgcttgcc acataacttt tttaggtaca caaccgacgt tgacacatgt
     2821 tccgcccaat tcattcgctt caatcagcaa aacttttgcg ccatgcatcc ctgctctgtt
     2881 tgctgaagca ataccgccag agccgccacc aattacgatg taatcatata tttgcatttt
     2941 gttcctcctt attgaagaga tcaatcaaaa cctttattta acgatccttt ttctacagaa
     3001 aacaagtgta acacttttct tgaattttgt atgcgaagat gcttaaaaca gggatatatc
     3061 agctttttcc ttatttacgt tttcggatca tatctccagg tttcaccggc tgtgcgactg
     3121 gcatagttaa aatcatcatt ggatttggtg ctcggtcaat ggattcgcct tcttcattat
     3181 gcataacttc aacggtttga ctgaaatgac tgaatcctgg accataaaat tcgatttcat
     3241 ctcctacaga aaacaagttt ctctgacgta tcgtagcaat ctgtgttgcc tcatcataag
     3301 ataatacttc accgacaaat ttatatactg ggattttacg acgttcaccg aataattgtt
     3361 cgttttcagt tggtgtattg tagtaaaaac ctgtagctaa ttctcgttgt gctactttcc
     3421 ataactcgtc gatccattct tgcttgcaga catagttttc aggatcttcc atataactgt
     3481 ctacagccgc tttatagaca tttgacaccg tagatacata atggatcgat ttcattcttc
     3541 cttcgatctt gaagctgtct acgccgtttt caatcaattc tggaatatgt tcgatcattg
     3601 acatatccac tgcactcatc gagaattctt cttccacttc gcctgtatca gttaaactgt
     3661 ttcgttcttg accaaaaggc atatcgaaca atccatactt ccagcgacaa gattgagaac
     3721 atccgccacg gtttgcatcc cgcatcgaca tatggttgga caatgtgcat cgaccagaat
     3781 aagagataca catggcacca tggataaatg cttcgatttc ggtatccgta tttttacgaa
     3841 tctctgccac ttcctccatt gaaacttcac gagctaaaac gacacgttcc aacccttctt
     3901 ctttccagaa ttccaatgtc tcaaaattag ttgctgaagc ttgagtggat aaatggatcg
     3961 gcagacctgg ggcttctgtc gcacaaattt cgatcaaagc tggatcagaa acaatcactg
     4021 cagaaatacc gacatcacgc aaactgcgga aaaattcccc tgctccatct tcatttcctt
     4081 catgggtgac catattagca gcaacgtata ccttcgcatc atgttctttt gcaaaagcaa
     4141 ctccttcagc catttcttca taagtaaaat tacctgcacg gcttcttaaa ccatatgcat
     4201 tcccgccaat atatactgca tctgctccat aatggatcgc ggtttttaac ttttccagtg
     4261 ttcctgctgg cgctaggatt tctggtcgtt tcaattttct ttttactgtc atttctttca
     4321 tcatactcct tatttgatag cagaaggatc aatataaaag aatcctgtat ctaattcacg
     4381 gttttcagga tgcaatttac tgatttcgct agtcattttt tccgcttttt cttgatccca
     4441 gctaccttct aaaatttgtt tttttgcttc ggcgaatagt cgagtgatgt ttacgaattt
     4501 ttcgcctgga gtaaataatc cgtccaattt ccatgtacag taatgatgct gataaagttc
     4561 attcaattca ttcgagagat tcagatcatt gctggcaaaa atatgtgtgc catgactatc
     4621 ttcataaata gaataatgtg tctcttcttt tttaggttcc gagataaaaa gacctctttc
     4681 acgatctttt tgttcgtctt gtttcgtata gttatagtaa ttctgtagca gtggacgttt
     4741 ggattgatgg atgcatgtag caccatacac aagggtttct acaggaatat cgagtttttc
     4801 ttccatcgcc ttcatctctt caaaaggaac ttcacgagca agtactgctc caactgcgcc
     4861 ttttttcgcc cagaaattga tttgacgtgc gctcgttact aatgtttcag catcataaat
     4921 atacggtata cgcaattcag gatttttttt catcgtataa ataatacctg tatcaccaac
     4981 tgtgatcaca tctacgttta tttcttttaa aaaagccaaa tattcaggta catttttcat
     5041 tttttcagga tgaatgatcc catttacagc aatattcgct tttttaccat acttgtgtgc
     5101 taattctacc aattgtcgct gttcttctcg tgtaaatgaa gcaggcagac gcaatccaaa
     5161 ttgctcttca ccgaaatata gtgtatctgc acctgcagca agcaactgtt ctgcttgttc
     5221 aatagtttct actgtggtta tgagttcgat cataattttc tcctcctaca acttcataat
     5281 agtagcatag ctctaaaaac tttccaatac ttcttctatt taaagtcgga tttttgaaaa
     5341 ttatttcaca aaaaatcaaa ataaagttcg atatattctg tccaaatata aaaagagatt
     5401 gtgacaaaaa tcttgtcaca acctcttaat caagtcttac tattataaaa attcagctac
     5461 acaggccaag taactatagg gaacctactg tttccttttt aggtccgcga acattcaatg
     5521 tgatcttacc ttttgaagca cctactgtta cgtggtcacc taaacgaatt tctccgccaa
     5581 gcaaagcctc gctcaatcga tcttcaattt ctttttgaag tgcgcgacgg attggacgtg
     5641 caccatattc aggatcaaat ccagctttcc cgatcacatc gattgcagat ggtgtgattt
     5701 tcacttgaat atcttgttct ttcaatcgtt tgataatcgc tttgctcatg atttggacga
     5761 ttgtatggat ttcatcttta ctcaatgaat ggaagacgac agtctcatcg attcgattca
     5821 agaattcagg tcggaaagct ttctttaact cttctaagat acgtttttgc atggctttgt
     5881 gatctttagt aacatcttgg acattgaatc cgacattttt ttcttctcga atttgtgtcg
     5941 ctccgatatt tgaagtcata atcatgattg tatttctaaa gtccactttt ctacctttag
     6001 aatctgttaa atgaccatca tccaatactt gcagaagtaa attgaaaacg tctggatgtg
     6061 ctttctccac ttcatctaag agaatcactg aatatggttt ttgacgaatt ttttctgtca
     6121 actgtccacc ttcatcatac cctacgtatc ctggaggaga cccgattaat cggcttgtac
     6181 tgtatttttc cataaattct gacatatcaa cacggatcag ggcatcttca cttccaaaca
     6241 tgacttctga aagtgctttg gccaattctg tttttccgac accagttggt ccaaggaaca
     6301 tgaatgaacc aattggacga tcaggatctt tcaatccgct tcgtgcacgg cggatcgcac
     6361 gtgaaactgc tttgacagcc tcatcttgac caacgacacg ttcatgcaga agtccttcca
     6421 gttctaacaa gcgttcgctt tcttttttct ccaactgctg tagagggaca ccagtccact
     6481 ccgatacaac tgtagcaaca tcttcttctg ttacacgatc agaataacct tgctcctgtt
     6541 ttgcttcaat tgcagaaact ttttgcagct ttttacgtag tgctttttct tgtctgcgta
     6601 attgagcagc attttcaaag tcttgttttt ggatcgctgc ttccttttct tgtactagtt
     6661 catcgatttc tagtttgatc acagttgatt ttgttaagtg atcggtttga tccaaacgga
     6721 ctttcgctgc tgattcatcg atcaaatcaa ttgctttatc gggtagttga cgagaagtga
     6781 tataacgaac agaaagattg actgctgctc gtacagcttc atctgtgatc tccactccat
     6841 ggtgctcttc atatcttgta cgtaatcctt gaagaatcac ttctgcttct tcaggagttg
     6901 gctcatctac ttgaatgcga gcaaaacgtc gctctaatgc agaatctttt tcgatatatt
     6961 tttgatattc atctagggtc gttgctccga tcgtttgtaa ttctcctcga gctaacgccg
     7021 gtttcaagat attcgaagca tcaattgctc cttctgctcc acctgcacca attaaggtat
     7081 gcaattcatc aatgaataag attacttgac catcattata gatttcgtcg atgacctttt
     7141 tcattcgatc ttcaaattca ccgcgatatt tcgtaccagc aaccaaagat cccatatcaa
     7201 gcatcatcag gcgcttttct tgcatgtctt ctggcacttc acctaaaatg attttttgag
     7261 ctaacccttc agcaatggct gttttgccta caccaggttc acccaccagt acagggttat
     7321 tctttgtccg tcggctaaga atctggataa gccgtttcac ttctctcgaa cgaccaacaa
     7381 ctgggtccag cttgttttct tttgctaatt tcgtcaaatc acgtgctaat gaatctagcg
     7441 taggtgttcc ttgcggtgtt tgccgtttat cttgtacatt tacatttcta cgacgattca
     7501 ttccttttcc agcagaatca ccagctagcc ccattttttg tttcagaact tgacgggctt
     7561 ttgaaaggct gatacctaag ttgatcatga ttcgagtagc catgatactt tcatctttta
     7621 ataagctcat caaaatatgt tctgttccaa ctttttgaga accagttttc ttcacttcgc
     7681 tgctagctaa agcaaaaact tgtttcattc ttggtgaata tggaagaaag actcctttag
     7741 ggtaagaacg gataccacca taaccaatca gatgttcaat ttcttcataa atttccgttt
     7801 cattcaaatc catttcacgt aatacttttc cagcaattcc attttgctcg accactaagg
     7861 ctagtaaaac atgttctgaa ctaactgatt gatgtttcaa ccgttttgct tcttcttgtg
     7921 caatttctaa tactgcttgg gctctttcag taaaaagttc ttccatctaa tctcacttcc
     7981 catcttcata acttaaacgt tccagaaatg ctcggagaat ccgagcacgt gtatgatctt
     8041 catattcatt gaaattcaat gtgttttttg caattgcact caacatcaag tttccttctt
     8101 tttttgtaat tatttggtct tcataaagtt tttgaataat agcaaaagca tttttctcac
     8161 tgattgtatc atcaaataac tgatttatct gctcaagtaa ttgtttttta tctgaaatcc
     8221 ttacttttgc aattcgtata taaccaccac caccacgttt gctttctact agataaccac
     8281 gttggatggt aaaacgggta ttaatcacat agttgatttg tgaaggaaca cagttaaaga
     8341 ggtctgccat ttcaatcctt cgaatctcga tcatttcact attttccaga attttcttta
     8401 tatatgattc aattaaatcc gaggtattct gatgtgccat cttattcatt ccctttgact
     8461 aatattgacc ttaattataa cgaatttgat aatagaaaga aagaagaacg ctttttgtta
     8521 aataaatcct ttagaatcaa caaaaaaaaa tacggaatat actattccgt atc
//
LOCUS       DACDXE010000099         8493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.099, whole genome shotgun sequence.
ACCESSION   DACDXE010000099 DACDXE010000000
VERSION     DACDXE010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.099"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..173)
                     /locus_tag="IWM23_002595"
     CDS             complement(<1..173)
                     /locus_tag="IWM23_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009325336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885188.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="IWM23_002596"
     CDS             complement(593..1714)
                     /locus_tag="IWM23_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HAP9885189.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /locus_tag="IWM23_002597"
     CDS             2042..3451
                     /locus_tag="IWM23_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAP9885190.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /locus_tag="IWM23_002598"
     CDS             3448..4176
                     /locus_tag="IWM23_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885191.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="IWM23_002599"
     CDS             complement(4304..5215)
                     /locus_tag="IWM23_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885192.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="IWM23_002600"
     CDS             complement(5232..6239)
                     /locus_tag="IWM23_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAP9885193.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="IWM23_002601"
     CDS             complement(6236..8365)
                     /locus_tag="IWM23_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAP9885194.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..8477)
                     /locus_tag="IWM23_002602"
     CDS             complement(8370..8477)
                     /locus_tag="IWM23_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885195.1"
                     /translation="MQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2680 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg ttt
//
LOCUS       DACDXE010000100         8414 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.100, whole genome shotgun sequence.
ACCESSION   DACDXE010000100 DACDXE010000000
VERSION     DACDXE010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.100"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(19..2628)
                     /gene="clpB"
                     /locus_tag="IWM23_002603"
     CDS             complement(19..2628)
                     /gene="clpB"
                     /locus_tag="IWM23_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAP9885196.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQPNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(2879..3925)
                     /locus_tag="IWM23_002604"
     CDS             complement(2879..3925)
                     /locus_tag="IWM23_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAP9885197.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            complement(3936..4208)
                     /locus_tag="IWM23_002605"
     CDS             complement(3936..4208)
                     /locus_tag="IWM23_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAP9885198.1"
                     /translation="MDVLLHNLLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            complement(4243..5151)
                     /locus_tag="IWM23_002606"
     CDS             complement(4243..5151)
                     /locus_tag="IWM23_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAP9885199.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            complement(5283..6515)
                     /gene="pepT"
                     /locus_tag="IWM23_002607"
     CDS             complement(5283..6515)
                     /gene="pepT"
                     /locus_tag="IWM23_002607"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAP9885200.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            complement(6530..7651)
                     /locus_tag="IWM23_002608"
     CDS             complement(6530..7651)
                     /locus_tag="IWM23_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAP9885201.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            complement(7648..8349)
                     /locus_tag="IWM23_002609"
     CDS             complement(7648..8349)
                     /locus_tag="IWM23_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAP9885202.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
BASE COUNT         2318 a         1835 c         1448 g         2813 t
ORIGIN      
        1 tatagtctca atggaagatt agacgatttc ttctagctct tctgttttga aatgaagttg
       61 tccatcaagt aaagtaatcg ttacttttga tttaggcata acatgtcctg caacgatttc
      121 tttagctagt ggtgtttcta cttccttagt gataaatcgt ttcaatggtc ttgcaccata
      181 tgctggttca taagcatttt cagcaatcca cgttttggct tcatcactga ttgtcagcag
      241 gatttcttga tgttccagac gttgtgccaa ttgcgccacc attttgtcaa cgattccttt
      301 cacgttgtct aggctcaatg gtgtaaataa aatcgtgtcg tcgattcggt tcaagaattc
      361 tggtttgaag tttccacgta atagcgtgtt gacttgttca gccactgctt ctggaatcgt
      421 tccatcagca gtcacgcctt ccagcagtaa ctgtgaaccg atattgctgg tcatgatcag
      481 aaccgtattt ttgaaatcta ctactcgccc ttttgaatca gtcaagcgtc cgtcatctag
      541 gacttgtaac aagatattga agacatcggg atgcgctttt tcgatttcat ccaacaagac
      601 gattgtataa ggatttcgtc tgacagcttc cgttaattga ccaccttctt cgtaacctac
      661 atagcctgga ggagctccga ctagacgaga cacagcatgt ttttccatat attcactcat
      721 atcgatccgt accatatgat cttctgagtc aaacaagttc tcagctaacg ctttcgctaa
      781 ttcggttttt ccgacaccag ttggtcctag gaaaaggaaa gaacctagag gacgatttgg
      841 gtcttgcaat ccagcccgtg aacgaattac cgcgtcactg accgcatcaa ctgcttcatc
      901 ttgaccaatc actcgtttat gaagtgtttc attcaattta attagttttt ctcgttcacc
      961 ttctacaagt ttcgttacgg ggatacccgt taatctgcca acgacttgag caatctcatt
     1021 ttcggtaacg gactcttgaa ccatcttaat ctcgctgtct tttgctttag cttctaattc
     1081 cttcaattct ttttctaatt gaggtatcgt accatgacgc aatacagcag cgcgttctaa
     1141 atcatagtta ttttctgcat cttctaattc gtgttttgct ttatcgattt cagcacgttt
     1201 tgctgaaaca gaattgactt cttctttttc tgtttcccac tgcatcttca tcgcatttgc
     1261 ttcttcacga agttcagcta attcttcttg caaatttttc aagcgttttt tacttgcatc
     1321 atctgattct tttttcagtg cagcttcttc gatttccaac tgcatcaaac gtctagtaac
     1381 ttgatctaat tccgttggca tagagttcat ttctacccga atcgttgcac tggcttcatc
     1441 gatcaaatcg attgctttgt ccggtaagaa ccgatcagta atataacggt cagataaagt
     1501 agctgctgct actaatgcgt tgtcatggat gttaacacca tggtggatct caaaacgttc
     1561 ttttaatcca cgcagaatac tgatcgtatc ttccactgtt ggttctttga ctaatacctt
     1621 ttgaaagcga cgttccaacg ctttgtcttt ttccatatat tgacggtact catctaaagt
     1681 tgtggcaccg atcaaatgta attcaccgcg agcaagcatt ggtttcaaca gatttccggc
     1741 atccatactt ccttctgttt ttcctgcccc tacgatattg tggatttcat cgatgaacaa
     1801 gaggatacgt ccatcacttt ttttgacttc tttcaagact gcttttagtc tttcttcaaa
     1861 ttcaccacgg aattttgctc cagcgatcaa tgcacccata tctaatgaaa aaatcgtttt
     1921 atcttttaaa ttttcaggca catctttgcg tacgatacgt tgagcaagcc cttcaacgat
     1981 tgccgttttc cctacaccgg gttctcctat cagaacagga ttattttttg tttttcgtga
     2041 aagaatacgg atgacatcac ggatttcttc atcacgaccg atgattggat ccatttttcc
     2101 gcttttgact tgctgtacta aatcaacgcc gtatttttct aatgctttgt attgttcttc
     2161 ttggttttgt gaggtcactc tttctcctcc tctcatctct tcgatgtttt tacgcaattc
     2221 tttttcagat aatccgtttt tattcaaata aacagttaac ggataatttt ttaatttcat
     2281 caacgctaaa ataacgactt ccgttgaaag aaactcgtcg ccgaagcttt cacgtatttg
     2341 gtctgcttca ttcaataatc gaaagaaatt ctggctgagg ttttgtccat attggacgtt
     2401 tccaccagaa acaactggat actcatccag tagacgatca atttcatgtt caaaagattc
     2461 gacatccaat ccggcatccg tatagaaatt tcgaccaaaa tgattaggtt gtaaaaagat
     2521 tttccataca tgggcaatgt cgatatcttg atgttttcga gtgaccgcaa tctgttgagc
     2581 ttcagcaatc gcttcttgaa gcgtcgtagt cattttctca atattcatac aggtataccc
     2641 tcctaattaa aggtaagatc acttatttct ttgatcttta caaaaactat tatatcgctt
     2701 tggtcaattt tggtcaaaat atacacatag ttttttgact ttttttctcg acttatataa
     2761 atcttaaaaa aacaaaattg attgtatgta tataaaaaaa gagccaaaca aacgtaaaat
     2821 tatcttatcc ataacgtttt tctttgattt gataaaaagg gtagatctta taaaagacct
     2881 acccttgatt ttctttattc tcttttttta cttctattat ttcttctttg atcactcttc
     2941 gccgttcagc aatcacatac acaaatccat agagaaatgg caagataaag gaactgaaac
     3001 gatacagtaa aagcgaagaa actgcgtcaa ctgttccaac aactggtctg aataataaca
     3061 aatagacaaa ttcaaaggaa ccaataccag caggcgtagg aataacccct gaaagcacta
     3121 ctgcaaagct gataaacgaa atggtcagta gaaaatctat tttaggatgg ttttcaatca
     3181 gaatcaaata aggaagtaca taccaaaata caagttttaa catattccat agataaatcc
     3241 gcaatagcgc agtcctgtct tggataatgg tttggaccgt ttcccttaaa gagtagatct
     3301 gctgattgca tgtatccacc catttccgca aacgctcact tttaaataac ttattcgata
     3361 agctgacaaa aaatatttga acattcaagc tgacggacat cgccaataaa aaggcaacaa
     3421 tcacgatcgt caaaaggata ccagcgatga taaaccagat cattgatgga ccattagaat
     3481 aaaaaagaga gaattggatg accaagccag ctaatgcata agtgatcact gccagtttgt
     3541 acataatcat atggagtgct gtcacccctg ctccctggga tagcttcatt ccttttttgt
     3601 tataaaaata aatctccgag atcagcgttc ctgtaccaaa cgaaacgatt cggtaaaacg
     3661 caatatagct agaggtcaag aacccatctt tcgttgtaaa attcggcgcg aaaggttcag
     3721 caatttcttt gattgagcgc ccttcagcga tttggtaaac tgtccctaat ataacagccg
     3781 ctgccaaaac aacaacactg gtagacagca attcggcaaa gatatctgca aatgattgat
     3841 tgattaaata ataaataatg gcaaaaatca caatcagcaa aagcagattc agaatgattt
     3901 tagttttcgt tcctttagac gacatctcct gctccttaat ggaaaaaata aatgagaatc
     3961 aaatcaacaa gtacacctgc agcgaatcct atagtaaact tctttttccg agttttatga
     4021 tggaaaactt ttcgagagat caatcctccc aaaccaccgc ctaacaggct gacaaatatc
     4081 aaattattct caggaacacg ccagccgcta gttctagcct gatgtttatc ataccccatc
     4141 agacaaaata agtaaaaatt cacaatgaaa aggtaaaacc acaaaagatt gtgtaacaat
     4201 acatccatta ctcttcttcc aactttctgt taactactat ttctaagctt tttcttcttt
     4261 ctttggtgaa taaactgtta atccttttga tacaacagat aattcaactg gaagatcatc
     4321 tgtcgtgtct ccatccgttc gagtgcccac agatttttcc tgcgcttgga ttttcatgcg
     4381 tttggctgtg aaataagtaa tgccggggat cgtatagaat ttgcctctca tgattctagg
     4441 caaataaaaa ataaatcgga aaatatctaa ggaaggtacg atcgttacat gaaattttcc
     4501 atcatctagg gtcgctgagt cgtcaaaatt tgtaaaaccg cccacggagt tagacatcgt
     4561 gactgtcaac agttgggatt ttcctttcca atgctcctcg tctgtttcta aatccaaatg
     4621 atattttttc ttcttcagca ataacttaaa aaacttcttg ataaagatcc acgagccgta
     4681 acgctgtttt tcttcttgac tgatccaaac tgctgtatct gctaagattc cgattgtcag
     4741 tgtagagata atgacttcat cattgacttg accatagtct attttccgta cattgccttc
     4801 caggatcacg tcagcagctt cttctttttt gaggggaatc tcaagaacac gtgcaaggtt
     4861 gttgacagtt ccacctggaa gtaatccgac atggtattga gataatgttt tttggaacgc
     4921 ttggacgata tgatgaatcg tgccgtctcc acctataatt actaaagtat cagcttctat
     4981 ggcttctgct ttcttgacta attccgcatc atcaatcgat gggccagttt cagctagttc
     5041 tacatataat tctggctgct tttcttttac ataatcttga aaccagcgtg ctaatttctc
     5101 accctcatct ttccctgaac ttttgttata aaaaactagc agcttttcca ttctttacct
     5161 cctatttact cttcaaaaaa gagggcggga caaccaattc cattgtcctg ccctttagcc
     5221 ttagacagat gacattccat cattgggacg aacgccttgt cataagctgt tctttttcga
     5281 ttttaactct ttgtgttcaa ttctgcaatt tttacaatca cgtctgttgc tttttccatt
     5341 acttgtaaag aaacaaattc gaatcgtccg tgcatatttt ctcctccggc aaatagattt
     5401 ggcgtcggaa tacccatgta agagattttt gaaccatctg ttcctccacg aactggttcg
     5461 atcactggag agataccaag ctcgatcatt gcttgtttcg ctaattccac gatgctcata
     5521 tctttttcaa tgatttcttt catgttgtaa tattgatcat acatatctac tttgattcgt
     5581 tcttgatcaa atcgctgatt caactcttct tggatatgtg tgatttgtgc tttgcgtgct
     5641 tcgaattttt cacgatcatg atcgcgaatg atataactca tagaagcttc ttcaggattc
     5701 ccatccaatg ccatcaaatg gaagaaacct tcgtatcctt ccgttttttc tggaacttca
     5761 tcggctggaa gctgattatg aaaatcgatt gctaactgta gagcattgat cattgtatct
     5821 tttgccgtcc ctggatgtac atttttccct tggatcgtaa tatttgcttg agcagcacta
     5881 aacgtttcat actgcaactc acctaaagga ccaccatcga ctgtataagc aaaatctaca
     5941 tcgaattgct caacatcgaa tttatccgct ccgacaccga tttcttcatc aggaccaaaa
     6001 gccactttga tggtaccgtg agggatagaa ggattattga tcaagatctc cattgcagtc
     6061 atgatttccg caatccctga tttgtcatct gctcccagta atgtactgcc atctgttgtg
     6121 atcaatgtgt ggccttcata atttttcaaa tttggaaaat cttttgtatt caatgtatag
     6181 cgttcttccg catctagtac gatcgtagac tcgccatcat agttttcaat gatttgtgga
     6241 ttgactccta ctgcattaaa atctgctgta tccatgtggg aaataaatcc aatggaacgc
     6301 acttcatgat caacattgct tggtaatgtt cccataacga atccgttatc ttcattatag
     6361 aaaacatcag ctaagcctaa ctcttctaat tcttttttca attcttgcgc aaaagcaact
     6421 tgtgtctgag tcgatggagt cgttttgctt gttgcatcgg agcgtgtttc tgttttgaca
     6481 tatcgtaaaa aacgtggcaa aagattttcg tacatagtat tctctccttt taattaaatt
     6541 gaaatgggtt tgtatttgtt tcagaaacga aaaagttaat atcccaattt tcttcttgtt
     6601 tccaagattc aaatttctcg ataaatcgtt gcttgcataa cgattcaata taatgaccgg
     6661 gatcaattgc aatcaaccca gcgctttgca tatcatgagc cgtatgataa tagatatctc
     6721 ccgtgatata gacatctgct ctttgggcga ttgcttgcgg ataaaatttt tcaccgcttc
     6781 caccgcaaat cgcaatgcgc ttcacagagg attttgcatt ttttggctgg actattctca
     6841 gtccatctag ttggaacgct tcttttactt gttctacaaa tgcctctata gaaatttctt
     6901 gcggcaattc acctactcgt cctaacccaa acatttcgac tggttcatct atagcgaaaa
     6961 gatcataagc aggttcctca taaggatgag ctgaccgcat ggcttgaatc acttgttttt
     7021 cgatagtttc cggcaaaata acctcaactt ttgcctcttg aacttgttct gtcttcccta
     7081 ctttcccaat cgctggttgt gcaccagctt ccggagtaaa tcgaccgtgt ccaatcgagg
     7141 taaaacttgt accggtataa tcaccttgtg taccagcacc agctgcagct aatacttcac
     7201 gcattttctg tgcatgatcc acaggtacat aaacggccag ttttttataa tgaatctcat
     7261 gagtctttac taaataactc tccacttcaa taccgagaag ttcacaaaac cagtcattca
     7321 gtccatccca aataatatcc atgttcgtgt gtgctgcata gacggcaata tcatgtttca
     7381 acaaatctgc atacattttt tcttgtggct gatcagtaac cagccgtttt acaggacgaa
     7441 agattggagg atgtttagca atcagcaaat cgattttttt cttgattgct tcttcgacca
     7501 cttctggacg aacgtctaat gtcatcatta ctcgttggat tggcttatcc aatgttccga
     7561 tatgcagtcc gactgggtct ccttcttcag ctagccactg aggacaatag ctttcaaact
     7621 tttggatgaa agtgcgtccg ctgattgtca tggctttaaa acctcctcaa tcatttggat
     7681 ttcctgctcg atttgttttt gacgtgcctc ttgattagct gcttgtttga gctgttcacg
     7741 gatagctttt ctttgttgaa gctctcttgt ccattttgat ttgaagatct cacttttctc
     7801 ttcaagcaga aatggcccga acatcagctc catttcattg taagtaactg gtttttgttc
     7861 tttttcggct acaatgattt catatatttt tttattttct tcaagtattt tttctgattg
     7921 gattttatac tgattttctt gcagccaaga tcgtaatgta cgctctccga tgtttggctg
     7981 aaggatcaaa cgttcttgtc cgcttaagcg ttgattcaac ttgccagctt ctaggatatc
     8041 tcggatcagt gaacctccca tacccgcaat cgtgacagct gtaataccat cttgtggctg
     8101 gatagcatct agaccatttg caaggcgtac ggtgatacgg tcttctaatc catctttttt
     8161 gacttgctta cgtgctgatt cataaggacc ttgaacgact tcaccagcaa ctgcatattc
     8221 gatttttcct cttaatatca atgcaacggg taagtaagcg tgatctgatc caatgtctgc
     8281 taaacgagcg gattcaggga caaagtcacc cactgcggct aagcgctgag ataaatctaa
     8341 atggttcata gcttcactcc ttctagataa ttatagcatt ttccaaataa aataaggaga
     8401 ctaggaataa aaaa
//
LOCUS       DACDXE010000101         8159 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.101, whole genome shotgun sequence.
ACCESSION   DACDXE010000101 DACDXE010000000
VERSION     DACDXE010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.101"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..435
                     /locus_tag="IWM23_002610"
     CDS             <1..435
                     /locus_tag="IWM23_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAP9885203.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IWM23_002611"
     tRNA            complement(759..842)
                     /locus_tag="IWM23_002611"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IWM23_002612"
     CDS             1370..1870
                     /locus_tag="IWM23_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885204.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IWM23_002613"
     CDS             complement(2060..2404)
                     /locus_tag="IWM23_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAP9885205.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IWM23_002614"
     CDS             complement(2558..3478)
                     /locus_tag="IWM23_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAP9885206.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IWM23_002615"
     CDS             3665..4156
                     /locus_tag="IWM23_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAP9885207.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IWM23_002616"
     CDS             complement(4180..6555)
                     /locus_tag="IWM23_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAP9885208.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IWM23_002617"
     CDS             6881..7507
                     /locus_tag="IWM23_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAP9885209.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8009
                     /locus_tag="IWM23_002618"
                     /pseudo
     CDS             7530..8009
                     /locus_tag="IWM23_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
BASE COUNT         2532 a         1592 c         1424 g         2611 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgttgcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc aagacaaatg tttttcactt gtccacactg
     8041 gacaaaaatc ccatcaaatc aaggaaaagc aacgttggca atgtttgaat tttttaggct
     8101 agaacggact agtaagagtt ttttgcccct tttttgcccc tcaaatatcg ctttacaac
//
LOCUS       DACDXE010000102         8115 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.102, whole genome shotgun sequence.
ACCESSION   DACDXE010000102 DACDXE010000000
VERSION     DACDXE010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.102"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..197
                     /locus_tag="IWM23_002619"
     CDS             <1..197
                     /locus_tag="IWM23_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885210.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IWM23_002620"
     CDS             314..811
                     /locus_tag="IWM23_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9885211.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSIGELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMYDVARYYIEETGALGEVPASLQNYIDYQAYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            786..1292
                     /locus_tag="IWM23_002621"
     CDS             786..1292
                     /locus_tag="IWM23_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016897981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885212.1"
                     /translation="MGFLKSSIKSVGTLLLADFLFYGVAQSATPIFYERIDYMKKIRS
                     YTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAVMILGNLPPLSMIEGAFLKYF
                     GIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRPKLTYAGKKVTLGRNQPQEAITAVR
                     SEFYGISN"
     gene            1276..3723
                     /locus_tag="IWM23_002622"
     CDS             1276..3723
                     /locus_tag="IWM23_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885213.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSIRSAQERSKNEVTGKLKAVAYDKIDQQTDALISM
                     IGENQVNYRFFIGFKLLLNDQEFSMKSLTVEAKNALSDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYHLSKKKLDN
                     ETLIKYYDLIKPTRCLVEEKQRYLKIQQEDETVYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVTLTSDEKNKGLLDPYVIMKNPKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSNGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3726..5903
                     /locus_tag="IWM23_002623"
     CDS             3726..5903
                     /locus_tag="IWM23_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAP9885214.1"
                     /translation="MKPSIVNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVSANGFSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHAIMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNLTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRV
                     MRKPRMLMHAHMHRLQRKLGRSMTTLGAGSAIVTGKKGQSGSGSSARTQADHSRPDGK
                     EKSTLGKRIGQTIGTVADTKDRMVDTASGLKEQVKDLPTNARYAVYQGKSKVKENVRD
                     LTSSISQTKADRASGRKEQQEQRRKTIAKRRSEMEQVKQKKQPASSVHERPTTRQEQY
                     HDEQTSKQSNIQTSYKESQQAKQERPAVKSDFSSPKVERQGNTVQEKTVQKPATSTTT
                     ADRTSQRPITKERPSTVQRVPLQNTRSRPPIKTATIKKVGKKP"
     gene            5900..6901
                     /locus_tag="IWM23_002624"
     CDS             5900..6901
                     /locus_tag="IWM23_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAP9885215.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDSGISNIHYGGV
                     NVSAEVLAHKPMVEKYAKEYGVEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLSVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGGEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKYEGWRYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTQHIPLSEAQA
                     GDLVFFHSTYNAGSYITHVGIYLSNNRMFHAGDPIGYADLTSPYWQQHLVGAGRIKQ"
     gene            6898..7830
                     /locus_tag="IWM23_002625"
     CDS             6898..7830
                     /locus_tag="IWM23_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015646598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885216.1"
                     /translation="MRKEDLMMKFRKNQNKEKQIPKEKKPRVYKVNPHKKVVIALWVL
                     LGLSFSFAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWEQSDKSI
                     DNRMESLKGYLTDELQALNVDTVRKDIPVSSSVRGFQIWTVEPTGDNEFNVTYSVDQL
                     ITEGENTKTVHSAYIVSVYVDGSGNMVLVKNPTITNIPKKSSYKPKAIESEGTVDSIT
                     TNEINEFLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIHNRKDNQVTV
                     SLTVEYIDQQTKATQVSQFDLVLEKNGSNWKIIE"
BASE COUNT         2530 a         1547 c         1724 g         2314 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gactacgagt taccctttac
      481 ggttgacgaa tacacttcca ttggcgaact caatcgacta tgggaaatgg tatcggaatt
      541 acccgaagaa ttacaatcgg agctatctgc tctgctcact catttttcaa gcattgaaga
      601 actaagcgaa catcaagagg atattatcat tcattccgat tgtgatgata tgtatgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtaccagcta gtcttcaaaa
      721 ctatattgat tatcaagcct atggtcggga tttagacctt tcaggaacgt ttatctcaac
      781 caatcatggg atttttgaaa tcgtctatta aatctgtcgg tacattacta ctggcagatt
      841 ttctatttta cggggtggct caatcagcta cccctatttt ttatgaaagg attgattaca
      901 tgaagaaaat acgaagctat accagtatct ggtctgtgga aaaggtactg tattctatca
      961 atgattttag acttccgttt cccataacct ttacgcaaat gacatggttt gtcgtgtcac
     1021 tctttgcagt gatgatactt ggcaacttgc cccctctttc catgatagag ggagcatttc
     1081 tcaaatactt tgggattcct gtggctttca catggtttat gtctacaaaa acttttgatg
     1141 gtaaaaagcc ttatggattt ttgaagtctg tcattgctta tgcactgcga ccaaagctga
     1201 cctatgcagg aaaaaaagta acgcttggca gaaaccagcc acaagaagcc attacagcag
     1261 ttaggagtga attttatggc atatccaatt aaatacattg aaaacaatct cgtctggaat
     1321 aaagacgggg aatgttatgc ttactatgag cttgttcctt acaattactc atttctaagt
     1381 ccagaacaga aaatacaagt gcatgattct ttcagacagc ttatcgcaca aaatcgtgat
     1441 ggcaaaattc atgctttaca aatcagtaca gaatccagca tacgttctgc acaagagcgt
     1501 tccaaaaatg aagtcactgg caagctcaaa gcggttgcct atgacaaaat cgaccaacag
     1561 acagacgctt taatatccat gattggcgaa aatcaagtga actaccgttt ctttatcggc
     1621 tttaagttgc ttctcaacga tcaggagttt tctatgaaaa gtcttaccgt tgaagcaaaa
     1681 aatgctttgt ctgattttgt ctatgatgtg aaccataagc tgatgggcga ttttgttagt
     1741 atgagtaatg atgaaatcct gcgttttcag aagatggaaa agctcttaga aaataaaatc
     1801 tctcgtcgtt tcaaaatccg caggttagat aaggacgact tcggctatct gattgaacac
     1861 ctttacggac agacaggcac tgcctatgaa gagtatgagt accatctatc aaagaaaaag
     1921 ctggataatg aaacgctgat taaatactat gacttgatta agcctactcg ctgtttggtg
     1981 gaagaaaaac agcgatattt gaaaatccag caggaagatg aaaccgtcta tgtagcttac
     2041 tttaccatta acagcattgt cggagaactg gacttcccgt cctctgaaat cttctactac
     2101 cagcaacagc aatttacatt cccgattgat acgtcaatga atgtggaaat tgtagcgaat
     2161 cgtaaagccc tatctactgt ccgcaataaa aagaaagaac tgaaagactt ggataaccac
     2221 gcttggcaaa gtgataatga aaccagctcc aatgtggcgg aagctctgga aagtgtgaat
     2281 gagctggaaa ccaatttaga ccaaagcaag gaatctatgt acaagctgtc ttatgtggta
     2341 agggtatcag caaatgatct tgacgaactc aaacgtcgtt gtaatgaagt gaaagatttt
     2401 tatgacgatt taagcgtaaa actggtacga ccatttgggg atatgctcgg cttacatgaa
     2461 gaatttttac ctgccagcaa gcgttatatg aatgattata ttcaatacgt gacctctgat
     2521 ttcctcgctg gtttaggttt tggtgctact caaatgctgg gggaaaatga ggggatttat
     2581 gttggctaca gcttagatac tggacgcaat gtctatctga aacctgctct tgccagtcaa
     2641 ggggttaagg gttcagtaac caatgcgtta gcgtcggctt ttgttggttc gctgggtggt
     2701 ggtaaatcct ttgcgaataa ccttatcgtc tattatgcgg tgctttatgg ggcacaagca
     2761 gtgattgtag acccaaaagc agaacgtggc agatggaaag aaaccttgcc agagatttcc
     2821 catgaaatca atatcgtcac tctgacttct gatgagaaaa acaaaggctt acttgaccct
     2881 tatgtgatta tgaaaaatcc caaagattct gaatcactgg ctattgatat tctgacattc
     2941 cttacgggga tttcctctcg tgatggggaa cgcttcccaa tccttagaaa agccattcgt
     3001 gcagtaacca atagtgaagt acgagggttg atgaaagtga ttgaggaatt acgggttgag
     3061 aatacgccac taagtaccag tatagccgac catatcgaaa gttttacaga ctatgacttt
     3121 gcacatttat tattcagtaa tggttatgtg gagcagtcta tcagcttaga aaaacaactg
     3181 aacattatac aggttgcgga cttggtactt cccgacaagg aaacttcctt tgaggaatat
     3241 accactatgg agcttttatc cgttgctatg ctgattgtca ttagtacctt tgctttagac
     3301 tttatccata cagaccgaag cattttcaag attgtagatt tagacgaagc atggagcttt
     3361 ttacaggtag cacaaggaaa aacactatct atgaagctgg ttcgggctgg tcgtgctatg
     3421 aacgctgggg tatatttcgt gacccaaaat acagacgacc tcttagatga aaaactgaaa
     3481 aataacctcg gcttaaaatt tgcatttcgt tccactgacc ttaacgagat taaaaagacc
     3541 ttagcctttt ttggtgtaga cccagaggac gaaaacaatc agaagcgatt gcgtgatttg
     3601 gaaaacgggc aatgccttat cagtgattta tatggtcgtg tcggtgtgat acagttccac
     3661 cctgtatttg aagaactgct ccatgccttt gataccagac cacctgtgcg aaaagaggtg
     3721 taaatgtgaa accatcaata gtaaacagaa taaaatcaaa ctggacgctg aaacgtctag
     3781 gtaaagtggc aatgacagtg gctttcacac ttgtgattgc catttttctt ttagccatgc
     3841 tgggaacggt ggttcaagct gcgggcttgg tagatgatac ggtcaatgtg gcaaatgaat
     3901 acagccgata cccacttgaa aactatcaac tggattttta tgtggataat agctggggct
     3961 ggcttccgtg gaactggtcg gacgggattg gaaaacaggt catgtatgga ctatatgcca
     4021 ttaccaattt tatttggaca atcagtttgt atgtttccaa tgcgacaggt tacttagtac
     4081 aggaagccta ttccttagac ttcatttccg ctacagcaga ttccattggt aagaatatgc
     4141 agaccttagc tggtgtgagt gcaaacggat tttcaacaga gggtttctat gttggattcc
     4201 tcttactctt gattttggtt cttggggttt atgttgccta tacgggactg ataaagagag
     4261 aaaccacaaa ggcaattcat gccattatga attttgtgct ggtgtttatc ctatcggctt
     4321 cctttattgc ctacgctccc gactacatta aaaaaatcaa tgacttttca tcagacatca
     4381 gtaatgccag tttatcactt ggcacgaaga ttgtcatgcc ccattccgat agtcaaggca
     4441 aggacagcgt ggacttaatc agagatagcc tgttttccat acaggttcag caaccgtggc
     4501 tactgcttca atacaacagt tcagacattg aaagtatcgg tattgaccgt gtggaaagcc
     4561 tgctctccac cagcccagat tccaacaatg gcgaagacag agaaaaaatt gttgcggaag
     4621 aaattgaaga cagaagcaat accaatctaa ccattacaaa gaccattaac cgtttaggta
     4681 cagtcttctt cctatttgtc ttcaatattg ggatttccat atttgtattc ctattaacag
     4741 gaatcatgat tttctcgcag gtacttttta tcatctatgc tatgtttctg cctgtgagct
     4801 ttattttaag catgattcca tcatttgatg gtatgtcaaa acgagccata acaaagctct
     4861 ttaataccat tttgacacga gctggaatca cattgattat tacgacagca tttagtattt
     4921 caaccatgct ctatacctta tcggctggtt atccgttctt tttgattgct tttctacaga
     4981 ttgtgacctt tgcaggaatc tacttcaagc tgggcgattt aatgagtatg ttttctctac
     5041 agagtaacga ttctcaaagt gtgggaagtc gtgtgatgag aaaacctcgt atgcttatgc
     5101 acgctcacat gcaccgtcta cagcggaaac ttggacgttc catgactact ctaggggctg
     5161 ggtctgccat tgttacaggt aaaaaaggac agtcgggttc ggggagttct gcaaggacac
     5221 aagcagatca ctcccgacca gacggaaagg aaaaatcaac acttggaaaa cgtatcggtc
     5281 aaaccatcgg tacagtagct gataccaaag acagaatggt agacactgct agtggtttga
     5341 aagaacaggt taaagatttg ccgaccaatg caagatatgc agtatatcaa ggaaaatcca
     5401 aagtaaaaga gaatgtccgt gatttaacca gtagtatttc tcaaaccaaa gcggacagag
     5461 ccagtggacg caaggaacag caggaacaaa ggcgaaaaac cattgcgaag cgtcgctctg
     5521 aaatggaaca ggtcaaacag aaaaaacagc ctgcttcttc tgttcatgaa agaccgacta
     5581 caagacaaga acaatatcat gatgaacaga cctcaaaaca gtctaatatt cagacttcat
     5641 ataaggaatc tcaacaagcc aaacaagagc gtccagcagt taagtccgat ttttcaagtc
     5701 caaaagtgga acgccaaggc aataccgttc aagaaaaaac cgttcaaaag ccagcaactt
     5761 caaccactac agcagataga acttcacaac gtccaatcac aaaagaacgt ccgtctactg
     5821 ttcaaagagt accactacaa aatacaagaa gtagaccacc aatcaaaacc gccaccatta
     5881 agaaagtcgg taagaaacca tgaagttgaa aactttagtg attggtggtt ctggattatt
     5941 cttgatggtc ttctcactgc ttctgtttgt tgccatttta ttttcagatg aacaggacag
     6001 cggaatttcc aatattcatt atggaggtgt gaatgtttcc gcagaagtgc tggctcataa
     6061 gcctatggta gaaaaatatg ccaaagaata tggcgttgaa gaatatgtca acatacttct
     6121 tgcgattata caggtggaat cgggcggtac tgcggaagat gttatgcagt cctcggaatc
     6181 cctcggtctt ccacctaatt cattgagtac agaagaatcc attaagcaag gtgtgaagta
     6241 tttcagtgaa ttattagcca gtagcgaaag gctcagtgta gatttagaat cggttatcca
     6301 gtcctacaat tatggtggtg gtttcttagg gtatgtggct aatcgtggaa ataaatatac
     6361 ctttgaactg gctcaaagtt tctcaaaaga gtattcaggt ggcgaaaaag tgtcttaccc
     6421 caatcccata gccataccta tcaatggggg ctggcgatac aactatggca atatgtttta
     6481 tgtgcaactg gtaacgcagt atcttgtcac aacagagttt gatgatgata cggtacaagc
     6541 catcatggac gaagcactga aatatgaggg ctggcgatac gtttacggtg gagcttcccc
     6601 gactacttct tttgattgta gcggactgac acaatggacg tatggaaaag ctggaattaa
     6661 cttaccacga accgcacaac agcaatatga tgtgacccag catatcccac tatcggaagc
     6721 acaagctggc gatttggttt tctttcattc tacctataac gctggctctt atattactca
     6781 tgttgggata taccttagca ataaccgtat gtttcatgca ggcgacccaa tcggttatgc
     6841 cgacttaaca agcccctact ggcaacagca tttagtggga gcaggacgaa tcaaacaatg
     6901 agaaaggaag atttaatgat gaaatttaga aaaaatcaga ataaagaaaa acagatacca
     6961 aaggaaaaga aacctcgtgt ctataaggtc aatcctcata aaaaggttgt gattgccttg
     7021 tgggtacttt tagggcttag tttcagcttt gcgatattca agcactttac agctatagat
     7081 actcatacta ttcacgaaac aactatcata gaaaaggaat acgttgatac tcatcatgta
     7141 gaaaattttg tagagaactt tgcgaaagtc tactattcat gggagcaatc cgataagtcc
     7201 attgataatc gaatggaaag tctaaaaggc tatctgacag atgaacttca agctctcaat
     7261 gttgatacag tacgcaaaga tattcctgta tcgtcttctg taagaggatt tcagatatgg
     7321 acggtagagc caactggcga caatgagttt aatgtaacct acagtgtaga ccagctcatt
     7381 acagagggag aaaatacaaa gaccgtccac tctgcttata tagtgagtgt ctatgtagat
     7441 ggttctggaa atatggtact ggttaagaat ccgaccatta ccaacatacc taagaaatca
     7501 agttataaac caaaagccat tgaaagtgag gggacggttg attccattac aaccaatgaa
     7561 atcaatgagt ttttaacgac gttcttcaag ctctatccta cagcgacagc cagtgaactt
     7621 tcctactatg tgaatgacgg gatattaaaa ccaatcggaa aagagtacat ctttcaagaa
     7681 ctggtaaatc ctattcacaa tcgtaaggat aatcaagtca cggtatcgct gacagtggag
     7741 tatatcgacc agcagaccaa agcaacgcag gtatctcaat ttgatttggt acttgaaaag
     7801 aacgggagta attggaagat tatagaataa caaatattgg tacattatta cagctatttt
     7861 gtaatcacgt actctctttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt
     7921 gctattattt aagtatctat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa
     7981 taaactgtca atttgatagt gggaacaaat aattggatgt ccttttttag gagggcttag
     8041 ttttttgtac ccagtttaag aataccttta tcatgtgatt ctaaagtatc cggagaatat
     8101 ctgtatgctt tgtat
//
LOCUS       DACDXE010000103         8112 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.103, whole genome shotgun sequence.
ACCESSION   DACDXE010000103 DACDXE010000000
VERSION     DACDXE010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.103"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..110)
                     /locus_tag="IWM23_002626"
     CDS             complement(<1..110)
                     /locus_tag="IWM23_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885217.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELT"
     gene            complement(179..385)
                     /locus_tag="IWM23_002627"
     CDS             complement(179..385)
                     /locus_tag="IWM23_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885218.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
     gene            complement(548..2182)
                     /locus_tag="IWM23_002628"
     CDS             complement(548..2182)
                     /locus_tag="IWM23_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885219.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            complement(2186..3559)
                     /locus_tag="IWM23_002629"
     CDS             complement(2186..3559)
                     /locus_tag="IWM23_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885220.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            complement(3575..3814)
                     /locus_tag="IWM23_002630"
     CDS             complement(3575..3814)
                     /locus_tag="IWM23_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885221.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            complement(3996..6671)
                     /locus_tag="IWM23_002631"
     CDS             complement(3996..6671)
                     /locus_tag="IWM23_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HAP9885222.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            complement(6854..7777)
                     /locus_tag="IWM23_002632"
     CDS             complement(6854..7777)
                     /locus_tag="IWM23_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885223.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLKNINKTVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
BASE COUNT         2373 a         1595 c         1228 g         2916 t
ORIGIN      
        1 gttagttcat attctcgaat gatttctaca cgtggctttc cagctaagta aagattgacg
       61 atttgttgct tgaattcttg tgaaaaagtt cttcgtgttc tcttagacat aaaaattcct
      121 cctggtatgt tttcttctag tctacacacc ttaatttttc tgtctagtta attgtagcct
      181 atccagtata ataaacattt ccttgaggtt tccttttaat ccccttcgga aaatcacctt
      241 ttgtaaaatc gctcttaagg aaatcatcta aattaccagt tctggtcttt ttacctccag
      301 tattccctga ttttttaggt ggtacatgtt ctttagtaag ttcagtgtat ttttcacata
      361 aaatacactt acccatcttt ttcatatcta tccccactct ttttaattct tatatacaat
      421 tatatttcaa atattcatta ataaatatcc ctatttatga taaggacctc cctaactact
      481 tctctaacga tttcaaatcg ctagagaagc gttagcgccc ctctaacgtt gtttgatata
      541 acctagacta gatcgttaac gtaaagggtg tttttaacac aaaaatagcc gtatcctttg
      601 ttttggatac ggctatttga tattgctcat tcaattgttg taccacggca tgcgtgaatt
      661 tcatcgtaat ttccaacatc tccttatggt taggatcaaa ggttattttc tctataaagg
      721 tacgatacaa ggctttgatt tgcttcttct cactctgggc aagaaaacga tccaaattag
      781 tcaatatccg ttggacatct ttgacttgaa tcggctctcc ttgttgaaag atccctaaga
      841 tttcattttc tcgaatgtgc aactgtcgtc gtttttcagt caattcgtct aagcggtctt
      901 ttaacatcgg aaacaattca ggactatctt ccaaagcagc ttcccatttt tcaattttag
      961 tcttcaattc ttcctttcgt tccagtaaca ccacgagttc ttgttccaat ggttggcttt
     1021 gtcgcactat ttcttcattt aacgctgcca ccaatcgggg aagaacttct ggtagctgaa
     1081 cgatctcctt taatcggtcg gcgacaaatg tttccgcttg ttcggcacga atcgaattgg
     1141 catgacacac acttgctcct ttattccgaa agactgaaca agaataatat cgaatccgtt
     1201 tctttgtgcc atcctttagg gtatttgtga cattactcgc tgccatcggt gcgccacatt
     1261 ctggacaacg taacaagcct gtcaatacat tttcacctgt atggttccat ttgggttgtt
     1321 ttccttctaa agcaaggcgt tcttgtactt tttgataatc ttcttccgaa ataatcggtt
     1381 catgggttcc tttcactaaa atcggtctcg gattcttccc cttccgtcgt ttggtttccc
     1441 aattgatgta gcgcgcatat tctaaatacc ctgcataaat tttattgcgt aagatatctt
     1501 tgactgccgt ggtagaaaaa gcattctttt tgactgtttg atatccttga cgattcaaaa
     1561 aattggcaat cgctcgatac cctgctccgc ctaaatattt tgcatagatc agacgaacaa
     1621 ttttcgcctc ttctggttca atgaccaatt gtttttgacc attcgtgtca ataccactga
     1681 catagcctaa acgtcggcct cctgcataac gctttttatc tttcactaag ctttgcattg
     1741 acatttgaac attctccgca atttggttgc gttcaaattc accgaaactc ccaaggagtt
     1801 gtaacatcaa tttacctgtc gaagtggtca cgtcaaattg ttccgagatc gaatgtaatc
     1861 ctacgtcata tcgcaataat tcctcgactg tttgtaagac atctaacata ttgcgtccga
     1921 gacgggtcaa tttccaaatc actagtagat caaaacgatg gttccgagca tcgttcatca
     1981 ttcgttgata ggcgaaccga ttttttgtgg atttcccact gattccttca tcggcataaa
     2041 cttctaccac ttcatagccc atccgttcgg caaactgtcg tccattttgt tcttgtgctt
     2101 gaatactatg tccttctaaa gcttgttctt gtgtactcac gcgaatatac aatgcgactc
     2161 gtttggtttg tatcatttcc atagatcatt cgttcctttc taaaatattt tgtgtcagtt
     2221 gtcggaagta aatcccacaa actgttttat cctttgttac ctcaatccga caaacatagt
     2281 cacataataa ttgtcgacta gagaggtctt ttagttgaag ccattctttc acttttgctt
     2341 cacactgttg gagtgaaaga aacgaagaag tagacatcgg cttttgaaga gcttgcagtt
     2401 gttgcttgag ttcttccagt gaacaattgc cctcttcaaa tgccttaatc cattgtgctt
     2461 cttgtttctt ttgttggatt actttgcgtt tttcatcttt tcgcttttcg gctaattgtt
     2521 gcgttaattg ggcaatcaag gcttgataga cgccttcgta ttgaaaaaaa gcctgtatct
     2581 tttccaatac ttctgactca accctttctg cccgaagaaa aattccttgg cagcttcggt
     2641 cggtacaatg ataataatga agcgtcttcg tcttctttgt attccattga tacttcgaac
     2701 tcagatgacg accacagaag ggacaccgaa ttttttgacg taataagtac agatacgtat
     2761 cttttttagc tgtacacctt gattggcgaa tctcttgggc ttgtaaaaag gttgctttcg
     2821 agagaatcgg ttcaaaggtt cctagatact tccccaaaga tcctcctttg atttcgccat
     2881 agtacaatgg attttttagg atactataaa tatgataggg ctgaaaagga cgttcgcgga
     2941 agaaaagctt ttgttgcgtc aatcgttgac aaagcttttt gtagccatat tttttggtca
     3001 aataaagttg aaaaatatag cgaacaatcc ttgcttcgtg tacttcttgt tggattttcc
     3061 cattcactaa acgataccca tatggtaccg cagaagacaa atattgccgt tgttgctttt
     3121 tataggaaag tccggcacga acattctcaa taatgacgtt tcgttgttgt tctgcaaaag
     3181 tgaacaataa ttgcacaaat agtttattcg ttgacgcgtg tgagaaagaa gcaggaagag
     3241 gttcagaaat cgaatacact tgaacctgat gttgttggca caactcaata aattctaagg
     3301 ctattttggc ttgacgtgcc aatcgatcat aacgccaaac atagatcacc ccctcttctt
     3361 ctgacgcaca aacccaagct ctaagctgtc gaaaagctgg acgctccgct ttttcccctg
     3421 attctccttt gtctgaaaat cgttggggaa tttgttcata tttctctaaa acatactctt
     3481 tcactgcatc atattgagcg tcattactaa ttttcgcttc ttcataaaca gacgttcgat
     3541 cataacaagc cacacgcatg cttgattccc ctctctatga atgaactaaa taccattttt
     3601 tcacgactct tctattgaac gaacgttgca caaagtgatt ttctaaatat ccttcgttct
     3661 tcaacgcttg aagagtaccc ccgatagatc cagcagatac ccctcttatt tcgaaatcca
     3721 agtaaattgg ttttgtaaaa aaggcttgat ttgaaaaatg ttcttgaata tacagaagaa
     3781 cctctgcttt tagtgtcatt ttatttttag tcattagaat tttcctttct ttcatgtttg
     3841 attgaatgaa gtaaatgaag catagatcaa gcttcattta cttccgtttg ggtttgctcg
     3901 ctatcataca gataagtaat cagttgtttt gctaaatcaa taagaaatat ttccataaca
     3961 tggtgcctca ctttcatact ttggtttatt ctaaattaca atcgaaaata cgatttgagt
     4021 gaagaaggta atagaatctc gtatgttgga agaacttgac gattcatgtg aatacgtttc
     4081 gttcttctcc ctttttctgc acacatatta cccgaatcaa gcaattgcct aaaaagatag
     4141 gttagatcct ttacttgaaa ttctcgcctt aacaagtcct caaacgggat aggaaaaata
     4201 tttaccttaa tatccccatt tttttgaatt gatacttttc cccaaatcgt actgggcgta
     4261 tgtttgttgt cactgtttgt taaaaattta tggctattca tcaccaacaa tgttttcact
     4321 cggtcaaata ccgtttgctc acttcctaaa ctagtatcta caattaactg ttcgtacgaa
     4381 ttaataagca ccgtctgtat atgctctaat cgtaaaggat agcccagtat atcttgaatt
     4441 aggagggctc cactataaat caaagctaga ttattaccaa tgcgttgctt caaagaatgt
     4501 tcaggtaaaa cagcccatag ttttttttga gcttcgacaa aagttgcttc aatttttgta
     4561 atcccttgtt caaagagttt caaaagaaat aactttatac cccaaccaaa agtattagaa
     4621 gaaagacttt taactttctc tgcgtgattc gcatcctttg taaacttgat atcagaaaat
     4681 tcgatatatc gaactttcaa accagttaat ttctcattga aataattttc caaacgctct
     4741 tcagaagagg taagggtcaa aacagaaaaa gtctcttgtg gcataagctg acaattagaa
     4801 tctaatctta gcctttcttc tcctgaagct aattgataga tcaacgagct aaagttattt
     4861 tgagttgctg ctcctaactc atctagtatt agtggaacac ctccctgatt gtttgatgct
     4921 agctttataa tggctttatt ggtagcattc atatttctaa gaaccgtagt tggatttcca
     4981 aataaagagg caataaagtg taacgtagtg gttttacccg ttgtgctctt gccctgtagg
     5041 ttaattagta aactcaaaaa agtattagcc ttcgtaacgt ttaaatagcc taacacaata
     5101 gaagcaatac caatcgacca agcaagttct aagttcgttc ctaaaacata cttatgaacc
     5161 aatgcgtcaa attccgcttc tgtaccatta gttgttaatt ggtagcatga gtttttagtt
     5221 aatacagaag gaatttcacg tccaaaagaa tcttgtaact gaaaaatcat tgcttcattt
     5281 tcttttttcc agccaagaga ttcatgttca aaaaagattg gagcattctc ccgttgaatc
     5341 tgtaaaaagt caacgacttc agcttctctt ctagctttaa tgattaagcc atgttttgct
     5401 aattgtttaa aattattagt ttgtaaacat ccaccttcta tttctatttc atcccaacaa
     5461 tgatcgacat aataggcaag aacaagtgta atgacttttt gttcgatatt tttgcgtatt
     5521 tgcttaataa acatcggaca agcaactttt tcttcttgac cttctttcac caaaaatact
     5581 ccttcttgat tcagtttaaa ctggtcaaaa taatattctt gattggattc taatgattct
     5641 acagaaggat ttacaggttt tggcaacgga tattttttta caaagtcttg aatatcttta
     5701 tttttatccg aagcatgcag acaatgaaat cgaatcttat tatcagccgt aacagacaaa
     5761 caagaaccat tagaattatt cgtgtgtttt cttaatggac attcgtcaaa tataagtaag
     5821 gtaactcctt tatgatcgcc ctcttttatg gtataagtta attcaggata gtgagctaac
     5881 cattgtttat agtctgcttg aataaaaggt aacacttctc taaaagatgg atggcttttt
     5941 actctagagg ttgacatcat ggaattttct tcaactatct gttcaagaat tttaaaacaa
     6001 ttacttctat ctatgttatc gggaatagaa aggaaactag ataaacgatg cggacgctct
     6061 tttgtggact ttcctttgac cgctggtgta ccaactaatt tgcataatct tgaaggatta
     6121 taaacagaag tatcaatatc aacttcatct tctactccaa atcttcggtg aagtaattga
     6181 agaaaattct gaatagtaag aatcgtctcg tgattatttg gttgttcatt tatgggaaga
     6241 aaaatatggt agccattccc actatcaact tttacaaatg attctattct cttttctttc
     6301 aaataacgtt ctacttgttc cataacattt tctgcctttt tcttttcgct atctgttgca
     6361 cttccctctt tccttttagg gtcaatatca ataaatacaa accgaatttt ttcaatatca
     6421 ttgtctctca cagaatgctt tttttgtgta atcacctctt gtttaatagg attataagtt
     6481 atgtttactg taccttcaga caatttctgt ttttctaaat agttaataat tgcttgagaa
     6541 tctgcagtag aaaaaatatg agaaacggtt ttcttgttta atgtagaaaa agttctcaaa
     6601 ttaacataag aaatccccag tgtatttaac agttgtaaat gcttctgaat tgaagtgtga
     6661 ttcattttca ttaattacca ctcactttct aaaaaatttt tatttgtatg actacattac
     6721 ctccaacctc tcataatttc atgatcttca gttctacaaa aatatatccc actcaaaatc
     6781 ttgacaaaag aaaaaagttg tgattttgga aatcacaact tttttctttt acgataaatc
     6841 aatccttatt aaattattta ggaaaatatt gttcaattat ttctttatat attttctcat
     6901 agtatgtttc tgtccatcct aatgttccta taaaatcaag tcctctacat tttaaaggtt
     6961 cacttaaact attaatatag ttaataatgt tgtctatcct tttctcttta tcttcacctt
     7021 caatctgggg agcatatagt ccaatttcct cccaataata tgaatctctt tcaattccca
     7081 tgatggtagc tttggctaat ttcaaatcaa ataacgattt tagaattctt agtgtctcaa
     7141 taaagtcttc tctcttcaac tctttaatcc attgtgaatt ttctggatta tctaaaattt
     7201 caagttcatc ttcattataa gtatatttca aatttgactt gcttaactca tagataaatg
     7261 aagtttcttg actcataatt cttttaagta cttgactata agtttcgttt gactttgccc
     7321 atttcgctaa ttctaccact gtattcacta ataaatcgaa ataatgatgt tctcgttcaa
     7381 tagtctcagc attttgaaaa tgaaaatcac ctttataaac tccattcttt tccactgttt
     7441 tattaatatt ttttaaaaac cagaatgagt ttgcaaattt agtcgtactt tttcgattaa
     7501 ctaagttaat aactcttcca tgtaataata agacaaaaat acataaattt ttagcttcct
     7561 tattattaga tactaattga tcaatcatag ggagccattt tttgattttc actaattgat
     7621 tgctcataaa tctttcactg gacttttgtt caatcttaaa ttgcaaaact tctaactcat
     7681 aattcccatc ttctgattta tttttgggaa caactatcaa acttttataa agtttatcca
     7741 catcaaaatt tatctttttt gcataatttg tagccattaa tacatctcct tattcataat
     7801 aaatttcata tgacgctcct ctcaaaattt accggtagac tagaaaaaag aataaaagat
     7861 acgtcaattc acactttatt cttaaaaaaa aactggtctc tctaccggta atatcgattg
     7921 agtccgcctt tatcgacttt atcctttttt tatattaaat tttttcaccg gtagacaggg
     7981 agagaaagaa taaactaaaa agcccttaat tcttaatgtt ttcacctcct taccgttcct
     8041 accgctaaaa tttaatataa ctaaattcta taacattaga agaaatttta aatacctttg
     8101 ataaaaaata tt
//
LOCUS       DACDXE010000104         7622 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.105, whole genome shotgun sequence.
ACCESSION   DACDXE010000104 DACDXE010000000
VERSION     DACDXE010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.105"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(105..629)
                     /locus_tag="IWM23_002633"
     CDS             complement(105..629)
                     /locus_tag="IWM23_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885224.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWM23_002634"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IWM23_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAP9885225.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWM23_002635"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IWM23_002635"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAP9885226.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IWM23_002636"
     CDS             complement(2351..3031)
                     /locus_tag="IWM23_002636"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAP9885227.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IWM23_002637"
     CDS             3157..4026
                     /locus_tag="IWM23_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAP9885228.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IWM23_002638"
     CDS             4052..4567
                     /locus_tag="IWM23_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885229.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IWM23_002639"
     CDS             complement(4614..5177)
                     /locus_tag="IWM23_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885230.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IWM23_002640"
     CDS             complement(5232..5411)
                     /locus_tag="IWM23_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885231.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IWM23_002641"
     CDS             complement(5488..5997)
                     /locus_tag="IWM23_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885232.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IWM23_002642"
     CDS             complement(6093..6473)
                     /locus_tag="IWM23_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAP9885233.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IWM23_002643"
     CDS             6803..7552
                     /locus_tag="IWM23_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAP9885234.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
BASE COUNT         2168 a         1580 c         1354 g         2520 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aa
//
LOCUS       DACDXE010000105         7495 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.106, whole genome shotgun sequence.
ACCESSION   DACDXE010000105 DACDXE010000000
VERSION     DACDXE010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.106"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..126)
                     /locus_tag="IWM23_002644"
     CDS             complement(<1..126)
                     /locus_tag="IWM23_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAP9885235.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTF"
     gene            complement(139..1242)
                     /locus_tag="IWM23_002645"
     CDS             complement(139..1242)
                     /locus_tag="IWM23_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9885236.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1332..2267)
                     /locus_tag="IWM23_002646"
     CDS             complement(1332..2267)
                     /locus_tag="IWM23_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAP9885237.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2569..4503
                     /locus_tag="IWM23_002647"
     CDS             2569..4503
                     /locus_tag="IWM23_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAP9885238.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4960..5376
                     /locus_tag="IWM23_002648"
     CDS             4960..5376
                     /locus_tag="IWM23_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAP9885239.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5390..5956
                     /locus_tag="IWM23_002649"
     CDS             5390..5956
                     /locus_tag="IWM23_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAP9885240.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5979..6965
                     /locus_tag="IWM23_002650"
     CDS             5979..6965
                     /locus_tag="IWM23_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAP9885241.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7120..>7495)
                     /locus_tag="IWM23_002651"
     CDS             complement(7120..>7495)
                     /locus_tag="IWM23_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9885242.1"
                     /translation="TTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDST
                     DTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITL
                     AGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2151 a         1437 c         1542 g         2365 t
ORIGIN      
        1 aaaagtgttt agccaatgta tctcatgaaa aaaagcgata gagaataata cccctaccgc
       61 aaaaggaaat acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag
      121 tgtcatggtt cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc
      181 ctccgataat ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt
      241 cacgaagatc gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt
      301 cacacccatt gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat
      361 gatcgtgaca cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg
      421 atggtatttt ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt
      481 tttgttccgt tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat
      541 gccgactgtc cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc
      601 gcccaagtca acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc
      661 cgtaatgtaa cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata
      721 aacagttttt tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg
      781 atcgatttct tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt
      841 gattgcacca atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg
      901 cttgactaac gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt
      961 cacttcatcg atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac
     1021 tcgatcgaag gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac
     1081 gtaagtccct aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc
     1141 tgaactttca gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga
     1201 gatgcccagt gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt
     1261 ttgaaaggtg agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta
     1321 tgattatgtg attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct
     1381 ttttcagctg cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta
     1441 gcacctgtaa caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg
     1501 aattcgtcgt tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata
     1561 ttttcattat attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag
     1621 tttgattcag ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta
     1681 gagtagttct tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt
     1741 tcgattgtat ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg
     1801 aagctttcag atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct
     1861 tttaagaatt ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct
     1921 tccatgtttt tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta
     1981 tcatattttg attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa
     2041 ccatttgaat aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct
     2101 acaacttttg cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat
     2161 tcagaagaag agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct
     2221 gaaaatgcta atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac
     2281 cattccattt ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag
     2341 cctataagtc aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag
     2401 caaatttcgt gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac
     2461 aaaagaacag aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga
     2521 attatcatat attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa
     2581 aatattgtcg ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag
     2641 aaactgaaaa aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg
     2701 atgaccgagt tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat
     2761 ctgcagcatt tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt
     2821 atcgataaag aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg
     2881 atccttggaa tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga
     2941 ttcacccttt ggtcatttga agattctgta aggattcggg aacatattga gaagacagta
     3001 gcaaaagctg cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc
     3061 tgtggttctg gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc
     3121 ctagccaaag attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg
     3181 ccgacgatct tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt
     3241 aagcacaacg tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc
     3301 aaattgaaag acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa
     3361 ggaacttctg acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc
     3421 aatgaatata tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta
     3481 gtcttttatg aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa
     3541 acaggacaca cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa
     3601 tttaaaggaa actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac
     3661 ctatttgata agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat
     3721 ttgaaatact tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag
     3781 tttttccata tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat
     3841 gtcttatgga gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc
     3901 aaaaaaatca ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca
     3961 tggttcactg gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc
     4021 agtggtgctt ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc
     4081 ggagaagcag cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca
     4141 atggctgtga ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca
     4201 aacaatgatg tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca
     4261 ttagcattga ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg
     4321 atcacgttct gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct
     4381 ttcgcattga tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg
     4441 ctacaacgta cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa
     4501 taaatagata aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac
     4561 cgtcacagcc tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat
     4621 gagcaagaca caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga
     4681 agtagcttct tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa
     4741 attccttctt atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt
     4801 tccacctgct gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc
     4861 tattcttcgt tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc
     4921 taaaattggg tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct
     4981 gaaaaaagtc tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct
     5041 tttttaccag tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg
     5101 gtactgcgtg tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc
     5161 aacacatata cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat
     5221 cgagtacgga taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca
     5281 acgaaaaatg gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca
     5341 acagatggag gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt
     5401 acaaaaaatg atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga
     5461 aaatatgatc ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct
     5521 aatcacgatc gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct
     5581 acgactattc ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag
     5641 tggtgctttg cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt
     5701 cagtatcatc ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat
     5761 ggcttctttc attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt
     5821 agggatttta tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca
     5881 gacacttcaa cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat
     5941 gtttaccaaa aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg
     6001 aaaactatcc tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg
     6061 tccgcctgaa aaacaaacct gtcgaacaag ccatcaaaga aacgatccat gccggcggaa
     6121 agctgttgcg tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag
     6181 acaaagccat cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg
     6241 atgatatcgt tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg
     6301 gtaatgtcgt ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt
     6361 ctgattactc cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat
     6421 tgaatggcga attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat
     6481 atttggaaaa tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg
     6541 cttttgaaag tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta
     6601 tcgggatggc ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa
     6661 tcggcaaacc tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact
     6721 ctttgcaaaa aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag
     6781 aagaagatgt cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta
     6841 ggaagttagc ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca
     6901 cctctcttcg aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag
     6961 actgaatagg gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt
     7021 ttgacaaatt gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt
     7081 ccgttagcga cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata
     7141 actcataaaa gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca
     7201 attattcgat tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt
     7261 gcttgtttta ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga
     7321 gacacttgca tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt
     7381 tgatgaatct gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga
     7441 cgtgtccgtg ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggta
//
LOCUS       DACDXE010000106         7408 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.107, whole genome shotgun sequence.
ACCESSION   DACDXE010000106 DACDXE010000000
VERSION     DACDXE010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.107"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(13..348)
                     /locus_tag="IWM23_002652"
     CDS             complement(13..348)
                     /locus_tag="IWM23_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9885243.1"
                     /translation="MKMNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSK
                     YHTKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQ
                     RLEERMEEL"
     gene            complement(332..604)
                     /locus_tag="IWM23_002653"
     CDS             complement(332..604)
                     /locus_tag="IWM23_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HAP9885244.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(1055..1858)
                     /locus_tag="IWM23_002654"
     CDS             complement(1055..1858)
                     /locus_tag="IWM23_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAP9885245.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(1836..2960)
                     /locus_tag="IWM23_002655"
     CDS             complement(1836..2960)
                     /locus_tag="IWM23_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9885246.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(2976..3791)
                     /locus_tag="IWM23_002656"
     CDS             complement(2976..3791)
                     /locus_tag="IWM23_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAP9885247.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(3781..4662)
                     /locus_tag="IWM23_002657"
     CDS             complement(3781..4662)
                     /locus_tag="IWM23_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAP9885248.1"
                     /translation="MSEMKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAP
                     TKEFVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNL
                     YKPLFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAG
                     ITAWKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVM
                     TIVQGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAV
                     FLGVIFKFVEKGVYYEN"
     gene            complement(4738..6027)
                     /locus_tag="IWM23_002658"
     CDS             complement(4738..6027)
                     /locus_tag="IWM23_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002581297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAP9885249.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(6255..6899)
                     /locus_tag="IWM23_002659"
                     /pseudo
     CDS             complement(6255..6899)
                     /locus_tag="IWM23_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2278 a         1589 c         1136 g         2405 t
ORIGIN      
        1 tttttagtat tattataatt cttccatgcg ttcttccagt cgctgtaaat cgcttggact
       61 taattcccca atttttttcc atttgacctt tttgttgttt ttgggtaatt cgtttagagt
      121 tcctacgtca atccaagatt ctttttttag tcctgcctgt ttccaatccc ttatttcaac
      181 gtatttcagc tttattttat cggatttagt atgatatttt gaagtaattg acagaaacga
      241 aattgtgtcc agtccatttt ttagaatata gacaggccgg cgtttaccgc cctctgcagc
      301 tgtgaaagca acgtagacgg ttgctatatc attagtattc atcttcattg aaccattctt
      361 caattttttc gtcagtgtct agtttttgga caggcaattt ttccgctaat ctttgaatat
      421 tatggattcg tttacttttt tccgacaatt caatagaaaa tgggattttt ccttgctcaa
      481 caatttgctt ataaaaaacg gtgacggctg ttttggggct catgcctagt tcatcaataa
      541 tgctttctgc ttcatttttc aatttttcgt ccatagaaat ttgtaactta gccattttct
      601 ccacctcttt ctgacaaaca cattatatca cggaaagagt tcaaatggaa ttaatacaac
      661 tacttttgaa ctccttgaaa tgcagtgtat ttatctaaat ataaggaaag gatatagtgc
      721 taaatggcct tgatagatag aaaagtttca aaatccgcca cagcgataat tgaagcgaac
      781 actcaacgac acacagaaca ggaaaagact cctaaaagaa ccaaattaac taggacgtgt
      841 ctgaaaactc cacaaagatg tggtatgctc tggtaaaaaa cgaatggcac aaccacgaag
      901 accgatatgg ttcttggaaa actttctcca ccctttttgt aattggaggg atagtggttt
      961 attgatagcg attttccaag ttcttcaagt ggaaccagac attgagcacc acggctaaaa
     1021 agtgaatgaa gtctttattt ttaatcaata atttttaatg attaccagta attggttttt
     1081 tcatgcgccc tatttctatt tgatgtggag ttaaataatg ctgataagta aaaatttttc
     1141 cgtcaattaa actgccacta gtgaatcctg ttctattggt aaaaagagaa tagtctgggt
     1201 attcatctac accaaatgat aaagattcag ccataaactg ttttttacca gcaaattcag
     1261 cactgctacc ttgatggata tgtcctacaa aaatacctaa aatatcactt tcggcaatca
     1321 attcagcaaa accttccgga acttcagtta caatagatgg atcttcccac gacaatggat
     1381 gatgaaccaa taaaatcgtt cccttttcag caggtgtacc caaccactct tgtaattgaa
     1441 tcatctgtcc tatcgatatt tttccttcgt gctgggtatc ttgagcagaa tccaaggaaa
     1501 taattcgtaa cccattttga ttcgtacaat ggatatactc tcccgtttca ttgctttcaa
     1561 cactcattcc ttcaaagaaa acactgcgac gatcatgatt cccacgacaa aaatagtaag
     1621 gtgtggttgg gaaatattga tccaacattt tccgtaagac ttgataatca gaaatccccc
     1681 cttcatgtac taaatcacct gttaatagaa taaaatcgta cactgttaac gagagttctt
     1741 gtaacaattt ttccaatgcc aactgtggat ttttaattgt tggaaatagt tcagcagtaa
     1801 acaagttagt ttgatagttt tctctaaaat ggatatcaga gagatgcaaa aagcgcatgg
     1861 ctattccctc tttcttcaat aaaataatca ggatatccta aattttcttg cgtctgataa
     1921 tcaaaaaaat gcattctttc aggttctatg cggagattga ctgcatcacc aatctgccaa
     1981 gtaggttttt cattaaggac aatcatttct ataccatcga ctttaacggt gtacgcataa
     2041 ctttgaccta atagctcacg atacttcact tgaccgtgta aatcgcttgt ttcttttgaa
     2101 aaggttacgt gttctggtct tatgcctaaa tacaatgagc cactaacctg aagaagttgt
     2161 ttttgccaag ctggattcaa tgttaaaaat tgactggcta cctttaatgt tccttgatca
     2221 taagtaactg gcataatatt catgcctggc gaaccaataa aatttgcagt aaaagtattt
     2281 ttaggtttcg tataaacatt tttcggcgta tcaatcatct gaattctacc ttcattcaat
     2341 aaaacaatcc ggtcagctaa cgtcatcgct tcaatttgat catgagtgac gtagacaaaa
     2401 gtttgtttat aacgttgatg aatttttacc aattcttttc gtgcatccaa tcgtaaacgc
     2461 acatccaaat ttgataacgg ttcatctaat aaaaaataat tactccgttt aaccaccgca
     2521 cgagccaatg ctacacgctg ccgttgccct cctgacaaat ctttaggaag tcgatcttgg
     2581 tatggcgtta aacctaacat cttcaaagct tcttccaaac ggtgctgaat gtcagctgtc
     2641 ttaattttat tcgcccttaa tgcaaaaata atattctcct ttacagtcat gtgaggatat
     2701 aaagcataat tttgaaaaac cattgcgatc tctcgttctc cacaaggtag cttatttgct
     2761 agttgtgtac ctaaaaaaag ctctccactc gtaatttttt ccaaaccagc aatcattctc
     2821 agcaaagtag attttcctga accagatggt cccagtaaca caattcgttc tccagcggcc
     2881 acagctaaat caatttcttt taaaatggta tgttctccat atttttttcc gatatttctt
     2941 aattcaagcg tttctcccat gatttgtcct ccaatttatt taatacctgc agaaacaaaa
     3001 gtcccgataa tatatttttg aaaaattgca tacaagacta acggtacaaa cattgtaatt
     3061 aaactaatcg ccattgctac ggtaaaattt gtcccacctt cagaactaat ataggtctgc
     3121 atcgccaacg gcaaagtata attatctact gtccgtaaaa ttaataccgg ccaaacatac
     3181 tcattccaag aggtaataaa aaaccaaata ccggtagaag taatcgcatg tctagtaaga
     3241 ggaaaaacaa ttttactcat gacggttgtt tgtttgatgt tatccaatgc cgccacatct
     3301 agaaggctca ctggaatttc ccgcattgtt tgcgtcaata aaaaaatgcc catcgcatca
     3361 gcaaattgtg gtattatcac accccaagta gtatcaatta aattaagttt tgcaatcaac
     3421 aaataattag gaatcatcgc aacagtaaaa ggaatgaaca tcgtaccaat gatgataaag
     3481 taccagattt tcttcccttt gattcggcaa taagtaaaac tatatgccgc aaaaaaagct
     3541 acgcctaact taacaaccgt tataattgta gcaataaaaa aagtatttct cgtaattttg
     3601 ataattggaa gattttcgaa caaatagcaa aaattttcca aggtaaacgg ctgtggtatc
     3661 aaactagtaa tcgtctgata ggcatctttc aatggcttaa aagaattggc cataataaac
     3721 aataatggat agataataat gattatgatg aacataaata agagatgtgg caaccagcgt
     3781 ttaattttca tagtaaaccc ccttttccac aaatttaaat attaccccta agaaaacagc
     3841 aaatattaat aaagttaaaa ctgaaattgc tgaagattct ccgactttat acatttcaaa
     3901 agccttttga taagcataat agatcacgtt tgcactacca ttattcggtc caccacgagt
     3961 gatgatctta atcggagtaa acacatactg cagaccttga acaatggtca tcaccaataa
     4021 atatagtcct gtcgaactac tcataggcaa cacaattgta aaaaaaatgc gccagatggg
     4081 tacacgatcc aacttagctg cttcgatcat ctcttgatca attcccacaa ttgctgcaaa
     4141 aatgacaatg aaattataac caaaaacttt ccaagcagta atacctgcaa tcacagcaat
     4201 tacccaacct tgtaaattca tccacatagg cagtcttaaa ccaaaatttc ccaacaagac
     4261 agcaattggt cccgataccg gatttaatat ccatgtaaaa accatccccg ccactaccaa
     4321 agaaatcaca gctggaataa aaaataatgg tttatacaaa ttctgccctt tttctataac
     4381 tattcctaat aaaaaggatg taatatatgg gaacacacag ctcagcaaga caaatagaac
     4441 ggtataggac aaagtattct tcaacacttt atgaaagact ggatcagtca gcacctttac
     4501 atagttggca aagccaacaa attctttagt gggggccacc atattccagt caaaaaaact
     4561 caaatagatc gtataaatca atggataaaa aacaaacacg cccaacaaaa taattgctgg
     4621 cgccagatac aaataaccta catagcgctt catttccgac agcctcctca tccttatgtc
     4681 aaaagcagac gaccttgttt ttcctttctt acaggcgctg tcgtctgctt gctttgctta
     4741 tttgagtaac tcattaattt ccttttgagt agctttcatc gtttcattga cggaattacc
     4801 tgataaaatt ttatcccgca tatccaacaa tttttgttca gcttctaatc cagcatctcc
     4861 aggaaatgaa ctccaactga ccatcttatc catctgatca attgctggta tcatcatctg
     4921 attttcagct aagaaagctt ttaatccatt ttgggcgtct gcaacatctt tacgtggtgg
     4981 taaataacca gtaccttctg tccatttagc taaagattca acactataaa gatattttaa
     5041 gaaatcccaa gttgcttcct ttttcttatc cgtttgtgat gtaacagcta acattgcgcc
     5101 accagcaggt agtttaactt ttttcccttc ccaacttggt gactcaactg ctttagcagt
     5161 aaaagtacta tttttttgaa tgttgtccct ttgagcaata gtggtataga ccatcgcgac
     5221 gttaccatca ataaagcttt gcacaccttg ttcccacgta gtgtgtaatg ccgtctggtc
     5281 ttttaccatc ctttgatata attcataagc agcgatacca tctttagaag caaaggtagc
     5341 tttgccatct tttattacac ttgccccatt actttctaaa attgcttgtt gtgcccaatt
     5401 atcagcaggt tcttgaatat acacgccata ctttccagtt ttttctttta ttatcttaga
     5461 aaaagtttga acttcttccc aagtttttgg accattttca tccaagcccg ctgcttttaa
     5521 tatatcttga tttaaatata aaaccggatt actaatggag taaggaatac ccacttgttt
     5581 accatctttt tcagacagtg ccatgatatt gtctaaaaaa tgatctttaa aataatcagc
     5641 attatccgga tccacttctt ctactaaaga ttgaggatta gtatatttga aattttctgc
     5701 aaaataatct ttaaaagccc atcccacttg tactacgtcc ggtatttctt tggaggcaac
     5761 agaagcttgt aaattttgca tcaagccttt atacatatca ggattatatt ttgctacaac
     5821 cttcactttg tcttgagatt gattgtattt atctactaat tcattcactg tttttccacc
     5881 tagggtatcc gcattaacat gccaatactc tatcgttaca cgaccctccg atttttttgt
     5941 tgtcttggta ttcccactac cacaagctgc caaagagatg acacttaaaa cagacacaac
     6001 ccctataaca agatttttct ttaacataac attaacttcc tcctaatttc tttatggcca
     6061 tttttctaat caagaaatat tatgaactac caatgtaaat ttctagttaa ttttctgtaa
     6121 ataattcgaa atttgtgcat ttcggggtac agggtcactg gacatatcca tcaagctaag
     6181 aataaagccc ccatatggtc atgtatccca tctccaaatc tcttaactcc tctgttttga
     6241 ttataaaata gtttattctg tgctcctttc ttccataaat aggcccatgc acaacgtgag
     6301 gttatattat aaacaccaca gcgcgcaagg tactcaatcg tttctagttc attgactgtc
     6361 catacagaaa atgtcaatcc tagctgctgc cccacttcac gggccaactt agttaaccat
     6421 ccttcatgaa tattgacgat ttgaacgctt tgataggcta atttctgtaa ggtatcgatc
     6481 aaatctgctt cggtaatatc cccaagtttt ttcccttcga actgattttc aatattataa
     6541 aatatatgct ttccccaaat cgcagcccaa gataattgta catagccact aagaacaatt
     6601 tgtggccaga tttgataggt ttgtgccagc gaaaaaacgg ccatttccaa tccctcttct
     6661 tttaaatcac agttaatggt catggttgcc ttctcattgg ccactgcttg aaacacctgc
     6721 tcaagggtta ccgaatctgg gacaacttct tacctgtcat gggctagtat tagcgtacca
     6781 tctgtttgct gacgcacatc tacttcaaaa caaagctggc gatccaaaca aaagcgaaga
     6841 aatgcccagc tattctcctc cgtattgtca ctaccagagt gtgcagtgat catggtcatt
     6901 ttgacccctc ctttaattcc gtgattttct cccacacgtc cactataccg atgaagcgtt
     6961 aatttcttgt taatttcttg taaattccct gcgttttttc agtaaactag ttgtaaatct
     7021 tgcatgctcc ttcctacgaa ctcatgaatc gtcgattttg cccttctagc tagtttcacc
     7081 aattaagtag ttctacattc cccgctttta acctactaca cctttttttg ttttcagcta
     7141 ttccttcttc ttgctgaaag tccttgaggt tctttcttac aggataccat caaggtgttc
     7201 aatggcttat tcccattgaa tgaccgagac ttactatcag atttctgctc gttgaaccaa
     7261 gtctttgata caggatttct tccgtcatct tctcacaaat accaccttgc gtttctctag
     7321 cgattggtcg atgtgcaccc tcccagtgga ctgtcactac ccctttttgc tatgcctagc
     7381 ccacaaaaaa agctgtcctc acagacag
//
LOCUS       DACDXE010000107         7151 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.108, whole genome shotgun sequence.
ACCESSION   DACDXE010000107 DACDXE010000000
VERSION     DACDXE010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.108"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(38..3187)
                     /locus_tag="IWM23_002660"
     CDS             complement(38..3187)
                     /locus_tag="IWM23_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HAP9885250.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSPEQINRMIDQMESVKKESYLDPTVFEAEEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLVIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PEKTKGLIVTFRKPATMEKNVEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEE
                     QIGVYETVKGSLMEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHA
                     FSNEWFVSEKELHASNLQYMPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(3201..4418)
                     /locus_tag="IWM23_002661"
     CDS             complement(3201..4418)
                     /locus_tag="IWM23_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAP9885251.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKMFV"
     gene            complement(4408..6003)
                     /locus_tag="IWM23_002662"
     CDS             complement(4408..6003)
                     /locus_tag="IWM23_002662"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAP9885252.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            6128..6913
                     /locus_tag="IWM23_002663"
     CDS             6128..6913
                     /locus_tag="IWM23_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HAP9885253.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT         2080 a         1452 c         1183 g         2436 t
ORIGIN      
        1 tatctttgaa tcgtacaatt ttcttaactc ttctcgttta cctcaattca ttttcaagtg
       61 gaatcaactc atcatctaac attttacgcc atgctcgctt catttcttgt gggtatttaa
      121 atggtttcgc ttctggatgt ttggctttat atccttcgta atccttactg ttgataattg
      181 ctttcatatt aggaattgga tcttcacctg gcatatattg cagattagaa gcatgtaatt
      241 ctttctcaga aacaaaccac tcattggaaa aggcatgaat caaattatca cttgcttgtg
      301 taaaatagcg acgtttaatg acaaagatat cttcattact atctaaagtc cccacatcaa
      361 atgcatttaa aatttgtcgg taaacttctt tgactccttc tgttttattc aatttagcca
      421 aagctttttc aatttcttct cgtgcatctg aactattcgc agcataagtt cctagtaatt
      481 gatcaatata agtagaatca atatcataca acttagtggt attttcacta taaaattcga
      541 tcgttgaaaa atcttctccc tctttttctg gttcttcctc catcaatgaa cccttcacgg
      601 tctcgtacac gccaatttgt tcttccaaaa gttgaatttg attgttcaaa gttgaagatt
      661 gcaccatgtc atcattgtaa tcatcataag tcactaacga ttcatacgca cgattcaatt
      721 cttgaaatgc tttgacgtac tcgatacgga tttcaagtgg cgtatgttca tcaatggatt
      781 ccatggatga aacctctttt aacttaccat atgcttgctt gaatcttttt ttggattctt
      841 ggtaacttgg ataaatcaaa cccgattctt ctttagcttc tgaatacagt tttgtcgcat
      901 cctcgacatt cttctccatt gtcgctggct ttcggaaagt aacaattagt ccttttgttt
      961 tttcaggata cgtacggttc gtacgtgaaa aggcttggat caatcctgca tactctagat
     1021 tacgatccac aaatagggtt tgaatcgtag gggcgtcaaa acctgtcaat agacgatcca
     1081 ctacgattac taaatcgatt tgtttcccaa attgtttgaa ttccgctctt tttcgtgcca
     1141 aacgattatt gatgtctccg ttataacgct caatgtctgc tagtgaccag gcagtatcat
     1201 aatactgatt atattcctca ataatttttt gcatttcttc ttgttgggca cttgaatctc
     1261 ggctattttc ttctaatgaa taggtaatag cgattcgtgg gaaatcagga tcctccatcg
     1321 ttcgtccttc acggataggc tgattgggaa actcttttat taaccaatca gattctttcg
     1381 tcatctcttt gatggcccga taatactttt tggccatatc aatggaactc gtggttaaga
     1441 tcgcagactt cgttggacgg ccatttcgaa aatcaaattt tgtataggca ttgtctggac
     1501 ggaacatttt atgaatgact ttttggatat gttcctctgc ttcaaaaaca gtagggtcca
     1561 aataactttc ttttttgaca gactccattt gatcgatcat acgattgatc tgttccggtg
     1621 aatactctgc atatgtttct acttctttta atttccgata gattttattc tctaatgaag
     1681 tcggctccac ggtgttttca tgctctactt gaaatcctaa aaccgagcca tcttctaatg
     1741 cattcttgat ggtataggtg tgcaagacct ctccatactg atcatgggtc gtacgtgcca
     1801 attgcccttt ggcttgtttc cgattctcag ggaaaatcgg tgtacctgta aagccaaacc
     1861 aagtggattt cggaaacatt tttttgattt cttccatatt ttcagcactt aacgctcgat
     1921 gacactcatc aactacaaac acgatatgtt gccccattaa tttttggaag cgattggttc
     1981 cttttttctc ttcctgtttc tttgcatatt tcaacgccgc atctagtttt tgacgggttg
     2041 taatgatcac aacattcgca ttggcatctg ccaacagagt ttcacttaac tctttggcac
     2101 ttcctgttcc cacgatcaag ctattcgctt tggcattacc agaagaaacc ccagtattaa
     2161 actccgaagc aaatttcgta aactctgtgg tggtctgatt atctaaatct ttgcggtcca
     2221 ctaacatgat cgttcgatcg atccccgatt ttcgtgctaa aagcttagta gatacaaaac
     2281 tagtcaacgt ctttcctgaa ccagtcgcat gccacacata ccctgattga tgtttatttg
     2341 cggcaataaa caaggcttga atcgcatgca cttgataagg atgtaacacc atgagggttt
     2401 tattgtcttg atcttcactg acaatcgtat aatccgcaat caagcgatgg gcatctggga
     2461 tatttagcac ttgtttgcaa aactcatata agttttctac ctttttatta tctttcgttc
     2521 gccaactaaa taaaaactta ggatgcaaat ccttcggtaa ggcatttgca aaataccgag
     2581 tcgtttgttc attggaaacc acaaacaact gtaaggtcga aaagatattg ttccgaaaca
     2641 tgccttcttc agcatatttt ttgatttgat taaacgcctg ataaaaacca tcttttgcag
     2701 ttacttgttt tagctcgatt tggataatcg gtaagccatt gatcaataag gtgacgtcaa
     2761 aacgtcgatc ccgcgcttct atgttacttc cacgttttgc aatttgatgg accacttcat
     2821 aagtagagat tcctccacca atgtcttgat tggaataaag gataagtgag acagaaccca
     2881 attgcggatt ttcccgttca atcgtgatcc gagccatccc attttcacct tttaaccatt
     2941 tcgcagcatc aaatggggtt ttcgtccgta gcaataattc ggttttgatc gtttcaaact
     3001 ctttatcggt caaaggagaa tcattcagtt ctgcttgatt atttgagatg attttttgac
     3061 gtaaattgct ccaaagatct tcttctgatt ttagatccgg acgatacgtc cactgattat
     3121 gtccttcccc gaggacacga atcaactgtt cttcgacttc cgcttcctca cggtgactaa
     3181 ttttcattct tcttccctcc ctaaacaaac atcttttgca agaagccttt tttcgtttcc
     3241 ttcagcaaat ctaacttacg ttgatgaaga gcgatcgtat catctagctg cttgaagaag
     3301 gcaccaattt tttgttgttc atttatatcc ggtggaaact gaaatgtcct ttgttctata
     3361 ccacctttag tcacatgtac cattgtcgtt ccgttagttg attctttaat tttctcttta
     3421 tcagtcaata gccaagtata tagatactgt ttgtccaatt ccttagataa aaatttaagt
     3481 ttccaaatat gataatggta gattacacgt tcttcattcc acatttcagg accgaagctt
     3541 gttgcccata aatataagag atctccttta ttagcatact tgtcttcctc taattctaag
     3601 tttgaataat accatctatc attagtatta aaattaccta ctcttaaaac cctatatttt
     3661 ccagaatcaa gtaattcttg ttgtttaaaa gctctaccat tcaaaaaaac taccacttcc
     3721 cccaacttcc gctcttccca atctcccgta aatcctggaa aacgaacttc tggcactttt
     3781 gctccgtttt tagggaacat tttttgtaag aatccttttt tcgtttcctt cagcaaatct
     3841 aattcgtgct ggtgaagcat gatggtgtga tcaagttgtt tgaaaaatga accgatcttg
     3901 atctgttcgt cataagttgg aagtgagata ggtgtctttt taccactatc aattgtatag
     3961 gttccgacgg ttccaatgcc tgcaacgtca cggatatact tctgaatttt gttatttatg
     4021 aacgagtagt aaaagaagtt tccgtcaatt ttatgctcat tcttgaacca aatgatgttt
     4081 ccatccttaa agtaaattgg gttatcatca gtgattaata aggggacacc tattgatccg
     4141 acaccagtta caagcaagtc tccttgagaa actttacctg aatttttcga atattcatta
     4201 tatttttctt ctgaaatgta aagataatcg gatggttcct cattttttgc agctgagaca
     4261 atatcacgag cacgtaaaaa tctaatacct gagttagtcc aatctgattg atgaattcgc
     4321 ttcacagaag taacatccat catatctcct aacttacgct cttcccaatc ttccgtaaaa
     4381 ccaggaaacc ggatttctgg tcgtgtatca ttgctcatgt ttgaacacct ccagcgcacc
     4441 ttgtaaccac gcttcatttt caggtgttgt ttgcagggaa gagatagctt ctaaaagttc
     4501 tttttctagc acttgctgtt cttgtcgtat cttcttgatg tcatttccga catgaaccat
     4561 atcaattggt tcttcttctt cgaaagtatc gacatagcgt gggatattta agttatagtc
     4621 attttctttg atttcatcaa aggttgccaa atgagcgtat ttctcgacat cttttctttc
     4681 agcgtaggtt tcgaggattt tttgaatgtt ttcttccgat aatttattct gatttttccc
     4741 tttaacaaat tcacgactag catcaatgaa taatacatct cgagtttgac gattcttctt
     4801 caatacaatc accgtcgttg gaatcgatgt accaaagaac aagttcgcag gcataccgat
     4861 cacagcataa atactgccgt cttctaataa tttttgacga atgacccctt ctgcagcacc
     4921 acggaataaa accccgtgtg gcaagacgat agccatagtc cctgtttcct tcaaatgata
     4981 gaaaccatga agaagaaacg caaaatctgc tttggatttt ggcgctaatt tcccgtagcg
     5041 attaaaccga gaatcatcta agaaggtcgt atctgctgac caattagccg aataaggtgg
     5101 attcatcact actgcatcaa atgtgtaagg ttcatctgtt ggccagtcct tattcaacgt
     5161 atccccattg cggagattca tttcttcggc atctacccca tgtaaaatca agttcatttt
     5221 tgctagattg tatgtcgttg tatttagttc ttgcccatga tatttaacgt tatctggatg
     5281 agttaagtag ttacgtacat ttaacatcaa ggaacctgaa cccatggttg ggtcaaagac
     5341 actaaagaat cgtcgttctt tttgatccaa cgtcacgatt tgtgccatca tatcagaaac
     5401 catatgaggc gtgtaaaatt ctccggcttt cttccctgct tctgaagcaa attgactgat
     5461 cagaaattca tacgcatccc cgatcacatc cccatcgtga cctaaaactt ccacgtcatt
     5521 caatttcttg ataacttcgg taatcgtcac atttcgttgt tgctcatctg ttcctagttt
     5581 ttttgattgg agatccacat catcaaataa cccattaaat tgattgtagg tagatgccaa
     5641 ttgcacaaag gctttgttta gatcgttcaa ttggaacgtt gcttgttttg cttgatccgc
     5701 taagacgttg aaaagatact ctggtgagat cgaatacccc aaaatatcaa cgattgtggc
     5761 aatcaggtct tcttttgatt cttcatctga aagtaattca cgatacaaca ttgtttgttt
     5821 gcttactgtg tcatattcct ctaaggattc atctgctaaa agaaccactt gttccaacaa
     5881 acggtcggat aaatatttat agaaaatcag tcccaataaa taatttttat attctgacgc
     5941 atccatttta ctacggagat tatctgccgc actaaataat ttttgattta attctgcacc
     6001 caaaaagata cactcctatc tcttatttaa agttgctata taccctctta ttttagcata
     6061 ctccctttta gaaaaagcaa aaaaaaaaga aaaaatctta ttcttgataa caagctacgt
     6121 gacgtatatg cctaacaaaa atagaacttt tgaatcaatc ttaaatgaag gtttaaccac
     6181 agaaccggca tccattattt ttgatgaagt tatcgatgac gttgaagata gtaagtatca
     6241 taaaaattta aaagaacttt tttattttgc actcaacaat ggaccaagtt tgttcactgg
     6301 tatatcagta cctgatagtg ataatccatt aattatggca aaaatttact taacaaaatg
     6361 gtgtaataaa tacatcaggg ataggaataa cccagcatta aaaaaacctc ttaaaacatt
     6421 tggcgaaaaa gatgctgctt taactactag agtagcagca aatgctaata ttgatgatca
     6481 aaaagtattg aatgactact tgcgtggaca ttttttatat atgagcgctg agaacatgaa
     6541 cggaagtatt ttagaggaat atttagccga agtgttagaa cctgaaggtt ggatttggtg
     6601 cgccggctct gtatatcgag ctgttgattt ctgttattta ggaacctcac caattctttt
     6661 acaagtgaaa aataaataca atacagaaag cagttcgagt tcagctataa gagtaggaac
     6721 aacaattaga aaatggaatc gtttaaataa gtcgacaaaa atttctggtt tggatagccc
     6781 tatccccaat tggaaagcat taatagaaat gactgaagct agtaaggaac tggcagctaa
     6841 gttaactgaa aatagttatt tagcttatat taatgaaaaa tccacacgag aattatggac
     6901 actcgatgac taacaatatc cttattagta tctatccacg atacatggaa aaaattataa
     6961 acaaacaaaa aaactttgaa tttagaaatt tctatcttac ctcagatcct tataatatct
     7021 tttggattta cgaaacaatc cccaccaaag ctttaaagta taaattgatt gtaaaaaatc
     7081 caattactaa acttaagaaa aatcaacact actttttagg agacgatgag tttgaacaat
     7141 tagtttaaaa g
//
LOCUS       DACDXE010000108         7122 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.109, whole genome shotgun sequence.
ACCESSION   DACDXE010000108 DACDXE010000000
VERSION     DACDXE010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.109"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..716
                     /locus_tag="IWM23_002664"
     CDS             <1..716
                     /locus_tag="IWM23_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885254.1"
                     /translation="KNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLKKRTPLYNKS
                     YNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKYIFFTSVYDF
                     EGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSSIPWEAIQLS
                     GDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAYDIERLLKQK
                     TMKNILKNVKIAESLDDIL"
     gene            713..2134
                     /locus_tag="IWM23_002665"
     CDS             713..2134
                     /locus_tag="IWM23_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAP9885255.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            2227..2886
                     /locus_tag="IWM23_002666"
     CDS             2227..2886
                     /locus_tag="IWM23_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HAP9885256.1"
                     /translation="MKIVAIVPMKLNNKRLPQKNTKAFTNGKPLCYYILSTLLKVKQI
                     DKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQVLAGFTKEVEADVYVMTHTT
                     APFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGEPFNYELDNIPRTQDLPPLY
                     EETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDIDEAEDFMIADAIYNHLILQG
                     S"
     gene            2905..4488
                     /locus_tag="IWM23_002667"
     CDS             2905..4488
                     /locus_tag="IWM23_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl-CoA lyase"
                     /protein_id="HAP9885257.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            4623..5201
                     /locus_tag="IWM23_002668"
     CDS             4623..5201
                     /locus_tag="IWM23_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885258.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            5147..5665
                     /locus_tag="IWM23_002669"
     CDS             5147..5665
                     /locus_tag="IWM23_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885259.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            5673..6713
                     /locus_tag="IWM23_002670"
     CDS             5673..6713
                     /locus_tag="IWM23_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HAP9885260.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            6962..>7122
                     /locus_tag="IWM23_002671"
     CDS             6962..>7122
                     /locus_tag="IWM23_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885261.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         2609 a          822 c         1348 g         2343 t
ORIGIN      
        1 ataaaaatgt acaggtatca attttagaag aaggtatttt aagctatgga gttgacatta
       61 ttccaacagc aagaacaaaa gctatcgcag tactcaatta cttaaaaaaa aggactccac
      121 tatataacaa aagctataat ttttattgtt tttttcctaa tatatataca ggaagagaga
      181 atacaataca aattccatta gttacagctg ggtcaagaac cgcattgcaa ctttcggata
      241 tttttaaccc tatgatagat atttataaag aaaaaaaata tatatttttt acgtctgttt
      301 atgattttga gggcggagaa cctgtcggag aatatgaatt agtatgcaaa atagcagact
      361 tagtaggaaa agataatcta attgttaaga cgcatcctag agatgtaaga acaatttata
      421 aagataatgg atttatcgag gataaaaatt catcaatacc ttgggaggcg attcaattgt
      481 caggcgattt taacgataaa atatttatga caattaattc aggaagtgtt atttcaggta
      541 gtacaatgtc tgcaaaacct gtacgcacgt attatatgta taaattgtgt aacatagaag
      601 gaaatccgtc ttgcaaaaag aatgcgtatg atattgagcg attgcttaag caaaaaacga
      661 tgaaaaacat tttaaagaat gtgaaaatag cagaaagctt agatgatata ctatgagcaa
      721 aactaaatat ttaattaaaa atatgggaat attaacaata agtaattttt cttcaaaaat
      781 tttagttttt ttgttagtcc ctttatatac aagtgtatta tccacaacag aatatggcac
      841 atatgatttg acaatttcaa cagccacatt attgtttcca attttaacct taaacattgt
      901 agatgctgtg atgcgctttt taatggataa agataaaaat aaaaaaaata tagcatctat
      961 agcgattaaa gttatatttt taagcgtgat agtctttgga tccgtaatgt atttgttaaa
     1021 tattacaaaa ttatggcaag atgtttatgg attagaaaaa tatatttttt tgtattatat
     1081 atcctatgta tggaatcaat ttctaataca atttgctaaa gggttagaaa aagttaaagc
     1141 tatgggcata gcaggtatta tcagcacaat tgtaacaata attgccaata ttttcttctt
     1201 gcttattctg aaatggggat taattggttt tttcattgca aatattttat cacagattat
     1261 ttcagctact tatctgtcag tattagtgag aatatgggaa tatatcgatt ttgaggctaa
     1321 agatcctcag ctaaagagag aaatgctaaa ttactcgatt ccattaattg caactgttgt
     1381 tggatggtgg attaatagta cagctgatag gtatgtggtt tcctttatag taggtgtctc
     1441 ggcgaatggc ttattgtctg tggcatataa aatacctcaa ataattaata ctttgcaggg
     1501 aatatttata caggcctggc aaatttctgc aattaaagaa tatggagata atgagactgc
     1561 aaaattttat ggaaatacat ttgttatgat taatttgtta atgtgcataa cctgttcaag
     1621 tcttatcata ttaactagac cgttttcaca tattctttat gcgaaagact tttatgaagc
     1681 gtggaaatat gttccatttt tattaatagc taatgtttta aattgtgcat caggtctgtt
     1741 aggacctatc ttatcagcaa aaaaagattc taaatccatg atgtggtctg caatcatagg
     1801 ggcattagtt aatgtgatta tgagtgtagt ccttgtttat aagatagggg ttcaaggtgc
     1861 cacaatagcc actgttatat gttcatatgt aatatatgtt gttcgtaaac gagctgtggg
     1921 aaaagatatt tttataaagc attatcatat tgttgtggaa tcttggggat tattgtttgt
     1981 tcaggcaatc atagaaatat attttaaaaa ttatgtaata gaaattataa ttatgattat
     2041 tttaattatt ataaatatca gttatattaa gcagttgtgt tatagaggaa gaaacattat
     2101 ttcaatgatt aagagtcgag agcacaaacg gtagacgatg ttatttttat aaaaagatat
     2161 aatttattaa tggtcgataa ttatgaaagg aaaaatattc ttaaaaaaaa gagtatagga
     2221 tgtcgtatga aaatagtcgc tatagtacca atgaagttaa ataacaaaag attgccacaa
     2281 aaaaacacga aagcttttac caatggaaaa ccattgtgtt attatatact ttcaacactt
     2341 ttaaaagtaa aacagattga taaagtgtat gtctattgta gtaacccaga tattaaagaa
     2401 tttataccag aaggtgtaac atttttaaag agatctagtt ccttagatca ggatacaacg
     2461 agtattacac aggtattagc cggtttcaca aaagaagtag aggctgatgt atatgtaatg
     2521 acacacacaa cagcaccatt tattagcgaa aaatctattg aaaaaggact agatgccgtg
     2581 ctgacaggag aatatgattc tgcatttgct gcaaaaaaat tgcaagattt cttatggaaa
     2641 gacggtgaac catttaatta tgaattggac aatattccta gaacacagga tttgccgcct
     2701 ttatacgaag aaactagtgg attttatatt tataatcgca atgtgatgac cgagttgcat
     2761 agaagaattg ggaaaaaacc ttgcatagtt gaagtaggtg agattgagtc agttgatatt
     2821 gatgaagccg aggattttat gattgctgat gcgatttata atcatcttat tttacaaggt
     2881 agttaagaca gggggaaggt taggatgaac aatgtacaga ttttagattg cacacttaga
     2941 gatgggggct attgtaacaa ttgggaattt ggatttgata atacaaaaaa aattatttct
     3001 ggacttatag aatcaggaat tgagattata gaatgtgggt ttctcacaaa taaaacagta
     3061 tacaataaaa atgttacaaa atatacgacc ttagaagaat tagcacagat aattcctgaa
     3121 aaaagagaag gaaaattatt tgtagcaatg atgaactatg gcgaatataa tatagaggat
     3181 cttccagatt atgatggttc atctattgat ggaattagga ttgcgtttca taaaaaaaat
     3241 tgcaatgaag cgttgaaatt atgtcgtgag ataaagaaaa aaggctactt agtttttgtt
     3301 caagctatgg tatctctcaa ttatacagat gaagagtttt tagcagtaat aaaaaatgtg
     3361 aatcagttta aaccatacgc tttttatatt gttgatagtt ttggaatgat gaaaggaaaa
     3421 gatttaactc gtctttttta catgattgag cataatctag aaaaagatat acagataggt
     3481 tttcactcac ataataacat gcagcttgca tattctaatg cacaaaaact tataaccgta
     3541 caaacaaatc gaagcctgat tattgattct tcggtgtacg gaatgggaag aggagcaggt
     3601 aatcttaaca cagaactatt tgtagaatat ttgaatgata atgcaggaaa aaaatatcaa
     3661 ttaaaaccat tattatatat aattgatgaa atactaaata gtttctatca gaagagttat
     3721 tggggatatt cattaccaaa ctatatttca gcagttcata atgctcatcc taattatgca
     3781 agctatctgt catataagaa tactttaaca attgaagcaa tgaatgaaat attcgaaatg
     3841 atggatgaag aaaaaaaggt atcttatgat aaacaatata tagaagattt atactacaaa
     3901 tatatggaaa ctgggaaaat acaggaggaa tatatggaag aactgagaaa atttctctca
     3961 gacaaaaaaa ttctactgat tgcaccagga aaaagttctg tagatgaggt agaaagaata
     4021 aaagagtttg ttaccaaaga agaagtcgta ataattagtg taaattttga atattctcat
     4081 atagatatag attacgtatt tttaagtaat ttaagacgtt ttcatgaatt accgaaagaa
     4141 agcagaacta aatgtattgt tacatcaaat atacaagcat cggatgtata tttacggacg
     4201 agttataaaa agttgttatc tgaaagggaa gttgtaagag acaatgcagg attaatggca
     4261 attagatttt tagccgatat gaatgtggat gaaatattct tagctggatt tgatggatat
     4321 tcacataatg aagatgagaa ttatggtgag caatccatgg aaattataac aaaatttgca
     4381 ttattagatg caatgaacgt gcaaatgaga gatgaattgt cagaattagc taaaaaagta
     4441 aaggtgacat ttttaacgga tccacgttgg gttcgaattg ataagtagta gtaataatgt
     4501 ttttaaaatt ttgataaaga gatagtgaga gaataaaaat gggtaaagtc ttaaataaaa
     4561 aagaagaaaa atatttaagg gacgatgggg cgagatgaaa ctctaatttt gaacttttaa
     4621 gaatcatttc tatattgtgt attgttgctt accatgctaa tttgcgttta aattataatg
     4681 cagacggaat tatagtacat ggttcattaa ctgctaacca gattttcttg aattatattg
     4741 gtatttgggg tcagctagga gttaattgtt ttatatttat ttcggcatat tttttaagtg
     4801 agaaaagaaa cattcacttt gaaaatgttt tgggaattgt attagaatgt attttttata
     4861 gtgtattagt ttatggaatt ctttgtgcat gtggaataga aacattttca ttgaacagct
     4921 ttgtaaaaga aatattatca tttgctctag gtagatattg gtttattaca tcctatttag
     4981 cattgtatat tctaagtccg tatttaaata tcatgcttaa caatctggaa aaacttcagt
     5041 tgcaaaaaat aagtatgtta ttgtttatag gtgtatgctg ttatcgtatg ttatttaaaa
     5101 catctttgtt tggggattgt gctgttttaa ttgcactata tgtcatatgt tatacactga
     5161 aaaaaacgat aataatattt ttgagagaca cagaatcata ggaacaattg tatgtataac
     5221 agttatacca cttctcatat ccataggaat acttttcaaa tttataccaa atgcaatggt
     5281 taaccagatg gttcatattg gttctccatt gatggttata gcaagtatat ttttatttta
     5341 tgtatttaaa aatttttcat tttacagtaa gtatataaat aaacttgcga gaaccagttt
     5401 aggggtatat attttacatg aaaatcatta tttcattgat ggtatgaagt ctagaggctt
     5461 aatatgggat gggatatttg gattaggcaa aaggttttta gatagggatt tttggatttg
     5521 gtttataatt gcagttgtag ttgtgtgcat aatttgcatg gctatcgatg ttgtaagaga
     5581 aaaatttgaa agacagattt ttaaaattaa gagtgtaaag aacctgtgta aacttatgaa
     5641 tcatgttata aatatatacc cataattaat aaatgaggag aaaagaagtg aaagttatat
     5701 cattcggaga attattattg agacttgcag cacccggtta ttcaaagtta tttcaaagag
     5761 atagtttgga gactacattc tgcggagggg aagctaatgt agcggtatcc ttagcaatat
     5821 ttggatatca atcagaattt ataacaaagc ttccggattc agatgttgga tatgcggcag
     5881 ctaatgcttt gcgttatttt ggagtaaatg ttgataatat tatatttgga gagggacgca
     5941 tgggactgta ttatttagaa aaaggagcag ctcagagacc atctaaagta atttatgaca
     6001 gaaaattttc tgcaatttcg ttagctgata gtgatgaatt taagtggaag gagatcctaa
     6061 acgatgcaaa ttggtttcat tggacaggga taaatccagc attgtctgag aatctctttg
     6121 agacgtcctt agatgcatgt caggctgcca aaaaaagagg tataacaatt tcttgtgatt
     6181 taaattatcg atccaagctt tggacatcag aggaagcaaa agaaaagatg gaacagatac
     6241 taccattggt tgatatttgt atttgtaatg aagaagatgc agaaaaagtg ctaggaatta
     6301 aagctgagca tacaaatatt aatagtggag aattaaataa agatggctat gtgcaggtgg
     6361 ctagggagat tcacaagaag tttggatgta ggtatgtagc tacaactctg cgtagaagtt
     6421 attcggcaag tagaaatggt tggagtgcaa tgttatacga agcagaaaaa gataaagcgt
     6481 atttttccac agaatacaac atacaaattg ttgatcgcgt tggtggtgga gacagctttt
     6541 ctgctggtat aatatatgcg ttatgtgaaa atatgtccgg acaaaaggct attgaatttg
     6601 cgactgcatc tagttgttta aaacatacta tagaaggtga ttttaatcga actacaattg
     6661 aggatgtata taatttaatg aatagcggtg gaaatggtcg cgtgttgcgt tagtcaacat
     6721 ataaagataa atcacagtaa tttttatggt gattatctgt cttagtagtg attagaataa
     6781 cgtgaaaata aattgttgag cgtttatact gttttgaaaa ataaatttaa catattgaaa
     6841 aatacaaata tatacgtata ttataaagcg ccgattgtaa aattaagcta gacaactaaa
     6901 acagtcattt gtgctacact caaaatagtt ccgaccaaag aattataagg agtgagtgca
     6961 aataacctat acctatctta caacggatga acttgtaata atagagtctt atttgaaaat
     7021 taaccaatct gttgctaaaa ctgcgcattg cttgaatcgt tcaagtcaaa cgatccataa
     7081 agtatatctg ttcttcaagc aaagaaaatc agccttagaa ta
//
LOCUS       DACDXE010000109         7046 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.111, whole genome shotgun sequence.
ACCESSION   DACDXE010000109 DACDXE010000000
VERSION     DACDXE010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.111"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            94..1248
                     /locus_tag="IWM23_002672"
     CDS             94..1248
                     /locus_tag="IWM23_002672"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAP9885262.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="IWM23_002673"
     CDS             complement(1270..2022)
                     /locus_tag="IWM23_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAP9885263.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="IWM23_002674"
     CDS             complement(2386..5052)
                     /locus_tag="IWM23_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9885264.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="IWM23_002675"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="IWM23_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAP9885265.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
BASE COUNT         2072 a         1455 c         1277 g         2242 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacct
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttc
//
LOCUS       DACDXE010000110         6643 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.112, whole genome shotgun sequence.
ACCESSION   DACDXE010000110 DACDXE010000000
VERSION     DACDXE010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6643
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.112"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(1..859)
                     /locus_tag="IWM23_002676"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="IWM23_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM23_002677"
     CDS             1102..1557
                     /gene="tnpA"
                     /locus_tag="IWM23_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HAP9885266.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKIFRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1822..1920
                     /locus_tag="IWM23_002678"
                     /pseudo
     CDS             1822..1920
                     /locus_tag="IWM23_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2143..2373)
                     /locus_tag="IWM23_002679"
     CDS             complement(2143..2373)
                     /locus_tag="IWM23_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAP9885267.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(2603..3421)
                     /locus_tag="IWM23_002680"
     CDS             complement(2603..3421)
                     /locus_tag="IWM23_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAP9885268.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            3582..4271
                     /locus_tag="IWM23_002681"
     CDS             3582..4271
                     /locus_tag="IWM23_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAP9885269.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            4285..5787
                     /locus_tag="IWM23_002682"
     CDS             4285..5787
                     /locus_tag="IWM23_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9885270.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            5800..6276
                     /locus_tag="IWM23_002683"
     CDS             5800..6276
                     /locus_tag="IWM23_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAP9885271.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(6373..6483)
                     /locus_tag="IWM23_002684"
                     /pseudo
     CDS             complement(6373..6483)
                     /locus_tag="IWM23_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2172 a         1032 c         1204 g         2235 t
ORIGIN      
        1 acttgtcctc gataagaatt gtagcgccgt gatttacgtg tgtatgtcgt tacttttatt
       61 cctagcttct tgatcaggcg ctgaactttc tttttattga tagagaaacc gcggtttctt
      121 agttcattgg tcatccgtaa acagccataa tccttgtgct tctcccgaac tttaagcatt
      181 tccgcttcaa tctcttgatc tggattttta cggtccaatc gtttttgcca gtacataaag
      241 attgccttag ggaactttaa agtttctaga agctcaacta atttaaacgg tcctcggagg
      301 cttgcgatga ttcgtgcgat tgcttcattc gtcgttgttg ggcttcctgt tttcgcagct
      361 tcctcaattc ttttaaaaag gcattttcaa tttgaaggga acggactcgt ttctccaact
      421 cctcgatgcg ttcacgttct ttagacgttg ctttagttgt agattttttc ttatttttgg
      481 gttcattttt cttagacaag ataggtggac atcctttcgt ttttgagagt ccatcgatcc
      541 caccctcacg gaattgacgc atccaactag cgatcaaatt agggttgttc atgttaagtg
      601 tgttggcaac ttcacgatag gacagctcac ttgttagata taactctatc gcatccagct
      661 taaattgaac agaatatttt ttcttttgtc gctttcttac tagaccctct tcaccaaatt
      721 cttgataggc attgatccag cgcttcactt gtgatggagc cttaatggaa tatttctttg
      781 ccagataatc acttccgcct ttaccatcca agtaatcatg aacgatcttt aatttaagtt
      841 caaaagtata cttagccata aaaaacaccg acctccaaaa gttagatttt ttggtctaac
      901 ttttgggggt cggtacaaaa cttaaaagtt ttagaccctt ttattttctt aatgacttca
      961 gtcagttttg tgcgtaaagc gtgcgaaaca agaaaaccgc gcgtttctac gcgtggaaga
     1021 agtatcttta gcgataaaaa ctcctttcga tatactagtg agtggctacc aaccgctcat
     1081 cagatcgaaa ggagtttttt catgtcaaat gacgataaaa gtttagcata tactcgttgg
     1141 aattgtaagt atcatattat ttttacacca aaatatcaac gaaaagtaat ctatgggcaa
     1201 ttacgaaaag atcttggaaa aatatttagg aagttgtgcg aaatgaaaga tgtggaaatc
     1261 atagaagcac atgcaatgct agatcatatt cacatgcttg ttcgagtacc accaaaaata
     1321 tgtatatcca gttttatggg gtatctgaaa ggtcgtagtg cggtactaat ccacgagcag
     1381 cacgcaaatc tcaaatataa atacggaaac aaatcctttt ggtcgaagag atactatgtg
     1441 agtacagtcg gattgaatca aaagacaaat gaaaaataca tccgtgaaca agaagctgat
     1501 gatcgcattc gatatattat aagtaaaaga gagtacgtag aaccatttaa aaaataaaga
     1561 gaagtaagtg agtgaagatg agcagttggc ggtcttttaa aaatcccctt ttaaggggca
     1621 gcgagtgtcg accccttcta gggggattaa aaatacaccc gtcctcacgg gtgtatgggt
     1681 tactgataga cttgatttta actggtgtca gaaacttatg tccagaaagg gtgatgaaat
     1741 tttggcaatt tacggctatt caagaattag cacaagcaac caagattaca aaacacaaat
     1801 tcaaacatta gaaaatgctg gcgcagaaat aatcttccta gaaaatcact ggattatcga
     1861 acacctttgg aagtattttt gagttacgta agtattgatg atctgtctaa cttaatttga
     1921 caattaagag tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat
     1981 gaactaataa ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc
     2041 ataaactttt aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca
     2101 taacattgaa gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt
     2161 cttataaaca agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt
     2221 tatagaaaac attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc
     2281 agtaggttcc tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt
     2341 atttaagaga ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta
     2401 cacgtttttc tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc
     2461 aagaaccgta ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa
     2521 taagctatca agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta
     2581 ggtttagcta agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt
     2641 tttctcttaa ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat
     2701 cacaaatata aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa
     2761 tggctgtttg taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta
     2821 tagcaataat ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg
     2881 ttttcccaga taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt
     2941 gggcttgaaa atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat
     3001 tttctaaatc aagactagta tttccactag atcccactcc aaatatatat atgttttttg
     3061 attgagtgat cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg
     3121 aatagagagc ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat
     3181 gttctttttt ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag
     3241 aaaaaccaat tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct
     3301 taatatcact catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga
     3361 cttttttttc agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca
     3421 tacaaactcc tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact
     3481 cctaaatact ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag
     3541 ttgttatcca aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt
     3601 tagatcttat ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt
     3661 acaaagagga agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag
     3721 ttggtatacg tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac
     3781 caatgattgg aattattaaa agagattatc caccagaatc tccattcatt actgccacta
     3841 tgagagaagt agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc
     3901 ttagaaatcg acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat
     3961 atcctcatca gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata
     4021 aagcaggaat agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac
     4081 aagatgggcc agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg
     4141 ctgagggaag gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag
     4201 gtattgttgt gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag
     4261 cattaagtta gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc
     4321 atgttaccaa ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta
     4381 acaaatgaga gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt
     4441 ttaagtatta tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca
     4501 attggtattg ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga
     4561 gtagcttatc tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa
     4621 gatgcaacac tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt
     4681 ttacacaatc gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca
     4741 agatttattc cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta
     4801 atttggccac caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt
     4861 agtttaggaa cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac
     4921 cacaccattt accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga
     4981 tcaacaattg ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact
     5041 ggtctgttta cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga
     5101 ttaccagcag catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat
     5161 ggtgggcttt attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt
     5221 gaatatatgt ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga
     5281 ttatcttttt attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt
     5341 ttgatcgact ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa
     5401 ttaattcctt ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc
     5461 aaaaaattta aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa
     5521 gtaataaaca aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt
     5581 ggaaaagaaa atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg
     5641 aatggggaga ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa
     5701 gtgaagggtg gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc
     5761 aatcaaatat taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag
     5821 aaaaaacaag aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta
     5881 aatgatcctg tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt
     5941 tcaaaaattt atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct
     6001 attaatataa aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt
     6061 gaattacaag gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa
     6121 acctcgttgg ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt
     6181 atagttgttt ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt
     6241 gctcatggga attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa
     6301 aataagctaa ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt
     6361 ctgttcattt ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt
     6421 aaagttataa aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt
     6481 caaatataaa atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg
     6541 tttactcaac ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga
     6601 accttagcac tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DACDXE010000111         6636 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.113, whole genome shotgun sequence.
ACCESSION   DACDXE010000111 DACDXE010000000
VERSION     DACDXE010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.113"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            127..579
                     /locus_tag="IWM23_002685"
     CDS             127..579
                     /locus_tag="IWM23_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885272.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="IWM23_002686"
     CDS             593..1282
                     /locus_tag="IWM23_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885273.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="IWM23_002687"
     CDS             1407..3758
                     /locus_tag="IWM23_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAP9885274.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="IWM23_002688"
     CDS             3838..4233
                     /locus_tag="IWM23_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885275.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="IWM23_002689"
     CDS             4558..5175
                     /locus_tag="IWM23_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885276.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="IWM23_002690"
     CDS             5216..5515
                     /locus_tag="IWM23_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885277.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="IWM23_002691"
     CDS             5549..5716
                     /locus_tag="IWM23_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885278.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="IWM23_002692"
     CDS             complement(5761..6366)
                     /locus_tag="IWM23_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885279.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2282 a          925 c         1298 g         2131 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt tggttctgtt gcgaagtttg aaaatcaaac
     6601 gcgccctctc tgaactccaa atatcttagg ctgtta
//
LOCUS       DACDXE010000112         6432 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.114, whole genome shotgun sequence.
ACCESSION   DACDXE010000112 DACDXE010000000
VERSION     DACDXE010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.114"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(99..1229)
                     /locus_tag="IWM23_002693"
     CDS             complement(99..1229)
                     /locus_tag="IWM23_002693"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAP9885280.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IWM23_002694"
     CDS             complement(1878..3080)
                     /locus_tag="IWM23_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9885281.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IWM23_002695"
     CDS             complement(3140..3343)
                     /locus_tag="IWM23_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAP9885282.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IWM23_002696"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IWM23_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IWM23_002697"
     CDS             4880..5692
                     /locus_tag="IWM23_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9885283.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1930 a         1313 c         1008 g         2181 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcataga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatattttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt ca
//
LOCUS       DACDXE010000113         6375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.115, whole genome shotgun sequence.
ACCESSION   DACDXE010000113 DACDXE010000000
VERSION     DACDXE010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.115"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            37..1059
                     /locus_tag="IWM23_002698"
     CDS             37..1059
                     /locus_tag="IWM23_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAP9885284.1"
                     /translation="MIAELKPKHERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQLKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPREYKVIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNASFGQQLSVNDSIEVISLKDICREVAKELKDCERSNGTYDFDRFYQGKGD
                     INSQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIHQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1070..3274
                     /gene="topB"
                     /locus_tag="IWM23_002699"
     CDS             1070..3274
                     /gene="topB"
                     /locus_tag="IWM23_002699"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAP9885285.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVTAQNGIFDAKLDPNETQATQEAFEAYLKEKGVQVGKQPGTILEVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EHAYLLDHLDEYKHFLKAESFPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYEK
                     LTPLQQAIYDQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGETVTVDLQIFEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLTKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQATDFGATLQEVKV
                     ASEKQEAARHLGSCPKCQEKEVILYQKVATCTSEACDFKLWVTIAKKKLTATQLKEII
                     QNGRTSQPVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTTYKRKVRRKTS"
     gene            3520..4113
                     /locus_tag="IWM23_002700"
     CDS             3520..4113
                     /locus_tag="IWM23_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAP9885286.1"
                     /translation="MKDKVKCIFEKYNGNLSLKDARNEGLSPMTLDRLAEKDKIEKIA
                     SGFYTLKDSFVDELYLAQSIYTRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     NLDQYAIQPHYTNKENFELGITEAESFYGNKIFVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMNILKSPKEMTLYMLPLY"
     gene            4129..5028
                     /locus_tag="IWM23_002701"
     CDS             4129..5028
                     /locus_tag="IWM23_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAP9885287.1"
                     /translation="MTPTQFKAKTRNVAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTAAPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAFLENARVPFKLDITSGDSIIPAALIYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGIDNSRMKDFYDLYMIPKLERIDEINLYKSVQGTFKE
                     RNNQSVFKNLYNQEMPQLMKNEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEEVERKQNFKRSQQMNQEMER"
     gene            5031..5516
                     /gene="ssb"
                     /locus_tag="IWM23_002702"
     CDS             5031..5516
                     /gene="ssb"
                     /locus_tag="IWM23_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAP9885288.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVTTFTLAVNRNFTNQSGNR
                     EADFINCVIWRKPAETIATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            5882..6142
                     /locus_tag="IWM23_002703"
     CDS             5882..6142
                     /locus_tag="IWM23_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885289.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2314 a         1105 c         1313 g         1643 t
ORIGIN      
        1 cccgattggt tgattgatta tgcagaaaaa gaaggattga ttgccgaact aaagccaaaa
       61 catgaacggc aaaatttctt agttcgagac gatcgtttgg atcatgcggt cgccttttta
      121 tggaaagatc cacagacaaa agagacagta ggggcgagtt atcaaggaac gttcattgat
      181 tatgaacgtt ttggtgagag aggcacatat aagcatatag ataaaaattc gacagcgaat
      241 catggcttta acctaaagat tggtgatcct aaacagttaa aattttttga gagtagtatt
      301 gatttactta gctacgctgc actgaatcgt gatcagttaa acgatacgtg gttagtctct
      361 atggaagggt taaaacacca tgtgatcagt cattattttg gtgaggcagt atctgaattg
      421 agaaagaaac aagcctttcc tcaatccatc gagatttgtg tggataatga ccgtgcagga
      481 catattttct acgaaaaaga acaattaatg ggcgcagttg atccatttac gaatcagaaa
      541 gtacgttgtg aacgaggaat cgctaatgat tggcaagtgc caagagaata caaagtcatt
      601 tatgaagaag tcgcaaaaga agaaaaggtc acaccagaag ctattatggc catacacaag
      661 acagaaaaca atctgcaatt aacgaaccaa ctggtttccg ctcacaaagt caatgcgtct
      721 tttggtcagc agctttctgt taacgattca atagaagtca ttagtttgaa agatatttgt
      781 agagaagtcg caaaagaatt aaaagattgt gaacgctcga atggtacgta tgattttgat
      841 cgtttttatc aaggaaaagg ggatattaat tctcagattc ttttctctta taaagcagaa
      901 caatactaca aaggctataa aaatcatgaa catgaatttg tacctgaagt gaaaaaggat
      961 tggaacgatc aattgaagca tgagatccat caacaggaaa ttagaaagca aaaacgtgcg
     1021 atgttgtttc aacaaggcag acaacaagaa agggagtgag atcgtcacga tgaaaactgt
     1081 cattttagca gaaaaaccgt cacaagcgaa ggcctatgcc gatagctttt ctaaagccac
     1141 ccgaaaagac gggtattttg aaatccaaga tcgattgttt tcgggtgaaa ctgtgatcac
     1201 gtatggtttt ggtcatttag tcgagttaga ttcgcccgat atgtacgatg aaaattggaa
     1261 acaatggtca ttagagcatt tgcctatttt ccctacacat tatcactatc atgttcctaa
     1321 agataagaaa aagcaattca acgtggtgaa acagcaactt caatcggccg acaccattat
     1381 tattgcgaca gatagcgatc gtgaagggga actgatcgct tggacgatca ttcaacaagc
     1441 aggcgctgat caaggaaaaa cctttaaaag actttggatc aactccttag aaaaagaagc
     1501 catttatcag ggattccagc aattacgaga cgcagaagag acctatccca aatttgaaga
     1561 agcacaagca cgccagatcg ctgattggtt gatcggcatg aacggcagcc cactgtatag
     1621 tttactcttg cagcaaaagg gcataccagg aagtttttcc ttaggcagag tccaaacccc
     1681 aacgctttac atgatttatc agctgcagga aaagattaga aacttccaaa aagagcctta
     1741 ttttgaaggg aaagcccaag taaccgctca aaatggcata tttgatgcga agcttgatcc
     1801 caatgaaacc caagcgacgc aagaagcatt tgaagcctat ttaaaagaaa aaggtgttca
     1861 agtaggaaag cagccaggca cgattcttga agtcgaaacc gagaaaaaaa gcgcggcaag
     1921 tccacgtctt ttttctctat ccagccttca gtctaaaatg aatcagctca tgaaagctag
     1981 tgccaaagat actttggaag ccatgcaagg gttgtatgaa gggaaatatc taagctatcc
     2041 acgaacagac accccttata ttacagaagg agagcatgcg tatctgttgg atcatttaga
     2101 tgaatacaag cattttttaa aggctgaatc attccctact cctattcata caccgaatag
     2161 tcggtatgtg aataataaga aagtccagga acattacgcg atcattccta caaaaacagt
     2221 tatgacagaa gcaacgtatg aaaagctgac acctttacaa caagcgattt atgatcaagt
     2281 tttaaagacg acggtggcca tgtttgcgga aaaatatacc tatgaagaaa ctacgatttt
     2341 gacacaagtc cagcagcttc agctaaaggc agtcggaaaa gtaccagtag atctaggttg
     2401 gaaaaagcta tttggcaaag agagcgacag aaaagagaag gaagaagaac cgctacttcc
     2461 taaagtaaca aaaggagaaa cggtgacggt agatctacaa atctttgaaa aagaaaccaa
     2521 gccaccacaa ccttacacgg aaggtacact aatcaccgcc atgaagacag ctggtaaaac
     2581 cgtggatagt gaagaagcac aatccatatt gaaagaagtg gagggaattg gtacagaagc
     2641 gacacgagca aatattattg aaaccttgaa gcaaaaggaa tatatcaagg tagagaaaaa
     2701 taaacttgtg gtgacgaata aaggaatttt gctttgtcag gctgttgaaa aagaaccgct
     2761 gttaacaagt gccgaaatga ccgccaaatg ggaaagctat cttacaaaga ttggtgagca
     2821 aaaagggaca caagctactt ttctagcaaa tatccaaaaa tttgtttccc acttgttgga
     2881 agtcgtccca ggacaaatcc aagccactga ttttggagca acgttacaag aggtgaaagt
     2941 agccagtgaa aagcaagaag cggcgcgtca tttaggatct tgtccaaagt gccaggaaaa
     3001 agaagtcata ctttatcaga aggtggcgac ttgcacgtct gaagcctgtg attttaaatt
     3061 gtgggtaacg attgccaaaa agaaactcac agcgacacaa ctgaaagaga tcattcagaa
     3121 cggccgaaca agtcaaccag tcagaggact aaaaggtcag aaaggttcgt ttgaagcaac
     3181 gatcgtttta aaagaagact ttacaacagg atttgagttt tctgaaaaga agaagaccac
     3241 ctacaaaaga aaagtgagac gtaaaacgag ttagaattga cggagggaaa aacgatgaga
     3301 aatacaacgc ataaagaaca agaaatcgaa aatcaaaaag ccaaaatacg ttcagagcag
     3361 gagaaaatgc gtgccagcaa acgaagacta cagcaattga agaatcaaca acggattcaa
     3421 aaagaacttg aattagatcg ataaatcctc ctgttgttaa tattaaacta acatgttata
     3481 gttatgttag tttaatatta acattcgaga gggaaagcga tgaaagataa agtcaagtgc
     3541 atttttgaga aatacaatgg taatctatcc ttgaaggatg ctagaaatga agggttatca
     3601 cctatgacgt tagatcgttt agccgaaaag gacaaaatag aaaaaatagc atcaggtttt
     3661 tataccttaa aagatagttt tgtggatgaa ttatatctcg ctcaaagtat ctatactaga
     3721 ggaatcttct ctcacgagac agcgcttgat ctttttcaag taggaacata tatccctaaa
     3781 aaaattaata tgatgtttcc taaaggatac aatgtttcga aagagaattt agatcagtat
     3841 gccattcaac cgcattacac gaataaagaa aactttgaac tgggaatcac agaggcagaa
     3901 agcttttacg gcaataaaat tttcgtctat gatttagagc gaacgttatg tgatatgtgg
     3961 aatcccagat ataaagcaag cgtagaagta aaacaagaag cattaaaaga atatatgaca
     4021 agtaccagca gaaatacaaa taagctaaga agatacatga atattttaaa atcaccaaaa
     4081 gaaatgactt tgtatatgtt gccactttat tgagaggagt aaaggtgaat gacaccgaca
     4141 caatttaaag cgaaaacaag aaatgttgca gctgagaaac atttaaaccc acaaattgtt
     4201 caaagatatt atatgatgga aaaattatta gaatgtattg ttgaatcgcc ttatcatgat
     4261 gattttgtat taaaaggggg atttctgatc ggatcaaaat acgggttaga aaaccgttca
     4321 accgttgata ttgatacgac ttatcgaaat tcgaaactaa caaaagaaaa attagaaact
     4381 gttttttctg aattaacagc tgctcctaca aaagatggca ttcggtttga attaaagggc
     4441 ttaacagaga cgagggaagc cgattattac ccaggttttc aagcaaagtt tattgctttt
     4501 cttgaaaatg cacgtgtacc ttttaaatta gatattactt caggtgattc tattattcca
     4561 gcagcattga tatatgaaca taaattgatg tttgaagata aaacaattca tattccagct
     4621 tatcctacgg aacaaatttt agctgaaaaa ttacatgcca cgtttagttt cggaatagat
     4681 aattcacgta tgaaagattt ctatgatctg tatatgatcc ctaaactaga acgtatcgat
     4741 gagataaatt tgtataagag tgttcaagga acatttaagg aaagaaataa tcaatcagtg
     4801 tttaagaatt tgtataatca ggaaatgcct caattgatga agaatgaggg attgagggag
     4861 aaatggaaaa gatatcagca agaaaactat tttgccaaag atatttcttt cgaatctacc
     4921 ttaacttctg ttgattcgct gatgcaatca attgtcgagc aagaggaagt agaacgaaaa
     4981 caaaatttta agagatcaca acaaatgaat caagaaatgg agagataaag atgatcaata
     5041 atgtaacttt agtaggtcga ttaaccaaag atcccgattt acgttacacc gcaagtggca
     5101 ctgcagtgac cacttttacc ttagccgtta accgtaattt tactaatcaa agtggcaatc
     5161 gagaagctga ctttatcaat tgtgtgattt ggcgcaaacc agctgaaacg atagcgacat
     5221 tagctaaaaa aggcagcttg atcggcgtcg tcggacggat tcaaacgcgg acttatgaca
     5281 accagcaagg acagcgtgtc tatgtgactg aagttgtggc cgataatttc caactattag
     5341 aaagtaaagc agcgacagaa agccgtatgc acgctgatca atcgtccagc agtccaagcc
     5401 ctaccacctt tgaacaacgg gatacagcga cgcctaacaa caatggattg aacgcttcac
     5461 agaatccatt tggtggacaa tcaatcgata ttagcgatga tgatctaccg ttctaagtag
     5521 cattttctat tggttgcatt tgctacggtt tatgttataa tttagacaac aaaaagaatt
     5581 ttcaatctat aaccaaaaac cactagctgg cgggctagtg gctttctttt acgtttctat
     5641 tataagaatt aacgggtagg cttactgtct ttcttggcaa atttacaatc aagccatttg
     5701 cagatatagt gcgcggctac ttctgccatg attgcaaaaa caaaatcaaa aagaattttc
     5761 aaacctataa cctcctcccc ttgccagatt aggccggggt agcagacagc aagactatta
     5821 tagcataatt gaaatatcca aatgattgga tttttgctat aattttagcg aggtgattgt
     5881 gatgaacaaa tatgttggtt tgcttgataa aatccgagta ataaaaacgc aaccgttatt
     5941 ggttcgcttt actttacaaa cgattcatga atcgattaat tgtgtagttg cagatattga
     6001 aattatcgat aaattgctaa ttatggatga tggaaaatac aatattgccg tcacaggtca
     6061 cttcaataaa cgaaatcagc tagttattgc tagcatgtat gttagaaatc ccgatcattt
     6121 tacgcgatcg atgggcatat aaataaaaga agagttgtag tcctaaatga ggattgcagc
     6181 tctttttgtt ttacatagaa acaggaaaag acctttttag gaagacacaa gtgccttctc
     6241 aattgaaaaa cctttctctc actccaaccg ccaccgccgt tcgagaagaa aggaagtgaa
     6301 cgctgcacgt tcactaagat atggtgtctt gcaaagcaag acaaaataga caaaacttcc
     6361 tctcaccaaa aggtt
//
LOCUS       DACDXE010000114         6303 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.116, whole genome shotgun sequence.
ACCESSION   DACDXE010000114 DACDXE010000000
VERSION     DACDXE010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.116"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            334..714
                     /locus_tag="IWM23_002704"
     CDS             334..714
                     /locus_tag="IWM23_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9885290.1"
                     /translation="MSEQHQNLASDHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVAPD
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            696..1610
                     /locus_tag="IWM23_002705"
     CDS             696..1610
                     /locus_tag="IWM23_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9885291.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1855..2544
                     /locus_tag="IWM23_002706"
     CDS             1855..2544
                     /locus_tag="IWM23_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885292.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2665..2751)
                     /locus_tag="IWM23_002707"
     CDS             complement(2665..2751)
                     /locus_tag="IWM23_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9885293.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            3427..4377
                     /locus_tag="IWM23_002708"
     CDS             3427..4377
                     /locus_tag="IWM23_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9885294.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            4398..4931
                     /locus_tag="IWM23_002709"
     CDS             4398..4931
                     /locus_tag="IWM23_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9885295.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            5567..6100
                     /locus_tag="IWM23_002710"
     CDS             5567..6100
                     /locus_tag="IWM23_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885296.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
BASE COUNT         2323 a         1102 c         1174 g         1704 t
ORIGIN      
        1 aaaaaaaaat aaatcgatac attttagaag aatagggttg tttgaatatt acaaagcaac
       61 aatttcacct tcggtgaaat aagcgaaact ttacaaaaag tggctagcaa atgctagcct
      121 ttttttgtat tgtggagcgt ggattttgag ggggattcaa gggggagctt gcgctccttt
      181 gacaagcggt tatttgcacc aaatacggag gtatttggct agcaaatata gagcttgcca
      241 aaccctttta gtgagcttta ctcattgtac ctttttaggt acaatggtat tgcgaacgat
      301 acgtccgtag gacgtactga aagggtggga cttatgagcg aacaacatca aaatttggct
      361 agcgatcatt ctaaaaaata tacctatcga tccgagccaa aacaaatcag ttttcgagtg
      421 agcgaatccg aatttgcaaa gctgaagcag tcagctgaag ctttgcaaat gagtgtgccg
      481 gctttcgtga aagccaaggc acaaaacgcg cgtctggtga caccgaaagt ggcgccagac
      541 attgcccagg caatcgcgcg cgatttagcc aaagctggag ggaatatcaa tcaaattgcc
      601 aagtggtgca atacacacca acacgacgtt gctcctgatg atgctcaacg tttatccgaa
      661 aacctaaaaa tcatgcaaaa ggagctacaa aagatatggc aacagttaaa gtaagtcgaa
      721 ccacttcatg tagccgagca atcaattacg cagaaccacg agcaaccgtc aaaacaggaa
      781 taaactgtga cattgattat gccaaaagtg aaatgaaaca aatacgcacg ctttatggaa
      841 aagacgatcg tgtacaagca cacttattga ttcagtcctt tcgaccagga gaaattaccg
      901 ctgaaaaagc caatcaacta ggggcagagt atgcgaaaaa aatagcccct aaccaccaaa
      961 tcgcgattta cacacataca gacaaagacc atattcataa tcacattgtc attaactccg
     1021 ttaatcttga aactgggaaa aaatttcaag cccatgggca agcattttta aacaaatgtt
     1081 atgacctaaa tgacgagatt tgtcttgaac atgacttaac aatcacggaa cgaggaaaga
     1141 aaaccgaaaa acgaaccatg tctgaaatta aattaaaaga aaaaaatgaa ccggtttgga
     1201 aagacgaaat tcggtttgcg attgatcaaa caatgaaaaa caaaaaaact cgaacgtata
     1261 cccaattttg tgattcactt aaaacattcg gcattcattg cttgaatcgc ggaaaaaatt
     1321 ttacttacga attggtccac aaaaagaaaa aagtacgatc caacaaacta ggaaaggatt
     1381 acgaaaagga gacgatttta catgagctgg acagacgaga acgaacctac aatcaacaac
     1441 gaaccaacga gctacgaaga gctcgagcta gcattcgcga aactgcagaa gaagtacgaa
     1501 gagaacaacg aacttttacg cctgaacaat cggaaattag accgactcga agaaaccatg
     1561 aacaacacgt tgaacgagga acagaccacc aacaagaatt atcgctctaa aacgttaaaa
     1621 gacatcgaaa acgaaataaa gagacaacgt gcctttcttg cgccttatac ccaagaaagg
     1681 atacaaacta cgatggattc acaagtcaaa aaactccacc aagcgctatt agaccagcga
     1741 aaccaagaac aaaaaaaggc tttaattcaa cgatcgatgg atattcttgc ccccttatgt
     1801 tgcttagtac taacattatt agccttttgg ttggtatggg gagaataaac agacatggca
     1861 ttctatttac cctatctact catttctgcc agtattttag gaattatttg gcttagctat
     1921 aaggcttatc aaacaagata ttcattaaaa ggctctaaaa ttcgatttaa gcgattttta
     1981 ttggtcgggg ttatatttgc cttatcaatc gttttaagtg gtttattcgg cgttttagag
     2041 ggtaataaac gtgtttcaag aagcatttta ttaggggata tcactcaaaa gtatgaagca
     2101 gcatgtgata aaaagaaaaa agagcaagca ttagcacaaa aaatggagga atttaccgct
     2161 tgttatgagg acatgaacaa tatttttgtg gatcaagaga aacgtttaac agataaaaac
     2221 atgaaaaaac tcactcgttt ttatcaaaac ttaccagaaa aacagcaaaa agaggtacaa
     2281 gaaaaatatg agcaagtaaa aaaagacgtg caatatttta aagatacgca gacagaagaa
     2341 gcttgttgta acttatttag cgatacaatc cctttttcaa ccagtgaaca agaacgaaaa
     2401 gaacggcaac aatcggtcac atacgaacgt tatgaaacac tgcttcagca agcaacaaac
     2461 atacaaaatc caacgaaaaa agaaacggca ttaaattatc tagaaagtgt caaggaatgg
     2521 ttggatcagc aacaacaaaa ttgaatttga ttagcaatca tgttaaactg aacctgcaaa
     2581 tgatcaaaca ttagatacac gaaagcccca gagaggtcgg attctctggg gctttttttc
     2641 aggttgatgt cgcctttatt gattttactt cttgtttcgg ttattcaacc aataactgaa
     2701 taaagagaca caaacttcta caagaagggg gcaaatgaac cgttcaaaca ttagatacac
     2761 ctccttccag tcgcaagact gtcggatagg cgacaagtta ttatacctta caaaataaaa
     2821 tctgtgtggt gtttttaagt atcccttatt ttcgggttgt tagggcttgc cctgaccgcc
     2881 tgtaaggcgc tttaagaaca agataaggtt ttttacccaa gactgtctta aaacgactta
     2941 gacgagctat cagaggcttc ccccacctcc gttactgact tgtttttagc aaaggagcct
     3001 ttaggcgact gcgcagtaca atgtgagagc atagcgatca cactttttta gcgaccgtag
     3061 ggagcaaggt tttgaattgg cagttttgaa aaatgcgcga acctgttggt agaaaaaaga
     3121 agtggttttt aggcgggaaa ttttgtcacc ctcttttata atctcttttt aaaccttttt
     3181 ttaaacctct ttaccgtcgg ctgtgtcctt actccttcaa tggatacagc gttttataaa
     3241 ctacattttg tctgctttta aactacattt tgtctgcttt taaactacat tttgtctgct
     3301 tttaaactac attttgtctg cttttaaact ctttttttgc actcacgtat gttataatat
     3361 gcattatcac agacaaaacg ttgtttataa cgtctgtatc cattgaagga gtaaggagga
     3421 atatctttgg caaatgaaat cgtgaaatac catcatgaat taaataccat tccgttgcgg
     3481 aaatttacac cagttgaaat gaatctattt ttctctattg tgtctagaat gcgcgaaaaa
     3541 ggcgataaaa cagtccgtct tacttttgac caactaaaag atttaagtaa ttataaagca
     3601 actgctaata atcgtttcat taatgattta gaaatgactt atgataaatt aatggattta
     3661 cgttttggta aaagaagtgc taatgggctc gatatagata gattcgttat gtttagtgag
     3721 tttaaaatac gtggaactgc taaagatcca tatgtagaca tacaagttta cgaaaaagct
     3781 ttacctttac taaacaattt agatgagtgg gttcgctatt cactccaaca atttaacgaa
     3841 ttacaaagta gctatgcaaa gactatgttt cgattactaa aacaatatcg aactaaaggt
     3901 tttgcttatt tttctaaaga ggattttcat gaactattag atattccaaa aagttactgg
     3961 aacaaccaag caaacgttga taatcgtgtt ataaagccta tacgacagga attaaccgca
     4021 atcttcaaag gattatcaat caaaaagaaa tacggaaaag ggcgaggaaa acctgtcgtt
     4081 ggctatcaat ttacgtttaa accagaaatc aaaaaagcag atgattttaa taaacatgca
     4141 ccaaccaaag aagaacaaaa taaagcattg gctttagctg ataaacaaaa agaatacgaa
     4201 gaacctgcta caacaattaa cgaaaaaatc aaacagaaga aacgagaact tgaactcctc
     4261 caacaaatca aacaactaga aaaagagaaa gaagctcttg aaaaacaaac agagtccgca
     4321 ctttcgaaaa aagagaaaaa aagtttatta tctgatttag ataatttatt ccagtagcaa
     4381 cctaggaggc aaccagtatg caaccacaag aaacctttac aataaaggaa ttgtcagaac
     4441 tttttaagat gagtaggcaa gcaatatcga aacatatcca aaaattagat tcttccatga
     4501 tagctaaaaa tgagcgtggt tataaggttg tattgcgttc tggagtattg caacttgctc
     4561 gcaacttagg caaccaaatg ttgctagata ggttgcacga gtacgaacaa gacaacgata
     4621 aaaatgttaa tataccaagc aaaaacgtgg ttacaaacaa gttgctagat cagttgcgtg
     4681 aagaaaatga atttttacga gaacaacttg ccctcagaga gcaaaataat tctcatatac
     4741 aaaaactact cgaccagcaa caacaactca ccctacaagc caacaaacaa atcgaaaaac
     4801 tgcaagaaaa actacagttg acttatacag aagaagctcc agagaatgac tcaacgactc
     4861 tttctgaagc aacagaaaca atagacaaac aaattgatac tgaaaaaaaa tggtggcaac
     4921 tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc ggttctattt
     4981 tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa gggaataaac
     5041 atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa ttgtttgtaa
     5101 aagaaacaga taaataataa aaaagactgc tctttcgagc agccagttaa aaaataatga
     5161 gtgtttattc ttgaaaaagc aacgcgctta tgaagcgcta ccaaaaattt atcatatttt
     5221 attaaaaata acaacccagt aaaaaatgac tggtaaaaat tcacaaagaa acggaaagaa
     5281 gcaaccgcag aaaatcaagc ttaaaaagag cttttgacct agaccaatcg aaaatcacaa
     5341 agtgagcgcc tgtatctatt gctacaggcg ctacttggca tagtgtacat tatgtcaaca
     5401 agaatttaat ataacatatt ttcctaatgc attctaacta taccacatta tcaaaaatat
     5461 aaattatctt tactaaataa aatattgaca agcctatacc ttttgtttta tgattttcac
     5521 aaagaaaacg ttttctatat atttttctgt atcaggagga aattctatga aaaaatttac
     5581 tttaggggta gctttgttaa ctattggggc agttgtttta ccgtcaacta ctatagttta
     5641 tgcttcagaa aattcccaag tggtatcatt aaacgaaaac aatgctactg aaaatgtagt
     5701 tattgagtac actgaagatg gtatggtcgt aactaaatat gaggatggtt atagtgcgga
     5761 agattttgca aattcttcag atatcatgac aagagctgca gttgcaagat ggggcaaaaa
     5821 ggaatataca aatattgctg taaccactgg agtagctgct aatgctatta atgctgcctt
     5881 ttgggcagga gggaaagcta gcacttctat gtttggtatc ccaggttggg caattggtgc
     5941 tttgttaaca ggtgcaagtt ggactaaaaa aggtgcttct ccagggaatg cagttgcaaa
     6001 agaatgggac aaaaatggta atggctggat tggattttat taccaaaggg gatatgatgc
     6061 agctggaaga gttgtagcta ctagatattc aacgttataa gttaaggagt gatgtttttg
     6121 atatctaaat cattaaaatc attaaaaaca tttactaaaa agaatccgca aatagctact
     6181 attcgtattt ttatatttgc tatagctgtt gctataattg ttaatatgct aagcggaggg
     6241 caattataca aatctggttt atttttagtg ttaatcttag ttgtcttaga cataataaag
     6301 tct
//
LOCUS       DACDXE010000115         6285 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.117, whole genome shotgun sequence.
ACCESSION   DACDXE010000115 DACDXE010000000
VERSION     DACDXE010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.117"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..177
                     /locus_tag="IWM23_002711"
     CDS             <1..177
                     /locus_tag="IWM23_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885297.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="IWM23_002712"
     CDS             190..690
                     /locus_tag="IWM23_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAP9885298.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="IWM23_002713"
     CDS             787..2634
                     /locus_tag="IWM23_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HAP9885299.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="IWM23_002714"
     CDS             2637..3533
                     /locus_tag="IWM23_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9885300.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="IWM23_002715"
     CDS             3583..3972
                     /locus_tag="IWM23_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885301.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6285
                     /locus_tag="IWM23_002716"
     CDS             3959..>6285
                     /locus_tag="IWM23_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885302.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLE"
BASE COUNT         2118 a          932 c         1347 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaa
//
LOCUS       DACDXE010000116         6174 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.118, whole genome shotgun sequence.
ACCESSION   DACDXE010000116 DACDXE010000000
VERSION     DACDXE010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.118"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..172
                     /locus_tag="IWM23_002717"
     CDS             <1..172
                     /locus_tag="IWM23_002717"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885303.1"
                     /translation="DEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFI
                     ADLYKENGVELK"
     gene            169..1473
                     /locus_tag="IWM23_002718"
     CDS             169..1473
                     /locus_tag="IWM23_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAP9885304.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1569..2438
                     /locus_tag="IWM23_002719"
     CDS             1569..2438
                     /locus_tag="IWM23_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9885305.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2550..2870)
                     /locus_tag="IWM23_002720"
     CDS             complement(2550..2870)
                     /locus_tag="IWM23_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAP9885306.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3040..3999
                     /locus_tag="IWM23_002721"
     CDS             3040..3999
                     /locus_tag="IWM23_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885307.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            4089..4484
                     /gene="dhaM"
                     /locus_tag="IWM23_002722"
     CDS             4089..4484
                     /gene="dhaM"
                     /locus_tag="IWM23_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAP9885308.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            4486..5469
                     /gene="dhaK"
                     /locus_tag="IWM23_002723"
     CDS             4486..5469
                     /gene="dhaK"
                     /locus_tag="IWM23_002723"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAP9885309.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            5497..6102
                     /gene="dhaL"
                     /locus_tag="IWM23_002724"
     CDS             5497..6102
                     /gene="dhaL"
                     /locus_tag="IWM23_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAP9885310.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         2073 a         1114 c         1339 g         1648 t
ORIGIN      
        1 agatgaaaaa ttgaaaaaaa gacaagaatt acttgaagaa gtgtatacgg aacgttttgg
       61 aacaaaagaa gagagagaaa cggtccgttt ttattctgtc tccgaagaga aaaatctaga
      121 tacgacattc attgcagatt tgtataaaga aaatggggtg gaactgaaat gatctcaatc
      181 acccatacag gtaatttatt cttagatact tgtttgtcta ttatgtattt ttttctcgtt
      241 tcttatccga ttttaggcgg atttgtatgg tttattggtg tctggtgtta tgtgttttta
      301 tacaaacata agcaaaaaga atgggtagat gttcccttga gtgtcgaacc gttcatcacg
      361 atcatggtgc cagcccataa tgaagaaatt gtgatcgaag atacaatcga gtatttgatg
      421 acgaaattga attatcacaa ctatgaggta ttagtgacag acgatggttc aacggaccaa
      481 acaccagaaa tattagctag actgatgaaa aaatacgcta atcttcgtgt ggttcggatc
      541 gaaaaaaata aaggaaaagc ccacgcgttt aatatcggac ttgcctttgc aaaagggaaa
      601 ttgattttaa gcaatgatgc agacacggta ccggagccag atgcactgat taggtatgtg
      661 aattatttca ttcgccctgg tgcaagacat attgctgcgg tcactgcaaa tatggatgtc
      721 caaaatcgta ccaaattgat tgcaaaatct caaacggttg aattctcaag tatcgtgggc
      781 atcattaaac gaacacagtc agctgttttc ggtggtcttt atgcgtacag tggagcaaat
      841 acgatgtatc gaaaagaagc cttgatcgat gtgggcggat tcagacaaga tcgagcgaca
      901 gaagacatca gtattgcttg ggatcatcag ttaaatgact ggttatctgt ttttgctcct
      961 gagattatct ttttcatgga agtacctgtg acgttgaaga tgctatatcg acacagaaaa
     1021 cgttgggcaa aaggtggaac agaagtctgg ctgacgaatt tcaaaaaagt gatgctccat
     1081 ccttttaaac atattggaag aacgatcatc ttcattgatc aaacattgag tattgtctgg
     1141 tcaatattct tttgtatttc cgtagttctt tttgctggat taatcgggca ttatgtatat
     1201 caaggaaact acgaacagat ttatattact tttacattca gttttgtgtt catctgcttt
     1261 gaaatggtcg caggattttt ccagttgcta gcttcgctga ttgtagatga tcgacgaaga
     1321 aaattgaaat accttctttt cgcaccacta tatatgcttt tgttctggat agtgaacgcg
     1381 ataacgatcg tgacgacttt cattccagca gtcaaaacga ttttaggtta tggaagcggg
     1441 acatggaaaa gcccggaacg tacaaaaaaa taaaagatgc accgtttttt agacaaacag
     1501 cctgttgtgt ttatggttct tccaaacatt tttgcttata ataagaaatt agttaaaaag
     1561 tagtaaggat gggaaaagtg atgaaaaaaa gaatgaagcc gttaatctgg ccaattgctg
     1621 tttccagtat tttcttattg ggacagacag cttttgcgga aacttctgaa cctgctcaaa
     1681 cagaaccttc aacaaccata agctcttctg gtgctgaaaa cggtgcctcg gaaacagaaa
     1741 tgacttccaa tgtaccgaca gataattcag gagaaacgat ttcatcttca gaagatacat
     1801 caataaatag ttctacctct tctgaccaaa tcgatcattt gcaagacttg gaaaataaaa
     1861 gaaaagaact cgaagaagca ttagaagctg caaaaaacaa attgaaagaa gaccaagcag
     1921 caatcgatca agatttagct gatcttaaaa ataaagtggg aaatgaccgt gcacaacttg
     1981 agaaaaagct tgccgaagcg aaagcaaaag cggaagctgt ccaaacagat cttgacggga
     2041 agatccaaca agcaaaagaa ttcaatcagc aattaaatca ggttgctcaa acagcttctg
     2101 gaaagctaag tcaaactcaa gcggatatca cagcggccag tcaagcaatc cacggaaaga
     2161 tcagcgaagc acaaacagtt gctaatgatt ttgaagcaaa aaaagctgac ttagcagcta
     2221 gtttccaacg aataaaagat attttgcatt cggcaggaga aaaatcagtg acctctggaa
     2281 gcacgacaga aacagttcag caaacgacag agccatccaa atcaacaagt cagactttgc
     2341 caaaaacaaa tgaaaaaaca gcttctgtca gttattcctt agcaggggcg ggtgtgttag
     2401 tcgcagcagc ttacggaatc atccgcaaaa agaaataaaa aagagaggat gggaaggtag
     2461 agtacctcga agttagactg aaaaatctaa ttcttgagtg ccactacatg tcccatctct
     2521 ctttagttat taaaacagat gtcccctact tatctcgtgg agacagctgt tccactacat
     2581 gtgaccatca gcatgccatt gtcgctgcct aatacttcat agtccatttt tacgccaatg
     2641 atcgcatcag ctcctaatgc ttttgcacgt tcggccattt cggcaagtgc ttcttctctt
     2701 gcacccatta gttcgttttc atagcttttt gagcggccgc caaagatatt tcgcaacccc
     2761 gcctccaagt ctttaaatgc atggatccca gtaatgacct caccaaaaac gatcccttta
     2821 taagctacta cggcttttcc ctctacactg ttcgttgttg ttgtaatcat tcgatcactc
     2881 ctctctttta attaaagtat aacaaacttc aagaagcact acacatattt tcccaaaatt
     2941 agaaatcgcc gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca
     3001 aaatagttcc gtcgaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca
     3061 ccaactgaac tcgtaatgat agaagcttat ttcaatcaat cacactctgt ttcaaaagta
     3121 gcccatctgg tacaacgttc tcgacaaacc atttataatg tctatcgttt tcttaaatct
     3181 ggtggaagcg caataagcta ctatgaacaa tataagaaaa acaaacgtaa ctgcggaaga
     3241 cgtccgattg tgttaccaga tgggcaacaa gaatacattc aaaaaaaggt ggctcaaggg
     3301 tggactccag atgtccttat tggtcgagca gagtttccta ttgcttgttc ggtacgtaca
     3361 ctatatcgga tgtttaaaga taaaaccttt gaccctcacg atttaccaat gaaagggaaa
     3421 agaaaaccaa acggacataa agaaaaaaga ggaaaacaag cttttcgccg ttcgattcag
     3481 gatagaacaa ctgattatat acagttcaat catgagtttg gtcacctaga aggtgacacc
     3541 attgtcggtg gaaaacataa aagtgcggtg atcacgttag ttgagcgatt atctaaagtg
     3601 atcatcacct tgaaacccgc tggtaggcaa gctatagata ttgagaatag actgaatcaa
     3661 tggtttcaat ctgtaccgaa acacttgttt aaatccatca catttgattg tggaaaagag
     3721 ttctcgaatt ggaaaaagat tagtaatacc aatgatattt cgatttattt cgctgatcca
     3781 ggaacacctt ctcaacgagg actaaatgaa cattcgaatg gcttattacg taaagatggc
     3841 ttatataaag ctatggattt taataaggta tccgaaacct ttatccaatc ggtggcatca
     3901 aaacgaaatc atattcctag aaaatcatta cactatcgca caccattgga agtatttttg
     3961 agttacgtag gtaaagatga tttgtctagc ttaatttgac aaacgaaaaa tttattatat
     4021 acttgaattg ttcattaatg aaagcgaaac caatcgttag caactatgta aaagaaagga
     4081 agattcttgt gaaaaaaagt attttgctta tttctcatag tcaaaaagta acagatggta
     4141 ttaaagaaat gattgagcag atgcaaaaat ccgaagaggt gcaaattttt tctctcggcg
     4201 gaataaatgg cgagatcgga tcagacccga tgaaaatcgt agaagcagtc aatgaatcaa
     4261 gcgatgcagc tagttatttt gtttttgcgg atatcggtag cgcggtattg aactcggaac
     4321 tggcaaaaga catgttggaa gaagagcagc agcaacgtta tcatctggtg gatgcgcctt
     4381 tagtggaagg agcttttgca gcagccatca ctgccggtat aacagatgat actcagcaaa
     4441 tcttaacaga agcacaaaat gccggaaaga aaggatggga ataaattgaa aaaaatcatg
     4501 aatgatccaa gcaatattgt cgaagaaatg ctggaaggat tagtaaaaag ctatccagaa
     4561 cttgttcatc gtgtagaatc ttcccgtgtt gtagccaaga atcagaaagc ggaacaagtt
     4621 ggtttggttt ctggtggagg aagcggacat gaaccatccc atgccgggtt tgtaggggaa
     4681 gggatgttaa gtgcagcggt gcttggtgat gtgtttactt ctccaactcc agatcagatt
     4741 cagacggcta ttaaggaagc cgatagtgga aaaggagttt tgctgatcgt taagaattat
     4801 acaggggatg cattgaactt cgatatggca aaggaattag ctgctatgga cgatattgaa
     4861 gtagaaagtg tgatcgtaga tgatgatatc gccgttgaaa atagtacgta tactgctgga
     4921 aaacgcggag ttgcaggtac tgtccttgta cacaaaatcg tgggcgatgc agcaagaaac
     4981 ggtgcttccc tagctgagtt gaaagaactg ggagaaaagg tcgtacaggc aactaagacg
     5041 atcggtctag cgcttcgagc agcaactgta ccagaagtag ggaaaccagg attcgaacta
     5101 ggagaagatg agatcgagta tggtgttggt atccacggtg agccaggcta tcgtcgagaa
     5161 aagatgcagc cttctaaagt actggcaaag gaacttgtta ctaagatttt agatgattac
     5221 tcgaatttac caaaagaagc aggtgtacta gtcaacggaa tgggcgggac gcctttgatg
     5281 gaacagttcg tgttcatgaa tgatgtttta gctctgttgg aagaaagagg cgtcaatgta
     5341 gttttccgaa aagtcggtaa ttttatgacc tcactagata tgcaaggtct ttctctaaca
     5401 ctgatcgact tgacggagac acaatggaaa gattctttag aaagcaatgt acagacgatt
     5461 tcatggtaaa aaatctcaag ataaaagggt gataatatga aactaaccgt tcaagaaatc
     5521 caagaatggc tcacgcaatt tgcagaggca atcaatcaga acaaacaata tctcagtgat
     5581 ctagataccc cgattggcga tggagaccat ggaaataaca tgggaagagg ggtttcagct
     5641 tatgaagaag cttttcaagc cgatcatcct gaaacaatca gcgatacatt caaagtattc
     5701 tctatggcga tgatatcgaa agtcggtggg gcttctggtc cgttatacgg atctgcattc
     5761 atgaacatga tgaaagcgac aaaagaggtc gattctattg attctcaaga aaaattaggc
     5821 gaagtgatcg agcagggaac agcgggtatt caaagccgag gaaaggcaga agctgacgat
     5881 aaaacgatgt tagatgtttg gctgccagtc acagaggcat tgaaggcagg gaatttaaca
     5941 aaagaaatca ttgaccaggc aaaagaacac accaaagtct tggtagctaa aaaaggacga
     6001 gcttcctatc taggagagcg tgcaatcgga catatcgacc caggagctgc ttctagtgcc
     6061 attttatttt caacgctttt agatgtgatc aatcaacctt aagttcaata aaacggctct
     6121 ttgtcaataa ggactgatgg gttgtacaaa atcaaatctg ggtcagaatg aacc
//
LOCUS       DACDXE010000117         6108 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.119, whole genome shotgun sequence.
ACCESSION   DACDXE010000117 DACDXE010000000
VERSION     DACDXE010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.119"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(182..1144)
                     /locus_tag="IWM23_002725"
     CDS             complement(182..1144)
                     /locus_tag="IWM23_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018375965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9885311.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="IWM23_002726"
     CDS             complement(1146..2585)
                     /locus_tag="IWM23_002726"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HAP9885312.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="IWM23_002727"
     CDS             2770..4716
                     /locus_tag="IWM23_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HAP9885313.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            4980..5852
                     /locus_tag="IWM23_002728"
     CDS             4980..5852
                     /locus_tag="IWM23_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAP9885314.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT         1945 a         1049 c         1077 g         2037 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt ccaaaatcaa ataatataga ttaatcaaat atttgagaat aacattttat
     4801 ttaatctttt catatatcaa ttgtatgaaa aattattaga attttaaaaa atgataaaaa
     4861 ttcaaaaaga aaaaatagta gttatttact tgtcatgtta ctaaataaaa aaataattta
     4921 aatatgatat atagtcattt atttagtgat ttcattctaa aataaaggga gaaatgtcta
     4981 tgctatacgg tgcaattgaa gcaggcggca caaaatttgt gtgtgccatt ggcgatgaag
     5041 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatgcctt
     5101 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     5161 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     5221 aattggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     5281 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     5341 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     5401 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     5461 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     5521 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     5581 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     5641 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     5701 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     5761 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     5821 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     5881 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     5941 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aaattcttgc tcgaaagtta
     6001 tggaaatttc gatagccgta ggccacttgt attgtgcaat agttttttac acgccgtttg
     6061 tataattaag cgagacaaat aaaaaagcca tttatgttac actcaaat
//
LOCUS       DACDXE010000118         6107 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.120, whole genome shotgun sequence.
ACCESSION   DACDXE010000118 DACDXE010000000
VERSION     DACDXE010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.120"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            125..1099
                     /locus_tag="IWM23_002729"
     CDS             125..1099
                     /locus_tag="IWM23_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9885315.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1081..1542
                     /locus_tag="IWM23_002730"
     CDS             1081..1542
                     /locus_tag="IWM23_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAP9885316.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1592..2056
                     /locus_tag="IWM23_002731"
     CDS             1592..2056
                     /locus_tag="IWM23_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAP9885317.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2070..2819
                     /locus_tag="IWM23_002732"
     CDS             2070..2819
                     /locus_tag="IWM23_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAP9885318.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2819..3649
                     /locus_tag="IWM23_002733"
     CDS             2819..3649
                     /locus_tag="IWM23_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAP9885319.1"
                     /translation="MENSTEKKRLITKKDLNKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTSFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3649..4296
                     /locus_tag="IWM23_002734"
     CDS             3649..4296
                     /locus_tag="IWM23_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9885320.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4394..5170)
                     /locus_tag="IWM23_002735"
     CDS             complement(4394..5170)
                     /locus_tag="IWM23_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9885321.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLAESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5314..>6107
                     /locus_tag="IWM23_002736"
     CDS             5314..>6107
                     /locus_tag="IWM23_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885322.1"
                     /translation="MTYTHLTTDELVIIESYFNIEKPVALVAQSLNRSRQTIYNVYSF
                     LKQGKKVLDYYRQYKQNKSRCGRRSTVLSDEQSTYVQEKVAQGWSPDVIIGRGEFPID
                     CSVRTLYRMFKKGLFDTQSLPMKGKRKPNNHQEKRGKQAFRRTIHERNSLYPHLTKEF
                     GHLEGDTIVGVKHKSAVITLVECLSKVIITLKPTGRRAEDIENRLNDWFKSMPKHLFK
                     SMTFDCGKEFSNWRSISNSNDIAIFFADPGTPSQRGLNEHSNGLLRR"
BASE COUNT         2083 a          882 c         1111 g         2031 t
ORIGIN      
        1 atataaaatt gttataaaaa aatttttttg aaaaatagta ttgcaaaata aagcaatttc
       61 atagataata ggtattgtaa agttatatga ctttataata cttattagag tgagggataa
      121 agaaatgaga caactacaca aagaacagat tgaaacagca atggcagcgt taaaggaaaa
      181 gacaattgat catgtatatt ttacagcatg tgggggttca caagccgttt taatggctgg
      241 tcaatatcta tttgataaag aattaactgt accttgtcat gtttatactg ctaacgaatt
      301 taactatgct ataccaaaag gattatctga aaattcttta gttatcactt gttcccattc
      361 tggcacaaca ccagaaactg tagaagcaac aaaaaaagca agagaatttg gagcaatgac
      421 tatttgttta tctaatgaag aaggatctcc tctatgggaa gcagctgaat atcctgttca
      481 ctatgattgg ggtaaagatg ttgatgctag tgatttaaat aaaggaattc tttatggttt
      541 attatttaat attttacttg ttttagatgg ctctaaaaaa tgggaaactt gcttgaatga
      601 atttgataag ttaaatgatc ttgttattgc tgaaaagagc aaatttgaaa taactgctaa
      661 agaatggggt aaaaaccaaa aacgagataa aattatttac acaattggta gcggtatcaa
      721 ttacggagaa atttactcca cagcagcttg ctggttcatg gaaatgcaat ggattaattc
      781 taatgctatt cattctgggg aatttttcca tggtcctttt gaaatcactg attatgatgt
      841 accattcatc ttagtgaaaa gtattggaga tacaagattt ttagatcaac gggtagaaga
      901 cttcactaaa aaattcagta ataaattaat ggttttagac caagctgatt ttgacttaga
      961 atctatagat agtgtagcga aagaatatat tgcagctgta cttagtggcg ttgttattcg
     1021 ccaaatggta gaagcgattg cttttgaaag aggccattcc ttagatgtta gacgctatat
     1081 gtggcaaatg aaatattaat taataggagg ttgcaaaaga tggcaaatca attatttcac
     1141 attgtgatta caacacattc taccctttgt gagggttatt taaaggcaac aaaattaatt
     1201 ttaatgaatg acgccacagg tattagtgct gttccattta aagaaaatac tagtccagat
     1261 gattttgagg agagtatgaa agaaataatt agtcagcatc atttgcaacc tctaattatt
     1321 ttaacggatt taattggagg aacacctaat aatgtttcta ttaaacagtt gaacaacaat
     1381 agaattcaaa ttattgctgg gattaattta cctcttttac tggagttatt aatggcccag
     1441 tcagctggta aatctataga agaaatagat tttaaggaat tactgaataa ctctcaaaaa
     1501 agtatggttc atattaattc gcttttaaaa gatgtcagtt aaggtggcaa tttctattct
     1561 ataaatttta aaaattgaag gaggaaatag aatgattaaa ttagtgcgtg tagaccacag
     1621 attacttcat ggacaagtaa tattttcttg gactaaacag tatgatatta accatattat
     1681 tgtggctgat aacaaagttc caggagatcc aatgtctgtg atggctttga caattgctaa
     1741 acctgctgat tgcaaattag acattgttaa aattagtgat gttaaaggga ttatggaaac
     1801 atccccaaat gataattaca tgattttaat taaaggacct aaggaagctt ttgaattagt
     1861 tcaaaattta ccagaaatta aagaaattaa ttttggcggt gtgtctaaga aaagcgagtc
     1921 caaacaatat ggaaaggcag tctttttaac tcctgaagaa ttagaagcca caaaaaaaat
     1981 tcttgatcaa gaagtgaaaa tatttgtgca acaagtccca tcttcaagta ttgaaaatgt
     2041 agattttagt aagtaagggg ggaaaataaa tgttattaaa agccatttta ttaggtttag
     2101 ttggtgtggt agctgttatt gactccaggt taattggtcg acaaaatatt ggtagaccac
     2161 taattttgag tacacttgcc ggacttgtgc taggtgactt gcatacaggg attgttcttg
     2221 gggcatcttt agaattaatt tctatgggat tcgtctcaat tggggcggct ggacctccta
     2281 atatgcaact tggtagtatt attgccactg cttttgcaat tttaactaat agtagtacag
     2341 aaacagcctt aacaatagct atcccagtgg ctgtagctgg tgaatttatg agtattatca
     2401 tgcgcatgat aattgctcaa tttggacatg ctgcagataa agccattgaa aataataaat
     2461 ttagaaaagc tcaaattatt catatttatt ggtcatttat ttttaatgct tttgtttact
     2521 ttattccaat ctttttaaca gtatattttg gtgctgacgt tgtggcagat ttagtagata
     2581 aaattccaga aatgattaca gacgccttga ctgtctctgg aaatatgctt tccgcattag
     2641 ggtttgcaat gctattaagc acaatgttga gtaaaaaatt atatccttac tttatttttg
     2701 gattctttat tgttgcctat tcaggtcttt cactaattgg cgtgacaatt tttgcagctc
     2761 tgattgcctt tgtaatggat caagttaaat atggaaaaag agaagaggcg catggttaat
     2821 ggaaaatagt acagaaaaaa aacgtttaat cacaaaaaaa gatcttaata agatgtgtct
     2881 tcgttcatgg acaatcatgt gttcgtggaa ttatgaaaga caaatgcata tgggatttat
     2941 gtatgggatg gcgccaattt taaataaagt ttataaaaat gatgaagaga aaaaaagaga
     3001 agcttatact cgtcatatgg cattttttaa ctgtacgcct caaatgactt catttattat
     3061 gggactttgt gcttctatgg aagaacaaaa tgctcaagca gaagaaggaa actttaatac
     3121 tgaatctatc tccatgatta aaaccagttt aatgggtcct tttgctggaa ttggtgattc
     3181 atttttccaa ggtactttaa gaattattac atttgggatt ggattgtctt ttgctaagca
     3241 aggtagtatt ttaggaccga ttttagctgt tggccttttt gctatacctt ctattttagc
     3301 tgttttttac ggcggtaaat taggatatga gtctggcaat aaatacctta ctaaattgta
     3361 tcaagaaggt cttatggatc gagtaatgca atttgcttct atcattggtt tggctgtagt
     3421 aggtggaatg gtggctagca tggtgccaat tgtcacccct ttaaaattca ctacaggggg
     3481 aacagaactt gttttacaag acttacttga ttccattatt cctggattat taccacttgt
     3541 atgtactttg ggaatatata aattgattaa gaaaaatgtt agtacaaata tgctcttatt
     3601 aggttgtatt ttggcagggt ttgttttaag tataataggt atcttataat gattaaggct
     3661 ctaatttttg atatggacgg agttttaatt gatagcgaga ttatgtatca aaagtgggta
     3721 tttaattttt taaaacaaga aaatttaagc tatcctttgt attcttatca aaaaaagatt
     3781 ggaactacag caagtatttt tgatgatata gaaaagtaca atgagggatg taacagttta
     3841 gaattgaagg gaaaatttac taaatattgg aaatcgcaaa agattgatta cttggatatt
     3901 tttcctaata tactaattga acagcttgaa tggttaaaaa agaaaaaaat aaaaatggca
     3961 attgcttctt ccagtccaat ggatactata aatgaagttg ttaccacttg taaaatagag
     4021 tcatattttg attgtttaat tagcggaaga gatttgccag aaagtaaacc taatcctact
     4081 atattcctta aagcttcaga acagctcaga attccagttg aagaatgtct ggtgatagaa
     4141 gattcttata atggtataaa agctggtaaa agagcaaata tgaaggttct tgcaattaaa
     4201 gataaacgtt tttctcaaga tgtaagtcta gcagatgatg ttatttacga tattaaatat
     4261 ctgcgacaag aagtacaggt gaaatttgga atataaataa gttttttgat gtatttagag
     4321 aatattagta aaaataaaaa tcatgcccaa atctaatcaa aattgattgc tttgggtatg
     4381 atttctttta atatcaatct attactttag gatctgaatc atttttatgc accgtatcta
     4441 aagtgatatt ttctgtgact tcaataacat aactaattct atcacaacga actgtggatt
     4501 gagaataatg aaatggttga ttatttttat ctttgccaac cttttctaaa ataaatagcg
     4561 gatctcctac tatacattgc aataagtcgg aaagctcagg tgttgcagat aaaccgttta
     4621 tttccataat agagctcgta gctttgacat catagtcatc ttctagaagt ttatataatg
     4681 aagtatattg gctacctttt tcaaataaag ttggaaattt ttcttctgat gcgaaaaaat
     4741 tatcaattcc catcggggca ccgtcttccc acattaaacg tttaatattg taaactctat
     4801 cagttacatt gatttgtaat ttattagcta aaaactcatc agcctttata attttagaat
     4861 gtaaaacagt atatctaatt ttatgtcctc gggtagaaag agaatcgcta aatccatgaa
     4921 ctccagctaa ttttaaactt tctttttttt gagttacaaa ggtccctttc ccccttaatc
     4981 gttcaactgt tccactttct gctaaaagtt taaaagcttt tcgaatggta atcttactta
     5041 ctccataaat tttacataat tcttcttctg taggtaattg ctctcctgga agatattttt
     5101 tttcacgaat atcgttacgt atagaagctt ctacttgtaa atataatggt ctattatctt
     5161 gaaaattcat agtacactcc ctaaaaagtt atacttcatt atatcacaat ttattaaaac
     5221 gtcgattgta aaattaagct agacaactaa aaagatcatt tgtgctacac tcaaaatagt
     5281 tccgaccaaa gaattaataa ggagtgagta caaatgactt acacccatct tacaacagac
     5341 gaactagtaa tcatagagtc ttatttcaat atcgagaaac ctgtcgctct tgtcgcacaa
     5401 agtttaaatc gctctagaca aaccatttat aatgtttatt cgtttctcaa acaaggaaaa
     5461 aaagtgctag attattaccg tcaatacaaa caaaataaat ctcgttgtgg acgacgttca
     5521 accgttttat ctgatgaaca atcaacatat gttcaagaaa aggtagctca aggatggtca
     5581 cccgatgtga ttatcggccg tggcgagttt ccaattgatt gttctgttcg tacactctat
     5641 cggatgttta aaaaaggcct ttttgacaca cagtcgttac ctatgaaggg gaaacgtaaa
     5701 ccgaataatc atcaagaaaa gcgtggaaaa caggcctttc gcagaacgat tcatgaacgg
     5761 aacagtctct atccacacct gactaaagaa tttggtcatc tcgaaggcga caccattgtg
     5821 ggagttaaac acaagagcgc tgttattaca ttggttgagt gtttatccaa ggttattatt
     5881 actttgaaac cgacaggcag acgagccgaa gatatcgaaa acaggttaaa tgattggttc
     5941 aaatccatgc ctaaacactt gtttaaatcc atgacatttg attgtggaaa agaattttcc
     6001 aactggcgat ctataagtaa ctcgaacgat attgccatct ttttcgcaga tccaggaaca
     6061 ccttcgcaaa gagggctaaa tgagcactca aacggcttgc ttagacg
//
LOCUS       DACDXE010000119         6001 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.121, whole genome shotgun sequence.
ACCESSION   DACDXE010000119 DACDXE010000000
VERSION     DACDXE010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.121"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWM23_002737"
     CDS             <1..395
                     /locus_tag="IWM23_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885323.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="IWM23_002738"
     CDS             398..1228
                     /locus_tag="IWM23_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9885324.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="IWM23_002739"
     CDS             1244..2095
                     /locus_tag="IWM23_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAP9885325.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="IWM23_002740"
     CDS             2092..2451
                     /locus_tag="IWM23_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HAP9885326.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="IWM23_002741"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="IWM23_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="IWM23_002742"
     CDS             2624..2929
                     /locus_tag="IWM23_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885327.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="IWM23_002743"
     CDS             2929..3240
                     /locus_tag="IWM23_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885328.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3407..3823
                     /locus_tag="IWM23_002744"
     CDS             3407..3823
                     /locus_tag="IWM23_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAP9885329.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="IWM23_002745"
     CDS             3823..4491
                     /locus_tag="IWM23_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAP9885330.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="IWM23_002746"
     CDS             4488..4865
                     /locus_tag="IWM23_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885331.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="IWM23_002747"
     CDS             4862..5161
                     /locus_tag="IWM23_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885332.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5403..5819
                     /locus_tag="IWM23_002748"
     CDS             5403..5819
                     /locus_tag="IWM23_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAP9885333.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2230 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 g
//
LOCUS       DACDXE010000120         5976 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.122, whole genome shotgun sequence.
ACCESSION   DACDXE010000120 DACDXE010000000
VERSION     DACDXE010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.122"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..382
                     /locus_tag="IWM23_002749"
     CDS             <1..382
                     /locus_tag="IWM23_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HAP9885334.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="IWM23_002750"
     CDS             complement(776..1783)
                     /locus_tag="IWM23_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAP9885335.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="IWM23_002751"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="IWM23_002751"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAP9885336.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="IWM23_002752"
     CDS             3679..4002
                     /locus_tag="IWM23_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAP9885337.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="IWM23_002753"
     CDS             4013..5695
                     /locus_tag="IWM23_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAP9885338.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
BASE COUNT         1896 a          898 c         1213 g         1969 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctc
//
LOCUS       DACDXE010000121         5925 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.123, whole genome shotgun sequence.
ACCESSION   DACDXE010000121 DACDXE010000000
VERSION     DACDXE010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.123"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..1170)
                     /locus_tag="IWM23_002754"
     CDS             complement(76..1170)
                     /locus_tag="IWM23_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9885339.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="IWM23_002755"
     CDS             1359..2120
                     /locus_tag="IWM23_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAP9885340.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWM23_002756"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="IWM23_002756"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAP9885341.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2895..3650
                     /gene="fabI"
                     /locus_tag="IWM23_002757"
     CDS             2895..3650
                     /gene="fabI"
                     /locus_tag="IWM23_002757"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAP9885342.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3760..4773)
                     /locus_tag="IWM23_002758"
     CDS             complement(3760..4773)
                     /locus_tag="IWM23_002758"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAP9885343.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4938..5858
                     /locus_tag="IWM23_002759"
     CDS             4938..5858
                     /locus_tag="IWM23_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAP9885344.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1806 a         1181 c         1063 g         1875 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgacaatc aaaacatttt gactaataat taagaaatca
     2821 agaagttatt ttagctatta ttctctattt ttttacgact atcaaatttt tatcagaaac
     2881 aaaggagagt cattatgtca tttctagaag gaaaaaaaat cgtcattatg ggcgtcgcta
     2941 acaaaaaaag cattgcttgg ggttgtgctg aagcaatgtt gaaacaaggg gcagaaatta
     3001 tctatactta ccaaaatgaa cgaatgaaaa aatcattagt caaattggtc ggcgaagaag
     3061 cattcactgt agaatgtgat gtttctagcg acgaaagcat cgaaaaagca atgaatgcca
     3121 tcggtgaata tgctggggag atccacggcc ttgtccatgc agttgcttac gccaacaaag
     3181 aagaattgtc cggaaatgtg tctgatattt ccagagacgg ctatcaatta gctcaagata
     3241 tcagcagcta ctctttaatt gctgtatcaa aatacgcaca accgtttctt gcaccaaaca
     3301 gcgggatcgt tagcatgact tatctaggtt cagaacgcgc aatcccgaat tataacatga
     3361 tgggtattgc gaaagcttct cttgaagctg ctatccgtta tttggcagct gagttctcgc
     3421 ctaaaggaat tcgggtcaat ggaatctcag ctggggcaat caaaacatta gctgtcacag
     3481 gtgttaaaga ctatcaaaaa ctgatccagt tatctgaatc tcgtacgcct gatcagcaag
     3541 gtgtgacgat cgaagaagtc ggtaatgctt gtgctttctt aatcagccca ctttccagcg
     3601 gaatcatcgg tgatattatc tacgttgaca aaggtgtcca tctttcataa acttttgttg
     3661 cattctttaa agtaaaacat ataaaaatca ggaaaaaact ttttaaagtc tcttcctgat
     3721 tttttctttt tacgtttttt tcaactcatt actttgaaat tattccacga cttcaatcca
     3781 gccttctggt gcttctatgt ctccaaattg aatacctgtc aattcatcat acaaccgttt
     3841 tgttactggt cctacttccg tttcactata aaatacatga agattgttat tgtgataaat
     3901 cccaccgatt ggagaaataa cagccgctgt accacatgca ccagcttctg caaattgatc
     3961 tagatcatca atgaaaatat ctccttcaat tgcttgcagt cctagtcgtt cttcagctaa
     4021 ctgcaacaag gaatatttcg tgatacttgg taagatagag ggcgattgcg gagtgatgaa
     4081 ttttccgtct tttgtaattc caaagaaatt tgctgcacct acttcttcga ttttcgtatg
     4141 tgtggctgga tcaagataaa cacaatctga gtaatccagt tcttttgctt ctgttcctgg
     4201 aagtaaactc gctgcgtaat ttccgccgac ttttgctgca ccagtcccct taggagcagc
     4261 acgatcgtaa tcagatacaa taaaattcgt tggttttagt ccacctttga aatacgctcc
     4321 tactggcgta cagaagacga caaataaata ttctggagct ggatggacac caatgttttc
     4381 accgacccca atcagtaaag ggcgcaagta taatgtagcg cctgttccat agggcggcac
     4441 aaaatcttca tttgctttta ccacttgctt gacagcatca acgaacatct tttcagggac
     4501 cattggcatc agcaatcgtc tagcactttg attcatccgt cgactatttt gttcaggtcg
     4561 aaagagattg accttgccgt cttttcgacg ataagctttt agaccttcaa aacattgctg
     4621 accataatga agcgctggag agccttcatg aatcgtcagg taattatctt ctgtcaattc
     4681 tccttcttcc cattgtccgt ctttccattt ggcaataaaa cgccaaggag ttttgatata
     4741 agaaaaacct agattgttcc aatcgatttg catatactta ccaccttcgt tattttctcc
     4801 acattttatc attatccata ttatttgtca ttttattttc agcttttttc cttcattata
     4861 gttatttttg ctatttcctt ttttgaaaac ttcttggtac aatacagaaa gaatcaaatt
     4921 ttagaaaaga ggattttatg ttttcattat tagcaactgc cggtacgaca gactcttctg
     4981 gcgagacatt gacagatcaa gctgtcagca aagtaaatgc gtttcagcgg ttttgggacg
     5041 ggattgattg ggacgccatt ttggctgcct ttatccaaaa aagtttatat ctgatttttt
     5101 tgatcgtcct tttttggatt cttcaccgtg tgggaaaata tctgattgat aagtctttta
     5161 atcagtattc taaaaaagcc attctaaacg agagccgctt aaagacttta catagtcttt
     5221 taaatactat ttttcattat acccttggtt tcttttttat ctatgcaatt ttatctgcta
     5281 tcggtgtgcc gatcggatca cttttggctg gtgccggaat tgcaggtgtg gcaatcggtc
     5341 ttggtgccca agggttcatg agtgatgtta ttaccggatt ttttattatc atggaacaac
     5401 agatggatgt tggggactac atcaagctag caaacttaac gattgaagga accgtcgtat
     5461 ctgttggtat ccgtaccctt cagctgaaag caacggatgg tactgtccat ttcatcccga
     5521 atcgaaacat caccacgatc agcaatctct ctcgtgcaaa tatgcaagta cttttagata
     5581 ttcggattgt tcctgaagaa ggatatgaca aaatctatga gatcattgat cgagtaaatc
     5641 gtacattagc tgaaaaatac caagaagagc tccaaactga accttctatc tttggattag
     5701 tggatcttgg aaacagcaat tttgcgattc gtacggtctg ctacgcattg aacggaaaac
     5761 aatatgcctt aaaagaagag ttcttgagct cctatgtcaa agaattgaca gcttctggtt
     5821 tcacgattcc gaacagtcca attgctgtcg gaaaataatt tggacaaacg atgcaacgaa
     5881 tctcactaaa aagagcgtgg gacaaaagta aagaacactt ttgtc
//
LOCUS       DACDXE010000122         5829 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.124, whole genome shotgun sequence.
ACCESSION   DACDXE010000122 DACDXE010000000
VERSION     DACDXE010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.124"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1253)
                     /locus_tag="IWM23_002760"
     rRNA            complement(<1..1253)
                     /locus_tag="IWM23_002760"
                     /product="16S ribosomal RNA"
     gene            1173..1316
                     /locus_tag="IWM23_002761"
     CDS             1173..1316
                     /locus_tag="IWM23_002761"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885345.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(1939..3444)
                     /locus_tag="IWM23_002762"
     CDS             complement(1939..3444)
                     /locus_tag="IWM23_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9885346.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(3441..4127)
                     /locus_tag="IWM23_002763"
     CDS             complement(3441..4127)
                     /locus_tag="IWM23_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9885347.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IWM23_002764"
     CDS             complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IWM23_002764"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAP9885348.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1543 a         1325 c         1037 g         1924 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat attgcttgct tgttaaaaat
     1321 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
     1381 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
     1441 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
     1501 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
     1561 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
     1621 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
     1681 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
     1741 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
     1801 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
     1861 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
     1921 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
     1981 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
     2041 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
     2101 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
     2161 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
     2221 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
     2281 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     2341 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     2401 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     2461 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     2521 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     2581 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     2641 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     2701 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     2761 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     2821 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     2881 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     2941 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     3001 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     3061 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     3121 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     3181 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     3241 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     3301 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     3361 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     3421 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     3481 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     3541 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     3601 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     3661 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     3721 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     3781 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     3841 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     3901 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     3961 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     4021 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     4081 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     4141 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     4201 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     4261 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     4321 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     4381 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     4441 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     4501 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     4561 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     4621 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     4681 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     4741 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     4801 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     4861 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     4921 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     4981 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     5041 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     5101 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     5161 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     5221 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     5281 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     5341 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     5401 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     5461 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     5521 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     5581 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     5641 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     5701 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     5761 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     5821 caacatatt
//
LOCUS       DACDXE010000123         5716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.126, whole genome shotgun sequence.
ACCESSION   DACDXE010000123 DACDXE010000000
VERSION     DACDXE010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.126"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(138..428)
                     /locus_tag="IWM23_002765"
     CDS             complement(138..428)
                     /locus_tag="IWM23_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885349.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(546..806)
                     /locus_tag="IWM23_002766"
     CDS             complement(546..806)
                     /locus_tag="IWM23_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885350.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(943..1356)
                     /locus_tag="IWM23_002767"
     CDS             complement(943..1356)
                     /locus_tag="IWM23_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAP9885351.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="IWM23_002768"
     CDS             complement(1812..2297)
                     /locus_tag="IWM23_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885352.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="IWM23_002769"
     CDS             complement(2436..2651)
                     /locus_tag="IWM23_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAP9885353.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="IWM23_002770"
     CDS             2875..3675
                     /locus_tag="IWM23_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAP9885354.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="IWM23_002771"
     CDS             3694..5562
                     /locus_tag="IWM23_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAP9885355.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..>5716
                     /locus_tag="IWM23_002772"
     CDS             5555..>5716
                     /locus_tag="IWM23_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885356.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSG"
BASE COUNT         2108 a          895 c         1018 g         1695 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggc
//
LOCUS       DACDXE010000124         5689 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.127, whole genome shotgun sequence.
ACCESSION   DACDXE010000124 DACDXE010000000
VERSION     DACDXE010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5689
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.127"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(129..800)
                     /locus_tag="IWM23_002773"
     CDS             complement(129..800)
                     /locus_tag="IWM23_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885357.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(793..1287)
                     /locus_tag="IWM23_002774"
     CDS             complement(793..1287)
                     /locus_tag="IWM23_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAP9885358.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(1307..2527)
                     /locus_tag="IWM23_002775"
     CDS             complement(1307..2527)
                     /locus_tag="IWM23_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885359.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            2674..4509
                     /gene="lepA"
                     /locus_tag="IWM23_002776"
     CDS             2674..4509
                     /gene="lepA"
                     /locus_tag="IWM23_002776"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAP9885360.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(4603..5685)
                     /locus_tag="IWM23_002777"
     CDS             complement(4603..5685)
                     /locus_tag="IWM23_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9885361.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         1739 a         1144 c         1083 g         1723 t
ORIGIN      
        1 aaaaaagggg tctgacacaa ctagtgtcag acctcctttt ttcaaataaa cagtgttcca
       61 gaagtagttt cttcgaaaaa taagccgaaa tttcttgaag ctgcccgctt ctgtcacaac
      121 ctcttttatt aggtattttt cactttttct aatttataac tttcaaagta acgctcatct
      181 tttttacttt gagaccatgc aaacaatccc attaaggcag cacctatcgt atttgtcagc
      241 aagtcgccca tcgtatccat caatgctgct cgtccgacaa acagtgtgcc atcagaagca
      301 gcaaaacgtt gaagattcat gcctaagaac tggtcgcata agaattccca gaactcccaa
      361 aacacgccgc aaagtccagc aaacgcaaag ccgaataaga ggaacaacca aggactggtt
      421 tttgatatct cagcatcttt catcaagtaa ccaatcaatc catagccaag agctgtcagg
      481 accattggac tcaccgcatg taagattttg tcccaaaaag agatgatact gatcaaatgc
      541 atccctgtgc ctaaaaagac agagatgaac aagaaaaacc agtaaaacaa aacgattgct
      601 gctggtattt taagtttgaa aactttcaaa atgacttgtg gcatgaaaat caatgcaatc
      661 cctaataaag attccacgat aaatgtgatt ccttgagaag tcgaatattt ttgtgtgata
      721 aattcataaa aattatagca gacaacgaat acaccaaaag caagtaaagc tatttgcacc
      781 tttttgattt ctttaatcac tgaaagtccc tccatctaaa aatcttttta cttctgaaag
      841 caactcaatt tctgaagaaa ccacttttcc gttcaatttg atcaaaccta ttgtatataa
      901 gttcaggtag tgaaattgat tttcagctgt ttcttgtagt gcagcgattt ttttcgggtg
      961 ggttccgcct tgctgtcgag tatcagtata taagccgatc actgggatgc ctttcgcata
     1021 agccacaccg atttccgatg ctaccccggc atcgatcgtc agcccatcta gtaaagcaat
     1081 cattaaatca ctagccaaca ctttttctgt gtcagcaaga gcaatcattc gactgtctgc
     1141 ataagcagtt ttgtcattga ttgcttcatt ttcttgagga agatagatcg aaagctcaga
     1201 agagagctct ctgatttctt taactaactg ttggttatat aacaaatcac ttttagcaaa
     1261 cattggtgcg gcaaaataaa ttttcatcta aacatcatcc tttttatcaa aaataatcgc
     1321 taaagtaagc ttccatgttt ggaatgatac gctctagtga cttcagtgtt tcttcatctg
     1381 tttctaaccg ttcgattctt gccaaataag aagcacggcg ataattcatc atcaagcaag
     1441 tcaatgtttg gatatccagt cgaacggatt ttcctatagg ttcatttaag acacggactt
     1501 gtccctgttc atcccatgtt aaagcgaaga caccattatt ccaaggagcg acaggatctt
     1561 caatgatgaa atgaaaagca tcagctgttc caacaaacgg aaaccgttga aggaattctt
     1621 ttacatcaac gatccgagcc ataaaatatg gttcgatctg ctctttgatc tgactatctt
     1681 caatcaaaaa tgctaaaggc tcatttttat aaatatctcc atgtacccag tagatcatgg
     1741 aaaaatgtgc ggaaataaag ttccataagc catttcgagc ttcttgatca agatagaaca
     1801 tttcttttac gtgaaatatt tcttcagcta cccaatagaa cagaacacct cttggttcat
     1861 ggttgctatc gtagtagaca gcggctgtgc gctcatcttc attttcgaaa cgccaatact
     1921 cttcccaatg aaaatcactg cggaataaag caccatggtt ttgttgggca aatttgtcat
     1981 aaacggaaaa tacgtctgga tgatcaactg gtaatcgctc aaccatacca cttactggga
     2041 tttgtttagg tagctgtgta tctctgattt taaaggaaag tttatcagac atgatttccc
     2101 atcctttacg ccgatagtag gggatactgt aaggatacaa ataagagatc cattgcttat
     2161 tcttgcgcat attatccaat gcaacgataa ttagatcttg catcaatcca tggttagcat
     2221 attctggata agttccaact ccagtcacac cacccatttt ataacgtgca ccgtgaatat
     2281 tgacttcaca aggatagata gcgatttgag agatcagttt attttcgtgg aaccatccga
     2341 acactttcga tacttccaaa atcggttttt tggaacggat aaaagctcgt ttattctcaa
     2401 acccgctttc ctcgatgtct gcttcagtca cttggaacac ataggaaagc aattcattga
     2461 attgatcgat atgttcttga gtgaccggtt ttaattcaag attttctcta aaatcttttt
     2521 gatccatttt ttcatctcca tttattagct tgataacagt atagcaaaat tctacaaaaa
     2581 aaacttctat agctgactaa ttaaaaactc tttaaattac tgcttttcgt tggtataatg
     2641 gaaagtgctg attatatgaa aagaggagac gctatggata taaagaaaat gaaagaacgc
     2701 caagagcaga ttcgtaattt ttcgatcatc gctcatatcg accatgggaa atctacattg
     2761 gccgatcgta ttttagaaat gacgcacact gtgacgtctc gcgaaatgca agatcagctg
     2821 ttagactcga tggatttgga acgtgaacgt ggaatcacga ttaaattaaa tgctgtagaa
     2881 ctcaattaca cagcgaaaaa tggagaaaca tatatctttc acttgattga caccccaggg
     2941 cacgtcgact tcacctatga agtctctcga agccttgctg cctgtgaggg tgccgtttta
     3001 gtggtcgatg cagcacaagg aatcgaagca caaacattgg ccaatgttta tttagcgtta
     3061 gacaatgatt tggagatcct gccagtcatc aataaaatcg acctacccgc agcagatccc
     3121 gaacgtgtcc gtcaagaaat cgaagacgta atcggaatcg atgctagtga agctgtatta
     3181 gccagtgcca aatcagggat tgggattgaa gatattctcg agcagatcgt ggaatatgtt
     3241 cctgcaccaa gtggagactt agatgcaccg ctaaaagctt tgatctttga ctctatttat
     3301 gatagttacc gcggtgttgt tctaaatgtc cggatcacgg acggtatggt caaacccgga
     3361 gacaaaatca aactgatgag taatggcaaa acatttgatg ttacagaggt cggggtcttc
     3421 tcgccaaaag cagttgctag agactttttg atggtcggtg atgtcggtta tatcacagca
     3481 agtatcaaaa ctgtccaaga tacacgagta ggggatacag taacgttagc agacaatcct
     3541 gccgctgaag ctttaccagg ttaccgaaaa atgaatccga tggtttattg tggtctttat
     3601 ccaattgata cttctcgtta caacgatttg cgggaagctt tagaaaaact gcaattgaat
     3661 gatgctgctt tacaattcga accagaaacc tctcaagcat taggttttgg gttccgctgt
     3721 ggattcctcg gacttcttca tatggatgtc gtccaagaac gcttggaacg ggaattcaat
     3781 ctagaattga tcacgactgc accatccgtt atttaccatg ttaataaaac ggatggtacg
     3841 actgctacag tagataaccc ggcagatttt cctgaacctg taacgattca ggatgtagaa
     3901 gaaccattcg tcaaagcaca gatcatggtc ccaaatgatt ttgtcggtgc agtaatggaa
     3961 ctttctcaac gaaaacgggg agaattcatt acgatggatt atttagatga ttaccgagta
     4021 aatgtcgtct ataatattcc gctttctgaa attgtctttg actttttcga caaattgaaa
     4081 tcaagcacga aaggctatgc ctcattggat tatgaaatgt ctggttatca aaaaagcaag
     4141 ttagtgaaga tggacatctt gctgaatgga gaaaaagtcg atgcattaag ctttatcgtt
     4201 caccgagact ttgcctatga acgtggaaaa gcaatcgttg aaaaactgaa aaaactaatc
     4261 ccacgccaac aatttgaggt gccaatccaa gcagctattg gacaaaaaat cgttgcccgt
     4321 tcagacatca aagccttgcg taaaaacgtc ttggctaaat gttatggtgg agatgtttct
     4381 cgtaaacgta aactcttaga gaaacaaaaa gaagggaaga agcggatgaa acaaatcggc
     4441 tccgtcgaag ttccgcaaga agccttcatg gccgttctga aaatggacga ggacgaaccg
     4501 aagaaatagc aagggtttgt ttgaaaacag tgtaaaaaat aaaccatgtt tacatggatt
     4561 tgccaccggt ttgccaccgg tggttttttt cggtggcaaa aatcaagaat tctttctaag
     4621 attttccatg atatcgaccg tttcatttct catcttatct gttacatgcg agtaagtatc
     4681 catcgtaatt gaaattctgc tgtggcccaa tcgttcagag atttctttca tttttgcgcc
     4741 attttcgaga agaagtgtag catgagtatg tctgagagaa tggaagttaa aagagaggga
     4801 gagtgcattc gatatcctcc tagtattcca tttcactaca cttggcgtaa ctagctcacc
     4861 gtcttccttc gtacatactg catttgaatc aatgtagagc tttccatact tcattcgatt
     4921 ttctaattgt tgtttcttat gttttttcag aattgctagc aaggtttgtc caataaaaat
     4981 cgttcgattg gaactgcttg tctttggcgt accatatacc catgcgccgt cattctttac
     5041 catttgtttc tctacagtaa ttgttccatt tgaaaaatcg acatcatccc acgtcagacc
     5101 acaaacttcg ccaacgcgca ttcccgtata aaatccaata ttcaaaggaa tatagaaagg
     5161 atgtccttca ggagtaattt ctagcatatg atcaaagtcc tcaagagaaa tgatttttag
     5221 atctttttta gtcgttggtc gtgcttcata ttttggtatt tttacataca gcataggatt
     5281 ttgcttgatt aatccccaag gataaaccgc catattcagc gcattcttaa ggacagagtg
     5341 agtaatagtc attgttttct tcgagtaacc ctttttaaat tcagcattga tgaaattttg
     5401 taaaagagca ggggagagat ccgtaagttt tttctttcct aaataaccgt ttatatgatt
     5461 tttgatggta aatcggtagt tttcataggt attgtatttt agatttagtt taacgtattc
     5521 ctccatccaa aaatcaaggt attgttttac tcgagtatcc gtacctaaaa agtattgtcc
     5581 tgtttcgtca atatctgata aaacttttcg taaagcagct tcggcctctg gtcgggtgtc
     5641 tccgccaact ttctccactt tttttcttga gccatcatca ttgatatct
//
LOCUS       DACDXE010000125         5572 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.128, whole genome shotgun sequence.
ACCESSION   DACDXE010000125 DACDXE010000000
VERSION     DACDXE010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.128"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(118..432)
                     /locus_tag="IWM23_002778"
     CDS             complement(118..432)
                     /locus_tag="IWM23_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAP9885362.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(446..706)
                     /locus_tag="IWM23_002779"
     CDS             complement(446..706)
                     /locus_tag="IWM23_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAP9885363.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            876..1190
                     /locus_tag="IWM23_002780"
     CDS             876..1190
                     /locus_tag="IWM23_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAP9885364.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            1192..2847
                     /locus_tag="IWM23_002781"
     CDS             1192..2847
                     /locus_tag="IWM23_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9885365.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            2930..5065
                     /locus_tag="IWM23_002782"
     CDS             2930..5065
                     /locus_tag="IWM23_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9885366.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            5240..>5572
                     /locus_tag="IWM23_002783"
     CDS             5240..>5572
                     /locus_tag="IWM23_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885367.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFE"
BASE COUNT         1890 a          963 c         1133 g         1586 t
ORIGIN      
        1 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
       61 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
      121 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
      181 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
      241 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
      301 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
      361 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
      421 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
      481 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
      541 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
      601 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
      661 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
      721 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
      781 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
      841 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
      901 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
      961 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     1021 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     1081 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     1141 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     1201 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     1261 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     1321 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     1381 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     1441 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     1501 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     1561 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     1621 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     1681 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     1741 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     1801 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     1861 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     1921 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     1981 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     2041 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     2101 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     2161 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     2221 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     2281 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     2341 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     2401 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     2461 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     2521 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     2581 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     2641 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     2701 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     2761 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     2821 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     2881 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     2941 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     3001 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     3061 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     3121 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     3181 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     3241 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     3301 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     3361 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     3421 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     3481 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     3541 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     3601 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     3661 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     3721 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     3781 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     3841 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     3901 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     3961 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     4021 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     4081 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     4141 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     4201 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     4261 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     4321 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     4381 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     4441 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     4501 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     4561 taattcttcc actacttttg ctgatattat gcgttgtttc gatgactacc atctgccgat
     4621 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     4681 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     4741 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     4801 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     4861 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     4921 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     4981 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     5041 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     5101 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     5161 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     5221 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     5281 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     5341 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     5401 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     5461 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     5521 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aa
//
LOCUS       DACDXE010000126         5249 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.129, whole genome shotgun sequence.
ACCESSION   DACDXE010000126 DACDXE010000000
VERSION     DACDXE010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.129"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1665)
                     /locus_tag="IWM23_002784"
     CDS             complement(<1..1665)
                     /locus_tag="IWM23_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAP9885368.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQN"
     gene            complement(1707..2765)
                     /locus_tag="IWM23_002785"
     CDS             complement(1707..2765)
                     /locus_tag="IWM23_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAP9885369.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(2884..3339)
                     /locus_tag="IWM23_002786"
     CDS             complement(2884..3339)
                     /locus_tag="IWM23_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885370.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(3923..4726)
                     /locus_tag="IWM23_002787"
     CDS             complement(3923..4726)
                     /locus_tag="IWM23_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9885371.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT         1550 a         1026 c          727 g         1946 t
ORIGIN      
        1 attttgacca tatactcttt ctttcctagg tggaagacgt aaatctatat ttaaatgttt
       61 tcgtactaat tcagcactct tcaacatttc attataagca atttccagaa gagtttgttc
      121 ggataaagcc caaaatggaa ctattcgtcc aaatcggtta ataacttcag gctttagacc
      181 ttcaccaatc aaggcttgtt tgatccctga tgcgactttt cggtattctc tttttagttc
      241 agtgctgtat tcattagaat tttccttgtt tgtaacacga gatagtgagc tatatcggtt
      301 taacgaatcg aacatctccg cacctgcatt tgttgtcgca aataaaattg tatttgacaa
      361 tgaaacggtg tcgtgaactt ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc
      421 ttcatctaaa attgataaaa aggcatcgag tgtttctaca ccagctttct catattcatc
      481 acacacaata atcgcagaag gatcatgcct tacatgagta cctataagtc gtctaaaacg
      541 cattgcatca tctttggttt tgtagtccgg catattaact aagagaactt tattttcatc
      601 gccataaagt cctcttgcca cggctttagc cattgcagtt ttaccgaccc ctgttgatcc
      661 tgtaaacaga gcagttgctt ttggttgctt tcgaccttta aaacgatcaa atccaagctt
      721 catacgttta aacaacattg ttatagtata aactgctaac ggttgtcctt ttatggactt
      781 taattcctct tgaagttttt ccatatcaaa atcagaatct agctgaatat tatacttacc
      841 aaatatttgc aatagacttt ctcgattata agcatttccg tctactcgat cttttgcaat
      901 caaatcctcc attacagcaa ttgatttagc cggttcggac aaatcctcac gataaagttt
      961 gtttgcttgt acgatttctt gaagtaattc atcaggaatc ctttgcgata atctaaaaga
     1021 agcctgagga tcatctttaa cagaggatgt atttagccat tgtctcaaaa tatcgacgac
     1081 aacatcgctt tttagttcat tcaattctaa cggttgtagc cgtctcccta aggcatcatc
     1141 tcttttgata tagttttcat attcttttct tgtagtagcg gtaatcattt gaagtgattc
     1201 atttgctaac ggttctttta gcgaatcacc cccaatttta ttgccactcc caaaagagct
     1261 aacaacttta tgaaactcgt caatgaagag gacaaattca ttatctttgt cctcagcttt
     1321 taaaattcgc tcatattctt tcaatcttgg aattaatgtt tctaaacgtg tttggaggac
     1381 atcatctccc ccaacacgca attgtccgat tgataattta tatacttgta ctttctttcc
     1441 atcttgtcgc tgtctttcag cccattcact tgcaagtgct gttttacctg ttccggcttc
     1501 acctaacaag atagcttgcg gtgtttccgg tctattcaag atcagtgaca actcatggag
     1561 ttcgtcatca cgtcccacta acttatgttt tggtggtttc attggtgtga gagcatgatt
     1621 aaattcggtt tgaatattct taaaaaactc ggtactattt tccatctgtt tgtgttcact
     1681 tcctctaaaa taagaataat ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc
     1741 ttcatgtctc caaaagaatc tttgttaaag tcagcatttt tgtattgatc aatcaatttt
     1801 tgtgtatcat tgattgcttt aatttcggtc tccattcgac tgtctagttg acgaatactt
     1861 tcttgcgaat ctttatcgtc aggattttgt tgtaaacgtt ccagatactc agcttttgtg
     1921 gcttcaattt tcttgatgtt agtatttgaa gcatttaact gatcttctag cttatttaca
     1981 acattattct tacttgtaac tgctacaact tttgaataat caatatcgcc atctttggta
     2041 agaaagcttc cttgatagac attagttgaa tctgaatatg gatttattct gaaattgata
     2101 ttatcaggag tattaacagg ttttgctttt ttcccaaaag aaattactcc ttcattatta
     2161 acatcattca tagaatattg agaaagtgct tttgtaactg aatctgcttc attcacattg
     2221 actttggtat catcgtcagc tgtatcatca ccagaaatta gtgatacttg gtttgcaata
     2281 aaaacttgat aagctctttc ctctaattta ccttttagga ataagaaagc atccccatca
     2341 gtacttaaca tcccatagct catttcaagc ttcttaggac gatctttttc ttttgttgcc
     2401 atatataagt tatagttttt tccttgcgta gataacaact ttctagcgtt ttgggagtaa
     2461 ccaagtttta ctactgttac atctctattt tgatctgtcc acacatctga aatagtgatt
     2521 tctgctcctg atcgagcaaa ctccaatgaa gaccctatag gtgttgtatt gttcacgaga
     2581 taataattat agtttcctaa ttgataaatg tagctaccca agcctgttaa taaaaaaagc
     2641 actcctagaa aagttgcaag cataatgcgt aatccgtgac gtgttctctt atccttatca
     2701 ccaagcaacc ctccaataat actgtattga tctttggtag atgttgaatt gacgtttttt
     2761 tccatcagtt tgtctgcccc ctttcataaa agagcaaaca atcagacgtt ttttcggtaa
     2821 ttctaggttc gggattttga atcatttcgg tatattcttc cccattaata tttaacacaa
     2881 aaattatctt cctttcttcg ttcctttctt ttgctcttta ttttccaaat aatcaatgat
     2941 ttttgctatt gtaccaagat agtaattccc ctcagctttg ttttttcgga ttttgctaac
     3001 cattggagta ggtacaatct cttcatctct caattcactg aaagattttt tacctagata
     3061 ttttccaata acagatttta aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc
     3121 ttttagttca tggttcatat tgtcaacgac ttttgcggtt tcaataattg ttttagttct
     3181 cagcttaata atttcatctg gaattttcaa tcctcgtaat ttcatcgttg tactaactgg
     3241 taacccatgc tttactgcat cagcaatact aaaacttttt agatcatcta gtaagctctc
     3301 catatagtta acaaattcat gccaattaat agcattcatt gtctcacccc ttcatttttt
     3361 taaaatgagg ttcgggcgta acacatttat gttattataa tatcataata acataaataa
     3421 atcaatcagc aaatttagat atcccttaaa gtatcagcaa ttattcaaca agatatttat
     3481 tttagctatt tcagtcatca taaaagacta attagcaata aaataactaa tattatccat
     3541 ttatcagacg aaaagaaaaa acttatctaa ttactctata ttaactttta acaaatagtt
     3601 gacttgtcca aaaatgttac cttcaatctc acaaagatat taagatgttt cctaccaaaa
     3661 ctgatatcac cggacttttt attctgtgtt gtccaaaagt gttaccttta gtcgatcaac
     3721 aaattaaaaa aaattgtttt tttcttgact tgtccaaaag tgttacccat agttgttaaa
     3781 atttgtatag attagccatt ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa
     3841 acttcttgca tttattgtcc aaaagtgtta cccatacttt tttaaagaag caggttgtat
     3901 taaaattaca acctgcttct tctcaatctt cttctaacca attatgcatt gaaatggttg
     3961 gcaagtttga ttctggctca ctaaaagtga atctgagccg atcaactctt cttcctttct
     4021 ttgcatagat tttttcataa tcaaaagttt caaatacctg tattccgttt tcatcaggag
     4081 ctaaaaattc ttctttcgcc tgctctagta ttcttttgtc aatatttgtc attctataac
     4141 ttgaaggtat acttaatgct cttctaaaat cttcaacttt gactacccaa taaccatttc
     4201 gccactcttt atttctgtat tgcatgagat gacgatacaa ttcttttgtg tagctacttc
     4261 ttagttttgt aaaattatca agttcaaaac gagtaaagtt tttgccaatt gagtttaaga
     4321 taaattcaaa ttcctcattt gccttaattg taactgtttc cttctgttta tctatccgga
     4381 atttttgaaa aagccacagc tgttcataga cgtctgaatc ttcaaaacgg taggttaaag
     4441 tacctagttt gtcactcaaa gcttttaaca cagaataaaa actgtccttt tctcttctgt
     4501 cgtatttgac caaataacga aagaggttga aatcaaaagt tacttctgct gttccttgac
     4561 gtttcatttt tgataaaata gcccaaaaca catccatttc aacaggctta aatcctcgca
     4621 aagctgttgt attcagacca ttgttataac gcacaattgc atttgtttgt tctgatacta
     4681 aatctacatc actatcaata atcttttcgg taacttcttg agacattgat tactctccaa
     4741 tcttcttttt gcgctccttt aatcatgtta atctggtact aattaaagga aacaattttt
     4801 tctgcttgtt acctaaatta aatcacaact aaaggtaaca gtcaacaaag atttgttact
     4861 aatctaattt tctatttttt cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc
     4921 tagtgtgata cctcaaatgt ccacttttgt tacctttgct gtccaaaagt gttaccctta
     4981 ctgtccactt ttgttacctt tactgtccaa aagtgttacc tttatacccc tttaatactt
     5041 actctcccaa gagattaagc ctcccctaac agatatatta acagatattt aacagatata
     5101 ttaacagata tttaataaag gaggattact cctctcttat ttcaaaatct taaaaataaa
     5161 aaatcttaaa aaaaactaac actatctagg agtttatttt gcttgtgtaa ctttgaataa
     5221 ttgaaaaaaa ggaaaaggag gaagcttca
//
LOCUS       DACDXE010000127         5247 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.130, whole genome shotgun sequence.
ACCESSION   DACDXE010000127 DACDXE010000000
VERSION     DACDXE010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.130"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1389
                     /gene="gadC"
                     /locus_tag="IWM23_002788"
     CDS             <1..1389
                     /gene="gadC"
                     /locus_tag="IWM23_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015082498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate:gamma-aminobutyrate antiporter"
                     /protein_id="HAP9885372.1"
                     /translation="GGILWFLPVALCAAEMATVEGWKDGGIFSWVSQTLGERFGFAAI
                     FFQWFQITVGFITMIYFILGALSYVINFPELNTDPFIKFIGVLVIFWVLTFSQLGGTN
                     RTAKIAKAGFVIGIVIPSIILFGLAAAYFIGGNPIQMAINAKAFIPDFSQLSTLVVFV
                     SFILAYMGVEASASHINELENPQRNYPLAMFLLVILAIALDAIGGFSVAAVIPQHDLS
                     LSAGVIQTFETLILHFNSHLHWLVKVIALMIACGVMGEVSSWVVGPSRGMFAAAQRGL
                     LPVFFRKTNKQDVPVPLIMIQGIIVTIWAAVLTFGGGGNNLSFLVAISLTVVIYLVGY
                     LLFFLGYFVLVFKKKDLKRTYNVPGKTVGKTIIGGIGFILSVFALLISFVPPASIAAS
                     EHRTYQIILLISFVVTVLLPFIIYEVHDKSAHTTIEEPTHVKAEDVNPAVYPAARGEH
                     HVTKNDEHILKH"
     gene            1402..2802
                     /locus_tag="IWM23_002789"
     CDS             1402..2802
                     /locus_tag="IWM23_002789"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003134257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HAP9885373.1"
                     /translation="MLYGKDNQEEKNYLEPIFGSASEDVDLPKYKLNKESIEPRIAYQ
                     LVQDEMLDEGNARLNLATFCQTYMEPEAVKLMTQTLEKNAIDKSEYPRTTEIENRCVN
                     MIADLWHAPNNEKFMGTSTIGSSEACMLGGMAMKFAWRKRAEKLGLDIQAKKPNLVIS
                     SGYQVCWEKFCVYWDVELREVPMDEKHMSINLDTVMDYVDEYTIGIVGIMGITYTGRY
                     DDIKGLNDLVEAHNKQTDYKVYIHVDAASGGFYAPFTEPDLVWDFQLKNVISINSSGH
                     KYGLVYPGVGWVLWRDQQYLPEELVFKVSYLGGEMPTMAINFSHSAAQLIGQYYNFVR
                     YGFDGYRDIHQRTHDVAVYLAKEIEKTGIFEIINDGSELPVVCYKLKEDPNREWTLYD
                     LSDRLLMKGWQVPAYPLPKDLDQLIIQRLVVRADFGMNMAGDYVQDMNQAIEELNKAH
                     IVYHKKQDVKTYGFTH"
     gene            complement(2967..4407)
                     /locus_tag="IWM23_002790"
                     /pseudo
     CDS             complement(2967..4407)
                     /locus_tag="IWM23_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328084.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            4800..5247
                     /locus_tag="IWM23_002791"
                     /pseudo
     CDS             4800..5247
                     /locus_tag="IWM23_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328085.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
BASE COUNT         1675 a          850 c         1001 g         1721 t
ORIGIN      
        1 ggaggtattt tatggtttct accagttgct ttatgtgctg cagaaatggc aacagttgaa
       61 ggatggaaag atggtgggat tttcagttgg gttagtcaaa ctttaggaga acgttttggt
      121 tttgcagcta tttttttcca atggtttcaa attacagtag gttttattac aatgatctac
      181 ttcatactag gagcgctgtc gtatgtaatt aattttccag agcttaatac agatccattt
      241 atcaagttta ttggtgtatt agtcattttc tgggtactga ctttttcaca gttgggcggg
      301 accaatcgta ccgctaaaat tgctaaagca ggatttgtca ttggaattgt tattccttcg
      361 atcattctct ttggtttagc agcagcctac tttatcggcg gtaatccgat tcaaatggct
      421 attaatgcga aagcttttat tcctgacttt tctcagcttt caactttagt agtatttgta
      481 tcttttatat tggcttatat gggcgtagaa gcttcagcat cacatattaa tgagctggaa
      541 aatccccaaa gaaactaccc attggcaatg tttttactag tgattttggc catcgcctta
      601 gatgcaatcg gcggtttctc agtagcagcg gttattccac aacatgactt gtctctcagt
      661 gctggtgtaa tccaaacatt tgaaacgctt attttacatt ttaacagtca tttacattgg
      721 ttggtaaaag tgattgctct catgattgcc tgtggcgtta tgggtgaagt tagttcttgg
      781 gtagttggtc catcacgagg gatgtttgca gctgctcagc gaggtctgtt accagttttc
      841 ttccggaaaa ctaataaaca agacgttcca gttccgttga ttatgataca aggaattatc
      901 gttactattt gggcagcagt cttaactttt ggtggaggag gcaacaattt atcattctta
      961 gtagcaatct ccttaactgt tgtcatttat ctcgtaggat atttactatt tttcctaggt
     1021 tactttgtat tagtttttaa gaaaaaagac ttaaaacgaa cttacaatgt accaggaaaa
     1081 actgttggca aaacgattat cggtggaatt ggttttattt tatcagtgtt cgcattgctt
     1141 atctcttttg taccaccagc ttcaattgca gctagtgagc accgaacata ccaaatcata
     1201 ctacttatta gctttgtagt gactgttctt ttacctttta tcatttatga agtacatgat
     1261 aaaagtgctc acacaactat tgaggaacct actcacgtga aagctgaaga cgttaatccc
     1321 gctgtttatc cagctgctag aggagagcac cacgttacaa aaaatgatga acatatttta
     1381 aaacattagg aggaattcat tatgttatac ggaaaagata atcaagaaga aaaaaactat
     1441 ttggaaccaa tttttggctc tgcaagtgag gatgttgact tgcctaaata taagttaaac
     1501 aaagaatcca ttgaaccacg aattgcttat caattagtac aagacgagat gttggatgaa
     1561 ggaaatgcgc gattaaactt agctactttt tgtcaaacgt atatggaacc tgaagcagtg
     1621 aaattgatga cccaaacgtt agaaaaaaat gcaattgata aatcagaata cccacgaaca
     1681 acggaaattg aaaaccgctg tgtaaatatg attgctgatt tatggcatgc tccaaataat
     1741 gaaaaattca tgggaacttc aacgatcggc tcttcagaag cctgcatgct gggtggtatg
     1801 gccatgaaat ttgcttggcg taaacgtgct gaaaaattag gtcttgatat tcaagcaaaa
     1861 aaacctaacc tggtgatctc ttctggttac caagtttgtt gggaaaaatt ctgtgtatat
     1921 tgggatgtgg aactgagaga agtcccaatg gatgaaaaac atatgtcaat taatctagat
     1981 actgtcatgg attatgtgga tgagtacaca attggtattg taggtattat gggtattact
     2041 tacactggtc gttatgatga tatcaagggt ctgaatgatt tagttgaagc tcacaataaa
     2101 caaactgact ataaagtata cattcatgtt gacgctgcat cgggtggctt ttatgcacca
     2161 tttactgaac ctgatctagt ttgggatttt caattgaaaa atgttatctc aattaattct
     2221 tcaggtcaca aatatggttt ggtatatcca ggtgtgggtt gggtcttatg gcgtgaccaa
     2281 caatacttac cagaagaatt agtatttaaa gttagttact taggtggaga aatgccaact
     2341 atggctatca acttctctca tagtgcagca caactaattg ggcaatacta caactttgta
     2401 cgctatggct ttgatggtta tcgtgatatt caccaaagaa ctcatgatgt tgctgtttat
     2461 ttagccaaag agatcgaaaa aactggtatt tttgaaatca ttaatgatgg atcagaattg
     2521 ccagttgtgt gctataagct gaaagaagat cccaatcgcg aatggacact atatgattta
     2581 tctgatcgtc tgttaatgaa gggatggcaa gtcccagcct acccattacc taaagacttg
     2641 gatcaattaa ttattcaacg cttagttgtt cgagcagact ttggaatgaa catggctggt
     2701 gattatgtac aagatatgaa ccaagcaatt gaagagttga ataaagctca tattgtttat
     2761 cataaaaaac aggatgtgaa aacgtacggc tttactcact aaaattcttc agagaaatat
     2821 aaaagaagtt aattaactaa taaaaggagt tgtgattacc acacagctcc ttaaatctat
     2881 ttgaataaat atatgatatt catttgttac actctcaatc ttagctgttg ttctattcat
     2941 cattactctt attgtaagta gttcagtcaa atttgcttgt tcaattgatc gataacagta
     3001 tgaattgttt gaaaagtaat cggatatata attgttttct cactacctaa gccttcttgg
     3061 tataaagaac tatcgcaaag tataatccgc tgagtacaaa agaaagtgtc gtctactatt
     3121 tctctaagtt gtttacaaat gatattacta atagaaaagt gttctcccaa aatgtcttct
     3181 aactgttgtc gatagtataa atacctaaag tctgacggag ctactatgaa cacttcaatt
     3241 ggagatttcc gtttatgctc atttaaaaga cttaaactaa tggttagttt acgaatagaa
     3301 ttctcattcc aacgcagaaa taaattgttt tgttttcgcc attgttcaag aatttcatga
     3361 acttctaagt acaaagcgcg ttgctcttca tttaacaagt aagtcttatc aggttgaaat
     3421 atctgaatat cagcccaaag tgagcggata aacgggagaa gtgctttatc aaaaagtatc
     3481 tgattttctt cagatatatt cagagataag ataagttttt gactgagctc aatcaccaaa
     3541 gggttatctg ctacatggtt ttgataaaca acgttcaagt ctttttgtaa caattcaaag
     3601 ttatcgcata gataatttct agagttgaat agaaagtaga tataaaggca ttcatcttcg
     3661 tcatgacata aagagaaaga aagttgctct aatcctttct gtattagctg aaacaaggga
     3721 gtatgtcggg caactctttt ttcttcctga ctaaaatata ttgggttagt cttagatctc
     3781 tgcaaaccga tagcgatacc taataaaata aattgttttt caactgtagg gtaacttctt
     3841 gaaaaaaaat gtttttctac ataatcaatc aattgttgac cgctttgaat aattgattta
     3901 tttagctgga tcaactctgt ccgatttgtc tcatagatta tcgttagtaa caaacttcga
     3961 taatcctttt caggaatttc aaaatggcca tcagcattta agtactgcat ttgtttaaaa
     4021 aaattaatca actcatttcg taagttgtaa actttgctta acgaaatgaa ggtagtatca
     4081 gctatattac tccaattgtg ttcccctgat aaataatgtc ctagtacatt gaaaaagtca
     4141 gagcgctgat agatgagttg agtgagtgcc aacctcgaaa acgaagagtc gaatgcgact
     4201 gtaaagctac ttctagattt ctccactgaa ataatttgct gatggagtat gtcggtaatg
     4261 cattccaagt cgttagtgac agacagtgag tgggcaaacg ttcaattgat ctgctatttc
     4321 gcgaatctga atggtccctt tttcgtgtaa ttgctcacaa atgtaaactt gacgaaaaat
     4381 acttttctcc agataggttt ccaacatagt actcctccta gtcattttat acataactat
     4441 tataactcta ccaatcggaa tagtcctgta atatattgaa tcaaaaatat aaattataaa
     4501 ataattagat ggggtatttg gtttaagtat cccttatata gtaataaatt tataggtgtg
     4561 attctcgaat taatacccga aaatgtgaga gaatttttat ggatttttta ggatacaaat
     4621 taattatttt tgcttttatc ctgattacca gtccagtact gtcggtatcc cattttgcag
     4681 caaaatcatg ttgataaagt gacaagaaga ctgatgagat atattatcca aattgagaag
     4741 ataattaatc ggattcttga gtatacccta ctttttaaga aagtagggta tacaagcgta
     4801 tgaataaaga cataactttc cctgtgatgg acgttcggct tgttccaatt accaaaatac
     4861 aggcgaacga ttataatccc aatcgagtag cgtctccgga aatgaactta ttggagcttt
     4921 ctatcattga agatggcttt actcaaccat tagtttgtta ttacaaggaa aaagaagaga
     4981 agtatatctt ggttgatgtg cttcataggt atcgtgttgc taaggaaaga ctcaacttaa
     5041 aagttgtacc ggtaacagtt attgataaac ccatataaga acgtatggct tcaacaattc
     5101 gacacaatcg tgcaagaggg acccatgaaa taagaggaat ggaaattgtc atccaaagat
     5161 tagtaaaaaa tggatggtca gacaagagaa tatccaaaga attaggcatg gacattgaag
     5221 aaatttttcg ctttaagcaa gtgtctg
//
LOCUS       DACDXE010000128         4861 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.131, whole genome shotgun sequence.
ACCESSION   DACDXE010000128 DACDXE010000000
VERSION     DACDXE010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.131"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1219)
                     /locus_tag="IWM23_002792"
     CDS             complement(<1..1219)
                     /locus_tag="IWM23_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAP9885374.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSFFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEM"
     gene            1280..1855
                     /locus_tag="IWM23_002793"
                     /pseudo
     CDS             1280..1855
                     /locus_tag="IWM23_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(2036..3229)
                     /locus_tag="IWM23_002794"
     CDS             complement(2036..3229)
                     /locus_tag="IWM23_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9885375.1"
                     /translation="MSEKRRDSKNRVLRSGESQRKDGRYAYKYVDTFGKPQFVYSWKL
                     VPTDKTPAGKREDISLREKEKEIQKDLDDGIDTIGKKMTVCQLYAKQIRHRGNVKHNT
                     KLGRERLMRILEQDRLGSCPIDSVKLSDAKEWALRMKEKGLSYKTINNDKRSLKAAFY
                     TAIQDDCIRKNPFDFQLSDVLDDDTEPKVPLTPAQEESFLSFIQGDKVYQKHYDAIVI
                     LLGTGLRISELCGLTDKDLDFENRVIIVSHQLLRNTGVGYYIDEPKTQSGVRKIPMNE
                     EVYQAFQRVIKNRKGAKPFIIDGYANFLFLKQNGYPMTAVDYGGMFGRLVKKYNKSHE
                     EALPKTTTPHAMRHTFCTRLANAGMNPKALQYIMGHSNITMTLNYYAHATFDSARAEM
                     ERLAA"
     gene            complement(3313..3516)
                     /locus_tag="IWM23_002795"
     CDS             complement(3313..3516)
                     /locus_tag="IWM23_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAP9885376.1"
                     /translation="MMNHDVPIWEKYTLTIEEASKYFRIGENKLRKLAEENPASGWVI
                     MNGNRIQIKRKQFEKIIDTLDTI"
     gene            3773..4015
                     /locus_tag="IWM23_002796"
     CDS             3773..4015
                     /locus_tag="IWM23_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885377.1"
                     /translation="MAHQPSGSAQTECVRMPYDAPPLSCGHIYQGAIRPPGKHRNGER
                     VSRFMLPGPVRQFVTIAWMSLISRRRFMASSRHTCG"
     gene            complement(3937..4170)
                     /locus_tag="IWM23_002797"
     CDS             complement(3937..4170)
                     /locus_tag="IWM23_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9885378.1"
                     /translation="MKNPNYKGIELLPLSVIEAARAGDAGAVERVLQYYSGYMNKLCT
                     RTLYDENGYPHVCLDEAMKRRLEIKLIHAIVTN"
     gene            complement(4177..4608)
                     /locus_tag="IWM23_002798"
     CDS             complement(4177..4608)
                     /locus_tag="IWM23_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAP9885379.1"
                     /translation="MDAIPRDYEARCMFDAFCKTVLRNEAKSYLAEMKRRRDREVSLS
                     SLSQADLDKLCTVDHYPSDSFTFSSHGYDLHINNELVAEAFAALPSMEQSILILHCVL
                     DMADGEIGGLVGMSRSAVQRHRTNTLSELRKQLKALMPKGG"
BASE COUNT         1325 a         1110 c         1003 g         1423 t
ORIGIN      
        1 acatttctac aaaaaaatag tgattctgtt ttgaaaattc gatttttagt tctgcatttt
       61 cgtaaacatc caacattttt ttcgcactgt atagacctaa cccgccattt tttttagagg
      121 tttttcctct tttgaccatc tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta
      181 tactaaaacg tgatccctct ttttcttcca gcagattgat cgtaatcgga ctctgatcgg
      241 ctacacgatg atcgaatgcg ttagaaataa aaatagataa acagcgaata aaaacaacca
      301 gatcaatcgg gattgttttc acttcgacag gaataactac ttggatcggg attttctccg
      361 attctgcttt ttctagaaat tttaaaaaga cacccctgac agctggggat acaatatttg
      421 ttagctgttg tatttgcggt ttttcaaaaa tagctgcaga atgatcttct aatatttcta
      481 aaaattccaa cgtttctttt ttattttcct gaagagtaat ttttaatgaa aacagtagac
      541 ttttataatc atgtttgaat tcctctagat gatggtaata ttcttcattt atttgataca
      601 gttgaagcga ttcctgatgt tgtctttctt ttacttggaa actgatcaac ataatggaaa
      661 aaaaaaaaga aaaaaaactg atgactatcg acaaaagaga tagaagcaga taattagatg
      721 agcggaagtg aacacttccc aataccaagt aaatcacgcc ccagcttaat aaaatacaaa
      781 tacctccaaa atagtgttca gttggataag taaaaagcaa gttgagaaat ctcgtctgtt
      841 tgtctatact ttttactaat agatagaaaa taaatgcgat aagtagtgca aaaaaacttg
      901 ctgttccaag atgtctcttt cctaaataat ccatcagatc aaaaaccaga aaaaagctga
      961 gtccagtgat agcaaaaaaa agattactga tcaaacagct gataaacagg tttttcgtag
     1021 taaaataaaa aagaaacatc aataataagc caactaggta aggaaagctc actttaggga
     1081 taggaagtaa aagtgtcacc accatcaaag ctatacaacc aaagctgttt tttactagca
     1141 gttgtttcga aaagaataaa tagataccta aatctaaaat caaagtaaag gtcaaataaa
     1201 gtgtcactgt tgcgaccatc ttattcctcc attgttttgt ttcaaaatgt acacatattt
     1261 ttttaatagg agcctgcata tgaaaaagta ttttatcata gaagataacc agtttcacag
     1321 cgaatggatc atcagagaat tggtcaatca tcatatcgag aatatcgtgc agctcacttc
     1381 tatctctcaa atcaatcagt tgatccagtt gggcactatt gataatcacg atgttttctt
     1441 tatcgatatc gacttgaagc aatcactcac aggtattgat gtcgccgaac aaatacgtaa
     1501 aaaaaatgca catagtaata ttgttttttt caccgcttat acgaattttg cagtagagat
     1561 catcaatcgg aaaatccttc cgattggata tatcagaaaa gacaggaatt tatctgaaca
     1621 aattcgtcaa gtgctagaac agatcaattg gaaagaacag cagatcgtaa atgtcgataa
     1681 aatctttatt gatcaaaaag gtggaagtct cgtcctgtat cctcgggaaa ttttatatat
     1741 tgaaagtatc aaaggtttga aaaaccaagt tatggtcaaa acaactacag aagataaatt
     1801 agtgaatact tctttaaaac aattgaagac agagttagaa aagtatgctt actttttctt
     1861 aaaaattata tgggatttta gattatcttt ttagaagttc ttgaatgtcg gtatagcccg
     1921 tgtttccggg cttttccggc atttttgata ttgttagata cttacaaatg ttcttattac
     1981 ctctcaccat ttccccctca aaagtagtaa atggagtagt aaaaccggcc ttgtgtcagg
     2041 ctgccagccg ttccatttct gcccttgcgg aatcgaatgt ggcgtgtgca taatagttca
     2101 gcgtcatggt gatattggaa tgtcccataa tgtactgcaa ggctttgggg ttcattcccg
     2161 cattggctaa ccgggtgcag aatgtatgac gcatagcatg gggcgtcgtc gttttcggca
     2221 acgcttcttc atggctcttg ttgtacttct tcacaagcct gccaaacatc ccgccataat
     2281 ctaccgccgt catgggatag ccgttctgtt tgagaaacag gaaattggca tacccgtcaa
     2341 taatgaacgg ctttgcacct ttgcggtttt ttatcactcg ctggaacgcc tgatacactt
     2401 cctcattcat ggggattttt ctgacgccgc tttgggtctt gggttcgtca atatagtaac
     2461 cgacgccggt atttctcaaa agctggtggc tcacgatgat tacccggttt tcaaaatcca
     2521 aatctttgtc cgtcagtccg cacagttccg aaatgcgaag ccctgtcccc agcagaataa
     2581 cgattgcgtc ataatgcttt tggtaaacct tatcaccttg aataaaggac agaaaacttt
     2641 cttcctgtgc tggtgtaaga ggcacctttg gttccgtatc gtcgtccaga acgtcactaa
     2701 gctggaagtc aaacgggttt tttcggatgc aatcgtcctg tatggcggtg taaaaagccg
     2761 ctttcaggga acgcttgtcg ttattgatgg tcttataaga aaggcccttt tctttcatcc
     2821 gaagcgccca ttcctttgcg tcggacagct tcacactgtc aatcgggcag ctccccagcc
     2881 gatcttgttc cagaatacgc atcagccgtt cccggcccag tttggtgttg tgcttcacat
     2941 tgccccggtg cctgatctgc ttggcgtaga gctggcagac ggtcattttc ttgccgatgg
     3001 tgtctatccc gtcgtcaagg tctttctgga tttctttctc cttttcccgg agtgaaatat
     3061 cctcccgttt acctgccggg gtcttatccg taggcactaa cttccatgag taaacaaact
     3121 gcggttttcc aaaggtatca acatatttgt aagcatatcg tccgtctttt ctctggctct
     3181 ctccagaccg caaaacgcga tttttgctgt ctcgtctttt ttctgacatc tcgtcatgct
     3241 cctttccatg acggaaagag ccgcatatat gcttataaat tattataaca tatctgcggc
     3301 cctttttcac tattagattg tgtccaaagt gtcaattatt ttctcaaact gcttgcgttt
     3361 gatctggatg cggttgccat tcataatgac ccagccagag gccggatttt cctcggccag
     3421 cttacgcaat ttgttttctc cgatacggaa atatttagac gcttcctcaa tcgtcagcgt
     3481 gtatttttcc caaataggca catcatggtt cataatgcag ccccctttca caaattcaaa
     3541 ggtttgtgtg atttaggggc atagacggac ggctgctggc acagctccac gggactttca
     3601 cccccgcgtg gttctcacgc ggccctaccc attgcctgcg acgcttttaa cgctcggact
     3661 gtggctataa gggagtatca tagtcctgcc tgtacgtcgt cgcccgcaag ccgccacgct
     3721 cacattgcgg cgtggtgttc ctcgctccgc ttttcccgta gggaaaagta tcatggcgca
     3781 ccagccgtcc ggctcggcac agacagaatg tgtccgtatg ccctatgatg cgccgccgtt
     3841 atcttgcggg catatttatc aaggtgcgat caggccaccg ggaaaacacc gaaacgggga
     3901 aagggtaagc cgtttcatgc tgccggggcc ggttcgtcag ttcgtgacga tagcgtggat
     3961 gagcttgatt tccagacggc gcttcatggc ctcgtccaga cacacatgcg gatagccgtt
     4021 ttcgtcatag agcgtccggg tacacagctt gttcatgtaa ccgctgtaat actgcaaaac
     4081 gcgctctacg gctccggcat ccccagcgcg ggcggcctcg atcaccgaaa ggggcaaaag
     4141 ctcaatgccc ttgtagttcg ggttcttcat ccgcgtctaa cctcccttcg gcatcaacgc
     4201 ctttagctgt ttccgcagct ccgataaggt atttgtcctg tgccgctgaa cggcactccg
     4261 ggacattccc acaaggccgc cgatctcacc atctgccata tccagaacac agtgcaggat
     4321 taaaatactc tgctccatgc tgggcagcgc ggcaaaggct tcggcaacaa gctcattgtt
     4381 gatatgtagg tcatagccat gcgacgaaaa cgtaaagctg tcgctggggt agtgatctac
     4441 tgtacagagc ttgtccaaat ctgcctgtga caaagagctt agtgacactt cacggtcacg
     4501 gcggcgcttc atttcagcca agtagctttt ggcctcgtta cgcagtaccg tcttgcaaaa
     4561 agcatcgaac atgcaccgcg cttcatagtc gcgggggata gcatccatct tctcaccccc
     4621 tttctgtggg ggacttaggt ggttctctcc ctttccgcta acaagacttt gacaaggccg
     4681 ccagctaccc gctaaatacc tcctttttga aaatttctca aaaaattttt ttggggacag
     4741 ccgggccgca aaagacgaca aaaatcgccc ggcaggtcgc aaacctaccg ggcgagaaaa
     4801 tgctttgcta tgctgctgat ttacatggta tagtacatac tcataaccgg aatagccctg
     4861 t
//
LOCUS       DACDXE010000129         4788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.132, whole genome shotgun sequence.
ACCESSION   DACDXE010000129 DACDXE010000000
VERSION     DACDXE010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.132"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            564..989
                     /locus_tag="IWM23_002799"
     CDS             564..989
                     /locus_tag="IWM23_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885380.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            2141..3118
                     /locus_tag="IWM23_002800"
     CDS             2141..3118
                     /locus_tag="IWM23_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9885381.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="IWM23_002801"
     CDS             3128..3625
                     /locus_tag="IWM23_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9885382.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="IWM23_002802"
     CDS             4001..4282
                     /locus_tag="IWM23_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885383.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="IWM23_002803"
     CDS             4284..4700
                     /locus_tag="IWM23_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885384.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DACDXE010000130         4744 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.133, whole genome shotgun sequence.
ACCESSION   DACDXE010000130 DACDXE010000000
VERSION     DACDXE010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.133"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..696
                     /locus_tag="IWM23_002804"
     CDS             25..696
                     /locus_tag="IWM23_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885385.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="IWM23_002805"
     CDS             755..1195
                     /locus_tag="IWM23_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885386.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="IWM23_002806"
     CDS             1207..1875
                     /locus_tag="IWM23_002806"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAP9885387.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="IWM23_002807"
     CDS             1939..2562
                     /locus_tag="IWM23_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAP9885388.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="IWM23_002808"
     CDS             2618..3004
                     /locus_tag="IWM23_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAP9885389.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="IWM23_002809"
     CDS             3217..4608
                     /locus_tag="IWM23_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAP9885390.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1580 a          782 c         1011 g         1371 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttg
//
LOCUS       DACDXE010000131         4720 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.134, whole genome shotgun sequence.
ACCESSION   DACDXE010000131 DACDXE010000000
VERSION     DACDXE010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.134"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..1097)
                     /locus_tag="IWM23_002810"
     CDS             complement(<1..1097)
                     /locus_tag="IWM23_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885391.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENA"
     gene            complement(1227..2603)
                     /locus_tag="IWM23_002811"
     CDS             complement(1227..2603)
                     /locus_tag="IWM23_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9885392.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2603..3259)
                     /locus_tag="IWM23_002812"
     CDS             complement(2603..3259)
                     /locus_tag="IWM23_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAP9885393.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3269..4546)
                     /locus_tag="IWM23_002813"
     CDS             complement(3269..4546)
                     /locus_tag="IWM23_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAP9885394.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            complement(4565..>4720)
                     /locus_tag="IWM23_002814"
     CDS             complement(4565..>4720)
                     /locus_tag="IWM23_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HAP9885395.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
BASE COUNT         1477 a          858 c          566 g         1819 t
ORIGIN      
        1 gcattctcat tagagaacac tccctttttt gtgacttttc tgaagctaga gtgaatactt
       61 tcaactgcat tagtagtgta catgattttt ctaatggcgc tgccataatc aaagagttgt
      121 tcaacatgag aaaagtttcg tttgcataca tctactgcac cagaataatg agaccaccga
      181 ttttgaaaac tgtcgaaggc agcgtgtgca gcgtttaaag aagaagcact gtagaatttt
      241 ttcatgtctc gacagacttc cttataatcc ttacttggga ggtatcgtaa cgcatttcga
      301 acaagatgaa caatacagcg ctgcacaatg acagaaggaa aaattgcttt tgcgccttct
      361 ttgagaccgg aaacaccgtc catcgaaatg aaaaaaacat cttctacacc acctactttc
      421 agttcatcaa atacttgcat ccagcgattt ttagattctg tttgattgag ccataggcct
      481 aaaatctcct tgtttccttt aagatcatag ccaagaatgg tgtacacagc atattcttta
      541 gcttcatagt tttcgcgtaa agtgacgtac atacaatcaa cgaatagaaa tgcataacac
      601 ttggctaatg gccgagcttg ccattcttcc aattcaggaa ggacggcatc cgtgatatca
      661 gaaatcattt cgtgggaaat atcaaagccg taaatatctt caacggttgc ggcgatatcg
      721 cattgactca ttcctcgtgc atacatggaa agaaccttac cttcgatgtc ggagacatct
      781 cgttttctat taggaatcaa ctcaggttca aaagaagcct cccggtctct gggaacatca
      841 atgtttactt taccaaagct cgttttaagc gtcttattgc cataaccgtt tcgtcgattg
      901 tcatgtttct ttggctcttt agaatgggcg tcatacccta aatggttatt caattctcct
      961 tgaagcattt tttcaaataa gggaccaaac acatctttta aaacatcttg catatcctct
     1021 acagattcgg gttgataagt attcaaaatg gattcagcta acttctcagc atcaggattt
     1081 cttttctttc tagccatcgt gtaatcacct cttgatctta ttttagaaaa ataaaaaccg
     1141 tgaagcaacc acccgcctag gattgatggt tacttacacg gtttatatta cactctcatt
     1201 tttggtgcta aaaagcaccg aaaattttaa ttattgtcaa ttaataattc ttttggattc
     1261 cttcttaata tattgaatga agaaataatc aatgatacaa gaagtactaa actcataaat
     1321 ataactacat aaatcatgaa tttaggtaat acattgatat ctaaacttga taatgtcttg
     1381 ttaaatcctt cagcttctgc cccaccacct aattggctag atgcagattg tttagctatt
     1441 tgtttagcta tatcaccagt aaccttattc aatatattat tcccaagctt gtcagctgta
     1501 tattgagcta agaaatagga accaacgaat gcggggattg atataaatac catctcaatt
     1561 aggaattgac caaaaatttc taattttgat atacctaatg atagtaatac tgctatttct
     1621 ttctttctag cattcatcca taagaataat aataatgaaa ctacaactcc tgcaaatatt
     1681 aatgagccaa aaaataattt atttgaaatt gagtaaatac ctgatataga ttgttgtaat
     1741 gcagggtaat ttgatgaact tttaatcaaa ttatattctc tccaatttat atctaatttc
     1801 ccaagatctt ttattacact atcaagattc ttatcacctt ttacaaagaa tgttgcatct
     1861 tggtatacag ctgtatcttc ggtattacca taaactttag cagctgtatg aatatcagtg
     1921 attaatgtat tttcgtatag ttcttgtgct gcacttactc caccgctatt atgcccatcg
     1981 aatagacctt taatttctac ttctactgtt tcatctgcac ctttctcatt atcagcatca
     2041 aataaattag attttatttt tatcttatct ccaactttaa ggttattttt ttcagctaaa
     2101 tctttatgca ttaagatttt atttttatct tcattttcta aatgctttcc ttctactaat
     2161 ttatatgctt ctgatacaaa ttttgtttct tttgttgagt cgttaacccc agttaacata
     2221 actgttcttt taaaattctt tgctctttca ggtgattgat tcgcaagagt ctcttgagtt
     2281 tcaataatat cataatcaac taaatctgca acactgttta ttctttttac ataggagtct
     2341 atactatctg tttgagatat ctttttaata tcttcacctt ttacatttcc cccacctcta
     2401 ggtgttcctg gatttacttg tctatttatc tccatagaaa aactatttgt tatattagca
     2461 aatgtttctt ttgaagctct gtccgtagca tctttaatag ataaactaat gagacttaaa
     2521 gttgacatag ataaaataac taatatgata attaatgatt ttaaactttt tctagttaca
     2581 taagcaaatg cattttttat cattattcaa cctccaaatt cattttattt acttttttca
     2641 actttttacc acttagttct aaaatgatat ccgcagaatc tgctacttcc ttactatgtg
     2701 ttacaactat tacacattta tttctatctt tagctaatgt ctttaatata ttaattattt
     2761 ctccagcagt aacactgtct agattaccag taggctcatc agctagtatt attggagcat
     2821 ctgataccaa tgctctagca atagctactc tttgttgctg tccaccagat aatttcataa
     2881 catttctttt tatttgtttt ttatctaaac ctagttcaaa caagatactt tcatctgctg
     2941 atttatttac taatctaata ttttcaattg gtgataaata atctattaaa ttatagtttt
     3001 gaaatactaa agatatatta ttttttctat gattactata tcctttattt tgtatatctt
     3061 cattcttaaa caatattttt cctgtttgag gtttatcaag tccagcaagt aaggaaagaa
     3121 gtgtagattt tcctgttcct gactttccta ctatcgcata aaacttccca agttcaaatt
     3181 tttgatttac tcctgacaaa actttttctt tagaatttgc ataactatat gctacattct
     3241 ttatttctaa tatatccatt attttctcct aacttatttt tgataagatt tctttaggct
     3301 tcttgattaa cattaatgaa gaagcaaata caactgataa aacaataata cttattaata
     3361 ttaaataact ttgtccaagt gttgttatat ttgacataaa actactatta tttattaaac
     3421 ttccaccgga aatcattgaa tcctctgagt taatgaatcc atctacaatt acttttagta
     3481 atacatttcc taaaaataag gaagatatta tacttggtat cgatatgaat attaactcga
     3541 atataaattg cattataatt tgtatcttag atgttccaat agataagaat atacctattt
     3601 cataaattct ttcccttaac catagaatca agattaatga aagaacaacc atcccaccta
     3661 acataataga ataagtcatt acttttatta tatattttat tccactcact gactctaaag
     3721 actcttcaaa cgcattagaa tctttttcaa caaaatactt tgactcatca attttaagct
     3781 ctttcaattt gtttaaggct aagtctgtag attctgcact accagaatac attaaaattt
     3841 tatttgcaat ttcattattt tctgaattat ttaatatctc ttggcttgtt gaataatcta
     3901 caaacaccat attttcacta aaatcagaag ataatcctgt atatgtttcc tgttttttac
     3961 cagaaaagat ccctataatt ttaaatttat gactttttat ttttccactt ttttcaatat
     4021 ctagtaattc aagatcaact tcatcaccta attttagatt gttttgttta gcaaattctt
     4081 catgaacaat aattgaattc ttatcatttt cttctatatt tttaccttct ttaattgtaa
     4141 atactccact actaaataaa atatttcttt tagtattatt tgtagcttcg agtgaaacaa
     4201 cattcttaaa ttcgtcagat aaatcttctc tatttattct ttgttctccg ctaactactt
     4261 tagcatcttt tagttttgct aatccatcat attgaattat tttttcttca atttctttta
     4321 atttttcaat atctttaaat tgattaacat taaaatattt accatctttt tttgttattg
     4381 atattgaaga attagaactt tcatataaag ccttttctat ttcattgctt gatttcatta
     4441 ttgttaaaca agaatacaag caagaaagaa ctatagttaa tatgataaaa attattaaag
     4501 ttctattttt ctttcttgta atatatgcta tagcattttt tatcactttc ctaactactc
     4561 ctctctattt tcttactcca taactaattg catccactgg acaagcattt ttacatcgtc
     4621 cacatctgat acactccaga tgattgcaat tctcaactga atctttattc atttggcaaa
     4681 cttttttaca ttttccacaa ttaatacact tatccttatc
//
LOCUS       DACDXE010000132         4709 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.135, whole genome shotgun sequence.
ACCESSION   DACDXE010000132 DACDXE010000000
VERSION     DACDXE010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.135"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..356
                     /locus_tag="IWM23_002815"
                     /pseudo
     CDS             105..356
                     /locus_tag="IWM23_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            449..1243
                     /locus_tag="IWM23_002816"
                     /note="aph(3')-IIIa"
     CDS             449..1243
                     /locus_tag="IWM23_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9885396.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            1653..1764
                     /locus_tag="IWM23_002817"
                     /pseudo
     CDS             1653..1764
                     /locus_tag="IWM23_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            1785..1868
                     /locus_tag="IWM23_002818"
     CDS             1785..1868
                     /locus_tag="IWM23_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9885397.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM23_002819"
     CDS             1993..2730
                     /gene="erm(B)"
                     /locus_tag="IWM23_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9885398.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(2993..3856)
                     /locus_tag="IWM23_002820"
     CDS             complement(2993..3856)
                     /locus_tag="IWM23_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HAP9885399.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
     gene            complement(3858..4130)
                     /locus_tag="IWM23_002821"
     CDS             complement(3858..4130)
                     /locus_tag="IWM23_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9885400.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            complement(4147..4362)
                     /locus_tag="IWM23_002822"
     CDS             complement(4147..4362)
                     /locus_tag="IWM23_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HAP9885401.1"
                     /translation="MIVGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIET
                     AKNGGNVKEVMDQALEEYIRKYLPDKL"
     gene            complement(4454..>4709)
                     /locus_tag="IWM23_002823"
     CDS             complement(4454..>4709)
                     /locus_tag="IWM23_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HAP9885402.1"
                     /translation="IGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKVS
                     TWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
BASE COUNT         1445 a          796 c          865 g         1603 t
ORIGIN      
        1 tcttgggagt tttgagacta ctatatgcgt ttttttgttg atttaccaac atgtttagac
       61 attttcctct aggaaaaaat taactagcac cacgggttat cttttatata gaagatatcg
      121 ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc gcttatcaat atatctatag
      181 aatgggcaaa gcataaaaac ttgcatggac taatgcttga aacccaggac aataacctta
      241 tagcttgtaa attctatcat aattgtggtt tcaaaatcgg ctccgtcgat actatgttat
      301 acgccaactt tgaaaacaac tttgaaaaag ctgttttctg gtatttaagg ttttagaatg
      361 caaggaacag tgaattggag ttcgtcttgt tataattagc ttcttggggt atctttaaat
      421 actgtagaaa agaggaagga aataataaat ggctaaaatg agaatatcac cggaattgaa
      481 aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa ggaatgtctc ctgctaaggt
      541 atataagctg gtgggagaaa atgaaaacct atatttaaaa atgacggaca gccggtataa
      601 agggaccacc tatgatgtgg aacgggaaaa ggacatgatg ctatggctgg aaggaaagct
      661 gcctgttcca aaggtcctgc actttgaacg gcatgatggc tggagcaatc tgctcatgag
      721 tgaggccgat ggcgtccttt gctcggaaga gtatgaagat gaacaaagcc ctgaaaagat
      781 tatcgagctg tatgcggagt gcatcaggct ctttcactcc atcgacatat cggattgtcc
      841 ctatacgaat agcttagaca gccgcttagc cgaattggat tacttactga ataacgatct
      901 ggccgatgtg gattgcgaaa actgggaaga agacactcca tttaaagatc cgcgcgagct
      961 gtatgatttt ttaaagacgg aaaagcccga agaggaactt gtcttttccc acggcgacct
     1021 gggagacagc aacatctttg tgaaagatgg caaagtaagt ggctttattg atcttgggag
     1081 aagcggcagg gcggacaagt ggtatgacat tgccttctgc gtccggtcga tcagggagga
     1141 tatcggggaa gaacagtatg tcgagctatt ttttgactta ctggggatca agcctgattg
     1201 ggagaaaata aaatattata ttttactgga tgaattgttt tagtacctag atttagatgt
     1261 ctaaaaagct ttaactacaa gctttttaga catctaatct tttctgaagt acatccgcaa
     1321 ctgtccatac tctgatgttt tatatctttt ctaaaagttc gctagatagg ggtcccgagc
     1381 gcctacgagg aatttgtatc gataagaaat agatttaaaa atttcgctgt tattttgtac
     1441 atttaacttg acggtgacat ctctctattg tgagttatta gtggtacagt tttcaaccgt
     1501 tttaattata aaaaagtggt gcatttttaa attggcacaa acaggtaacg gttattgcag
     1561 gtgtatttct tatctatggg tttaacatgg attttatcat taaaatcatg agtattgtcc
     1621 gagagtgatt ggtcttgcgt atggttaacc ctaaagttat ggaaataaga cttagaagca
     1681 aacttaagag tgtgttgata gtgcattatc ttaaaatttt gtataatagg aattgaagtt
     1741 aaattagatg ctaaaaattt gtaattaaga aggagggatt cgtcatgttg gtattccaaa
     1801 tgcgttatgt agataaaaca tctactgttt tgaaacagac taaaaacagt gattacgcag
     1861 ataaataaat acgttagatt aattcctacc agtgactaat cttatgactt tttaaacaga
     1921 taactaaaat tacaaacaaa tcgtttaact tctgtatttg tttatagatg taatcacttc
     1981 aggagtgata acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa
     2041 gtactcaacc aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt
     2101 ggaacaggta aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct
     2161 attgaattag acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt
     2221 gtcactttaa ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa
     2281 attgttggga atattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa
     2341 agccgtgcgt ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat
     2401 attcaccgaa cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag
     2461 ctgccagcgg aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc
     2521 cgccatacca cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa
     2581 tgggtcaatc gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa
     2641 cacgccaaag taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat
     2701 agttatctat tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa
     2761 gttacacgtt actaaaggga atgtagataa attattaggt atagggcacc tctaataact
     2821 accaattaat tcacctcaaa atgaaattag aaaaaaacag cgaaattttt aaatctattt
     2881 cttatcgata caaattcctc gtaggcgctc gggaccccaa catgaggaaa tgtttttata
     2941 aaaaaagagt agttaccgtt ttttggtaac tactcttttc cttttttaag gtttaaatac
     3001 ctggaagttt aggtgttttg ggtattggtg gtgtaggtgg ctgcaagctt tccaattttt
     3061 gttgaattgc tttaaattca ggtgtctctt ggtgtttatt tagaaccatt ttttgctcta
     3121 ttcgttctaa agtcggttga atttctttcc ctgatacttt acgattcaat tctttttcta
     3181 aggtttcttt tggactaata gaaggtgttt ccaaacttga atagagtttt actccttctc
     3241 tgttatatag ccttatatcg ctaaataagc ccgttttatg aagggtttct aaattggtcg
     3301 gtaagttttt gacaacaata tcatgcgctt gttttggtgt tgccctggct gtcattggat
     3361 catctgcata catggtttca tatcgttcaa ttgttcctaa atatgagttg attttaggta
     3421 ctgccatgac atacattttt gtttcataac ctttggcttg aagcattgtt gcggtttgaa
     3481 taggaacgtc tgttgttcgt cctgtacctt cgatcaccaa attataccct tgatcgctca
     3541 aacggcttat gatcgcttct gtcatgcgat tagaataagg ggtaacgtgt tttactacgt
     3601 ctttttcata aagtttcact agttcatcaa aattagggtg ctgttgttta aaggtatcat
     3661 tatcaatgac aataacattc ccttgtgttt cttcaaaaat tgctgatcgc aaactggttt
     3721 tccctgaccc tggttgccca ccaagtaaaa aagcggttgg cgattcaacc gctttttttc
     3781 cttgaatcaa ttcttctaaa ttatcattta agcgattctc aaattgtttg tcagtaaaat
     3841 tgactatatt tgccatatta agccactttc tctttattca aaacttgttt tgagtaacga
     3901 ttcattgacc gccaatactc atgaacggct tgtaattctt ctaaagaata atcaaaaaga
     3961 tttacacgtt ttgcaagctt taattggttt aataaattga cttccaataa ttgagagtca
     4021 tttttattta attcatggtt caaaacatag tttaatactc gattataaac gcttgccgat
     4081 aattcgttaa tgatctctat ttcaaatgtt ttttcatacg taactgccat ttcattttca
     4141 ctccttttaa agtttgtcag gtaaatattt ccgtatatat tcttctaagg cttgatccat
     4201 aacttctttt acgtttccgc cattctttgc tgtttcgatt tttatgatat ggtgcaagtc
     4261 agcacgaaca cgaaccgtct tatctcccat tatatctttt tttgcactga ttggtgtatc
     4321 atttcgtttt gctttttgtg cgcctaaatt tcccacaatc actcacttct ttctatttct
     4381 tcttactctt attttatcat caacaatcac aaatcacaag tgatttgtga ttaatcactt
     4441 gtgatttgtg attctattct ttttcgtttt ctaattgaat aattcgctca agcatttcaa
     4501 aaaatacgtt cttatacatg gataaaactt ttttgtcata gcctttatgc tctgtaatgc
     4561 cgtttttaga ccaggtactt actttattac ttcgcttgat aatattttgg aaaaccaagt
     4621 tatcttcttt atgttgctta tacagttctt ccaggtttga ttttatcgtt gcgctgtccg
     4681 tatcaactaa ataaggaaca aaaccgatc
//
LOCUS       DACDXE010000133         4632 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.136, whole genome shotgun sequence.
ACCESSION   DACDXE010000133 DACDXE010000000
VERSION     DACDXE010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.136"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            174..518
                     /locus_tag="IWM23_002824"
     CDS             174..518
                     /locus_tag="IWM23_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAP9885403.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="IWM23_002825"
     CDS             519..938
                     /locus_tag="IWM23_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAP9885404.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="IWM23_002826"
     CDS             complement(1030..1380)
                     /locus_tag="IWM23_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885405.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="IWM23_002827"
     CDS             complement(1346..2566)
                     /locus_tag="IWM23_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAP9885406.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="IWM23_002828"
     CDS             2932..3498
                     /locus_tag="IWM23_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAP9885407.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="IWM23_002829"
     tRNA            3643..3730
                     /locus_tag="IWM23_002829"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4632
                     /locus_tag="IWM23_002830"
     CDS             4064..>4632
                     /locus_tag="IWM23_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9885408.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1682 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat gg
//
LOCUS       DACDXE010000134         4614 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.137, whole genome shotgun sequence.
ACCESSION   DACDXE010000134 DACDXE010000000
VERSION     DACDXE010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.137"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1365..2405
                     /locus_tag="IWM23_002831"
     CDS             1365..2405
                     /locus_tag="IWM23_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAP9885409.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3229..3561
                     /locus_tag="IWM23_002832"
     CDS             3229..3561
                     /locus_tag="IWM23_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAP9885410.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4180..4521
                     /locus_tag="IWM23_002833"
     CDS             4180..4521
                     /locus_tag="IWM23_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAP9885411.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1694 a          610 c          787 g         1523 t
ORIGIN      
        1 aaaaccccat cagcctgcac gctgatgggg tttggtgata taatgaaata cctctttgtt
       61 actcataatt gtaccataat tagtcacgaa tgcaaccaat agaaaaaact acttagaacg
      121 gtagatcatc gctaataaca atgcattgtc caccaaatgg attttgtgaa gcattaaata
      181 cattgttatt aattgtcgct gcttttaact aggcctctcc ccttctatta taaaagaaga
      241 tatatatcat ttcttaatta cctactacct taaaccaaaa tttaagtact acctaattta
      301 agtattactt aaacaatcta aaaaatagtt atcccaattg ttatgctttt ataatacgat
      361 tatttgtaac aaaatgaata ttatataatt aaacactacc atatttaggt agtgtttaat
      421 tattagcaaa acaaaaaata tttattctat tctcttaact tcaactaagt tatgctgcac
      481 acctttatat aaatcatatg caactcatta gctagacaaa ctacaagcat gtagttttat
      541 ctgttaatta tgtactacct aaataaagta gtttataatt agcaagtata tatatttaaa
      601 aatttaagat taattttttt atgacttgta caaatttatc tgatattctc ttattaagaa
      661 ctacctaatt tgggtagttc ttatttttta cattttaagt agtatttaat tgcaatttgg
      721 tagtattttt ttattatctt attcttagaa cggtagtact taatataaat ataggtagta
      781 tctaatttaa gtattaatgg tagtatctaa attaacaggt agtatgtaac tactttaaag
      841 taattttttt cagattaaca cagtagtatc ttattaaata agttaatttt aatggtagta
      901 cataattatt atatatatct gacattggta gtacttattg tattttttag tagtatataa
      961 tctattgttt tctagcctaa agccttgtct tataagaata atataatata tactactgaa
     1021 ataaggtagt atatattact agtaatggta gtatgtttta tatgcataag gtagttctta
     1081 atttattttt tgttttgaca ctggtagtac ttaattatct ttaattttaa aactaaattt
     1141 tattcatacg tttttctggt agtatttaat tataaataat atttatttaa agtagttctt
     1201 aatgacttta aaggtagtac ataattcatt ttatttctga ttttaggtag tatttaatta
     1261 aaaaacagtt ttaatgcgtt tgcatttttt atttatcttt gctattttcg aaaagagtag
     1321 gtaattttac ataaataaaa caacgtgaaa gtagaggatc tacaatgact gattttaaat
     1381 tttttaaggc ggatagagtt tacaacgaat tattttatca atttccaaaa gtctttattg
     1441 tttctgacga atacaaaaaa atgaaagatt caactaagat tgcctatatg cttttaaaag
     1501 caagattaga gatcgcaatc agcaaacggc aaatcgatga agaaggtaat gtttatttta
     1561 cttatacgac aaatgaacta tgtagagtat taaactgcca aaaacaaaaa gcgatagcaa
     1621 tcaaaaaaga gttggaatcc tttggtttat tattacaaaa gcagatggga tttaacaaac
     1681 agttagggaa aaataatcct aatagactat atctagcaga attaaaagtc tcagaaaatg
     1741 atatctactt actcgaaaaa tttgatagag agaataggga aaacgttgat aaatcagagg
     1801 gtatgaaaat catacccacc ctcgacgaaa aatcagacgc tgaatccctt ggggctcaag
     1861 agggtatgaa aatcataccg tgccaaaacg ttgataaatc agagggtatg aaaatcatac
     1921 cagaacttaa taataatata ttagacacta atagacacaa tatagacact gaaaaagacc
     1981 gcctacaaga tcaattgttg ttagacaatt ttgagacaat tatgacaaac gacagcattg
     2041 ctacgtttgt ccctgaacga gtattaaatt tgataaaaac attttcttca agttacagtg
     2101 aagctcaaaa aaccgtccag actattcata atgcaaagaa aaaagctgaa atagaaagtg
     2161 gtatttcgat agtttttgaa gaactcgata gttattatgt caatgcagaa caagaattat
     2221 acacgacact gttaaaagcc tatcaaaaat taaaaaccga aaaagtcgaa aatatccaga
     2281 acctgatttt tgtctatgta aaaaattggt ttatcgaaaa accaatagct gctaaagtat
     2341 caagtgaaaa acgtttgaat tatgaaagct ccccaagcac tattacgaaa gactggttag
     2401 agtgaaactt tactgacaaa gtgattgttg cttcatggag taacattaaa attaaaaatt
     2461 ttttattatt ctaattatat tacgaatatt acaaaataat agctatttta atagaattta
     2521 taagttaaaa tattatgata aaaatcacaa acgtagcctt ttacgtcata agtgttaaaa
     2581 gaaataatta atttcaaata tagaaaaggg aacgtatgtt tgcatactcc ttttttttga
     2641 gttataataa tacgcgaaga gagataattt cattatttaa acaagctaaa aatcttgata
     2701 ttacttcttt tcaggcaata cgaatattat tcgcttacat tttttgtata aatttaattg
     2761 tctgctatgc ttgtgatgaa ttgattttcg ttctgcactt ttggatgata aatttggaca
     2821 attgaaaata caaaaaaaag aaaggcggta tgaactcgga aggctggtaa ccttcgtcag
     2881 cgttcataca cggcattaag atttggccct taatgcgtta acctttcagt gttacggtat
     2941 tacccgcaat ggatttagta taacagatat tcaaaagtga taccactttt tttggctgtg
     3001 ttaaggttaa aaatacgtga tattacgatg aatctatgcc taagcacaat gttataaaaa
     3061 tgtccgtgtg gctctttgcc gtatgacgaa cgctgaaatt agagggaacg catgtttgga
     3121 tgcgttccct cttttttgtt tgtcatgaac gaaaatcaat tctctatttt tgaaaaataa
     3181 caagaaaaac ttgttaaaaa tatttaaaga aattgggtgg tttattttat gtcatataca
     3241 ggaattttga gtctcaaaga tatttgtcac tacggtaaac ggtgtaccgc tacagagaaa
     3301 atcacgaaga aactatcaac tggccaaagt aaaaccgttg tgcagtgtaa gaaatatata
     3361 atccagaaag acaaagtctc agaggaaatg atttattaca ttgggaaaca aaagcaaata
     3421 attttaaaag atcctatccc cttaaaagag ctatatccaa caatcaaaca tgtttatgac
     3481 caaaacggtg tactgattgg tcgaagaaaa aatggagtct tgcgttgtac cgcaaaaggg
     3541 atggggcgtt tgatcggctg aattgatcat tctcataact tgtattttat cgtttttcgc
     3601 ttgcttgaaa atattgtcga gacttctaaa ggaacttaga gtcactagaa aaataaggag
     3661 tagacttgat ctatcaacaa ttcccttcgg ggagtgacgg ggtaggtttt aacgattggt
     3721 aacgtaaaaa attggtcaat cgagtaaatg cgcttgtttg gatagtggtg aaatgaattt
     3781 gtgtaaataa taagcgcaga gtaaacaaga gggaatttag aagttagaca agttagaata
     3841 gcgtctagat taatcatgag cagctactaa gggatctaga ctttgacgca aactcatgga
     3901 ataaggagta ggtcttctcg taatggggag gacgcacaag aaattgtgtc gtgtgctgca
     3961 gtacatgtag atacttgcga ggacgttgat tattggatca atcgagcatt taccgctgtc
     4021 tactagggtt tctcgtttcg gacgccataa acgagtcgaa attttttatg tatttttaag
     4081 taaacgaaaa acgataaaat atgaacaagt cctgaataaa gtgaaaaaat tgatataaaa
     4141 catagagaaa atgaaattta cgtaatggag gaatgacaaa tgaaaaatta tgatccgaat
     4201 atacgattgg gcacacatac tattaaggtt tctttccaac gttgggatta caaaggattt
     4261 gtgacgttta gaagaggcgg aaattgtaaa ggattagatg ttttagcatt agacgaggat
     4321 gatttatacg atcaaacatt gactgataat ccaattggat tcggcttgct acctgaagat
     4381 gatgaaggta atgaatggtt taaaatgacg cttatgaatg ataatggaga cgaattatct
     4441 gttgaagata cttggtcata tttgagtgat tatattgtca gctttgaaat cattgaattt
     4501 gtagcggata aagaagaata aaaactggag gaataaattt tacagataaa ggcttagaag
     4561 gagtcacgat gattaataaa aaggtagtat taccttaggc attgagtaag gaat
//
LOCUS       DACDXE010000135         4611 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.138, whole genome shotgun sequence.
ACCESSION   DACDXE010000135 DACDXE010000000
VERSION     DACDXE010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4611
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.138"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(86..340)
                     /locus_tag="IWM23_002834"
     CDS             complement(86..340)
                     /locus_tag="IWM23_002834"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9885412.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPIMDRLQLTRQKN"
     gene            complement(451..705)
                     /locus_tag="IWM23_002835"
     CDS             complement(451..705)
                     /locus_tag="IWM23_002835"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885413.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
     gene            complement(898..1173)
                     /locus_tag="IWM23_002836"
     CDS             complement(898..1173)
                     /locus_tag="IWM23_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HAP9885414.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(1145..2098)
                     /locus_tag="IWM23_002837"
     CDS             complement(1145..2098)
                     /locus_tag="IWM23_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9885415.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            2710..4203
                     /locus_tag="IWM23_002838"
     CDS             2710..4203
                     /locus_tag="IWM23_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepR"
                     /protein_id="HAP9885416.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            4338..>4611
                     /locus_tag="IWM23_002839"
     CDS             4338..>4611
                     /locus_tag="IWM23_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAP9885417.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELR"
BASE COUNT         1492 a          774 c          802 g         1543 t
ORIGIN      
        1 ttgcttgaat tcttgtgaaa aagttcttcg tgttctctta gacataaaaa ttcctcctgg
       61 tatgttttct tctagtctac acaccttaat ttttctgtct agttaattgt agcctatcca
      121 tgatcggttt attagacctt tgaggtctat attttttaac tacaatcgac cttaaattta
      181 attgtttcat tagcttttgg acatgtttaa gagatactga aatcccttct tttaataaaa
      241 cttgatggat ctttgtggca ccatagcgtt gtttattcgt aaaaaaaatt gttgaaatct
      301 tctttttcaa cttattattt ctgatttctg tagctgtcaa cggtttattc ttataaaaat
      361 aatagacact tctagggatt tctaataacc gacacaacaa agaaacagtg taatccttac
      421 accatttttc aataaattga acaagcgaat ttaggttttc cgagagaata tggctgcggc
      481 tttttttaag atttcaagtt cctcatttaa acgagcattt tcttttctca gttcagctac
      541 ttcttttcca gtcagtcctg tggactgatc aggtaaatat aaattcttcc atttgtaaat
      601 cgttgcttca gataagccat attctttcgc taaccctcga acagaacgtc cagtttgatt
      661 cagttcaaca atcatttgtt taaaattttc ttcatatcgg gtcataaaaa ttcatcctct
      721 cgtagataag tttaatttat tcttgtctct acgaaaagct gtctaagatt tgatactaac
      781 accaaatgtt gtataccccc aaacaataca catattttga aggattagga ttaataaagc
      841 taaaaatata gtaagtatac taaatatatt tactatatct agtatactta ctatatttta
      901 atcttttgat cgtctattat atataatccg ttcctcgtcg tttaaagtat caacataggc
      961 gtccattgca agtttgacta tttcatacat aggaatatct tttgcataac taagatcccg
     1021 aatggttttt agtacgggag gatctacttg aactggtttt ctttctttcg cagtgtactt
     1081 tcgttcccct aactcgacat taatatcatc tttaattgaa atatcttcag caccaatttt
     1141 cttcttatct ttgttcgttg gtaataatgc catttagttt aatctccttt ccaagaggta
     1201 ggatatgatt ggccattgag tcagcatatt taggtgtata atcaaatcct tcaatatcac
     1261 catgttgttc atagtattca attctttctt ctaattctgt aaagatgtct gcaaaaatag
     1321 cccaccatct tcgatctaca taatcattta attggatacc attctcacca tacatttcta
     1381 accgatccga gcctttaaca atgcttttaa aaatgtttga ttcaccatac ttatcaattg
     1441 ttttttgata gtttgcgtgt tgagaggaac gtcgcactga gaaaagaaca ggcaaaattc
     1501 caatgatttg aagatgagac tcttcaaaat tcgtgacaat cggaactaaa actttattaa
     1561 taaaatcttc tgttccttcc attgcgtacc gttttaattc ttgcattgca ataatataat
     1621 ctgcagcagt taagaatgca cgaaccacat tatctgttga tggtgggcag tcgataaaga
     1681 tataatcgaa attttttctc aattcttcta aaggtttaat gaaggccaat gatctcttac
     1741 gacgatcagg atatttttcg gacaagtatt cattaagttc ttcagatcca ggagagccag
     1801 ccataatata taaatgtgga cttacttgag taatacctga aacccagtcc ccttttttga
     1861 ttgctgcaga catggatcga tcaaagctag taatttgata cgttgatcca atgttggaag
     1921 tagtatttct ttgttggtca taatcaatta gcaacacgtc tttttcaaaa agttgactag
     1981 aaacaatcgc taataaatca ttgtctgtag tcttaccaat gccgcctttt ctacctgtaa
     2041 gcattacaac ttttcctagt ttatctgatg ttttaaatgc agtccgtaag ttttccatag
     2101 ttccctccaa tttcctatac taaatatatt tagtatactt catatactta atatattcga
     2161 tatatctaat atactccaaa ttgataagaa ttcagacttt ataaggaata tagtggatat
     2221 actaaatata tttgctatat tcagtatatc agatatattc aaaatgtcaa aatatatttg
     2281 tgcgttttgc gttgacaatt tagagtgaaa agaggtaaac ttaattctat agaaaccaca
     2341 gataattcac tatttacaaa gtagcaaaag cagtggtact ctttaaacag aaaaattaca
     2401 taaaaaaaag agacggaaat aattgatttt agcagaatca attatttctg aatcacatgg
     2461 ttaggacatg tgttcacgct cgtctcaaga atatgtaagt agctaccaac tacttatatt
     2521 aaaagttgat cggatacctt ttccgattgc ctttattcta ccaaaaaaag gcgagcaact
     2581 caacagaaga acgtttattt agtgtactta aattaaatat tcttaaacac tgaagagaga
     2641 acacatacta tggctgagat taaagctaaa ctgtgttctc tttttgtttg agaaaggacg
     2701 ttacaatcga tgttgagcga aatttattca gctattctta aaaacggctt acgtacatac
     2761 aaatatcgaa acagcaccat gaaacctgca ggtcatattg attccagtag gagtggtgct
     2821 atctttgcgt accgtagcaa gcaattaatg aatgttgggc gtggtatggt cattacttcg
     2881 gaagaagcta ttttagagaa cgaaaagaaa ctaacgcatt ggacacctaa tacctatcgc
     2941 tttggtactt atgcggatga ttatcgaaca gtggttaaag gtcattctga gaaaaactta
     3001 agtcaaatca acacatttgt agttgatatc gattcaaaag agaaccatca aggcgaaatt
     3061 attttagcct gtttagatca agtgggctac atgccaacgc taattttaga atcagatcat
     3121 ggttatcaag tttattttgt actaaagaca ccaggctatg tcacaaagaa aagcaatttc
     3181 aaggtgatcg atgtagcaaa aaaaatttca aaaacaattc gtctacagtt ggctgaaaaa
     3241 ttgtccggag tggatctagg atgtaaccac ttcggaattg ctcgattccc acaagaaaat
     3301 aatattgttt ttaaagaaat gaactatcag tattcttttg ctgactggat tcaatggtcg
     3361 atgaagcttg aatcagatca aaaaacaaaa gaagatcgag aacgaaaact aattgtcttt
     3421 cctgagaaaa aagaaattcg tcaagtagat gaaccgtggt tcgatatgtt acttcataaa
     3481 gcaaacatcg tcggcggaga aggacgtctt ggtcgaaata atgtgatttt cacgctttct
     3541 ttagcttatt actcttcagg ttatgcattg gatacttgcg aatacaatat gttcacattc
     3601 aacgaacggt tacaggaacc gctatctgaa gcagaattag taaagattgt acgtagtgct
     3661 tattctggta attatcaagc agcaagcaga gaattcatta ttgaattgtg ccagcaatgg
     3721 gtttcaccaa ccttaaaaaa taatgatctg ttcattcaac gacgtggctg gtggaaattt
     3781 aaaaagccac gagatcaaag aaaatatagt cataaaaatg aatggaaaga agatctattg
     3841 gcttatctaa gcgaaaaaag tcttgctagt gagccttatt tgaacctgtc aaaaaaagaa
     3901 ttggcttcac aactaacgat gcccaagcga acacttgata agatcttaaa tgagttacga
     3961 gaggagaata aagtcttcta caaggtaaca cgaggaagaa atggcggaat gatcattgct
     4021 tcagtcaaaa cgttgttcgc gagggttatt caactaagta aagaaaagag actagattat
     4081 tcaacaagga tcacagaggt atttggactc aacgagagct tagtagaaga gacatttaga
     4141 caattatcaa cccccttgaa ccctcttaaa gaagtgaatc tttttgaagt agatacaggt
     4201 tgaaaaataa aaggtgcaaa ttgccttata caatatatct atgatacttc tgtaggtgtt
     4261 gctccctttg cctagctacg tgtaggtggt atacttataa ctgcgtatgc agtcttatgt
     4321 atcatggagg aagttgtatg tcgttaaaaa attatgtcta ttcacatcct gttaatgtgt
     4381 ttatcattgg tcgtttggga ttatctgtgg aagtgttctg tgagctttat ggttttaagc
     4441 aggggacttt gtcttcttgg gttaccaggg aaaaaacagt tgcttctttg ccgattgagt
     4501 ttcttcatgg gttgtcctta gcttctgggg agactatgga tcaggtatat gattgtttgt
     4561 gtgtgttaga gcaggagtat attgagtata aaatagctaa cgagttaaga a
//
LOCUS       DACDXE010000136         4468 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.139, whole genome shotgun sequence.
ACCESSION   DACDXE010000136 DACDXE010000000
VERSION     DACDXE010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4468
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.139"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..1047
                     /locus_tag="IWM23_002840"
     CDS             316..1047
                     /locus_tag="IWM23_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAP9885418.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1192..3219
                     /locus_tag="IWM23_002841"
     CDS             1192..3219
                     /locus_tag="IWM23_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAP9885419.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3327..4424
                     /locus_tag="IWM23_002842"
     CDS             3327..4424
                     /locus_tag="IWM23_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAP9885420.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1568 a          717 c          923 g         1260 t
ORIGIN      
        1 ataatataca ttttttattt aattataatt aatattgatt gaagtctagg cagtcaagtc
       61 acattttgat agataggagg gggacttacg aattatttta aaggctatga cagctgtatt
      121 tctagattgt gtttgcatca agttgcaata aaaactagaa atcaaatcgg gctgataatc
      181 atttataact aatcgataca taggaccaaa tataggtcta tgttctttta tttcataagt
      241 atcgtaaaac agaattttgc tatggattaa tccaatcgac tgatatgttt agtaaataaa
      301 gaaagaggaa tgtcaatgat gaaaaaagaa atcgtattaa cagcagcaat gatgttattc
      361 agcatggtag caagtacgac ttttgtttct gcaactgaag tatacccaaa ggaatataac
      421 acagaaggaa cgattacatt tgaagcagga gatgagggtg taactcctcc tgttgatcca
      481 gagaatcctg atcccaataa acctgtcgat ccatcagatc ctccaagccc tggaacagga
      541 ggggcattat cgattgatta tggatcaaaa ttcaaatttg ggactcaaaa aatttctact
      601 gcggataaaa cgtattatgc agctgctgat gtgatgaacg atgggtctag aaaaccgaca
      661 tatgtccaag tgacagatag gagaagtacc ttatctgggt ggaaattaag tgtctctcaa
      721 ccagaacagt ttaaaactgc cagtggggat gaactagttg gagcacagct gaaatttacg
      781 aaagggcagg ctgtttcttt ggttgatcca acatatacgc cgcaaacggt gaattctgaa
      841 ttaacgttga caccgggagg aaacaatacg ttagcaatta atgctaaatc aggaacaggt
      901 gtggggactt gggtctatcg atttggtgca aatgaaaatg aaaatcaaga tgcggtacag
      961 ctttcggtac ccggtaaatc tgtcaagtta gcgcaacaat attccactaa attagtttgg
     1021 acattagaag atacacctaa caactagttt actgaaaaga actgttaaaa ataaatacgt
     1081 agtcatattg tcttgcgagg taatcgagca aaaattgtca aaggctaggc taaatttgtt
     1141 tggatacgaa tagttcatct aggaatttaa cgatcaggag gaaaagatat attgaaaaag
     1201 aggaggatcg tgctcatttt aagcagtctc ttagtgacta atatctttcc gatcataggg
     1261 tactctttgg aattgaatca gttggagcat ggaaatagaa gagacgaaga aagcaaactc
     1321 acttcgacat tcgacagttt aatagaatcc aaccagacac aagaaagcag tgaggattta
     1381 agtatttcga gtagtggaga gaccactgac aacgtagaag ctgagggttc ggacaaacaa
     1441 ggactagaag atgcaccaat ttctgcggtt gagctgcctg acgatcccaa tattatttct
     1501 atcgataaaa tttttcaaac acctatcggt actagcacgt ccatattgga aaatgggaag
     1561 ttactgcagt tgaacccagc agaaaggtct caaagaggag ctatttggtc aaataagaag
     1621 attagtttac tctcggattt tacatttaaa tcttatttat acttaggaaa tcaacgagat
     1681 agagcgggag atggcatgac ctttacattg actggcgatc ctagaatggc tagtaatccc
     1741 agtcaagtta ttggatcgcc aggtatggga attggctcat attctactcg ttctggccaa
     1801 ccttacattg cgaatgcgct ctcgattgag tttgatacgt ataaaaatag tggttcttct
     1861 gatcgaatgg atcgagagat aactgctgat aatagagggc atggacatgt cgcttttgtc
     1921 acgcctaaac caaataataa taattattct ggagagcata ctggcgtgac ggtagctcct
     1981 acatttcttt ctaatggcac atggcgcttg ttaataatta aatgggacgc ggagcaaaga
     2041 agtcttactt atgatttgga aggtgtcggt cactcttctc ggattatacc tgatctcatt
     2101 aggcagtttg gttctacaga agtaaattgg gggtttactt cttcaacagg tacttttttt
     2161 caagaaaatg ctgtcgctgt cacgcgactt ccatcgaatg tcacatcaac agctgatgta
     2221 aaagtcaacg atagtgatta tggggccatc gctgaagctg gaggaaacga taagataact
     2281 atcagaaata cattgaaggt cgagggggat tttcttgaat ataagaaaat gcagactagt
     2341 atcgacctcc cacaagaatt aactattccc gagcatatct tgatcaatgg aaatcaaatt
     2401 gatgccaaag atatcttgat aaccggaaat cgagtcacgc taaatctgac agattatttt
     2461 attgctggag atacgttaat aatcgaattg gagacaactt tgcaggaagt aattcctgaa
     2521 aaagtactca ctatgcattt tgaatacctt gaaaacggga atttgattga gcaatcgaat
     2581 gaaataaacc taaagatcgc aaagctgaag gagatagata tccatgttta ttataaagat
     2641 aaagacagtc agattgatat tgctgaaatg aaaacaatca ctgggaaaat aggagagaag
     2701 tataaagaaa ctcctattga tattgaaggg tatgtttttg actcggattc agcgaataca
     2761 gaaggcatct tctccgaaaa tacagaggat atctattttt attacagaaa aggcaaactt
     2821 tatctctcaa ctgctcctca atatgtcaat tttggaaaac ataaaatatc cagtagacca
     2881 ttaagtgaat tcggacagtt ttcaggtggg ttgaaaatta tagatgaacg ggcttttgga
     2941 agttggaaat tacaattgaa acaagagaaa gttcttacaa atggagattt tgagcttcca
     3001 gaagctcttt cctttgttac ctctgaaaat acaacggtta tagggacatc cgctgttacg
     3061 attttcgaaa gtgatcaaaa gggtgaaagc gatttgtcct ctcttttaga ttctacagct
     3121 catcgtggaa tcagaataga tattcctgta gaaaaccaaa gaatcggaac ctttgagggg
     3181 aaactatctt ggatacttgc agatgtccca ggaaattaac aggaagagaa gaatataaca
     3241 aaattattga gtttcaagaa gggcacaggt accataggct cttgtgtccc acagagacaa
     3301 gattttgtta tagtcttctc ttacatatga ttaaaggagc gattttcttg agaccaaaac
     3361 aacaattatt tataacactc ctagttattt taggggcact ttttaacaat atatcagtgt
     3421 cagctagtgc atttaatttt tcagtgacgc cgataacttc agagaatcaa atcgacaaga
     3481 gaaaaactta cttcgatctt caacttgatc cggatcagga agtagaagta aaagcggaat
     3541 tgagaaatga tacagaaaaa gaagtgaaaa tcgatatttc tgtgaatagc gcaaccacca
     3601 attcaaatgt gatggttgaa tacggaaaaa acgagattga aaaagacatg agcctgatct
     3661 ttgatctggt agattatgtg agctatccac agatagtgac gctaaaacct aaaagtgtcc
     3721 aaaccgttgc ttttcatgtc aggatgccga atgagcgatt tgatggtgta ttggctggtg
     3781 ggatcacatt ccaagaaatc cagacggaaa aggaccaaac agaaactaag gatcaaagct
     3841 tatcgattga gaatgaatac acctatatta ttgctttgtt gatgcaacag acactgaacg
     3901 aagttgctcc gaaccttact cttcatgaag ttaaaccaga tcaaatcaat gcaagaaatg
     3961 tcatcttagc aaacgttcaa aatgatcaaa aaacctatat caatcaagta gttattgaaa
     4021 caaaaattac aaaaaaagga cattctgaag ttttatatca agaggaaaaa gaaggattgc
     4081 agattgcgcc gaatacaaat ttttcttttc caactgcttt aaatggtcaa ccgctaaccc
     4141 caggtgaata tcaccttaca atgactgttt taggaaatga aaatgaaaat ggaaaatttt
     4201 cgagaaaaaa aggaaatact acgatcaact ataccaatca atgggtattt gaaaaagatt
     4261 ttacaataga cggaaaagta gccaaggaat tgaacacgac ggatgttacg attaaaaaag
     4321 acaataattg gatttatatc ttagtagctt cacttttctt attgtttatt ttattatgca
     4381 tacttttgtt tgttttgaga aaaaaagaag agaaagaaaa gtaaggttaa gataacaaat
     4441 agtaaatttt tattattgta ggatggtt
//
LOCUS       DACDXE010000137         4425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.140, whole genome shotgun sequence.
ACCESSION   DACDXE010000137 DACDXE010000000
VERSION     DACDXE010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.140"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            690..1658
                     /locus_tag="IWM23_002843"
     CDS             690..1658
                     /locus_tag="IWM23_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAP9885421.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IWM23_002844"
     CDS             1669..2484
                     /locus_tag="IWM23_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAP9885422.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IWM23_002845"
     CDS             2552..3262
                     /locus_tag="IWM23_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAP9885423.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="IWM23_002846"
     CDS             3298..>4425
                     /locus_tag="IWM23_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9885424.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DACDXE010000138         4345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.141, whole genome shotgun sequence.
ACCESSION   DACDXE010000138 DACDXE010000000
VERSION     DACDXE010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.141"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(387..1853)
                     /locus_tag="IWM23_002847"
     CDS             complement(387..1853)
                     /locus_tag="IWM23_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAP9885425.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWM23_002848"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="IWM23_002848"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAP9885426.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DACDXE010000139         4312 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.142, whole genome shotgun sequence.
ACCESSION   DACDXE010000139 DACDXE010000000
VERSION     DACDXE010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.142"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..281)
                     /locus_tag="IWM23_002849"
     CDS             complement(<1..281)
                     /locus_tag="IWM23_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9885427.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEEN"
     gene            complement(730..1272)
                     /locus_tag="IWM23_002850"
     CDS             complement(730..1272)
                     /locus_tag="IWM23_002850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885428.1"
                     /translation="MKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGN
                     DFVDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADL
                     QVENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTL
                     FYRQNVSQRKLERSEDEIEW"
     gene            complement(2049..2239)
                     /locus_tag="IWM23_002851"
                     /pseudo
     CDS             complement(2049..2239)
                     /locus_tag="IWM23_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349771.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2359..2721)
                     /locus_tag="IWM23_002852"
     CDS             complement(2359..2721)
                     /locus_tag="IWM23_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885429.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2725..3033)
                     /locus_tag="IWM23_002853"
     CDS             complement(2725..3033)
                     /locus_tag="IWM23_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885430.1"
                     /translation="MDKIDLREETVDRIELMYERLADYLNLPDEDNYSEMLERIMDVL
                     NSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQISVINSKEKEM
                     "
     gene            complement(3042..3461)
                     /locus_tag="IWM23_002854"
     CDS             complement(3042..3461)
                     /locus_tag="IWM23_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885431.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3454..3687)
                     /locus_tag="IWM23_002855"
     CDS             complement(3454..3687)
                     /locus_tag="IWM23_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885432.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3684..>4312)
                     /locus_tag="IWM23_002856"
     CDS             complement(3684..>4312)
                     /locus_tag="IWM23_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HAP9885433.1"
                     /translation="SQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTWWRLELQ
                     LRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLAKNTKKK
                     YRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1221 a          959 c          618 g         1514 t
ORIGIN      
        1 atattctctt catgttggtc taacaactgc caaccattat tactaacaga tcttaaataa
       61 ccattacctt catttaggcg cataagctgt tcaaaattta aaatcaacac atcatttgga
      121 gtgtgataat aaatattttc tttcagtttt ccgactatgg taattcggtc aattgaccac
      181 tgcattttta agggaagggt caaactttga tactttaacc gcctgttaga gggggctgtt
      241 aatttttccc actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag
      301 aaaaagagac ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc
      361 aaaaaatctc tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg
      421 agctagtgcg attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt
      481 tttttgctct tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct
      541 atgcaaatat ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta
      601 acgctaggtt ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt
      661 agtggtttag ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc
      721 acatcgtaac taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt
      781 ttgacgataa aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat
      841 atccttttct gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt
      901 ttttaatcgc tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc
      961 cacttgtaaa tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc
     1021 tacatactca tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt
     1081 atcagcattt ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc
     1141 attcccagat aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat
     1201 atctttttgg aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc
     1261 ttcaatcttc actctctcag aagccaccag agccttttct cgctcatttt cttctctttg
     1321 cctagaaaaa tatacctcaa ctcatcagaa acgctctcac gctcttttac ggccgtttga
     1381 cgcaattctg aaatctcgct catgatttct ttatattcgt gaacatcttt gattttgtta
     1441 gtaccgacta actcacgctc ccaaccaaaa tctgccatca aacgttcaat tgactcaact
     1501 tcctgattcc taaaattatt ccaaatcact cgaccattct ctttatctgc tcgtaaaccc
     1561 tgctgttgca aggctttgtt aaaacttggt tgcttttgaa caccccgttt atacccttca
     1621 gcaacaggaa caatattcaa atgcaggtgc ggacttactt cgtcattatg aataacagca
     1681 ttgtagacgg ataaattagg attccgttct ttaaattctt tgaaatattc aactaaaatt
     1741 ttgttggcaa tcactcgatt ttgttctgct tgttgttccg atatgccagt agaagaacca
     1801 tcatcacgag tagtacgaaa atcatcaaga gtaccaattt gcacgataaa ttctcgctgt
     1861 ggttccaatt ttttatcctt gcgaaccttt gctaaatagt cctcgatttt tctatcggct
     1921 cttttctgct ttgcattata tttttctacg gcttcaccga ataattcttc atatttttca
     1981 cgaatatttg tttgttctaa atagatattt tcatccgatt ttgtgaaatc aatatgctta
     2041 tgccagtcat tgattttttg gctatcagtt aactcacgat tattatgaga aatgcttgta
     2101 ttctttgttc cctttttgaa cgaaatactg aaccccaagt taaaaccctc cttcggctgt
     2161 cgctaataaa aagagtgtaa acctttctgt tactctttgt caaagagtaa cattttccta
     2221 attgtgacat ggctgtcaca attccaaatg ctctccgagg gtattcgctg tcacaagtga
     2281 caacgaaaaa aggagccgaa agcgtgtcac gctctcaact ccttttacgc tttattttgc
     2341 ttcacgcaaa ataatcgctt atcgtaaatt cacgtcatta aatattgacg taaatttact
     2401 ctcactcatc tgacttttct tattcggtat cactccgaat aagccgtcag attcatcggg
     2461 ataatctcgt tcgcttggcg ctgatttttc atcagcaagc cactcattat ccactgattt
     2521 ttcgtgttgc tgatcgtcag aaatcgacga tagccaccgc tcaaaatcag caaaagtttc
     2581 aaacccaaaa cgcttccgaa gagccttgcc cattgcagca ttttctttct caatcaagcg
     2641 ttttttctgt tggcgaagtt tcgcttgttc tttttttaat ttttcttctt gcgctttaat
     2701 atctgctaaa gtcattccca taaactacat ctccttttct ttggaattaa taacggaaat
     2761 ttggtttaac tgcataacat gactcattaa tttcaaccaa aattcttttt ctgattcatc
     2821 agttgtttgc gctaaatgct tatgaacaaa ttcattagtc aaaactgaag attttgctaa
     2881 atcagcattg ataaccgagt tgagaacatc cataattcgt tcaagcattt cactataatt
     2941 atcttcatcc ggcaaattca aataatcagc taaccgttca tacatcaatt caattctatc
     3001 gactgtttct tctctcaaat caattttatc catgtcacac ctcacttcca gaattgccac
     3061 cattttcgat tttttaattc ttgattttct agtaacaact gatcgttttt ttgttgctga
     3121 agcagggtca atttttgttg atcacgaatc atttctgtta attcagaaat atgtttgtcc
     3181 ttctcagcta attgatcacg caaaaactca ttctcttttt ctaaaggaaa atcactgcct
     3241 gtgttattga ccggtaattg accagtcaat tgatcacttt ttgaccgata aattgaccga
     3301 acaacaccaa tcaactcttt atctagcatg ataatgccat tttctttata ctgagcaagg
     3361 tcaaaccctg aattgaccag ttgattgcgc actgtatttt tcgatacacc caattcgtca
     3421 gcaagatttt ttatggtcat cttttctgac aaatcagtca ttagcttctc cactactttc
     3481 agctaccgca taaatatatt taacggcgtg cttagggata ataatcggag agtcaatttt
     3541 attatttttc aaaatcaatt catattgcgt tacatcaagt aacttaccgg acattttttt
     3601 gctatccaaa aaaacaacca caacgtaatc acctgttaaa tttaccaacc aatttttctt
     3661 accaaaatac ttttgttttt ctgtcatttt tcttcctcct ctgttacgtc tagccctaga
     3721 agccatgtgt ctaattcttt ttttaaatcg tctgccgatt ccgcaaaggt ttctcgcaaa
     3781 tgattcgtca attcatcatt ttgactttct tgcttcaaca aatccctgta ttttttcttt
     3841 gtattcttag ctagatttcc ccactcagaa tgatcataaa gcaaaccttt caccataatc
     3901 cgttcagtaa ctttagtatt tggcggaaaa taatgtggac tagcaaagct atccaaactt
     3961 tcatgaacca tctcatgcca atcagttgct tttccccttc gtaactgcaa ttctagtcgc
     4021 caccatgtcg ttatttccgg cggaacaatt ttacgttttc tttcttgttc cagtttttta
     4081 ttatacatcc gaatttgtcg ttcactggca cgactacccc aatatgccgt ttctaactta
     4141 ccagaacgcc cataaattgg tttaaaagac acaggatcaa ctacccgata ttgtgtaata
     4201 aactcatcag gtacatcgat aatgtcgcaa gcaatatccg cacgtgaaaa gtgaggttcg
     4261 ataaacaaat catgaataaa atttttcatg gaactagcta agaattgact aa
//
LOCUS       DACDXE010000140         4307 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.143, whole genome shotgun sequence.
ACCESSION   DACDXE010000140 DACDXE010000000
VERSION     DACDXE010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.143"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWM23_002857"
     CDS             complement(<1..538)
                     /gene="sgrA"
                     /locus_tag="IWM23_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAP9885434.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTE"
     gene            complement(734..1573)
                     /locus_tag="IWM23_002858"
     CDS             complement(734..1573)
                     /locus_tag="IWM23_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAP9885435.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            1758..2645
                     /locus_tag="IWM23_002859"
     CDS             1758..2645
                     /locus_tag="IWM23_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAP9885436.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(3229..3924)
                     /locus_tag="IWM23_002860"
     CDS             complement(3229..3924)
                     /locus_tag="IWM23_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAP9885437.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(3908..4306)
                     /locus_tag="IWM23_002861"
     CDS             complement(3908..4306)
                     /locus_tag="IWM23_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAP9885438.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1425 a          854 c          639 g         1389 t
ORIGIN      
        1 tttctgtgct actttcactg ctcgtactct ctgtactgct ttcaccgctc gtgttttctg
       61 tactgctttc actacttgtg ctatccgtac tgcttggcgg tccaacaact gtccaactgc
      121 ttggtttcaa attctccgtg atcgtcatct gtccatcata aaaaacaatc aaatcaactt
      181 tatctttact cttgatctca tcaatcgata gttctgcaga aatcattatt gtatactcaa
      241 taccagcggt ccattgtacg tcttttaaat aaacttcccc aataactttt tctgaacttc
      301 cattaccatc ccaaccatat gttagaacag catgtacatc actagcattc acgcttttgt
      361 ttggtttgac tggaatagta atgacttgtt gtgtcatgtc ataaacaatc cgatcagttt
      421 tcacagtaaa tgaaagattc tcatttgccc gttcatttgc agaagtactg attccaatca
      481 ttgctgcaat tatactcagc ccaagaataa caaataatac ggtcgctgtt ttcttcattt
      541 ctatcactct ccatataatt accttctatt tgataaagga attatataaa atgaaaatga
      601 tgagaacacg acagtcagtt gtgaaaatct aacaaaagat gtcgtgtatt cgcttgaatt
      661 tgtttacttt ttcgtttaat caatgacaga cttaaaaaag caagaccgat atcagccttg
      721 ctttcttcat cttttattga taaacagcag tgtatttctt catccgcttt gttttatatg
      781 catagagtaa caaaccaaca atcaagacta acaagaataa tgtagttgat atagatgctt
      841 cagccccaaa gtttccccca gtaattattg tattttgcga ttggatcaga gtggagaaaa
      901 aaccttgttc ttgcgttccg ctgactggta tacccaaaat agctccttgt gtccaattcc
      961 aagtaatatg agcaccaata gccgtaaata aacttcctgt actgtagata agtgcgctga
     1021 aaaagcaacc tgctaaaaag atattgaaaa ctgccagaaa tgtcatgtta ggattcaatc
     1081 cgtgcaaaag agcaaataat gcagcagatc caatcattgc tattaacgga ccaaatctaa
     1141 gcatctgatg ctgaaaaaag ccacggaata aaagctcttc tgatgccccc actgcaataa
     1201 ataaaataaa actcgttatc agcggcacaa tgtttgcgtt tcctgaatct aaccgatacg
     1261 cccccgtcaa atataatgga ctagtaataa gcagaaacac tccaattcca acaacgagac
     1321 ctcccgctaa atacccccag tcttttcctc gaacagaaac accataatct tctattcttt
     1381 ttccttcgac cttactagca agaaataaag ctagtaaaaa aacagctaaa tgtccgaccg
     1441 taccggcaat tgctccaatc cattcactat ttccaataaa catatccgca agtgcattat
     1501 tgacaagaat tcctaaaaca aaaccaacta agactattaa aacagatcca atcaaccgaa
     1561 taatcctgat catctttcat ccctccttta tattaagttt aacaaccctt ttctttcttg
     1621 tctcgtgaaa actatgtaaa tgttctccat catagagaga atagattttt acgaactaag
     1681 aatatctaca gcaaccttac gaaaatgaaa gactgtctcc ctaattcttc tgttagactg
     1741 tttttaataa ggaaaggatg ggaaattttt tgaaaaaaca actactaatt atgtgccttt
     1801 cagcatgtgc aattttacta ttctcaggtt gtagcaagtc caatgaaacg attgcaacgt
     1861 ttaaaggtgg aacacttaca gttcaggatt tttatgataa cccaaaagtt aaatcaaata
     1921 atcaaacact attaaaaaaa atgattgtct tcaaagcatt tgatgatgta tacggaaaag
     1981 agatttcttc aaaggaagta acaaaagaat ataatgaaca aatcaaaaaa ctcggtccaa
     2041 actacaagga acaattaaaa gcagttggcc aaacagaaga gacatataag aaactcttta
     2101 aacagatgct ggctttccaa tacggtttga aagccaatgt aaaattaaca gataaagatc
     2161 tagacactgc ttggaaagaa ttctatccag aagttagtac acagatcatt ctattttcga
     2221 ctaaagaaga agcagataaa gcaaaaaaag aagcaaatga aggggaaaat ttctccaagc
     2281 ttgttcaagc ttatggaaaa aataaaacat taaaggaaac tgatggaaaa atgaattttg
     2341 attcaaccaa tccagagatt cctactgaag taaaaaaagc agcatttaaa ctaaaaaacg
     2401 gggaagtttc tgatatcatt cctgtaacag atccaacgac ttatcagcaa agctattatc
     2461 tagtaaaaat ggtaaaaaaa caggataaag gttctaataa agacaaatac aaatctgagc
     2521 tagaaaaaat tgctaccgaa gcaaaaactc ttgatacgga attcatggat aaaacaatcc
     2581 gtaaagtgat gaaaaaagac aatgtaacta ttaaagatcc ttatgtaaaa aatatattca
     2641 aataaacaaa tttgacccaa ttggggatgt ggcaaaattt gagaagtgat ttttggaaat
     2701 tccttcttat ttttgaggca aaccactttt gccacaactt catcggggcc tttttttatt
     2761 tgtaccaaaa caaatcactg gatttgttac aatcatgagg atataattta aaaaagtttt
     2821 ttatttctat cacatgaatc aatgcgccat taagtagatt ctttcatgct tccctatttt
     2881 ttcacttgcc gtatcgcttc aaaaacaact atcataaact caaactcgac tgaaaagtta
     2941 ttttaaaaaa gactaaaaat atcccttctg gacttgttcc tcttattctg ttatactaga
     3001 gaaacacgaa agatattttt atgcgacact tttcatttgt ttttatgaaa tttattcaaa
     3061 actggcgtaa cttcagagtt acaaggatgt attagaggtt agggaataca tcgtttaaat
     3121 gagaaataag taatagtcac ccaaacttta aattaatgaa tattgaatcg tcagatagcc
     3181 tcctcgagtt ttcgagaagg ctatcgttta ttttatggag aaattgattc agaagtaatc
     3241 agcgaccttt tttagttcac caatcgtttc tgcttggaaa gtttgcaccc aatcttcttg
     3301 gaagcccctc actgctttat caatcgaggg catctctaat acttgatcta gtagctcttt
     3361 acctaacgtt atcgcatgag ctccagatcg aatggctgta gtgacttgtt cacaattttt
     3421 gaaactagct gctaatattt ttgttggtct ttgcgtacgt tcgatctcag tccgcaacga
     3481 atcaatcact ttcatagcat caatgccttg attttgcatc ctgttataat aaggtgcaat
     3541 ataatctgct ccattattaa tagctaaata cccttgtaat tctgtgtaaa tagctgtacc
     3601 ggtaatcaaa taaccttctt ttttcaactt tttgatcgct tgaaggcctg cttgattcac
     3661 cggcaccttg ataaacgtat cttttcccag ttcttctatg ataactcgag catcctctat
     3721 catttcttct tccgttcttc ctattgtctg aatatggata ggaatctgtc cgattatttt
     3781 tttgatttct ttcatatgcg agaagaagtt tgacgctcct tcttttttca gaattgtagg
     3841 atttgttgtt acgcctgtta aattcaaaaa ctgttgacaa gaataaatac tttttgtatt
     3901 tgctgtatca agcatgattt ccatatagct ttcccccatt ctgatcctct tgatcgattt
     3961 taatgattgt cccctttctt aaatgcggca tttccttatc ttctacatac attgttcctg
     4021 gaagttccgg ctcatgagat ccatcaaatt ttatcgtgat atgtcctaat tcattcaaat
     4081 tagtttggac aacatctcct actgctgtga ttacatacat ttgtttatca aaacagagct
     4141 tcatatcaca agttatttct tttcttacct gattcaattc aatcttataa caataatctg
     4201 ttaatgtttc cggtgcttca tctccaaata aaatcattat tttttctttc ctgaatactt
     4261 ctgcttctat accaacatct gttacaaccg tttcaaagat cgtcatc
//
LOCUS       DACDXE010000141         4219 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.144, whole genome shotgun sequence.
ACCESSION   DACDXE010000141 DACDXE010000000
VERSION     DACDXE010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.144"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..1257)
                     /locus_tag="IWM23_002862"
     CDS             complement(37..1257)
                     /locus_tag="IWM23_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9885439.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IWM23_002863"
     CDS             complement(1277..1882)
                     /locus_tag="IWM23_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAP9885440.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IWM23_002864"
     CDS             complement(1931..2863)
                     /locus_tag="IWM23_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAP9885441.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IWM23_002865"
     CDS             complement(3284..4144)
                     /locus_tag="IWM23_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885442.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1198 a          853 c          690 g         1478 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgctt
//
LOCUS       DACDXE010000142         4153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.145, whole genome shotgun sequence.
ACCESSION   DACDXE010000142 DACDXE010000000
VERSION     DACDXE010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.145"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..106
                     /locus_tag="IWM23_002866"
     CDS             <1..106
                     /locus_tag="IWM23_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885443.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            970..1182
                     /locus_tag="IWM23_002867"
     CDS             970..1182
                     /locus_tag="IWM23_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885444.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="IWM23_002868"
     CDS             complement(1460..3259)
                     /locus_tag="IWM23_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAP9885445.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="IWM23_002869"
     CDS             complement(3383..3979)
                     /locus_tag="IWM23_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAP9885446.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DACDXE010000143         3923 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.146, whole genome shotgun sequence.
ACCESSION   DACDXE010000143 DACDXE010000000
VERSION     DACDXE010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.146"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(573..1130)
                     /locus_tag="IWM23_002870"
     CDS             complement(573..1130)
                     /locus_tag="IWM23_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885447.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(1309..1920)
                     /locus_tag="IWM23_002871"
     CDS             complement(1309..1920)
                     /locus_tag="IWM23_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885448.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(2033..2290)
                     /locus_tag="IWM23_002872"
     CDS             complement(2033..2290)
                     /locus_tag="IWM23_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885449.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(2887..3165)
                     /locus_tag="IWM23_002873"
     CDS             complement(2887..3165)
                     /locus_tag="IWM23_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885450.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(3199..3450)
                     /locus_tag="IWM23_002874"
     CDS             complement(3199..3450)
                     /locus_tag="IWM23_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885451.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         1194 a          799 c          520 g         1410 t
ORIGIN      
        1 ttattccgaa taaatggtgt ccaaaagctt gcatctgcca gattatctca tttcttatga
       61 caagctaagc aaattatctc caccaaataa tttattcata tttttgactt gcgcatacta
      121 actaattctt aaattttcat acaaaatttc tcaatttccc ttaacgagta atatttatgt
      181 acggtgattc tcactagtat cccaatcact ttttaagtta tcccgatttc aattctactt
      241 caaaaatagt tgcttttatg tatgctgtta gttgattaga tggtcatttg ttgagctagg
      301 tcgtttattt taaacaagtt gtttctttag cttataccta tctcgtctac caaacgcaag
      361 atggcaaaca caaatgcata cgcatctgta tttgccactt tgattcgtat aaagctaaca
      421 actattaact cctctcagct aatagttgct ctctttttta ttcagattca tttttgctga
      481 tcacagccta gtatttcaat ggttttgaac actaaaccaa attgttccat tacaacattc
      541 actggctgat gaaatggatt agtagatttc gattattcaa agcgattttc tattggtgga
      601 tcgtgttccc aactaataag acggattcca aacctatcgt acagatcttc taaaccactt
      661 ataaattttg cacctatttc aggtgtttta ttgcgtggat attctattgt tacgccatta
      721 aatggatacc aattaggatt aggctctctt tttttctgta catctcccat agaatagtta
      781 gcactttctg ggacataata tattcttcga ccatcctcta attcatctaa taagatattt
      841 cgtgatcgtc tatcacttgc taaatgtaga cgccacgtgc gataccatgc catgctacct
      901 gcatctgcag aaaatccatc aaataatgca acaattgtat cttctatcac aatcctttcc
      961 ccatctactt catattctaa tctaaaatca aactcccctc gggtaatttc tggtcttggt
     1021 ggcttaggac ccaatattat tctacttaaa accgcattcg ttcccagatg aaacacagct
     1081 ataacaacaa taatgattaa tattttgttt ctatttgata aatttctcat tattcgcaca
     1141 tcacctctac attctcacat attagttcgt aatactcctt taatatcaaa atatctactg
     1201 gtcgtaataa tattcgctat gaattcctac tactcgtata agaaaggcca aatcatcagc
     1261 ttttacttgc tgatgatttg gcttgtttct atttatcgta attgttttct aatcaccaaa
     1321 attgttaaca atcggttcag atatttccca gttgattaag gtaattccgt attgtgtcaa
     1381 aacttcttgc gcttctccga aattaagtac agttcttatg tatccacttt ctcctaatat
     1441 aaagtctacc tctcttttaa aaccttcctc gagatttcta agggctatat gtggttttaa
     1501 tgacattgat tgtacatttt cccccatata atattgcgca cttcctcgac taaaatagat
     1561 tcctagatcg tccccaaaag gtagatcttc tctaccacta gctaatctac ttctccatct
     1621 tcgttctctt ttcatagaac ctgcgctgaa ctcacacccg ttaaactcag ctatgacagt
     1681 atcttcaatt atgttaatct gtccatctag ttcgtattca atcctaaatg gaaattctcc
     1741 ataggtgatt tccggttcgg gtattttagg tgagattaat gcaaagaaag gaaatattaa
     1801 gcttggcaac atcaaaaaaa ctcctcctat gatcacaatt cccgatgtaa tccaaaatat
     1861 aatttgtaca attctcattg atccattgta ttccatttta ttccccctat tactattcat
     1921 atccgcctat ctatatcgat cttccaatgc agagataatt gcttccatac cttgattttt
     1981 tactgagtta cctatgcgag atatcattct ccactaaggt gcatcatatt gtctatctaa
     2041 catgattaat tctccaatag gtgtatactc ctccaaaaac aacatttaat gcatctccct
     2101 cgacaacata agctaccatg tttgctgtat atgtgtttga gtctaattca taagcagata
     2161 aatttactgt cgctggatta aatataatcg cattcgtatt tatagctact gaatttgctg
     2221 cttcagcacc accttttgaa tgtccgatca ttgtgatttc aatagtagga tttacgtgga
     2281 cagccctcac cgtttaccct gaccattata gaaatcgctt acttttttga actacgcaac
     2341 aaaggataca atgctcctaa cttcactgtc tccatttttg tccactaagg aatataaaaa
     2401 ataggcatta attcatttaa ttcggtcaca acacgttacc ttttaaatca ttatactgta
     2461 tctcatacat tattatcaat aaactattat aaaaatgcaa aaaatgcttt atcaatctat
     2521 tttgtaattg gtcataatat cattgaaatc ataccctgta tcgatttttc ctatgaatct
     2581 cgtctatttg tgattgacga aaaagaggca agacagacac tggaatggta attttaccca
     2641 ctattttgct ccaactattt gtaatcgtac ccacttttga aataccgaga tgatcatttt
     2701 aattgatatg ctgattccca agcaaaaccc caagaaatta aatgctctat cgcatctagc
     2761 ttcttgggtt ttgcttatat aaattccaac atttaaattt tttatgctgt tcggtctcag
     2821 aaatgccgtt tttgcttata tcacaatatc aagatgtcaa attattcgtg ttgccgatga
     2881 ctttgattaa tttttttcaa attgtttatt aagactaatg ctgtcttttt gcagatacca
     2941 atgttcgaac tttttaacat tatcatatga tcctgcaagt tttaacttct taccattaga
     3001 tgttatcaaa gttataatta accttgcctt tttcccatta ggatctttta ttttatgaat
     3061 tttctgcagg taatctctcc taatggcagc tatttttacc tcatcagtgc tgataaacca
     3121 ttgatcatcc acatacatac tgcctgttat gttgtgtttt tccatttctt ccgagaacga
     3181 aaagtttaag gctcgttctt acttccaaag ttgaatgagt ccatggattg gacttagtgt
     3241 gagaattgag aacaacaata agaatccata tcctcccgca aacatcccta aggctatact
     3301 gtctctccat ccataagtag gattagaaga gaaaagagtc accgtttgta ataatatgaa
     3361 aaggaataat ccagcacaaa caccaaagat aacaactcca aacattgaat ttttatacaa
     3421 tgaccattta tttataggct tttctttcat tttacttccc cctactgttc tgttcacacg
     3481 gaatgaattc aaagttcata agccctcctc tttgaacata tgaatacgac ttgctaacta
     3541 tactttaaaa aagcttgttt taagcggtta taggcgagtt gtaatctcta ttcatctcat
     3601 tagcaaataa cttgccgaaa agtctactga aattaaagca gatagtggaa gaattattta
     3661 gccaggatag acactatttg tgaaacacat gtctttactt ttgaaattct atgatgaaaa
     3721 gccttggaat acagtagctc ttaattggta atactttttc cattataaat aacctctttt
     3781 cattttatat ttatcccctc taaaaataat atctaatcta atattttaaa cttatatttc
     3841 cctcgctgtg ctaatagagc attactaaat aaatagaaac tagaaaaaag cattacgcct
     3901 gccagaataa accaattatt tgt
//
LOCUS       DACDXE010000144         3712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.147, whole genome shotgun sequence.
ACCESSION   DACDXE010000144 DACDXE010000000
VERSION     DACDXE010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.147"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWM23_002875"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IWM23_002875"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAP9885452.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IWM23_002876"
     CDS             complement(2260..2511)
                     /locus_tag="IWM23_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAP9885453.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IWM23_002877"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IWM23_002877"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAP9885454.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IWM23_002878"
     CDS             3461..3679
                     /locus_tag="IWM23_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885455.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1025 a          796 c          688 g         1203 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca ag
//
LOCUS       DACDXE010000145         3606 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.148, whole genome shotgun sequence.
ACCESSION   DACDXE010000145 DACDXE010000000
VERSION     DACDXE010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.148"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..963
                     /locus_tag="IWM23_002879"
     CDS             4..963
                     /locus_tag="IWM23_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9885456.1"
                     /translation="MNGFAYTINNEFNLGANEGSSQVANNQYILLHETANETATGRNE
                     AQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGSYANANSPVQIELQHTHDKAT
                     FEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHLWVTQNIWGDHTDPYGYLSEM
                     GVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLSDGTNHSHIDQFGEIEN
                     ANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYHVSF
                     NMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1152..1225
                     /locus_tag="IWM23_002880"
     tRNA            1152..1225
                     /locus_tag="IWM23_002880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1186..1188,aa:Ile,seq:cat)
     gene            complement(1590..1796)
                     /locus_tag="IWM23_002881"
                     /pseudo
     CDS             complement(1590..1796)
                     /locus_tag="IWM23_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2140..2547
                     /locus_tag="IWM23_002882"
     CDS             2140..2547
                     /locus_tag="IWM23_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885457.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            2561..2962
                     /locus_tag="IWM23_002883"
     CDS             2561..2962
                     /locus_tag="IWM23_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885458.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            2964..3335
                     /locus_tag="IWM23_002884"
     CDS             2964..3335
                     /locus_tag="IWM23_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885459.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(3371..>3606)
                     /locus_tag="IWM23_002885"
     CDS             complement(3371..>3606)
                     /locus_tag="IWM23_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885460.1"
                     /translation="YKVWNSEVFFLAIEDSLLINLYYSNKNYFKIPAAKTRMKKGVYF
                     LFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         1264 a          564 c          643 g         1135 t
ORIGIN      
        1 ccaattaacg gatttgccta tactatcaac aatgaattta atttgggcgc aaatgaaggt
       61 agctcacaag tagcaaataa tcagtatatt ttactgcatg aaacggctaa tgaaacagca
      121 acaggacgca atgaagcgca gtatatgcaa cgttcatgga ctagcgctta tactgcttat
      181 attgtgggag acggcggaat tgtttaccaa gtcggacaac ctggttatgt acagtacggt
      241 gctggttcat atgctaatgc caatagtcct gtgcagattg agttacaaca cacacatgat
      301 aaagcaactt ttgaaaaaaa ctataaagca tacgttgaat tggctagaga ttcagcaatg
      361 aaatatggta ttccattaac attagacacg ccttataacc aaccaggaat caaatcacat
      421 ttatgggtaa ctcaaaacat ttggggcgat cacactgatc cgtatggata cttatcagaa
      481 atgggtgtaa gtaaggaaaa actggcttac gatttagctc atggatttac agacgaaaac
      541 ccaacgactt ctgagaacaa gcctgtcatt gatccaaccc gagcaggtgc agcaaatcca
      601 actttatcag atggaacgaa ccattctcac attgatcagt ttggagaaat cgaaaatgcg
      661 aacttacacg tcgctggatg gcacatcgct aactataagt acgagtatat tttcattatg
      721 gactacaata ctgggaaaga attagctcga gtaagagctg atggaattta tagaccggat
      781 gtaaatcaag cttataatac ttctggaaat gttggctatc atgtatcttt taacatgcgt
      841 aacttcccta ataagaaagt ctatgtcatg atgcgggcaa cgaatgatcc agagggaaac
      901 actaaaggcg gtgcgcaaga tttccatgac aaacgttggt atttaaatat tcctaaacga
      961 taaaaatagc tcctcgttga ggagcattac ataactatat tgacaactat aaaaatcatt
     1021 cgataaaata gtgatgttat cgcatatctt cactatcacc cataatagtc acactccaag
     1081 ctatgcgata acaggtttgt tgccacacat tctactggtt gattgtttat ggctttatgt
     1141 ggcaacaacc agtaccctta gctcagttgg tcagagcaga cggctcataa ccgtccggtc
     1201 gtaggttcga gtcctacagg gtacattaac gtagccattt gaatcgttct gtgttagaat
     1261 tttttgaaga gtattataca agctaaagct tttcttcatt gccactcaaa tgagtggctt
     1321 ttttatgtat ccttttatgg attaatgaaa ggatgtttca catagttata tttctgtata
     1381 tttgaaaagt tttactttga tttttaaata gaaagacatt tgggttatat tgtgagataa
     1441 taataaagaa gagtttaaag cgttccccaa aaaccactgc cccataagtg tgttacgctt
     1501 taaactcttt tatatttgaa gccattaaaa agcataccat atttttgaaa aaaagtgaga
     1561 aaaaaggctt ataattggag tggtagttat tctataacat caagtttaaa ttttgtagtg
     1621 tagcttttgc cacctccaac caagccttcc caaccatgat agttataaat ccttacccaa
     1681 tgtctaacca gtgtacgatt tatttgatat ttatgtgcaa gatatttgta gccgccttcg
     1741 ttatttaaat agtctgaaac tacttttttc ttaaaaacaa atgtatattt ccgcaaaaaa
     1801 agcacccctt ttaattagat ttctagtcta acttttgggg tgcacatcat ttttgagggg
     1861 aagtacatat agttgttgat atagttgcta atattgttag ctaatttgtt agtaaaactg
     1921 tatacgaatt tagatagtaa aaaaacagtt tgttccttat atagaggcac ttttactact
     1981 gattaaatgt cagatatgct tacaaatata cttataaata tgcttacaaa attagctaat
     2041 atttccgttg acaaagcttt gttactattg ttaaactaaa ttgactagca aaagtgaaac
     2101 ataaatttat taaataaatg taaaaattag gagaaatcaa tgtcttatag agtacaatta
     2161 ataatttcag aagatgtaga aagtcagcaa tttggcacta atgttattaa taaagtaatt
     2221 aatccagctc tttctattaa tgcgccttta attcctactg ctttatcatt ttctgtaaca
     2281 gctgtggtgt ctgagataga ggatacagaa aagataaaaa tagtagaaat tgaagtttta
     2341 aacaagaatg aaaaacaaat tttttctacg ggtgaagtat cggtaagctt gcctcctcaa
     2401 gttaacgata taaactttaa tataaacgcc agaaatgtct tggttgaaga agcaggagag
     2461 cattatgctg ttgttaaatt taatggaact gagattggaa ggcaaatctt tgatatcaag
     2521 gtcaacaaac cagtggaaaa aaattaagga gacgatacgg atgcttgata tagtaccatc
     2581 aaatacacat cggagtggta gcttaataaa ttttgctgcg atttttgctt gcgcatcggt
     2641 aattctagca tctccagttg ttgtggatgc aagttctact ccgaaaacta aaaatgataa
     2701 tcaacttgta attactacaa aaaatgagat taacacaatt tcaagtaatg atggcaaaat
     2761 ttttgatgtg ataaatacgg ttttaaaaag aaaagctgaa atcaatataa aatatgatga
     2821 agatttaaac ttgtatttct ttgttatcaa aacaacatct gaattgtttt cctcagatta
     2881 cgatgttttg gatactctcg ataatgtatt atctgactat aaatatatgg ggaaatctgt
     2941 agtagcaact ttgggggaat aaaatgtttg actttaatga atatttatct atttccaaac
     3001 aaattgaatc agatgataaa tataattcta aagaatctta tagaagaact gctgtttcaa
     3061 gagcatacta cagtgctttt aaaaaatctg atgagtattt gaaagaaaat tatgatatta
     3121 tttataatgg aagtagcgga aaaggaagcc accaaaccgt ttggaatttg ttttcaacag
     3181 ttaaggaatt gaatacctta ggaatacata atagtgggta tagaatgtta gagaaaagaa
     3241 aatgtgctga ctatattcct aatgaaacca ttacgaaaac agatatggcg ctgatgaatc
     3301 gagaagcaga aaaaataata aacaaactca cctaatgaat ttcaagcgag tttgtttgtt
     3361 atttttttat ttaatctttg tatctttctg gatcaacgaa agtatacttt atatagtcat
     3421 aacgccgatg atcgctacgt gcgtccggca cgtcagtcac gatatcaaac aaaaagtata
     3481 cacccttctt cattctagtt ttcgcagcag gaattttgaa atagttctta ttagaatagt
     3541 agagattgat taataagcta tcttcgattg ctaaaaagaa aacttctgaa ttccatactt
     3601 tataaa
//
LOCUS       DACDXE010000146         3339 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.151, whole genome shotgun sequence.
ACCESSION   DACDXE010000146 DACDXE010000000
VERSION     DACDXE010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.151"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            106..414
                     /locus_tag="IWM23_002886"
     CDS             106..414
                     /locus_tag="IWM23_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAP9885461.1"
                     /translation="MFQNKGQRYVTKGVMDSLPVELQALCWNLIDQNVQKQFHLDYLQ
                     IFEFSTEKGNQKLVHRQEEPEARKEYLISPKLRLKSVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            441..740
                     /locus_tag="IWM23_002887"
     CDS             441..740
                     /locus_tag="IWM23_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HAP9885462.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGMLPEDVT
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVRGLLVEGRLVWSQIGGTNE"
     gene            914..1465
                     /locus_tag="IWM23_002888"
     CDS             914..1465
                     /locus_tag="IWM23_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HAP9885463.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEAEDKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFPRGNDEQSLE
                     ILTTVFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLADSQLLFQEK"
     gene            1611..1904
                     /locus_tag="IWM23_002889"
     CDS             1611..1904
                     /locus_tag="IWM23_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885464.1"
                     /translation="MEIDETCLSNSYKSMNDFFEEMELDSELLYQFYLAFRGQQISFP
                     MKMYDRELVRKRIENMIEQEKTVDIRQLTEVYGFSARWIQKVIKQKERRRVHG"
     gene            1897..2193
                     /locus_tag="IWM23_002890"
     CDS             1897..2193
                     /locus_tag="IWM23_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885465.1"
                     /translation="MAKLIVLGGLIYGGYWLYKYMLAHPNIDKGKVIKTVGVIIVGLI
                     ALTKKNNTNSPKIMRTYRCRGCGREISTSFNPSESPQGRDRCHAGYRHSWERIN"
     gene            2264..3268
                     /locus_tag="IWM23_002891"
     CDS             2264..3268
                     /locus_tag="IWM23_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAP9885466.1"
                     /translation="MKKWLLLLIGMGILFTGCTSENTRTKETVETDTTIEAKKSKTQS
                     TEQTTTEMKQSVEASTSESSIIESSSEEVKNTLWDSEKSTQLAIFVPQWGKTLGQEYK
                     SYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGHADFNLVAVYSDVET
                     GEYLGQHVYFFGFQNGQPKVYLTQQNQGNDKNYMYFNETQNQQLKQGFIDIVNGQTPQ
                     TPVVEESTQQATTTTTQSTSSSAISSWEAAKETVLNNKALWLYDSPDKNASNTNLIST
                     NEYMKDLMQDENGSYYSINIVTDDNGNTTMAGGKQFRVYTDGRISQRIGMTNNFQMIK
                     "
BASE COUNT         1260 a          460 c          658 g          961 t
ORIGIN      
        1 gtataaaatg aatcaaattg aatatcaaat atatattata tccatgaagc ccaatcttct
       61 ttaatagaag attgggctat ttttatggca aaggagggaa aagaaatgtt tcaaaataaa
      121 ggacaacgtt atgtcacaaa gggagtaatg gacagtttgc cggtggaatt acaagcttta
      181 tgttggaatc taatcgatca aaacgttcaa aagcaatttc atttagacta tcttcaaatc
      241 tttgaatttt ctacggaaaa aggaaatcaa aagttggttc atcgtcaaga agaaccagaa
      301 gcaagaaaag agtatctgat ttcacctaag cttcgtttga aatcagtgtc tcaaaaaata
      361 tgggtcattg attcgggtga gtatcaaacg atgttattac cagaagaata ctgatatcta
      421 aaagaaagga acgaaaggac ttgttagaaa cggctatcga agtactggaa aagtgtgcac
      481 agctagtcac tactcctgaa gagtggggct atgaatccgt tacgatggaa aaagaggaaa
      541 ttgaaatggg tatgcttcct gaagatgtta ctctaccacg tttagtgatg acacatcttt
      601 atatttattg cgcaccagaa gatggaaaag actatgtagt ttatttcatt acagatatta
      661 ccagccagcg agaatttgtt cgtgggttac tggtagaagg acgattggtt tggagtcaga
      721 ttggaggaac gaatgaataa gatcgattat ttgagaatga ttgcgaaatg tttagaagat
      781 atccttacag cgattcatca aattctgaag aaaaaaggag aaagaatatg aaatattttc
      841 aactagtatt gacacttata acggcaatgg tgattgaatt gcttgaacaa aaattagcat
      901 aggagaaaaa gagatgattg aaaaagaatt agtgaccaca caaacagaag tttggcgaga
      961 tcctcatcat acccatcgct atgttttcaa acgacaatgg gcgatcaaag gaaaagaagc
     1021 ggaagataaa ttagcggtag tcattactat tcgtccaaca gatacagaac catttacgaa
     1081 tgatttgagt atgttattaa tcgaaaagaa tatccgacaa ttaggattta ctggttttat
     1141 tgctgtgaat cttttttcgt atacaaaagc gaaaagtcct cgggtatttc ctcgtggaaa
     1201 tgatgaacaa tctttggaaa tattaacaac tgttttcaca gaaaagaaaa tcaacaccat
     1261 tatttttgct tgtggttcga ttacagcgac gagtcaagtg gcttatgaac aagcgaagac
     1321 catttacgat caattgagtg cgaaacaaaa aaagatgacc aaagtattga taaatgcaga
     1381 aggtaagtta tcccacccac taagtattca tgttcgtaat gaatggcaat tggcagatag
     1441 tcaattatta tttcaagaaa aataacattt tattttctta tgttacttat aagtagtttt
     1501 acatgctttt tgactttaaa aatacatgtg atgatacatg ttatatgaat tatcaatgta
     1561 cccagtaata tgctattttc attatgtaaa aaataacagg gggtgtgtag atggagattg
     1621 atgaaacttg tttgagtaac tcttataaaa gtatgaatga cttttttgag gaaatggaac
     1681 tggattcaga attgctctat caattttatc ttgcttttcg agggcaacag atttcttttc
     1741 caatgaaaat gtacgatcgt gagttagtta gaaaaagaat cgaaaacatg atagaacagg
     1801 aaaaaactgt agatatccgt caattgacag aagtttatgg tttctctgca agatggattc
     1861 aaaaagtaat taaacaaaaa gaaaggagac gagtacatgg ctaagctgat tgtattaggt
     1921 ggtttaattt acggtggata ttggttatat aaatatatgc ttgcgcatcc gaatatagat
     1981 aaaggaaaag taattaagac agttggggta atcatagttg gactcatagc tctaactaag
     2041 aaaaataata ctaatagccc aaaaataatg agaacttatc gctgtagagg atgtggaagg
     2101 gaaatttcta ctagttttaa tccatctgaa agccctcaag gaagagatcg ttgtcatgct
     2161 ggctatcgac attcttggga aaggattaac taaatgaaca ccattgagat taattgactg
     2221 ataaaataag tacgcgaggg agaaattgaa agggggaaat aaaatgaaaa aatggttgtt
     2281 attactaatt ggaatgggga ttttattcac tggttgtaca tctgagaata cgaggactaa
     2341 agagactgta gaaactgata caacaattga ggctaaaaaa agtaaaacgc aatcaactga
     2401 acaaactaca acagaaatga aacaatctgt ggaagcaagt acaagtgaaa gttcaatcat
     2461 tgaatcttct tctgaagagg ttaaaaatac tttgtgggat agtgagaaat ctactcaact
     2521 agcaattttt gtcccacaat ggggaaagac attaggtcaa gaatataaaa gctacaatcc
     2581 tcagaacaat gtaagtctgt atggtacgcc tttaccaagt gcagttattg atggaaattg
     2641 gaaaatggca atcaatgaag caccagttac cgtacaatgg tcagaagatg gaactggtca
     2701 tgcagatttc aatttagtag cagtttattc tgatgtagaa actggagaat atttagggca
     2761 gcatgtatat ttctttggct ttcaaaatgg acaacctaaa gtttatttaa cgcaacaaaa
     2821 tcaaggcaat gataaaaact acatgtactt caatgaaact cagaaccaac agttaaaaca
     2881 aggtttcatt gatattgtga atggacaaac tcctcaaact ccagtagtag aagaatctac
     2941 tcaacaagca acaactacaa cgacacaatc tacaagtagc tcagcaattt caagttggga
     3001 agctgctaaa gaaactgtac taaataataa agctttatgg ttgtatgatt ctcctgataa
     3061 aaatgcatca aatactaatt taattagcac aaatgaatat atgaaagatc taatgcaaga
     3121 tgaaaatgga agctactatt caattaatat tgtaacggat gataatggaa atacaacaat
     3181 ggctggtgga aaacaattcc gtgtttatac agatggaaga atttctcaac gtattggaat
     3241 gactaacaat tttcaaatga ttaaataatt gaaaatatta tttatcaaag aaaaactgat
     3301 tttgagaaaa attatttaat tttcttagaa tcagttttt
//
LOCUS       DACDXE010000147         3320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.152, whole genome shotgun sequence.
ACCESSION   DACDXE010000147 DACDXE010000000
VERSION     DACDXE010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.152"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..327)
                     /locus_tag="IWM23_002892"
     CDS             complement(<1..327)
                     /locus_tag="IWM23_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAP9885467.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPY"
     gene            964..1050
                     /locus_tag="IWM23_002893"
     CDS             964..1050
                     /locus_tag="IWM23_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAP9885468.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(1171..1860)
                     /locus_tag="IWM23_002894"
     CDS             complement(1171..1860)
                     /locus_tag="IWM23_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885469.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1867..2145)
                     /locus_tag="IWM23_002895"
     CDS             complement(1867..2145)
                     /locus_tag="IWM23_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885470.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(2105..3019)
                     /locus_tag="IWM23_002896"
     CDS             complement(2105..3019)
                     /locus_tag="IWM23_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9885471.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(3001..>3320)
                     /locus_tag="IWM23_002897"
     CDS             complement(3001..>3320)
                     /locus_tag="IWM23_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9885472.1"
                     /translation="PKQISFRVSESEFAKLKQSAEALQMSVPAFVKAKAQNARLVTPK
                     VAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSENLKIMQKELQKIWQ
                     QLK"
BASE COUNT          870 a          642 c          606 g         1202 t
ORIGIN      
        1 gtaaggattt tctacgtccc cgttgatttt aaattctgta aacattacaa acatatcgta
       61 agaaagccca gactcacttc tacgaccaaa gcgtaaagat aagatttttt cataagttgc
      121 ttttaaatct tctacaaatc gtacatttgc agttgctttg tagttactta aatcttttag
      181 ttggtcaaac gtaaaacgaa ctgtcttatc ccctttttca cgcattctag acacgatgga
      241 aaaaaacaaa ttcatttcaa ccggtgtaaa ttttcttaaa ggaattgtat ttaattcatg
      301 gtggtatttt acaatctcat tagccatata atcacctcaa tccaattatg aatgacattt
      361 tttaaatgtc aagacattca tatttgacgt tcaaacagac aaaatgtcct tcataaacag
      421 acaaaatgtc cttcataaac agacaaaatg tccttcataa acagacaaaa tgtccttcat
      481 aacattctgt atccattggt agagtaagga cacagcttac ggtaaagagg tttaaaaaga
      541 ggtttaaaaa gagatataaa aagagggggg ctaattttcc cgcctaaaaa ccacttcttt
      601 tttctttaaa caggttcgcg catttttcaa aactgccaat ccaaaacctt gcttcctacg
      661 gtcgccaaaa aggtgtgatc gctacgctct cacattgtac tgcgcagtcg cctaaaggct
      721 cctttgctaa aaacatgttg gtaaagaggc ctttgagcgg ttctgaatgc ttatctaagc
      781 cgttttaaga cagtcttgga taaaaatcct tatctagctt taaagcgcct tacaggcggt
      841 cagggcaagc cctaacaacc ctaaaataag cgatacttaa aaacaccaca caagtttaat
      901 tttttaagat ataataactt gtcgcctatc cgacagtctt gcgactggaa ggaggtgtat
      961 ctaatgtttg aattgttcat ttgccccctt cttatagaag tttgtgtctc tttattcagt
     1021 tattggttga ataaccgaaa caagaagtaa aatcaataaa ggcgacatca acctgaaaaa
     1081 aagccccaga gaatccgacc tctctggggc tttcgtgtat ctaatgtttg atcatttgca
     1141 tgtttagttt aacatgattg ccgatcaaat tcaattttgc tgttgctgat ctaaccattc
     1201 cttgacactt tttagataat ttaatgccgt ttcttttttc gttggatttt gtatgttcgt
     1261 tgcttgctga agcagtgctt tataacgttc gtaggtaact gattgttgcc gttcttttcg
     1321 ttcctgttca ctagttgaaa aagggattgt atcactgaat aaattataac aagcttcttc
     1381 tgtctgcgta tctttaaaat attgcacgtc ttttttggtt cgctcatagt tgtcttgaac
     1441 ctctttttgc tgtttttctg gcaagttttg ataaagacga gtgagttttt ccatgttttt
     1501 atccgtcaaa cgtttttctt gattcacaaa aatatcgttc atttcctcat aacaagtggt
     1561 aaactcttcc attttttgtg ttaacacttg ctcctttttc tttttatcac atgctgcttc
     1621 atacttttga gtgatatccc ctaataaaat acttcttgaa atacgtttat ttccctctaa
     1681 aacgccgaat aaaccactta aaacgattga taatgtaaat ataacccctg gcaataaaaa
     1741 cctcttaaat cgaattttag agccttttaa tgaatatctt gtttgataag ccttatagct
     1801 aagccaaata attcctaaaa tactggcaaa aatgagtaga tagggtaaat agaatgccat
     1861 ttctatttat tctccccata ccagccaaaa ggctaacagt gtcatgatta agcaacataa
     1921 gggggcaaga atatccattg atcgctgcat aattactttt ttctgttctt tatttcgctg
     1981 gtttaatagc gcatggtgga attttttgac ttgtgaatca attgtagttt gtattctttc
     2041 tttggtataa ggtgcaagaa aagcacgttg tttctttatt tcattttcta tgtcttgtaa
     2101 cgttttagaa cgataattct tgttggtagt ctgctcctct ttcaacgtgt tggtcatggt
     2161 ttcttcgagt cggtctaatt tccggttgtt cagtcgtaag agttcgttgt tctcttcgta
     2221 cttcttttgc agtttcgcga atgctagctc gagctcttcg tagctcgttg gttcgttgtt
     2281 gattgtaggc tcgttctcgt ctgtccagct catgtaaaat cgtctccttt tcgtaatcct
     2341 ttcctagttt gttggatcgt acttttttct ttttgtggat caattcgtaa gtaaaatttt
     2401 tcccacgatt caagcaatga atgccgaatg ttttaagaga atcacaaaat tggttatacg
     2461 ttcgagtttt tttgtttttc attgtttgat caatcgcaaa ccgaatttcg tctttccaaa
     2521 caggttcatt tttttctttt aatttgattt cagacatggt tcgtttttcg ggtttcttcc
     2581 ctcgttcagt gattgttaag tcatgttcaa gacaaatctc gtcatttagg tcataacatt
     2641 tgtttaaaaa tgcttgacca tgggcttgaa atttgttccc agtttccaga ttgacggagt
     2701 taatgacaat gtggttatga atatggtctt tgtctgtatg tgtgtaaatc gcgatttggt
     2761 ggtcaggggc tatttttttc gcatactctg cccctagttg attggctttt tcagcggtaa
     2821 tttctcctgg tcgaaaagac tggatcaata agtgtgcttg tacacgatcg tcttttccat
     2881 acagcatgcg aatttgtttc atttcgcttt tggcataatc aatgtcacag tttatccctg
     2941 ttttgacggt tgctcgtggt tcggcgtaat tgatcgctcg gctacaagaa gtggttcggc
     3001 ttactttaac tgttgccata tcttttgtag ctccttttgc atgattttta ggttttcgga
     3061 taagcgttgg gcgtcgccag gatcaacgtc gtgttggtgt gtgttgcacc acttggcaat
     3121 ttgattgata ttcccgccag ctttggctaa atcgcgcgcg attgcctgag caatgtctgg
     3181 cgccactttc ggtgtcacca gacgcgcgtt ttgtgccttg gctttcacga aagccggcac
     3241 actcatttgc aaagcttcag ctgactgctt cagctttgca aattcggatt cgctcactcg
     3301 aaaactgatt tgttttggct
//
LOCUS       DACDXE010000148         3274 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.153, whole genome shotgun sequence.
ACCESSION   DACDXE010000148 DACDXE010000000
VERSION     DACDXE010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.153"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            177..473
                     /locus_tag="IWM23_002898"
     CDS             177..473
                     /locus_tag="IWM23_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885473.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            480..2432
                     /locus_tag="IWM23_002899"
     CDS             480..2432
                     /locus_tag="IWM23_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9885474.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IWM23_002900"
     CDS             complement(2504..3001)
                     /gene="dfrG"
                     /locus_tag="IWM23_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9885475.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
BASE COUNT         1156 a          538 c          566 g         1014 t
ORIGIN      
        1 aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg
       61 caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta atttacacca
      121 caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt gtaggaatgc
      181 aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat tttcctatgc
      241 aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata tcagagcaag
      301 taagggtgat agttaagcaa tacgctgaat atgcagagcg tactgaaagc gaagcagtgg
      361 atgaattctt attgaacatt ttagacgata agaagtttat tgaatggatt gccaacaagc
      421 gaagtaataa gcggattgtt gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa
      481 tgaccaagct taaaagatta gccacgaagg aagatgaaat tgtagatgtt aaaattccga
      541 tttcaaacga agaattaaaa gatagggcta aacaatatga tttattaact ccaaaacgat
      601 ttgcaacaag gtataacaaa atgttgttct tgccaacgtc ttttaaatgg aacggttctg
      661 aatatcccat tcaatataat tactgcatca atccgttttg ttgtaatttt gggaaagaac
      721 agcacaaatt caaagatgta aaagggaaac caagtcgata taaaatgact ggtagttcca
      781 aagataaagg tcataaaggt atgtactgca atgacaatcc tattggcaga ggtgtatcgc
      841 aaaactgtac agtaacccct ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta
      901 tagaaatcaa tagcatccaa gatgttgagc cagattatca atttcataaa gaaggctgct
      961 cggaagaaga atcaacccca ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta
     1021 ggggcaaatc tcaacgctat caatgtaagg cgtgtaagaa gttcactaat gttctaccaa
     1081 aaagagaaga aaccactact tatcaccaac aaaagaacac cattcttcca atgttcgcta
     1141 agatggttgt tggaagggtt tcagtcagtc gtacttgtga tattttagga attggagttg
     1201 gaacctacta tcataagctg gagtggctgt ataggcgttg tttggagttt ttagaacgat
     1261 atgagacaca accccttcaa acaaagaaat ttaacgagat gtggcttaat acggacaaga
     1321 tgcactacta cctaaataac gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt
     1381 ttgaagacct taatatgcaa acgtatatag ttgtatctgc tgaggtctta tcaagatatg
     1441 tgttccgttc tgatgttgcc tatgattgga atatttcaat ggatgaatta aatgaagata
     1501 caagaaaatt caaagaagac catctcaata cttttagtcg taaaaatgat aggttagatt
     1561 ggtcttatta tccacaagaa ccatccgcta acgatagcga aaataggaat gcttatcttc
     1621 acgaattagg gaaaattact aataggagta gatttgtcga tggtttaaat gttgatgctc
     1681 cttatactac aactgctcac tattggttaa ttaaacaaat ggttaatgcc gacgaatggc
     1741 gaatgattag tgatgatgac ttctctatta gaaacgcctt ttatagagta ttcacgaaag
     1801 aattaaggtt gagcgatgca catcatttta tttgccaagt taacaagacg aaaagcagaa
     1861 aacaatgctt aaaagaattc ggacaagcaa aagcagaact gctcgattgg ggagatatca
     1921 gaggattcaa aacaaagttt ctacggactt tggcatctca ttatctaact gaattactaa
     1981 caagtcatca attccacgaa gaggctatta gtaaagatgg agaaagatat cgcaaatatg
     2041 ccgataaccc tatcaaacat ccattagcca ctaaggataa aggattttat tcagttgatt
     2101 gtagaacgga tttatctgct ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta
     2161 atgaccattc gaccaatagc tttattcaac agataagacg ttatatatca tctcttgaaa
     2221 gaccattaac aacagctcgt ggtgataaga agagctatat ttacgctaac ttcaatccga
     2281 agtatgctca atttgcgatt acaattctaa gaacttacta caacttttgt cgccctttta
     2341 aatcagcgga taaaaaggtt ttgacacctg ctcaaagatt agggattaca gataaacaat
     2401 ttgattggaa agatataatt tatttcaaat gacaaaaaga gcttggagta atcccaagct
     2461 cttgtgcttt atctttactg ttcttcttca attaacgcac ccgttaactc aataagtttt
     2521 ttctttcata tacatgaaaa tagtagttat acggattttt atcattcttt atcccttttt
     2581 gggcaaatac ctcattccat tcctcataat tcacttctgg aaaaaaagta tctccttcga
     2641 attcatgatg tatttttgtg atgtacattt tctcaacata agggaaaaac aaattataaa
     2701 tctgttctcc tccgaaaata aaaatttctt cttcgttttt acataactca aaaacatctt
     2761 ctattgaatg aacaatttca caaccattaa aggtaaaccc cttatctctc gtcagaataa
     2821 tatttcttct gtcaggtaag gctcttccga ttgattcaag gttcttccta cctaatatta
     2881 tcggatgtcc ctttgtagta tttttaacat attcccagtc cttgggaatc ctccaaggaa
     2941 tgtcattctc tttgccaatc actctattct tatccatcgc agcaatcaaa gaaactttca
     3001 tttataacac agccttttta actaatttac catctaattt cttatttcta tcaaaaaacc
     3061 ttttctcatt atcctgcccg ttagttgaat aagcaatttt ttctataagt tatattatca
     3121 taataccaca aaatcacaaa ttttcattca attatcattt tcaattgcat tttataaatc
     3181 attttgtatg taatttggta tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca
     3241 ttttgtcatt ttgtactcca aaactgaact actt
//
LOCUS       DACDXE010000149         3215 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.155, whole genome shotgun sequence.
ACCESSION   DACDXE010000149 DACDXE010000000
VERSION     DACDXE010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.155"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(22..2493)
                     /locus_tag="IWM23_002901"
     CDS             complement(22..2493)
                     /locus_tag="IWM23_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAP9885476.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            2766..>3215
                     /locus_tag="IWM23_002902"
     CDS             2766..>3215
                     /locus_tag="IWM23_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885477.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQ"
BASE COUNT          955 a          651 c          574 g         1035 t
ORIGIN      
        1 tcttttttga gatacttttc gtcaattttc tcgtttaaat atcgatagaa attttttagc
       61 tacgcgacta aacggctttt gctctttttc atttttacgt tcaaacaaat attctaccaa
      121 tgtggtcaaa tgcctaaaaa tcgaatctgc cgagaaaaat agaatgctca acagcaaaag
      181 tgatacagct gagataccgg tgattgaaaa cagcttatgc aaaaagatga ccgaaaaaaa
      241 gttaccgcta gcacagaaaa gaatgatctg acttctgaat ttattcagcg gtgaacagat
      301 tttatagaga acgaggaacc ctactgcaat caaaagcatg gtagatgctg tagaaacatc
      361 tgttttattt agattcaaag tcaccccgca gatcaccaac gcaccgacta cgatcatatc
      421 tgttagtcca cctggcacag ctttttccat cacattcatg atgaatttcc ctctgattcg
      481 ctttttgttt tcttctagag caagcaagaa agatggaagt ccgatagtaa acaaactaat
      541 caatgtgatt tgtgatggtt ctaaaggata ggtcagagca aagatcactg aaaacagtga
      601 gagcaaaaag gagaaaatat tcttcactaa aaacaagctg gctgatcgct caatattgtt
      661 cacaacgcgc cgtccttctg caacaacttc tggcatgcgt gaaaagtcag aatcaagtaa
      721 gaccacttgt gaggcctgca tggcggcttc atttccggaa gccatcgcta tactgcagtc
      781 tgcttctttc atggctagaa tatcattcac gccatcacct gtcatagcta ctgtattgtt
      841 acgctttatc aattgaacga agattttctt ttgttctggc ttgacgcgtc caaaaactgt
      901 atatttttcg acagctgctt catattcttc ttctgataat ttagatgcat caacgtactg
      961 atcagcatgt gaaatccctg cttggacagc tacattggat acggtgagcg gattatctcc
     1021 agaaatcact ttgatggcta cttgctgttt tttgaagtaa tcaaatgtgg ctcttgcttc
     1081 ttttctgata ggattagcaa tcagaatata gcctaatggt gtgactgctt ctgtcaatac
     1141 tggttcttca agaatcccat cgtacacacc aaagaccaac acgcgatagc ctttttcagc
     1201 gaaagaaaca aactcttccg catattccgg caattgatcg cgcaaaacca tttcgggcgc
     1261 tcctaaaaca tacaccttgt ctgccaacgc tacactgctg aatttttgga cagaagaaaa
     1321 aggaataaca tggtgatagg attgatcagt ggtttttgta aaatatgctt taacagcttc
     1381 cattgttgca ttgtctcctg ccatcgcttt tgcatagtca gcaatgcatg cttccaattg
     1441 atctgactga ctgattattt cttgtttttt agagacgatc attttttgaa cagacatttt
     1501 attttctgtg atcgttcctg ttttatctac acaaaggaca tttacccttg ccaatgtctc
     1561 aatgcttttc atattgtgta aaagaactct ttgctttgct agtttagtcg cacttaatgc
     1621 taatgcaatc gtcgtcagca agtacagtcc ttcaggtatc ataccgatca atgctgcttc
     1681 cattgcaacg acactttctt tgatcgtgtt tccattaaaa aagtaacttt gagaaaaaag
     1741 caggataccg ataggaataa tgatgatccc aatccatttg atcaattgat taagagaagc
     1801 aatcatctcc gactgttctc cttctcccat tgtcttggct ttgactgtca gctgggaaat
     1861 atacgaatct gctccaactt tgtctaatcg ggcgtaagct tttccggaaa caatgaaact
     1921 gccggacatt aatggatcac ctatttcttt ggttatttca tctgcttcgc ctgtcaataa
     1981 agcctcatta acttgcagtt tcccttccac gacctctgca tctgctggaa tttgatatcc
     2041 agtctctaaa ataacaatgt catctattac aaggtcttct atatctattg tttgttctat
     2101 cccgtctcgt atgacattga cttttgctgt atttaaaaca tttagttcgt ccaagatctt
     2161 tttggatcga atttcctgaa aaatggcgat tcctgtattg gctatgataa ctggtaaaaa
     2221 agtcaagttc ttataggatt ctactaaaca aagcaaaatg gctaagataa gaaagatcaa
     2281 attgaaatac gtaaatatgt tttccaatac gatttgctga tttgttttga atgtgggatc
     2341 gactgaatta tttgctgcac ctttttctat tcgttccttg acttgatctg ccgttaatcc
     2401 ttctttatac gatggaaaaa gacggatgac tttatctgat gatctttgtg ttactttctt
     2461 ttttttactt cgatttatcc atttattctt catgtggcta cctcgattga tttcgttttc
     2521 tggatatttc caaacttctc ctatccagta tagaacagat atggaaagtg ttcctcacat
     2581 taagcttgca aacacctttc attcctgtca tatagcaaaa aaaaagggtt ctataatatt
     2641 ttgtgttggt ggaaaatcga aggcgatttt ctactaacac tttttcttcg tttcaactca
     2701 aaaagagact cacttttggt aaaatttagt cgaccaaaac gtcatcctac caaaggagtc
     2761 tctcaatgga taatcatact agaaaattac tcggattaac agataaacat ttattttttg
     2821 atgaagaatg gctgattgaa aaagaatata aaggcgttca agcgcagttt attaaaggaa
     2881 aattggatga ctctccttcc tattgtgaac actgtcgatc ggttcgtatt atccgcaacg
     2941 gttcctatac gacacgcact cagatattga aagtcaaaga aaagttaact attttagaat
     3001 taaaacgtac ccgttttctc tgccatgatt gtggaaggac cttttctgcg aaaacggact
     3061 tagttgatga acatcatcaa ctaacaaagg aactgaagca ggcaattctc atggaactgt
     3121 acgaaaatca atcacgtaaa ctaatcgcta agaagtactt cgtctcggat gggacggtga
     3181 cacggatctt gcgtgaagca acgaagcatt accag
//
LOCUS       DACDXE010000150         3038 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.156, whole genome shotgun sequence.
ACCESSION   DACDXE010000150 DACDXE010000000
VERSION     DACDXE010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.156"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            193..807
                     /locus_tag="IWM23_002903"
     CDS             193..807
                     /locus_tag="IWM23_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885478.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(972..1796)
                     /locus_tag="IWM23_002904"
     CDS             complement(972..1796)
                     /locus_tag="IWM23_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885479.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(2434..2691)
                     /locus_tag="IWM23_002905"
     CDS             complement(2434..2691)
                     /locus_tag="IWM23_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9885480.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(2684..2953)
                     /locus_tag="IWM23_002906"
     CDS             complement(2684..2953)
                     /locus_tag="IWM23_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9885481.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1015 a          519 c          399 g         1105 t
ORIGIN      
        1 aatcttaaca gataaatcaa gttatatact agaaaattca taaaatatat ttttaatgtc
       61 ataatcgttt aaattttctt gattgacatt tatttacaaa aaatattgcc atttaaaaag
      121 aaaagtgaca tatggcttcc tcgtgtatga ttagggtgta ttttaaatag ttgcagaaag
      181 taggtaaaag ttttgagagt tgcatacata cgtgtctcat cgatcgatca aaatgaacaa
      241 cgccagattg aagaaatgaa aaaattcggt gcagagagaa ttttcatcga gaaacaatct
      301 ggcgcaacga ttactcatcg acctgttttc caagaagcat tagattacgt ccgagatcaa
      361 gatattttta ttgtggaagc tattgatcga cttggaagaa attatgacga gatcatcgct
      421 tcagtcaatt acttgaagaa gaaaaatgtg aagctgatca ttactagttt accgatcatg
      481 gctgaagcaa ttggcaatcc tctcctggat aaattcatta aggatctgat catccaaatt
      541 ttggccatga tcgctgaaca agaacggact gaatcgaaac gtcgccaggc acaaggaatt
      601 aagatcgcaa aagcaaacgg cgtctacaaa gggcggccca aattatacag tgcagacgca
      661 aaagatcccc aacgtcgcct tgtttacaaa agcatcgttg aagatcttaa aaatggtgtt
      721 gcgattgcta agattgctaa agactacaat gttacgagac aaaccgtgta tagaataaaa
      781 aaagacagca tggtcaatga caaatagttg atcatgctgc ttttattatc ttctcatctt
      841 taatttccta catataatta ctatctataa aattaagtct tactcagaac agataaagca
      901 taaaattcca acaaaccatt ttttacttaa ctaaataaag agtctgttaa tataatcttc
      961 acttatttac gttaatcccc tactgtattt gttggttcct tatctatatt ttctacaaaa
     1021 tccttattat cttgtgattt agcttcgtat aattctttgt atgtcgacac attggttata
     1081 ctaattccta tagctagttc ttgagactct acttttctct ctagctcctt tgacaaagtg
     1141 atttgagatt tatttggttc cgttggagtc aattctatag attcttcata aaaatctcta
     1201 tacataaagc tatcattaat tttataaaaa aaattaaatg tttttagcac tgccatataa
     1261 cattcaaaac attcttctat agttggttta cttccttgag gagaatgaac caatttatta
     1321 cgtttttcta tgaaattttt aatttccttt cttatttctt tcggaaactc aatattaaag
     1381 taccctttaa acatgctccc taataatttg tctacagggg gagattgtaa attctcaaat
     1441 attagagtta aatcaggttt ctcttgttga taaaattcct ttacccctat ttcacaggct
     1501 gtaattatcg aaataaacct atctctagta tttgatatat ttttacttct tttaaggagt
     1561 ataagagaaa aggggatact ttcgtgtcca tgcatgtaat taactaattg ttgagcttca
     1621 gaagttatat ctctaattat atagttaacg gaattaggaa gtgtctttaa ctctaatgaa
     1681 atttctatcc aattactatc ttctaatctg tatatgctcg aagtataatt tgattcgtca
     1741 gcaagcatcg ctacatccca aacactgatc ataaaatctt ttaaacgttc aatcatttca
     1801 gcaatattgc taaaatcagc taatttttct ttagaataaa aaaaacattt atatcagaat
     1861 caataaattc tatttttttt attactatat cgtcatcatt atcttgacca tttatgttta
     1921 ttgttccctg agtggggagt acttctttca aattcaagtt aaatttatat tccattactt
     1981 ctcctccaat atataaaaat tacttaactg tatccaaatt taatatgtaa ataaatcatt
     2041 aatttttagc taaattctat gagaagttta tttttgttgt ttttgctaat taactaaaac
     2101 tagtacaaaa aatttaaaat agattattaa acctattgct tattctattc agtcatatat
     2161 ataagcgtca tcctttagta actaacatcc cacactacaa tagtattata acatatctat
     2221 tcaacttcaa attgttgaaa tttaagccat ttatatttac ttttcctcaa ctttagaaat
     2281 gattttacta accttatatc cgtaatacgc gtaacaaaca cattcattaa taacaatcaa
     2341 cactaagcta tattagcttt ttaaatgact agggtaaata aaataaggat caaaaacttt
     2401 taagtttctg accctttcct tacttccgat tggttaatag tgatcttttg cagaataaat
     2461 aaatatcgtt tcattttcaa ctctatatat cagtctatgt tcatctgtaa ttcttctgga
     2521 ccattttcca gataaatcat gctttaatgg ctcaggtttt cctaatccag caaaggggga
     2581 acgatcgata tcttttatta acttgttaat cttttttata ttgcttttgt ttccttgctc
     2641 atgccaataa agataatcat cccaagcatc atcagaccaa gccttaatca tcagattcaa
     2701 cctcgattaa gtcatgtgtt ttaaatgcac ctttggagaa ttgttcatct cctcgacgaa
     2761 ttttttccat gacgtaatta ttagaaagtg ttctcaacgt ttcttgcata gaatcataat
     2821 ctctttttga taatacaaca actgtatctt ctacatcttt acttgttaca ataagtgttt
     2881 cagcatcctc attaacttgt ttcatataac tacgtaaatt ttggcggaaa tttgaataag
     2941 ctactgcttc cattcctttc accccttatt tatttctata aaacaattgt acattatatt
     3001 gtacaattaa gcaaatgatt aatattctaa cttcttat
//
LOCUS       DACDXE010000151         3019 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.157, whole genome shotgun sequence.
ACCESSION   DACDXE010000151 DACDXE010000000
VERSION     DACDXE010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.157"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1243
                     /locus_tag="IWM23_002907"
     CDS             <1..1243
                     /locus_tag="IWM23_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9885482.1"
                     /translation="KTKDQLNEFFANVLETPYWYAKKILSLLSECLDQPVYEEDIFFI
                     TLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANRMLNKNVFQAYDMPLDITLEKI
                     EAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHLNGPLLIIDHINTPLLLEVGQQ
                     VLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIITCCYTGIGSATQIQEILTKCLK
                     DAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGTEDPKINQIPYIGLDRLINGGS
                     IGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLTILDPDKVLSFVQKAIKELSKL
                     LNFDFSNNQLFLLYIHCSCMIERIVRKESVDEQEDIEEYMQANGEIMDKIHSAFFEIE
                     REYTIEIPDLELRLLSDIVQG"
     gene            1256..1888
                     /locus_tag="IWM23_002908"
     CDS             1256..1888
                     /locus_tag="IWM23_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAP9885483.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2093..2320
                     /locus_tag="IWM23_002909"
     CDS             2093..2320
                     /locus_tag="IWM23_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAP9885484.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2389..2655
                     /locus_tag="IWM23_002910"
     CDS             2389..2655
                     /locus_tag="IWM23_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAP9885485.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2645..2914
                     /locus_tag="IWM23_002911"
     CDS             2645..2914
                     /locus_tag="IWM23_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9885486.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1072 a          443 c          607 g          897 t
ORIGIN      
        1 gaaaaccaaa gatcagctta acgaattttt tgctaatgtt cttgaaaccc cttattggta
       61 tgcaaaaaaa atcttaagcc ttttatctga gtgtttagat caacctgtgt atgaagagga
      121 cattttcttt attacgctat atttttatag tttgaatgtc acgaatgtta aaacaggtat
      181 taagagtatt gtgctagctc atggttattc tacagctagt agtatggcga atgttgccaa
      241 ccgtatgctg aataagaacg tttttcaagc ttatgatatg cctttagata ttacattaga
      301 gaaaattgaa gcaaaattga ttcaattcct tggagattat cgaacagatt caggcttgat
      361 tttattggta gatatgggtt cactaaacca actaggcggt ttattagtga atcatctaaa
      421 tggaccactg ttaatcattg atcatatcaa tacaccgtta ttattggaag tcggccaaca
      481 agtattaaac aataaaagta tcaccgaaat aggagcagaa attgaaacaa gtcttacttt
      541 caaaaagcag ttactacttc ccaagaaaaa gaaaaagaaa gccatcatta cttgttgtta
      601 tacagggatt ggtagtgcga cccagattca ggagatttta acgaaatgct tgaaagatgc
      661 agttagagaa ttagtaatta ttccgtatga ttacaaaaag ctgttagaaa acaagaattt
      721 tgagactcct tttcaattat acgatgtcat catgattgta ggtacagaag atcccaaaat
      781 taatcagatt ccatatattg gattagatcg cctgattaat gggggaagca ttggcgaatt
      841 tgttcattac ttggaaaaag agctgactat tgaagtgaac gatttacaaa aagaattaat
      901 tttcaatttt tcaattagta aaattgtcga aaatttaacc attttggatc cagataaggt
      961 gctatctttt gtacaaaaag caattaaaga attatcgaag ttattaaatt ttgatttttc
     1021 taataatcag ctgtttttgt tgtacattca ctgtagttgt atgattgaac gaattgtacg
     1081 taaagaaagt gtggatgagc aagaggacat tgaagaatat atgcaagcaa atggagaaat
     1141 aatggataaa attcatagcg ctttttttga gattgaaagg gaatacacaa ttgaaatacc
     1201 agatttagag ttgcgattat tgagcgatat tgttcaagga taggagtgaa ttcgaatgac
     1261 aatgggagta atttttgata tggatggggt actcatagat agcgagacat tttattttga
     1321 gagaagaatg aacttctttc aagaactgga gatagatcca ggatcaaacc agattgatga
     1381 ttatgtgggg aaaacagaag ctgcaatatg ggaaacatta gttcctgagg acaaatcatt
     1441 aagagaggaa ctttatccga aatatgtaga ttatcgaaaa acgcatccaa ttgatttcgc
     1501 taaagcttta cgaaaagaag tgaaaccgtt acttgacttc ctcaagaagc gggatatacc
     1561 attggcactg gcatcttctt ctccagctgt cgagatagag agtatgctca ttcaatgtga
     1621 acttactgaa tatttttcct ttgttataag tggtgagtca ttaaaacaaa gcaaaccaca
     1681 tcccgaaata tatttaaaag cgaaacagca attagactgt gacttcttta ttgcagtaga
     1741 ggattcaacg attggtatta aatcagcaaa ggcagcaaat atctatacgg cggcactaaa
     1801 acaaccaatg caaatcgatc agagtgaagc agacatacaa attgaaagtc tacaagaact
     1861 ggaacaaatt gttttagaaa aaggttagtg aattaatgga aataaggcta tatatctgtg
     1921 ctagctattt tgactaacca aaaaaacttg ctataattca ggtgtagtaa aaggtgctgg
     1981 taacaccttt tacgaaaatg cttcggcagt gataatggga ttggcttttt aagtttttta
     2041 ttttgttcgc aatccctaag gtcatgtgaa tgatcctagg gattgctaga atattttgca
     2101 acagaaccaa aatacgaata gttatcaagc agccatattg actgcggtca atttaggagg
     2161 ggatactgat actactgctg ccgtaactgg tgctcttgcg ggtattcttt acgggggtga
     2221 agtgatccct gaaaattgga gaaaatcgct tcaaaatgac cagcttctta aaacgatcag
     2281 tcagagattt tgcgataccg tgctatctca aagaatttga ataatggata tgatgcaaat
     2341 cttgtttgta ctaacgttgt attgacaaaa aggattttat ttagtacaat gttagtacaa
     2401 atgatgaacg gaggtagaat catgtctcaa tcaaccatta catttaggat tcccgacgat
     2461 gaaaaagaac tggtattaga atatgcaaaa gcacacaatt cttcattgac cgaactttat
     2521 cgaaatgctg tactggataa aatcgaagat gaaatcgatc tacggacttt acggagtgca
     2581 attgaagttt ccaaagagaa aaaagagagt ggtatttctc aagaggaaat ggagaaactg
     2641 ctaaatgaat tataaagtca tctacatgcc gcaagcccag aagcaattaa aaaaaatgga
     2701 cagaaaccaa gctaaaataa ttgtcgcctg gatcagaaag aatttagaaa acacgactga
     2761 cccgcgtcag cacggaaaag gtttgactgg aaataagagt ggggaatggc gctatcgaat
     2821 tggaaactat cgaatattgg ctaatatttg tgattctgaa attgaaatcc agatattctc
     2881 aattggacat agaagtacaa tatataaaag ataaccgttt ttggaacgaa gtagagtttc
     2941 gaaagtcaat gtataatctt ttgaattttg cataatacca caggtagaaa ctacccgata
     3001 ctatcggttg gtttctgct
//
LOCUS       DACDXE010000152         2944 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.158, whole genome shotgun sequence.
ACCESSION   DACDXE010000152 DACDXE010000000
VERSION     DACDXE010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.158"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            6..977
                     /locus_tag="IWM23_002912"
     CDS             6..977
                     /locus_tag="IWM23_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9885487.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            970..1998
                     /gene="vanB"
                     /locus_tag="IWM23_002913"
     CDS             970..1998
                     /gene="vanB"
                     /locus_tag="IWM23_002913"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9885488.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2004..2612
                     /locus_tag="IWM23_002914"
     CDS             2004..2612
                     /locus_tag="IWM23_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9885489.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          780 a          624 c          812 g          728 t
ORIGIN      
        1 aaccaatgag aaaaagtgtg ggcattactg tttttggatg cgaacaggat gaggcaaatg
       61 ctttccgcgc tttatcgccg gattttcata ttatccctac gctgattagc gatgcgatat
      121 cggcagacaa cgcaaaattg gccgctggca atcaatgcgt tagcgtaggc cataagtccg
      181 aggtttccga ggcgacaatt cttgcgctga gaaaggtcgg ggtaaaatac atttctaccc
      241 gcagcatcgg ctgcgatcac attgatacga ctgccgccga gagaatggga atctcggttg
      301 gcacggttgc gtattcgccg gacagcgttg cggattatgc tttgatgctg atgctgatgg
      361 ccatacgggg tgcaaaaccc accatgcacg ccgtggcgca acaagatttc agattggatc
      421 gtatccgggg gaaagaactg ggggatatga ctgtgggagt tattggaacc ggccatatcg
      481 ggcaagcggt cgtcaaaagg ctgcggggat ttggatgcca tgtgctggcc tatgataaca
      541 gccgaaaaat ggatgcagat tatgtccagc ttgatgagct tctaaaaaac agcgatattg
      601 ttacgctcca tgtgccgctt tgtgcggata cccgccatct gatcggtcag aagcaaattg
      661 gagagatgaa gcaaggcgca tttttaatca acaccgcgcg cggagcgctt gtcgataccg
      721 gggcgctggt ggaggcgttg gaaagcggaa agctgggtgg tgcggcactg gatgtgttgg
      781 agggcgagga tcagtttgtt tataccgatt gctcgcagaa agtgcttgac catccctttt
      841 tgtcgcagct tttaaggatg ccgaatgtga tcatcacacc ccatacggcg tactacactg
      901 agcgtgtgct gcaggatact acagaaaaaa caatcaggaa ttgtctcaat tttgaaagga
      961 gtttacagca tgaatagaat aaaagtcgca atcatcttcg gcggttgctc ggaggaacat
     1021 gatgtgtcgg taaaatccgc aatagaaatt gctgcgaaca ttgatacgga aaaattcgat
     1081 ccgcactaca tcggaattac aaaaaacggt gtatggaagc tatgcaagaa gccatgtacg
     1141 gaatgggaag ccgacagtct ccccgccata ctctccccgg ataggaaaac gcatgggctg
     1201 cttgtcatga aagaaagcga atacgaaaca cggcgtattg atgtggcttt cccggttttg
     1261 catggcaaat gcggggagga tggtgcgata caggggctgt ttgtattgtc tggtatcccc
     1321 tatgtgggct gtgatattca aagctccgca gcttgcatgg acaaatcact ggcctacatt
     1381 cttacaaaaa atgcgggcat cgccgttccc gaatttcaaa ttattgataa aggtgacaag
     1441 ccggaggcgg gtgcgcttac ctaccctgtc tttgtgaagc cggcacggtc aggttcgtcc
     1501 tttggcgtaa ccaaagtaaa cggtacggaa gaacttaacg ctgcgataga agcggcagga
     1561 caatatgatg gaaaaatctt aattgagcaa gcgatttcgg gctgtgaggt cgggtgtgcg
     1621 gtcatgggga acgaggatga tttgattgtc ggcgaagtgg atcaaatccg gctgagccac
     1681 ggtatcttcc gcatccatca ggaaaacgag ccggaaaaag gctcagaaaa tgcgatgatt
     1741 acagttcacg cagacattcc ggtcgaggaa cgaaatcggg tgcaggaaac ggcaaagaaa
     1801 gtatatcggg tgcttggatg cagagggctt gcccgtgttg atcttttttt gcaggaggat
     1861 ggcggcatcg ttctaaatga ggtcaatacc atgcccggtt ttacatcgta cagccgctac
     1921 ccacgtatgg tggccgccgc aggaatcacg cttcctgcac tgattgacag cctgattaca
     1981 ttggcgttaa agaggtgata agtatggaaa atggtttttt gtttttagat gaaatgttgc
     2041 atggcgtccg ttgggatgcc aagtacgcca catgggataa cttcacagga aaaccggtgg
     2101 atgggtatga ggtgaatcgc atcatcggca caaaggccgt ggcgtttgct ctgcgcgagg
     2161 cacaaatcca tgcggctgcg cttggctatg gcttgctttt atgggatgga tatcggccaa
     2221 gaactgcggt ggactgcttc ctgcgttggg cagcgcaacc ggaggacaat ctcacaaaag
     2281 aaaaatttta ccccaatata gagcgagccg agttgattac aaagggttat gtggcctcac
     2341 aatccagcca tagccgtgga agcgcaattg atcttacgct ctaccacctg gatacagggg
     2401 aacttgtttc aatgggaagt aacttcgatt ttatggacga acggtcgcat catacagcaa
     2461 aagggatagg ggatgcagag gcacaaaatc gaagatgctt gcgtaaaatc atggaaagca
     2521 gcggatttca gtcttatcgc tttgaatggt ggcactataa gttgattgat gagccatacc
     2581 ccgataccta ttttaatttt gccgtttcat aatgaaagta ttggttttgt atcagatttt
     2641 ggatttgata aaatctatgc tggtcatgga caatctttta ctggaaagtg gttaaagcat
     2701 cttttgtaat acattgcaaa ttatagtatt caatctgccc agcggcagaa aagaaaaaaa
     2761 ccgctgttgg gcagaccttt cccacgccca ttttgtattt tgctggcggc ggttttgaga
     2821 aatcaaggcc gccgttcttt tctggcagca aaaggtgtgg cggcccatag gaggatgccg
     2881 ctatgctgtt ttgctattgc catgctctaa tccacttcca ccactcaaaa aagccttgcc
     2941 cctc
//
LOCUS       DACDXE010000153         2826 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.159, whole genome shotgun sequence.
ACCESSION   DACDXE010000153 DACDXE010000000
VERSION     DACDXE010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.159"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            798..1064
                     /locus_tag="IWM23_002915"
     CDS             798..1064
                     /locus_tag="IWM23_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885490.1"
                     /translation="MAKITKNEQENLVENYTKKPAYSNSRLDAHFVCTSNPKLSFDAM
                     TIVGNLNKNSAKKLSDFMSLDPQIRLWDILQTKFKAKALQDPLC"
     gene            1122..1634
                     /locus_tag="IWM23_002916"
     CDS             1122..1634
                     /locus_tag="IWM23_002916"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885491.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1631..2668
                     /locus_tag="IWM23_002917"
     CDS             1631..2668
                     /locus_tag="IWM23_002917"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9885492.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1022 a          496 c          564 g          744 t
ORIGIN      
        1 ttgatgacca cgaaagtgca tggcgatatt tgatgttgat actttatgca gtaccctatg
       61 tattcttcgc tatcattctg tggctcatag cattgttttt tagagtattt aatgaccttt
      121 aggtagattt atatataaaa agggctaaat acgcaaaata cccccttaaa atgcaaaata
      181 aatgcaaata agggggtaac tccttatgaa aaaacaacta atagtattat aacacgtttt
      241 tatgccgaga aaacttattg gttggaatgg gctatgtgtt agctaacttg ttagcgagtt
      301 ggttggactt gaattgggat taatcccaag aaagtaccaa ctcaacaaca cataaagccc
      361 tgtaggttcc gaccaataag gaaattggaa taaagcaacg aaaggagttg agtaaaatga
      421 gtgagaaaac gaagaaaatc attcgagtat cattagcttt ttcacaagta gtagtgcaat
      481 cattgatagt ttattatact taccagcaat ataaattatc taaagagaat taagttttga
      541 ttatgtaata agttttggta ctaaaaaaga cttgatctaa ttagaccaag tcttttgtta
      601 gtgttatatt aataacaaaa taaaaataag aagtcgctca ctccccgacc aaagtttgtg
      661 agcaaaataa acagtcgatg tttgtttctt aattgtatat cttgatatta aacgatttaa
      721 gattattctt caagatatat attcgcaaat gtaattggaa aagcgacttc ttaaacaaaa
      781 ttaaggagtc gctttttatg gcaaaaataa cgaaaaatga gcaagaaaat ttggtggaaa
      841 attacacaaa aaaaccggcg tactctaata gccggttaga cgcacatttc gtgtgcactt
      901 ctaatccgaa attgagcttt gacgctatga cgatagtcgg aaaccttaat aaaaacagcg
      961 ctaaaaagct atccgatttt atgagtttag atccacaaat tagactttgg gatatacttc
     1021 aaacaaaatt taaagctaaa gcactacaag accccctatg ttaggctagt tgtcgtatag
     1081 gataataagt tataataaag actatacgga ggatgattac catgacaaat aaaaacaatg
     1141 aacgttactc taccgaactg aaagcgacag ccatcaagaa aatgatgcca cccgaaaata
     1201 gggggattcg ccaattaagt caaactttgg gcattccaga agcgactctt tattcttggc
     1261 gtaaaaaagc tagaattcaa gggaatacta cacccggaac gaaaaaaaat gtcgaaaaat
     1321 ggtcaagtga agataaattt ttaattgtga tggaatcatt ctcaatgaac caagtagaac
     1381 ttgcggaata ttgccgaaag aagggcttat ttaaagaaca aattgaggct tggaagaaaa
     1441 cgtgcttatc tgcgaatgaa caggaagaaa atcgaacgag agaacttgca actgaactaa
     1501 aagaagagaa aaaacaagcg cgtcaattag agaaagaatt acgtcataag gaaaaagcat
     1561 tagcggaagc cgctgctctc ttattactaa gaaaaaaagc acacgcgatt tggggggacc
     1621 aagaggaaga atgatttcat cttcagatcg caaacaagct gttgaactta ttcaagaagc
     1681 caatcgcaat ggggcccgtc tcacctatgc ctgtaatgag ctgaatatta gcgttcgaac
     1741 ctacgaacga tggacgcgtg aaggtacgat agcgcatgat caacgccctt tggcgaaacg
     1801 tcctgtaccc aaaaataaac tgacggatca agaacgcgaa aaaataatag aaaccgtttc
     1861 aaaaaaagaa tttatgaatt tgccacctag ccaaattgta ccaaaacttg cggattgctc
     1921 gatttatatt gcatcagagt ctagcttcta tcgtgtattg cgagaaaaaa atatgcaaca
     1981 tcaccgtggc cgaagtcaag tttcacaaaa acgcataccg ccaagtcacc ttgcgacgaa
     2041 gccaaatgaa gtttggactt gggatattac ttggctaaaa ggtccaataa aaggactctt
     2101 ttaccggtta tatctcatca ttgatttatt tagccgtaaa atggtaggct gggagatttg
     2161 ggaatcagaa gaagctcgtc atgccgaaaa acttattaaa cagacggtgc ttaaggaacg
     2221 tatccaagga cagccgttgg ttttacactc cgataatggc agccctatga aagccgcgac
     2281 ttttcaagtc ttactcgaaa aattaggaat tcaaagttct tattcaagac cacgagtgag
     2341 taatgacaat ccttattcgg aagcggcttt ccgaacgtta aaatatcgac cagaatatcc
     2401 caataacggc tttaaaacaa tagatgatgc tcgaaaatgg acgaaagagt ttgtcgattg
     2461 gtataacgat tctcatttgc atagtgggat tcaatttgtg tctcctgcag cgtgtcatga
     2521 aggacgacat ctcgctattt tagagaaacg aaagcaggtt tacgaggacg ctaaaaagcg
     2581 ccatcctgaa cgctggtcaa aaggtgttcg aaattggact ccacatcaat cggtggcgtt
     2641 gaatccttta aaagaaacag aaagttaaca ggagcataat ctttcaagat tatcaaataa
     2701 ccctcgtcac ccaatagggg aaaaagttat tcttccccta ttggataacg aggccaaccg
     2761 accataggga ggttctgctt tttgataggt ggaaaataaa aatacgacaa ctatattgac
     2821 aaacac
//
LOCUS       DACDXE010000154         2749 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.161, whole genome shotgun sequence.
ACCESSION   DACDXE010000154 DACDXE010000000
VERSION     DACDXE010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.161"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1151
                     /locus_tag="IWM23_002918"
     CDS             <1..1151
                     /locus_tag="IWM23_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAP9885493.1"
                     /translation="EKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTNTPGNY
                     EGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDASEGIKNKED
                     LPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSDADKYNPEGQ
                     KVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVTYPDGSKEEV
                     EVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHGTADKNGNFT
                     VKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTDNQGSNSNQD
                     KNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1285..2628
                     /locus_tag="IWM23_002919"
     CDS             1285..2628
                     /locus_tag="IWM23_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAP9885494.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1098 a          356 c          516 g          779 t
ORIGIN      
        1 gcgaaaaagt agaaattgga ggcacagtag atttaacaga taatgttact aacttgccag
       61 cgttaccaga aggaacaaca gtaacagatg ttactcccgg cggtacaatt gataccaaca
      121 caccaggcaa ttatgaaggt gtcattgaag taacgtatcc agacggtacg aaagatacgg
      181 tgaaagttcc agtagaagtg acagacaatc gttctaatgc agataaatac aatcctgaag
      241 gacaaaaagt gactacagac ttaaataaag agccagatgc atctgaggga attaagaaca
      301 aagaagattt accaaaagga actacttatg cttggaaaga gaaagtagat gttagcacag
      361 ctggaaataa aaaaggtact attgtagtga catatccaga tggatcatct gatgaagttg
      421 aagtagacgt tacagtgaca gatactcgtt ccgatgcaga taaatataat cctgaaggac
      481 aaaaagtgac tacagactta aataaagagc cagatgcatc tgagggaatt aagaacaaag
      541 aagatctacc aaaaggaact acttatactt ggaaagaaaa agtagatgtt agtacagctg
      601 gaaacaaaaa aggtactgta gtagtaacat atccagatgg atctaaagaa gaagtagaag
      661 ttactatttc tgtagtagat aaaaaagcac cgaataaacc tcaagttgat cctattacag
      721 agggtgacca aattgttact ggtaaaactg aaccaaatgc agaggtgaca gttacattac
      781 cagacggaag tcaacaccat gggacagctg ataaaaatgg taattttaca gttaaagttc
      841 ctaaattaga ggcagggaca aaagtgatag taactgcaac tgatgaatct ggtaatacta
      901 gtgaacctac taatgtagtt gtttcttcca acaaaaaaga cagtggaaaa aacggtagta
      961 aaggtagtaa gactgacaat caaggtagca acagcaatca agacaaaaat agaggcaaaa
     1021 gccagtcctc aaaagttttt cctaagacgg gagagagtga cagtaatatc tttactatta
     1081 gcggtggttt aatactgtta ggaacgttag gattattagg gtataaaaat cgaaaaaaag
     1141 agaatgaata gaaaatcaat ttcttaagtc ttctctgaga agaggaagaa tctttttctt
     1201 cctctttttg cttttttatg atgggaaata agtgttagtt aaagatgaat atacttttta
     1261 tagaaaggaa aagaatgtaa ttaagtggaa aaaatagtaa ttattggttc aaaccattca
     1321 gggctagccg cagctcgatt tctaaactta agcgattcaa attatgaagt tattctaatc
     1381 gataaaaaat cagaaattag ttacttggaa tgtgggacat cgttatttat aaaaggagaa
     1441 gtaagtaatt tagaagatct tttttatcaa aatagaatga cactaaattc tgagataaaa
     1501 aagttatttc tagagacaga agttgacaaa attgatattc aaaataaaaa gattagttgt
     1561 gtaaaaaaag atggtacaag attctctcaa gaatatgaca aagttatatt agctacaggt
     1621 tcaaagcaaa ttatgctaga tataccgggg aataatttag ctaatatttt ttcgataaag
     1681 aattttccaa atgcaaaaga gatttttaaa gctttggatg atagtactat taaaaaagta
     1741 ggaattattg gagcaggata tattggagtt gaaattgctg aagccataag aaaaagaggg
     1801 aaagaagtat atcttttcga tgttgcagat agagtgttgt cgacttatta cgaccgtagt
     1861 ttttcagata aagttgaaga aatactatca aaaaatggaa tacatctttg tctatcagaa
     1921 agtcctaaaa aatatttagg aagaaaaaag attgaaaaaa tagtcactac aagtggtacc
     1981 caatactcta tggatatggt cgtacaagct ataggatttc tatctaatag tccaattggg
     2041 gaaaaggaac tcttgagaga tgttagtgga gcatatctaa ctaatgaata ccagcaaaca
     2101 aatataaaag atatatatgc tattggggat tgtgctacaa ctttttttac gtccattaat
     2161 agaagtagtg ttgatttttc tatttctaat gctctaagaa cagcttatat tgcaactcaa
     2221 catattaaca atcaagattt tacaccttct ggttcccaat ttacaaatgg cttttctata
     2281 tttaattaca atttttacgc agcaggtttg aatttaaaaa cagcacgatt attaagtgag
     2341 tctatagatt atatagaaat agaagatttc attagacctg cttttttgag aaaaaatagt
     2401 cgtgtaaaat taagaattag ctttgaaaaa aatacaaaga gaatagtagg tgtacaactt
     2461 tgtagtcaag aagatttatc tggactatta tcaatgtttt ctcttgctat agaagaacaa
     2521 attacattag acaaattgaa aatgttagat tatttatttc atcctagttt tagccaaccc
     2581 tataatttta ttttaaaggc agtaacagga ttaaacattg aaacataatc gttactaaat
     2641 gaaatcaaga aatatactgt attactcaat tcattgaaaa tatttatcat aatttttaat
     2701 agaaaacact attaaagaga aaaaagagag gtaaatcaaa tgaaaaaat
//
LOCUS       DACDXE010000155         2719 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.162, whole genome shotgun sequence.
ACCESSION   DACDXE010000155 DACDXE010000000
VERSION     DACDXE010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.162"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(45..1364)
                     /locus_tag="IWM23_002920"
     CDS             complement(45..1364)
                     /locus_tag="IWM23_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAP9885495.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IWM23_002921"
     CDS             complement(1361..2014)
                     /locus_tag="IWM23_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAP9885496.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..>2719
                     /locus_tag="IWM23_002922"
     CDS             2302..>2719
                     /locus_tag="IWM23_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885497.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVT"
BASE COUNT          904 a          432 c          280 g         1103 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgaca ccttctgttg
     2101 ccaatacttt ttcttttttc taataaatgc aaacagacac cttgaaatcc tttagaataa
     2161 agggttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tattgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaaactaa tcgctaagaa gtacttcgtc
     2701 tcggatggga cggtgacac
//
LOCUS       DACDXE010000156         2713 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.163, whole genome shotgun sequence.
ACCESSION   DACDXE010000156 DACDXE010000000
VERSION     DACDXE010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.163"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IWM23_002923"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IWM23_002923"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAP9885498.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1062 a          419 c          556 g          676 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ata
//
LOCUS       DACDXE010000157         2671 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.164, whole genome shotgun sequence.
ACCESSION   DACDXE010000157 DACDXE010000000
VERSION     DACDXE010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.164"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..321
                     /locus_tag="IWM23_002924"
     CDS             <1..321
                     /locus_tag="IWM23_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885499.1"
                     /translation="YKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEELVMLFLDPDFI
                     GKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLDGFGKPYPITH
                     LVKK"
     gene            735..1553
                     /locus_tag="IWM23_002925"
     CDS             735..1553
                     /locus_tag="IWM23_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAP9885500.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1626..1985)
                     /locus_tag="IWM23_002926"
     CDS             complement(1626..1985)
                     /locus_tag="IWM23_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAP9885501.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          925 a          372 c          471 g          903 t
ORIGIN      
        1 tataaagcaa tcattcctga gaacttagat gaggttaaac tttatttatg ggaagataac
       61 ggagaaattg tcggttttag tggtgtaagt gcagaagaat tagttatgct atttcttgat
      121 ccagatttta ttggtaagcg atatggtagt caaatattaa ttgagctgat tgaaactgaa
      181 gatataaaaa gaattgatgt taatagtcaa aataaatatg caaaacattt ttatttaagc
      241 catggatttg aaattgaatc tgaagaagac ttagatggtt ttggtaaacc ataccctata
      301 acgcatttag taaaaaaata attcatgaat ggtttattac ctgattgaac atgaataaaa
      361 gtaatatttt tattttgatg gcctatttct aggttatgaa ttatccagat caaatttgtg
      421 tattacattc agtaataagg tgcttatctt aggatcaact tatgatacta gctgaccaag
      481 gatgtaccag ttatagaaaa tcagttaaag taaaaatgat aaaaatctta tgtcaacact
      541 gatttaggct gacctgtggg ttaggagaaa gacaggtcgg tagcttttaa tacaaaaaat
      601 ggcttgtatg tatctagata gttaactgaa attagaaatg ttcttatttt taacggagta
      661 agaaaaatag gctgctttag gtctgaatac tttttcaata taatgaagat atcaaaggcg
      721 acaggagtgt ttttatgaat aagaatcctc ataatctaaa gttggtagca gtagatatgg
      781 atggaacatt tgctcgtact gattacacat acgatatccc aaggttcaag gctgttttat
      841 cgcgaatgaa agctgttgat tgtcaatttg ttgttgcaag tggaaatcaa tactaccaat
      901 tgagatcgtt atttccaaat tattatgatg aattaagttt tgttgctgaa aatggtgctt
      961 ttgttaaaga taaaacagag gtactatttt cagccgatat gcctagaaaa gcgatcgagg
     1021 tggtccttga tttatgtaaa gactatcctg aagtaaaaaa tgtgatgtgt ggtctgaata
     1081 gtgcctactg tgaaagagga aaggtgagtc aagcattttt tgatctaacc aatatctatt
     1141 accatagatt agaatgggta gctgattttg cagaagttca agataaaatt ttaaaatttg
     1201 ctccgactat gcctgttgaa aaaaccgaat tttacgttga attgttccgt gaaaaattag
     1261 ccgggctatt agtacctacc agtagtggtc atggttctgt agacttgatt ctttctgagt
     1321 gccataaaac tttcggcttg aaaatgttag ttgagcgctt ggggattaat cctgaccagt
     1381 gcgttgcttt tggagatggg ggcaatgaca ttgagatgtt ggagtattgt ggtctgagtt
     1441 atgaaatgga taatgcaacg gaagctgtta agcaagttgc taagcatcaa tgtccttcaa
     1501 atgacgaaga cggtgtttta gtgacgctcg atcgattgtt ccctaacacg taaacactag
     1561 gtaattcatc aaaaaacctc gatataagaa atattcgagg ttttaaaact atatttagaa
     1621 cagatttacg aagcgtctga tgagtcaaaa tagacttgga cattgctatc taaaatacca
     1681 ctttcattct ttaaacgaat tgtttgattt gatggagtta cctctaatac taaatgccca
     1741 ttaataaagg tagtatcgta ttcagatgtt ttaactgttg cttcttcgca aggtattgtt
     1801 aaatcatatg ctggaatata atgttgtgtg gaaactaata aaaaatgatc accaatcata
     1861 cagcatccct ctgctgaaga aaattgatta gagccatcat ttaaagttaa caagaatcca
     1921 tcctttaaac gaggtacttg cttaaatact tctggttgta cttctaaaaa taaattatcg
     1981 ctcattttca cgaccaccat cgacttccac agttgaaatt gacattttat ttaacacacc
     2041 tattccccct taactataac tcaattctat ataaaaaata ttctattctc aaataattga
     2101 acttttcaaa tcgccaattt acttcggctt tatcagagct cattcatatc aagagcgatt
     2161 aacgctgtaa aaattaaacc gaagatctga taaattttct ctattttcta caatatttaa
     2221 aacgcttcct tagtctatta tatatctatt tctcttagga gtatgtttag atgtttgaat
     2281 aatagttgcc caactttaaa ggttattctt ataatatttt acttcatttt ccaatagaaa
     2341 attcaaagga ttactggttt ttctaaaata gtttagttta aatacataag tatcctccga
     2401 cactgtatta gttcataatc atctaaatac aaaattttca aataaaaata tttcaagctc
     2461 tatcgttgac gaatgtaaac gatacttata aagtaataga aattgattac atttgtaaac
     2521 ttaaaatggt taatttatct cacataaaac taagaataaa ataatgatta aaagtggtta
     2581 gtttttatac gatttagcct atcggtaaaa atgaattaga gagtgtaaaa tattttgtgt
     2641 aaatgaaaaa atccatacaa aaaaggaagt c
//
LOCUS       DACDXE010000158         2634 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.165, whole genome shotgun sequence.
ACCESSION   DACDXE010000158 DACDXE010000000
VERSION     DACDXE010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.165"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            5..1150
                     /locus_tag="IWM23_002927"
     CDS             5..1150
                     /locus_tag="IWM23_002927"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAP9885502.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="IWM23_002928"
     CDS             1321..1779
                     /locus_tag="IWM23_002928"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAP9885503.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="IWM23_002929"
     CDS             1938..2408
                     /locus_tag="IWM23_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAP9885504.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWM23_002930"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="IWM23_002930"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          862 a          505 c          496 g          771 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgtcagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc accccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DACDXE010000159         2526 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.166, whole genome shotgun sequence.
ACCESSION   DACDXE010000159 DACDXE010000000
VERSION     DACDXE010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.166"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(361..582)
                     /locus_tag="IWM23_002931"
     CDS             complement(361..582)
                     /locus_tag="IWM23_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885505.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(680..925)
                     /locus_tag="IWM23_002932"
     CDS             complement(680..925)
                     /locus_tag="IWM23_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885506.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1165..1779
                     /locus_tag="IWM23_002933"
                     /pseudo
     CDS             1165..1779
                     /locus_tag="IWM23_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2149..>2526)
                     /locus_tag="IWM23_002934"
     CDS             complement(2149..>2526)
                     /locus_tag="IWM23_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9885507.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          845 a          440 c          346 g          895 t
ORIGIN      
        1 gacgtctttt ttgaattgtt tgcctttaaa atgattcatt actctgtcct ctctgtcttt
       61 tttctcaatt ttacactaaa atagattttt tggaaaactt tgcaacagaa ccgtttttac
      121 tatatacttc ttcccagaac ttgtccgtct ccttcaaatt atctctacct ctcgagttca
      181 ttgggtggtt tgcccattta ttaaacatgg aatcaccttt ctcatactca tatatttatg
      241 tcctatttca attatattta tatatgctta aataaatttg tatcctgaaa tttattgtaa
      301 tatgaagctg tattttacat atagtaacca aagagttaat aaaataaaaa gagcgaacca
      361 ttatagatca gctctttttt cattgctgtt tttaatctta tctagaatca cttcaaatgt
      421 ttcaaaggta tttacatcta actgacataa ccaaagcaag tacttctttt ctttgtaagt
      481 ccaatcaata ttacggtaag aggacattag attatggtac cttgctcttt gttcaactga
      541 agccaattcc cagcgttttt caaatatttt ttcttcttcc attattgatc cctcgcaaga
      601 cgctacctca aacttaaata atgggcagcg aaagtctaat tccttagatc taagtatagc
      661 aaacaaatat gaaaaaaact tagaaggtag taaaagttct tccgtctttt gctttgcttc
      721 tattcttaat gtgtttcgtt atcggtgccc tacaaagatt ttcattaatt acacactcat
      781 ttattgttgt gtattttctg acatttctct cttgatcaat tattttaatt ccatgggcat
      841 cctgatagtt cataggagag gagcggctat cagaaatagt tgcacctatc tcttgaactt
      901 cttttacaac ttctggaaaa tccatccatc ctgcttcagc tgtcaaaagt aataatctat
      961 aatatttatc tttccttttc aatctaagaa atcgtcaccc cattcttttt cttctattat
     1021 aaactattta acttttagaa ttcaaaaacc gtttgtctat ttagagttta ccctaaatag
     1081 acaaaaatat aacattgaaa aatttagagt tctaacgtat gattagggtg tattataaac
     1141 gttatagaaa agtagggaat aaatttgaaa attgcttata ttagagtttc atccatcgat
     1201 caaaatgaac aacgtcaaat tgaagaaatg aaaaagtttg gcgctgaaaa aatttttatc
     1261 gaaaaacaat ctggagcgac gattagtcat cggccgattt tccaggaagc tttagatttt
     1321 gtcagagagc aagatatttt tattgtagaa gccattgatc gacttggacg caattatgac
     1381 gagattatcg attcagtgaa ttatctaaag aagaagaatg tgcgacttat tatcaccagt
     1441 ttaccgatca tggctgaagc aattggaaat cctctcctag ataaattcat caaagactta
     1501 atcatttaaa tctcagccat gattgctgaa caagaacgta ctgaatcgaa acgacgccag
     1561 gcacaaggaa ttaaaatcgc taaagcaaac ggcgtctata aaggtcgtcc taagttatac
     1621 agtgcagaca ccaaagatcc tcaacgtcgc cttgtctata ggagtatcgt tcaggatctt
     1681 gagaatgggg ttgctatctc aaagattgca acagactaca atgtgactag gcaaacaatt
     1741 taccgaataa aaaaagaaat cgaccaacta attgtatgat ttcttttttg aatcacgtaa
     1801 ttattaatta gctttgtcat caatacagac aatcatttta gattcatcta gctaagttga
     1861 ttacatttat gttaaaaaga atttagttaa cataaatata attgatgcat aaatcgtcct
     1921 tatttaaagg catttaacat tgacaactta gtataatgta tcttatgtaa actaataaga
     1981 tttgaatttt attttataca cactcgataa tagatcaatt ttatggtccc ccgcatttac
     2041 gattgcacaa gagaaacata aagtcgacct attagcattt cttttcaatt caaacattaa
     2101 aaaagtttat acctctgcta ctaaaaaatg aaattatcta tttaaaattt aaaacggtaa
     2161 atctgattca tccaaattgg aaaatggatc agcctgtatt ggagctttgt atttctccat
     2221 aataacagat atcttttttt cattttttaa aaaatacctt ttaaatttct tatagtcctc
     2281 tgaaatcaaa atatagcgtg atggatactc atcaaaagaa gaatcgtatt cttcctttaa
     2341 aattcccgat ttacttaatt tttctagtac atcaaatact ctatcactca agtaactcct
     2401 taaattgtta tcactttccc attgaactac ttttctataa gttgaattat cagttgaaaa
     2461 gatagaattt tcatccataa acgcatatat tagcagcaat ctttcatcat tgtcaaattc
     2521 tattgg
//
LOCUS       DACDXE010000160         2500 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.167, whole genome shotgun sequence.
ACCESSION   DACDXE010000160 DACDXE010000000
VERSION     DACDXE010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.167"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1125
                     /locus_tag="IWM23_002935"
     CDS             <1..1125
                     /locus_tag="IWM23_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAP9885508.1"
                     /translation="TKDGRFKFIERYVDPYTEKTRKKSTTLTSNSPQAWKKAQKILDK
                     KIKEALENYNKSDITFDELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHISDDTIVRN
                     IDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPALAVKIHPKK
                     VEEEKRKLSMDKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRYGELLALQM
                     KDYEDGKISINGTLDYSSVKMDNAIKTTPKNTYSQREVQLPNRAKELIESVIADNILA
                     GRLTDPDQYIFISTSGTPLTLHSFNAVLHKVEEELELEKSLSSHIFRHSHVSLLSELG
                     VPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            1223..1456
                     /locus_tag="IWM23_002936"
                     /pseudo
     CDS             1223..1456
                     /locus_tag="IWM23_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
     gene            1648..2481
                     /locus_tag="IWM23_002937"
     CDS             1648..2481
                     /locus_tag="IWM23_002937"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAP9885509.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT          829 a          449 c          451 g          771 t
ORIGIN      
        1 accaaagatg ggcgattcaa gtttattgaa agatatgtag atccttatac agaaaaaaca
       61 cgaaaaaaat ctacaacact tacaagtaat tcaccacaag cgtggaaaaa agctcagaaa
      121 attctagata aaaaaattaa agaagcactc gaaaattaca ataaatcaga tatcactttt
      181 gatgagctgt ataaagaatg gtatgaatat tataagcagc atgttaaacg tactagctat
      241 ctgaaggtgc caatgatgat gaaacatgtt tctaagcaca taagcgatga tacaatcgtt
      301 agaaacatag atgagacact cattaataag ataattgaag atatgtatac gtttggtgac
      361 ctctcactga actacacaaa acaaacaaaa acaactctat ctgttatgct aaactatgca
      421 atcgatagaa aatacatcca aagaaaccct gctctagcag ttaaaatcca ccctaaaaaa
      481 gtggaagaag aaaaaagaaa gctttctatg gataaaaaat atctggagaa agaagaaatt
      541 gatcaaatac tgaaacagct atactccaat cctcgcagaa aactacacgg cataatagct
      601 gaatttttat atttgactgg tttgagatat ggggaattgc tagctttaca aatgaaggac
      661 tatgaggatg ggaaaatttc cattaacggt accttagatt actcatctgt gaaaatggat
      721 aatgctataa aaacaactcc aaaaaatact tattcgcaac gcgaagtaca attgcccaat
      781 cgtgcaaaag aattgattga aagtgtgata gctgacaaca ttcttgcggg tagactcaca
      841 gatcccgatc aatatatatt tatatctaca agtggcactc cgcttacgct gcactcattt
      901 aacgctgtac tccataaagt agaggaagaa ttggagttag aaaaaagtct atcatcgcat
      961 atatttagac atagccatgt ttcactatta tctgaattgg gcgtacctct taaagccatt
     1021 atggaacgtg taggacattc tgatgcaaat acaactctgt ctatctataa ccatgtaacc
     1081 aaaagagcca aacaacaagt aattgataaa ctaaatagcc tttgatattt ttgccccttc
     1141 tttgcccctt ttacttaaaa taaaacaaaa aaagaagaaa gaaccctgtt aaaatagggt
     1201 tctttcttaa ttactacatc atatcgccca tcactgcgaa aaaaatggtg gtggaaatgt
     1261 tttagtcgta tctcatggtt tgacgatcgc ctttttgctt aatctgatcg atcctaaaca
     1321 gcctgttcgt gctgggcttt taaatggcag cgtaacaaag gtcacttatg caaacggtcg
     1381 attttctatc cacggcatca atgatacgtc ttatatcgat aacgggaaaa cacatagtcg
     1441 cgactcgttt ctataatggg gatattttaa tttttaccag agaatcataa aaggcaaagt
     1501 ctgaacgagg gctttgtctt cttttttctc ttcgtgtact tatctgcttt atttctcaag
     1561 tttttcttaa tattaaaaaa cagattgggt ttctcaaaaa ataacggtat actataagat
     1621 gtatcttaat tgggaaggaa ggaatttatg ttttttgcaa atattatcaa agcagtgatt
     1681 ctcggtatta ttgaaggaat aacggaatgg ctgcccatca gtagtactgg tcatttgatc
     1741 ctagcagatg aattcatcaa actggatgcc agttcacaat ttatgtcgat gttcaatgtc
     1801 gttatccaat taggggcgat cttggctgtc gtcgttcttt attttcacaa gttaaaccca
     1861 ttttctccaa gtaaatcaac ggtagaaaaa aaagatacgt ggacactttg gtttaaagtg
     1921 atcgttgctt gcataccagc catcatcatt ggtttgcctt tggatgattg gttagaagca
     1981 aatttccaca agttcttacc agttgccatt gttttgatcg tttacggtat tgcctttatt
     2041 attgtcgaaa aacgcaacaa aaacaaaaca cctcgttgga cttctctgaa tgactttacg
     2101 taccaagcag ctcttcttgt tgggttattc caagtcttgg cacttgttcc cggaacatcc
     2161 cgttctggcg caacgatcct aggatctatc ttgattggtg cttcgcgttt tgtcgctaca
     2221 gagttttcat ttttcttagg gattcctgtc atgtttggcg caagcggctt gaagatcgtc
     2281 aaatatttag ctgaagggaa ttcattcaaa atggaagaaa ctgttgttct tcttgtagga
     2341 acattcgtat cttttgtggt ttccgtctta gctatcaaat tcttactaag ctatttgaag
     2401 aaaaacgact ttaccgcttt tggctggtat cgtattatcc ttggtatcat tttgattggt
     2461 tattggttta ttgcaatgta gaaaaaacca gaacacttct
//
LOCUS       DACDXE010000161         2473 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.168, whole genome shotgun sequence.
ACCESSION   DACDXE010000161 DACDXE010000000
VERSION     DACDXE010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.168"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            7..624
                     /locus_tag="IWM23_002938"
     CDS             7..624
                     /locus_tag="IWM23_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885510.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..>2473
                     /locus_tag="IWM23_002939"
     CDS             1644..>2473
                     /locus_tag="IWM23_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885511.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     "
BASE COUNT          905 a          326 c          397 g          845 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt tta
//
LOCUS       DACDXE010000162         2412 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.171, whole genome shotgun sequence.
ACCESSION   DACDXE010000162 DACDXE010000000
VERSION     DACDXE010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.171"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            123..515
                     /locus_tag="IWM23_002940"
     CDS             123..515
                     /locus_tag="IWM23_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885512.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
     gene            complement(688..870)
                     /locus_tag="IWM23_002941"
                     /pseudo
     CDS             complement(688..870)
                     /locus_tag="IWM23_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            887..1054
                     /locus_tag="IWM23_002942"
     CDS             887..1054
                     /locus_tag="IWM23_002942"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885513.1"
                     /translation="MENTFELYNKTTVDKLIPILVPTDLNGHDVLLRFNGFLGEDYRY
                     LKISDPQPLTE"
     gene            complement(1097..1672)
                     /locus_tag="IWM23_002943"
     CDS             complement(1097..1672)
                     /locus_tag="IWM23_002943"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002984668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAP9885514.1"
                     /translation="MAQLFYRYGTMNSGKSIELLKVAHNYEEQGKRVIIMTSAIDTRS
                     GFGKVESRIGVSKPAIAITSEMDVYGELVEIIDQYGKVDCILVDESQFLSRENIIDLS
                     LIVMNEEVPVICYGLKNDFQNNLFKGSEALLTFADKIEEIKTICHFCNKKAVMNARIV
                     DGIPVYSGEQIVVGGNEDYLSVCRNHYYNED"
     gene            complement(1827..2353)
                     /locus_tag="IWM23_002944"
                     /pseudo
     CDS             complement(1827..2353)
                     /locus_tag="IWM23_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          765 a          413 c          377 g          857 t
ORIGIN      
        1 attttttctt ttaagtaaaa aatataggtg attttttttg acttcatatt tataataaag
       61 acacgtaaat caattatttt tcattttact ttaactaata atgtttattt ggagggatta
      121 ttatgttaga tgatagattg acgtgttcta attgtggtac attattttac tatgaggaag
      181 ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat acgttgtcca aattgcaatg
      241 aagttattaa gcgaaagttg tctcatggtt attttgtcag ctacgaacta tatgaagaaa
      301 gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa tttgaaaact ggagctatta
      361 taactgaaaa agaatttaaa aatttagtga ctgaagaaga aagcaggttt tataatttgt
      421 ctggtaatat ttgccctgga tttatggatc agattattga aaatgagtca attgaatcaa
      481 ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa ataatcctgt ttttacagtg
      541 aattatttta attttgattt tcaataaaaa tatattacaa taataatcgt aatagctaat
      601 gaaacaattt ctcaatcttt ttaccactcc aaacagggag cgtcaataat tttgtgtaaa
      661 taactcgtcc ttctgcaaaa taatgggtta ctcagtaaac attgaagcta atgtatccgt
      721 cacttgttgg aagcctttat ggcttctatt taagaatttt tgattgtatg tatcaaagat
      781 ggatactagg aaacgctcta gtgattcttc attttgaaac tgctcttttc tgcggctgta
      841 tttcttgatt tgtttattga aagattcgat tgtagaacca gaaggaatgg agaacacttt
      901 tgaactttac aacaaaacta ctgttgataa attaattcca attttagttc caactgattt
      961 aaatggtcat gatgttttac ttcgttttaa cggattttta ggtgaggatt atagatacct
     1021 aaaaatttct gatcctcaac ctttaacaga ataataaaaa acgtgtaatc aattatttga
     1081 ttacacgttt tttatttcaa tcttcgttgt agtagtggtt gcgacatacc gaaaggtaat
     1141 cttcattacc tcctactaca atctgttcac ctgaataaac tggaatacca tcaacaatac
     1201 gagcattcat tacagccttt ttattgcaaa aatggcaaat agttttaatt tcctcaattt
     1261 tatcagcaaa tgtcaataat gcttcggatc ccttgaagag attattttga aaatcattct
     1321 tcaatccata gcaaattact ggtacttcct cattcattac gataagtgat agatcaataa
     1381 tattctctct acttaaaaat tgagactcat cgaccaaaat acaatcaact tttccgtact
     1441 gatcaataat ctccactaat tctccgtaca catccatttc agaagtaatt gcaatagctg
     1501 gtttcgaaac acctatacga ctctcaactt ttccaaatcc tgatcgagta tctattgcgc
     1561 ttgtcattat gataactctc ttcccttgtt cttcataatt atgagccact ttcagtagct
     1621 caatactctt tccactattc attgttccat atctgtaaaa taattgtgcc attaaattta
     1681 ttcccttctt ttacaattca attattcctt aattccatct gcttttatgc agctgctcgt
     1741 ttattccttg atatgtctat aacatctact atccctaaaa ccaatttata agccattaga
     1801 gatggtattc ctgaacatct ctcatattgt tttttgagtg gtatatcatc tgaaaaactg
     1861 aaggaatcat gaaaatagcg acatacttat catttagagt tctaacttta aatgcattta
     1921 cttcttcagt cagcacttta gtcatgttgg acatggtttg gggcgtgtag tgatgcccat
     1981 acattttctc aatcaagtct gcgatttccg acatggtaat accttttcgg aagaggtgaa
     2041 tgacggtctc ctctaatgtg tcattcgtcc gtctataagc aggaacagtc tgttgcttga
     2101 actcgccatt gcggtcgcgc ggaatctgga gatgaagttc cccatactcg gtcttgaccg
     2161 tacggtcata ggagccgtta cgagaattac ccgtgttaaa accaatgcga tcgtactttt
     2221 cgtaatcgag gaaagccgtt aactccgttt tgagaagcgt attgacagct ttttccaaat
     2281 ggacacggaa taatccattc aaatcgcctt tgttcgctag agtctttagg atttctgtag
     2341 taaaatcgtt catatggaag tcctcttttc tgtgaatgtg ttgtggtaac tctattctac
     2401 agaagggact tc
//
LOCUS       DACDXE010000163         2400 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.172, whole genome shotgun sequence.
ACCESSION   DACDXE010000163 DACDXE010000000
VERSION     DACDXE010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.172"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            207..2069
                     /locus_tag="IWM23_002945"
     CDS             207..2069
                     /locus_tag="IWM23_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9885515.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2400
                     /locus_tag="IWM23_002946"
     CDS             2069..>2400
                     /locus_tag="IWM23_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAP9885516.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          866 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
//
LOCUS       DACDXE010000164         2375 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.173, whole genome shotgun sequence.
ACCESSION   DACDXE010000164 DACDXE010000000
VERSION     DACDXE010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.173"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..832
                     /locus_tag="IWM23_002947"
     CDS             <1..832
                     /locus_tag="IWM23_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885517.1"
                     /translation="RQTIHKVYLFFKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSE
                     YIQRKVVQGWTPDVIVGRAAFPISCSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQER
                     RGKQTFRRSIHEREKDYSQFSNEFGHLEGDTIVGLKHKSAVITLVERLSKVIITLKPC
                     GRQAIDIEKKLNQWFESVPKNLFKSITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQ
                     RGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF
                     "
BASE COUNT          871 a          316 c          474 g          714 t
ORIGIN      
        1 aagacaaacg atccataaag tatatctgtt cttcaagcaa ggaaaatcag ccttagaata
       61 ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga cgtccgcttg ttttacccga
      121 ggaacaatca gaatatattc aaagaaaggt tgttcaagga tggacacccg atgtgattgt
      181 tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc atttatcgta tgttcaaaaa
      241 ggggctattt gattcttctg acttaccgat gaaaggcaag cgtaaaccga atggacatca
      301 agaaagacgt ggaaaacaaa ctttccgccg ctctattcat gaacgtgaaa aggattatag
      361 ccaattctca aatgagtttg gtcaccttga aggtgacact atcgtaggtc tgaaacataa
      421 aagtgctgta attaccttag ttgaacgatt atcaaaagtt atcatcacat tgaaaccgtg
      481 tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa tggtttgaat ctgtaccgaa
      541 aaacctattc aaatccatca cttttgattg tggaaaggaa ttttcaaatt ggaaacagat
      601 cagtaatgtc aatgatattg ccatttattt cgctgatcca ggaacgccgt ctcaaagagg
      661 cctaaacgag aattctaacg gattgttacg tagagatggt ttattgaaat ctatggattt
      721 caattcagta gatgaatttt ttattcaatc tgtcgcatct aaacgaaata atattcctag
      781 aaaatcactg gattatcgaa cgattatcga acacctttgg aagtattttt gagttacgta
      841 agtattgatg atctgtctaa tttaatttga caattaagaa agtattaact tgttttgtga
      901 aaaagagaat ttgaaaaacg attggcgaac aaatgctcgg taggtatggt ataaatacag
      961 agctaattgg tgtgatgaaa tcagatattc gcgtgagatt cagtaaagta ggagttcgaa
     1021 aacagctagg tgacaaacag tattgctttg gtacttcgga atggtttatc aaattcttat
     1081 caacagttca cttctcttta tgtttgagtg atgattcgtt aggttattgc ttggaaagac
     1141 cggatgagag tagaagagtt taattttata aagtgaaaag aggtaatgta cgatatacgt
     1201 agggctgaac caatagagga taagcaaacg atgttacagg cgcaaaattc atagcttact
     1261 gttttggaga taacagaaga ggaaataatg cagaagtaag gcgaaaaaag gacggaagcc
     1321 taacagcata tgaaataaag aaaaatatta taactactga taaatcggta ttgagcaaag
     1381 tactaatagg agtcaaaaac attcataagc tatgagtgtt tcttggctac ttttttattt
     1441 ttatgtatct acaaaagttt ccaggggcag aacctgtgat tattaatgga agaacctcaa
     1501 gtattgtctt aaatcattgt tatgaaaatg gattaaaaga gttcatcaag gtgtgattaa
     1561 caagttactt ttcattacat cgaatttttc agaagttgct tatattggtg atgatattaa
     1621 tgatttatca tgtatgacat ctgtgaagaa gcgggaggaa tagtagaatg tccaaaaaat
     1681 gccacaagaa atgagattga attatctgat tttattgtaa ctcacagtgg tggtgacgaa
     1741 tctgtgatag agtttattga gtggatattg gaatcgatat agtgtagtag tacagaaaaa
     1801 ttatcataca attaatacta ggcataagaa tattggcata ttctagagtt aaaagtaact
     1861 attcgaaaag gataaggtta gaattttttg ttttgccagt ggaagaatat aaaaagggca
     1921 ttggaaatta atatattaga atgctaagcg gagaaaaaaa tatatgaaaa gtacgtcctt
     1981 gtgatgggat ggaaaaatac acttatttca aacaacgata atataattat gtgaaccgaa
     2041 gtatactcgc ttctaattca atcctcaaat cccgacagtc gcgacttcaa aaaagcgtat
     2101 agttttaatg tatccccata tgatccgaaa aatggtaatt tacaattaac agtttgatga
     2161 tgttgaagat gtggaaatgc tgaaattacc atgatgagaa acacattaat taaagggaat
     2221 taattaacta gaatataatc tgattcaatt tgaattaagc aaaaaaaggc tgagttttaa
     2281 ataatcgcga attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa
     2341 ttgtatttcc gacgaaagaa aacaaaggag tgtaa
//
LOCUS       DACDXE010000165         2248 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.174, whole genome shotgun sequence.
ACCESSION   DACDXE010000165 DACDXE010000000
VERSION     DACDXE010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.174"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..193)
                     /locus_tag="IWM23_002948"
     CDS             complement(<1..193)
                     /locus_tag="IWM23_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885518.1"
                     /translation="MITFERIKELAKKQGKSLNKVEEDLGYGKNVLYRLKNSNPSTER
                     LQEIADYFDVSVDYLLGREE"
     gene            925..1182
                     /locus_tag="IWM23_002949"
     CDS             925..1182
                     /locus_tag="IWM23_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HAP9885519.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(1153..1461)
                     /locus_tag="IWM23_002950"
     CDS             complement(1153..1461)
                     /locus_tag="IWM23_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885520.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            1539..>2248
                     /locus_tag="IWM23_002951"
     CDS             1539..>2248
                     /locus_tag="IWM23_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9885521.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEG"
BASE COUNT          814 a          389 c          399 g          646 t
ORIGIN      
        1 tttcttcgcg ccccagaaga tagtctacag agacatcgaa atagtcagca atttcttgca
       61 aacgttctgt agaggggttt gagttcttaa gtctatataa gacgttttta ccatagccta
      121 ggtcttcttc aactttattt aatgattttc cctgtttttt cgctaactct tttattctct
      181 caaacgttat catatcaata ttcctttcgt tcaagaaaaa atatttaact ttatttgtta
      241 aaattcgttg actattttaa caaaaggtgt taatatcatt cttgtaaaca agtttaacaa
      301 ctaaaaagac aacaaaaaac actattgatt aataaatgcc aaccgccaag aaagctttta
      361 aaatcaatgt ttatatgtct tatttaacta tgttctgatt ttaacactgt atgttaattt
      421 agtcaacgca gttttttaaa aagttgttaa atttgtttac gaatataaaa gaaaggagag
      481 aagaatatgg aaaaaacagt ctcggcaaaa atcgaagatt tgaaattaga catactgaaa
      541 caagcaaaag tggcgatgga acacgcggta gataaagaag actctgccat ggttgcagcc
      601 atagcagaga ttttagctca cgtttagtca tcgtttttac ggtcttctgg aagctgacca
      661 tagataacag cataatgaat atctaaataa gcttgaacaa tttcttttgg cgaaattgat
      721 tcgcctttag tagatacttg tgattcgtga tttagccatg caacaactaa atccgcagcg
      781 atttcaaacg gcgttacact ttgtgtcagt tgccataaag aatttcacaa tatgtatggt
      841 cgtggtaata ataccaaaga gcaatttgag gaatttatgg aaattaaaaa atacaaaaaa
      901 tcatttatta aaggaggcta aacaatgaac aaggaaatga gccaaacaat caaggtacaa
      961 aaaatgatag atgatctaac acatggaatt gatagccaag ccgataaaat catcaaggaa
     1021 ttgcaaggac aaaaagttaa ggatgcaaaa atgcttctca agactatcaa ctttgaaatg
     1081 aatccaacaa aaagaaagct cgccgatgtg ttggaagaaa agttggcttc cgctatcaac
     1141 gagcaagaat tactatttga aaccgatact tttaacagct gatgttttat agatgacagt
     1201 ttcatccgaa tctatatcat cgccgagaga aaagaatgtt gcttttgaca aaaagccgat
     1261 aagacctaca agcggtcttg atgttgcaag agtagatacc ccaatatcac cttgcaaggt
     1321 attcacgtaa atttgagaag aatagaacat tccatctttt tcttcgatat ttgacgcatc
     1381 atcgtatcca acgaccaaag tatcggcaaa tattttgatt ttttctccat caagcatagt
     1441 aattacaaat tctggtttca taaattttca cctccttaac aattatttca gcctgtcaca
     1501 ctgataagga aattatacca aagaaaggaa tgagaaatat gaatacacca caaattttta
     1561 atttcgagca aaacgaagtt cgaactattt tagtaaatga tgaaccatat tttgtaggaa
     1621 aagatgtagc agacgttcta ggatattcga atccgcaaaa agctattcga gatcatgtgg
     1681 acttggagga taagacgcag aacgattcgt tcaccgtcaa tggaacagca gttgttttga
     1741 ttaacgagtc aggcctttac agtttaatct taaaatcaaa acttccctct gccaaaaaat
     1801 ttaaacgttg ggtaacgagt gaagtccttc caacaattag aaaaacaggt agctattcaa
     1861 acgtacctca aagttttgca caagcattgc gtttagcagc ggatttagaa gaaaagaacc
     1921 aattactcga acaacaaatt gccgagtacg aaccaaagat tagctactta gatacgattc
     1981 tttcatcgac agatacggta gctacttctc aaattgcagc tgattacgga atgtcggcaa
     2041 ttgctctaaa caaattgcta aacgagttag gtgttcagca taaagttagc ggacaatgga
     2101 tactttaccg aaaacatatg aaccaaggat acacaaaatc gcacacaagt gagataccga
     2161 aagccgatgg cggcactaaa gttgtaatga ataccaaatg gacacagaaa gggcgagtgt
     2221 ttatttacaa cttattaatc gcagaggg
//
LOCUS       DACDXE010000166         2231 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.175, whole genome shotgun sequence.
ACCESSION   DACDXE010000166 DACDXE010000000
VERSION     DACDXE010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.175"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..543
                     /locus_tag="IWM23_002952"
     CDS             <1..543
                     /locus_tag="IWM23_002952"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAP9885522.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(899..1471)
                     /locus_tag="IWM23_002953"
     CDS             complement(899..1471)
                     /locus_tag="IWM23_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885523.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1487..2092)
                     /locus_tag="IWM23_002954"
     CDS             complement(1487..2092)
                     /locus_tag="IWM23_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HAP9885524.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          700 a          453 c          338 g          740 t
ORIGIN      
        1 atttcaaaag agaatctctt aaatcaggac ttttctactg aaacgatctg tgagaaatgg
       61 gcagctgaca ttacgtacat tcctactaag aaaaatggtt ggtgttactt atcttcaatc
      121 atggatttac acacgaaaag aattattagt tatacatttt caaaacgaat gactgtggat
      181 tgtgtcattc aaacattgaa taaagcaaaa atacattatc acattccaga aggaatgatt
      241 ctacacactg acttgggcag ccaatacaca gcaagagaag tggaacaatg gcttaaaacc
      301 aacaaaataa ggcattctta tagtagaaaa ggaacacctt atgataatgc tggaatcgaa
      361 tctttccatg cctcattgaa aaaagaagaa gtctacacga ctagctactc agattttgaa
      421 gaagcaaatc gagcgctatt tagctacatt gaaggatttt ataaccgaaa tcgaatccat
      481 agctcgattc actatctaac cccacaggaa tttgaagaat tagcaaaaga aaaaatggct
      541 taaccgtctc tactaaagac aatcaacacc gtagggatat ggagataaaa tcaatagagt
      601 caatagatta tttatcgata ataacactaa aaaagccacg ttcctaaacg tgacttacgc
      661 tgaacgatat gtgttgtttg gtagacgaac aaatcgttca ttataaatat atgatggtat
      721 cctaatatag taagcgtggc tagcatacct ttaattactt taccttttac agttaccata
      781 cacatttttt gagcagaaat caagtaaata aaaaaatatt atatttcaca acatgctaat
      841 agactcatat ctagaacgca ttaagtattt acctgtctgg ttcgctgaga cttctttttt
      901 aactttcttc aattttcttc tttattctaa aaagcgtact gcgactgaaa ccggttatag
      961 cttcaacttc cttatacgat ttacctgaag taagcagttc atatgcgtgc ctcttcttcg
     1021 gtgtgattgt tgctttcgga cgtccttctc tgaaattagg attattcttt cttgcaaaca
     1081 tttttccttc ttgggtacgt tccacaatca tatcacgttc catttctgct acactcaaca
     1141 acgtccgtgt caccattcgc cccatggacg tattttcaat cgttcctaga ttcaaaacct
     1201 tgatcgttac gttatcatcg agtaaaggtt caatgatctc taatgcttct ctcgtgttac
     1261 gagcaaatcg atcaagcttc gtaacaacta tttcatctcc aggcttcaga ccagccaaca
     1321 gctgattgaa ttttggccga tcccgttttg tacccgtaaa tttttctgaa aaaatattct
     1381 tcttctctac gcagttctca agcaactgtt gaatttgttc ttttgtactt tgagaagcgg
     1441 ttgagacacg tgcatatcca tatttcgtca tttggtccac ctacttttac aaatcatcta
     1501 tcgtatactc agaataccct tcatagaaat agctgacatc gattcctttc atatataact
     1561 cgcgatcgtc aattttatca gtcagagcat tggaaattag atagcgtatt tccaaatcgt
     1621 ttatgggact tcgctccatt gcagaaagat agtctgcttt atcgattaag ttccaatcta
     1681 caactttttg caattcttcc tttaaaatga gatccagcca aattctagta cttcggccat
     1741 ttccttctcg aaatgggtga gcgatattca tctccacata tttcttgata atctcttcaa
     1801 aagaagtttg tggcatctga tcaatatgct gtaacgaatg ttctaaatac ataactggtg
     1861 caaatcgaaa atttcctttt gcaatattga ccgatcggat ttttccggca aaatcgtaaa
     1921 tatccgaaaa tagaaattca tgaatttcag ataaaccttt aaatgtacca acttcgattt
     1981 catttatttt tccacaatcg tacaattctt tcgctttttg tttactgagt ttttcttcaa
     2041 ccttagccaa ctcaacttga ttggttaatc ccaatttatt ctctaaaatc atgcgaaatc
     2101 tccttaactt tatgacataa gtttatgaaa cactctcaac cccattatat cgcgattttg
     2161 tttatgtgtc aaaaactatt aagtttctga cccccgatag atttttcaag tgtctctatt
     2221 ttgcacagtc t
//
LOCUS       DACDXE010000167         2207 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.176, whole genome shotgun sequence.
ACCESSION   DACDXE010000167 DACDXE010000000
VERSION     DACDXE010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.176"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(151..621)
                     /locus_tag="IWM23_002955"
     CDS             complement(151..621)
                     /locus_tag="IWM23_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885525.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            708..1406
                     /locus_tag="IWM23_002956"
     CDS             708..1406
                     /locus_tag="IWM23_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9885526.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1584..1925
                     /locus_tag="IWM23_002957"
     CDS             1584..1925
                     /locus_tag="IWM23_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885527.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1918..>2207
                     /locus_tag="IWM23_002958"
     CDS             1918..>2207
                     /locus_tag="IWM23_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9885528.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          806 a          315 c          446 g          640 t
ORIGIN      
        1 aggcaagcgt tgataggaac gattgtttac atggtttgga tttttatggg gcttgatgaa
       61 gctgaagctg agtacagagc gaaaaagcca accgaggggg ctgactaaaa atgaatagaa
      121 aagagaaact agaatgtata ttactattac tcagtttaat tctttcacta atttctctat
      181 tgggaagttt gtatttttga tatcaacaat tttgtggatt tgggttctat ttagagtaaa
      241 tatcattttt gtttggtgat taaggagttt attaaaatta attttcccta cttcaaatgc
      301 aaggtaacaa gatatagcac ttttaggagg aatatttagc ggtacagctt cagagtatat
      361 tctattatca tcgactgatc ctcctttagc aattaatttc ttaaatggag tagctgacca
      421 tctgcggttt aagttagcgg atgaaagttc taaattaact aacatagctg gttctgttga
      481 atagttagaa atgattactt ttgtataaat catatctgca gcgaagtaag aagcattaag
      541 ttcaacttct agttgagggc gcattccttt tatttttatc cctgttaaaa acgtactcaa
      601 tataaaactt attattgaca tccattgaaa aattgttaag tgaaaaaaat tcaaaataaa
      661 caccaccagt ttttaactaa attataccaa aaaggagaga agaaataatg caagaattag
      721 taattttgaa aaataaagaa gctgtgacta cgagcttaca agtggcagaa agcttcgaga
      781 aaaaacatca acatgtttta agagatattg atgcactaaa aaaagatgtg tccaattttg
      841 gacagatgtt tgtagaaggt aatgaaccag attcatatgg cagaaatcga cgagttttct
      901 tcattagtag agatggattt ttcttgctgg ctatggggtt tacaggaaag aaagctatct
      961 acttcaaaca aaaatacatt gaagcattca acgaaatgga agatgttatt cgcaagaata
     1021 ctgttcctca aacaattgaa gatatgatga tctatcaatt agaagaaatg aaagatgtta
     1081 aaaaagatgt ttccatgctt aaagatacta tgcgaattag cggacaacaa gagtttgaaa
     1141 ttaagcaaaa aggaaatatg aaagttatgg gagttctagg gggaaaagaa agccgagctt
     1201 atgaagaaat cagcaaaaaa gtattctcaa aattttggtc tgaatttaaa cgtacctttt
     1261 caatcccaag atatggcgag ttacctcgta agagattcga tgatgctgtt tcatttattg
     1321 aaatgtggtt gccagaaact gcgatccgta tggaaattga tcaactgaac agacaacaga
     1381 gactttttgg tgatgacaat gaatagagct gaagcgctaa gaatagggac ggcaattgct
     1441 aattgctggt ggaaatacta taaaccaatc atcctaagcc aacaacatat tgacaagcaa
     1501 aaagcatggc aacaaataaa aaagtgactc cgccggcaag caaagagtca caaagaaaac
     1561 acatcataag gagattttag catatggaaa aagaactttc cactctagat caatatttga
     1621 ttgatcctga ttggggcaag ccgaaaattg aggaaacaag tggtcgaaaa atcagacgaa
     1681 atcttttgac gaatgaagaa ctagcttggg atcaagatga tttaggcaac catgtaacta
     1741 tttgggatca tgtttatctt atccatctat cgaagcattc gaataaacct gaatatattt
     1801 acgtcatcga agatggcttg attgatgcgc tagaagagta cgacagagat aacttgattg
     1861 atatctctta ttacggacca ggtaagaaat acattgctga aatggaggca gaatttgatg
     1921 agtgaaatca aagggacaac gaactttgaa aaacttttta gtcgtaagtt aaataaaatt
     1981 ctcaagaaaa aaggaaattt tgattattta tcttgggctc acgcgtggga gattatgaaa
     2041 aagaatgatc cacaggcaac ggtaactatt aatgagtaca aacactacag ggttgtttct
     2101 ggaactcatc aagactttct tgttgaggaa tataaacctt ttcttatgga tgaaactggg
     2161 acttatgtat ctgtctcagt aacggttaaa ggacacacgg aaaccga
//
LOCUS       DACDXE010000168         2099 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.178, whole genome shotgun sequence.
ACCESSION   DACDXE010000168 DACDXE010000000
VERSION     DACDXE010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.178"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            169..1539
                     /locus_tag="IWM23_002959"
     CDS             169..1539
                     /locus_tag="IWM23_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HAP9885529.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="IWM23_002960"
     CDS             1560..1844
                     /locus_tag="IWM23_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885530.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          728 a          307 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttc
//
LOCUS       DACDXE010000169         2065 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.179, whole genome shotgun sequence.
ACCESSION   DACDXE010000169 DACDXE010000000
VERSION     DACDXE010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.179"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..492
                     /locus_tag="IWM23_002961"
                     /pseudo
     CDS             1..492
                     /locus_tag="IWM23_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            712..1008
                     /locus_tag="IWM23_002962"
                     /pseudo
     CDS             712..1008
                     /locus_tag="IWM23_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176405.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2479 domain-containing protein"
     gene            1171..1617
                     /locus_tag="IWM23_002963"
     CDS             1171..1617
                     /locus_tag="IWM23_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885531.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="IWM23_002964"
     CDS             1619..1756
                     /locus_tag="IWM23_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9885532.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="IWM23_002965"
     CDS             1794..>2065
                     /locus_tag="IWM23_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885533.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DACDXE010000170         2061 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.180, whole genome shotgun sequence.
ACCESSION   DACDXE010000170 DACDXE010000000
VERSION     DACDXE010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.180"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..337)
                     /locus_tag="IWM23_002966"
     CDS             complement(<1..337)
                     /locus_tag="IWM23_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9885534.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAK"
     gene            complement(404..559)
                     /locus_tag="IWM23_002967"
     CDS             complement(404..559)
                     /locus_tag="IWM23_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010001600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885535.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1176..1397
                     /locus_tag="IWM23_002968"
     CDS             1176..1397
                     /locus_tag="IWM23_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885536.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1398..1655
                     /locus_tag="IWM23_002969"
     CDS             1398..1655
                     /locus_tag="IWM23_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885537.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1791..>2061)
                     /locus_tag="IWM23_002970"
     CDS             complement(1791..>2061)
                     /locus_tag="IWM23_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HAP9885538.1"
                     /translation="KSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKD
                     LIDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          679 a          462 c          314 g          606 t
ORIGIN      
        1 ttttagcata ttgaaatttt tctggattat ccatatgagc aaaatatcgc accatacctt
       61 ttgcattagc acatttttgc gggtttggag accgaagctt ttgagttatc tcacgtattt
      121 gatcatacgt ctttttaccg ctaaacatta tcaaaacatg ccaatgaggc tttttcaatt
      181 caccgtcagg attcacatct ttatcatgaa gtggactctc aacccaagga acatgtaact
      241 catctaaaaa ctccctccaa ttttcaggag ccgattcagg atagactaca aacgtccaat
      301 tccttgtgcg ttcatcttta gtttttctac tcgtcatgct ataattacct taattgaaca
      361 acagtctaac agcaaaagca gtgcgccaac actaatgctt ttgctatttt ttttgctcat
      421 tttcctttcc gttagcatta acccaaattg taataagacc agacttacta agacccattt
      481 gtttcgcaat tttatcaaga ttatctgcca cttccttagt aagcgttaca acgactctct
      541 ttttttcatt actgatcata attcgtatcc tccaacgact ttttcttaaa aaccatagta
      601 acaaaaaagt aactaaggtg caacagttta gatactaatt agcgaataat aggccttaaa
      661 cccttggcat tactgatttt gtcaaaaaaa aacgctgaaa cccttgggag agtaaggcta
      721 aacagctatc gaccaatgac caatgaccaa taaggggggt gtcgtagtcc ccccctcctc
      781 cgcccaattc ataaacacca aaaccaaaaa acaaacctca gggctccgcc ccgaaccccg
      841 acctccttct accgaaatat atgcaggcgc cacgcttact atttcgttca gctatcggct
      901 tttgcaatct aacacgaccg tttcgctacc agtcgcctta caggctccat tctcgcttct
      961 cttacgtgtt ttaacgattg cgccgataac taaacgaaat agacgctaaa tcgcttaaaa
     1021 cgcaaaatag acccttttta gagcattgat tttttctact agtcatttcg ataatataaa
     1081 attgaccaca acacagaaaa cttatattat aagcttatgt cgtaagtccg acgtactcac
     1141 gtaatataga cggacgttaa atggaaggag cataaatgag agtgataaaa tcagaaaaat
     1201 tcactttggc cgaaattatg acgaccagcg aacttgcaca agaacttgat tacaaccaag
     1261 cttacgtttt gcgcttagcg aaagaacatc tgactgaagg aaaagaatat cgctctgccg
     1321 gtagaagaaa ctatcttttc agcccagaag cccttaaaaa actagaaaaa gtcataacca
     1381 ccaaagaagg tgagtaaatg cctaaagaaa caactatcaa cgttgaacaa ctagaaaaat
     1441 tgctagaatc atattccaat gattttatcg aattgaccgg cggaaaagtc gaagaaacca
     1501 ccgacctctt tgacctactt ctcacaaata ttctggaatt aaactctgaa gaacaaaaag
     1561 agcataaggg aaaactgata gcaccttcct acaggaaggt ggctaagtcg gctgtgccaa
     1621 ctggttttct tacaaaacaa ccttatattt tttgatatca caaaaaaatc cattttggac
     1681 ctattttcgg tcataatata gtacctactt ttggtcatag tttcgtccgt agttttggtc
     1741 atagtgacta agactttcac gcacaaaaaa agagctgaaa ttaacagctc ttatccttta
     1801 tttatttttg aatttacact tccaccatga cgattcgatt ttatatattg cccgataata
     1861 taagctgaat tatcacaaag aagcggaaac caatcatcaa atctttctga cattgcatag
     1921 tcaattaaat ctttgatttc agtgatattc ttagaatcaa caaacgacat catctctcta
     1981 atcaactcat atcgttctgc actcgttact gacaaataac tagcaatttc tgcaccacca
     2041 tgagcgataa tatcactttt a
//
LOCUS       DACDXE010000171         2002 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.181, whole genome shotgun sequence.
ACCESSION   DACDXE010000171 DACDXE010000000
VERSION     DACDXE010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.181"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..378
                     /locus_tag="IWM23_002971"
     CDS             <1..378
                     /locus_tag="IWM23_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAP9885539.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IWM23_002972"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IWM23_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAP9885540.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2002
                     /locus_tag="IWM23_002973"
     CDS             1859..>2002
                     /locus_tag="IWM23_002973"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAP9885541.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          554 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tt
//
LOCUS       DACDXE010000172         1986 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.182, whole genome shotgun sequence.
ACCESSION   DACDXE010000172 DACDXE010000000
VERSION     DACDXE010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.182"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..597
                     /locus_tag="IWM23_002974"
     CDS             <1..597
                     /locus_tag="IWM23_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9885542.1"
                     /translation="SANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGF
                     TGSKAINFKLKFIEAFNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMR
                     ISGQQEFEIKQKGNMKVMEVLGGKESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPR
                     KRFDDAVSFIEMWLPETAIRMEIDQLNRQQRLFGDENE"
     gene            773..1114
                     /locus_tag="IWM23_002975"
     CDS             773..1114
                     /locus_tag="IWM23_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885543.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWGHVYLIHLWKQPRKPEYIYVIEDGLIDALEEYDRDNLIDISYYGQGKK
                     YIAEMEAEFDE"
     gene            1107..1853
                     /locus_tag="IWM23_002976"
     CDS             1107..1853
                     /locus_tag="IWM23_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9885544.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPQDVSMLEPASQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
BASE COUNT          743 a          311 c          438 g          494 t
ORIGIN      
        1 tcggccaatg ttgaagatgg gtccaatttt ggacagatgt ttgtggaagg gaacgagcca
       61 gactcatacg gaagaagtcg gagagtttat ttcatgaata gagacggatt ttccttgcta
      121 gctatgggat tcactggaag taaagcaata aatttcaaac taaaatttat tgaagctttc
      181 aacgaaatgg aagatgttat tcggaagaat actgttcctc aaacaattga agacatgatg
      241 atctatcaat tagaagaaat gaaagatgtt aaaaaagatg tttccatgct taaagatact
      301 atgcgaatta gcggacaaca agagtttgaa attaagcaaa aaggaaatat gaaagttatg
      361 gaagttctag ggggaaaaga aagccgagct tatgaagaaa tcagcaaaaa agtattctca
      421 aaattttggt ctgaatttaa acgtaccttt tcaatcccaa gatatggcga gttacctcgt
      481 aagagattcg atgatgctgt ttcatttatt gaaatgtggt taccagaaac tgcgatccgc
      541 atggaaatcg atcaactgaa cagacaacaa agacttttcg gtgatgaaaa tgaatagagc
      601 tgaagcgcta aaaataggcg taataattgc taatcgctgg tggagacaca ataaaccaaa
      661 catcctaagc caacaacata ttgataagca aaaagcatgg caacaaataa aaagcgactc
      721 cgccggcaag cattgagtcg caaacaaaat acatctaagg agatgttacc acatgaaaaa
      781 agaactttcc actctagatc aatatttgac tgatcctagt tggggcaaat cgaatatcaa
      841 ggaaacaagc aatcgaaaaa tcagacgtaa tcttttgacg gatgaagaac tagcatgtga
      901 tcaagacgat ttgggaaatt ttgtgagtat ttggggacat gtctacctta tccatctatg
      961 gaagcagcca agaaaacctg aatacatcta tgtcatcgaa gatggcttga ttgatgcact
     1021 agaagagtac gacagagata acttgattga tatctcttat tacggacaag gcaaaaaata
     1081 catcgctgaa atggaggcag aatttgatga gtgaaaagga acaacctttg aaaaatagaa
     1141 gtgataatac gctttttaat accttataca aaataaatgt gaaagatgtt actgaaaaac
     1201 gaaataacct cacttatctt tcgtgggcat gggcttgggc agaagttagc aaagtgtgtg
     1261 aggcagtaga ttatgaaatc tatcatgatc cagaaacata tctgccgtat gtctttgata
     1321 agaaaacagg gtacatggtt tttaccagta tcacagtcaa cggagtaaag cgtgacatgt
     1381 ggttaccagt catggatggt gcaaacaagg caatgaaaga tgagccatat acctacgaag
     1441 tcaatgatta tcagtggaat aacgaaacga agaaaaaaga gattgttgga aaaatcgaaa
     1501 agcgagttga agcagcaact atgtttgata tcaacaaaac gatcatgcgc tgtcttgtaa
     1561 aaaatctagc aatgtttggg ctagggctat atatatttgc tggcgaagat atgccacaag
     1621 atgtctcgat gcttgaacca gctagccaaa gaagcaaaaa gctattctta gatgctttac
     1681 aactggttgc taacaagtac gaaaaatcga ttgatgaagc aattgttgca ttgactgatg
     1741 cagcttccat aaccgccgat gacagtaaat ggaccaagag agacttgggc attctaaaac
     1801 gaggcgttaa ctggattgaa gatcagtaca gagaagaaac aaaagagaag tgatatgagt
     1861 gtttaaacca ttaatcgatt catattcagc ggttctgaaa aagttcaaag gaaaagacat
     1921 aggtgcaacg atcaatgaag aagtgaacat tgatcgacta aagacgatgt atgacggcta
     1981 cgatgg
//
LOCUS       DACDXE010000173         1977 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.183, whole genome shotgun sequence.
ACCESSION   DACDXE010000173 DACDXE010000000
VERSION     DACDXE010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.183"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..223)
                     /locus_tag="IWM23_002977"
     CDS             complement(<1..223)
                     /locus_tag="IWM23_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885545.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLA"
     gene            537..1151
                     /locus_tag="IWM23_002978"
     CDS             537..1151
                     /locus_tag="IWM23_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAP9885546.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            complement(1175..1525)
                     /locus_tag="IWM23_002979"
     CDS             complement(1175..1525)
                     /locus_tag="IWM23_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9885547.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(1519..1782)
                     /locus_tag="IWM23_002980"
     CDS             complement(1519..1782)
                     /locus_tag="IWM23_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAP9885548.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
BASE COUNT          674 a          339 c          296 g          668 t
ORIGIN      
        1 gagcaagtaa tggagaaact attaaataat tttcagtaac agttaagtcg ttagttgggt
       61 taccaatggc tattccgttc ctatctaaag gagttaattc ttggttatta tctactacag
      121 tagttggttc atttgtacta tcagcaaatg taggaattgt agttccagtt acactaattc
      181 caatagttaa aatacctaaa gataaaataa acttattttt cattgtctat cacctcgaaa
      241 tcaaaaataa gaaaacgttt tcttaataaa tcttaacaga taaatcaagt tatatactag
      301 aaaattcata aaatatattt ttaatgtcat aatcgtttaa attttcttga ttgacattta
      361 tttacaaaaa atattgccat ttaaaaagaa aagtgacaca tactatttcc tccatcatta
      421 gtttttgtct atataaggta taccctaact agacacgacc gcgacattga aaaatctggt
      481 tttctcgtgt atgattaggg tgttttttaa atagttgcag aaagtaggta aaaattttga
      541 gagttgcata catacgtgtc tcatccatcg atcaaaatga acaacggcag attgaagaaa
      601 tgaaaaaatt cggtgcagag agaattttca ttgagaaaca atctggcgca acgattactc
      661 atcgacctgt ttttcaagaa gcactggatt tcgtccgaga tcaagatatt tttattgtgg
      721 aagctattga tcgacttgga agaaattacg acgagatcat cgcttcagtc aattacttga
      781 agaagaaaaa tgtgcagctg atcatcacaa gtctaccgat catggccgaa gcaattggca
      841 atcctctcct ggataaattc attaaagatt tgatcattca aattttggcc atgatcgctg
      901 aacaagaacg taccgaatct aaacgacgtc aggcacaagg cattaagatt gcaaaagcga
      961 acggtgttta taaaggacgt cctaagctat acagtgccaa tgcaaaagat ccccaacgtc
     1021 gtcttgttta taaaaacatc gttgaggatc taaaaaaagg tgttgcgatc gctaagattg
     1081 caaaagacta caatgttacg agacaaaccg tatatagaat aaaaaaagac agcatggtca
     1141 atcacgaata gttgatcatg ctgctgtttc tattttattt gcttactttt tgaaaaactg
     1201 ttctaacttt catcaataac tcctcaacta aactatcatg cgctttttcc aaaaaaatac
     1261 actctctggc ttcataatca attgtcgtta gttgatctaa taatactttt cctgttgtct
     1321 tcatttcata ttctgagttt atatttacat atagcggata gtttctagtt gtactagaaa
     1381 ttggagcaac tgtaacaacg ttactataat ccgcaataat atcatgactt aaaacaatat
     1441 aaggtctttt acctgtctgt tcgtgtccag atcgaggtgc tgtattcaat aatagaatat
     1501 cgccttgtct aggttttctc accatttttc attacctact gcttcaccta aattttgaat
     1561 cgtcacatga tttctttcat cagtgtaatt ttcaaataac tgttcaattg ttaattcatt
     1621 ttctatatta gccgcttcga taattagccg cttctgaccg tttacttctt cttgtattat
     1681 cttcactttg tcttctgtat gaattccaag atattccata atatactttg gcaatctaag
     1741 gccattacta tttccccatt tttttatagg taacatttcc atagtagaca cctctttctg
     1801 tatatacata gtataacata cataagaaaa aacgtatata cttaattatc cttttctatt
     1861 aaattagatt aacattccat gaatccataa actatgttaa catttatatg aaaatagtcg
     1921 aaaggaaatc ataatatgtt taagaaaaca aaatctttag aatcaggtaa gaaaaaa
//
LOCUS       DACDXE010000174         1962 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.184, whole genome shotgun sequence.
ACCESSION   DACDXE010000174 DACDXE010000000
VERSION     DACDXE010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.184"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..272)
                     /locus_tag="IWM23_002981"
     CDS             complement(<1..272)
                     /locus_tag="IWM23_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885549.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="IWM23_002982"
     CDS             complement(309..446)
                     /locus_tag="IWM23_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAP9885550.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="IWM23_002983"
     CDS             complement(448..894)
                     /locus_tag="IWM23_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885551.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(1342..1962)
                     /locus_tag="IWM23_002984"
                     /pseudo
     CDS             complement(1342..1962)
                     /locus_tag="IWM23_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          509 a          407 c          342 g          704 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aa
//
LOCUS       DACDXE010000175         1880 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.185, whole genome shotgun sequence.
ACCESSION   DACDXE010000175 DACDXE010000000
VERSION     DACDXE010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.185"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..995)
                     /locus_tag="IWM23_002985"
     CDS             complement(<1..995)
                     /locus_tag="IWM23_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAP9885552.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="IWM23_002986"
     CDS             complement(1013..1567)
                     /gene="srlA"
                     /locus_tag="IWM23_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAP9885553.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            complement(1555..>1880)
                     /locus_tag="IWM23_002987"
     CDS             complement(1555..>1880)
                     /locus_tag="IWM23_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885554.1"
                     /translation="VMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYH
                     PSVRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSD
                     KRWIS"
BASE COUNT          542 a          423 c          317 g          598 t
ORIGIN      
        1 cagctatata atccaaaact tgctacccaa gcaatgataa ctcgtgggat gccgttaaga
       61 aatcttgaat aaagaactga tggtacacct acttctactg tctcagcttt tgcttcttcc
      121 aaacctagac cgactggaat aaaatcacat gcattttgag tattgatagc aaatagtgcc
      181 ggtaatgcta attggggagg aatatttcct ttcccaatct ctacaccgat caatgttcca
      241 atcacttggc taataactgc ccctggacca agcaaaggag ataaaaatgg cagtgaacaa
      301 ataaagccaa tgattgttaa tccaactgca tttcctgcta aaggtgccat aatgttagca
      361 aatacattac cgatcccaga accttgtatc acaccaatca aaagggaaac gaaagccata
      421 aaagggatca ctgtccggat catcgtatct actgcttctc ttgctgattg attgaatgta
      481 gcaactactt ttcccgcgcc cattccaatt tttgctatcc agttttgctg ctctgctttt
      541 tgctcggtta tctttttaga agtatcatat ttgtactttt tatctggaga tgcagtattt
      601 actgtttctt tgttatcatc agtttccatt agtggctgta tttgttctaa ccctactgct
      661 gaaacataaa tatcttcagt gatatattgt gctaatgggc cactttttcc tgtgggaacg
      721 atattgatgg ttggtatccc ttttttagga taaattccac atctaagagt ccctccacag
      781 tcaacgattg caagcgcgat ttcactatca ggtattgatg ttttgaaccc attgatagac
      841 tccatacctg tcagttcttc tattttatct acgatcattg gcctctcacc tccacctgtg
      901 acatagatga acttgtgttt atcttttgtc ggcctaataa tcaaaggacc tccatacccg
      961 ccgtttcctc ttacgatctt aatatcacca tacatttaaa actctccttt ctctaatcaa
     1021 ctgaaagctc tacttttttg ctaagaacga ttccttgctg tctgcataca aatccagtag
     1081 taaaatcagt tatccaacca ccaataaagt tcatcagcag tcccacaagc aagtatcgga
     1141 tagccagctc catcgaattt agccctaagg tttcgatccc ttgagcaatc cccatccata
     1201 caaacaattc tccaggatta atatggggga atacaccatt gctcgtatga caaaattgag
     1261 cttgagcagc ataatagctt ggtttgtagt attctggaag gaaacgagcc attgtaaatg
     1321 acataggatt tcccaacata aaagcagata agaacggtaa cacaaggtaa cgagtaagcg
     1381 gatttttcgc tgataatttg gctactttcg ttactcggtc ttcacctaaa aaagcaatca
     1441 gcgtattcat tgcaaccatc agcatcaaga caactggtac gattcctgtc atccagctta
     1501 tgaatgtttc tgcacctgtt tggaacagcc tcataaatcc ctcagcaaat tttgttatga
     1561 tatccatcgt ttgtcactcc ttttttcttt gatcgtactt ttccatatcc gaaaattatt
     1621 gagcatagca ataaaaggca atgaaggttg ttcacttttt tcatttcctg cctctattcg
     1681 taagtatact tctctggcat tttctaaagc tgcttgtgtc aaacgatctt ccgctcgaac
     1741 tgagggatgg taacgatcga tgtaatggat atcttcaccc gcatagacag gtaatgaatg
     1801 aaattttgcc aaagatgtaa ccccttgcat ttttctgact tctaaaactt gatcatgatc
     1861 gtctatagct agcataacaa
//
LOCUS       DACDXE010000176         1833 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.186, whole genome shotgun sequence.
ACCESSION   DACDXE010000176 DACDXE010000000
VERSION     DACDXE010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.186"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..969
                     /locus_tag="IWM23_002988"
     CDS             <1..969
                     /locus_tag="IWM23_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAP9885555.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
     gene            1159..1232
                     /locus_tag="IWM23_002989"
     tRNA            1159..1232
                     /locus_tag="IWM23_002989"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1193..1195,aa:Ile,seq:cat)
BASE COUNT          605 a          317 c          346 g          565 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccata aatagtcaca
     1081 ctccaagcta tgcgataaca ggtttgttgc cacacattct actggttgat tgtttatggc
     1141 tttatgtggc aacaaccagt acccttagct cagttggtca gagcagacgg ctcataaccg
     1201 tccggtcgta ggttcgagtc ctacagggta cattaacgta gccatttgaa tcgttctgtg
     1261 ttagaatttt tttgaagagt attatacaag ctaaagcttt tcttcattgc cactcaaatg
     1321 agtggctttt ttatgtatcc ttttatggat taatgaaagg atgtttcaca tagttatact
     1381 tctgtatatt tgaaaagttt tactttgatt tttaaataga aagacatttg ggttaaattg
     1441 tgagataata ataaagaaga gtttaaagcg caccccaaac cacttcccca taagtgtgtt
     1501 acgctttaaa ctcttttata tttgaagcca ttaaaaagca taccatattt ttgaaaaaaa
     1561 gtgagaaaaa aggcttacaa ttggagtggt agttaattag tgacttattt ttgattttat
     1621 agcactgata ctataaaata tagatatcat catattacac aatcttaata ctaacttaaa
     1681 aaatatctcc tttcacaagt atggtgataa aattcgttcc gggctacctt tttaggtagc
     1741 ctactttaat ctttatacct ttctggatca acgaaagtat actttatata gtcataacgc
     1801 cgatgatcgc tacgtgcgtc cggcacgtca gtc
//
LOCUS       DACDXE010000177         1799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.187, whole genome shotgun sequence.
ACCESSION   DACDXE010000177 DACDXE010000000
VERSION     DACDXE010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.187"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..619
                     /locus_tag="IWM23_002990"
     CDS             <1..619
                     /locus_tag="IWM23_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAP9885556.1"
                     /translation="VDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQ
                     YRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGR
                     GKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQK
                     KRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            640..1173
                     /locus_tag="IWM23_002991"
     CDS             640..1173
                     /locus_tag="IWM23_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAP9885557.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
BASE COUNT          769 a          308 c          281 g          441 t
ORIGIN      
        1 cgtagatata cgaatttacg aaaaagcact gcctttgtta aataatttag acgagtgggt
       61 acgctattct ttacaacaat tcaacgagct acaaagtggc tatgcaaaga ctatgtttcg
      121 tctgttaaag caatacagaa ccaaaggttt cgcatatttc tccaaagaag actttcttga
      181 attattagat attcctaaaa gttataagca acctgatata gataaacgag ttataaaacc
      241 tatacgtcaa gaactaactg caatcttcaa aggactaaca atcaaaaaga aatacggaaa
      301 aggacgggga aaacctgtta ttggctatca atttacgttc aaacctgaga tcaaaaaagc
      361 agacgacttt aacaaacatg caccaaccaa agaagaccaa aataaagcat tggctttagc
      421 tgataaacaa aaagaatacg aagaacccgc tacaacaatt aacgaaaaaa tcaaacagaa
      481 gaaacgagaa cttgaactcc tccaacaaat caaacaacta gaaaaagaga aaaaagctct
      541 tgaaaaacaa acagagtccg cactttcgga aaaagagaaa aaaagtttat tatctgattt
      601 agataattta ttccagtagc aacctaggag gcaaccagta tgcaaccaca agaaaccttt
      661 acaataaagg aattgtcaga tctttttaag atgagtaggc aagcaatatc gaaacatatc
      721 caaaaattag attcttccat gatagctaaa aatgagcgtg gttataaggt tgtattgcgt
      781 tctggagtat tgcaacttgc tcgcaactta gacaatcaaa ggttgctaga taagttgcat
      841 aaacatgaac aaaacaacga taaaaatgct tatataccaa acaaaaacgt ggttgcaaac
      901 aagttgctag atcagttgcg tgaagaaaat gaatttttac gagaacaact tgccctcaaa
      961 gagcaaaata attctcatat acaaaaacta ctcgaccagc aacaacaact caccctgcaa
     1021 gccaaccaac aaatcgaaaa acttcaggag caactgcagt tgacttatac ggaagagtct
     1081 tcagagaata gttcaacgac tctttctgaa gcaacagaaa caatggacga acaaacaaag
     1141 aattggaaaa aatggtggca gctatggaaa tgaagaagga aatacttatg acaaaaaaac
     1201 acttgctatt cggttctatc ctactaggaa tattaagctt tagcccaatg ttattatcag
     1261 tggccaacaa aggaaataaa aaaggggaac taaaactcaa tcctaattac tacaacagtc
     1321 aaaatgagga agtatttgta gaaaaacttg ataaataaga aaagactgct cgtacgagca
     1381 gtctttctga aagagattat cctattatta accttagaaa agaataataa atgaatatga
     1441 gatatgtaaa aagcataaca aacacaaaca aaaataacaa gtatttaaaa aatgaccgat
     1501 caatattcac aaagaattag aaagaaacaa ccgcagaaaa ccaagcctta aaaagagctt
     1561 ttaacctaga ccaataaaaa ttcacaaaat cagcgcctgg atcccttggt acaggcgtta
     1621 cttggtagta tacacattat gtctactagc tgtttaaata actgatctta taattttatt
     1681 taaacgatta tgaattcaaa actacttttt atgacgttaa tcattgttat ataataaaat
     1741 gttatataat aaaattacta agattattta atataacaag caattttaaa aaaacgaaa
//
LOCUS       DACDXE010000178         1796 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.188, whole genome shotgun sequence.
ACCESSION   DACDXE010000178 DACDXE010000000
VERSION     DACDXE010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.188"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            29..1381
                     /gene="brnQ"
                     /locus_tag="IWM23_002992"
     CDS             29..1381
                     /gene="brnQ"
                     /locus_tag="IWM23_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAP9885558.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT          503 a          309 c          353 g          631 t
ORIGIN      
        1 ttttaaaaaa accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct
       61 ttttataggc tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt
      121 tcatttgggt caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac
      181 cggtatcggt cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt
      241 atatgaatta gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta
      301 cttagttatc ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg
      361 tttagcacca tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt
      421 attttttgtg ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg
      481 aaagttcctg aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt
      541 gaatccacta ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt
      601 tttcacaggg tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg
      661 gatcatcatt gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa
      721 agacacgatc aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct
      781 cgcttattta ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac
      841 gctggctcag attgctgatc attatttagg tacttatggc agtatcttat tagcatttat
      901 cgttatatta gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt
      961 cacagatttg tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc
     1021 ttgtttagcg gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat
     1081 gttcatctat ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa
     1141 gcagcgtacg gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga
     1201 tgggttgaac gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata
     1261 tgttgcggaa agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt
     1321 tatcggtttt gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg
     1381 ataaaagagg ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat
     1441 ggcttctcac atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg
     1501 tttattgaga ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa
     1561 atggcttctc acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac
     1621 cgtttattga gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct
     1681 ccatatactt tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt
     1741 tcttttataa tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACDXE010000179         1792 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.189, whole genome shotgun sequence.
ACCESSION   DACDXE010000179 DACDXE010000000
VERSION     DACDXE010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.189"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            132..344
                     /locus_tag="IWM23_002993"
     CDS             132..344
                     /locus_tag="IWM23_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885559.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..1024
                     /locus_tag="IWM23_002994"
     CDS             506..1024
                     /locus_tag="IWM23_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HAP9885560.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVLDILNMYRDITFSKNKLKDINSIDNYYIQFKGFDFNSSTEF
                     KLALYAQFFIEKLNRFQEIVEDSDFNTFNSHKPMRGTYIKFLENYNDLKSTNNYQFGN
                     LSYEMISKVLSL"
     gene            1031..1543
                     /locus_tag="IWM23_002995"
     CDS             1031..1543
                     /locus_tag="IWM23_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885561.1"
                     /translation="MKLNSINDLFAIIGVIANILFSCLNFRFTQKNKKNKLSIEQMKK
                     DIETDLKNDHKERRKIEEYTSLMEKCKATDAIFSFLRKSDEIITRENIDLENEISKLE
                     SIFSDINSLKYLIVEENSDSFLEFEKYLTDLIDNRNNELMRQNIARFLTKLVSEWKSI
                     ISQNMDKLRY"
BASE COUNT          681 a          221 c          280 g          610 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct agacatatta aatatgtata gagatattac
      721 ttttagtaaa aataaattaa aagatataaa ttcaattgat aattattata tacaatttaa
      781 aggttttgac tttaatagct ctaccgaatt taagttagca ctttatgcac aattttttat
      841 tgaaaaatta aataggtttc aagaaatagt tgaagatagt gattttaaca cttttaatag
      901 ccataaaccg atgagaggta cttatataaa atttctcgaa aattataatg atttaaaatc
      961 taccaataat tatcaatttg gaaacttgtc ttatgagatg ataagtaaag ttctaagttt
     1021 gtgatgataa atgaaattaa atagtataaa tgatttattt gctattatag gtgttattgc
     1081 taacatactt ttttcgtgtt tgaattttag atttactcaa aagaacaaaa agaacaaact
     1141 cagtatagaa caaatgaaaa aagacattga aactgattta aaaaatgacc ataaagaaag
     1201 aagaaaaatt gaagaatata cttctttaat ggaaaagtgt aaagcaactg atgcaatttt
     1261 tagcttttta agaaaaagtg atgagataat cacacgtgaa aatatagatt tagaaaatga
     1321 gattagtaaa cttgaatcta tcttttccga tataaattct cttaagtatt taatcgtcga
     1381 agaaaattcg gatagctttt tagaatttga aaaatatcta acagatctta tagataatag
     1441 aaataatgaa ttaatgagac aaaacattgc aagattcctt actaaattag tttctgaatg
     1501 gaaaagtatt atttctcaaa atatggataa attaagatat taaggatgtt agttttaaaa
     1561 taaactaatg tccttatttt tgtgttcaca tttctgttac ctaagaaata cttaaaggag
     1621 gtgatgtagc cagcaacgac tacaacttga acccgctatc tacagtttat ttgttgtttc
     1681 ttttgggtat actctaatgg tacaaatcca tatttgctta aagcgttgat ttttcagcgg
     1741 tttttcttta ttgcaaagtg taccctaaga cctgtatact aatagtaaat ag
//
LOCUS       DACDXE010000180         1772 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.192, whole genome shotgun sequence.
ACCESSION   DACDXE010000180 DACDXE010000000
VERSION     DACDXE010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.192"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            197..424
                     /locus_tag="IWM23_002996"
     CDS             197..424
                     /locus_tag="IWM23_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAP9885562.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            547..873
                     /locus_tag="IWM23_002997"
     CDS             547..873
                     /locus_tag="IWM23_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAP9885563.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            941..1654
                     /locus_tag="IWM23_002998"
     CDS             941..1654
                     /locus_tag="IWM23_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAP9885564.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
BASE COUNT          686 a          273 c          374 g          439 t
ORIGIN      
        1 aacaaagtag ttgacacttc cgctacttag tattagtacg aaagtatact tcatgatact
       61 aagtagataa gaagtgaaaa gcggaaccca cgatcgtcaa aatgctaact gctttgctta
      121 caccagataa agggaagagc aatatcgctt attttaggcg tggagaagca agcaaatccc
      181 tacgatcaca aaaaaaatgc tgacccagca gttaagagag ttagaacaag atgaaagtat
      241 ccatcgaaaa gcctataacc aagtcccgcc taaagtagag tactcgctca ccgaagaagg
      301 aaaatcatta agagagattc tagtagcgtt gtctgtttgg gaagaagaac gtatcgaaaa
      361 acaaaaagcc tcaagggaag aagacacgct actgcataca catggtggct acctaaatta
      421 ttgattttct atgacattta ttttaaataa aagaaaacag gagttttttt aaaagcatca
      481 tctatttttt atgtaaaaaa atggtatgat taatcaagta agaagtgatt ggaagaaggc
      541 aagcaaatga atagtatgga aaaagtcaac cagtttagag atgaacgtaa ttggcgacaa
      601 tttcataacg aaaaagattt ggctatctct atttctttgg aagcatctga attgttagaa
      661 ctttttcaat ggaaaacgcc ggaagaagta aaagaaacac aattagaacg aatcaaagaa
      721 gaactggcag atgtattgat ctattcatac atgatggcag acaaccttca cctggatcta
      781 gatgagatca ttgaagaaaa attgatcaaa aataatttga aatatcctgt agaaaaaagc
      841 gcaggtgtac gaaaaaaata cacagagttg tagaaatgtg tcattcggct gttggaaaga
      901 gcaaaagaaa aacagtaaaa tgagtctgga gggagagaaa atgaaaaaaa aaggaaaagc
      961 aaaaagatgg ctagtcaata tcctcttgtt cctcttgtta ttagtaggac tagctttgat
     1021 tttcaatgaa cagatcaagg attattttgt cagagagaca ggagataaat atgctatagc
     1081 gaatgtaaca aaagaagatc tgaaaaagaa taacgataaa gatgtcagtt ttgactttga
     1141 tgcggtagaa ccaatgacga cagaaggggt catgaggtcg caaatgagag gaacagatct
     1201 accagtgatt gctagtatcg cagtcccttc agtttcaatc aatttaccgg ttttcaaagg
     1261 cttggataat acgtcactac tttacggagc aggaacatta tctccagatc aagaaatggg
     1321 aaaaggaaat tatgccttag caagccatcg tgcaacgaac cctgaattgc tgtttacacc
     1381 gctagaaaat ttagagatgg gtgccaagat ctatttgaca gatttagaaa atgtgtatac
     1441 atacaaaaca ttttttaaag aaaaagttgc tcctacagat acccaattac tgaatgaagt
     1501 cgagggaaaa gaaattgtca cactaattac ttgtggcgac atggacgccg tcacgcgact
     1561 agttgtacaa ggagaactgg aaagtgttac atcgataaaa gatgcgacag atgatatgag
     1621 atctgctttc aatctagaga caaaaacatt ttaggatact gggacataag tcgttacaga
     1681 ttaaaaacag ccaaaggata tgaatcgata aggaaaaacc aatttgctga aaaaaagaga
     1741 aacgcaaaga ggtgcatctc tgatttggtt gt
//
LOCUS       DACDXE010000181         1741 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.193, whole genome shotgun sequence.
ACCESSION   DACDXE010000181 DACDXE010000000
VERSION     DACDXE010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.193"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="IWM23_002999"
                     /pseudo
     CDS             1..1228
                     /gene="aph(2'')-I"
                     /locus_tag="IWM23_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1212..1604
                     /locus_tag="IWM23_003000"
     CDS             1212..1604
                     /locus_tag="IWM23_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885565.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            1605..>1741
                     /locus_tag="IWM23_003001"
     CDS             1605..>1741
                     /locus_tag="IWM23_003001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HAP9885566.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     S"
BASE COUNT          717 a          133 c          298 g          593 t
ORIGIN      
        1 aaaaaaacat tatacagagc cttgggaaga tgaagttttt agagtaatta ttgaatataa
       61 caatgttcct attggatatg gacaaatata taaaatgtat gatgagttat atactgatta
      121 tcattatcca aaaactgatg agatagtcta tggtatggat caatttatag gagagccaaa
      181 ttattggagt aaaggaattg gtacaagata tattaaattg atttttgaat ttttgaaaaa
      241 agaaagaaat gctaatgcag ttattttaga ccctcataaa aataatccaa gagcaataag
      301 ggcataccaa aaatctggtt ttagaattat tgaagatttg ccagaacatg aattacacga
      361 gggcaaaaaa gaagattgtt atttaatgga atatagatat gatgataatg ccacaaatgt
      421 taaggcaatg aaatatttaa ttgagcatta ctttgataat ttcaaagtag atagtattga
      481 aataatcggt agtggttatg atagtgtggc atatttagtt aataatgaat acatttttaa
      541 aacaaaattt agtactaata agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt
      601 tttaaataca aatttagaaa ctaatgtaaa aattcctaat attgaatatt cgtatattag
      661 tgatgaatta tctatactag gttataaaga aattaaagga acttttttaa caccagaaat
      721 ttattctact atgtcagaag aagaacaaaa tttgttaaaa cgagatattg ccagtttttt
      781 aagacaaatg cacggtttag attatacaga tattagtgaa tgtactattg ataataaaca
      841 aaatgtatta gaagagtata tattgttgcg tgaaactatt tataatgatt taactgatat
      901 agaaaaagat tatatagaaa gttttatgga aagactaaat gcaacaacag tttttgaggg
      961 taaaaagtgt ttatgccata atgattttag ttgtaatcat ctattgttag atggcaataa
     1021 tagattaact ggaataattg attttggaga ttctggaatt atagatgaat attgtgattt
     1081 tatatactta cttgaagata gtgaagaaga aataggaaca aattttggag aagatatatt
     1141 aagaatgtat ggaaatatag atattgagaa agcaaaagaa tatcaagata tagttgaaga
     1201 atattatcct attgaaacta ttgtttattg atgataatat ggtttttgat attgataatt
     1261 taaaaggatt tcttaatgat accagttcat ttgggtttat agctaaagaa aataataaaa
     1321 ttataggatt tgcatattgc tatacacttt taagacctga tggaaaaaca atgttttatt
     1381 tacactcaat aggaatgtta cctaactatc aagacaaagg ttatggttca aaattattat
     1441 cttttattaa ggaatattct aaagagattg gttgttctga aatgttttta ataactgata
     1501 aaggtaatcc tagagcttgc catgtatatg aaaaattagg tggtaaaaat gattataaag
     1561 atgaaatagt atatgtatat gattatgaaa aaggtgataa ataaatgaat atagttgaaa
     1621 atgaaatatg tataagaact ttaatagatg atgattttcc tttgatgtta aaatggttaa
     1681 ctgatgaaag agtattagaa ttttatgatg gtagagataa aaaatataca ttagaatcat
     1741 t
//
LOCUS       DACDXE010000182         1712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.194, whole genome shotgun sequence.
ACCESSION   DACDXE010000182 DACDXE010000000
VERSION     DACDXE010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.194"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(134..1282)
                     /gene="tnpB"
                     /locus_tag="IWM23_003002"
     CDS             complement(134..1282)
                     /gene="tnpB"
                     /locus_tag="IWM23_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAP9885567.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
     gene            complement(1299..1703)
                     /locus_tag="IWM23_003003"
     CDS             complement(1299..1703)
                     /locus_tag="IWM23_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HAP9885568.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          504 a          295 c          273 g          640 t
ORIGIN      
        1 tgacggttct taacgcttga agtgggagct tcttgggaat agagcatact tgatagcgta
       61 tttctttact aacagcggtg tctcttattt accaagctat ccccgtagtt cctacggttc
      121 ttgatgattg gatctaagcc aatcccatag aatgtagtct tagaccttcg gtcagaatat
      181 tgatactagc attgatatca cgatcatgat gagtatgaca aacaggacaa gtccattctc
      241 gaatttcaag agatttcttg ccatctttat gtccgcactc tgaacaaatc tgactagacg
      301 gaaaccattt atctactttg atgatttttc gtccatacca gtcagccttg tattgtaatt
      361 tagtcacaaa ctttgaccaa gaaacatcag aaatactttt agctaattta tgatttcgca
      421 acataccttt tgtgtttaag tcttcaatac agataatatc gtgatttttg atcatttctg
      481 tactcaactt attcagaaaa tcagtacgtt gattcattac tttttcatgc aatctagcta
      541 ctttgcgttt ttgtttttga taatttttag cttcaaatag attggtacct ttatttttag
      601 ccaataaagc acgcctagac aatttgcgtt gttcacgctt tagtttcttt tccattttgg
      661 acgtgaattt attattatca attttttgtc catcagaaag aatcgcaaag tccataatac
      721 ctaagtcaat accaattgtg gaattagttt taggtaattc gataatttct tctttacaca
      781 acaaagaaat atagtattta ccgctagaat gacgtgatat tgtagcggat ttgataatcc
      841 cttttggttg tctatgaagc ttaattctta ctaatgactt caatttagga actttgatga
      901 atttactatc aatcaaagca actgtaccat tttgattatt cgttgtataa ctctgaacag
      961 gatttttctt acttttgaaa cgtggaaatc caacggattt atcccgaaaa aaattcttgt
     1021 atgctttatc taaattaagt tgggcattgg ctaaagcaag gctatcaatt tctttcaaaa
     1081 acggaaattc tttcttgtat ttagctggtg tcggaaaagt cattttttta gaagaatcat
     1141 ttttaacttc ttcatacgcc ttttttcggt catcaagcat tagattgtag accttacgga
     1201 cacaaccaaa agatttggca aagaagattt cttgttcctc tgttggatag attctgaatt
     1261 tgtatgcttt tagttgttcc ataaagttca cctactttct atttatgatt gtttccttga
     1321 ttttggatat acttcttaat cacatcaatt ggtgcgccac cagttgtcag aagacaaaaa
     1381 cttttagacc agaacatttc tttccaaaga aattgtttga ctcttggaaa gtcacgtttt
     1441 atcaatcttg agctggcact tttataggca ttgatgaatt ttgtcaattc tgttttaggt
     1501 tgagctttga acataatatg aacgtggtct ttatcatgat tccactccac taaagtaatg
     1561 tgatacgatt ctgaaattct ttcaaaagta gttttggcat aatcagatat ttcatcatca
     1621 atcacttgtc tacgatattt cgttactaaa acaaggtggt aataaagaag aaatactgaa
     1681 tgattatttg tatctaattc catgttatat ca
//
LOCUS       DACDXE010000183         1639 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.195, whole genome shotgun sequence.
ACCESSION   DACDXE010000183 DACDXE010000000
VERSION     DACDXE010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.195"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..153
                     /locus_tag="IWM23_003004"
     CDS             <1..153
                     /locus_tag="IWM23_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9885569.1"
                     /translation="NKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFSFSVSTEIKV
                     LMGITA"
     gene            159..575
                     /locus_tag="IWM23_003005"
     CDS             159..575
                     /locus_tag="IWM23_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885570.1"
                     /translation="MEFREGAFDFQTSQQNRKFIPEDFQNLQLENMRLEDLFRLADDD
                     KYRFEVLKIKKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNR
                     NSTDVVMITDIYVEDEVIIKYMKPVIKLVNKNKGNG"
     gene            681..944
                     /locus_tag="IWM23_003006"
     CDS             681..944
                     /locus_tag="IWM23_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAP9885571.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            944..1216
                     /locus_tag="IWM23_003007"
     CDS             944..1216
                     /locus_tag="IWM23_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAP9885572.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            1293..1622
                     /locus_tag="IWM23_003008"
     CDS             1293..1622
                     /locus_tag="IWM23_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9885573.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          625 a          207 c          324 g          483 t
ORIGIN      
        1 aataaatttt atcaaagtct ccgtacagcc tcttccacga ttaagggcat ggagaccatt
       61 cgaggaatat ataaaaagaa ccgaagaaat ggaacgctct tcagcttttc ggtgtctact
      121 gaaatcaagg tattaatggg aataacagcc taagatattt ggagttcaga gagggcgcgt
      181 ttgattttca aacttcgcaa cagaaccgaa aatttatacc ggaagatttt caaaacttac
      241 agttagaaaa catgcgttta gaagatttat ttagattagc agatgatgat aagtatagat
      301 ttgaagtttt gaaaattaaa aagaatgaac tattgaataa gttatatcct gagaatgcga
      361 taaaagcaca ttttggtgat gctaatcata tgtatagttg gttcattcct aaaagattta
      421 gagggaaaat caatgctgga gatcaagtga aagtactcaa tcgaaatagt acagatgtcg
      481 taatgataac agatatctac gtagaagatg aagttataat caaatatatg aagcctgtga
      541 taaaacttgt taataaaaat aaaggaaacg gataacactt tacaatgctg ttttggtact
      601 ccaatttaaa caaaacaatc gaatttacgt acttaattaa gtacgttaga atggagatat
      661 aaattagagg gggagtagat atggcaaaaa cagtcttaaa tcctagctca gcacggaaaa
      721 atttttataa gttgttgaaa gatgtaaatg agaatcatac ggaaattgaa atcattagtg
      781 agcgcagtga aaataatgcg gtgcttattg gtttggatga ttggaaagca atcaaagaaa
      841 cgctattact tgagcaaata ggcactttgg gtgttgttcg taagcgagaa aaagatgata
      901 gtggatttac taatgttgat gaaattgatt gggataactt ataatgaata actattctgt
      961 gatgattaaa aactcggcta aagctgattt aaagaaaatc aaacagtcta atttgaaacc
     1021 tcaatttgag aaggttattc agactttaaa ggaagatccc tatctcccta cccaatcatt
     1081 tgagaagtta aaacctacac atgaaggtag gtattctaga agattgaata gacaacaccg
     1141 ggttgtttat aaggtagatg aagatgaaca cgtggttgaa atttattcag caaggacgca
     1201 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
     1261 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
     1321 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
     1381 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
     1441 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
     1501 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
     1561 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
     1621 gaaatcgttc cttcaaaac
//
LOCUS       DACDXE010000184         1609 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.196, whole genome shotgun sequence.
ACCESSION   DACDXE010000184 DACDXE010000000
VERSION     DACDXE010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.196"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1609
                     /locus_tag="IWM23_003009"
     CDS             <1..>1609
                     /locus_tag="IWM23_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAP9885574.1"
                     /translation="GEIVTLELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVVDQIRM
                     DLGDGVNSNHPIYLELARLCEQSPEKEQEQTVKNEPISKVQNEIKETKTKQKGYQKRT
                     AEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQFPGAT
                     ACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVRETEATPEDKKRIESGQ
                     IEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDQTGQTELQVGIQAVAKN
                     IGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNTPTQHLATSIHELAHA
                     RMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYIATWTKNSQTLDDKEAAER
                     GKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQEHKNTVETAEQKNIVPVGS
                     LWIDKKDGKVLEEDTGRTLHLKDAAFIEDPQHNQVILKGKSYGKEIQTALPSEEFALG
                     NPFRFSLETVSEVAKRKNEIYYEQQLSQ"
BASE COUNT          617 a          284 c          351 g          357 t
ORIGIN      
        1 tggggagatc gtaacactag aattgatcga tcaactcaaa acactggata accaatacgc
       61 cgcagaagca ggctattata aattctattt tgaagaagtg aagaatggag aagtagtcga
      121 tcagatacga atggatctag gggatggcgt caatagtaat caccccatct atttagagtt
      181 agctagattg tgcgaacaat cacctgaaaa ggaacaagaa caaacagtca aaaatgaacc
      241 tatttcaaaa gtacaaaacg agattaaaga gacaaaaaca aaacaaaagg gttatcaaaa
      301 acgaacggca gaagagatta agcaagaagt caaatcactt tcccaacagg cgcttgatgc
      361 ggtgaaaaag catactcatt cacctgaaga cgtcaaagaa cttttgaatt ttatgtctcg
      421 ttttccagaa cgatccttta gaaatcaatt attgatcgag gaacaatttc caggcgcaac
      481 ggcttgttta ggtcgtgccg cgttgaaaaa agaaggaatc tatatcaaaa aaggagaaca
      541 agggaacaag atctttgtca gaaaaacggt caaaggattt tatgagaaag gacgtggctt
      601 tgttcgagaa acagaggcta caccagaaga taaaaaacga atcgaaagtg gtcaaatcga
      661 agtgatcaaa aaaccttact acacgattga aaaagtgttt gatatcacac aaacacaatt
      721 gaaacctgaa gactatccga aaatcttccc gaaccgcgtc tttgattttc aattggatca
      781 aacagggcag acagaacttc aagttgggat tcaagcggta gccaaaaata ttggaattca
      841 aattcgagat atgacagaaa gtgaagtcta tcaaagagaa ttaggacaag cacgtggggc
      901 gtacgttcgt aacgaattta ctggcgaaga agaaatcgtc atgaatactc gaaatactcc
      961 tacgcagcat ttagccacaa gtattcacga attagcccat gctagaatgc acaaattttc
     1021 agaattagat actgcaacaa aagaactaca ggcagaaatg acctcctata ttgtgtgcaa
     1081 gcattttggg atggacacct cggagaaagc tattccttat attgcgacat ggacgaagaa
     1141 tagtcagacg ttggatgata aggaagctgc ggaacgtggg aaaatcatga atgatgtcag
     1201 tcgtgtcgca aatgaattca ttcaaaccat ttctaatgaa atcaatcaat acagagaaag
     1261 agaaccagag atccagccag cagaagtgaa gcaggagcat aaaaatactg tagaaacggc
     1321 agaacaaaag aatattgtgc ccgttggctc actttggatc gacaagaaag acgggaaagt
     1381 cctggaagaa gacacgggcc gaacgttaca tttgaaggat gcggcgttta ttgaagatcc
     1441 gcagcataat caagtgattt taaaaggaaa aagttatgga aaagagatcc aaacggcgtt
     1501 accttcagaa gaatttgcgc taggaaatcc attccgattt tcacttgaga cagtgtcaga
     1561 agtggcaaaa cgaaaaaatg aaatctatta tgaacaacaa ttgtcacaa
//
LOCUS       DACDXE010000185         1547 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.197, whole genome shotgun sequence.
ACCESSION   DACDXE010000185 DACDXE010000000
VERSION     DACDXE010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.197"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            96..506
                     /locus_tag="IWM23_003010"
     CDS             96..506
                     /locus_tag="IWM23_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAP9885575.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     TLAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            520..876
                     /locus_tag="IWM23_003011"
     CDS             520..876
                     /locus_tag="IWM23_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAP9885576.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVEKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          566 a          229 c          284 g          468 t
ORIGIN      
        1 ttattttgat ttttttttat attttattag ctaataattg gtattttaaa agaaattact
       61 gtagaattgg attataatta aaaaggagtg agaaaatgat aaaagtatat acttcgccta
      121 gctgtacttc ctgtagaaaa gcaagagcat ggttgcaagc gaatcaactt gaatttgaag
      181 agaagaatat ttttgccgaa ccgttaacag aaagtgagat caaaaagacc ttggcagcga
      241 ctgaaggagg agttcaagat attatttcca ctcggtcgaa aatttacgaa aagctgggca
      301 tagattttaa cgaattaact ttaaagcaaa tgatcgtctt attcaaagaa tatccttcat
      361 tattgagacg acctattttg attgatgaaa aaagaatact aatcggattt aatgaagatg
      421 agatccggtc atttgtccct cgtgaaattc gatgtgttgc cagaaatctg atctatttac
      481 aaaacaataa catcttaaat tcatagggag ggagccttcg tggaagtgaa agtctatgga
      541 tcagcgcact gtgtaacaag tctgaaggcg aaacaatggt tagaacacca tcaattgcct
      601 tatcaatttg ttgacttaga agaacgcgat ctaagtacag aagaagcaga agaattatgc
      661 agttttgaag aagtgcaagc agaacagcta tttgcgactt ggtctgaggc ttttcacaac
      721 attgcgattg acctttcctg tgtagaaaaa aaacaactga tttttttatg cacacaacat
      781 accaacctcc tcagacgtcc tttgattatt atcgatgaag ttctatttat tggatacaat
      841 gagcagttga tggaacagct tatcttaaat aaatagactt taactgtttt aatcaagtca
      901 ctgagcattc catacagttg taacagtcga acacttagca tcaagcgtgt tatgtgttct
      961 ttttttgact ttatttttct actatagaga catgctcaat ttatgtggtc aaaattcaaa
     1021 tctaggtggg gatatagagt taaaaatata ttgacatgtg taaatttaca tgatatattc
     1081 atttctgttg ttttaagtag caaagcaata aaatagtatt ttaaaaaaca tataaaatag
     1141 ttgttgacat gttagaaatg acatgataag ataactgagt cgttaaaaaa ggaccgcttg
     1201 cgaagcttga ttctatcgaa aaaacttcaa aaaagttctt gacaaataac aaacgacaag
     1261 ttatgataaa tgagttgctg taagatttcg aaaaacgaaa tcaaaaaaac ttttaaaaaa
     1321 gagttgacaa ttacttgtct ctctgatatg atataaaagt tgctgcggca gccaaaaacg
     1381 aatcgggaaa cactcctgat gaagtagacc tttgaaaaat gaacaaagta agacaaacca
     1441 aatgtgtagg gcgtcttgat tcaattcaag acaacaaaca tttttaacaa gcaagcaata
     1501 tgctagcaaa caaattgagc ttaacaatcg caaggttgtt cgaactt
//
LOCUS       DACDXE010000186         1522 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.198, whole genome shotgun sequence.
ACCESSION   DACDXE010000186 DACDXE010000000
VERSION     DACDXE010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.198"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            316..570
                     /locus_tag="IWM23_003012"
     CDS             316..570
                     /locus_tag="IWM23_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9885577.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            571..888
                     /locus_tag="IWM23_003013"
     CDS             571..888
                     /locus_tag="IWM23_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9885578.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          536 a          232 c          295 g          459 t
ORIGIN      
        1 acaaaataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac ggtaaccctc
       61 ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc taattctgtt
      121 aaatggatgt taacggaagt gtatggtgag tagttcactc gtattttgat atcgtttgta
      181 tatgttttca gcgttctaag tggcttattt tgtccaataa taatatggag tggaatccct
      241 taagaaacac cgttggcaat tgttgttata acatgttata atttattcat caaaaactaa
      301 aaggagtgtg ataacatggg aaaagaacta aaggttagaa agatcggcaa tagcgtggga
      361 gtgatccttc caagctctct tggattaaaa agcggagaca ttatccaagc caaacaagaa
      421 gacaacttat tcatattaga tactactcaa atagcaaaag aacacgatcg caaattgatt
      481 gaagaaagtt tccaagactt tgagaaaggt ttaacagttt cagaaattga aatggtaaaa
      541 gctttcggaa aatacggctg gagtgaatag atgaaagagt acacgctagt ttattctaaa
      601 agttttcaga ccagccttga aacgataata gaagaatggg aaaatgagct tgctctttct
      661 ccagccagca taaaaagatt tgtaggtgcg atccagaagg ctttagaact aaccaaaaat
      721 tttccaacta tgcatgagga agtttccgaa atctatggaa tggatatccc aacatatcgg
      781 atcgtgatcg gcaaaagcta tgccttattt tatcggatcg accatgaaaa aaaagagata
      841 cttgtaggac gtattttcca atctaaacaa atgaaactag gcttttaaca ataattagga
      901 aaaagtcgct catcctctac aggtgggtga ttaatgccgc tcgatatggt tgttaaccgt
      961 cggttactcg aagattgata tataagactt agtacctttt tattttgctc catgttatgg
     1021 ccgaaggtct aaagatacaa tcattagtct agagaaaaat taagaacctt aggaactacg
     1081 gggatcgctt ggtgaataag agacactgct attagtgaaa aaataagata tcaagtacgc
     1141 tctattccca aaaatttcgt gactttactc atgagaggtt caagtagtat atttttaatc
     1201 aaaattgacg taacttttaa taaatactac ctaaataagg tagtatttat ttttgtgttt
     1261 taaaagagga agtccaatat atctatggga aacttcttaa atattatgtt ggtacatgaa
     1321 aatatacttc cctgctagac tatttacaac attttacttt gtacgtattc ttgttttaaa
     1381 aggaatggga taaaatatag gtacaataac tatttgatgc taaatataat taatggagga
     1441 gagatagtga acatacttaa tgaaaaaaga tatgtatggt ttgctggtaa aaaatatatt
     1501 aacccgtggt gctagttaat tt
//
LOCUS       DACDXE010000187         1515 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.199, whole genome shotgun sequence.
ACCESSION   DACDXE010000187 DACDXE010000000
VERSION     DACDXE010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.199"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..354
                     /locus_tag="IWM23_003014"
     CDS             <1..354
                     /locus_tag="IWM23_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAP9885579.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IWM23_003015"
     CDS             355..903
                     /locus_tag="IWM23_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885580.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1163..1515
                     /locus_tag="IWM23_003016"
                     /pseudo
     CDS             1163..1515
                     /locus_tag="IWM23_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          598 a          184 c          326 g          407 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcgtga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag gaggagatgt aggtgaaaaa gtttaaaaag aaaatatatt taggattggt
     1201 tgttggggtg agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa
     1261 ccaaaaagaa aggattatga gccaattaga tgactctcaa ataagtatgt acccaatgag
     1321 ctaattgtca gtttaaaggc tacacaaaga atttcaacag agaaaaataa accgcaaaat
     1381 ttattgggtg taaaagttat ggtggaaaaa gatttgactc ttaggataaa tgaaagcgat
     1441 tataataagc gagatcaagt attattagtg aaattgaacg aagaaattag tgtggaagag
     1501 gcagtaagaa cattt
//
LOCUS       DACDXE010000188         1501 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.201, whole genome shotgun sequence.
ACCESSION   DACDXE010000188 DACDXE010000000
VERSION     DACDXE010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.201"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..125)
                     /locus_tag="IWM23_003017"
     CDS             complement(<1..125)
                     /locus_tag="IWM23_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HAP9885581.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYG"
     gene            complement(143..463)
                     /locus_tag="IWM23_003018"
     CDS             complement(143..463)
                     /locus_tag="IWM23_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885582.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            762..>1501
                     /locus_tag="IWM23_003019"
     CDS             762..>1501
                     /locus_tag="IWM23_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAP9885583.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEG"
BASE COUNT          557 a          258 c          234 g          452 t
ORIGIN      
        1 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
       61 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
      121 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
      181 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
      241 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
      301 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
      361 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
      421 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
      481 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
      541 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
      601 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
      661 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
      721 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
      781 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
      841 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
      901 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
      961 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
     1021 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
     1081 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
     1141 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
     1201 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
     1261 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
     1321 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
     1381 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
     1441 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
     1501 g
//
LOCUS       DACDXE010000189         1453 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.202, whole genome shotgun sequence.
ACCESSION   DACDXE010000189 DACDXE010000000
VERSION     DACDXE010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.202"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..625)
                     /locus_tag="IWM23_003020"
     CDS             complement(<1..625)
                     /locus_tag="IWM23_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAP9885584.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="IWM23_003021"
     CDS             complement(784..1050)
                     /locus_tag="IWM23_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885585.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="IWM23_003022"
     CDS             complement(1040..1396)
                     /locus_tag="IWM23_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9885586.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          406 a          279 c          181 g          587 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattgatt ttatattata gtatataaca
     1441 taaaaattcg aat
//
LOCUS       DACDXE010000190         1451 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.203, whole genome shotgun sequence.
ACCESSION   DACDXE010000190 DACDXE010000000
VERSION     DACDXE010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.203"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..1406
                     /locus_tag="IWM23_003023"
     CDS             105..1406
                     /locus_tag="IWM23_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9885587.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          476 a          293 c          264 g          418 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
     1441 ctcactctat c
//
LOCUS       DACDXE010000191         1424 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.204, whole genome shotgun sequence.
ACCESSION   DACDXE010000191 DACDXE010000000
VERSION     DACDXE010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.204"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(150..1337)
                     /locus_tag="IWM23_003024"
     CDS             complement(150..1337)
                     /locus_tag="IWM23_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAP9885588.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACDXE010000192         1360 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.206, whole genome shotgun sequence.
ACCESSION   DACDXE010000192 DACDXE010000000
VERSION     DACDXE010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.206"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1359
                     /locus_tag="IWM23_003025"
     CDS             <1..1359
                     /locus_tag="IWM23_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAP9885589.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          453 a          244 c          290 g          373 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa
//
LOCUS       DACDXE010000193         1355 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.207, whole genome shotgun sequence.
ACCESSION   DACDXE010000193 DACDXE010000000
VERSION     DACDXE010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.207"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1355
                     /locus_tag="IWM23_003026"
     rRNA            <1..>1355
                     /locus_tag="IWM23_003026"
                     /product="23S ribosomal RNA"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DACDXE010000194         1344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.209, whole genome shotgun sequence.
ACCESSION   DACDXE010000194 DACDXE010000000
VERSION     DACDXE010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.209"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(742..1083)
                     /locus_tag="IWM23_003027"
     CDS             complement(742..1083)
                     /locus_tag="IWM23_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAP9885590.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(1083..1313)
                     /locus_tag="IWM23_003028"
     CDS             complement(1083..1313)
                     /locus_tag="IWM23_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAP9885591.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
BASE COUNT          412 a          224 c          197 g          511 t
ORIGIN      
        1 tgtattgata gtgtacctaa aagctctact gacataagat cgtgtaatcg gtaaagtaat
       61 tttattaatt atacgggatg taatcaatta gagaataaac taatcataaa aggtactttt
      121 acattcataa ttgtcgcttt gagattctac tttggttcac tatctgaccc aattgcacat
      181 tttcgatcat tacaagtatt ggtgtcgtta gaacaactaa ataaaaacta gcggtagatt
      241 tttcagaatc tatgctaatt tctgtatgga tgttccagtg atggcaaatt tctcttttca
      301 ttgtttttct tgtcgcatga tatgcctttt tgtgttattc agttaaacta tagaaattct
      361 ttcctagaca gatcttgatc ccaaatttta cgtttttgag catcgatata gtttaaaatg
      421 acattactca tatctaaaga gtttagattt tttaggacta aaactgtcag ttgttctttt
      481 tcttctttct gttaaagtat tgtcgcgact tttcgtatac gttaagatat gtcgtgaaaa
      541 aacaagtttt gtgacacaaa aaaacgaatg acttgaggaa atcacctcaa tcattcgttt
      601 aaaatgctat atattcttta gtttaagata cggaaattaa tgccatcgac cacactagta
      661 gaatgaacga catttacttg gccttttttt aatttcgtta tgtttagcat ataaaaatat
      721 tatagagttt acttacaatg cttaaaacat atactgtata atctgatcaa ttttagccat
      781 gtcttgcaat ggtaagtttt ctattttttt gagttttcgt tccttaaaat caagagattt
      841 taattgtgta ataattactt gtccttttat atctagctct tcaggtaatg agtaacgtgt
      901 cggtaaattc tttatagtgg aagtaattgg acatatgacc gaaaacattg ttttacggtt
      961 aaattcataa cgagagacga ctagtcctgg tcgtcttttt tgaatttctt ttcctacgga
     1021 gggatcaaaa tcaatccata caatatcacc ttttttagga atataaattc tttcttctga
     1081 cactataaaa tttctctccc ttcaggagtt aaatcttccc attcgtcttt ctcataatac
     1141 tcggcctctt ccccaccact aaatggatct tcaatttttg gaactaacgt aattgttcca
     1201 tcatcagaat acacaacgat atattcttgg ttttctgaag gtttctttcc gttatcagaa
     1261 ggtaaagtta ccactacaga acttccttgt aatctagatt ttgtaacaag cattatcatc
     1321 actcccttaa taaaagtata ctat
//
LOCUS       DACDXE010000195         1314 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.210, whole genome shotgun sequence.
ACCESSION   DACDXE010000195 DACDXE010000000
VERSION     DACDXE010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.210"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..848
                     /locus_tag="IWM23_003029"
     CDS             <1..848
                     /locus_tag="IWM23_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885592.1"
                     /translation="EIADLIEKMYGHHYTPQTMSNITKSFTEEVTAFKGRELHDRYAA
                     IYMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQER
                     GLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRNISHKVRVNNRKEVCDDFKM
                     VYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLI
                     ESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLS
                     QPMEN"
BASE COUNT          429 a          240 c          254 g          391 t
ORIGIN      
        1 ctgaaattgc tgatttgatc gaaaaaatgt acggtcatca ctatactcca caaaccatgt
       61 ccaatataac aaaatcattt acagaagagg taacggcgtt taaagggcga gagcttcatg
      121 accgttatgc tgctatttat atggacgcaa cgtatattcc gttaaagcgg aaaaccgtcg
      181 ccaaggaagc tattcatatc gcagttggca ttcgcccgga cggatcaaag gaagtattga
      241 gctatgcgat tgcaccgact gaatccatca cgatttggga ggaaatttta ttagaccttc
      301 aagagcgcgg tttgaaaaat gtcctcctgt tcatcacgga tggcttaaag gggatggtag
      361 gagcgattag tcggttctat cccaaagctc gttttcaaca ttgttgtgta cacgtttttc
      421 gtaatatcag tcacaaagtg cgtgtcaata atcgtaagga agtctgtgat gattttaaaa
      481 tggtgtatca agcctcatct aaagaggtgg cgttagaagc acgtggtgct tttgcgaaaa
      541 aatggaaaac cagctatcca aaagtggttg aatcgattct ttcgaacgat cacttgctca
      601 ctttctatga tttccccttg gccatacgca agagtattta ttctacgaac ttgattgaat
      661 cctttaataa gcaaatcaag aaatacagcc accgcaagga acagttccaa aacgaagagt
      721 cgatggaacg tttcttagtc tcgtcttttg atacttacaa ccaaaaattc ctaggtcgca
      781 gtcataaagg tttccaacag gccgaaggcg agctcgaaca aatgctgagt caaccgatgg
      841 agaattagaa acaatctaca gtagggaaga accatttaca caaaattatt gacgctccca
      901 agctaaaact cctattaatc gatctactaa aaataaataa aaaaattctt ttcaattttt
      961 atttatggta tgacataaat aaagtaaagt tattcttcta ggttaatctt attaatactc
     1021 tttctcacta gtgttgcatg caacactagt gagaaagagt attaaatata ctgcaaggca
     1081 agacatttgg taattgaaga ataacaagca aaaaaatagc agtcaggtct cttgacaaag
     1141 tatagttgct attctttttt taggccctac tttaatgaaa tttttgtttg gagggaacga
     1201 cttcattcct ttgattattt cagttgaatt taatagagaa gcctggcgtc tattaagaaa
     1261 tacttttctt gatgagcgtc aattttttta acggaaatct atcctatact aaat
//
LOCUS       DACDXE010000196         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.211, whole genome shotgun sequence.
ACCESSION   DACDXE010000196 DACDXE010000000
VERSION     DACDXE010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.211"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..>1296
                     /locus_tag="IWM23_003030"
     CDS             46..>1296
                     /locus_tag="IWM23_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAP9885593.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          401 a          258 c          251 g          386 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt
      481 gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta
      541 tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg
      601 gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa
      661 cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat
      721 atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc
      781 gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa
      841 accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag
      901 cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg
      961 cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct
     1021 tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa
     1081 agagatcagt cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc
     1141 tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac
     1201 tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc
     1261 aatgcgtacg gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DACDXE010000197         1296 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.212, whole genome shotgun sequence.
ACCESSION   DACDXE010000197 DACDXE010000000
VERSION     DACDXE010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.212"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            141..398
                     /locus_tag="IWM23_003031"
     CDS             141..398
                     /locus_tag="IWM23_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885594.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IWM23_003032"
     CDS             complement(630..1043)
                     /locus_tag="IWM23_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAP9885595.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          489 a          165 c          162 g          480 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatacc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaa
//
LOCUS       DACDXE010000198         1271 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.213, whole genome shotgun sequence.
ACCESSION   DACDXE010000198 DACDXE010000000
VERSION     DACDXE010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.213"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            265..957
                     /locus_tag="IWM23_003033"
     CDS             265..957
                     /locus_tag="IWM23_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAP9885596.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          554 a          144 c          183 g          390 t
ORIGIN      
        1 aattattgac acaacctatt ctggaaactt acaaataaaa gcttattctt cagccacctt
       61 cgggtggtta ttttttataa aaaaatgtaa aaaaatattg atacataaat aaattagttg
      121 tagtaaatag ttttagaatt gaatttctct atccttttta aataattttt tagacaaaaa
      181 acagaaagtg tctaaagaaa gcttgacgaa gaaaataatt tttaataact tatttaagga
      241 aattttttga aggagaagat aaaagtgaga aaaaagatta ttattaatgc tggagtaatt
      301 ataggattga tgttagtaat tttctccgga aagcaatatg ctgataatat tttattatgg
      361 ggaggggaag ataatattga aaagatcaac aacaatttaa ctatattaga cacagctcta
      421 gaaaataaag agcaaaaaat tagtatttta aattcacaat tgtcttcaaa caaccaaact
      481 cttgaacaac taagaactaa tattgaagtt tccaaacaaa aagtggcatc tttagaaaac
      541 gacaagaatc aattagtatc agagaaaact aatttagaaa atcaattgtc agcaaaagac
      601 tctgaattgc aaggaaaaca aaatgaaatt aatgcgaaaa tagacgaaat taaccaaaaa
      661 gtagctgaaa taaatcgatt aaatagcgat tggaataatc gtttacaaaa tgaaaagaac
      721 aaatttaatg aagaacaatc tactattaat agtttaaacc agcaaattaa tcaaatgttg
      781 agtgaaaaaa ataacattca aaatcaactg caagctactc agcaaaagct agaagaagaa
      841 aagtcaaaaa atagtagttt gaataattac attgaaaagc ttcaaaaagc taaaaatgat
      901 gtagaggata ccgcaaataa agcacaacaa attgtaaatg aacatacaat ccaatagata
      961 tagttttaat ttaacaaatg gtatgtttgg agataatgag atagaatata aatgtcataa
     1021 tattatacaa tctaaacacc gtttcctttt ctatttttga tgacatcagt atatagctac
     1081 tcgaaagagt agtcttttta ttattctcgc ttattattga tatgaaaatt tcgatttgac
     1141 aaaaggtacc gaataatttt gtaatatgaa ttattatcaa gaagtaatta tgatgataac
     1201 atatggaaac tagttagtaa taataacagg tgattaaact tttgaacgac ttgtaattct
     1261 atattttttc t
//
LOCUS       DACDXE010000199         1269 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.214, whole genome shotgun sequence.
ACCESSION   DACDXE010000199 DACDXE010000000
VERSION     DACDXE010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.214"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWM23_003034"
     CDS             24..1103
                     /locus_tag="IWM23_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885597.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT          378 a          302 c          310 g          279 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaacccatga taacacagtt tcacgcaagg
       61 gaaaacatct ttcctactca gaacgttgcc aaatcgctat cctgaaaaag gaaggctatt
      121 ccaaccgtca ggttgccaaa gtgctgggac gtgttccgca aaccataaac aatgagattg
      181 atcgcggaac catcacccag ttaaaacgcc aaacgcaaaa tggaaaagtg tatgacacct
      241 actattctgt ttatgacgct gatgcaggac aggccttcta tgaaaagcaa cgactgaact
      301 gtggcagacg ccccaaatgg gcgaacacaa atgccttcat tgattgggca gatgacaaaa
      361 tgttaaatga aaaatggtcg ccggacgtcg tggtcggatt tgccagaaaa cacgagctgt
      421 tcgattgtgc catcattcct ggcacaaccg ctctttatca atggattgac cgcggaatca
      481 tgcgaacaaa gaacatggac ttgcttgaaa aactgtcccg taaaatcaaa gcggtccgcc
      541 caggacaccg tcccaacaag cgtgttctcg ggacgtcaat tgaagaacgc cctaaagaag
      601 ttgaaacacg ggaaacgttc ggtcattggg aaatcgacac ggtagtggga aacaagacca
      661 agtcggatgc cgtgctgctg acgctggctg aacggcagac acgctttgaa gtcatcctaa
      721 agttgaacgg gaaggacacc gaatcggtgg accaagcggt tcaacagcta agagaacggg
      781 ctggcgctaa cttcgatcaa ttatttaaga ccgttacatc cgacaatggc tcggagtttt
      841 ctgggctaca cgatgctctg caagatgtag ctgacgtcta tttcagccac ccctatgctt
      901 cttgggagcg tgggacgagc gaaaaccagc acaaattaat tcgccgtttc gtccccaagg
      961 gaagcccgat tagtgaggtg agcgagaacc agattttgcg gatccagcgt tggatgaacg
     1021 attacccgcg gaaaattttg ggttatgcga caccacatga tacattcgtc caggcattca
     1081 agcaagaacg gttggtggct tgacccatta ttccttgata acgatcctca ctcgggacat
     1141 ggcttcatgt ctggaaagct ggcctggggc cagaatcgtc tgaagactat catacctcag
     1201 ttcattatca aggcctcact gatttagaat aacgaggcag ttacggtatc aaacaaatga
     1261 gttacctgt
//
LOCUS       DACDXE010000200         1265 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.215, whole genome shotgun sequence.
ACCESSION   DACDXE010000200 DACDXE010000000
VERSION     DACDXE010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.215"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..1103
                     /locus_tag="IWM23_003035"
     CDS             24..1103
                     /locus_tag="IWM23_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885598.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          335 a          343 c          356 g          231 t
ORIGIN      
        1 tcaatagaca aggatgaatt cacatgacgc aaaaccatga taacacagtt tcacgcaagg
       61 gaaaacacct aacctactca gaacgttgcc aaatcgccat cctgaaaaag gaaaactatt
      121 ccaaccgtca ggttgcggat gtcctgggct gcgtgccgca gaccataaac aacgaaatca
      181 aacgcgggac cgttactcag cttaaccgcc agaatcagaa cgggaaggtc tacgactatt
      241 atcatgacgt ctacgacgcc gatgccgggc aggcggccta cgagaagaac cgcttgaact
      301 gcggcagacg gcccttatgg gccgacacgg acgccttcat tgaatgggcg gacgacatga
      361 tgctgttgga aaaatggtcc ccggacgtgg tggtgggctt tgccagaaaa cacgaattgt
      421 tcgacccggc cattatccct gggaccacca ccctctatca gtggatcgac cgtggcatca
      481 tgaaaacgaa gaatatggac ctgctagaaa aactgtcccg caaaccgaaa tcgtcccgtc
      541 atggcaaccg cccgaataaa cgggtgctag ggaaatccat tgaagagcgc ccggaggcgg
      601 ttggtacccg gaaaaccttc ggccattggg aagtcgacac ggtcgtagga aacaagacca
      661 agtcggatgc cgtgctgctg acgttggtgg aacggcagac acgcttcgag gtcattctga
      721 agctggccgg gaaggatgcc gaatcggtca accaagcggt ccgacagctg agagaacggg
      781 ctggtgcgta cttcggccag ctgttcaaga cggttacctc ggacaacggc tcggagttcg
      841 cgggactgca cgatgccttg caggatgtgg caaacatcta cttcagccac ccctatgctt
      901 cctgggagcg cgggacgagc gaaaaccaac acaagctgat tcgccgtttc atccccaagg
      961 gcagcccaat cggcaacgtg agcgaaaacc aaatcttgcg gattcagcgt tggatgaacg
     1021 attacccgcg aaagattttg ggctatgcaa cgccgcatga cacgttcgtc caggcattta
     1081 ggcaggaacg gttgacggct tgacacgtgg gcccttgaca acgagcctcc ctcggcatgg
     1141 cttgtgtctg gaaagctggc cggggccagc cagtcgggag actaccatgc ctcagctcat
     1201 tgtcaagggc ccactcgatt agaaaaatta gacggttggt ggtaacgagc aaccgggtca
     1261 ccatg
//
LOCUS       DACDXE010000201         1246 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.216, whole genome shotgun sequence.
ACCESSION   DACDXE010000201 DACDXE010000000
VERSION     DACDXE010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.216"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            200..742
                     /locus_tag="IWM23_003036"
     CDS             200..742
                     /locus_tag="IWM23_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885599.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IWM23_003037"
     CDS             832..>1246
                     /locus_tag="IWM23_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAP9885600.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACDXE010000202         1199 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.217, whole genome shotgun sequence.
ACCESSION   DACDXE010000202 DACDXE010000000
VERSION     DACDXE010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.217"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>1199
                     /locus_tag="IWM23_003038"
     CDS             <1..>1199
                     /locus_tag="IWM23_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAP9885601.1"
                     /translation="QKKKTLVVDAVLSPTPRACRSCGSTVVDGNGKAIIVKNGKKETI
                     VRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQSYFVQPRHSIANHVRYKIASLLTEKV
                     SLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSKKILPRVLMVDEFRSHASIEDKMSFIC
                     ADGETGKLIDVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQLTKRVLPNAKVV
                     IDRFHIVKHMNQAFNELRIREMNELRKAGQKSQAEKLKKNWRFLLKNRANINHYEYKT
                     WKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAYPFFHELVEAFRDKDPDLFFSLLAELP
                     ETLDDSFREKLQNLLTYEEGITNAMIYPYSNGKIEAKNTHIKTMKRVSYGFKSFENMR
                     IRIFLIN"
BASE COUNT          396 a          237 c          231 g          335 t
ORIGIN      
        1 ttcagaagaa aaagacgttg gtcgtcgatg ctgttctctc gcctactcct cgtgcttgta
       61 gaagttgtgg ttctactgtg gtagatggaa acgggaaagc aattatagtg aaaaatggaa
      121 aaaaggaaac gattgtccgt tttgaacaat acaatcatat gcctttggtt atgcgcctaa
      181 aaaagcaacg ctatacctgt aaaaactgtc gcacccattg gacggctcaa agttattttg
      241 tccaacccag acattcaatc gcaaatcatg ttagatataa aattgcttct ttactgactg
      301 aaaaagtatc tttatctttt attgcgaaaa gctgtcaggt atctttgacc accgttattc
      361 gtacattgaa agagtttaaa agctatttac caaagcaatc taagaagatc ctccccagag
      421 tattgatggt tgacgaattt cgttcgcatg cttccataga agataagatg agctttattt
      481 gcgcggatgg cgaaacagga aaattaatag atgttttgcc tacgcgtaaa ttacctcgat
      541 taacaagcta tttcttaggt tgtaccaatc cagaagaagt agaattcttg gtgacagaca
      601 tgaacgccgc ctacttccag ctcaccaaac gtgttctgcc aaatgcgaaa gtcgtgattg
      661 atcggtttca tattgtcaaa cacatgaatc aagcgttcaa tgagttgcgt atccgtgaaa
      721 tgaatgaact tcgtaaagcc ggacagaaaa gccaggcaga aaaactgaaa aagaactggc
      781 gctttttgct aaaaaatcgt gcaaacatca accattatga atacaaaaca tggaaaagtt
      841 tccgagcacc aaaataccca tttcttactg aagcaatgat gattgatcga ttgcttgagt
      901 tttctccgcc cctaaaggag gcgtatccct tttttcatga attagttgaa gcctttcgag
      961 acaaagaccc tgacttattt ttctccttat tggcagaact tcccgaaacg ttagatgaca
     1021 gctttcggga aaagcttcaa aaccttctga cctatgaaga aggcatcacc aacgcaatga
     1081 tctatcctta ttccaatgga aaaatagaag cgaagaatac ccacataaag acaatgaaac
     1141 gagtatccta cggatttaaa tcatttgaga acatgagaat tagaatcttt ttgatcaat
//
LOCUS       DACDXE010000203         1192 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.218, whole genome shotgun sequence.
ACCESSION   DACDXE010000203 DACDXE010000000
VERSION     DACDXE010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.218"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1076
                     /locus_tag="IWM23_003039"
     CDS             <1..1076
                     /locus_tag="IWM23_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9885602.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DACDXE010000204         1143 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.219, whole genome shotgun sequence.
ACCESSION   DACDXE010000204 DACDXE010000000
VERSION     DACDXE010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.219"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWM23_003040"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="IWM23_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAP9885603.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DACDXE010000205         1084 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.220, whole genome shotgun sequence.
ACCESSION   DACDXE010000205 DACDXE010000000
VERSION     DACDXE010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.220"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            283..816
                     /locus_tag="IWM23_003041"
     CDS             283..816
                     /locus_tag="IWM23_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAP9885604.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          349 a          202 c          201 g          332 t
ORIGIN      
        1 gagggtcagc tgattgaaca ccgtttagtg gagaagttgt gaaaaagtcg cctactttcg
       61 agtcgtaaga aatttttttg aaaatggttc ctcacaactt tgcataaatt tgacttaata
      121 ccgagaagtc atgatcccca tctaactcca aatcacaagg aatgagttca aaaactagct
      181 tttctcaaca aatattacat aaaaaattct atgttttttg ttaaatttat ttgctttttt
      241 caaaagtatg ttacagtaat ttcccaagga ggttttacag aaatgaattt tgttgccatg
      301 gattttgaaa ctgccaatca tcagccttat agtgcttgtt ctcttgcgct agtcatggta
      361 aaaaatagcc agatcgttga tgaattctat acattgatac agcctgagac tccttttttc
      421 tggcgaaatg tccaaattca cggaatccat caagaagacg tacgcaatgc gccgaaattc
      481 ccagaagtct ggcaaactat ccaaccatat tttcaaaaaa atcgtttagt cgttgcacat
      541 aatgctgctt ttgatacaaa ggtcttagcg ggatgcttag attattatca tcttgaacag
      601 cctaattatc tttctttatg tacagtgaaa agtagccgtc gcctgtttcc tgaatttcca
      661 aatcatcgat tgaacactgt gtgcgaaaat ttgaacatcc aattagatca tcaccatgat
      721 gcattggaag atagtcgtgc atgtgcggaa atcctgcttt atcaagaaaa aaaattcggg
      781 acagatccat tgaaaaaact tgtgactata aagtaaaaga atacttatga aacacacaaa
      841 gaggtcgtga gcctgccgtt tcactccgag tcacaaggag caatttcaaa aaatagcttc
      901 tcatgtttat taaatattgt gactggtgac cgaggagctg gcaggagaac accgtttatt
      961 ggagaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa caaaaatagc
     1021 ttctcatatt tttcatttgg ttggacttat taccgcagat gcaagctttt ggacaccgtt
     1081 tacc
//
LOCUS       DACDXE010000206         1079 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.221, whole genome shotgun sequence.
ACCESSION   DACDXE010000206 DACDXE010000000
VERSION     DACDXE010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1079)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.221"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            33..1079
                     /locus_tag="IWM23_003042"
                     /pseudo
     CDS             33..1079
                     /locus_tag="IWM23_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
BASE COUNT          452 a          144 c          191 g          292 t
ORIGIN      
        1 gactagaaat ctaattaaaa ggggtgcttt ttttgcggaa atatacattt gtttttaaga
       61 aaaaagtagt ttcagactat ttaaataacg aaggcggcta caaatatctt gcacataaat
      121 atcaaataaa tcgtacactg gttagacatt gggtaaggat ttataactat catggttggg
      181 aaggcttggt tggaggtggc aaaagctaca ctacaaaatt taaacttgat gttatagaat
      241 atatggaaac aaatggtctt tctatccaag aaactgctaa aaaatttaat atcggttcaa
      301 atagaactct aagtaaatgg atagagcaat atgaagaagg cggtgcttct tcacttgaga
      361 gccaaaaaag gggcagaaaa attagtatga attccaagct aaacattcct aaaaaactta
      421 aagatgagtc tcttgaagaa gaagttattc gtttaagagc agagaacgca tatttaaaaa
      481 agttagaaac cttgattcaa gaacaagatt tgagcaagaa gaaatcaagg ttaaaacaat
      541 ccttcagctc aaaaaagaat ttaaattgaa tctattactt tcaattgcac aattagcgaa
      601 gtcaacttat tattactggg taaaaaaatt agataagcca gataaatata gcaagataaa
      661 gcaagaaatt acagcgattg taaaagaatc aagaaactct tatggttatc gtagagtcac
      721 tttagcgtta aagatgaaag gatatacaat caatcataaa acagttagaa aattaatgtc
      781 ccaaatggga cttacctgtc aaatccgaat aaagcgatat aaatcttata aaggaacagt
      841 aggaaaaatt gccaagaatg tgttaaaacg aaatttttca gtagatacac ccaataaaaa
      901 atgggtgaca gacgtcactg aattcaagat aaaaggaaga aaaatctatc tatctcctat
      961 acttgattta tttaacggag aaataattag ttatagtata tcaactagtc caacatataa
     1021 actgattgaa gaaatgcttc aacaagcaat taaaaaaaag ggaactgagg gttcactga
//
LOCUS       DACDXE010000207         1062 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.222, whole genome shotgun sequence.
ACCESSION   DACDXE010000207 DACDXE010000000
VERSION     DACDXE010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.222"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(37..993)
                     /locus_tag="IWM23_003043"
     CDS             complement(37..993)
                     /locus_tag="IWM23_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885605.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DACDXE010000208         1049 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.223, whole genome shotgun sequence.
ACCESSION   DACDXE010000208 DACDXE010000000
VERSION     DACDXE010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.223"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..978
                     /locus_tag="IWM23_003044"
     CDS             4..978
                     /locus_tag="IWM23_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAP9885606.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          375 a          132 c          182 g          360 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacattttt
//
LOCUS       DACDXE010000209         1035 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.224, whole genome shotgun sequence.
ACCESSION   DACDXE010000209 DACDXE010000000
VERSION     DACDXE010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.224"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            25..249
                     /locus_tag="IWM23_003045"
     CDS             25..249
                     /locus_tag="IWM23_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAP9885607.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            269..556
                     /locus_tag="IWM23_003046"
     CDS             269..556
                     /locus_tag="IWM23_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9885608.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
BASE COUNT          372 a          123 c          227 g          313 t
ORIGIN      
        1 aaaaaacgaa aaggaaaaac aatcatgaaa aagaaagtat taaaacattg tgttattcta
       61 ggaatattag gaacttgtct agctggcatc ggtacaggaa taaaagttga tgcagctact
      121 tactatggaa atggtcttta ttgtaacaaa gaaaaatgtt gggtagattg gaatcaagct
      181 aaaggagaaa ttggaaaaat tattgttaat ggttgggtta atcatggtcc atgggcacct
      241 agaaggtagt ggtagggagg ttattataat ggattttact aaagaagaaa aacttttaaa
      301 tgcaattagt aaagtataca atgaagcaac tatagatgac tatcctgact taaaagaaaa
      361 gctctttctt tattctaaag aaatcagtga gggaaaaagt gttggtgaag ttagtatgaa
      421 attaagtagt tttcttggaa gatatatttt aaaacataaa tttggattac ctaaatcttt
      481 aatagaatta caagaaattg ttagtaagga atctcaagta tatagaggat gggcttctat
      541 tggtatttgg agttaattct ttggaataaa atgaaaatgg gactgaagaa tcaattctga
      601 ttcatcagtc ccataaatta tagagccgaa aaattatcat atttatagat ttattaatag
      661 taaatggcat aagaatcttt gaacaacgaa aaaaatgtag ctctacctaa ttattgattt
      721 gcaacaattt caccgaaggt gaaacaagcg aaactttaca aaaagtggct agcatttgct
      781 agtctttttt gtattgtgga gagtgggggg gagggggatt caagggggag cttgcgcccc
      841 tttgacaagc ggttatttgc accaaatacg taggtatttg gctagcaaat atagagcttg
      901 ccaagccctt tagtgagctt tagtgagctt tacttattgt acctttttag gtacaatggt
      961 attgcgaacg atacgtccgt aggacgtact gaaagggtgg gacttatgag cgaacaaaat
     1021 caatatttgg ctagc
//
LOCUS       DACDXE010000210          996 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.225, whole genome shotgun sequence.
ACCESSION   DACDXE010000210 DACDXE010000000
VERSION     DACDXE010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.225"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(25..912)
                     /locus_tag="IWM23_003047"
     CDS             complement(25..912)
                     /locus_tag="IWM23_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAP9885609.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          322 t
ORIGIN      
        1 aatttctttg cctttttatc taaatcaaac gtttagttct tgaccaacta ataaagaata
       61 actggacata gacatgccat ttttcgccat tagatcatca acagacatgc caaatttttg
      121 tgcaatatcc catagcgtat cacctgacac gactgtatat gtctgaccat tatttgtatt
      181 tacatctgtt gttgaagatg atgacgttga agtcgtcgta gtagtcgttg ttgttgtact
      241 tgtggttgtt gtagccgaac ctgttgatgg cgtatcaaaa cgggttaaat tataagtatt
      301 aatcaatgag ttcaatttgg ccccataatt tggatcggta gcataacgac ctgtcaagta
      361 cgctgttgca tcataaaaac ttgttgtatt gcttttccac acgccagcat aatgataatc
      421 gcccgtggaa aaactggttg aacgaagaac attcgcatgg tcttggaagg attcccaata
      481 agaagaatat tttcggaatg gctcattcat cgttacccat tgtccgttta catattcttt
      541 ggttgccata taaacgacct gtccgttata gcttcctttc acaccgaata gattataatt
      601 tggcgcacta gctaatccgg aagtgccata tgcactttcc aacaacgctt gagcgatcat
      661 gactgaagca tacaagtcat tacttgcagc aacttctgac gcagaccagc cgatttgatc
      721 gataaacgct tgaggatcag cgactgtttg agatccttgt tcatctgcat gaacatgttg
      781 cggtaccgct gtaaccatca tcggcccaac taaaagactc gttccaatta aaggagcaat
      841 cgctttttta ttgatcacca tttttctttt aggtctatgg tgccgggcac tgcgggtctt
      901 cagctcttgc attcaaattc ctcctcaaaa taaaaaaact taataaaaag ttctcttctt
      961 aaaacaaact taattaatta aattatactt ttaatt
//
LOCUS       DACDXE010000211          991 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.226, whole genome shotgun sequence.
ACCESSION   DACDXE010000211 DACDXE010000000
VERSION     DACDXE010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.226"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(21..94)
                     /locus_tag="IWM23_003048"
     tRNA            complement(21..94)
                     /locus_tag="IWM23_003048"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="IWM23_003049"
     tRNA            complement(108..181)
                     /locus_tag="IWM23_003049"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="IWM23_003050"
     tRNA            complement(209..281)
                     /locus_tag="IWM23_003050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="IWM23_003051"
     tRNA            complement(301..374)
                     /locus_tag="IWM23_003051"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
     gene            complement(384..457)
                     /locus_tag="IWM23_003052"
     tRNA            complement(384..457)
                     /locus_tag="IWM23_003052"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Arg,seq:acg)
     gene            complement(468..553)
                     /locus_tag="IWM23_003053"
     tRNA            complement(468..553)
                     /locus_tag="IWM23_003053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(517..519),aa:Leu,seq:taa)
     gene            complement(570..641)
                     /locus_tag="IWM23_003054"
     tRNA            complement(570..641)
                     /locus_tag="IWM23_003054"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(607..609),aa:Gly,seq:gcc)
     gene            complement(653..725)
                     /locus_tag="IWM23_003055"
     tRNA            complement(653..725)
                     /locus_tag="IWM23_003055"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(690..692),aa:Thr,seq:tgt)
     gene            complement(743..824)
                     /locus_tag="IWM23_003056"
     tRNA            complement(743..824)
                     /locus_tag="IWM23_003056"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Leu,seq:tag)
     gene            complement(832..904)
                     /locus_tag="IWM23_003057"
     tRNA            complement(832..904)
                     /locus_tag="IWM23_003057"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Lys,seq:ttt)
     gene            complement(908..980)
                     /locus_tag="IWM23_003058"
     tRNA            complement(908..980)
                     /locus_tag="IWM23_003058"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Val,seq:tac)
BASE COUNT          260 a          283 c          235 g          213 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat taacgcgccc aagaggagtc gaacccctaa ccttttgatc
      421 cgtagtcaaa cactctatcc agttgagcta tgggcgcata tttgaaatgc cgaggaccgg
      481 aatcgaaccg gtacggtgat cactcaccgc aggattttaa gtcctgtgcg tctgccagtt
      541 ccgccacccc ggcgcgattg ctttggcaaa gcggaaaacg gggttcgaac ccgcgacccc
      601 caccttggca aggtggtgct ctaccactga gctatttccg cgtgtttaaa gatgccggct
      661 aaaggacttg aaccctcgac cctctgatta caaatcagat gctctaccaa ctgagctaag
      721 ccggcatgta aaacttaaat tatgcgggtg aagggacttg aacccccacg ccgtaaggcg
      781 ctagatccta aatctagtgc gtctgccaat tccgccacac ccgcaaaaat atgagccgta
      841 cagggctcga acctgtgacc ctctgattaa aagtcagatg ctctaccaac tgagctaacg
      901 gctcatatgg aggttaacgg gatcgaaccg ctgaccctct gcttgtaagg cagatgctct
      961 cccagctgag ctaaacctcc atggataact t
//
LOCUS       DACDXE010000212          984 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.227, whole genome shotgun sequence.
ACCESSION   DACDXE010000212 DACDXE010000000
VERSION     DACDXE010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..984
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.227"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..704
                     /locus_tag="IWM23_003059"
     CDS             <1..704
                     /locus_tag="IWM23_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885610.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          332 a          161 c          163 g          328 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aata
//
LOCUS       DACDXE010000213          966 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.228, whole genome shotgun sequence.
ACCESSION   DACDXE010000213 DACDXE010000000
VERSION     DACDXE010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..966
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.228"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..461)
                     /locus_tag="IWM23_003060"
     CDS             complement(<1..461)
                     /locus_tag="IWM23_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAP9885611.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
     gene            complement(745..951)
                     /locus_tag="IWM23_003061"
     CDS             complement(745..951)
                     /locus_tag="IWM23_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAP9885612.1"
                     /translation="MNLIYDEIAIDPYKGEQKTGDLSGIWSSGFKYAGTTYRVAYEII
                     ENKVIPVLLCGTHENFYEQLKKLI"
BASE COUNT          312 a          184 c          128 g          342 t
ORIGIN      
        1 ttactatata cagaaataga aggtgggaca tctaaataga tgacgtcaaa ctcatcaaat
       61 aatggttcta gcttttcttt catatatttg aattgggata atgaatcatt atattggtct
      121 ttgatccaat catcatacat ttcaaaggac atgtctgctg gaagtatata gaggttattg
      181 attactttat agacagcctc tttaggtgga agattgttta tcaacacgtc agtcacagta
      241 ttttcgtaaa caatcggttc accttctccg cctccttctt cgttagtgaa gttcatatgc
      301 gttaagttca tcacatcaga agtatttcct tgcatatcca agtcaattat caaggttctt
      361 tttccttcat taactgctga ttcaaaccct aacatttgcg cgcacgtact tttacctacg
      421 ccacctttaa aattaccgat tacatatttc ttcgctacca cgttcctacc tcttttcttt
      481 tagcaatatt catttgatac tattataaca tatttatttt tagttactac caaaaaaaga
      541 tatatatgta ctaccttata attaataagt aaaaggtagt acataaataa ggaaaagctg
      601 aaatatattg atttatagct ttcctcaaag taatgacgtt atttaattta tcgagaagca
      661 gagaatactc gcgactttag tcatgtagac gaatcagatt ttcgataaaa agaagccgtc
      721 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
      781 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
      841 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
      901 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
      961 tttcaa
//
LOCUS       DACDXE010000214          875 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.229, whole genome shotgun sequence.
ACCESSION   DACDXE010000214 DACDXE010000000
VERSION     DACDXE010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.229"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..101)
                     /locus_tag="IWM23_003062"
     CDS             complement(<1..101)
                     /locus_tag="IWM23_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9885613.1"
                     /translation="MQELVILKNKEAVTTSLQVADSFEKKHKHVLEA"
     gene            155..616
                     /locus_tag="IWM23_003063"
     CDS             155..616
                     /locus_tag="IWM23_003063"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885614.1"
                     /translation="MKKLLYILLFSTFAIVALLLLFEHLKRVNLDGTLLEEQIAILNT
                     PKLLIVFFFMCMLSALIFMFYISTFWVYKEIKNKEFTDKDMIFMGFLVKILFIFSIVI
                     TLLFCLNETQFSVATTLISFIAIFSFIFSDNQKKSINGFLQKIKKKINGEP"
BASE COUNT          269 a          147 c          128 g          331 t
ORIGIN      
        1 attgcttcta gcacatgctt gtgtttcttt tcaaaactgt ctgcgacttg taagctcgta
       61 gttacagctt ctttattttt caaaattact aattcttgca ttttttcttc tctccttttg
      121 atataattta ggtaaaaaat ggtggtgaaa atatttgaaa aaattgctat atattttact
      181 tttttcaacc tttgcaattg ttgcattatt gttgcttttc gaacatctta aaagagtaaa
      241 cttggatgga actttgttag aagagcaaat tgcgattcta aatactccca aattactaat
      301 tgtattcttt tttatgtgta tgttatccgc tctgatattt atgttctata tatcaacttt
      361 ttgggtatac aaggagatta aaaataaaga gtttactgat aaagatatga ttttcatggg
      421 ttttttagtc aaaattcttt ttatattttc catagtaatc actttattat tttgtttaaa
      481 tgaaacacaa ttttctgtag cgaccacttt gatttcattt attgctattt tttcctttat
      541 attttccgat aatcagaaaa aaagtattaa cggatttctg caaaaaataa agaaaaaaat
      601 caatggagaa ccctaatata aaaatcagga aaaaaatatt gattactcct cttaggaaga
      661 ccattagtca gtccctcccg gctggctttt tcgctctgta ctcagcttca tcaagcccca
      721 taaaaatcca gaccatatat acgattgtgc cgattaatgc ttgtctgctt ccccaaagac
      781 ctaaagcgta gatgatgaat ggtgtgctga atactagcgc tctgttgaat ttgcccatta
      841 gcttgcctcc ttcaaaacat ctgaaggcga taaac
//
LOCUS       DACDXE010000215          855 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.230, whole genome shotgun sequence.
ACCESSION   DACDXE010000215 DACDXE010000000
VERSION     DACDXE010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.230"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..101)
                     /locus_tag="IWM23_003064"
     CDS             complement(<1..101)
                     /locus_tag="IWM23_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAP9885615.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEA"
     gene            154..630
                     /locus_tag="IWM23_003065"
     CDS             154..630
                     /locus_tag="IWM23_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885616.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            complement(636..818)
                     /locus_tag="IWM23_003066"
     CDS             complement(636..818)
                     /locus_tag="IWM23_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885617.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
BASE COUNT          260 a          163 c          122 g          310 t
ORIGIN      
        1 attgcttcta gtacatgctt atgtttcttt ttgaaacttt ctgccacttg taagctcgta
       61 gttacagctt ctttattttt caaaattact aattcttgca ttatttcttc tctccttttt
      121 ggtataattt tcgttagaaa gtgaggtgat tctatgagga aaaagctcaa ttacattctt
      181 actgcgactt taaccatata tataattctg tttattacct tatctttcct gtcttcaatt
      241 aaaaaaattc cttttattac attgaataaa aatagtattt tgaaggagtt caataacact
      301 gactacatcg catttctaaa acttttttca gtctgtgtgc taatatcatg tgtaattatt
      361 tttattgttg ctaaaacatg ttggacaatg ttaaataaaa accaacgcga tgttgaaaat
      421 attgctatat ttttaattaa gattacttcc tcaatatcaa ttatcattcc gatgactgta
      481 actttttcaa aagaacagtt taatgtagcg gctacttatc tttcatttat agcgctattt
      541 tcttttgttc ttcctaaaag aaataagagc aactttgaca acaaggagct ggatgaagta
      601 cgcaatagca gcccaaagga acaaaaataa aaaaatcata ttttagtcag tccctcccga
      661 ctggcttttt tcgctctgtg ctcagcttca tcaagcccca taaaaatcca aaccatgtaa
      721 acgattgtcc ctatcaacgc ttgtctgctt ccccagaggc ctaaagcgta gatgattagc
      781 ggtgcgctga atactagtgc tctgttgaat ttgcccatta acttgcctcc ttcaaaacat
      841 ctgaaggcga taaac
//
LOCUS       DACDXE010000216          845 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.231, whole genome shotgun sequence.
ACCESSION   DACDXE010000216 DACDXE010000000
VERSION     DACDXE010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..845
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.231"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..304
                     /locus_tag="IWM23_003067"
     CDS             <1..304
                     /locus_tag="IWM23_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAP9885618.1"
                     /translation="ITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGIL
                     RRNGLPKSMDFREVNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            627..743
                     /locus_tag="IWM23_003068"
     CDS             627..743
                     /locus_tag="IWM23_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HAP9885619.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          335 a          106 c          122 g          282 t
ORIGIN      
        1 tattacgttt gactgtggaa aagaattttc taactggaaa gccattagta accaacatga
       61 tattgatata tattttgcgg accctggaac accttctcaa cgcccattaa acgagaattc
      121 taacgggatt ctgcgtcgta atggactgcc gaaatcaatg gattttagag aagtgaatca
      181 gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat cattgaatta
      241 cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt ctagcttaat
      301 ttgacaaatc ataaataata aagaattgaa cttctgagaa aacaaagaat cttttttctt
      361 tttttgaatt gactcatgga ttggcgttaa aattttattt tgaaaaagtt atttctggtt
      421 taactcaaaa aaagagatat gaatctaata gtgatagaaa ggatgttatt tttcatgaaa
      481 tggttgtttt agatttaata catataacta atgaatgcta ttgaatgtat ttaaactaat
      541 taacaaaaga atttgaattg ttaataaaat ccaataaaat atattaataa agtagataga
      601 aataaaaaaa caaagactaa acatatataa ttggattaaa cgcaatatct gcaaaatatt
      661 gtaaatttaa agcctttgta gaaaaattta ctgaaagaca aactgataag attattcagt
      721 atcatattac ctatactgat taaaattttt tgaaacggga tactaaataa aaaatttatt
      781 tataacagaa ctaattattt aatgcggatg cgccaatact tggtcctgca agagctattt
      841 atttt
//
LOCUS       DACDXE010000217          843 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.232, whole genome shotgun sequence.
ACCESSION   DACDXE010000217 DACDXE010000000
VERSION     DACDXE010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.232"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..780)
                     /locus_tag="IWM23_003069"
     CDS             complement(<1..780)
                     /locus_tag="IWM23_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAP9885620.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTK"
     gene            complement(796..>843)
                     /locus_tag="IWM23_003070"
     CDS             complement(796..>843)
                     /locus_tag="IWM23_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011058339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885621.1"
                     /translation="DKSIYHWDFYALLGF"
BASE COUNT          248 a          170 c          123 g          302 t
ORIGIN      
        1 ttttgtatat tcaattttga aaacatttcc gcaaagttca gacggacctc gatgtgttga
       61 tgaataaaat ttattagtaa taacttctat aaggttatca atccctatat tactttttgc
      121 acttccatga taaagaggga acagagaaca attctgaaat cttatgcttt cctcttgttc
      181 gagttccaat gcttctaatg atttaccgga catatatttc tctaaaaggt catcgtttcc
      241 ctctattacc gtatcccatt gttcagattc ggtaaagttc gtcacacaca tattaggata
      301 cagttctacc ttctgtttga ttacaatttc ggcagaaagt ttctctttaa tatcctgata
      361 aaccgttgat aaatcaattc cattttggtc aatcttattg ataaaaaaga ttgtgggaat
      421 ccccattttc ctaagtgcat gaaataatat acgagtttgt gcttgtacgc catcttttgc
      481 agaaatcagt agaattgccc catctaaaac tgataatgaa cgatatactt ctgctaagaa
      541 atccatatgt cctggcgtgt ctatgatgtt caccttcgta ttttcccact gaaaagaggt
      601 tattcctgtc tgaattgtaa ttcctctctg acgttctaaa agcgtattat ccgtcctcgt
      661 tgtacctttg tccacgcttc ctaattctgt aatcgctcca ctgttatata ataagctttc
      721 tgttaaggta gtttttcctg catcaacatg agctaaaact ccaatattaa taattttcat
      781 gtgattttcc tccattcaaa aacccaaaag ggcataaaaa tcccagtgat aaatactttt
      841 atc
//
LOCUS       DACDXE010000218          799 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.233, whole genome shotgun sequence.
ACCESSION   DACDXE010000218 DACDXE010000000
VERSION     DACDXE010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.233"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..395
                     /locus_tag="IWM23_003071"
     CDS             <1..395
                     /locus_tag="IWM23_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885622.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..>799
                     /locus_tag="IWM23_003072"
     CDS             398..>799
                     /locus_tag="IWM23_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9885623.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPS"
BASE COUNT          319 a          133 c          148 g          199 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcg
//
LOCUS       DACDXE010000219          782 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.234, whole genome shotgun sequence.
ACCESSION   DACDXE010000219 DACDXE010000000
VERSION     DACDXE010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.234"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            159..500
                     /locus_tag="IWM23_003073"
     CDS             159..500
                     /locus_tag="IWM23_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885624.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            493..>782
                     /locus_tag="IWM23_003074"
     CDS             493..>782
                     /locus_tag="IWM23_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9885625.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          300 a          120 c          155 g          207 t
ORIGIN      
        1 aatagggacg gtaattgcta atcgctggtg gagacacaat aaaccaaaca tcctaagcca
       61 acaacatatc gaaaagcaaa aagcatggca acaaataaaa aagtgactca gccggcaagc
      121 atagagtcac aaaacatatc taaggagaat tttagcatat gaataaagaa ctttccactt
      181 tagatcaata tttgactgat tctgaatggg gcaagtcgaa tatcaaggaa acaaataatc
      241 gaaaaatcag acgaaatctt ttgacgaacg aagaactatc atgtgatcaa gatgacttgg
      301 gaaattttgt gagtatttgg gatcatgttt accttatcca tctatcaaaa cattcaaata
      361 aacctgaata catctatgtc atcgaagatg gcttgattga tgcgctagaa gagtacaaca
      421 gagataactt gattgatatc tcttattacg gatcaggtaa gaaatacatt gctgaaatgg
      481 aggcagaatt tgatgagtga aatcaaaggg acaacgaact ttgaaaaact ttttagtcgt
      541 aagttaaata aaattctcaa gaaaaaagga aattttgatt atttatcttg ggctcacgcg
      601 tgggagatta tgaaaaagaa tgatccacag gcaacggtaa ctattaatga gtataaacac
      661 tacagggttg tttctggaac tcatcaagac tttcttgttg aggaatataa accttttctt
      721 atggatgaaa cagggactta tgtatctgtc tcagtaacgg ttaaaggaca tacggaaaca
      781 ga
//
LOCUS       DACDXE010000220          776 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.235, whole genome shotgun sequence.
ACCESSION   DACDXE010000220 DACDXE010000000
VERSION     DACDXE010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.235"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..505
                     /locus_tag="IWM23_003075"
     CDS             <1..505
                     /locus_tag="IWM23_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885626.1"
                     /translation="RNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLKDES
                     NTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKTGAD
                     IDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQETI
                     KKLRIGG"
     gene            509..>776
                     /locus_tag="IWM23_003076"
     CDS             509..>776
                     /locus_tag="IWM23_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAP9885627.1"
                     /translation="MDHRSYYAIIPANVRYDDSLIPSAKLLYGEITALCNEKGYCWAS
                     NEYFANQYGVRKSTIQNWLKSLEEKGYIYREVKYKEGSREIEARY"
BASE COUNT          278 a          135 c          164 g          199 t
ORIGIN      
        1 acgaaacttc atctatgcgc tgataggcga tatttttatc gatacaggca tgccaacgga
       61 cttctggaag gaattcttct acttccgttt tgaaggcgtc acagggcgca aaataagcct
      121 caaagacgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata tcattttaga
      181 tttcatcttt gaacaccata ttcctttcaa agaaggctat gagattttac cagcgaatca
      241 agagtattac ttctacaaat gcatcacaaa aagagtctgc tgcatctgtg gcaaaacagg
      301 agctgatatt gatcactttg acaaagcttt aggaagacga aagcgcaaag aagttgatca
      361 ttcagagtac acatttgcag cactctgcag aatccatcac acagagaagc acaagatagg
      421 tgtgatcaat ttcaaaaata agtatcaaat caaagggatc aagttaaacc aggaaacaat
      481 caagaaatta agaatcggag gatgaaaagt ggaccacaga agttattacg ctatcatacc
      541 tgcaaatgtt aggtatgacg attctttgat acctagtgca aaacttcttt atggagaaat
      601 cacagctcta tgtaatgaga aaggttattg ctgggctagc aatgagtact ttgccaatca
      661 gtacggtgta aggaaatcaa cgattcaaaa ttggctgaaa tctcttgaag aaaaaggcta
      721 tatctatcga gaagtgaagt acaaagaggg tagtagagaa atcgaggcta ggtata
//
LOCUS       DACDXE010000221          733 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.236, whole genome shotgun sequence.
ACCESSION   DACDXE010000221 DACDXE010000000
VERSION     DACDXE010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.236"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            2..>733
                     /locus_tag="IWM23_003077"
     rRNA            2..>733
                     /locus_tag="IWM23_003077"
                     /product="23S ribosomal RNA"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DACDXE010000222          729 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.237, whole genome shotgun sequence.
ACCESSION   DACDXE010000222 DACDXE010000000
VERSION     DACDXE010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.237"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            450..668
                     /locus_tag="IWM23_003078"
                     /pseudo
     CDS             450..668
                     /locus_tag="IWM23_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          247 a          113 c          118 g          251 t
ORIGIN      
        1 ttttttacgt ttctatatta tagaattaac gggtaggctt actgtctttt ttggcaattt
       61 tgcaatcaag ccatttgcag aggtaactgc acactacatc tgcaagtggc ttgattgcaa
      121 aaacaaaatc aaaaaggtat ttcattatat ctcctcctcc ccttgccaga ttaggccggg
      181 gtagcagaca gcaaaactat tataacataa ctgaaatacc taaatgacat atacagatat
      241 gataatttat gtattgattt ttctaacaaa agcacatatt ttaatatata ctacctattt
      301 taggtagtat atatttttag atacaatatc gatcagtttt gaggaaatga aattctagat
      361 attttttgct acatagagtt actttcaata ttttttaacg taattaggca gaagtcactc
      421 atcctctata ggtttgtgat gaaattgcca ttgatccatt taagtaagag caaaaaactg
      481 gaggtttatc aggtaattgg tctaatggat ttaagtatgc ggtcaccgtg tatagagttg
      541 cttatgagat tcaagaaaat aaagttattc cagtattgct atatggaaca cataacaatt
      601 tttatgaatt agccgagaac actcgcaact ttattcgtgt agatgaatcg gcttcggttg
      661 agttataact ttagctatat caatcaaccg accgacatat gttaaatcta tatataataa
      721 aaataccta
//
LOCUS       DACDXE010000223          716 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.238, whole genome shotgun sequence.
ACCESSION   DACDXE010000223 DACDXE010000000
VERSION     DACDXE010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.238"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            259..540
                     /locus_tag="IWM23_003079"
                     /pseudo
     CDS             259..540
                     /locus_tag="IWM23_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            614..>716
                     /locus_tag="IWM23_003080"
     CDS             614..>716
                     /locus_tag="IWM23_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HAP9885628.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQEL"
BASE COUNT          257 a           91 c          125 g          243 t
ORIGIN      
        1 aagtttttga tcctttattt tcaagtctga tttggcagct tttcggcagc tcatttattt
       61 atggtcagaa atagatgctg aaattttaat ctattagcaa atagtccaga acgtatgaag
      121 aggagtcgga tatcatttgt cattaacacc ataaatattt ataaaattcg tgttcaccta
      181 caattaaatt taatatataa tattcgttgt aagggcttat aattttttgt taatgaacaa
      241 gataggaaga aacatttaat ggacagtttt cagagattgt cactagtaaa tcaatttgag
      301 ttattggcta aatttgagga ccaaaacaga tcttattatg aaagaaaaat cgaaatattg
      361 agagaaggtt acgagtatca ttatgatgat gaaatttgga gtgatctttc tgagccattt
      421 ccgaaagaag attctcgatt tgtattagat gtattgaaca tgtatcgaga tatcaatttt
      481 agtaaatcaa agctaaactt agatgaaaac caaattacta attcatacta tactcatttc
      541 ggttctgttg caaagttttc caaaaaatct attttagtgt aaaattgaga aaaaagacag
      601 agaggacaga gtaatgaatc attttaaagg caaacaattc aaaaaagacg tcattattgt
      661 cgctgttggt tactacctgc gttacaatct aagctatcgt gaagttcagg aattgt
//
LOCUS       DACDXE010000224          712 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.239, whole genome shotgun sequence.
ACCESSION   DACDXE010000224 DACDXE010000000
VERSION     DACDXE010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.239"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..446)
                     /locus_tag="IWM23_003081"
     CDS             complement(<1..446)
                     /locus_tag="IWM23_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAP9885629.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DACDXE010000225          702 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.240, whole genome shotgun sequence.
ACCESSION   DACDXE010000225 DACDXE010000000
VERSION     DACDXE010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.240"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(91..>702)
                     /locus_tag="IWM23_003082"
     CDS             complement(91..>702)
                     /locus_tag="IWM23_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAP9885630.1"
                     /translation="IAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTYVRVGK
                     KWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGKEFDNQ
                     TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLELFDYV
                     HWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          194 a          164 c          130 g          214 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg at
//
LOCUS       DACDXE010000226          695 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.241, whole genome shotgun sequence.
ACCESSION   DACDXE010000226 DACDXE010000000
VERSION     DACDXE010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.241"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(53..628)
                     /locus_tag="IWM23_003083"
     CDS             complement(53..628)
                     /locus_tag="IWM23_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAP9885631.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          124 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaac
//
LOCUS       DACDXE010000227          688 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.242, whole genome shotgun sequence.
ACCESSION   DACDXE010000227 DACDXE010000000
VERSION     DACDXE010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.242"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            124..327
                     /locus_tag="IWM23_003084"
     CDS             124..327
                     /locus_tag="IWM23_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HAP9885632.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            327..677
                     /locus_tag="IWM23_003085"
     CDS             327..677
                     /locus_tag="IWM23_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAP9885633.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          287 a           69 c          115 g          217 t
ORIGIN      
        1 tcaatcattg ttatataata aaatgttata taataaaatt actaatgata tttaatatta
       61 caagcaatct taagaaattc gaaaaggaaa aaatgattat gaaaaagaaa gaaaaagaaa
      121 gtattgaaac attgtgttat tctaggaata ttaggaactt gtctagctgg cattggtaca
      181 ggaatagacg ttgacgcagc tacttactat ggaaatggtc tttattgtaa caaagaaaaa
      241 tgttgggtaa attggggaca atcatggtca gaagggttaa aacgttgggg agataatctt
      301 tttggtagtt ttagtggagg acgataatga cttcaataaa cattgatcac acaaaaaaag
      361 tcatacatga attatacaat agtttaatga cacgacctaa tcagacgaaa gcattattag
      421 atattactga tgttttattt caggtatata aaaaaattga tacagcaaaa tatcctgaaa
      481 ttcttgtaaa taaattagtt aattatattt acatcgtggg ttttgataat aaaatacgct
      541 ttattgataa tgatgaaaaa ttattaatag aattagggga tatttctaaa aaagctggaa
      601 ttaatagtaa atacaaagca aactttacag ataaatcgca attttatagt tattcagaaa
      661 caatgcctca acgataaaat atagtata
//
LOCUS       DACDXE010000228          616 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.243, whole genome shotgun sequence.
ACCESSION   DACDXE010000228 DACDXE010000000
VERSION     DACDXE010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.243"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            51..>616
                     /locus_tag="IWM23_003086"
     CDS             51..>616
                     /locus_tag="IWM23_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HAP9885634.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTT"
BASE COUNT          223 a          103 c          120 g          170 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccac
//
LOCUS       DACDXE010000229          581 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.244, whole genome shotgun sequence.
ACCESSION   DACDXE010000229 DACDXE010000000
VERSION     DACDXE010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.244"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            67..>581
                     /locus_tag="IWM23_003087"
     CDS             67..>581
                     /locus_tag="IWM23_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885635.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVS"
BASE COUNT          201 a           91 c          121 g          168 t
ORIGIN      
        1 agtggagtac tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt
       61 acagaaatgt atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga
      121 gaaattgtta ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa
      181 ctattgccac agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa
      241 ccgagtaaac ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt
      301 gatccgcttc tacgatatta cgtggattct acgacacatg aaattatact ttctttctta
      361 gggaaagtac aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata
      421 gaactaaaag agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc
      481 attcacatcg aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg
      541 cttccgttgg gaagtggaat gcagtatgag agctcggttt c
//
LOCUS       DACDXE010000230          538 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.245, whole genome shotgun sequence.
ACCESSION   DACDXE010000230 DACDXE010000000
VERSION     DACDXE010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.245"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IWM23_003088"
     CDS             complement(18..371)
                     /gene="tnpB"
                     /locus_tag="IWM23_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAP9885636.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          155 a          120 c           92 g          171 t
ORIGIN      
        1 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
       61 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      121 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      181 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      241 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      301 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      361 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      421 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      481 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DACDXE010000231          523 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.246, whole genome shotgun sequence.
ACCESSION   DACDXE010000231 DACDXE010000000
VERSION     DACDXE010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.246"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            27..>523
                     /locus_tag="IWM23_003089"
     CDS             27..>523
                     /locus_tag="IWM23_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAP9885637.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDAT"
BASE COUNT          191 a           96 c           86 g          150 t
ORIGIN      
        1 caattcacag aaaagaggac ttccctatga atgattttac tacagaaatt gtgcaaactc
       61 tagtcactaa aggcgattta aatgaattat tccgttcgca cttagaaaaa gcgataaaca
      121 cactcctacg gactgaatta acggcttttt tagattacga aaaatatgat cgcactggtt
      181 ttaattcagg taattcgaga aacggttctt actttcgatc aatcaaaacc gaatatggtg
      241 aattaacatt ggaaatacct agagatcgta atggtgagtt taaacaacaa actttaccag
      301 cctacaaaag aacaaacgat acattggaaa ccactattat ccatttattc gaaaaaggtg
      361 ttacgatgtc tgaaattgct gatttgatcg aaaaaatgta cggtcatcac tatactccac
      421 aaaccatgtc caacatgact aaagttctga ctgaagaagt aaatgccttt aaatccagag
      481 ccttaaatga taagtatgtc gctattttta tggacgctac tta
//
LOCUS       DACDXE010000232          521 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.247, whole genome shotgun sequence.
ACCESSION   DACDXE010000232 DACDXE010000000
VERSION     DACDXE010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.247"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>521
                     /locus_tag="IWM23_003090"
     CDS             <1..>521
                     /locus_tag="IWM23_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK signal domain/LPXTG anchor domain surface
                     protein"
                     /protein_id="HAP9885638.1"
                     /translation="ENPGENIPAGYHKVTFTAGEGTSIESGTTVFAVKDGVSLPEDKL
                     PVLKAKDGYTDAKWPEEATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNK
                     EPDAFEGIKNKEDLPKDTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTD
                     NRSDADKYEPTVEG"
BASE COUNT          211 a           84 c          120 g          106 t
ORIGIN      
        1 gaaaatccag gagaaaacat tcctgcaggc taccacaaag taaccttcac cgcaggagaa
       61 ggaacaagta ttgaaagtgg aacaacagtc tttgcagtga aagacggcgt aagcttacca
      121 gaagataaac ttccagtgtt gaaagcaaaa gatggttata cagatgcgaa atggccagag
      181 gaagcaactc aaccaattac agcagatgat acagaatttg tatcaagtgc aacaaaactg
      241 gatgataaat ctgacgctga caaatataat cctgaaggtc aaaaagtgac tacagaattg
      301 aataaggaac cagacgcatt tgagggaatt aagaacaaag aagatttacc aaaagatact
      361 aagtatactt ggaaagaaaa agtagatgtt agcgcagctg gaaataaaaa agggactgtt
      421 gtagtgacat atccagatgg atcatctgat gaagttgaag tagatgttac agtgacagac
      481 aatcgttctg acgccgataa atatgagcca acagtagaag g
//
LOCUS       DACDXE010000233          520 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.248, whole genome shotgun sequence.
ACCESSION   DACDXE010000233 DACDXE010000000
VERSION     DACDXE010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.248"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..512
                     /locus_tag="IWM23_003091"
     CDS             <1..512
                     /locus_tag="IWM23_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HAP9885639.1"
                     /translation="TEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTPEQPKPVT
                     PAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTGRDIGGWT
                     VPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESVKVGSVQW
                     FAPDFAVSM"
BASE COUNT          157 a          122 c          119 g          122 t
ORIGIN      
        1 caacagaaga atcaacaaca cctgcgccta caacaccatc aacagatcaa agtgttgata
       61 caggaaacgg cacaggaagt agtactccgg ctccaacgcc aacaccaaca cctgaacaac
      121 caaaacctgt aacacctgct ccagctcctt ctggaagtgt gaatggtgca gcaatcgtag
      181 cagaagctta caaatatatt ggtactccat atgtttgggg cggtaaagat ccaagtggct
      241 ttgactgctc agggttcaca cgctatgttt acatgcaagt aactggtcgt gacatcggtg
      301 gttggacagt tcctcaagaa tcagctggaa caaaaatttc agtttctcaa gcaaaagctg
      361 gagacttgtt attctggggg tcacaaggcg gaacttacca cgtagcgatt gctttaggcg
      421 gaggacaata tatccatgct cctcaaccag gcgaaagcgt aaaagttggt tcagttcaat
      481 ggtttgcccc tgactttgct gtcagcatgt aagaatgatt
//
LOCUS       DACDXE010000234          514 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.249, whole genome shotgun sequence.
ACCESSION   DACDXE010000234 DACDXE010000000
VERSION     DACDXE010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.249"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(107..>514)
                     /locus_tag="IWM23_003092"
     CDS             complement(107..>514)
                     /locus_tag="IWM23_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_465591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HAP9885640.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcc
//
LOCUS       DACDXE010000235          513 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.250, whole genome shotgun sequence.
ACCESSION   DACDXE010000235 DACDXE010000000
VERSION     DACDXE010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.250"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..132)
                     /locus_tag="IWM23_003093"
     CDS             complement(<1..132)
                     /locus_tag="IWM23_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAP9885641.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          185 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag aca
//
LOCUS       DACDXE010000236          510 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.251, whole genome shotgun sequence.
ACCESSION   DACDXE010000236 DACDXE010000000
VERSION     DACDXE010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.251"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..510
                     /locus_tag="IWM23_003094"
                     /pseudo
     CDS             1..510
                     /locus_tag="IWM23_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          212 a           88 c           92 g          118 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag
//
LOCUS       DACDXE010000237          508 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.252, whole genome shotgun sequence.
ACCESSION   DACDXE010000237 DACDXE010000000
VERSION     DACDXE010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.252"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          228 a           70 c           81 g          129 t
ORIGIN      
        1 aattccttag gcagcttttg ctgtgtagaa aggaataaat aataacgaaa gttcaaaagc
       61 taatgccgac tattctagac attaaagaag acgaaaacga acgataaaga aagaaaagtt
      121 atgaataatc gtttattgaa aaaaacttga aaaaatacaa aaaattcgtt gaccaagcta
      181 aatttagttg gtatattatt acttgtccaa aagacagaag aaaatattta ttcaaaaaca
      241 taacaaaaaa gttattgaca tgttactaac gacatgataa gataactgag tcgttaaaaa
      301 agaaccactt gcgaagcttg attctatcga aaaaaacttc aaaaaagttc ttgacaaata
      361 acaaacgata agttatgata aatgagttgc cgaaagattt cgaaaaacga aatcgaaaaa
      421 aacttttaaa aaagagttga caatcgcttg tctctctgat atgatataaa agttgctacg
      481 gcagccaaaa atgaatcggg aaacactc
//
LOCUS       DACDXE010000238          493 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.253, whole genome shotgun sequence.
ACCESSION   DACDXE010000238 DACDXE010000000
VERSION     DACDXE010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.253"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..360
                     /locus_tag="IWM23_003095"
     CDS             <1..360
                     /locus_tag="IWM23_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAP9885642.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          189 a           79 c          107 g          118 t
ORIGIN      
        1 cgaaaccaac cagttgttaa accaaatgca atgcaaatca ataactcatt gaagcgatgc
       61 tttgtgaaag cattggctct acacggactg ggattatatg tatttcaagg ggaagatatt
      121 ccaacaccac ctagaatcga tacaaagaaa ttaaacatgc tagagacgat tctagaagct
      181 ttcaatgagc agatgggtaa agatatgacc aaaaccttaa tcgaatatgt taatgagcag
      241 acagataaat tagggctctt agctgataac gttgaaacta ttgaacagtt aagctatgag
      301 caatgtgcct tgatggagcg agcaatagca gctaagagaa aagaattaga taagaagtga
      361 tatgagtgtt taaaccatta atcgattcat attcagcggt tctgaaaaag ttcaaaggaa
      421 aagacataag cgcaaccatc aatgaggaag tgaacattga tcgactaaag acgatgtatg
      481 acggctacga tgg
//
LOCUS       DACDXE010000239          484 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.254, whole genome shotgun sequence.
ACCESSION   DACDXE010000239 DACDXE010000000
VERSION     DACDXE010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.254"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(61..258)
                     /locus_tag="IWM23_003096"
     CDS             complement(61..258)
                     /locus_tag="IWM23_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAP9885643.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           67 g          170 t
ORIGIN      
        1 aattttttct accgacataa acaaaaaaac cacctcatat gaggcggtta acgttcaaca
       61 ttattttacg atatttactg cttgaggacc acgttgtcct tcttcaatat cataagtaac
      121 tgcttggcct tcatctaaag acttaaagcc ttctccttgg attgctgaga aatgagcgaa
      181 tacgtcattt ccatcttcac cagtgatgaa accaaaacct ttatctgagt taaaccattt
      241 tactgtaccg ttattcatat atatttcctc ctgatacgta tataatacgt gttttattgc
      301 aattaatatt acttggtaaa aggagttttt atagtgtgaa tatatcgctc agattacgtt
      361 tcaaattaga ttacttattt actataacac ggatgtaatg ctaacacaag gataatcact
      421 cgatatacgg tttaaatatt attatttgtc aaattaagcg agacaaaact ttccatttac
      481 gtaa
//
LOCUS       DACDXE010000240          479 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.255, whole genome shotgun sequence.
ACCESSION   DACDXE010000240 DACDXE010000000
VERSION     DACDXE010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.255"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          162 a           69 c           86 g          162 t
ORIGIN      
        1 acaaaagtat cttcgattaa tacttgctca agttatgtgc agctacgaaa aagtaggtac
       61 cgaggtactt tctttttcgc agcttttttt ggacttatga gaagtacatt cgctggaaga
      121 atacgatata aaacgagctt tttcactaaa gatattaata gagtgaaaga aacaagcttt
      181 tgttgtcagt tttttcactt ttctaaaaat gaaaataaaa aattctggaa gtggagaaga
      241 attttgcttg ctatactttg tcttgtagaa aagaggtaga caaaataatg caagctcaaa
      301 taagcgattt tccataacct tggtaggaat gacaaattaa ataggaaagg gaagtaataa
      361 tatgaataaa aaaaagttat ctctattcgg tttttttgcc ctgacagctt ccatggtttt
      421 gactgtttat gagtatccga cctttgcaac atcacaatta catttggttt tctttcttc
//
LOCUS       DACDXE010000241          460 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.256, whole genome shotgun sequence.
ACCESSION   DACDXE010000241 DACDXE010000000
VERSION     DACDXE010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.256"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(20..91)
                     /locus_tag="IWM23_003097"
     tRNA            complement(20..91)
                     /locus_tag="IWM23_003097"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IWM23_003098"
     tRNA            complement(113..200)
                     /locus_tag="IWM23_003098"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IWM23_003099"
     tRNA            complement(225..298)
                     /locus_tag="IWM23_003099"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IWM23_003100"
     tRNA            complement(312..382)
                     /locus_tag="IWM23_003100"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IWM23_003101"
     tRNA            complement(384..459)
                     /locus_tag="IWM23_003101"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DACDXE010000242          455 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.257, whole genome shotgun sequence.
ACCESSION   DACDXE010000242 DACDXE010000000
VERSION     DACDXE010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.257"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>455
                     /locus_tag="IWM23_003102"
     CDS             <1..>455
                     /locus_tag="IWM23_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885644.1"
                     /translation="ETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTDMNAAYFQL
                     TKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKKNWRFLLKN
                     RASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV"
BASE COUNT          150 a           95 c           90 g          120 t
ORIGIN      
        1 gaaacaggaa acttaattgc tgtcttgcct acgcgtaaat tacctcgatt aacaagctat
       61 ttcttaggct gcaccaatcc agaagaagta gaattcttag tcacagacat gaacgccgct
      121 tacttccagc ttaccaaacg tgttctgcca aatgcgaaag tagtgattga tcggtttcat
      181 attgtcaaac acatgaatca ggcactcaat gagttgcgta tccgtgaaat gaatgagctt
      241 cgtaaagcag gacagaaaag tcaggcagaa aaactgaaaa agaactggcg ctttctgcta
      301 aaaaatcgtg caagcatcaa ccattatgag tacaaaacat ggaaaagttt ccgagcacca
      361 aaatacccat ttctcactga agcaatgatg attgatcgat tgcttgagtt ttctgcgcct
      421 ctaaaggagg cgtatccctt ttttcatgaa ttagt
//
LOCUS       DACDXE010000243          452 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.258, whole genome shotgun sequence.
ACCESSION   DACDXE010000243 DACDXE010000000
VERSION     DACDXE010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.258"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>452
                     /locus_tag="IWM23_003103"
     CDS             <1..>452
                     /locus_tag="IWM23_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885645.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGST"
BASE COUNT          165 a           68 c           92 g          127 t
ORIGIN      
        1 ggtaagccga atttaatgtc caaactaaaa tcgagcgatt ttaacgttgg ttaccacggg
       61 tcactaactt cggataacga aaagctacat tttacttctg atggtacagg aagcattatt
      121 atgtttacgc gtattaatac acctcagctt gctagtggga aaacctatac tctgagtgcg
      181 aaagttcgat ttgatgaagg aactacagga gctattgata aattacgttt ggtgtatcgt
      241 acatcaccag gagaaaagat attattggaa gcaaatagta caaatattac aacagatgat
      301 gtagggaaag aaataacaat caaaggtaca gctaacgtta attatcaaat cacaaattta
      361 gatcgatttt atatgagtat tagctttgtt gacagggata aaataaatgg cggatttaag
      421 ttgtacgaca tcaaaatcga agaaggctca ac
//
LOCUS       DACDXE010000244          425 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.259, whole genome shotgun sequence.
ACCESSION   DACDXE010000244 DACDXE010000000
VERSION     DACDXE010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.259"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..193)
                     /locus_tag="IWM23_003104"
     CDS             complement(<1..193)
                     /locus_tag="IWM23_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885646.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREE"
BASE COUNT          156 a           70 c           47 g          152 t
ORIGIN      
        1 tttcttctct acctagaagg tagtctacag agactccaaa gtaatcagct aaccgagcta
       61 actcaataga attaggagtt tgtcttttcc aacttcctaa atatccattc gaatacccga
      121 aattcatttc gagctgtcta atggataatc ctttttcttt tgctaactcc tttattattt
      181 cgtaagtatt cattgataaa tcaacctttc tgaatgctta caaaaaaagt ttagataaat
      241 aatgtaaaaa tagttgactt atatacatta ttaatctata ctatgttttg taaacaagtt
      301 taacaactaa aaagacaaca aaaaatacta ttgattaata aatgccaacc gccaagaaag
      361 ctttttaaat caaagtttat atgtcttatt taactatgca tttagtatag attattaatc
      421 taata
//
LOCUS       DACDXE010000245          387 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.260, whole genome shotgun sequence.
ACCESSION   DACDXE010000245 DACDXE010000000
VERSION     DACDXE010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.260"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..198
                     /locus_tag="IWM23_003105"
                     /pseudo
     CDS             1..198
                     /locus_tag="IWM23_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          157 a           67 c           83 g           80 t
ORIGIN      
        1 aaagaagaaa tcgaacgctt tgaaaaaggt gaatcgactt tttcagctgc cacgatccga
       61 aaataccaac gaaaattgga aacattagaa cagctgaaag aaaaatcaga gaaaggaaaa
      121 gaaaatcttc ttcctgaagt acaagcgatc attgacagcg gacgagtgac gcaatggaaa
      181 aagaatccaa ctatctattt tctgaaaggc tatcgaaaag tcgctttaga actcaatgaa
      241 aacgggggat ttgaggaatc taaaaaatac caagccacgt cggaagaaga aaaggcagct
      301 gttcgtgaat tacttgaaga agctacacct actgattctg tgaaggagaa agaaaccgat
      361 catttctatc gagtagaatt caacgaa
//
LOCUS       DACDXE010000246          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.261, whole genome shotgun sequence.
ACCESSION   DACDXE010000246 DACDXE010000000
VERSION     DACDXE010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.261"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            16..354
                     /locus_tag="IWM23_003106"
     CDS             16..354
                     /locus_tag="IWM23_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAP9885647.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT          144 a           46 c           78 g           98 t
ORIGIN      
        1 agggaggaac agctaatgca atatctagaa gcaaaaatcc caattccaga aggctatgta
       61 attatctccc aagtggatta tgaggagtta aaaaaagctg atgatactgg tagatggatg
      121 acgttgccag aagtgctaga acggattaac agaaaatatg attggtttac ttctagagtt
      181 ttaaagaacc caagatatag aaatattatc gatatcgaga aaaacaaaaa tggatttgtc
      241 tattatccag ttgaaggaag agacacatat ctatttttaa gaagtaaaac acttgaattt
      301 ttagaaacaa atttttcgga aatcttaagg aggcaagcgg atgggaaaat ttaatagagc
      361 gctagt
//
LOCUS       DACDXE010000247          366 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.262, whole genome shotgun sequence.
ACCESSION   DACDXE010000247 DACDXE010000000
VERSION     DACDXE010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..366
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.262"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          145 a           47 c           45 g          129 t
ORIGIN      
        1 ttttacactc tctgattgac gtcataaaaa gtagctttga attcataatc gtttatataa
       61 cgattaatag aaaacaatat agtatttagt gttcatacaa tgattctaag atcattttga
      121 attaatcagt cttatagttg atttctaaat tgtcatatat tattaaaaat ttttgccatt
      181 taaaaagaaa agcgacagtt taccaataaa tttcaactga attatctaaa agtagatcag
      241 ttagtttaga taattcagtt gaaattgacc gactcaaatt acttgtataa tacctaggaa
      301 aaaattaact agcaccacgg gtatagttat ttatatttat aacaaataaa ccagagaaat
      361 agccta
//
LOCUS       DACDXE010000248          345 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.263, whole genome shotgun sequence.
ACCESSION   DACDXE010000248 DACDXE010000000
VERSION     DACDXE010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.263"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>345
                     /locus_tag="IWM23_003107"
     CDS             <1..>345
                     /locus_tag="IWM23_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885648.1"
                     /translation="YVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIK
                     QPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEF
                     VYQYTFETLQQL"
BASE COUNT          115 a           54 c           65 g          111 t
ORIGIN      
        1 ttatgtacga gtcgggaaaa aatggaatta tgtctgtttc attttggatc tgttcaatcg
       61 agaaattctc ggctattctt gtggagaaca taaagatgcc gttctagtaa aaaaagcatt
      121 tagccgtatc aaacaacctc tgacagaggt tgagattttt catactgatc gtggaaaaga
      181 gtttgataac caagctattg atgaattatt aacaactttt gacatcaatc gatcattgag
      241 tcataaaggc tgtccttttg ataatgccgt agctgaatca acttataagt cgttgaaagt
      301 agaatttgtc tatcaataca catttgaaac cttacaacaa ttgga
//
LOCUS       DACDXE010000249          344 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.264, whole genome shotgun sequence.
ACCESSION   DACDXE010000249 DACDXE010000000
VERSION     DACDXE010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.264"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            105..>344
                     /locus_tag="IWM23_003108"
     CDS             105..>344
                     /locus_tag="IWM23_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885649.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKD"
BASE COUNT          128 a           55 c           71 g           90 t
ORIGIN      
        1 agggaggtaa agtcgttgga agataaaatc attcttacaa aatcagagct tcaagaaatg
       61 ttaaacatcg agtatggaac gataattttt aggagggata ataagtgaaa aaaccaacac
      121 tttcagagct gatagaagct gctgagaagg cagtaaaacc agacgactgg taccgacaaa
      181 gtttaatctt ggagaagttc cacggcatgt caaaaactac tttagttgaa tactgcaagg
      241 aaatggaaac aattcctgaa tttgcagaag gaatacttcg tccaggtcac tcaaccacat
      301 ttattcatta ccatactttt atttggtttt taaagtggaa agac
//
LOCUS       DACDXE010000250          341 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.265, whole genome shotgun sequence.
ACCESSION   DACDXE010000250 DACDXE010000000
VERSION     DACDXE010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.265"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
BASE COUNT          112 a           64 c           56 g          109 t
ORIGIN      
        1 ctgttctaac attctcaact ttgtcagttt accataggaa aatgggaaaa tctacgaaag
       61 ctctcaacag aatcgtccta cttaaatacg cttttcttta ataataaata agttggttgt
      121 ttttgttatt tgcgctttca cttttcggaa taaataatgt gtatttatat ggaataaata
      181 aaaaaagagg tcgtgactaa aaacaagtca caacctctgg taaacggtgt tctcctgaca
      241 actcctcggt cacaagacac aatattcaac aaacatgaga agctattttt tgaaattgct
      301 ctttgtgact cagagtgaaa cggcaggctc acgacctctt t
//
LOCUS       DACDXE010000251          331 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.266, whole genome shotgun sequence.
ACCESSION   DACDXE010000251 DACDXE010000000
VERSION     DACDXE010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.266"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>331
                     /locus_tag="IWM23_003109"
     CDS             <1..>331
                     /locus_tag="IWM23_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6-like element ISS1D family transposase"
                     /protein_id="HAP9885650.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQ"
BASE COUNT          114 a           60 c           68 g           89 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag a
//
LOCUS       DACDXE010000252          325 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.267, whole genome shotgun sequence.
ACCESSION   DACDXE010000252 DACDXE010000000
VERSION     DACDXE010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.267"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..312
                     /locus_tag="IWM23_003110"
     CDS             <1..312
                     /locus_tag="IWM23_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885651.1"
                     /translation="NQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTN
                     LIEGFNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESM
                     F"
BASE COUNT          131 a           48 c           45 g          101 t
ORIGIN      
        1 aatcaaataa ggtttatgat agataaatgg aaaaagcagt atccacgagt ggttaaattg
       61 ctcatgaatc ctgctatatt aaccttttat aatttccctc cgtcaatcag aagaactatt
      121 tattcaacta atttgattga aggttttaac aaacaattaa agaaatatac caaaagaaaa
      181 gagcaatttc ctaatgaaga atctctggag agatttctag tatctcaatt caacaactat
      241 aatcaaaagt ttctatgccg agttcataaa ggttttaaag aaatacatga tacattagaa
      301 tcaatgtttt aacttgatca acaga
//
LOCUS       DACDXE010000253          321 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.268, whole genome shotgun sequence.
ACCESSION   DACDXE010000253 DACDXE010000000
VERSION     DACDXE010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.268"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..>321)
                     /locus_tag="IWM23_003111"
     CDS             complement(<1..>321)
                     /locus_tag="IWM23_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885652.1"
                     /translation="ITRHVWGDLMDEVEHLRLTDLNKSIYKKRKQTIERIFADAKEKH
                     GMRWTKYRGLEKVATHTMLVFAAMNLKKLATWLWKGKEPLFFCSKIRNEVDKKLFQAR
                     VTSL"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DACDXE010000254          320 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.269, whole genome shotgun sequence.
ACCESSION   DACDXE010000254 DACDXE010000000
VERSION     DACDXE010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.269"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            1..320
                     /locus_tag="IWM23_003112"
                     /pseudo
     CDS             1..320
                     /locus_tag="IWM23_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          128 a           62 c           59 g           71 t
ORIGIN      
        1 attcaaaaga attcaaacaa caaatcgtcg atctctatct cgctggtaag cctcgcgcag
       61 aaattattcg agagtatgag cttacgcctt cttctttcga taaatggatg aagcaagcac
      121 aatcaacggg ctcattcaaa gaaagagaca acttaacacc agaacaagca gaattgatcg
      181 cactaagaaa gaaaaataag caactcgaaa tggagaatga tattttaaag caagcggcgc
      241 tgatattcgg acgaaaagac aagtaattga tgccaacaag cataaatatt ccatatcagc
      301 gatgtgcaaa attctaaata
//
LOCUS       DACDXE010000255          311 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.270, whole genome shotgun sequence.
ACCESSION   DACDXE010000255 DACDXE010000000
VERSION     DACDXE010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..311
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.270"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            4..>311
                     /locus_tag="IWM23_003113"
     CDS             4..>311
                     /locus_tag="IWM23_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HAP9885653.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     T"
BASE COUNT          140 a           46 c           74 g           51 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgct tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaga agatgtttga acaccagaga
      121 aagaccaacg gtcagccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gaacgtttag aaaacaaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcggc
      301 ctggatatga c
//
LOCUS       DACDXE010000256          306 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.271, whole genome shotgun sequence.
ACCESSION   DACDXE010000256 DACDXE010000000
VERSION     DACDXE010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.271"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(76..>306)
                     /locus_tag="IWM23_003114"
     CDS             complement(76..>306)
                     /locus_tag="IWM23_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAP9885654.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           93 a           46 c           56 g          111 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaa
//
LOCUS       DACDXE010000257          300 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.denovo.272, whole genome shotgun sequence.
ACCESSION   DACDXE010000257 DACDXE010000000
VERSION     DACDXE010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..300
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.denovo.272"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..>300
                     /locus_tag="IWM23_003115"
     CDS             <1..>300
                     /locus_tag="IWM23_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAP9885655.1"
                     /translation="KDRKEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSL
                     IENQDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSI"
BASE COUNT          117 a           57 c           48 g           78 t
ORIGIN      
        1 aaagaccgaa aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa
       61 gaagcgaata ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg
      121 acgcagtcac tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt
      181 cgtagaacca tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac
      241 agccgtagaa aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt
//
LOCUS       DACDXE010000258         7887 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.104, whole genome shotgun sequence.
ACCESSION   DACDXE010000258 DACDXE010000000
VERSION     DACDXE010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7887
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.104"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            291..566
                     /locus_tag="IWM23_003116"
     CDS             291..566
                     /locus_tag="IWM23_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885656.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            579..2651
                     /locus_tag="IWM23_003117"
     CDS             579..2651
                     /locus_tag="IWM23_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAP9885657.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2816..4702
                     /locus_tag="IWM23_003118"
     CDS             2816..4702
                     /locus_tag="IWM23_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAP9885658.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4714..5661
                     /locus_tag="IWM23_003119"
     CDS             4714..5661
                     /locus_tag="IWM23_003119"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAP9885659.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5681..6208
                     /locus_tag="IWM23_003120"
     CDS             5681..6208
                     /locus_tag="IWM23_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAP9885660.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6271..6924)
                     /locus_tag="IWM23_003121"
     CDS             complement(6271..6924)
                     /locus_tag="IWM23_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAP9885661.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7056..>7887
                     /locus_tag="IWM23_003122"
     CDS             7056..>7887
                     /locus_tag="IWM23_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAP9885662.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     Y"
BASE COUNT         2689 a         1330 c         1674 g         2194 t
ORIGIN      
        1 tctgattcat cagtcccata aattatagag ccgatttttg tcgcagcccc atttttttgt
       61 tgtgataaaa gaacaagaaa ggcaggaatg ggaaagtaaa catccttttg tcagaaactt
      121 ttatctttct taagttttac ttgatatctg atactatttg gaatagaatg aggcgttgaa
      181 aaatttatgg aaatttctaa ctagcaataa acgatgaaac aatgaaatat ttttcatcca
      241 aaaagaactt tgctataatg atggaagttt gtgtatagga gatctggaaa atgagagaga
      301 gttttgaaca acaaaaaaaa ctgttgcatg atcgctatgg cgccctttcg atggatgacc
      361 gccgtcaaat actttgtaag ttgcgtaaaa gaaatatatt gatgtatcgt cagttggaac
      421 gtctgaaaca tgatctgctt cgtttggagt caaaacgtgt acagtgtgaa ttagagggga
      481 accaaacaca ggttgaagta gtcgaaacca aaattttaaa gaaaaaagaa caatttttaa
      541 aaatgcttac acaaaataaa aaataaaggg gatggacgat ggaatttgct gagttagtta
      601 ttgtatttgc agtaacgatc accttatcga acatagctag ccgcattttg ccaatgattc
      661 cagctccact gatccaaatt ttccttggag tggtattagg tctgacagag tggggacaat
      721 caatcgattt tgaacctgaa ttgttcttag ttatgattat tgcaccattg ttatttagag
      781 aaggagaaaa agcagatatt tcttcgattc tcaaaaattt cgggacaatt ttgttcttgg
      841 catttggagg agtcattttg acccttgtcg gggtaggtgc gacgttatca ttcctgttgc
      901 caagtgtccc acttgctgcc tgctttgctt ttggagcagc gttaggtccg acagatgcgg
      961 tagcagtcag ttctctttct ggacgagtga atatccctaa aaaggcgatg catattttgg
     1021 aaggagaagg tcttttgaat gatgcatctg gtgttactgc cttccagttt gctcttggtg
     1081 cgctgatcac cggatctttt tcagcagtaa acgcggggat gagtctagta gtttctagta
     1141 ttgggggagc tttgatcggc tttttgcttg tctggttcaa acaaaaaata attcatttga
     1201 tcgaaaaagc atcagcacaa gatgtcacag ggtatctgct gatcgaactt ttgcttcctt
     1261 ttctagccta tgtattggca gaggcatttg atgtatctgg aatcattgct gcagtggcag
     1321 caggtattct ccaagcttct ggttttagga aaatctctgt gtttgatgca gaattgtcga
     1381 gcttgtccca cagtacatgg acaactattg cttttacact gaatgcatta gtatttatat
     1441 ttttaggaat cgaattgacg caagtgttct cgccagtatg gggagacggt ttatatccaa
     1501 acggtctttt attagcgatt atcgtattaa tctctgtcat gctttttgtg atcagattca
     1561 tttctatcag tcttttttat gtattcaaag atggcagtaa aaagttcaaa aaacagttga
     1621 acgagatctt gatcttaaca tttggcggtg taaagggaac agtcagtttg gctacgattt
     1681 ttattttgcc attttcaatc aataatatga tgttttacca gcggtctttg cttttgtttc
     1741 tgacggcggg tgtcatctta gtgactttgg tcatagggat cattgtcttg cctatgctga
     1801 cggaaacaga ggaagcgaaa agcacggatt tgaatgcttt gatgatttta gaagaggtcg
     1861 ttgaagtttt gcgaaaagaa ataaaggaaa ttgatcgtaa tacaaaagaa tttttagcca
     1921 ctgaagcagt gatagaaaat tatcaagaac ggatacgtga tctgtacttg gaagatttaa
     1981 ccgatgatga aaagcaagaa gtacaagaaa tacaagcact gattttgtcg atcgaacggg
     2041 atggattgga tgaaagttat cgttcgggca agctttcttc taatggttat cgtttttact
     2101 cccgttttct ttctcgattc gaacattcta tcacaagcca gattttgtct tttatcggct
     2161 tttggttcat tgttgtccgt aggttaatgc gaatcgttct ccatccgaaa atgttttggc
     2221 aaagaagata tgctgatcgt cagacattta ttagtgatga agatatccaa gaaatcagag
     2281 agacatatca aaaaaatact cagttgatta ttgaaagttt agataacttg actgatattt
     2341 atgatgaaac gatggttcaa ttttttatcc agcaacgcaa acttgaagga ataaaaatga
     2401 actcggggaa tttgatttct tcgtggatga tccagcaaga tagtctgttt acgaaaaaaa
     2461 tgctaagagg ttattatctt gaaagaaaaa tcattgatga atacgaagtt gctgaaaaaa
     2521 tcacgacgtt ttctgcgaac agctatcgca ggaatatcaa tttgttagaa tcttatacga
     2581 tgaacaaacc atctgataac ttttctttcc gatttgcgtt tcgagcaaag tcaaaaaaac
     2641 gagtccaata aaataaaaag gtaaaaattt tcaagtttta taaaaaacaa aacatatcag
     2701 aacaggatcg aatcggtcct gttctttttt tgagaacaca aaaagaacaa ataccaaaaa
     2761 aattttcctg aagttcacta tttttatgat acaatatcca agataaggag ggaacatgtt
     2821 gaaagaatta aaagtaactg acctcaaaaa aacttatggt gaaaaagatt tgtttgacca
     2881 gatttctttt ttggtccacg aaaaagaccg tatcgggttg attgggacga acgggacggg
     2941 taaaacaagt ctcttaaata ttattgccgg tatcgatagc agcgacggtg atcggcaaac
     3001 agtgttttat ccaactgatt accgtattgg ttatctttct caagcagctg aattttccga
     3061 tgaattaact gttctccaag cagtctttca aggaaacagc cccttgatcc aaactgttcg
     3121 agcatatgag gaagcattga tcgaactagg ggaaaacgga gaagaccctg atgtgcaaaa
     3181 acgttatgct aaagcagaag aacaaatgaa taaagaagat gcttggacta cagataccaa
     3241 cgcaaagatt attttgcaaa aattaggaat cagtgaatta gataaaaaaa tcagtacttt
     3301 atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg ttgattgatg aacccgattt
     3361 actattacta gacgaaccaa caaaccattt agattatgaa gcgatcgagt ggttggaaaa
     3421 ctatttgaaa caataccgtg gcgcactatt gatggttacc cacgatcgtt attttctaga
     3481 tagagtagca aaccgaatct tcgaactttc atttggaaaa ttatacgagt ataaaggaaa
     3541 ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt gtagaagtag aacaagaaga
     3601 aaagcgcaag cggctttata aacaggaatt agcttggatg agggctggcg tacaggcacg
     3661 tggcaccaag cagcaagcac ggatcaaccg tttcgaagat ctaaaggaga acttgtacca
     3721 agtaaaccaa gaagatgatt tagagctgaa cctagcgaca caacgtttag ggaaaaaagt
     3781 actggaaatc aaagatggca gttaccgtat aaatgaccaa acactgctag aacatttaga
     3841 tttattgatt caatctcggg aaagactagg tattactggt aaaaatggtg cgggaaaatc
     3901 gacattgctt aatattctag ctggcagaat tcccctggat agcggtacaa tgtctatcgg
     3961 tgaaacggtt catcttgcgt attatacaca agaaaatgaa gaaatggcac cagataaacg
     4021 gatgattgct tacttacaag aagctgctga agaagcaaaa acagcagatg gcagtcaaat
     4081 cggagtagca gaattacttg aacgattttt atttccaaga tttatgcatg ggacactgat
     4141 aggcaaactt tcaggcggag aaaaacgccg gttatttttg ttgaaactgc tgattcagca
     4201 acctaatgtt ttattattgg atgaaccgac caatgatttg gacatagcaa cattgacgat
     4261 tttggaagat tattttagaa gttttccagg tgctgtgatc actgtttccc atgaccgtta
     4321 tttcttggac aaggtagcag ataagttatt agtattccaa ggaaacggaa aacaagaact
     4381 ttattacgga aacatgtcca gttatttact gaagcaaaaa gagacccagc agcctgctga
     4441 aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa aagaagaaat tatcttacat
     4501 ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca gaacttgaag aaaaaatctc
     4561 attgctccaa gaagagatga atcatcaagg agataatttc actcgattac aagaattgca
     4621 aaacgacgta tctgaaacag aagcacaact tgaagaaaaa atggcacggt gggaatactt
     4681 gagtgaatgg gtagaagatt aggaggaaaa aggatggaac aagcgtatct tgacttagga
     4741 agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa caggcacagg cacattaagc
     4801 ttattcggat accaaatgcg gtttgatctg caaaaagggt ttcctttgtt aacaacgaaa
     4861 cgagtacctt ttggattgat caaaagcgaa ttgctttggt ttttaaaagg agatacgaat
     4921 atccgctatc tattacaaaa taaaaaccat atttgggatg aatgggcatt tgaacgctac
     4981 atcaaaagtc cagactataa tggaccagat atgactgatt ttggccatag atgtttgaca
     5041 gatcctgaat tcaatgaaga atataaaaag cagagcaagc ttttttgcga gaagatccta
     5101 accgatgatt cctttgctga aacatacggc gatcttggac acatctacgg ctatcaatgg
     5161 cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa tcaaacaagt gatcgaagca
     5221 atcaaaaaga ctcctgattc tagaagattg atcgtttctg catggaatcc ggaagatgtt
     5281 cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat tttatgttca agagggacgg
     5341 ctaagctgcc aattgtatca acgcagtggg gatgtgtttc taggcgttcc gttcaacatt
     5401 gcaagttatg cgttgctgac tcacctaatt gcacatgaaa caggtttagc ggttggcgaa
     5461 tttgttcaca cattaggaga tgctcatctt tatatcaatc atatcgaaca aatgaaagaa
     5521 caattgagcc gagaaatacg cgcatttccg actttagttt tgaatcagga aaagcagtca
     5581 gtatttgatt ttgagatgga agatatccaa ttagaaggat atgacccaca tccggcgatc
     5641 aaagctccta tcgctgttta gaatcagaaa ggatgagaaa atgttagctg caatttgggc
     5701 acaggatgaa aacggattga ttggaaaaga agaccgtctt ccttggcgtt tgccaaatga
     5761 tctgaaattc ttcaaacaaa tgacagaagc aaatacacta gtgatgggac gaaaaacatt
     5821 cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg attgttttga cgagagattc
     5881 ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg gaagacgtgc ttgctctgga
     5941 aaaagaaaca gacggtatct ttttcatcgc tggagggtca gctgtttatc aagaatttct
     6001 gccattttgt acgatattat accgtacagt catccatcat gtattcgatg gagatgctta
     6061 ttttccacct gttgactggt ctgattggtc actgatcaat actagtcaag gagaaatgga
     6121 tgaaaaaaat cgttatccgc atcaatttga aacgtatcag cgtaatgaga atcctgtaaa
     6181 aagcgggaca ggcgaattga cgatataaaa aagctatgga tagactagct gttatttaga
     6241 gctgtcccat ccatagcttt atttttttta ttaagtaaag aataaaacag agaagtacat
     6301 aaaacctgca cctaacataa caaacaaatg ccaaacaaca tgcatatagc ggatagaatt
     6361 caagctataa aagaatgcgc ctatcgtgta agaaagcccg cctgctacca tcaaaataac
     6421 accagtcatt ccaagagagt gataaagagg aacagcagcc acgacacaga gccaccccat
     6481 aaaaatatag atgatcgtag atacttttga tactctctcc cgtttatgca acgtcaacgc
     6541 tttatatata atgccaagaa tcgcaaacaa ccagattaat acaaataaag tccaacctag
     6601 ccatcctcgg atactcagca aacaaaaagg agtataactt cctgcaatca aaagaaaaat
     6661 cgaactatga tcgaatactt ggaacacctt ttttgctttt gtaaaaatca atgaatgaaa
     6721 caaagtagac gttaaaaata aaagaatcat catcgatcca tagatagcat aagagacaat
     6781 atggagggag gaatgcaagt gggcaccttt tactaaaaga accactagtc ccgcgatact
     6841 caatccagtc ccaattccat gggtgactgc attaaacact tcatttaata tttcatacgt
     6901 tcgattaaat ttcgtttttt ccatgaaaac ggctccttta ctaggtgtaa ttaatgaaaa
     6961 atcattattt gtctgttata ctaaaaatag aaattgtctt acatgtagtt taacatgaat
     7021 aaggcatctt gttaaagaaa gagagagaga aaataatgac aaatgtaaaa atcgtaacag
     7081 attcttcatg tacgatggaa aaaaatgtac gcgatgcttt aggcattcgt gtcatgccgc
     7141 tatccatcat gatcgacgga acagtatatc ccgatgatga ccaactagac ggcgaaagat
     7201 ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac aagccaacca ccaattggag
     7261 aattcatctc gatgtatgat gagctaggag aagacggcag tgaagtgatt tcgatccata
     7321 tgacaaaagg cttgagtgga acagtagaag cagcaagaca agccagtaat ctaacaaaaa
     7381 cgaaagtaac ggttatcgac agtgatacca ctgaccaagg attatctttt caggtaattc
     7441 gagcagctga attagcaaaa gaaggtaaaa cagtagagaa aatccttgaa gaagtcaaaa
     7501 aagtacaaga caatacaaaa ttatacatcg gaatatccac actagataac ttggtaaaag
     7561 gtggaagaat cagccgaaca actggtttgc tgtctagtgt tttgaacata cgcgtggtga
     7621 tgaatttcgc ccattctgaa ttaatccctg taactaaggg gcgaggaaag aaaacgttcg
     7681 ataaatggtt taataatttg aaaaatgaac tgaaagaatt gccaaatgtg cgacaaatcg
     7741 gtatttcaca tgcggatgca aaagaattgg cgcaagaatt caaagagggt ttgcaagaat
     7801 tattcccgga catgcatatc cctgtactgc atacgaatcc ggttatcgct acgcatactg
     7861 gtaaagacgc gtttgcaatc atgtatt
//
LOCUS       DACDXE010000259         7104 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.110, whole genome shotgun sequence.
ACCESSION   DACDXE010000259 DACDXE010000000
VERSION     DACDXE010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7104
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.110"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..839
                     /locus_tag="IWM23_003123"
     CDS             <1..839
                     /locus_tag="IWM23_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAP9885663.1"
                     /translation="IRSLDVWSGKDLLQSEFIKSARQQLANYQLIVFLDYGFDMEMAR
                     RVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEIYHFDFLEAKELGLKHNSSFY
                     SKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKRDVTTNYFILPHRGNEQPGYL
                     TYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQKKLVTDNQAIAHYHFYRPENI
                     FLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKRFITNDSTIYEQYEYDLSSVE
                     NK"
     gene            complement(989..1930)
                     /locus_tag="IWM23_003124"
     CDS             complement(989..1930)
                     /locus_tag="IWM23_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAP9885664.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2056..3177
                     /locus_tag="IWM23_003125"
     CDS             2056..3177
                     /locus_tag="IWM23_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAP9885665.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYSLGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3435..4937
                     /locus_tag="IWM23_003126"
     CDS             3435..4937
                     /locus_tag="IWM23_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAP9885666.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5004..5531
                     /locus_tag="IWM23_003127"
     CDS             5004..5531
                     /locus_tag="IWM23_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAP9885667.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            5742..6803
                     /locus_tag="IWM23_003128"
     CDS             5742..6803
                     /locus_tag="IWM23_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAP9885668.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            6806..>7104
                     /gene="murQ"
                     /locus_tag="IWM23_003129"
                     /gene_synonym="yfeU"
     CDS             6806..>7104
                     /gene="murQ"
                     /locus_tag="IWM23_003129"
                     /gene_synonym="yfeU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="catalyzes the cleavage of the lactyl ether moiety
                     of N-acetylmuramic acid-6-phosphate (MurNAc-6-P) to form
                     N-acetylglucosamine-6-phosphate (GlcNAc-6-P) and lactate;
                     involved in MurNAc dissimilation pathway; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAP9885669.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIG"
BASE COUNT         2356 a         1247 c         1518 g         1983 t
ORIGIN      
        1 ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat
       61 cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat ggatttgata
      121 tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga
      181 atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg
      241 atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt
      301 cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat ctattttttg
      361 gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa
      421 gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc
      481 ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc
      541 gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt caaaagaaac
      601 tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt
      661 tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca tttaagccaa
      721 tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta
      781 acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa aacaaataaa
      841 aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct tatggcatca
      901 tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa ccagctgctg
      961 attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
     1021 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
     1081 gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
     1141 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
     1201 ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa gcaaattcag
     1261 atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca gcaatattac
     1321 aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg
     1381 aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag acttctacaa
     1441 ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
     1501 ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
     1561 agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta
     1621 aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac atatcatcta
     1681 attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt
     1741 cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca
     1801 acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag aggacctcat
     1861 ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc ccagctattg
     1921 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaat
     1981 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     2041 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     2101 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     2161 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
     2221 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
     2281 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
     2341 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttattcctta
     2401 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     2461 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     2521 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     2581 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     2641 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     2701 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     2761 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     2821 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     2881 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     2941 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     3001 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
     3061 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     3121 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     3181 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     3241 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     3301 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     3361 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     3421 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     3481 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     3541 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     3601 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     3661 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     3721 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     3781 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     3841 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     3901 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     3961 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     4021 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     4081 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     4141 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     4201 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     4261 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     4321 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     4381 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     4441 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     4501 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     4561 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     4621 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     4681 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     4741 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     4801 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     4861 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     4921 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     4981 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     5041 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     5101 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     5161 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     5221 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     5281 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     5341 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     5401 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     5461 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     5521 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     5581 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     5641 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     5701 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     5761 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     5821 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     5881 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     5941 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     6001 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     6061 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     6121 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     6181 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     6241 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     6301 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     6361 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     6421 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     6481 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     6541 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     6601 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     6661 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     6721 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     6781 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     6841 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     6901 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     6961 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     7021 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     7081 tgtagaaccg gaaatggtca tagg
//
LOCUS       DACDXE010000260         5788 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.125, whole genome shotgun sequence.
ACCESSION   DACDXE010000260 DACDXE010000000
VERSION     DACDXE010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.125"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            complement(<1..211)
                     /locus_tag="IWM23_003130"
     CDS             complement(<1..211)
                     /locus_tag="IWM23_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009296556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885670.1"
                     /translation="MSIPRMNYPQYRKARKLTHECCNYCDGNCLLLDDGEECICVQSI
                     SYSLLCRWFKAAVLPLDAALYAEITK"
     gene            complement(208..1536)
                     /locus_tag="IWM23_003131"
     CDS             complement(208..1536)
                     /locus_tag="IWM23_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAP9885671.1"
                     /translation="MAVTKIHPIKSTLKKALDYIENPDKTDGKLLVASFACSYETADI
                     EFEMLLAQAYQKGNNLGHHLIQSFAPGEATPEQAHEIGKQLAEEVLQGKYPYVLTTHI
                     DKGHVHNHIIFCAVDMVNQRKYISNKQSYAYIRRTSDRLCKENGLSVVKPGQSKGKSY
                     AEWDAQRKGTSWKAKLKAAIDAAIPQAKDFDDFLRLMQAQGYEIKPGKFISFRAPGQE
                     RFTRCKTLGEAYTEEAIKERIKGRVITRAPKERKGISLRIDLENNIKAQQSAGYERWA
                     KLHNLKQAAKTLNFLTEHGIDTYPDLESKVAEITAASDEAAATLKAMEHRLADMAVLI
                     KNISTYKQLWPVAMEYRNAADKAKFRREHESTLILYEAAAKALKGQGVKKLPDLYALK
                     AEYKRLAEEKERLYEKYGEAKKQMQEYGIIKQNVDGILRMTPGKEWTQEL"
     gene            complement(1497..1826)
                     /locus_tag="IWM23_003132"
     CDS             complement(1497..1826)
                     /locus_tag="IWM23_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAP9885672.1"
                     /translation="MANRKRKIVLRVPVTPEERAMIEQKMALLHTKNFSAYARKMLID
                     GYIVNMDTSDIRAQTAEIQKIGVNVNQIAKRLNGMGPVYAQDIEDIKGALAQIWQLQR
                     YILSSQR"
     gene            complement(2084..2455)
                     /locus_tag="IWM23_003133"
     CDS             complement(2084..2455)
                     /locus_tag="IWM23_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAP9885673.1"
                     /translation="MANIEDCPGFETFGADVKAARQAKRVSRKAMAEKINIDWRYLAN
                     IENDGAIPSLPVVIQLIKACGLPVERYFNPEIMREESELRQRVSHKLKLCPEEYLPII
                     EGAIDSAILIEKPKKNETEDV"
     gene            2600..2785
                     /locus_tag="IWM23_003134"
     CDS             2600..2785
                     /locus_tag="IWM23_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAP9885674.1"
                     /translation="MDYMTLKEAGEKWGVPPRWINYYCAAGRIPGAVKMATIWLIPKT
                     AEKPIDRRTKKGKAVTE"
     gene            2964..3110
                     /locus_tag="IWM23_003135"
     CDS             2964..3110
                     /locus_tag="IWM23_003135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885675.1"
                     /translation="MTEMENARNKDEEAAAVSAVYGRIEKAGIWILYGEVSRHWITAG
                     LMRL"
     gene            3728..4387
                     /gene="vanR"
                     /locus_tag="IWM23_003136"
     CDS             3728..4387
                     /gene="vanR"
                     /locus_tag="IWM23_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAP9885676.1"
                     /translation="MSIRILLVEDDDHICNTVRAFLAEAGYQVDACTDGNEAYTKFYE
                     NTYQLVILDIMLPGMNGHELLREFRAKNDTPILMMTALSDDENQIRAFDLEADDYVTK
                     PFKMQILLKRVEALLRRSGALAKEIRVGRLTLLPEDFTVLCDGTELPLTRKEFEILLL
                     LVQNKGRTLTHEIILSRIWGYDFESDGSTVHTHIKNLRAKLPENIIKTIRGVGYRLEE
                     S"
     gene            4390..5733
                     /gene="vanS"
                     /locus_tag="IWM23_003137"
     CDS             4390..5733
                     /gene="vanS"
                     /locus_tag="IWM23_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanB-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HAP9885677.1"
                     /translation="MERKGIFIKVFSYTIIVLLLLVGVTATLFAQQFVSYFRVMELQQ
                     TVKSYQPLVELIQNSDRLDIQEVAGLFHYNNQSFEFYIEDKEGSVLYATPNANTSNSF
                     RPDFLYVVHRDDNISIVAQSKAGVGLLYQGLTIRGIVMIAIMVVFSLLCAYIFARQMT
                     TPIKALADSANKMANLKDVPPPLERKDELGALAHDMHSMYVRLKETIARLEDEIAREH
                     ELEETQRYFFAAASHELKTPIAATSVLLEGMLENIGDYKDHSKYLRECIKMMDRQGKI
                     ISEILELVSLNDGRIVPIAEPLDIGRTVAELLPDFQTLAEANNQRFVTDIPAGQIVLS
                     DPRLLQKALSNVILNAVQNTPQGGEVRIWSEPGAEKCRLFVLNMGVHIDDTALPRLFT
                     PFYRIDQARSRKSGRNGLGLAIVQKTLDAMSLQYALENTSDGVLFWLDLPLTSTL"
BASE COUNT         1468 a         1347 c         1431 g         1542 t
ORIGIN      
        1 ctttggtgat ctcggcatac agggccgcat ccagcgggag gacagcggcc ttaaaccagc
       61 ggcacagaag cgaataggaa atgctctgta cgcaaataca ttcttcgccg tcgtccagca
      121 acaggcagtt gccgtcacaa tagttgcagc actcatgcgt cagcttccgg gccttgcgat
      181 attgagggta attcattcgt ggaatactca tagctcttgc gtccattcct ttcccggcgt
      241 catacgcaaa atgccgtcca cattctgctt gatgatgccg tattcctgca tctgcttttt
      301 cgcttcgccg tacttctcat acagccgttc cttttcctcg gccaatcgct tgtactcggc
      361 tttcagggcg tacagatcgg ggagcttttt cacaccctgt ccctttaggg cctttgccgc
      421 agcttcatac aaaatcaacg tgctttcatg ctcacgccgg aattttgcct tatccgctgc
      481 attgcggtat tccatagcca cgggccagag ctgcttgtag gtagaaatat tctttatcag
      541 caccgccata tccgcaagcc ggtgttccat agctttgagg gtagcggcgg cctcgtcgct
      601 ggcggcggtg atctcggcca ctttgctttc caaatccggg taggtgtcta tcccatgttc
      661 agtcaggaaa ttcaaagtct ttgcggcctg cttcaaattg tggagcttcg cccacctctc
      721 atagcctgcc gactgctggg ccttaatgtt gttttccaga tcaatccgca aagaaatccc
      781 cttgcgctct ttgggcgctc tggtgataac gcggcctttg atacgctctt tgatggcttc
      841 ctcggtgtag gcttccccca atgtcttgca gcgggtaaag cgttcctgtc cgggagcgcg
      901 gaaagaaata aactttcccg gcttgatctc atagccctgc gcctgcatga gccgcaggaa
      961 atcatcaaaa tcttttgcct gcgggatagc ggcgtcaatg gctgctttga gcttcgcttt
     1021 ccagctcgtc cctttccgct gggcgtccca ctcggcatag ctcttgccct tactctggcc
     1081 gggcttcacc acggacaatc cgttttcctt gcacagcctg tcgctggtgc gccggatata
     1141 ggcatagctc tgtttgttgg aaatgtactt gcgctgattt accatatcca cggcacagaa
     1201 aataatgtga ttgtggacgt ggcccttatc aatatgggta gtcagcacat agggatattt
     1261 cccttgcagc acttcctcgg caagctgctt cccgatctca tgggcctgtt ccggcgtggc
     1321 ttcgccgggg gcaaaggatt gtatcaggtg gtggcccaga ttattgccct tttggtatgc
     1381 ctgcgccagc agcatttcaa attcaatgtc cgctgtttca taagagcagg caaaggaggc
     1441 caccagcagc ttgccgtcag ttttgtccgg gttctcgatg tagtcaaggg cttttttcag
     1501 cgttgacttg ataggatgta tctttgtaac tgccatatct gcgccagcgc cccctttata
     1561 tcctcgatgt cctgtgcgta caccggcccc ataccgttca gcctctttgc aatctgattg
     1621 acgttgacgc cgatcttctg tatctcggcg gtctgcgccc ggatgtcgct ggtgtccatg
     1681 ttgacgatat agccgtcaat gagcatcttc cgggcgtagg ctgaaaagtt cttcgtatgc
     1741 agcagcgcca ttttctgttc aatcatagcc cgttcctccg gcgtcacagg gacgcgcagc
     1801 acgatttttc gctttctgtt tgccatgctt caccgtcctt tccattcagg ggtttgggga
     1861 tttccccaac aagcaaaacg gacgcggccc ggcagggacg gggcaggccg tttttccggg
     1921 agtggctaca ctccctatgc ttgctacgtt actgtttctc cactaacaag acgtttccaa
     1981 agccccaaac tacccgcaaa atagccctct ggaaagaaaa aagtgcaaaa aaatagaagc
     2041 tacgctgtgc agctcccgct aaaagtaaaa cagaggatgc cagttacaca tcctccgttt
     2101 cattcttctt cggcttttca attagtatgg cgctgtcaat cgcgccctcg atgatcggca
     2161 ggtattcttc ggggcagagt ttcagcttgt ggctgaccct ttgccgcagt tcgctttcct
     2221 cccgcatgat ctccgggtta aagtagcgtt ccacaggaag cccgcaggct ttaataagct
     2281 gtatgacaac cggcagactg ggaatggccc cgtcgttttc gatgttggca agatagcgcc
     2341 agtcaatatt tattttctct gccattgctt tgcgcgaaac gcgctttgct tgccgggccg
     2401 ctttcacatc cgcgccgaaa gtttcaaagc cgggacaatc ttcaatattc gccatatcgc
     2461 acctcccact tacattgtat atttcatact ctgcccgtgg aatgttggta tatgcacctt
     2521 tcttaaagtt tatatttcac attttgtttc ttttattctt cgagcaaaag aattataatg
     2581 taccctgtgg aggtgtacta tggactacat gacgttgaaa gaggcaggcg agaaatgggg
     2641 tgtgccccca cgatggataa actactattg tgctgcggga cgcatccccg gcgctgtaaa
     2701 gatggcgacg atctggctca ttcccaaaac tgcggaaaag ccgattgaca ggcgtacaaa
     2761 gaaaggaaag gcggttactg aatagtataa tgaaaccggc gcattttgga tatggttttg
     2821 aagagagaga aagcatggta ttttgtcacg gaaaaactat aagatgatag atggcaggct
     2881 gctccagacc gataaaaagt attctcatct gaaacagaaa gaaaagattt acacatggat
     2941 gtatgaagaa gcgaaacttt atcatgacag aaatggaaaa tgcccgaaac aaagacgagg
     3001 aagctgctgc tgtatcagcc gtatatggta gaattgaaaa ggccggcatc tggattcttt
     3061 atggtgaagt ctccagacat tggataactg ctggtctaat gcgcctttga gtatggggtg
     3121 attgatttta ataccgggga ggtacggctt tttgaggaaa atattctggg atttacattt
     3181 ggcgattatt acatttcctg taactgcaag gacacggatg atgaagatat gaggaatata
     3241 gacttgctga ttttctactg tcttgaacgg tattaggact gaatctgctg cgcggagtgc
     3301 ggcaagagcg accggaggga gtacacacag gccctgcctg tgtgaaaagc cataccgcaa
     3361 ggtgcattcg caaaaaagag gccgcgcatt ttgctgaggc ttggggtgtc cccaacaagc
     3421 aaaacgagaa cgccgcaggc gggcaagtgg tggatcagaa tatccgtaaa ggatattctg
     3481 atccattgct tgccagtatt atcgggatgt tttgacaacc gctgtaatcg aaacatccca
     3541 aacattaaga aggtgttaat tattattatc ttgatttaat tcgtttaaac caataatata
     3601 gatacataca ccatgaaaag aagcgagtat cccaatgacg gatgattaaa atccgaaaag
     3661 aaatcgcttt gaaactacag ggaaactaca gactgttatg ttatcttctt aaaatggagg
     3721 gatttttatg tcgatacgaa ttctacttgt cgaggatgat gatcatatct gtaatacagt
     3781 aagggcgttt ctggctgagg caggatatca ggtggatgcc tgcacagatg gaaatgaggc
     3841 atacaccaag ttttacgaaa acacttatca actggttatt cttgatatta tgctgcccgg
     3901 tatgaacggg catgaacttt tgcgtgaatt tcgtgcgaaa aatgatactc ccattctgat
     3961 gatgacagcc ctgtcggatg acgaaaacca aatccgggcg tttgatctag aggcagacga
     4021 ctatgtaaca aagccattca agatgcagat tttactaaag cgggtggaag ccctgctgcg
     4081 gcgcagcggt gcgctggcaa aggaaatccg tgtcggcagg ctgacacttc tgccggagga
     4141 tttcacggta ctttgtgacg gtacagagct gcccctgaca cgaaaagaat ttgaaatcct
     4201 tttgctgctg gtgcagaaca aaggcagaac cttaacgcat gaaatcattt tgtcacgtat
     4261 atggggatat gactttgaga gtgatggcag cacagtccac actcatatca aaaatttgcg
     4321 ggccaagctg ccggaaaata tcatcaaaac cattcgcggt gtaggatacc gattggagga
     4381 atcataataa tggaaagaaa agggattttc attaaggttt tttcctatac gatcattgtc
     4441 ctgctactgc ttgtcggtgt aacagcaaca ctgtttgcac agcaatttgt gtcttatttc
     4501 agagtgatgg aattacagca aacagtaaaa tcctatcagc cattggtgga actgattcag
     4561 aatagcgata ggcttgatat tcaagaggtg gcagggctgt ttcactacaa taaccaatcc
     4621 tttgagtttt atattgaaga taaagaggga agcgtactct atgccacacc aaatgccaat
     4681 acatcaaata gttttagacc cgactttctt tatgtggtac atagagatga taatatttcg
     4741 attgttgctc aaagcaaggc aggtgtggga ttgctttatc aagggctgac aattcgggga
     4801 attgttatga ttgcgataat ggttgtattc agccttttat gcgcgtatat ctttgcgcgg
     4861 caaatgacaa cgccgatcaa agccttagcg gacagtgcga ataaaatggc aaacctgaaa
     4921 gatgtaccgc cgccgctgga gcgaaaggat gagcttggcg cactggctca tgatatgcat
     4981 tccatgtatg tcaggctgaa agaaaccatc gcaaggctgg aggatgaaat cgcaagggaa
     5041 catgagttgg aggaaacaca gcgatatttc tttgcggcag cttctcatga gctaaaaacg
     5101 cccatcgcgg ctacaagcgt tctgttggag ggaatgcttg aaaatatcgg tgactacaaa
     5161 gatcattcta agtatctgcg cgaatgcatc aaaatgatgg ataggcaggg caaaatcatt
     5221 tccgaaatac tggagcttgt cagcctgaat gatgggagaa tcgtacccat agctgaaccg
     5281 ttggacatag ggcgcacggt tgccgagttg ctgcccgatt ttcaaacctt ggcagaggca
     5341 aacaaccagc ggttcgtcac agatattcca gccgggcaaa ttgtcctgtc cgatccgagg
     5401 ctgctccaaa aggcactatc caatgtcata ttgaatgcag ttcagaacac gccgcaggga
     5461 ggcgaggtac ggatatggag tgagcctggt gctgaaaaat gccgcctttt tgttttgaac
     5521 atgggcgttc acattgatga tactgcgctt ccaaggctgt tcaccccatt ctatcgcatt
     5581 gatcaggcgc gaagcagaaa aagtgggcga aacggtttag gacttgccat cgtacaaaaa
     5641 acgctggatg ccatgagcct ccagtatgcg ctggaaaaca cctcggatgg cgttttgttc
     5701 tggctggatt taccgctcac atcaacattg taaatattta aaacttaaat gattttgacc
     5761 gacaggtata accctgccgg tctttttt
//
LOCUS       DACDXE010000261         3551 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.149, whole genome shotgun sequence.
ACCESSION   DACDXE010000261 DACDXE010000000
VERSION     DACDXE010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.149"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..987
                     /gene="rlmN"
                     /locus_tag="IWM23_003138"
     CDS             <1..987
                     /gene="rlmN"
                     /locus_tag="IWM23_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAP9885678.1"
                     /translation="AAQVWEWLYQKRVSNFTEMTNLSKQLIEKLSEHFIINPLKQMVV
                     QEASDGTVKYLFELPDKNMIETVLMRQEYGLSVCVTTQVGCNIGCTFCASGLLKKNRD
                     LTAGEIVAQIMMVQHYFDERNLGERVSHVVVMGIGEPFDNYDNVMDFLHIINDAKGLA
                     IGARHITVSTSGLAHKIKEFANNGLQVNLAISLHAPNNEVRTSIMRINRSFPIEKLME
                     AVDEYLEKTNRRITFEYIMLNQVNDRPEHAQQLADLLKDKKKLAYVNLIPYNPVSEHD
                     QYSRSPKADVLRFYDVLKKNGVNCVIRKEHGTDIDAACGQLRSKQMKKTVAK"
     gene            complement(1180..2283)
                     /locus_tag="IWM23_003139"
     CDS             complement(1180..2283)
                     /locus_tag="IWM23_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAP9885679.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            2836..3384
                     /gene="aac(6')"
                     /locus_tag="IWM23_003140"
     CDS             2836..3384
                     /gene="aac(6')"
                     /locus_tag="IWM23_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAP9885680.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1155 a          617 c          714 g         1065 t
ORIGIN      
        1 gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg
       61 aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa
      121 cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat
      181 aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg
      241 acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac
      301 cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat
      361 gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt
      421 gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca
      481 attggtgctc ggcacattac agtttccact agtggattag cacacaaaat caaggaattc
      541 gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg
      601 cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg
      661 gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa
      721 gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa
      781 ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc
      841 agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt
      901 gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag
      961 caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa
     1021 aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc
     1081 gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa
     1141 tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc
     1201 ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt
     1261 ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa
     1321 gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg
     1381 gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa
     1441 taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag
     1501 aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa
     1561 aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga
     1621 gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat
     1681 gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc
     1741 agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt
     1801 atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg
     1861 aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg
     1921 cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat
     1981 gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg
     2041 gacactttca tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca
     2101 gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt
     2161 tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc
     2221 atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact
     2281 catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct
     2341 gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg
     2401 gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg
     2461 aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt
     2521 tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc
     2581 aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag
     2641 ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta
     2701 taattttttt attttataag taagataaat aatgaaagaa tagagatact gattggtagc
     2761 taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa
     2821 caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat
     2881 cagctttctg atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa
     2941 gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag
     3001 ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca
     3061 ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta
     3121 gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac
     3181 catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc
     3241 atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta
     3301 ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt
     3361 attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag
     3421 aaataagaag gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc
     3481 cgaagaagct acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg
     3541 agtagtaaga a
//
LOCUS       DACDXE010000262         3356 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.150, whole genome shotgun sequence.
ACCESSION   DACDXE010000262 DACDXE010000000
VERSION     DACDXE010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.150"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            315..812
                     /gene="dfrG"
                     /locus_tag="IWM23_003141"
     CDS             315..812
                     /gene="dfrG"
                     /locus_tag="IWM23_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAP9885681.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(884..2836)
                     /locus_tag="IWM23_003142"
     CDS             complement(884..2836)
                     /locus_tag="IWM23_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAP9885682.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2843..3139)
                     /locus_tag="IWM23_003143"
     CDS             complement(2843..3139)
                     /locus_tag="IWM23_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAP9885683.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1039 a          576 c          552 g         1189 t
ORIGIN      
        1 gtaaatgtag tactccacta taaaggcgta tatatgcaag taagtagttc agttttggag
       61 tacaaaatga caaaatgtac ttcaaaatgg actacttttt tcattttaac ttacatacca
      121 aattacatac aaaatgattt ataaaatgca attgaaaatg ataattgaat gaaaatttgt
      181 gattttgtgg tattatgata atataactta tagaaaaaat tgcttattca actaacgggc
      241 aggataatga gaaaaggttt tttgatagaa ataagaaatt agatggtaaa ttagttaaaa
      301 aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat ggataagaat agagtgattg
      361 gcaaagagaa tgacattcct tggaggattc ccaaggactg ggaatatgtt aaaaatacta
      421 caaagggaca tccgataata ttaggtagga agaaccttga atcaatcgga agagccttac
      481 ctgacagaag aaatattatt ctgacgagag ataaggggtt tacctttaat ggttgtgaaa
      541 ttgttcattc aatagaagat gtttttgagt tatgtaaaaa cgaagaagaa atttttattt
      601 tcggaggaga acagatttat aatttgtttt tcccttatgt tgagaaaatg tacatcacaa
      661 aaatacatca tgaattcgaa ggagatactt tttttccaga agtgaattat gaggaatgga
      721 atgaggtatt tgcccaaaaa gggataaaga atgataaaaa tccgtataac tactattttc
      781 atgtatatga aagaaaaaac ttattgagtt aacgggtgcg ttaattgaag aagaacagta
      841 aagataaagc acaagagctt gggattactc caagctcttt ttgtcatttg aaataaatta
      901 tatctttcca atcaaattgt ttatctgtaa tccctaatct ttgagcaggt gtcaaaacct
      961 ttttatccgc tgatttaaaa gggcgacaaa agttgtagta agttcttaga attgtaatcg
     1021 caaattgagc atacttcgga ttgaagttag cgtaaatata gctcttctta tcaccacgag
     1081 ctgttgttaa tggtctttca agagatgata tataacgtct tatctgttga ataaagctat
     1141 tggtcgaatg gtcattaaca tttaatagca tctttgcgat ttcgtttggt tccagagcag
     1201 ataaatccgt tctacaatca actgaataaa atcctttatc cttagtggct aatggatgtt
     1261 tgatagggtt atcggcatat ttgcgatatc tttctccatc tttactaata gcctcttcgt
     1321 ggaattgatg acttgttagt aattcagtta gataatgaga tgccaaagtc cgtagaaact
     1381 ttgttttgaa tcctctgata tctccccaat cgagcagttc tgcttttgct tgtccgaatt
     1441 cttttaagca ttgttttctg cttttcgtct tgttaacttg gcaaataaaa tgatgtgcat
     1501 cgctcaacct taattctttc gtgaatactc tataaaaggc gtttctaata gagaagtcat
     1561 catcactaat cattcgccat tcgtcggcat taaccatttg tttaattaac caatagtgag
     1621 cagttgtagt ataaggagca tcaacattta aaccatcgac aaatctactc ctattagtaa
     1681 ttttccctaa ttcgtgaaga taagcattcc tattttcgct atcgttagcg gatggttctt
     1741 gtggataata agaccaatct aacctatcat ttttacgact aaaagtattg agatggtctt
     1801 ctttgaattt tcttgtatct tcatttaatt catccattga aatattccaa tcataggcaa
     1861 catcagaacg gaacacatat cttgataaga cctcagcaga tacaactata tacgtttgca
     1921 tattaaggtc ttcaaacccc gtatatttct ttgaaccttg tcctttctta cggacgttat
     1981 ttaggtagta gtgcatcttg tccgtattaa gccacatctc gttaaatttc tttgtttgaa
     2041 ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg cctatacagc cactccagct
     2101 tatgatagta ggttccaact ccaattccta aaatatcaca agtacgactg actgaaaccc
     2161 ttccaacaac catcttagcg aacattggaa gaatggtgtt cttttgttgg tgataagtag
     2221 tggtttcttc tctttttggt agaacattag tgaacttctt acacgcctta cattgatagc
     2281 gttgagattt gcccctactt ttcccccttt tgtaaaattg cttcggctca ttgaatgggg
     2341 ttgattcttc ttccgagcag ccttctttat gaaattgata atctggctca acatcttgga
     2401 tgctattgat ttctataagg cgtttaattt cttcaacaac agaccaatta gataaagggg
     2461 ttactgtaca gttttgcgat acacctctgc caataggatt gtcattgcag tacatacctt
     2521 tatgaccttt atctttggaa ctaccagtca ttttatatcg acttggtttc ccttttacat
     2581 ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa cggattgatg cagtaattat
     2641 attgaatggg atattcagaa ccgttccatt taaaagacgt tggcaagaac aacattttgt
     2701 tataccttgt tgcaaatcgt tttggagtta ataaatcata ttgtttagcc ctatctttta
     2761 attcttcgtt tgaaatcgga attttaacat ctacaatttc atcttccttc gtggctaatc
     2821 ttttaagctt ggtcattgtc tatcaaccca ctctatcttt gatacccatt ttttcaacaa
     2881 tccgcttatt acttcgcttg ttggcaatcc attcaataaa cttcttatcg tctaaaatgt
     2941 tcaataagaa ttcatccact gcttcgcttt cagtacgctc tgcatattca gcgtattgct
     3001 taactatcac ccttacttgc tctgatattt cccaatccac agacttagcg ttcttatttt
     3061 ttggctcaag aaattgcata ggaaaatgca cccctttttg cttagtaaat tgtaaccatt
     3121 ttaacacacc aatttgcatt cctacaataa ttatttcacg ttcgcatttt tatatacatt
     3181 ttaacttaca ttttgtggtg taaattaaca acaaacgttg atttaacaat aaaaaacgag
     3241 tatcaaattc agttgcaatt tgatactcat ttaggtttac attttcactt tttgtactcc
     3301 aaaactgaac tacttaatat gcaagacgtt gtcttttttt tgtatattca attttg
//
LOCUS       DACDXE010000263         3245 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.154, whole genome shotgun sequence.
ACCESSION   DACDXE010000263 DACDXE010000000
VERSION     DACDXE010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.154"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            119..901
                     /locus_tag="IWM23_003144"
     CDS             119..901
                     /locus_tag="IWM23_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HAP9885684.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(1027..1758)
                     /gene="erm(A)"
                     /locus_tag="IWM23_003145"
     CDS             complement(1027..1758)
                     /gene="erm(A)"
                     /locus_tag="IWM23_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HAP9885685.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(1816..1903)
                     /locus_tag="IWM23_003146"
                     /pseudo
     CDS             complement(1816..1903)
                     /locus_tag="IWM23_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017771940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ErmCL family antibiotic resistance leader
                     peptide"
     gene            2267..2929
                     /locus_tag="IWM23_003147"
     CDS             2267..2929
                     /locus_tag="IWM23_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAP9885686.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            3164..3245
                     /locus_tag="IWM23_003148"
                     /pseudo
     CDS             3164..3245
                     /locus_tag="IWM23_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
BASE COUNT         1077 a          472 c          599 g         1097 t
ORIGIN      
        1 ttaaacgcta acggtcagct ttattgaaca gtcatttagg tatatgtcca atctagggta
       61 agtaaattga gtatcaatat aaactttata tgaacataat cagttacgag gtgaaatcat
      121 gagcaatttg attaacggaa aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa
      181 agatttattt ggaagttcaa tagttggagt atatctattt ggttcagcag taaatggtgg
      241 tttacgcatt aacagcgatg tagatgttct agtcgtcgtg aatcatagtt tacctcaatt
      301 aactcgaaaa aaactaacag aaagactaat gactatatca ggaaagattg gaaatacgga
      361 ttctgttaga ccacttgaag ttacggttat aaataggagt gaagttgtcc cttggcaata
      421 tcctccaaaa agagaattta tatacggtga gtggctcagg ggtgaatttg agaatggaca
      481 aattcaggaa ccaagctatg atcctgattt ggctattgtt ttagcacaag caagaaagaa
      541 tagtatttct ctatttggtc ctgattcttc aagtatactt gtctccgtac ctttgacaga
      601 tattcgaaga gcaattaagg attctttgcc agaactaatt gaggggataa aaggtgatga
      661 gcgtaatgta attttaaccc tagctcgaat gtggcaaaca gtgactactg gtgaaattac
      721 ctcgaaagat gtcgctgcag aatgggctat acctctttta cctaaagagc atgtaacttt
      781 actggatata gctagaaaag gctatcgggg agagtgtgat gataagtggg aaggactata
      841 ttcaaaggtg aaagcactcg ttaagtatat gaaaaattct atagaaactt ctctcaatta
      901 ggctaatttt attgcaataa caggtgctta cttttaaaac tactgattta ttgataaata
      961 ttgaacaatt tttgggaaga ataaagcgtc ctcttgtgaa attagagaac gctttattac
     1021 tttaatttag tgaaacaatt tgtaactatt gaaaatagaa agaaattgtt ccttcgatag
     1081 tttattaata ttagtgacat ttgcatgctt caaagcctgt cggaattggt ttttagtgaa
     1141 aagaacacga tattcacggt ttacccactt ataaacaaaa gatcgatact ttttgtagtc
     1201 cttctttgaa atcaatggtt gatgtcgttc aagaacaatc aatacagagt ctacacttgg
     1261 cttaggatga aaatatagtg gtggtacttt tttgagcatt tttatatcca tctccaccat
     1321 taatagtaaa cccaaagctc gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac
     1381 gataagatag ctatatttag cctgactttc aaaggtaatt cttttgacaa tatccgtact
     1441 gatgttataa ggaatattac catatatctt atagtttata tgttttggga aggaaaattt
     1501 tagaatatcc gtttgaatca cttttatatt ctcagagggg tttaccgctt ctttagtcac
     1561 ttgacataag cctccatcaa tttctatagc agtaactgat cgactcattt tgactagctc
     1621 tttggtaaaa tgtccttttc ctgatccgat ttctattacg ttgtcttgtt tactgatatt
     1681 cgtgtgattc aatatttctt ttacatgctt tttagaagta ataaaatttt gcgtgtcttt
     1741 agggtttttc tggttcatta taaccttctc cttactggtt ataatgaact gacattaagc
     1801 gttcattata accaattaat tttgatttgg ttgataacga actttattaa taacaaatat
     1861 agaaaaagta cccatacttt tatctccttt ccattttttt cattatgaat gagataaagt
     1921 aatttctact actgcgatac tggtgcacat aattgaaacc tccttatgta actgaatatg
     1981 attgtatcat cttttcattt tagaataaac ctttatatgt aattttgcac ttgacacttc
     2041 agatggacgt tttcttcaaa ataatatgtt ttgaaacttg ataagagaag ccactgtctt
     2101 tactaaaaag taaaggctgc gataaaggac agtgacttta ataaagttgc actgttgtat
     2161 tgtctttgga taaaattgtc taaagggaag ttttatcccc aacgaaaaaa catctaaagt
     2221 ggtaagtaat gaaagaatgt cgataaagta ggaatggggg atctttttga atacaataga
     2281 atcacagttt gataaggttg cagaagatta cgatttcgtg aatgagcttt tgaatgatta
     2341 ttcattcttt gtgtctaata tgtctccaaa gaaaggcaga gcattagata tcggatgtgg
     2401 ctcgggtttg ttagtggaga aattagcaag ttattatgat gaagtggtag ggattgatat
     2461 ttctaatcaa atgctcgatc ttgccaaatc taaacgtcaa ctaacaaata cggtctatct
     2521 gaatatgaat gcagaacaac ttaattttaa tgagaagttt gattttattg taagccgaac
     2581 aacctttcat catttggatg atatagccag cgtaatacag caaatgaagg aactgttgaa
     2641 tgaggaagga agaatagtta ttcttgataa tgtatctgaa gtcgagacac cgcctaccta
     2701 tgtttataaa ttaggggcaa tccaagagtt tttaccgcat tgtttcaaat ttggaataaa
     2761 aaatgcgatt agaatttata atcacaacac atctaaatca tggcttgagc atttagcttc
     2821 agataagtac ctatctgaac aaaattatta cgatttatat gaaaagttgt tgcctggatg
     2881 ccaattccat aaaatgggtt gggcaatggg ggttgtctgg acaaaataac ttaaagctgt
     2941 ggctttgaaa agttgaattt gtgattgttt tcagagctat cgggtgcttt agtataaata
     3001 acaatgaata aaaaatcagc ctgtatctta tgttactatt aagtaattta agatacaggt
     3061 tttactattt ttgaaataag taccttccac ttcaaataca tgtattcaaa gtgaaagtat
     3121 tgatttaaat gattcttttg taatttactc ttgataatat gaaattccat cgcagcagga
     3181 tatgaatttt accaaaagga tacaaaagtg tggagaaatg atggggatca ctgtactgga
     3241 ccact
//
LOCUS       DACDXE010000264         2800 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.160, whole genome shotgun sequence.
ACCESSION   DACDXE010000264 DACDXE010000000
VERSION     DACDXE010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.160"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            47..1018
                     /locus_tag="IWM23_003149"
     CDS             47..1018
                     /locus_tag="IWM23_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-B"
                     /protein_id="HAP9885687.1"
                     /translation="MRKSVGITVFGCEQDEANAFRALSPDFHIIPTLISDAISADNAK
                     LAAGNQCVSVGHKSEVSEATILALRKVGVKYISTRSIGCDHIDTTAAERMGISVGTVA
                     YSPDSVADYALMLMLMAIRGAKPTMHAVAQQDFRLDRIRGKELGDMTVGVIGTGHIGQ
                     AVVKRLRGFGCHVLAYDNSRKMDADYVQLDELLKNSDIVTLHVPLCADTRHLIGQKQI
                     GEMKQGAFLINTARGALVDTGALVEALESGKLGGAALDVLEGEDQFVYTDCSQKVLDH
                     PFLSQLLRMPNVIITPHTAYYTERVLQDTTEKTIRNCLNFERSLQHE"
     gene            1011..2039
                     /gene="vanB"
                     /locus_tag="IWM23_003150"
     CDS             1011..2039
                     /gene="vanB"
                     /locus_tag="IWM23_003150"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanB"
                     /protein_id="HAP9885688.1"
                     /translation="MNRIKVAIIFGGCSEEHDVSVKSAIEIAANIDTEKFDPHYIGIT
                     KNGVWKLCKKPCTEWEADSLPAILSPDRKTHGLLVMKESEYETRRIDVAFPVLHGKCG
                     EDGAIQGLFVLSGIPYVGCDIQSSAACMDKSLAYILTKNAGIAVPEFQIIDKGDKPEA
                     GALTYPVFVKPARSGSSFGVTKVNGTEELNAAIEAAGQYDGKILIEQAISGCEVGCAV
                     MGNEDDLIVGEVDQIRLSHGIFRIHQENEPEKGSENAMITVHADIPVEERNRVQETAK
                     KVYRVLGCRGLARVDLFLQEDGGIVLNEVNTMPGFTSYSRYPRMVAAAGITLPALIDS
                     LITLALKR"
     gene            2045..2653
                     /locus_tag="IWM23_003151"
     CDS             2045..2653
                     /locus_tag="IWM23_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-B"
                     /protein_id="HAP9885689.1"
                     /translation="MENGFLFLDEMLHGVRWDAKYATWDNFTGKPVDGYEVNRIIGTK
                     AVAFALREAQIHAAALGYGLLLWDGYRPRTAVDCFLRWAAQPEDNLTKEKFYPNIERA
                     ELITKGYVASQSSHSRGSAIDLTLYHLDTGELVSMGSNFDFMDERSHHTAKGIGDAEA
                     QNRRCLRKIMESSGFQSYRFEWWHYKLIDEPYPDTYFNFAVS"
BASE COUNT          764 a          573 c          777 g          686 t
ORIGIN      
        1 gaataaaaac tattagacaa atgatatttt acgaggagga gaaccaatga gaaaaagtgt
       61 gggcattact gtttttggat gcgaacagga tgaggcaaat gctttccgcg ctttatcgcc
      121 ggattttcat attatcccta cgctgattag cgatgcgata tcggcagaca acgcaaaatt
      181 ggccgctggc aatcaatgcg ttagcgtagg ccataagtcc gaggtttccg aggcgacaat
      241 tcttgcgctg agaaaggtcg gggtaaaata catttctacc cgcagcatcg gctgcgatca
      301 cattgatacg actgccgccg agagaatggg aatctcggtt ggcacggttg cgtattcgcc
      361 ggacagcgtt gcggattatg ctttgatgct gatgctgatg gccatacggg gtgcaaaacc
      421 caccatgcac gccgtggcgc aacaagattt cagattggat cgtatccggg ggaaagaact
      481 gggggatatg actgtgggag ttattggaac cggccatatc gggcaagcgg tcgtcaaaag
      541 gctgcgggga tttggatgcc atgtgctggc ctatgataac agccgaaaaa tggatgcaga
      601 ttatgtccag cttgatgagc ttctaaaaaa cagcgatatt gttacgctcc atgtgccgct
      661 ttgtgcggat acccgccatc tgatcggtca gaagcaaatt ggagagatga agcaaggcgc
      721 atttttaatc aacaccgcgc gcggagcgct tgtcgatacc ggggcgctgg tggaggcgtt
      781 ggaaagcgga aagctgggtg gtgcggcact ggatgtgttg gagggcgagg atcagtttgt
      841 ttataccgat tgctcgcaga aagtgcttga ccatcccttt ttgtcgcagc ttttaaggat
      901 gccgaatgtg atcatcacac cccatacggc gtactacact gagcgtgtgc tgcaggatac
      961 tacagaaaaa acaatcagga attgtctcaa ttttgaaagg agtttacagc atgaatagaa
     1021 taaaagtcgc aatcatcttc ggcggttgct cggaggaaca tgatgtgtcg gtaaaatccg
     1081 caatagaaat tgctgcgaac attgatacgg aaaaattcga tccgcactac atcggaatta
     1141 caaaaaacgg tgtatggaag ctatgcaaga agccatgtac ggaatgggaa gccgacagtc
     1201 tccccgccat actctccccg gataggaaaa cgcatgggct gcttgtcatg aaagaaagcg
     1261 aatacgaaac acggcgtatt gatgtggctt tcccggtttt gcatggcaaa tgcggggagg
     1321 atggtgcgat acaggggctg tttgtattgt ctggtatccc ctatgtgggc tgtgatattc
     1381 aaagctccgc agcttgcatg gacaaatcac tggcctacat tcttacaaaa aatgcgggca
     1441 tcgccgttcc cgaatttcaa attattgata aaggtgacaa gccggaggcg ggtgcgctta
     1501 cctaccctgt ctttgtgaag ccggcacggt caggttcgtc ctttggcgta accaaagtaa
     1561 acggtacgga agaacttaac gctgcgatag aagcggcagg acaatatgat ggaaaaatct
     1621 taattgagca agcgatttcg ggctgtgagg tcgggtgtgc ggtcatgggg aacgaggatg
     1681 atttgattgt cggcgaagtg gatcaaatcc ggctgagcca cggtatcttc cgcatccatc
     1741 aggaaaacga gccggaaaaa ggctcagaaa atgcgatgat tacagttcac gcagacattc
     1801 cggtcgagga acgaaatcgg gtgcaggaaa cggcaaagaa agtatatcgg gtgcttggat
     1861 gcagagggct tgcccgtgtt gatctttttt tgcaggagga tggcggcatc gttctaaatg
     1921 aggtcaatac catgcccggt tttacatcgt acagccgcta cccacgtatg gtggccgccg
     1981 caggaatcac gcttcctgca ctgattgaca gcctgattac attggcgtta aagaggtgat
     2041 aagtatggaa aatggttttt tgtttttaga tgaaatgttg catggcgtcc gttgggatgc
     2101 caagtacgcc acatgggata acttcacagg aaaaccggtg gatgggtatg aggtgaatcg
     2161 catcatcggc acaaaggccg tggcgtttgc tctgcgcgag gcacaaatcc atgcggctgc
     2221 gcttggctat ggcttgcttt tatgggatgg atatcggcca agaactgcgg tggactgctt
     2281 cctgcgttgg gcagcgcaac cggaggacaa tctcacaaaa gaaaaatttt accccaatat
     2341 agagcgagcc gagttgatta caaagggtta tgtggcctca caatccagcc atagccgtgg
     2401 aagcgcaatt gatcttacgc tctaccacct ggatacaggg gaacttgttt caatgggaag
     2461 taacttcgat tttatggacg aacggtcgca tcatacagca aaagggatag gggatgcaga
     2521 ggcacaaaat cgaagatgct tgcgtaaaat catggaaagc agcggatttc agtcttatcg
     2581 ctttgaatgg tggcactata agttgattga tgagccatac cccgatacct attttaattt
     2641 tgccgtttca taatgaaagt attggttttg tatcagattt tggatttgat aaaatctatg
     2701 ctggtcatgg acaatctttt actggaaagt ggttaaagca tcttttgtaa tacattgcaa
     2761 attatagtat tcaatctgcc cagcggcaga aaagaaaaaa
//
LOCUS       DACDXE010000265         2440 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.169, whole genome shotgun sequence.
ACCESSION   DACDXE010000265 DACDXE010000000
VERSION     DACDXE010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.169"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IWM23_003152"
     CDS             <1..75
                     /locus_tag="IWM23_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9885690.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="IWM23_003153"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="IWM23_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9885691.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            2129..2296
                     /locus_tag="IWM23_003154"
     CDS             2129..2296
                     /locus_tag="IWM23_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885692.1"
                     /translation="MCPVCGNKTRLKIREDTELKKFPLYCPKCRQENLIEIKQFKVTV
                     ITEPDAKTQSR"
     gene            complement(2356..>2440)
                     /locus_tag="IWM23_003155"
     CDS             complement(2356..>2440)
                     /locus_tag="IWM23_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9885693.1"
                     /translation="MDSFTDKELSLMESLASGINEARNIED"
BASE COUNT          838 a          370 c          488 g          744 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcgtttc aaaatgcagt tatggagggg atacgctatg gctgtgaaca aggattgtat
     1561 ggttggaatg tgacggactg taaaatctgt tttaagtatg gcttatacta tagccctgtt
     1621 agtaccccag cagattttcg gatgcttgct cctattgtat tggaacaagt cttaaaaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat
     1741 ctttcacgag catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg
     1801 aaaaataatg aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt
     1861 agtgatttaa ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggtac
     1921 catgttacta ccggtgaacc tgtttgccag ccccgtcgtc caaatagtcg gatagataaa
     1981 gtacgatata tgttcaataa aataacttag tgtattttat gttgttatat aaatatggtt
     2041 tcttgttaaa taagatgaaa tattttttaa taaagatttg aattaaagtg taaaggagga
     2101 gatagttatt ataaactaca agtggatatt gtgtcctgta tgtggaaata aaacacgatt
     2161 aaagataagg gaagatactg aattaaaaaa attccccctc tattgtccga aatgcagaca
     2221 agaaaattta attgaaataa agcagttcaa agtaactgtg attacagagc cagacgcaaa
     2281 gacgcagagc cgataaaatg agattaatac aatctcattt tatcggctct ttccgttatg
     2341 tatggattct tttaattagt cttcgatgtt tcttgcttcg ttgataccgc tggctaaaga
     2401 ttccattaag gatagttctt tgtctgtaaa gctatccatg
//
LOCUS       DACDXE010000266         2438 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.170, whole genome shotgun sequence.
ACCESSION   DACDXE010000266 DACDXE010000000
VERSION     DACDXE010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.170"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..75
                     /locus_tag="IWM23_003156"
     CDS             <1..75
                     /locus_tag="IWM23_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAP9885694.1"
                     /translation="PMVMHKNPSDKSIYHWDFYALLGF"
     gene            91..2010
                     /gene="tet(M)"
                     /locus_tag="IWM23_003157"
     CDS             91..2010
                     /gene="tet(M)"
                     /locus_tag="IWM23_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAP9885695.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIV
                     NTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRL
                     NSRIDKVRYMFNKIT"
     gene            2108..2296
                     /locus_tag="IWM23_003158"
     CDS             2108..2296
                     /locus_tag="IWM23_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAP9885696.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(2354..>2438)
                     /locus_tag="IWM23_003159"
     CDS             complement(2354..>2438)
                     /locus_tag="IWM23_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAP9885697.1"
                     /translation="MDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          839 a          372 c          473 g          754 t
ORIGIN      
        1 cctatggtta tgcataaaaa tcccagtgat aaaagtattt atcactggga tttttatgcc
       61 cttttgggtt tttgaatgga ggaaaatcac atgaaaatta ttaatattgg agttttagct
      121 catgttgatg caggaaaaac taccttaaca gaaagcttat tatataacag tggagcgatt
      181 acagaattag gaagcgtgga caaaggtaca acgaggacgg ataatacgct tttagaacgt
      241 cagagaggaa ttacaattca gacaggaata acctcttttc agtgggaaaa tacgaaggtg
      301 aacatcatag acacgccagg acatatggat ttcttagcag aagtatatcg ttcattatca
      361 gttttagatg gggcaattct actgatttct gcaaaagatg gcgtacaagc acaaactcgt
      421 atattatttc atgcacttag gaaaatgggg attcccacaa tcttttttat caataagatt
      481 gaccaaaatg gaattgattt atcaacggtt tatcaggata ttaaagagaa actttctgcc
      541 gaaattgtaa tcaaacagaa ggtagaactg tatcctaata tgtgtgtgac gaactttacc
      601 gaatctgaac aatgggatac ggtaatagag ggaaacgatg accttttaga gaaatatatg
      661 tccggtaaat cattagaagc attggaactc gaacaagagg aaagcataag atttcagaat
      721 tgttctctgt tccctcttta tcatggaagt gcaaaaagta atatagggat tgataacctt
      781 atagaagtta ttactaataa attttattca tcaacacatc gaggtccgtc tgaactttgc
      841 ggaaatgttt tcaaaattga atatacaaaa aaaagacaac gtcttgcata tatacgcctt
      901 tatagtggag tactacattt acgagattcg gttagagtat cagaaaaaga aaaaataaaa
      961 gttacagaaa tgtatacttc aataaatggt gaattatgta agattgatag agcttattct
     1021 ggagaaattg ttattttgca aaatgagttt ttgaagttaa atagtgttct tggagataca
     1081 aaactattgc cacagagaaa aaagattgaa aatccgcacc ctctactaca aacaactgtt
     1141 gaaccgagta aacctgaaca gagagaaatg ttgcttgatg cccttttgga aatctcagat
     1201 agtgatccgc ttctacgata ttacgtggat tctacgacac atgaaattat actttctttc
     1261 ttagggaaag tacaaatgga agtgattagt gcactgttgc aagaaaagta tcatgtggag
     1321 atagaactaa aagagcctac agtcatttat atggagagac cgttaaaaaa tgcagaatat
     1381 accattcaca tcgaagtgcc gccaaatcct ttctgggctt ccattggttt atctgtatca
     1441 ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg tttctcttgg atacttaaat
     1501 caatcatttc aaaatgcagt tatggaaggg atacgctatg gttgcgaaca aggattatat
     1561 ggttggaatg tgacggattg taaaatctgt tttaagtacg gtttatacta tagccctgtt
     1621 agtactccag cagattttcg gatgcttact cctattgtac tggagcaagc ctttagaaaa
     1681 gctggaacag aattgttaga gccatatctt agttttaaag tttatgcacc acaggaatat
     1741 ctttcacggg catataacga tgctcccaaa tattgtgcaa atatcgtaaa tactcaactg
     1801 aaaaataatg aggtcattat tattggagaa attcctgctc gatgtattca agattatcgc
     1861 aatgatttaa ctttttttac aaatgggctt agtgtttgtt tagcagagct aaaaggatat
     1921 caggttacca ctggcgaacc tgtttgccag acccgtcgtc taaatagtcg gatagataaa
     1981 gtaagatata tgttcaataa aataacttag tgcgttttat gttgttatat aaatatggtt
     2041 tcttattaaa taagatgaaa tattctttaa tatagatttg aattaaagtg gaaaggagga
     2101 gattgttatt ataaactaca agtggatatt gtgtcctatt tgtggaaata aaacaagact
     2161 acgaatacga gtggatacta tacttaaaaa tttcccttta tactgcccca aatgtaagaa
     2221 cgaaacttta attaatgttc aaaaaatgaa tataataaca atcaaagagc cagacgccaa
     2281 gacgcagagc cgataatttg agaaatgaaa ctctcatctt atcggctctt tttgtttatc
     2341 tgaattttac tgactagcct tcaatatttc ttgcttcatt gatacctttt gctagtgctt
     2401 ccattaagga tagttctttg tctgtaaagc tatccatg
//
LOCUS       DACDXE010000267         2153 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.177, whole genome shotgun sequence.
ACCESSION   DACDXE010000267 DACDXE010000000
VERSION     DACDXE010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.177"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            170..1648
                     /gene="msr"
                     /locus_tag="IWM23_003160"
     CDS             170..1648
                     /gene="msr"
                     /locus_tag="IWM23_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAP9885698.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            1695..2141
                     /locus_tag="IWM23_003161"
     CDS             1695..2141
                     /locus_tag="IWM23_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAP9885699.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
BASE COUNT          723 a          352 c          465 g          613 t
ORIGIN      
        1 ttcgaacgac agtagaatag gtgaaagcta tgacagattt ggtgatttag tcatagttat
       61 ttcgttcaga gtaggaaaag atgtgatgca gtttttttgc aaacagattt ttctgctctt
      121 ttttagtatc gataattgtt gttttacatg aaataggagt gtaaaacata tggaaaattt
      181 agcagtaaat ataacaaatc tacaagtcag ctttggcaac cagctagaac tatctattga
      241 ttctcttcgt gtctatcagc aagatcgtat agggatcata ggagaaaatg gtgtcggtaa
      301 atcgactttg ctcaaactaa tagccggtga actttttccc gatcatggga aaatccaaac
      361 agagatcacc ttcaactacc tgcctcaatt aacctatctc gctgaagcaa aggacctaaa
      421 tttggaatta gccagtcatt tccagttaag actggaagaa acttcagagc ggaaatggag
      481 cggaggggaa gaacgaaaga tcgagttgat acgtcttctt tcttcttatg aacaagggat
      541 gcttctagac gagccaacaa cccatctaga tagaaaaagt attgatcgac tgattgaaga
      601 gcttcgttat tattatggta cgctggtttt tgttagtcat gatcgctatt ttctagatga
      661 gctagcatcg aaaatctggg aagtaaaaga cggagaaatc cgagagtttt cggggaatta
      721 tagtgcctat ctcactcaaa aggaattgga gaaaaagact cagctacgag aagcagagtc
      781 gatcatgaaa gagaaaaaac gattggaaaa atcgatccaa gaaaagaaaa aacaagcgga
      841 aaagttggaa aaagtgtcca gtaaaaagaa aaagcaacaa atcagactgg atcggttgtc
      901 ttcctctaaa caaaaagaca gtgtacaaaa agccatccaa aagaatgcga aaacattaga
      961 gagaagactc caaaaaatag gagaaacaac caagccgcag cagatgaaac aaatccgttt
     1021 tccagtacca aaatctcttg aactccacag ccgctatccc atcatgggac aaaatgtcca
     1081 attggaaaga agtgggagaa cattactggt aaatggtgat tttcagtttt ctttaggtaa
     1141 aaaaatcgcg attgtcggtg aaaatggttc aggtaagaca accttattag aacatatccg
     1201 caaacaagga gaaggaatcc ttctctctcc gaaagtaagc tttcaagtat atcagcaaaa
     1261 gggttatcaa atgacatctg aagaatccat cattcgtttt gtcatgagac aaacagagtt
     1321 ttcagaatcg cttgtccgta gtttgctgaa tcacttaggg tttgctcagg aaactttgac
     1381 gaaaccgtta tgtacattaa gtgggggaga agcgacccgt ctgacgattg ctttgctttt
     1441 tactaagcca agtaatgtgt tgctgttaga tgaaccgact aattttattg atatggcaac
     1501 gatcgaagct ttagagaagc taatgcaaat atatccggga acgattttgt ttacttctca
     1561 tgattcttac tttgtcgagc gtacggctga tgaagtttat gaaataaaag ggcagaaaat
     1621 aaaaaaagta cttacgagaa atttttaaat gtgctacgat gattttcgtg acaaatttaa
     1681 aggagtggta cagattgcct acaaataaaa aagaaggaat aatttttaca acaatgatgt
     1741 gttttttgat ggtcttgggg atgagcgcgt ataatttatg gctgcatcaa gatcttcatt
     1801 tatcaagttt gttgattggt ctagtgcctg gattcgtggt ggctttcatt ttggatgtct
     1861 tcatcatcgg tgtgctggcg aaaaaaattg cctttaaact accagtaaat ccagaaagca
     1921 aggtccaaat gatcttaact atttcatgct tgatggtttt aggaatggtg acatgtatgt
     1981 ctttattcgg tgtgatcatg gaacaaggaa tacctgatca attattctct gcttatatga
     2041 atgcttggaa aatgaatgtg attgccgctt taccgttgca gttgatcatc gttggcccgc
     2101 tttcaagaag tgtgctaaaa gcaattcaaa gtaatgaatg atggaacact gtt
//
LOCUS       DACDXE010000268         1790 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.190, whole genome shotgun sequence.
ACCESSION   DACDXE010000268 DACDXE010000000
VERSION     DACDXE010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.190"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            121..927
                     /gene="vanY"
                     /locus_tag="IWM23_003162"
     CDS             121..927
                     /gene="vanY"
                     /locus_tag="IWM23_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-B/VanY-G family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HAP9885700.1"
                     /translation="MEKSNYHSNADHHKRHMKQSVEKRAFLCAFIISFTVCTLFLGRR
                     LASVLEATQIPPIPATHTGSSTDVVENLEENALATAKEQGDEQEWSLILVNRQNPIPA
                     QYDVELEQLSNGERIDIRISPYLQDLFDAARTDGVYPIVASGYRTTEKQQEIMDEKIA
                     EYKAKGYTSAQAKAEAETWVAVPGTSEHQLGLAVDINADGIHSTGNEVYRWLDENSYR
                     FGFIRRYPPDKTEITGVSNEPWHYRYVGIEAATEMYNQGVCLEEYLKPEK"
     gene            940..1776
                     /gene="vanW"
                     /locus_tag="IWM23_003163"
                     /pseudo
     CDS             940..1776
                     /gene="vanW"
                     /locus_tag="IWM23_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010815683.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance accessory protein
                     VanW-B"
BASE COUNT          556 a          373 c          443 g          418 t
ORIGIN      
        1 ttttttgctg ctacaggaaa actacagact ggctacaggg aaagtacaga tgcgcttgcc
       61 ataataacaa tcgtaccagc cacaaatcgt agttttattg caaaggaggc attcgatcaa
      121 atggaaaaaa gcaactatca ttccaatgcg gatcatcaca aacggcatat gaaacaatcc
      181 gtggaaaagc gggcttttct atgtgcgttc attatttcgt tcacagtctg cacgctgttt
      241 ttggggcgga gactggcttc cgtattggag gcaacacaga taccgcccat ccctgcaact
      301 catacaggca gcagcactga cgtagtggag aatttggagg aaaacgctct tgccaccgcc
      361 aaagaacagg gagatgaaca ggaatggagc ctgattttag tgaacaggca gaaccccatc
      421 cccgcacagt acgatgtgga gcttgagcaa ctatcaaatg gtgagcggat agatattcgg
      481 atttctccct atcttcaaga tttgtttgat gccgcaagaa ctgatggagt ttacccgatt
      541 gtcgcatccg gataccgaac aacagaaaaa cagcaagaaa ttatggatga aaaaattgcc
      601 gaatataagg cgaaaggcta cacctctgca caggctaaag cggaagcaga aacttgggtg
      661 gccgtgccgg gaacgagcga gcatcagctt ggtcttgctg tggatatcaa tgcggacgga
      721 attcattcaa caggcaacga ggtttataga tggctggatg aaaacagcta tcgttttggt
      781 tttattcgtc gctacccacc agacaagaca gagataaccg gtgtgagcaa cgagccgtgg
      841 cattaccgat atgttggcat cgaagctgcc acagagatgt acaaccaagg ggtttgcctt
      901 gaggaatatt taaaaccaga aaaatgagaa aaggatgtta tggacagaaa aagattgaca
      961 cagcgtttcc cgttcctgct tccaatgaga cgggcgcaga gaaaaatgtg cttttatgcg
     1021 ggaatgagat ttgacggctg tcgctatgcg cagacgatag gggaaaaatc gctttcccat
     1081 ttgctctttg aaacggattg cgcattatac aaccacaata ccggatttga catgatatac
     1141 caggaaaaca aggtgttcaa cttaaagctg gcagcaaaga ccttaaacgg cttattgata
     1201 agaccggggg aaaccttttc tttctggtgg ctggtacgcc atgcggacaa agataccccc
     1261 tataaagacg gccttacggt gaccaatggt aaactcacca ccatgtcggg cggcggtatg
     1321 tgccagatga gcaatttact attttggatg ttcctgcata cgccattgac aattatccag
     1381 cgcagaggtc acgaagtaaa ggagtttcca gagccaaaca gtgacgagat taaaggggtg
     1441 gatgcaacca tttcagaggg ctggattgat ttaaaagtgc gaaacgatac cgactgcacc
     1501 taccaaatat gagtgacctt agatgatgag aaaatcatcg gtcaggtgtc cgccgacaaa
     1561 gagccccaag cattatacaa aattacaaat ggcagtatcc agtatgtccg tgaaagtggc
     1621 gggatttatg aatatgccca ggttaaacgg atgcaagttg ccttaggtac cggggaaata
     1681 atagattgca agctgcttta tacaaacaaa tgcaaaatct gttatcccct cccggaaagt
     1741 gtggatattc agtctaatgg tgataagtca agatgaaatt taagattttc
//
LOCUS       DACDXE010000269         1781 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.191, whole genome shotgun sequence.
ACCESSION   DACDXE010000269 DACDXE010000000
VERSION     DACDXE010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.191"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            24..416
                     /locus_tag="IWM23_003164"
     CDS             24..416
                     /locus_tag="IWM23_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAP9885701.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWM23_003165"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IWM23_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAP9885702.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACDXE010000270         1504 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.200, whole genome shotgun sequence.
ACCESSION   DACDXE010000270 DACDXE010000000
VERSION     DACDXE010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.200"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            262..373
                     /locus_tag="IWM23_003166"
                     /pseudo
     CDS             262..373
                     /locus_tag="IWM23_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008787626.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="IWM23_003167"
     CDS             394..477
                     /locus_tag="IWM23_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HAP9885703.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM23_003168"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="IWM23_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004292767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HAP9885704.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DACDXE010000271         1382 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.205, whole genome shotgun sequence.
ACCESSION   DACDXE010000271 DACDXE010000000
VERSION     DACDXE010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.205"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            <1..1311
                     /locus_tag="IWM23_003169"
     CDS             <1..1311
                     /locus_tag="IWM23_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HAP9885705.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DACDXE010000272         1349 bp    DNA     linear   BCT 12-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium isolate E20904_2017
            SAMN12640147-rid9881573.guided.208, whole genome shotgun sequence.
ACCESSION   DACDXE010000272 DACDXE010000000
VERSION     DACDXE010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN12640147
            Sequence Read Archive: SRR10023401
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAY-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 20-MAY-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000741926.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000741926.1
            Genome Coverage       :: 46x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/21/2020 02:40:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,171
            CDSs (total)                      :: 3,109
            Genes (coding)                    :: 3,025
            CDSs (with protein)               :: 3,025
            Genes (RNA)                       :: 62
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 84
            CDSs (without protein)            :: 84
            Pseudo Genes (ambiguous residues) :: 0 of 84
            Pseudo Genes (frameshifted)       :: 31 of 84
            Pseudo Genes (incomplete)         :: 44 of 84
            Pseudo Genes (internal stop)      :: 18 of 84
            Pseudo Genes (multiple problems)  :: 8 of 84
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN12640147-rid9881573.guided.208"
                     /isolate="E20904_2017"
                     /isolation_source="environmental"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Australia"
                     /lat_lon="32.067224 S 115.830063 E"
                     /collection_date="2017"
                     /collected_by="Murdoch University"
     gene            46..297
                     /locus_tag="IWM23_003170"
                     /pseudo
     CDS             46..297
                     /locus_tag="IWM23_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="IWM23_003171"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="IWM23_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HAP9885706.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          424 a          234 c          322 g          369 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 actgtccata ctctgatgtt ttatatcttt tctaaaagtt cgctagatag gggtcccgag
     1321 cgcctacgag gaatttgtat cgataagaa
//
